title: Apoferritin as a bionanomaterial to facilitate the electron transfer reactivity of hemoglobin and the catalytic activity towards hydrogen peroxide.
abstract: In this report, apoferritin as a stable bionanomaterial was modified with hemoglobin on pyrolytic graphite electrode. Rapid electron transfer reactions of hemoglobin were achieved with the help of apoferritin in a large pH range. Moreover, hemoglobin as an enzyme exhibits fine electrocatalytic activity towards the reaction of hydrogen peroxide, and a wide concentration range of linear relationship between the reduction peak current and the concentration of hydrogen peroxide has been obtained with a higher upper detection limit, which may be further developed for a hydrogen peroxide biosensor. Therefore, a new property of apoferritin is explored, in which apoferritin works as a bionanomaterial to be an accelerant of the electron transfer of Hb and a stabilizer to retain the catalytic ability of the protein under mal-condition.
authors.affiliation: Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Chen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhu
authors.forename: Fanben
authors.initials: F
authors.lastname: Meng
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Yu
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D001052:Apoferritins / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002384:Catalysis; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; C009139:pyrolytic carbon; D002244:Carbon; D001052:Apoferritins; D006861:Hydrogen Peroxide