Alternatively spliced isoforms encoded by cadherin genes from C. elegansgenome.
Cadherins are calcium-dependent, homophilic, cell-cell adhesion receptors that regulate morphogenesis, pattern formation and cell migration. The C. elegans Genome Sequencing Consortium has reported 12 genes from C. elegansgenome encoding members of the cadherin superfamily. Alternative splicing of eukaryotic pre-mRNAs is a mechanism for generating potentially many transcript isoforms from a single gene. Here, using a combination of various gene or exon finding programmes and several other bioinformatics tools followed by experimental validation using RT-PCR, we have studied alternative splicing pattern in the cadherin encoding genes from C. elegansgenome. We have predicted that 7 of the 12 genes encoding the cadherin superfamily undergo extensive alternative splicing and encode for 12 new unreported alternatively spliced transcripts. Most of the alternatively spliced exons were found to be present at the 5' end of genes. These new previously un-detected spliced variants in C. eleganscadherin superfamily of genes could play vital roles in explaining the way cadherins act to control the processes like cell adhesion and morphogenesis.
Kashyap
Luv
L
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.
Tabish
Mohammad
M