title: [Fingerprinting of Fuzhisan by high-performance liquid chromatography with ultraviolet and evaporative light scattering detector].
abstract: OBJECTIVE
  To establish a method for fingerprinting of Fuzhisan (FZS, a traditional Chinese medicinal preparation) using high-performance liquid chromatography with ultraviolet and evaporative light scattering detector (HPLC-UV/ELSD) to allow simultaneous determination of 5 major constituents in the preparation.
  
  
  METHODS
  HPLC-UV/ELSD analysis was performed on water AlltechC18 column (5 microm, 4.6 mm x 250 mm) with a mixture of acetonitrile (A) and 0.1% acetice acid water (B) as the mobile phase. The solvent A gradient for elution was 0, 12%; 25, 20%; 30, 20%; 75, 30%; 105, 40%; 120, 80%; 130, 12%, with the flow rate of 1.0 ml/min; and the column temperature at 30 degrees . The detective wavelength was 335 nm, drift tube temperature was 80 degrees , pressure of nebulizer gas was 25 psi. The similarities between the HPLC-UV/ELSD fingerprints of the 12 extracts were calculated using similarity evaluation software.
  
  
  RESULTS
  The fingerprint of FZS was established and the 5 major constituents were identified. The complementarity between the fingerprints of UV and ELSD was analyzed, showing good correlation between 12 batches of FZS.
  
  
  CONCLUSIONS
  The method for fingerprinting can simultaneously characterize the main chemical constituents in FZS and allows stable, effective and comprehensive quality control and evaluation of FZS for a single sample.
authors.affiliation: Department of Neurology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. zjk7486@yahoo.com.cn
authors.forename: Jing-kun
authors.initials: JK
authors.lastname: Zhao
authors.forename: De-sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Shu-rong
authors.initials: SR
authors.lastname: Duan
authors.forename: Jian-xiu
authors.initials: JX
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.forename: Wen-lan
authors.initials: WL
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D008027:Light; D011786:Quality Control; D012542:Scattering, Radiation; D014466:Ultraviolet Rays
chemical_list: D004365:Drugs, Chinese Herbal; C543479:fuzhisan