title: Halomonas sabkhae sp. nov., a moderately halophilic bacterium isolated from an Algerian sabkha.
abstract: A novel moderately halophilic bacterium belonging to the genus Halomonas was isolated from brine samples collected from Ezzemoul sabkha in north-eastern Algeria. The cells of strain 5-3(T) were Gram-negative, rod-shaped and non-motile. The strain was catalase- and oxidase-positive and produced an exopolysaccharide. Growth occurred at NaCl concentrations of 5-25% (optimum at 7.5%), at 30-50 degrees C (optimum at 37-40 degrees C) and at pH 6.0-9.0 (optimum at pH 7.5). The major fatty acids were C(12:0) 3-OH, C(16:1)omega7c/iso-C(15:0) 2-OH, C(16:0), C(18:1)omega7c and C(19:0)omega8c cyclo. The G+C content of the genomic DNA was 57.0 mol% (T(m)). The affiliation of strain 5-3(T) with the genus Halomonas was confirmed by 16S rRNA gene sequence comparisons. The most closely related species was Halomonas halmophila, which showed a 16S rRNA gene sequence similarity of 99.7%. However, the level of DNA-DNA relatedness between the novel isolate and the related Halomonas species was less than 31.4%. On the basis of the data from this polyphasic study, strain 5-3(T) represents a novel species of the genus Halomonas, for which the name Halomonas sabkhae sp. nov. is proposed. The type strain is 5-3(T) (=CECT 7246(T)=DSM 19122(T)=LMG 24084(T)).
authors.affiliation: Departamento de Microbiología, Facultad de Farmacia, Campus Universitario de Cartuja s/n 18071, Granada, Spain.
authors.forename: K
authors.initials: K
authors.lastname: Kharroub
authors.forename: M L
authors.initials: ML
authors.lastname: Jiménez-Pranteda
authors.forename: M
authors.initials: M
authors.lastname: Aguilera
authors.forename: A
authors.initials: A
authors.lastname: Boulahrouf
authors.forename: A
authors.initials: A
authors.lastname: Ramos-Cormenzana
authors.forename: M
authors.initials: M
authors.lastname: Monteoliva-Sánchez
mesh_terms: D000462:Algeria; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism*; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride