[An experimental study of the effect of burying testis in thigh pocket on spermatogenesis].
OBJECTIVE
To explore the effect of burying testis in thigh pocket on spermatogenesis.


METHODS
Guizhou miniature male pigs at child-bearing period were randomly divided to receive operation of scrotum incision and dissection with the testis burying in thigh pocket (experimental group) or without (control group). 3 months later, testis biopsy was performed on 2 pigs from each group for pathological examination. Then every male pig from both experimental (n = 6) and control group (n = 6) got a mating partner and lived together for 3 months. The fertility of the male pigs was observed. 6 months after operation, testis biopsy was performed again on all the animals from both the groups.


RESULTS
Both at 3 months and 6 months after operation, the pathological examination showed the spermatogenic cells of all stage in contorted seminiferous tubules markedly decreased with no mature sperm in experimental group, while normal spermatogenic cells with mature sperm in control group. After the male pigs lived with mating partners for 3 months, no female pigs staying with the experimental group became pregnant, but the male pigs in control group had a normal fertility.


CONCLUSIONS
Burying testis in thigh pocket impedes spermatogenesis in the miniature male pig. So burying testis in thigh pocket is not recommended for patients with scrotum skin defect who wish to remain fertile.
Wang
Da-li
DL
Department of Plastic Surgery, Affiliated Hospital, Zunyi, China.
Wang
Yu-ming
YM
Wei
Zai-rong
ZR
Zheng
Hong
H
Deng
Fei
F
Gao
Zhen-yu
ZY
D000818:Animals; D005260:Female; D005298:Fertility; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012611:Scrotum; D012867:Skin / Q000293:injuries; D013091:Spermatogenesis; D013552:Swine; D013556:Swine, Miniature; D013737:Testis / Q000502:physiology*; D013848:Thigh / Q000601:surgery*