Ca(2+)- and glycoconjugates-dependent prey capture in the heliozoon Actinophrys sol.
Exocytosis of extrusomes, secretory granules found in protozoa, is involved in prey capture by the heliozoon Actinophrys sol. Here, we show that extracellular Ca(2+) is necessary for exocytosis and prey capture in A. sol. We found that A. sol could not capture prey cells in a Ca(2+)-free solution. L-type Ca(2+) channel blockers and a calmodulin antagonist also inhibited the capture of prey. These results suggest that Ca(2+) influx via L-type Ca(2+) channels plays a crucial role in exocytosis in A. sol. Concanavalin A (Con A) also inhibited prey capture, and the inhibition was relieved by the addition of its hapten sugar, alpha-mannoside, suggesting that Con A-binding glycoconjugates are implicated in exocytosis of extrusomes and the adhesion of prey cells.
Kakuta
Soichiro
S
Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
Suzaki
Toshinobu
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type; D003208:Concanavalin A / Q000378:metabolism; D056890:Eukaryota / Q000502:physiology*; D005089:Exocytosis; D006001:Glycoconjugates / Q000378:metabolism*; D011235:Predatory Behavior / Q000502:physiology*
D020746:Calcium Channels, L-Type; D006001:Glycoconjugates; D003208:Concanavalin A; D002118:Calcium