Production of Poly(3-hydroxybutyrate) from waste potato starch.
There has been a considerable interest in using low cost carbon substrates for the production of Poly(3-hydroxybutyrate) (PHB). We have shown that saccharified waste potato starch can be used as a viable alternative carbon source in high cell density PHB production. Using Ralstonia eutropha NCIMB 11599 with phosphate limitation, 179 g/l biomass, 94 g/l PHB, Y(biomass/starch) = 0.46 g/g, Y(PHB/starch) = 0.22 g/g, and PHB productivity = 1.47 g/(l*h) were achieved. Residual maltose accumulated in the fed-batch reactor but caused no noticeable inhibition. Performance with saccharified starch was virtually identical to that with glucose.
Haas
Richard
R
Centre for Water Sciences and Systems, University of South Australia, Mawson Lakes, SA, Australia.
Jin
Bo
B
Zepf
Florian Tobias
FT
D020118:Cupriavidus necator / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006885:Hydroxybutyrates / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D010710:Phosphates / Q000378:metabolism; D011091:Polyesters / Q000302:isolation & purification* / Q000378:metabolism; D011198:Solanum tuberosum / Q000378:metabolism* / Q000382:microbiology*; D013213:Starch / Q000378:metabolism; D014866:Waste Products; D000094122:Polyhydroxybutyrates
D005947:Glucose; D006885:Hydroxybutyrates; D010710:Phosphates; D011091:Polyesters; D013213:Starch; D014866:Waste Products; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates