title: Comparative analysis of whole genome structure of Streptococcus suis using whole genome PCR scanning.
abstract: An outbreak associated with Streptococcus suis infection in humans emerged in Sichuan province, China in 2005. The outbreak is atypical for the apparent large number of human cases, high fatality rate and geographical spread. To determine whether the bacterium has changed, we compared both human and animal isolates from the Sichuan outbreak with those collected previously within China and in other countries using whole genome PCR scanning (WGPScaning) comparative sequencing of several known virulence factor genes and multilocus sequence typing (MLST) analysis. WGPScanning analysis showed that all primer pairs yielded PCR products of the expected sizes in all four strains tested. The nucleotide sequences of all the detected virulence factor genes are identical in the four strains and MLST results showed that the four isolates studied and reference strain all belonged to the ST1 complex. No new genetic changes were found in the genome structure of the isolates from this Sichuan outbreak.
authors.affiliation: State Key Lab for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100176, China.
authors.forename: ZhaoHui
authors.initials: Z
authors.lastname: Xiong
authors.forename: CanDong
authors.initials: C
authors.lastname: Wei
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: JunPing
authors.initials: J
authors.lastname: Peng
authors.forename: XingYe
authors.initials: X
authors.lastname: Xu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D016680:Genome, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017011:Streptococcus suis / Q000235:genetics* / Q000472:pathogenicity