Simultaneous determination of levodopa and carbidopa in levodopa-carbidopa tablets by ATR-FTIR spectrometry.
A novel analytical procedure has been developed for quantitative determination of levodopa and carbidopa in aqueous binary solutions acidified by HCl and without any other sample pretreatment. The method is based partially on least squares treatment of data obtained by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectrometry in 1211-1315 cm(-1) and 1488-1550 cm(-1) spectral regions. The simple, rapid, and accurate proposed method was applied to determine levodopa and carbidopa in Levodopa-C tablets. The statistical parameters, such as R2, RSD, SEE, SECV, LOD, and recovery were evaluated. Number of factors, scans, and resolution were optimized. In this method R2 and RSD for levodopa and carbidopa were (0.9965, 1.209) and (0.9537, 0.813), respectively.
Khanmohammadi
Mohammadreza
M
Department of Chemistry, Faculty of Science, Imam Khomeini International University, Qazvin, Iran. mrkhanmohammadi@gmail.com
Mobedi
Elaheh
E
Garmarudi
Amir Bagheri
AB
Mobedi
Hamid
H
Kargosha
Kazem
K
D000978:Antiparkinson Agents / Q000032:analysis*; D002230:Carbidopa / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D004338:Drug Combinations; D007980:Levodopa / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared; D013607:Tablets
D000978:Antiparkinson Agents; D004338:Drug Combinations; D013607:Tablets; D007980:Levodopa; D002230:Carbidopa