title: Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy.
abstract: Coronin-3 (coronin-1C), a homotrimeric F-actin binding protein, has been shown to be important for cell migration and brain morphogenesis. Here, we present for the first time a detailed analysis of the expression pattern of coronin-3 in human brain tumours and demonstrate that coronin-3 expression correlates with malignant phenotype in diffuse gliomas. In general, the expression of coronin-3 varies in different brain tumour entities. However, in diffuse gliomas, the number of coronin-3 expressing tumour cells correlates with the degree of malignancy. High-grade gliomas, such as anaplastic astrocytomas, anaplastic oligodendrogliomas, anaplastic oligoastrocytomas and glioblastomas, show high numbers of tumour cells positive for coronin-3, while diffuse low-grade gliomas, such as diffuse astrocytomas, oligodendrogliomas and oligoastrocytomas, exhibit low numbers of coronin-3-positive tumour cells. In order to explore and verify a contribution of coronin-3 to the malignant phenotype of diffuse gliomas, we employed an efficient shRNA-mediated coronin-3 knockdown in U373 and A172 human glioblastoma cells. Coronin-3 knockdown glioblastoma cells exhibited reduced levels of cell proliferation, cell motility and invasion into extracellular matrix compared to control cells. Together, our findings demonstrate evidence for a contribution of coronin-3 expression in the malignant progression of diffuse gliomas.
authors.affiliation: Department of Neuropathology, University of Bonn Medical Centre, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany.
authors.forename: Dr
authors.initials: D
authors.lastname: Thal
authors.forename: C-P
authors.initials: CP
authors.lastname: Xavier
authors.forename: A
authors.initials: A
authors.lastname: Rosentreter
authors.forename: S
authors.initials: S
authors.lastname: Linder
authors.forename: B
authors.initials: B
authors.lastname: Friedrichs
authors.forename: A
authors.initials: A
authors.lastname: Waha
authors.forename: T
authors.initials: T
authors.lastname: Pietsch
authors.forename: M
authors.initials: M
authors.lastname: Stumpf
authors.forename: Aa
authors.initials: A
authors.lastname: Noegel
authors.forename: Cs
authors.initials: C
authors.lastname: Clemen
mesh_terms: D001253:Astrocytes / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000473:pathology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D008840:Microfilament Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; C072061:coronin proteins; D020782:Matrix Metalloproteinases