title: Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis.
abstract: The gene coding for a thermophilic neutral protease from Bacillus stearothermophilus was expressed in Bacillus subtilis DB104, under the control of the sacB gene promoter. This was followed by either the native signal peptide sequence of this protease or the signal peptide sequence of the sacB gene. The protease was purified 3.8-fold, with a specific activity of 16530 U mg(-1). As analyzed by SDS-PAGE, the molecular mass of the expressed protease was about 35 kDa, and the optimal temperature and pH of the protease were 65 degrees C and 7.5, respectively. Moreover, it still had about 80% activity after 1 h reaction at 65 degrees C.
authors.affiliation: College of Engineering, Shenyang Agricultural University, Shenyang 110161, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Cong
authors.initials: C
authors.lastname: Zhao
authors.forename: LianXiang
authors.initials: L
authors.lastname: Du
authors.forename: FuPing
authors.initials: F
authors.lastname: Lu
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D015870:Gene Expression / Q000235:genetics*; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D013696:Temperature
chemical_list: D006867:Hydrolases