title: In vivo imaging of elastic fibers using sulforhodamine B.
abstract: Until now, the imaging of elastic fibers was restricted to tissue sections using the endofluorescence properties of elastin or histological dyes. Methods to study their morphology in vivo and in situ have been lacking. We present and characterize a new application of a fluorescent dye for two-photon microscopy: sulforhodamine B (SRB), which is shown to specifically stain elastic fibers in vivo. SRB staining of elastic fibers is demonstrated to be better than using elastin endofluorescence for two-photon microscopy. Our imaging method of elastic fibers is shown to be suitable for simultaneous imaging with both other fluorescent intravital dyes and second-harmonic generation (SHG). We illustrate these findings with intravital imaging of elastic and collagen fibers in muscle epimysium and endomysium and in blood vessel walls. We expect SRB staining to become a key method to study elastic fibers in vivo.
authors.affiliation: INSERM, U836, Grenoble Institut des Neurosciences, Grenoble, F-38042 France. Clement.Ricard@ujf-grenoble.fr
authors.forename: Clément
authors.initials: C
authors.lastname: Ricard
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Vial
authors.forename: Julien
authors.initials: J
authors.lastname: Douady
authors.forename: Boudewijn
authors.initials: B
authors.lastname: van der Sanden
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004547:Elastic Tissue / Q000033:anatomy & histology* / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D051379:Mice; D008819:Mice, Nude; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000098:blood supply / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C022027:lissamine rhodamine B; D003094:Collagen; D004549:Elastin