SarA of Staphylococcus aureus binds to the sarA promoter to regulate gene expression.
The 375-bp sarA open reading frame is driven by three promoters, P1, P3, and P2. Using gel shift and DNase I footprinting assays, we found that SarA binds to two 26-bp sequences and one 31-bp sequence within the P1 and P3 promoters, respectively. Together with the results of transcription analyses, our data indicate that SarA binds to its own promoter to down-regulate sarA expression.
Cheung
Ambrose L
AL
Department of Microbiology and Immunology, Vail 210, Dartmouth Medical School, Hanover, NH 03766, USA. Ambrose.Cheung@Dartmouth.edu
Nishina
Koren
K
Manna
Adhar C
AC
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; C088021:SarA protein, bacterial; D015534:Trans-Activators