title: Subthalamic stimulation increases striatal tyrosine hydroxylase phosphorylation.
abstract: Subthalamic stimulation enhances striatal tyrosine hydroxylase activity, which is regulated by phosphorylation at different serine residues. Western blotting was performed to investigate phosphorylation at the serine residues 19, 31 and 40 in striatal tissue of rats that had received subthalamic stimulation or sham stimulation for 2 h. In animals that were killed directly after stimulation, the tyrosine hydroxylase protein content was unchanged, whereas phosphorylation at the serine residue 19 was increased and phosphorylation at the serine residues 31 and 40 tended to be higher compared with controls. By contrast, tyrosine hydroxylase protein content and phosphorylation were similar in rats that were killed 24 h after stimulation. Our results suggest that subthalamic stimulation may increase tyrosine hydroxylase activity via increased phosphorylation.
authors.affiliation: CNRS UMR 5227, Bordeaux, France.
authors.forename: Rene
authors.initials: R
authors.lastname: Reese
authors.forename: Christine
authors.initials: C
authors.lastname: Winter
authors.forename: Agnes
authors.initials: A
authors.lastname: Nadjar
authors.forename: Daniel
authors.initials: D
authors.lastname: Harnack
authors.forename: Rudolf
authors.initials: R
authors.lastname: Morgenstern
authors.forename: Andreas
authors.initials: A
authors.lastname: Kupsch
authors.forename: Erwan
authors.initials: E
authors.lastname: Bezard
authors.forename: Wassilios
authors.initials: W
authors.lastname: Meissner
mesh_terms: D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D001665:Binding Sites; D003342:Corpus Striatum / Q000033:anatomy & histology / Q000201:enzymology*; D004298:Dopamine / Q000096:biosynthesis*; D004558:Electric Stimulation; D004599:Electric Stimulation Therapy; D008297:Male; D009434:Neural Pathways / Q000033:anatomy & histology / Q000201:enzymology; D010300:Parkinson Disease / Q000201:enzymology / Q000503:physiopathology / Q000628:therapy; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D012694:Serine / Q000378:metabolism; D020531:Subthalamic Nucleus / Q000033:anatomy & histology / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology
chemical_list: D012694:Serine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine