title: [Effect of Qingkailing injection on rat CYP1A2 and CYP2D6 activity].
abstract: OBJECTIVE
  The present study investigates the influence of Qingkailing injection on rat liver CYP1A2 and CYP2D6 activity in vivo and in vitro, respectively.
  
  
  METHODS
  We employed HPLC to measure the metabolites of caffeine in the whole blood and calculated the ratio be between the metabolite and caffeine, which was used as index to evaluate the effect of Qingkailing injection on rat CYP1A2 activity in vivo; We also detected the CYP1A2 and CYP2D6 activity in microsomal reconstituted system by analysis of phenacetin metabolism and dextromethorphan metabolism with HPLC.
  
  
  RESULTS
  The metabolism of caffeine in treated groups was (15.9 +/- 3.8)%, (14.5 +/- 1.8)%, (12.3 +/- 1.2)%, with different concentration of Qingkailing injection (0.15, 0.3, 0.6 mL x kg(-1)) compared with (16.8 +/- 5.9)% in the control group, which was no significant difference among groups. In rat liver microsomal reconstituted system, Qingkailing injection has no inhibitory effect on CYP2D6 activity while the group with high dose has inhibitory effect on rat CYP1A2.
  
  
  CONCLUSIONS
  Qingkailing injection has no inhibitory effect on rat CYP1A2 and CYP2D6 in vivo and in vitro.
authors.affiliation: Institute of Pharmacy and Drug, The Second Affiliated Hospital of Harbin Medical University, Heilongjiang Province University Key Lab, Harbin 150086, China. liuyanhlj@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Bao
authors.forename: Wei Jiang
authors.initials: WJ
authors.lastname: Yu
authors.forename: Da-Ling
authors.initials: DL
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002110:Caffeine / Q000097:blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019388:Cytochrome P-450 CYP1A2 / Q000378:metabolism*; D019389:Cytochrome P-450 CYP2D6 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007267:Injections; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C504928:Qingkailing; D002110:Caffeine; D019388:Cytochrome P-450 CYP1A2; D019389:Cytochrome P-450 CYP2D6