[Effect of aldosterone on aquaporin and ionophorous protein expressions in guinea pig cochlea].
OBJECTIVE
To study the effect of aldosterone on aquaporin and ionophorous protein expressions in the cochlea of guinea pigs.


METHODS
RT-PCR was used to detect the expression of Na-K ATPase and epithelial sodium channel (ENaC) mRNA in the cochlea of guinea pigs 6 h after intraperitoneal aldosterone injection. Immunohistochemistry was used to detect the expression of aquaporin 1(AQP1) in the cochlea 1 month after the injection.


RESULTS
Early after aldosterone injection, the expression level of Na-K ATPase beta(1) and beta(3) subunit mRNAs remained unchanged and level of ENaC alpha subunit mRNA was up-regulated in the cochlear lateral wall (P<0.05), and 1 month after the injection, the expression of AQP1 protein was down-regulated (P<0.05).


CONCLUSIONS
The effect of aldosterone on the cochlea is mediated possibly through its action on the genome, and aldosterone may cause ion concentration alterations to induce endolymphatic hydrops.
Li
Qi
Q
Department of Otolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hxllq@126.com
Huang
De-Liang
DL
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism*; D053503:Epithelial Sodium Channels / Q000378:metabolism; D006168:Guinea Pigs; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D015854:Up-Regulation
D053503:Epithelial Sodium Channels; D012333:RNA, Messenger; D051398:Aquaporin 1; D000450:Aldosterone; D000254:Sodium-Potassium-Exchanging ATPase