[Detection of angiotensin II in the maternal-fetal interface in pregnancy-induced hypertension].
OBJECTIVE
To detect angiotensin II (ANG II) in the maternal blood, umbilical cord blood, and at the maternal-fetal interface in women with pregnancy-induced hypertension (PIH) and explore the etiology of PIH and pathophysiologic mechanism of intrauterine growth restriction (IUGR).


METHODS
Enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of ANG II in the maternal blood, cord blood, and maternal and fetal placental tissues in 30 women with PIH and 30 with normal pregnancy, and the results were analyzed with independent-sample t test and Pearson correlation analysis.


RESULTS
ANG II level in the maternal placental tissue homogenate showed no significant difference between women with PIH and normal pregnant women (8.51+/- 4.01 vs 7.76+/-3.47 pg/ml, P>0.05), but women with PIH had significantly higher ANG II in the fetal placental tissue (11.82+/-3.92 vs 9.64+/-2.63 pg/ml, P<0.05). ANG II level was significantly higher in the maternal blood of women with PIH than in normal pregnant women (46.44+/-8.48 vs 32.43+/-5.87 pg/ml, P<0.001), but similar in the cord blood (68.83+/-8.68 vs 72.47+/-8.51 pg/ml; P>0.05). A positive correlation was indicated between the cord blood and maternal peripheral blood ANG II levels in women with PIH (r=0.7379, P<0.05).


CONCLUSIONS
ANG II in the fetal placental tissue is elevated, and the cord blood and maternal peripheral blood ANG II levels are positively correlated in women with PIH.
Tao
Shao-hua
SH
Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. tsh1987@sina.com
Feng
Zhi-chun
ZC
DU
Jiang
J
D000804:Angiotensin II / Q000097:blood* / Q000302:isolation & purification; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D005317:Fetal Growth Retardation / Q000097:blood; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood*; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy
D000804:Angiotensin II