A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression.
A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.
Obro
Nina Friesgaard
NF
Department of Veterinary Pathobiology, University of Copenhagen, Ridebanevej, Frederiksberg, Denmark.
Lademann
Ulrik
U
Birkenkamp-Demtroder
Karin
K
Holten-Andersen
Lars
L
Brünner
Nils
N
Offenberg
Hanne
H
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics* / Q000378:metabolism
D020033:Protein Isoforms; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1