title: Investigation of the algT operon sequence in mucoid and non-mucoid Pseudomonas aeruginosa isolates from 115 Scandinavian patients with cystic fibrosis and in 88 in vitro non-mucoid revertants.
abstract: Pseudomonas aeruginosa is the dominant pathogen causing chronic lung infections in patients with cystic fibrosis (CF). After an initial phase characterized by intermittent colonizations, a chronic infection is established upon conversion of P. aeruginosa from the non-mucoid to the mucoid, alginate-overproducing phenotype. During the chronic infection the isolation of both mucoid and non-mucoid isolates in CF sputum samples is very common. The purpose of the present study was to establish, by sequence analysis, the types of mutations present in the algTmucABD operon in a large number of mucoid and non-mucoid P. aeruginosa isolates from Scandinavian CF patients and in in vitro-derived non-mucoid revertants. Mucoid (83) and non-mucoid isolates (103) from 91 Scandinavian patients with chronic P. aeruginosa infection and 24 non-mucoid isolates from intermittently colonized CF patients were investigated. In addition, 88 spontaneous non-mucoid revertants obtained in vitro from nine mucoid CF isolates were also included in the study. Mutations in mucA were found in 92 % of the mucoid and in up to 70 % of the non-mucoid isolates from chronically infected patients, indicating that the majority of non-mucoid isolates are revertants. None of the non-mucoid isolates from intermittently colonized CF patients harboured mucA mutations. Although algT has been considered an important gene for secondary-site mutations responsible for reversion to non-mucoidy, only 30 % of the mucA-mutated non-mucoid CF isolates had mutations in algT. In contrast, 83 % of the in vitro-derived spontaneous non-mucoid revertants had mutations in algT, showing that in the CF lung there is a selection for non-mucoid revertants with secondary-site mutations in genes other than algT. In addition, we report, to our knowledge for the first time, loss-of-function mutations in the negative regulators mucB and mucD in CF clinical isolates. In some of the CF isolates these mutations are associated with moderate alginate production. In conclusion, most non-mucoid isolates from chronically infected CF patients are revertants and the mechanism of revertance is algT-independent in the CF lung.
authors.affiliation: Institute of International Health, Immunology and Microbiology, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Oana
authors.initials: O
authors.lastname: Ciofu
authors.affiliation: Institute of International Health, Immunology and Microbiology, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Baoleri
authors.initials: B
authors.lastname: Lee
authors.affiliation: Uppsala CF Center, Department of Women's and Children's Health, Uppsala University, Uppsala University Hospital, SE-75185 Uppsala, Sweden.
authors.forename: Marie
authors.initials: M
authors.lastname: Johannesson
authors.affiliation: Department of Microbiology, Ullevaal University Hospital, Oslo, Norway.
authors.forename: Nils Olav
authors.initials: NO
authors.lastname: Hermansen
authors.affiliation: Lund CF Center, Children Hospital Lund, 221 85 Lund, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Meyer
authors.affiliation: Department of Clinical Microbiology, University Hospital, Rigshospitalet, Copenhagen, Denmark.|Institute of International Health, Immunology and Microbiology, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Høiby
mesh_terms: D000464:Alginates; D001426:Bacterial Proteins / Q000235:genetics*; D003550:Cystic Fibrosis / Q000150:complications*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020723:Glucuronic Acid / Q000096:biosynthesis / Q000235:genetics; D006603:Hexuronic Acids; D006801:Humans; D009154:Mutation; D009876:Operon / Q000235:genetics; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics*; D013548:Sweden
chemical_list: C079619:AlgU protein, Pseudomonas aeruginosa; D000464:Alginates; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D006603:Hexuronic Acids; C107895:MucA protein, Pseudomonas; C097648:MucC protein, Pseudomonas aeruginosa; D012808:Sigma Factor; D020723:Glucuronic Acid; C097649:MucD protein, Pseudomonas aeruginosa; D012697:Serine Endopeptidases