title: [Chiral separation of tenatoprazole enantiomers using high performance liquid chromatography on vacomycin-bonded chiral stationary phase].
abstract: Vacomycin-bonded chiral stationary phase was used for the direct chiral separation of tenatoprazole enantiomers using reversed-phase high performance liquid chromatography (HPLC). The influences of the kinds and concentration of buffer and organic modifier, the pH value of buffer, column length and column temperature on the separation were examined. The chiral HPLC method for the separation of tenatoprazole enantiomers on a Chirobiotic V column (150 mm x 4.6 mm, 5 microm) was established with simplicity and good reproducibility using 0.02 mol/L ammonium acetate buffer (pH 6.0)-tetrahydrofuran (93:7, v/v) as the mobile phase at a flow rate of 0.5 mL/min and 20 degrees C. Under the above conditions, the enantiomers were separated on baseline with the resolution of 1.68. The relative standard deviations (RSDs) for the retention times of tenatoprazole enantiomers were 0.48% and 0.49% (n = 6). The RSDs for the peak areas of tenatoprazole enantiomers were 0.45% and 0.55% (n = 6).
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Guan
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yujin
authors.initials: Y
authors.lastname: Bi
authors.forename: Shuang
authors.initials: S
authors.lastname: Shi
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry* / Q000302:isolation & purification*; D015394:Molecular Structure; D009853:Omeprazole / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D013237:Stereoisomerism
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D007093:Imidazoles; D009853:Omeprazole; C119998:Tenatoprazole