title: Adhesion to chondroitinase ABC treated dentin.
abstract: Dentin bonding relies on complete resin impregnation throughout the demineralised hydrophilic collagen mesh. Chondroitin sulphate-glycosaminoglycans are claimed to regulate the three-dimensional arrangement of the dentin organic matrix and its hydrophilicity. The aim of this study was to investigate bond strength of two etch-and-rinse adhesives to chondroitinase ABC treated dentin. Human extracted molars were treated with chondroitinase ABC and a double labeling immunohistochemical technique was applied to reveal type I collagen and chondroitin 4/6 sulphate distribution under field emission in-lens scanning electron microscope. The immunohistochemical technique confirmed the effective removal of chondroitin 4/6 sulphate after the enzymatic treatment. Dentin surfaces exposed to chondroitinase ABC and untreated specimens prepared on untreated acid-etched dentin were bonded with Adper Scotchbond Multi-Purpose or Prime and Bond NT. Bonded specimens were submitted to microtensile testing and nanoleakage interfacial analysis under transmission electron microscope. Increased mean values of microtensile bond strength and reduced nanoleakage expression were found for both adhesives after chondroitinase ABC treatment of the dentin surface. Adper Scotchbond Multi-Purpose increased its bond strength about 28%, while bonding made with Prime and Bond NT almost doubled (92% increase) compared to untreated specimens. This study supports the hypothesis that adhesion can be enhanced by removal of chondroitin 4/6 sulphate and dermatan sulphate, probably due to a reduced amount of water content and enlarged interfibrillar spaces. Further studies should validate this hypothesis investigating the stability of chondroitin 4/6 and dermatan sulphate-depleted dentin bonded interface over time.
authors.affiliation: Department of SAU and FAL, University of Bologna, Bologna, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Mazzoni
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ruggeri
authors.forename: Francesca
authors.initials: F
authors.lastname: Vita
authors.forename: Mirella
authors.initials: M
authors.lastname: Falconi
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lenarda
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D019765:Chondroitin ABC Lyase / Q000737:chemistry*; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D003871:Dermatan Sulfate / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission / Q000379:methods; D011509:Proteoglycans / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D013314:Stress, Mechanical; D013718:Tensile Strength; D014867:Water / Q000737:chemistry
chemical_list: D017220:Dentin-Bonding Agents; D011509:Proteoglycans; D019279:Resin Cements; D014867:Water; D003871:Dermatan Sulfate; C041330:Scotchbond; D019765:Chondroitin ABC Lyase