Do oestrogens induce chromosome specific aneuploidy in vitro, similar to the pattern of aneuploidy seen in breast cancer?
The study was concerned with investigating the specific effects of non-DNA reactive oestrogens at low "biologically relevant" doses and the causative role they may play in breast cancer through inducing aneuploidy. A review of previous studies identified a non-random pattern of aneuploidy seen in breast cancers. This information was used to select those chromosomes that undergo copy number changes in breast cancer and chromosomes that appear stable. A panel of centromeric specific probes were selected and centromeric specific fluorescence in situ hybridisation (FISH) was carried out on the human lymphoblastoid cell line, AHH-1, which had been pre-treated with the chemical aneugens 17-beta oestradiol, diethylstilbestrol (DES) and bisphenol-A (BP-A). The results suggest that oestrogens may play a causative role in breast cancer by inducing a specific pattern of aneuploidy similar to that seen in breast carcinomas. 17-beta oestradiol appears to induce changes most similar to those seen in breast tumours, BP-A induces the same pattern but at a lower frequency and DES appears to be less chromosome specific in its act.
Quick
Emma L
EL
School of Medicine, University of Wales Swansea, Singleton Park, Swansea, Wales SA2 8PP, UK. emmalquick@hotmail.com
Parry
Elizabeth M
EM
Parry
James M
JM
D024422:Aneugens / Q000494:pharmacology; D000782:Aneuploidy; D001559:Benzhydryl Compounds; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D002869:Chromosome Aberrations / Q000187:drug effects; D004054:Diethylstilbestrol / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010636:Phenols / Q000494:pharmacology; D012196:Review Literature as Topic; D000098826:Bisphenol A Compounds
D024422:Aneugens; D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D004958:Estradiol; D004967:Estrogens; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds