Cathepsin L and cystatin B gene expression discriminates immune coelomic cells in the leech Theromyzon tessulatum.
Previous studies evidenced that cystatin B-like gene is specifically expressed and induced in large circulating coelomic cells following bacterial challenge in the leech Theromyzon tessulatum. In order to understand the role of that cysteine proteinase inhibitor during immune response, we investigated the existence of members of cathepsin family. We cloned a cathepsin L-like gene and studied its tissue distribution. Immunohistochemical studies using anti-cathepsin L and anti-cystatin B antibodies and ultrastructural results demonstrated the presence of three distinct coelomic cell populations: (1) the chloragocytes, which were initially defined as large coelomocytes, (2) the granular amoebocytes and (3) small coelomic cells. Among those cells, while chloragocytes contain cystatin B and cathepsin L, granular amoebocytes contain only cathepsin L and the third cell population contains neither cathepsin nor inhibitor. Finally, results evidenced that cathepsin L immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria.
Lefebvre
Christophe
C
Université de Lille 1, Laboratoire de Neuroimmunologie des Annélides, FRE 2933 Centre National de Recherche Scientifique (CNRS), IFR147, Bâtiment SN3, Université de Lille1, 59655 Villeneuve d'Ascq cedex, France. christophe.lefebvre@univ-lille1.fr
Vandenbulcke
Franck
F
Bocquet
Béatrice
B
Tasiemski
Aurélie
A
Desmons
Annie
A
Verstraete
Mathilde
M
Salzet
Michel
M
Cocquerelle
Claude
C
D000595:Amino Acid Sequence; D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D017124:Conserved Sequence; D055313:Cystatin B; D015891:Cystatins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004926:Escherichia coli; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007865:Leeches / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D016982:Micrococcus luteus; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010586:Phagocytes; D016415:Sequence Alignment
C524994:CSTB protein, human; D015891:Cystatins; D055313:Cystatin B; D002403:Cathepsins; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L