title: [Quantitative analysis of adenosine and cordycepin in Cordyceps sinensis and its substitutes with LC-MS-MS].
abstract: OBJECTIVE
  To develop a LC-MS-MS method for determination of adenosine and cordycepin in Cordyceps sinensis and it's substitutes.
  
  
  METHODS
  The sawple was extracted with. 90% methanol. Multi-reactions monitoring (MRM) technique was adopted.
  
  
  RESULTS
  The regression equations and coefficients were Y = 89.04X + 506.85 (r = 0.999 7) for adenosine, Y = 99.66X + 1 251.34 (r = 0.998 8) for cordycepin respectively. The linear range was 5.0-1 000.0 microg x L(-1) for adenosine and cordycepin. The limits of detection (LOD) were 0. 44 microg x L(-1) for adenosine and 0.31 microg x L(-1) for cordycepin, respectively. The average recoveries of adenosine and cordycepin were 98.1% and 97.9%, respectively.
  
  
  CONCLUSIONS
  The method was highly sensitive, selective and fast, which can be used for the determination of adenosine and cordycepin in C. sinensis and it's substitutes. This method can also be applied for the quality control of the medicinal materials.
authors.affiliation: Qingdao Institute for Drug Control, Qingdao 266071, China. yangzhao@yahoo.com.cn
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.forename: Shao-Yun
authors.initials: SY
authors.lastname: Chi
authors.forename: Chun-Hui
authors.initials: CH
authors.lastname: Zhang
authors.forename: Aiying
authors.initials: A
authors.lastname: Wu
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000592:standards; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry* / Q000145:classification; D003839:Deoxyadenosines / Q000032:analysis* / Q000592:standards; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003839:Deoxyadenosines; C058120:cordycepin; D000241:Adenosine