title: Novel loop-mediated isothermal amplification method for detection of the JP2 clone of Aggregatibacter actinomycetemcomitans in subgingival plaque.
abstract: We developed a loop-mediated isothermal amplification method that detects the JP2 clone of Aggregatibacter actinomycetemcomitans, which induces aggressive periodontitis in adolescents of North and West African descents. Being independent of special equipment, this specific and sensitive method offers significant advantages for screening of patients on a population basis and in clinical settings.
authors.affiliation: Institute of Medical Microbiology and Immunology, Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Seki
authors.forename: Knud
authors.initials: K
authors.lastname: Poulsen
authors.forename: Dorte
authors.initials: D
authors.lastname: Haubek
authors.forename: Mogens
authors.initials: M
authors.lastname: Kilian
mesh_terms: D000189:Actinobacillus Infections / Q000382:microbiology*; D000293:Adolescent; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification*; D010520:Aggressive Periodontitis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D009018:Morocco; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial