title: The presence of blaIMP genes on plasmids DNA isolated from multidrug-resistant Pseudomonas aeruginosa strains at University Hospital in Bialystok (Poland)--first report.
abstract: Resistance to carbapenems is emerging, and it is a great problem to therapeutics. Seven multidrug-resistant (MDR) of Pseudomonas aeruginosa strains were isolated from urine and bronchial specimens. All isolates showed resistance to imipenem and meropenem (MIC; > or =16 mg/L). The resistance to carbapenems in two of seven strains was associated with the production of a metallo-beta-lactamases. Plasmids DNA probes were used to investigate the presence of genes coding for IMP-type enzymes. PCR experiments revealed that bla(IMP) genes were present in two isolates of Pseudomonas aeruginosa (MIC >32 microg/mL for both carbapenems).
authors.affiliation: Department of Microbiological Diagnostics, Medical University of Bialystok, Poland. sachpt@amb.edu.pl
authors.forename: Paweł
authors.initials: P
authors.lastname: Sacha
authors.forename: Marcin
authors.initials: M
authors.lastname: Zórawski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Hauschild
authors.forename: Piotr
authors.initials: P
authors.lastname: Wieczorek
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Jaworowska
authors.forename: Piotr
authors.initials: P
authors.lastname: Jakoniuk
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Tryniszewska
mesh_terms: D015780:Carbapenems / Q000494:pharmacology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D006785:Hospitals, University; D006801:Humans; D010957:Plasmids / Q000302:isolation & purification*; D011044:Poland; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*
chemical_list: D015780:Carbapenems; D004269:DNA, Bacterial