title: Expression of alpha-galactosidase gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum.
abstract: A 2.5 kb aga gene encoding alpha-galactosidase (alpha- Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against alpha-Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the heterologous aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).
authors.affiliation: Institute of Agriculture and Life Science, Gyeongsang National University,Jinju 660-701, Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Seon-Ju
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Ae Ran
authors.initials: AR
authors.lastname: Lee
authors.forename: Ji Yeong
authors.initials: JY
authors.lastname: Park
authors.forename: Woo Ju
authors.initials: WJ
authors.lastname: Jeong
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D015153:Blotting, Western; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D018274:Electroporation; D015870:Gene Expression; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology; D000519:alpha-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D000519:alpha-Galactosidase