title: A novel subcellular collagen organization process visualized by total internal reflection fluorescence microscopy.
abstract: The alpha1beta1 and alpha2beta1 integrins belong to a family of cell-surface molecules involved in structural contacts and signal-transduction events across the cell membrane. Employing two-dimensional substrates coated with fluorescently labeled type I collagen, we have discovered a novel subcellular matrix remodeling event that is particular to cells that express the fibrillar collagen receptor alpha2beta1. Cells expressing alpha1beta1 also perform this collagen organization process, but less proficiently. This work will provide a basis for subsequent studies of cell-mediated collagen fibril assembly.
authors.affiliation: Department of Pathology & Cell Biology, Columbia University, New York, New York 10032, USA.
authors.forename: Erik F
authors.initials: EF
authors.lastname: Young
authors.forename: Eugene E
authors.initials: EE
authors.lastname: Marcantonio
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D024042:Collagen Type I / Q000378:metabolism* / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism / Q000648:ultrastructure; D024022:Fibrillar Collagens / Q000378:metabolism* / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D022001:Focal Adhesions / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D039222:Integrin alpha1beta1 / Q000378:metabolism*; D038982:Integrin alpha2beta1 / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D041681:NIH 3T3 Cells; D011485:Protein Binding / Q000502:physiology
chemical_list: D024042:Collagen Type I; D024022:Fibrillar Collagens; D039222:Integrin alpha1beta1; D038982:Integrin alpha2beta1