title: Factor V Leiden mutation and type 1 diabetes mellitus.
abstract: Diabetes mellitus is considered to cause a tendency for arterial thrombosis. Recent studies addressed the association between venous and arterial disease. Resistance to activated protein C is one of the most common causes of venous thrombosis and linked to a single point mutation in the factor V gene, designated as factor V Leiden mutation. There is little information regarding the status of factor V Leiden mutation in type 1 diabetes. The aim of this study is to evaluate association among activated protein C sensitivity ratio, factor V Leiden mutation, and type 1 diabetes taking into account metabolic control, lipids and diabetic complications. The study population consisted of 47 healthy subjects (27.9 +/- 1.2 years) and 48 type 1 diabetic patients (27.9 +/- 1.1 years). Activated protein C sensitivity ratio was measured by activated partial thromboplastin time based assay. The presence of factor V Leiden mutation was determined by amplifying the fragments encompassing gene mutation by PCR. Mean normalized activated protein C sensitivity ratio values and prevalence of heterozygous factor V Leiden mutation were not significantly different between groups (1.08 +/- 0.03 and 6.3% in healthy subjects; 1.01 +/- 0.03 and 6.4% in type 1 diabetic patients, respectively). The activated protein C sensitivity ratio and factor V Leiden mutation were not found to be linked with metabolic control parameters, lipids and diabetic complications in type 1 diabetic patients. There was no association among factor V Leiden mutation, activated protein C sensitivity ratio and type 1 diabetes, metabolic control parameters as well as complications of diabetes. Although the propensity to thrombosis is increased in individuals with type 1 diabetes, activated protein C sensitivity ratio and factor V Leiden mutation do not appear to be significant determinants.
authors.affiliation: Department of Endocrinology and Metabolism, Baskent University Medical School, Ankara, Turkey. aslinar@hotmail.com
authors.forename: Asli Nar
authors.initials: AN
authors.lastname: Demirer
authors.forename: Mehmet
authors.initials: M
authors.lastname: Alikasifoglu
authors.forename: Ergul
authors.initials: E
authors.lastname: Tuncbilek
authors.forename: Sema
authors.initials: S
authors.lastname: Karakus
authors.forename: Tomris
authors.initials: T
authors.lastname: Erbas
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D015331:Cohort Studies; D048909:Diabetes Complications / Q000235:genetics*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics*; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male
chemical_list: C095381:factor V Leiden; D005165:Factor V