title: Suprahepatic vena cava manipulative bleeding alleviates hepatic ischemia-reperfusion injury in rats.
abstract: BACKGROUND
  Little is known about time course and peak level of reactive oxygen species in suprahepatic vena cava after liver ischemia-reperfusion.
  
  
  OBJECTIVE
  To determine time course and peak level of reactive oxygen species in suprahepatic vena cava after liver ischemia-reperfusion. To focus on the effects of suprahepatic vena cava manipulative bleeding on the hepatic ischemia-reperfusion injury in rat model.
  
  
  METHODS
  In experiment Part I, blood was taken from suprahepatic vena cava and infrahepatic vena cava for malondialdehyde detection at different time points after reperfusion. Furthermore, we treated the experimental rats in Part II by suprahepatic vena cava manipulative bleeding or infrahepatic vena cava manipulative bleeding at 10 min after reperfusion.
  
  
  RESULTS
  In experiment Part I, malondialdehyde concentration in suprahepatic vena cava elevated obviously with time and peaked at 10 min after reperfusion. The numbers of accumulated polymorphonuclear neutrophils was significantly increased in ischemia-reperfusion group from 10 min after reperfusion, compared with sham-operated group. In Part II, 2% of body weight suprahepatic vena cava manipulative bleeding with blood transfusion at 10 min after reperfusion significantly decreased circulating malondialdehyde, tumour necrosis factor-alpha, endothelin-1, hyaluronic acid, alanine aminotransferase, aspartate aminotransferase levels and polymorphonuclear neutrophil infiltrations in the liver. The 7-day survival rate of this group was 68.75% (11/16) which was significantly higher than other groups.
  
  
  CONCLUSIONS
  We provided the first evidence that 2% of body weight suprahepatic vena cava manipulative bleeding with blood transfusion at 10 min after reperfusion significantly prevented liver ischemia-reperfusion injury.
authors.affiliation: Department of Hepatobiliary Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Hangzhou, PR China.
authors.forename: C
authors.initials: C
authors.lastname: Jia
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000818:Animals; D008099:Liver / Q000098:blood supply*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000097:blood*; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D014684:Venae Cavae / Q000737:chemistry*
chemical_list: D017382:Reactive Oxygen Species