title: Constitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in Saccharomyces cerevisiae.
abstract: The endoxylanase (GenBank Access No. U51675) of Bacillus spp. and endoglucanase (GenBank Access No. AY466436) of Trichoderma spp. were separately inserted downstream of the yeast constitutive ADH1 promoter, resulting in three different plasmids (pAGX1, pAGX2, and pAGX3) according to the transcription direction of two genes. When the yeast transformants, S. cerevisiae SEY2102 harboring each expression plasmid, were grown on YPD medium, the total activities of the enzymes were approximately 3.01 unit/ml, 3.24 unit/ml, and 7.56 unit/ml for endoxylanase and 0.60 unit/ml, 0.54 unit/ml, and 0.39 unit/ ml for endoglucanase, in the following order: the pAGX1, pAGX2, and pAGX3. More than 70% of the endoxylanase and endoglucanase activities was found in the extracellular media.
authors.affiliation: Department of Biomaterial Control (BK21 Program), Dong-Eui University, Busan 614-714, Korea.
authors.forename: Jae Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Myung-Ye
authors.initials: MY
authors.lastname: Lim
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Seo
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Soo-Wan
authors.initials: SW
authors.lastname: Nam
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014170:Transformation, Genetic; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics; D014990:Xylans / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D014990:Xylans; D002480:Cellulase; C019826:carboxymethylcellulase; D043364:Endo-1,4-beta Xylanases