[The Pro12Ala polymorphism of the peroxisome proliferator-activated receptor gamma and immunological processes in patients with type 2 diabetes and insulin resistance].
BACKGROUND
The peroxisome proliferator-activated receptor gamma (PPARgamma), a transcriptor factor, regulates immunological and metabolic processes, which are important for carbohydrate and lipid metabolism. Various polymorphic forms of PPARgamma may promote diabetes mellitus and diabetic complications.


OBJECTIVE
The assessment of TNFalpha gene expression in peripheral blood monocytes, serum TNFalpha concentration and anti-GAD and ICA antibodies in relation to the polymorphism Pro12Ala in patients with 2 diabetes.


METHODS
58 patients with type 2 diabetes (average age 59.0 +/- 11 years) and 18 healthy people were examined. The Pro12Ala polymorphism of PPARy gene were assessed using mini-sequence technic SnaPshot [ABIPRISM-310]. The TNFalpha gene expression were estimated using real-time PCR [Applied Bio-systems]. The TNFalpha concentration [Quantikin Immunoassay, R&D Systems] and ICA and GAD antibodies [immunofluorescence method, DRG] were evaluated in venous blood.


RESULTS
A heterozygotous genotype Pro12Ala was estimated in 32 patients and a homozygotous genotype Pro12Pro in 21. Only 6 patients were positive for GAD antibodies and only 6 patients for ICA antibodies. The TNFalpha concentration in serum and the TNFalpha gene expression in monocytes did not refer to the Pro12ala polymorphism of PPARy and neither to antibodies.


CONCLUSIONS
1) The TNFalpha concentration in serum and the TNFalpha gene expression in monocytes do not refer to the Pro12ala polymorphism of PPARgamma in patients with type 2 diabetes. 2) The Pro12Ala genotype do not influence autoimmunologic processes of diabetes.
Gacka
Małgorzata
M
Katedra i Klinika Angiologii, Nadciśnienia Tetniczego i Diabetologii AM, Wrocław. magacka@poczta.onet.pl
Bednarska-Chabowska
Dorota
D
Dobosz
Tadeusz
T
Szymaniec
Stanisław
S
Jakobsche
Urszula
U
Lebioda
Arleta
A
Adamiec
Rajmund
R
D000368:Aged; D019943:Amino Acid Substitution; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000235:genetics* / Q000276:immunology*; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006442:Glycated Hemoglobin; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D047495:PPAR gamma / Q000235:genetics* / Q000276:immunology*; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D006442:Glycated Hemoglobin; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha