title: [Extraction of sex hormone from antler velvet with supercritical CO2].
abstract: OBJECTIVE
  To study the method of extraction of sex hormone from antler velvet with supercritical CO2.
  
  
  METHODS
  Supercritical fluid extraction (SFE) was used to extract sex hormone from antler velvet and radioimmunoassay (RIA) was used to analyze the extracts. The chemical compositions in extracts were identified by GC-MS, TLC and HPLC, respectively.
  
  
  RESULTS
  The experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 degrees C and extraction pressure of 30 MPa. Estradiol and progesterone in the extracts were 3.07, 776.18 ng x g(-1) respectively.
  
  
  CONCLUSIONS
  It is feasible to extract hormone from antler velvet with supercritical CO2.
authors.affiliation: Key Laboratory for Green Chemical Technology of State Education Ministry, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Xu
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Li
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Wang
authors.forename: Ran
authors.initials: R
authors.lastname: Zhou
authors.forename: Song-Jiang
authors.initials: SJ
authors.lastname: Tian
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004958:Estradiol / Q000302:isolation & purification; D000431:Ethanol; D012739:Gonadal Steroid Hormones / Q000302:isolation & purification*; D008419:Materia Medica / Q000737:chemistry*; D011374:Progesterone / Q000302:isolation & purification; D011863:Radioimmunoassay
chemical_list: D012739:Gonadal Steroid Hormones; D008419:Materia Medica; D002245:Carbon Dioxide; D000431:Ethanol; D011374:Progesterone; D004958:Estradiol