title: Ultrastructural localization of extranuclear progestin receptors relative to C1 neurons in the rostral ventrolateral medulla.
abstract: To better understand the role of progestins in the C1 area of the rostral ventrolateral medulla (RVLM), immunocytochemical localization of progestin receptors (PRs) was combined with tyrosine hydroxylase (TH) in single sections of RVLM from proestrus rat brains prepared for light and electron microscopy. By light microscopy, PR-immunoreactivity (-ir) was detected in a few nuclei that were interspersed between TH-labeled perikarya and dendrites. Electron microscopy revealed that PR-ir was in several extranuclear locations. The majority of PR-labeling was in non-TH immunoreactive axons (51+/-9%) near the plasma membrane. Additional dual labeling studies revealed that PR-immunoreactive axons could give rise to terminals containing the GABAergic marker GAD65. PR-ir also was found in non-neuronal processes (29+/-9%), some resembling astrocytes. Occasionally, PR-ir was in non-TH-labeled terminals (10+/-3%) affiliated with clusters of small synaptic vesicles, or in patches contained in the cytoplasm of dendrites (10+/-1%). These findings suggest that progestins can primarily modulate neurons in the C1 area of the RVLM by presynaptic mechanisms involving GABAergic transmission. Moreover, they suggest that PR activation may contribute to progestin's effects on arterial blood pressure during pregnancy as well as to sex differences in central cardiovascular regulation.
authors.affiliation: Division of Neurobiology, Department of Neurology and Neuroscience, Weill Cornell Medical College, 411 East 69th Street, New York, NY 10021, United States. tmilner@med.cornell.edu
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Milner
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Mitterling
authors.forename: Costantino
authors.initials: C
authors.lastname: Iadecola
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Waters
mesh_terms: D000818:Animals; D005260:Female; D005968:Glutamate Decarboxylase / Q000378:metabolism; D008526:Medulla Oblongata / Q000166:cytology*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D011980:Receptors, Progesterone; D014446:Tyrosine 3-Monooxygenase; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2