title: Application of multibounce attenuated total reflectance fourier transform infrared spectroscopy and chemometrics for determination of aspartame in soft drinks.
abstract: Aspartame is a low-calorie sweetener commonly used in soft drinks; however, the maximum usage dose is limited by the U.S. Food and Drug Administration. Fourier transform infrared (FTIR) spectroscopy with attenuated total reflectance sampling accessory and partial least-squares regression (PLS) was used for rapid determination of aspartame in soft drinks. On the basis of spectral characterization, the highest R2 value, and lowest PRESS value, the spectral region between 1600 and 1900 cm(-1) was selected for quantitative estimation of aspartame. The potential of FTIR spectroscopy for aspartame quantification was examined and validated by the conventional HPLC method. Using the FTIR method, aspartame contents in four selected carbonated diet soft drinks were found to average from 0.43 to 0.50 mg/mL with prediction errors ranging from 2.4 to 5.7% when compared with HPLC measurements. The developed method also showed a high degree of accuracy because real samples were used for calibration, thus minimizing potential interference errors. The FTIR method developed can be suitably used for routine quality control analysis of aspartame in the beverage-manufacturing sector.
authors.affiliation: Department of Human Nutrition, Food and Animal Science and Department of Molecular Biosciences and Bioengineering, University of Hawaii, Honolulu, Hawaii 96822, USA.
authors.forename: Harpreet Kaur
authors.initials: HK
authors.lastname: Khurana
authors.forename: Il Kyu
authors.initials: IK
authors.lastname: Cho
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Shim
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
authors.forename: Soojin
authors.initials: S
authors.lastname: Jun
mesh_terms: D001218:Aspartame / Q000032:analysis*; D002253:Carbonated Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D016018:Least-Squares Analysis; D011786:Quality Control; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D001218:Aspartame