Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.
We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.
Zhang
Xiaodong
X
Department of Genetics and Case Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.
Guo
Chunguang
C
Chen
Yueting
Y
Shulha
Hennady P
HP
Schnetz
Michael P
MP
LaFramboise
Thomas
T
Bartels
Cynthia F
CF
Markowitz
Sanford
S
Weng
Zhiping
Z
Scacheri
Peter C
PC
Wang
Zhenghe
Z
D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods*; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D013194:Staining and Labeling
D000939:Epitopes; D011506:Proteins