title: Lack of association between the G-2548A polymorphism of the leptin gene and psoriasis in a Turkish population.
abstract: BACKGROUND
  Psoriasis is a multifactorial disease in which genetic and inflammatory factors play important roles. Leptin is classified as a cytokine and plays an important role in the regulation of the T-helper response. A common polymorphism in the promoter of the human leptin gene (G-2548A) may have a role in the pathogenesis of psoriasis.
  
  
  OBJECTIVE
  To investigate the association between psoriasis and leptin gene polymorphism (G-2548A).
  
  
  METHODS
  The study involved 109 patients with psoriasis and 125 healthy controls. Analyses of G-2548A polymorphism of the leptin gene were made by the polymerase chain reaction-restriction fragment length polymorphism technique. The genotypes (GG, GA, and AA of leptin gene G-2548A) and alleles (G and A) were scored and the frequencies were estimated. The frequencies of alleles and genotypes in patients and controls were compared. The relationship between leptin gene polymorphism and the clinical features of the patients was analyzed.
  
  
  RESULTS
  Both genotype [odds ratio (OR), 0.921; 95% confidence interval (CI), 0.501-1.694; P = 0.792] and allele (OR, 0.864; 95% CI, 0.600-1.242; P = 0.429) frequencies were not significantly different between patient and control groups. In addition, there was no significant association between genotype and allele frequencies and the clinical characteristics of psoriasis.
  
  
  CONCLUSIONS
  In this case-control study, no evidence of association between the G-2548A variant of the leptin gene and psoriasis was found.
authors.affiliation: Department of Medical Biology and Genetics, Ondokuz Mayis University, Samsun, Turkey. nurtenk@omu.edu.tr
authors.forename: Nurten
authors.initials: N
authors.lastname: Kara
authors.forename: Fatma
authors.initials: F
authors.lastname: Aydin
authors.forename: Nilgün
authors.initials: N
authors.lastname: Senturk
authors.forename: Sezgin
authors.initials: S
authors.lastname: Gunes
authors.forename: M Tayyar
authors.initials: MT
authors.lastname: Canturk
authors.forename: Hasan
authors.initials: H
authors.lastname: Bagci
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Bek
authors.forename: Ahmet Yasar
authors.initials: AY
authors.lastname: Turanli
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D020738:Leptin / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011565:Psoriasis / Q000235:genetics*; D014421:Turkey
chemical_list: D020738:Leptin