title: Three-dimensional modelling of interchain sequence similarities and differences in the coiled-coil segments of keratin intermediate filament heterodimers highlight features important in assembly.
abstract: Using structural data derived from crystal fragments of vimentin, three-dimensional models have been constructed for the major coiled-coil segments (1A, 1B and 2B) in epidermal and hair keratin intermediate filament molecules. Similarity and difference distributions arising from the heterodimer nature of the keratin molecules have been calculated, colour-coded for ease of observation and represented as movie clips. This approach has enabled the spatial distributions of the charged and apolar residues to be visualized along the seam between the chains and on the surface of the molecule, thus providing new insights into the features of the IF molecule that are important in assembly. An observation of note is that one face of both segment 1A and segment 1B is predominantly apolar and, furthermore, contains the bulk of the differences in the charged residues that occur between the two chains. The face rotated by 180 degrees contains far fewer apolar residues. This suggests the likely internal face of segments 1A and 1B and, hence, those sequence and spatial features that are important in assembly. In addition, the similarity distributions of the acidic and basic residues display a period of about 19 residues over much of each of the two faces of segment 1B. The two 19-residue periods are out of phase with respect to one another, however, thus leading to the previously recorded 9.51 residue period in the axial distributions of the acidic and the basic residues. The apparent doubling of the period arises because 9.51 residues corresponds to a non-integral number of turns of alpha-helical coiled coil.
authors.affiliation: Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
authors.forename: Thomasin A
authors.initials: TA
authors.lastname: Smith
authors.forename: David A D
authors.initials: DA
authors.lastname: Parry
mesh_terms: D003198:Computer Simulation; D019281:Dimerization; D007382:Intermediate Filaments / Q000737:chemistry*; D007633:Keratins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D007633:Keratins