Serum diagnosis of diffuse large B-cell lymphomas and further identification of response to therapy using SELDI-TOF-MS and tree analysis patterning.
BACKGROUND
Currently, there are no satisfactory biomarkers available to screen for diffuse large B cell lymphoma (DLBCL) or to identify patients who do not benefit from standard anti-cancer therapies. In this study, we used serum proteomic mass spectra to identify potential serum biomarkers and biomarker patterns for detecting DLBCL and patient responses to therapy.


METHODS
The proteomic spectra of crude sera from 132 patients with DLBCL and 75 controls were performed by SELDI-TOF-MS and analyzed by Biomarker Patterns Software.


RESULTS
Nine peaks were considered as potential DLBCL discriminatory biomarkers. Four peaks were considered as biomarkers for predicting the patient response to standard therapy. The proteomic patterns achieved a sensitivity of 94% and a specificity of 94% for detecting DLBCL samples in the test set of 85 samples, and achieved a sensitivity of 94% and a specificity of 92% for detecting poor prognosis patients in the test set of 66 samples.


CONCLUSIONS
These proteomic patterns and potential biomarkers are hoped to be useful in clinical applications for detecting DLBCL patients and predicting the response to therapy.
Zhang
Xing
X
Department of Medical Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China. xingzhangg@hotmail.com
Wang
Bo
B
Zhang
Xiao-shi
XS
Li
Zhi-ming
ZM
Guan
Zhong-zhen
ZZ
Jiang
Wen-qi
WQ
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000473:pathology; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis; D003131:Combined Modality Therapy; D003663:Decision Trees; D005819:Genetic Markers; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000097:blood* / Q000175:diagnosis / Q000628:therapy*; D008875:Middle Aged; D015233:Models, Statistical; D009363:Neoplasm Proteins / Q000097:blood*; D011237:Predictive Value of Tests; D011379:Prognosis; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics / Q000379:methods*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016896:Treatment Outcome
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D005819:Genetic Markers; D009363:Neoplasm Proteins