title: Sphingosine-1-phosphate induces the association of membrane-type 1 matrix metalloproteinase with p130Cas in endothelial cells.
abstract: Membrane-type 1 matrix metalloproteinase (MT1-MMP) plays an important role in sphingosine-1-phosphate(S1P)-dependent migration of endothelial cells but the underlying mechanisms remain largely unknown. Herein, we show that S1P promotes the relocalization of MT1-MMP to peripheral actin-rich membrane ruffles that is coincident with its association with the adaptor protein p130Cas at the leading edge of migrating cells. Immunoprecipitation and confocal microscopy analyses suggest that this interaction required the tyrosine phosphorylation of p130Cas and also involves S1P-dependent phosphorylation of MT1-MMP within its cytoplasmic sequence. The interaction of MT1-MMP with p130Cas at the cell periphery suggests the existence of a close interplay between pericellular proteolysis and signaling pathways involved in EC migration.
authors.affiliation: Laboratoire de Médecine Moléculaire, Hôpital Ste-Justine-Université du Québec à Montréal, Centre de Cancérologie Charles-Bruneau, 3175 Chemin Côte-Ste-Catherine, Montréal, Québec, Canada H3T 1C5.
authors.forename: Denis
authors.initials: D
authors.lastname: Gingras
authors.forename: Marisol
authors.initials: M
authors.lastname: Michaud
authors.forename: Geneviève
authors.initials: G
authors.lastname: Di Tomasso
authors.forename: Eric
authors.initials: E
authors.lastname: Béliveau
authors.forename: Carine
authors.initials: C
authors.lastname: Nyalendo
authors.forename: Richard
authors.initials: R
authors.lastname: Béliveau
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D050719:Crk-Associated Substrate Protein / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D011499:Protein Processing, Post-Translational / Q000502:physiology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014018:Tissue Distribution
chemical_list: D050719:Crk-Associated Substrate Protein; D008246:Lysophospholipids; C060506:sphingosine 1-phosphate; D011505:Protein-Tyrosine Kinases; D053511:Matrix Metalloproteinase 14; D013110:Sphingosine