title: Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera).
abstract: A Gram-positive, rod-shaped or coccoid, yellow-pigmented bacterial strain, D287(T), was isolated from the water flea Daphnia cucullata (Crustacea: Cladocera) collected from Lake Balaton in Hungary. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparisons revealed that the strain represented a distinct lineage within the cluster of the genera Nocardioides and Marmoricola. The following characteristics were consistent with the affiliation of strain D287(T) to the genus Nocardioides: peptidoglycan based on LL-2,6-diaminopimelic acid, MK-8(H(4)) as the major menaquinone, iso-C(16:0) as the predominant cellular fatty acid, the presence of phosphatidylglycerol and diphosphatidylglycerol and a DNA G+C content of 69.9 mol%. Owing to characteristic differences in physiological traits and levels of 16S rRNA gene sequence similarity to its phylogenetically closest neighbours that were below 97%, strain D287(T) is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioides daphniae sp. nov. is proposed. The type strain is D287(T) (=DSM 18664(T)=CCM 7403(T)).
authors.affiliation: Eötvös Loránd University, Faculty of Science, Department of Microbiology, Pázmány Péter sétány 1/c, H-1117 Budapest, Hungary. totherika@ludens.elte.hu
authors.forename: Erika M
authors.initials: EM
authors.lastname: Tóth
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Kéki
authors.forename: Zalán G
authors.initials: ZG
authors.lastname: Homonnay
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Borsodi
authors.forename: Károly
authors.initials: K
authors.lastname: Márialigeti
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D003621:Daphnia / Q000382:microbiology*; D020459:Genes, rRNA; D006814:Hungary; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S