title: Proteomic analysis of the pH response in the fungal pathogen Candida glabrata.
abstract: Micro-organisms must adapt to environmental change to survive, and this is particularly true for fungal pathogens such as Candida glabrata. C. glabrata is found both in the environment and in diverse niches in its human host. The ambient pH of these niches varies considerably, and therefore we have examined the response of C. glabrata to changes in ambient pH using a proteomic approach. Proteins expressed in C. glabrata cells growing at pH 4.0, 7.4 or 8.0 were compared by 2-DE, and 174 spots displaying reproducible and statistically significant changes in expression level were identified by peptide mass fingerprinting, thereby extending our 2-DE map of the C. glabrata proteome to a total of 272 identified spots. Proteins involved in glucose metabolism, the TCA cycle, respiration and protein synthesis were expressed at lower levels during growth at pH 7.4 and/or 8.0, whereas proteins involved in stress responses and protein catabolism were expressed at higher levels under these alkaline conditions. Our data suggest that C. glabrata perceives low pH as less stressful than higher pH. This contrasts with another opportunistic fungal pathogen of humans, Candida albicans.
authors.affiliation: Institute of Medical Microbiology and National Reference Center for Systemic Mycoses, University of Göttingen, Göttingen, Germany.
authors.forename: Pia
authors.initials: P
authors.lastname: Schmidt
authors.forename: Janet
authors.initials: J
authors.lastname: Walker
authors.forename: Laura
authors.initials: L
authors.lastname: Selway
authors.forename: David
authors.initials: D
authors.lastname: Stead
authors.forename: Zhikang
authors.initials: Z
authors.lastname: Yin
authors.forename: Brice
authors.initials: B
authors.lastname: Enjalbert
authors.forename: Michael
authors.initials: M
authors.lastname: Weig
authors.forename: Alistair J P
authors.initials: AJ
authors.lastname: Brown
mesh_terms: D041221:Candida glabrata / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D020543:Proteome / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D020543:Proteome