Molecular typing of Vibrio parahaemolyticus isolated from seafood harvested along the south-west coast of India.
OBJECTIVE
Evaluation of protein profiling for typing Vibrio parahaemolyticus using 71 strains isolated from different seafood and comparison with other molecular typing techniques such as random amplified polymorphic DNA analysis (RAPD) and enterobacterial repetitive intergenic consensus sequence (ERIC)-PCR.


RESULTS
Three molecular typing methods were used for the typing of 71 V. parahaemolyticus isolates from seafood. RAPD had a discriminatory index (DI) of 0.95, while ERIC-PCR showed a DI of 0.94. Though protein profiling had less discriminatory power, use of this method can be helpful in identifying new proteins which might have a role in establishment in the host or virulence of the organism.


CONCLUSIONS
The use of protein profiling in combination with other established typing methods such as RAPD and ERIC-PCR generates useful information in the case of V. parahaemolyticus associated with seafood.


CONCLUSIONS
The study demonstrates the usefulness of nucleic acid and protein-based studies in understanding the relationship between various isolates from seafood.
Bhowmick
P P
PP
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences, University, College of Fisheries, Mangalore, India.
Khushiramani
R
R
Raghunath
P
P
Karunasagar
I
I
Karunasagar
I
I
D001426:Bacterial Proteins / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007194:India; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D017747:Seafood / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; D004269:DNA, Bacterial