title: Monocyte chemoattractant protein-1 immunoreactivity in sensory ganglia and hindpaw after adjuvant injection.
abstract: Monocyte chemoattractant protein-1 (MCP-1)/CCL2 is a member of the CC chemokine family that exhibits potent chemotactic activity for monocytes/macrophages. In the current study, the proportion of monocyte chemoattractant protein-1-immunoreactive (IR) neurons in the dorsal root ganglion (DRG) of rats was shown to increase markedly following adjuvant injection into the hindpaw. MCP-1-IR axon terminals were not found in the spinal cord or hindpaw of control or adjuvant-treated rats. Instead, the inflamed hindpaw dermis was infiltrated by numerous MCP-1-IR inflammatory cells. Following adjuvant injection, the majority of MCP-1-IR neurons in the DRG colocalized with IB4 binding. Our findings suggest that peripheral tissue inflammation induces increased MCP-1 expression in DRG neurons and this may be dependent upon glial cell line-derived neurotrophic factor.
authors.affiliation: Department of Anatomy, School of Medicine, Kyungpook National University cDepartment of Anesthesiology and Pain Medicine, Kyungpook National University Hospital, Daegu, South Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Jeon
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Eun-Sung
authors.initials: ES
authors.lastname: Park
authors.forename: Young-Hoon
authors.initials: YH
authors.lastname: Jeon
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Cho
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000276:immunology / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology*; D005620:Freund's Adjuvant; D005727:Ganglia, Spinal / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000276:immunology / Q000378:metabolism; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology; D007249:Inflammation / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D018836:Inflammation Mediators; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D009475:Neurons, Afferent / Q000276:immunology / Q000378:metabolism*; D010523:Peripheral Nervous System Diseases / Q000276:immunology / Q000378:metabolism* / Q000503:physiopathology; D037121:Plant Lectins; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000276:immunology / Q000294:innervation / Q000503:physiopathology; D015854:Up-Regulation / Q000276:immunology
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D051100:Glial Cell Line-Derived Neurotrophic Factor; C045475:Griffonia simplicifolia lectins; D018836:Inflammation Mediators; D037121:Plant Lectins; D005620:Freund's Adjuvant