A novel approach for screening of new anti-Helicobacter pylori substances.
Gastric mucosal cell-derived L-lactic acid strongly enhances proliferation of Helicobacter pylori, and may contribute to the long-term colonization of H. pylori in the stomach. Therefore it is assumed that inhibitory substances active against L-lactic acid-dependent growth of H. pylori will be useful candidates as novel therapeutic agents for H. pylori infection. In this study, we developed a new assay system for screening anti-H. pylori substances, and baicalein and glycyrrhetinic acid were found as potent inhibitory substances against L-lactic acid-dependent H. pylori growth but not L-lactic acid-independent growth. The newly developed assay system described in this study also may facilitate the development of novel therapeutic agents for H. pylori infection.
Matsumoto
Tsukasa
T
The Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan.
Takahashi
Tetsufumi
T
Yamada
Haruki
H
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D044950:Flavanones / Q000494:pharmacology*; D005470:Fluorometry; D006034:Glycyrrhetinic Acid / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development; D019344:Lactic Acid / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D044950:Flavanones; D019344:Lactic Acid; C006680:baicalein; D006034:Glycyrrhetinic Acid