Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery.
Cationic polyorganophosphazene has shown the ability to deliver gene. To obtain more efficient transfection, His(Boc)-OMe bearing histidine moiety was introduced to synthesize a new derivative of cationic polyphosphazenes with another side group of 2-dimethylaminoethylamine (DMAEA). The poly(DMAEA/His(Boc)-OMe)phosphazene (PDHP) and DNA could self-assemble into nanoparticles with a size around 110 nm and zeta potential of +15 mV at the PDHP/DNA ratio of 10:1 (w/w). The maximum transfection efficiency of PDHP/DNA self-assembled nanoparticles (PHSNs) against 293 T cells was much higher than that of poly(di-2-dimethylaminoethylamine) phosphazenes (PDAP)/DNA self-assembled nanoparticles (PASNs) and PEI 25/DNA self-assembled nanoparticles (PESNs) at the polymer/DNA ratio of 10:1, but the cytotoxicity of PDHP assayed by MTT was much lower than that of PDAP and PEI 25. These results suggested that PDHP could be a good candidate with high transfection efficiency and low cytotoxicity for gene delivery.
Yang
Yongxin
Y
Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, China.
Xu
Zhenghong
Z
Chen
Shangwei
S
Gao
Yu
Y
Gu
Wangwen
W
Chen
Lingli
L
Pei
Yuanying
Y
Li
Yaping
Y
D045744:Cell Line, Tumor; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage*; D018014:Gene Transfer Techniques; D006801:Humans; D053758:Nanoparticles; D009943:Organophosphorus Compounds / Q000008:administration & dosage*; D010316:Particle Size; D011108:Polymers / Q000008:administration & dosage*; D014162:Transfection
D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene); D004247:DNA