PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation.
OBJECTIVE
To explore the expression of PlexinA1 in gastric carcinoma and its relationship with tumor angiogenesis and proliferation.


METHODS
PlexinA1 mRNA and protein expressions of Semaphorin6D were measured using semi-quantity reverse transcription PCR and Western blotting in 20 cases of gastric carcinoma and corresponding normal gastric mucosa. PlexinA1, Ki-67 expression and microvessel density (MVD) were detected by immunohistochemistry in 50 cases of gastric carcinoma and 20 cases of normal gastric mucosa.


RESULTS
The mRNA and protein expressions of PlexinA1 in gastric carcinoma were significantly higher than that in normal gastric mucosa (0.71 +/- 0.37 vs 0.60 +/- 0.25, P = 0.0299 < 0.05, and 0.47 +/- 0.16 vs 0.21 +/- 0.08, P = 0.0000 < 0.01), and MVD within tumor tissues increased significantly with PlexinA1 mRNA expression (r =0.8736, P < 0.01) and PlexinA1 protein expression (r = 0.7286, P < 0.01), and MVD of the PlexinA1 positive staining group (25.25 +/- 3.93) was significantly higher than that of the negative group (19.56 +/- 1.75), (P < 0.01). Proliferation index of tumor cells within tumor tissues were positively correlated with PlexinA1 mRNA expression (r = 0.5420, P = 0.014 < 0.01) and PlexinA1 protein expression (r = 0.5024, P = 0.024 < 0.05). The proliferation index of the PlexinA1 positive staining group (567.69 +/- 125.61) was significantly higher than that of the negative group (369.58 +/- 116.88), (P < 0.01).


CONCLUSIONS
PlexinA1 may play an important role in the occurrence and development of gastric carcinoma, and be related to tumor angiogenesis and proliferation.
Zhao
Xiang-Yang
XY
Department of General Surgery, General Hospital of Chinese PLA, 28 Fuxing Road, Beijing 100853, China.
Chen
Lin
L
Li
Yu-Hong
YH
Xu
Qian
Q
D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D009419:Nerve Tissue Proteins; C492116:PLXNA1 protein, human; D012333:RNA, Messenger; D011956:Receptors, Cell Surface