title: [Effect of Epstein-Barr virus reactivation on gene expression profile of nasopharyngeal carcinoma].
abstract: OBJECTIVE
  Epstein-Barr virus (EBV) infection plays a key role in the pathogenesis of nasopharyngeal carcinoma (NPC). This study was to explore the effects of the recurrent infection by EBV reactivation on the genomic expression profile of NPC.
  
  
  METHODS
  The microarray expression data from different cell lines subjected to primary infection of EBV+ vs. EBV- targets in NPC and recurrent EBV reactivation were collected from public data depository. Cross comparison, t-test analysis as well as filtering by flag, expression level and fold change were used to analyze the data and identify differential genes. Moreover, a set of web-based applications, such as DAVID (database for annotation, visualization and integrated discovery), pSTIING (protein, signaling, transcriptional interactions and inflammation networks gateway), GATHER (gene annotation tool to help explain relationships) and TELiS (transcription element listening system), were used to analyze and predict the probable expression profile of the differential genes.
  
  
  RESULTS
  As compared with the genes expressed during primary infection of EBV, 25 genes, including DUSP1, TOP1, HOXA9, DEK, PABPC1 and IMPDH2, were differentially expressed during EBV reactivation. Many of them were oncogenic. The differential genes together with related transcriptional factors were interacted mainly through 2 mechanisms: one mainly included TOP1, DUSP1, DUSP6, and RPS28; the other one was a circuit of PITX1, CD9, HOXA9 and IMPDH2.
  
  
  CONCLUSIONS
  The differential genes might participate in EBV reactivation by changing their expression level through two mechanisms, which contributes to the final development of NPC.
authors.affiliation: Institute of Genetic Engineering, Southern Medical University, Guangzhou, Guangdong, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Ma
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Liao
authors.forename: Tao
authors.initials: T
authors.lastname: Shang
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000472:pathogenicity; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods