Synthesis of pyrophosphates for in vitro selection of catalytic RNA molecules.
Reported here are synthetic routes to pyrophosphates linking riboflavin with various nucleosides. The focus is on a flavin-uracil dinucleotide having a biotin tag on the uracil, a molecule that has potential value in the selection of RNA enzymes that catalyze the template-directed polymerization of RNA in the 3'-to-5' direction, which is the direction opposite that catalyzed by standard protein polymerases. Two detailed procedures are presented to prepare this new compound, as well as one procedure to prepare the new flavin-2,6-diaminopurine dinucleotide.
Kim
Hyo-joong
HJ
Foundation for Applied Molecular Evolution, Gainesville, Florida, USA.
Kim
Myong Jung
MJ
Karalkar
Nilesh
N
Hutter
Daniel
D
Benner
Steven A
SA
D001710:Biotin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D011756:Diphosphates / Q000138:chemical synthesis* / Q000737:chemistry; D005415:Flavins / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry*; D012256:Riboflavin / Q000737:chemistry
D011756:Diphosphates; D005415:Flavins; D009705:Nucleosides; D016337:RNA, Catalytic; D001710:Biotin; D012256:Riboflavin