In vivo imaging and evaluation of different biomatrices for improvement of stem cell survival.
Therapeutic effects from injection of stem cells are often hampered by acute donor cell death as well as migration away from damaged areas. This is likely due to the fact that injected cells do not have the physical and biochemical cues for ordered engrafment. Here we evaluate 3 common biomatrices (Matrigel, Collagen I, Purmatrix) that has the potential of providing suitable scaffolds needed to enhance stem cell survival. The longitudinal fate of transplanted stem cells was monitored by reporter imaging techniques.
Cao
Feng
F
Department of Radiology and Molecular Imaging Program at Stanford (MIPS), Stanford, CA, USA.
Sadrzadeh Rafie
Amir H
AH
Abilez
Oscar J
OJ
Wang
Haichang
H
Blundo
Jennifer T
JT
Pruitt
Beth
B
Zarins
Christopher
C
Wu
Joseph C
JC
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism* / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D008163:Luminescent Measurements; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D051598:Whole Body Imaging / Q000379:methods*
D001672:Biocompatible Materials