N-acylhomoserine lactone regulates violacein production in Chromobacterium violaceum type strain ATCC 12472.
In tests, Chromobacterium violaceum ATCC 12472 produced several N-acyl-L-homoserine lactones (AHLs). Of these, N-(3-hydroxydecanoyl)-L-homoserine lactone was dominant, and controlled violacein production by quorum sensing. Strain VIR07, an AHL-deficient mutant, did not produce violacein. Violacein production in VIR07 was induced by adding long-chain AHLs (C10-C16), but was inhibited by adding short-chain AHLs (C4-C8). Strain VIR07 showed the response of violacein production when AHLs diffused from a variety of AHL-producing bacteria on agar plates, and thus might be a useful biosensor for recognizing exogenous AHLs.
Morohoshi
Tomohiro
T
Department of Applied Chemistry, Faculty of Engineering, Utsunomiya University, Yoto, Utsunomiya, Japan. morohosi@cc.utsunomiya-u.ac.jp
Kato
Masashi
M
Fukamachi
Katsumasa
K
Kato
Norihiro
N
Ikeda
Tsukasa
T
D054742:Acyl-Butyrolactones / Q000737:chemistry / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D002861:Chromobacterium / Q000235:genetics / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D009154:Mutation; D053038:Quorum Sensing / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D054742:Acyl-Butyrolactones; D007211:Indoles; C063155:violacein