title: The Na, K-ATPase alpha3-isoform specifically localizes in the Schmidt-Lanterman incisures of human nerve.
abstract: BACKGROUND
  To our knowledge, there is little reference in literature with regards to alpha3-isoform of Na+,K+-ATPase in human peripheral nerves. The aim of this study was to determine the expression of the neuronal alpha3-isoform of Na+,K+-ATPase in human sural nerves from patients with a permanent medullary central nervous system injury.
  
  
  METHODS
  We studied the immunolocalization of alpha3-isoform of Na+,K+-ATPase using a polyclonal antibody against the amino sequence near the phosphorylation site of the alpha3-isoforms of Na+,K+-ATPase using immunohistochemistry and confocal laser scanning microscopy. An antibody specific for alpha2-isoform of Na+,K+-ATPase was used to label the Schwann cells.
  
  
  RESULTS
  Morphometric analysis of longitudinal section of human sural nerves showed that the alpha3-isoform of Na+,K+-ATPase was distributed along the length of axolemma. The myelin sheath of the Schwann cells showed clearly a distribution of alpha3- but not alpha2-isoforms of Na+,K+-ATPase at the level of Schmidt-Lanterman incisures.
  
  
  CONCLUSIONS
  The human sural nerve shows a specific localization of the Na+,K+-ATPase alpha3-isoform in the Schmidt-Lanterman incisures of Schwann cells in addition to its localization in axonal membranes.
authors.affiliation: Inserm U927, Faculté de Médecine, Université de Poitiers France.
authors.forename: P
authors.initials: P
authors.lastname: Rigoard
authors.forename: F
authors.initials: F
authors.lastname: Tartarin
authors.forename: K
authors.initials: K
authors.lastname: Buffenoir
authors.forename: M
authors.initials: M
authors.lastname: Chaillou
authors.forename: M
authors.initials: M
authors.lastname: Fares
authors.forename: S
authors.initials: S
authors.lastname: D'Houtaud
authors.forename: M
authors.initials: M
authors.lastname: Wager
authors.forename: J P
authors.initials: JP
authors.lastname: Giot
authors.forename: N
authors.initials: N
authors.lastname: Quellard
authors.forename: B
authors.initials: B
authors.lastname: Fernandez
authors.forename: F
authors.initials: F
authors.lastname: Lapierre
authors.forename: J M
authors.initials: JM
authors.lastname: Maixent
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009186:Myelin Sheath / Q000201:enzymology; D011487:Protein Conformation; D012583:Schwann Cells / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*; D013497:Sural Nerve / Q000201:enzymology*
chemical_list: C488756:ATP1A3 protein, human; D000254:Sodium-Potassium-Exchanging ATPase