title: Association of transient receptor potential canonical type 3 (TRPC3) channel transcripts with proinflammatory cytokines.
abstract: We investigated whether expression of non-selective cation channels of the transient receptor potential canonical (TRPC) channel family are associated with proinflammatory cytokines in monocytes. Using quantitative RT-PCR we studied the expression of TRPC3, interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) in monocytes from 15 patients with essential hypertension and 16 age- and sex-matched normotensive control subjects. We observed an approximately 8-fold increase of TRPC3 transcripts in monocytes from patients with essential hypertension compared to normotensive control subjects (p<0.05). We found an approximately 3-fold increase of IL-1beta, and an approximately 9-fold increase of TNF-alpha in patients with essential hypertension compared to normotensive control subjects (each p<0.05). We observed a significant correlation between TRPC3 transcripts with systolic blood pressure, expression of IL-1beta, and TNF-alpha. Using quantitative RT-PCR we observed an association of TRPC3 transcripts and proinflammatory cytokines in monocytes.
authors.affiliation: Charité Campus Benjamin Franklin, Med. Klinik Nephrologie, Hindenburgdamm 30, 12200 Berlin, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Thilo
authors.forename: Alexandra
authors.initials: A
authors.lastname: Scholze
authors.forename: Dao Yan
authors.initials: DY
authors.lastname: Liu
authors.forename: Walter
authors.initials: W
authors.lastname: Zidek
authors.forename: Martin
authors.initials: M
authors.lastname: Tepel
mesh_terms: D000368:Aged; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000235:genetics / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D050052:TRPC Cation Channels / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D012333:RNA, Messenger; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D014409:Tumor Necrosis Factor-alpha