Prevention of postoperative adhesions by a novel honeycomb-patterned poly(lactide) film in a rat experimental model.
Intraperitoneal adhesion is a serious problem concerning abdominal surgery. This study evaluated the performance of a honeycomb-patterned poly(lactide) (HCPLA) film as a physical barrier for preventing postoperative adhesion. HCPLA films were prepared using dioleoylphosphatidylethanolamine (DOPE) or a copolymer of dodecylacrylamide and omega-carboxyhexylacrylamide (CAP) as a surfactant (HCPLA-DOPE and HCPLA-CAP, respectively). In an in vivo adhesion prevention experiment, male Sprague-Dawley rats underwent standard cecum abrasion before midline laparotomy. We placed 2 cm x 2 cm HCPLA and flat films on the gliding interfaces; untreated rats formed the control group. After 1 week, adhesion was scored from 0 to 4. No significant difference was observed in the scores among groups, but macroscopic differences in adhesion prevention were observed. The adhesive strength of HCPLA-DOPE (18.1 +/- 1.2 g) to skinless chicken breast was significantly higher than that of the flat film (15.2 +/- 0.8 g, p < 0.05). Further, the adhesion score after 1 week for the HCPLA-DOPE group (1.6 +/- 0.2) was significantly lower than that for the control group (3.0 +/- 0.3, p < 0.05) but comparable to that for the Seprafilm group (1.4 +/- 0.3). These results demonstrated the potential of HCPLA-DOPE as a physical barrier for preventing postoperative adhesion.
Fukuhira
Yukako
Y
Department of Tissue Engineering Development, Innovation Research Institute, Teijin Limited, Hino, Tokyo 191-8512, Japan. y.fukuhira@teijin.co.jp
Ito
Masaya
M
Kaneko
Hiroaki
H
Sumi
Yoshihiko
Y
Tanaka
Masaru
M
Yamamoto
Sadaaki
S
Shimomura
Masatsugu
M
D034861:Abdominal Wall; D000268:Adhesiveness; D000818:Animals; D008297:Male; D008422:Materials Testing; D023421:Models, Animal; D011091:Polyesters / Q000627:therapeutic use*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013526:Surgical Mesh; D000267:Tissue Adhesions / Q000517:prevention & control*
D011091:Polyesters; C033616:poly(lactide)