In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO(2)-CaO-P(2)O(5) system.
This work describes the evaluation of a glass ceramic (55S41C4P-1300) as a potential substrate for bone tissue engineering. For that purpose, the capacity of mesenchymal stem cells (MSCs), isolated from rabbit bone marrow, to adhere, proliferate and differentiate into osteoblast (OBs) with or without 55S41C4P-1300 was investigated. Two types of culture medium, i.e. growth medium (GM) and osteogenic medium (OM), were evaluated. The bioactive 55S41C4P-1300, containing pseudowollastonite, wollastonite, tricalcium phosphate and crystoballite as crystalline phases, was obtained by heat treatment of a sol-gel glass (55SiO(2), 41CaO, 4P(2)O(5) (mol.%)) at 1300 degrees C. The results showed that the MSCs adhered, spread, proliferated and produced mineralized extracellular matrix on 55S41C4P-1300 regardless of the culture medium used. As the same time, they showed an osteoblastic phenotype, and this phenomenon was accompanied by the gradual diminution of the marker CD90 expression. The 55S41C4P-1300 was able to induce the differentiation of MSCs into OBs in the same way as OM without glass ceramic. This effect increased with the combination of 55S41C4P-1300 with OM. The glass ceramic evaluated in this work is bioactive, cytocompatible and capable of promoting the differentiation of MSCs into OBs. For that reason, it could be regarded as a suitable matrix in tissue engineering for bone tissue regeneration.
Meseguer-Olmo
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Unidad de Investigación Experimental, Laboratorio de Cirugía Ortopédica Experimental, Hospital Universitario V, Arrixaca, Universidad de Murcia, Murcia, Spain. meseguer@arrakis.es
Bernabeu-Esclapez
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A
Ros-Martinez
E
E
Sánchez-Salcedo
S
S
Padilla
S
S
Martín
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AI
Vallet-Regí
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M
Clavel-Sainz
M
M
Lopez-Prats
F
F
Meseguer-Ortiz
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CL
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D003470:Culture Media; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005453:Fluorescence; D005898:Glass / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000378:metabolism; D011817:Rabbits; D013057:Spectrum Analysis; D018800:Thy-1 Antigens / Q000378:metabolism
D003470:Culture Media; D018800:Thy-1 Antigens; C416205:calcium oxide-silicon dioxide-diphosphorus pentoxide glass; D015675:Osteocalcin