[Effect of Coriolus versicolor polysaccharide B on membrane glycosaminoglycans and cellular glutathione changes in RAW264.7 macrophages exposed to angiotensin II].
OBJECTIVE
To investigate the effect of Coriolus versicolor polysaccharide B (CVP-B) on increased membrane glycosaminoglycans (GAG) expression and intracellular glutathione (GSH) of RAW264.7 macrophages exposed to angiotensin II (Ang II).


METHODS
The plasma membrane of RAW264.7 macrophages exposed to Ang II treatment was isolated by ultracentrifugation, and the membrane GAG expression was analyzed using 1, 9-dimethylmethylene blue (DMMB) spectrophotometric assay for sulfated GAG. The intracellular reduced GSH was determined using fluorophotometry.


RESULTS
The GAG content in the macrophage membranes increased by up to 54% following cell exposure to 1.0 micromol/L Ang II, whereas in presence of 1.0 micromol;/L Ang II, CVP-B at 1, 10, and 50 microg/ml decreased the GAG content by 13%, 43% (P<0.01), and 52% (P<0.01), respectively. The macrophage GSH activity decreased by 69% following incubation with 1.0 micromol;/L Ang II for 24 h, and CVP-B treatment at 1, 10, and 50 microg/ml in presence of 1.0 micromol;/L Ang II resulted in significant increment of GSH activity by 31%(P<0.05), 104% (P<0.01), and 168% (P<0.01), respectively.


CONCLUSIONS
These data provide the first evidence that CVP-B inhibits elevated GAG expression in RAW264.7 macrophage membrane induced by Ang II.
Lou
Ning
N
Intensive Care Unit, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. louning@mail.sysu.edu.cn
Ma
Gang
G
Wang
Dao-feng
DF
Zhu
Zhi-wei
ZW
Su
Quan-guan
QG
Fang
Yi
Y
D000363:Agaricales / Q000737:chemistry*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*
D006025:Glycosaminoglycans; D011134:Polysaccharides; D000804:Angiotensin II; D005978:Glutathione