title: Human bocavirus (HBoV) in Thailand: clinical manifestations in a hospitalized pediatric patient and molecular virus characterization.
abstract: OBJECTIVE
  Human bocavirus (HBoV), a novel virus, which based on molecular analysis has been associated with respiratory tract diseases in infants and children have recently been studied worldwide. To determine prevalence, clinical features and perform phylogenetic analysis in HBoV infected Thai pediatric patients.
  
  
  METHODS
  HBoV was detected from 302 nasopharyngeal (NP) suctions of pediatric patients with acute lower respiratory tract illness and sequenced applying molecular techniques.
  
  
  RESULTS
  The incidence of HBoV infection in pediatric patients amounted to 6.62% with 40% co-infected with other respiratory viruses. There were no clinical specific manifestations for HBoV; however, fever and productive cough were commonly found. Generalized rales and wheezing were detected in most of the patients as well as perihilar infiltrates. The alignment and phylogenetic analysis of partial VP1 genes showed minor variations.
  
  
  CONCLUSIONS
  Our results indicated that HBoV can be detected in nasopharyngeal aspirate specimens from infants and children with acute lower respiratory tract illness.
authors.affiliation: Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Chieochansin
authors.forename: Rujipat
authors.initials: R
authors.lastname: Samransamruajkit
authors.forename: Salin
authors.initials: S
authors.lastname: Chutinimitkul
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Thitikul
authors.initials: T
authors.lastname: Hiranras
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000293:Adolescent; D052660:Bocavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D006760:Hospitalization; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D048348:Reverse Transcription; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology
chemical_list: D036022:Capsid Proteins