title: Up-regulation of genes related to the ubiquitin-proteasome system in the brown adipose tissue of 24-h-fasted rats.
abstract: The functional balance between brown adipose tissue (BAT) and white adipose tissue (WAT) is important for metabolic homeostasis. We compared the effects of fasting on the gene expression profiles in BAT, WAT and liver by using a DNA microarray analysis. Tissues were obtained from rats that had been fed or fasted for 24 h. Taking the false discovery rate into account, we extracted the top 1,000 genes that had been differentially expressed between the fed and fasted rats. In all three tissues, a Gene Ontology analysis revealed that the lipid and protein biosynthesis-related genes had been markedly down-regulated. The whole-body fuel shift from glucose to triacylglycerol and the induction of autophagy were also observed. There was marked up-regulation of genes in the 'protein ubiquitination' category particularly in BAT of the fasted rats, suggesting that the ubiquitin-proteasome system was involved in saving energy as an adaptation to food shortage.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan. yunakai@iu.a.u-tokyo.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nakai
authors.forename: Hiroko
authors.initials: H
authors.lastname: Hashida
authors.forename: Koji
authors.initials: K
authors.lastname: Kadota
authors.forename: Michiko
authors.initials: M
authors.lastname: Minami
authors.forename: Kentaro
authors.initials: K
authors.lastname: Shimizu
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsumoto
authors.forename: Hisanori
authors.initials: H
authors.lastname: Kato
authors.forename: Keiko
authors.initials: K
authors.lastname: Abe
mesh_terms: D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002450:Cell Communication / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D004435:Eating; D005215:Fasting / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D007328:Insulin / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D001786:Blood Glucose; D007328:Insulin; D044767:Ubiquitin-Protein Ligases