title: Enterobacter pulveris sp. nov., isolated from fruit powder, infant formula and an infant formula production environment.
abstract: Six Gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped isolates were obtained from fruit powder (n=3), infant formula (n=2) and an infant formula production environment (n=1) and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB gene sequence analysis allocated the isolates to the family Enterobacteriaceae. The highest rpoB gene sequence similarities (91.2-95.8%) were obtained with Enterobacter helveticus, Enterobacter radicincitans, Enterobacter turicensis and Enterobacter sakazakii and the phylogenetic branch formed by these species was supported by a high bootstrap value. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by their ability to utilize melibiose, sucrose, D-arabitol, mucate and 1-O-methyl-alpha-galactopyranoside and their negative reactions for D-sorbitol utilization and the Voges-Proskauer test. On the basis of the phylogenetic analyses, DNA-DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the isolates represent a novel species of the genus Enterobacter, Enterobacter pulveris sp. nov. The type strain is 601/05(T) (=LMG 24057(T)=DSM 19144(T)).
authors.affiliation: Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, 8057 Zurich, Switzerland. stephanr@fsafety.uzh.ch
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
authors.forename: Stefanie
authors.initials: S
authors.lastname: Van Trappen
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.forename: Carol
authors.initials: C
authors.lastname: Iversen
authors.forename: Han
authors.initials: H
authors.lastname: Joosten
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
authors.forename: Angelika
authors.initials: A
authors.lastname: Lehner
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004754:Enterobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004783:Environmental Microbiology; D019649:Food Industry; D005638:Fruit / Q000382:microbiology*; D020459:Genes, rRNA; D007225:Infant Food / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit