title: Biotransformation of Panax notoginseng saponins into ginsenoside compound K production by Paecilomyces bainier sp. 229.
abstract: OBJECTIVE
  Development and optimization of an efficient and inexpensive biotransformation process for ginsenoside compound K production by Paecilomyces bainier sp. 229.
  
  
  RESULTS
  We have determined the optimum culture conditions required for the efficient production of ginsenoside compound K by P. bainier sp. 229 via biotransformation of ginseng saponin substrate. The optimal medium constituents were determined to be: 30 g sucrose, 30 g soybean steep powder, 1 g wheat bran powder, 1 g (NH(4))(2)SO(4), 2 g MgSO(4) x 7H(2)O and 1 g CaCl(2) in 1 l of distilled water. An inoculum size of 5-7.5% with an optimal pH range of 4.5-5.5 was essential for high yield.
  
  
  CONCLUSIONS
  The Mol conversion quotient of ginseng saponins increased from 21.2% to 72.7% by optimization of the cultural conditions. Scale-up in a 10 l fermentor, under conditions of controlled pH and continuous air supply in the optimal medium, resulted in an 82.6% yield of ginsenoside compound K.
  
  
  CONCLUSIONS
  This is the first report on the optimization of culture conditions for the production of ginsenoside compound K by fungal biotransformation. The degree of conversion is significantly higher than previous reports. Our method describes an inexpensive, rapid and efficient biotransformation system for the production of ginsenoside compound K.
authors.affiliation: Department of Biosynthetic Drug, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Yan
authors.forename: J-Y
authors.initials: JY
authors.lastname: Li
authors.forename: X-C
authors.initials: XC
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Zhou
mesh_terms: D019149:Bioreactors / Q000382:microbiology; D001711:Biotransformation; D003470:Culture Media; D005285:Fermentation; D036145:Ginsenosides / Q000032:analysis / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D009172:Mycology / Q000379:methods; D010143:Paecilomyces / Q000378:metabolism*; D053723:Panax notoginseng / Q000378:metabolism*; D012503:Saponins / Q000378:metabolism*; D013025:Glycine max; D013395:Sucrose
chemical_list: D003470:Culture Media; D036145:Ginsenosides; D012503:Saponins; D013395:Sucrose; C112772:ginsenoside compound K