A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.
An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels directly from cell culture media in contrast to previous reports that required extensive purification and proteolytic digestion. The sulfation site was not previously described nor predicted by informatic analysis of the peptide's precursor sequence.
Taylor
Steven W
SW
Amylin Pharmaceuticals, Inc., 9360 Towne Centre Drive, San Diego, California 92121, USA. staylor@amylin.com
Sun
Chengzao
C
Hsieh
Amy
A
Andon
Nancy L
NL
Ghosh
Soumitra S
SS
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D053378:Chromogranin B / Q000737:chemistry* / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D004285:Dogs; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000378:metabolism; D010748:Phosphopeptides / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000737:chemistry; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013463:Sulfuric Acid Esters / Q000737:chemistry* / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000235:genetics
D053378:Chromogranin B; D017077:Culture Media, Conditioned; D009479:Neuropeptides; D010748:Phosphopeptides; D013463:Sulfuric Acid Esters; C529325:chrombacin h peptide, rat; D010768:Phosphoserine; C005662:tyrosine O-sulfate; D014443:Tyrosine