[Determination of tetrandrine and fangchinoline in Radix Stephaniae tetrandrae and its preparation by nonaqueous capillary chromatography].
OBJECTIVE
To establish a new method for the determination of fangchinoline and tetrandrine in Stephania tetrandra and Fengtongan capsule by noanqueous capillary electrophoresis.


METHODS
Separation was carried out in an uncoated fused capillary (50 cm x 75 microm i.d.) with a running buffer containing 50 mmol x L(-1) ammonium acetate, 1.0% acetic acid and 20% acetonitrile in methanol. A separation voltage of 20 kV and a UV detector wavelength at 214 nm were adopted. Sample was introduced from the anode.


RESULTS
The calibration ranges were 1.00, 500 mg x L(-1) for both analytes. Under the optimum conditions, the relative standard deviation (RSD, n = 6) for the migration time of each analyte were 0.09%, 1.9% (intra-day) and 0.63%, 1.9% (inter-day); The RSD for the peak area of each analyte were 0.45%, 5.9% (intra-day) and 2.3%, 5.6% (inter-day), respectively. The contents of the analytes were determined easily with average recoveries 102% for fangchinoline and 105% for tetrandrine in S. tetrandra and 94.6% for fangchinoline and 98.7% for tetrandrine in Fengtongan capsules, respectively.


CONCLUSIONS
The proposed method is simple, rapid, accurate and higher repeatable, and can be used to control of the quality of S. tetrandra and Fengtongan capsules.
Li
Yu-Qin
YQ
College of Pharmacy, Taishan Medicine College, Taian 271016, China. lqq29@163.com
Chen
Xing-Guo
XG
Qi
Yong-Xiu
YX
Li
Ke
K
Jia
Bao-Xiu
BX
Liu
Cai-Hong
CH
Cao
Ming-Liang
ML
D044182:Benzylisoquinolines / Q000032:analysis*; D002138:Calibration; D053801:Capillary Electrochromatography / Q000379:methods*; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D031609:Stephania tetrandra / Q000737:chemistry*
D044182:Benzylisoquinolines; D002214:Capsules; D004365:Drugs, Chinese Herbal; C009438:tetrandrine; C060802:fangchinoline