title: Chitosan inserts for periodontitis: influence of drug loading, plasticizer and crosslinking on in vitro metronidazole release.
abstract: Chitosan based metronidazole (MZ) inserts were fabricated by the casting method and characterized with respect to mass and thickness uniformity, metronidazole loading and in vitro metronidazole release kinetics. The fabricated inserts exhibited satisfactory physical characteristics. The mass of inserts was in the range of 5.63 +/- 0.42 to 6.04 +/- 0.89 mg. The thickness ranged from 0.46 +/- 0.06 to 0.49 +/- 0.08 mm. Metronidazole loading was in the range of 0.98 +/- 0.09 to 1.07 +/- 0.07 mg except for batch CM3 with MZ loading of 2.01 +/- 0.08 mg. The inserts exhibited an initial burst release at the end of 24 h, irrespective of the drug to polymer ratio, plasticizer content or cross-linking. However, further drug release was sustained over the next 6 days. Cross-linking with 10% (m/m) of glutaraldehyde inhibited the burst release by approximately 30% and increased the mean dissolution time (MDT) from 0.67 to 8.59 days. The decrease in drug release was a result of reduced permeability of chitosan due to cross-linking.
authors.affiliation: Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi-221005, India.
authors.forename: Romi
authors.initials: R
authors.lastname: Barat
authors.forename: Anegundha
authors.initials: A
authors.lastname: Srinatha
authors.forename: Jayanta K
authors.initials: JK
authors.lastname: Pandit
authors.forename: Shampa
authors.initials: S
authors.lastname: Anupurba
authors.forename: Neelam
authors.initials: N
authors.lastname: Mittal
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000627:therapeutic use; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004337:Drug Carriers; D004339:Drug Compounding; D004343:Drug Implants; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008795:Metronidazole / Q000737:chemistry* / Q000627:therapeutic use; D010518:Periodontitis / Q000188:drug therapy*; D010968:Plasticizers / Q000737:chemistry*; D019946:Propylene Glycol / Q000737:chemistry; D012995:Solubility
chemical_list: D000890:Anti-Infective Agents; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D004343:Drug Implants; D010968:Plasticizers; D008795:Metronidazole; D019946:Propylene Glycol; D048271:Chitosan; D005976:Glutaral