Halomonas profundus sp. nov., a new PHA-producing bacterium isolated from a deep-sea hydrothermal vent shrimp.
OBJECTIVE
The objective of the present work was to describe a new deep-sea, aerobic, mesophilic and heterotrophic bacterium, referenced as strain AT1214, able to produce polyhydroxyalkanoates (PHAs) under laboratory conditions. This bacterium was isolated from a shrimp collected nearby a hydrothermal vent located on the Mid-Atlantic Ridge.


RESULTS
This micro-organism, on the basis of the phenotypical features and genotypic investigations, can be clearly assigned to the Halomonas genus and the name of Halomonas profundus is proposed. Optimal growth occurred between 32 and 37 degrees C at a pH between 8 and 9 and at ionic strength between 20 and 30 g l(-1) of sea salts. The G + C content of DNA was 58.6%. This bacterium produced PHAs of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from different carbon sources.


CONCLUSIONS
The bacterium H. profundus produces PHA of 3HB and 3HV monomers from different carbon sources.


CONCLUSIONS
PHAs share physical and material properties that suggest them for application in various areas, and are considered as an alternative to nonbiodegradable plastics produced from fossil oils. In this study, we describe a new bacteria isolated from a deep-sea hydrothermal vent with the capability to produce polyesters of biotechnological interest.
Simon-Colin
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C
Institut Français de Recherche pour l'Exploitation de la Mer, Centre de Brest, BIOMAR/BMM, Plouzané, France. christelle.simon.colin@ifremer.fr
Raguénès
G
G
Cozien
J
J
Guezennec
J G
JG
D000818:Animals; D015373:Bacterial Typing Techniques; D033364:Decapoda / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D020632:Halomonas / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis / Q000737:chemistry; D012623:Seawater; D017550:Spectroscopy, Fourier Transform Infrared
D054813:Polyhydroxyalkanoates