Anti-plasmodial activity of Ailanthus excelsa.
The anti-plasmodial activity of Ailanthus excelsa stem bark was investigated. The methanolic extract inhibited in vitro growth of chloroquine-sensitive (D10) and resistant strains (W2) of Plasmodium falciparum (IC50 4.6 and 2.8 microg/ml, respectively). The effect was retained in the chloroform fraction (3.1 and 2.1 microg/ml, respectively). The anti-plasmodial activity could be ascribed to the impairment of haemoglobin degradation through the inhibition of plasmepsin II activity (IC50 of 13.43+/-1.74 microg/ml) and of the haem detoxification to haemozoin.
Dell'Agli
Mario
M
Department of Pharmacological Sciences, University of Milan, Milan, Italy. mario.dellagli@unimi.it
Galli
Germana V
GV
Parapini
Silvia
S
Basilico
Nicoletta
N
Taramelli
Donatella
D
Said
Ataa
A
Rashed
Khaled
K
Bosisio
Enrica
E
D032268:Ailanthus; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000037:antagonists & inhibitors*; D002738:Chloroquine; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology; D006420:Hemeproteins / Q000096:biosynthesis; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D010963:Plasmodium falciparum / Q000187:drug effects*; D015800:Protozoan Proteins
D006420:Hemeproteins; D010936:Plant Extracts; D015800:Protozoan Proteins; C020753:hemozoin; D002738:Chloroquine; D016282:Aspartic Acid Endopeptidases; C089643:plasmepsin; C100637:plasmepsin II