title: Increased bronchial NO output in severe atopic eczema in children and adolescents.
abstract: Atopic children have an increased risk for asthma, which is preceded by bronchial inflammation. Exhaled nitric oxide (NO) measured at multiple exhalation flow rates can be used to assess alveolar NO concentration and bronchial NO flux, which reflect inflammation in lung periphery and central airways, respectively. Exhaled breath condensate is another non-invasive method to measure lung inflammation. The purpose of the present study was to find out if the severity of atopic eczema is associated with lung inflammation that can be observed with these non-invasive tests. We studied 81 patients (7-22 yr old) with atopic eczema and increased wheat-specific IgE (>or=0.4 kUA/l) and no diagnosis of asthma. Exhaled NO was measured at multiple exhalation flow rates, and bronchial NO flux and alveolar NO concentration were calculated. Cysteinyl-leukotriene concentrations were measured in exhaled breath condensate. The patients were divided into two groups according to the severity of atopic eczema. Patients with severe atopic eczema had enhanced bronchial NO output as compared with patients with mild eczema (2.1 +/- 0.5 vs. 0.9 +/- 0.1, p = 0.003). No statistically significant differences in alveolar NO concentrations were found between the groups. In the whole group of patients, the bronchial NO output correlated positively with serum eosinophil protein X (r(s) = 0.450, p < 0.001), serum eosinophil cationic protein (r(s) = 0.393, p < 0.001), serum total IgE (r(s) = 0.268, p = 0.016) and with urine eosinophil protein X (r(s) = 0.279, p = 0.012), but not with lung function. Alveolar NO concentration correlated positively with serum eosinophil protein X (r(s) = 0.444, p < 0.001) and with serum eosinophil cationic protein (r(s) = 0.362, p = 0.001). Measurable cysteinyl-leukotriene concentrations in exhaled breath condensate were found only in one-third of the patients, and there were no differences between the two groups. The results show that increased bronchial NO output is associated with eosinophilic inflammation and severe atopic eczema in patients without established asthma.
authors.affiliation: The Immunopharmacology Research Group, Medical School, University of Tempere and Research Unit, Tempere University Hospital, Tempere, Finland.
authors.forename: Laura
authors.initials: L
authors.lastname: Linkosalo
authors.forename: Lauri
authors.initials: L
authors.lastname: Lehtimäki
authors.forename: Jukka
authors.initials: J
authors.lastname: Laitinen
authors.forename: Minna
authors.initials: M
authors.lastname: Kaila
authors.forename: Kati
authors.initials: K
authors.lastname: Holm
authors.forename: Eeva
authors.initials: E
authors.lastname: Moilanen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000503:physiopathology / Q000652:urine; D002648:Child; D003545:Cysteine / Q000737:chemistry; D003876:Dermatitis, Atopic / Q000097:blood / Q000276:immunology* / Q000503:physiopathology / Q000652:urine; D047150:Eosinophil Cationic Protein / Q000652:urine; D047208:Eosinophil-Derived Neurotoxin / Q000652:urine; D045853:Exhalation; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D015289:Leukotrienes / Q000737:chemistry; D008297:Male; D009569:Nitric Oxide / Q000737:chemistry / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000378:metabolism; D012720:Severity of Illness Index; D012882:Skin Tests; D013147:Spirometry; D013223:Statistics as Topic
chemical_list: D015289:Leukotrienes; C112381:cysteinyl-leukotriene; D009569:Nitric Oxide; D007073:Immunoglobulin E; D047208:Eosinophil-Derived Neurotoxin; D047150:Eosinophil Cationic Protein; D003545:Cysteine