Meal-induced compositional changes in blood and saliva in persons with bulimia nervosa.
BACKGROUND
Binge eating episodes in persons with bulimia nervosa may to some extent be a result of disturbed sensations of hunger and satiety. It has been hypothesized that abnormal appetite sensations may be due to bulimia nervosa-related alterations in the release of hormones that are known to be involved in the physiologic regulation of appetite and metabolism.


OBJECTIVE
The objective was to investigate whether circulating concentrations of the appetite-regulating peptides leptin and ghrelin and markers of metabolism (glucose and insulin) are different in persons with bulimia nervosa than in controls before and after intake of a meal and whether these changes may be reflected in saliva.


METHODS
Twenty women with bulimia nervosa and 20 age- and sex-matched healthy controls participated. After an overnight fast, the subjects ate a standardized carbohydrate-rich breakfast. Whole saliva and blood were collected, and visual analogue scales for hunger and satiety were completed once before and continuously for 5 h after the breakfast.


RESULTS
A lower pre- and postprandial whole saliva flow rate was found in subjects with bulimia nervosa, which might have been attributable to a concomitant intake of potentially xerogenic medication. Subjects with bulimia nervosa experienced reduced hunger, which could not be explained by pre- or postprandial alterations in circulating ghrelin, leptin, insulin, or glucose concentrations.


CONCLUSIONS
There were no apparent differences in the composition of blood and saliva between bulimia nervosa and control subjects, and meal-induced compositional changes in blood were not directly mirrored in saliva composition.
Dynesen
Anja W
AW
Department of Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. awd@odont.ku.dk
Bardow
Allan
A
Astrup
Arne
A
Petersson
Birgit
B
Holst
Jens J
JJ
Nauntofte
Birgitte
B
D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000032:analysis / Q000378:metabolism; D052018:Bulimia Nervosa / Q000097:blood* / Q000503:physiopathology*; D016022:Case-Control Studies; D004435:Eating / Q000502:physiology*; D005260:Female; D054439:Ghrelin / Q000032:analysis / Q000097:blood; D006801:Humans; D006815:Hunger / Q000502:physiology; D007328:Insulin / Q000032:analysis / Q000097:blood; D020738:Leptin / Q000032:analysis / Q000378:metabolism; D019518:Postprandial Period / Q000502:physiology; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D012528:Satiety Response / Q000502:physiology; D014987:Xerostomia / Q000453:epidemiology*
D001786:Blood Glucose; D054439:Ghrelin; D007328:Insulin; D020738:Leptin