Growth phase-dependent changes in the expression of global regulatory genes and associated metabolic pathways in Escherichia coli.
Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fumC, acnA, sucA, acs and sodC, were increased (approximately 1.5 to 2-fold) as the cells entered the late phase of growth. The changes in the expression of other global regulators and their effects on different metabolic pathway genes were less significant, as compared to rpoS, during the later phases of growth.
Rahman
Mahbuba
M
Department of Bioscience and Bioinformatics, Faculty of Computer Science and System Engineering, Kyushu Institute of Technology, Iizuka, Fukuoka, 820-8502, Japan.
Hasan
Mohammad Rubayet
MR
Shimizu
Kazuyuki
K
D019342:Acetic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005809:Genes, Regulator; D005947:Glucose / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D012329:RNA, Bacterial / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012808:Sigma Factor / Q000378:metabolism*; D013997:Time Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D005947:Glucose; D019342:Acetic Acid