title: A conformational change in the alpha-subunit of coatomer induced by ligand binding to gamma-COP revealed by single-pair FRET.
abstract: Formation of transport vesicles involves polymerization of cytoplasmic coat proteins (COP). In COPI vesicle biogenesis, the heptameric complex coatomer is recruited to donor membranes by the interaction of multiple coatomer subunits with the budding machinery. Specific binding to the trunk domain of gamma-COP by the Golgi membrane protein p23 induces a conformational change that causes polymerization of the complex. Using single-pair fluorescence resonance energy transfer, we find that this conformational change takes place in individual coatomer complexes, independent of each other, and that the conformational rearrangement induced in gamma-COP is transmitted within the complex to its alpha-subunit. We suggest that capture of membrane protein machinery triggers cage formation in the COPI system.
authors.affiliation: Heidelberg University Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
authors.forename: Julian D
authors.initials: JD
authors.lastname: Langer
authors.forename: Christian M
authors.initials: CM
authors.lastname: Roth
authors.forename: Julien
authors.initials: J
authors.lastname: Béthune
authors.forename: Emily H
authors.initials: EH
authors.lastname: Stoops
authors.forename: Britta
authors.initials: B
authors.lastname: Brügger
authors.forename: Dirk-Peter
authors.initials: DP
authors.lastname: Herten
authors.forename: Felix T
authors.initials: FT
authors.lastname: Wieland
mesh_terms: D000818:Animals; D020756:Coatomer Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
chemical_list: D020756:Coatomer Protein; D005456:Fluorescent Dyes; D008024:Ligands; D046912:Multiprotein Complexes; D021122:Protein Subunits