[Effect of Epstein-Barr virus reactivation on gene expression profile of nasopharyngeal carcinoma].
OBJECTIVE
Epstein-Barr virus (EBV) infection plays a key role in the pathogenesis of nasopharyngeal carcinoma (NPC). This study was to explore the effects of the recurrent infection by EBV reactivation on the genomic expression profile of NPC.


METHODS
The microarray expression data from different cell lines subjected to primary infection of EBV+ vs. EBV- targets in NPC and recurrent EBV reactivation were collected from public data depository. Cross comparison, t-test analysis as well as filtering by flag, expression level and fold change were used to analyze the data and identify differential genes. Moreover, a set of web-based applications, such as DAVID (database for annotation, visualization and integrated discovery), pSTIING (protein, signaling, transcriptional interactions and inflammation networks gateway), GATHER (gene annotation tool to help explain relationships) and TELiS (transcription element listening system), were used to analyze and predict the probable expression profile of the differential genes.


RESULTS
As compared with the genes expressed during primary infection of EBV, 25 genes, including DUSP1, TOP1, HOXA9, DEK, PABPC1 and IMPDH2, were differentially expressed during EBV reactivation. Many of them were oncogenic. The differential genes together with related transcriptional factors were interacted mainly through 2 mechanisms: one mainly included TOP1, DUSP1, DUSP6, and RPS28; the other one was a circuit of PITX1, CD9, HOXA9 and IMPDH2.


CONCLUSIONS
The differential genes might participate in EBV reactivation by changing their expression level through two mechanisms, which contributes to the final development of NPC.
Chen
Xia
X
Institute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong, PR China.
Ma
Wen-Li
WL
Liang
Shuang
S
Liao
Zhi-Jun
ZJ
Shang
Tao
T
Zheng
Wen-Ling
WL
D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000472:pathogenicity; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods