[Phylogeny relationship and molecular identification of ten Huperzia species (Huperziaceae) based on matK gene sequences].
OBJECTIVE
To study the phylogeny relationship and molecular identification of 10 species from Huperzia (Huperziaceae) based on matK gene sequences data.


METHODS
Total DNA of nine species from Huperzia was extracted; matK gene sequence was amplified by PCR. PCR product was directly sequenced after purification.


RESULTS
The chloroplast matK gene nucleotide sequences from 9 species of Huperzia species were sequenced. The matK gene nucleotide sequences length was 1 589 bp. Analysis with Huperzia lucidula matK gene nucleotide sequences (download from GenBank) and taking Lycopodiella cernua as outgroup, Maximum Parsimony, Neighbor-Joining analyses and genetic distances were conducted using MEGA 3.1 software. 35 variable sites and 35 parsimony informative sites have been found. Pairwise genetic distances among 10 species of Huperzia was 1.59% - 0.25%.


CONCLUSIONS
The results were consistent with the taxonomy in morphological of Huperzia. But H. longipetiolata and H. serrata were resolved into in different clade. There are 19 different sites of matK gene sequences between H. longipetiolata and H. serrata, the genetic distances is 0.121%. It is suggested H. longipetiolata should be as an independent species.
Ji
Sheng-Guo
SG
School of Pharmacy, Fudan University, Shanghai 200032, China.
Pan
Sheng-Li
SL
Wang
Jun
J
Huo
Ke-Ke
KK
D018742:DNA, Chloroplast / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics*; D032501:Huperzia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal / Q000145:classification / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018742:DNA, Chloroplast; D018744:DNA, Plant; D010940:Plant Proteins; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases