title: The exp1 gene essential for pileus expansion and autolysis of the inky cap mushroom Coprinopsis cinerea (Coprinus cinereus) encodes an HMG protein.
abstract: The homobasidiomycete Coprinopsis cinerea is a member of the fungi known as inky cap mushrooms, and its fruiting-body pileus autolyzes soon after completion of the development. During the last 3h of the development, the pileus exhibits umbrella-like expansion: the pileal tissue is cracked at the base of each gill and then each gill tissue is split to form a V-shape, as seen in a cross section. We identified two C. cinerea mutants defective in both pileus expansion and autolysis. The defects in both mutants are due to recessive mutations in a single gene, designated exp1. The exp1 gene is predicted to encode an HMG1/2-like protein with two HMG domains. The transcription of exp1 is strongly induced in the pileus 3h before pileus expansion. This result, together with the fact that the exp1 mutations cause a specific developmental phenotype, suggest that Exp1 is a novel, transcriptional regulator controlling the final phase of fruiting-body morphogenesis.
authors.affiliation: Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita 010-1195, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Muraguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Fujita
authors.forename: Yuya
authors.initials: Y
authors.lastname: Kishibe
authors.forename: Kanako
authors.initials: K
authors.lastname: Konno
authors.forename: Nanae
authors.initials: N
authors.lastname: Ueda
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nakahori
authors.forename: Sonoe O
authors.initials: SO
authors.lastname: Yanagi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamada
mesh_terms: D016386:Chromosome Walking; D003001:Cloning, Molecular; D003302:Coprinus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D010641:Phenotype; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D006609:High Mobility Group Proteins