LC MALDI-TOF MS/MS and LC ESI FTMS analyses of HLA-B27 associated peptides isolated from peripheral blood cells.
Peptides eluted from peripheral blood cells of HLA-B*2705 healthy donor were analyzed by LC MALDI MS/MS and LC ESI FTMS techniques. The sequences of 92 peptide ligands identified from one healthy blood donor by LC MALDI-TOF MS/MS were compared with those previously published from in vitro long-term cell cultures available in SYFPEITHI database and splenocytes. It was found that 18 sequences confirmed within 1ppm mass error by LC ESI FTMS were already described and 3 of them matched with those previously reported from HLA-B*2705 splenocytes. Another 38 sequences validated within the same mass error were not found in SYFPEITHI database and are identified here for the first time. Finally, 36 sequences (5 sequences already published in SYFPEITHI database) were evaluated by LC MALDI-TOF MS/MS but no matches in the list of monoisotopic masses obtained from LC ESI FTMS were found.
Stodůlková
Eva
E
Department of Biogenesis and Biotechnology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 14220 Prague 4, Czech Republic.
Novák
Petr
P
Deininger
Sören-Oliver
SO
Man
Petr
P
Capková
Jana
J
Kavan
Daniel
D
Ivasková
Eva
E
Flieger
Miroslav
M
D000328:Adult; D000429:Alcohol Oxidoreductases; D015551:Autoimmunity / Q000235:genetics; D001773:Blood Cells / Q000276:immunology / Q000378:metabolism; D030562:Databases, Protein; D010450:Endopeptidases / Q000378:metabolism; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000097:blood / Q000235:genetics / Q000276:immunology; D020022:Genetic Predisposition to Disease; D015796:HLA-B27 Antigen / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D050883:HSC70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008297:Male; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D054730:Protein Interaction Domains and Motifs; D016415:Sequence Alignment; D012984:Software; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013167:Spondylitis, Ankylosing / Q000235:genetics / Q000378:metabolism
D015796:HLA-B27 Antigen; D050883:HSC70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D006657:Histones; D010455:Peptides; D000429:Alcohol Oxidoreductases; C021379:D-2-hydroxyacid dehydrogenase; D010450:Endopeptidases; D019205:GTP-Binding Protein alpha Subunits, Gs