title: Viscerotropic growth pattern of Leishmania tropica in BALB/c mice is suggestive of a murine model for human viscerotropic leishmaniasis.
abstract: Leishmania (L.) tropica is a causative agent of cutaneous leishmaniasis, and occasionally of visceral or viscerotropic leishmaniasis in humans. Murine models of Leishmania infection have been proven to be useful for elucidation of mechanisms for pathogenesis and immunity in leishmaniasis. The aim of this study was to establish a murine model for human viscerotropic leishmaniasis, and the growth pattern of L. tropica was studied in different tissues of BALB/c mice in order to find out whether the parasite visceralizes in this murine model. L. major was used as a control as this species is known to cause a progressive infection in BALB/c mice. L. tropica or L. major was injected into the footpad of mice, and thickness of footpad, parasite loads in different tissues, and the weight of the spleen and lymph node were determined at different intervals. Results showed that L. tropica visceralizes to the spleen and grows there while its growth is controlled in footpad tissues. Dissemination of L. tropica to visceral organs in BALB/c mice was similar to the growth patterns of this parasite in human viscerotropic leishmaniasis. The BALB/c model of L. tropica infection may be considered as a good experimental model for human diseases.
authors.affiliation: Immunology Department, Pasteur Institute of Iran, Tehran, Iran. mahmoudzadehh@yahoo.com
authors.forename: Hamid
authors.initials: H
authors.lastname: Mahmoudzadeh-Niknam
authors.forename: Simin Sadat
authors.initials: SS
authors.lastname: Kiaei
authors.forename: Davood
authors.initials: D
authors.lastname: Iravani
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005528:Foot / Q000469:parasitology; D006801:Humans; D018320:Leishmania major / Q000254:growth & development; D007895:Leishmania tropica / Q000254:growth & development*; D007896:Leishmaniasis / Q000469:parasitology*; D008198:Lymph Nodes / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size; D013154:Spleen / Q000469:parasitology / Q000473:pathology