Occurrence and species level diagnostics of Campylobacter spp., enteric Helicobacter spp. and Anaerobiospirillum spp. in healthy and diarrheic dogs and cats.
In order to study the occurrence and co-infection of different species of Campylobacter, enteric Helicobacter and Anaerobiospirillum in dogs and cats and define a possible association between these microrganisms and gastrointestinal disorders, 190 dogs and 84 cats, either healthy or with diarrhea, were sampled between 2002 and 2003. Thirty-three C. upsaliensis, 17 C. jejuni, 2 C. helveticus, 1 C. lari isolates from dogs and 14 C. helveticus, 7 C. jejuni, 6 C. upsaliensis isolates from cats were identified using species-specific PCR and phenotypic tests. Whole cell protein profile analysis, phenotypic tests, PCR-RFLP of gyrB and a phylogenetic study of partial groEL and 16S rRNA sequences were used to identify 37 H. bilis, 22 H. canis and 14 H. cinaedi in dogs and 12 H. canis, 5 H. bilis and 2 H. cinaedi in cats. Whole cell protein profile analysis, phenotypic tests and species-specific PCR of 16S rRNA were used to identify 14 A. succiniciproducens, 12 A. thomasii isolates and one unidentified Anaerobiospirillum sp. isolate in dogs and 3 A. thomasii isolates in cats. Fifty-two animals (19%) were positive for the isolation of more than one genus. No significant statistical correlation was found between any isolates of Campylobacter, Helicobacter or Anaerobiospirillum spp. or the various co-infection rates, and the presence of diarrhea in either dogs or cats. Campylobacter isolates were also tested for antibiotic resistance using the agar dilution method.
Rossi
M
M
Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, Ozzano Emilia, Bologna, Italy. mirko.rossi4@unibo.it
Hänninen
M L
ML
Revez
J
J
Hannula
M
M
Zanoni
R G
RG
D044064:Anaerobiospirillum / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002167:Campylobacter / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D002169:Campylobacter Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D002415:Cats / Q000382:microbiology*; D003967:Diarrhea / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D016998:Helicobacter / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S