title: Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.
abstract: We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.
authors.affiliation: Department of Genetics and Case Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunguang
authors.initials: C
authors.lastname: Guo
authors.forename: Yueting
authors.initials: Y
authors.lastname: Chen
authors.forename: Hennady P
authors.initials: HP
authors.lastname: Shulha
authors.forename: Michael P
authors.initials: MP
authors.lastname: Schnetz
authors.forename: Thomas
authors.initials: T
authors.lastname: LaFramboise
authors.forename: Cynthia F
authors.initials: CF
authors.lastname: Bartels
authors.forename: Sanford
authors.initials: S
authors.lastname: Markowitz
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Weng
authors.forename: Peter C
authors.initials: PC
authors.lastname: Scacheri
authors.forename: Zhenghe
authors.initials: Z
authors.lastname: Wang
mesh_terms: D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D000939:Epitopes; D011506:Proteins