Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay.
Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a (3)H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.
Tanaka
Makito
M
Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
Takahashi
Yoshiyuki
Y
Xu
Yinyan
Y
Yoshida
Nao
N
Yoshimi
Ayami
A
Villalobos
Itzel Bustos
IB
Hama
Asahito
A
Nishio
Nobuhiro
N
Hidaka
Hirokazu
H
Wang
Yue
Y
Yagasaki
Hiroshi
H
Kojima
Seiji
S
D049109:Cell Proliferation; D002675:Child, Preschool; D005260:Female; D011905:Genes, ras; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D054429:Leukemia, Myelomonocytic, Juvenile / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000235:genetics; D013936:Thymidine / Q000378:metabolism*
D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D013936:Thymidine