title: Ligand binding site of tear lipocalin: contribution of a trigonal cluster of charged residues probed by 8-anilino-1-naphthalenesulfonic acid.
abstract: Human tear lipocalin (TL) exhibits diverse functions, most of which are linked to ligand binding. To map the binding site of TL for some amphiphilic ligands, we capitalized on the hydrophobic and hydrophilic properties of 8-anilino-1-naphthalenesulfonic acid (ANS). In single Trp mutants, resonance energy transfer from Trp to ANS indicates that the naphthalene group of ANS is proximate to Leu105 in the cavity. Binding energies of TL to ANS and its analogues reveal contributions from electrostatic interactions. The sulfonate group of ANS interacts strongly with the nonconserved intracavitary residue Lys114 and less with neighboring residues His84 and Glu34. This trigonal cluster of residues may play a role in the ligand recognition site for some negatively charged ligands. Because many drugs possess sulfonate groups, the trigonal cluster-sulfonate interaction can also be exploited as a lipocalin-based drug delivery mechanism. The binding of lauric acid and its analogues shows that fatty acids assume heterogeneous orientations in the cavity of TL. Predominantly, the hydrocarbon tail is buried in the cavity of TL and the carboxyl group is oriented toward the mouth. However, TL can also interact, albeit relatively weakly, with fatty acids oriented in the opposite direction. As the major lipid binding protein of tears, the ability to accommodate fatty acids in two opposing orientations may have functional implications for TL. At the aqueous-lipid interface, fatty acids whose carboxyl groups are positioned toward the aqueous phase are available for interaction with TL that could augment stability of the tear film.
authors.affiliation: Department of Pathology, Jules Stein Eye Institute, UCLA School of Medicine, 100 Stein Plaza, Los Angeles, California 90095, USA.
authors.forename: Oktay K
authors.initials: OK
authors.lastname: Gasymov
authors.forename: Adil R
authors.initials: AR
authors.lastname: Abduragimov
authors.forename: Ben J
authors.initials: BJ
authors.lastname: Glasgow
mesh_terms: D000595:Amino Acid Sequence; D000817:Anilino Naphthalenesulfonates / Q000737:chemistry*; D001665:Binding Sites; D002942:Circular Dichroism; D006801:Humans; D054834:Lipocalins / Q000737:chemistry* / Q000235:genetics; D008239:Lysine / Q000737:chemistry; D017354:Point Mutation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013666:Tears / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry
chemical_list: C515594:8-anilino-1-naphthalenesulfonic acid; D000817:Anilino Naphthalenesulfonates; D054834:Lipocalins; D014364:Tryptophan; D008239:Lysine