Potential role of increased matrix metalloproteinase-2 (MMP2) transcription in impaired adipogenesis in type 2 diabetes mellitus.
We measured gene expression of paracrine regulators involved in adipocyte differentiation within the stromovascular fraction of abdominal subcutaneous adipose tissue from obese individuals with (n=30) and without (n=18) type 2 diabetes mellitus (T2DM). Despite similar adiposity by design, subjects with T2DM had larger adipocytes (0.92+/-0.28 vs. 0.75+/-0.17 microl, p<0.05) than controls. Gene expression of the adipogenic marker aP2 was lower (0.35+/-0.16 vs. 0.58+/-0.27 arbitrary units, p<0.05) whereas the expression of matricellular peptidase, MMP2 was higher (1.65+/-0.17 vs. 1.27+/-0.21, p=0.02) in T2DM vs. controls. The gene expression levels between the aP2 and MMP2 were inversely correlated (r=-0.32, p=0.03). We conclude that early steps of adipogenesis may be impaired in T2DM independently of obesity due, in part, to an upregulation of the MMP2 transcription.
Dubois
Severine G
SG
Health & Performance Enhancement Division, Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA 70808, USA.
Tchoukalova
Yourka D
YD
Heilbronn
Leonie K
LK
Albu
Jeanine B
JB
Kelley
David E
DE
Smith
Steven R
SR
Fang
Xiaobing
X
Ravussin
Eric
E
D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology*; D050156:Adipogenesis; D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000201:enzymology* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009765:Obesity / Q000150:complications / Q000201:enzymology* / Q000473:pathology*
C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2