Molecular cloning and expression profiling of the first specific jasmonate biosynthetic pathway gene allene oxide synthase from Lonicera japonica.
In jasmonate biosynthetic pathway, allene oxide synthase (AOS, EC 4.2.1.92), which is a cytochrome P450 (CYP74A), catalyzes the first committed step. We herein cloned a novel cDNA from Lonicera japonica Thunb., named LjAOS (GenBank accession: DQ303120), which was homologous to other AOSs. Southern blot analysis revealed that it was a multi-copy gene. Real-time quantitative PCR analysis showed that LjAOS mRNA accumulated most abundantly in alabastrums, in which the content of chlorogenic acid (CA, the major important active ingredient indicator) was previously proven to be the highest.
Jiang
Keji
K
State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Pi
Yan
Y
Hou
Rong
R
Zeng
Hainian
H
Huang
Zhuoshi
Z
Zhang
Zheng
Z
Sun
Xiaofen
X
Tang
Kexuan
K
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D003517:Cyclopentanes / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D031239:Lonicera / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D054883:Oxylipins / Q000378:metabolism*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; C011006:jasmonic acid; D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase