title: Diarrheagenic Escherichia coli and Shigella strains isolated from children in a hospital case-control study in Hanoi, Vietnam.
abstract: This case-control study detected and characterized Shigella and diarrheagenic Escherichia coli (DEC) types among Vietnamese children less than 5 years old. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P < 0.05). We used multiplex PCR and DNA probes to detect enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAggEC), enteropathogenic E. coli (EPEC), attaching and effacing E. coli (A/EEC), verocytotoxin-producing E. coli (VTEC), and enterotoxigenic E. coli (ETEC). The prevalences of DEC in the diarrhea and control groups were 25.7 and 10.5%, respectively. In 62 children with diarrhea, 64 DEC strains included 22 EAggEC (8.8%), 2 EIEC (0.8%), 23 A/EEC (9.2%), 7 EPEC (2.8%), and 10 ETEC strains (4.0%). Among controls, 13 DEC strains included 5 EAggEC strains (4.0%), 7 A/EEC strains (5.6%), and 1 EPEC strain. The characterization of DEC by serotypes, antimicrobial susceptibility patterns, virulence genes, and pulsed-field gel electrophoresis showed the occurrence of many different and highly heterogenic DEC subtypes, but common serotypes were found among ETEC, EIEC and EPEC, respectively. Serotyping was used to distinguish between A/EEC and EPEC. However, A/EEC, EPEC, and EAggEC were isolated at high frequency from both cases and controls. Further in-depth studies are needed to better understand important virulence factors of DEC, especially A/EEC, EPEC, and EAggEC.
authors.affiliation: Department of Microbiology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam. hien.nihe@gmail.com
authors.forename: Bui Thi Thu
authors.initials: BT
authors.lastname: Hien
authors.forename: Flemming
authors.initials: F
authors.lastname: Scheutz
authors.forename: Phung Dac
authors.initials: PD
authors.lastname: Cam
authors.forename: Oralak
authors.initials: O
authors.lastname: Serichantalergs
authors.forename: Tran Thu
authors.initials: TT
authors.lastname: Huong
authors.forename: Tran Minh
authors.initials: TM
authors.lastname: Thu
authors.forename: Anders
authors.initials: A
authors.lastname: Dalsgaard
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016022:Case-Control Studies; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D006760:Hospitalization; D006801:Humans; D007223:Infant; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D012703:Serotyping; D012760:Shigella / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D014744:Vietnam / Q000453:epidemiology; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D037521:Virulence Factors