Association of MSX1 with nonsyndromic cleft lip and palate in a Colombian population.
OBJECTIVE
To evaluate the association between MSX1 CA polymorphism and nonsyndromic cleft lip with or without cleft palate (CL+/-P) in a group of patients from Operation Smile Colombia.


METHODS
Four alleles from MSX1 CA microsatellite sequence were analyzed. Polymerase chain reaction was carried out. The amplifications were performed by Short Tandem Repeats (STRs) in ABI PRISM 310 genetic analyzer. Chi-square and odds ratio tests were used to determine the association between genotype frequencies and the risk to the cleft lip/palate in a Colombian group population.


METHODS
Operation Smile Colombia.


METHODS
Ninety-four affected patients (49 men and 45 women with CL+/-P) and 93 control individuals (43 men and 50 women).


RESULTS
A significant statistical difference (p<.0106) was found between the patients who carried allele 3 and CL+/-P. In addition, allele 4 (heterozygous and homozygous form) was the most frequent in CL+/-P (74%) patients and in the control group (82%).


CONCLUSIONS
These findings show a positive association between the MSX1 CA polymorphism and CL+/-P in a Colombian group population.
Otero
Liliana
L
Pontificia Universidad Javeriana, Bogotá, Colombia. lotero@javeriana.edu.co
Gutiérrez
Sandra
S
Cháves
Margarita
M
Vargas
C
C
Bérmudez
Luis
L
D019943:Amino Acid Substitution; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D003105:Colombia; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D051957:MSX1 Transcription Factor / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide
D051957:MSX1 Transcription Factor