Time course of changes in in vitro sarcoplasmic reticulum Ca2+-handling and Na+-K+-ATPase activity during repetitive contractions.
Time-dependent changes in sarcoplasmic reticulum (SR) Ca2+-handling and Na+-K+-ATPase activity, as assessed in vitro, were investigated in the superficial (GS) and deep regions (GD) of rat gastrocnemius muscles undergoing short-term (up to 30 min) electrical stimulation. There was a rapid and progressive loss of force output during the first 5 min of stimulation. For GS, significant depressions (P < 0.05) in SR Ca2+-uptake rate and Ca2+-ATPase activity were observed during only the first 1 min. No further reductions occurred with stimulation time. SR Ca2+-release rate was significantly (P < 0.05) decreased at 3 min. For GD, significant reductions (P < 0.05) in Ca2+-uptake rate, Ca2+-release rate and Ca2+-ATPase activity were manifested after 3, 5, and 5 min, respectively. A decay in Na+-K+-ATPase activity was found only in 1-min stimulated GD and 30-min stimulated GS. After 30 min, the depressed functions reverted to resting levels in GD but not in GS. The alterations in any variables examined were not parallel with changes in force output. These results suggest that, at least under the conditions used in this study, in vivo disruptions in cation regulation mediated by vigorous contractile activity would be attributable primarily to events other than structural alterations to the respective proteins.
Mishima
Takaaki
T
Graduate School of Biosphere Science, Hiroshima University, 1-7-1 Kagamiyama, Higashihiroshima-shi, Hiroshima, Japan.
Yamada
Takashi
T
Sakamoto
Makoto
M
Sugiyama
Minako
M
Matsunaga
Satoshi
S
Wada
Masanobu
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004558:Electric Stimulation; D005978:Glutathione / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000201:enzymology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D012508:Sarcolemma / Q000201:enzymology*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013997:Time Factors
D019344:Lactic Acid; D006003:Glycogen; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D002118:Calcium