[The effect of specific interleukin-1 receptor blockade on hematopoietic stem cell proliferation].
Interleukin-1 plays a key role in the regulation of hematopoiesis. One of the major controlling mechanisms of IL-1 activites is mediated through IL-1ra. In this report we investigated whether IL-1ra might function as a true negative growth regulator of hematopoietic cells. The effect of rIL-1ra on the proliferation of early- and late-appearing CFU-S was assessed in regenerating bone marrow during the recovery after sublethal damage by X-irradiation. To this, regenerating bone marrow cells of CBA/H mice, obtained 3 days after 2 Gy irradiation, were preincubated 90 min to 5 hr with increasing concentrations of rIL-1ra (5-100 ng/ml). To determine the proliferative activity of CFU-Sd12 and CFU-Sd8, cytosine-arabinoside was added for the last 1 hr of incubation. The results revealed significant dose-dependent inhibition of CFU-Sd12, but not of CFU-Sd8 cycling after IL-1 receptor blackade with rIL-1ra over 10 ng/ml. The data implicate IL-1 receptor involvement in the proliferative activity of hematipoietic stem cells, but suggest that prevention of cell cycling by IL-1ra depends on the position of target cells in the heterogenous stem cell compartment.
Stosić-Grujicić
S
S
Milenković
P
P
Ivanović
Z
Z
Lukić
M
M
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D003561:Cytarabine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D017472:Receptors, Interleukin-1 / Q000502:physiology
D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D003561:Cytarabine