title: Severe antibody-mediated agranulocytosis.
abstract: A 56-year-old female with Crohn's disease was admitted to the hospital with malaise, fever, and a low white blood cell count (0.8 x 10(9)/l) with no granulocytes or myeloid precursor cells in the bone marrow. The leucopenia was initially thought to be the result of an infection and she was treated with antibiotics and granulocyte colony-stimulating factor (G-CSF, filgrastim). However, the bacterial cultures and viral tests were all negative. The patient's condition deteriorated and she became morbidly ill, but recovered after high dose steroid treatment. Six weeks later she relapsed whilst receiving 7.5 mg daily dose of prednisolone. She recovered quickly after being given high dose methylprednisolone in combination with filgrastim. A high maintenance dose of prednisolone was tapered over 5 months. She has not relapsed since and is currently well. Antibodies to the human neutrophil antigen (HNA)-3a were detected, but these antibodies could not easily explain her agranulocytosis as she had a HNA-3a negative phenotype. It seems plausible that her agranulocytosis was immune mediated through autoantibodies directed towards the early myeloid cells.
authors.affiliation: Department of Medicine, University Hospital of North Norway, Tromsø, Norway.
authors.forename: H
authors.initials: H
authors.lastname: Johnsen
authors.forename: A
authors.initials: A
authors.lastname: Husebekk
authors.forename: T
authors.initials: T
authors.lastname: Skjelbakken
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000380:Agranulocytosis / Q000150:complications / Q000276:immunology*; D001323:Autoantibodies / Q000009:adverse effects* / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000627:therapeutic use; D003424:Crohn Disease / Q000150:complications*; D005260:Female; D000069585:Filgrastim; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D006801:Humans; D008875:Middle Aged; D011994:Recombinant Proteins
chemical_list: D000305:Adrenal Cortex Hormones; D001323:Autoantibodies; D003115:Colony-Stimulating Factors; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000069585:Filgrastim