Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads.
In the present study, the optimum conditions for the production of xylanase by immobilized spores of Trichoderma reesei SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12+/-0.18 U ml(-1) at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36+/-0.2 U ml(-1)).
Kar
Sanjay
S
Department of Microbiology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Mandal
Asish
A
Mohapatra
Pradeep K Das
PK
Samanta
Saptadip
S
Pati
Bikash R
BR
Mondal
Keshab C
KC
D000464:Alginates / Q000737:chemistry*; D019149:Bioreactors; D018914:Cells, Immobilized / Q000201:enzymology; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry* / Q000378:metabolism*; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007194:India; D007218:Industrial Microbiology; D008863:Microspheres; D013172:Spores, Fungal / Q000201:enzymology; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology* / Q000302:isolation & purification
D000464:Alginates; D005656:Fungal Proteins; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D043364:Endo-1,4-beta Xylanases