A simple method for the production of anti-C3d monoclonal antibody.
Production of monoclonal antibodies to C3d usually involves the purification of protein. Our method does not require C3 purification; it relies on attachment of C3b to mouse erythrocytes by activation of alternative pathways and further conversion in C3d. We prepared human complement-coated mouse red cells and sensitized mice of the same strain with our own schedule of immunization and applied the classical methods to obtain a mouse monoclonal antibody. We obtained a clone called BMS-11 which produces a monoclonal antibody of IgM class, to C3d with a title of 1:500000. The monoclonal antibody obtained has shown that it is suitable for use as an antiglobulin reagent.
Cruz
Carlos
C
Banco M de Sangre del DC, Immunohematología, Caracas, Venezuela.
León
Graciela
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001202:Ascitic Fluid / Q000276:immunology; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology; D003179:Complement C3b / Q000378:metabolism; D015933:Complement C3d / Q000096:biosynthesis / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D003179:Complement C3b; D015933:Complement C3d