[Determination of atrazine and its metabolites in human urines using gas chromatography].
Atrazine (ATZ) was widely used to control broadleaf weeds. Numerous animal experiments have proved that atrazine is a suspicious endocrine disruptor. Thus, further development of the ability to estimate low-dose human exposure to atrazine is requested in epidemiologic studies to correlate the toxicological effects associated with the concentrations of ATZ and its metabolites in human body. A method for detecting ATZ and its metabolites (deethylatrazine (DEA), deisopropylatrazine (DIA), deethyldeisopropylatrazine (DEDIA)) in human urines using gas chromatography was established. A urine sample was extracted by ethyl acetate, and purified using a Florisil column. Final concentrated extract was analyzed by a gas chromatograph-electron capture detector. The conditions of this method were optimized. The limits of detection were 0. 002 5 mg/L for DEDIA, 0. 005 mg/L for DEA, DIA and ATZ. The linear ranges were from 0.2 to 8 ng for all analytes. The atrazine concentrations in urine samples of the workers collected from an atrazine plant were determined by this method. The concentration ranges were 0.003 -0.301 mg/L for DEDIA, 0.005 -0.011 mg/L for DEA, 0.006 -0.276 mg/L for DIA, and 0.005 -0.012 mg/L for ATZ.
Xu
Renji
R
Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China. numashijia@hotmail.com
Wei
Fusheng
F
Wang
Yonghua
Y
Hu
Wei
W
Ye
Xinqiang
X
Xu
Guowang
G
D001280:Atrazine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D015394:Molecular Structure; D015203:Reproducibility of Results; D014227:Triazines / Q000737:chemistry / Q000652:urine*
C445502:6-chloro-N-ethyl-1,3-5-triazine-2,4-diamine; D014227:Triazines; C446541:deethylatrazine; D001280:Atrazine