Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.
Heal
William P
WP
Department of Chemistry, South Kensington Campus, Imperial College, London, UK.
Wickramasinghe
Sasala R
SR
Bowyer
Paul W
PW
Holder
Anthony A
AA
Smith
Deborah F
DF
Leatherbarrow
Robin J
RJ
Tate
Edward W
EW
D000217:Acyltransferases / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D010963:Plasmodium falciparum / Q000201:enzymology; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D000217:Acyltransferases; C050480:glycylpeptide N-tetradecanoyltransferase