Survivin gene RNA interference induces apoptosis in human HL60 leukemia cell lines.
BACKGROUND
It has been demonstrated that survivin, a member of the inhibitor of apoptosis (IAP) protein family, is expressed in human cancers but is undetectable in normal differentiated tissues. HL60 siRNA was introduced into HL60 cells to investigate its effect on cancer cell growth.


METHODS
The opposite livability on HL60 cells was assayed with an MTT test. The change of mRNA and protein of the survivin gene were detected by reverse transcriptase polymerase chain reaction and Western blot, respectively. Cell apoptosis was assayed by flow cytometry.


RESULTS
The growth of HL60 cells decreased by 65.3%, 62.1%, and 52.4% when they transfected with 400 nM siRNA lyh1, lyh2, and lyh3 after 48 hours, in comparison to the control group. Also, the mRNA and protein were knocked down and HL60 cells presented an increase in apoptosis index.


CONCLUSIONS
Small interfering RNA can exert a knockdown of survivin gene expression in HL60 cells, and effectively induce apoptosis and inhibit the growth of leukemia cells.
Lu
Yun-Hua
YH
Research Centre of Biotechnology, China Pharmaceutical University, NanJing, China.
Luo
Xue-Gang
XG
Tao
Xi
X
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D020128:Inhibitory Concentration 50; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D000077022:Survivin; D014162:Transfection
D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D000077022:Survivin; D002945:Cisplatin