title: Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats.
abstract: BACKGROUND
  We have previously demonstrated differences in the gene expression of voltage-gated K v1.X channel alpha-subunits in arteries from Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs). The purpose of this study was to test the hypothesis that these differences are also present at the protein level.
  
  
  METHODS
  Proteins were isolated from the aorta, mesenteric (MAs) and tail arteries (TAs) of 12- to 15-week-old male WKY and SHR, and analyzed by immunoblotting. K(v) currents were recorded from MA myocytes by patch clamp methods.
  
  
  RESULTS
  Expression of Kv1.2, Kv1.5, and Kv2.1 was higher in MAs but was not different in aortas of SHRs as compared to WKYs. In the TA, expression of Kv1.2 and Kv1.5 was higher while that of Kv2.1 was lower in SHR compared to WKY. In the MA, the larger expression of an 80 kDa species of Kv1.2 in SHRs was associated with a lower expression of a 60 kDa species. Kv2.1 gene expression was larger in MAs from SHRs but not different in TAs. K(v) currents associated with Kv1.X and Kv2.1 channels were both larger in MA myocytes from SHRs but less than expected based upon differences in K(v) alpha-subunit protein expression.
  
  
  CONCLUSIONS
  For the MA, K(v) protein expression and current components between WKYs and SHRs were qualitatively consistent, but differences in gene and protein expression were not closely correlated. The higher expression of K(v) subunits in small mesenteric arteries (SMAs) of SHR would tend to maintain normal myogenic activity and vasoconstrictor reserve, and could be viewed as a form of homeostatic remodeling.
authors.affiliation: Department of Pharmacology, Lankenau Institute for Medical Research, Main Line Health System, Wynnewood, Pennsylvania, USA. coxr@mlhs.org
authors.forename: Robert H
authors.initials: RH
authors.lastname: Cox
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Fromme
authors.forename: Kimberly L
authors.initials: KL
authors.lastname: Folander
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Swanson
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology*; D007668:Kidney / Q000166:cytology; D051663:Kv1.2 Potassium Channel / Q000235:genetics / Q000276:immunology / Q000502:physiology; D051666:Kv1.5 Potassium Channel / Q000235:genetics / Q000276:immunology / Q000502:physiology; D008297:Male; D008638:Mesenteric Arteries / Q000502:physiology; D009000:Monocytes / Q000502:physiology; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics* / Q000276:immunology / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D051669:Shab Potassium Channels / Q000235:genetics / Q000276:immunology / Q000502:physiology; D013623:Tail / Q000098:blood supply; D013895:Thoracic Arteries / Q000502:physiology
chemical_list: C496991:Kcnb1 protein, rat; D051663:Kv1.2 Potassium Channel; D051666:Kv1.5 Potassium Channel; D024642:Potassium Channels, Voltage-Gated; D051669:Shab Potassium Channels