title: Gradient HPLC of antibiotics in urine, ground water, chicken muscle, hospital wastewater, and pharmaceutical samples using C-18 and RP-amide columns.
abstract: A simple and highly sensitive high pressure liquid chromatographic (HPLC-UV) method has been developed for the determination of ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid, in mobile phase citrate buffer (0.001 M) of pH 4.5 prepared in water (X), methanol (Y), and ACN (Z) using gradient at a flow rate of 1.0 mL/min by direct UV absorbance detection at lambda = 280 nm. Separation of analytes was studied on the C-18 and RP-amide columns and best results were observed on the RP-amide column with LODs (3.3 x S/m) 0.89, 0.55, 0.67, and 1.41 ng/mL for ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid, respectively, and better RSD than the C-18 column. The recovery of Fluoroquinolones (FQs) in urine, ground water, hospital wastewater, and chicken muscle using this method is more than 90%. The method was successfully applied to the analysis of ofloxacin, lomefloxacin, cinoxacin, and nalidixic acid in urine, ground water, pharmaceutical dosage forms, hospital wastewater, and chicken muscle.
authors.affiliation: Department of Chemistry, Punjabi University, Patiala, India.
authors.forename: Ashwini
authors.initials: A
authors.lastname: Kumar
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar Malik
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Kumar Tewary
authors.forename: Baldev
authors.initials: B
authors.lastname: Singh
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000652:urine; D002138:Calibration; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007220:Industrial Waste; D009132:Muscles / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000652:urine
chemical_list: D000900:Anti-Bacterial Agents; D007220:Industrial Waste; D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical