[Induction of ecto-5'-nucleotidase of rat mesangial cells in culture].
It is very well known that macrophages via their secretory products act on many mesangial cell activities. IL-1b (interleukin-1b), tumor necrosis factor alfa (TNF-a) and prostoglandin E2 (PGE2) effect on rat mesangial cell ecto-enzymes: ecto-5'-nucleotidase and ecto-Mg++-ATP-ase were investigated. Ecto-5'-nucleotidase activity was increased in a dose dependent manner after treatment for 24 and 48 hours. Maximum increases reached 4.5 times, 1.7 times and 2.2 times basal values for IL-1b (20 IU/ml), TNF-a (25 ng/ml) and PGE2 (0.1-10 uM) respectively. Ecto-Mg++-ATP-ase activity was unchanged. Cycloheximide (a protein synthesis inhibitor) bloked these stimulatory effects until cyclo-oxigenase inhibitors (indomethacine, ibuprofen and aspirin) diminished activity aproximately 50%. These results indicate that induction of ecto-5'-nucleotidase activity is mainly dependent of protein synthesis and only particulary of increased PGE2 activity.
Savić V
Vlahović
P
P
Stefanović
V
V
D015720:5'-Nucleotidase / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D015232:Dinoprostone / Q000494:pharmacology; D004790:Enzyme Induction; D053583:Interleukin-1beta / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D050527:Mesangial Cells / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D017077:Culture Media, Conditioned; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D015720:5'-Nucleotidase; D015232:Dinoprostone