title: DNA microarray analysis of central carbohydrate metabolism: glycolytic/gluconeogenic carbon switch in the hyperthermophilic crenarchaeum Thermoproteus tenax.
abstract: In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed.
authors.affiliation: Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany. melanie.zaparty@uni-due.de
authors.forename: Melanie
authors.initials: M
authors.lastname: Zaparty
authors.forename: Alexander
authors.initials: A
authors.lastname: Zaigler
authors.forename: Claudia
authors.initials: C
authors.lastname: Stamme
authors.forename: Jörg
authors.initials: J
authors.lastname: Soppa
authors.forename: Reinhard
authors.initials: R
authors.lastname: Hensel
authors.forename: Bettina
authors.initials: B
authors.lastname: Siebers
mesh_terms: D050260:Carbohydrate Metabolism; D019848:Gene Expression Regulation, Archaeal / Q000187:drug effects; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006019:Glycolysis; D006358:Hot Temperature; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics; D041542:Thermoproteus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D005947:Glucose