[Antinuclear antibodies detection in both HEp-2 cells and tissue section of the rat liver using indirect imunofluorescence].
Having in mind the importance of indirect immunofluorescence in antinuclear antibodies detection, the study was aimed to compare characteristics of fluorescent visualization and specificity of HEp-2 cells with tissue cryosections of the rat liver. The study included 76 serum samples obtained from patients with systemic connective tissue diseases. Out of that number, fluorescence findings on HEp-2 cells and rat liver tissue were positive in 74 and 54 patients, respectively. At the same time, only foyr basic types of fluorescence were manifest in tissue samples, while HEp-2 cells revealed 3 mixed types, and within the patchy type, several subgroups were evidenced. Out of the 20 serum samples which enabled positive results only with HEp-2 cells, they all belonged to the group of patchy fluorescence. In addition to the better fluorescence visualization, owing to its more complexs antigenic structure. HEp-2 cells revealed significantly higher specificity when compared to the rat liver tissue thus enabling definitive identification of sertain specific autoantibodies, or they were used as screening methods for its further detection.
Balaban
J
J
Tanurdzić
S
S
Mircetić
V
V
Petrović
M
M
Damjanov
N
N
Janosević
S
S
Nikolov
V
V
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D002460:Cell Line; D003240:Connective Tissue Diseases / Q000276:immunology; D004848:Epithelium / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008099:Liver / Q000276:immunology*; D051381:Rats
D000974:Antibodies, Antinuclear