title: Swinging effect of salicylic acid on the accumulation of polyhydroxyalkanoic acid (PHA) in Pseudomonas aeruginosa BM114 synthesizing both MCLandSCL-PHA.
abstract: A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell growth. An addition of 20 mM SA selectively inhibited the accumulation of MCL-PHA in decanoate-grown cells by 83% of the control content in one-step cultivation, where overall PHA accumulation was inhibited by only approximately11%. Typically, the molar monomerunit ratio of the PHA for 25 mM decanoate-grown cells changed from 46:4:25:25 (=[3-hydroxybutyrate]:[3-hydroxycaproate]: [3-hydroxyoctanoate]:[3-hydroxydecanoate]) at 0 mM SA (dry cell wt, 1.97 g/l; PHA content, 48.6 wt%) to 91:1:4:4 at 20 mM SA (dry cell wt, 1.85 g/l; PHA content, 43.2 wt%). Thus, the stimulation of SCL-PHA accumulation was observed. Growth of P. aeruginosa BM114 on undecanoic acid also produced a PHA blend composed of 47.4% P(3HB-co-3- hydroxyvalerate) and 52.6% P(3-hydroxyheptanoate-co-3- hydroxynonanoate-co-3-hydroxyundecanoate). Similar to the case of even-carboxylic acids, SA inhibited the accumulation of only MCL-PHA, but stimulated the accumulation of SCLPHA. For all medium-chain fatty acids tested, SA induced a stimulation of SCL-PHA accumulation in the BM114 strain. SA could thus be used to suppress only the formation of MCL-PHA in Pseudomonas spp. accumulating a blend of SCL-PHA and MCL-PHA.
authors.affiliation: Division of Applied Life Sciences (BK21), Graduate School, Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Jong Kook
authors.initials: JK
authors.lastname: Rho
authors.forename: Mun Hwan
authors.initials: MH
authors.lastname: Choi
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Shim
authors.forename: So-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Myeong Ji
authors.initials: MJ
authors.lastname: Woo
authors.forename: Bong-Sung
authors.initials: BS
authors.lastname: Ko
authors.forename: Ki-Whan
authors.initials: KW
authors.lastname: Chi
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Yoon
mesh_terms: D002210:Caprylates / Q000378:metabolism; D003651:Decanoates / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism*; D020156:Salicylic Acid / Q000494:pharmacology*
chemical_list: D002210:Caprylates; D003651:Decanoates; D005227:Fatty Acids; D054813:Polyhydroxyalkanoates; C008776:pelargonic acid; D020156:Salicylic Acid