title: Quantification of blood group A and B antibodies by flow cytometry using beads carrying A or B trisaccharides.
abstract: In the clinical management of patients receiving blood group ABO-incompatible organ allografts, it is of importance to determine the levels of blood group A and B antibodies before and after transplant. Currently used methods, which are mostly based on hemagglutination, are inexact and are associated with large intercenter variations. Here, we describe preliminary data from our efforts to establish a flow cytometry-based assay for the semiquantification of blood group A and B antibodies using beads carrying synthetic A or B trisaccharides. In agreement with previous investigations, blood group O individuals had greater levels of anti-A immunoglobulin G (IgG) than B individuals, whereas the levels of anti-A immunoglobulin M (IgM) were similar in sera from blood group O and B individuals.
authors.affiliation: AbSorber AB, Stockholm, Sweden.
authors.forename: Maria
authors.initials: M
authors.lastname: Sundbäck
authors.forename: Per
authors.initials: P
authors.lastname: Grufman
authors.forename: Joachim
authors.initials: J
authors.lastname: Teller
authors.forename: Fritz
authors.initials: F
authors.lastname: Westphal
authors.forename: Elena
authors.initials: E
authors.lastname: Korchagina
authors.forename: Nicolai
authors.initials: N
authors.lastname: Bovin
authors.forename: Jan
authors.initials: J
authors.lastname: Holgersson
mesh_terms: D000017:ABO Blood-Group System; D000327:Adsorption; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M / Q000737:chemistry; D007164:Immunosorbents / Q000737:chemistry; D033581:Stem Cell Transplantation; D014180:Transplantation / Q000379:methods*; D014312:Trisaccharides / Q000737:chemistry*
chemical_list: D000017:ABO Blood-Group System; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D007164:Immunosorbents; D014312:Trisaccharides