Hip, an HP1-interacting protein, is a haplo- and triplo-suppressor of position effect variegation.
The Drosophila heterochromatin protein 1 (HP1) regulates epigenetic gene silencing and heterochromatin formation by promoting and maintaining chromatin condensation. Here we report the identification and characterization of an HP1-interacting protein (Hip). Hip interacts with HP1 in vitro and is associated with HP1 in vivo. This interaction is mediated by at least three independent but similar HP1-binding modules of the Hip protein. Hip and HP1 completely colocalize in the pericentric heterochromatin, and both haplo- and triplo-dosage mutations act as dominant suppressors of position effect variegation. These findings identify a player in heterochromatinization and suggest that Hip cooperates with HP1 in chromatin remodeling and gene silencing.
Schwendemann
Alexander
A
Institut für Biologie, Freie Universität Berlin, Arnimallee 7, 14195 Berlin, Germany. aschwend@genetik.fu-berlin.de
Matkovic
Tanja
T
Linke
Christian
C
Klebes
Ansgar
A
Hofmann
Annemarie
A
Korge
Günter
G
D000595:Amino Acid Sequence; D000818:Animals; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology*; D005799:Genes, Dominant; D006570:Heterochromatin / Q000378:metabolism; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D020798:Two-Hybrid System Techniques
D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D006570:Heterochromatin; C452193:heterochromatin protein 1, Drosophila; D000090266:Chromobox Protein Homolog 5