Induction of podoplanin by transforming growth factor-beta in human fibrosarcoma.
Podoplanin/aggrus is increased in tumors and its expression was associated with tumor malignancy. Podoplanin on cancer cells serves as a platelet-aggregating factor, which is associated with the metastatic potential. However, regulators of podoplanin remain to be determined. Transforming growth factor-beta (TGF-beta) regulates many physiological events, including tumorigenesis. Here, we found that TGF-beta induced podoplanin in human fibrosarcoma HT1080 cells and enhanced the platelet-aggregating-ability of HT1080. TGF-beta type I receptor inhibitor (SB431542) and short hairpin RNAs for Smad4 inhibited the podoplanin induction by TGF-beta. These results suggest that TGF-beta is a physiological regulator of podoplanin in tumor cells.
Suzuki
Hiroyuki
H
Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
Kato
Yukinari
Y
Kaneko
Mika Kato
MK
Okita
Yukari
Y
Narimatsu
Hisashi
H
Kato
Mitsuyasu
M
D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D005354:Fibrosarcoma / Q000378:metabolism* / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D010974:Platelet Aggregation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051901:Smad4 Protein / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D000098853:Podoplanin
D017931:DNA Primers; D008562:Membrane Glycoproteins; D051901:Smad4 Protein; D016212:Transforming Growth Factor beta; C117468:PDPN protein, human; D000098853:Podoplanin