Partial purification of factors for differential transcription of the rrnD promoters for ribosomal RNA synthesis in Streptomyces coelicolor.
The Streptomyces coelicolor A3(2) genome contains six operons (rrnA to F) for ribosomal RNA synthesis. Transcription from rrnD occurs from four promoters (p1 to p4). We found that transcripts from the p1 and p3 promoters were most abundant in vivo in the early exponential phase. However, at later phases of exponential and stationary growth, transcripts from the p1 promoter decreased drastically, with the p3 and p4 transcripts constituting the major forms. Partially purified RNA polymerase supported transcription from the p3 and p4 promoters, whereas pure reconstituted RNA polymerase with core enzyme (E) and the major vegetative sigma factor sigmaHrdB (E.sigmaHrdB) did not. In order to assess any potential requirement for additional factor(s) that allow transcription from the p3 and p4 promoters, we fractionated a partially purified RNA polymerase preparation by denaturing gel filtration chromatography. We found that transcription from the p3 and p4 promoters required factor(s) of about 30-35 kDa in addition to RNAP holoenzyme (E.sigmaHrdB). Therefore, transcription from the p3 and p4 promoters, which contain a consensus -10 region but no -35 for sigmaHrdB recognition, are likely to be regulated by transcription factor(s) that modulate RNA polymerase holoenzyme activity in S. coelicolor.
Hahn
Mi-Young
MY
Genome Research Center for Respiratory Pathogens, Yonsei University College of Medicine, Seoul, Republic of Korea.
Roe
Jung-Hye
JH
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D012335:RNA, Ribosomal; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases