title: Comparison of retropharyngeal lymph node and obex region of the brainstem in detection of chronic wasting disease in white-tailed deer (Odocoileus virginianus).
abstract: Chronic wasting disease (CWD) in Wisconsin was first identified in February 2002. By April 2005, medial retropharyngeal lymph node (RLN) tissues had been examined from over 75,000 white-tailed deer for the presence of CWD by either immunohistochemical (IHC) staining for the prion protein associated with CWD (PrP(res)) or by using enzyme-linked immunosorbent assays with confirmation of positives by IHC staining and had been detected in 469 animals. Obex tissue was also available from 438 of the CWD-positive animals and was CWD positive by IHC staining in 355 (81%). To verify whether false-negative results were possible examining only RLN, both obex and RLN samples were examined for CWD by IHC staining from 4,430 of the white-tailed deer harvested from an area in Wisconsin where the overall deer CWD prevalence was approximately 6.2%. Two hundred and fourteen of the 269 positive deer (79.6%) had deposits of PrP(res) in both obex and lymphoid tissues, 55 (20.4%) had deposits only in lymphoid tissue, and there were no deer that had deposits only in obex.
authors.affiliation: University of Wisconsin, 445 Easterday Lane, Madison, WI 53706, USA. Delwyn.Keane@wvdl.wisc.edu
authors.forename: Delwyn P
authors.initials: DP
authors.lastname: Keane
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Barr
authors.forename: Jason E
authors.initials: JE
authors.lastname: Keller
authors.forename: S Mark
authors.initials: SM
authors.lastname: Hall
authors.forename: Julie A
authors.initials: JA
authors.lastname: Langenberg
authors.forename: Philip N
authors.initials: PN
authors.lastname: Bochsler
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000473:pathology*; D003670:Deer; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D008198:Lymph Nodes / Q000473:pathology*; D034081:Wasting Disease, Chronic / Q000175:diagnosis / Q000473:pathology*