Preparation of konjac glucomannan hydrogels as DNA-controlled release matrix.
In this study, hydrogels for DNA-controlled release was prepared with konjac glucomannan (KGM), a water-soluble non-ionic polysaccharide, by means of deacetylated reaction and physically cross-linking method under mild conditions. The properties of the KGM hydrogels were analyzed by FTIR spectra and scanning electron microscopy (SEM). The integrality of the released DNA was investigated by circular dichroism (CD). The DNA release kinetics was performed using the DNA-loaded KGM gels in buffer solutions of pH 7.4 at 37+/-0.5 degrees C. Peppas model and Higuchi model were used to analysis the DNA release mechanism; the data indicated that the DNA release can be controlled by changing the preparation conditions and the structure parameters of the gels. This study suggested that the KGM hydrogels have a potential use for advanced controlled release.
Wen
Xian
X
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610065, PR China.
Wang
Ting
T
Wang
Zhiyong
Z
Li
Li
L
Zhao
Changsheng
C
D031056:Amorphophallus / Q000737:chemistry*; D000818:Animals; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D008956:Models, Chemical; D012474:Salmon
D003692:Delayed-Action Preparations; D020100:Hydrogels; D008351:Mannans; C022901:(1-6)-alpha-glucomannan; D004247:DNA