Biocompatibility of poly(D,L-lactide-co-glycolide) nanoparticles conjugated with alendronate.
Nanoparticles made of a conjugate of poly(D,L-lactide-co-glycolide) with alendronate (PLGA-ALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30-35%. PLGA-ALE NPs size, evaluated by photon correlation spectroscopy, was 198.7+/-0.2 nm. Haemocompatibility studies using different concentrations of PLGA-ALE NPs did not show any significant effect on haemolysis, leukocyte number, platelet activation, APTT and complement consumption, in comparison with blood incubated with phosphate buffered saline (PBS). A significant reduction of the prothrombin activity was demonstrated after incubation with 560 microg/ml of PLGA-ALE NPs; a significant increase was observed at the highest dilutions. The viability of human umbilical vein endothelial cells and bone marrow stromal cells (BMSC), evaluated through the neutral red test, was not affected by PLGA-ALE NPs. There were no significant differences in cell-associated alkaline phosphatase between BMSC incubated with PLGA-ALE NP- and PBS-added media. These results demonstrated that PLGA-ALE NPs had an acceptable degree of blood compatibility and were not cytotoxic; therefore, they may be considered suitable for intravenous administration.
Cenni
Elisabetta
E
Laboratorio di Fisiopatologia degli Impianti Ortopedici, Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy. elisabetta.cenni@ior.it <elisabetta.cenni@ior.it>
Granchi
Donatella
D
Avnet
Sofia
S
Fotia
Caterina
C
Salerno
Manuela
M
Micieli
Dorotea
D
Sarpietro
Maria G
MG
Pignatello
Rosario
R
Castelli
Francesco
F
Baldini
Nicola
N
D019386:Alendronate / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D007958:Leukocyte Count; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D015539:Platelet Activation / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000469:Alkaline Phosphatase; D019386:Alendronate