Effect of low-dose electron radiation on rat skin wound healing.
The aim of this study was to assess the effect of low-dose electron irradiation on morphological features of the wound healing process in rat skin. Surgical wounds were inflicted with a 2.3 x 1.4 cm template on 84 male rats that were assigned to 4 groups: IG1, immediately irradiated; IG2, irradiated 3 days after inflicting the wound; CG1 and CG2, control groups. Rats in IG1 and IG2 groups had their wounds exposed to 1 Gy of 6 MeV electron beam radiation, immediately after surgery and on the third postoperative day, respectively. Qualitative and histophotometric evaluations of tissue repair structures were carried out. Data were analyzed by ANOVA and Tukey's test (alpha = 0.05) and regression analysis. The repair process was delayed since the first sacrifice time in both irradiated groups, but in IG1, wound healing was closer to that of CG1; whereas in IG2, the delay was more pronounced. Based on the histological findings, it is possible to conclude that a low-dose of electron radiation delayed tissue repair in rat skin. The delay was longer in the skin irradiated 3 days after the beginning of tissue repair. However, the low-energy electron irradiation did not prevent wound healing.
de Almeida
Solange Maria
SM
Department of Oral Diagnosis, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
da Cruz
Adriana Dibo
AD
Ferreira
Rívea Inês
RI
Vizioli
Mário Roberto
MR
Bóscolo
Frab Norberto
FN
D000818:Animals; D001610:Beta Particles; D062109:Dermatologic Surgical Procedures; D004307:Dose-Response Relationship, Radiation; D006025:Glycosaminoglycans / Q000378:metabolism / Q000528:radiation effects*; D006097:Granulation Tissue / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*
D006025:Glycosaminoglycans