title: Chronic ACE inhibitor treatment increases angiotensin type 1 receptor binding in vivo in the dog kidney.
abstract: OBJECTIVE
  PET imaging has been recently introduced for investigating the type 1 angiotensin II receptor (AT(1)R) in vivo. The goal of the present study was to investigate the effects of acute and chronic exposure to angiotensin converting enzyme inhibitors (ACEI) on the AT(1)R in the dog kidney.
  
  
  METHODS
  Animals were imaged at baseline, after acute intravenous ACEI treatment and after a chronic 2-week exposure to an oral ACEI. Control animals were imaged at identical time points in the absence of ACEI treatment.
  
  
  RESULTS
  In vivo AT(1)R binding expressed by K (i) was increased in the renal cortex by chronic ACEI treatment (p < 0.05). In vitro measurements of AT(1)R density (B (max)) also revealed significant increases in AT(1)R in isolated glomeruli (p < 0.05). Plasma renin activity was increased, but angiotensin II (Ang II) and the Ang II/Ang I ratio showed a weak correlation with chronic ACEI treatment, consistent with an Ang II escape phenomenon.
  
  
  CONCLUSIONS
  This study reveals, for the first time, that chronic ACEI treatment increases AT(1)R binding in vivo in the dog renal cortex.
authors.affiliation: Department of Radiology and Surgery, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: Tamas G
authors.initials: TG
authors.lastname: Zober
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Fabucci
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Phillip R
authors.initials: PR
authors.lastname: Brown
authors.forename: Esen
authors.initials: E
authors.lastname: Seckin
authors.forename: William B
authors.initials: WB
authors.lastname: Mathews
authors.forename: Kathryn
authors.initials: K
authors.lastname: Sandberg
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szabo
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000008:administration & dosage*; D000818:Animals; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D011877:Radionuclide Imaging; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D044140:Receptor, Angiotensin, Type 1