title: Genetic analysis of spontaneous lactose-utilizing mutants from vibrio vulnificus.
abstract: Wild-type V. vulnificus cannot grow using lactose as the sole carbon source or take up the sugar. However, prolonged culture of this species in media containing lactose as the sole carbon source leads to the generation of a spontaneous lactose-utilizing (LU) mutant. This mutant showed strong beta- galactosidase activity, whereas the wild-type strain showed a barely detectable level of the activity. A mutant with a lesion in a gene homologous to the lacZ of E. coli in the bacterium no longer showed beta-galactosidase activity or generated spontaneous LU mutants, suggesting that the lacZ homolog is responsible for the catabolism of lactose, but the expression of the gene and genes for transport of lactose is tightly regulated. Genetic analysis of spontaneous LU mutants showed that all the mutations occur in a lacI homolog, which is located downstream to the lacZ and putative ABC-type lac permease genes. Consistent with this, a genomic library clone containing the lacI gene, when present in trans, made the spontaneous LU mutants no longer able to utilize lactose as the sole carbon source. Taken together with the observation that excessive amounts of exogenously supplemented possible catabolic products of lactose have negative effects on the growth and survivability of V. vulnificus, we suggest that V. vulnificus has evolved to carry a repressor that tightly regulates the expression of lacZ to keep the intracellular toxic catabolic intermediates at a sublethal level.
authors.affiliation: Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Baek
authors.forename: Ko-Eun
authors.initials: KE
authors.lastname: Lee
authors.forename: Dae-Kyun
authors.initials: DK
authors.lastname: Park
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Choi
authors.forename: Kun-Soo
authors.initials: KS
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007785:Lactose / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014169:Transformation, Bacterial; D041261:Vibrio vulnificus / Q000235:genetics* / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012097:Repressor Proteins; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose; D005690:Galactose