title: [Regulatory effect of FK506 on the expression of CD152 and PD-1 in the liver allo-recipients].
abstract: OBJECTIVE
  To dynamically observe the expression of CTLA-4/CD152 and PD-1 on T cell surface in the peripheral blood of liver allo-recipients, and to explore the regulatory effect of FK506 on negative costimulatory molecules.
  
  
  METHODS
  The blood concentration of FK506 was measured by enzyme-multiplied immunoassay technique. Flow cytometry (FCM) was used to determine the expression of T cell subsets and CD152, PD-1 on T cell surface in the peripheral blood.
  
  
  RESULTS
  There was no significant difference in blood concentration of FK506 between each group (P > 0.05). With the progression of treatment, the frequency of CD4(+) T cells gradually decreased and was obviously lower than that in health control until the third month (P < 005). The expression of CD4(+) T cells in each treatment group was significantly lower than that in disease control group (P < 0.05). And the frequency of CD8(+) T cells in each treatment group was obviously higher than that in disease control group (P < 0.05). After liver transplantation, the expression of CD152 on CD4(+) and CD8(+) T cells was higher than that in health control group (P < 0.05), and the expression in the second week and first month was higher than that in disease control group (P < 0.05). The frequencies of CD4(+) CD152(+) T cells in the second and third month were lower than that in the second week and first month (P < 0.05). The frequency of CD8(+) CD152(+) T cells in the third month was lower than that in the second week and first month (P < 0.05). After liver transplantation, the expression of PD-1 on T cell subsets had the tendency of advancing. Its expression on CD4(+) T cells was significantly higher than that in health control group from the second week (P < 0.05), and the expression on CD8(+) T cells increased obviously from the first month compared with that in disease control group (P < 0.05).
  
  
  CONCLUSIONS
  FK506 could up-regulate the expression of negative costimulatory molecules CD152 and PD-1 on T cell surface and inhibit the proliferation and activation of effector T cells, which may contribute to maintain the survival and homeostasis of allo-recipients.
authors.affiliation: Division of Clinical Immunological Laboratory, West China Hospital of Sichuan University, Chengdu 610041, China. polarbearli@163.com
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Yang-juan
authors.initials: YJ
authors.lastname: Bai
authors.forename: Lan-lan
authors.initials: LL
authors.lastname: Wang
authors.forename: Hong-gang
authors.initials: HG
authors.lastname: Wei
authors.forename: Bei
authors.initials: B
authors.lastname: Cai
authors.forename: Lü-nan
authors.initials: LN
authors.lastname: Yan
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D060908:CTLA-4 Antigen; D049109:Cell Proliferation; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008099:Liver / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology*; D061026:Programmed Cell Death 1 Receptor; D013601:T-Lymphocytes; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D016559:Tacrolimus; D023001:Transplantation Tolerance / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C556707:Ctla4 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor; D007371:Interferon-gamma; D016559:Tacrolimus