Toxoplasma gondii and Cryptosporidium parvum lack detectable DNA cytosine methylation.
Epigenetic factors play a role in the expression of virulence traits in Apicomplexa. Apicomplexan genomes encode putative DNA cytosine methylation enzymes. To assess the presence of cytosine methylation of Toxoplasma gondii and Cryptosporidium parvum DNA, we used mass spectrometry analysis and confirmed that these organisms lack detectable methylcytosine in their DNA.
Gissot
Mathieu
M
Albert Einstein College of Medicine, Ullmann 1225, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Choi
Sang-Woon
SW
Thompson
Reid F
RF
Greally
John M
JM
Kim
Kami
K
D044503:5-Methylcytosine / Q000032:analysis*; D000818:Animals; D016785:Cryptosporidium parvum / Q000235:genetics*; D019175:DNA Methylation; D016054:DNA, Protozoan / Q000378:metabolism; D013058:Mass Spectrometry; D014122:Toxoplasma / Q000235:genetics*
D016054:DNA, Protozoan; D044503:5-Methylcytosine