Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes.
Delta-9-tetrahydrocannabinol (Delta(9)-THC), the main psychoactive component of marijuana, is known to dysregulate various immune responses. Cannabinoid (CB)-1 and -2 receptors are expressed mainly on cells of the central nervous system (CNS) and the immune system. The CNS is the primary target of cannabinoids and astrocytes are known to play a role in various immune responses. Thus we undertook this investigation to determine the global molecular effects of cannabinoids on normal human astrocytes (NHA) using genomic and proteomic analyses. NHA were treated with Delta(9)-THC and assayed using gene microarrays and two-dimensional (2D) difference gel electrophoresis (DIGE) coupled with mass spectrometry (MS) to elucidate their genomic and proteomic profiles respectively. Our results show that the expression of more than 20 translated protein gene products from NHA was differentially dysregulated by treatment with Delta(9)-THC compared to untreated, control NHA.
Bindukumar
B
B
Department of Medicine, Division of Allergy, Immunology, and Rheumatology, Buffalo General Hospital, University at Buffalo, State University of NY, Kaleida Health, 100 High Street, Buffalo, NY 14203, USA.
Mahajan
S D
SD
Reynolds
J L
JL
Hu
Z
Z
Sykes
D E
DE
Aalinkeel
R
R
Schwartz
S A
SA
D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000187:drug effects / Q000378:metabolism; D052276:Creatine Kinase, BB Form / Q000187:drug effects / Q000378:metabolism; D013759:Dronabinol / Q000494:pharmacology*; D005634:Fructose-Bisphosphate Aldolase / Q000187:drug effects / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D023281:Genomics; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009419:Nerve Tissue Proteins / Q000145:classification / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010751:Phosphopyruvate Hydratase / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics; D011619:Psychotropic Drugs / Q000494:pharmacology*
D018834:Chaperonin 60; D009419:Nerve Tissue Proteins; D011619:Psychotropic Drugs; D013759:Dronabinol; D005979:Glutathione Peroxidase; D052276:Creatine Kinase, BB Form; D005634:Fructose-Bisphosphate Aldolase; D010751:Phosphopyruvate Hydratase