Regulation of the type II secretion structural gene xpsE in Xanthomonas campestris Pathovar campestris by the global transcription regulator Clp.
GspE is an important component of the type II secretion system (T2SS) in Gram-negative bacteria that supplies energy for the process of protein secretion. The role of GspE and its interactions with other components have been intensively studied. However, regulation of the gspE gene is poorly understood. In this study, we demonstrated that the transcription of xpsE, a homologue of gspE in Xanthomonas campestris pathovar campestris (Xcc), is upregulated by Clp, a homologue of the cyclic AMP-receptor protein, by directly binding to the xpsE promoter region. Overexpression of the clp gene in Xcc wild-type strain 8004 enhanced the production of XpsE as well as endoglucanase and extracellular polysaccharide (EPS).
Ge
Chao
C
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China.
He
Chaozu
C
D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005110:Extracellular Space / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011134:Polysaccharides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D037521:Virulence Factors / Q000378:metabolism; D016959:Xanthomonas campestris / Q000235:genetics*
D001426:Bacterial Proteins; C436751:Clp protein, Xanthomonas campestris; D004268:DNA-Binding Proteins; D026901:Membrane Transport Proteins; D011134:Polysaccharides; D014157:Transcription Factors; D037521:Virulence Factors; C071567:general secretion pathway protein, Xanthomonas campestris