[Study of biomimetic apatite formation on dentine surface].
The aim of this study was investigation of the opportunity of biomimetic growth of apatite on a dentine surface at various methods of its processing. Artificial blood human plasma--simulated body fluid (SBF)--was used as a source of ions. According to the scanning electronic microscope (SEM) and confocal laser scanning microscope (CLSM) and energy dispersive X-ray analysis (EDX) they have revealed the growth of crystals of calcium--deficient apatite. The structure of the formed apatite layer differed depending on a kind of processing of a dentine surface. After acid etching the globules of the apatite are located chaotically and the part of a dentine surface remains free. The removal of the collagen fibres by NaClO promotes uniform, controllable growth of crystals, forming a monolithic layer. The hypermineralisated areas of a tooth create the best conditions for growth. The transformation hydroxyl apatite in a superficial dentine layer can be caused by increasing of pressure at preparing with diamond bur. The growth of crystals of brushite in the smear layer is possible at a storage in water at 37 degrees capital ES, Cyrillic. The crystals have lamellar form and are well integrated in a dentin surface.
Nikolaenko
S A
SA
Lohbauer
U
U
Zipperle
M
M
Dasch
W
W
Petschelt
A
A
Frankenberger
R
R
D001031:Apatites / Q000032:analysis*; D040761:Biomimetic Materials; D020099:Coated Materials, Biocompatible; D003804:Dentin / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D013997:Time Factors
D001031:Apatites; D020099:Coated Materials, Biocompatible