Structural studies of the O-polysaccharide chain from the lipopolysaccharide of symbiotically enhanced mutant Mlo-13 of Mesorhizobium loti NZP2213.
The O-specific polysaccharide (OPS) of Mesorhizobium loti mutant Mlo-13 was obtained by mild-acid degradation of the lipopolysaccharide and studied by sugar and ethylation analyses, Smith degradation, as well as (1)H and (13)C NMR spectroscopy. The O-polysaccharide has a trisaccharide repeating unit composed of 6-deoxy-L-talose (L-6dTal), 4-O-acetyl-6-deoxy-L-talose (L-6dTal-4Ac) and L-rhamnose residues. An analysis of NMR spectra revealed that the rhamnose residue was substituted nonstoichiometrically with O-methyl group at C-3. The following structure of the repeating unit of the Mlo-13 OPS was established: [structure: see text].
Turska-Szewczuk
Anna
A
Department of General Microbiology, M. Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland.
Palusinska-Szysz
Marta
M
Russa
Ryszard
R
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007783:Lactones; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009154:Mutation; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D012210:Rhamnose; D012231:Rhizobium / Q000737:chemistry*
C510913:L-talo-gamma-lactone; D007783:Lactones; D008070:Lipopolysaccharides; D019081:O Antigens; D012210:Rhamnose