[Polymorphism of five short tandem repeat systems in Chinese Han population in Chengdu].
OBJECTIVE
To obtain population genetic data of loci D11S4951, D11S4957, GATA193H05, D2S2951, and D6S2421 in Han population in Chengdu area and to validate the value of their forensic application.


METHODS
Blood samples were collected in EDTA tubes from unrelated individuals. DNAs were extracted with Chelex-100 and were analyzed by PCR and horizontal PAGE followed by silver staining.


RESULTS
Alleles 7, 10, 8, 6 and 8 were found in 5 STR loci, respectively. No deviations from Hardy-Weinberg balance were observed. The heterozygosities observed were 0.743, 0.772, 0.833, 0.650 and 0.800, respectively. The chances of exclusion were 0.497, 0.549, 0.662, 0.356 and 0.599, and the discrimination powers were 0.863, 0.912, 0.947, 0.829 and 0.931.


CONCLUSIONS
All of the five loci studied may be useful markers for individual identification and paternity testing.
Li
Ying
Y
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. habaer_cn@sina.com
Luo
Hai-bo
HB
Song
Yan-hua
YH
Chen
Xiao
X
Long
Bin
B
Chen
Guo-di
GD
D000483:Alleles; D044466:Asian People / Q000235:genetics; D002681:China / Q000208:ethnology; D004591:Electrophoresis, Polyacrylamide Gel; D005554:Forensic Medicine; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011902:Rape; D020080:Tandem Repeat Sequences / Q000235:genetics*
D005819:Genetic Markers