An ionically crosslinked hydrogel containing vancomycin coating on a porous scaffold for drug delivery and cell culture.
The aim of this study was to prepare and characterize a scaffold with an ionically crosslinked hydrogel coating layer containing a water-soluble drug, vancomycin, via a novel drug loading method for sustained drug delivery and surface modification. The poly(D,L-lactide acid) (PDLLA)/biphasic calcium phosphate (BCP) scaffold with a highly inter-connected porous structure was fabricated by a particle-leaching/thermally induced phase separation (TIPS) method. The pre-vacuumized scaffold was immersed into an alginate/vancomycin solution. Following impregnation by the solution, the scaffold was removed and immersed in a CaCl(2) solution for 30 min to allow gelation of the alginate solution. In this way, the drug was not exposed to organic solvents or detrimental temperature conditions and it could avoid loss of drug during the leaching process. The water contact angles of the scaffold surface decreased after being coated with the hydrogel. The in vitro drug release profile showed sustained release properties which were influenced by the alginate concentration and the dissolution medium. A standardized bacterial assay showed that the drug was still active after association with the scaffold by this gentle method of drug loading. The in vitro osteoblast culture experiments confirmed the biocompatibility of the scaffold for attachment and proliferation of osteoblasts.
Zhang
Li Fang
LF
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu, PR China.
Yang
De Juan
DJ
Chen
He Chun
HC
Sun
Rui
R
Xu
Liang
L
Xiong
Zuo Chun
ZC
Govender
Thirumala
T
Xiong
Cheng Dong
CD
D000464:Alginates / Q000008:administration & dosage; D000818:Animals; D002130:Calcium Phosphates / Q000008:administration & dosage; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength; D016503:Drug Delivery Systems; D020723:Glucuronic Acid / Q000008:administration & dosage; D006603:Hexuronic Acids / Q000008:administration & dosage; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage*; D019344:Lactic Acid / Q000008:administration & dosage; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage; D016062:Porosity; D051381:Rats; D012995:Solubility; D014640:Vancomycin / Q000008:administration & dosage* / Q000737:chemistry; D017155:Wettability
D000464:Alginates; D002130:Calcium Phosphates; D006603:Hexuronic Acids; D011091:Polyesters; D011108:Polymers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D019344:Lactic Acid; C033616:poly(lactide); D014640:Vancomycin; D020723:Glucuronic Acid; C020243:calcium phosphate