In vitro microTBS of one-bottle adhesive systems: sound versus artificially-created caries-affected dentin.
This in vitro study aimed to evaluate a pH-cycling model for simulation of caries-affected dentin (CAD) surfaces, by comparing the bond strength of etch-and-rinse adhesive systems on sound and artificially-created CAD. Dentin substrates with different mineral contents and morphological patterns were created by submitting buccal bovine dentin to the following treatments: (1) immersion in artificial saliva during the experimental period (sound dentin, SD), or (2) induction to a CAD condition by means of a dynamic pH-cycling model (8 cycles, demineralization for 3 h followed by mineralization for 45 h). The bond strength of Excite or Prime and Bond NT adhesive systems was assessed using the microtensile bond strength (microTBS) test. Dentin microhardness was determined by cross-sectional Knoop evaluations. Resin-dentin morphology after the treatments was examined by scanning electron microscopy. SD produced significantly higher microTBS than CAD for both adhesives evaluated, without differences between materials. CAD exhibited lower microhardness than SD. Morphological analysis showed marked distinctions between SD and CAD bonded interfaces. Under the conditions of this study, differences in morphological pattern and dentin mineral content may help to explain resin-dentin bond strengths. The proposed pH-cycling model may be a suitable method to simulate CAD surfaces for bonding evaluations.
Erhardt
Maria Carolina G
MC
Department of Restorative Dentistry, Piracicaba School of Dentistry, University of Campinas, São Paulo, Brazil.
Rodrigues
José Augusto
JA
Valentino
Thiago Assunção
TA
Ritter
André Vicente
AV
Pimenta
Luiz André Freire
LA
D000268:Adhesiveness; D000818:Animals; D002417:Cattle; D001840:Dental Bonding / Q000379:methods*; D003731:Dental Caries / Q000473:pathology*; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007180:Incisor / Q000473:pathology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D015233:Models, Statistical; D013499:Surface Properties; D013718:Tensile Strength
D017220:Dentin-Bonding Agents