[Expression of lymphotactin in renal tuberculosis].
OBJECTIVE
To study the expression of lymphotactin in normal kidney and renal tuberculosis and the arrangement of lymphotactin and infiltrating CD4(+) T cells and CD8(+) T cells in renal tuberculosis.


METHODS
HLptn cDNA of tissues from six cases with normal kidneys and ten cases with tuberculous kidneys was amplified by RT-PCR. The RT-PCR products were separated with 2% of gel. The cDNA was cloned to vector pGM-T Easy and was completely sequenced. The immunohistochemical staining method was used to examine the expression of lymphotactin in normal kidneys and tuberculous kidneys and the expression of CD4 and CD8 in tuberculous kidneys.


RESULTS
The sequence of cloned hLptn cDNA was confirmed and it was identical with the sequence of NO.U23772 published in GenBank. Both normal kidneys and tuberculous kidneys expressed hLptn mRNA. HLptn was detected not only in the cells of normal renal glomerulus and renal tubule but also in the cells of remaining renal glomerulus and renal tubule of tuberculous kidneys. The cells expressing surface antigens CD4 and CD8 scattered in granulomas.


CONCLUSIONS
The constructive expression of hLptn is in the cells of renal glomerulus and renal tubule of normal kidney and tuberculous kidney. The accumulation of CD4(+) T cells and CD8(+) T cells in granulomas may not depend on hLptn.
Lu
Xiong-bing
XB
Department of Urology, the Second Affiliated Hospital, Nanchang University, Nanchang 330006, China. luxiongbing123456@yahoo.com.cn
Xie
Hui-li
HL
Zou
Gao-de
GD
Huang
Hong-wei
HW
Xiong
Jian-hua
JH
Shi
Zi-min
ZM
Pan
Zheng-yue
ZY
D015704:CD4 Antigens / Q000032:analysis*; D018791:CD4 Lymphocyte Count; D003602:Cytotoxicity, Immunologic / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000032:analysis; D006099:Granuloma / Q000276:immunology / Q000378:metabolism; D007155:Immunologic Factors / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014398:Tuberculosis, Renal / Q000276:immunology* / Q000378:metabolism
D015704:CD4 Antigens; D018076:DNA, Complementary; D007155:Immunologic Factors