Role of the extracellular domain of Fc epsilon RI alpha in intracellular processing and surface expression of the high affinity receptor for IgE Fc epsilon RI.
The high affinity receptor for immunoglobulin E, Fc epsilon RI, is a critical component of IgE-mediated allergic reactions. It is expressed as a tetramer (alphabetagamma(2)) made of an IgE-binding alpha chain and a signaling module formed by the beta chain and a dimer of gamma chains. It is expressed in humans and rodents on basophils and mast cells at a high level, and, upon activation, it induces the liberation of allergy mediators. In humans a trimeric form lacking the beta chain also exists (alphagamma(2)). This trimeric form is expressed on antigen presenting cells where it acts to facilitate antigen presentation via IgE. Both the expression and the signaling capacity of the trimer are lower than those of the tetramer. The differences between human (tetrameric and trimeric) and murine (tetrameric only) expression is explained in part by the fact that mouse alpha cannot be expressed at the cell surface in the absence of beta, while human alpha can. Here we demonstrate that the capacity of human alpha to be expressed at the cell surface in the absence of beta is encoded entirely in its extracellular domain. These findings show that the extracellular domain of the type I transmembrane protein Fc epsilon RI alpha plays a role in Fc epsilon RI intracellular processing and expression at the cell surface.
Hartman
Mor-Li
ML
Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02115, USA.
Lin
Shih-Yao
SY
Jouvin
Marie-Hélène
MH
Kinet
Jean-Pierre
JP
D000818:Animals; D002999:Clone Cells; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D042541:Intracellular Space / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D017455:Receptors, IgE / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014162:Transfection
D017455:Receptors, IgE; D007073:Immunoglobulin E