title: Generation of a monoclonal antibody specific for tissue-nonspecific alkaline phosphatase and its use in a clinical diagnostic study.
abstract: Tissue-nonspecific alkaline phosphatase (TNSALP) in serum comprises liver alkaline phosphatase (liver-ALP) and bone alkaline phosphatase (bone-ALP). Liver-ALP is a marker of liver disease; thus a specific method for its measurement would be useful. Measurement of ALP by electrophoresis is difficult, although all of the isozymes can be assessed simultaneously. Total ALP can also be measured by automated analyzer, but it is difficult to determine the cause of a high ALP value because bone-, intestine-, placenta-, and tumor-ALP are measured together. Thus, anti-TNSALP monoclonal antibodies that can resolve these problems are needed. Here we have generated an anti-TNSALP monoclonal antibody, 3-29-3R. This clone has specificity to liver-ALP rather than to bone-ALP. In electrophoresis, 3-29-3R reacted with TNSALP and shifted the bands. The use of 3-29-3R enabled easy interpretation of the results. Furthermore, we tested 3-29-3R by developing an immunocapture enzymatic assay (IEA). Preliminary results of the IEA show that this method is effective for measurement of liver-ALP. Thus, the monoclonal antibody that we have established may be a useful tool for clinical diagnosis.
authors.affiliation: Nitto Boseki Corporation Ltd, Biochemical Laboratory, Koriyama, Japan. oohashit@jn.nittobo.co.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ohashi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sunaga
authors.forename: Toshihide
authors.initials: T
authors.lastname: Miura
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sasagawa
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sato
authors.forename: Wataru
authors.initials: W
authors.lastname: Ohashi
authors.forename: Ken
authors.initials: K
authors.lastname: Katagiri
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Katano
authors.forename: Iwao
authors.initials: I
authors.lastname: Kiyokawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Kojima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomonaga
authors.forename: Fumio
authors.initials: F
authors.lastname: Nomura
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
authors.forename: Kimimitsu
authors.initials: K
authors.lastname: Oda
authors.forename: Toyoji
authors.initials: T
authors.lastname: Sato
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Katayama
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000378:metabolism; D001859:Bone Neoplasms / Q000175:diagnosis / Q000201:enzymology* / Q000276:immunology; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas; D008107:Liver Diseases / Q000175:diagnosis / Q000201:enzymology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D009928:Organ Specificity / Q000276:immunology; D013379:Substrate Specificity / Q000276:immunology; D014018:Tissue Distribution / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase