Inhibitory effect of Ginkgo biloba extract on hyperhomocysteinemia-induced intimal thickening in rabbit abdominal aorta after balloon injury.
Ginkgo biloba extract (GBE) has been widely used to treat cardiovascular and cerebrovascular disorders. Hyperhomocysteinemia (Hhcy) is associated with the risk of atherosclerosis and restenosis after angioplasty. The objective of this study was to investigate whether GBE could attenuate the Hhcy-induced intimal thickening after balloon injury in rabbit abdominal aorta. It was observed in this study that GBE could decrease the neointima area (NA) and the ratio of the neointima area to the media area (NA/MA), down-regulate the mRNA expression of matrix metalloproteinase-9 (MMP-9) and up-regulate the protein expression of p21 (WAF1/CIP1) (p21). It suggests that GBE can reverse the Hhcy-induced neointima formation in rabbits following balloon injury, and the suppressive effect of GBE on the migration and proliferation of vascular smooth muscle cells (VSMCs) may contribute to its actions.
Liu
Fenye
F
Department of Traditional Chinese Medicine, Qilu Hospital Affiliated to Shandong University, Jinan 250012, Shandong, PR China.
Zhang
Jidong
J
Yu
Shudong
S
Wang
Rong
R
Wang
Bo
B
Lai
Liping
L
Yin
Huiqiu
H
Liu
Guilin
G
D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects* / Q000293:injuries; D015153:Blotting, Western; D002404:Catheterization / Q000009:adverse effects; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D020441:Ginkgo biloba / Q000737:chemistry*; D006710:Homocysteine / Q000097:blood; D020138:Hyperhomocysteinemia / Q000150:complications*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017539:Tunica Intima / Q000187:drug effects* / Q000473:pathology; D014652:Vascular Diseases / Q000097:blood / Q000209:etiology / Q000517:prevention & control
D050759:Cyclin-Dependent Kinase Inhibitor p21; D010936:Plant Extracts; D012333:RNA, Messenger; D006710:Homocysteine; D020780:Matrix Metalloproteinase 9