title: Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering.
abstract: Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promote bone repair in vivo when combined with porous scaffolds based on poly(l-lactic acid) (PLA) obtained by supercritical gas foaming and reinforced with 5 wt.% beta-tricalcium phosphate (TCP). Bone regeneration was assessed by radiography and histology after implantation of PLA/TCP scaffolds alone, seeded with primary fetal bone cells, or coated with demineralized bone matrix. Craniotomy critical size defects and drill defects in the femoral condyle in rats were employed. In the cranial defects, polymer degradation and cortical bone regeneration were studied up to 12 months postoperatively. Complete bone ingrowth was observed after implantation of PLA/TCP constructs seeded with human fetal bone cells. Further tests were conducted in the trabecular neighborhood of femoral condyles, where scaffolds seeded with fetal bone cells also promoted bone repair. We present here a promising approach for bone tissue engineering using human primary fetal bone cells in combination with porous PLA/TCP structures. Fetal bone cells could be selected regarding osteogenic and immune-related properties, along with their rapid growth, ease of cell banking and associated safety.
authors.affiliation: Institute of Translational Biomechanics, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
authors.forename: Marc-Olivier
authors.initials: MO
authors.lastname: Montjovent
authors.forename: Silke
authors.initials: S
authors.lastname: Mark
authors.forename: Laurence
authors.initials: L
authors.lastname: Mathieu
authors.forename: Corinne
authors.initials: C
authors.lastname: Scaletta
authors.forename: Arnaud
authors.initials: A
authors.lastname: Scherberich
authors.forename: Claire
authors.initials: C
authors.lastname: Delabarde
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Zambelli
authors.forename: Pierre-Etienne
authors.initials: PE
authors.lastname: Bourban
authors.forename: Lee Ann
authors.initials: LA
authors.lastname: Applegate
authors.forename: Dominique P
authors.initials: DP
authors.lastname: Pioletti
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones / Q000166:cytology* / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000033:anatomy & histology*; D006801:Humans; D020007:Implants, Experimental; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C485817:beta-tricalcium phosphate; D019344:Lactic Acid; C033616:poly(lactide)