[Improvement of urinary d-glucaric acid assay and its application].
We propose an improved method to measure urinary D-glucaric acid (GA), which might be of value as an indirect index of the activity of cytochrome P450 (CYP). This method was about 20 times more sensitive than existing methods. Beer's law was obeyed in the concentration range 5.5-66 ng ml(-1) for GA, with the effective molar absorptivity at 533 nm and the relative standard deviation being 9.1 x 10(5) dm(3) mol(-1) cm(-1) and 0.69% (n = 6), respectively. In addition, we introduced the correction value {GA/Cr ratio x10} of urinary GA by measuring urinary creatinine (Cr) at the same time. Based on the proposed method, the GA and Cr values in spot urines of healthy persons and cancer patients were subsequently measured and the correction values of both groups subjected to comparison. As a result, a statistically significant difference was recognized between the two groups.
Hasegawa
Kohei
K
Department of Clinical Chemistry, Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki City, Japan.
Shimidzu
Kumiyo
K
Morimoto
Shigehumi
S
Hachino
Yoshimi
Y
Hasegawa
Kenji
K
Shono
Yoshiharu
Y
Horiuchi
Tetuya
T
Tabuse
Katsuyoshi
K
Masui
Yurina
Y
Yamaguchi
Takako
T
Fujita
Yoshikazu
Y
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000493:pharmacokinetics; D015415:Biomarkers / Q000652:urine; D003404:Creatinine / Q000652:urine; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004334:Drug Administration Schedule; D016903:Drug Monitoring; D005937:Glucaric Acid / Q000652:urine*; D006801:Humans; D008099:Liver / Q000201:enzymology; D009369:Neoplasms / Q000188:drug therapy / Q000652:urine
D000970:Antineoplastic Agents; D015415:Biomarkers; D003577:Cytochrome P-450 Enzyme System; D003404:Creatinine; D005937:Glucaric Acid