Microbial conversion of ginsenoside Rb1 to minor ginsenoside F2 and gypenoside XVII by Intrasporangium sp. GS603 isolated from soil.
A new strain, GS603, having beta-glucosidase activity was isolated from soil of a ginseng field, and its ability to convert major ginsenoside Rb(1) to minor ginsenoside or gypenoside was studied. Strain GS603 was identified as an Intrasporangium species by phylogenetic analysis and showed high ginsenoside-converting activity in LB and TSA broth but not in nutrient broth. The culture broth of the strain GS603 could convert ginsenoside Rb(1 )into two metabolites, which were analyzed by TLC and HPLC and shown to be the minor ginsenoside F(2) and gypenoside XVII by NMR.
Cheng
Le-Qin
LQ
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Yongin 449-701, Korea.
Na
Ju-Ryun
JR
Kim
Myung Kyum
MK
Bang
Myun-Ho
MH
Yang
Deok-Chun
DC
D039903:Actinobacteria / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D036145:Ginsenosides / Q000737:chemistry / Q000378:metabolism*; D029774:Gynostemma / Q000737:chemistry / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D010802:Phylogeny; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012988:Soil Microbiology
D036145:Ginsenosides; D010936:Plant Extracts; D012336:RNA, Ribosomal, 16S; C407883:ginsenoside F2; C474918:gypenoside; C442759:ginsenoside Rb1