Conjugated polyelectrolytes for label-free DNA microarrays.
Classical strategies for gene microarrays require labeling of probes or target nucleic acids with signaling molecules, a process that is expensive, time consuming and not always reliable. Bazan and colleagues showed that a nucleic acid-binding cationic conjugated polyelectrolyte can be used in label-free DNA microarrays based on surfaces modified with neutral peptide nucleic acid (PNA) probes. This technique provides a simple and sensitive method for DNA detection without the need for covalent labeling of target DNA.
He
Fang
F
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, People's Republic of China.
Feng
Fude
F
Wang
Shu
S
D004573:Electrolytes / Q000737:chemistry*; D004867:Equipment Design; D017403:In Situ Hybridization / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods; D013194:Staining and Labeling
D004573:Electrolytes; D020135:Peptide Nucleic Acids; D011108:Polymers