A Legionella pneumophila peptidyl-prolyl cis-trans isomerase present in culture supernatants is necessary for optimal growth at low temperatures.
Several Legionella pneumophila proteins were highly expressed in low-temperature supernatants. One of these proteins was the peptidyl-prolyl isomerase PpiB. Mutants lacking ppiB exhibited reduced growth at 17 degrees C. Since PpiB lacked a signal sequence and was present in 17 degrees C supernatants of type II and type IV secretion mutants, this protein may be secreted by a novel mechanism.
Söderberg
Maria A
MA
Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, IL 60611, USA.
Cianciotto
Nicholas P
NP
D003470:Culture Media / Q000737:chemistry*; D017931:DNA Primers / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D016952:Legionella pneumophila / Q000201:enzymology* / Q000254:growth & development*; D013058:Mass Spectrometry; D016296:Mutagenesis; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D017931:DNA Primers; D019696:Peptidylprolyl Isomerase