title: Accuracy of herpes simplex virus detection in liquid-based (SurePath) Papanicolaou tests: a comparison with polymerase chain reaction.
abstract: A review of our institution's Papanicolaou test records over an 11-yr period showed that liquid-based Papanicolaou tests (LBPTs) had a significantly higher frequency of diagnoses of Herpes simplex virus (HSV)-related cellular changes compared to conventional Papanicolaou smears (77/302,841, 0.026% vs. 56/376,173, 0.015%, P = 0.002). To investigate the accuracy of the diagnosis of HSV by LBPT, we performed conventional polymerase chain reaction (PCR) on the residual samples from 258 prospectively collected LBPT and real-time PCR using a different primer set on a subset of 40 LBPT. Conventional PCR was positive in 22 of 22 cases diagnosed of HSV, 1 of 2 cases diagnosed as suspicious for HSV, and none of 234 LBPT without a cytologic HSV diagnosis. Real-time PCR was positive in 8 of 8 cases diagnosed as HSV and none of the 32 controls. We conclude that LBPT allows an increased detection of HSV that is highly accurate.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Fairview University Medical Center, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
authors.forename: Deniz L
authors.initials: DL
authors.lastname: Aslan
authors.forename: Stefan E
authors.initials: SE
authors.lastname: Pambuccian
authors.forename: Frances L
authors.initials: FL
authors.lastname: Prekker
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Schacker
authors.forename: Peter
authors.initials: P
authors.lastname: Southern
authors.forename: Kay
authors.initials: K
authors.lastname: Savik
authors.forename: Dan
authors.initials: D
authors.lastname: McKeon
authors.forename: H Evin
authors.initials: HE
authors.lastname: Gulbahce
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008137:Longitudinal Studies; D008403:Mass Screening; D008875:Middle Aged; D065006:Papanicolaou Test; D016133:Polymerase Chain Reaction / Q000379:methods*; D018139:Simplexvirus / Q000235:genetics*; D014621:Vagina / Q000473:pathology / Q000821:virology; D014626:Vaginal Smears / Q000379:methods*
chemical_list: D004279:DNA, Viral