[Differential proteomic analysis of nasopharyngeal carcinoma cell lines 5-8F and 6-10B].
OBJECTIVE
To compare the proteome difference of nasopharyngeal carcinoma (NPC) cell lines 5-8F and 6-10B, and to screen these proteins associated with NPC metastasis.


METHODS
Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background, respectively. PDQuest software was applied to analyze 2-DE images, and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.


RESULTS
2-DE maps of total proteins from 5-8F and 6-10B were established. A total of 65 differential protein spots in the 2 cell lines were found, and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B, which was consistent with the Results from the comparative proteomic analysis.


CONCLUSIONS
Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.
Xiang
Ya-li
YL
Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha 410008, China.
Yi
Hong
H
Li
Cui
C
Zhang
Peng-fei
PF
Li
Mao-yu
MY
Chen
Zhu-chu
ZC
Xiao
Zhi-qiang
ZQ
D002277:Carcinoma; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
D020543:Proteome