Immobilization of Galphimia glauca plant cell suspensions for the production of enhanced amounts of Galphimine-B.
We tested the capacity of Galphimia glauca cells to produce galphimine-B (G-B) when under the effects of a two-stage culture system: cell immobilization in Ca2+-alginate beads and culture scale-up from shake-flask to two different types of bioreactor (stirred and airlift). In the shake-flask culture, using optimum media for cell growth (first stage) and G-B production (second stage), the G-B yield was similar in both immobilised and free cells. However, while the free cells accumulated G-B within cytoplasmatic compartments, where it could not be recovered without cell disruption, immobilized cells excreted up to 100 % of the G-B produced. Immobilized cells grown in bioreactors running for 14 days with growth medium and an additional 26 days with production medium in batch mode showed a high G-B yield. The stirred bioreactor was the most efficient with a G-B content in the culture medium of 1381 microg.L (-1) at day 24 of culture.
Osuna
Lidia
L
Facultad de Farmacia, Departamento de Fisiología Vegetal, Universidad de Barcelona, Barcelona, Spain. osunalidia@yahoo.com
Moyano
Elisabeth
E
Mangas
Susana
S
Bonfill
Mercè
M
Cusidó
Rosa M
RM
Piñol
M Teresa
MT
Zamilpa
Alejandro
A
Tortoriello
Jaime
J
Palazón
Javier
J
D019149:Bioreactors; D003470:Culture Media; D031582:Galphimia / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008517:Phytotherapy; D014315:Triterpenes / Q000378:metabolism*
D003470:Culture Media; D014315:Triterpenes; C084708:galphimine B