Single-shot detection of bacterial endospores via coherent Raman spectroscopy.
Recent advances in coherent Raman spectroscopy hold exciting promise for many potential applications. For example, a technique, mitigating the nonresonant four-wave-mixing noise while maximizing the Raman-resonant signal, has been developed and applied to the problem of real-time detection of bacterial endospores. After a brief review of the technique essentials, we show how extensions of our earlier experimental work [Pestov D, et al. (2007) Science 316:265-268] yield single-shot identification of a small sample of Bacillus subtilis endospores (approximately 10(4) spores). The results convey the utility of the technique and its potential for "on-the-fly" detection of biohazards, such as Bacillus anthracis. The application of optimized coherent anti-Stokes Raman scattering scheme to problems requiring chemical specificity and short signal acquisition times is demonstrated.
Pestov
Dmitry
D
Institute for Quantum Studies and Department of Physics, Texas A&M University, College Station, TX 77843, USA. dmip@neo.tamu.edu
Wang
Xi
X
Ariunbold
Gombojav O
GO
Murawski
Robert K
RK
Sautenkov
Vladimir A
VA
Dogariu
Arthur
A
Sokolov
Alexei V
AV
Scully
Marlan O
MO
D001412:Bacillus subtilis / Q000378:metabolism*; D001694:Biological Warfare; D001709:Biotechnology / Q000379:methods; D023482:Bioterrorism; D015964:Gene Expression Regulation, Bacterial; D015386:Hazardous Substances; D007259:Infrared Rays; D008853:Microscopy / Q000379:methods; D015233:Models, Statistical; D016011:Normal Distribution; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013171:Spores, Bacterial / Q000737:chemistry*
D015386:Hazardous Substances