title: Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolated in Japan.
abstract: A part of the nucleotide sequence of the 5' untranslated region (5'UTR) and E rns region, and the genomic regions encoding for N pro, Core, and E2 of So-like isolates and IS25CP/01 strain which belong to bovine viral diarrhea virus genotype 1 (BVDV-1) were determined and genetic comparisons were made with sequences of other BVDV subgenotypes. Phylogenetic analysis using the 5'UTR and N pro revealed that So-like isolates and IS25CP/01 branched into independent phylogenetic branch. So-like isolates were clustered with Korean BVDV strains taken from DDBL/EMBL/GenBank in the 5'UTR. An additional two amino acid residues were found at the C terminal of the Core region of IS25CP/01. The similarity of amino acid sequence of E2 of So-like isolates and IS25CP/01 to the BVDV-1 reference strain NADL were 78.0-78.5 and 79.0, respectively. Cross-neutralization tests showed significant antigenic differences between So-like isolates and the others (Antigenic similarity (R) value: 2.2-8.8), and IS25CP/01 and the others (R value: 1.6-8.8). So-like viruses and IS25CP/01 differed from the thirteenth subgenotypes (1a-1m) reported by Jackova et al. (2007) and were classified as new genetic subtypes, BVDV-1n (So-like) and 1o (IS25CP/01).
authors.affiliation: Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Michiko
authors.initials: M
authors.lastname: Hayashi
authors.forename: Mika
authors.initials: M
authors.lastname: Itou
authors.forename: Toyoko
authors.initials: T
authors.lastname: Fukutomi
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology* / Q000821:virology; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D014644:Genetic Variation; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D016415:Sequence Alignment