Expressed sequence tags from cynomolgus monkey (Macaca fascicularis) liver: a systematic identification of drug-metabolizing enzymes.
The liver, a major organ for drug metabolism, is physiologically similar between monkeys and humans. However, the paucity of identified genes has hampered a deep understanding of drug metabolism in monkeys. To provide such a genetic resource, 28655 expressed sequence tags (ESTs) were generated from a cynomolgus monkey liver full-length enriched cDNA library, which contained 23 unique ESTs homologous to human drug-metabolizing enzymes. Our comparative genomics approach identified nine lineage-specific candidate ESTs, including three drug-metabolizing enzymes, which could be important for understanding the physiological differences between monkeys and humans.
Uno
Yasuhiro
Y
Division of Genetic Diagnosis, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan. unoxx001@pharm.hokudai.ac.jp
Suzuki
Yutaka
Y
Wakaguri
Hiroyuki
H
Sakamoto
Yoshiko
Y
Sano
Hitomi
H
Osada
Naoki
N
Hashimoto
Katsuyuki
K
Sugano
Sumio
S
Inoue
Ituro
I
D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D020224:Expressed Sequence Tags; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013250:Steroid Hydroxylases / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C029009:steroid hormone 7-alpha-hydroxylase