Application of a statistical design to the optimization of parameters and culture medium for alpha-amylase production by Aspergillus oryzae CBS 819.72 grown on gruel (wheat grinding by-product).
The production optimization of alpha-amylase (E.C.3.2.1.1) from Aspergillus oryzae CBS 819.72 fungus, using a by-product of wheat grinding (gruel) as sole carbon source, was performed with statistical methodology based on three experimental designs. The optimisation of temperature, agitation and inoculum size was attempted using a Box-Behnken design under the response surface methodology. The screening of nineteen nutrients for their influence on alpha-amylase production was achieved using a Plackett-Burman design. KH(2)PO(4), urea, glycerol, (NH(4))(2)SO(4), CoCl(2), casein hydrolysate, soybean meal hydrolysate, MgSO(4) were selected based on their positive influence on enzyme formation. The optimized nutrients concentration was obtained using a Taguchi experimental design and the analysis of the data predicts a theoretical increase in the alpha-amylase expression of 73.2% (from 40.1 to 151.1 U/ml). These conditions were validated experimentally and revealed an enhanced alpha-amylase yield of 72.7%.
Kammoun
Radhouane
R
Centre de Biotechnologie de Sfax, Laboratoire d'Enzymes et Métabolites des Procaryotes, Route de sidi Mansour km 4, BP"K" 3038 Sfax, Tunisia. radhouan.kammoun@cbs.rnrt.tn
Naili
Belgacem
B
Bejar
Samir
S
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis; D013172:Spores, Fungal / Q000201:enzymology; D014908:Triticum; D018505:Waste Management / Q000379:methods*; D000516:alpha-Amylases / Q000096:biosynthesis*
D003470:Culture Media; D005656:Fungal Proteins; D000516:alpha-Amylases