title: Effective gene transfer to melanoma cells using bacterial ghosts.
abstract: Bacterial ghosts (BG) are cell envelopes preparations of Gram-negative bacteria devoid of cytoplasmic content produced by controlled expression of PhiX174 plasmid-encoded lysis gene E. Eight melanoma cell lines were investigated for their capacity to bind and phagocyte BG derived from Escherichia coli NM522 and Mannheimia haemolytica A23. High capability to bind BG was observed in almost all of the analyzed cell lines, furthermore cells were able to take up BG independently of the used bacterial species. Further, transfection efficiency of BG loaded with DNA in vitro was measured. The Bowes cells exhibited a high expression level of GFP and the incubation of cells with plasmid loaded BG led up to 82% transfection efficiency.
authors.affiliation: Cancer Research Institute, Slovak Academy of Sciences, Vlarska, Bratislava, Slovakia. pavol.kudela@savba.sk
authors.forename: Pavol
authors.initials: P
authors.lastname: Kudela
authors.forename: Susanne
authors.initials: S
authors.lastname: Paukner
authors.forename: Ulrike Beate
authors.initials: UB
authors.lastname: Mayr
authors.forename: Dana
authors.initials: D
authors.lastname: Cholujova
authors.forename: Gudrun
authors.initials: G
authors.lastname: Kohl
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Schwarczova
authors.forename: Jozef
authors.initials: J
authors.lastname: Bizik
authors.forename: Jan
authors.initials: J
authors.lastname: Sedlak
authors.forename: Werner
authors.initials: W
authors.lastname: Lubitz
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D016978:Mannheimia haemolytica / Q000235:genetics*; D008545:Melanoma / Q000235:genetics*; D010587:Phagocytosis; D010957:Plasmids; D012878:Skin Neoplasms / Q000235:genetics*; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins