DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.
In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed.
Zaparty
Melanie
M
Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany. melanie.zaparty@uni-due.de
Zaigler
Alexander
A
Stamme
Claudia
C
Soppa
Jörg
J
Hensel
Reinhard
R
Siebers
Bettina
B
D050260:Carbohydrate Metabolism; D019848:Gene Expression Regulation, Archaeal / Q000187:drug effects; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006019:Glycolysis; D006358:Hot Temperature; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics; D041542:Thermoproteus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D005947:Glucose