title: Toxoplasma gondii and Cryptosporidium parvum lack detectable DNA cytosine methylation.
abstract: Epigenetic factors play a role in the expression of virulence traits in Apicomplexa. Apicomplexan genomes encode putative DNA cytosine methylation enzymes. To assess the presence of cytosine methylation of Toxoplasma gondii and Cryptosporidium parvum DNA, we used mass spectrometry analysis and confirmed that these organisms lack detectable methylcytosine in their DNA.
authors.affiliation: Albert Einstein College of Medicine, Ullmann 1225, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gissot
authors.forename: Sang-Woon
authors.initials: SW
authors.lastname: Choi
authors.forename: Reid F
authors.initials: RF
authors.lastname: Thompson
authors.forename: John M
authors.initials: JM
authors.lastname: Greally
authors.forename: Kami
authors.initials: K
authors.lastname: Kim
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D000818:Animals; D016785:Cryptosporidium parvum / Q000235:genetics*; D019175:DNA Methylation; D016054:DNA, Protozoan / Q000378:metabolism; D013058:Mass Spectrometry; D014122:Toxoplasma / Q000235:genetics*
chemical_list: D016054:DNA, Protozoan; D044503:5-Methylcytosine