[Simultaneous determination of seven sulfonamides and metronidazole and chloramphenicol in cosmetics by high performance liquid chromatography].
A method for the simultaneous determination of seven sulfonamides (sulfacetamide, sulfapyridine, sulfamerazine, sulfamethazine, sulfamater, sulfamonomethoxine, sulfamethoxazole) and metronidazole, chloramphenicol in cosmetics has been developed by high performance liquid chromatography with a photodiode array detector (HPLC-PDA). The chromatographic column Atlantis dC18 (150 mm x 4.6 mm, 5 microm) was used with acetonitrile-0.1% formic acid (2:8, v/v) aqueous solution as mobile phase at a flow rate of 1.0 mL/min. Samples were extracted by mobile phase and detected by HPLC-PDA at 268 nm. The limits of quantification were 3 - 80 microg/g. The correlation coefficients of linear calibration curves were over 0. 999 3 within the sulfonamide concentration range of 20 - 200 microg/mL and metronidazole and chloramphenicol concentration range of 40 - 400 microg/mL. Average recoveries were 83.8% - 105.3% at the spiked levels of 50 microg/mL and 150 microg/mL for sulfonamides, and 100 microg/mL and 300 microg/mL for metronidazole and chloramphenicol. The relative standard deviations were below 5%. This method can be routinely used for the determination of seven sulfonamides and metronidazole, chloramphenicol in cosmetics.
Wang
Ping
P
Beijing Center for Disease Control and Prevention, Beijing 100013, China. ping_wang@tom.com
Li
Jie
J
Zheng
Hehui
H
D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D008795:Metronidazole / Q000032:analysis*; D015203:Reproducibility of Results; D013449:Sulfonamides / Q000032:analysis*
D003358:Cosmetics; D013449:Sulfonamides; D008795:Metronidazole; D002701:Chloramphenicol