Multilocus phylogenetic analysis of Cryptosporidium andersoni (Apicomplexa) isolated from a bactrian camel (Camelus bactrianus) in China.
This is the first report of cryptosporidiosis in a bactrian camel (Camelus bactrianus) in China. Two Cryptosporidium isolates derived from the same bactrian camel (3-year-old) in November 2005 and April 2006 were characterized using sequence and phylogenetic analysis of the small-subunit rRNA (18S rRNA), 70-kDa heat shock protein (HSP70), actin and Cryptosporidium oocyst wall protein (COWP) genes. The sequences of the 18S rRNA and COWP were identical to all other Cryptosporidium andersoni isolates although minor differences were noticed between the isolates and the USA isolate at the actin locus (99.2% of similarity). The sequence of the HSP70 was identical to the Japanese C. andersoni isolate, with a minor difference from the Australian C. andersoni isolate (99.7% of similarity). Cross-transmission studies demonstrated that the C. andersoni isolates did not infect immunosuppressed or immunocompetent Kun-ming mice, severe combined immunodeficiency mice, and immunosuppressed or immunocompetent calves. Among the C. andersoni isolates reported so far, only isolates from Japan could infect SCID mice. Thus, the C. andersoni isolates from the bactrian camel were biologically similar to most bovine C. andersoni isolates characterized so far, but are different from bovine isolates from Japan.
Wang
Rongjun
R
The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, the People's Republic of China.
Zhang
Longxian
L
Ning
Changshen
C
Feng
Yaoyu
Y
Jian
Fuchun
F
Xiao
Lihua
L
Lu
Biao
B
Ai
Weichang
W
Dong
Heping
H
D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000469:parasitology*; D002417:Cattle; D002681:China; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D016054:DNA, Protozoan / Q000032:analysis; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000199:Actins; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S; C106305:oocyst wall protein, Cryptosporidium