[Transformation of full-length gene of mouse/human chimeric antibody against Hantaan virus into Arabidopsis thaliana].
OBJECTIVE
To construct the transgenic Arabidopsis thaliana containing full-length gene of mouse/human chimeric antibody(3G1MH) against Hantaan virus.


METHODS
The recombinant plasmid 3G1MH-pCAMBIA2301 was transformed into Agrobacterium tumefaciens GV3101 by TSS freeze-thaw method, and then the recombinant was transferred into wild Arabidopsis thaliana by vacuum-transgenic method. The regenerated transgenic plants were selected with kanamycin, and confirmed by PCR and Northern blot.


RESULTS
PCR result showed stable integration of the 3G1MH gene IN Arabidopsis thaliana genome in 7 stains of the transformed plants. Northern blot analyses confirmed the transcription of heavy and light chains in the transgenic plants.


CONCLUSIONS
The successful establishment of 3G1MH transgenic Arabidopsis thaliana plants pave the way for further research on expressing therapeutic antibody in transgenic plants.
Zhou
Wei
W
Department of Microbiology, Fourth Military Medical University, Xi'an 710032, China.
Wu
Xing-an
XA
Luo
Wen
W
Hu
Gang
G
Zhang
Fang-lin
FL
Bai
Wen-tao
WT
Zhang
Liang
L
Yu
Lan
L
Shi
Meng-yuan
MY
Xu
Zhi-kai
ZK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000276:immunology; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000276:immunology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D029681:Arabidopsis Proteins; D011994:Recombinant Proteins