Bright future of optical assays for ion channel drug discovery.
Ion channels are a key target class for drug discovery. The introduction of new and optimized optical probes, including fluorescent protein-based calcium sensors, luminescent photoproteins, voltage-sensitive probes and ion indicators, allows tackling a wide variety of ion channel targets. To make optical assays more physiologically relevant, tools to control the conformational states of ion channels via manipulation of the membrane potential have to be developed. There is no doubt that progress in optical methods will streamline the ion channel drug discovery process.
Molokanova
Elena
E
Invitrogen, 29851 Willow Creek Road, Eugene, OR 97402, United States.
Savchenko
Alex
A
D015374:Biosensing Techniques / Q000379:methods; D015220:Calcium Channels / Q000502:physiology; D015195:Drug Design; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008564:Membrane Potentials; D036103:Nanotechnology; D055095:Optics and Photonics; D015221:Potassium Channels / Q000502:physiology; D011487:Protein Conformation
D015220:Calcium Channels; D005456:Fluorescent Dyes; D007473:Ion Channels; D015221:Potassium Channels; D049452:Green Fluorescent Proteins