Characterization of rotavirus strains detected among children and adults with acute gastroenteritis in Gizan, Saudi Arabia.
OBJECTIVE
To assess the circulating rotavirus strains among hospitalized children and adults in Gizan City.


METHODS
This cross-sectional study was based in 5 hospitals in the Gizan area. Stool samples were collected between November 2004 and March 2005, from sequential patients with acute, dehydrating diarrhea. Rotavirus antigen was detected in stool by enzyme-linked immunosorbent assay. The diversity of rotavirus strains was investigated using electropherotying and reverse transcription-polymerase chain reaction amplification of the VP7 and VP4 genes (G and P genotyping).


RESULTS
Rotavirus was detected in 54 of 454 (12%) subjects. The ages of those infected with rotavirus ranged from 15 days to 20 years, with a median age of 36 months. The highest rotavirus detection rate (24%) occurred in children aged 48-59 months. Overall, 50 (93%) of strains could be assigned both a G- and P-type; G1P[8] was the most frequently detected strain type (n=48, 89%) with one rotavirus each of G2P[4] and G9P[8].


CONCLUSIONS
Rotavirus strains circulating in Gizan would be well covered by current rotavirus vaccines. Rotavirus serotype G9 has been detected in Saudi Arabia for the first time. Continued surveillance of rotavirus strains is required.
Kheyami
Ali M
AM
Department of Medical Microbiology, The University of Liverpool, Duncan Building, Daulby Street, Liverpool, L69 3GA, United Kingdom. a.kheyami@liverpool.ac.uk
Areeshi
Mohammed Y
MY
Dove
Winifred
W
Nakagomi
Osamu
O
Cunliffe
Nigel A
NA
Anthony Hart
C
C
D000293:Adolescent; D000328:Adult; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D011159:Population Surveillance; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D012529:Saudi Arabia / Q000453:epidemiology; D014505:Urban Population