PGD-derived human embryonic stem cell lines as a powerful tool for the study of human genetic disorders.
Human embryonic stem (ES) cells are derived from the inner cell mass (ICM) of blastocyst embryos. They are established from spare embryos that have been obtained by in vitro fertilization (IVF) and donated for research purposes. The ICM-derived cell lines have two unique properties, they can be propagated indefinitely in culture and have the potential to develop into practically any cell type in vitro and in vivo. Human embryonic stem (hES) cells carrying specific mutations can be used as a valuable tool for studying genetic disorders in human. One favorable approach to obtain such mutant ES cell lines is their derivation from affected preimplantation genetic diagnosed (PGD) embryos. This review focuses on the importance of deriving human ES cell lines from genetically abnormal embryos, especially in cases where no good cellular and/or animal models exist.
Ben-Yosef
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Racine IVF Unit, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center affiliated to the Sackler Faculty of Medicine, 6 Weizman Street, Tel Aviv University, Tel-Aviv 64239, Israel. dalitb@tasmc.health.gov.il
Malcov
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Eiges
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D002460:Cell Line; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D053595:Embryonic Stem Cells; D030342:Genetic Diseases, Inborn / Q000175:diagnosis / Q000503:physiopathology; D006801:Humans; D019836:Preimplantation Diagnosis / Q000379:methods*