Simultaneous dynamic optical and electrical properties of endothelial cell attachment on indium tin oxide bioelectrodes.
This study quantifies the dynamic attachment and spreading of porcine pulmonary artery endothelial cells (PPAECs) on optically thin, indium tin oxide (ITO) biosensors using simultaneous differential interference contrast microscopy (DICM) and electrical microimpedance spectroscopy. A lock-in amplifier circuit monitored the impedance of PPAECs cultivated on the transparent ITO bioelectrodes as a function of frequency between 10 Hz and 100 kHz and as a function of time, while DICM images were simultaneously acquired. A digital image processing algorithm quantified the cell-covered electrode area as a function of time. The results of this study show that the fraction of the cell-covered electrode area is in qualitative agreement with the electrical impedance during the attachment phase following the cell settling on the electrode surface. The possibility of several distinctly different states of electrode coverage and cellular attachment giving rise to similar impedance signals is discussed.
Choi
Chang K
CK
University of Tennessee, Department of Mechanical, Aerospace and Biomedical Engineering, Knoxville, Tennessee 37996, USA.
English
Anthony E
AE
Kihm
Kenneth D
KD
Margraves
Charles H
CH
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000706:statistics & numerical data; D002448:Cell Adhesion; D017097:Electric Impedance; D004566:Electrodes; D042783:Endothelial Cells / Q000166:cytology*; D007091:Image Processing, Computer-Assisted; D008857:Microscopy, Interference / Q000379:methods / Q000706:statistics & numerical data; D055095:Optics and Photonics / Q000295:instrumentation*; D013552:Swine; D017971:Tin Compounds
D017971:Tin Compounds; C109984:indium tin oxide