Development of a high analytical performance amperometric glucose biosensor based on glucose oxidase immobilized in a composite matrix: layered double hydroxides/chitosan.
This paper aimed at showing the interest of the composite material based on layered double hydroxides (LDHs) and chitosan (CHT) as suitable host matrix likely to immobilize enzyme onto electrode surface for amperometric biosensing application. This hybrid material combined the advantages of inorganic LDHs and organic biopolymer, CHT. Glucose oxidase (GOD) immobilized in the composite material maintained its activity well as the usage of glutaraldehyde was avoided. The process parameters for the fabrication of the enzyme electrode and various experimental variables such as pH, applied potential and temperature, were explored for optimum analytical performance of the enzyme electrode. The enzyme electrode provided a linear response to glucose over a concentration range of 1 x 10(-6) to 3 x 10(-3) M with a high sensitivity of 62.6 mA M(-1) cm(-2) and a detection limit of 0.1 microM based on the signal-to-noise ratio of 3.
Shi
Qiaofang
Q
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China.
Han
En
E
Shan
Dan
D
Yao
Wenjuan
W
Xue
Huaiguo
H
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004573:Electrolytes; D004798:Enzymes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011108:Polymers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
D004573:Electrolytes; D004798:Enzymes; D006878:Hydroxides; D011108:Polymers; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose