Antioxidant stress is promoted by nano-anatase in spinach chloroplasts under UV-B radiation.
A proven photocatalyst, titanium dioxide in the form of nano-anatase, is capable of undergoing electron transfer reactions under light. In previous studies, we had proven that nano-anatase could absorb ultraviolet light (UV-B) and convert light energy to stable chemistry energy finally via electron transport in spinach chloroplasts. The mechanisms by which nano-anatase promotes antioxidant stress in spinach chloroplasts under UV-B radiation are still not clearly understood. In the present paper, we investigate the effects of nano-anatase on the antioxidant stress in spinach chloroplasts under UV-B radiation. The results showed that nano-anatase treatment could significantly decrease accumulation of superoxide radicals O2.-, hydrogen peroxide (H2O2), and malonyldialdehyde (MDA) content, and increase activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and elevate evolution oxygen rate in spinach chloroplasts under UV-B radiation. Together, nano-anatase could decrease the oxidative stress to spinach chloroplast caused by UV-B radiation.
Lei
Zheng
Z
College of Life Sciences, Suzhou University, Suzhou, 215123, People's Republic of China.
Mingyu
Su
S
Xiao
Wu
W
Chao
Liu
L
Chunxiang
Qu
Q
Liang
Chen
C
Hao
Huang
H
Xiaoqing
Liu
L
Fashui
Hong
H
D000975:Antioxidants / Q000378:metabolism*; D060387:Ascorbate Peroxidases; D002736:Chloroplasts / Q000187:drug effects* / Q000528:radiation effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010544:Peroxidases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D018724:Spinacia oleracea; D014025:Titanium / Q000494:pharmacology*; D014466:Ultraviolet Rays
D000975:Antioxidants; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D014025:Titanium; D010544:Peroxidases; D060387:Ascorbate Peroxidases