The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response.
CD99, a glycoprotein found on the surfaces of leukocytes and concentrated at the borders of endothelial cells, plays a major role in the migration of leukocytes across endothelial cells into sites of inflammation, and has other roles in thymocyte development. The human and mouse genomes encode only two proteins related to CD99. One of these, XGA, is a red blood cell surface antigen. The function of the other, CD99-like 2 (CD99L2), is not known. We cloned mouse CD99L2 and used CD99L2 isolated from transfected cells to raise specific antibodies. Similar to human CD99, CD99L2 was expressed at the borders between transfected cells as well as on mouse leukocytes and vascular endothelial cells in situ. Transfection of L cell fibroblasts with CD99L2 imparted to them the ability to adhere to each other in a divalent cation-dependent, homophilic manner. Anti-CD99L2 antibody blocked influx of neutrophils and monocytes into a site of inflammation in vivo.
Schenkel
Alan R
AR
Weill Cornell Medical College, Department of Pathology and Laboratory Medicine, New York, NY, USA.
Dufour
Eric M
EM
Chew
Tina W
TW
Sorg
Emily
E
Muller
William A
WA
D000071443:12E7 Antigen; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism*; D019556:COS Cells; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D002522:Chlorocebus aethiops; D042783:Endothelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000071443:12E7 Antigen; D000906:Antibodies; D015703:Antigens, CD; D000954:Antigens, Surface; C522605:Cd99 protein, mouse; D015815:Cell Adhesion Molecules