Antiproliferative, antioxidant, and antimutagenic activities of flavonoid-enriched extracts from (Tunisian) Rhamnus alaternus L.: combination with the phytochemical composition.
A pronounced antiproliferative effect on human leukemia K562 cells was shown with flavonoid-enriched extracts from Rhamnus alaternus roots and leaves, with, respectively, IC(50) values of 165 and 210.73 microg/mL. High DPPH radical-scavenging activity (7.21 and 18.84 microg/mL, respectively) and antioxidative effects using the xanthine oxidase assay (IC(50) values of 83.33 and 103.96 microg/mL, respectively) were detected in the presence of the two tested extracts. Although no mutagenic effect was observed when using the Salmonella typhimurium assay system with TA1535 and TA100 strains, the two tested extracts exhibited a high-level protection toward the direct mutagen, sodium azide-induced response.
Ammar
Rebai Ben
RB
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie, Monastir, Tunisie.
Kilani
Soumaya
S
Bouhlel
Ines
I
Ezzi
Lobna
L
Skandrani
Ines
I
Boubaker
Jihed
J
Sghaier
Mohamed Ben
MB
Naffeti
Aicha
A
Mahmoud
Amor
A
Chekir-Ghedira
Leila
L
Ghedira
Kamel
K
D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D049109:Cell Proliferation / Q000187:drug effects*; D004269:DNA, Bacterial / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D051379:Mice; D009154:Mutation / Q000187:drug effects*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots; D031955:Rhamnus / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D019810:Sodium Azide / Q000494:pharmacology; D013481:Superoxides / Q000737:chemistry; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism
D016587:Antimutagenic Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D004269:DNA, Bacterial; D005419:Flavonoids; D016166:Free Radical Scavengers; D010936:Plant Extracts; D013481:Superoxides; D019810:Sodium Azide; D014969:Xanthine Oxidase