Does the oxidative stress in chronic obstructive pulmonary disease cause thioredoxin/peroxiredoxin oxidation?
The thioredoxin/peroxiredoxin system comprises a redox-regulated antioxidant family in human lung; its significance, regulation, or oxidation has not been evaluated in smoking-related lung diseases. Here, we present the expression of the thioredoxin/peroxiredoxin system in lung biopsies from normal lung (n = 14), smokers (n = 21), and patients with chronic obstructive pulmonary disease (COPD, n = 38), and assess the possible inactivation/oxidation of this system by nonreducing Western blotting, two-dimensional gel electrophoresis, and mass spectrometry. Our study shows that the thiol status of the Trx/Prx-system can be modulated in vitro, but it appears to have high resistance against the oxidative stress in COPD.
Lehtonen
Siri T
ST
Department of Internal Medicine, University of Oulu and Oulu University Hospital, Oulu, Finland.
Ohlmeier
Steffen
S
Kaarteenaho-Wiik
Riitta
R
Harju
Terttu
T
Pääkkö
Paavo
P
Soini
Ylermi
Y
Kinnula
Vuokko L
VL
D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000378:metabolism*; D029424:Pulmonary Disease, Chronic Obstructive / Q000378:metabolism* / Q000473:pathology; D013879:Thioredoxins / Q000378:metabolism*
D013879:Thioredoxins; D054464:Peroxiredoxins