title: Mouse sectioned muscle regenerates following auto-grafting with muscle fragments: a new muscle precursor cells transfer?
abstract: It was discovered fifty years ago that a minced skeletal muscle replaced in its bed is able to regenerate. This regeneration is due to the presence of quiescent muscle precursor cells so-called satellite cells in the adult muscle which proliferate and fuse to regenerate new centronucleated fibres when the muscle is damaged. These observations open therapeutic perspectives and, in this study, we attempted to test in the mouse whether fragments of minced muscle regenerate new fibres to fill the gap resulting from the trans-section and retraction of the extensor digitorum longus muscle (EDL). When untreated this gap never regenerates. In agreement with Studitsky, we observed that a minced EDL replaced in its bed regenerates fibres that are spatially disorganised. Minced fragments of abdominus rectus muscle placed in the gap resulting of the trans-section of the EDL regenerate muscle fibres in the gap with a better organisation that in the whole minced muscle. These results could have putative clinical applications, for instance in the prevention of incontinence following prostatectomy which implies removal excision of a large part of the striated urethral sphincter.
authors.affiliation: Université Pierre et Marie Curie-Paris 6, Faculté de Médecine, Atelier de régénération neuromusculaire, 27 rue Chaligny, 75012 Paris, France. anne-sophie.bierinx@chusa.jussieu.fr
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Biérinx
authors.forename: Alain
authors.initials: A
authors.lastname: Sebille
mesh_terms: D000818:Animals; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000293:injuries* / Q000637:transplantation*; D012038:Regeneration / Q000502:physiology*; D032496:Satellite Cells, Skeletal Muscle / Q000502:physiology*; D014182:Transplantation, Autologous / Q000379:methods