title: Synthesis, characterization, DNA-binding study and anticancer properties of ternary metal(II) complexes of edda and an intercalating ligand.
abstract: A series of ternary metal(ii) complexes {M(phen)(edda); 1a (Cu), 1b (Co), 1c (Zn), 1d (Ni); H(2)edda = N,N(')-ethylenediaminediacetic acid} of N,N'-ethylene-bridged diglycine and 1,10-phenanthroline were synthesized and characterized by elemental analysis, FTIR, UV-visible spectroscopy and magnetic susceptibility measurement. The interaction of these complexes with DNA was investigated using CD and EPR spectroscopy. MTT assay results of 1a-1c , screened on MCF-7 cancer cell lines, show that synergy between the metal and ligands results in significant enhancement of their antiproliferative properties. Preliminary results from apoptosis and cell cycle analyses with flow cytometry are reported. seems to be able to induce cell cycle arrest at G(0)/G(1). The crystal structure of 1a is also included.
authors.affiliation: Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300, Kuala Lumpur, Malaysia. ngch@mail.utar.edu.my
authors.forename: Chew Hee
authors.initials: CH
authors.lastname: Ng
authors.forename: King Chow
authors.initials: KC
authors.lastname: Kong
authors.forename: Sze Tin
authors.initials: ST
authors.lastname: Von
authors.forename: Pauline
authors.initials: P
authors.lastname: Balraj
authors.forename: Paul
authors.initials: P
authors.lastname: Jensen
authors.forename: Eswary
authors.initials: E
authors.lastname: Thirthagiri
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hamada
authors.forename: Makoto
authors.initials: M
authors.lastname: Chikira
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D008297:Male; D008670:Metals / Q000737:chemistry*; D012474:Salmon
chemical_list: D000970:Antineoplastic Agents; D007364:Intercalating Agents; D008024:Ligands; D008670:Metals; D004247:DNA