Oxidative stress is an important component of airway inflammation in mice exposed to cigarette smoke or lipopolysaccharide.
1. It was proposed previously that oxidative stress is a main component of the inflammatory process in chronic obstructive pulmonary disease (COPD). Thus, in the present study, we investigated the inflammatory response in mice deficient for the p47(phox) subunit of NADPH oxidase (p47 KO) exposed to cigarette smoke (CS). 2. Exposure of mice to CS elicited an increase in the number of macrophages and neutrophils and levels of interleukin (IL)-6, keratinocyte-derived chemokine (KC/CXCL1) and monocyte chemoattractant protein-1 (MCP1/CCL2) in bronchoalveolar lavage fluid (BALF), which were lower in p47 KO mice compared with control mice. In contrast, 24 h after lipopolysaccharide (LPS) exposure, the number of macrophages and neutrophils, as well as KC/CXCL1 levels, in BALF was significantly greater in p47 KO mice compared with control mice. 3. The present study has shown that airway inflammation is decreased in p47 KO mice after exposure to CS, but not LPS, suggesting that oxidative stress is involved in the pathogenesis of airway inflammation associated with COPD.
Lagente
Vincent
V
INSERM U620, Faculté de Pharmacie, Université de Rennes 1, Rennes, France. vincent.lagente@univ-rennes1.fr
Planquois
Jean-Michel
JM
Leclerc
Olivier
O
Schmidlin
Fabien
F
Bertrand
Claude P
CP
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced*; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
D016207:Cytokines; D008070:Lipopolysaccharides; D012906:Smoke; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1