Inhibitory and excitatory perigenital-to-bladder spinal reflexes in the cat.
This study revealed that in awake chronic spinal cord-injured (SCI) cats reflexes from perigenital skin area to the bladder can be either inhibitory or excitatory. Electrical perigenital stimulation at frequencies between 5 and 7 Hz significantly inhibited large-amplitude rhythmic reflex bladder activity, whereas frequencies between 20 and 40 Hz induced large-amplitude bladder contractions even at low bladder volumes when reflex bladder activity was absent. Both inhibitory and excitatory effects were enhanced as the stimulation intensity increased (5-30 V, 0.2-ms pulse width). During cystometrograms, the inhibitory stimulation (7 Hz) significantly increased the micturition volume threshold 35 +/- 13% above the control volume, while the excitatory stimulation (30 Hz) significantly reduced the threshold 21 +/- 3%. Mechanical perigenital stimulation applied by repeated light stroking of the perigenital skin with a cotton swab only induced an excitatory effect on the bladder. Both electrical and mechanical perigenital stimuli induced large-amplitude (>30 cm H(2)O) bladder contractions that were relatively consistent over a range of bladder volumes (10-90% of the capacity). However, the excitatory electrical stimulation only induced bladder contractions lasting on average 42.2 +/- 3.9 s, but the mechanical stimulation induced bladder contractions that lasted as long as the stimulation continued (2-3 min). Excitatory electrical or mechanical perigenital stimulation also induced poststimulus voiding. The ability to either inhibit or excite the bladder by noninvasive methods could significantly transform the current clinical management of bladder function after SCI.
Tai
Changfeng
C
Department of Urology, University, of Pittsburgh, Pittsburgh, PA 15261, USA. cftai@pitt.edu
Shen
Bing
B
Wang
Jicheng
J
Chancellor
Michael B
MB
Roppolo
James R
JR
de Groat
William C
WC
D000818:Animals; D002415:Cats; D004558:Electric Stimulation; D005260:Female; D005836:Genitalia, Female / Q000294:innervation*; D012018:Reflex / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology; D013119:Spinal Cord Injuries / Q000150:complications; D001743:Urinary Bladder / Q000294:innervation / Q000502:physiology*; D014549:Urinary Incontinence / Q000209:etiology / Q000628:therapy; D014554:Urination / Q000502:physiology*