Differential expression of two winter wheat alpha-tubulin genes during cold acclimation.
Overwintering crops, such as winter wheat, display significant increase in freezing tolerance during a period of cold acclimation (CA). To gain better understanding of molecular mechanisms of CA, it is important to unravel functions and regulations of CA-associated genes. Differential screening of a cDNA library constructed from cold-acclimated crown tissue of winter wheat identified an alpha-tubulin cDNA clone named wca18g11 that showed elevated expression upon cold acclimation. Nucleotide sequence analysis showed that the clone encoded a group 3 alpha-tubulin. Reverse transcription real-time PCR analysis of the expression of both wca18g11 clone and its closest paralogs of the wheat tubulin A-2 homeologous group during the course of cold acclimation revealed that both genes were differentially regulated with distinct expression patterns. The involvement of the two alpha-tubulin genes in cold acclimation and signal transduction is discussed.
Christov
Nikolai K
NK
AgroBioInstitute, Dragan Tsankov 8, Sofia 1164, Bulgaria. nikolai_christov@abi.bg
Imai
Ryozo
R
Blume
Yaroslav
Y
D000064:Acclimatization / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D016415:Sequence Alignment; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
D014404:Tubulin