Platelet-rich plasma and platelet gel preparation using Plateltex.
BACKGROUND
The platelet gel is made by embedding concentrate platelets within a semisolid (gel) network of polymerized fibrin. It is believed that this blood component will be used more and more in the treatment of several clinical conditions and as an adjunctive material in tissue engineering. Several systems are available to produce platelet-rich plasma (PRP) for topical therapy. Recently, a new system became commercially available, Plateltex. Here we report the technical performance of this system in comparison with the performance of other commercially available systems: PRGF, PRP-Landesber, Curasan, PCCS, Harvest, Vivostat, Regen and Fibrinet.


METHODS
Both the PRP and the gel were prepared according to the manufacturer's directions. The blood samples of 20 donors were used. The yield, the efficiency, and the amount of platelet-derived growth factor AB (PDGF-AB), transforming growth factor beta, vascular endothelial growth factor and fibroblast growth factor were measured in the resulting PRP. The feature of the batroxobin-induced gelation was evaluated.


RESULTS
The yield, the collection efficiency and the growth factor content of Plateltex were comparable to those of most of the other available systems. The gelation time was not dependent on the fibrinogen concentration; however, it was strongly influenced by the contact surface area of the container where the clotting reaction took place (P < 0.0001).


CONCLUSIONS
Plateltex provided platelet recovery, collection efficiency and PDGF-AB availability close to those provided by other systems marketed with the same intended use. Batroxobin, the enzyme provided to induce gelation, acts differently from thrombin, which is used by most other systems. Platelets treated with thrombin become activated; they release their growth factors quickly. Furthermore, thrombin-platelet interaction is a physiological mechanism that hastens the clot-retraction rate. On the contrary, platelets treated with batroxobin do not become activated; they are passively entrapped within the fibrin network, and their growth factor release occurs slowly. In these conditions, the clot retraction takes longer to occur. According to these differences between thrombin and batroxobin, it is expected that batroxobin-induced PRP activation will tailor slow release of the platelet content, thus, providing longer in loco availability of trophic factors. In selected clinical conditions, this durable anabolic factor availability might be preferable to quick thrombin-induced growth factor release.
Mazzucco
L
L
Blood Transfusion Centre and Biotechnology Laboratory, Ospedale SS Antonio e Biagio, Alessandria, Italy.
Balbo
V
V
Blood Transfusion Centre and Biotechnology Laboratory, Ospedale SS Antonio e Biagio, Alessandria, Italy.
Cattana
E
E
Blood Transfusion Centre and Biotechnology Laboratory, Ospedale SS Antonio e Biagio, Alessandria, Italy.
Borzini
P
P
Blood Transfusion Centre and Biotechnology Laboratory, Ospedale SS Antonio e Biagio, Alessandria, Italy.
D012103:Batroxobin; D001709:Biotechnology; D001781:Blood Component Removal / Q000379:methods; D001792:Blood Platelets; D005782:Gels; D006133:Growth Substances / Q000302:isolation & purification; D006801:Humans; D010976:Platelet Count; D017713:Platelet Transfusion; D010982:Platelet-Derived Growth Factor / Q000302:isolation & purification; D053657:Platelet-Rich Plasma; D023822:Tissue Engineering
D005782:Gels; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; C095477:platelet-derived growth factor AB; D012103:Batroxobin