title: Fungal communities in decaying sapwood and heartwood of a conifer Keteleeria evelyniana.
abstract: Fungal communities in decaying sapwood and heartwood of K. evelyniana were demonstrated through construction of four 18 S rRNA gene libraries. The 210 sequenced clones were clustered into 11 subgroups, belonging to Basidiomycota (71.9%) and to Ascomycota (22.4%) and unclassified (1 subgroup; 5.7%). The heartwood displayed higher species richness than the sapwood. Basidiomycota were dominant in either the heartwood or the sapwood. Phylogenetically diverse Homobasidiomycetes were detected in the heartwood, contrary to the sapwood, where Heterobasidiomycetes were detected. Clones close to Spongipellis unicolor dominated in the heartwood (21 of 99 clones), while those close to Hydnochaete olivacea dominated in the sapwood (41 of 111 clones). The common species between the two parts were those related to S. unicolor, Calocera cornea, Debaryomyces hansenii, Davidiella tassiana, and Nomuraea rileyi and those from Chaetothyriomycetes.
authors.affiliation: Yunnan University, Kunming, 650091, People's Republic of China. zhhb@ynu.edu.cn
authors.forename: Han-Bo
authors.initials: HB
authors.lastname: Zhang
authors.forename: Ming-Xia
authors.initials: MX
authors.lastname: Yang
authors.forename: Ran
authors.initials: R
authors.lastname: Tu
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhao
mesh_terms: D044822:Biodiversity; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005658:Fungi / Q000145:classification* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D028201:Pinaceae / Q000382:microbiology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014934:Wood / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S