title: The putative peroxisomal gene Pxt1 is exclusively expressed in the testis.
abstract: Genes reported to be crucial for spermatogenesis are often exclusively expressed in the testis. We have identified a novel male germ cell-specific expressed gene named peroxisomal testis specific 1 (Pxt1) with expression starting at the spermatocyte stage during mouse spermatogenesis. The putative amino acid sequence encoded by the cDNA of the Pxt1 gene contains a conserved Asn-His-Leu (NHL)-motif at its C-terminal end, which is characteristic for peroxisomal proteins. Pxt1-EGFP fusion protein is co-localized with known peroxisomal marker proteins in transfected NIH3T3 cells. In addition, we could demonstrate that the peroxisomal targeting signal NHL is functional and responsible for the correct subcellular localization of the Pxt1-EGFP fusion protein. In male germ cells peroxisomes were reported only in spermatogonia. The Pxt1 gene is so far the first gene coding for a putative peroxisomal protein which is expressed in later steps of spermatogenesis, namely in pachytene spermatocytes.
authors.affiliation: Institute of Human Genetics, University of Göttingen, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Grzmil
authors.forename: C
authors.initials: C
authors.lastname: Burfeind
authors.forename: T
authors.initials: T
authors.lastname: Preuss
authors.forename: C
authors.initials: C
authors.lastname: Dixkens
authors.forename: S
authors.initials: S
authors.lastname: Wolf
authors.forename: W
authors.initials: W
authors.lastname: Engel
authors.forename: P
authors.initials: P
authors.lastname: Burfeind
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
chemical_list: D011506:Proteins; C525857:Pxt1 protein, human; C525856:Pxt1 protein, mouse; D011994:Recombinant Proteins