title: Changes in stiffness of demineralized dentin following application of collagen crosslinkers.
abstract: It is thought that increasing the strength of the dentin matrix using crosslinking agents may improve both the strength and the durability of resin-dentin bonds. The purpose of this study was to evaluate the effect of two collagen crosslinking agents (glutaraldehyde, GD and grape seed extract, GSE) on the modulus of elasticity of demineralized dentin. Sound molar fragments were fully demineralized and divided into five groups according to the type and concentration of crosslinking agents: 2.5% GD; 5% GD, 25% GD; 0.65% GSE; 6.5% GSE. Specimens were immersed in their respective solution and tested at baseline, 10 min, 30 min, 1 h, 2 h, 4 h. The elastic modulus of dentin was significantly affected by the treatment (p < 0.01) and exposure time (p < 0.01). There was a statistically significant interaction between the two factors evaluated (treatment vs. time p < 0.01). Mean baselines values varied between 4.8 and 6.2 MPa in water; after 4 h of treatment the values increased between 34.9 and 242.5 MPa, that were treatment time and agent dependent. The use of these collagen crosslinkers to increase the stiffness of demineralized dentin, was both concentration and time dependent.
authors.affiliation: Department of Restorative Dentistry, University of Illinois at Chicago, College of Dentistry, Chicago, Illinois, USA. bedran@uic.edu
authors.forename: Ana Karina B
authors.initials: AK
authors.lastname: Bedran-Russo
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Kelli
authors.initials: K
authors.lastname: Agee
authors.forename: James L
authors.initials: JL
authors.lastname: Drummond
authors.forename: Klaus J
authors.initials: KJ
authors.lastname: Miescke
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000737:chemistry*; D004548:Elasticity; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D010936:Plant Extracts / Q000737:chemistry; D027843:Vitis / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D010936:Plant Extracts; D005976:Glutaral