Expression and characterization of two STAT isoforms from Sf9 cells.
In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
Yeh
Maw-Sheng
MS
Department of Food and Nutrition, Hung-Kuang University, Taichung, Taiwan.
Cheng
Chia-Hsiung
CH
Chou
Chih-Ming
CM
Hsu
Ya-Li
YL
Chu
Cheng-Ying
CY
Chen
Gen-Der
GD
Chen
Shui-Tsung
ST
Chen
Guang-Chao
GC
Huang
Chang-Jen
CJ
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D050791:STAT Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera; D015533:Transcriptional Activation / Q000235:genetics; D048490:raf Kinases / Q000235:genetics / Q000378:metabolism
D020033:Protein Isoforms; D050791:STAT Transcription Factors; D004247:DNA; D048490:raf Kinases