Differential expression of MYB gene (OgMYB1) determines color patterning in floral tissue of Oncidium Gower Ramsey.
The yellow coloration pattern in Oncidium floral lip associated with red sepal and petal tissues is an ideal model to study coordinate regulation of anthocyanin synthesis. In this study, chromatography analysis revealed that the red coloration in floral tissues was composed of malvidin-3-O-galactoside, peonidin-3-O-glucoside, delphinidin-3-O-glucoside and cyanidin-3-O-glucoside compounds. By contrary, these pigments were not detected in yellow lip tissue. Four key genes involved in anthocyanin biosynthetic pathway, i.e. chalcone synthase (OgCHS), chalcone isomerase (OgCHI), dihydroflavonol 4-reductase (OgDFR) and anthocyanidin synthase (OgANS) were isolated and their expression patterns were characterized. Northern blot analysis confirmed that although they are active during floral development, OgCHI and OgDFR genes are specifically down-regulated in yellow lip tissue. Bombardment with OgCHI and OgDFR genes into lip tissue driven by a flower-specific promoter, Pchrc (chromoplast-specific carotenoid-associated gene), demonstrated that transient expression of these two genes resulted in anthocyanin production in yellow lip. Further analysis of a R2R3 MYB transcription factor, OgMYB1, revealed that although it is actively expressed during floral development, it is not expressed in yellow lip tissue. Transient expression of OgMYB1 in lip tissues by bombardment can also induce formation of red pigments through the activation of OgCHI and OgDFR transcription. These results demonstrate that differential expression of OgMYB1 is critical to determine the color pattern of floral organ in Oncidium Gower Ramsey.
Chiou
Chung-Yi
CY
Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
Yeh
Kai-Wun
KW
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003116:Color; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000235:genetics*; D017343:Genes, Plant; D020570:Genes, myb; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000235:genetics*; D010858:Pigmentation; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D018749:RNA, Plant