Up-regulation of hyaluronan synthase genes in cultured human epidermal keratinocytes by UVB irradiation.
Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and both were up-regulated after a single irradiation with moderate UVB but hyaluronidases was unchanged. Increased accumulation of hyaluronan in the culture medium mirrored the UVB-induced increase in the mRNA levels of HAS3 and HAS2. Unexpectedly, hyaluronan derived from UVB-irradiated and non-irradiated cells had identical size distribution. Increased expression of KGF and IL-1beta was detected just prior to the increase of HAS3 and HAS2 mRNAs after UVB irradiation. Antibody-neutralization study revealed that KGF and/or IL-1beta were at least involved in the up-regulation of HAS3 and HAS2 expressions. UVB-irradiated cells may enhance hyaluronan production to maintain homeostasis through up-regulation of HAS3 and HAS2 genes via cytokine response mechanism.
Kakizaki
Ikuko
I
Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan. kaki@cc.hirosaki-u.ac.jp
Itano
Naoki
N
Kimata
Koji
K
Hanada
Katsumi
K
Kon
Atsushi
A
Yamaguchi
Masanori
M
Takahashi
Teruno
T
Takagaki
Keiichi
K
D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology / Q000528:radiation effects; D014453:Glucuronosyltransferase / Q000096:biosynthesis* / Q000235:genetics* / Q000528:radiation effects; D006801:Humans; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000096:biosynthesis / Q000528:radiation effects; D015603:Keratinocytes / Q000166:cytology / Q000201:enzymology* / Q000528:radiation effects*; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000528:radiation effects*
D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases