title: Influenza A virus derived from persistently virus-infected cells shows attenuated cytotoxicity in cultured cells but virulent pathogenicity in mice.
abstract: The IVpi-43 strain of influenza A virus, a progeny virus derived from persistently virus-infected Madin-Darby canine kidney (MDCK) cells, showed a more attenuated nature in cytopathology in cultured cells than the parental wild-type influenza virus (IVwt) that was used for establishment of the virus carrier culture. Upon infection of MDCK cells, growth of the IVpi-43 virus was restrained with an impaired synthesis of virus structural proteins in the cells. Apoptosis induced by IVpi-43 virus was confined at a low level. The IVpi-43 virus was able to easily cause persistent infection in fresh MDCK cells. In contrast to the in vitro phenotype, the IVpi-43 virus proved highly virulent in mice, with massive and broadly disseminated virus multiplication in the lungs. It was suggested that impaired activity of the neuraminidase molecule of the IVpi-43 virus was responsible for the delayed and faint appearance of apoptosis in the IVpi-43 virus-infected respiratory cells, which made it possible for the virus to replicate for a longer period and to spread to a broader area of the lungs and that abundant numbers of the virus-infected lung cells were killed within a short period by the subsequently established virus-specific immune responses, leading to unrecoverable serious pneumonia.
authors.affiliation: Department of Microbiology, Fukui University School of Medicine, Fukui 910-1193, Japan.
authors.forename: Md
authors.initials: M
authors.lastname: Jaber Hossain
authors.forename: Isamu
authors.initials: I
authors.lastname: Mori
authors.forename: Beixing
authors.initials: B
authors.lastname: Liu
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line / Q000821:virology*; D002470:Cell Survival; D004285:Dogs; D009980:Influenza A virus / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity*; D008168:Lung / Q000821:virology; D008297:Male; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism; D009976:Orthomyxoviridae Infections / Q000821:virology*; D016019:Survival Analysis; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000096:biosynthesis; D014774:Virulence; D014779:Virus Replication
chemical_list: D015678:Viral Structural Proteins; D009439:Neuraminidase