Deep reflection-mode photoacoustic imaging of biological tissue.
A reflection-mode photoacoustic (PA) imaging system was designed and built to image deep structures in biological tissues. We chose near-infrared laser pulses of 804-nm wavelength for PA excitation to achieve deep penetration. To minimize unwanted surface signals, we adopted dark-field ring-shaped illumination. This imaging system employing a 5-MHz spherically focused ultrasonic transducer provides penetration up to 38 mm in chicken breast tissue. At the 19-mm depth, the axial resolution is 144 microm and the transverse resolution is 560 microm. Internal organs of small animals were imaged clearly.
Song
Kwang Hyun
KH
Washington University in St. Louis, Department of Biomedical Engineering, Optical Imaging Laboratory, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130-4899, USA.
Wang
Lihong V
LV
D000162:Acoustics / Q000295:instrumentation; D000818:Animals; D002645:Chickens; D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007834:Lasers; D055095:Optics and Photonics / Q000295:instrumentation; D009928:Organ Specificity; D051381:Rats