title: [Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring].
abstract: OBJECTIVE
  To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood.
  
  
  METHODS
  This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multi-ple-reaction monitoring (MRM). After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode.
  
  
  RESULTS
  Identification was based on the compound's retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1 ng/mL to 20 ng/mL in blood.
  
  
  CONCLUSIONS
  The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.
authors.affiliation: Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Shen
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Shen
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
authors.forename: He-jian
authors.initials: HJ
authors.lastname: Wu
mesh_terms: D000157:Aconitine / Q000097:blood; D000470:Alkaloids / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D003078:Colchicine / Q000097:blood; D005554:Forensic Medicine; D006801:Humans; D012680:Sensitivity and Specificity; D013331:Strychnine / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D013331:Strychnine; D003078:Colchicine; D000157:Aconitine