title: Sizn1 is a novel protein that functions as a transcriptional coactivator of bone morphogenic protein signaling.
abstract: Bone morphogenic proteins (BMPs) play pleotrophic roles in nervous system development, and their signaling is highly regulated at virtually every step in the pathway. We have cloned a novel gene, Sizn1 (Smad-interacting zinc finger protein), which functions as a transcriptional coactivator of BMP signaling. It positively modulates BMP signaling by interacting with Smad family members and associating with CBP in the transcription complex. Sizn1 is expressed in the ventral embryonic forebrain, where, as we will show, it contributes to BMP-dependent, cholinergic-neuron-specific gene expression. These data indicate that Sizn1 is a positive modulator of BMP signaling and provide further insight into how BMP signaling can be modulated in neuronal progenitor subsets to influence cell-type-specific gene expression and development.
authors.affiliation: Children's Hospital of Philadelphia, ARC/516h, 3400 Civic Center Blvd., Philadelphia, PA 19104, USA.
authors.forename: Ginam
authors.initials: G
authors.lastname: Cho
authors.forename: Youngshin
authors.initials: Y
authors.lastname: Lim
authors.forename: Dina
authors.initials: D
authors.lastname: Zand
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Golden
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D047369:Chromatin Immunoprecipitation; D018076:DNA, Complementary; D004622:Embryo, Mammalian; D004926:Escherichia coli / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D015723:Gene Library; D017930:Genes, Reporter; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007211:Indoles / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008156:Luciferases / Q000032:analysis / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D032446:Myoblasts / Q000166:cytology; D009474:Neurons / Q000378:metabolism; D016548:Prosencephalon / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D020665:Septum of Brain / Q000166:cytology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D051898:Smad1 Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013687:Telencephalon / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D016335:Zinc Fingers
chemical_list: D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D007211:Indoles; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C527302:Sizn1 protein, mouse; D051898:Smad1 Protein; C497401:Smad1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors; C007293:DAPI; D008156:Luciferases