title: Coenzyme Q10 protect against ischemia/reperfusion induced biochemical and functional changes in rabbit urinary bladder.
abstract: OBJECTIVE
  Ischemia, reperfusion, and free radical generation have been recently implicated in the progressive bladder dysfunction. Coenzyme Q10 (CoQ10) is a pro-vitamin like substance that appears to be efficient for treatment of neurodegenerative disorders and ischemic heart disease. Our goal was to investigate the potential protective effect of CoQ10 in a rabbit model of in vivo bilateral ischemia and ischemia/reperfusion (I/R).
  
  
  METHODS
  Six groups of four male New Zealand White rabbits each were treated with CoQ10 (3 mg/kg body weight/day-dissolved in peanut oil) (groups 1-3) or vehicle (peanut oil) (groups 4-6). Groups 1 and 4 (ischemia-alone groups) had clamped bilateral vesical arteries for 2 h; in groups 2 and 5 (I/R groups), bilateral ischemia was similarly induced and the rabbits were allowed to recover for 2 weeks. Groups 3 and 6 were controls (shams) and were exposed to sham surgery. The effects on contractile responses to various stimulations and biochemical studies such as citrate synthase (CS), choline acetyltransferase (ChAT), superoxide dismutase (SOD), and catalase (CAT) were evaluated. The protein peroxidation indicator, carbonyl group, and nitrotyrosine contents were analyzed by Western blotting.
  
  
  RESULTS
  Ischemia resulted in significant reductions in the contractile responses to all forms of stimulation in vehicle-fed rabbits, whereas there were no reductions in CoQ10-treated rabbits. Contractile responses were significantly reduced in vehicle-treated I/R groups, but significantly improved in CoQ10-treated rabbits. Protein carbonylation and nitration increased significantly in ischemia-alone and I/R bladders; CoQ10 treatment significantly attenuated protein carbonylation and nitration. CoQ10 up-regulated SOD and CAT activities in control animals; the few differences in CoQ10-treated animal in SOD and CAT after ischemia and in general increase CAT activities following I/R.
  
  
  CONCLUSIONS
  CoQ10 supplementation provides bladder protection against I/R injury. This protection effect improves mitochondrial function during I/R by repleting mitochondrial CoQ10 stores and potentiating their antioxidant properties.
authors.affiliation: Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan.
authors.forename: Yung-Shun
authors.initials: YS
authors.lastname: Juan
authors.forename: Tasmina
authors.initials: T
authors.lastname: Hydery
authors.forename: Anita
authors.initials: A
authors.lastname: Mannikarottu
authors.forename: Barry
authors.initials: B
authors.lastname: Kogan
authors.forename: Catherine
authors.initials: C
authors.lastname: Schuler
authors.forename: Robert E
authors.initials: RE
authors.lastname: Leggett
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Lin
authors.forename: Chun-Hsiung
authors.initials: CH
authors.lastname: Huang
authors.forename: Robert M
authors.initials: RM
authors.lastname: Levin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D019587:Dietary Supplements; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D011817:Rabbits; D015427:Reperfusion Injury / Q000188:drug therapy / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*
chemical_list: D000975:Antioxidants; D014451:Ubiquinone; D002374:Catalase; D013482:Superoxide Dismutase; D002795:Choline O-Acetyltransferase; D002950:Citrate (si)-Synthase; C024989:coenzyme Q10