title: Infectivity of the cloned components of a begomovirus: DNA beta complex causing chilli leaf curl disease in India.
abstract: The full-length genome of a begomovirus and its cognate DNA-beta satellite component associated with chilli leaf curl disease (ChLCD), originating from Varanasi, India, were cloned. Sequence analysis revealed that the viral genome (EF190217) is 2,750 bp and the DNA-beta satellite (EF190215) is 1,361 bp in length. Agroinoculation with partial tandem repeats of the viral genome along with the satellite induced symptoms typical of ChLCD in chilli and Nicotiana benthamiana. However, symptom expression was delayed and milder when the viral genome was agroinoculated alone in these hosts. Sequence comparisons revealed that the genome had the highest sequence identity (95%) with that of chilli leaf curl virus-PK[PK:Mul:98]. The DNA-beta satellite shared maximum sequence identity (88%) with a DNA-beta satellite associated with tomato leaf curl disease from Rajasthan (ToLCBDB-[IN:Raj:03]). These results demonstrate that ChLCD is caused by a complex consisting of the monopartite chilli leaf curl virus and a DNA-beta satellite component. This is the first experimental demonstration of Koch's postulates using cloned DNA molecules associated with chilli leaf curl disease.
authors.affiliation: Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
authors.forename: B
authors.initials: B
authors.lastname: Chattopadhyay
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.forename: T
authors.initials: T
authors.lastname: Yadav
authors.forename: C M
authors.initials: CM
authors.lastname: Fauquet
authors.forename: N B
authors.initials: NB
authors.lastname: Sarin
authors.forename: S
authors.initials: S
authors.lastname: Chakraborty
mesh_terms: D001483:Base Sequence; D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D002212:Capsicum / Q000821:virology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016415:Sequence Alignment
chemical_list: D004279:DNA, Viral