title: Influence of systematically varied nano-scale topography on cell morphology and adhesion.
abstract: The types of cell-matrix adhesions and the signals they transduce strongly affect the cell-phenotype. We hypothesized that cells sense and respond to the three-dimensionality of their environment, which could be modulated by nano-structures on silicon surfaces. Human foreskin fibroblasts were cultured on nano-structures with different patterns (nano-post and nano-grate) and heights for 3 days. The presence of integrin alpha(5), beta(1), beta(3), paxillin and phosphorylated FAK (pFAK) were detected by western blot and immunofluorescence. Integrin beta(3) exhibited stronger signals on nano-grates. pFAK and paxillin were observed as small dot-like patterns on the cell-periphery on nano-posts and as elongated and aligned patterns on nano-grates. Collectively, our observations highlighted the presence of focal (integrin beta(1), beta(3), pFAK, paxillin), fibrillar (integrin alpha(5), beta(1)) and 3-D matrix (integrin alpha(5), beta(1), paxillin) adhesions on nano-structures. The presented nano-structures offer interesting opportunities to study the interaction of cells with topographical features comparable to the size of extracellular matrix components.
authors.affiliation: Department of Surgery, Regenerative Bioengineering and Repair Laboratory, University of California Los Angeles, Los Angeles, California 90095-1741, USA.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Heydarkhan-Hagvall
authors.forename: Chang-Hwan
authors.initials: CH
authors.lastname: Choi
authors.forename: James
authors.initials: J
authors.lastname: Dunn
authors.forename: Sanaz
authors.initials: S
authors.lastname: Heydarkhan
authors.forename: Katja
authors.initials: K
authors.lastname: Schenke-Layland
authors.forename: W Robb
authors.initials: WR
authors.lastname: MacLellan
authors.forename: Ramin E
authors.initials: RE
authors.lastname: Beygui
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D016023:Integrins / Q000378:metabolism; D049329:Nanostructures / Q000648:ultrastructure*; D051419:Paxillin / Q000378:metabolism; D010766:Phosphorylation; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D015815:Cell Adhesion Molecules; D016023:Integrins; D051419:Paxillin; D011956:Receptors, Cell Surface; D051416:Focal Adhesion Protein-Tyrosine Kinases