[Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring].
OBJECTIVE
To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood.


METHODS
This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multi-ple-reaction monitoring (MRM). After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode.


RESULTS
Identification was based on the compound's retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1 ng/mL to 20 ng/mL in blood.


CONCLUSIONS
The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.
Liu
Wei
W
Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Shen
Min
M
Shen
Bao-hua
BH
Xiang
Ping
P
Wu
He-jian
HJ
D000157:Aconitine / Q000097:blood; D000470:Alkaloids / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D003078:Colchicine / Q000097:blood; D005554:Forensic Medicine; D006801:Humans; D012680:Sensitivity and Specificity; D013331:Strychnine / Q000097:blood; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000470:Alkaloids; D013331:Strychnine; D003078:Colchicine; D000157:Aconitine