title: Opisthorchis viverrini: efficacy and tegumental alterations following administration of tribendimidine in vivo and in vitro.
abstract: The tegumental changes in adult Opisthorchis viverrini induced by tribendimidine were analyzed by scanning electron microscopy (SEM). We exposed O. viverrini to tribendimidine at a concentration of 10 microg/ml for 4 h. In addition, hamsters were treated with a single 400 mg/kg oral dose of tribendimidine and flukes were recovered from the bile ducts 24, 48, 72, and 96 h post-treatment. SEM analysis of the flukes incubated in vitro showed only mild damage of the tegument. Twenty-four hours post-treatment of hamsters, extensive disruption such as sloughing, furrowing, or blebbing of the tegument was evident, which did not increase in severity 48-72 h post-treatment. Ninety-six hours after tribendimidine administration, the majority of flukes had been expelled. A single 400 mg/kg oral dose of tribendimidine administered to O. viverrini-infected hamsters yielded a worm burden reduction of 63.0%. In conclusion, our experiments confirm that tribendimidine possesses interesting trematocidal properties, which warrant additional investigations and provide further data for subsequent clinical trials.
authors.affiliation: Department of Medical Parasitology and Infection Biology, Swiss Tropical Institute, P.O. Box, CH-4002 Basel, Switzerland. jennifer.keiser@unibas.ch
authors.forename: Jennifer
authors.initials: J
authors.lastname: Keiser
authors.forename: Jürg
authors.initials: J
authors.lastname: Utzinger
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Xiao
authors.forename: Peter
authors.initials: P
authors.lastname: Odermatt
authors.forename: Smarn
authors.initials: S
authors.lastname: Tesana
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000627:therapeutic use; D006224:Cricetinae; D008855:Microscopy, Electron, Scanning; D009889:Opisthorchiasis / Q000188:drug therapy* / Q000469:parasitology; D009891:Opisthorchis / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure*; D021261:Parasitic Sensitivity Tests; D010655:Phenylenediamines / Q000008:administration & dosage / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D010655:Phenylenediamines; C056017:tribendimidine