title: Proteomic analysis of human osteoarthritic chondrocytes reveals protein changes in stress and glycolysis.
abstract: Osteoarthritis (OA) is characterized by cartilage degradation. The chondrocyte is the only cell type present in mature cartilage, and it is important in the control of cartilage integrity. The aim of this study was to analyze, by a proteomic approach, the changes that are characteristic of OA chondrocytes, and to identify new OA-related proteins. Chondrocytes were isolated from the cartilage of ten OA patients undergoing joint replacement and ten donors with no history of joint disease. Whole-cell proteins were resolved by 2-DE and stained with SYPRO Ruby. Protein expression patterns of 2-DE gels from OA and normal chondrocyte proteins were analyzed with PDQuest 7.3.1 software. OA-related proteins were identified by MALDI-TOF or MALDI-TOF/TOF MS. The results were validated for ANXA1, GSTO1, GRP78, and HSP90beta in cells by Western blotting and in tissue cartilage by immunohistochemistry. Results showed an average of 700 protein spots that were present in the 2-DE gels. Compared to normal chondrocytes, 19 protein spots were found to be significantly increased in OA cells (ratio OA:N> or =2.0, p<0.05), whereas nine were decreased in OA chondrocytes (ratio OA:N< or =0.5, p<0.05). Three stress response proteins were increased (HSP90beta, GRP78, and GRP94) and three proteins involved in glycolysis were decreased (enolase, glyceraldehyde 3-phosphate dehydrogenase, and fructose biphosphate aldolase). Functionally, almost all proteins could be classified as proteins involved in cellular metabolism (33%), structure (21%), or protein targeting (21%).
authors.affiliation: Osteoarticular and Aging Research Laboratory, Proteomics Unit, Rheumatology Division, Juan Canalejo Hospital, Xubias, A Coruña, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ruiz-Romero
authors.forename: Vanessa
authors.initials: V
authors.lastname: Carreira
authors.forename: Ignacio
authors.initials: I
authors.lastname: Rego
authors.forename: Silvia
authors.initials: S
authors.lastname: Remeseiro
authors.forename: María J
authors.initials: MJ
authors.lastname: López-Armada
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Blanco
mesh_terms: D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D000091342:Endoplasmic Reticulum Chaperone BiP; D006019:Glycolysis / Q000502:physiology*; D006801:Humans; D009942:Organometallic Compounds / Q000737:chemistry; D020370:Osteoarthritis, Knee / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D009942:Organometallic Compounds; D020543:Proteome; C515601:Sypro Ruby