Structural elucidation of the polysaccharide moiety of a glycopeptide (GLPCW-II) from Ganoderma lucidum fruiting bodies.
A water-soluble glycopeptide (GLPCW-II) was isolated from the fruiting bodies of Ganoderma lucidum by DEAE-Sepharose Fast-Flow and Sephacryl S-300 High Resolution Chromatography. The glycopeptide had a molecular weight of 1.2x10(4)Da (determined by HPLC), and consisted of approximately 90% carbohydrate and approximately 8% protein as determined using the phenol-sulfuric acid method and the BCA protein assay reagent kit, respectively. The polysaccharide moiety was composed mainly of D-Glc, L-Fuc, and D-Gal in the ratio of 1.00:1.09:4.09. To facilitate structure-activity studies, the structure of the GLPCW-II polysaccharide moiety was elucidated using 1H and 13C NMR spectroscopy including COSY, TOCSY, HMBC, HSQC, and ROESY, combined with GC-MS of methylated derivatives, and shown to consist of repeating units with the following structure: [Formula: see text].
Ye
LiBin
L
College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
Zhang
JingSong
J
Ye
XiJun
X
Tang
QingJiu
Q
Liu
YanFang
Y
Gong
ChunYu
C
Du
XiuJui
X
Pan
YingJie
Y
D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D020940:Reishi / Q000737:chemistry*
D006020:Glycopeptides; D011134:Polysaccharides