title: Shh influences cell number and the distribution of neuronal subtypes in dorsal root ganglia.
abstract: The molecular mechanisms responsible for specifying the dorsal-ventral pattern of neuronal identities in dorsal root ganglia (DRG) are unclear. Here we demonstrate that Sonic hedgehog (Shh) contributes to patterning early DRG cells. In vitro, Shh increases both proliferation and programmed cell death (PCD). Increasing Shh in vivo enhances PCD in dorsal DRG, while inducing greater proliferation ventrally. In such animals, markers characteristic of ventral sensory neurons are expanded to more dorsal positions. Conversely, reducing Shh function results in decreased proliferation of progenitors in the ventral region and decreased expression of the ventral marker trkC. Later arising trkA(+) afferents make significant pathfinding errors in animals with reduced Shh function, suggesting that accurate navigation of later arising growth cones requires either Shh itself or early arising, Shh-dependent afferents. These results indicate that Shh can regulate both cell number and the distribution of cell types in DRG, thereby playing an important role in the specification, patterning and pathfinding of sensory neurons.
authors.affiliation: Interdepartmental Program in Neuroscience, University of Utah, School of Medicine, 20 North 1900 East, Salt Lake City, UT 84132-3401, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Guan
authors.forename: Guoying
authors.initials: G
authors.lastname: Wang
authors.forename: Sheryl A
authors.initials: SA
authors.lastname: Scott
authors.forename: Maureen L
authors.initials: ML
authors.lastname: Condic
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D018929:Cell Culture Techniques; D002455:Cell Division; D002642:Chick Embryo / Q000166:cytology* / Q000502:physiology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology*; D053823:Hedgehog Proteins / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D020287:In Situ Nick-End Labeling; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology
chemical_list: D053823:Hedgehog Proteins; D001973:Bromodeoxyuridine