title: Alpha-synuclein decreases arachidonic acid incorporation into rat striatal synaptoneurosomes.
abstract: In the present study we investigated the role of alpha-synuclein (ASN) and its fragment NAC in arachidonic acid (AA) turnover in the rat brain. Our data indicated that ASN (10 microM) inhibited [3H]AA incorporation into phosphatidylethanolamine (PE), phosphatidylcholine (PC) and phosphatidylserine (PS) together with phosphatidic acid (PA) by 13%, 27% and 38%, respectively. NAC (10 microM) reduced [3H]AA incorporation into PC and PA + PS by 17% and 34%, respectively. Because ASN and NAC lower the level of AA-CoA we suggest that inhibition of AA-CoA synthase is responsible for disturbed [3H]AA incorporation. In conclusion, ASN and NAC modulate phospholipid compositions in plasma membranes and affect synaptic endings function.
authors.affiliation: Department of Cellular Signalling, Medical Research Centre, Polish Academy of Sciences, 5 Pawiñskiego St., 02-106 Warsaw, Poland. agatazambrzycka@hotmail.com
authors.forename: Agata
authors.initials: A
authors.lastname: Adamczyk
authors.forename: Maria
authors.initials: M
authors.lastname: Kacprzak
authors.forename: Anna
authors.initials: A
authors.lastname: Kaźmierczak
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D051844:alpha-Synuclein / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D051844:alpha-Synuclein; D016718:Arachidonic Acid