title: Changes of mitochondrial pathway in hypoxia/reoxygenation induced cardiomyocytes apoptosis.
abstract: The role of mitochondrial apoptotic pathway in cardiomyocytes subjected to hypoxia/reoxygenation(H/R) was studied. Cultured cardiomyocytes from neonatal Sprague-Dawley rats were exposed to hyoxia/reoxygenation, the apoptotic cardiomyocytes were stained with Annexin-V-FITC, Hoechst 33342 and TUNEL assay. Mitochondrial transmembrane potential of cardiomyocytes was assessed by JC-1 under fluorescence microscope, the expressions of bcl-2, bax, cytochrome c, apoptosis-induced factor (AIF), and caspase-3 were tested by western-blot. Our data showed apoptosis of cardiomyocytes was significantly increased during H/R, accompanied by translocation of bax to mitochondria, release of cytochrome c and AIF to cytosol. The results indicate that the mitochondrial-mediated apoptotic pathway is initiated as a result of H/R.
authors.affiliation: Heart Center, Second Affiliated Hospital, College of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China.
authors.forename: Aina
authors.initials: A
authors.lastname: He
authors.forename: Jian-an
authors.initials: JA
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Gui
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.forename: Tielong
authors.initials: T
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D015687:Cell Hypoxia; D002478:Cells, Cultured; D053078:Membrane Potential, Mitochondrial; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000166:cytology*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010100:Oxygen