[A new bromine-containing reagent for cysteine modification].
5-Bromo-2[(2-iodoacetyl)amino]benzenesulfonic acid (AIBSA), a reagent for modification of free of cysteine thiol groups in proteins and peptides, was synthesized. Rate constants of its interaction with thiol groups were determined. The presence of a bromine atom allows an easy identification of the AIBSA-labeled peptides in mass spectra due to the characteristic isotope distribution. The compound is stable in solution and under exposure to light.
Minaeva
T V
TV
Danilovtseva
E N
EN
Annenkov
V V
VV
Novikov
A V
AV
Vereshchagin
A L
AL
Grachev
M A
MA
D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects; D001966:Bromine / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D007460:Iodoacetamide / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000528:radiation effects; D011506:Proteins / Q000737:chemistry*
C526703:5-bromo-2-((2-iodoacetyl)amino)benzenesulfonic acid; D001557:Benzenesulfonates; D011506:Proteins; D003545:Cysteine; D001966:Bromine; D007460:Iodoacetamide