Isolation and identification of newly isolated antagonistic Streptomyces sp. strain AP19-2 producing chromomycins.
A new antagonistic strain of actinomycete, designated AP19-2, was isolated from the feces of giant pandas inhabiting the Foping National Nature Reserve in China. Cultural characteristic studies strongly suggested that this strain is a member of the genus Streptomyces. The nucleotide sequence of the 16S rRNA gene of strain AP19-2 evidenced profound similarity (97-99%) with other Streptomyces strains. Two pure active molecules were isolated from a fermentation broth of Streptomyces sp. strain AP19-2 via extraction, concentration, silica gel G column chromatography, and HPLC. The chemical structures of the two related compounds (referred to as chromomycin A2 and chromomycin A3) were established on the basis of their Infrared spectra (IR), High Resolution Electrospray Ionization Mass Spectrometry (HR-ESI-MS), and Nuclear Magnetic Resonance (NMR) data, and by comparison with published data.
Wu
Xue-Chang
XC
Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, PR China. mblab@163.com
Chen
Wei-Feng
WF
Qian
Chao-Dong
CD
Li
Ou
O
Li
Ping
P
Wen
Yan-Ping
YP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002865:Chromomycins / Q000737:chemistry / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013302:Streptomyces / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D001503:Ursidae
D002865:Chromomycins; D012336:RNA, Ribosomal, 16S