[Kinetics of intracolloidal iodine within the thyroid of newborn rats. Direct imagery using secondary ion mass spectrometry].
The spatiotemporal distribution of cellular uptake site of radiotoxics is essential data for microdosimetric studies. As early as 1950, the heterogeneity of iodine incorporation within the thyroid has been shown using autoradiography. The objective of this study is to describe the kinetic cellular distribution of newly organified iodine in the thyroid of newborn rats using secondary ion mass microscopy (NanoSIMS50). Ionic images obtained at high mass resolution and with a lateral resolution of about 50 nm show that the early distribution of iodine is heterogeneous from one follicle to another, from one thyrocyte to another inside the same follicle, and that this distribution varies as a function of time. The obtained kinetic profile will allow us to refine the studies concerning the aetiopathology of thyroid cancers of the Chernobyl children.
Elbast
Mouhamad
M
Laboratoire de biophysique, faculté de médecine, université Paris-7-Denis-Diderot, BP 416, 75870 Paris cedex 18, France.
Wu
Ting-Di
TD
Guiraud-Vitaux
Françoise
F
Petiet
Anne
A
Hindié
Elif
E
Champion
Christophe
C
Croisy
Alain
A
Guerquin-Kern
Jean-Luc
JL
Colas-Linhart
Nicole
N
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D003102:Colloids; D007455:Iodine / Q000032:analysis; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics* / Q000633:toxicity; D007700:Kinetics; D051381:Rats; D017208:Rats, Wistar; D018629:Spectrometry, Mass, Secondary Ion; D013961:Thyroid Gland / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
D003102:Colloids; D007457:Iodine Radioisotopes; D007455:Iodine