Effect of bovine lactoferrin on extracellular matrix calcification by human osteoblast-like cells.
Osteoblast-mediated calcium deposition to the extracellular matrix (ECM) is a critical step in bone tissue generation. Bovine lactoferrin enhanced the calcium deposition by MG63 human osteoblast-like cells cultured on collagen-coated plates. Lactoferrin also promoted the alkaline phosphatase activity and osteocalcin production during the calcification process, whereas it had little effect on the growth of the cells on the collagen-coated plates.
Takayama
Yoshiharu
Y
Functional Biomolecules Research Team, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan. takay@affrc.go.jp
Mizumachi
Koko
K
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D000469:Alkaline Phosphatase; D007781:Lactoferrin