Strategy for in situ detection of natural transformation-based horizontal gene transfer events.
A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.
Rizzi
Aurora
A
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy.
Pontiroli
Alessandra
A
Brusetti
Lorenzo
L
Borin
Sara
S
Sorlini
Claudia
C
Abruzzese
Alessandro
A
Sacchi
Gian Attilio
GA
Vogel
Timothy M
TM
Simonet
Pascal
P
Bazzicalupo
Marco
M
Nielsen
Kaare Magne
KM
Monier
Jean-Michel
JM
Daffonchio
Daniele
D
D000150:Acinetobacter / Q000235:genetics*; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D014169:Transformation, Bacterial / Q000235:genetics*
D049452:Green Fluorescent Proteins