title: A disease-specific fraction isolated from IVIG is essential for the immunosuppressive effect of IVIG in experimental autoimmune myasthenia gravis.
abstract: Intravenous immunoglobulin (IVIG) treatment is beneficially used in autoimmune disorders including myasthenia gravis (MG) although its mode of action and active components are still not fully identified. In an attempt to isolate from IVIG a disease-specific suppressive fraction, IVIG was passed on columns of IgG from rats with experimental autoimmune MG (EAMG) or from MG patients. These chromatographies resulted in depletion of the suppressive activity of IVIG on rat EAMG whereas the minute amounts of IgG fractions eluted from the EAMG- or MG-specific columns retained the immunosuppressive activity of IVIG. These results demonstrate that a minor disease-specific immunoglobulin fraction present in IVIG is essential for its suppressive activity.
authors.affiliation: Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel. sara.fuchs@weizmann.ac.il
authors.forename: Sara
authors.initials: S
authors.lastname: Fuchs
authors.forename: Tali
authors.initials: T
authors.lastname: Feferman
authors.forename: Roberto
authors.initials: R
authors.lastname: Meidler
authors.forename: Raanan
authors.initials: R
authors.lastname: Margalit
authors.forename: Camille
authors.initials: C
authors.lastname: Sicsic
authors.forename: Ningshan
authors.initials: N
authors.lastname: Wang
authors.forename: Kai-Yun
authors.initials: KY
authors.lastname: Zhu
authors.forename: Talma
authors.initials: T
authors.lastname: Brenner
authors.forename: Orgad
authors.initials: O
authors.lastname: Laub
authors.forename: Miriam C
authors.initials: MC
authors.lastname: Souroujon
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000302:isolation & purification* / Q000627:therapeutic use; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology; D002846:Chromatography, Affinity; D005620:Freund's Adjuvant / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016756:Immunoglobulins, Intravenous / Q000737:chemistry / Q000627:therapeutic use*; D007163:Immunosorbent Techniques; D007166:Immunosuppressive Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D009157:Myasthenia Gravis / Q000276:immunology; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000276:immunology / Q000628:therapy*; D051381:Rats; D013045:Species Specificity
chemical_list: D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous; D007166:Immunosuppressive Agents; C048844:anti-IgG; D005620:Freund's Adjuvant