title: [A new bromine-containing reagent for cysteine modification].
abstract: 5-Bromo-2[(2-iodoacetyl)amino]benzenesulfonic acid (AIBSA), a reagent for modification of free of cysteine thiol groups in proteins and peptides, was synthesized. Rate constants of its interaction with thiol groups were determined. The presence of a bromine atom allows an easy identification of the AIBSA-labeled peptides in mass spectra due to the characteristic isotope distribution. The compound is stable in solution and under exposure to light.
authors.forename: T V
authors.initials: TV
authors.lastname: Minaeva
authors.forename: E N
authors.initials: EN
authors.lastname: Danilovtseva
authors.forename: V V
authors.initials: VV
authors.lastname: Annenkov
authors.forename: A V
authors.initials: AV
authors.lastname: Novikov
authors.forename: A L
authors.initials: AL
authors.lastname: Vereshchagin
authors.forename: M A
authors.initials: MA
authors.lastname: Grachev
mesh_terms: D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects; D001966:Bromine / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D007460:Iodoacetamide / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D011506:Proteins / Q000737:chemistry*
chemical_list: C526703:5-bromo-2-((2-iodoacetyl)amino)benzenesulfonic acid; D001557:Benzenesulfonates; D011506:Proteins; D003545:Cysteine; D001966:Bromine; D007460:Iodoacetamide