title: Ionic liquid-based preparation of cellulose-dendrimer films as solid supports for enzyme immobilization.
abstract: Surface-active cellulose films for covalent attachment of bioactive moieties were achieved by codissolution of cellulose with polyamidoamine (PAMAM) dendrimers in an ionic liquid followed by regeneration of the composite as a film. Different generations of PAMAM were used for the formation of cellulose-dendrimer composites, as well as films with the dendrimer covalently bonded to the cellulose by means of the linker 1,3-phenylene diisocyanate. Surface characterization, thermal stability, and utility for immobilization of laccase were determined. The presence of the dendrimer amino groups was confirmed by detailed characterization of the films' surfaces. These modified films exhibit acceptable thermal stability, comparable to that of other regenerated cellulose films, but the number of active functional groups on the surface is much smaller than the theoretical amount expected. Films made with 1,3-phenylene diisocyanate as linker for covalently bound cellulose and dendrimers exhibit a better performance for immobilization of laccase than those prepared by simple mixing of the cellulose and dendrimer. In general, a linear correspondence between the dendrimer generation within the films and the specific activity of immobilized laccase in such films was not observed.
authors.affiliation: Department of Chemistry, Center for Green Manufacturing, and Alabama Institute for Manufacturing Excellence, The University of Alabama, Tuscaloosa, Alabama 35487, USA.
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Bagheri
authors.forename: Héctor
authors.initials: H
authors.lastname: Rodríguez
authors.forename: Richard P
authors.initials: RP
authors.lastname: Swatloski
authors.forename: Scott K
authors.initials: SK
authors.lastname: Spear
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Daly
authors.forename: Robin D
authors.initials: RD
authors.lastname: Rogers
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D050091:Dendrimers; D004800:Enzymes, Immobilized; D007477:Ions
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; D007477:Ions; D002482:Cellulose