title: Borrelia burgdorferi complement regulator-acquiring surface protein 2 (CspZ) as a serological marker of human Lyme disease.
abstract: Serological diagnosis of Lyme disease may be complicated by antigenic differences between infecting organisms and those used as test references. Accordingly, it would be helpful to include antigens whose sequences are well conserved by a broad range of Lyme disease spirochetes. In the present study, line blot analyses were performed using recombinant complement regulator-acquiring surface protein 2 (BbCRASP-2) from Borrelia burgdorferi sensu stricto strain B31 and serum samples from human Lyme disease patients from throughout the United States and Germany. The results indicated that a large proportion of the patients had produced antibodies recognizing recombinant BbCRASP-2. In addition, Lyme disease spirochetes isolated from across North America and Europe were found to contain genes encoding proteins with high degrees of similarity to the B. burgdorferi type strain B31 BbCRASP-2, consistent with the high percentage of serologically positive patients. These data indicate that BbCRASP-2 may be valuable for use in a widely effective serological assay.
authors.affiliation: Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt am Main, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kraiczy
authors.forename: Annekatrin
authors.initials: A
authors.lastname: Seling
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Brissette
authors.forename: Evelyn
authors.initials: E
authors.lastname: Rossmann
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Hunfeld
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bykowski
authors.forename: Logan H
authors.initials: LH
authors.lastname: Burns
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Troese
authors.forename: Anne E
authors.initials: AE
authors.lastname: Cooley
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Miller
authors.forename: Volker
authors.initials: V
authors.lastname: Brade
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wallich
authors.forename: Sherwood
authors.initials: S
authors.lastname: Casjens
authors.forename: Brian
authors.initials: B
authors.lastname: Stevenson
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000276:immunology*; D005858:Germany; D006801:Humans; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D017422:Sequence Analysis, DNA; D014481:United States
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C440651:complement regulator-acquiring surface proteins, Borrelia burgdorferi