Chaperone mediated solubilization of 69-kDa recombinant maltodextrin glucosidase in Escherichia coli.
OBJECTIVE
To investigate the factors affecting expression and solubilization of Escherichia coli maltodextrin glucosidase in E. coli.


RESULTS
Expression level and solubilization of the recombinant E. coli maltodextrin glucosidase was studied in E. coli at different temperatures, in presence of overexpressed GroEL, GroES and externally supplemented glycerol. Aggregation of maltodextrin glucosidase in the cytoplasm was partially prevented by the co-expression of GroEL and GroES, and using externally supplemented glycerol or lowering the culture temperature. Co-expression of GroEL and GroES or simultaneous presence of overexpressed GroEL, GroES and externally supplemented glycerol together resulted significant increase of the activity of maltodextrin glucosidase. The growth rate of E. coli was inhibited by the formation of inclusion bodies whereas the presence of overexpressed GroEL, GroES alone or together with glycerol enhanced the growth rate of E. coli substantially.


CONCLUSIONS
The results indicated that lowering the temperature, use of GroEL, GroES and glycerol could be few controlling factors for the solubilization of recombinant aggregation-prone maltodextrin glucosidase in E. coli.


CONCLUSIONS
Our study could help in developing the strategy for enhancing the production of soluble industrial enzymes and finding the therapeutic agents against protein misfolding diseases.
Paul
S
S
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India. subhankar_paul@rediffmail.com
Chaudhuri
T K
TK
D019149:Bioreactors / Q000382:microbiology*; D018835:Chaperonin 10; D018834:Chaperonin 60; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005990:Glycerol / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000737:chemistry*; D007218:Industrial Microbiology; D018832:Molecular Chaperones; D017510:Protein Folding; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature
D018835:Chaperonin 10; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; D006026:Glycoside Hydrolases; C071233:malZ protein, E coli; D005990:Glycerol