Human bocavirus (HBoV) in Thailand: clinical manifestations in a hospitalized pediatric patient and molecular virus characterization.
OBJECTIVE
Human bocavirus (HBoV), a novel virus, which based on molecular analysis has been associated with respiratory tract diseases in infants and children have recently been studied worldwide. To determine prevalence, clinical features and perform phylogenetic analysis in HBoV infected Thai pediatric patients.


METHODS
HBoV was detected from 302 nasopharyngeal (NP) suctions of pediatric patients with acute lower respiratory tract illness and sequenced applying molecular techniques.


RESULTS
The incidence of HBoV infection in pediatric patients amounted to 6.62% with 40% co-infected with other respiratory viruses. There were no clinical specific manifestations for HBoV; however, fever and productive cough were commonly found. Generalized rales and wheezing were detected in most of the patients as well as perihilar infiltrates. The alignment and phylogenetic analysis of partial VP1 genes showed minor variations.


CONCLUSIONS
Our results indicated that HBoV can be detected in nasopharyngeal aspirate specimens from infants and children with acute lower respiratory tract illness.
Chieochansin
Thaweesak
T
Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Samransamruajkit
Rujipat
R
Chutinimitkul
Salin
S
Payungporn
Sunchai
S
Hiranras
Thitikul
T
Theamboonlers
Apiradee
A
Poovorawan
Yong
Y
D000293:Adolescent; D052660:Bocavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D005260:Female; D006760:Hospitalization; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000503:physiopathology / Q000821:virology; D048348:Reverse Transcription; D017422:Sequence Analysis, DNA; D013785:Thailand / Q000453:epidemiology
D036022:Capsid Proteins