Heat-shock response in Arabidopsis thaliana explored by multiplexed quantitative proteomics using differential metabolic labeling.
We have developed a general method for multiplexed quantitative proteomics using differential metabolic stable isotope labeling and mass spectrometry. The method was successfully used to study the dynamics of heat-shock response in Arabidopsis thaliana. A number of known heat-shock proteins were confirmed, and some proteins not previously associated with heat shock were discovered. The method is applicable in stable isotope labeling and allows for high degrees of multiplexing.
Palmblad
Magnus
M
The BioCentre, The University of Reading, Whiteknights, Post Office Box 221, Reading RG6 6AS, United Kingdom. n.m.palmblad@lumc.nl
Mills
Davinia J
DJ
Bindschedler
Laurence V
LV
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002853:Chromatography, Liquid; D018869:Heat-Shock Response / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D020543:Proteome / Q000378:metabolism* / Q000502:physiology; D040901:Proteomics; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry
D029681:Arabidopsis Proteins; D009587:Nitrogen Isotopes; D020543:Proteome