Over-production of a glycoside hydrolase family 50 beta-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product.
The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than that in E. coli. The enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose from agar. Neoagarooligosaccharides produced by the enzyme had a whitening effect and inhibited tyrosinase activity in the murine melanoma cell line, B16F10. Neoagarooligosaccharides were not cytotoxic to B16F10 or normal cells. beta-Agarase could therefore be a good whitening, cosmetic additive.
Lee
Dong-Geun
DG
Department of Pharmaceutical Engineering, College of Medical Life Sciences, Silla University, Busan, 617-736, Korea.
Jang
Min Kyung
MK
Lee
Ok-Hee
OH
Kim
Nam Young
NY
Ju
Seong-A
SA
Lee
Sang-Hyeon
SH
D000362:Agar / Q000378:metabolism; D034241:Alteromonadaceae / Q000201:enzymology*; D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004187:Disaccharides / Q000378:metabolism; D005697:Galactosides / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism*; D008543:Melanins / Q000378:metabolism; D008544:Melanocytes / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D009844:Oligosaccharides / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
D004187:Disaccharides; D005697:Galactosides; D008543:Melanins; D009844:Oligosaccharides; D011994:Recombinant Proteins; C081798:neoagarotetraose; C068544:neoagarobiose; D000362:Agar; D014442:Monophenol Monooxygenase; D006026:Glycoside Hydrolases; C019624:agarase