Effects of silkworm hemolymph on cell viability and hCTLA4Ig production in transgenic rice cell suspension cultures.
Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could not elevate cell viability at the concentrations up to 3% (v/v), addition of 0.3% (v/v) SH to a culture medium enhanced the production of hCTLA4Ig by 36.8% over an SH-free medium. Moreover, the production period of hCTLA4Ig could be shortened in a 0.3% (v/v) SHadded medium compared with that in an SH-free culture. As a result, addition of 0.3% (v/v) SH improved the productivity of hCTLA4Ig significantly in transgenic rice cell cultures.
Cheon
Su-Hwan
SH
Department of Biological Engineering, Inha University, Incheon 402-751, Korea.
Lee
Kyoung-Hoon
KH
Kwon
Jun-Young
JY
Ryu
Hyun-Nam
HN
Yu
Da-Hyun
DH
Choi
Yong-Soo
YS
Kim
Dong-Il
DI
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis*; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D012831:Bombyx / Q000378:metabolism*; D060908:CTLA-4 Antigen; D002470:Cell Survival / Q000187:drug effects; D006458:Hemolymph / Q000378:metabolism*; D006801:Humans; D018796:Immunoconjugates / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011994:Recombinant Proteins / Q000378:metabolism
D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D018796:Immunoconjugates; D011994:Recombinant Proteins