title: Analysis of HIV type 1 gp41 sequences in diverse HIV type 1 strains.
abstract: The HIV fusion inhibitor enfuvirtide (ENF/Fuzeon) targets the env gp41 transmembrane domain. Mutations in gp41 are associated with ENF resistance. We developed a prototype assay to genotype a 676-bp region spanning the heptad repeat domains (HR1 and HR2) of HIV-1 gp41. Plasma samples were collected from 126 HIV-1-infected blood donors in Cameroon, Brazil, Uganda, South Africa, Thailand, and Argentina. Based on analysis of gag p24, pol integrase, and env gp41 genes, the panel was composed of subtypes A/A2 (18), B (11), C (14), D (10), F/F2 (9), G (7), CRF01_AE (9), CRF02_AG (33), and recombinant strains (15). Genotyping was successful for 119 of the 126 samples (94.4%). Although numerous amino acid polymorphisms were detected in some samples, none had primary mutations associated with ENF resistance. The gp41 HIV-1 research reagents developed by Celera are useful tools for genotyping analysis of the gp41 region in diverse HIV-1 strains.
authors.affiliation: Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA. seshlem@jhmi.edu
authors.forename: Susan H
authors.initials: SH
authors.lastname: Eshleman
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Hudelson
authors.forename: Robert
authors.initials: R
authors.lastname: Bruce
authors.forename: Travis
authors.initials: T
authors.lastname: Lee
authors.forename: Michelle R
authors.initials: MR
authors.lastname: Owens
authors.forename: John
authors.initials: J
authors.lastname: Hackett
authors.forename: Priscilla
authors.initials: P
authors.lastname: Swanson
authors.forename: Sushil G
authors.initials: SG
authors.lastname: Devare
authors.forename: Natalia
authors.initials: N
authors.lastname: Marlowe
mesh_terms: D001483:Base Sequence; D024882:Drug Resistance, Viral; D000077560:Enfuvirtide; D005838:Genotype; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D023581:HIV Fusion Inhibitors / Q000494:pharmacology; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D015700:HIV Envelope Protein gp41; D023581:HIV Fusion Inhibitors; D010446:Peptide Fragments; D000077560:Enfuvirtide