Experimental validation for quantitative protein network models.
Cellular responses are the consequence of complex reactions of protein networks. The complexity should ultimately be described by a set of formulas in a quantitative fashion, in which each formula defines the reactions in response to given types of input. However, testing these formulas has not been a simple task because of the lack of appropriate means for experimental validation. 'Reverse-phase' lysate microarrays have been proved to be powerful for such requirements and thus can be a good resource for providing an experimental reference point for the theoretical biology of protein networks.
Nishizuka
Satoshi
S
Molecular Translational Technology, Molecular Therapeutics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 1126B, 9000 Rockville Pike, Bethesda, MD 20892, USA. nishizus@mail.nih.gov
Spurrier
Brett
B
D001709:Biotechnology; D006801:Humans; D008954:Models, Biological; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
D011506:Proteins