title: K+-Cl- Cotransporter-3a Up-regulates Na+,K+-ATPase in Lipid Rafts of Gastric Luminal Parietal Cells.
abstract: Gastric parietal cells migrate from the luminal to the basal region of the gland, and they gradually lose acid secretory activity. So far, distribution and function of K+-Cl(-) cotransporters (KCCs) in gastric parietal cells have not been reported. We found that KCC3a but not KCC3b mRNA was highly expressed, and KCC3a protein was predominantly expressed in the basolateral membrane of rat gastric parietal cells located in the luminal region of the glands. KCC3a and the Na+,K+-ATPase alpha1-subunit (alpha1NaK) were coimmunoprecipitated, and both of them were highly localized in a lipid raft fraction. The ouabain-sensitive K+-dependent ATP-hydrolyzing activity (Na+,K+-ATPase activity) was significantly inhibited by a KCC inhibitor (R-(+)-[(2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]acetic acid (DIOA)). The stable exogenous expression of KCC3a in LLC-PK1 cells resulted in association of KCC3a with endogenous alpha1NaK, and it recruited alpha1NaK in lipid rafts, accompanying increases of Na+,K+-ATPase activity and ouabain-sensitive Na+ transport activity that were suppressed by DIOA, whereas the total expression level of alpha1NaK in the cells was not significantly altered. On the other hand, the expression of KCC4 induced no association with alpha1NaK. In conclusion, KCC3a forms a functional complex with alpha1NaK in the basolateral membrane of luminal parietal cells, and it up-regulates alpha1NaK in lipid rafts, whereas KCC3a is absent in basal parietal cells.
authors.affiliation: Department of Pharmaceutical Physiology, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Takuto
authors.initials: T
authors.lastname: Fujii
authors.forename: Yuji
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Itomi
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Fujita
authors.forename: Magotoshi
authors.initials: M
authors.lastname: Morii
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tabuchi
authors.forename: Shinji
authors.initials: S
authors.lastname: Asano
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Tsukada
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takeguchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Sakai
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D005786:Gene Expression Regulation; D006801:Humans; D047468:Immunoprecipitation; D007192:Indenes / Q000494:pharmacology; D021962:Membrane Microdomains / Q000378:metabolism; D010295:Parietal Cells, Gastric / Q000166:cytology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000502:physiology*; D027981:Symporters / Q000502:physiology*; D000096922:K Cl- Cotransporters
chemical_list: D000085:Acetates; D000255:Adenosine Triphosphate; D007192:Indenes; D000254:Sodium-Potassium-Exchanging ATPase; D027981:Symporters; C119650:SLC12A6 protein, human; D000096922:K Cl- Cotransporters; C492281:Slc12a6 protein, rat; C492283:Slc12a7 protein, rat; C095651:((2-n-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy)acetic acid; C499075:Atp1a1 protein, rat