title: Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies.
abstract: A water-soluble glycopeptide (GLPCW-II) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE-Sepharose Fast-Flow and Sephacryl S-300 High Resolution Chromatography. The glycopeptide had a molecular weight of 1.2x10(4)Da (determined by HPLC), and consisted of approximately 90% carbohydrate and approximately 8% protein as determined using the phenol-sulfuric acid method and the BCA protein assay reagent kit, respectively. The polysaccharide moiety was composed mainly of D-Glc, L-Fuc, and D-Gal in the ratio of 1.00:1.09:4.09. To facilitate structure-activity studies, the structure of the GLPCW-II polysaccharide moiety was elucidated using 1H and 13C NMR spectroscopy including COSY, TOCSY, HMBC, HSQC, and ROESY, combined with GC-MS of methylated derivatives, and shown to consist of repeating units with the following structure: [Formula: see text].
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
authors.forename: LiBin
authors.initials: L
authors.lastname: Ye
authors.forename: JingSong
authors.initials: J
authors.lastname: Zhang
authors.forename: XiJun
authors.initials: X
authors.lastname: Ye
authors.forename: QingJiu
authors.initials: Q
authors.lastname: Tang
authors.forename: YanFang
authors.initials: Y
authors.lastname: Liu
authors.forename: ChunYu
authors.initials: C
authors.lastname: Gong
authors.forename: XiuJui
authors.initials: X
authors.lastname: Du
authors.forename: YingJie
authors.initials: Y
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D020940:Reishi / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D011134:Polysaccharides