Cellular location of KMP-11 protein in Trypanosoma rangeli.
We describe the localization of the KMP-11 protein in the Trypanosoma rangeli parasite determined by immunoelectron microscopy using a monoclonal antibody generated against the Trypanosoma cruzi KMP-11 protein. The data reported herein show that the T. rangeli KMP-11 protein is mainly accumulated in the parasite cytoplasm, the coat, the flagellum, and the flagellar pocket. The high degree of sequence homology between the KMP-11 proteins from both parasites suggests that the KMP-11 protein from T. rangeli, like that of T. cruzi, could also be associated with the parasite cytoskeleton.
Diez
Hugo
H
Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Bogotá, Colombia.
Sarmiento
Ladys
L
Caldas
María Leonor
ML
Montilla
Marleny
M
Thomas
María del Carmen
Mdel C
Lopez
Manuel Carlos
MC
Puerta
Concepción
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000276:immunology*; D005407:Flagella; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology*; D016253:Microscopy, Immunoelectron / Q000379:methods; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology*; D014345:Trypanosoma / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins