title: Cutaneous leishmaniasis in Nepal: Leishmania major as a cause.
abstract: Nepal is endemic for visceral leishmaniasis; however, recently a solitary report of cutaneous leishmaniasis (CL) was recorded. The present report describes a CL case in a Nepalese woman, initially referred as a case of basal cell carcinoma. Histopathological examination, culture and PCR analysis of lesions revealed it to be a case of CL. Molecular methods, including kDNA PCR, ITS-1 PCR-RFLP and sequence analysis, revealed the species as Leishmania major. The patient was cured with sodium stibogluconate. This report suggests that the extent to which L. major causes CL in the Indian subcontinent needs to be monitored.
authors.affiliation: Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi 110029, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Nasim Akhtar
authors.initials: NA
authors.lastname: Ansari
authors.forename: Singh
authors.initials: S
authors.lastname: Avninder
authors.forename: V
authors.initials: V
authors.lastname: Ramesh
authors.forename: Poonam
authors.initials: P
authors.lastname: Salotra
mesh_terms: D000818:Animals; D001706:Biopsy; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D018320:Leishmania major / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000469:parasitology* / Q000628:therapy; D008875:Middle Aged; D009390:Nepal / Q000453:epidemiology; D016133:Polymerase Chain Reaction