title: Characterizing the chromosomes of the platypus (Ornithorhynchus anatinus).
abstract: Like the unique platypus itself, the platypus genome is extraordinary because of its complex sex chromosome system, and is controversial because of difficulties in identification of small autosomes and sex chromosomes. A 6-fold shotgun sequence of the platypus genome is now available and is being assembled with the help of physical mapping. It is therefore essential to characterize the chromosomes and resolve the ambiguities and inconsistencies in identifying autosomes and sex chromosomes. We have used chromosome paints and DAPI banding to identify and classify pairs of autosomes and sex chromosomes. We have established an agreed nomenclature and identified anchor BAC clones for each chromosome that will ensure unambiguous gene localizations.
authors.affiliation: Comparative Genomics Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: McMillan
authors.forename: Pat
authors.initials: P
authors.lastname: Miethke
authors.forename: Amber E
authors.initials: AE
authors.lastname: Alsop
authors.forename: Willem
authors.initials: W
authors.lastname: Rens
authors.forename: Patricia
authors.initials: P
authors.lastname: O'Brien
authors.forename: Vladimir
authors.initials: V
authors.lastname: Trifonov
authors.forename: Frederic
authors.initials: F
authors.lastname: Veyrunes
authors.forename: Kyriena
authors.initials: K
authors.lastname: Schatzkamer
authors.forename: Colin L
authors.initials: CL
authors.lastname: Kremitzki
authors.forename: Tina
authors.initials: T
authors.lastname: Graves
authors.forename: Wesley
authors.initials: W
authors.lastname: Warren
authors.forename: Frank
authors.initials: F
authors.lastname: Grützner
authors.forename: Malcolm A
authors.initials: MA
authors.lastname: Ferguson-Smith
authors.forename: Jennifer A Marshall
authors.initials: JA
authors.lastname: Graves
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding; D002874:Chromosome Mapping; D020223:Chromosome Painting; D022202:Chromosomes, Artificial, Bacterial; D033481:Chromosomes, Mammalian / Q000235:genetics*; D005260:Female; D005347:Fibroblasts; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008677:Metaphase; D010987:Platypus / Q000235:genetics*; D012730:Sex Chromosomes / Q000235:genetics*