Pichia koratensis sp. nov., a new ascomycetous yeast related to Pichia acaciae isolated from insect frass in Thailand.
Two yeast strains (ST-235 and ST-237) isolated from insect frass collected in northeastern Thailand, were identified as Pichia acaciae and a hitherto undescribed species based on the sequence analysis of the D1/D2 domain of 26S rDNA, ITS regions, and DNA-DNA reassociation experiments. The latter strain, ST-237 (=BCC 11769=NBRC 103638=JCM 12576), was described as Pichia koratensis sp. nov. Pichia koratensis is closely related to Pichia acaciae in the D1/D2 domain sequence of 26S rDNA and phenotypical characteristics but differs by 5 nucleotides (0.9%) from the type strain of P. acaciae and is clearly distinguished by the assimilation and fermentation of sucrose.
Nakase
Takashi
T
Central Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand. nakase-takashi@nite.go.jp
Jindamorakot
Sasitorn
S
Mikata
Kozaburo
K
Ninomiya
Shinya
S
Kawasaki
Hiroko
H
Limtong
Savitree
S
Potacharoen
Wanchern
W
Tanticharoen
Morakot
M
D000818:Animals; D007313:Insecta / Q000382:microbiology*; D010802:Phylogeny; D010843:Pichia / Q000145:classification* / Q000302:isolation & purification; D013045:Species Specificity; D013785:Thailand