Amphritea atlantica gen. nov., sp. nov., a gammaproteobacterium from the Logatchev hydrothermal vent field.
A novel Gram-negative, motile, aerobic rod-shaped bacterium was isolated from a Bathymodiolus sp. specimen collected from the Logatchev hydrothermal vent field at the Mid-Atlantic Ridge. The novel strain, M41(T), was catalase- and oxidase-positive and metabolised various carbohydrates and amino acids. It grew well in marine broth with an optimal growth temperature of 31 degrees C to 34 degrees C (range 4-40 degrees C) and salinity requirement of 3% (range 0.3-9%). The pH range for growth was pH 4.6 to 9.5, with an optimum at pH 8.0. The predominant fatty acids were C(16:1)omega7c, C(16:0) and C(18:1)omega7c. The DNA G+C content of strain M41(T) was 52.2 mol%. The 16S rRNA gene sequence was 94 % similar to that of the type strain of Oceanospirillum beijerinckii, the closest cultivated relative. Other related type strains were Oceanospirillum multiglobuliferum (93% gene sequence similarity), Neptunomonas naphthovorans (92%) and Marinobacterium jannaschii (92%). According to phylogenetic analysis and physiological characteristics, it is suggested that strain M41(T) represents a new genus and novel species for which the name Amphritea atlantica gen. nov., sp. nov. is proposed. The type strain is M41(T) (=DSM 18887(T)=LMG 24143(T)).
Gärtner
Andrea
A
Leibniz-Institut für Meereswissenschaften IFM-GEOMAR, Düsternbrooker Weg 20, D-24105 Kiel, Germany.
Wiese
Jutta
J
Imhoff
Johannes F
JF
D000818:Animals; D001267:Atlantic Ocean; D015373:Bacterial Typing Techniques; D001482:Base Composition; D049872:Bivalvia / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S