[Extraction of sex hormone from antler velvet with supercritical CO2].
OBJECTIVE
To study the method of extraction of sex hormone from antler velvet with supercritical CO2.


METHODS
Supercritical fluid extraction (SFE) was used to extract sex hormone from antler velvet and radioimmunoassay (RIA) was used to analyze the extracts. The chemical compositions in extracts were identified by GC-MS, TLC and HPLC, respectively.


RESULTS
The experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 degrees C and extraction pressure of 30 MPa. Estradiol and progesterone in the extracts were 3.07, 776.18 ng x g(-1) respectively.


CONCLUSIONS
It is feasible to extract hormone from antler velvet with supercritical CO2.
Xu
Zhi-Hong
ZH
Key Laboratory for Green Chemical Technology of State Education Ministry, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China.
Li
Shu-Fen
SF
Wang
Jin-Yu
JY
Zhou
Ran
R
Tian
Song-Jiang
SJ
D000818:Animals; D000999:Antlers / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004958:Estradiol / Q000302:isolation & purification; D000431:Ethanol; D012739:Gonadal Steroid Hormones / Q000302:isolation & purification*; D008419:Materia Medica / Q000737:chemistry*; D011374:Progesterone / Q000302:isolation & purification; D011863:Radioimmunoassay
D012739:Gonadal Steroid Hormones; D008419:Materia Medica; D002245:Carbon Dioxide; D000431:Ethanol; D011374:Progesterone; D004958:Estradiol