title: [Molecular comparison of Legionella pneumophila serogroup 1 isolated in Tunisia].
abstract: Legionella pneumophila is a common cause of hospital and community-acquired pneumonia, being transmitted by inhalation of aqueous aerosols. Most outbreaks are linked to contaminated hot water systems and cooling towers. Our study was about the molecular typing of 35 strains of L. pneumophila including four clinical isolates and 31 environmental strains isolated from the distribution systems of 14 hotels. Among the clinical strains, two have the same pattern, however, all were different from the studied environmental strains. For the 31 environmental strains, ten patterns were obtained. Among which, a same pulsotype was found for four strains isolated from four different establishments. In addition, two different pulsotypes were found for strains isolated from the same establishment. The pulsed-field gel electrophoresis showed the existence of various patterns. Although cases of legionellosis were declared in these hotels, there are no epidemiological links between the clinical and environmental strains.
authors.affiliation: Laboratoire de microbiologie, hôpital Abderahman-Mami de pneumologie, Ariana, Tunis 2080, Tunisie. emna.mehiri@tunet.tn
authors.forename: E
authors.initials: E
authors.lastname: Mehiri-Zghal
authors.forename: L
authors.initials: L
authors.lastname: Essalah
authors.forename: A
authors.initials: A
authors.lastname: Ghariani
authors.forename: W
authors.initials: W
authors.lastname: Mahjoubi
authors.forename: M
authors.initials: M
authors.lastname: Reyrolle
authors.forename: H
authors.initials: H
authors.lastname: Meugnier
authors.forename: F
authors.initials: F
authors.lastname: Forey
authors.forename: S
authors.initials: S
authors.lastname: Jarraud
authors.forename: J
authors.initials: J
authors.lastname: Freney
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: L
authors.initials: L
authors.lastname: Slim-Saidi
mesh_terms: D000328:Adult; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004866:Equipment Contamination; D006801:Humans; D007841:Latex Fixation Tests; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D011633:Public Facilities; D015183:Restriction Mapping; D012498:Sanitary Engineering; D012703:Serotyping; D014416:Tunisia / Q000453:epidemiology; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial