title: Identification of a novel Rb-regulated gene associated with the cell cycle.
abstract: The retinoblastoma (Rb) gene is one of the most important genes in cell cycle regulation and tumorigenesis. Homozygosity for a germ-line Rb mutation results in embryonic lethality and evokes developmental defects associated with inappropriate S-phase entry and high levels of apoptosis. Although Rb has been extensively studied, more target genes need to be identified and characterized to unravel the precise mechanism of Rb function. In order to identify Rb-regulated genes, we analyzed the gene expression profile of Rb-deficient mouse embryo fibroblasts (MEFs), and identified an unknown gene, RbEST47, that is transcriptionally upregulated in Rb-deficient MEFs. This gene is conserved from fruitfly to human. It is expressed in brain, lung, kidney, and testis, and is located on mouse chromosome 2. This region is syntenic to human chromosome 9q34.3, which frequently exhibits loss of heterozygosity in neoplastic diseases. RbEST47 was considerably down-regulated in immortalized cells, and showed cell cycle-dependent expression, suggesting important roles in S and/or G2.
authors.affiliation: Department of Biochemistry and Yonsei Laboratory Animal Research Center, Yonsei University, Seoul 120-749, Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Sung
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Han-Woong
authors.initials: HW
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000378:metabolism; D020224:Expressed Sequence Tags; D005347:Fibroblasts / Q000166:cytology; D016161:Genes, Retinoblastoma / Q000235:genetics*; D018816:Genes, cdc; D006801:Humans; D051379:Mice; D016415:Sequence Alignment; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; C527471:RbEST47 protein, mouse