MRI detection of thrombin with aptamer functionalized superparamagnetic iron oxide nanoparticles.
Design of smart MRI contrast agent based on superparamagnetic iron oxide nanoparticles and aptamers has been described for the detection of human alpha-thrombin protein. The contrast agent is based on the assembly of the aptamer functionalized nanoparticles in the presence of thrombin. A detectable change in MRI signal is observed with 25 nM thrombin in human serum. Changes were neither observed with control analytes, streptavidin, or bovine serum albumin, nor with inactive aptamer functionalized nanoparticles.
Yigit
Mehmet Veysel
MV
Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Mazumdar
Debapriya
D
Lu
Yi
Y
D001483:Base Sequence; D017931:DNA Primers; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D053768:Metal Nanoparticles; D013917:Thrombin / Q000032:analysis*
D017931:DNA Primers; D005290:Ferric Compounds; C000499:ferric oxide; D013917:Thrombin