title: HIV-1 strains identified in Brazilian blood donors: significant prevalence of B/F1 recombinants.
abstract: In the Brazilian HIV-1 epidemic subtypes B, C, and F1 are cocirculating in the high risk population groups, and there is a high prevalence of intersubtype recombinant forms. The dynamic nature of the HIV epidemic in Brazil led us to study HIV-1 subtypes present in HIV-infected blood donations collected from 2001 to 2003. Donations from 91 seropositive donors were evaluated. Genetic subtype was obtained for 88 specimens based on sequence analysis of gag p24, pol IN, and env gp41 IDR. HIV-1 subtype B was the predominant strain present in the donor population (73.9%). A significant prevalence of intersubtype recombinants of subtypes B and F1 was found (22.7%). Subtype C (1.1%) and F1 (2.3%) were rare. None of the B/F1 recombinants is CRF28_BF or CRF29_BF. The high level of unique B/F1 recombinant strains in this population demonstrates the dynamic and complex nature of the HIV epidemic in Brazil.
authors.affiliation: Abbott Diagnostics, Abbott Park, Illinois 60064, USA. catherine.brennan@abbott.com
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Brennan
authors.forename: Carlos
authors.initials: C
authors.lastname: Brites
authors.forename: Pierre
authors.initials: P
authors.lastname: Bodelle
authors.forename: Alan
authors.initials: A
authors.lastname: Golden
authors.forename: John
authors.initials: J
authors.lastname: Hackett
authors.forename: Vera
authors.initials: V
authors.lastname: Holzmayer
authors.forename: Priscilla
authors.initials: P
authors.lastname: Swanson
authors.forename: Ana
authors.initials: A
authors.lastname: Vallari
authors.forename: Julie
authors.initials: J
authors.lastname: Yamaguchi
authors.forename: Sushil
authors.initials: S
authors.lastname: Devare
authors.forename: Celia
authors.initials: C
authors.lastname: Pedroso
authors.forename: André
authors.initials: A
authors.lastname: Ramos
authors.forename: Roberto
authors.initials: R
authors.lastname: Badaro
mesh_terms: D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D005838:Genotype; D016655:HIV Core Protein p24 / Q000235:genetics; D015700:HIV Envelope Protein gp41 / Q000235:genetics; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D016655:HIV Core Protein p24; D015700:HIV Envelope Protein gp41; D012367:RNA, Viral; C514787:gp41 protein, Human immunodeficiency virus 1; C514799:p24 protein, Human Immunodeficiency Virus Type 1; D054302:pol Gene Products, Human Immunodeficiency Virus