Proteomic analysis for inhibitory effect of chitosan oligosaccharides on 3T3-L1 adipocyte differentiation.
In the present study, we performed a differential proteomic analysis using 2-DE combined with MS to clarify the molecular mechanism for the suppressive effect of chitosan oligosaccharides (CO) during differentiation of adipocyte 3T3-L1. Cell differentiation was significantly inhibited by CO at the concentration of 4 mg/mL. Protein mapping of adipocyte homogenates by 2-DE revealed that numerous protein spots were differentially altered in response to CO treatment. Out of 50 identified proteins showing significant alterations, six were up-regulated and 44 were down-regulated by CO treatment in comparison to control mature adipocytes. Among them, most of the proteins are associated with lipid metabolism, cytoskeleton, and redox regulation, in which the levels of farnesyl diphosphate synthetase (FDS), dedicator of cytokinesis 9 (DOCK9), and chloride intracellular channel 1 (CLIC1) were significantly reduced (>two-fold) with CO treatment. These results have not previously been examined in the context of adipogenesis, and thus can be used as novel biomarkers. Taken together with immunoblot analysis, it was concluded that the inhibitory effect of CO on adipocyte differentiation was mediated by C/EBPalpha and PPARgamma pathway through significant downregulations of important adipogenic molecules such as fatty acid binding protein and glucose transporter 4.
Rahman
Atiar
A
Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Republic of Korea.
Kumar
Suresh G
SG
Kim
Sang Woo
SW
Hwang
Hye Jin
HJ
Baek
Yu Mi
YM
Lee
Sung Hak
SH
Hwang
Hee Sun
HS
Shon
Yun Hee
YH
Nam
Kyung Soo
KS
Yun
Jong Won
JW
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D050156:Adipogenesis / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D048271:Chitosan / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D051379:Mice; D009844:Oligosaccharides / Q000494:pharmacology*; D047495:PPAR gamma / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction
D022763:CCAAT-Enhancer-Binding Protein-alpha; D009844:Oligosaccharides; D047495:PPAR gamma; D020543:Proteome; D048271:Chitosan