title: Crotonkinins A and B and related diterpenoids from Croton tonkinensis as anti-inflammatory and antitumor agents.
abstract: Cytotoxicity-guided phytochemical investigation of a methanolic extract of Croton tonkinensis afforded two new kaurane diterpenoids (1, 2) and 10 known ent-kaurane-type diterpenoids (3- 12). The structures of 1 and 2 were based on analysis of spectroscopic and mass spectral data. Compounds 3- 12 were identified by comparison of their spectroscopic and physical data with those reported in the literature. Selected compounds from this plant were examined for cytotoxic and anti-inflammatory activities. Compounds 4 and 9 showed the highest cytotoxic activity against the tested tumor cell lines. Compounds 3, 4, 6, 8, 9, and 11 had IC 50 values less than 5 microM and were more potent than the nonspecific NOS inhibitor L-NAME in inhibiting LPS-induced NO production.
authors.affiliation: Department of Biotechnology, National Formosa University, Yunlin, Taiwan, ROC.
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Kuo
authors.forename: Yuh-Chiang
authors.initials: YC
authors.lastname: Shen
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Yang
authors.forename: Su-Hui
authors.initials: SH
authors.lastname: Wang
authors.forename: Tran Dinh
authors.initials: TD
authors.lastname: Thang
authors.forename: Nguyen Xuan
authors.initials: NX
authors.lastname: Dung
authors.forename: Po-Cheng
authors.initials: PC
authors.lastname: Chiang
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Lee
authors.forename: E-Jian
authors.initials: EJ
authors.lastname: Lee
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029802:Croton / Q000737:chemistry*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000494:pharmacology; D015394:Molecular Structure; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D014744:Vietnam
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D008070:Lipopolysaccharides; C526842:crotonkinin A; C526843:crotonkinin B; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester