title: [Separation of water-soluble active components in Salvia miltiorrhiza bge. f. alba using capillary zone electrophoresis].
abstract: To establish a high performance capillary zone electrophoretic method for the separation of water-soluble active components, protocatechuic aldehyde (PAH), danshensu (DSS), and protocatechuic acid (PA), in Salvia miltiorrhiza bge. f. alba, many factors that can affect the resolution and the migration time, including the concentration of phosphate-borax buffer, pH value, separation voltage and detection wavelength were investigated. The optimized conditions were as follows: uncoated capillary column, 75 microm x 60.2 cm (effective length of 50 cm); column temperature, 25 degrees C; detection wavelength of the photo-diode-array detector, 210 nm; injection, 3.45 kPa x 4 s; separation voltage, 27.5 kV; running buffer, 5 mmol/L borax (pH 7.5). Under the optimized conditions, good separation of PAH, DSS and PA can be achieved in less than 8 min.
authors.affiliation: Taishan Medical University, Taian 271000, China. cuiyingjie77@163.com
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Cui
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.forename: Weifeng
authors.initials: W
authors.lastname: Shi
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Yang
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhao
authors.forename: Zuoli
authors.initials: Z
authors.lastname: Xia
mesh_terms: D001547:Benzaldehydes / Q000737:chemistry / Q000302:isolation & purification*; D002396:Catechols / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000737:chemistry / Q000302:isolation & purification*; D007773:Lactates / Q000737:chemistry / Q000302:isolation & purification*; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D001547:Benzaldehydes; D002396:Catechols; D062385:Hydroxybenzoates; D007773:Lactates; D014867:Water; C009091:protocatechuic acid; C035055:3,4-dihydroxyphenyllactic acid; C005581:protocatechualdehyde