title: Optimized liquid chromatography-mass spectrometry approach for the isolation of minor stress biomarkers in plant extracts and their identification by capillary nuclear magnetic resonance.
abstract: A LC-MS approach is presented for the isolation of minor key plant biomarkers, in view of their characterization by NMR at the microgram scale. Due to the complexity of plant extracts, the purification of metabolites present in low concentrations is critical. The strategy used relies on the optimization of the chromatographic analysis using ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS), thanks to modelling software. The optimized method is then transferred to semi-preparative LC conditions with MS detection. The approach is illustrated by the isolation of wound-induced jasmonate derivatives revealed by a metabolomic study in Arabidopsis thaliana leaves and their subsequent characterization by capillary NMR (CapNMR).
authors.affiliation: Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, EPGL, University of Geneva, CH-1211 Geneva 4, Switzerland.
authors.forename: Gaetan
authors.initials: G
authors.lastname: Glauser
authors.forename: Davy
authors.initials: D
authors.lastname: Guillarme
authors.forename: Elia
authors.initials: E
authors.lastname: Grata
authors.forename: Julien
authors.initials: J
authors.lastname: Boccard
authors.forename: Aly
authors.initials: A
authors.lastname: Thiocone
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Carrupt
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Veuthey
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D015415:Biomarkers; D010936:Plant Extracts