Sphingosinicella soli sp. nov., isolated from an alkaline soil in Korea.
A Gram-negative, motile, rod-shaped bacterial strain, KSL-125(T), was isolated from an alkaline soil from Kwangchun, Korea, and its taxonomic position was investigated in a polyphasic study. Strain KSL-125(T) grew optimally at 30 degrees C, at pH 7.5-8.0 and in the presence of 0.5% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KSL-125(T) joins the cluster comprising the two Sphingosinicella species at a bootstrap resampling value of 100%. The values for similarity between the 16S rRNA gene sequence of strain KSL-125(T) and those of the type strains of the two Sphingosinicella species were 98.9-99.0%. Strain KSL-125(T) contained Q-10 as the predominant ubiquinone and C(17:1)omega6c, C(16:1)omega7c and/or iso-C(15:0) 2-OH and C(18:1)omega7c as the major fatty acids. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and an unidentified phospholipid. The DNA G+C content was 65.1 mol%. Strain KSL-125(T) was distinguishable from the two recognized Sphingosinicella species on the basis of differential phenotypic properties, DNA-DNA relatedness data and repetitive-sequence-based PCR genomic fingerprinting patterns. The phenotypic, phylogenetic and genetic data showed that strain KSL-125(T) represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella soli sp. nov. is proposed. The type strain is KSL-125(T) (=KCTC 12482(T) =DSM 17328(T)).
Yoon
Jung-Hoon
JH
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribbe.re.kr
Kang
So-Jung
SJ
Lee
Jung-Sook
JS
Nam
Sun-Woo
SW
Kim
Wonyong
W
Oh
Tae-Kwang
TK
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013045:Species Specificity; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil