[Contamination of IgG preparation for intravenous use with IgA. II. Removal of IgA1 from IgG preparation using affinity chomatography on lectin jacalin columns].
Administration of preparations of IgG for intravenous use (IVIG) in individuals with high anti-IgA antibody levels in circulation is frequently accompained with anafilactoid reactions. Thus, there is a requirement for preparations of IVIG with very low levels of IgA. Reduction of IgA concentration in IgG preparations is generally difficult to achieve, since physical and chemical characteristics, on which proceses for Ig purification are based on, are similar for these two Igs. In this work the ability of lectin jacalin to selectively bind IgA1 subclass was exploited for removal of IgA1 from IVIG currently under development in Blood Transfusion Institute of Republic Serbia, Belgrade. Concentrations of Igs in this preparation of IVIG were measured by ELISA tests developed for these purposes. The results have shown 280 times decrease of IgA1 content without changes of IgG concentration. Therefore, affinity chomatography on jacalin column is a suitable method for removal of IgA1 subclass from IVIG. Because of a favourable pH and ionic strength conditions used in process, this procedure has no effect on native state of IgG.
Dovezenski
N
N
Velicković
Z
Z
Tosić
Lj
Lj
Nikolić
G
G
Popović
O
O
Romić
M
M
Djokić
M
M
Mijusković
Z
Z
D002846:Chromatography, Affinity; D004340:Drug Contamination; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D016756:Immunoglobulins, Intravenous / Q000737:chemistry*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous