Generation of a monoclonal antibody specific for tissue-nonspecific alkaline phosphatase and its use in a clinical diagnostic study.
Tissue-nonspecific alkaline phosphatase (TNSALP) in serum comprises liver alkaline phosphatase (liver-ALP) and bone alkaline phosphatase (bone-ALP). Liver-ALP is a marker of liver disease; thus a specific method for its measurement would be useful. Measurement of ALP by electrophoresis is difficult, although all of the isozymes can be assessed simultaneously. Total ALP can also be measured by automated analyzer, but it is difficult to determine the cause of a high ALP value because bone-, intestine-, placenta-, and tumor-ALP are measured together. Thus, anti-TNSALP monoclonal antibodies that can resolve these problems are needed. Here we have generated an anti-TNSALP monoclonal antibody, 3-29-3R. This clone has specificity to liver-ALP rather than to bone-ALP. In electrophoresis, 3-29-3R reacted with TNSALP and shifted the bands. The use of 3-29-3R enabled easy interpretation of the results. Furthermore, we tested 3-29-3R by developing an immunocapture enzymatic assay (IEA). Preliminary results of the IEA show that this method is effective for measurement of liver-ALP. Thus, the monoclonal antibody that we have established may be a useful tool for clinical diagnosis.
Ohashi
Tatsuya
T
Nitto Boseki Corporation Ltd, Biochemical Laboratory, Koriyama, Japan. oohashit@jn.nittobo.co.jp
Sunaga
Masahiko
M
Miura
Toshihide
T
Sasagawa
Kumiko
K
Sato
Yasuhito
Y
Ohashi
Wataru
W
Katagiri
Ken
K
Katano
Yoshinori
Y
Kiyokawa
Iwao
I
Kojima
Ryo
R
Tomonaga
Takeshi
T
Nomura
Fumio
F
Amizuka
Norio
N
Oda
Kimimitsu
K
Sato
Toyoji
T
Katayama
Katsuhiro
K
D000328:Adult; D000469:Alkaline Phosphatase / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000378:metabolism; D001859:Bone Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000276:immunology; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas; D008107:Liver Diseases / Q000175:diagnosis / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009928:Organ Specificity / Q000276:immunology; D013379:Substrate Specificity / Q000276:immunology; D014018:Tissue Distribution / Q000276:immunology
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase