title: Temporal variation of dielectric properties of preserved blood.
abstract: Rabbit blood was preserved at 277 K in Alsever's solution for 37 days, and its dielectric permittivity was monitored in a frequency range from 0.05 to 110 MHz throughout the period. The relaxation time and Cole-Cole parameter of the interfacial polarization process for erythrocytes remained nearly constant during the first 20 days and then started to increase and decrease, respectively. On the other hand, the relaxation strength and the cell volume fraction continued to decrease for 37 days, but the decrease rates of both changed discontinuously on about the 20th day. Microscope observation showed that approximately 90% of the erythrocytes were spinous echinocytes at the beginning of preservation and started to be transformed into microspherocytes around the 20th day. Therefore, dielectric spectroscopy is a sensitive tool to monitor the deterioration of preserved blood accompanied by morphological transition of erythrocytes through the temporal variation of their dielectric properties.
authors.affiliation: Life Science Laboratory, Materials Laboratories, Sony Corporation, Sony Bioinformatics Center, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan. Yoshihito.Hayashi@jp.sony.com
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Hayashi
authors.forename: Ikuya
authors.initials: I
authors.lastname: Oshige
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Katsumoto
authors.forename: Shinji
authors.initials: S
authors.lastname: Omori
authors.forename: Akio
authors.initials: A
authors.lastname: Yasuda
authors.forename: Koji
authors.initials: K
authors.lastname: Asami
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D001790:Blood Physiological Phenomena; D001793:Blood Preservation; D048430:Cell Shape; D004553:Electric Conductivity; D004912:Erythrocytes / Q000166:cytology; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D011817:Rabbits; D013997:Time Factors