Different patterns of integral membrane protein localization during cell division in Bacillus subtilis.
Cell division in rod-shaped bacteria nearly always occurs exactly at mid-cell and is dependent on the formation of the cytokinetic FtsZ ring and its associated division proteins. Many thousands of copies of division, or septum-specific proteins assemble at this site and may lead to the exclusion of other integral membrane proteins that are normally able to diffuse freely throughout the cytoplasmic membrane. In this study we have investigated the localization of a series of integral membrane proteins in Bacillus subtilis and we show that the recruitment of division and septum-specific proteins does not necessarily preclude the diffusion of other integral membrane proteins. However, some proteins, namely ATP synthase and succinate dehydrogenase, are reduced/absent from the mid-cell region at the onset of cell division, which may reflect an association with lipid domains rich in phosphatidylglycerol that are thought to be present at diminished levels at sites of cell division.
Meredith
D H
DH
School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.
Plank
M
M
School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.
Lewis
P J
PJ
School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.
D001412:Bacillus subtilis / Q000737:chemistry* / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D002455:Cell Division / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008565:Membrane Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence
D001426:Bacterial Proteins; C485182:Cyan Fluorescent Protein; D008164:Luminescent Proteins; D008565:Membrane Proteins; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins