title: [Inhibition of salicylate on voltage-gated sodium channels in rat inferior colliculus neurons].
abstract: OBJECTIVE
  To investigate the mechanism of the tinnitus inducer, sodium salicylate, on voltage-gated sodium channels.
  
  
  METHODS
  The effects of salicylate on voltage-gated sodium channels in freshly dissociated inferior colliculus neurons of rats were studied, using the whole-cell voltage clamp method.
  
  
  RESULTS
  Salicylate blocked sodium current (INa) in concentration-dependent manner (0.1-10 mmol/L). The IC50 value for the blocking action of salicylate was 1.43 mmol/L. Salicylate did not affect the conductance-voltage curve and the steady-state activation curve of INa. The steady-state INa inactivation curve of INa was shifted by about 9 mV in the hyperpolarizing direction. In addition, salicylate delayed the sodium channel recovery from INa inactivation by increasing the slow time constant.
  
  
  CONCLUSIONS
  Our results suggest that salicylate causes a concentration-dependent blockade of INa and shifts the INa inactivation curve to more hyperpolarized potentials, which could be related to the mechanism of salicylate-induced tinnitus.
authors.affiliation: Department of Otorhinolaryngology, Peking University Third Hospital, Beijing, China.
authors.forename: Yanxing
authors.initials: Y
authors.lastname: Liu
authors.forename: Xuepei
authors.initials: X
authors.lastname: Li
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.forename: Yongli
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Xiang
authors.initials: X
authors.lastname: Qi
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D007245:Inferior Colliculi / Q000166:cytology; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D015222:Sodium Channels / Q000187:drug effects* / Q000378:metabolism; D012980:Sodium Salicylate / Q000494:pharmacology*
chemical_list: D015222:Sodium Channels; D012980:Sodium Salicylate