Controlled cavitation-cell interaction: trans-membrane transport and viability studies.
Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance gamma is defined by gamma = h/R(max), with h being the initial distance and R(max) being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of gamma approximately 0.65. In contrast, the maximum of the molecular uptake is found when gamma approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.
Dijkink
Rory
R
Physics of Fluids, Faculty of Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
Le Gac
Séverine
S
Nijhuis
Erwin
E
van den Berg
Albert
A
Vermes
István
I
Poot
André
A
Ohl
Claus-Dieter
CD
D001693:Biological Transport, Active / Q000502:physiology / Q000528:radiation effects; D002448:Cell Adhesion / Q000502:physiology* / Q000528:radiation effects; D002450:Cell Communication / Q000502:physiology* / Q000528:radiation effects; D002462:Cell Membrane / Q000502:physiology* / Q000528:radiation effects; D002470:Cell Survival / Q000502:physiology* / Q000528:radiation effects; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers