title: Growth inhibition of breast cancer cell line MCF-7 by siRNA silencing of Wilms tumor 1 gene.
abstract: BACKGROUND
  RNA interference (RNAi) is sequence-specific inhibition of gene expression induced by double-stranded RNA.
  
  
  OBJECTIVE
  Define the role of Wilms tumor 1 gene (WT1) in breast cancer oncogenesis using RNAi.
  
  
  METHODS
  MCF-7 breast cancer cells, which express a high level of WT1, were transfected with synthetic small interfering RNA (siRNA) targeting WT1 (siRNA(WT1)) resulting in inhibition of WTI expression, as well as growth, in a dose- and time-dependent manner.
  
  
  RESULTS
  The minimum concentration of siRNA(WT1) for growth inhibition and WT1 silencing was 25 nM and 50 nM respectively. WT1 expression was completely abolished at 200 nM siRNA,.
  
  
  CONCLUSIONS
  These data suggest that WTI1is indispensable for the survival of breast cancer MCF-7 cell line.
authors.affiliation: Biomedical Sciences Department, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
authors.forename: Ruxapon
authors.initials: R
authors.lastname: Navakanit
authors.forename: Potchanapond
authors.initials: P
authors.lastname: Graidist
authors.forename: Wilairat
authors.initials: W
authors.lastname: Leeanansaksiri
authors.forename: Chavaboon
authors.initials: C
authors.lastname: Dechsukum
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering