Caenorhabditis elegans DYF-11, an orthologue of mammalian Traf3ip1/MIP-T3, is required for sensory cilia formation.
Cilia and flagella play critical roles in cell motility, development and sensory perception in animals. Formation and maintenance of cilia require a conserved protein transport system called intraflagellar transport (IFT). Here, we show that Caenorhabditis elegans dyf-11 encodes an evolutionarily conserved protein required for cilium biogenesis. dyf-11 is expressed in most of the ciliated neurons and is regulated by DAF-19, a crucial transcription factor for ciliary genes in C. elegans. dyf-11 mutants exhibit stunted cilia, fluorescent dye-filling defects (Dyf) of sensory neurons, and abnormal chemotaxis (Che). Cell- and stage-specific rescue experiments indicated that DYF-11 is required for formation and maintenance of sensory cilia in cell-autonomous manner. Fluorescent protein-tagged DYF-11 localizes to cilia and moves antero- and retrogradely via IFT. Analysis of DYF-11 movement in bbs mutants further suggested that DYF-11 is likely associated with IFT complex B. Domain analysis using DYF-11 deletion constructs revealed that the coiled-coil region is required for proper localization and ciliogenesis. We further show that Traf3ip1/MIP-T3, the mammalian orthologue of DYF-11, localizes to cilia in the MDCK renal epithelial cells.
Kunitomo
Hirofumi
H
Molecular Genetics Research Laboratory, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Iino
Yuichi
Y
D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology* / Q000648:ultrastructure; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002633:Chemotaxis / Q000502:physiology; D002923:Cilia / Q000502:physiology; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000502:physiology; D009154:Mutation; D009475:Neurons, Afferent / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D029742:Caenorhabditis elegans Proteins; C410596:DAF-19 protein, C elegans; C529577:DYF-11 protein, C elegans; D008869:Microtubule-Associated Proteins; D014157:Transcription Factors