title: The measurement of serum ceruloplasmin is useful for diagnostic differentiation of immune thrombocytopenic purpura.
abstract: BACKGROUND
  To expand the criteria of immune thrombocytopenic purpura (ITP), which is a diagnosis of exclusion, we analyzed proteins separated by 1-dimensional gel electrophoresis of the retained fraction in a Con A-Sepharose column from ITP patients' sera.
  
  
  METHODS
  Serum samples were from 19 adult patients with chronic ITP, 9 patients with thrombocytopenia of decreased production, and 20 healthy controls. Samples were applied to a Con A-Sepharose column, and the retained fraction was subjected to 10% SDS-PAGE. The % area of each densitometric protein peak was compared between the two groups and proteins in each band were identified using LC-MS/MS.
  
  
  RESULTS
  Eleven protein bands were distinctly separated by 1-dimensional electrophoresis. The percent area of bands #2 and #3 were significantly higher in ITP patients than in controls. The percent area of band #2 (p<0.01) was significantly higher in ITP patients than in non-ITP patients. We identified alpha(2)-macroglobulin, ceruloplasmin (Cp), and C3 in band #2 and complement factor B in #3 band. Serum concentrations of alpha(2)-macroglobulin and Cp were significantly higher in ITP patients than in controls. Serum concentrations of Cp were significantly higher in ITP patients than in non-ITP patients (p=0.0005). Serum complement factor B concentrations were significantly higher in ITP patients and non-ITP patients than in controls. ROC analysis showed that the total percent area of bands #2 and #3, and Cp had higher diagnosis availability for ITP patients when compared with controls and non-ITP patients, respectively.
  
  
  CONCLUSIONS
  Measurement of Cp separated by the present method could be useful for the diagnosis of ITP in the presence of thrombocytopenia and a non- or low-inflammatory state.
authors.affiliation: Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, Gifu City, Japan.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Chousa
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ito
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Saito
authors.forename: Kanako
authors.initials: K
authors.lastname: Takahashi
authors.forename: Masao
authors.initials: M
authors.lastname: Takemura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tomita
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Seishima
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D002570:Ceruloplasmin / Q000032:analysis*; D003937:Diagnosis, Differential; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000175:diagnosis*; D012372:ROC Curve
chemical_list: D002570:Ceruloplasmin