[Neuroprotective effects of vasoactive intestinal peptide against rat cerebral ischemia].
OBJECTIVE
To explore the neuroprotective effect of vasoactive intestinal peptide (VIP) in rat ischemic brain injury.


METHODS
VIP was administered via intracerebroventricular injection in SD rats prior to focal cerebral ischemia by intraluminal occlusion of the middle cerebral artery. The infarct volume was assessed with TTC staining, and immunohistochemistry was performed to analyze the S100beta expression in the cerebral tissue, with the serum concentrations of S100beta detected by double-antibody sandwich enzyme-linked immunosorbent assay.


RESULTS
After VIP injection, the relative infarct volume in the rats with cerebral ischemia was significantly reduced by 32.3% as compared with the volume in the control group on day 1 (P<0.05), and the number of S100beta-positive cells was significantly decreased in the cerebral tissue (P<0.05). The injection also resulted in significantly decreased serum S100beta concentrations in the rats (P<0.05).


CONCLUSIONS
VIP injection can reduce the infarct volume in rats with focal cerebral ischemia, suggesting the neuroprotective effect of VIP in brain ischemia possibly by reducing S100beta overexpression.
Yang
Jie
J
Department of Human Anatomy and Histology and Embryology, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. yangjie@mail.xjtu.edu.cn
Zhao
Zhao-hua
ZH
Qian
Yi-hua
YH
Hu
Xiao-dan
XD
Li
Ming
M
Zong
Chang-hong
CH
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D002544:Cerebral Infarction / Q000517:prevention & control; D009414:Nerve Growth Factors / Q000097:blood; D018696:Neuroprotective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D014660:Vasoactive Intestinal Peptide