title: High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.
abstract: A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.
authors.affiliation: Department of Surgery, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Polyak
authors.forename: Ilia
authors.initials: I
authors.lastname: Fishbein
authors.forename: Michael
authors.initials: M
authors.lastname: Chorny
authors.forename: Ivan
authors.initials: I
authors.lastname: Alferiev
authors.forename: Darryl
authors.initials: D
authors.lastname: Williams
authors.forename: Ben
authors.initials: B
authors.lastname: Yellen
authors.forename: Gary
authors.initials: G
authors.lastname: Friedman
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Levy
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000473:pathology; D001672:Biocompatible Materials / Q000737:chemistry; D002339:Carotid Arteries / Q000473:pathology; D002417:Cattle; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism*; D015316:Genetic Therapy / Q000379:methods; D008280:Magnetics; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D036103:Nanotechnology / Q000379:methods; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013232:Steel / Q000737:chemistry*; D015607:Stents
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D013232:Steel