Evaluation of the radioprotective effects of propolis and flavonoids in gamma-irradiated mice: the alkaline comet assay study.
The radioprotective effects of water-soluble derivate of propolis (WSDP) collected in Croatia, and single flavonoids, caffeic acid, chrysin and naringin in the whole-body irradiated CBA mice were investigated. Irradiation was performed using a gamma-ray source ((60)Co), and absorbed doses were 4 and 9 Gy. The efficiency of test components was evaluated when given intraperitoneally (i.p.) at dose of 100 mg kg(-1) for 3 consecutive days before and/or after irradiation. Moreover, possible genotoxic effects of all test components were assessed on non-irradiated animals. The higher efficiency of test components was observed when given preventively. The results suggest that propolis and related flavonoids given to mice before irradiation protected mice from lethal effects of whole-body irradiation and diminish primary DNA damage in their white blood cells as detected by the alkaline comet assay.
Benković
Vesna
V
Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, HR-10000 Zagreb,Croatia. vesna@pmf.biol.hr
Orsolić
Nada
N
Knezević
Anica Horvat
AH
Ramić
Snjezana
S
Dikić
Domagoj
D
Basić
Ivan
I
Kopjar
Nevenka
N
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage; D005419:Flavonoids / Q000494:pharmacology*; D005720:Gamma Rays; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011429:Propolis / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D000975:Antioxidants; D005419:Flavonoids; D011837:Radiation-Protective Agents; D011429:Propolis