Degradation of caspase-activated DNase by the ubiquitin-proteasome system.
DNA fragmentation is one of the most characteristic features of apoptotic cells and caspase-activated DNase (CAD) is considered to be a major nuclease responsible for DNA fragmentation. CAD forms a complex with its inhibitor (ICAD), which is also required for the functional folding of CAD, leading to CAD stabilization in cells. In this paper, we investigated the involvement of the ubiquitin-proteasome system in CAD stability. The expression and ubiquitination of CAD was remarkably increased by MG132 treatment in the absence of ICAD. These results suggest that CAD protein may be preferentially degraded by the ubiquitin-proteasome system in the absence of ICAD to maintain protein quality control.
Tsuruta
Tadamiki
T
Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Oh-hashi
Kentaro
K
Kiuchi
Kazutoshi
K
Hirata
Yoko
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D017895:Manganese Compounds / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D061988:Proteasome Inhibitors; D012402:Rotenone / Q000494:pharmacology; D019311:Staurosporine / Q000494:pharmacology; D019284:Thapsigargin / Q000494:pharmacology; D014162:Transfection; D025801:Ubiquitin / Q000378:metabolism*; D054875:Ubiquitination / Q000187:drug effects
D051017:Apoptosis Regulatory Proteins; D002712:Chlorides; D007976:Leupeptins; D017895:Manganese Compounds; D061988:Proteasome Inhibitors; D025801:Ubiquitin; C109664:caspase-activated DNase inhibitor; D012402:Rotenone; D019284:Thapsigargin; D003851:Deoxyribonucleases; C109663:caspase-activated deoxyribonuclease; C505409:Casp3 protein, mouse; D053148:Caspase 3; D046988:Proteasome Endopeptidase Complex; D019311:Staurosporine; C025340:manganese chloride; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde