[Clinical study on effect of Erzhi Tiangui Granule in improving the quality of oocytes and leukemia inhibitory factor in follicular fluid of women undergoing in vitro fertilization and embryo transfer].
OBJECTIVE
To investigate the effects of Erzhi Tiangui Granule (ETG) on the concentration of leukemia inhibitory factor (LIF) in follicular fluid (FF) and quality of oocytes/embryos after in vitro fertilization and embryo transfer (IVF-ET).


METHODS
Women undergoing IVF-ET were randomly assigned to two groups: the treated group (42 cases) treated with ETG combined with Western medicine and the control group (38 cases) treated with Western medicine alone. Syndrome of Shen-deficiency, amount of oocytes, rates of mature oocytes, fertilization, cleavage, high-quality embryos and pregnancy, as well as LIF level in FF were observed and compared between the two groups. The relationship of LIF level with mature oocyte rate and high-quality embryo rate was analyzed, respectively.


RESULTS
In the treated group after treatment, the syndrome of Shen-deficiency and the above-mentioned indexes tested were improved better than those in the control group, respectively. Correlation analysis showed that the LIF level in FF was positively correlated with the rate of mature oocyte, high-quality embryo and the outcome of pregnancy.


CONCLUSIONS
ETG can distinctively increase the amount of oocyte, elevate the quality of embryo and raise the successful rate of IVF-ET. The mechanism maybe correlated to the increase of LIF level in FF and the activating of microenvironment for its full expression.
Lian
Fang
F
Affiliated Hospital of Shandong University of TCM, Jinan. f_lian@163.com
Teng
Yi-li
YL
Zhang
Jian-wei
JW
D000328:Adult; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000627:therapeutic use; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000188:drug therapy*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D009865:Oocytes / Q000166:cytology; D008517:Phytotherapy; D016711:Yang Deficiency / Q000188:drug therapy
D004365:Drugs, Chinese Herbal; D053605:Leukemia Inhibitory Factor; C533485:erzhi tiangui; D005640:Follicle Stimulating Hormone