Mycothiol disulfide reductase: solid phase synthesis and evaluation of alternative substrate analogues.
A solid phase synthesis of des-myo-inositol mycothiol disulfide and its alpha-configured methyl- and benzyl-glycoside derivatives has been developed. Kinetic characterisation of these compounds has demonstrated their viability as alternative substrates for use in mycothiol disulfide reductase enzyme assays.
Stewart
Matthew J G
MJ
School of Chemistry and Chemical Engineering, David Keir Building, Stranmillis Road, Queen's University Belfast, Belfast, United Kingdom BT9 5AG.
Jothivasan
Vishnu Karthik
VK
Rowan
Andrew S
AS
Wagg
Jennifer
J
Hamilton
Chris J
CJ
D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques; D003545:Cysteine / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004220:Disulfides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D006027:Glycosides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D007294:Inositol / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D008968:Molecular Conformation; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D004220:Disulfides; D004791:Enzyme Inhibitors; D006020:Glycopeptides; D006027:Glycosides; C089265:mycothiol; D007294:Inositol; D009247:NADH, NADPH Oxidoreductases; C089267:disulfide reductase, mycothiol; D003545:Cysteine