[Studies on bioassay-guided anti-inflammatory fraction in bark of Albizia julibrissin combined determination with LC-MS-MS].
OBJECTIVE
To search the anti-inflammatory fraction of Albizia julibrissin.


METHODS
Inflammatory model of Kunming mice ear edema induced by croton oil and determination combined with the LC-MS-MS-guided fractionation and isolation were used.


RESULTS
The n-butanol fraction (AJ-B) obtained from the ethanolic extract of the Cortex albiziae was the major active fraction. The lignan glycosides fraction (AJ-B-1), which was further isolated from AJ-B, showed significant anti-inflammatory activity and exhibited dose-dependent relationship in the dose of 5 to 20 mg x kg(-1).


CONCLUSIONS
The method of bioassay-guided fractionation and isolation combined with the LC-MS-MS determination may be of benefit to the logical studies on the bioactive fractions or constituents of traditional Chinese materia medica.
Qiao
Shan-Yi
SY
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. crbj611202@sina.com
Yu
Dong-Hong
DH
Guo
Ji-Fen
JF
Zhao
Yi-Min
YM
D031289:Albizzia / Q000737:chemistry*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use; D001681:Biological Assay / Q000379:methods; D000440:Butanols; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003436:Croton Oil; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use; D017705:Lignans / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000440:Butanols; D004365:Drugs, Chinese Herbal; D006027:Glycosides; D017705:Lignans; D003436:Croton Oil