title: Sensitivity and specificity of recombinant omega-5 gliadin-specific IgE measurement for the diagnosis of wheat-dependent exercise-induced anaphylaxis.
abstract: BACKGROUND
  A recent study has shown that the measurement of specific IgE antibodies to B-cell epitope peptides of wheat omega-5 gliadin (Pep A) and high molecular weight glutenin subunit (Pep B) are useful to diagnose wheat-dependent exercise-induced anaphylaxis (WDEIA).
  
  
  OBJECTIVE
  We sought to compare the sensitivity and specificity of the in vitro tests for measuring the specific IgE antibodies to recombinant omega-5 gliadin (romega-5 gliadin) with those for wheat, gluten, Pep A, and Pep B in identification of patients with WDEIA.
  
  
  METHODS
  Fifty patients with WDEIA, 25 healthy subjects and 25 patients with atopic dermatitis with specific IgE antibodies to wheat but without experience of allergic reactions after ingestion of wheat products were enrolled in this study. The concentrations of specific IgE antibodies were measured using ImmunoCAP. The empirical receiver operating characteristics curves (ROC) for each test were prepared and the areas under the ROC curve (AUC) were compared.
  
  
  RESULTS
  In patients with WDEIA, the sensitivities of the allergen-specific IgE tests for wheat, gluten, Pep A, Pep B and romega-5 gliadin were 48%, 56%, 76%, 22%, and 80%, respectively. The seven of 10 WDEIA patients with no specific IgE antibodies to romega-5 gliadin had specific IgE antibodies to Pep B. The highest AUC (0.850) was observed in the test for romega-5 gliadin.
  
  
  CONCLUSIONS
  Measuring the concentration of specific IgE antibodies to romega-5 gliadin is more useful than to wheat, gluten, or Pep A in the identification of patients with WDEIA.
authors.affiliation: Division of Clinical Pharmacotherapeutics, Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsuo
authors.forename: J
authors.initials: J
authors.lastname: Dahlström
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Kohno
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Furumura
authors.forename: E
authors.initials: E
authors.lastname: Morita
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000235:genetics / Q000276:immunology*; D000707:Anaphylaxis / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D000918:Antibody Specificity; D052179:Antigens, Plant; D019540:Area Under Curve; D002648:Child; D015444:Exercise; D005260:Female; D005903:Gliadin / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D012680:Sensitivity and Specificity; D021182:Wheat Hypersensitivity / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D011994:Recombinant Proteins; C475180:omega-5 gliadin, wheat; D007073:Immunoglobulin E; D005903:Gliadin