Effect of a topical treatment in organotypic culture of human breast skin after exposure to gamma-rays.
The early radiation of epidermal reactions can lead to healing of the lesion or radiation necrosis. There is no general agreement for either the prevention and/or treatment of radiation skin response, also as little is known about the immediate phases of this phenomenon. We investigated the early effects exerted by Healing and Wound Emulsion (HWE) on human skin response after ionizing radiation. Epidermal morphology, Heat Shock Protein (HSP) 70, and Transforming Growth Factor-beta1 (TGF-beta1) gene expression were investigated in organotypic human skin cultures undergoing a double dose of gamma-rays (2 Gy). HSP70 gene expression tended to be induced in the HWE group 6 hours after cream administration and was significantly up-regulated after 48 hours, when epidermal morphological alterations were evident. TGF-beta1 seems not affected in cream treated samples. HWE may stimulate skin to mount an early defensive response against damage induced by gamma rays.
Gagliano
Nicoletta
N
Department of Human Morphology, Extracellular Matrix Laboratory, University of Milan, Via Fratelli Cervi, 93 20090 Segrate, Milan, Italy. nicoletta.gagliano@unimi.it
Bedoni
M
M
Mantovani
G
G
Chiriva-Internati
M
M
Castelli
D
D
Torri
C
C
Donetti
E
E
D000328:Adult; D001940:Breast; D049109:Cell Proliferation / Q000528:radiation effects; D004655:Emulsions / Q000494:pharmacology*; D004817:Epidermis / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D009336:Necrosis; D009924:Organ Culture Techniques; D011855:Radiodermatitis / Q000517:prevention & control*; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D015854:Up-Regulation
D004655:Emulsions; D050884:HSP72 Heat-Shock Proteins; D053773:Transforming Growth Factor beta1