Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS.
After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200microl plasma), rapid (runtime 3min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0mg/l using MS/MS detection) method.
Attema-de Jonge
Milly E
ME
Department of Pharmacy, Onze Lieve Vrouwe Gasthuis, Oosterpark 9, 1091 AC Amsterdam, The Netherlands. m.e.attema-dejonge@olvg.nl
Bekkers
Judith M
JM
Oudemans-van Straaten
Heleen M
HM
Sparidans
Rolf W
RW
Franssen
Eric J F
EJ
D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014031:Tobramycin / Q000097:blood*
D000900:Anti-Bacterial Agents; D014031:Tobramycin