Free-radical scavenging capacity using the fenton reaction with rhodamine B as the spectrophotometric indicator.
A spectrophotometric method was developed to measure antioxidant free-radical scavenging capacity. Rhodamine B (RhB) was oxidized by hydroxyl radical generated via the Fenton reaction to yield a photoinactive RhB product. RhB absorption at 550 nm was restored when antioxidant agents scavenged hydroxyl radical to protect RhB from oxidation. On the basis of the dose response of antioxidant recovery capacity, a model was developed to calculate the free-radical scavenging ability. This method was sensitive to a wide range of antioxidant activity with ascorbic acid reference set as one; the antioxidant recovery capacity of quercetin was 635 compared to 2 for benzoic acid.
Yu
Fei
F
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Xu
Da
D
Lei
Rong
R
Li
Na
N
Li
Ke'an
K
D016166:Free Radical Scavengers / Q000032:analysis* / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry; D007202:Indicators and Reagents; D007501:Iron / Q000737:chemistry*; D010084:Oxidation-Reduction; D012235:Rhodamines / Q000737:chemistry*; D013053:Spectrophotometry
C045076:Fenton's reagent; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D012235:Rhodamines; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D007501:Iron; C029773:rhodamine B