title: Serotypes A1 and A2 of Mannheimia haemolytica are susceptible to genotypic, capsular and phenotypic variations in contrast to T3 and T4 serotypes of Bibersteinia (Pasteurella) trehalosi.
abstract: Mannheimia haemolytica and Bibersteinia (Pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. The disease is determined by specific serotypes found in cattle and small ruminants. The molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. This study aimed to detect the instability and variations of bacterial strains that may affect the analysis of epidemic strains, or the stability of vaccinal strains. Eight strains of M. haemolytica belonging to serotypes A1 and A2 and three B. trehalosi strains of the T3 and T4 serotypes were used. Strains were subjected to pulsed field gel electrophoresis (PFGE) and capsular and phenotypic typing at each round of a total of 50 successive subcultures. Remarkable stability was found in all selected strains of B. trehalosi in contrast to M. haemoltyica, in which strains of both serotypes showed pattern variations produced by PFGE and capsular and phenotypic analysis. Objective criteria for M. haemolytica and B. trehalosi typing are consequently addressed.
authors.affiliation: Research Group on Bacterial Opportunistic Pathogens and Environment, Université de Lyon, Lyon, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Villard
authors.forename: Dominique
authors.initials: D
authors.lastname: Gauthier
authors.forename: Françoise
authors.initials: F
authors.lastname: Maurin
authors.forename: Evelyne
authors.initials: E
authors.lastname: Borges
authors.forename: Yves
authors.initials: Y
authors.lastname: Richard
authors.forename: Georgette
authors.initials: G
authors.lastname: Abadie
authors.forename: Angeli
authors.initials: A
authors.lastname: Kodjo
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006386:Hemagglutination Tests; D016978:Mannheimia haemolytica / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010325:Pasteurella / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D012703:Serotyping
chemical_list: D004269:DNA, Bacterial