Human umbilical cord blood-derived CD34+ cells can be used as a prophylactic agent for experimental heatstroke.
We attempted to assess the prophylactic effect of human umbilical cord blood-derived CD34(+) cells in experimental heatstroke. Anesthetized rats, 1 day before heat stress, were divided into 2 major groups and given CD34(-) cells (defined by 1 x 10(6) human cord blood lymphocytes and monocytes that contained <0.2% CD34(+) cells) or CD34(+) cells (defined by 1 x 10(6) human cord blood lymphocytes and monocytes that contained >95% CD34(+) cells). They were exposed to ambient temperature of 43 degrees C for 70 min to induce heatstroke. When the CD34(-) cells-treated or untreated rats underwent heat stress, their survival time values were found to be 20-24 min. Pretreatment with CD34(+) cells significantly increased survival time (123-351 min). As compared with normothermic controls, all CD34(-) cells-treated heatstroke animals displayed hypotension, hepatic and renal failure, hypercoagulable state, activated inflammation, and cerebral ischemia and injury. However, these heatstroke reactions all were significantly suppressed by CD34(+) cells pretreatment. In addition, the levels of interleukin-10 in plasma and glial cell line-derived neurotrophic factors in brain were all significantly increased after CD34(+) cell administration during heatstroke. Our data indicate that human umbilical cord-derived CD34(+) cells can be used as a prophylactic agent for experimental heatstroke.
Hwang
Wei-Shou
WS
Department of Hematology and Oncology, Chi Mei Medical Center, Tainan, Taiwan.
Chen
Sheng-Hsien
SH
Lin
Cheng-Hsien
CH
Chang
Hsiu-Kang
HK
Chen
Wei-Chun
WC
Lin
Mao-Tsun
MT
D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis*; D001479:Basal Ganglia / Q000378:metabolism; D002561:Cerebrovascular Disorders / Q000209:etiology / Q000517:prevention & control; D036101:Cord Blood Stem Cell Transplantation; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D018883:Heat Stroke / Q000150:complications / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D007674:Kidney Diseases / Q000209:etiology / Q000517:prevention & control; D008107:Liver Diseases / Q000209:etiology / Q000517:prevention & control; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D009410:Nerve Degeneration / Q000209:etiology / Q000517:prevention & control; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D018952:Antigens, CD34; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009566:Nitrates; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10