title: The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure.
abstract: Cell adhesion and migration are strongly influenced by extracellular matrix (ECM) architecture and rigidity, but little is known about the concomitant influence of such environmental signals to cell responses, especially when considering cells of similar origin and morphology, but exhibiting a normal or cancerous phenotype. Using micropatterned polydimethylsiloxane substrates (PDMS) with tunable stiffness (500 kPa, 750 kPa, 2000 kPa) and topography (lines, pillars or unpatterned), we systematically analyse the differential response of normal (3T3) and cancer (SaI/N) fibroblastic cells. Our results demonstrate that both cells exhibit differential morphology and motility responses to changes in substrate rigidity and microtopography. 3T3 polarisation and spreading are influenced by substrate microtopography and rigidity. The cells exhibit a persistent type of migration, which depends on the substrate anisotropy. In contrast, the dynamic of SaI/N spreading is strongly modified by the substrate topography but not by substrate rigidity. SaI/N morphology and migration seem to escape from extracellular cues: the cells exhibit uncorrelated migration trajectories and a large dispersion of their migration speed, which increases with substrate rigidity.
authors.affiliation: LTM/CNRS/UMR5129, c/o CEA Grenoble, 17 rue des Martyrs, 38054 Grenoble cedex 9, France. tzvet@imag.fr <tzvet@imag.fr>
authors.forename: Tzvetelina
authors.initials: T
authors.lastname: Tzvetkova-Chevolleau
authors.forename: Angélique
authors.initials: A
authors.lastname: Stéphanou
authors.forename: David
authors.initials: D
authors.lastname: Fuard
authors.forename: Jacques
authors.initials: J
authors.lastname: Ohayon
authors.forename: Patrick
authors.initials: P
authors.lastname: Schiavone
authors.forename: Philippe
authors.initials: P
authors.lastname: Tracqui
mesh_terms: D000465:Algorithms; D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000494:pharmacology; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D007700:Kinetics; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D012828:Silicones / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon