Microparticle transport in the human intestinal M cell model.
The purpose of this study was to construct a human M cell model to evaluate the transport of polystyrene and albumin microparticles via this model. The integrity of the M cell model was evaluated by determining the transepithelial electrical resistance (TEER), and the alkaline phosphate activity was determined as an indicator of the presence of M-cell-like cells in the coculture. Both Fluoresbrite microparticles and albumin microparticles were transported by both Caco-2 monoculture and M cell models. The albumin microparticles showed a significantly greater transport via the M-cell-like cells compared to Caco-2 cells monoculture (p < 0.05). The coculture showed comparable TEER and reduction of 15-36% of alkaline phosphatase (AP) activity compared to the Caco-2 cell monoculture. This human M cell model was successfully constructed using 3 and 8 microm transwell inserts and it will serve as a useful tool in formulations screening and optimization of microparticle for oral vaccine delivery.
Lai
Yin Hwa
YH
Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Mercer University, 3001 Mercer University Drive, Atlanta, GA 30341, USA. yinhwalai@gmail.com
D'Souza
Martin J
MJ
D000418:Albumins / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology*; D018938:Caco-2 Cells; D018920:Coculture Techniques; D017097:Electric Impedance; D005456:Fluorescent Dyes; D006801:Humans; D008954:Models, Biological; D053758:Nanoparticles; D010581:Peyer's Patches / Q000166:cytology / Q000378:metabolism*; D011137:Polystyrenes; D014612:Vaccines / Q000008:administration & dosage
D000418:Albumins; D005456:Fluorescent Dyes; D011137:Polystyrenes; D014612:Vaccines; D000469:Alkaline Phosphatase