title: Fluorescence quenching method for the determination of bleomycins A5 and A2 with halofluorescein dyes.
abstract: In weak acidic medium, the anticancer antibiotics bleomycin A5 (BLMA5) and bleomycin A2 (BLMA2) bind with halofluorescein dyes, such as erythrosin (Ery), eosin Y (EY) and eosin B (EB), to form ion-association complexes, which causes fluorescence quenching of halofluorescein dyes. The quenching values (DeltaF) are directly in proportional to the concentrations of bleomycins over the range 0.09-2.5 microg/mL. Based on this, a fluorescence quenching method for the determination of BLMA5 and BLMA2 has been developed. The dynamic range is 0.12-2.5 microg/mL for the determination of BLMA5 and 0.09-2.0 microg/mL for BLMA2, with detection limits (3sigma) of 0.04 microg/mL for BLMA5, 0.03 microg/mL for BLMA2, respectively. It has been applied to determine the two antibiotics in human serum, urine and rabbit serum samples. The recovery is in the range 90-102%. In this work, the optimum reaction conditions and the spectral characteristics of the fluorescence are investigated. The reasons for fluorescence quenching are discussed, based on the fluorescence theory.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Beibei, People's Republic of China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Liu
authors.forename: Zhongfang
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Hu
authors.forename: Ling
authors.initials: L
authors.lastname: Kong
authors.forename: Shaopu
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D011817:Rabbits; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D001761:Bleomycin; C025703:bleomycetin