Osteopontin gene polymorphism in association with systemic lupus erythematosus in Chinese patients.
BACKGROUND
Osteopontin (OPN) is one kind of cytokine which can play a number of roles in promoting activation of T lymphocyte, regulating balance between Th1 and Th2, participating in cell-induced immunologic response and stimulating B lymphocyte to express multi-clone antibodies. Some researches have showed that OPN may be involved in the pathogenesis of systemic lupus erythematosus (SLE). The aim of this study was to investigate possible association of a single nucleotide polymorphism (SNP) at position 9250 in exon 7 of the OPN gene (OPN gene 9250) with SLE in Chinese patients.


METHODS
Totally 158 patients (18 males and 140 females) fulfilled the revised criteria for SLE by the American College of Rheumatology in 1982 and 180 healthy volunteer controls (34 males and 146 females), all from the south of China, consented to participate in the study. OPN gene 9250 polymorphism was detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).


RESULTS
The frequency of TT genotype of the OPN gene 9250 was significantly lower (52.5% vs 70%, P < 0.05) and the frequency of TC genotype of the OPN gene 9250 was significantly higher (43.7% vs 29.4%, P < 0.05) in SLE patients than in controls. There were significant differences in OPN gene 9250 allele and phenotype frequencies between the SLE patients and controls (P < 0.05). When the SLE patients and controls were separated into men and women, significant differences of frequencies were noted in TT genotype, TC genotype and allele of the OPN gene 9250 in women (P < 0.05) but not in men (P > 0.05).


CONCLUSIONS
OPN gene 9250 polymorphism appears to be associated with susceptibility to SLE in Chinese Han ethnic population.
Xu
An-ping
AP
Department of Nephrology, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. anpxu@hotmail.com
Bai
Jie
J
Lü
Jun
J
Liang
Yan-yi
YY
Li
Jin-gao
JG
Lai
De-yuan
DY
Wan
Xia
X
Huang
Hu-hui
HH
D000293:Adolescent; D000328:Adult; D000368:Aged; D002681:China / Q000208:ethnology; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008181:Lupus Nephritis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D053495:Osteopontin