Study of thiazole orange in aptamer-based dye-displacement assays.
We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.
Pei
Renjun
R
Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, NY 10032, USA. rp2164@columbia.edu
Stojanovic
Milan N
MN
D000255:Adenosine Triphosphate / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry*; D000242:Cyclic AMP / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D006160:Guanosine Triphosphate / Q000032:analysis; D009690:Nucleic Acid Conformation; D011804:Quinolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000032:analysis; D014031:Tobramycin / Q000032:analysis
D052157:Aptamers, Nucleotide; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011804:Quinolines; C051000:thiazole orange; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D013806:Theophylline; D000242:Cyclic AMP; D014031:Tobramycin