title: Meal-induced compositional changes in blood and saliva in persons with bulimia nervosa.
abstract: BACKGROUND
  Binge eating episodes in persons with bulimia nervosa may to some extent be a result of disturbed sensations of hunger and satiety. It has been hypothesized that abnormal appetite sensations may be due to bulimia nervosa-related alterations in the release of hormones that are known to be involved in the physiologic regulation of appetite and metabolism.
  
  
  OBJECTIVE
  The objective was to investigate whether circulating concentrations of the appetite-regulating peptides leptin and ghrelin and markers of metabolism (glucose and insulin) are different in persons with bulimia nervosa than in controls before and after intake of a meal and whether these changes may be reflected in saliva.
  
  
  METHODS
  Twenty women with bulimia nervosa and 20 age- and sex-matched healthy controls participated. After an overnight fast, the subjects ate a standardized carbohydrate-rich breakfast. Whole saliva and blood were collected, and visual analogue scales for hunger and satiety were completed once before and continuously for 5 h after the breakfast.
  
  
  RESULTS
  A lower pre- and postprandial whole saliva flow rate was found in subjects with bulimia nervosa, which might have been attributable to a concomitant intake of potentially xerogenic medication. Subjects with bulimia nervosa experienced reduced hunger, which could not be explained by pre- or postprandial alterations in circulating ghrelin, leptin, insulin, or glucose concentrations.
  
  
  CONCLUSIONS
  There were no apparent differences in the composition of blood and saliva between bulimia nervosa and control subjects, and meal-induced compositional changes in blood were not directly mirrored in saliva composition.
authors.affiliation: Department of Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. awd@odont.ku.dk
authors.forename: Anja W
authors.initials: AW
authors.lastname: Dynesen
authors.forename: Allan
authors.initials: A
authors.lastname: Bardow
authors.forename: Arne
authors.initials: A
authors.lastname: Astrup
authors.forename: Birgit
authors.initials: B
authors.lastname: Petersson
authors.forename: Jens J
authors.initials: JJ
authors.lastname: Holst
authors.forename: Birgitte
authors.initials: B
authors.lastname: Nauntofte
mesh_terms: D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D052018:Bulimia Nervosa / Q000097:blood* / Q000503:physiopathology*; D016022:Case-Control Studies; D004435:Eating / Q000502:physiology*; D005260:Female; D054439:Ghrelin / Q000032:analysis / Q000097:blood; D006801:Humans; D006815:Hunger / Q000502:physiology; D007328:Insulin / Q000032:analysis / Q000097:blood; D020738:Leptin / Q000032:analysis / Q000378:metabolism; D019518:Postprandial Period / Q000502:physiology; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D012528:Satiety Response / Q000502:physiology; D014987:Xerostomia / Q000453:epidemiology*
chemical_list: D001786:Blood Glucose; D054439:Ghrelin; D007328:Insulin; D020738:Leptin