Group-specific quantification of methanotrophs in landfill gas-purged laboratory biofilters by tyramide signal amplification-fluorescence in situ hybridization.
The aim of this study was to quantitatively analyse methanotrophs in two laboratory landfill biofilters at different biofilter depths and at temperatures which mimicked the boreal climatic conditions. Both biofilters were dominated by type I methanotrophs. The biofilter depth profiles showed that type I methanotrophs occurred in the upper layer, where relatively high O(2) and low CH(4) concentrations were present, whereas type II methanotrophs were mostly distributed in the zone with high CH(4) and low O(2) concentrations. The number of type I methanotrophic cells declined when the temperature was raised from 15 degrees C to 23 degrees C, but increased when lowered to 5 degrees C. A slight decrease in type II methanotrophs was also observed when the temperature was raised from 15 degrees C to 23 degrees C, whereas cell numbers remained constant when lowered to 5 degrees C. The results indicated that low temperature conditions favored both type I and type II methanotrophs in the biofilters.
Wang
Hong
H
Department of Biological and Environmental Science, P.O. Box 35, 40014 University of Jyväskylä, Finland. whong@cc.jyu.fi
Einola
Juha
J
Heinonen
Mirja
M
Kulomaa
Markku
M
Rintala
Jukka
J
D000577:Amides / Q000737:chemistry*; D001483:Base Sequence; D017931:DNA Primers; D005374:Filtration / Q000295:instrumentation*; D005740:Gases; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D017013:Methanobacteriaceae / Q000302:isolation & purification*; D015345:Oligonucleotide Probes; D012037:Refuse Disposal
D000577:Amides; D017931:DNA Primers; D005740:Gases; D015345:Oligonucleotide Probes