Formation and stability of a vinyl carbanion at the active site of orotidine 5'-monophosphate decarboxylase: pKa of the C-6 proton of enzyme-bound UMP.
Amyes
Tina L
TL
Department of Chemistry, University at Buffalo, Buffalo, New York 14260, USA.
Wood
Bryant M
BM
Chan
Kui
K
Gerlt
John A
JA
Richard
John P
JP
D001665:Binding Sites; D002244:Carbon / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000378:metabolism*; D011485:Protein Binding; D011522:Protons; D014542:Uridine Monophosphate / Q000737:chemistry* / Q000378:metabolism*; D014753:Vinyl Compounds / Q000737:chemistry*
D011522:Protons; D014753:Vinyl Compounds; D002244:Carbon; D014542:Uridine Monophosphate; D009964:Orotidine-5'-Phosphate Decarboxylase