Comparative proteomics analysis of light responses in cryptochrome1-304 and Columbia wild-type 4 of Arabidopsis thaliana.
The blue light photoreceptor mutant cryptochrome1-304 (cry1-304) and Columbia wild-type 4 (col-4) of Arabidopsis thaliana were grown under white light and blue light, and in the dark. To study the difference in protein expression levels between cry1-304 and col-4, a proteomic approach was applied based on 2-D gel electrophoresis. Twenty-one different protein spots were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of four genes corresponding to four protein spots was analyzed by semiquantitative reverse transcription-polymerase chain reaction. We applied analytical procedures to study cry1-304 and col-4, and found that the differentially expressed proteins formed six clusters reflecting co-regulation. This assessment was consistent with the known physiological responses of plants to light.
Yang
Yuejun
Y
College of Life Science and Biotechnology, Hunan University, Changsha 410082, China.
Li
Yan
Y
Li
Xu
X
Guo
Xinhong
X
Xiao
Xiaojuan
X
Tang
Dongying
D
Liu
Xuanming
X
D017360:Arabidopsis / Q000502:physiology* / Q000528:radiation effects*; D029681:Arabidopsis Proteins; D056931:Cryptochromes; D005420:Flavoproteins / Q000378:metabolism*; D008027:Light; D005810:Multigene Family / Q000502:physiology*; D018462:Photobiology / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D011829:Radiation Dosage
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D056931:Cryptochromes; D005420:Flavoproteins; D020543:Proteome