title: Bile acid-induced lung injury in newborn infants: a bronchoalveolar lavage fluid study.
abstract: OBJECTIVE
  Neonatal respiratory distress syndrome is associated with intrahepatic cholestasis of pregnancy, and bile acids may play a major role in neonatal bile acid pneumonia. Our aim was to demonstrate the bile acid presence in the bronchoalveolar lavage fluid of neonates affected by respiratory distress syndrome who were born from intrahepatic cholestasis of pregnancy and to investigate bile acid mechanisms of action in acute lung injury.
  
  
  METHODS
  In this prospective study, we enrolled 10 neonates delivered from intrahepatic cholestasis of pregnancy, affected by respiratory distress syndrome requiring mechanical ventilation (intrahepatic cholestasis of pregnancy group) and 2 control groups. The first group consisted of 20 infants with respiratory distress syndrome delivered from pregnancies without any sign of intrahepatic cholestasis of pregnancy (respiratory-distress-syndrome group), and the second group included 20 neonates with no lung disease who were ventilated for extrapulmonary reasons (no-lung-disease group). We measured bile acid and pH in the bronchoalveolar lavage fluid and serum bile acid levels in the first 24 hours of life.
  
  
  RESULTS
  Bile acids were measurable in the bronchoalveolar lavage fluid of all of the infants in the intrahepatic cholestasis of pregnancy group but were absent in the 2 control groups. Bronchoalveolar lavage fluid pH was not different among the 3 groups. Infants in the intrahepatic-cholestasis-of-pregnancy group had significantly higher serum bile acid levels compared with those in both of the control groups.
  
  
  CONCLUSIONS
  Bile acids are detectable in the bronchoalveolar lavage fluid of newborns from intrahepatic cholestasis of pregnancy affected by respiratory distress syndrome. Elevated serum bile acid levels in these infants allow us to hypothesize that bile acid reaches the lung after an uptake from the circulation. These findings strongly support a role for bile acid in causing bile acid pneumonia.
authors.affiliation: Division of Neonatology, Department of Pediatrics, University Hospital A. Gemelli, Catholic University of the Sacred Heart, Rome, Italy. enrizecca@rm.unicatt.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Zecca
authors.forename: Daniele
authors.initials: D
authors.lastname: De Luca
authors.forename: Silvia
authors.initials: S
authors.lastname: Baroni
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vento
authors.forename: Eloisa
authors.initials: E
authors.lastname: Tiberi
authors.forename: Costantino
authors.initials: C
authors.lastname: Romagnoli
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001647:Bile Acids and Salts / Q000009:adverse effects*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D016022:Case-Control Studies; D002780:Cholestasis, Intrahepatic / Q000150:complications* / Q000175:diagnosis; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015994:Incidence; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis*; D011256:Pregnancy Outcome; D011336:Probability; D011446:Prospective Studies; D012016:Reference Values; D012121:Respiration, Artificial; D012127:Respiratory Distress Syndrome, Newborn / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology*; D018570:Risk Assessment; D016019:Survival Analysis
chemical_list: D001647:Bile Acids and Salts