title: Identification of the archaeal alg7 gene homolog (encoding N-acetylglucosamine-1-phosphate transferase) of the N-linked glycosylation system by cross-domain complementation in Saccharomyces cerevisiae.
abstract: The Mv1751 gene product is thought to catalyze the first step in the N-glycosylation pathway in Methanococcus voltae. Here, we show that a conditional lethal mutation in the alg7 gene (N-acetylglucosamine-1-phosphate transferase) in Saccharomyces cerevisiae was successfully complemented with Mv1751, highlighting a rare case of cross-domain complementation.
authors.affiliation: Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
authors.forename: Hosam
authors.initials: H
authors.lastname: Shams-Eldin
authors.forename: Bonnie
authors.initials: B
authors.lastname: Chaban
authors.forename: Sebastian
authors.initials: S
authors.lastname: Niehus
authors.forename: Ralph T
authors.initials: RT
authors.lastname: Schwarz
authors.forename: Ken F
authors.initials: KF
authors.lastname: Jarrell
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics*; D005816:Genetic Complementation Test / Q000379:methods; D006031:Glycosylation; D017017:Methanococcus / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D020539:Sequence Analysis, Protein; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics*
chemical_list: D019843:Archaeal Proteins; D029701:Saccharomyces cerevisiae Proteins; D017855:Transferases (Other Substituted Phosphate Groups); C030012:UDP-N-acetylglucosamine-lysosomal-enzyme-N-acetylglucosaminephosphotransferase