title: Strategy for in situ detection of natural transformation-based horizontal gene transfer events.
abstract: A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.
authors.affiliation: Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy.
authors.forename: Aurora
authors.initials: A
authors.lastname: Rizzi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pontiroli
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Brusetti
authors.forename: Sara
authors.initials: S
authors.lastname: Borin
authors.forename: Claudia
authors.initials: C
authors.lastname: Sorlini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Abruzzese
authors.forename: Gian Attilio
authors.initials: GA
authors.lastname: Sacchi
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Vogel
authors.forename: Pascal
authors.initials: P
authors.lastname: Simonet
authors.forename: Marco
authors.initials: M
authors.lastname: Bazzicalupo
authors.forename: Kaare Magne
authors.initials: KM
authors.lastname: Nielsen
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Monier
authors.forename: Daniele
authors.initials: D
authors.lastname: Daffonchio
mesh_terms: D000150:Acinetobacter / Q000235:genetics*; D022761:Gene Transfer, Horizontal / Q000235:genetics*; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D014169:Transformation, Bacterial / Q000235:genetics*
chemical_list: D049452:Green Fluorescent Proteins