title: Comparative examination of anti-proliferative activities of (-)-epigallocatechin gallate and (--)-epigallocatechin against HCT116 colorectal carcinoma cells.
abstract: We compared anti-proliferative activities of (-)-epigallocatechin gallate (EGCG) and (-)-epigallocatechin (EGC) against HCT116 colorectal carcinoma cells. These catechins inhibited cell growth to nearly the same extent at low cell confluency in plates. However, their inhibitory effect grew weaker as cell confluence increased, and this tendency was more conspicuous for EGC than for EGCG. Both EGCG and EGC activated the phosphorylation of the major MAPKs, ERK, JNK, and p38, in the HCT116 cells as in many other established human cancer cells though to different extents. Cell cycle analyses, DNA fragmentation assays, and TUNEL assays as well as Western blot assays suggested that these catechins inhibited cell growth through mitogen-activated protein kinase (MAPK)-mediated apoptosis rather than cell cycle regulation.
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Chemistry, Material and Bioengineering, Kansai University, Suita, Osaka 564-8680, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inaba
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Nagaoka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kushima
authors.forename: Ayako
authors.initials: A
authors.lastname: Kumagai
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Minoru
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: Kimiye
authors.initials: K
authors.lastname: Baba
authors.forename: Shinichi
authors.initials: S
authors.lastname: Uesato
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045325:HCT116 Cells; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D002392:Catechin; C045651:epigallocatechin gallate; D020928:Mitogen-Activated Protein Kinases; D053148:Caspase 3; C057580:gallocatechol