[Quantitative analysis of adenosine and cordycepin in Cordyceps sinensis and its substitutes with LC-MS-MS].
OBJECTIVE
To develop a LC-MS-MS method for determination of adenosine and cordycepin in Cordyceps sinensis and it's substitutes.


METHODS
The sawple was extracted with. 90% methanol. Multi-reactions monitoring (MRM) technique was adopted.


RESULTS
The regression equations and coefficients were Y = 89.04X + 506.85 (r = 0.999 7) for adenosine, Y = 99.66X + 1 251.34 (r = 0.998 8) for cordycepin respectively. The linear range was 5.0-1 000.0 microg x L(-1) for adenosine and cordycepin. The limits of detection (LOD) were 0. 44 microg x L(-1) for adenosine and 0.31 microg x L(-1) for cordycepin, respectively. The average recoveries of adenosine and cordycepin were 98.1% and 97.9%, respectively.


CONCLUSIONS
The method was highly sensitive, selective and fast, which can be used for the determination of adenosine and cordycepin in C. sinensis and it's substitutes. This method can also be applied for the quality control of the medicinal materials.
Yang
Zhao
Z
Qingdao Institute for Drug Control, Qingdao 266071, China. yangzhao@yahoo.com.cn
Chi
Shao-Yun
SY
Zhang
Chun-Hui
CH
Wu
Aiying
A
D000241:Adenosine / Q000032:analysis* / Q000592:standards; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry* / Q000145:classification; D003839:Deoxyadenosines / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D003839:Deoxyadenosines; C058120:cordycepin; D000241:Adenosine