Bioactive triterpene saponins from the roots of Phytolacca americana.
Five new triterpene saponins named phytolaccasaponins N-1 (1), N-2 (2), N-3 (3) N-4 (4), and N-5 (5) were isolated from the roots of Phytolacca americana together with seven known triterpene saponins (6-12). The structures of the five new saponins were established as shown in structures 1-5 on the basis of their spectroscopic data. The MDR-reversal activity of 1-12 was evaluated on the basis of the amount of calcein accumulated in MDR human ovarian cancer 2780 AD cells in the presence of each compound. The most effective compound was 8 (155% of control at 25 microg/mL).
Wang
Liyan
L
Graduate School of Science and Technology, Department of Chemistry and Chemical Engineering, Niigata University, Japan. wlydlm@yahoo.co.jp
Bai
Liming
L
Nagasawa
Takashi
T
Hasegawa
Toshiaki
T
Yang
Xiaoyang
X
Sakai
Jun-ichi
J
Bai
Yuhua
Y
Kataoka
Takao
T
Oka
Seiko
S
Hirose
Katsutoshi
K
Tomida
Akihiro
A
Tsuruo
Takashi
T
Ando
Masayoshi
M
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects; D053582:Interleukin-1alpha / Q000494:pharmacology; D015394:Molecular Structure; D029604:Phytolacca americana / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D053582:Interleukin-1alpha; D012503:Saponins; D014315:Triterpenes; D018799:Intercellular Adhesion Molecule-1