Conditional forebrain deletion of the L-type calcium channel Ca V 1.2 disrupts remote spatial memories in mice.
To determine whether L-type voltage-gated calcium channels (L-VGCCs) are required for remote memory consolidation, we generated conditional knockout mice in which the L-VGCC isoform Ca(V)1.2 was postnatally deleted in the hippocampus and cortex. In the Morris water maze, both Ca(V)1.2 conditional knockout mice (Ca(V)1.2(cKO)) and control littermates displayed a marked decrease in escape latencies and performed equally well on probe trials administered during training. In distinct contrast to their performance during training, Ca(V)1.2(cKO) mice exhibited significant impairments in spatial memory when examined 30 d after training, suggesting that Ca(V)1.2 plays a critical role in consolidation of remote spatial memories.
White
Jessica A
JA
Molecular & Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan 48109-0069, USA.
McKinney
Brandon C
BC
John
Manorama C
MC
Powers
Patricia A
PA
Kamp
Timothy J
TJ
Murphy
Geoffrey G
GG
D000818:Animals; D020746:Calcium Channels, L-Type / Q000172:deficiency* / Q000235:genetics*; D002462:Cell Membrane / Q000502:physiology; D017931:DNA Primers; D004195:Disease Models, Animal; D017353:Gene Deletion; D008569:Memory Disorders / Q000235:genetics* / Q000503:physiopathology; D051379:Mice; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction; D016548:Prosencephalon / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013028:Space Perception; D013997:Time Factors
C514350:CACNA1C protein, mouse; D020746:Calcium Channels, L-Type; D017931:DNA Primers