[A study on miRNA alternation after H2O2-induced PC12 cell apoptosis using microarray technique].
OBJECTIVE
To screen the differentially expressed microRNAs(miRNA) between H2O2-induced PC12 cell apoptosis and normal PC12 cells with microarray chips.


METHODS
PC12 cells were treated with different concentrations of H2O2 for 12 hours, and then the cell viabilities were evaluated by MTT assay and cell apoptosis rates were assessed by flow cytometry (FCM). miRNAs were isolated from control (0 nmol/L H2O2) and H2O2 treated (at concentrations of 50, 100, 200, and 400 nmol/L H2O2, respectively) groups of the PC12 cells, respectively, and were then detected and analyzed by microarray chips.


RESULTS
The P12 cell viabilities in control and H2O2 treated group were (92 +/- 9.8)%, (90 +/- 14.70)%, (80 +/- 13.85)%, (54 +/- 12.23)%, and (22 +/- 7.35)%, respectively. The apoptosis rates for them were 2.6%, 5.2%, 7.2%, 10.4%, 16.6%, and 72.2% accordingly. Expression of 68 miRNAs and 46 miRNAs were detected in both control group and the H2O2 treated groups. Of the 46 miRNAs in the H2O2 treated groups, 39 were similar to that of the control group and 6 were significantly down-regulated.


CONCLUSIONS
Our preliminary data may provide a new potential foundation for further study of pathogenesis and its treatment of nerve cell apoptosis caused by cerebral ischemia reperfusion.
Wang
Fen
F
Department of Forensic Medicine, Zhongshan school of Medicine, Sun Yat-sen University, Guangzhou 510080, China. wangfen_2004@163.com
Li
Zhao-hui
ZH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016716:PC12 Cells; D051381:Rats
D035683:MicroRNAs; D006861:Hydrogen Peroxide