Efficiency of two different transfection reagents for use with human NTERA2 cells.
The teratocarcinoma cell line NTERA2 is recently used in a wide range of researches (from developmental biology to toxicology, for their ability to be induced to neural differentiation. In order to study the genetic potential of these cells, it is needed to use methods for gene silencing and/or mRNA interference, allowing cell viability and further differentiation. To check these features, we simultaneously tested the transfection efficiency of NTERA2, A549 and HeLa cells with Metafectene PRO (Biontex, Germany) and another optimal transfection reagent currently used in our Laboratory, using as a reporter gene the DsRed2 vector (Clontech, Mountain View, CA). Under our culture conditions for NTERA2 and HeLa cells, Metafectene PRO transfection method was found to possess high throughput performance, that allows low concentration rate and low exposure time to excitation light source, thus reducing both toxicity and phototoxicity.
Aluigi
M G
MG
Department of Biology, University of Genova, Viale Benedetto XV, 5. I-16132 Genova, Italy.
Hofreiter
S
S
Falugi
C
C
Pestarino
M
M
Candiani
S
S
D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D005260:Female; D005562:Formazans / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000494:pharmacology; D008081:Liposomes / Q000737:chemistry* / Q000494:pharmacology; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology; D018243:Teratocarcinoma / Q000378:metabolism / Q000473:pathology*; D013778:Tetrazolium Salts / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D000097573:Red Fluorescent Protein
D004337:Drug Carriers; D005562:Formazans; D008055:Lipids; D008081:Liposomes; D008164:Luminescent Proteins; D013778:Tetrazolium Salts; D000097573:Red Fluorescent Protein; C500466:metafectene; C079782:MTT formazan