title: Expression of secretory leukocyte proteinase inhibitor in the submandibular glands of AIDS patients.
abstract: OBJECTIVE
  Secretory leukocyte proteinase inhibitor (SLPI) is an endogenous proteinase inhibitor present in mucosal secretions. It also displays antimicrobial activity including anti-human immunodeficiency virus activity. This protease inhibitor is also expressed in submandibular glands (SMG), but there are few data on its expression in AIDS patients with infectious conditions.
  
  
  METHODS
  We analyzed the expression of SLPI using immunohistochemistry in submandibular gland samples of 36 AIDS patients [10 with normal histology, 10 with chronic nonspecific sialadenitis, eight with mycobacteriosis, and eight with cytomegalovirus (CMV) infection] and 10 HIV-negative controls. The proteinase inhibitor was quantified using image analysis and expressed as % of positively stained area.
  
  
  RESULTS
  There was a higher expression of SLPI in AIDS patients with CMV infection (% of stained area, mean+/-SD: 37.37+/-14.45) when compared with all other groups (P=0.009). There were no significant differences between control subjects (22.70+/-9.42%) and AIDS patients without histologic alterations (18.10+/-7.58%), with chronic nonspecific sialadenitis (17.13+/-5.36%), or mycobacterial infection (21.09+/-4.66%).
  
  
  CONCLUSIONS
  Cytomegalovirus infection increases SLPI expression in the SMG of AIDS patients. Our results reveal new insights into the pathogenic association between HIV and CMV in AIDS patients.
authors.affiliation: Department of Oral Pathology, Faculty of Odontology of Piracicaba- University of Campinas, Piracicaba, SP, Brazil.
authors.forename: L A
authors.initials: LA
authors.lastname: Rocha
authors.forename: P A
authors.initials: PA
authors.lastname: Vargas
authors.forename: L F F
authors.initials: LF
authors.lastname: Silva
authors.forename: J E
authors.initials: JE
authors.lastname: Leon
authors.forename: A B
authors.initials: AB
authors.lastname: Santos
authors.forename: P S
authors.initials: PS
authors.lastname: Hiemstra
authors.forename: T
authors.initials: T
authors.lastname: Mauad
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000473:pathology; D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology*; D000328:Adult; D000368:Aged; D002423:Cause of Death; D002908:Chronic Disease; D003586:Cytomegalovirus Infections / Q000473:pathology; D005260:Female; D018023:HIV Seronegativity; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009164:Mycobacterium Infections / Q000473:pathology; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000032:analysis*; D012793:Sialadenitis / Q000473:pathology; D013363:Submandibular Gland / Q000473:pathology*; D013364:Submandibular Gland Diseases / Q000473:pathology
chemical_list: D053481:Secretory Leukocyte Peptidase Inhibitor