Rapid active assay for the detection of antibodies to West Nile virus in chickens.
To reduce the assay time for detecting virus-specific antibodies in serum, we developed microarray-based active immunoassay techniques for detecting West Nile virus (WNV)-specific IgM molecules in chicken blood. The assay uses electrophoretic concentration of IgM molecules onto WNV antigens arrayed on a dialysis membrane followed by detection of bound IgM molecules with functionalized magnetic beads as active labels. This assay takes only 15 minutes and has the same sensitivity as a commercially available human WNV IgM antibody-capture enzyme-linked immunosorbent assay (commonly called a MAC-ELISA) modified for use with chicken sera.
Groves
Stephanie S
SS
The National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, VA 20110, USA. sgroves1@gmu.edu
Turell
Michael J
MJ
Bailey
Charles L
CL
Morozov
Victor N
VN
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D011201:Poultry Diseases / Q000097:blood / Q000175:diagnosis* / Q000517:prevention & control; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012680:Sensitivity and Specificity; D014901:West Nile Fever / Q000175:diagnosis / Q000662:veterinary*; D014902:West Nile virus / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic