Effective gene transfer to melanoma cells using bacterial ghosts.
Bacterial ghosts (BG) are cell envelopes preparations of Gram-negative bacteria devoid of cytoplasmic content produced by controlled expression of PhiX174 plasmid-encoded lysis gene E. Eight melanoma cell lines were investigated for their capacity to bind and phagocyte BG derived from Escherichia coli NM522 and Mannheimia haemolytica A23. High capability to bind BG was observed in almost all of the analyzed cell lines, furthermore cells were able to take up BG independently of the used bacterial species. Further, transfection efficiency of BG loaded with DNA in vitro was measured. The Bowes cells exhibited a high expression level of GFP and the incubation of cells with plasmid loaded BG led up to 82% transfection efficiency.
Kudela
Pavol
P
Cancer Research Institute, Slovak Academy of Sciences, Vlarska, Bratislava, Slovakia. pavol.kudela@savba.sk
Paukner
Susanne
S
Mayr
Ulrike Beate
UB
Cholujova
Dana
D
Kohl
Gudrun
G
Schwarczova
Zuzana
Z
Bizik
Jozef
J
Sedlak
Jan
J
Lubitz
Werner
W
D045744:Cell Line, Tumor; D002462:Cell Membrane; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D016978:Mannheimia haemolytica / Q000235:genetics*; D008545:Melanoma / Q000235:genetics*; D010587:Phagocytosis; D010957:Plasmids; D012878:Skin Neoplasms / Q000235:genetics*; D014162:Transfection
D049452:Green Fluorescent Proteins