title: [The molecular mechanism of survivin expression in activated human peripheral lymphocytes].
abstract: OBJECTIVE
  Investigate the molecular mechanism of regulating survivin expression and related signal transduction pathway, molecular cascade reaction and biological effects in activated PBMC.
  
  
  METHODS
  The expression of survivin and related proteins were detected by Western blot in PBMC stimulated by PHA and rhIL-2 with or without JAK inhibitor-AG490 treatment, and FCM was performed to analyze cell cycle and cell division.
  
  
  RESULTS
  Our results indicated that molecular and cellular reactions in PBMC activated by PHA and rhIL-2 were dependent on time series. At first, the phosphorylation of Stat3 and Stat5 were observed, then, protein levels of CyclinD3 and CyclinE increased, and the stimulated PBMC began to enter to S phage with survivin protein expression was initiated, which at last resulted in cell division with dramatically increasing expression of survivin protein. AG490 could significantly inhibit all these reactions but had no effect on the expressions of the cell cycle inhibitor-P21 and anti-apoptosis protein-Bcl-2.
  
  
  CONCLUSIONS
  The expression of survivin in stimulated PBMC was dependent on the primarily activated JAK-STAT pathway, which upregulated CyclinD3 and CyclinE protein levels, initiated the cell cycle progression, and induced cell cycle-dependent survivin expression, and so survivin was involved in cell division and cell proliferation.
authors.affiliation: Institute of Radiation Medicine, Beijing 100850, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: Zhu-zhong
authors.initials: ZZ
authors.lastname: Mei
authors.forename: Jun-jie
authors.initials: JJ
authors.lastname: Qian
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.forename: Bao-lei
authors.initials: BL
authors.lastname: Tian
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Sun
mesh_terms: D015815:Cell Adhesion Molecules; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins; D050796:STAT3 Transcription Factor; D015398:Signal Transduction; D000077022:Survivin; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: C107489:BIRC5 protein, human; D015815:Cell Adhesion Molecules; C470661:ICAM-L protein, Leishmania gerbelli; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D015800:Protozoan Proteins; D050796:STAT3 Transcription Factor; D000077022:Survivin; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide