Effects of alpha-tocopherol on oxidative status and metabolic profile in overweight women.
Despite extensive research, the effects of alpha-tocopherol supplementation remain controversial. Few studies have been focused on obese and overweight people. We examined the effects of alpha-tocopherol (AT) on the oxidative status and metabolic profile in overweight women. Sixteen overweight women between the ages of 40-60 years old, received AT, 800 IU/day during 12 weeks, followed by a 6-week washout period. Blood samples were taken at the beginning and then every 6 weeks until the end of the study. AT, retinol, malondialdehyde (MDA), total antioxidant status (TAS), selenium-dependent glutathione peroxidase (GPx) and CuZn-superoxide dismutase (SOD) were quantified to evaluate the oxidative stress. The metabolic profile was estimated by measuring glycated hemoglobin (HbA1c) in erythrocytes and glucose, phosphate, magnesium, lipid and lipoprotein concentrations in serum. Under AT administration HbA1c, serum- MDA levels and erythrocyte GPx activity were markedly reduced. TAS, AT and Mg2+ concentrations in serum and SOD activity in erythrocytes were higher after AT treatment. Body weight; glucose, lipid and retinol concentrations, or blood cells count were unchanged. Lipid peroxidation was considerably reduced in AT treated women and also improved serum antioxidant status was observed, but the imbalanced response between erythrocyte SOD and GPx activities could affect normal response to oxidative stress.
Ble-Castillo
J L
JL
General Hospital, Zone 46, IMSS, Prolongación de Avenida Universidad Km 2.5, Colonia Casa Blanca, 86060, Villahermosa, Tabasco, México. jorgeble@hotmail.com
Cleva-Villanueva
G
G
Díaz-Zagoya
J C
JC
Medina-Santillán
R
R
Rubio-Arias
H O
HO
Méndez
J D
JD
D000328:Adult; D000975:Antioxidants / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006442:Glycated Hemoglobin; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008274:Magnesium / Q000097:blood; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D050177:Overweight / Q000097:blood*; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000097:blood; D014815:Vitamins / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology*
D000975:Antioxidants; D005979:Glutathione Peroxidase; D006442:Glycated Hemoglobin; D008274:Magnesium; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D014815:Vitamins; D024502:alpha-Tocopherol; C517652:hemoglobin A1c protein, human