title: [p53 Codon 72 and 16-bp duplication polymorphisms of gastric cancer in Koreans].
abstract: OBJECTIVE
  p53 gene plays an important role in cell cycle control in response to DNA damage which may increase the probability of mutations leading to carcinogenesis. The role of p53 gene polymorphisms [codon 72 (exon 4) and 16-bp duplication (intron 3)] as potential markers indicating cancer risk remains inconclusive, and the data on gastric cancer are very limited. The aim of this study was to assess the role of p53 gene polymorphisms in the risk of gastric cancer and in the determination of genetic susceptibility to gastric cancer in Koreans.
  
  
  METHODS
  We analysed p53 genotypes using a polymerase chain reaction-based restriction fragment length polymorphism assay in a population-based case-control study in 120 gastric cancer patients and 145 cancer-free controls in Koreans.
  
  
  RESULTS
  There was no specific genotype of p53 gene polymorphism in the gastric cancer patients compared to cancer-free controls. In p53 codon 72 and 16-bp duplication polymorphisms, the frequency and distribution of genotypes showed no statistical significance (p=0.7125 and p=0.1659). There was no difference in genotype by histologic subtypes, location of lesion, and age. However, the genotypic distribution in the patient subgroups with a history of heavy cigarette smoking of p53 16-bp duplication polymorphism were significantly different from those of cancer-free controls (p=0.0079).
  
  
  CONCLUSIONS
  The p53 codon 72 and 16-bp duplication polymorphisms were not associated with the increased risk of gastric cancer and did not seem to contribute to gastric cancer susceptibility among Koreans. It is possible that p53 16-bp duplication polymorphism modulates the risk of smoking-induced gastric cancer development in Koreans. In order to clarify the associations between specific genotypes and gastric cancer risk, the evaluations of these polymorphisms in other ethnic backgrounds with larger number of patients would be needed.
authors.affiliation: Department of Internal Medicine, Hanyang University College of Medicine, Seoul, Korea.
authors.forename: Jung Mi
authors.initials: JM
authors.lastname: Kim
authors.forename: Oh Young
authors.initials: OY
authors.lastname: Lee
authors.forename: Chun Geun
authors.initials: CG
authors.lastname: Lee
authors.forename: Sung Joon
authors.initials: SJ
authors.lastname: Kwon
authors.forename: Kyung Suk
authors.initials: KS
authors.lastname: Kim
authors.forename: Won
authors.initials: W
authors.lastname: Moon
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Koh
authors.forename: Hang Lak
authors.initials: HL
authors.lastname: Lee
authors.forename: Byoeng Chul
authors.initials: BC
authors.lastname: Yoon
authors.forename: Ho Soon
authors.initials: HS
authors.lastname: Choi
authors.forename: Joon Soo
authors.initials: JS
authors.lastname: Hahm
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Lee
authors.forename: Dong Hoo
authors.initials: DH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003062:Codon; D003627:Data Interpretation, Statistical; D005260:Female; D016158:Genes, p53; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007723:Korea; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D003062:Codon