title: Aryl hydrocarbon receptor and estrogen receptor ligand activity of organic extracts from road dust and diesel exhaust particulates.
abstract: A wide variety of contaminants derived from diesel and gasoline engines, tire, asphalt, and natural organic compounds is found in road dust. Polycyclic aromatic compounds (PACs) are the important toxic targets among various contents in road dust and diesel exhaust particulates (DEPs), and endocrine-disrupting activity of PACs was suggested. In the present study, aryl hydrocarbon receptor (AhR) ligand activity was confirmed in the extract of both road dust and DEPs. In the separation of the extracts for both road dust and DEPs with reversed-phase HPLC, it was found that polar fractions contributed to significant AhR ligand activity in both a mouse hepatoma (H1L1) cell system and a yeast system. Furthermore, the contribution of these polar fractions was higher in DEPs than in road dust, probably because of the greater concentration of oxy-PAHs in DEPs than in road dust. The contribution of contaminants associated with the polar region to AhR ligand activity was also evident following the separation of road dust with normal-phase HPLC. Additionally, remarkable estrogen receptor (ER) ligand activity was detected in the highly polar region separated with normal-phase HPLC. It is suggested that many unknown AhR or ER ligand active compounds are contained in the polar region.
authors.affiliation: Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Misaki
authors.forename: Masato
authors.initials: M
authors.lastname: Suzuki
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Iida
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Kato
authors.forename: Saburo
authors.initials: S
authors.lastname: Matsui
authors.forename: Tomonari
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000393:Air Pollutants / Q000032:analysis* / Q000633:toxicity; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004391:Dust / Q000032:analysis; D007564:Japan; D008024:Ligands; D051379:Mice; D052638:Particulate Matter / Q000032:analysis* / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014407:Tumor Cells, Cultured; D001335:Vehicle Emissions / Q000032:analysis* / Q000633:toxicity
chemical_list: D000393:Air Pollutants; D004391:Dust; D008024:Ligands; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D011960:Receptors, Estrogen; D001335:Vehicle Emissions