title: Expression and DNA binding activity of the tomato transcription factor RIN (ripening inhibitor).
abstract: Recently, we have found that the accumulation of ripening inhibitor (RIN) protein increased gradually during tomato fruit ripening. Here, the recombinant protein was expressed in Escherichia coli and affinity-purified. The DNA binding activity of renatured RIN protein was tested by electrophoretic mobility shift assay. The results indicated that an optimal expression and purification system was suitable for obtaining active RIN with DNA binding activity.
authors.affiliation: Laboratory of Fruit Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Qinghua Donglu, Beijing, PR China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Zhu
authors.forename: Ben-Zhong
authors.initials: BZ
authors.lastname: Zhu
authors.forename: Ying-Cong
authors.initials: YC
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Shao
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Wang
authors.forename: Yuan-Hong
authors.initials: YH
authors.lastname: Xie
authors.forename: An-Jun
authors.initials: AJ
authors.lastname: Chen
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Luo
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Jia
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Luo
mesh_terms: D018744:DNA, Plant / Q000235:genetics* / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018551:Solanum lycopersicum / Q000378:metabolism*; D026161:MADS Domain Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D018744:DNA, Plant; D004268:DNA-Binding Proteins; D026161:MADS Domain Proteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D012097:Repressor Proteins; D014157:Transcription Factors