Lack of association between the G-2548A polymorphism of the leptin gene and psoriasis in a Turkish population.
BACKGROUND
Psoriasis is a multifactorial disease in which genetic and inflammatory factors play important roles. Leptin is classified as a cytokine and plays an important role in the regulation of the T-helper response. A common polymorphism in the promoter of the human leptin gene (G-2548A) may have a role in the pathogenesis of psoriasis.


OBJECTIVE
To investigate the association between psoriasis and leptin gene polymorphism (G-2548A).


METHODS
The study involved 109 patients with psoriasis and 125 healthy controls. Analyses of G-2548A polymorphism of the leptin gene were made by the polymerase chain reaction-restriction fragment length polymorphism technique. The genotypes (GG, GA, and AA of leptin gene G-2548A) and alleles (G and A) were scored and the frequencies were estimated. The frequencies of alleles and genotypes in patients and controls were compared. The relationship between leptin gene polymorphism and the clinical features of the patients was analyzed.


RESULTS
Both genotype [odds ratio (OR), 0.921; 95% confidence interval (CI), 0.501-1.694; P = 0.792] and allele (OR, 0.864; 95% CI, 0.600-1.242; P = 0.429) frequencies were not significantly different between patient and control groups. In addition, there was no significant association between genotype and allele frequencies and the clinical characteristics of psoriasis.


CONCLUSIONS
In this case-control study, no evidence of association between the G-2548A variant of the leptin gene and psoriasis was found.
Kara
Nurten
N
Department of Medical Biology and Genetics, Ondokuz Mayis University, Samsun, Turkey. nurtenk@omu.edu.tr
Aydin
Fatma
F
Senturk
Nilgün
N
Gunes
Sezgin
S
Canturk
M Tayyar
MT
Bagci
Hasan
H
Bek
Yüksel
Y
Turanli
Ahmet Yasar
AY
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D020738:Leptin / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011565:Psoriasis / Q000235:genetics*; D014421:Turkey
D020738:Leptin