Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) genes in Corynebacterium glutamicum R.
The phosphoenolpyruvate : carbohydrate phosphotransferase system (PTS) catalyses the transport of carbohydrates by coupling carbohydrate translocation and phosphorylation. In Corynebacterium glutamicum R, the genes ptsH and ptsI encode general components of the PTS, and genes ptsF, ptsS and ptsG each encode fructose-, sucrose- and glucose-specific components of the PTS, respectively. In this study, we examined the mRNA levels of the pts genes in the presence or absence of PTS sugars. Glucose elevated the expression of ptsG, ptsH and ptsI genes, whereas fructose and sucrose induced the expression of all the pts genes examined, i.e. ptsF, -S, -G, -H and -I. We determined the transcriptional start sites of the pts genes and found that these promoters were activated in the presence of fructose. Disruption of fruR, which is a deoxyribonucleoside repressor (DeoR)-type transcriptional regulator co-transcribed with ptsF, resulted in enhanced induction of the fructose-pts operon, ptsI, and ptsH expression in response to fructose, indicating that FruR attenuates the induction of ptsI, ptsH and fructose-pts by fructose.
Tanaka
Yuya
Y
Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
Okai
Naoko
N
Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
Teramoto
Haruhiko
H
Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
Inui
Masayuki
M
Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
Yukawa
Hideaki
H
Research Institute of Innovative Technology for the Earth (RITE), 9-2, Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D015152:Blotting, Northern; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000502:physiology*; D005632:Fructose / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013395:Sucrose / Q000378:metabolism; D024363:Transcription Initiation Site; D056968:Lac Repressors
D001426:Bacterial Proteins; D005632:Fructose; D005947:Glucose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012097:Repressor Proteins; D013395:Sucrose; C068837:FruR protein, Bacteria; D056968:Lac Repressors