Deletion analysis of the catalase-encoding gene (catB) promoter from Aspergillus oryzae.
The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using beta-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from -1,000 to -800 in the 1,400-bp catB promoter caused a drastic decrease in GUS activity. In addition, EMSA implicated a 45-bp element from -1,000 to -956 containing cis-elements. According to detailed promoter deletion analysis, a region from -1,000 to -975, which contains putative heat shock element (HSE) and the CCAAT-box, was involved in strong expression.
Hisada
Hiromoto
H
Research Institute, Gekkeikan Sake Co., Ltd., Kyoto, Japan. hisada@gekkeikan.co.jp
Sano
Motoaki
M
Hata
Yoji
Y
Abe
Yasuhisa
Y
Machida
Masayuki
M
D001236:Aspergillus oryzae / Q000201:enzymology* / Q000235:genetics*; D002374:Catalase / Q000235:genetics*; D017931:DNA Primers; D005656:Fungal Proteins / Q000235:genetics; D009154:Mutation; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion
D017931:DNA Primers; D005656:Fungal Proteins; D002374:Catalase