title: Real-time polymerase chain reaction detection of Bordetella pertussis DNA in acellular pertussis vaccines.
abstract: Using 2 real-time polymerase chain reaction (PCR) assays for Bordetella pertussis, 2 of 5 acellular pertussis vaccines were found to contain B. pertussis DNA. Because residual DNA in vaccines can cause environmental contamination, the administration of acellular pertussis vaccines to patients should be physically separated from the collection of patients' specimens for testing of B. pertussis DNA by real-time PCR.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Division of Bacterial Diseases, Meningitis and Vaccine Preventable Diseases Branch, Atlanta, GA 30333, USA. ket2@cdc.gov
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Tatti
authors.forename: Barbara
authors.initials: B
authors.lastname: Slade
authors.forename: Manisha
authors.initials: M
authors.lastname: Patel
authors.forename: Nancy
authors.initials: N
authors.lastname: Messonnier
authors.forename: Tiffany
authors.initials: T
authors.lastname: Jackson
authors.forename: Kathryn B
authors.initials: KB
authors.lastname: Kirkland
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Talbot
authors.forename: M Lucia
authors.initials: ML
authors.lastname: Tondella
mesh_terms: D001886:Bordetella pertussis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D010567:Pertussis Vaccine / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D022282:Vaccines, Acellular / Q000737:chemistry*; D014917:Whooping Cough / Q000175:diagnosis
chemical_list: D004269:DNA, Bacterial; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular