title: Association of MCP-1 and CCR2 polymorphisms with the risk of late acute rejection after renal transplantation in Korean patients.
abstract: Among the factors modulating transplant rejection, chemokines and their respective receptors deserve special attention. Increased expression of monocyte chemoattractant protein-1 (MCP-1) and its corresponding receptor (chemokine receptor-2, CCR2) has been implicated in renal transplant rejection. To determine the impact of the MCP-1-2518G and CCR2-64I genotypes on renal allograft function, 167 Korean patients who underwent transplantation over a 25-year period were evaluated. Genomic DNA was genotyped using polymerase chain reaction followed by restriction fragment length polymorphism analysis. Fifty-five (32.9%) patients were homozygous for the MCP-1-2518G polymorphism. Nine (5.4%) patients were homozygous for the CCR2-64I polymorphism. None of the investigated polymorphism showed a significant shift in long-term allograft survival. However, a significant increase was noted for the risk of late acute rejection in recipients who were homozygous for the MCP-1-2518G polymorphism (OR, 2.600; 95% CI, 1.125-6.012; P = 0.022). There was also an association between the MCP-1-2518G/G genotype and the number of late acute rejection episodes (P = 0.024). Although there was no difference in the incidence of rejection among recipients stratified by the CCR2-V64I genotype, recipients with the CCR2-V64I GG genotype in combination with the MCP-1-2518G/G genotype had a significantly higher risk of acute or late acute rejection among the receptor-ligand combinations (P = 0.006, P = 0.008, respectively). The MCP-1 variant may be a marker for risk of late acute rejection in Korean patients.
authors.affiliation: Department of Nephrology, College of Medicine, Inje University, Busan, South Korea.
authors.forename: S W
authors.initials: SW
authors.lastname: Kang
authors.forename: S J
authors.initials: SJ
authors.lastname: Park
authors.forename: Y W
authors.initials: YW
authors.lastname: Kim
authors.forename: Y H
authors.initials: YH
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Sohn
authors.forename: Y C
authors.initials: YC
authors.lastname: Yoon
authors.forename: H
authors.initials: H
authors.lastname: Joo
authors.forename: K H
authors.initials: KH
authors.lastname: Jeong
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: T W
authors.initials: TW
authors.lastname: Lee
authors.forename: C G
authors.initials: CG
authors.lastname: Ihm
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D018932:Chemokine CCL2 / Q000235:genetics*; D005260:Female; D006084:Graft Rejection / Q000235:genetics*; D006085:Graft Survival; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic; D054390:Receptors, CCR2 / Q000235:genetics*
chemical_list: C111835:CCL2 protein, human; C515334:CCR2 protein, human; D018932:Chemokine CCL2; D054390:Receptors, CCR2