Determination of cytotoxicity of nephrotoxins on murine and human kidney cell lines.
The present study investigates renal inner medullary collecting duct (mIMCD3) cells and human embryonic kidney cells (HEK293) for evaluation of cytotoxicity of nephrotoxic compounds. The 24 h LC(50) values for cisplatin, paraquat and ibuprofen in mIMCD3 cells were 135, 155 and 3600 microM, respectively. The 24 h LC(50) values for paraquat and ibuprofen in HEK293 cells were 180 and 1000 microM, respectively. Effects of hyperosmolality on cytotoxicity of paraquat were additive in mIMCD3 cells. These data demonstrate that renal hyperosmolality has an additive effect on cytoxicity of paraquat.
Park
Eun-Kee
EK
Department of Entomology and Cancer Research Center, University of California-Davis, CA, USA. eunkee.park@ddb.nsw.gov.au
Mak
Sally K
SK
Kültz
Dietmar
D
Hammock
Bruce D
BD
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002945:Cisplatin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007052:Ibuprofen / Q000633:toxicity*; D007668:Kidney / Q000166:cytology* / Q000187:drug effects*; D007679:Kidney Medulla / Q000166:cytology / Q000187:drug effects; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000187:drug effects; D007928:Lethal Dose 50; D051379:Mice; D009994:Osmolar Concentration; D010269:Paraquat / Q000633:toxicity*
D010269:Paraquat; D002945:Cisplatin; D007052:Ibuprofen