Correlation between expression of DcR3 on tumor cells and sensitivity to FasL.
To investigate the correlation between sensitivity to Fas ligand (FasL) and expression level of decoy receptor 3 (DcR3) on tumor cell surface, Fas/DcR3 mRNA expression was detected by RT-PCR. Anti-DcR3 mAb was used to detect expression level of DcR3 on surface of tumor cells by flow cytometry. Caspase-8, caspase-9, caspase-3, Bcl-2 expressions were analyzed by Western blot, respectively. Sensitivity to apoptosis induced by FasL was determined by Annexin V apoptosis kit. The expressions of DcR3 on the surface of tumor cells from high to low were approximately 35.3% in BGC823 cells, and 21.6% in MCF-7 cells, respectively. The apoptotic rates induced by FasL from low to high were 15.6% in BGC823 cells, and 58.2% in MCF-7 cells, respectively. There was a significant correlation between the expression levels of DcR3 with FasL-inducing apoptosis.
Li
Wenzhu
W
Anti-Cancer Research Center, Medical College, Xiamen University, 422 Siming South Road, Xiamen, Fujian 361005, China.
Zhang
Changgong
C
Chen
Caixia
C
Zhuang
Guohong
G
D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D051017:Apoptosis Regulatory Proteins / Q000276:immunology* / Q000378:metabolism*; D045744:Cell Line, Tumor; D053222:Fas Ligand Protein / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D053323:Receptors, Tumor Necrosis Factor, Member 6b / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology
D051017:Apoptosis Regulatory Proteins; D053222:Fas Ligand Protein; D053323:Receptors, Tumor Necrosis Factor, Member 6b; D011994:Recombinant Proteins