title: [Effects of bone marrow-derived mesenchymal stem cells transplantation on recovery of neurological functions and expression of synaptophysin in focal cerebral infarction in rats].
abstract: OBJECTIVE
  To investigate the effects of bone marrow-derived mesenchymal stem cells (BMSC) transplantation on the recovery of neurological functions and the expression of synaptophysin in focal cerebral infarction in rats.
  
  
  METHODS
  72 male adult Sprague-Dawley rats were randomly divided into 4 groups (18 in each group): shamoperated group, middle cerebral artery occlusion (MCAO) roup,vehicle group and MCAO+BMSC-treated group. A permanent focal cerebral ischemia model was established using modified Longa's method. BMSC was labeled by DAPI before the transplantation. One day after right middle cerebral artery occlusion(MCAO), 1 x 10(6) cells were injected into the lateral ventricle of rats in BMSCs-treated group and the same dose of PBS was given to the rats in vehicle group. Before sacrificed and at 4 d, 7 d and 14 d after MCAO, the neurological functions were tested by balance beam, rota-rod and screen prehensile and the synaptophysin was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical method.
  
  
  RESULTS
  DAPI stained positive cells were observed around the cerebral infarcted area in the BMSC-treated group. Compared with the MCAO group and the vehicle group,the neurological functions in BMSC-treated group were better on 7 d and 14 d after MCAO (P<0.05 or P<0.01), and the synaptophysin around the cerebral infarcted area was significantly upregulated on 4 d, 7 d and 14 d after MCAO (all P<0.01).
  
  
  CONCLUSIONS
  BMSC transplantation can improve the neurological functions by upregulating the expression of synaptophysin after MCAO in rats.
authors.affiliation: Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou 350001, China. wjs5227705@sina.com.cn
authors.forename: Jin-sen
authors.initials: JS
authors.lastname: Weng
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.forename: Hou-wei
authors.initials: HW
authors.lastname: DU
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Yi-xian
authors.initials: YX
authors.lastname: Zhang
authors.forename: Jie-hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Hua-pin
authors.initials: HP
authors.lastname: Huang
mesh_terms: D000328:Adult; D000818:Animals; D002544:Cerebral Infarction / Q000235:genetics / Q000378:metabolism*; D005652:Functional Residual Capacity; D015870:Gene Expression; D006801:Humans; D008264:Macrophages; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016708:Synaptophysin / Q000235:genetics / Q000378:metabolism*
chemical_list: D016708:Synaptophysin