Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors.
Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.
Cavalcante
Roberta Barroso
RB
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Lopes
Fernanda Ferreira
FF
Ferreira
Andréa Soares
AS
Freitas
Roseana de Almeida
Rde A
de Souza
Lélia Batista
LB
D000199:Actins / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008840:Microfilament Proteins / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009208:Myoepithelioma / Q000378:metabolism* / Q000473:pathology; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D014746:Vimentin / Q000378:metabolism; D000096985:Calponins
D000199:Actins; D014408:Biomarkers, Tumor; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D014746:Vimentin; D000096985:Calponins