title: Effects of introducing negative charges into the molecular surface of thermolysin by site-directed mutagenesis on its activity and stability.
abstract: Thermolysin is remarkably activated and stabilized by neutral salts, and surface charges are suggested important in its activity and stability. The effects of introducing negative charge into the molecular surface on its activity and stability are described. Seven serine residues were selected, and each of them was changed for aspartate by site-directed mutagenesis in a thermolysin mutant. In the hydrolysis of N-[3-(2-furyl)acryloyl]-glycyl-l-leucine amide, the k(cat)/K(m) values of all mutants were almost similar to that of the wild-type enzyme (WT). However, those of six out of seven mutants were enhanced 17-19 times with 4 M NaCl, being slightly higher than WT. The remaining casein-hydrolyzing activities of the S53D and S65D mutants (Ser53 and Ser65 are replaced with Asp, respectively) after 30-min incubation with 10 mM CaCl(2) at 85 degrees C were 78 and 63%, being higher than those of WT (51%) and the other mutants (35-53%). S53D was stabilized with increase in the enthalpy change of activation for thermal inactivation while S65D was with decrease in the entropy change of activation. The stability of WT was enhanced by CaCl(2) and reached the level of S53D and S65D at 100 mM, suggesting that S53D and S65D might be stabilized by reinforcement of the Ca(2+)-binding structures.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Teisuke
authors.initials: T
authors.lastname: Takita
authors.forename: Takahiro
authors.initials: T
authors.lastname: Aono
authors.forename: Haruko
authors.initials: H
authors.lastname: Sakurama
authors.forename: Takafumi
authors.initials: T
authors.lastname: Itoh
authors.forename: Takumi
authors.initials: T
authors.lastname: Wada
authors.forename: Masashi
authors.initials: M
authors.lastname: Minoda
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yasukawa
authors.forename: Kuniyo
authors.initials: K
authors.lastname: Inouye
mesh_terms: D000179:Acrylates / Q000737:chemistry; D019943:Amino Acid Substitution; D001224:Aspartic Acid / Q000737:chemistry* / Q000235:genetics; D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002122:Calcium Chloride / Q000737:chemistry; D004151:Dipeptides / Q000737:chemistry; D004795:Enzyme Stability; D006868:Hydrolysis; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011487:Protein Conformation; D012694:Serine / Q000737:chemistry / Q000235:genetics; D013820:Thermolysin / Q000737:chemistry* / Q000235:genetics
chemical_list: D000179:Acrylates; D001426:Bacterial Proteins; D004151:Dipeptides; C108649:N-(3-(2-furyl)acryloyl)glycyl-leucinamide; D001224:Aspartic Acid; D012694:Serine; D013820:Thermolysin; D002122:Calcium Chloride