title: [Differential proteomic analysis of nasopharyngeal carcinoma cell lines 5-8F and 6-10B].
abstract: OBJECTIVE
  To compare the proteome difference of nasopharyngeal carcinoma (NPC) cell lines 5-8F and 6-10B, and to screen these proteins associated with NPC metastasis.
  
  
  METHODS
  Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background, respectively. PDQuest software was applied to analyze 2-DE images, and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.
  
  
  RESULTS
  2-DE maps of total proteins from 5-8F and 6-10B were established. A total of 65 differential protein spots in the 2 cell lines were found, and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B, which was consistent with the Results from the comparative proteomic analysis.
  
  
  CONCLUSIONS
  Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.
authors.affiliation: Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Xiang
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Peng-fei
authors.initials: PF
authors.lastname: Zhang
authors.forename: Mao-yu
authors.initials: MY
authors.lastname: Li
authors.forename: Zhu-chu
authors.initials: ZC
authors.lastname: Chen
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Xiao
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D013058:Mass Spectrometry; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D020543:Proteome