Real-time observation of single-molecule fluorescence in microdroplet streams.
We report real-time observation of fluorescence bursts from individual Rhodamine 6G molecules in streams of microdroplets (peak signal-to-noise ratios, approximately 30) whose trajectories are constrained with a linear electric quadrupole. This approach offers a reasonable dynamic range in droplet size (3- 12-microm diameter) with <1% shot-to-shot size fluctuations and sensitivity comparable with that of droplet levitation techniques with at least 10(3) higher analysis rates. Applications to the study of single-molecule microcavity effects and stimulated emission are discussed.
Barnes
M D
MD
Lermer
N
N
Kung
C Y
CY
Whitten
W B
WB
Ramsey
J M
JM
Hill
S C
SC