Toxicity of imidacloprid to the terrestrial isopod Porcellio scaber (Isopoda, Crustacea).
Imidacloprid is a neonicotinoid insecticide with neurotoxic action that, as a possible alternative for commonly used organophosphorus pesticides, has gained registration in about 120 countries for use in over 140 agricultural crops. Only few data are available on its toxicity for soil invertebrates. We therefore assessed the effects of imidacloprid on survival, weight gain, feeding rate, total protein content, glutathione S-transferase activity (GST), and digestive gland epithelial thickness in juveniles and adults of the terrestrial isopod Porcellio scaber. After two weeks of feeding on imidacloprid-dosed food, weight gain (NOEC 5 microg/g dry food) and feeding rate (NOEC 10 microg/g) in juveniles, and feeding rate (NOEC<10 microg/g) and digestive gland epithelial thickness (NOEC<10 microg/g) in adults were most affected. In juveniles induction of GST activity and increase of total protein content per wet animal weight was detected at 5 microg/g dry food, whereas in adults a reduction of GST was observed at 25 microg/g (NOEC 10 microg/g). An estimate of actual intake rates suggests that imidacloprid affects isopods at similar exposure concentrations as insects. The toxicity of imidacloprid was similar to that of the organophosphorus pesticide diazinon, tested earlier using the same methods [Stanek, K., Drobne, D., Trebse, P., 2006. Linkage of biomarkers along levels of biological complexity in juvenile and adult diazinon fed terrestrial isopod (Porcellio scaber, Isopoda, Crustacea). Chemosphere 64, 1745-1752]. At actual environmental concentrations, diazinon poses a higher risk to P. scaber. Due to its increasing use in crop protection and higher persistence in soil, imidacloprid might however, be potentially more dangerous after long-term application. We conclude that toxicity testing with P. scaber provides relevant, repeatable, reproducible and comparable toxicity data that is useful for the risk assessment of pesticides in the terrestrial environment.
Drobne
Damjana
D
University of Ljubljana, Biotechnical Faculty, Department of Biology, Vecna Pot 111, SI-1000 Ljubljana, Slovenia. Damjana.Drobne@bf.uni-lj.si <Damjana.Drobne@bf.uni-lj.si>
Blazic
Mateja
M
Van Gestel
Cornelis A M
CA
Leser
Vladka
V
Zidar
Primoz
P
Jemec
Anita
A
Trebse
Polonca
P
D000821:Animal Feed; D000818:Animals; D004064:Digestive System / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism; D043143:Hepatopancreas / Q000187:drug effects; D007093:Imidazoles / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D033321:Isopoda / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007928:Lethal Dose 50; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D019055:No-Observed-Adverse-Effect Level; D018570:Risk Assessment; D012989:Soil Pollutants / Q000633:toxicity*; D018675:Toxicity Tests
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D012989:Soil Pollutants; C082359:imidacloprid; D005982:Glutathione Transferase