title: Rapid analysis of multiple pesticide residues in fruit-based baby food using programmed temperature vaporiser injection-low-pressure gas chromatography-high-resolution time-of-flight mass spectrometry.
abstract: A rapid method using programmed temperature vaporiser injection-low-pressure gas chromatography-high-resolution time-of-flight mass spectrometry (PTV-LP-GC-HR-TOF-MS) for the analysis of multiple pesticide residues in fruit-based baby food was developed. The fast and inexpensive buffered QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction method and "conventional" approach that employs ethyl acetate extraction followed by gel permeation chromatography (GPC) cleanup were employed for sample preparation. A PTV injector in solvent venting mode was used to reduce volume of acetonitrile and acetic acid (from the buffered QuEChERS extracts) that caused higher column bleed without their elimination. Otherwise, the time-to-digital converter would become saturated in HR-TOF-MS. For fast GC separation allowing analysis of 100 analytes within a 7 min runtime, both a high temperature programming rate and vacuum conditions in a megabore GC column were employed. The use of HR-TOF-MS allowed the unbiased identification and reliable quantification of target analytes through the application of a narrow mass window (0.02 Da) for extracting analyte ions and the availability of full spectral information even at very low levels. With only a few exceptions, the lowest calibration levels for the pesticides tested were <or=0.01 mg/kg for both sample preparation methods, which meets the EU maximum residue limit set for pesticide residues in cereal-based foods and baby foods (2003/13/EC).
authors.affiliation: Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, Department of Food Chemistry and Analysis, Technicka 3, 166 28 Prague 6, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Cajka
authors.forename: Jana
authors.initials: J
authors.lastname: Hajslova
authors.forename: Ondrej
authors.initials: O
authors.lastname: Lacina
authors.forename: Katerina
authors.initials: K
authors.lastname: Mastovska
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Lehotay
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000032:analysis; D002021:Buffers; D004726:Endosulfan / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006827:Hydantoins / Q000032:analysis; D007536:Isomerism; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D011312:Pressure; D015203:Reproducibility of Results; D013696:Temperature; D013997:Time Factors; D014835:Volatilization
chemical_list: D002021:Buffers; D006827:Hydantoins; D010573:Pesticide Residues; D000620:Aminoimidazole Carboxamide; D004726:Endosulfan; D019342:Acetic Acid; C033148:iprodione