Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue.
2-Acetyl-6-(dimethylamino)naphthalene-derived two-photon fluorescent Ca2+ probes (ACa1-ACa3) are reported. They can be excited by a 780 nm laser beam, show 23-50-fold enhancement in one- and two-photon excited fluorescence in response to Ca2+, emit fourfold stronger two-photon excited fluorescence than Oregon Green 488 BAPTA-1 upon complexation with Ca2+, and can selectively detect intracellular free Ca2+ ions in live cells and living tissues with minimum interference from other metal ions and membrane-bound probes. Moreover, these probes are capable of monitoring calcium waves at a depth of 120-170 microm in live tissues for 1100-4000 s using two-photon microscopy with no artifacts of photobleaching.
Kim
Hwan Myung
HM
Molecular Opto-Electronics Laboratory, Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea.
Kim
Bo Ra
BR
An
Myoung Jin
MJ
Hong
Jin Hee
JH
Lee
Kyoung J
KJ
Cho
Bong Rae
BR
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000737:chemistry / Q000493:pharmacokinetics* / Q000528:radiation effects; D020013:Calcium Signaling / Q000502:physiology*; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics* / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007031:Hypothalamus / Q000166:cytology / Q000502:physiology; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods; D015394:Molecular Structure; D017785:Photons; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism; D013997:Time Factors; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D002118:Calcium