Preventive effect of ultraviolet radiation on murine chronic sclerodermatous graft-versus-host disease.
Although previous studies have demonstrated the efficient modulatory effects of ultraviolet radiation B (UVB) on cutaneous graft-versus-host disease (GVHD), most animal research on GVHD has been performed in murine models of acute GVHD. Here, we studied the preventive effect of UVB radiation on the occurrence of chronic sclerodermatous (Scl) GVHD in a murine model. Scl GVHD was induced by transplanting lethally irradiated BALB/c mice with B10.D2 bone marrow and spleen cells. Recipient mice were exposed to UVB before or after bone marrow and spleen cell infusion. Histological and clinical evaluation of GVHD was performed, in association with the characterization of epidermal Langerhans cells. UVB irradiation of recipients after, and more remarkably before, transplantation induced a decrease of Scl GVHD severity associated with epidermal Langerhans cells depletion. We conclude that UVB irradiation of recipient before or after transplantation has a preventive effect on cutaneous Scl GVHD and may represent an effective strategy for prevention of Scl GVHD.
Mermet
Isabelle
I
Service de Dermatologie, Université et Centre Hospitalier Universitaire de Franche Comté, Besançon, France.
Kleinclauss
François
F
Marandin
Aliette
A
Guérrini
Jean Sébastien
JS
Angonin
Régis
R
Tiberghien
Pierre
P
Saas
Philippe
P
Aubin
François
F
D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000473:pathology*; D004195:Disease Models, Animal; D006086:Graft vs Host Disease / Q000276:immunology / Q000473:pathology; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000473:pathology / Q000528:radiation effects; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D045745:Scleroderma, Limited / Q000532:radiotherapy* / Q000601:surgery*; D013154:Spleen / Q000637:transplantation; D014184:Transplantation, Homologous / Q000276:immunology* / Q000473:pathology*; D014466:Ultraviolet Rays