Neurological impairment in rats after subarachnoid hemorrhage--a comparison of functional tests.
Functional outcome has become a key parameter for the determination of the efficacy of therapeutic interventions. Unfortunately, functional tests are not established for filament perforation induced subarachnoid hemorrhage (SAH). Therefore, we evaluated generally applied functional tasks for their potential to discriminate between various degrees of neuronal damage. Rats were subjected to SAH by an endovascular filament and were randomly assigned to controls treated with 0.9% NaCl, moderately neuroprotective therapy with 7.5% NaCl, and highly effective neuroprotection by 7.5% NaCl+6% dextran 70 (HSD). Functional deficit was quantified daily using beam balance task, prehensile traction task, rotarod, a 6-point motor function score and a general neurological 100-point score. Only the HSD group exhibited significantly more surviving neurons at postoperative day 7. Despite significant variations in histomorphometry, beam balance, prehensile traction and rotarod failed to distinguish between groups. On the other hand, the 100-point neuroscore showed improved neurological recovery on postoperative day 1 for HSD. The 100-point neuroscore failed to discriminate between treatment arms at later time points and therefore seems to reflect predominantly early neurological dysfunction. In conclusion, the results of pure motor tasks after experimental SAH in rats should be carefully interpreted. The integration of a test regimen to examine long term cognitive deficits after rat SAH might be valuable to gain additional information about the functional consequences of morphological damage.
Thal
Serge C
SC
Institute for Surgical Research, University of Munich Medical Center - Grosshadern, Munich, Germany. thal@uni-mainz.de
Mebmer
Konrad
K
Schmid-Elsaesser
Robert
R
Zausinger
Stefan
S
D000704:Analysis of Variance; D000818:Animals; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004195:Disease Models, Animal; D007839:Functional Laterality / Q000502:physiology; D017078:Laser-Doppler Flowmetry / Q000379:methods; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D009422:Nervous System Diseases / Q000209:etiology*; D009460:Neurologic Examination / Q000379:methods; D011597:Psychomotor Performance / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D045442:Rotarod Performance Test / Q000379:methods; D012720:Severity of Illness Index; D013345:Subarachnoid Hemorrhage / Q000150:complications*