Homeostatic regulation of AMPA receptor expression at single hippocampal synapses.
Homeostatic synaptic response is an important measure in confining neuronal activity within a narrow physiological range. Whether or not homeostatic plasticity demonstrates synapse specificity, a key feature characteristic of Hebbian-type plasticity, is largely unknown. Here, we report that in cultured hippocampal neurons, alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid subtype glutamate receptor (AMPAR) accumulation is increased selectively in chronically inhibited single synapses, whereas the neighboring normal synapses remain unaffected. This synapse-specific homeostatic regulation depends on the disparity of synaptic activity and is mediated by GluR2-lacking AMPARs and PI3-kinase signaling. These results demonstrate the existence of synaptic specificity and the crucial role of AMPAR-gated calcium in homeostatic plasticity in central neurons.
Hou
Qingming
Q
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA.
Zhang
Dawei
D
Jarzylo
Larissa
L
Huganir
Richard L
RL
Man
Heng-Ye
HY
D000818:Animals; D004594:Electrophysiology / Q000379:methods; D019706:Excitatory Postsynaptic Potentials; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D017774:Long-Term Potentiation; D009474:Neurons / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying / Q000096:biosynthesis; D051381:Rats; D018091:Receptors, AMPA / Q000096:biosynthesis*; D015398:Signal Transduction; D013569:Synapses / Q000378:metabolism* / Q000502:physiology*; D014162:Transfection
C489461:Kir2.1 channel; D024661:Potassium Channels, Inwardly Rectifying; D018091:Receptors, AMPA; D019869:Phosphatidylinositol 3-Kinases