An approach to assay calcineurin activity and the inhibitory effect of zinc ion.
In this study, an electrochemical method to assay calcineurin activity is proposed. Although the enzyme could not exhibit electron transfer reactivity and the catalytic reaction of the substrate could not give any electrochemical wave, p-nitrophenol as the catalytic reaction production could be oxidized at the calcineurin/Triton X-100 film modified electrode to exhibit useful wave that might be employed to assay the enzyme activity. The effect of Ni(2+) and Zn(2+) on calcineurin was also investigated. Whereas Ni(2+) was confirmed to be able to enhance the enzymatic activity, Zn(2+) was found to be an inhibitor to calcineurin.
Huang
Junyi
J
School of Life Science and Shanghai Key Laboratory of Bio-Energy Crops, Shanghai University, Shanghai 200444, People's Republic of China.
Zhang
Dongmei
D
Xing
Wei
W
Ma
Xiang
X
Yin
Yanxia
Y
Wei
Qun
Q
Li
Genxi
G
D019703:Calcineurin / Q000378:metabolism*; D065095:Calcineurin Inhibitors; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry; D004566:Electrodes; D006108:Graphite / Q000737:chemistry; D009532:Nickel / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D015032:Zinc / Q000494:pharmacology*
D065095:Calcineurin Inhibitors; D006108:Graphite; D009532:Nickel; D019703:Calcineurin; D015032:Zinc