Purification of hydroxyanthraquinones and cinnamic acid from the roots of Rheum officinale Baill.
A chromatographic method for isolation and purification of chemical constituents from the well-known traditional Chinese drug Da-huang (roots of Rheum officinale Baill.) was established by using 12% cross-linked agarose gel, Superose 12, as the separation media. A two-step separation procedure is employed. Sixty five percent methanol was used as the eluent for separation of cinnamic acid, rhein, physcion and emodin form Da-huang crude extract. The fraction containing aloe-emodin and chrysophanol was then separated by using 55% methanol containing 0.5% acetic acid as the eluent. As a result, cinnamic acid and five kinds of hydroxyanthraquinones including rhein, aloe-emodin, chrysophanol, physcion and emodin were obtained. The retention behavior of hydroxyanthraquinones on Superose 12 was also studied. The retention of hydroxyanthraquinones on Superose 12 is based on a mixture of hydrogen bonding and hydrophobic interactions between the hydroxyl groups of the hydroxyanthraquinones and the residues of the cross-linking reagents used in the manufacturing process of Superose 12.
Huang
Yanyan
Y
College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, China.
Sun
Ailing
A
Qi
Yuanying
Y
Liu
Renmin
R
D000880:Anthraquinones / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000737:chemistry*; D012250:Rheum / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000880:Anthraquinones; D002934:Cinnamates; C029010:cinnamic acid