title: [Effect of Krüppel-like factor 4 overexpression on heat stress-induced apoptosis of Raw264.7 macrophages].
abstract: OBJECTIVE
  To observe the effect of Krüppel-like factor 4 (KLF4) overexpression on heat stress-induced apoptosis of Raw264.7 macrophages.
  
  
  METHODS
  The fragment containing full length mouse KLF4 cDNA coding sequence was inserted into the pcDNA3.1 vector and Raw264.7 macrophages were transfected with pcDNA3.1-KLF4 plasmids using lipofectamine.The expression of KLF4 was examined by Western blot in the Raw264.7 macrophages stably transfected with pcDNA3.1- KLF4 plasmids. Raw264.7 cells transfected with pcDNA3.1 and pcDNA3.1-KLF4 were exposed to heat stress (42 degrees C) for 1h and recovered at 37 degrees C for 12h. Flow cytometry, Hoechest 33258 staining assay, and DNA ladder assays were performed to assess the apoptosis.
  
  
  RESULTS
  The KLF4 overexpressed Raw264.7 macrophages were established. After the heat stress, flow cytometry showed that apoptotic cells increased significantly in KLF4 overexpressed cells compared with the vector control; Hoechest 33258 staining was characterized with classical changes including apoptotic body, and nuclear condensation. Agarose gel electrophoresis showed that "DNA ladder" could be observed clearly.
  
  
  CONCLUSIONS
  KLF4 overexpression can increase heat stress-induced apoptosis of Raw264.7 macrophages.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
authors.forename: Mei-dong
authors.initials: MD
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Jun-wen
authors.initials: JW
authors.lastname: Liu
authors.forename: Hua-li
authors.initials: HL
authors.lastname: Zhang
authors.forename: Xian-zhong
authors.initials: XZ
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line; D005822:Genetic Vectors; D018869:Heat-Shock Response; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D010957:Plasmids; D014162:Transfection
chemical_list: C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors