title: The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with penicillin-binding protein 3, FtsW, and FtsN.
abstract: The monofunctional peptidoglycan glycosyltransferase (MtgA) catalyzes glycan chain elongation of the bacterial cell wall. Here we show that MtgA localizes at the division site of Escherichia coli cells that are deficient in PBP1b and produce a thermosensitive PBP1a and is able to interact with three constituents of the divisome, PBP3, FtsW, and FtsN, suggesting that MtgA may play a role in peptidoglycan assembly during the cell cycle in collaboration with other proteins.
authors.affiliation: Centre d'Ingénierie des Protéines, Université de Liège, Institut de Chimie, B6a, B-4000 Sart-Tilman (Liège), Belgium.
authors.forename: Adeline
authors.initials: A
authors.lastname: Derouaux
authors.forename: Benoît
authors.initials: B
authors.lastname: Wolf
authors.forename: Claudine
authors.initials: C
authors.lastname: Fraipont
authors.forename: Eefjan
authors.initials: E
authors.lastname: Breukink
authors.forename: Martine
authors.initials: M
authors.lastname: Nguyen-Distèche
authors.forename: Mohammed
authors.initials: M
authors.lastname: Terrak
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D046928:Peptidoglycan Glycosyltransferase / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C098711:FtsN protein, E coli; D008565:Membrane Proteins; D046915:Penicillin-Binding Proteins; D011993:Recombinant Fusion Proteins; C061684:FtsW protein, Bacteria; D049452:Green Fluorescent Proteins; D046928:Peptidoglycan Glycosyltransferase; C478681:penicillin-binding protein 1B, E coli; D046929:Serine-Type D-Ala-D-Ala Carboxypeptidase