title: Identification of novel chromosomal abnormalities and prognostic cytogenetics markers in intracranial pediatric ependymoma.
abstract: Aim of this study was to search for novel chromosomal imbalances and potential prognostic markers in pediatric ependymoma. Tumor DNA, obtained from 20 children with intracranial ependymoma (World Health Organization WHO grades II and III), was analyzed using metaphase-based comparative genomic hybridization (CGH) and fluorescent in situ hybridization (FISH). The novel copy number aberrations (CNAs) here identified are (i) 4q33-qter loss, (ii) 10q25.2-q26.3 gain, (iii) 3q23-qter losses, (iv) 18q22.2 loss, and (v) 19p13.1-p13.3 gain. The combined presence of 6p22-pter and 13q14.3-qter losses predicted significantly reduced survival. Larger studies are warranted to validate these findings.
authors.affiliation: Laboratory of Oncology, Department of Laboratory and Experimental Medicine, Giannina Gaslini Institute, Genoa, Italy. annalisapezzolo@ospedale-gaslini.ge.it
authors.forename: Annalisa
authors.initials: A
authors.lastname: Pezzolo
authors.forename: Valeria
authors.initials: V
authors.lastname: Capra
authors.forename: Alessandro
authors.initials: A
authors.lastname: Raso
authors.forename: Fabio
authors.initials: F
authors.lastname: Morandi
authors.forename: Federica
authors.initials: F
authors.lastname: Parodi
authors.forename: Claudio
authors.initials: C
authors.lastname: Gambini
authors.forename: Paolo
authors.initials: P
authors.lastname: Nozza
authors.forename: Felice
authors.initials: F
authors.lastname: Giangaspero
authors.forename: Armando
authors.initials: A
authors.lastname: Cama
authors.forename: Vito
authors.initials: V
authors.lastname: Pistoia
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Garrè
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001932:Brain Neoplasms / Q000235:genetics* / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics*; D015331:Cohort Studies; D020732:Cytogenetic Analysis; D004806:Ependymoma / Q000235:genetics* / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D009693:Nucleic Acid Hybridization; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor