Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR.
A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.
Landry
Marie L
ML
Department of Laboratory Medicine, Yale New Haven Hospital, and Yale University School of Medicine, New Haven, CT 06520-8035, USA. marie.landry@yale.edu
Cohen
Sandra
S
Ferguson
David
D
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D007223:Infant; D029121:Metapneumovirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase
D000911:Antibodies, Monoclonal; D019914:Taq Polymerase