Caveolin-1 deficiency alters plasma lipid and lipoprotein profiles in mice.
Caveolae are specialized membrane microdomains formed as the result of local accumulation of cholesterol, glycosphingolipids, and the structural protein caveolin-1 (Cav-1). To further elucidate the role of Cav-1 in lipid homeostasis in-vivo, we analyzed fasting and post-prandial plasma from Cav-1 deficient mice on low or on high fat diet. In total plasma analysis, an increase in ceramide and hexosylceramide was observed. In cholesteryl ester (CE), we found an increased saturated+monounsaturated/polyunsaturated fatty acid ratio in fasting plasma of low fat fed Cav-1(-/-) mice with increased proportions of CE16:1, CE18:1, CE20:3, and decreased proportions of CE18:2 and CE22:6. Under high fat diet HDL-CE, free cholesterol and pre-beta-HDL were increased accompanied by a shift from slow to fast migrating alpha-HDL and expansion of apoE containing HDL. Our results demonstrate a significant role of Cav-1 in HDL-cholesterol metabolism and may reflect a variety of Cav-1 functions including modulation of ACAT activity and SR-BI function.
Heimerl
Susanne
S
Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93042 Regensburg, Germany.
Liebisch
Gerhard
G
Le Lay
Soazig
S
Böttcher
Alfred
A
Wiesner
Philipp
P
Lindtner
Silke
S
Kurzchalia
Teymuras V
TV
Simons
Kai
K
Schmitz
Gerd
G
D000818:Animals; D051242:Caveolin 1 / Q000172:deficiency / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D005215:Fasting / Q000378:metabolism*; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019518:Postprandial Period / Q000502:physiology*
D051242:Caveolin 1; D004041:Dietary Fats; D008055:Lipids; D008074:Lipoproteins