title: Activity of adenosine deaminase and adenylate deaminase on adenosine and 2', 3(')-isopropylidene adenosine: role of the protecting group at different pH values.
abstract: The deamination rate of 2',3'-isopropylidene adenosine catalyzed by adenosine deaminase (ADA) from calf intestine and adenylate deaminase (AMPDA) from Aspergillus species has been evaluated and compared with that of the enzymatic reactions of adenosine, to elucidate the influence of the protecting group on enzyme activity.
authors.affiliation: Dipartimento di Scienze Precliniche LITA Vialba, Università degli Studi di Milano, Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Alessandrini
authors.forename: Pierangela
authors.initials: P
authors.lastname: Ciuffreda
authors.forename: Radmila
authors.initials: R
authors.lastname: Pavlovic
authors.forename: Enzo
authors.initials: E
authors.lastname: Santaniello
mesh_terms: D000659:AMP Deaminase / Q000737:chemistry / Q000378:metabolism*; D000241:Adenosine / Q000737:chemistry*; D000243:Adenosine Deaminase / Q000737:chemistry / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology; D002384:Catalysis; D003641:Deamination; D006863:Hydrogen-Ion Concentration
chemical_list: D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000241:Adenosine