title: Comparative proteomics analysis of light responses in cryptochrome1-304 and Columbia wild-type 4 of Arabidopsis thaliana.
abstract: The blue light photoreceptor mutant cryptochrome1-304 (cry1-304) and Columbia wild-type 4 (col-4) of Arabidopsis thaliana were grown under white light and blue light, and in the dark. To study the difference in protein expression levels between cry1-304 and col-4, a proteomic approach was applied based on 2-D gel electrophoresis. Twenty-one different protein spots were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. The expression of four genes corresponding to four protein spots was analyzed by semiquantitative reverse transcription-polymerase chain reaction. We applied analytical procedures to study cry1-304 and col-4, and found that the differentially expressed proteins formed six clusters reflecting co-regulation. This assessment was consistent with the known physiological responses of plants to light.
authors.affiliation: College of Life Science and Biotechnology, Hunan University, Changsha 410082, China.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Xinhong
authors.initials: X
authors.lastname: Guo
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Xiao
authors.forename: Dongying
authors.initials: D
authors.lastname: Tang
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000502:physiology* / Q000528:radiation effects*; D029681:Arabidopsis Proteins; D056931:Cryptochromes; D005420:Flavoproteins / Q000378:metabolism*; D008027:Light; D005810:Multigene Family / Q000502:physiology*; D018462:Photobiology / Q000379:methods; D020543:Proteome / Q000378:metabolism*; D011829:Radiation Dosage
chemical_list: D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D056931:Cryptochromes; D005420:Flavoproteins; D020543:Proteome