Expression of monocyte markers in HIV-1 infected individuals with or without HIV associated dementia and normal controls in Bangkok Thailand.
HIV Associated Dementia (HAD) is a complication of HIV infection in developed countries and is still poorly defined in resource-limited settings. In this study we investigated the expression of the monocyte phenotype CD14CD16HLADR and the inflammatory profiles in monocytes supernatants by surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry in a cohort of HAD and non-HAD Thai volunteers prior to the initiation of ARV. The CD14CD16HLADR phenotype was significantly increased in monocytes from HAD and non-HAD versus negative controls, but there was no difference in phenotype and in the secretion protein profiles between the two seropositive groups. In addition, monocytes supernatants from HAD and non-HAD did not induced apoptosis or cell death in brain aggregate culture. In conclusion it appears that HAD in Thai individuals has a different immunological profile then in North America cohorts.
Ratto-Kim
Silvia
S
Hawaii AIDS Clinical Research Program, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, United States. silvia.kim@afrims.org
Chuenchitra
Thippawan
T
Pulliam
Lynn
L
Paris
Robert
R
Sukwit
Suchitra
S
Gongwon
Siriphan
S
Sithinamsuwan
Pasiri
P
Nidhinandana
Samart
S
Thitivichianlert
Sataporn
S
Shiramizu
Bruce T
BT
de Souza
Mark S
MS
Chitpatima
Suwicha T
ST
Sun
Bing
B
Rempel
Hans
H
Nitayaphan
Sorachai
S
Williams
Kenneth
K
Kim
Jerome H
JH
Shikuma
Cecilia M
CM
Valcour
Victor G
VG
D015526:AIDS Dementia Complex / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D015704:CD4 Antigens / Q000378:metabolism*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D015658:HIV Infections / Q000378:metabolism / Q000473:pathology*; D015497:HIV-1; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018709:Statistics, Nonparametric; D013785:Thailand / Q000453:epidemiology; D019562:Viral Load
D015704:CD4 Antigens