Biomechanical properties of decellularized porcine pulmonary valve conduits.
Tissue-engineered heart valves constructed from a xenogeneic or allogeneic decellularized matrix might overcome the disadvantages of current heart valve substitutes. One major necessity besides effective decellularization is to preserve the biomechanical properties of the valve. Native and decellularized porcine pulmonary heart valve conduits (PPVCs) (with [n = 10] or without [n = 10] cryopreservation) were compared to cryopreserved human pulmonary valve conduits (n = 7). Samples of the conduit were measured for wall thickness and underwent tensile tests. Elongation measurement was performed with a video extensometer. Decellularized PPVC showed a higher failure force both in longitudinal (+73%; P < 0.01) and transverse (+66%; P < 0.001) direction compared to human homografts. Failure force of the tissue after cryopreservation was still higher in the porcine group (longitudinal: +106%, P < 0.01; transverse: +58%, P < 0.001). In comparison to human homografts, both decellularized and decellularized cryopreserved porcine conduits showed a higher extensibility in longitudinal (decellularized: +61%, P < 0.001; decellularized + cryopreserved: +51%, P < 0.01) and transverse (decellularized: +126%, P < 0.001; decellularized + cryopreserved: +118%, P < 0.001) direction. Again, cryopreservation did not influence the biomechanical properties of the decellularized porcine matrix.
Seebacher
Gernot
G
Department of Cardiothoracic Surgery, Medical University of Vienna, Vienna, Austria.
Grasl
Christian
C
Stoiber
Martin
M
Rieder
Erwin
E
Kasimir
Marie-Theres
MT
Dunkler
Daniela
D
Simon
Paul
P
Weigel
Günter
G
Schima
Heinrich
H
D000818:Animals; D001696:Biomechanical Phenomena; D015925:Cryopreservation; D005615:Freezing; D006801:Humans; D066298:In Vitro Techniques; D011664:Pulmonary Valve / Q000502:physiology* / Q000637:transplantation; D013552:Swine; D019737:Transplants