title: A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.
abstract: An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels directly from cell culture media in contrast to previous reports that required extensive purification and proteolytic digestion. The sulfation site was not previously described nor predicted by informatic analysis of the peptide's precursor sequence.
authors.affiliation: Amylin Pharmaceuticals, Inc., 9360 Towne Centre Drive, San Diego, California 92121, USA. staylor@amylin.com
authors.forename: Steven W
authors.initials: SW
authors.lastname: Taylor
authors.forename: Chengzao
authors.initials: C
authors.lastname: Sun
authors.forename: Amy
authors.initials: A
authors.lastname: Hsieh
authors.forename: Nancy L
authors.initials: NL
authors.lastname: Andon
authors.forename: Soumitra S
authors.initials: SS
authors.lastname: Ghosh
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D053378:Chromogranin B / Q000737:chemistry* / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D004285:Dogs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000378:metabolism; D010748:Phosphopeptides / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000737:chemistry; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013463:Sulfuric Acid Esters / Q000737:chemistry* / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000235:genetics
chemical_list: D053378:Chromogranin B; D017077:Culture Media, Conditioned; D009479:Neuropeptides; D010748:Phosphopeptides; D013463:Sulfuric Acid Esters; C529325:chrombacin h peptide, rat; D010768:Phosphoserine; C005662:tyrosine O-sulfate; D014443:Tyrosine