Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase.
Interleukin 1 (IL-1) has been reported to stimulate the polyubiquitination and disappearance of IL-1 receptor-associated kinase 1 (IRAK1) within minutes. It has been thought that the polyubiquitin chains attached to IRAK1 are linked via Lys48 of ubiquitin, leading to its destruction by the proteasome and explaining the rapid IL-1-induced disappearance of IRAK1. In this paper, we demonstrate that IL-1 stimulates the formation of K63-pUb-IRAK1 and not K48-pUb-IRAK1 and that the IL-1-induced disappearance of IRAK1 is not blocked by inhibition of the proteasome. We also show that IL-1 triggers the interaction of K63-pUb-IRAK1 with NEMO, a regulatory subunit of the IkappaBalpha kinase (IKK) complex, but not with the NEMO[D311N] mutant that cannot bind K63-pUb chains. Moreover, unlike wild-type NEMO, the NEMO[D311N] mutant was unable to restore IL-1-stimulated NF-kappaB-dependent gene transcription to NEMO-deficient cells. Our data suggest a model in which the recruitment of the NEMO-IKK complex to K63-pUb-IRAK1 and the recruitment of the TAK1 complex to TRAF6 facilitate the TAK1-catalyzed activation of IKK by the TRAF6-IRAK1 complex.
Windheim
Mark
M
MRC Protein Phosphorylation Unit, College of Life Sciences, The Sir James Black Centre, University of Dundee, Dow Street, Dundee DD1 5EH, Scotland, United Kingdom.
Stafford
Margaret
M
Peggie
Mark
M
Cohen
Philip
P
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004789:Enzyme Activation; D005347:Fibroblasts / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology*; D053592:Interleukin-1 Receptor-Associated Kinases / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000166:cytology; D049409:Luciferases, Firefly / Q000032:analysis / Q000378:metabolism; D049410:Luciferases, Renilla / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D009154:Mutation; D011485:Protein Binding; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism; D014162:Transfection; D054875:Ubiquitination
D007375:Interleukin-1; D047908:Intracellular Signaling Peptides and Proteins; C514981:NEMO protein, mouse; D048029:TNF Receptor-Associated Factor 6; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly; D053592:Interleukin-1 Receptor-Associated Kinases; D051550:I-kappa B Kinase; D008239:Lysine