title: Emergence of complex rearrangements at translocation breakpoints in a transgenic mouse; implications for mechanisms involved in the formation of chromosome rearrangements.
abstract: Cryptic complex rearrangements as a result of a reciprocal chromosome translocation have been characterised in a transgenic mouse strain. Analysis of the breakpoint junctions in our previous studies showed that the ada transgene was integrated at the breakpoint forming a fusion gene with Golga3 (Mea2). In this study, further detailed analysis around the translocation junctions revealed that the surrounding regions were composed of 13 fragments of defined transgenic chromosome origins over approximately 1.9-Mb areas. Exactly the same cluster structure of these 13 breakpoint fragments already existed in the second generation of the transgenic mice. Our results show that this highly complex rearrangement has been conserved as the incipient form without any additional changes for 18 years up to the present generation, suggesting simultaneous occurrence of multiple events in the founder mouse.
authors.affiliation: Division of Molecular Pathology and Genetics, Kanagawa Cancer Center Research Institute, Yokohama, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Kasai
authors.forename: M
authors.initials: M
authors.lastname: Yoshihara
authors.forename: S
authors.initials: S
authors.lastname: Matsukuma
authors.forename: P
authors.initials: P
authors.lastname: O'Brien
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson-Smith
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics; D001483:Base Sequence; D019457:Chromosome Breakage; D002875:Chromosomes / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000235:genetics; D017353:Gene Deletion; D015321:Gene Rearrangement / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D005810:Multigene Family; D016415:Sequence Alignment
chemical_list: D001324:Autoantigens; C512726:Golga3 protein, mouse; D008565:Membrane Proteins; C400322:cytochrome P-450 CYP2C subfamily; D004247:DNA; D003577:Cytochrome P-450 Enzyme System