title: Newly developed chemical probes and nano-devices for cellular analysis.
abstract: A cellular analyzing system including a "real-time cellular imaging system" and a "comprehensive analyzing system for cellular responses" was developed. A "real-time cellular imaging system" is a system used to measure real-time imaging of multiple phenomena of a single cell with high special and temporal resolutions for the purpose to understand the pathology and physiology in a single cell and realize to single cell level diagnosis. A "real-time cellular imaging system" includes multi-probe imaging with AFM (atomic force microscopy), optical and SECM (scanning electrochemical microscopy) modes, which provides us with topological information and biochemical reactions at the local area of the interior and exterior of a cell. Scanning electrochemical/optical microscopy was applied to image PC12 cells. On the other hand, cells respond to their specific substances via their ligands. Therefore, the comprehensive analysis of protein-protein interaction is the important issue to determine the functions of cells. For this purpose, a "comprehensive analysis system for cellular responses" was developed. This system is based on SPR (surface plasmon resonance) and MS (mass spectrometry) using a nano-fabricated substrate. The interaction between IL-1 beta and anti-IL-1 beta antibodies was detected.
authors.affiliation: Core Research for Evolutional Science and Technology of Japan Science and Technology, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Honda
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Komatsu
authors.forename: Dai
authors.initials: D
authors.lastname: Kato
authors.forename: Akio
authors.initials: A
authors.lastname: Ueda
authors.forename: Kenichi
authors.initials: K
authors.lastname: Maruyama
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ito
authors.forename: Osamu
authors.initials: O
authors.lastname: Niwa
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D002477:Cells / Q000648:ultrastructure*; D003199:Computer Systems; D004563:Electrochemistry; D005456:Fluorescent Dyes; D006801:Humans; D013058:Mass Spectrometry; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques; D036103:Nanotechnology / Q000639:trends*
chemical_list: D005456:Fluorescent Dyes