[Biochemical and biophysical investigation of liposome action in artificially induced ARDS in rabbit lungs].
The aim of this study is to evaluate the application of phospholipid liposomes in HCl--induced RDS in rabbits. Acute respiratory distress syndrome was induced by administration of 0.2 N HCl via intratracheal instillation for 45 min. After induced ARDS animals under artificial lung ventilation were retreated with liposomes for 60 min. Arterial blood gas analysis was performed at 30, 45 and 60 min after liposome application. Untreated animals were ventilated for the same time. Rabbits were killed with thiopental and bronhoalveolar lavage fluid biochemical and biophysical parameters were investigated. HCl- lung injury caused decrease of arterial oxygen pressure/ fraction of inspired oxygen ratio more than 50% compared to the control. We obtained high respiratory acidosis as well. The instillation of liposomes led to reversion of gas exchange at 60 min. after application almost to the control value. In order to characterize the rabbit lung tissue changes after HCl-treatment histological and ultra thin slices were obtained. Electron microscopic preparations demonstrate disappearance of surface active film in treated animals. Application of liposomes led to visualization of osmophilic material forming lamellae in lamellar bodies. On the basis of the results obtained we may assume that it is likely that the liposomes assessed in this study might be used for in vivo improvement of oxygenation in acid aspiration induced ARDS.
Petkova
D
D
Steneva
I
I
Iordanova
A
A
Khristova
E
E
Lalchev
Z
Z
D000142:Acidosis, Respiratory / Q000097:blood / Q000209:etiology*; D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001784:Blood Gas Analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004195:Disease Models, Animal; D008081:Liposomes; D008168:Lung / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D010743:Phospholipids / Q000008:administration & dosage / Q000627:therapeutic use*; D011817:Rabbits; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000150:complications / Q000188:drug therapy* / Q000473:pathology
D008081:Liposomes; D010743:Phospholipids