Time course proteomic profile of rat acute myocardial infarction by SELDI-TOF MS analysis.
BACKGROUND
Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF MS) is a powerful tool to detect biomarkers of many diseases. The purpose of this study is to evaluate SELDI-TOF MS as a potential tool for identifying serological biomarkers of acute myocardial infarction (AMI) at an early stage.


METHODS
Serum samples were collected from rats after they had received left anterior descending coronary artery (LAD) ligation at 5, 15, 30, 60, 120, 240 and 360 min, respectively. These samples, along with serum samples from the sham-operated and uninjured control animals, were processed and analyzed with CM-10 ProteinChip and SELDI-TOF MS. Levels of myocardial creatine kinase isoenzyme MB (CKMB) activity and cardiac troponin I (cTnI) concentration were measured with respective commercial kits. Histological changes were examined after HE staining.


RESULTS
Three peaks with m/z 7586 Da, 7564 Da and 9583 Da, which were only found in the operated groups, were termed specific peaks. The peaks with m/z 7586 and 7564 Da had the sensitivity of 97.0% and specificity of 97.0% in the >or=15 min operated groups. Four peaks with m/z 4983, 5140, 8075, and 9423 Da were novel tissue unspecific injury related peaks in response to injury. Four peaks with m/z 4400, 4542, 8400 and 7666 Da exhibited more than 2 fold peak height alterations. In contrast, the significant increase of CKMB activity and cTnI concentration in the serum could not be detected until 4 h after the ligation.


CONCLUSIONS
The specific peaks and unspecific injury related peaks detected by SELDI-TOF MS with CM-10 ProteinChip may be used as the biomarkers for early diagnosis of AMI.
Zhang
Gengqian
G
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, PR China.
Zhou
Bin
B
Zheng
Yanhua
Y
Feng
Kai
K
Rao
Li
L
Zhang
Jianzhong
J
Xin
Junping
J
Zhang
Beilei
B
Zhang
Lin
L
D000818:Animals; D008297:Male; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors