title: [Transformation of full-length gene of mouse/human chimeric antibody against Hantaan virus into Arabidopsis thaliana].
abstract: OBJECTIVE
  To construct the transgenic Arabidopsis thaliana containing full-length gene of mouse/human chimeric antibody(3G1MH) against Hantaan virus.
  
  
  METHODS
  The recombinant plasmid 3G1MH-pCAMBIA2301 was transformed into Agrobacterium tumefaciens GV3101 by TSS freeze-thaw method, and then the recombinant was transferred into wild Arabidopsis thaliana by vacuum-transgenic method. The regenerated transgenic plants were selected with kanamycin, and confirmed by PCR and Northern blot.
  
  
  RESULTS
  PCR result showed stable integration of the 3G1MH gene IN Arabidopsis thaliana genome in 7 stains of the transformed plants. Northern blot analyses confirmed the transcription of heavy and light chains in the transgenic plants.
  
  
  CONCLUSIONS
  The successful establishment of 3G1MH transgenic Arabidopsis thaliana plants pave the way for further research on expressing therapeutic antibody in transgenic plants.
authors.affiliation: Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Xing-an
authors.initials: XA
authors.lastname: Wu
authors.forename: Wen
authors.initials: W
authors.lastname: Luo
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.forename: Fang-lin
authors.initials: FL
authors.lastname: Zhang
authors.forename: Wen-tao
authors.initials: WT
authors.lastname: Bai
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.forename: Meng-yuan
authors.initials: MY
authors.lastname: Shi
authors.forename: Zhi-kai
authors.initials: ZK
authors.lastname: Xu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000276:immunology; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000276:immunology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D029681:Arabidopsis Proteins; D011994:Recombinant Proteins