Effects of prostaglandin E(1) in the genesis of blood capillaries in the ischemic skeletal muscle of rats: ultrastructural analysis.
Our aim was to study the ultrastructural aspects in the genesis of blood capillaries in the lower limb skeletal muscle of rats submitted to ischemia under the action of intramuscular or endovenous prostaglandin E(1) (PGE(1)). Twelve Wistar-UEM rats were used, randomly distributed into three groups of four animals each, equally redistributed into two subgroups, observed at 7 and 14 days as follows: group only with ischemia was considered as control (I), group with ischemia and intramuscular injection of PGE(1) (IM), and group with ischemia and endovenous injection of PGE(1) (EV). Results were analyzed by transmission electronic microscopy (TEM). TEM analysis revealed evidence of new capillary formation. TEM permitted us to identify morphological structures and phenomena in vascular neoformation that might have occurred through angiogenesis and/or vasculogenesis.
Moreschi
Dorival
D
Maringa State University, Maringa, Parana, Brazil. dorival@moreschi.med.br
Fagundes
Djalma José
DJ
Hernandes
Luzmarina
L
Haapalainen
Edna Freymuller
EF
D000527:Alprostadil / Q000008:administration & dosage*; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage*; D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000503:physiopathology; D004195:Disease Models, Animal; D006614:Hindlimb; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D007511:Ischemia / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000187:drug effects* / Q000503:physiopathology / Q000648:ultrastructure; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D043925:Angiogenesis Inducing Agents; D000527:Alprostadil