Early postnatal development of the immune system in piglets: the redistribution of T lymphocyte subsets.
In this study, we have characterised postnatal changes in T lymphocyte subsets, especially gammadelta T lymphocytes, in blood, spleen and lymph nodes. Detection was carried out using two-colour flow cytometry and three-colour immunohistochemistry. During ontogeny, there was a significant increase in the total percentage of gammadelta T cells in the spleen and blood. In the lymph nodes, there were no age-dependent changes in the total percentage of gammadelta T cells, but the percentage of the gammadeltaTCR+CD8+ subpopulation significantly increased. The tissue distribution of gammadeltaTCR+CD8+ and gammadeltaTCR+CD8- cells in the lymph nodes is random and not collocated with a particular area of the organ. Furthermore, postnatal development was characterised by an increasing frequency of CD8+CD3+CD4-gammadeltaTCR-, which was compensated by a decreasing proportion of CD4+ lymphocytes. Double positive CD4+CD8+ lymphocytes were rare during the first month of life and a significant age-dependent increase of these cells was found in all the compartments monitored.
Stepanova
H
H
Department of Immunology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic.
Samankova
P
P
Leva
L
L
Sinkora
J
J
Faldyna
M
M
D000818:Animals; D000831:Animals, Newborn; D005434:Flow Cytometry; D007107:Immune System / Q000254:growth & development*; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000166:cytology / Q000254:growth & development / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D013552:Swine / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology
D016692:Receptors, Antigen, T-Cell, gamma-delta