A light-controlled reversible DNA photoligation via carbazole-tethered 5-carboxyvinyluracil.
We describe a light-controlled template-directed reversible DNA photoligation via carbazole-tethered 5-carboxyvinyluracil. Carbazole-tethered 5-carboxyvinyl-2'-deoxyuridine-containing oligodeoxynucleotide (ODN) can be ligated by irradiation at 366 nm in the presence of template ODN, and the ligated ODN can be split by irradiation at 366 nm in the absence of template ODN.
Fujimoto
Kenzo
K
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan. kenzo@jaist.ac.jp
Yoshino
Hideaki
H
Ami
Takehiro
T
Yoshimura
Yoshinaga
Y
Saito
Isao
I
D001483:Base Sequence; D002227:Carbazoles / Q000737:chemistry*; D004247:DNA / Q000528:radiation effects*; D008027:Light; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000528:radiation effects*; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry*
D002227:Carbazoles; D009838:Oligodeoxyribonucleotides; D014498:Uracil; D004247:DNA