New values of molecular extinction coefficient and specific rotation for cyanobacterial toxin cylindrospermopsin.
The molecular extinction coefficient epsilon of cylindrospermopsin (CYN) purified by the anion exchange and the normal-phase HPLC procedures was determined to be 9800 at 262 nm. This epsilon is significantly higher than those (epsilon, 5800-6250) reported previously. In order to determine CYN concentrations in solutions using UV absorption, the epsilon-value of CYN should be corrected from 5800 to 9800. Further, the [alpha](D) value of CYN should be corrected from +12.5 degrees to +17.0 degrees .
Sano
Tomoharu
T
National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan.
Kikuchi
Sachiko
S
Kubo
Takuya
T
Takagi
Hiroo
H
Hosoya
Ken
K
Kaya
Kunimitsu
K
D000470:Alkaloids; D000818:Animals; D001427:Bacterial Toxins; D000458:Cyanobacteria / Q000378:metabolism*; D000087522:Cyanobacteria Toxins; D015394:Molecular Structure; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; C089595:cylindrospermopsin; D014498:Uracil