title: [Identification and expression of haponin, a new protein from HL-60 cells].
abstract: We found a new protein haponin (an HLDF-alike protein) in promyelocyte HL-60 cells that is immunoreactive to polyclonal antibodies against HLDFbeta. Determination of the partial primary structure of the protein allowed us to reveal an immunogenic peptide of haponin and, on the basis of the amino acid sequence of this peptide, the degenerate primers were synthesized, which enabled us to clone the full-size cDNA of haponin. The stable heterologous expression of this cDNA in E. coli cells (Rosetta strain) was obtained. Preparations of natural and recombinant proteins exhibited antigenic cross-reactivity to polyclonal antibodies against this peptide.
authors.forename: A V
authors.initials: AV
authors.lastname: Vonarshenko
authors.forename: V V
authors.initials: VV
authors.lastname: Radchenko
authors.forename: M V
authors.initials: MV
authors.lastname: Gapon
authors.forename: I L
authors.initials: IL
authors.lastname: Rodionov
authors.forename: I I
authors.initials: II
authors.lastname: Babichenko
authors.forename: D L
authors.initials: DL
authors.lastname: Kakuev
authors.forename: I D
authors.initials: ID
authors.lastname: Artamonov
authors.forename: A V
authors.initials: AV
authors.lastname: Garkovenko
authors.forename: L G
authors.initials: LG
authors.lastname: D'iachkova
authors.forename: V M
authors.initials: VM
authors.lastname: Lipkin
authors.forename: I A
authors.initials: IA
authors.lastname: Kostanian
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D018922:HL-60 Cells; D006801:Humans; D024101:Mitochondrial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis
chemical_list: C526704:EIF1AD protein, human; D024101:Mitochondrial Proteins; D011506:Proteins; D011994:Recombinant Proteins