[The changes of stem cells, immune cells and cytokines in peripheral blood after marrow stem cell mobilization in macaqne].
OBJECTIVE
To explore the changes of white blood cells(WBC), neutrophils, stem cells, immune cells and cytokines after marrow stem cell mobilization by GM-CSF in macaqne.


METHODS
Macaqne were injected with GM-CSF 8 mug/(kg.d) for 5 days. The ratio, number and subpopulation of WBC were observed by Blood Cell Autoanalyzer; the ratios of CD133(+), CD34(+), CD3(+), CD4(+), CD8(+), CD56(+) cells were identified by FCM; and levels of TNF-alpha, IL-1beta, IL-2 were tested by ELISA on 0, 2, 4, 6, 8, 10 day after stem cell mobiliation by GM-CSF.


RESULTS
The ratios and number of CD133(+) cells, CD34(+) cells, WBC and neutrophils were significantly increased (P<0.01). The number of above cells was elevated to 6.4, 9.1, 117 and 163.3 times of normal at 6 day after stem cell were mobilized, but the ratios of CD133(+) cells and CD34(+) cell were decreased to the normal level after stem cell mobilization stopped while that of WBC and neutrocytes kept increasing over 8 d . The ratios of CD3(+), CD4(+), CD8(+), CD16(+) were decreased during 1 to 6 d, but came to the normal level at 8 d and increased at 10 d after stem cell were mobilized and the number of those cells in blood increased to 4.1, 4.0, 2.9 and 4.3 times when compared with the normal level. The concentration of TNF-alpha, IL-1beta, IL-2 in peripheral blood was significantly increased and IL-2 level was increased higher and longer than that of TNF-alpha and IL-1beta.


CONCLUSIONS
CD133(+), CD34(+) cells and WBC, neutrophils in peripheral blood are greatly increased after GM-CSF mobilization, but WBC and neutrophils keep increasing longer than that of CD133(+), CD34(+) cells. The results of the number of CD3(+), CD4(+), CD8(+), CD16(+) cells and the levels of TNF-alpha, IL-1beta, IL-2 in peripheral blood indicate that marrow stem cell mobilization by GM-CSF can also stimulate immune cell proliferation and differentiation while increasing cytokine producing.
Pan
Xing-hua
XH
Department of Clinic Experimental, Kunming General Hospital, Kunming 650032, China. xinghuapan@yahoo.com.cn
Pang
Rong-qing
RQ
He
Zhan-long
ZL
Wang
Hui-xian
HX
Zhang
Bu-zhen
BZ
Zhao
Yi
Y
D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D001853:Bone Marrow / Q000187:drug effects* / Q000502:physiology; D001854:Bone Marrow Cells / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology / Q000502:physiology*; D016207:Cytokines / Q000097:blood*; D042783:Endothelial Cells / Q000502:physiology; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008251:Macaca; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology
D015703:Antigens, CD; D016207:Cytokines