Sigma factor SigC is required for heat acclimation of the cyanobacterium Synechocystis sp. strain PCC 6803.
The role of the primary-like sigma factor SigC was studied in Synechocystis. Under high temperature stress (48 degrees C) the DeltasigC inactivation strain showed a lower survival rate than the control strain. The DeltasigC strain grew poorly at 43 degrees C in liquid cultures under normal air. However, change to 3% CO(2) enhanced growth of DeltasigC at 43 degrees C. Differences in expression of many genes related to the carbon concentrating mechanisms between the control and the DeltasigC strain were recorded with a genome-wide DNA microarray. We suggest that low solubility of CO2 at high temperature is one of the factors contributing to the poor thermotolerance of the DeltasigC strain.
Tuominen
Ilona
I
Plant Physiology and Molecular Biology, Department of Biology, University of Turku, Turku, Finland.
Pollari
Maija
M
Aguirre von Wobeser
Eneas
E
Tyystjärvi
Esa
E
Ibelings
Bas W
BW
Matthijs
Hans C P
HC
Tyystjärvi
Taina
T
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D006358:Hot Temperature; D020411:Oligonucleotide Array Sequence Analysis; D012808:Sigma Factor / Q000502:physiology*; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D001426:Bacterial Proteins; D017931:DNA Primers; D012808:Sigma Factor; C111259:sigE protein, Bacteria