title: [Improvement of urinary d-glucaric acid assay and its application].
abstract: We propose an improved method to measure urinary D-glucaric acid (GA), which might be of value as an indirect index of the activity of cytochrome P450 (CYP). This method was about 20 times more sensitive than existing methods. Beer's law was obeyed in the concentration range 5.5-66 ng ml(-1) for GA, with the effective molar absorptivity at 533 nm and the relative standard deviation being 9.1 x 10(5) dm(3) mol(-1) cm(-1) and 0.69% (n = 6), respectively. In addition, we introduced the correction value {GA/Cr ratio x10} of urinary GA by measuring urinary creatinine (Cr) at the same time. Based on the proposed method, the GA and Cr values in spot urines of healthy persons and cancer patients were subsequently measured and the correction values of both groups subjected to comparison. As a result, a statistically significant difference was recognized between the two groups.
authors.affiliation: Department of Clinical Chemistry, Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki City, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Kumiyo
authors.initials: K
authors.lastname: Shimidzu
authors.forename: Shigehumi
authors.initials: S
authors.lastname: Morimoto
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Hachino
authors.forename: Kenji
authors.initials: K
authors.lastname: Hasegawa
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Shono
authors.forename: Tetuya
authors.initials: T
authors.lastname: Horiuchi
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Tabuse
authors.forename: Yurina
authors.initials: Y
authors.lastname: Masui
authors.forename: Takako
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Fujita
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000493:pharmacokinetics; D015415:Biomarkers / Q000652:urine; D003404:Creatinine / Q000652:urine; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004334:Drug Administration Schedule; D016903:Drug Monitoring; D005937:Glucaric Acid / Q000652:urine*; D006801:Humans; D008099:Liver / Q000201:enzymology; D009369:Neoplasms / Q000188:drug therapy / Q000652:urine
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D003577:Cytochrome P-450 Enzyme System; D003404:Creatinine; D005937:Glucaric Acid