title: Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.
abstract: Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of selenocysteine into the nascent polypeptide chain. The synthesis of three different selenoproteins from the gram-positive anaerobe Eubacterium acidaminophilum in E. coli was studied. Incorporation of (75)Se into glycine reductase protein B (GrdB1), the peroxiredoxin PrxU, and selenophosphate synthetase (SelD1) was negligible in an E. coli wild-type strain and was fully absent in an E. coli SelB mutant. Selenoprotein synthesis, however, was strongly increased if selB and selC (tRNA(Sec)) from E. acidaminophilum were coexpressed. Putative secondary structures downstream of the UGA codons did not show any sequence similarity to each other or to the E. coli SECIS element. However, mutations in these structures strongly reduced the amount of (75)Se-labeled protein, indicating that they indeed act as SECIS elements. UGA readthrough mediated by the three different SECIS elements was further analyzed using gst-lacZ translational fusions. In the presence of selB and selC from E. acidaminophilum, UGA readthrough was 36 to 64% compared to the respective cysteine-encoding UGC variant. UGA readthrough of SECIS elements present in Desulfomicrobium baculatum (hydV), Treponema denticola (selD), and Campylobacter jejuni (selW-like gene) was also considerably enhanced in the presence of E. acidaminophilum selB and selC. This indicates recognition of these SECIS elements and might open new perspectives for heterologous selenoprotein synthesis in E. coli.
authors.affiliation: Institut für Biochemie und Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes-Strasse 3, 06120 Halle, Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Gursinsky
authors.forename: Daniel
authors.initials: D
authors.lastname: Gröbe
authors.forename: Angelika
authors.initials: A
authors.lastname: Schierhorn
authors.forename: Jana
authors.initials: J
authors.lastname: Jäger
authors.forename: Jan R
authors.initials: JR
authors.lastname: Andreesen
authors.forename: Brigitte
authors.initials: B
authors.lastname: Söhling
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D020029:Base Pairing; D001483:Base Sequence; D015153:Blotting, Western; D018388:Codon, Terminator / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005051:Eubacterium / Q000235:genetics*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism; D017279:Selenocysteine / Q000235:genetics / Q000378:metabolism*; D051140:Selenoproteins / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D018388:Codon, Terminator; D012333:RNA, Messenger; D012345:RNA, Transfer, Amino Acid-Specific; C061878:SelB protein, Bacteria; D051140:Selenoproteins; C036928:tRNA, selenocysteine-; D017279:Selenocysteine