title: Functional identification of the stable transfection C5aR cell line Molt-4.
abstract: The complement C5 anaphylatoxin receptor is a member of the seven transmembrane-spanning G protein-coupled receptor superfamily that signals through Galphai and Galpha16. C5aR is mostly expressed on neutrophils, macrophages and endothelial cells. C5a and C5aR interaction plays an important role in numerous biological effects such as in vivo cytokine storm which results in inflammatory damage. Considering the limitation of collection of human peripheral blood neutrophils and their short half life, the stably transfected cell line for studying the biological effects of C5aR is needed. In this study, we transfected C5aR gene into Molt-4 cell line and examined the function of ectopic C5aR. Our results showed stable expression of the C5aR in Molt-4 cell line and their interaction with human C5a induced ERK1/2 phosphorylation, Ca++ influx. This stable transfected cell line may provide a useful tool for studying signal pathways related to C5a and C5aR interplay and antibody development specific for C5aR.
authors.affiliation: Institute of Basic Medical Sciences, Beijing, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Ruonan
authors.initials: R
authors.lastname: Xu
authors.forename: Jianan
authors.initials: J
authors.lastname: Wang
authors.forename: Gencheng
authors.initials: G
authors.lastname: Han
authors.forename: Guojiang
authors.initials: G
authors.lastname: Chen
authors.forename: Renxi
authors.initials: R
authors.lastname: Wang
authors.forename: Huawei
authors.initials: H
authors.lastname: Wei
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.forename: Yuanfang
authors.initials: Y
authors.lastname: Ma
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D015936:Complement C5a / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D005822:Genetic Vectors; D006801:Humans; D044087:Receptor, Anaphylatoxin C5a / Q000819:agonists / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D014162:Transfection; D019076:Transgenes
chemical_list: D044087:Receptor, Anaphylatoxin C5a; D011994:Recombinant Proteins; D015936:Complement C5a