Fundamental studies on electrokinetic chromatography with PEGylated phospholipid micelles.
In this study, micelles prepared from distearoylphosphatidylethanolamine with covalently attached poly(ethylene) glycol) (PEG) of molecular weight 2000 (DSPE-PEG-2000) were employed in micellar electrokinetic chromatography (MEKC) as pseudostationary phases. Since DSPE-PEG-2000 contains long hydrophobic alkyl chains, an anionic phosphate group, and hydrophilic PEG chains, the prepared micelles are expected to provide a characteristic retention behavior for both neutral and ionic compounds. As a typical example, a baseline separation of phenol and 2-naphthol was successfully achieved by using the DSPE-PEG-2000 micelles as a background electrolyte for MEKC; such success clearly shows that the micelles can retain electrically neutral compounds. The MEKC separations of anionic and cationic compounds with a DSPE-PEG-2000 micellar solution and the enantioseparation of binaphthyl compounds with mixed micelles containing bile salt are also discussed.
Kitagawa
Fumihiko
F
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8510, Japan. fkitagawa@mbox.kudpc.kyoto-u.ac.jp
Hasegawa
Takayuki
T
Taji
Hiromi
H
Onoue
Satomi
S
Tsuda
Yoshiko
Y
Otsuka
Koji
K
D000465:Algorithms; D001647:Bile Acids and Salts / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004563:Electrochemistry; D007202:Indicators and Reagents; D007700:Kinetics; D008823:Micelles; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000737:chemistry*; D013237:Stereoisomerism
D001647:Bile Acids and Salts; D007202:Indicators and Reagents; D008823:Micelles; D010743:Phospholipids; D011092:Polyethylene Glycols