Lauric acid dependent enhancement in hepatic SCPx protein requires an insulin deficient environment.
Sterol carrier protein X (SCPx) is a peroxisomal protein with both lipid transfer and thiolase activity. Treating with the fatty acid, lauric acid, induced SCPx mRNA levels in rat liver and in rat hepatoma H4IIE cells but enhanced protein levels of SCPx and the thiolase produced as a post-translational modification of SCPx were only seen in H4IIE cells. Further investigation revealed that the presence of insulin can mask lauric acid effects on the SCPx gene especially at the protein level. These data are in agreement with the findings that diabetes, a medical condition characterized by high levels of fatty acids in an insulin deficient environment, enhances the hepatic expression of SCPx.
Lopez
Dayami
D
Department of Molecular Medicine, School of Basic Biomedical Sciences, University of South Florida, College of Medicine, Tampa, FL 33612, USA. dayami@moffitt.org
Niesen
Melissa
M
Bedi
Mohini
M
McLean
Mark P
MP
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007850:Lauric Acids / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D002352:Carrier Proteins; D007328:Insulin; D007850:Lauric Acids; C072087:sterol carrier proteins; C030358:lauric acid