title: Mutagenic, antimutagenic, cytotoxic, and apoptotic activities of extracts from Pituranthos tortuosus.
abstract: Mutagenic and antimutagenic activities against direct acting mutagens, nifuroxazide (NF) and sodium azide (SA), and indirect acting mutagen aflatoxin B1 (AFB1) of extracts prepared from aerial parts of Pituranthos tortuosus were investigated in bacterial assay systems (i.e., the Ames test with Salmonella typhimurium TA100, TA98, TA1538, TA1535, and the SOS chromotest with Escherichia coli PQ 37). It was found that all extracts obtained from P. tortuosus decreased the mutagenicity induced by AFB1 (10 microg/assay), SA (1.5 microg/assay), and NF (20 microg/assay). Ethyl acetate, acetone, methanol, and total oligomer flavenoid extracts exhibited the highest inhibition level of mutagenicity induced by the indirect mutagen AFB1. In addition, antiproliferative and apoptotic properties of these extracts have also been reported using two leukemia cell lines, L1210 and K562. The results revealed that all extracts showed a significant cytotoxic effect on these cell lines, and the effect was greater in the presence of human K562 chronic myelogenous leukemia cells, whereas they do not induce apoptosis.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire, Faculté de Pharmacie de Monastir, Monastir, Tunisie.
authors.forename: Afef
authors.initials: A
authors.lastname: Abdelwahed
authors.forename: Ines
authors.initials: I
authors.lastname: Skandrani
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: A
authors.initials: A
authors.lastname: Neffati
authors.forename: Mohamed Ben
authors.initials: MB
authors.lastname: Sghaier
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Rebaï Ben
authors.initials: RB
authors.lastname: Ammar
authors.forename: Amor
authors.initials: A
authors.lastname: Mahmoud
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D016604:Aflatoxin B1 / Q000494:pharmacology; D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D019661:Apiaceae / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004269:DNA, Bacterial / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D062385:Hydroxybenzoates / Q000494:pharmacology; D020014:K562 Cells; D051379:Mice; D009153:Mutagens / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D009154:Mutation / Q000187:drug effects; D009581:Nitrofurans / Q000494:pharmacology; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D019810:Sodium Azide / Q000494:pharmacology
chemical_list: D016587:Antimutagenic Agents; D000972:Antineoplastic Agents, Phytogenic; D004269:DNA, Bacterial; D062385:Hydroxybenzoates; D009153:Mutagens; D009581:Nitrofurans; D010936:Plant Extracts; D019810:Sodium Azide; D016604:Aflatoxin B1; C013150:nifuroxazide