title: Microparticle transport in the human intestinal M cell model.
abstract: The purpose of this study was to construct a human M cell model to evaluate the transport of polystyrene and albumin microparticles via this model. The integrity of the M cell model was evaluated by determining the transepithelial electrical resistance (TEER), and the alkaline phosphate activity was determined as an indicator of the presence of M-cell-like cells in the coculture. Both Fluoresbrite microparticles and albumin microparticles were transported by both Caco-2 monoculture and M cell models. The albumin microparticles showed a significantly greater transport via the M-cell-like cells compared to Caco-2 cells monoculture (p < 0.05). The coculture showed comparable TEER and reduction of 15-36% of alkaline phosphatase (AP) activity compared to the Caco-2 cell monoculture. This human M cell model was successfully constructed using 3 and 8 microm transwell inserts and it will serve as a useful tool in formulations screening and optimization of microparticle for oral vaccine delivery.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Mercer University, 3001 Mercer University Drive, Atlanta, GA 30341, USA. yinhwalai@gmail.com
authors.forename: Yin Hwa
authors.initials: YH
authors.lastname: Lai
authors.forename: Martin J
authors.initials: MJ
authors.lastname: D'Souza
mesh_terms: D000418:Albumins / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology*; D018938:Caco-2 Cells; D018920:Coculture Techniques; D017097:Electric Impedance; D005456:Fluorescent Dyes; D006801:Humans; D008954:Models, Biological; D053758:Nanoparticles; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism*; D011137:Polystyrenes; D014612:Vaccines / Q000008:administration & dosage
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes; D011137:Polystyrenes; D014612:Vaccines; D000469:Alkaline Phosphatase