Dihydrotanshinone I inhibits angiogenesis both in vitro and in vivo.
Dihydrotanshinone I (DI), a naturally occurring compound extracted from Salvia miltiorrhiza Bunge, has been reported to have cytotoxicity to a variety of tumor cells. In this study, we investigated its anti-angiogenic capacity in human umbilical vein endothelial cells. DI induced a potent cytotoxicity to human umbilical vein endothelial cells, with an IC(50) value of approximately 1.28 microg/ml. At 0.25-1 microg/ml, DI dose-dependently suppressed human umbilical vein endothelial cell migration, invasion, and tube formation detected by wound healing, Transwell invasion and Matrigel tube formation assays, respectively. Moreover, DI showed significant in vivo anti-angiogenic activity in chick embryo chorioallantoic membrane assay. DI induced a 61.1% inhibitory rate of microvessel density at 0.2 microg/egg. Taken together, our results showed that DI could inhibit angiogenesis through suppressing endothelial cell proliferation, migration, invasion and tube formation, indicating that DI has a potential to be developed as a novel anti-angiogenic agent.
Bian
Weipeng
W
School of Life Science, East China Normal University, Shanghai 200062, China.
Chen
Fei
F
Bai
Ling
L
Zhang
Ping
P
Qin
Wenxin
W
D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D005663:Furans; D006801:Humans; D020128:Inhibitory Concentration 50; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology*; D010616:Phenanthrenes / Q000494:pharmacology*; D011809:Quinones; D014471:Umbilical Veins / Q000166:cytology
D020533:Angiogenesis Inhibitors; D005663:Furans; D010616:Phenanthrenes; D011809:Quinones; C000713095:dihydrotanshinone I