High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents.
A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.
Polyak
Boris
B
Department of Surgery, Drexel University College of Medicine, Philadelphia, PA 19102, USA.
Fishbein
Ilia
I
Chorny
Michael
M
Alferiev
Ivan
I
Williams
Darryl
D
Yellen
Ben
B
Friedman
Gary
G
Levy
Robert J
RJ
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000473:pathology; D001672:Biocompatible Materials / Q000737:chemistry; D002339:Carotid Arteries / Q000473:pathology; D002417:Cattle; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism*; D015316:Genetic Therapy / Q000379:methods; D008280:Magnetics; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D036103:Nanotechnology / Q000379:methods; D011108:Polymers / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013232:Steel / Q000737:chemistry*; D015607:Stents
D001672:Biocompatible Materials; D011108:Polymers; D013232:Steel