[Experimental study of construction of tissue engineered bone ectopically by human bone marrow mesenchymal stem cells].
OBJECTIVE
To study the possibility and mechanism of construction of tissue engineered bone with human bone marrow mesenchymal stem cells (hBMSCs) as seeding cells and partially demineralized bone matrix (pDBM) as scaffold.


METHODS
hBMSCs are cultured and mutiplified. The 4th grade hBMSCs are seeded on the pDBM, the growth and adhesion of hBMSCs on pDBM are observed under scanning electro microscope. The adhesion efficiency is assessed. The complexes are implanted in the nude mice subcutaneously, the pDBM without cells as control. The grafts are taken out on the 8th and 12th week.


RESULTS
There is new bone formation on the 8th and 12th week in complex group. There is a layer of osteoblast like cells adhered on the surface of most of the new bone, which suggest the possibility of intramembranous ossification. There is no bone formation in control group.


CONCLUSIONS
Tissue engineered bone can be constructed with hBMScs and pDBM in vivo, and the mechanism of which could be intramembranous ossification.
Li
Dong
D
Plastic and Burn Surgery, Qilu Hospital, Shandong University, Jinan, China.
Liu
Xiang-dong
XD
Chai
Gang
G
Shu
Chao-feng
CF
Liu
Wei
W
Cui
Lei
L
Cao
Yi-lin
YL
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D001842:Bone and Bones; D002448:Cell Adhesion; D002454:Cell Differentiation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D023822:Tissue Engineering / Q000379:methods*