The presence of blaIMP genes on plasmids DNA isolated from multidrug-resistant Pseudomonas aeruginosa strains at University Hospital in Bialystok (Poland)--first report.
Resistance to carbapenems is emerging, and it is a great problem to therapeutics. Seven multidrug-resistant (MDR) of Pseudomonas aeruginosa strains were isolated from urine and bronchial specimens. All isolates showed resistance to imipenem and meropenem (MIC; > or =16 mg/L). The resistance to carbapenems in two of seven strains was associated with the production of a metallo-beta-lactamases. Plasmids DNA probes were used to investigate the presence of genes coding for IMP-type enzymes. PCR experiments revealed that bla(IMP) genes were present in two isolates of Pseudomonas aeruginosa (MIC >32 microg/mL for both carbapenems).
Sacha
Paweł
P
Department of Microbiological Diagnostics, Medical University of Bialystok, Poland. sachpt@amb.edu.pl
Zórawski
Marcin
M
Hauschild
Tomasz
T
Wieczorek
Piotr
P
Jaworowska
Jadwiga
J
Jakoniuk
Piotr
P
Tryniszewska
Elzbieta
E
D015780:Carbapenems / Q000494:pharmacology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D006785:Hospitals, University; D006801:Humans; D010957:Plasmids / Q000302:isolation & purification*; D011044:Poland; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*
D015780:Carbapenems; D004269:DNA, Bacterial