title: Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate.
abstract: The most commonly used protocol of the RNA isolation, the guanidine thiocyanate method, was unsuitable for recalcitrant plant tissues containing a large amount of storage proteins and secondary metabolites. We demonstrated that RNA could bind to the silica particles, which have been used successfully in DNA isolation from various sources, under a high concentration of NaCl in the presence of ethanol and sodium acetate. Based on this observation, an efficient, inexpensive, and highly reproducible technique, the acid phenol-silica method, was developed to isolate high-quality RNAs from various plant tissues recalcitrant to extraction in guanidine thiocyanate.
authors.affiliation: Key Laboratory of Gene Engineering of the Ministry of Education and State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ling-Wen
authors.initials: LW
authors.lastname: Ding
authors.forename: Qiao-Yang
authors.initials: QY
authors.lastname: Sun
authors.forename: Zhao-Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Sun
authors.forename: Zeng-Fu
authors.initials: ZF
authors.lastname: Xu
mesh_terms: D004587:Electrophoresis, Agar Gel; D006146:Guanidines / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D010944:Plants / Q000737:chemistry* / Q000235:genetics; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000737:chemistry*; D013861:Thiocyanates / Q000737:chemistry*
chemical_list: D006146:Guanidines; D010936:Plant Extracts; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012313:RNA; D012822:Silicon Dioxide