title: Synthesis of pyrophosphates for in vitro selection of catalytic RNA molecules.
abstract: Reported here are synthetic routes to pyrophosphates linking riboflavin with various nucleosides. The focus is on a flavin-uracil dinucleotide having a biotin tag on the uracil, a molecule that has potential value in the selection of RNA enzymes that catalyze the template-directed polymerization of RNA in the 3'-to-5' direction, which is the direction opposite that catalyzed by standard protein polymerases. Two detailed procedures are presented to prepare this new compound, as well as one procedure to prepare the new flavin-2,6-diaminopurine dinucleotide.
authors.affiliation: Foundation for Applied Molecular Evolution, Gainesville, Florida, USA.
authors.forename: Hyo-joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Myong Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Nilesh
authors.initials: N
authors.lastname: Karalkar
authors.forename: Daniel
authors.initials: D
authors.lastname: Hutter
authors.forename: Steven A
authors.initials: SA
authors.lastname: Benner
mesh_terms: D001710:Biotin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D011756:Diphosphates / Q000138:chemical synthesis* / Q000737:chemistry; D005415:Flavins / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*; D012256:Riboflavin / Q000737:chemistry
chemical_list: D011756:Diphosphates; D005415:Flavins; D009705:Nucleosides; D016337:RNA, Catalytic; D001710:Biotin; D012256:Riboflavin