Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in Saccharomyces cerevisiae.
The Mv1751 gene product is thought to catalyze the first step in the N-glycosylation pathway in Methanococcus voltae. Here, we show that a conditional lethal mutation in the alg7 gene (N-acetylglucosamine-1-phosphate transferase) in Saccharomyces cerevisiae was successfully complemented with Mv1751, highlighting a rare case of cross-domain complementation.
Shams-Eldin
Hosam
H
Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
Chaban
Bonnie
B
Niehus
Sebastian
S
Schwarz
Ralph T
RT
Jarrell
Ken F
KF
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics*; D005816:Genetic Complementation Test / Q000379:methods; D006031:Glycosylation; D017017:Methanococcus / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D020539:Sequence Analysis, Protein; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics*
D019843:Archaeal Proteins; D029701:Saccharomyces cerevisiae Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C030012:UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase