Sperm deoxyribonucleic acid fragmentation as assessed by the sperm chromatin dispersion test in assisted reproductive technology programs: results of a large prospective multicenter study.
OBJECTIVE
To assess the value of sperm DNA fragmentation, measured by the sperm chromatin dispersion (SCD) test, in predicting fertilization rate, embryo quality, and pregnancy outcome.


METHODS
Prospective study.


METHODS
Four French infertility centers, from January to August 2005.


METHODS
Six hundred twenty-two couples participating in their first IVF or ICSI program.


METHODS
Analysis of DNA fragmentation by the sperm chromatin dispersion test in sperm samples used for IVF or ICSI.


METHODS
Correlations and associations between sperm parameters, sperm DNA integrity, and pregnancy outcomes.


RESULTS
A statistically significant correlation was observed between sperm DNA fragmentation rate and the following sperm characteristics: sperm motility, morphology, and concentration. We found a statistically significant relationship between sperm DNA fragmentation rate and fertilization rate, and we were able to suggest a threshold sperm DNA fragmentation rate of 18%, above which fragmentation rate was predictive of fertilization rate. Regarding embryo quality, we observed a relationship between sperm DNA fragmentation and embryo quality. No significant relationship was found between sperm DNA fragmentation rate and clinical pregnancies or births.


CONCLUSIONS
The results of this study confirm the utility of the sperm chromatin dispersion test for assessment of DNA fragmentation.
Velez de la Calle
Juan Felipe
JF
Unité In Vitro Fertilization, Clinique Pasteur Saint-Esprit, Brest, France.
Muller
Audrey
A
Walschaerts
Marie
M
Clavere
Jean Louis
JL
Jimenez
Clément
C
Wittemer
Christiane
C
Thonneau
Patrick
P
D000328:Adult; D048430:Cell Shape; D042002:Chromatin Assembly and Disassembly; D053938:DNA Fragmentation; D005260:Female; D005307:Fertilization in Vitro; D005602:France; D006801:Humans; D007246:Infertility / Q000473:pathology / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D011446:Prospective Studies; D015203:Reproducibility of Results; D055101:Semen Analysis / Q000379:methods*; D013076:Sperm Count; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*