title: High glucose oxidizes SERCA cysteine-674 and prevents inhibition by nitric oxide of smooth muscle cell migration.
abstract: Nitric oxide (NO) causes S-glutathiolation of the reactive cysteine-674 in the sarcoplasmic/endoplasmic reticulum Ca(2+) ATPase (SERCA), thus increasing SERCA activity, and inhibiting Ca(2+) influx and migration of vascular smooth muscle cells (VSMC). Because increased VSMC migration contributes to accelerated neointimal growth and atherosclerosis in diabetes, the effect of culture of VSMC in high glucose (HG) was determined. Rat aortic VSMC were exposed to normal (5.5 mmol/L) or high (25 mmol/L) glucose for 3 days, and serum-induced cell migration during 6 h into a wounded cell monolayer was measured 5 min after adding the NO donor S-nitroso-N-acetylpenicillamine (SNAP) or 24 h after interleukin-1beta (IL-1beta) to express inducible nitric oxide synthase (iNOS). In normal glucose, SNAP or IL-1beta significantly inhibited migration in cells infected with adenovirus to express GFP or SERCA wild type (WT), but not with a C674S SERCA mutant. After HG, NO failed to inhibit migration, nor did it decrease calcium-dependent association of calmodulin with calcineurin, indicating that NO failed to decrease intracellular calcium levels via SERCA. In contrast, overexpression of SERCA WT, but not the SERCA C674S mutant, preserved the ability for NO to inhibit migration despite exposing the cells to HG. The antioxidant, Tempol, or overexpression of superoxide dismutase also prevented the effects of HG. Further studies showed that both biotinylated-iodoacetamide and NO-induced biotinylated glutathione labeling of SERCA C674 were decreased by HG, and a sequence-specific sulfonic acid antibody detected oxidation of the C674 SERCA thiol. These results indicate that failure of NO to inhibit migration in VSMC exposed to HG is due to oxidation of the SERCA reactive cysteine-674.
authors.affiliation: Vascular Biology Unit, Whitaker Cardiovascular Institute, Boston University School of Medicine, X720, 650 Albany Street, Boston, MA 02118, USA. xytong@bu.edu
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Tong
authors.forename: Jia
authors.initials: J
authors.lastname: Ying
authors.forename: David R
authors.initials: DR
authors.lastname: Pimentel
authors.forename: Mario
authors.initials: M
authors.lastname: Trucillo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Adachi
authors.forename: Richard A
authors.initials: RA
authors.lastname: Cohen
mesh_terms: D000818:Animals; D019921:Biotinylation; D019703:Calcineurin / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects*; D003497:Cyclic N-Oxides / Q000494:pharmacology; D003545:Cysteine / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007460:Iodoacetamide; D008358:Mannose / Q000494:pharmacology; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D026423:S-Nitroso-N-Acetylpenicillamine / Q000494:pharmacology; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000378:metabolism*; D013113:Spin Labels; D013451:Sulfonic Acids / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002147:Calmodulin; D003497:Cyclic N-Oxides; D020030:Nitric Oxide Donors; D013113:Spin Labels; D013451:Sulfonic Acids; D009569:Nitric Oxide; D026423:S-Nitroso-N-Acetylpenicillamine; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase; D019703:Calcineurin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D005978:Glutathione; D005947:Glucose; D003545:Cysteine; D008358:Mannose; D002118:Calcium; C001803:tempol; D007460:Iodoacetamide