Presence of quorum-sensing systems associated with multidrug resistance and biofilm formation in Bacteroides fragilis.
Bacteroides fragilis constitutes 1-2% of the natural microbiota of the human digestive tract and is the predominant anaerobic opportunistic pathogen in gastrointestinal infections. Most bacteria use quorum sensing (QS) to monitor cell density in relation to other cells and their environment. In Gram-negative bacteria, the LuxRI system is common. The luxR gene encodes a transcriptional activator inducible by type I acyl-homoserine lactone autoinducers (e.g., N-[3-oxohexanoyl] homoserine lactone and hexanoyl homoserine lactone [C6-HSL]). This study investigated the presence of QS system(s) in B. fragilis. The genome of American-type culture collection strain no. ATCC25285 was searched for QS genes. The strain was grown to late exponential phase in the presence or absence of synthetic C6-HSL and C8-HSL or natural homoserine lactones from cell-free supernatants from spent growth cultures of other bacteria. Growth, susceptibility to antimicrobial agents, efflux pump gene (bmeB) expression, and biofilm formation were measured. Nine luxR and no luxI orthologues were found. C6-HSL and supernatants from Yersinia enterocolitica, Vibrio cholerae, and Pseudomonas aeruginosa caused a significant (1) reduction in cellular density and (2) increases in expression of four putative luxR genes, bmeB3, bmeB6, bmeB7, and bmeB10, resistance to various antibiotics, which was reduced by carbonyl cyanide-m-chlorophenyl hydrazone (CCCP, an uncoupler that dissipates the transmembrane proton gradient, which is also the driving force of resistance nodulation division efflux pumps) and (3) increase in biofilm formation. Susceptibility of ATCC25285 to C6-HSL was also reduced by CCCP. These data suggest that (1) B. fragilis contains putative luxR orthologues, which could respond to exogenous homoserine lactones and modulate biofilm formation, bmeB efflux pump expression, and susceptibility to antibiotics, and (2) BmeB efflux pumps could transport homoserine lactones.
Pumbwe
Lilian
L
Greater Los Angeles Veterans Administration Healthcare Systems, Wadsworth Anaerobe Laboratory, Bldg. 304, Room E3-224, 691/151J, Los Angeles, CA 90073, USA. lilskil@hotmail.com
Skilbeck
Christopher A
CA
Wexler
Hannah M
HM
D054742:Acyl-Butyrolactones / Q000378:metabolism; D001441:Bacteroides fragilis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018441:Biofilms / Q000254:growth & development*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D053038:Quorum Sensing / Q000187:drug effects / Q000502:physiology*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology*; D014475:Uncoupling Agents / Q000494:pharmacology
D054742:Acyl-Butyrolactones; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012097:Repressor Proteins; D015534:Trans-Activators; D014475:Uncoupling Agents; C064778:LuxR autoinducer binding proteins; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone