Prostaglandin F(2alpha) regulates cytokine responses of mast cells through the receptors for prostaglandin E.
There is an increasing body of evidence that prostanoids modulate mast cell functions and contribute to the development of allergic inflammation. The present study aimed to identify an undetermined function of prostaglandin (PG) F(2alpha) in mast cell activation and the signaling mechanism involved in it. Simultaneous quantification of prostanoids by liquid chromatography/tandem mass spectrometry revealed the constitutive release of PGF(2alpha), thromboxane B(2), and 6-keto-PGF(1alpha) from bone marrow-derived mast cells (BMMCs). Upon activation of BMMCs by lipopolysaccharide, the cytokine production in BMMCs was enhanced when the culture was supplemented with PGF(2alpha). However, F prostanoid receptor-a selective receptor for PGF(2alpha)-was not detected in BMMCs. Further investigations performed using prostanoid receptor antagonists revealed an alternative mechanism wherein the receptors for PGE species-E prostanoid receptors-mediated the PGF(2alpha) signal in BMMCs. The present study provides an insight into a novel function of PGF(2alpha), i.e., an autocrine accelerator for mast cell activation.
Kaneko
Izumi
I
Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
Hishinuma
Takanori
T
Suzuki
Kaori
K
Owada
Yuji
Y
Kitanaka
Noriko
N
Kondo
Hisatake
H
Goto
Junichi
J
Furukawa
Hiroshi
H
Ono
Masao
M
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D019898:Autocrine Communication / Q000187:drug effects; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000187:drug effects / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013929:Thromboxane B2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
D017077:Culture Media, Conditioned; D016207:Cytokines; D008070:Lipopolysaccharides; D011453:Prostaglandins; D018078:Receptors, Prostaglandin E; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost