[Protection of polysaccharides-2b from mudan cortex of Paeonia suffruticosa andr on diabetic cataract in rats].
OBJECTIVE
To observe the the protective effection of polysaccharides-2b of mudan cortex of Paeonia suffruticosa andr (PSM2b) on diabetic cataract.


METHODS
The animal model of diabetic cataract in rats was induced by streptozotocin (STZ) and freund's adjuvant complete (CFA). The initial opacity occurrence time in lens was investigated with cranny lamp, and opacity degree of lens was compared too. The activities of glutathione peroxidase (GSH-pX), superoxide dismutase (SOD), catalase (CAT), the content of malonaldehyde (MDA) in serum and lens were detected. At the same time, the activities of Na(+) -K(+) -ATPase, the content of macromolecular weight protein and infusibility protein in lens were detected too.


RESULTS
The results examinated by cranny lamp showed that PSM2b could significantly postpone the occurrence and alleviate opacity degree of lens. Compared with model group, every treatment group of PSM2b could lower the level of MDA, high and middle dose groups could increase the levels of SOD, GSH-pX, CAT in serum and lens in evidence, and enhance the activity of Na(+) -K(+) -ATPase. These indexes present favorable positive correlation between dose and effect.


CONCLUSIONS
All these results demonstrated that PSM2b had apparently protective effection on diabetic cataract in rats.
Zhao
Gen-Hai
GH
Institute of Biochemistry and Microbiology Anhui University, Key-Laboratory of Research and Development of Chinese Medicine Anhui Province, Hefei 230039, China.
Shen
Ye-Shou
YS
Ma
Jin-Bao
JB
Li
Feng
F
Shi
Xin-Qiang
XQ
D000818:Animals; D002374:Catalase / Q000097:blood / Q000378:metabolism; D002386:Cataract / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000139:chemically induced / Q000150:complications*; D005979:Glutathione Peroxidase / Q000097:blood / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D029596:Paeonia / Q000737:chemistry*; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D011134:Polysaccharides; D008315:Malondialdehyde; D013311:Streptozocin; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000254:Sodium-Potassium-Exchanging ATPase