title: [Peripheral blood p53 single nucleotide polymorphism analysis for early diagnosis of colorectal carcinoma].
abstract: OBJECTIVE
  To evaluate the role of p53 gene mutation in colorectal carcinoma and assess the value of peripheral blood p53 single nucleotide polymorphism (SNP) in early diagnosis of colorectal carcinoma.
  
  
  METHODS
  NSP in axons 5-8 of p53 gene was detected using ligase detection reaction-polymerase chain reaction (LDR-PCR) in the peripheral blood of 100 patients with colorectal cancer and 100 healthy subjects.
  
  
  RESULTS
  The mutation rate of p53 gene was 24% (24/120) in colorectal carcinoma patients and 0% (0/120) in the healthy subjects (P<0.05).
  
  
  CONCLUSIONS
  p53 plays an important role in the carcinogenesis of colorectal carcinoma, and SNP analysis for p53 gene can be helpful in early diagnosis of colorectal carcinoma.
authors.affiliation: Department of Gastroenterology, Meilin Hospital, Shenzhen 518049, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhuo-sheng
authors.initials: ZS
authors.lastname: Lai
authors.forename: Wei
authors.initials: W
authors.lastname: Wnag
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Miao
authors.initials: M
authors.lastname: Zhou
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000235:genetics; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D016159:Tumor Suppressor Protein p53