title: Electrochemical detection of kinase-catalyzed phosphorylation using ferrocene-conjugated ATP.
abstract: Adenosine-5'-[gamma-ferrocene] triphosphate is exploited as a co-substrate for the phosphorylation of the surface-immobilized peptide C-SIYRRGSRRWRKL by protein kinase C, in which the gamma-ferrocene phosphate is transferred to the peptide and then detected by cyclic voltammetry.
authors.affiliation: Department of Chemistry, University of Western Ontario, London, Ontario, Canada.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Song
authors.forename: Kagan
authors.initials: K
authors.lastname: Kerman
authors.forename: Heinz-Bernhard
authors.initials: HB
authors.lastname: Kraatz
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D013058:Mass Spectrometry; D000075163:Metallocenes; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; C004998:ferrocene