Epigallocatechin-3-gallate induces apoptosis and cell cycle arrest in HTLV-1-positive and -negative leukemia cells.
The objective of this study is to evaluate the efficacy of epigallocatechin gallate against ATL cells. The anti-proliferative and pro-apoptotic effects of EGCG were evaluated in HTLV-1-positive and -negative cells. EGCG exhibited a marked decrease in proliferation of ATL cells at 96 h of treatment. The results indicated that TGF-alpha was down-regulated whereas levels of TGF-beta2 increased. Cell cycle distribution analysis revealed an increase in cells in the pre-G(1) phase which was confirmed by ELISA. The results on proteins showed an up-regulation of p53, Bax and p21 protein levels while the levels of Bcl-2alpha were down-regulated.
Harakeh
S
S
Biology Department, American University of Beirut, Beirut, 11-0236, Lebanon. sharakeh@gmail.com
Abu-El-Ardat
K
K
Diab-Assaf
M
M
Niedzwiecki
A
A
El-Sabban
M
M
Rath
M
M
D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D012333:RNA, Messenger / Q000032:analysis; D015291:Transforming Growth Factors / Q000235:genetics
D012333:RNA, Messenger; D015291:Transforming Growth Factors; D002392:Catechin; C045651:epigallocatechin gallate