title: Isolation and chemiluminescent properties of ferret (Mustela putorius furo) polymorphonuclear cells.
abstract: Ferret polymorphonuclear cells (PMNs) and peripheral blood mononuclear cells (PBMCs) were separated from whole blood by density gradient centrifugation. Using a 50% Percoll solution (density=1.066), PMNs and PBMCs were successfully isolated after centrifugation; the purities of the PMNs and PBMCs were 94.2% and 95.6%, respectively. To evaluate the function of isolated ferret PMNs, we measured the superoxide generation with a MCLA-dependent chemiluminescence assay. The isolated ferret PMNs responded to phorbol 12-myristate 13-acetate (PMA) with kinetics similar to that of human PMNs. The ferret PMNs did not respond to N-formyl-Met-Leu-Phe (fMLF), unlike human PMNs, which rapidly responded. Thus, authors established a method for the rapid separation of highly purified populations of functional PMNs from the whole blood of ferrets.
authors.affiliation: Nihon University Veterinary Research Center, Kanagawa, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakata
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000818:Animals; D005289:Ferrets / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology; D049449:Luminescence; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*