title: Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp.
abstract: We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Rhee
authors.forename: Chang-Ro
authors.initials: CR
authors.lastname: Lee
authors.forename: Seung-Hyon
authors.initials: SH
authors.lastname: Cho
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Song
authors.forename: Michael
authors.initials: M
authors.lastname: Cashel
authors.forename: Hyon E
authors.initials: HE
authors.lastname: Choy
authors.forename: Young-Jae
authors.initials: YJ
authors.lastname: Seok
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Hong
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006158:Guanosine Pentaphosphate / Q000032:analysis*; D011721:Pyrenes / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: C526602:(1-((pyren-1-yl)propoxy)-3,5-bis(bis(2-pyridylmethyl)aminomethyl)benzene)zinc(II); D005456:Fluorescent Dyes; D011721:Pyrenes; D011725:Pyridines; D014867:Water; D006158:Guanosine Pentaphosphate