The cytoplasmic domain of chondrolectin interacts with the beta-subunit of Rab geranylgeranyl transferase.
Mouse chondrolectin (chodl) was isolated out of the tail tip of four-day old 129/SvJ mice as a by-product of a PCR-based subtractive cDNA library screening. The gene is predominantly expressed in adult skeletal muscle, heart, testes and lungs and in embryonic stadia. Chodl is the mouse homologue of human chondrolectin (CHODL), a gene that encodes for a type Ia transmembrane protein and that is expressed in human testis, prostate, heart and skeletal muscle tissue. CHODL-splice variants (CHODL ( f ), CHODL ( f Delta E ), CHODL ( Delta E )) are detected in human leukocytes. The proteins of the chondrolectin family belong to the family of C-type lectins. As the members of this protein family are important for a wide array of biological processes, the function of chodl was investigated by searching for its protein interaction partners. The beta-subunit of Rab geranylgeranyl transferase (Rabggtb) was isolated 8 times after a complete Sos recruitment system (SRS) screen with the cytoplasmic domain of chodl. The interaction was confirmed with in vitro transcription/translation and co-immunoprecipitation (co-IP) experiments.
Claessens
An
A
Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium.
Weyn
Christine
C
Merregaert
Joseph
J
D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D037181:Lectins, C-Type / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology; D014158:Transcription, Genetic
D037181:Lectins, C-Type; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; C474775:chondrolectin, mouse; D019883:Alkyl and Aryl Transferases; C075607:Rab geranylgeranyltransferase