Acidovorax caeni sp. nov., a denitrifying species with genetically diverse isolates from activated sludge.
Four Gram-negative, rod-shaped, non-spore-forming, denitrifying isolates were obtained from the activated sludge of an aerobic-anaerobic wastewater treatment plant in Belgium. Analysis of repetitive sequence-based PCR showed that the four isolates were genetically different from each other. Results of 16S rRNA gene sequence analysis and DNA-DNA hybridization experiments indicated that these four isolates were affiliated to the genus Acidovorax and could be differentiated from all recognized species of the genus. Analysis of whole-cell proteins and results of physiological and biochemical tests allowed differentiation of the new isolates from their closest phylogenetic neighbours. These new isolates therefore represent a novel species of the genus Acidovorax, for which the name Acidovorax caeni sp. nov. is proposed. The type strain is R-24608(T) (=LMG 24103(T) =DSM 19327(T)).
Heylen
Kim
K
Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, Ghent University, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium. Kim.Heylen@UGent.be
Lebbe
Liesbeth
L
De Vos
Paul
P
D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001530:Belgium; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity; D014865:Waste Disposal, Fluid / Q000379:methods
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D012722:Sewage