title: Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and apoptosis.
abstract: The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family of neutral sphingomyelinases that generates the bioactive sphingolipid ceramide. In this work the role of Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss of Isc1p resulted in a higher sensitivity to hydrogen peroxide that was associated with an increase in oxidative stress markers, namely intracellular oxidation, protein carbonylation, and lipid peroxidation. Microarray analysis showed that Isc1p deficiency up-regulated the iron regulon leading to increased levels of iron, which is known to catalyze the production of the highly reactive hydroxyl radicals via the Fenton reaction. In agreement, iron chelation suppressed hydrogen peroxide sensitivity of isc1Delta mutants. Cells lacking Isc1p also displayed a shortened chronological lifespan associated with oxidative stress markers and aging of parental cells was correlated with a decrease in Isc1p activity. The analysis of DNA fragmentation and caspase-like activity showed that Isc1p deficiency increased apoptotic cell death associated with oxidative stress and aging. Furthermore, deletion of Yca1p metacaspase suppressed the oxidative stress sensitivity and premature aging phenotypes of isc1Delta mutants. These results indicate that Isc1p plays an important role in the regulation of cellular redox homeostasis, through modulation of iron levels, and of apoptosis.
authors.affiliation: IBMC, Instituto de Biologia Molecular e Celular, Grupo de Microbiologia Celular e Aplicada, 4150-180 Porto, Portugal.
authors.forename: Teresa
authors.initials: T
authors.lastname: Almeida
authors.forename: Marta
authors.initials: M
authors.lastname: Marques
authors.forename: Dominik
authors.initials: D
authors.lastname: Mojzita
authors.forename: Maria A
authors.initials: MA
authors.lastname: Amorim
authors.forename: Rui D
authors.initials: RD
authors.lastname: Silva
authors.forename: Bruno
authors.initials: B
authors.lastname: Almeida
authors.forename: Pedro
authors.initials: P
authors.lastname: Rodrigues
authors.forename: Paula
authors.initials: P
authors.lastname: Ludovico
authors.forename: Stefan
authors.initials: S
authors.lastname: Hohmann
authors.forename: Pedro
authors.initials: P
authors.lastname: Moradas-Ferreira
authors.forename: Manuela
authors.initials: M
authors.lastname: Côrte-Real
authors.forename: Vítor
authors.initials: V
authors.lastname: Costa
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008954:Models, Biological; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D010738:Type C Phospholipases / Q000172:deficiency / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D007501:Iron; C419391:ISC1 protein, S cerevisiae; D010738:Type C Phospholipases; D020169:Caspases; C456272:MCA1 protein, S cerevisiae