Non-covalent capillary coatings for protein separations in capillary electrophoresis.
Adsorption of proteins, particularly basic proteins onto fused silica capillaries severely degrades capillary electrophoretic performance. This review provides a synopsis of the fundamentals underlying protein adsorption and its impact on CE performance. The efficacy of small molecule background electrolyte additives, surfactants, physically adsorbed polymers (dynamic and static), and successive multiple ionic-polymer layer coatings are evaluated using a number of performance metrics. Peak efficiency and migration time reproducibility are used as measures of reversible protein adsorption, while protein recovery, electroosmotic flow reproducibility and step changes in the baseline are used as indicators of irreversible protein adsorption.
Lucy
Charles A
CA
Department of Chemistry, University of Alberta, Gunning/Lemieux Chemistry Centre, Edmonton, AB T6G 2G2, Canada. charles.lucy@ualberta.ca
MacDonald
Amy M
AM
Gulcev
Makedonka D
MD
D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D000588:Amines / Q000737:chemistry; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000588:Amines; D016650:Fluorescein-5-isothiocyanate; D009211:Myoglobin; D010743:Phospholipids; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D011506:Proteins; D013501:Surface-Active Agents; D000099166:Polyacrylamides