title: In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense, and Pochonia chlamydosporia on Ascaris suum eggs.
abstract: The in vitro effect of four isolates of the nematophagous fungi Duddingtonia flagrans (AC 001), Monacrosporium sinense (SF 53), and Pochonia chlamydosporia (VC 1 and VC 4) on eggs of Ascaris suum was evaluated. One hundred thousand A. suum eggs were plated on 2% water-agar with the grown isolates and control without fungus. After 7, 14, and 21 days, 100 eggs were removed and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, lytic effect with morphological alteration of embryo and eggshell; and type 3, lytic effect with morphological alteration of embryo and eggshell, besides hyphal penetration and internal egg colonization. P. chlamydosporia showed ovicidal activity (p < 0.01), mainly of the type 3 effect, on A. suum eggs in the studied intervals of 13.3% (isolate VC 1) and 17.3% (isolate VC 4), 13.9% (VC 1) and 17.7% (VC 4), and 19% (VC 1) and 20% (VC4), respectively, at 7, 14, and 21 days. The other fungi showed no type 3 effect. P. chlamydosporia is a potential biological control agent of A. suum eggs.
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG 36570-000, Brazil. jvictor@ufv.br
authors.forename: J V
authors.initials: JV
authors.lastname: Araújo
authors.forename: F R
authors.initials: FR
authors.lastname: Braga
authors.forename: A R
authors.initials: AR
authors.lastname: Silva
authors.forename: J M
authors.initials: JM
authors.lastname: Araujo
authors.forename: A O
authors.initials: AO
authors.lastname: Tavela
mesh_terms: D000818:Animals; D017165:Ascaris suum / Q000382:microbiology*; D001203:Ascomycota / Q000145:classification / Q000502:physiology*; D005260:Female; D010063:Ovum / Q000382:microbiology*; D010572:Pest Control, Biological; D013997:Time Factors