Dietary omega-3 polyunsaturated fatty acids can inhibit expression of granzyme B, perforin, and cation-independent mannose 6-phosphate/insulin-like growth factor receptor in rat model of small bowel transplant chronic rejection.
BACKGROUND
The aim of our work was to investigate the effects of omega-3 polyunsaturated fatty acids on apoptosis and granzyme B, perforin, and cation-independent mannose 6-phosphate/insulin-like growth factor receptor (CI-MPR) expression of intestinal epithelial cells of chronic rejection after small intestinal transplantation.


METHODS
Small bowel transplantation was performed in a rat combination of 3 groups: group 1, Lewis-to-Lewis; group 2, F344-to-Lewis, dietary corn oil; group 3, F344-to-Lewis, dietary fish oil. All recipients were killed at 16 weeks posttransplantation. The apoptosis rate of mucosal cells was evaluated by flow cytometry. The expression of granzyme B, perforin, and CI-MPR was analyzed by reverse transcriptase PCR.


RESULTS
A high apoptotic rate was observed when the allografts demonstrated 1 or more histologic features of chronic rejection. omega-3 Polyunsaturated fatty acids decreased in rate of the apoptosis, and it can inhibit the expression of granzyme B, perforin, and CI-MPR.


CONCLUSIONS
omega-3 Polyunsaturated fatty acids can suppress the rejection to mucosal cells of allograft at the time of chronic rejection in small intestinal transplantation, which may be significant in increasing the surviving rate of allograft, delaying the chronic dysfunction, and prolonging the lifetime of both allograft and acceptor.
Kun
Zhao
Z
Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
Haiyun
Zhang
Z
Meng
Wang
W
Li Ning
Li Yousheng
Li Jieshou
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004195:Disease Models, Animal; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006084:Graft Rejection / Q000378:metabolism* / Q000473:pathology*; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D007421:Intestine, Small / Q000276:immunology / Q000637:transplantation*; D008297:Male; D054353:Perforin / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D017527:Receptor, IGF Type 2 / Q000378:metabolism; D017451:Receptors, Somatomedin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015525:Fatty Acids, Omega-3; D017527:Receptor, IGF Type 2; D017451:Receptors, Somatomedin; D054353:Perforin; D053804:Granzymes; C509215:Gzmb protein, rat