Pulsed electric field reduces the permeability of potato cell wall.
The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.
Galindo
Federico Gómez
FG
IBB-Institute for Biotechnology and Bioengineering, Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, Braga, Portugal. federico.gomez@deb.uminho.pt
Vernier
P Thomas
PT
Dejmek
Petr
P
Vicente
António
A
Gundersen
Martin A
MA
D002463:Cell Membrane Permeability / Q000502:physiology* / Q000528:radiation effects*; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D011829:Radiation Dosage; D011198:Solanum tuberosum / Q000502:physiology* / Q000528:radiation effects*