URB is abundantly expressed in adipose tissue and dysregulated in obesity.
Dysregulated production of adipocytokines in obesity is involved in the development of metabolic syndrome. URB/DRO1 contains N-terminal signal sequence and is thought to play a role in apoptosis of tumor cells. In the present study, we investigated the expression pattern of URB mRNA in adipose tissue and secretion from cultured adipocytes. In human and mouse, URB mRNA was predominantly expressed in adipose tissue and was downregulated in obese mouse models, such as ob/ob, KKAy, and diet-induced obese mice. In 3T3L1 adipocytes, insulin, TNF-alpha, H(2)O(2) and hypoxia decreased URB mRNA level. This regulation was similar to that for adiponectin and opposite to MCP-1. URB protein was secreted in media of URB cDNA-stably transfected cells and endogenous URB was detected in media of cultured human adipocytes. In conclusion, the expression pattern of URB suggests its role in obesity and the results suggest that URB is secreted, at least in part, from adipocytes.
Okada
Takuya
T
Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamadaoka, Suita 565-0871, Japan.
Nishizawa
Hitoshi
H
Kurata
Akifumi
A
Tamba
Sachiko
S
Sonoda
Mina
M
Yasui
Atsutaka
A
Kuroda
Yohei
Y
Hibuse
Toshiyuki
T
Maeda
Norikazu
N
Kihara
Shinji
S
Hadama
Tohru
T
Tobita
Kazuki
K
Akamatsu
Suguru
S
Maeda
Kazuhisa
K
Shimomura
Iichiro
I
Funahashi
Tohru
T
D041721:3T3-L1 Cells; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D015536:Down-Regulation; D016326:Extracellular Matrix Proteins; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism*; D009928:Organ Specificity; D013045:Species Specificity; D014018:Tissue Distribution; D025521:Tumor Suppressor Proteins
C492631:CCDC80 protein, human; C448780:Ccdc80 protein, mouse; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D025521:Tumor Suppressor Proteins