title: [Method of antibody analysis based on visual protein array].
abstract: OBJECTIVE
  To establish a novel method for antibody analysis based on visual protein microarray.
  
  
  METHODS
  The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.
  
  
  RESULTS
  The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007.
  
  
  CONCLUSIONS
  This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100037, China. yzhsd@163.com
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yu
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Liu
authors.forename: Ying-song
authors.initials: YS
authors.lastname: Wu
authors.forename: Jian-en
authors.initials: JE
authors.lastname: Gao
authors.forename: Qi-hong
authors.initials: QH
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Yin
authors.forename: Dan-ke
authors.initials: DK
authors.lastname: Xu
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000941:Antigens; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D005456:Fluorescent Dyes; D006967:Hypersensitivity; D007118:Immunoassay / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007136:Immunoglobulins / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles; D040081:Protein Array Analysis; D040901:Proteomics; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D000941:Antigens; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007073:Immunoglobulin E; D001710:Biotin