Observing fibrillar assemblies on scrapie-infected cells.
The infectious agent in prion diseases is an aberrant-folded isoform of the cellular prion protein (PrPC). This scrapie-related prion protein (PrPSc) has an increased beta-sheet content, is detergent insoluble and proteinase K resistant, and accumulates in prion-infected organisms and cells. In vitro, PrPSc self-aggregates into amyloid fibrils. However, there is no direct experimental proof for the occurrence of PrPSc-containing fibrils in vivo or in cell cultures. Applying atomic force microscopy (AFM) to scrapie-infected mouse neuroblastoma (ScN2a) cells, we discovered growing patch-like assemblies of amyloid-like fibrillar structures on the cell surfaces. Immunofluorescence and AFM images showed heterogeneous accumulation and aggregation of PrPSc in ScN2a cell cultures. The percentage of cells having characteristic fibrils on their surface increased with time after scrapie infection. These endogeneous fibrils had lengths from 0.5 to 3 microm and protruded from the cell surface by 108 +/- 30 nm, and thus resembled the heterogeneous shapes and networks of in vitro prepared amyloid fibrils.
Wegmann
Susanne
S
Center of Biotechnology, University of Technology, Dresden, Germany.
Miesbauer
Margit
M
Winklhofer
Konstanze F
KF
Tatzelt
Jörg
J
Muller
Daniel J
DJ
D000682:Amyloid / Q000648:ultrastructure*; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D051379:Mice; D018625:Microscopy, Atomic Force; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology; D018622:PrP 27-30 Protein / Q000737:chemistry / Q000378:metabolism; D011328:Prions / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D017510:Protein Folding; D012608:Scrapie / Q000473:pathology*
D000682:Amyloid; D011328:Prions; D018622:PrP 27-30 Protein