title: Flow cytometric measurement of ABO antibodies in ABO-incompatible living donor kidney transplantation.
abstract: Due to different detection methods, a comparison of anti-A/B antibody (Ab) levels among transplantation centers after living donor ABO-incompatible kidney transplantation is problematic. In the present study, anti-A/B Ab levels were determined prior to, and after, blood group A-to-O kidney transplantation using a recently established semiquantitative flow cytometry-based method, ABO fluorescence-activated cell sorting (ABO-FACS), and compared with standard agglutination titers and indirect antiglobulin testing. Pretransplant agglutination titers were reduced from 1:64 to 1:4, by a total of 14 Glycosorb A column immunoadsorptions (IADSs). Compared with the agglutination titers, antidonor immunoglobulin (Ig) M ABO-FACS mean fluorescence intensity ratios (MFIRs) decreased faster and remained low. No difference was observed using donor type or third-party A red blood cells (RBCs) for the ABO-FACS. Glycosorb A columns were not specific, also reducing anti-B and antiporcine IgM levels, which was confirmed by detecting anti-A/B and antiporcine Abs in the column eluates. In conclusion, analysis of pre- and posttransplant Abs from ABO-incompatible kidney transplant recipients by ABO-FACS allows a better understanding of Ab kinetics, which may improve the design of future IADS protocols.
authors.affiliation: Laboratory for Transplantation Immunology, University Hospital Zurich, Zurich, Switzerland.
authors.forename: Gisella Puga
authors.initials: GP
authors.lastname: Yung
authors.forename: Piero V
authors.initials: PV
authors.lastname: Valli
authors.forename: Astrid
authors.initials: A
authors.lastname: Starke
authors.forename: Regula J
authors.initials: RJ
authors.lastname: Mueller
authors.forename: Thomas
authors.initials: T
authors.lastname: Fehr
authors.forename: Marija
authors.initials: M
authors.lastname: Cesar-Ozpamir
authors.forename: Urs
authors.initials: U
authors.lastname: Schanz
authors.forename: Markus
authors.initials: M
authors.lastname: Weber
authors.forename: Rudolf P
authors.initials: RP
authors.lastname: Wüthrich
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Seebach
authors.forename: Georg
authors.initials: G
authors.lastname: Stussi
mesh_terms: D000017:ABO Blood-Group System; D000327:Adsorption; D000371:Agglutination; D000818:Animals; D001787:Blood Group Incompatibility; D002469:Cell Separation; D004912:Erythrocytes; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000737:chemistry; D016030:Kidney Transplantation / Q000379:methods*; D007700:Kinetics; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D013552:Swine
chemical_list: D000017:ABO Blood-Group System; D007075:Immunoglobulin M