In vitro antioxidant, antimutagenic and genoprotective activity of Rosa roxburghii fruit extract.
The antioxidant properties of the fruit of the Rosa roxburghii (RR) plant have been associated with several putative health promoting effects. The possible cytotoxic, mutagenic/antimutagenic and genotoxic effects of RR fruit extract were investigated. The effect on antioxidant status and protection against induced oxidative stress were also investigated using primary rat hepatocytes. A RR fruit extract containing 45 g/L total ascorbic acid and 65 g/L total polyphenols was used in this study. Dilutions up to 0.08% (v/v) increased significantly the antioxidant status in primary rat hepatocytes. The glutathione redox state was decreased with RR treatment but was increased in Chang liver cells and MT-2 lymphoblast. No cyto- or genotoxicity were observed at levels of up to 5% (v/v) of the fruit extract. In addition, a significant protection against t-BHP induced oxidative stress was observed in primary rat hepatocytes. The Ames test revealed no mutagenic activity using the Salmonella typhimurium strains TA98, TA100 and TA102. A significant antimutagenic effect of the extract was observed against the metabolic activated mutagens 2-acetylaminofluorene and aflatoxin B1 and to a lesser extent against methyl methanesulfonate. It is concluded that these results support the associated health promoting potential of Rosa Roxburghii fruit and in particular against oxidative stress.
van der Westhuizen
Francois H
FH
School for Biochemistry, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa. Francois.vanderWesthuizen@nwu.ac.za
van Rensburg
Catharina S Janse
CS
Rautenbach
George S
GS
Marnewick
Jeanine L
JL
Loots
Du Toit
du T
Huysamen
Cristal
C
Louw
Roan
R
Pretorius
Pieter J
PJ
Erasmus
Elardus
E
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D005638:Fruit / Q000737:chemistry; D005978:Glutathione / Q000032:analysis / Q000187:drug effects; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000187:drug effects; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D016587:Antimutagenic Agents; D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D020122:tert-Butylhydroperoxide; D005978:Glutathione