title: [Neuroprotective effects of vasoactive intestinal peptide against rat cerebral ischemia].
abstract: OBJECTIVE
  To explore the neuroprotective effect of vasoactive intestinal peptide (VIP) in rat ischemic brain injury.
  
  
  METHODS
  VIP was administered via intracerebroventricular injection in SD rats prior to focal cerebral ischemia by intraluminal occlusion of the middle cerebral artery. The infarct volume was assessed with TTC staining, and immunohistochemistry was performed to analyze the S100beta expression in the cerebral tissue, with the serum concentrations of S100beta detected by double-antibody sandwich enzyme-linked immunosorbent assay.
  
  
  RESULTS
  After VIP injection, the relative infarct volume in the rats with cerebral ischemia was significantly reduced by 32.3% as compared with the volume in the control group on day 1 (P<0.05), and the number of S100beta-positive cells was significantly decreased in the cerebral tissue (P<0.05). The injection also resulted in significantly decreased serum S100beta concentrations in the rats (P<0.05).
  
  
  CONCLUSIONS
  VIP injection can reduce the infarct volume in rats with focal cerebral ischemia, suggesting the neuroprotective effect of VIP in brain ischemia possibly by reducing S100beta overexpression.
authors.affiliation: Department of Human Anatomy and Histology and Embryology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. yangjie@mail.xjtu.edu.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Zhao-hua
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Yi-hua
authors.initials: YH
authors.lastname: Qian
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Hu
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Zong
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D002544:Cerebral Infarction / Q000517:prevention & control; D009414:Nerve Growth Factors / Q000097:blood; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D014660:Vasoactive Intestinal Peptide