title: Nitric oxide spin-trapping and NADPH-diaphorase activity in mature rat brain after injury.
abstract: Traumatic brain injury (TBI) induces inflammatory reactions, and one of the essential mediators of this reaction is nitric oxide (NO). The action of this compound is still under study because no clear consensus has been reached about its exact action in the central nervous system. Further, it is unknown if, in the damaged brain, its neuroprotective activity outweighs its putative neurodegenerative properties. Using ferrous-diethyldithiocarbamate chelate, a lipophilic spin trap for NO detection by electron paramagnetic resonance (EPR) spectroscopy, we followed NO production in injured brain of mature Wistar rats. To relate changes in the amount of NO in the lesioned brain to the activity of NO synthase (NOS), this study also used NADPH-diaphorase staining. Our data show a rapid drop of NO concentration in the damaged brain below control values. This phenomenon persisted over several hours postinjury and varied with brain region. This decrease in NO concentration was accompanied by a simultaneous increase in the number of NADPH-diaphorase-positive cells, perhaps indicative of increased NOS activity. It is therefore assumed that, in the lesioned brain, a very rapid removal of NO occurs via its transformation to other reactive species such as peroxynitrite, which further adversely influence the damaged tissue.
authors.affiliation: Department of Neuroanatomy, Institute of Zoology, Jagiellonian University, Krakow, Poland. ziajam@poczta.fm
authors.forename: Marek
authors.initials: M
authors.lastname: Ziaja
authors.forename: Janusz
authors.initials: J
authors.lastname: Pyka
authors.forename: Anna
authors.initials: A
authors.lastname: Machowska
authors.forename: Anna
authors.initials: A
authors.lastname: Maslanka
authors.forename: Przemyslaw M
authors.initials: PM
authors.lastname: Plonka
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D019097:Spin Trapping
chemical_list: D005609:Free Radicals; D009569:Nitric Oxide; D001120:Arginine; D009252:NADPH Dehydrogenase