title: Development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine babesioses caused by Babesia bovis and Babesia bigemina.
abstract: With the objective of developing a simpler diagnostic alternative, a rapid immunochromatographic test (BoiICT) was constructed for the simultaneous detection of Babesia bovis- and Babesia bigemina-specific antibodies using B. bovis recombinant merozoite surface antigen-2c and B. bigemina recombinant rhoptry-associated protein-1. The BoiICT selectively detected specific antibodies to B. bovis and B. bigemina. All sera from cattle infected with other protozoan parasites (i.e., Cryptosporidium parvum, Neospora caninum, and Theileria orientalis) showed negative results in the BoiICT. The relative sensitivity and specificity for detecting antibody to B. bovis were 96.7% (29 of 30) and 91.3% (73 of 80), respectively. The relative sensitivity and specificity for detecting antibody to B. bigemina were 96.7% (29 of 30) and 92.5% (74 of 80), respectively. These findings indicate that the BoiICT is useful for fast field diagnostic assessment of bovine babesioses without any laboratory equipment.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan.
authors.forename: Chul-Min
authors.initials: CM
authors.lastname: Kim
authors.forename: Lidia Beatriz Conza
authors.initials: LB
authors.lastname: Blanco
authors.forename: Andy
authors.initials: A
authors.lastname: Alhassan
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iseki
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D001403:Babesia / Q000145:classification / Q000276:immunology*; D016793:Babesia bovis / Q000276:immunology; D001404:Babesiosis / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary
chemical_list: D000953:Antigens, Protozoan; D011933:Reagent Kits, Diagnostic