Expression of fibroblast growth factor 10 and its receptor, fibroblast growth factor receptor 2B, in the bovine corpus luteum.
There is evidence that several fibroblast growth factors (FGFs) are involved in growth and development of the corpus luteum (CL), but many FGFs have not been investigated in this tissue, including FGF10. The objective of this study was to determine if FGF10 and its receptor (FGFR2B) are expressed in the CL. Bovine CL were collected from an abattoir and classed as corpus hemorrhagica (stage I), developing (stage II), developed (stage III), and regressed (stage IV) CL. Expression of FGF10 and FGFR2B mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). Both genes were expressed in bovine CL, and FGF10 expression did not differ between stages of CL development. FGF10 protein was localized to large and small luteal cells by immunohistochemistry. FGFR2B expression was approximately threefold higher in regressed compared to developing and developed CL (P < 0.05). To determine if FGF10 and FGFR2B expression is regulated during functional luteolysis, cattle were injected with PGF2alpha and CL collected at 0, 0.5, 2, 4, 12, 24, 48, and 64 hr thereafter (n = 5 CL/time point), and mRNA abundance was measured by real-time RT-PCR. FGF10 mRNA expression did not change during functional luteolysis, whereas FGFR2B mRNA abundance decreased significantly at 2, 4, and 12 hr after PGF2alpha, and returned to pretreatment levels for the period 24-64 hr post-PGF2alpha. These data suggest a potential role for FGFR2B signaling during structural luteolysis in bovine CL.
Castilho
A C
AC
Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, São Paulo, Brasil. anthony.castilho@ig.com.br
Giometti
I C
IC
Berisha
B
B
Schams
D
D
Price
C A
CA
Amorim
R L
RL
Papa
P C
PC
Buratini
J
J
D000818:Animals; D002417:Cattle; D003338:Corpus Luteum / Q000166:cytology / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D007150:Immunohistochemistry; D003341:Luteolysis / Q000187:drug effects / Q000502:physiology*; D010120:Oxytocics / Q000494:pharmacology; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects*; D013997:Time Factors
D051526:Fibroblast Growth Factor 10; D010120:Oxytocics; D015237:Dinoprost; D051497:Receptor, Fibroblast Growth Factor, Type 2