A novel method to prepare water-dispersible magnetic nanoparticles and their biomedical applications: magnetic capture probe and specific cellular uptake.
A novel and simple method to form water-dispersed magnetic nanoparticles was successfully developed through glucosaminic acid-surface modification of iron oxide nanoparticles. The resultant glucosaminic acid-modified magnetic nanoparticles (GA-MNPs) had not only good uniformity in spherical shape with diameter of about 10-13 nm, but also possessed excellent water-dispersity and stability. In cell culture experiments, the internalization of GA-MNPs into different kinds of cells was observed over a 5-day period. The results indicated that the internalization of GA-MNPs into mouse macrophage cells and mouse embryonic fibroblast cells was not observed after 40 h of culturing. However, the GA-MNPs were internalized quickly into cancer cells after just 24 h of culturing. TEM images of the GA-MNPs uptake in ECA-109 cells were used to study the internalization mechanisms of GA-MNPs and their distribution in ECA-109 cells. Additionally, a water-dispersed magnetic capture probe was prepared by immobilization of oligonucleotides onto GA-MNPs, and the probe was used for detection and separation of their complementary oligonucleotides sequence.
Yu
Changjiang
C
Peking Union Medical College, Beijing 100730, People's Republic of China.
Zhao
Jianjun
J
Guo
Yingzhi
Y
Lu
Cailing
C
Ma
Xu
X
Gu
Zhongwei
Z
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D001710:Biotin / Q000737:chemistry; D002460:Cell Line; D005944:Glucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D006801:Humans; D008280:Magnetics; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009829:Oleic Acids / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D010316:Particle Size; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D009829:Oleic Acids; D009841:Oligonucleotides; D014867:Water; C035547:glucosaminic acid; D001710:Biotin; D005944:Glucosamine