title: Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue.
abstract: 2-Acetyl-6-(dimethylamino)naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 microm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.
authors.affiliation: Molecular Opto-Electronics Laboratory, Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea.
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Kim
authors.forename: Myoung Jin
authors.initials: MJ
authors.lastname: An
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Kyoung J
authors.initials: KJ
authors.lastname: Lee
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry / Q000493:pharmacokinetics* / Q000528:radiation effects; D020013:Calcium Signaling / Q000502:physiology*; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics* / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007031:Hypothalamus / Q000166:cytology / Q000502:physiology; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods; D015394:Molecular Structure; D017785:Photons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium