title: [Effect of Coriolus versicolor polysaccharide B on membrane glycosaminoglycans and cellular glutathione changes in RAW264.7 macrophages exposed to angiotensin II].
abstract: OBJECTIVE
  To investigate the effect of Coriolus versicolor polysaccharide B (CVP-B) on increased membrane glycosaminoglycans (GAG) expression and intracellular glutathione (GSH) of RAW264.7 macrophages exposed to angiotensin II (Ang II).
  
  
  METHODS
  The plasma membrane of RAW264.7 macrophages exposed to Ang II treatment was isolated by ultracentrifugation, and the membrane GAG expression was analyzed using 1, 9-dimethylmethylene blue (DMMB) spectrophotometric assay for sulfated GAG. The intracellular reduced GSH was determined using fluorophotometry.
  
  
  RESULTS
  The GAG content in the macrophage membranes increased by up to 54% following cell exposure to 1.0 micromol/L Ang II, whereas in presence of 1.0 micromol;/L Ang II, CVP-B at 1, 10, and 50 microg/ml decreased the GAG content by 13%, 43% (P<0.01), and 52% (P<0.01), respectively. The macrophage GSH activity decreased by 69% following incubation with 1.0 micromol;/L Ang II for 24 h, and CVP-B treatment at 1, 10, and 50 microg/ml in presence of 1.0 micromol;/L Ang II resulted in significant increment of GSH activity by 31%(P<0.05), 104% (P<0.01), and 168% (P<0.01), respectively.
  
  
  CONCLUSIONS
  These data provide the first evidence that CVP-B inhibits elevated GAG expression in RAW264.7 macrophage membrane induced by Ang II.
authors.affiliation: Intensive Care Unit, Sun Yat-sen University Cancer Center, Guangzhou 510060, China. louning@mail.sysu.edu.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Lou
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.forename: Dao-feng
authors.initials: DF
authors.lastname: Wang
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Zhu
authors.forename: Quan-guan
authors.initials: QG
authors.lastname: Su
authors.forename: Yi
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D005978:Glutathione / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D006025:Glycosaminoglycans; D011134:Polysaccharides; D000804:Angiotensin II; D005978:Glutathione