Near-infrared fluorescent labeled peptosome for application to cancer imaging.
Nonionic amphiphilic copolypeptides, which were composed of hydrophilic poly(sarcosine) and hydrophobic poly(gamma-methyl L-glutamate) blocks, were synthesized with varying chain lengths of the blocks. The polypeptides having a suitable hydrophilic and hydrophobic balance were found to form vesicular assemblies of 100 nm size in buffer, which was evidenced by the TEM observation, the DLS analysis, and the encapsulation experiment. The genuine peptide vesicles, peptosomes, were labeled with a near-infrared fluorescence (NIRF) probe. In vivo retention in blood experiment showed long circulation of the peptosome in rat blood as stable as the PEGylated liposome. NIRF imaging of a small cancer on mouse by using the peptosome as a nanocarrier was successful due to the EPR effect of the peptosome. Peptosome is shown here as a novel excellent nanocarrier for molecular imaging.
Tanisaka
Hiroki
H
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
Kizaka-Kondoh
Shinae
S
Makino
Akira
A
Tanaka
Shotaro
S
Hiraoka
Masahiro
M
Kimura
Shunsaku
S
D000818:Animals; D002942:Circular Dichroism; D004337:Drug Carriers / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D007259:Infrared Rays; D008297:Male; D051379:Mice; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry; D009369:Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D010539:Permeability; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D051381:Rats; D012521:Sarcosine / Q000031:analogs & derivatives / Q000737:chemistry
D004337:Drug Carriers; D005456:Fluorescent Dyes; D008823:Micelles; D010455:Peptides; C030315:poly-gamma-methylglutamate; D011099:Polyglutamic Acid; C015475:polysarcosine; D016650:Fluorescein-5-isothiocyanate; D012521:Sarcosine