Contents of pentosidine in the tissue of the intervertebral disc as an indicator of the human age.
The study deals with the post-translational modifications of proteins - glycation of the tissue of the intervertebral disc and determination of one of advanced glycation end's products - pentosidine in the relation to the age. Pentosidine was detected in the hydrolysate of the intervertebral discs from persons between the ages of 16 and 95 years. 142 samples were analysed by high performance liquid chromatography, and the detected amounts of pentosidine were processed statistically. The coefficient of correlation of dependence of the amount of pentosidine on the age amounts to r = 0.92. The results of the work testify to the fact that it is possible to use the detection of pentosidine in the tissue of the intervertebral disc for the estimation of the age. Nevertheless subsequent experiments should be done under different conditions post-mortem decomposition.
Pillin
A
A
Institute of Forensic Medicine and Toxicology of the First Faculty of Medicine, Charles University, General Teaching Hospital, Praha, Czech Republic. alexander.pilin@lf1.cuni.cz
Pudil
F
F
Bencko
V
V
Bezdícková
D
D
D000293:Adolescent; D000328:Adult; D000365:Age Determination by Skeleton; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006801:Humans; D007403:Intervertebral Disc / Q000737:chemistry*; D008159:Lumbar Vertebrae; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D008875:Middle Aged
D017127:Glycation End Products, Advanced; D001120:Arginine; C062187:pentosidine; D008239:Lysine