title: The 14-3-3 protein affects the conformation of the regulatory domain of human tyrosine hydroxylase.
abstract: Tyrosine hydroxylase (TH) catalyzes the first step in the biosynthesis of catecholamines. Regulation of TH enzyme activity is controlled through the posttranslational modification of its regulatory domain. The regulatory domain of TH can be phosphorylated at four serines (8, 19, 31, and 40) by a variety of protein kinases. Phosphorylation of Ser19 does not by itself increase TH activity but induces its binding to the 14-3-3 protein. That leads to the enhancement of TH activity with a still not fully understood mechanism. The main goal of this work was to investigate whether the 14-3-3 protein binding affects the conformation of the regulatory domain of human TH isoform 1 (TH1R). Site-directed mutagenesis was used to generate five single-tryptophan mutants of TH1R with the Trp residue located at five different positions within the domain (positions 14, 34, 73, 103, and 131). Time-resolved tryptophan fluorescence measurements revealed that phosphorylation of Ser19 and Ser40 does not itself induce any significant structural changes in regions surrounding inserted tryptophans. On the other hand, the interaction between the 14-3-3 protein and phosphorylated TH1R decreases the solvent exposure of tryptophan residues at positions 14 and 34 and induces distinct structural change in the vicinity of Trp73. The 14-3-3 protein binding also reduces the sensitivity of phosphorylated TH1R to proteolysis by protecting its N-terminal part (first 33 residues). Circular dichroism measurements showed that TH1R is an unstructured protein with a low content of secondary structure and that neither phosphorylation nor the 14-3-3 protein binding changes its secondary structure.
authors.affiliation: Institute of Physiology, Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Obsilova
authors.forename: Eliska
authors.initials: E
authors.lastname: Nedbalkova
authors.forename: Jan
authors.initials: J
authors.lastname: Silhan
authors.forename: Evzen
authors.initials: E
authors.lastname: Boura
authors.forename: Petr
authors.initials: P
authors.lastname: Herman
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Vecer
authors.forename: Miroslav
authors.initials: M
authors.lastname: Sulc
authors.forename: Jan
authors.initials: J
authors.lastname: Teisinger
authors.forename: Fred
authors.initials: F
authors.lastname: Dyda
authors.forename: Tomas
authors.initials: T
authors.lastname: Obsil
mesh_terms: D048948:14-3-3 Proteins / Q000502:physiology*; D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D014446:Tyrosine 3-Monooxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; D014446:Tyrosine 3-Monooxygenase