title: [Application of diatom detection using knead pulp method].
abstract: OBJECTIVE
  A novel technology for detection of diatom was discussed.
  
  
  METHODS
  Five grams of testing sample were taken and the organics were removed using simple mechanical knead pulp method. The homogenized samples were concentrated by centrifugation, smeared, and then examined under light microscope.
  
  
  RESULTS
  Except for a few feather's grains, the vast majority of diatom could be identified easily with clear diatom striations. The organic diatom could also be easily detected by this methodology.
  
  
  CONCLUSIONS
  The detection of diatom using knead pulp method is not only simple and inexpensive with a higher successful rate, but also causes nearly no harm to human and environment.
authors.affiliation: Department of Forensic Medicine, Wenzhou Medical College, Wenzhou 325035, China. yeghua345@126.com
authors.forename: Guang-hua
authors.initials: GH
authors.lastname: Ye
authors.forename: Lin-sheng
authors.initials: LS
authors.lastname: Yu
authors.forename: Yi-gu
authors.initials: YG
authors.lastname: Zhang
mesh_terms: D002118:Calcium / Q000378:metabolism; D017377:Diatoms / Q000302:isolation & purification* / Q000648:ultrastructure*; D004332:Drowning / Q000175:diagnosis*; D049429:Forensic Pathology; D006801:Humans; D007668:Kidney; D008099:Liver; D008168:Lung; D008853:Microscopy / Q000379:methods; D013048:Specimen Handling / Q000379:methods*; D014070:Tooth
chemical_list: D002118:Calcium