The influence of the hemodialysis treatment time under oxidative stress biomarkers in chronic renal failure patients.
Oxidative stress possibly helps to promote the progression and complication of chronic renal failure (CRF). Hemodialysis (HD) may aggravate oxidative stress. In addition long time of treatment may intensify the oxidative stress. Thus, the aim of this study was to evaluate the effect of prolonged HD treatment under parameters of the oxidative stress.


METHODS
Plasmatic thiobarbituric acid reactive substances (TBARS), plasmatic malondialdehyde (MDA), blood delta-aminolevulinate dehydratase (ALA-D) activity, ALA-D reactivation index, and erythrocytic reduced glutathione (GSH) were measured into two different groups of HD patients: recent treatment (n=36; HD duration: 17.7+/-1.71 months), and long time of treatment (n=26; HD duration: 82.2+/-6.32 months), and in a control group (n=40).


RESULTS
Plasmatic TBARS and MDA levels were both elevated in HD patients. However, only MDA levels had positive correlation with time of HD treatment. Blood ALA-D activity was decreased in HD patients. The ALA-D reactivation index showed increase in HD patients, and it had correlation with the time of HD treatment. Erythrocytic GSH levels were increased in HD patients.


CONCLUSIONS
Our results indicated that MDA levels and ALA-D reactivation index may be the better biomarkers to evaluate chronic oxidative stress in comparison with others markers analyzed in this study.
Valentini
Juliana
J
Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Campus Universitário, Caixa Postal 5061, CEP 97110-970, Santa Maria, Rio Grande do Sul, Brazil.
Grotto
Denise
D
Paniz
Clóvis
C
Roehrs
Miguel
M
Burg
Geni
G
Garcia
Solange C
SC
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000503:physiopathology* / Q000628:therapy; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D000623:Porphobilinogen Synthase / Q000097:blood; D006435:Renal Dialysis / Q000009:adverse effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013997:Time Factors
D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D000623:Porphobilinogen Synthase; D005978:Glutathione