Microdialysis technique as a monitoring system for acute complications of diabetes.
The objective of the study was to establish the quasi-continuous courses, using microdialysis technique, of glucose, lactate, and glycerol concentrations in interstitial fluid of abdominal adipose tissue during the standard treatment of acute diabetes complications. Clinical studies were carried out on 31 diabetic patients during the initial 48 h of the treatment. In all but two obese female patients with hyperglycemic hyperosmolar state (HHS) did glucose concentration in perfusion fluid (PF) reflect concentration in capillary blood. The recovery of glucose correlated with patients' body mass index (r = 0.55). It was significantly higher in lean and overweight patients (91 +/- 15%) than in obese patients (55 +/- 31%). The course of lactate concentration in PF coincided with the course in venous blood (2.1 +/- 0.3 mmol/L vs. 2.0 +/- 0.5 mmol/L, P = 0.35). Glycerol concentration was 267 +/- 41 micromol/L and 133 +/- 40 micromol/L in PF and venous blood, respectively (P = 0.004). The study indicated that microdialysis may be an effective tool to monitor concentration of different metabolites in interstitial fluid of the adipose tissue during treatment of the acute complications of diabetes. Applicability of the technique in the monitoring of HHS, especially in obese female patients, needs further investigation.
Ciechanowska
Anna
A
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland. Anna.Ciechanowska@ibib.waw.pl
Ladyzynski
Piotr
P
Wojcicki
Jan M
JM
Sabalinska
Stanislawa
S
Krzymien
Janusz
J
Pulawska
Elzbieta
E
Karnafel
Waldemar
W
Foltynski
Piotr
P
Kawiak
Jerzy
J
D050153:Abdominal Fat / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003922:Diabetes Mellitus, Type 1 / Q000150:complications*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D045604:Extracellular Fluid / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D006943:Hyperglycemia / Q000209:etiology / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D017551:Microdialysis; D008875:Middle Aged
D019344:Lactic Acid; D005947:Glucose; D005990:Glycerol