title: Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery.
abstract: Cationic polyorganophosphazene has shown the ability to deliver gene. To obtain more efficient transfection, His(Boc)-OMe bearing histidine moiety was introduced to synthesize a new derivative of cationic polyphosphazenes with another side group of 2-dimethylaminoethylamine (DMAEA). The poly(DMAEA/His(Boc)-OMe)phosphazene (PDHP) and DNA could self-assemble into nanoparticles with a size around 110 nm and zeta potential of +15 mV at the PDHP/DNA ratio of 10:1 (w/w). The maximum transfection efficiency of PDHP/DNA self-assembled nanoparticles (PHSNs) against 293 T cells was much higher than that of poly(di-2-dimethylaminoethylamine) phosphazenes (PDAP)/DNA self-assembled nanoparticles (PASNs) and PEI 25/DNA self-assembled nanoparticles (PESNs) at the polymer/DNA ratio of 10:1, but the cytotoxicity of PDHP assayed by MTT was much lower than that of PDAP and PEI 25. These results suggested that PDHP could be a good candidate with high transfection efficiency and low cytotoxicity for gene delivery.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Fudan University, Shanghai, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
authors.forename: Shangwei
authors.initials: S
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.forename: Wangwen
authors.initials: W
authors.lastname: Gu
authors.forename: Lingli
authors.initials: L
authors.lastname: Chen
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Pei
authors.forename: Yaping
authors.initials: Y
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage*; D018014:Gene Transfer Techniques; D006801:Humans; D053758:Nanoparticles; D009943:Organophosphorus Compounds / Q000008:administration & dosage*; D010316:Particle Size; D011108:Polymers / Q000008:administration & dosage*; D014162:Transfection
chemical_list: D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene); D004247:DNA