[Contamination of IgG preparation for intravenous use with IgA. I. Development of ELISA tests for determination of low concentrations of IgAc, IgA1, IgA2, IgG and IgM].
Very low amounts of immunoglobulin contaminants present in preparations of immunoglobulin G for intravenous use (IVIG) require sophysticated procedures for detection of such low concentrations in milieu of comparatively very high level of IgG. Standard RID and nephelometry procedures are not adequate for these purposes. In process of removal of IgA contamination from IVIG, which is currently under development in Blood Transfusion Institute, Belgrade, it is necessary to have senzitive and very specific procedures for determination of and follow up of very low concentrations of IgA remauning in IVIG. We have established a highly senzitive and specific ELISA precedures for determination of very low concentrations of total IgA (IgAc), IgA1, IgA2, IgG and IgM. Commercial standards of IgAc, IgA1, IgA2, IgG and IgM were bound to PVC plates coated with corresponding monoclonal antibodies anti IgAc-IgA1, anti-IgG and anti-IgM. Resulting standard curves have shown high correlation coeficients with limited range of detection (two orders of magnitude in nanogram range). Very high specificity of ELISA tests were obtained due to the highly specific monoclonal antibodies used. Very high specificity and very low detection level of developed tests are advantageous compared to standard procedures for Ig concentration determination.
Dovezenski
N
N
Velicković
Z
Z
Tosić
Lj
Lj
Nikolić
G
G
Popović
O
O
Romić
M
M
Djokić
M
M
Mijusković
Z
Z
D004340:Drug Contamination; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D016756:Immunoglobulins, Intravenous / Q000737:chemistry*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D016756:Immunoglobulins, Intravenous