Headspace single-drop microextraction of herbal essential oils.
A method employing the headspace single-drop microextraction (HS-SDME) is presented for the determination of essential oils in dried herbal leaves. By optimising the key experimental parameters, a linear response for the individual target compounds was obtained in the concentration range from LOQ to 4 mg/mL (r(2) = 0.9912-0.9998), with LODs from 3.3 up to 20.5 microg per 100 g of dried leaves, and the repeatability within the RSD of 2.1-8.9%. The HS-SDME-based procedure, enabling a rapid and simple analysis of essential oils in herbs, was applied to selected real samples (nine essential oils in four different samples) in combination with GC-FID identification and quantification of the target volatiles.
Adam
Martin
M
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic. martin.adam@upce.cz
Dobiás
Petr
P
Eisner
Ales
A
Ventura
Karel
K
D002849:Chromatography, Gas / Q000379:methods*; D009822:Oils, Volatile / Q000032:analysis*; D013696:Temperature
D009822:Oils, Volatile