title: Applicability of phylogenetic methods for characterizing the public health significance of verocytotoxin-producing Escherichia coli strains.
abstract: Two phylogenetic methods (multilocus sequence typing [MLST] and a multiplex PCR) were investigated to determine whether phylogenetic classification of verocytotoxin-producing Escherichia coli serotypes correlates with their classification into groups (seropathotypes A to E) based on their relative incidence in human disease and on their association with outbreaks and serious complications. MLST was able to separate 96% of seropathotype D and E serotypes from those that cause serious disease (seropathotypes A to C), whereas the multiplex PCR lacked this level of seropathotype discrimination.
authors.affiliation: Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada.
authors.forename: Kim
authors.initials: K
authors.lastname: Ziebell
authors.forename: Paulina
authors.initials: P
authors.lastname: Konczy
authors.forename: Irene
authors.initials: I
authors.lastname: Yong
authors.forename: Shelley
authors.initials: S
authors.lastname: Frost
authors.forename: Mariola
authors.initials: M
authors.lastname: Mascarenhas
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Kropinski
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Whittam
authors.forename: Susan C
authors.initials: SC
authors.lastname: Read
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Karmali
mesh_terms: D001483:Base Sequence; D016000:Cluster Analysis; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D005798:Genes, Bacterial / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011634:Public Health / Q000379:methods; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D022622:Shiga Toxin 1 / Q000378:metabolism*; D013045:Species Specificity; D014774:Virulence
chemical_list: D022622:Shiga Toxin 1