title: Use of sequence analysis of the VP4 gene to classify recent Vietnamese rotavirus isolates.
abstract: Twenty-eight strains of P(8), four of P(4) and one of P(19) rotavirus, isolated in Ho Chi Minh City, Vietnam, during 2002-2003, were investigated by sequence analysis of the VP4 gene. Seven of the 28 P(8) rotavirus VP4 sequences clustered in the P(8)-3 lineage, or the rare, so-called OP354-like lineage. Amino-acid sequence comparison revealed that Vietnamese P(8)-3 rotaviruses were generally very similar to Malawian strains, including the prototype OP354 strain. The numerical severity scores of diarrhoeal disease caused by the Vietnamese P(8)-3 rotaviruses were statistically higher than those of diarrhoeal disease caused by rotaviruses in the more common P(8)-2 lineage. Sequence and phylogenetic analysis of the VP4 gene of a Vietnamese G9P(19) rotavirus isolate showed a high degree of homology with the cognate genes of other human and porcine rotaviruses, including the prototype 4F strain.
authors.affiliation: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: T A
authors.initials: TA
authors.lastname: Nguyen
authors.forename: L P
authors.initials: LP
authors.lastname: Hoang
authors.forename: L D
authors.initials: LD
authors.lastname: Pham
authors.forename: K T
authors.initials: KT
authors.lastname: Hoang
authors.forename: S
authors.initials: S
authors.lastname: Okitsu
authors.forename: M
authors.initials: M
authors.lastname: Mizuguchi
authors.forename: H
authors.initials: H
authors.lastname: Ushijima
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D002675:Child, Preschool; D016000:Cluster Analysis; D003967:Diarrhea / Q000473:pathology / Q000821:virology; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000821:virology*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D012720:Severity of Illness Index; D014744:Vietnam
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus