title: Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy.
abstract: A scanning-less single-photon counting system for FLIM and fluorescence anisotropy wide-field imaging is described and characterized in this paper. The two polarizations of the fluorescence are divided by a Glan prism and acquired at the same time by the Q(A) detector. Fluorescence decay profiles can be reconstructed for any desired area up to each pixel and used to calculate time-resolved fluorescence anisotropy decays.
authors.affiliation: PPSM, Institut d'Alembert, ENS Cachan, CNRS, UniverSud, 61 av President Wilson, F-94230, Cachan, France.
authors.forename: J-A
authors.initials: JA
authors.lastname: Spitz
authors.forename: R
authors.initials: R
authors.lastname: Yasukuni
authors.forename: N
authors.initials: N
authors.lastname: Sandeau
authors.forename: M
authors.initials: M
authors.lastname: Takano
authors.forename: J-J
authors.initials: JJ
authors.lastname: Vachon
authors.forename: R
authors.initials: R
authors.lastname: Meallet-Renault
authors.forename: R B
authors.initials: RB
authors.lastname: Pansu
mesh_terms: D004396:Coloring Agents; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D010777:Photochemistry; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D012235:Rhodamines