Active immunization against macrophage migration inhibitory factor using a novel DNA vaccine prevents ovariectomy-induced bone loss in mice.
Previous studies have demonstrated that mice deficient in the macrophage migration inhibitory factor (MIF) gene are protected from ovariectomy (OVX)-induced bone loss. We developed a novel MIF-deoxyribonucleic acid (DNA) vaccine by introducing oligonucleotides encoding a helper T epitope into the cDNA sequence of murine MIF. Mice given the MIF-DNA vaccine produced high titers of autoantibody against MIF, and were protected from OVX-induced bone loss. Our results further support the hypothesis that MIF is involved in the pathomechanism of OVX-induced bone loss, and also show that active immunization against MIF using a DNA vaccine may be useful for the prophylactic treatment of postmenopausal osteoporosis.
Onodera
Shin
S
Department of Sports Medicine and Joint Reconstruction Surgery, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan. onodera@med.hokudai.ac.jp
Oshima
Shigeki
S
Nishihira
Jun
J
Yasuda
Kazunori
K
Tohyama
Harukazu
H
Irie
Kazuharu
K
Koyama
Yoshikazu
Y
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D018076:DNA, Complementary; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D005260:Female; D007273:Injections, Intramuscular; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010024:Osteoporosis / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D010052:Ovariectomy / Q000009:adverse effects; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics
D001323:Autoantibodies; D018076:DNA, Complementary; D018984:Epitopes, T-Lymphocyte; D008263:Macrophage Migration-Inhibitory Factors; D019444:Vaccines, DNA