Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen.
The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.
Tillmann
Ramona Liza
RL
University of Bonn, Institute for Virology, Bonn, Germany.
Simon
Arne
A
Müller
Andreas
A
Schildgen
Oliver
O
D001483:Base Sequence; D002648:Child; D015331:Cohort Studies; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018357:Respiratory Syncytial Virus Infections / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D017931:DNA Primers