title: Prostaglandin F(2alpha) regulates cytokine responses of mast cells through the receptors for prostaglandin E.
abstract: There is an increasing body of evidence that prostanoids modulate mast cell functions and contribute to the development of allergic inflammation. The present study aimed to identify an undetermined function of prostaglandin (PG) F(2alpha) in mast cell activation and the signaling mechanism involved in it. Simultaneous quantification of prostanoids by liquid chromatography/tandem mass spectrometry revealed the constitutive release of PGF(2alpha), thromboxane B(2), and 6-keto-PGF(1alpha) from bone marrow-derived mast cells (BMMCs). Upon activation of BMMCs by lipopolysaccharide, the cytokine production in BMMCs was enhanced when the culture was supplemented with PGF(2alpha). However, F prostanoid receptor-a selective receptor for PGF(2alpha)-was not detected in BMMCs. Further investigations performed using prostanoid receptor antagonists revealed an alternative mechanism wherein the receptors for PGE species-E prostanoid receptors-mediated the PGF(2alpha) signal in BMMCs. The present study provides an insight into a novel function of PGF(2alpha), i.e., an autocrine accelerator for mast cell activation.
authors.affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, 2-1 Seiryo, Aoba-ku, Sendai, Miyagi 980-8575, Japan.
authors.forename: Izumi
authors.initials: I
authors.lastname: Kaneko
authors.forename: Takanori
authors.initials: T
authors.lastname: Hishinuma
authors.forename: Kaori
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Owada
authors.forename: Noriko
authors.initials: N
authors.lastname: Kitanaka
authors.forename: Hisatake
authors.initials: H
authors.lastname: Kondo
authors.forename: Junichi
authors.initials: J
authors.lastname: Goto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Furukawa
authors.forename: Masao
authors.initials: M
authors.lastname: Ono
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D019898:Autocrine Communication / Q000187:drug effects; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D015237:Dinoprost / Q000494:pharmacology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011453:Prostaglandins / Q000378:metabolism; D018078:Receptors, Prostaglandin E / Q000187:drug effects / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013929:Thromboxane B2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D008070:Lipopolysaccharides; D011453:Prostaglandins; D018078:Receptors, Prostaglandin E; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost