Detection and quantification of sucrose as dietary biomarker using gas chromatography and liquid chromatography with mass spectrometry.
Several epidemiological studies suggest a link between the intake of refined sugars and an increased risk for colorectal, breast, pancreatic and endometrial cancer. However, other studies failed to confirm these conclusions and the reason for this may be the ambiguity of dietary assessment methods - mainly self-reporting - employed. Sucrose is an established biomarker for sugars intake, allowing the objective assessment of dietary sucrose. So far, urinary excretion of sucrose was mainly determined using an enzyme assay. However, this method is time-consuming and labour-intensive. In this study, we present a mass spectrometric method for the determination of sucrose in urine using liquid chromatography with mass spectrometry (LC/MS) which can be used for large-scale epidemiological studies.
Kuhnle
Gunter G C
GG
MRC Dunn Human Nutrition Unit, Cambridge CB2 0XY, UK.
Joosen
Annemiek M C P
AM
Wood
Tommy R
TR
Runswick
Shirley A
SA
Griffin
Julian L
JL
Bingham
Sheila A
SA
D015415:Biomarkers / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004040:Dietary Carbohydrates / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000652:urine*; D016482:Urinalysis / Q000379:methods*
D015415:Biomarkers; D004040:Dietary Carbohydrates; D013395:Sucrose