title: Subunit-subunit interactions in the human 26S proteasome.
abstract: Ubiquitin-dependent proteolysis is mediated by the proteasome. To understand the structure and function of the human 26S proteasome, we cloned complete ORFs of 32 human proteasome subunits and conducted a yeast two-hybrid analysis of their interactions with each other. We observed that there are 114 interacting-pairs in the human 26S proteasome. About 10% (11/114) of these interacting-pairs was confirmed by the GST-pull down analysis. Among these observed interacting subunits, 58% (66/114) are novel and the rest 42% (48/114) has been reported previously in human or in other species. We observed new interactions between the 19S regulatory particle and the beta-rings of the 20S catalytic particle and therefore proposed a modified model of the 26S proteasome.
authors.affiliation: Key Laboratory for Cell Biology and Tumor Cell Engineering, the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
authors.forename: Caoxin
authors.initials: C
authors.lastname: Huang
authors.forename: Shouhui
authors.initials: S
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.forename: Wenbo
authors.initials: W
authors.lastname: Lin
authors.forename: Guifen
authors.initials: G
authors.lastname: Ke
authors.forename: Hongxin
authors.initials: H
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Zeguang
authors.initials: Z
authors.lastname: Han
authors.forename: Lixin
authors.initials: L
authors.lastname: Ma
authors.forename: Keke
authors.initials: K
authors.lastname: Huo
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yang
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
mesh_terms: D006801:Humans; D008954:Models, Biological; D016366:Open Reading Frames / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*
chemical_list: D021122:Protein Subunits; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease