Overexpression of Shinorhizobium meliloti hemoprotein in Streptomyces lividans to enhance secondary metabolite production.
It was found that Shinorhizobium meliloti hemoprotein (SM) was more effective than Vitreoscilla hemoglobin (Vhb) in promoting secondary metabolites production when overexpressed in Streptomyces lividans TK24. The transformant with sm (sm-transformant) produced 2.7-times and 3-times larger amounts of actinorhodin than the vhbtransformant in solid culture and flask culture, respectively. In both solid and flask cultures, a larger amount of undecylprodigiocin was produced by the sm-transformant. It is considered that the overexpression of SM especially has activated the pentose phosphate pathway through oxidative stress, as evidenced by an increased NADPH production observed, and that it has promoted secondary metabolites biosynthesis.
Kim
Yoon Jung
YJ
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Sa
Soon Ok
SO
Chang
Yong Keun
YK
Hong
Soon-Kwang
SK
Hong
Young-Soo
YS
D000880:Anthraquinones / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006420:Hemeproteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D010427:Pentose Phosphate Pathway / Q000502:physiology; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism*; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic / Q000502:physiology; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D006420:Hemeproteins; D011353:Prodigiosin; D012329:RNA, Bacterial; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones