title: Proteomic analysis of dog tears for potential cancer markers.
abstract: The first reference map of the proteome of pooled normal dog tears was created using 2-dimensional polyacrylamide gel electrophoresis and the identity of a number of the major species determined using matrix-assisted laser desorption time of flight mass spectrometry (MALDI-TOF) and peptide mass fingerprint matching on protein sequence databases. In order to understand the changes in protein expression in the tear film of dogs with cancer, tears from such animals were similarly examined. A number of differences were found between the tears of healthy dogs and the dogs with cancer. Differences were found in levels of actin and albumin and in an unidentified protein which may be analogous to human lacryglobulin. These findings suggest that it may be possible to develop tear film analysis to provide a simple non-invasive test for the diagnosis and/or management of canine cancers.
authors.affiliation: Institute for Eye Research, Rupert Myers Building, The University of New South Wales, Kensington, NSW 2052, Australia.
authors.forename: Carla
authors.initials: C
authors.lastname: de Freitas Campos
authors.forename: Nerida
authors.initials: N
authors.lastname: Cole
authors.forename: Derek
authors.initials: D
authors.lastname: Van Dyk
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Walsh
authors.forename: Penelope
authors.initials: P
authors.lastname: Diakos
authors.forename: Denise
authors.initials: D
authors.lastname: Almeida
authors.forename: Adriana
authors.initials: A
authors.lastname: Torrecilhas
authors.forename: José Luiz
authors.initials: JL
authors.lastname: Laus
authors.forename: Mark D P
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D000199:Actins / Q000032:analysis; D000418:Albumins / Q000032:analysis; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D016022:Case-Control Studies; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D005914:Globins / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009185:Myelin Proteins; D009369:Neoplasms / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D011510:Proteolipids; D040901:Proteomics; D060765:Secretoglobins; D013666:Tears / Q000737:chemistry*; D014598:Uteroglobin
chemical_list: D000199:Actins; D000418:Albumins; D015415:Biomarkers; D009185:Myelin Proteins; D011510:Proteolipids; D060765:Secretoglobins; D005914:Globins; D014598:Uteroglobin