Sensitive determination of ranitidine in rabbit plasma by HPLC with fluorescence detection.
A sensitive high-performance liquid chromatographic method for determination of ranitidine (RAN) in rabbit plasma is described. The method is based on liquid-liquid extraction, labeling with dansyl chloride and monitoring with fluorescence detector at 338nm (ex)/523nm (em). Plasma samples were extracted with diethyl ether alkalinized with 1M sodium hydroxide. Ephedrine HCl (EPH-HCl) was used as internal standard. Both, RAN and EPH were completely derivatized after heating at 60 degrees C for 10min in sodium bicarbonate solution (pH 9.5). The derivatized samples were analyzed by HPLC using Agilent Zorbax Extended C18 column (150mmx4.6mm i.d.) and mobile phase consists of 48% acetonitrile and 52% sodium acetate solution (0.02M, pH 4.6). The linearity of the method was in the range of 0.025-10microg/ml. The limits of detection (LOD) and quantification (LOQ) were 7.5+/-0.18 and 22.5+/-0.12ng/ml, respectively. Ranitidine recovery was 97.5+/-1.1% (n=6; R.S.D.=1.8%). The method was applied on plasma collected from rabbits at different time intervals after oral administration of 5mg/kg ranitidine HCl.
Khedr
Alaa
A
Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdulaziz University, Jamea Street, Jeddah 21589, Saudi Arabia. akhedr@kau.edu.sa
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006635:Histamine H2 Antagonists / Q000097:blood*; D008297:Male; D011817:Rabbits; D011899:Ranitidine / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
D006635:Histamine H2 Antagonists; D011899:Ranitidine