Bacillus protein secretion: an unfolding story.
Bacillus subtilis and its close relatives are widely used for the production of enzymes for the detergent, food and beverage industries. These organisms not only produce an appropriate range of enzymes but also have the capacity to secrete them into the culture medium at high concentrations. Purification from the culture medium rather than from the cytoplasm considerably reduces downstream processing costs. In recent years, considerable effort has been aimed at developing B. subtilis as a host for the production of heterologous proteins. The folded state of the target protein at various stages of the secretion pathway has proved to be important.
Harwood
Colin R
CR
Institute of Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK. colin.harwood@ncl.ac.uk
Cranenburgh
Rocky
R
D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D007218:Industrial Microbiology; D018832:Molecular Chaperones / Q000378:metabolism; D017510:Protein Folding; D021382:Protein Sorting Signals; D021381:Protein Transport
D001426:Bacterial Proteins; D002413:Cations, Divalent; D018832:Molecular Chaperones; D021382:Protein Sorting Signals