title: Direct electrochemistry of glucose oxidase and biosensing for glucose based on boron-doped carbon nanotubes modified electrode.
abstract: Due to their unique physicochemical properties, doped carbon nanotubes are now extremely attractive and important nanomaterials in bioanalytical applications. In this work, selecting glucose oxidase (GOD) as a model enzyme, we investigated the direct electrochemistry of GOD based on the B-doped carbon nanotubes/glassy carbon (BCNTs/GC) electrode with cyclic voltammetry. A pair of well-defined, quasi-reversible redox peaks of the immobilized GOD was observed at the BCNTs based enzyme electrode in 0.1M phosphate buffer solution (pH 6.98) by direct electron transfer between the protein and the electrode. As a new platform in glucose analysis, the new glucose biosensor based on the BCNTs/GC electrode has a sensitivity of 111.57 microA mM(-1)cm(-2), a linear range from 0.05 to 0.3mM and a detection limit of 0.01mM (S/N=3). Furthermore, the BCNTs modified electrode exhibits good stability and excellent anti-interferent ability to the commonly co-existed uric acid and ascorbic acid. These indicate that boron-doped carbon nanotubes are the good candidate material for the direct electrochemistry of the redox-active enzyme and the construction of the related enzyme biosensors.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Deng
authors.forename: Jinhua
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Chen
authors.forename: Chunhui
authors.initials: C
authors.lastname: Xiao
authors.forename: Lihua
authors.initials: L
authors.lastname: Nie
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001895:Boron / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D037742:Nanotubes, Carbon; D005949:Glucose Oxidase; D005947:Glucose; D001895:Boron