Cultivation of Plasmodium vivax.
Establishment of a continuous line of Plasmodium vivax parasite is crucial to understand the parasite's biology; however, this has not yet been achieved. Beginning in the 19th century, there were several efforts to cultivate this malaria parasite but without much success until the late 1980s. In addition, to date, only minor modifications of the methodology have been investigated, which has resulted in extending the cultivation period to around four weeks by supplying reticulocytes obtained from normal blood or rare hemochromatotic blood. However, the use of laboratory-produced erythroblasts to cultivate P. vivax enables maintenance of a continuous line of the parasite stably in the laboratory. Here, we summarize and compare the available methodologies and conditions for the in vitro cultivation of P. vivax.
Udomsangpetch
Rachanee
R
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand. scrud@mahidol.ac.th
Kaneko
Osamu
O
Chotivanich
Kesinee
K
Sattabongkot
Jetsumon
J
D000818:Animals; D002460:Cell Line; D003470:Culture Media; D046508:Culture Techniques; D004900:Erythroblasts / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D010274:Parasitology / Q000379:methods*; D010966:Plasmodium vivax / Q000254:growth & development*; D012156:Reticulocytes / Q000469:parasitology
D003470:Culture Media