[Effects of Forsythia suspense extract on cell proliferation and activation of the mouse T lymphocytes in vitro].
OBJECTIVE
To investigate the effect of Forsythia suspensa (FS) extract on the activation and proliferation of the mouse T lymphocytes in vitro as well as its immunosuppressive effect.


METHODS
The lymphocytes were isolated from the lymphoid nodes of BALB/c mice. Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69, CD25 and CD 71 of the activated T lymphocytes in vitro in response to Concanavalin A (ConA). Stained with CFDA-SE, the mouse lymphocytes were stimulated by ConA. The effect of FS extract on cell proliferation in vitro was analyzed by flow cytometry.


RESULTS
FS extract decreased the expression of CD69, CD25 and CD71 at the final concentration of 40, 80 and 160 mg/L, respectively(P<0.05). CFDA-SE staining showed that FS extract at different concentration significantly inhibited the proliferation of lymphocytes induced by ConA (P<0.05).


CONCLUSIONS
FS extract can inhibit the activation and proliferation of T lymphocytes in response to ConA.
Yin
Le-le
LL
Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. raku1982@sina.com
Zeng
Yao-ying
YY
Huang
Xiu-yan
XY
Hou
Hui-na
HN
D000818:Animals; D002135:Calcium-Binding Proteins / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D031657:Forsythia / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008565:Membrane Proteins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D000072099:Serrate-Jagged Proteins
D002135:Calcium-Binding Proteins; D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins