Structural characterization and DPPH radical scavenging activity of an arabinoglucogalactan from Panax notoginseng root.
The structure of an antiradical arabinoglucogalactan ( 1) from Panax notoginseng roots was determined. This polymeric carbohydrate was obtained through successive phosphate buffer (pH 7.0) extraction after cold-water pretreatment and purification by ion-exchange and gel-filtration chromatography. Monosaccharide analysis, permethylation analysis, NaIO 4 and CrO 3 oxidations, Smith degradation, graded acid hydrolysis, and IR and NMR experiments indicated that 1 possesses a backbone of (1-->3)-linked beta- d-galactofuranosyl residues, with branches of alpha- l-Ara f-(1-->4)-beta- d-Glc p-(1--> residues at O-6. Additionally, 1 exhibited high scavenging activity against DPPH free radicals with a 50% scavenging concentration (SC50) of 11.72 +/- 0.91 microg/mL, suggesting that this arabinoglucogalactan is a potential antiradical.
Wu
Yalin
Y
Department of Chemistry, Columbia University, New York, New York 10027, USA. yw2248@columbia.edu
Wang
Denong
D
D001713:Biphenyl Compounds; D002240:Carbohydrate Sequence; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D053723:Panax notoginseng / Q000737:chemistry*; D010851:Picrates / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013237:Stereoisomerism
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D005685:Galactans; D010851:Picrates; C528200:arabinoglucogalactan; C004931:1,1-diphenyl-2-picrylhydrazyl