Bioimaging of dexamethasone and TGF beta-1 and its biological activities of chondrogenic differentiation in hydrogel constructs.
This study examined the efficacy of poly(NiPAAm-co-AAc) as an injectable drug delivery vehicle and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. The hydrogel constructs, which consisted of embedded cells co-encapsulating dexamethasone (Dex) and TGF beta-1 or unloaded Dex, were used as controls to determine the effects of Dex and TGF beta-1 on chondrogenic differentiation. The level of Dex and TGF beta-1 released was monitored using a bioimaging method. The amount of Dex released from hydrogel was faster than that of TGF beta-1. TGF beta-1 was present in hydrogel for more than 4 weeks after the injection. The level of the cartilage associated ECM proteins was examined by immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. These results highlight the potential of a thermo-reversible hydrogel mixed with the chondrocytes and differentiation delivery material for applications in neocartilage formation.
Na
Kun
K
Division of Biotechnology, The Catholic University of Korea, Wonmi-gu, Bucheon 420-743, Korea.
Kim
Sungwon
S
Sun
Bo Kyung
BK
Woo
Dae Gyun
DG
Yang
Han Na
HN
Chung
Hyung-Min
HM
Park
Keun-Hong
KH
D000180:Acrylic Resins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D020219:Chondrogenesis / Q000187:drug effects*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D003907:Dexamethasone / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005938:Glucocorticoids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D000180:Acrylic Resins; D001672:Biocompatible Materials; C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D024043:Collagen Type II; D004337:Drug Carriers; D005456:Fluorescent Dyes; D005938:Glucocorticoids; D020100:Hydrogels; D053773:Transforming Growth Factor beta1; C469412:poly(N-isopropylacrylamide-co-acrylamide); D003907:Dexamethasone; D016650:Fluorescein-5-isothiocyanate