title: [Comparative research of the influence factors of DNA extraction of bloodstain on the filter paper with Chelex-100 method].
abstract: OBJECTIVE
  To improve DNA extraction from bloodstain on the filter paper and to establish a rapid, simple, and cost-effective method for DNA extraction suitable for database construction.
  
  
  METHODS
  Seven hundred and fifty two aged bloodstains on filter paper were randomly divided into four groups. The four different DNA extraction methods were compared with each other, and two DNA extraction methods used for 63 fresh bloodstains on filter paper were also compared with each other.
  
  
  RESULTS
  There were no statistically significant differences observed among the four DNA extraction methods (P > 0.05) for aged bloodstains on filter paper; But the difference between the two DNA extraction methods for fresh bloodstains on filter paper was obviously (P < 0.05).
  
  
  CONCLUSIONS
  Extraction of DNA samples from aged bloodstains on filter paper can be accomplished by using Chlex-100 methodology directly with no need to wash the bloodstains.
authors.affiliation: Changzhou Public Security Bureau, Changzhou 213003, China. bahuajie@163.com
authors.forename: Hua-Jie
authors.initials: HJ
authors.lastname: Ba
authors.forename: Bing-quan
authors.initials: BQ
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Zhu
authors.forename: Zi-qing
authors.initials: ZQ
authors.lastname: Lin
mesh_terms: D001801:Blood Stains; D002614:Chelating Agents; D004247:DNA / Q000302:isolation & purification*; D019286:Endopeptidase K; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012117:Resins, Synthetic; D013048:Specimen Handling / Q000379:methods*; D014867:Water
chemical_list: D002614:Chelating Agents; D007202:Indicators and Reagents; D012117:Resins, Synthetic; D014867:Water; C024997:Chelex 100; D004247:DNA; D019286:Endopeptidase K