title: Serum resistin is reduced by glucose and meal loading in healthy human subjects.
abstract: Resistin is an adipokine that induces insulin resistance in mice; serum concentrations are decreased by fasting and increased by feeding. Adiponectin, another adipokine, improves insulin sensitivity. The aims of this study were to determine the effects of glucose and meal loading on serum resistin and total and high-molecular weight (HMW) adiponectin in humans and to explore potential determinants of fasting serum resistin and of changes in resistin. Serum resistin and total and HMW adiponectin were measured by enzyme-linked immunosorbent assay in young, lean, nondiabetic subjects during 75-g oral glucose tolerance test (OGTT) and meal tolerance test (MTT). Resistin single nucleotide polymorphism (SNP) -420 was typed. Serum resistin was decreased at 60 and 120 minutes during OGTT compared with baseline (n = 36, 1-way repeated-measures analysis of variance, P < .0001; Scheffe, P = .0457 and P < .0001, respectively). Serum resistin was also reduced at 240 minutes during MTT (n = 33, 1-way repeated measures analysis of variance, P < .0001; Scheffe, P = .0002). Multiple regression analysis adjusted for age, sex, and body mass index revealed that the reductions in serum resistin were dependent on baseline resistin levels. Subjects with greater baseline concentrations of resistin experienced more pronounced declines in resistin (OGTT, unstandardized regression coefficient (beta) = -0.19, P = .0005; MTT, beta = -0.63, P < .0001). Serum total and HMW adiponectin was unchanged. Fasting serum resistin was positively correlated with the G allele number of SNP -420 (beta = 7.70, P = .01) and white blood cell count (beta = 0.007, P = .0001) adjusted for age, sex, and body mass index. Therefore, in young, lean, nondiabetic humans, serum resistin was reduced by glucose and meal loading; the reduction in resistin was greater in subjects with higher fasting resistin. Fasting resistin was correlated with SNP -420 and white blood cell count.
authors.affiliation: Department of Molecular and Genetic Medicine, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Yamauchi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Osawa
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takasuka
authors.forename: Masaaki
authors.initials: M
authors.lastname: Ochi
authors.forename: Akiko
authors.initials: A
authors.lastname: Murakami
authors.forename: Wataru
authors.initials: W
authors.lastname: Nishida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Onuma
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Takata
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Tabara
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Miki
authors.forename: Jun
authors.initials: J
authors.lastname: Ohashi
authors.forename: Hideichi
authors.initials: H
authors.lastname: Makino
mesh_terms: D052242:Adiponectin / Q000097:blood; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002097:C-Reactive Protein / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004435:Eating / Q000502:physiology*; D005215:Fasting / Q000097:blood; D005260:Female; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D019518:Postprandial Period / Q000502:physiology*; D052243:Resistin / Q000097:blood* / Q000235:genetics
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D001786:Blood Glucose; D052243:Resistin; D002097:C-Reactive Protein; D004247:DNA; D003404:Creatinine