title: Bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine.
abstract: Adjuvants play an important role in the formulation of effective and appropriate vaccines. A series of synthetic bursin and bursin-like peptides was heterologously expressed in Escherichia coli. The use of bursin as an adjuvant enhanced the specific immune responses of mice immunized with a recombinant Japanese encephalitis virus E-binding domain (JEV E-BD) fusion protein. The effect was determined in the form of protective anti-JEV E titers, antibodies (IgG1 and IgG2a), spleen cell lymphocyte proliferation, the levels of interferon-gamma and interleukin-4 cytokines, and the T-lymphocyte sub-type composition. The IgG2a titer and interferon-gamma level suggested that the E-BD protein potentiates the Th1 immune response. These responses were changed when the immunogen was combined with one of the synthetic peptides as adjuvant. JEV-neutralization assay results show that the presence of bursin significantly enhance the JEV-neutralizing titer. We conclude that bursin as an adjuvant is a potent enhancer of immune response in mice immunized with the JEV subunit vaccine, and represents a promising adjuvant for vaccination.
authors.affiliation: Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Wen
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Su
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Ge
authors.forename: Zhan-Guo
authors.initials: ZG
authors.lastname: Dang
authors.forename: Xiang-Guo
authors.initials: XG
authors.lastname: Duan
authors.forename: Rui-Bing
authors.initials: RB
authors.lastname: Cao
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.forename: Pu-Yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D022321:Japanese Encephalitis Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D009500:Neutralization Tests; D009842:Oligopeptides / Q000276:immunology*; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000166:cytology
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D022321:Japanese Encephalitis Vaccines; D009842:Oligopeptides; D021122:Protein Subunits; D011994:Recombinant Proteins; C016281:bursopoietin