Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp.
We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.
Rhee
Hyun-Woo
HW
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
Lee
Chang-Ro
CR
Cho
Seung-Hyon
SH
Song
Mi-Ryung
MR
Cashel
Michael
M
Choy
Hyon E
HE
Seok
Young-Jae
YJ
Hong
Jong-In
JI
D001419:Bacteria / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006158:Guanosine Pentaphosphate / Q000032:analysis*; D011721:Pyrenes / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D014867:Water / Q000737:chemistry
C526602:(1-((pyren-1-yl)propoxy)-3,5-bis(bis(2-pyridylmethyl)aminomethyl)benzene)zinc(II); D005456:Fluorescent Dyes; D011721:Pyrenes; D011725:Pyridines; D014867:Water; D006158:Guanosine Pentaphosphate