Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo.
Our purpose is to evaluate intramucosal gastric pH video imaging by 2('),7(')-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied in 10 pigs: five in the control group and five with respiratory acidosis induced through rebreathing. The intramucosal pH of the gastric wall is measured every 5 s and the results demonstrate a good correlation (pearson correlation=0.832) between blood gases pH measurements and pH measured with the video endocopic imaging system. Our results confirm the feasibility of using BCECF fluorescence pH imaging to measure intramucosal pH in vivo.
Rochon
Philippe
P
Lille University Hospital, Pavillon Vancostenobel, 59037 Lille Cedex, France.
Jourdain
Mercé
M
Mangalaboyi
Jacques
J
Fourrier
François
F
Soulié-Bégu
Sylvie
S
Buys
Bruno
B
Dehlin
Guy
G
Lesage
Jean Claude
JC
Chambrin
Marie Christine
MC
Mordon
Serge
S
D000142:Acidosis, Respiratory / Q000097:blood / Q000378:metabolism; D000818:Animals; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000098:blood supply / Q000378:metabolism*; D005773:Gastroscopy / Q000379:methods*; D006439:Hemodynamics; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000097:blood / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D034421:Sus scrofa
D005452:Fluoresceins; D005456:Fluorescent Dyes; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D010100:Oxygen