Transient focal cerebral ischemia induces a dramatic activation of small ubiquitin-like modifier conjugation.
This study was designed to investigate whether small ubiquitin-like modifier (SUMO) conjugation is activated after focal cerebral ischemia. Transient ischemia induced a dramatic increase in SUMO2/3 protein conjugates. The most pronounced changes were found in the parietal cortex. SUMO2/3 conjugation was particularly high in neurons located at the border of the middle cerebral artery territory where sumoylated proteins translocated to the nucleus. Considering the marked effect of SUMO conjugation on the function of target proteins, it is very likely that the postischemic activation of sumoylation has a significant effect on the fate of neurons exposed to transient ischemia.
Yang
Wei
W
Department of Anesthesiology, Multidisciplinary Neuroprotection Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA.
Sheng
Huaxin
H
Warner
David S
DS
Paschen
Wulf
W
D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism* / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010296:Parietal Lobe / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D025842:SUMO-1 Protein / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*
D025842:SUMO-1 Protein; C529669:SUMO2 protein, rat; C529670:SUMO3 protein, rat; D025841:Small Ubiquitin-Related Modifier Proteins