title: In vitro antioxidant, antimutagenic and genoprotective activity of Rosa roxburghii fruit extract.
abstract: The antioxidant properties of the fruit of the Rosa roxburghii (RR) plant have been associated with several putative health promoting effects. The possible cytotoxic, mutagenic/antimutagenic and genotoxic effects of RR fruit extract were investigated. The effect on antioxidant status and protection against induced oxidative stress were also investigated using primary rat hepatocytes. A RR fruit extract containing 45 g/L total ascorbic acid and 65 g/L total polyphenols was used in this study. Dilutions up to 0.08% (v/v) increased significantly the antioxidant status in primary rat hepatocytes. The glutathione redox state was decreased with RR treatment but was increased in Chang liver cells and MT-2 lymphoblast. No cyto- or genotoxicity were observed at levels of up to 5% (v/v) of the fruit extract. In addition, a significant protection against t-BHP induced oxidative stress was observed in primary rat hepatocytes. The Ames test revealed no mutagenic activity using the Salmonella typhimurium strains TA98, TA100 and TA102. A significant antimutagenic effect of the extract was observed against the metabolic activated mutagens 2-acetylaminofluorene and aflatoxin B1 and to a lesser extent against methyl methanesulfonate. It is concluded that these results support the associated health promoting potential of Rosa Roxburghii fruit and in particular against oxidative stress.
authors.affiliation: School for Biochemistry, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa. Francois.vanderWesthuizen@nwu.ac.za
authors.forename: Francois H
authors.initials: FH
authors.lastname: van der Westhuizen
authors.forename: Catharina S Janse
authors.initials: CS
authors.lastname: van Rensburg
authors.forename: George S
authors.initials: GS
authors.lastname: Rautenbach
authors.forename: Jeanine L
authors.initials: JL
authors.lastname: Marnewick
authors.forename: Du Toit
authors.initials: du T
authors.lastname: Loots
authors.forename: Cristal
authors.initials: C
authors.lastname: Huysamen
authors.forename: Roan
authors.initials: R
authors.lastname: Louw
authors.forename: Pieter J
authors.initials: PJ
authors.lastname: Pretorius
authors.forename: Elardus
authors.initials: E
authors.lastname: Erasmus
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005978:Glutathione / Q000032:analysis / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D020122:tert-Butylhydroperoxide; D005978:Glutathione