[Application of accelerated solvent extraction technique for analysis of active components in traditional Chinese medicinal herbs].
The application of accelerated solvent extraction (ASE) technique for the Analysis of active components in traditional Chinese medicinal herbs was introduced by using two kinds of herbs as examples. The conditions including extraction temperature, static extraction time, the ratio of material to solvent and solvent of ASE for extraction of salvianolic acid B in Salvia miltiorrhiza were optimized by orthogonal experiments, and the optimal conditions were obtained. Different extraction methods (ASE, steam distillation, ultrasonic wave and Soxhlet extraction) were used to extract volatile oil in Aucklandia lappa Decne. Results of the comparative experiments indicated that ASE was the most effective method in this case. All the results from these studies demonstrate that ASE is indeed a powerful tool in the preparation of herbal extracts for downstream chromatographic analysis.
Chen
Junhui
J
Qingdao Key Laboratory on Analytical Technology Development and Standardization of Traditional Chinese Medicines, The First Institute of Oceanography of State Oceanic Administration, Qingdao 266061, China. chen2000junhui@163.com
Yang
Baijuan
B
Li
Wenlong
W
Wang
Xiaoru
X
Lee
Frank Sen-Chun
FS
Yang
Huanghao
H
D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D053630:Depsides
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D012997:Solvents; C076944:salvianolic acid B; D053630:Depsides