title: Physiological up-regulation of inhibitory receptors Fc gamma RII and CR1 on memory B cells is lacking in SLE patients.
abstract: Under physiological conditions immune complexes (IC) are efficiently cleared from the circulation and meanwhile provide important feedback signals for the immune system via Fc gamma Rs and complement receptors. Dysregulation of these mechanisms have been implicated in conditions where IC concentrations reach pathological levels and inflict diseases, like systemic lupus erythematosus (SLE). Our aim was to compare distinct sub-populations of CD19(+) B cells of healthy individuals and SLE patients with regard to their expression of Fc gamma R type II (Fc gamma RII, CD32), complement receptor type 1 (CR1, CD35) and complement receptor type 2 (CR2, CD21) and sIgG/IgM. The following four groups of peripheral CD19(+) B cells were investigated: IgM(+)/CD27(-) naive, IgM(+)/CD27(+) and IgM(-)/CD27(+) memory cells and CD27(high) plasmablasts. We demonstrate that the expression of the inhibitory receptors Fc gamma RII and CR1 is up-regulated on peripheral memory B cells of healthy controls, whereas this up-regulation is considerably impaired on the memory B cells of SLE patients. This reduction affects both the IgM(+) and switched memory B cells. We found a striking difference between the expression of complement receptors CD21 and CD35; namely, no up-regulation of CD21 occurred on the memory B cells of healthy donors, and its decreased expression in SLE patients was characteristic for both the CD27(-) naive and the CD27(+) memory B-cell populations. Our results clearly demonstrate that the previously reported reduced expression of IC-binding receptors is mainly due to the disturbed memory compartment; however, the higher frequency of CD19(+)/CD27(high)/sIg(low) plasmablasts expressing minimal levels of these receptors also contributes to this diminution.
authors.affiliation: Research Group of the Hungarian Academy of Sciences, Eötvös Loránd University, Pázmány Péter s. 1/C, Budapest H-1117, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Isaák
authors.forename: Péter
authors.initials: P
authors.lastname: Gergely
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Szekeres
authors.forename: József
authors.initials: J
authors.lastname: Prechl
authors.forename: Gyula
authors.initials: G
authors.lastname: Poór
authors.forename: Anna
authors.initials: A
authors.lastname: Erdei
authors.forename: János
authors.initials: J
authors.lastname: Gergely
mesh_terms: D018941:Antigens, CD19 / Q000378:metabolism; D015551:Autoimmunity; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D007156:Immunologic Memory; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000503:physiopathology; D011951:Receptors, Complement / Q000378:metabolism*; D017464:Receptors, Complement 3d / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018941:Antigens, CD19; D007075:Immunoglobulin M; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D017452:Receptors, IgG; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7