Wild-type Leishmania donovani promastigotes block maturation, increase integrin expression and inhibit detachment of human monocyte-derived dendritic cells--the influence of phosphoglycans.
The protective immune response against the parasite, including the role of dendritic cells (DC) in the course of infection, plays a fundamental role. This study shows that wild-type (WT) Leishmania promastigotes and specifically the phosphoglycans family of virulence-associated antigens inhibit human monocyte-derived dendritic cells (MoDC) maturation and detachment to distinct surfaces. Immature phagocytosis of Leishmania donovani promastigotes by immature MoDC results in the increased expression of CD11b and CD51, and inhibition of cell detachment to distinct surfaces, which was dependent on the presence of phosphoglycans. These findings demonstrate that phosphoglycans of WT L. donovani might also inhibit human DC migration to lymphoid organs.
Tejle
Katarina
K
Department of Molecular and Clinical Medicine, Division of Medical Microbiology, Linköping University, Linköping, Sweden. katte@imk.liu.se
Lindroth
Margaretha
M
Magnusson
Karl-Eric
KE
Rasmusson
Birgitta
B
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D039481:CD11b Antigen / Q000096:biosynthesis; D002448:Cell Adhesion / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology*; D006801:Humans; D039564:Integrin alphaV / Q000096:biosynthesis; D016023:Integrins / Q000096:biosynthesis*; D007893:Leishmania donovani / Q000276:immunology*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009000:Monocytes / Q000276:immunology / Q000378:metabolism / Q000469:parasitology; D011134:Polysaccharides / Q000276:immunology*
D000953:Antigens, Protozoan; D039481:CD11b Antigen; C509295:ITGAM protein, human; D039564:Integrin alphaV; D016023:Integrins; D011134:Polysaccharides