title: Effect of methacrylic monomers on phagocytes reactive oxygen species: a possible BDDMA modulating action.
abstract: In the last years the studies regarding the biocompatibility of dental materials investigate, in addition to the classic cytotoxic tests, the interactions between the materials and the host cells to better explain the causes of the adverse effects observed sometimes in the clinical practice. In the present study the ability of diurethane dimethacrylate (DUDMA) and 1,4-butanediol dimethacrylate (BDDMA) methacrylic monomers present in dental composite resins to alter the functionality of peripheral blood monocytes (PBMs) and polymorphonucleate cells (PMNs) was examined. These cells are involve in the biological response to materials and in the host ability to respond to bacteria. The results obtained suggest that the examined methacrylates induce a relevant decrease of PBMs oxidative burst whereas the basal ROS production is only slightly decreased. In PMNs DUDMA induces a decrease of both basal and stimulated ROS production. BDDMA, on the contrary, it does not alter total oxidative burst in presence of stimulus while induces a statistically significant decrease of basal ROS production. Moreover this monomer alters the reaction kinetics of stimulated ROS production. The reported finding seems to indicate that this molecule could be able to stabilize PMNs in resting state and maximize their stimulated activity.
authors.affiliation: Università Cattolica del Sacro Cuore, Istituto di Biochimica e Biochimica Clinica, Rome, Italy. g.nocca@rm.unicatt.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Nocca
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lupi
authors.forename: Fabio
authors.initials: F
authors.lastname: De Santis
authors.forename: Bruno
authors.initials: B
authors.lastname: Giardina
authors.forename: Francesco
authors.initials: F
authors.lastname: De Palma
authors.forename: Claudio
authors.initials: C
authors.lastname: Chimenti
authors.forename: Gianluca
authors.initials: G
authors.lastname: Gambarini
authors.forename: Pasquale
authors.initials: P
authors.lastname: De Sole
mesh_terms: D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008163:Luminescent Measurements / Q000379:methods; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D015394:Molecular Structure; D010084:Oxidation-Reduction; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000187:drug effects*; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D014520:Urethane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity
chemical_list: D003764:Dental Materials; D008689:Methacrylates; D017382:Reactive Oxygen Species; C528099:diurethane dimethacrylate; D014520:Urethane; C102737:1,4-butanediol dimethacrylate