GATA-6 is a novel transcriptional repressor of the human Tenascin-C gene expression in fibroblasts.
In this study we show that GATA-6 is a novel repressor of TN-C gene expression. We demonstrated that overexpression of GATA-6 in fibroblasts inhibited basal levels, as well as markedly decreased IL-4- and TGF-beta-induced TN-C mRNA and protein levels. A GATA-6 response element was mapped to position -467 to -460 of the TN-C promoter. In addition, we showed that GATA-6 binds this site both in vitro and in vivo.
Ghatnekar
Angela
A
Division of Rheumatology and Immunology, Medical University of South Carolina, CSB 912, SC 29425-2229, USA.
Trojanowska
Maria
M
D001483:Base Sequence; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism; D005347:Fibroblasts; D050993:GATA6 Transcription Factor / Q000235:genetics*; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D008969:Molecular Sequence Data; D019063:Tenascin / Q000235:genetics*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D004268:DNA-Binding Proteins; D050993:GATA6 Transcription Factor; D019063:Tenascin; D016212:Transforming Growth Factor beta; D015847:Interleukin-4