Evaluation of the platelet counting by Abbott CELL-DYN SAPPHIRE haematology analyser compared with flow cytometry.
The Abbot Cell-Dyn Sapphire is a new generation haematology analyser. The system uses optical/fluorescence flow cytometry in combination with electronic impedance to produce a full blood count. Optical and impedance are the default methods for platelet counting while automated CD61-immunoplatelet analysis can be run as selectable test. The aim of this study was to determine the platelet count performance of the three counting methods available on the instrument and to compare the results with those provided by Becton Dickinson FACSCalibur flow cytometer used as reference method. A lipid interference experiment was also performed. Linearity, carryover and precision were good, and satisfactory agreement with reference method was found for the impedance, optical and CD61-immunoplatelet analysis, although this latter provided the closest results in comparison with flow cytometry. In the lipid interference experiment, a moderate inaccuracy of optical and immunoplatelet counts was observed starting from a very high lipid value.
Grimaldi
E
E
Department of Biochemistry and Medical Biotechnology, University Federico II, Naples, Italy. ermalu.mare@libero.it
Del Vecchio
L
L
Scopacasa
F
F
Lo Pardo
C
C
Capone
F
F
Pariante
S
S
Scalia
G
G
De Caterina
M
M
D005434:Flow Cytometry / Q000295:instrumentation; D006801:Humans; D039661:Integrin beta3 / Q000032:analysis*; D010976:Platelet Count / Q000295:instrumentation*
C061248:ITGB3 protein, human; D039661:Integrin beta3