title: Perfusion preservation versus static preservation for cardiac transplantation: effects on myocardial function and metabolism.
abstract: BACKGROUND
  Continuous perfusion of donor hearts for transplantation has received increasing interest, but the effects on cellular metabolism, myocyte necrosis, and myocardial edema are not well defined.
  
  
  METHODS
  Pig hearts were instrumented with sonomicrometry crystals and left ventricular catheters. Left ventricular function was quantified by the pre-load-recruitable stroke work (PRSW) relationship. Hearts were arrested with Celsior solution with 5.5 mM 13C-glucose added, and removed and stored in cold solution (n = 4) or placed in a device providing continuous perfusion of this solution at 10 ml/100 g/min (n = 4). After 4 hours of storage, left atrial samples were frozen, extracted, and analyzed by magnetic resonance spectroscopy. Hearts were then transplanted into recipient pigs and reperfused for 6 hours, with function measured hourly. At the end of the experiment, left ventricular water content and serum creatine kinase-MB isoenzyme levels were measured.
  
  
  RESULTS
  Baseline left ventricular function was similar in both groups. During reperfusion, the volume-axis intercept of the PRSW relationship was significantly lower in hearts stored with continuous perfusion (p < 0.05), suggesting reduced contractile impairment. Magnetic resonance spectroscopy revealed a decrease in tissue lactate in hearts that received continuous perfusion. Serum creatine kinase-MB isoenzyme levels were higher hearts that had static storage (30.8 +/- 9.0 vs 13.2 +/- 2.7 ng/ml; p < 0.05). Left ventricular water content was similar in both groups (0.797 +/- 0.012 vs 0.796 +/- 0.014; p = 0.45).
  
  
  CONCLUSIONS
  Donor hearts sustain less functional impairment after storage with continuous perfusion. This technique reduces tissue lactate accumulation and myocardial necrosis without increasing myocardial edema and appears promising as a method to improve results of cardiac transplantation.
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8879, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Rosenbaum
authors.forename: Matthias
authors.initials: M
authors.lastname: Peltz
authors.forename: J Michael
authors.initials: JM
authors.lastname: DiMaio
authors.forename: Dan M
authors.initials: DM
authors.lastname: Meyer
authors.forename: Michael A
authors.initials: MA
authors.lastname: Wait
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Merritt
authors.forename: W Steves
authors.initials: WS
authors.lastname: Ring
authors.forename: Michael E
authors.initials: ME
authors.lastname: Jessen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016027:Heart Transplantation / Q000379:methods*; D007036:Hypothermia, Induced / Q000379:methods*; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010477:Perfusion / Q000379:methods*; D013552:Swine; D016276:Ventricular Function / Q000502:physiology*
chemical_list: D019188:Organ Preservation Solutions; D019344:Lactic Acid