title: Evaluation of the cytocompatibility of mixed bovine bone.
abstract: Treatment of bovine bone with peroxides and chaotropic agents aims to obtain an acellular bone matrix that is able to maintain the collagen-apatite complex and a higher mechanical resistance, a mixed biomaterial hereby named mixed bovine bone (MBB). The purpose of this study was to evaluate the cytocompatibility of MBB and cell-MBB interaction. Cell morphology, number of viable cells, ability to reduce methyltetrazolium and to incorporate neutral red upon exposure to different concentrations of the hydrosoluble extract of MBB were assessed in Balb-c 3T3 cells according to ISO 10993-5 standard. The interaction between cells and MBB surface was evaluated by scanning electron microscopy. The water-soluble MBB extracts were cytotoxic and led to cell death possibly due to its effect on mitochondrial function and membrane permeability. Cells plated directly onto the MBB did not survive, although after dialysis and material conditioning in DMEM + 10% FCS, the cells adhered and proliferated onto the material. It may be concluded that, in vitro, water-soluble MBB extracts were cytotoxic. Nevertheless, MBB cytotoxic effect was reverted by dialysis resulting in a material that is suitable for cell based-therapy in the bioengineering field.
authors.affiliation: Department of Biological Sciences, School of Dentistry of Bauru, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Esther Rieko
authors.initials: ER
authors.lastname: Takamori
authors.forename: Eduardo Aleixo
authors.initials: EA
authors.lastname: Figueira
authors.forename: Rumio
authors.initials: R
authors.lastname: Taga
authors.forename: Mari Cleide
authors.initials: MC
authors.lastname: Sogayar
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix; D018786:Bone Substitutes / Q000633:toxicity*; D002417:Cattle; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D008422:Materials Testing / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D018709:Statistics, Nonparametric; D018675:Toxicity Tests / Q000379:methods; D014183:Transplantation, Heterologous / Q000009:adverse effects*
chemical_list: D018786:Bone Substitutes