title: Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India.
abstract: BACKGROUND
  Co-circulation of multiple dengue virus serotypes has been reported from many parts of the world including India, however concurrent infection with more than one serotype of dengue viruses in the same individual is rarely documented. An outbreak of dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) occurred in and around Delhi in 2006. This is the first report from India with high percentage of concurrent infections with different dengue virus serotypes circulating during one outbreak.
  
  
  RESULTS
  Acute phase sera from patients were tested for the presence of dengue virus RNA by RT-PCR assay. Of the 69 samples tested for dengue virus RNA, 48 (69.5%) were found to be positive. All the four dengue virus serotypes were found to be co-circulating in this outbreak with DENV-3 being the predominant serotype. In addition in 9 of 48 (19%) dengue virus positive samples, concurrent infection with more than one dengue virus serotype were identified.
  
  
  CONCLUSIONS
  This is the first report in which concurrent infections with different dengue virus serotypes is being reported during an outbreak from India. Delhi is now truly hyperendemic for dengue.
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India. preetibhj@gmail.com
authors.forename: Preeti
authors.initials: P
authors.lastname: Bharaj
authors.forename: Harendra S
authors.initials: HS
authors.lastname: Chahar
authors.forename: Anubhav
authors.initials: A
authors.lastname: Pandey
authors.forename: Kavita
authors.initials: K
authors.lastname: Diddi
authors.forename: Lalit
authors.initials: L
authors.lastname: Dar
authors.forename: Randeep
authors.initials: R
authors.lastname: Guleria
authors.forename: Sushil K
authors.initials: SK
authors.lastname: Kabra
authors.forename: Shobha
authors.initials: S
authors.lastname: Broor
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012703:Serotyping; D019595:Severe Dengue / Q000453:epidemiology* / Q000821:virology
chemical_list: D012367:RNA, Viral