Detection of cancer-related proteins in fresh-frozen ovarian cancer samples using laser capture microdissection.
Tumors are heterogeneous structures that contain different cell populations. Laser capture microdissection (LCM) can be used to obtain pure cancer cells from fresh-frozen cancer tissue and the surrounded environment, thus providing an accurate snapshot of the tumor and its microenvironment in vivo. We describe a new approach to isolate pure cancer cell population and evaluate protein expression. The process includes immunocytochemistry, laser microdissection, and western blot analysis. Using this technique, we can detect proteins such as X-linked inhibitor of apoptosis protein (XIAP) and Fas ligand (FasL) with as little as 1000 cells.
Silasi
Dan-Arin
DA
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
Alvero
Ayesha B
AB
Mor
Jechiel
J
Chen
Rui
R
Fu
Han-Hsuan
HH
Montagna
Michele K
MK
Mor
Gil
G
D014408:Biomarkers, Tumor / Q000032:analysis; D015925:Cryopreservation; D016616:Cryoultramicrotomy / Q000379:methods; D053222:Fas Ligand Protein / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D053594:Myeloid Differentiation Factor 88 / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D010051:Ovarian Neoplasms / Q000473:pathology*; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000032:analysis
D014408:Biomarkers, Tumor; C505661:FASLG protein, human; D053222:Fas Ligand Protein; C507779:MYD88 protein, human; D053594:Myeloid Differentiation Factor 88; D009363:Neoplasm Proteins; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human