Growth inhibition of breast cancer cell line MCF-7 by siRNA silencing of Wilms tumor 1 gene.
BACKGROUND
RNA interference (RNAi) is sequence-specific inhibition of gene expression induced by double-stranded RNA.


OBJECTIVE
Define the role of Wilms tumor 1 gene (WT1) in breast cancer oncogenesis using RNAi.


METHODS
MCF-7 breast cancer cells, which express a high level of WT1, were transfected with synthetic small interfering RNA (siRNA) targeting WT1 (siRNA(WT1)) resulting in inhibition of WTI expression, as well as growth, in a dose- and time-dependent manner.


RESULTS
The minimum concentration of siRNA(WT1) for growth inhibition and WT1 silencing was 25 nM and 50 nM respectively. WT1 expression was completely abolished at 200 nM siRNA,.


CONCLUSIONS
These data suggest that WTI1is indispensable for the survival of breast cancer MCF-7 cell line.
Navakanit
Ruxapon
R
Biomedical Sciences Department, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.
Graidist
Potchanapond
P
Leeanansaksiri
Wilairat
W
Dechsukum
Chavaboon
C
D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*
D019253:Proto-Oncogene Proteins c-bcl-2; D034741:RNA, Small Interfering