title: URB is abundantly expressed in adipose tissue and dysregulated in obesity.
abstract: Dysregulated production of adipocytokines in obesity is involved in the development of metabolic syndrome. URB/DRO1 contains N-terminal signal sequence and is thought to play a role in apoptosis of tumor cells. In the present study, we investigated the expression pattern of URB mRNA in adipose tissue and secretion from cultured adipocytes. In human and mouse, URB mRNA was predominantly expressed in adipose tissue and was downregulated in obese mouse models, such as ob/ob, KKAy, and diet-induced obese mice. In 3T3L1 adipocytes, insulin, TNF-alpha, H(2)O(2) and hypoxia decreased URB mRNA level. This regulation was similar to that for adiponectin and opposite to MCP-1. URB protein was secreted in media of URB cDNA-stably transfected cells and endogenous URB was detected in media of cultured human adipocytes. In conclusion, the expression pattern of URB suggests its role in obesity and the results suggest that URB is secreted, at least in part, from adipocytes.
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamadaoka, Suita 565-0871, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Okada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nishizawa
authors.forename: Akifumi
authors.initials: A
authors.lastname: Kurata
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tamba
authors.forename: Mina
authors.initials: M
authors.lastname: Sonoda
authors.forename: Atsutaka
authors.initials: A
authors.lastname: Yasui
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kuroda
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hibuse
authors.forename: Norikazu
authors.initials: N
authors.lastname: Maeda
authors.forename: Shinji
authors.initials: S
authors.lastname: Kihara
authors.forename: Tohru
authors.initials: T
authors.lastname: Hadama
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tobita
authors.forename: Suguru
authors.initials: S
authors.lastname: Akamatsu
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Maeda
authors.forename: Iichiro
authors.initials: I
authors.lastname: Shimomura
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
mesh_terms: D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism*; D009928:Organ Specificity; D013045:Species Specificity; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins
chemical_list: C492631:CCDC80 protein, human; C448780:Ccdc80 protein, mouse; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D025521:Tumor Suppressor Proteins