[Changes of chlorine isotope composition characterize bacterial dehalogenation of dichloromethane].
Fractionation of dichloromethane (DCM) molecules with different chlorine isotopes by aerobic methylobacteria Methylobacterium dichloromethanicum DM4 and Albibacter nethylovorans DM10; cell-free extract of strain DM4; and transconjugant Methylobacterium evtorquens Al1/pME 8220, expressing the dcmA gene for DCM dehalogenase but unable to grow on DCM, was studied. Kinetic indices of DCM isotopomers for chlorine during bacterial dehalogenation and diffusion were compared. A two-step model is proposed, which suggests diffusional DCM transport to bacterial cells.
Ziakun
A M
AM
Firsova
Iu E
IuE
Torgonskaia
M L
ML
Doronina
N V
NV
Trotsenko
Iu A
IuA
D001673:Biodegradation, Environmental; D005591:Chemical Fractionation; D002713:Chlorine / Q000737:chemistry*; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism*; D054879:Halogenation; D007554:Isotopes; D008190:Lyases / Q000235:genetics / Q000378:metabolism; D008752:Methylene Chloride / Q000737:chemistry / Q000378:metabolism*; D020580:Methylobacterium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D042083:Methylocystaceae / Q000378:metabolism*
D004785:Environmental Pollutants; D007554:Isotopes; D002713:Chlorine; D008752:Methylene Chloride; D008190:Lyases; C045224:dichloromethane dehalogenase