title: Preservation of hematopoietic stem and progenitor cells from umbilical cord blood stored in a surface derivatized with polymer nanosegments.
abstract: Tissue culture flasks were prepared with immobilized amphiphilic nanosegments of Pluronic F68 and F127, polyethylene oxide (PEO)-polypropylene oxide (PPO)-PEO triblock copolymers, on their surfaces. These so-called "Pluronic-immobilized flasks" were used for the preservation of hematopoietic stem and progenitor cells from umbilical cord blood. The expression ratio of surface markers (CD34) on hematopoietic stem and progenitor cells stored in Pluronic-immobilized flasks was significantly higher than that in polystyrene tissue culture flasks or commercially available bioinert flasks (i.e., low cell-binding cultureware). This was due to the presence of flexible brushlike segments of Pluronic on the Pluronic-immobilized flask. A good correlation was found between the number of CD34+ cells and the ratio of viable CD34+ cells from cord blood in several flasks after five days of storage. Therefore, the high number of CD34+ cells was thought to have originated from the high viability of these cells stored in Pluronic-immobilized flasks. It was found that there was an optimal surface concentration of Pluronic on the Pluronic-immobilized flask surfaces for the preservation (high number and survival) of these stem and progenitor cells. The foregoing results were attributable to the high density of Pluronic nanosegments on the flask surface, limiting the movement of these flexible segments.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, 32001 Taiwan. higuchi@ncu.edu.tw
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
authors.forename: Wen-Yih
authors.initials: WY
authors.lastname: Chen
authors.forename: Taro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Gomei
authors.forename: Hisashi
authors.initials: H
authors.lastname: Fukushima
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
authors.forename: Ruoh-chyu
authors.initials: RC
authors.lastname: Ruaan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D051379:Mice; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D011309:Preservation, Biological / Q000379:methods*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties / Q000187:drug effects
chemical_list: D011108:Polymers