title: Self-assembled water-soluble nucleic acid probe tiles for label-free RNA hybridization assays.
abstract: The DNA origami method, in which long, single-stranded DNA segments are folded into shapes by short staple segments, was used to create nucleic acid probe tiles that are molecular analogs of macroscopic DNA chips. One hundred trillion probe tiles were fabricated in one step and bear pairs of 20-nucleotide-long single-stranded DNA segments that act as probe sequences. These tiles can hybridize to their targets in solution and, after adsorption onto mica surfaces, can be examined by atomic force microscopy in order to quantify binding events, because the probe segments greatly increase in stiffness upon hybridization. The nucleic acid probe tiles have been used to study position-dependent hybridization on the nanoscale and have also been used for label-free detection of RNA.
authors.affiliation: Center for Single Molecule Biophysics, Arizona State University, Tempe, AZ 85287, USA.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Ke
authors.forename: Stuart
authors.initials: S
authors.lastname: Lindsay
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
mesh_terms: D000199:Actins / Q000235:genetics; D000327:Adsorption; D000538:Aluminum Silicates; D000818:Animals; D002460:Cell Line; D004277:DNA, Single-Stranded; D020869:Gene Expression Profiling / Q000379:methods*; D016654:Genes, RAG-1; D016259:Genes, myc; D051379:Mice; D018625:Microscopy, Atomic Force; D015336:Molecular Probe Techniques; D049329:Nanostructures; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes; D012313:RNA / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D012995:Solubility
chemical_list: D000199:Actins; D000538:Aluminum Silicates; D004277:DNA, Single-Stranded; D015345:Oligonucleotide Probes; D012313:RNA; C011934:mica