Distribution of Symbiobacterium thermophilum and related bacteria in the marine environment.
We study the ecological distribution of a unique syntrophic bacterium, Symbiobacterium thermophilum, and related bacteria. In this study, we found that they were frequently obtained from seashells and several marine samples. Symbiobacterium also grew from sterilized oyster shells incubated undersea for 2 or 3 months on the coast of Shimoda, Shizuoka, Japan. 16S rRNA gene-based phylogeny of the clones obtained from the Symbiobacterium-positive cultures demonstrated the potential diversity of this bacterial group, which constitutes a distinct clade between Actinobacteria and Firmicutes. We successfully isolated two new Symbiobacterium strains from oyster shells. 16S rRNA gene-based phylogeny indicated that one belongs to S. thermophilum, and that the other is affiliated with a different species. We also isolated Ureibacillius spp., which showed activity supporting the growth of S. thermophilum.
Sugihara
Takafumi
T
Life Science Research Center, College of Bioresource Sciences, Nihon University, Kameino, Fujisawa,Japan.
Watsuji
Tomo-o
TO
Kubota
Shin
S
Yamada
Kazune
K
Oka
Kaori
K
Watanabe
Kiyoshi
K
Meguro
Michiko
M
Sawada
Emi
E
Yoshihara
Kiyoshi
K
Ueda
Kenji
K
Beppu
Teruhiko
T
D039903:Actinobacteria / Q000145:classification; D000818:Animals; D017931:DNA Primers; D005243:Feces / Q000382:microbiology; D005843:Geography; D007564:Japan; D007777:Lactobacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010125:Ostreidae / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D012624:Seaweed / Q000382:microbiology; D014871:Water Microbiology
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S