title: Comparison of a lateral-flow immunochromatography assay with real-time reverse transcription-PCR for detection of human metapneumovirus.
abstract: A lateral-flow immunochromatography (IC) assay for the detection of human metapneumovirus (hMPV) has been developed by using two mouse monoclonal antibodies to the nucleocapsid protein of hMPV. The purpose of this study was to compare the virus detection rate in nasopharyngeal secretions by the IC assay with that by real-time reverse transcription-PCR (RT-PCR). We collected nasopharyngeal swab samples from 247 children with respiratory symptoms in Sapporo, Japan, during the period from April to July 2007. Sixty-eight of the 247 children were positive for hMPV by real-time RT-PCR. When the real-time RT-PCR was used as the reference standard, the IC assay results were positive for 48 of the 68 real-time RT-PCR-positive children (70.6% sensitivity) and 8 of the 179 real-time RT-PCR-negative children (95.5% specificity). Although the sensitivity of the IC assay is lower than that of real-time RT-PCR, the IC assay is a rapid and useful test for the diagnosis of hMPV infections in children.
authors.affiliation: Pediatric Clinic, Touei Hospital, N-41, E-16, Higashi-ku, Sapporo 007-0841, Japan. hide-ki@touei.or.jp
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kikuta
authors.forename: Chikako
authors.initials: C
authors.lastname: Sakata
authors.forename: Reiko
authors.initials: R
authors.lastname: Gamo
authors.forename: Akihito
authors.initials: A
authors.lastname: Ishizaka
authors.forename: Yasutsugu
authors.initials: Y
authors.lastname: Koga
authors.forename: Mutsuko
authors.initials: M
authors.lastname: Konno
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ogasawara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sawada
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Taguchi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Kazue
authors.initials: K
authors.lastname: Yasuda
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Ishiguro
authors.forename: Akio
authors.initials: A
authors.lastname: Hayashi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000911:Antibodies, Monoclonal; D002648:Child; D002675:Child, Preschool; D002845:Chromatography / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D029121:Metapneumovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D009305:Nasopharynx / Q000821:virology*; D019590:Nucleocapsid Proteins / Q000276:immunology; D018184:Paramyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D019590:Nucleocapsid Proteins