title: Changes in PDGF expression in spared dorsal root ganglia and associated spinal dorsal horns in cats subjected to partial dorsal root ganglionectomy.
abstract: Changes in the platelet derived growth factor (PDGF) in the spared dorsal root ganglia (DRG) and associated spinal dorsal horns were evaluated in cats subjected to unilateral removal of L1-L5 and L7-S2 DRG, sparing the L6 DRG. The number of PDGF immunopositive neurons and protein expression decreased significantly in the spared DRG and associated dorsal horns of the L3 and L6 cord segments at 3 days post-operation (dpo). It bottomed to the lowest level at 7 dpo in the DRG, then returned to the control level at 14 dpo; while in the L6 dorsal horn, it rapidly increased at 7 dpo and exceeded the control level at 14 dpo. This showed a significant upregulation in the spared DRG and associated spinal dorsal horns, especially in the L6 cord segment following a transient decrease. Meanwhile, a significant upregulation of PDGF mRNA was also seen in L6 DRG and L3 and L6 dorsal horns at 3 dpo. The upregulation of the endogenous PDGF in the said structures indicated a potential role of this factor in spinal cord plasticity after partial dorsal root ganglia removal in cats.
authors.affiliation: Institute of Neurological Disease, West China Hospital, Sichuan University, Chengdu 610041 [corrected] China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Sun
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xu-Yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Lian-Shuang
authors.initials: LS
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002415:Cats; D005727:Ganglia, Spinal / Q000378:metabolism*; D015171:Ganglionectomy; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism*; D020671:Posterior Horn Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013116:Spinal Cord / Q000166:cytology*
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger