[mRNA and protein expression of haptoglobin in lesions of condyloma acuminatum].
OBJECTIVE
To investigate the expression of haptoglobin in the lesions of condyloma acuminatum (CA) at the mRNA and protein level, and to explore its role in the pathogenesis of CA.


METHODS
The expressions of haptoglobin protein and mRNA in the skin tissues of 30 patients with CA and 20 normal controls were detected by immunohistochemistry(IHC), Western blot, and hybridization in situ.


RESULTS
The in situ hybridization study showed that haptoglobin mRNA was expressed in the epidermal cells in the lesions of CA. The distribution of haptoglobin mRNA expression in the lesions of CA was similar to that of the normal controls, and the expression of haptoglobin mRNA in CA was higher than that of the normal controls. There was a significant difference in the positive expression of haptoglobin mRNA between the CA group and the control group (P<0.05). The immunohistochemical study showed that haptoglobin protein was expressed in the whole layers of epidermal keratinocytes in the lesions of CA at a high level and stronger staining was seen in the stratum basale and stratum spinosum. Haptoglobin protein was expressed predominantly in the stratum basale in normal skin tissues, while weak staining was seen below the stratum spinosum.There was a significant difference in the mean gray value between the CA group and control group (P<0.05). Western blot showed that the haptoglobin expression in CA lesions significantly increased compared with the normal skins (P<0.05).


CONCLUSIONS
The expression of haptoglobin mRNA in the CA lesions obviously increases and the epidermal cells in the CA lesions are able to synthesize haptoglobin protein. Haptoglobin in the CA lesions may involve in the local immunity escape by preventing Langerhans cell functional maturation and inhibiting the immunocompetence of keratinocyte.
Lu
Jian-yun
JY
Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, China. kateljy@yahoo.com.cn
Wu
Zhi-qiang
ZQ
Tan
Li-na
LN
Chen
Jing
J
Xiang
Ya-ping
YP
Zuo
Chen-xin
CX
Huang
Jin-hua
JH
Jiang
Xian-zhen
XZ
D000293:Adolescent; D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D003218:Condylomata Acuminata / Q000235:genetics / Q000378:metabolism*; D000078404:Epidermal Cells; D006242:Haptoglobins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
D006242:Haptoglobins; D012333:RNA, Messenger