A simple, sensitive and widely applicable ELISA for S100B: Methodological features of the measurement of this glial protein.
S100B expression, particularly extracellular S100B, is used as a parameter of glial activation and/or death in several situations of brain injury. Several immunoassays for S100B measurement are available, which differ with regard to specificity, sensitivity, sample application, and, of course, economic costs. We standardized two protocols for S100B measurement (range between 1.9pg and 10ng/mL) in human and rat samples from brain and adipose tissues, blood serum, cerebrospinal fluid, urine and cell culture. Abundance and secretion of this protein in adipose tissue reinforces the caution about its origin in blood serum. Interestingly, S100B recognition was affected by the redox status of the protein. This aspect should be considered in S100B measurement, assuming that oxidized and reduced forms possibly coexist in vivo and the equilibrium can be modified by oxidative stress of physiological or pathological conditions or even by obtaining sample conditions.
Leite
Marina Concli
MC
Programa de Pós-Graduação em Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Galland
Fabiana
F
Brolese
Giovana
G
Guerra
Maria Cristina
MC
Bortolotto
Josiane Woutheres
JW
Freitas
Rodrigo
R
Almeida
Lucia Maria Vieira de
LM
Gottfried
Carmem
C
Gonçalves
Carlos-Alberto
CA
D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009414:Nerve Growth Factors / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D009448:Neurochemistry / Q000379:methods*; D009457:Neuroglia / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid
D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C579160:S100B protein, human; C579158:S100b protein, rat