Synthesis, characterization, DNA-binding study and anticancer properties of ternary metal(II) complexes of edda and an intercalating ligand.
A series of ternary metal(ii) complexes {M(phen)(edda); 1a (Cu), 1b (Co), 1c (Zn), 1d (Ni); H(2)edda = N,N(')-ethylenediaminediacetic acid} of N,N'-ethylene-bridged diglycine and 1,10-phenanthroline were synthesized and characterized by elemental analysis, FTIR, UV-visible spectroscopy and magnetic susceptibility measurement. The interaction of these complexes with DNA was investigated using CD and EPR spectroscopy. MTT assay results of 1a-1c , screened on MCF-7 cancer cell lines, show that synergy between the metal and ligands results in significant enhancement of their antiproliferative properties. Preliminary results from apoptosis and cell cycle analyses with flow cytometry are reported. seems to be able to induce cell cycle arrest at G(0)/G(1). The crystal structure of 1a is also included.
Ng
Chew Hee
CH
Faculty of Engineering and Science, Universiti Tunku Abdul Rahman, 53300, Kuala Lumpur, Malaysia. ngch@mail.utar.edu.my
Kong
King Chow
KC
Von
Sze Tin
ST
Balraj
Pauline
P
Jensen
Paul
P
Thirthagiri
Eswary
E
Hamada
Hirokazu
H
Chikira
Makoto
M
D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D008024:Ligands; D008297:Male; D008670:Metals / Q000737:chemistry*; D012474:Salmon
D000970:Antineoplastic Agents; D007364:Intercalating Agents; D008024:Ligands; D008670:Metals; D004247:DNA