[Effect of a soluble Jagged 1/Fc chimera protein on the activation, proliferation and cell cycle of lymphocytes in mice].
OBJECTIVE
To investigate effect of a soluble Jagged 1/Fc chimera protein (Jagged 1/Fc) on activation, proliferation and cell cycles of lymphocytes in BALB/c mice.


METHODS
A model to evaluate the lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A (ConA), was established by a carboxy-fluorescein diacetate-succinimidyl ester (CFDA-SE)-labeling technique. Under an effective dose of 500 mug/L of Jagged 1/Fc, the effect of it on the lymphocyte proliferation was analyzed by flow cytometry. A propidium iodide-labeling technique was applied to estimate the influence of Jagged 1/Fc on the lymphocyte cell-cycle stimulated by phorbol 12,13-dibutyrate (PDB) plus Ionomycin (Ion). Expression levels of CD69 and CD25 molecules on surface of CD3(+) lymphocytes activated with ConA in the presence of Jagged 1/Fc were observed by a fluorescein-conjugated monoclonal antibody-labeling technique.


RESULTS
Jagged 1/Fc had no effect on the expression levels of CD69 and CD25 of the CD3(+) lymphocytes stimulated with or without ConA, and no effect on the proliferation index of the lymphocytes stimulated by ConA or PDB plus Ion. It led to the increase of sub-G0 phase cell proportion and the decrease of S phase cell proportion of the lymphocytes, but did not influence the changes of the lymphocyte cell-cycle induced by PDB plus Ion.


CONCLUSIONS
The results suggest that Jagged 1/Fc has no obvious effect on the activation and proliferation of lymphocytes in mice, but may promote the apoptosis of them, cause the G0/G1 phase arrest and block the S phase entry.
Xing
Fei-yue
FY
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China. tfyxing@jnu.edu.cn
Yu
Zhe
Z
Liu
Jing
J
Ji
Yu-hua
YH
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects* / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D000072100:Jagged-1 Protein; D037181:Lectins, C-Type / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015240:Phorbol 12,13-Dibutyrate; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology*; D000072099:Serrate-Jagged Proteins; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D002135:Calcium-Binding Proteins; D007141:Immunoglobulin Fc Fragments; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D037181:Lectins, C-Type; D008565:Membrane Proteins; D015240:Phorbol 12,13-Dibutyrate; D011994:Recombinant Proteins; D000072099:Serrate-Jagged Proteins; D000099192:CD69 Antigens; C000606274:Jag1 protein, mouse