Electrochemical detection of kinase-catalyzed phosphorylation using ferrocene-conjugated ATP.
Adenosine-5'-[gamma-ferrocene] triphosphate is exploited as a co-substrate for the phosphorylation of the surface-immobilized peptide C-SIYRRGSRRWRKL by protein kinase C, in which the gamma-ferrocene phosphate is transferred to the peptide and then detected by cyclic voltammetry.
Song
Haifeng
H
Department of Chemistry, University of Western Ontario, London, Ontario, Canada.
Kerman
Kagan
K
Kraatz
Heinz-Bernhard
HB
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D013058:Mass Spectrometry; D000075163:Metallocenes; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*
D005296:Ferrous Compounds; D000075163:Metallocenes; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; C004998:ferrocene