title: Morphostructural analysis of human follicular stem cells on highly porous bone hydroxyapatite scaffold.
abstract: In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3-6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.
authors.affiliation: Dept. of Stomatology and Oral Science, Division of Oral Surgery, University G. d'Annunzio, Chieti, Ialy. tete@unich.it
authors.forename: S
authors.initials: S
authors.lastname: Tetè
authors.forename: F
authors.initials: F
authors.lastname: Mastrangelo
authors.forename: L
authors.initials: L
authors.lastname: Carone
authors.forename: E
authors.initials: E
authors.lastname: Nargi
authors.forename: G
authors.initials: G
authors.lastname: Costanzo
authors.forename: R
authors.initials: R
authors.lastname: Vinci
authors.forename: F
authors.initials: F
authors.lastname: Burruano
authors.forename: S
authors.initials: S
authors.lastname: Tortorici
authors.forename: V
authors.initials: V
authors.lastname: Dadorante
authors.forename: F
authors.initials: F
authors.lastname: Caciagli
authors.forename: T
authors.initials: T
authors.lastname: Traini
authors.forename: E
authors.initials: E
authors.lastname: Gherlone
authors.forename: A
authors.initials: A
authors.lastname: Caraffa
authors.forename: V
authors.initials: V
authors.lastname: Salini
authors.forename: P
authors.initials: P
authors.lastname: Conti
authors.forename: R
authors.initials: R
authors.lastname: Ciccarelli
mesh_terms: D001672:Biocompatible Materials; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite; D005109:Extracellular Matrix / Q000648:ultrastructure; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D016062:Porosity; D013234:Stem Cells / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014070:Tooth / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite