title: [Determination of tetrandrine and fangchinoline in Radix Stephaniae tetrandrae and its preparation by nonaqueous capillary chromatography].
abstract: OBJECTIVE
  To establish a new method for the determination of fangchinoline and tetrandrine in Stephania tetrandra and Fengtongan capsule by noanqueous capillary electrophoresis.
  
  
  METHODS
  Separation was carried out in an uncoated fused capillary (50 cm x 75 microm i.d.) with a running buffer containing 50 mmol x L(-1) ammonium acetate, 1.0% acetic acid and 20% acetonitrile in methanol. A separation voltage of 20 kV and a UV detector wavelength at 214 nm were adopted. Sample was introduced from the anode.
  
  
  RESULTS
  The calibration ranges were 1.00, 500 mg x L(-1) for both analytes. Under the optimum conditions, the relative standard deviation (RSD, n = 6) for the migration time of each analyte were 0.09%, 1.9% (intra-day) and 0.63%, 1.9% (inter-day); The RSD for the peak area of each analyte were 0.45%, 5.9% (intra-day) and 2.3%, 5.6% (inter-day), respectively. The contents of the analytes were determined easily with average recoveries 102% for fangchinoline and 105% for tetrandrine in S. tetrandra and 94.6% for fangchinoline and 98.7% for tetrandrine in Fengtongan capsules, respectively.
  
  
  CONCLUSIONS
  The proposed method is simple, rapid, accurate and higher repeatable, and can be used to control of the quality of S. tetrandra and Fengtongan capsules.
authors.affiliation: College of Pharmacy, Taishan Medicine College, Taian 271016, China. lqq29@163.com
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Li
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Chen
authors.forename: Yong-Xiu
authors.initials: YX
authors.lastname: Qi
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Bao-Xiu
authors.initials: BX
authors.lastname: Jia
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Liu
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Cao
mesh_terms: D044182:Benzylisoquinolines / Q000032:analysis*; D002138:Calibration; D053801:Capillary Electrochromatography / Q000379:methods*; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D031609:Stephania tetrandra / Q000737:chemistry*
chemical_list: D044182:Benzylisoquinolines; D002214:Capsules; D004365:Drugs, Chinese Herbal; C009438:tetrandrine; C060802:fangchinoline