title: High association of human papillomavirus infection with oral cancer: a case-control study.
abstract: BACKGROUND
  The aim of the present study was to determine the association of high-risk human papillomavirus (HR-HPV) in Mexican individuals with oral squamous cell carcinoma (OSCC) and their association with various risk factors.
  
  
  METHODS
  We designed a matched case-control study. Cases were individuals with newly diagnosed OSCC, age- and sex-matched with controls (1:4). Demographic and clinical data were obtained; also a self-administered questionnaire about sexual behavior was included. DNA from oral brushing was purified to amplify HPV-DNA through MY09/MY11 and GP5+/GP6+ primers and subsequently subjected to sequencing. Conditional regression models were built to calculate odds ratios (ORs) and 95% confidence intervals (CI).
  
  
  RESULTS
  Sixty two cases and 248 controls (53.2% males), median age 62 years (Q1-Q3=54-72 years) were included. HPV prevalence was 43.5% in cases and 17.3% in controls (HR-HPV: 37.1% cases, 9.7% controls). The most frequent types in cases were HPV-16 and HPV-18 (55.6 and 18.5%). The presence of HR-HPV was associated with OSCC (OR=6.2; 95% CI: 2.98-12.97) controlling for the most common risk factors. An interaction between smoking and drinking was detected, and family history of cancer was also significant (OR: 3.61; 95% CI=1.44-8.99). Early age at first sexual intercourse and large number of lifetime sexual partners showed an association with HR-HPV (p=0.019 and p=0.033, respectively).
  
  
  CONCLUSIONS
  Oral HR-HPV was strongly associated with OSCC, suggesting that HPV-16 and -18 are risk factors for oral cancer in Mexican patients. A significant association of tobacco and alcohol was confirmed. In addition, family history of cancer was associated with OSCC. The results underline the role of HPV in OSCC and its multifactorial etiology.
authors.affiliation: Universidad Autónoma Metropolitana, Mexico, D.F., Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Anaya-Saavedra
authors.forename: Velia
authors.initials: V
authors.lastname: Ramírez-Amador
authors.forename: Ma Esther
authors.initials: ME
authors.lastname: Irigoyen-Camacho
authors.forename: Claudia Ma
authors.initials: CM
authors.lastname: García-Cuellar
authors.forename: Miriam
authors.initials: M
authors.lastname: Guido-Jiménez
authors.forename: Rocío
authors.initials: R
authors.lastname: Méndez-Martínez
authors.forename: Alejandro
authors.initials: A
authors.lastname: García-Carrancá
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics; D052161:Human papillomavirus 18 / Q000235:genetics; D006801:Humans; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000453:epidemiology* / Q000235:genetics / Q000821:virology*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000235:genetics; D015995:Prevalence; D012189:Retrospective Studies; D012307:Risk Factors; D011795:Surveys and Questionnaires
chemical_list: D004279:DNA, Viral