Quantification of blood group A and B antibodies by flow cytometry using beads carrying A or B trisaccharides.
In the clinical management of patients receiving blood group ABO-incompatible organ allografts, it is of importance to determine the levels of blood group A and B antibodies before and after transplant. Currently used methods, which are mostly based on hemagglutination, are inexact and are associated with large intercenter variations. Here, we describe preliminary data from our efforts to establish a flow cytometry-based assay for the semiquantification of blood group A and B antibodies using beads carrying synthetic A or B trisaccharides. In agreement with previous investigations, blood group O individuals had greater levels of anti-A immunoglobulin G (IgG) than B individuals, whereas the levels of anti-A immunoglobulin M (IgM) were similar in sera from blood group O and B individuals.
Sundbäck
Maria
M
AbSorber AB, Stockholm, Sweden.
Grufman
Per
P
Teller
Joachim
J
Westphal
Fritz
F
Korchagina
Elena
E
Bovin
Nicolai
N
Holgersson
Jan
J
D000017:ABO Blood-Group System; D000327:Adsorption; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M / Q000737:chemistry; D007164:Immunosorbents / Q000737:chemistry; D033581:Stem Cell Transplantation; D014180:Transplantation / Q000379:methods*; D014312:Trisaccharides / Q000737:chemistry*
D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007164:Immunosorbents; D014312:Trisaccharides