[A study on the time-dependent expression of caspase-6 during repair of skin contusion in rats].
OBJECTIVE
To investigate the expression of caspase-6 in rat skin contusion and its surrounding areas during repairment.


METHODS
Immunohistochemical SP method and Western blot technique were used to study the expression and activation of caspase-6 in rat skin contusion and its surrounding areas.


RESULTS
Weak expression of caspase-6 was detected in cytoplasms of polymorphonuchear cells (PMNs) infiltrated in the injured area at 3 hours post-contusion. The ratio of the caspase-6 positive cells was low (25.78 +/- 1.38)%. The expression of caspase-6 was increased prominently (47.70 +/- 5.14)% at 12 hours post-contusion. Almost all of the PMNs, mononuclear cells (MNCs) and fibroblastic cells (FBCs) were caspase-6 positive with both cytoplasm and nucleus staining (54.58 +/- 5.64)% on post-contusion day 3. The expression of caspase-6 decreased gradually thereafter. The expression of the 34-kDa pro-caspase-6 was detected by Western blot in both control and the post-contusion groups with time dependent dynamics.


CONCLUSIONS
These results suggest that caspase-6 may play a major role in trauma-induced inflammatory response. Since caspase-6 shows a timely dependent expression in PMNs, MNCs and FBCs during skin injury repair in rat, it may be used as a marker for the contusion age determination,
Peng
Xue
X
School of Forensic Medicine, China Medical University, Shenyang 110001, China.
Guan
Da-wei
DW
Wang
Qi
Q
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D053178:Caspase 6 / Q000378:metabolism*; D003288:Contusions / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing
C505518:Casp6 protein, rat; D053178:Caspase 6