Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis.
A number of methods have been developed to identify the cells that undergo apoptosis by analyzing the morphological, biochemical, and molecular changes that take place during this universal biological process. The best recognized biochemical hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fluorochrome substrates, as well as employing the fluorescein-labeled CaspaTag pan-caspase in situ detection kit.
Mazumder
Suparna
S
Department of Cancer Biology, Lerner Research Institute, The Cleveland Clinic, Cleveland, OH, USA.
Plesca
Dragos
D
Almasan
Alexandru
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D014408:Biomarkers, Tumor / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods; D004249:DNA Damage / Q000502:physiology*; D004789:Enzyme Activation / Q000502:physiology; D019793:Fluorescein / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods
D014408:Biomarkers, Tumor; D053148:Caspase 3; D019793:Fluorescein