Bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine.
Adjuvants play an important role in the formulation of effective and appropriate vaccines. A series of synthetic bursin and bursin-like peptides was heterologously expressed in Escherichia coli. The use of bursin as an adjuvant enhanced the specific immune responses of mice immunized with a recombinant Japanese encephalitis virus E-binding domain (JEV E-BD) fusion protein. The effect was determined in the form of protective anti-JEV E titers, antibodies (IgG1 and IgG2a), spleen cell lymphocyte proliferation, the levels of interferon-gamma and interleukin-4 cytokines, and the T-lymphocyte sub-type composition. The IgG2a titer and interferon-gamma level suggested that the E-BD protein potentiates the Th1 immune response. These responses were changed when the immunogen was combined with one of the synthetic peptides as adjuvant. JEV-neutralization assay results show that the presence of bursin significantly enhance the JEV-neutralizing titer. We conclude that bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine, and represents a promising adjuvant for vaccination.
Wang
Chen
C
Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
Wen
Wen-Yan
WY
Su
Chun-Xia
CX
Ge
Fei-Fei
FF
Dang
Zhan-Guo
ZG
Duan
Xiang-Guo
XG
Cao
Rui-Bing
RB
Zhou
Bing
B
Chen
Pu-Yan
PY
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D009500:Neutralization Tests; D009842:Oligopeptides / Q000276:immunology*; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000166:cytology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D022321:Japanese Encephalitis Vaccines; D009842:Oligopeptides; D021122:Protein Subunits; D011994:Recombinant Proteins; C016281:bursopoietin