[Effects of bone marrow-derived mesenchymal stem cells transplantation on recovery of neurological functions and expression of synaptophysin in focal cerebral infarction in rats].
OBJECTIVE
To investigate the effects of bone marrow-derived mesenchymal stem cells (BMSC) transplantation on the recovery of neurological functions and the expression of synaptophysin in focal cerebral infarction in rats.


METHODS
72 male adult Sprague-Dawley rats were randomly divided into 4 groups (18 in each group): shamoperated group, middle cerebral artery occlusion (MCAO) roup,vehicle group and MCAO+BMSC-treated group. A permanent focal cerebral ischemia model was established using modified Longa's method. BMSC was labeled by DAPI before the transplantation. One day after right middle cerebral artery occlusion(MCAO), 1 x 10(6) cells were injected into the lateral ventricle of rats in BMSCs-treated group and the same dose of PBS was given to the rats in vehicle group. Before sacrificed and at 4 d, 7 d and 14 d after MCAO, the neurological functions were tested by balance beam, rota-rod and screen prehensile and the synaptophysin was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical method.


RESULTS
DAPI stained positive cells were observed around the cerebral infarcted area in the BMSC-treated group. Compared with the MCAO group and the vehicle group,the neurological functions in BMSC-treated group were better on 7 d and 14 d after MCAO (P<0.05 or P<0.01), and the synaptophysin around the cerebral infarcted area was significantly upregulated on 4 d, 7 d and 14 d after MCAO (all P<0.01).


CONCLUSIONS
BMSC transplantation can improve the neurological functions by upregulating the expression of synaptophysin after MCAO in rats.
Weng
Jin-sen
JS
Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou 350001, China. wjs5227705@sina.com.cn
Liu
Nan
N
DU
Hou-wei
HW
Chen
Rong-hua
RH
Zhang
Yi-xian
YX
Wang
Jie-hua
JH
Huang
Hua-pin
HP
D000328:Adult; D000818:Animals; D002544:Cerebral Infarction / Q000235:genetics / Q000378:metabolism*; D005652:Functional Residual Capacity; D015870:Gene Expression; D006801:Humans; D008264:Macrophages; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016708:Synaptophysin / Q000235:genetics / Q000378:metabolism*
D016708:Synaptophysin