[The effects of citalopram on proliferation and differentiation of neural precursor cells from embryonic rat hippocampus].
OBJECTIVE
To survey the effects of citalopram on proliferation and differentiation of neural precursor cells (NPCs) from embryonic rat hippocampus.


METHODS
Single cell suspensions were prepared from the hippocampus of 14.5 days embryos rat. After formation of the primary neurospheres, single cell clone was obtained with limited dilution method. Then the single cell clone spheres were digested and induced to proliferate and differentiate using non-serum medium and bovine serum. The effects of varied concentration citalopram on the proliferation and differentiation of NPCs were investigated by using mononuclear cell direct cytotoxicity (MTT) reduction assay and immunofluorescence method respectively.


RESULTS
MTT showed that citalopram (1.0 micromol/L) could increase the absorbance (A(570) nm) from 3 days to 7 days after intervention significantly (all P < 0.0001). Immunofluorescence showed that percentage of NeuN positive cells were 59.5% +/- 2.8% and 57.8% +/- 2.1% respectively after 7-day intervention of 1.0 micromol/L and 0.5 micromol/L citalopram, more than 47.3% +/- 4.3% of control group (all P < 0.05).


CONCLUSIONS
Citalopram promotes proliferation and differentiation of NPCs from embryonic rat hippocampus.
Wang
Shao-hua
SH
Neurological Department of the Affiliated Zhong Da Hospital of South East University, Nanjing 210009, China.
Zhang
Zhi-jun
ZJ
Guo
Yi-jing
YJ
Teng
Gao-jun
GJ
Chen
Bao-an
BA
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015283:Citalopram / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000166:cytology / Q000196:embryology; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D015283:Citalopram