Synthesis and biological activity of human neuropeptide S analogues modified in position 2.
Neuropeptide S (NPS) has been identified as the endogenous ligand of a previously orphan receptor now named NPSR. Previous studies demonstrated that the N-terminal sequence Phe (2)-Arg(3)-Asn(4) of the peptide is crucial for biological activity. Here we report on a focused structure-activity study of Phe(2) which has been replaced with a series of coded and noncoded amino acids. Thirty-one human NPS analogues were synthesized and pharmacologically tested for intracellular calcium mobilization by using HEK293 cells stably expressing the mouse NPSR. The results of this study demonstrated the following NPS position 2 structure-activity features: (i) lipophilicity but not aromaticity is crucial, (ii) both the size of the chemical moiety and its distance from the peptide backbone are important for biological activity, and (iii) this position plays a role in both receptor binding and activation, since [4,4'-biphenyl-Ala(2)]hNPS behaved as a partial agonist.
Camarda
Valeria
V
Department of Experimental and Clinical Medicine, Section of Pharmacology and Italian Institute of Neuroscience, University of Ferrara, via Fossato di Mortara 19, Ferrara, Italy.
Trapella
Claudio
C
Calo
Girolamo
G
Guerrini
Remo
R
Rizzi
Anna
A
Ruzza
Chiara
C
Fiorini
Stella
S
Marzola
Erika
E
Reinscheid
Rainer K
RK
Regoli
Domenico
D
Salvadori
Severo
S
D019943:Amino Acid Substitution; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D006801:Humans; D051379:Mice; D009479:Neuropeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010649:Phenylalanine / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
D009479:Neuropeptides; D043562:Receptors, G-Protein-Coupled; C506531:neuropeptide S, human; D010649:Phenylalanine; D002118:Calcium