title: Matrix formalism for site-specific binding of unstructured proteins to multicomponent lipid membranes.
abstract: We describe a new approach to calculate the binding of flexible peptides and unfolded proteins to multicomponent lipid membranes. The method is based on the transfer matrix formalism of statistical mechanics recently described as a systematic tool to study DNA-protein-drug binding in gene regulation. Using the energies of interaction of the individual polymer segments with different membrane lipid species and the scaling corrections due to polymer looping, we calculate polymer adsorption characteristics and the degree of sequestration of specific membrane lipids. The method is applied to the effector domain of the MARCKS (myristoylated alanine rich C kinase substrate) protein known to be involved in signal transduction through membrane binding. The calculated binding constants of the MARCKS(151-175) peptide and a series of related peptides to mixed PC/PS/PIP2 membranes are in satisfactory agreement with in vitro experiments.
authors.affiliation: Institute of Bioorganic Chemistry, Belarus National Academy of Sciences, Kuprevich 5/2, Minsk 220141, Belarus. teif@fh.huji.ac.il
authors.forename: Vladimir B
authors.initials: VB
authors.lastname: Teif
authors.forename: Daniel
authors.initials: D
authors.lastname: Harries
authors.forename: Dmitri Y
authors.initials: DY
authors.lastname: Lando
authors.forename: Avinoam
authors.initials: A
authors.lastname: Ben-Shaul
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008566:Membranes; D008956:Models, Chemical; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D010455:Peptides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000502:physiology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008055:Lipids; D008565:Membrane Proteins; D010455:Peptides; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D011506:Proteins; D000076250:Myristoylated Alanine-Rich C Kinase Substrate