Control of 5-FOA and 5-FU resistance by Saccharomyces cerevisiae YJL055W.
In a URA3/5-FOA-based dosage-suppressor screen, we isolated a plasmid containing the little-characterized ORF YJL055W. Further analysis showed that this gene did not suppress the mutation of interest. Instead, overexpression of Yjl055Wp directly suppressed the non-viability of URA3(+) cells in the presence of 5-FOA. Overexpression of Yjl055Wp also suppressed the lethality induced by 5-FU, but deletion of YJL055W had no detectable effect on resistance to either 5-FOA or 5-FU. Based on these observations and a previous report that a yjl055wDelta mutant has increased sensitivity to purine-analogue mutagens, we suggest that Yjl055Wp may function in one of several pathways for the detoxification of base analogues. However, its precise mechanism of action remains unknown.
Ko
Nolan
N
Department of Biology, University of North Carolina, Chapel Hill, NC, USA.
Nishihama
Ryuichi
R
Pringle
John R
JR
D000935:Antifungal Agents / Q000494:pharmacology; D025141:Drug Resistance, Fungal / Q000502:physiology*; D005472:Fluorouracil / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal; D009963:Orotic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D000935:Antifungal Agents; D029701:Saccharomyces cerevisiae Proteins; C531894:YJL055w protein, S cerevisiae; D009963:Orotic Acid; C001242:5-fluoroorotic acid; D005472:Fluorouracil