Protective effect of Phyllanthus polyphyllus on acetaminophen induced hepatotoxicity in rats.
Methanolic extract of Phyllanthus-polyphyllus was evaluated for hepatoprotective and antioxidant activities in rats. The plant extract (200 and 300 mg/kg, p.o.) showed a remarkable hepatoprotective and antioxidant activity against acetaminophen induced hepatotoxicity as judged from the serum marker enzymes and antioxidant levels in liver tissues. Acetaminophen induced a significant rise in aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin, gamma glutamate transpeptidase (GGTP), lipid peroxidase (LPO) with a reduction of total protein, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione S-transferase (GST). Treatment of rats with different doses of plant extract (200 and 300 mg/kg) significantly (P<0.001) altered serum marker enzymes and antioxidant levels to near normal against acetaminophen treated rats. The activity of the extract at dose of 300 mg/kg was comparable to the standard drug, silymarin (50 mg/kg, p.o.). Histopathological changes of liver sample were compared with respective control. Results indicate the hepatoprotective and antioxidant properties of Phyllanthus polyphyllus against acetaminophen-induced hepatotoxicity in rats.
B
Rajkapoor
R
Department of Pharmacology, Vels College of Pharmacy, Velan Nagar, Pallavaram, Chennai-600 117, India. rajkapoorb@yahoo.co.in
Y
Venugopal
V
J
Anbu
A
N
Harikrishnan
H
M
Gobinath
G
V
Ravichandran
R
D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D029803:Phyllanthus / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D023382:Toxicity Tests, Acute
D018712:Analgesics, Non-Narcotic; D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D000082:Acetaminophen; D002374:Catalase