title: Neospora caninum and Toxoplasma gondii antibodies in dogs from Durango City, Mexico.
abstract: Toxoplasma gondii and Neospora caninum are structurally similar parasites, with many hosts in common. The prevalence of antibodies to T. gondii and N. caninum was determined in sera from dogs from Durango City, Mexico. Using a modified agglutination test, antibodies to T. gondii were found in 52 (51.5%) of the 101 dogs with titers of 1:25 in 27, 1:50 in 11, 1:100 in 5, 1:200 in 4, 1:400 in 2, 1:800 in 2, and 1:3,200 or higher in 1. Antibodies to N. caninum were determined by the indirect immunofluorescent antibody test (IFAT) and the Neospora sp. agglutination test (NAT). Two of the 101 dogs had N. caninum antibodies; these dogs did not have T. gondii antibodies, supporting the specificity of the tests used. The N. caninum antibody titers of the 2 dogs were: 1:400 by IFAT and 1:200 by NAT in 1, and 1:25 by NAT and IFAT in the other. Results indicate that these 2 structurally similar protozoans are antigenically different.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705-2350, USA. jitender.dubey@ars.usda.gov
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: C
authors.initials: C
authors.lastname: Alvarado-Esquivel
authors.forename: O
authors.initials: O
authors.lastname: Liesenfeld
authors.forename: R G
authors.initials: RG
authors.lastname: Herrera-Flores
authors.forename: B E
authors.initials: BE
authors.lastname: Ramírez-Sánchez
authors.forename: A
authors.initials: A
authors.lastname: González-Herrera
authors.forename: S A
authors.initials: SA
authors.lastname: Martínez-García
authors.forename: L A
authors.initials: LA
authors.lastname: Bandini
authors.forename: O C H
authors.initials: OC
authors.lastname: Kwok
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D003048:Coccidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology; D004285:Dogs; D005260:Female; D008297:Male; D008800:Mexico / Q000453:epidemiology; D018688:Neospora / Q000276:immunology*; D015995:Prevalence; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000453:epidemiology* / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan