title: [Phylogeny relationship and molecular identification of ten Huperzia species (Huperziaceae) based on matK gene sequences].
abstract: OBJECTIVE
  To study the phylogeny relationship and molecular identification of 10 species from Huperzia (Huperziaceae) based on matK gene sequences data.
  
  
  METHODS
  Total DNA of nine species from Huperzia was extracted; matK gene sequence was amplified by PCR. PCR product was directly sequenced after purification.
  
  
  RESULTS
  The chloroplast matK gene nucleotide sequences from 9 species of Huperzia species were sequenced. The matK gene nucleotide sequences length was 1 589 bp. Analysis with Huperzia lucidula matK gene nucleotide sequences (download from GenBank) and taking Lycopodiella cernua as outgroup, Maximum Parsimony, Neighbor-Joining analyses and genetic distances were conducted using MEGA 3.1 software. 35 variable sites and 35 parsimony informative sites have been found. Pairwise genetic distances among 10 species of Huperzia was 1.59% - 0.25%.
  
  
  CONCLUSIONS
  The results were consistent with the taxonomy in morphological of Huperzia. But H. longipetiolata and H. serrata were resolved into in different clade. There are 19 different sites of matK gene sequences between H. longipetiolata and H. serrata, the genetic distances is 0.121%. It is suggested H. longipetiolata should be as an independent species.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai 200032, China.
authors.forename: Sheng-Guo
authors.initials: SG
authors.lastname: Ji
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Ke-Ke
authors.initials: KK
authors.lastname: Huo
mesh_terms: D018742:DNA, Chloroplast / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D004722:Endoribonucleases / Q000235:genetics*; D032501:Huperzia / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal / Q000145:classification / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018742:DNA, Chloroplast; D018744:DNA, Plant; D010940:Plant Proteins; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases