Lipase-catalyzed synthesis of glucose fatty acid ester using ionic liquids mixtures.
Novozym 435-catalyzed synthesis of 6-O-lauroyl-d-glucose in ionic liquids (ILs) was investigated. The highest lipase activity was obtained in water-miscible [Bmim][TfO] which can dissolve high concentration of glucose, while the highest stability of lipase was shown in hydrophobic [Bmim][Tf(2)N]. The optimal activity and stability of lipase could be obtained in [Bmim][TfO] and [Bmim][Tf(2)N] mixture (1:1, v/v). Specifically, the activity of lipase was increased from 1.1 to 2.9 micromolmin(-1)g(-1) by using supersaturated glucose solution in this mixture, compared with reaction using saturated solution. After 5 times reuse of lipase, 86% of initial activity was remained in this mixture, while the residual activity in pure [Bmim][TfO] was 36%. Therefore, the productivity obtained by using ILs mixtures was higher than those in pure ILs.
Lee
Sang Hyun
SH
ERC for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Republic of Korea.
Ha
Sung Ho
SH
Hiep
Nguyen Minh
NM
Chang
Woo-Jin
WJ
Koo
Yoon-Mo
YM
D002384:Catalysis; D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids / Q000138:chemical synthesis / Q000737:chemistry*; D005656:Fungal Proteins; D005947:Glucose / Q000138:chemical synthesis / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D007848:Laurates / Q000737:chemistry; D008049:Lipase / Q000378:metabolism*
D004800:Enzymes, Immobilized; D004952:Esters; D005227:Fatty Acids; D005656:Fungal Proteins; D052578:Ionic Liquids; D007848:Laurates; C093667:Novozyme 435; D008049:Lipase; D005947:Glucose