title: Endothelial cell loss after Descemet's stripping endothelial keratoplasty in a large prospective series.
abstract: OBJECTIVE
  To report the donor endothelial cell loss in the first year after Descemet's stripping endothelial keratoplasty (DSEK) for the treatment of endothelial dysfunction.
  
  
  METHODS
  Prospective noncomparative interventional case series.
  
  
  METHODS
  Eighty eyes of 78 patients with corneal edema.
  
  
  METHODS
  Eighty eyes with endothelial failure were entered into a prospective study of endothelial keratoplasty (EK). The donor central endothelial cell density (ECD) was recorded postoperatively at 6 months (n = 80) and 12 months (n = 80) and then compared with the preoperative eye bank measurements. The subsets of eyes with the donor prepared manually (DSEK; n = 19) and the donor prepared with a microkeratome (Descemet's stripping automated EK [DSAEK]; n = 61) were also evaluated and compared.
  
  
  METHODS
  Preoperative and postoperative central ECDs were prospectively evaluated and the cell loss calculated for each postoperative time point.
  
  
  RESULTS
  The average and standard deviation ECD at 6 months was 1908+/-354 cells/mm(2), representing a mean cell loss from preoperative donor cell measurements of 34+/-12%. At 12 months, ECD was 1856+/-371 cells/mm(2) (35+/-13% cell loss). The 1% additional cell loss from 6 to 12 months was not significant (P = 0.233). In the subset of DSEK eyes (n = 19), the cell loss from preoperatively to 6 months was 34%, and at 12 months it was 39%. In the subset of DSAEK eyes (n = 61), the cell loss from preoperatively to 6 months was 34%, and at 12 months it was 34%. There was no statistical difference between the cell loss from DSEK and that from DSAEK at 6 months (P = 0.884) or at 12 months (P = 0.224).
  
  
  CONCLUSIONS
  Descemet's stripping EK using our surgical technique has a mean donor endothelial cell loss of 34% at the 6-month postoperative examination, and this average cell loss remains relatively stable up to at least 1 year. We found no difference in cell loss between the DSEK and DSAEK techniques over this 1-year postoperative period.
authors.affiliation: Corneal Services, Devers Eye Institute, Portland, Oregon 97210, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Terry
authors.forename: Edwin S
authors.initials: ES
authors.lastname: Chen
authors.forename: Neda
authors.initials: N
authors.lastname: Shamie
authors.forename: Karen L
authors.initials: KL
authors.lastname: Hoar
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Friend
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D002470:Cell Survival; D015715:Corneal Edema / Q000601:surgery; D016039:Corneal Transplantation / Q000009:adverse effects*; D003886:Descemet Membrane / Q000601:surgery*; D004728:Endothelium, Corneal / Q000473:pathology* / Q000637:transplantation*; D005260:Female; D005642:Fuchs' Endothelial Dystrophy / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications; D011446:Prospective Studies; D014019:Tissue Donors