title: Identification and analysis of a glutaryl-CoA dehydrogenase-encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB.
abstract: In this work, the gcdH gene from the denitrifying beta-proteobacterium Azoarcus sp. CIB was shown to encode a glutaryl-CoA dehydrogenase, which is essential for the anaerobic catabolism of many aromatic compounds and some alicyclic and dicarboxylic acids. The primary structure of the GcdH protein is highly conserved in many organisms. The divergently transcribed gcdR gene, encoding a LysR-type transcriptional regulator, accounts for the glutaconate/glutarate-specific activation of the Pg promoter driving expression of gcdH. The Azoarcus sp. CIBdgcdH mutant strain harbouring a disrupted gcdH gene was used as host to identify heterologous gcdH genes, such as that from Pseudomonas putida KT2440. Moreover, the expression of gcdH from P. putida can be efficiently controlled by the GcdR activator in Azoarcus sp. CIB, demonstrating the existence of cross-talk between GcdR regulators and gcdH promoters from members of different phylogenetic subgroups of proteobacteria.
authors.affiliation: Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas-CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Blas
authors.initials: B
authors.lastname: Blázquez
authors.forename: Manuel
authors.initials: M
authors.lastname: Carmona
authors.forename: José Luis
authors.initials: JL
authors.lastname: García
authors.forename: Eduardo
authors.initials: E
authors.lastname: Díaz
mesh_terms: D000693:Anaerobiosis; D020627:Azoarcus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D050770:Glutaryl-CoA Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D006841:Hydrocarbons, Aromatic; D050770:Glutaryl-CoA Dehydrogenase