Investigation of antimicrobial model of Hemsleya pengxianensis W.J. Chang and its main active component by metabolomics technique.
OBJECTIVE
H.pengxianensi is a native plant in China. Dihydrocucurbitacin F-25-O-acetate is one of the major chemical components in H.pengxianensis. In this investigation, we attempted to quantify the content of dihydrocucurbitacin F-25-O-acetate in H.pengxianensis and find the possible antibacterial mode of dihydrocucurbitacin F-25-O-acetate and H.pengxianensis.


METHODS
First, the content of dihydrocucurbitacin F-25-O-acetate was quantified by HPLC. Then, metabolic profiles of Staphylococcus aureus treated by dihydrocucurbitacin F-25-O-acetate, extract of H.pengxianensis and nine antibacterial substances with the known modes of action were acquired by HPLC/DAD/ESI-MS. Principal component analysis was carried out upon all metabolic profiles to classify those drugs according to their mechanisms.


CONCLUSIONS
The HPLC result show the content of dihydrocucurbitacin F-25-O-acetate in H.pengxianensis (5.3+/-0.4 mg/g). From the result obtained by principal component analysis, dihydrocucurbitacin F-25-O-acetate was found to be the component playing the main antimicrobial roles on Staphylococcus aureus in H.pengxianensis and the possible antibacterial mode of dihydrocucurbitacin F-25-O-acetate and H.pengxianensis may be to inhibit cell wall synthesis.
Biao-Yi
Zhi
Z
Research Center of Modernization of Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Yu
Yan
Y
Zeng-Liang
Yi
Y
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019662:Cucurbitaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism; D014315:Triterpenes / Q000032:analysis* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D014315:Triterpenes; C098578:dihydrocucurbitacin F