Expression and DNA binding activity of the tomato transcription factor RIN (ripening inhibitor).
Recently, we have found that the accumulation of ripening inhibitor (RIN) protein increased gradually during tomato fruit ripening. Here, the recombinant protein was expressed in Escherichia coli and affinity-purified. The DNA binding activity of renatured RIN protein was tested by electrophoretic mobility shift assay. The results indicated that an optimal expression and purification system was suitable for obtaining active RIN with DNA binding activity.
Zhu
Hong-Liang
HL
Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Qinghua Donglu, Beijing, PR China.
Zhu
Ben-Zhong
BZ
Li
Ying-Cong
YC
Shao
Yi
Y
Wang
Xiao-Guang
XG
Xie
Yuan-Hong
YH
Chen
An-Jun
AJ
Luo
Jun-Jie
JJ
Jia
Xiao-Yan
XY
Luo
Yun-Bo
YB
D018744:DNA, Plant / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
D018744:DNA, Plant; D004268:DNA-Binding Proteins; D026161:MADS Domain Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors