[Parasitological and epidemiological aspects of Trypanosoma cruzi seropositive blood donors].
BACKGROUND
After the interruption of the transmission of Chagas disease via vector insects in Chile, there is little available epidemiological information about this parasitosis in blood banks.


OBJECTIVE
To update the rates of T cruzi positive blood donors. To measure parasitological and epidemiological parameters in blood donors with anti T cruzi antibodies.


METHODS
An ELISA-T cruzi test was carried out in 30,309 blood donors between 2000 and 2004. In 75 blood donors with an ELISA-T cruzi positive test and 79 donors with negative ELISA (controls), a survey about personal or parental history of biting by a kissing bug (Triatomine), was performed. A blood sample was also obtained to perform Polymerase Chain Reaction (PCR) for T cruzi and a xenodiagnostic test.


RESULTS
Annual frequency of positive ELISA for T cruzi serum antibodies in blood donors varied from 0.31% to 0.45%. Twenty eight percent of subjects with positive and 6% of subjects with negative specific antibodies answered the survey about biting. PCR and xenodiagnostic test were positive in 52 (69%) and 16 (21%) of positive ELISA-T cruzi test blood donors, respectively. Xenodiagnostic was also positive in 5 individuals who had a negative PCR.


CONCLUSIONS
Seroprevalence of T cruzi antibodies decreased from 3% in 1968 to 0.3% in 2004.
Galaz
Pablo
P
Unidad Docente de Parasitología, Facultad de Medicina, Universidad de ChileSantiago, Chile.
García
Stephanie
S
Mercado
Rubén
R
Orrego
Elsa
E
Pagliero
Blanca
B
Contreras
María Del Carmen
Mdel C
Salinas
Patricia
P
Arancibia
Carlos
C
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001782:Blood Donors; D014355:Chagas Disease / Q000175:diagnosis / Q000453:epidemiology*; D002677:Chile / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016036:Seroepidemiologic Studies; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology*; D020525:Xenodiagnosis
D000913:Antibodies, Protozoan