title: [X-ray irradiation enhances tumor necrosis factor receptor p55 expression in human colorectal cancer cells and inhibits the release of its soluble form in vitro].
abstract: OBJECTIVE
  To investigate the effects of X ray on human colorectal cancer cells for their tumor necrosis factor receptor-p55 (TNFR-p55) expression and release of soluble soluble TNFR-p55 (sTNFR-p55) in vitro.
  
  
  METHODS
  The protein expression of TNFR-p55 in Lovo cells exposed to X-ray was detected using immunohistochemistry, and enzyme-linked immunosorbent assay was used to examine the levels of sTNFR-p55 in the supernatants of the cell culture. The cell apoptosis of the exposed cells was analyzed with flow cytometry, and the changes in cell morphology were observed microscopically.
  
  
  RESULTS
  X-ray exposure of cells resulted in a strong increase in TNFR-p55 expression of (P<0.01) and LoVo cell apoptosis (P<0.05). The levels of sTNFR-p55 in the supernatant of cells with X-ray exposure was significantly lowered in comparison with the levels before exposure (P<0.01). Optical microscopy showed that the exposed LoVo cells shrank and became spherical with cytoplasmic condensation and nuclear pyknosis.
  
  
  CONCLUSIONS
  X-ray exposure can induce LoVo cell apoptosis by increasing TNFR-p55 expression on the cell membrane and inhibiting the release of sTNFR-p55 in the supernatants.
authors.affiliation: Department of Imaging, Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China. gzgaolei@126.com
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Lan
authors.initials: L
authors.lastname: Bai
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Shi
authors.forename: Qing-zhen
authors.initials: QZ
authors.lastname: Nan
authors.forename: Zhan-bin
authors.initials: ZB
authors.lastname: Mao
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics / Q000378:metabolism*; D053319:Tumor Necrosis Factor Decoy Receptors / Q000235:genetics / Q000378:metabolism*; D014965:X-Rays
chemical_list: D047888:Receptors, Tumor Necrosis Factor, Type I; D053319:Tumor Necrosis Factor Decoy Receptors; C069521:recombinant human tumor necrosis factor-binding protein-1