Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells.
Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP) were specifically expressed in the Sca-1(+) bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.
Choi
Seung-Cheol
SC
Department of Cardiology, College of Medicine, Korea University, Seoul, 136-705, Republic of Korea.
Shim
Wan-Joo
WJ
Lim
Do-Sun
DS
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018189:Immunomagnetic Separation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D042787:Receptor, TIE-2 / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
D018994:Myosin Light Chains; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D042787:Receptor, TIE-2; D040301:Vascular Endothelial Growth Factor Receptor-2