Characterization of a defective RNA derived from RNA 3 of the Y strain of cucumber mosaic virus.
A defective (D) RNA 3 naturally generated from the Y strain of cucumber mosaic virus (CMV-Y) was characterized using a biologically active cDNA clone. Sequencing of the clone revealed that the D RNA 3, named D RNA 3Yalpha, was derived from CMV-Y RNA 3 and contained a 10 nt deletion in the 5' untranslated region and a 162 nt deletion within the 3a open reading frame. Co-inoculation of D RNA 3Yalpha with CMV-Y derived from in vitro transcripts facilitated propagation of CMV-Y containing D RNA 3Yalpha in Nicotiana benthamiana or Nicotiana tabacum plants. CMV-Y, however, did not produce deletion mutants upon serial mechanical passages in the plants.
Takeshita
Minoru
M
Laboratory of Plant Pathology, Faculty of Agriculture, Division of Applied Genetic and Pest Management, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka, 812-8581, Japan. minorutk@agr.kyushu-u.ac.jp
Matsuo
Yuka
Y
Yoshikawa
Takaya
T
Suzuki
Masashi
M
Furuya
Naruto
N
Tsuchiya
Kenichi
K
Takanami
Yoichi
Y
D001483:Base Sequence; D017799:Cucumovirus / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology*; D010944:Plants / Q000821:virology; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D017384:Sequence Deletion; D014026:Nicotiana / Q000821:virology
D012367:RNA, Viral