Differential interleukin-10 production stratified by -571 promoter polymorphism in purified human immune cells.
A C-to-A base substitution has been identified at bp -571 in the IL-10 promoter and has been linked to numerous diseases. To investigate the role of this polymorphism on IL-10 production, T cells, B cells and monocytes were enriched from peripheral blood from subjects either homozygous for the C or A allele. Treatment of monocytes and B cells with lipopolysaccharide from individuals homozygous for the C allele resulted in higher levels of IL-10 production as compared to monocytes from individuals homozygous for the A allele. Though not statistically significant, when B cells were treated with anti-IgM or T cells with concanavalin A higher levels of IL-10 were produced from individuals homozygous for the A allele. Changes in IL-10 protein production were paralleled by similar changes in IL-10 mRNA production. These results demonstrate that changes in IL-10 production observed due to the -571 genotype depend on both cell type and stimulus.
Steinke
John W
JW
Asthma and Allergic Diseases Center, Beirne Carter Center for Immunology Research, University of Virginia, 409 Lane Road, Room 5031, P.O. Box 801355, Charlottesville, VA 22908-1355, USA. js3ch@virginia.edu
Barekzi
Elizabeth
E
Huyett
Phillip
P
Borish
Larry
L
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D018189:Immunomagnetic Separation; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics*; D008213:Lymphocyte Activation; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D012333:RNA, Messenger; D016753:Interleukin-10