title: Methylibium subsaxonicum spec. nov., a betaproteobacterium Isolated from a hardwater rivulet.
abstract: A single strain, designated BF49(T), was isolated from a biofilm of a tufa deposit from the Westerhöfer rivulet, Lower Saxony, Germany. The G+C content of the genomic DNA of strain BF49(T) was 69 mol% and the predominant ubiquinone was Q-8. Major fatty acids were C(16:1)omega7c/15 iso 2OH and C(16:0). Comparative 16S rRNA gene sequence analysis indicated that the isolate was placed within the genus Methylibium, class Betaproteobacteria, distantly related to the type strain Methylibium petroleiphilum LMG 22953(T) (97.4% similarity), Methylibium fulvum Gsoil 322(T )(96%), and Methylibium aquaticum IMCC1728(T )(95.7%). On the basis of phylogenetic and phenotypic distinctness we propose a novel species, Methylibium subsaxonicum sp. nov., with strain BF49(T) (DSM 19570(T), CIP 109700(T)) as the type strain.
authors.affiliation: German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstr. Braunschweig, Germany. erko@dsmz.de
authors.forename: Erko
authors.initials: E
authors.lastname: Stackebrandt
authors.forename: Anja
authors.initials: A
authors.lastname: Frühling
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cousin
authors.forename: Evelyne
authors.initials: E
authors.lastname: Brambilla
authors.forename: Heinrich
authors.initials: H
authors.lastname: Lünsdorf
authors.forename: Susanne
authors.initials: S
authors.lastname: Verbarg
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification* / Q000648:ultrastructure; D018441:Biofilms; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D005858:Germany; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D025801:Ubiquitin / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D025801:Ubiquitin