Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for Light Cycler 1.2 instrument.
OBJECTIVE
Commercial TaqMan real-time PCR reagent was modified and applied on Light Cylcer 1.2 for quantifying HIV-1 RNA in plasma and compared with the reference method; COBAS AmpliPrep/COBAS Amplicor HIV-1 monitor test version 1.5.


METHODS
Three hundred and eight frozen and fresh plasma samples were used for evaluation. Sequential specimens were also tested for follow-up cases.


RESULTS
The correlation between HIV-1 RNA values obtained by reference and modified method with automated and manual sample preparation were significant with r = 0.916 and 0.908 (p < 0.001, p < 0.001) respectively with similar agreement log of mean bias (0.5 versus 0.48). High degree of correlation and agreement were observed between the assays in blind fresh plasma, r = 0.953 (p < 0.001) with 0.15 log difference in HIV-1 RNA level. Among follow-up samples, both methods gave 100% concordant results.


CONCLUSIONS
This modified protocol provided evidence for using modified commercial real-time PCR reagent for HIV-1 RNA quantitative detection as a monitoring tool for HIV/AIDS patients in Thailand.
Uttayamakul
Sumonmal
S
Bamrasnaradura Infectious Disease Institute, Department ofDisease Control, Ministry ofPublic Health, Nonthaburi, Thailand. sumonmal@health.moph.go.th
Likanonsakul
Sirirat
S
Sutthent
Ruengpung
R
Chaovavanich
Achara
A
D015658:HIV Infections / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D015497:HIV-1; D006801:Humans; D010865:Pilot Projects; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D012313:RNA / Q000032:analysis*; D012016:Reference Values; D013785:Thailand; D019562:Viral Load
D012313:RNA