title: Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
abstract: N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.
authors.affiliation: Department of Chemistry, South Kensington Campus, Imperial College, London, UK.
authors.forename: William P
authors.initials: WP
authors.lastname: Heal
authors.forename: Sasala R
authors.initials: SR
authors.lastname: Wickramasinghe
authors.forename: Paul W
authors.initials: PW
authors.lastname: Bowyer
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Holder
authors.forename: Deborah F
authors.initials: DF
authors.lastname: Smith
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Leatherbarrow
authors.forename: Edward W
authors.initials: EW
authors.lastname: Tate
mesh_terms: D000217:Acyltransferases / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D010963:Plasmodium falciparum / Q000201:enzymology; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins; D000217:Acyltransferases; C050480:glycylpeptide N-tetradecanoyltransferase