[Method of antibody analysis based on visual protein array].
OBJECTIVE
To establish a novel method for antibody analysis based on visual protein microarray.


METHODS
The antibodies spotted on the aldehyde-modified slides were incubated with corresponding antigens labeled by biotin, followed by silver enhancement detection method, and the chromogenic images were scanned by CCD camera.


RESULTS
The affinity and specificity of each antibody was assayed by this method and six antibodies were found to have higher specificity than the others. A detection limit of 0.4 microg/L was obtained for anti-globulinIIIC013, anti-albumin HPSIIB007, and anti-albumin HPSIIIB007.


CONCLUSIONS
This method is simple, fast and visual. In addition, it needs less sample amount than traditional immunoassay and has potential to achieve high throughput.
Yu
Zhe
Z
College of Life Science, Capital Normal University, Beijing 100037, China. yzhsd@163.com
Liu
Zhi-hong
ZH
Wu
Ying-song
YS
Gao
Jian-en
JE
Sun
Qi-hong
QH
Li
Ming
M
Yin
Li-ping
LP
Xu
Dan-ke
DK
D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000941:Antigens; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods; D005456:Fluorescent Dyes; D006967:Hypersensitivity; D007118:Immunoassay / Q000379:methods; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007136:Immunoglobulins / Q000032:analysis*; D057230:Limit of Detection; D053768:Metal Nanoparticles; D040081:Protein Array Analysis; D040901:Proteomics; D012680:Sensitivity and Specificity
D000906:Antibodies; D000941:Antigens; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D007136:Immunoglobulins; D007073:Immunoglobulin E; D001710:Biotin