Incorporation of the HERG potassium channel in a mercury supported lipid bilayer.
The HERG potassium channel was incorporated in a mercury-supported tethered bilayer lipid membrane (tBLM) obtained by anchoring a thiolipid monolayer to the mercury surface and by self-assembling a lipid monolayer on top of it from a lipid film spread on the surface of an electrolyte solution. HERG was then incorporated in this tBLM from its micellar solution in Triton X-100, thus avoiding the use of vesicles in the preparation of the tBLM and of proteoliposomes in channel incorporation. The HERG "inward" current following a repolarization step was obtained by subtracting the current recorded upon addition of the specific inhibitor WAY from that recorded prior to this addition. This current was compared with that reported in the literature by the patch-clamp technique.
Becucci
Lucia
L
Dipartimento di Chimica, Via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy. lucia.becucci@unifi.it
Carbone
Maria V
MV
Biagiotti
Tiziana
T
D'Amico
Massimo
M
Olivotto
Massimo
M
Guidelli
Rolando
R
D002460:Cell Line; D051638:Ether-A-Go-Go Potassium Channels / Q000737:chemistry*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008628:Mercury / Q000737:chemistry*; D015394:Molecular Structure
D051638:Ether-A-Go-Go Potassium Channels; D008051:Lipid Bilayers; D008628:Mercury