title: Differentially expressed genes of Acanthamoeba castellanii during encystation.
abstract: To examine the expressed gene profile during encystation of Acanthamoeba castellanii Castellani, we used differentially expressed gene (DGE) screening by RT-PCR with 20 sets of random primers. From this analysis, we found that approximately 16 genes showed upregulation during encystation. We chose 6 genes, which had relatively higher expression levels, for further investigation. Based on homology search in database, DEG2 showed 55% of similarity with xylose isomerase, DEG9 showed 37% of similarity with Na P-type ATPase, and DEG14 showed 77% of similarity with subtilisin-like serine proteinase. DEG3 and DEG26 were identified as hypothetical proteins and DEG25 exhibited no significant similarity to any known protein. Encystation of Acanthamoeba has been suggested to be a process to resist adverse environmental or nutritional conditions. Further characterization studies of these genes may provide us with more information on the encystation mechanism of Acanthamoeba.
authors.affiliation: Department of Parasitology, Kyungpook National University School of Medicine, Korea.
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Moon
authors.forename: Dong Il
authors.initials: DI
authors.lastname: Chung
authors.forename: Yeon Chul
authors.initials: YC
authors.lastname: Hong
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Kong
mesh_terms: D048248:Acanthamoeba castellanii / Q000235:genetics* / Q000254:growth & development*; D000595:Amino Acid Sequence; D000818:Animals; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation
chemical_list: D015800:Protozoan Proteins