title: Beneficial effects of hyperbaric oxygen pretreatment on massive hepatectomy model in rats.
abstract: BACKGROUND
  The purpose of this study was to investigate the impact of hyperbaric oxygen (HBO) pretreatment in massive hepatectomy model, a surrogate model of small-for-size graft, using rats.
  
  
  METHODS
  (Experiment I) Rats were divided into the following four groups: HBO (-), HBO-1D (day), HBO-3D, and HBO-5D. Samples were taken after the completion of HBO pretreatment, and the following parameters were evaluated: reverse transcription polymerase chain reaction and immunohistochemical staining for HSP 70 and HO-1; biochemical parameters; and liver weight to body weight ratio (Lw/Bw ratio). (Experiment II) Rats were divided into four groups as follows; 70% hepatectomy (Hx), 70% Hx-HBO, 90% Hx, and 90% Hx-HBO group. Samples were taken 12, 24, 48, and 72 hr after hepatectomy and the following parameters were investigated: biochemical analysis; Lw/Bw ratio; PCNA labeling index; and survival.
  
  
  RESULTS
  (Experiment I) The expression of HSP70 mRNA was significantly increased in the HBO-3D group compared with the HBO (-) group (P<0.05). HSP70- and HO-1-positive hepatocytes were significantly increased in the HBO-3D group compared with the HBO (-) group (P<0.05). (Experiment II) Transaminases were significantly decreased in both 70% and 90% Hx-HBO groups compared with Hx alone group (P<0.05). The Lw/Bw ratio and PCNA labeling index of the 90% Hx-HBO group were significantly increased compared with the 90% Hx group, 24, 48 and 72 hr after hepatectomy (P<0.05). The survival rate in the 90% Hx-HBO group was significantly higher than that in the 90% Hx group (P=0.01).
  
  
  CONCLUSIONS
  HBO pretreatment had beneficial effects in a massive hepatectomy model in rats via the induction of HSP70 and HO-1.
authors.affiliation: Department of Surgery, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Mori
authors.forename: Hisamitsu
authors.initials: H
authors.lastname: Shinohara
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kanemura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ikemoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Imura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morine
authors.forename: Toru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Yoshizumi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Shimada
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics; D006498:Hepatectomy / Q000379:methods*; D006931:Hyperbaric Oxygenation / Q000379:methods*; D008297:Male; D023421:Models, Animal; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D017392:Thiobarbituric Acid Reactive Substances; D051547:Heme Oxygenase-1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin