title: Label-free protein recognition two-dimensional array using nanomechanical sensors.
abstract: We demonstrate two-dimensional multiplexed real-time, label-free antibody-antigen binding assays by optically detecting nanoscale motions of two-dimensional arrays of microcantilever beams. Prostate specific antigen (PSA) was assayed using antibodies covalently bound to one surface of the cantilevers by two different surface chemistries, while the nonreaction surfaces were passivated by poly(ethylene glycol)-silane. PSA as low as 1 ng/mL was detected while 2 mg/microl of bovine serum albumin induced only negligible deflection on the cantilevers.
authors.affiliation: Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.forename: Jeanne C
authors.initials: JC
authors.lastname: Stachowiak
authors.forename: Henry
authors.initials: H
authors.lastname: Lin
authors.forename: Ram
authors.initials: R
authors.lastname: Datar
authors.forename: Richard
authors.initials: R
authors.lastname: Cote
authors.forename: Arun
authors.initials: A
authors.lastname: Majumdar
mesh_terms: D000936:Antigen-Antibody Complex / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D019563:Mechanics; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics / Q000295:instrumentation*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D000936:Antigen-Antibody Complex; D011506:Proteins