title: Expression and characterization of two STAT isoforms from Sf9 cells.
abstract: In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
authors.affiliation: Department of Food and Nutrition, Hung-Kuang University, Taichung, Taiwan.
authors.forename: Maw-Sheng
authors.initials: MS
authors.lastname: Yeh
authors.forename: Chia-Hsiung
authors.initials: CH
authors.lastname: Cheng
authors.forename: Chih-Ming
authors.initials: CM
authors.lastname: Chou
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Hsu
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Chu
authors.forename: Gen-Der
authors.initials: GD
authors.lastname: Chen
authors.forename: Shui-Tsung
authors.initials: ST
authors.lastname: Chen
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Chen
authors.forename: Chang-Jen
authors.initials: CJ
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D050791:STAT Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D015533:Transcriptional Activation / Q000235:genetics; D048490:raf Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D050791:STAT Transcription Factors; D004247:DNA; D048490:raf Kinases