Mechanistic studies of fluorescent sensors for the detection of reactive oxygen species.
Two new sensors for the detection of reactive oxygen species have been synthesized and characterized; they contain the 4-amino-7-nitrobenzofurazan (ABF) moiety covalently tethered to a phenol. Comparison of sensors ABFhd and dABFhd (the latter containing two ortho-methyl groups) shows that introduction of steric bulk leads to an improvement of fluorescent sensor performance, thus confirming our previous predictions based on computational chemistry. ABFhd and dABFhd are new tools for biological applications involving reactive oxygen species, in particular oxygen-centered radicals.
Heyne
Belinda
B
University of Ottawa, Department of Chemistry, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada.
Ahmed
Sara
S
Scaiano
J C
JC
D001583:Benzoxazoles / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D017382:Reactive Oxygen Species / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013997:Time Factors
C527573:4-amino-7-nitrobenzofurazan; D001583:Benzoxazoles; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species