Fungal biofilm inhibition by a component naturally produced by Candida albicans yeasts growing as a biofilm.
The purpose of this work was to demonstrate production by the Candida yeasts growing as a biofilm of a compound that reduces candidal biofilm growth. An in vitro model of Candida albicans biofilm associated with silicone catheters was used. Supernatant medium recovered from 24-h-old C. albicans (ATCC 3153) biofilm (S24h) was added during the course of new biofilm formation by four C. albicans strains (182, 444, 2091 or ATCC 3153). Addition of S24h to adherent C. albicans cells induced an inhibition of biofilm growth (P<0.001) but displayed no activity on established biofilms. Ultrafiltration and purification assays demonstrated that the antagonistic molecule is hydrophilic and <3000Da in size.
Cateau
Estelle
E
Equipe de Parasitologie et Mycologie Médicales, LCEE, Université de Poitiers, UMR 6008, 6 Rue de la Milètrie, BP 199, 86034 Poitiers cedex et CHU de Poitiers, France. estellecateau@msn.com
Berjeaud
Jean-Marc
JM
Rodier
Marie-Hélène
MH
Imbert
Christine
C
D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000737:chemistry* / Q000472:pathogenicity; D002404:Catheterization / Q000009:adverse effects / Q000295:instrumentation*; D008826:Microbial Sensitivity Tests; D012825:Silicon / Q000378:metabolism
D012825:Silicon