Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of Sedum sarmentosum extract.
This study aimed to assess some novel pharmacological activities of Sedum sarmentosum Bunge, a perennial herb widely distributed on the mountain slopes in Oriental countries and traditionally used for the treatment of certain inflammatory diseases. Sedum sarmentosum was extracted with absolute methanol to generate the methanol extract (SS). SS exhibited a significant inhibitory activity in the chick embryo chorioallantoic membrane (CAM) angiogenesis in a dose-dependent manner (IC(50)=2.29 microg/egg). The anti-nociceptive activity of SS was demonstrated using acetic acid-induced writhing model in mice. SS reduced the levels of anti-inflammatory markers, such as volume of exudates, number of polymorphonuclear leukocytes and nitrite content, in the air pouch model. It dose-dependently exhibited an inhibitory activity in the acetic acid-induced vascular permeability in mice. It suppressed production of nitric oxide in the lipopolysaccharide (LPS)-activated RAW264.7 macrophages. Additionally, it suppressed induction of inducible nitric oxide synthase (iNOS) in the activated macrophages. In brief, the results provide some pharmacological basis for the therapeutic use of Sedum sarmentosum.
Jung
Hyun-Joo
HJ
College of Pharmacy, Sookmyung Women's University, Seoul 140-742, Republic of Korea.
Kang
Hyun-Jung
HJ
Song
Yun Seon
YS
Park
Eun-Hee
EH
Kim
Young-Myeong
YM
Lim
Chang-Jin
CJ
D000700:Analgesics / Q000494:pharmacology*; D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D031963:Sedum
D000700:Analgesics; D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D052247:Nitric Oxide Synthase Type II