In vitro differentiation of mesenchymal progenitor cells derived from porcine umbilical cord blood.
Mesenchymal stem/progenitor cells (MPCs) were isolated from porcine umbilical cord blood (UCB) and their morphology, proliferation, cell cycle status, cell-surface antigen profile and expression of hematopoietic cytokines were characterized. Their capacity to differentiate in vitro into osteocytes, adipocytes and chondrocytes was also evaluated. Primary cultures of adherent porcine MPCs (pMPCs) exhibited a typical fibroblast-like morphology with significant renewal capacity and proliferative ability. Subsequent robust cell growth was indicated by the high percentage of quiescent (G0/G1) cells. The cells expressed the mesenchymal surface markers, CD29, CD49b and CD105, but not the hematopoietic markers, CD45 and CD133 and synthesized hematopoietic cytokines. Over 21 days of induction, the cells differentiated into osteocytes adipocytes and chondrocytes. The expression of lineage specific genes was gradually upregulated during osteogenesis, adipogenesis and chondrogenesis. We conclude that porcine umbilical cord blood contains a population of MPCs capable of self-renewal and of differentiating in vitro into three classical mesenchymal lineages.
Kumar
Basavarajappa Mohana
BM
College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Korea.
Yoo
Jae-Gyu
JG
Ock
Sun-A
SA
Kim
Jung-Gon
JG
Song
Hye-Jin
HJ
Kang
Eun-Ju
EJ
Cho
Seong-Keun
SK
Lee
Sung-Lim
SL
Cho
Jae-Hyeon
JH
Balasubramanian
Sivasankaran
S
Rho
Gyu-Jin
GJ
D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology; D034421:Sus scrofa; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
D015703:Antigens, CD; D053773:Transforming Growth Factor beta1