title: Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo.
abstract: Our purpose is to evaluate intramucosal gastric pH video imaging by 2('),7(')-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied in 10 pigs: five in the control group and five with respiratory acidosis induced through rebreathing. The intramucosal pH of the gastric wall is measured every 5 s and the results demonstrate a good correlation (pearson correlation=0.832) between blood gases pH measurements and pH measured with the video endocopic imaging system. Our results confirm the feasibility of using BCECF fluorescence pH imaging to measure intramucosal pH in vivo.
authors.affiliation: Lille University Hospital, Pavillon Vancostenobel, 59037 Lille Cedex, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Rochon
authors.forename: Mercé
authors.initials: M
authors.lastname: Jourdain
authors.forename: Jacques
authors.initials: J
authors.lastname: Mangalaboyi
authors.forename: François
authors.initials: F
authors.lastname: Fourrier
authors.forename: Sylvie
authors.initials: S
authors.lastname: Soulié-Bégu
authors.forename: Bruno
authors.initials: B
authors.lastname: Buys
authors.forename: Guy
authors.initials: G
authors.lastname: Dehlin
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Lesage
authors.forename: Marie Christine
authors.initials: MC
authors.lastname: Chambrin
authors.forename: Serge
authors.initials: S
authors.lastname: Mordon
mesh_terms: D000142:Acidosis, Respiratory / Q000097:blood / Q000378:metabolism; D000818:Animals; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000098:blood supply / Q000378:metabolism*; D005773:Gastroscopy / Q000379:methods*; D006439:Hemodynamics; D006863:Hydrogen-Ion Concentration; D006935:Hypercapnia / Q000097:blood / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D034421:Sus scrofa
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D010100:Oxygen