title: Convective assembly of bacteria for surface-enhanced Raman scattering.
abstract: A sample preparation method based on convective assembly for "whole-microorganism" identification using surface-enhanced Raman scattering (SERS) is developed. With this technique, a uniform sample can easily be prepared with silver nanoparticles. During the deposition process, bacteria and nanoparticles are assembled to form a unique well-ordered structure with great reproducibility. The SERS spectra acquired from the samples prepared with this technique have better quality and improved reproducibility for SERS spectra obtained from the same sample and limited variation due to the consistent sample preparation. E. coli, a Gram-negative bacilli, and Staphylococcus cohnii, a Gram-positive coccus, are studied as model bacteria.
authors.affiliation: Yeditepe University, Faculty of Engineering and Architecture, Genetics and Bioengineering Department, Kayisdagi, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kahraman
authors.forename: M Müge
authors.initials: MM
authors.lastname: Yazici
authors.forename: Fikrettin
authors.initials: F
authors.lastname: Sahin
authors.forename: Mustafa
authors.initials: M
authors.lastname: Culha
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000648:ultrastructure*; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D020099:Coated Materials, Biocompatible; D003102:Colloids; D004926:Escherichia coli / Q000737:chemistry / Q000648:ultrastructure; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D012834:Silver; D013059:Spectrum Analysis, Raman; D013210:Staphylococcus / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D020099:Coated Materials, Biocompatible; D003102:Colloids; D012834:Silver