title: Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay.
abstract: Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a (3)H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients.
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Makito
authors.initials: M
authors.lastname: Tanaka
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yinyan
authors.initials: Y
authors.lastname: Xu
authors.forename: Nao
authors.initials: N
authors.lastname: Yoshida
authors.forename: Ayami
authors.initials: A
authors.lastname: Yoshimi
authors.forename: Itzel Bustos
authors.initials: IB
authors.lastname: Villalobos
authors.forename: Asahito
authors.initials: A
authors.lastname: Hama
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nishio
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Hidaka
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yagasaki
authors.forename: Seiji
authors.initials: S
authors.lastname: Kojima
mesh_terms: D049109:Cell Proliferation; D002675:Child, Preschool; D005260:Female; D011905:Genes, ras; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D054429:Leukemia, Myelomonocytic, Juvenile / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D008297:Male; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000235:genetics; D013936:Thymidine / Q000378:metabolism*
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C516724:PTPN11 protein, human; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D013936:Thymidine