Prevalence and genetic characterization of canine parvoviruses in Korea.
The prevalence of canine parvovirus (CPV) variants in dog was investigated in a total of 51 fecal samples submitted over a 2-year period (2005-2007) in Korea. The CPV VP2 gene was amplified and sequenced from the fecal samples, and the results indicated that of the 51 samples, 49 samples belong to the CPV-2a family, 1 to CPV-2b, and the remaining 1 to CPV-2a variant. The VP2 gene of 20 isolates was sequenced and phylogenetic analysis was conducted. With one exception, all of the isolates were closely related to a Taiwanese isolate (CPV T37) and they formed geographical patterns of VP2 gene nucleotide sequences. Our finding showed that CPV-2a was the predominant type and CPV-2b and CPV-2a variant also existed in Korea. Using the hemagglutination inhibition (HI) and the neutralization (Nt) test, the animals inoculated with CPV-2 developed low antibody titers against the CPV-2 variants in laboratory animal was also identified.
Kang
Bo-Kyu
BK
Research Unit, Green Cross Veterinary Products, Yongin, Kiheung 227-5, Korea.
Song
Dae-Sub
DS
Lee
Chul-Seung
CS
Jung
Kwon-Il
KI
Park
Seong-Jun
SJ
Kim
Eun-Mi
EM
Park
Bong-Kyun
BK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004285:Dogs; D005243:Feces / Q000821:virology; D007723:Korea / Q000453:epidemiology; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D015995:Prevalence; D016415:Sequence Alignment