Structural studies on exopolysaccharides produced by three different propionibacteria strains.
The exopolysaccharides produced by three propionibacteria strains, Propionibacterium freudenreichii 109, Propionibacterium freudenreichii 111, and Propionibacterium thoenii 126, grown on whey-based media, were found to be charged heteropolymers, composed of D-glucose, D-mannose, and D-glucuronic acid in molar ratios of 2:2:1. By means of methylation analysis, mass spectrometry, partial acid hydrolysis, and 1D/2D NMR (1H and 13C) studies, it was determined that all three exopolysaccharides contain the same branched, pentasaccharide repeating unit: [Formula: see text].
Dobruchowska
Justyna M
JM
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Gerwig
Gerrit J
GJ
Babuchowski
Andrzej
A
Kamerling
Johannis P
JP
D000143:Acids / Q000737:chemistry; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D011424:Propionibacterium / Q000737:chemistry* / Q000378:metabolism
D000143:Acids; D011135:Polysaccharides, Bacterial