[Expression of proliferating cell nuclear antigen in severely damaged small intestinal mucosa due to high-dose 5-FU exposure].
OBJECTIVE
To detect the expression of proliferating cell nuclear antigen (PCNA) in severely damaged intestinal mucosa due to high-dose 5-FU exposure.


METHODS
Thirty-two adult C57BL/6J mice were subjected to daily intraperitoneal high-dose 5-FU injection at 150 mg/kg for 5 consecutive days, and on days 1, 3, and 5, the mice were sacrificed to obtain the small intestinal tissue for HE straining and immunohistochemistry for detecting PCNA expression. Another 8 mice with intraperitoneal PBS injection served as the control group.


RESULTS
High-dose 5-FU exposure of the mice resulted in severe intestinal mucous damage, with complete destruction of the villi and crypts and significantly increased cells positive for PCNA expression (P<0.01).


CONCLUSIONS
High-dose 5-FU treatment can significantly increase the PCNA index, and the cells expressing PCNA can be closely associated with regeneration of the severely damaged mucosa due to the exposure.
Luo
Yu-qi
YQ
Department of General Surgery, Southern Medical University, Guangzhou 510515, China.
Wu
Cheng-tang
CT
Wen
Ying
Y
Liao
Kang-xiong
KX
Zhang
Jun-hua
JH
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000009:adverse effects*; D005472:Fluorouracil / Q000009:adverse effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*
D000964:Antimetabolites, Antineoplastic; D018809:Proliferating Cell Nuclear Antigen; D005472:Fluorouracil