title: Interaction of Leishmania PTS2 receptor peroxin 7 with the glycosomal protein import machinery.
abstract: Leishmania proteins containing a peroxisomal targeting signal sequence 2 (PTS2) are selectively trafficked to the glycosome by associating with the peroxin 7 receptor protein (PEX7). The L. major PEX7 (LmPEX7) encodes a approximately 41 kDa protein that exhibits limited sequence identity with PEX7 homologues from other eukaryotic organisms. Functional characterization of recombinant and native LmPEX7 revealed that this receptor bound the PTS2 protein fructose-1,6-bisphosphate aldolase. Moreover, LmPEX7 also formed a tight association with the Leishmania PEX5, the cytosolic PTS1 receptor, and PEX14, a glycosomal peripheral membrane protein required for protein import into the glycosome. Mapping studies revealed that the Leishmania PEX7 binds to a domain on LdPEX5 encompassing residues 111-148 and to a site on LdPEX14 spanning residues 120-148. Finally, subcellular localization studies revealed that Leishmania PEX7 has a dual distribution within the cytosolic compartment and glycosomal lumen.
authors.affiliation: Institute of Parasitology, McGill University, MacDonald Campus, Ste. Anne de Bellevue, Quebec, Canada.
authors.forename: Ana Victoria C
authors.initials: AV
authors.lastname: Pilar
authors.forename: Kleber P
authors.initials: KP
authors.lastname: Madrid
authors.forename: Armando
authors.initials: A
authors.lastname: Jardim
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000737:chemistry; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D018320:Leishmania major / Q000378:metabolism*; D008830:Microbodies / Q000737:chemistry*; D018613:Microscopy, Confocal; D008954:Models, Biological; D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015800:Protozoan Proteins / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*
chemical_list: D000074437:Peroxisomal Targeting Signal 2 Receptor; D000074435:Peroxisome-Targeting Signal 1 Receptor; D015800:Protozoan Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D005634:Fructose-Bisphosphate Aldolase