The effect of local structural disruption on the yield of photochemical ligation between anthracene-oligonucleotide conjugates.
The techniques of chemical ligation have attracted great attention as an alternative to enzymatic joining of DNA ends. Here we introduce the photoligation of anthracene-modified ODN conjugates through anthracene cyclodimer formation. The effect of the positions and the kinds of single base mismatch on the template was evaluated using eight templates with one-base displacements. We found out that the yield of the ligation was affected by mispairing in a position-dependent manner. Such results would be attributed to the disruption of the local structure at the ligation site.
Arslan
Pelin
P
Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
Ihara
Toshihiro
T
Mukae
Motoko
M
Jyo
Akinori
A
D000873:Anthracenes / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D011088:DNA Ligases / Q000737:chemistry; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry
D000873:Anthracenes; D007202:Indicators and Reagents; D009841:Oligonucleotides; D011088:DNA Ligases