Activity of adenosine deaminase and adenylate deaminase on adenosine and 2', 3(')-isopropylidene adenosine: role of the protecting group at different pH values.
The deamination rate of 2',3'-isopropylidene adenosine catalyzed by adenosine deaminase (ADA) from calf intestine and adenylate deaminase (AMPDA) from Aspergillus species has been evaluated and compared with that of the enzymatic reactions of adenosine, to elucidate the influence of the protecting group on enzyme activity.
Alessandrini
Laura
L
Dipartimento di Scienze Precliniche LITA Vialba, Università degli Studi di Milano, Milano, Italy.
Ciuffreda
Pierangela
P
Pavlovic
Radmila
R
Santaniello
Enzo
E
D000659:AMP Deaminase / Q000737:chemistry / Q000378:metabolism*; D000241:Adenosine / Q000737:chemistry*; D000243:Adenosine Deaminase / Q000737:chemistry / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology; D002384:Catalysis; D003641:Deamination; D006863:Hydrogen-Ion Concentration
D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000241:Adenosine