Applicability of phylogenetic methods for characterizing the public health significance of verocytotoxin-producing Escherichia coli strains.
Two phylogenetic methods (multilocus sequence typing [MLST] and a multiplex PCR) were investigated to determine whether phylogenetic classification of verocytotoxin-producing Escherichia coli serotypes correlates with their classification into groups (seropathotypes A to E) based on their relative incidence in human disease and on their association with outbreaks and serious complications. MLST was able to separate 96% of seropathotype D and E serotypes from those that cause serious disease (seropathotypes A to C), whereas the multiplex PCR lacked this level of seropathotype discrimination.
Ziebell
Kim
K
Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada.
Konczy
Paulina
P
Yong
Irene
I
Frost
Shelley
S
Mascarenhas
Mariola
M
Kropinski
Andrew M
AM
Whittam
Thomas S
TS
Read
Susan C
SC
Karmali
Mohamed A
MA
D001483:Base Sequence; D016000:Cluster Analysis; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D005798:Genes, Bacterial / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011634:Public Health / Q000379:methods; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D022622:Shiga Toxin 1 / Q000378:metabolism*; D013045:Species Specificity; D014774:Virulence
D022622:Shiga Toxin 1