[Effects of Feixianping on collagen type I and III in bleomycin induced pulmonary fibrosis rats].
OBJECTIVE
To study the effect of Feixianping (FXP) on collagen type I and II in rats with pulmonary fibrosis (PF).


METHODS
Sixty healthy male SD rats were randomly divided into 5 groups, the normal group (A), the model group (B), the positive control group (C) and the two FXP groups (D and E) treated respectively with high and low dose of FXP. Except those in Group A (they were not modeled and administered with normal saline), all rats were established into PF model by intra-tracheal instillation of bleomycin and administered with respective medicines starting from the 1st day after modeling. Rats were sacrificed in batches at 3 time points, the 7th, 14th, and 28th day for observing the pathological changes of lung under light microscope with HE staining and to identify collagen type I and III in lung tissue by immunohistochemical stain and image quantitative analysis.


RESULTS
Light-dyeing proliferative collagen fiber was presented in the slightly thickened alveolar wall in lung of modeled rats from the 14th day on, and the pathological changes became more distinct on the 28th day. The highest amount of collagen appeared in the group B, correspondingly, that in all the other groups was much lower (P < 0.05). Reduction of collagen type I and III revealed in both FXP treated groups, but better effect was shown in the high dose FXP group. The effect of FXP was superior to that of positive control on the 14 th day (P <0.05).


CONCLUSIONS
FXP can effectively reduce the abnormal proliferation of collagen in experimental rats with PF.
Zhang
Yan-ping
YP
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing. zhangyp1953@sohu.com
Fan
Mao-rong
MR
Wang
Shu-chen
SC
D000818:Animals; D001761:Bleomycin; D024042:Collagen Type I / Q000032:analysis*; D024061:Collagen Type III / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D007150:Immunohistochemistry; D008297:Male; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D024042:Collagen Type I; D024061:Collagen Type III; D004365:Drugs, Chinese Herbal; C533490:feixianping; D001761:Bleomycin