Mesosome formation is accompanied by hydrogen peroxide accumulation in bacteria during the rifampicin effect.
Ultrastructural alteration and hydrogen peroxide localization were examined in Xanthomonas campestris pv. phaseoli during rifampicin effect using transmission electron microscopy. Bacterial cells were treated with rifampicin and then were examined by electron microscopy to observe the changes of ultrastructure or hydrogen peroxide accumulation in living cells that took place before lysis. Intriguingly, rifampicin treatment led to presence of an additional location of hydrogen peroxide accumulation within the cells. There was an association between the frequency and size of the additional location of hydrogen peroxide accumulation and the concentration of rifampicin. Furthermore, an additional ultrastructure, mesosomes, was also present in cells during rifampicin effect. The frequency and size of mesosome increased with the increasing concentration of rifampicin. Result of multiple linear regression showed that the size of mesosome plays as a key factor in the quantity of excess hydrogen peroxide accumulation in cells during rifampicin effect. Linear correlation was confirmed between quantity of excess hydrogen peroxide accumulation and the size of mesosome in cells during rifampicin effect. This finding intensely indicated that mesosomes are just the additional location of hydrogen peroxide accumulation in cells under cellular injury caused by rifampicin treatment. The mesosome formation is always accompanied by excess hydrogen peroxide accumulation in X. campestris pv. phaseoli during rifampicin effect.
Li
Xin
X
MOE Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
Feng
Hanqing Q
HQ
Pang
Xinyue Y
XY
Li
Hongyu Y
HY
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology*; D016959:Xanthomonas campestris / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*
D000904:Antibiotics, Antitubercular; D016877:Oxidants; D006861:Hydrogen Peroxide; D012293:Rifampin