Effects of growth and tissue type on the kinetics of 13C and 15N incorporation in a rapidly growing ectotherm.
The use of stable isotopes to investigate animal diets, habitat use, and trophic level requires understanding the rate at which animals incorporate the 13C and 15N from their diets and the factors that determine the magnitude of the difference in isotopic composition between the animal's diet and that of its tissues. We determined the contribution of growth and catabolic turnover to the rate of 13C and 15N incorporation into several tissues that can be sampled non-invasively (skin, scute, whole blood, red blood cells, and plasma solutes) in two age classes of a rapidly growing ectotherm (loggerhead turtles, Caretta caretta). We found significant differences in C and N incorporation rates and isotopic discrimination factors (Delta 13C = delta 13Ctissues - delta 13Cdiet and Delta 15N = delta 15Ntissues - delta 15Ndiet) among tissues and between age classes. Growth explained from 26 to 100% of the total rate of incorporation in hatchling turtles and from 15 to 52% of the total rate of incorporation in juvenile turtles. Because growth contributed significantly to the rate of isotopic incorporation, variation in rates among tissues was lower than reported in previous studies. The contribution of growth can homogenize the rate of isotopic incorporation and limit the application of stable isotopes to identify dietary changes at contrasting time scales and to determine the timing of diet shifts. The isotopic discrimination factor of nitrogen ranged from -0.64 to 1.77 per thousand in the turtles' tissues. These values are lower than the commonly assumed average 3.4 per thousand discrimination factors reported for whole body and muscle isotopic analyses. The increasing reliance on non-invasive and non-destructive sampling in animal isotopic ecology requires that we recognize and understand why different tissues differ in isotopic discrimination factors.
Reich
Kimberly J
KJ
Archie Carr Center for Sea Turtle Research and Department of Zoology, University of Florida, Gainesville, FL 32611, USA. kreich@zoology.ufl.edu
Bjorndal
Karen A
KA
Martínez Del Rio
Carlos
C
D000367:Age Factors; D000818:Animals; D002244:Carbon / Q000378:metabolism*; D002247:Carbon Isotopes; D004032:Diet; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes; D013997:Time Factors; D014018:Tissue Distribution; D014426:Turtles / Q000254:growth & development* / Q000378:metabolism*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D002244:Carbon; D009584:Nitrogen