Anemia in beta-thalassemia patients targets hepatic hepcidin transcript levels independently of iron metabolism genes controlling hepcidin expression.
Thalassemia associates anemia and iron overload, two opposite stimuli regulating hepcidin gene expression. We characterized hepatic hepcidin expression in 10 thalassemia major and 13 thalassemia intermedia patients. Hepcidin mRNA levels were decreased in the thalassemia intermedia group which presented both lower hemoglobin and higher plasma soluble transferrin receptor levels. There was no relationship between hepcidin mRNA levels and those of genes controlling iron metabolism, including HFE, hemojuvelin, transferrin receptor-2 and ferroportin. These results underline the role of erythropoietic activity on hepcidin decrease in thalassemic patients and suggest that mRNA modulations of other studied genes do not have a significant impact.
Camberlein
Emilie
E
Inserm U-522, IFR 140, University Hospital Pontchaillou, Rennes, France.
Zanninelli
Giuliana
G
Détivaud
Lénaïck
L
Lizzi
Anna Rita
AR
Sorrentino
Francesco
F
Vacquer
Stefania
S
Troadec
Marie-Bérengère
MB
Angelucci
Emanuele
E
Abgueguen
Emmanuelle
E
Loréal
Olivier
O
Cianciulli
Paolo
P
Lai
Maria Eliana
ME
Brissot
Pierre
P
D000328:Adult; D000368:Aged; D000740:Anemia / Q000150:complications*; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis*; D027682:Cation Transport Proteins / Q000096:biosynthesis; D005260:Female; D058851:GPI-Linked Proteins; D005786:Gene Expression Regulation; D000071020:Hemochromatosis Protein; D064451:Hepcidins; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis; D008875:Middle Aged; D011990:Receptors, Transferrin / Q000096:biosynthesis; D017086:beta-Thalassemia / Q000150:complications*; D000098742:Ferroportin
D023181:Antimicrobial Cationic Peptides; D027682:Cation Transport Proteins; D058851:GPI-Linked Proteins; C578702:HAMP protein, human; C103673:HFE protein, human; C481006:HJV protein, human; D000071020:Hemochromatosis Protein; D064451:Hepcidins; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D011990:Receptors, Transferrin; C503640:TFR2 protein, human; D000098742:Ferroportin; D007501:Iron