Cost-effective method of DNA extraction from taeniid eggs.
A new cost-effective method using silicon dioxide- and guanidine isothiocyanate-containing buffers, after previous alkaline lysis, was established for the DNA extraction from taeniid eggs isolated from canine faeces. The purified DNA can be used to amplify the species-specific 12S mitochondrial DNA of Echinococcus multilocularis in direct and nested polymerase chain reaction in order to differentiate between E. multilocularis and Taenia spp.
Dyachenko
V
V
Institute of Parasitology, University of Leipzig, An den Tierkliniken 35, 04103 Leipzig, Germany. dyachenko@vetmed.uni-leipzig.de
Beck
E
E
Pantchev
N
N
Bauer
C
C
D000818:Animals; D018509:DNA, Helminth / Q000032:analysis / Q000302:isolation & purification*; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D004443:Echinococcosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary; D048210:Echinococcus multilocularis / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D008969:Molecular Sequence Data; D010063:Ovum / Q000469:parasitology*; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods; D013045:Species Specificity; D013621:Taenia / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013622:Taeniasis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary
D018509:DNA, Helminth