title: Novel multiplex PCR assay for simultaneous identification of community-associated methicillin-resistant Staphylococcus aureus strains USA300 and USA400 and detection of mecA and Panton-Valentine leukocidin genes, with discrimination of Staphylococcus aureus from coagulase-negative staphylococci.
abstract: We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 and USA400 strains and concomitant detection of Panton-Valentine leukocidin genes, with simultaneous discrimination of methicillin-resistant Staphylococcus aureus strains from methicillin-susceptible S. aureus strains, S. aureus strains from coagulase-negative staphylococci, and staphylococci from other bacteria.
authors.affiliation: Centre for Antimicrobial Resistance, Calgary Health Region, Calgary, Alberta, Canada. kzhang@ucalgary.ca
authors.forename: Kunyan
authors.initials: K
authors.lastname: Zhang
authors.forename: Jo-Ann
authors.initials: JA
authors.lastname: McClure
authors.forename: Sameer
authors.initials: S
authors.lastname: Elsayed
authors.forename: Thomas
authors.initials: T
authors.lastname: Louie
authors.forename: John M
authors.initials: JM
authors.lastname: Conly
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001427:Bacterial Toxins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D003030:Coagulase / Q000378:metabolism*; D017714:Community-Acquired Infections / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005098:Exotoxins / Q000235:genetics*; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D003030:Coagulase; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D008712:Methicillin