title: A novel biologic hemostatic dressing (fibrin patch) reduces blood loss and resuscitation volume and improves survival in hypothermic, coagulopathic Swine with grade V liver injury.
abstract: OBJECTIVE
  This study evaluated the efficacy of a biologic hemostatic fibrin patch (FP) to control coagulopathic bleeding and prevent death in a porcine model of severe liver injury with hemodilution and hypothermia.
  
  
  METHODS
  Coagulopathy was produced in swine by exchanging 60% of the animals' circulating blood volume with Hextend and lowering the core temperature to 32.0 degrees C +/- 0.5 degrees C. A grade V liver injury was induced and allowed to bleed freely for 30 seconds (pretreatment blood loss). Animals were randomly divided into three treatment groups: hepatic packing (HP) using laparotomy sponges, FP application plus HP, or placebo patch (PP) application plus HP. Animals were resuscitated to 80% of the preinjury mean arterial pressure. Core temperature, mean arterial pressure, and survival were monitored for 1 hour postinjury. Packs were removed from the animals that survived to 1 hour and they were monitored for an additional hour.
  
  
  RESULTS
  Coagulopathy was confirmed by significant increases (p < 0.01) in prothrombin time, activated partial thromboplastin time, and activated clotting time in preinjury measurements as compared with baseline values. Pretreatment blood loss was not different among the groups. However, significant (p < 0.01) differences were observed in the posttreatment blood loss (772 mL +/- 340 mL, 4,977 mL +/- 440 mL, 4,173 mL +/- 608 mL), as well as the required fluid resuscitation volume (994 mL +/- 26 mL, 4,083 mL +/- 185 mL, 3,494 mL +/- 492 mL), between FP versus PP or HP groups, respectively. In addition, 89% of FP animals survived the 2-hour observation with an average survival time of 111 minutes +/- 9 minutes, which was significantly higher than the PP (0% survival, 39 minutes +/- 4 minutes) or HP (13% survival, 41 minutes +/- 12 minutes) groups.
  
  
  CONCLUSIONS
  FP with packing effectively controlled coagulopathic bleeding and prevented death in a model of grade V liver injury in which HP alone (standard of care) was ineffective.
authors.affiliation: US Army Institute of Surgical Research, San Antonio, Texas, USA. angel.delgado@amedd.army.mil
authors.forename: Angel V
authors.initials: AV
authors.lastname: Delgado
authors.forename: Bijan S
authors.initials: BS
authors.lastname: Kheirabadi
authors.forename: Todd M
authors.initials: TM
authors.lastname: Fruchterman
authors.forename: Michael
authors.initials: M
authors.lastname: Scherer
authors.forename: Douglas
authors.initials: D
authors.lastname: Cortez
authors.forename: Charles E
authors.initials: CE
authors.lastname: Wade
authors.forename: Michael A
authors.initials: MA
authors.lastname: Dubick
authors.forename: John B
authors.initials: JB
authors.lastname: Holcomb
mesh_terms: D000818:Animals; D001458:Bandages; D001778:Blood Coagulation Disorders / Q000150:complications; D001780:Blood Coagulation Tests; D004195:Disease Models, Animal; D005260:Female; D015718:Fibrin Tissue Adhesive; D005440:Fluid Therapy; D006470:Hemorrhage / Q000209:etiology / Q000628:therapy*; D006489:Hemostatic Techniques; D006490:Hemostatics; D007035:Hypothermia / Q000150:complications; D008099:Liver / Q000293:injuries*; D008297:Male; D012151:Resuscitation; D012771:Shock, Hemorrhagic; D034421:Sus scrofa
chemical_list: D015718:Fibrin Tissue Adhesive; D006490:Hemostatics