EID-1 interacts with orphan nuclear receptor SF-1 and represses its transactivation.
The orphan nuclear receptor, SF-1, plays a pivotal role in the development and differentiation of the endocrine and reproductive systems, and also regulates the transcription of a host of genes, including those encoding several steroidogenic enzymes and gonadotropins. We found that a previously unidentified repressor, EID-1, is an SF-1-interacting protein that inhibits the transactivation of SF-1. A transient transfection assay revealed that EID-1 inhibits SF-1, but not LRH-1, ERRgamma, or mCAR. Using the yeast two hybrid and GST pull-down assays, we determined that EID-1 interacted strongly with SF-1. In addition, it colocalized with SF-1 in mammalian cells and interacted specifically with the AF-2 domain of SF-1, competing with SRC-1 to inhibit SF-1 transactivation. EID-1 is expressed in the mouse testis, and its expression decreases during testis development. The results of the present study suggest that EID-1 can act as a repressor, regulating the function of SF-1.
Park
Yun-Yong
YY
Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea.
Park
Ki Cheol
KC
Shong
Minho
M
Lee
Soon-Jung
SJ
Lee
Young-Ho
YH
Choi
Hueng-Sik
HS
D000818:Animals; D019556:COS Cells; D018797:Cell Cycle Proteins; D002467:Cell Nucleus / Q000737:chemistry; D002522:Chlorocebus aethiops; D006801:Humans; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism* / Q000494:pharmacology; D012097:Repressor Proteins / Q000378:metabolism* / Q000494:pharmacology; D054339:Steroidogenic Factor 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013737:Testis / Q000254:growth & development / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D018797:Cell Cycle Proteins; C416925:EID1 protein, human; D009687:Nuclear Proteins; D012097:Repressor Proteins; D054339:Steroidogenic Factor 1