title: Antioxidant and antigenotoxic activities of Korean fermented soybean.
abstract: This study was aimed at evaluating the antioxidative and antigenotoxic activities of Korean fermented soybean (Chungkookjang) in vitro and in vivo. The 100% ethanol extract of Chungkookjang (CKJ) inhibited the generation of 1,1-diphenyl-2-picryl hydrazine (DPPH) radicals, and had an inhibitory effect on LDL oxidation. CKJ and its constituents (genistein and daidzein) also inhibited H(2)O(2)-induced DNA damage from NIH/3T3 fibroblasts. Furthermore, they showed the cytoprotective effects against H(2)O(2)-induced cell death. In vivo study also demonstrated that an oral administration of CKJ extract (800 mg/kg/day) for 2 weeks potently inhibited the formation of malondialdehyde, the damage of DNA and the formation of micronucleated reticulocytes in KBrO(3)-treated mice. The well-known antioxidants, trolox and vitamin C, also showed the potent inhibition on these parameters. All these results indicate that CKJ extract may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage. The isoflavones, genistein and daidzein, may contribute to these biological effects of CKJ extract at least in part. Korean fermented soybean (Chungkookjang) is suggested to be a promising functional food witch can prevent oxidative stress.
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon, Republic of Korea.
authors.forename: Nam Yee
authors.initials: NY
authors.lastname: Kim
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Song
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Hyun Pyo
authors.initials: HP
authors.lastname: Kim
authors.forename: Moon Young
authors.initials: MY
authors.lastname: Heo
mesh_terms: D016475:3T3 Cells; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D005285:Fermentation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D013025:Glycine max
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D010936:Plant Extracts