title: [Effects of vimentin on invasion and metastasis of prostate cancer cell lines PC-3M-1E8 and PC-3M-2B4].
abstract: OBJECTIVE
  Vimentin, a cytoskeleton protein, is involved in regulating the migration and proliferation of cells. This study was to detect the expression of vimentin in prostate cancer cell lines PC-3M-1E8 and PC-3M-2B4, and evaluate the effects of vimentin on invasion and metastasis of prostate cancer cells.
  
  
  METHODS
  Two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/time of flight mass spectrometry (MALDI TOF-MS) were used to detect the expression of vimentin in prostate cancer cell lines PC-3M-1E8 and PC-3M-2B4. Genetic intervention of vimentin gene and in vitro invasion assay were used to investigate the effects of vimentin on the invasion and metastasis of prostate cancer cells.
  
  
  RESULTS
  The protein level of vimentin was higher in PC-3M-1E8 cells with high metastatic potential than in PC-3M-2B4 cells with low metastatic potential. Eukaryotic vectors expressing antisense-and sense-vimentin were constructed successfully and transfected into PC-3M-1E8 and PC-3M-2B4 cells separately. The number of invasive cells was significantly lower in PC-3M-1E8/vas cells with antisense-vimentin than in control PC-3M-1E8/3.1(-) cells (99.3+/-4.8 vs. 319.4+/-6.5, P<0.01), and significantly higher in PC-3M-2B4/vs cells with sense-vimentin than in control PC-3M-2B4/3.1(+) cells (330.5+/-5.8 vs. 98.6+/-7.5, P<0.01).
  
  
  CONCLUSIONS
  Vimentin is a promising marker for predicting the invasion and metastasis of prostate cancer cells.
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
authors.forename: Jun-Cheng
authors.initials: JC
authors.lastname: Wei
authors.forename: Ming-Fu
authors.initials: MF
authors.lastname: Wu
authors.forename: Yong-Tao
authors.initials: YT
authors.lastname: Zhang
authors.forename: Liang-Ping
authors.initials: LP
authors.lastname: Zhao
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Lu
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
mesh_terms: D045744:Cell Line, Tumor; D016373:DNA, Antisense; D015180:Electrophoresis, Gel, Two-Dimensional; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010766:Phosphorylation; D010957:Plasmids; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D014746:Vimentin / Q000235:genetics / Q000378:metabolism*
chemical_list: D016373:DNA, Antisense; D014746:Vimentin; D016392:Proto-Oncogene Proteins pp60(c-src)