title: Prevalence of estrogen receptor alpha PvuII and XbaI polymorphism in population of Polish postmenopausal women.
abstract: Numerous data indicate that polymorphism of estrogen receptor alpha (ERalpha) may predict lipid levels, lipid response to hormone replacement therapy (HRT), myocardial infarction risk, bone fracture risk, bone mineral density (BMD) and changes in BMD over time. In this study we aimed to evaluate distribution of ERalpha PvuII and XbaI genotypes in population of Polish postmenopausal women qualified to different protocols of HRT. Subject of the study were 64 consecutive postmenopausal women aged from 45 to 65 years (mean 56.6) assigned to HRT. ERalpha PvuII and XbaI polymorphism was determined by PCR-restriction fragment length polymorphism (RFLP). The absence of PvuII and XbaI restriction sites were indicated by "P" and "X" and presence by "p" and "x", respectively. PvuII genotype was distributed as follows: PP 17.2% (n=11), Pp 50% (n=32), pp 32.83% (n=21). Frequency of XbaI genotype was: XX 6.25% (n=4), Xx 34.4% (n=22), xx 59.4% (n=38). Four haplotypes with following frequencies were recognized: PX 17.3%, px 47.4%, Px 24.4% and pX 10.9%. Prevalence of estrogen receptor alpha PvuII and XbaI polymorphisms in Polish women is similar to previously studied population.
authors.affiliation: Department of Obstetrics and Gynecology, Central Clinical Hospital of Ministry of Interior and Administration, Warsaw, Poland. jakimiuk@yahoo.com
authors.forename: Artur
authors.initials: A
authors.lastname: Jakimiuk
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Nowicka
authors.forename: Michal
authors.initials: M
authors.lastname: Bogusiewicz
authors.forename: Aneta
authors.initials: A
authors.lastname: Adamiak
authors.forename: Pawel
authors.initials: P
authors.lastname: Skorupski
authors.forename: Pawel
authors.initials: P
authors.lastname: Miotla
authors.forename: Tomasz
authors.initials: T
authors.lastname: Rechberger
authors.forename: Jozef
authors.initials: J
authors.lastname: Haczynski
mesh_terms: D000368:Aged; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005006:Ethnicity; D005260:Female; D006239:Haplotypes; D006801:Humans; D008875:Middle Aged; D011044:Poland; D011110:Polymorphism, Genetic; D017698:Postmenopause / Q000235:genetics*; D044465:White People / Q000235:genetics*
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; C025580:endodeoxyribonuclease XBAI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific