title: Functional tyrosine residue in the active center of human dipeptidyl peptidase III.
abstract: Abstract Human dipeptidyl peptidase III (DPP III) is a member of the metallopeptidase family M49 with an implied role in the pain-modulatory system and endogenous defense against oxidative stress. Here, we report the heterologous expression of human DPP III and the site-directed mutagenesis results which demonstrate a functional role for Tyr318 at the active site of this enzyme. The substitution of Tyr318 to Phe decreased kcat by two orders of magnitude without altering the binding affinity of substrate, or of a competitive hydroxamate inhibitor designed to interact with S1 and S2 subsites. The results indicate that the conserved tyrosine could be involved in transition state stabilization during the catalytic action of M49 peptidases.
authors.affiliation: Division of Molecular Biology, Ruder Bosković Institute, Bijenicka cesta 54, HR-10002 Zagreb, Croatia.
authors.forename: Branka
authors.initials: B
authors.lastname: Salopek-Sondi
authors.forename: Bojana
authors.initials: B
authors.lastname: Vukelić
authors.forename: Jasminka
authors.initials: J
authors.lastname: Spoljarić
authors.forename: Sumski
authors.initials: S
authors.lastname: Simaga
authors.forename: Dusica
authors.initials: D
authors.lastname: Vujaklija
authors.forename: Janja
authors.initials: J
authors.lastname: Makarević
authors.forename: Nina
authors.initials: N
authors.lastname: Jajcanin
authors.forename: Marija
authors.initials: M
authors.lastname: Abramić
mesh_terms: D001665:Binding Sites; D002384:Catalysis; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D014443:Tyrosine
chemical_list: D014443:Tyrosine; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C100152:dipeptidyl peptidase III