Sphingomonas insulae sp. nov., isolated from soil.
A Gram-negative, motile and rod-shaped Sphingomonas-like bacterial strain, DS-28(T), was isolated from the soil of Dokdo, Korea, and was subjected to a polyphasic taxonomic study. It grew optimally at around pH 6.5 and 25 degrees C in the presence of 0-0.5% NaCl. It contained Q-10 as the predominant ubiquinone and C(18:1)omega7c, C(14:0) 2-OH and C(16:0) as the major fatty acids. Major polar lipids were sphingoglycolipid, phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 67.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-28(T) belonged to the genus Sphingomonas. Levels of 16S rRNA gene sequence similarity between strain DS-28(T) and the type strains of Sphingomonas species were in the range 93.3-98.3%. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DS-28(T) is distinguishable from recognized Sphingomonas species. On the basis of phenotypic, phylogenetic and genetic data, strain DS-28(T) represents a novel species of the genus Sphingomonas, for which the name Sphingomonas insulae sp. nov. is proposed. The type strain is DS-28(T) (=KCTC 12872(T)=JCM 14603(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.re.kr
Kang
So-Jung
SJ
Lee
Soo-Young
SY
Oh
Tae-Kwang
TK
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S