title: Titania sol-gel-derived tyrosinase-based amperometric biosensor for determination of phenolic compounds in water samples. Examination of interference effects.
abstract: For detection of phenolic compounds in environmental water samples we propose an amperometric biosensor based on tyrosinase immobilized in titania sol-gel. The analytical characteristics toward catechol, p-cresol, phenol, p-chlorophenol, and p-methylcatechol were determined. The linear range for catechol determination was 2.2 x 10(-7)-1.3 x 10(-5) mol L(-1) with a limit of detection of 9 x 10(-8) mol L(-1) and sensitivity 2.0 x 10(3) mA mol(-1) L. The influence of sample matrix components on the electrode response was studied according to Plackett-Burman experimental design. The potential interferents Mg(2+), Ca(2+), HCO3(-), SO4(2-), and Cl(-), which are usually encountered in waters, were taken into account in the examination. Cu(2+) was also taken into account, because CuSO(4) is sometimes added to a water sample, as a preservative, before determination of phenolic compounds. It was found that among the ions tested only Mg(2+) and Ca(2+) did not directly affect the electrode response. The developed biosensor was used for determination of catechol in spring and surface water samples using the standard addition method.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Kraków, Poland. kochana@chemia.uj.edu.pl
authors.forename: J
authors.initials: J
authors.lastname: Kochana
authors.forename: A
authors.initials: A
authors.lastname: Gala
authors.forename: A
authors.initials: A
authors.lastname: Parczewski
authors.forename: J
authors.initials: J
authors.lastname: Adamski
mesh_terms: D000363:Agaricales / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D044367:Phase Transition; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D013696:Temperature; D014025:Titanium / Q000737:chemistry*; D014867:Water
chemical_list: D004800:Enzymes, Immobilized; D010636:Phenols; D014867:Water; C009495:titanium dioxide; D014025:Titanium; D014442:Monophenol Monooxygenase