Effect of expression of Jagged1 protein in stromal sublayer on the maintenance of hemopoietic precursors in in vitro coculturing system.
In order to verify the relationship between the expression of Notch ligand in NIH 3T3 fibroblast clones and maintenance of hemopoietic activity in vitro, monolayers from three clones were co-cultured with mouse bone marrow mononuclears for 1-4 weeks and the total production of hemopoietic cells and myeloid colony-forming activity in methylcellulose were evaluated. One of the clones exhibited significant hemopoiesis maintaining activity and after 2 weeks of culturing promoted a more than 100-fold increase in the number of colony-forming precursors in comparison with control cells. Hemopoiesis maintenance activity of the second clone was much lower, and that of the third clone virtually did not differ from that of control cells. The results indicate that expression of Notch ligand can radically increase cell strain capacity to maintenance of in vitro hemopoiesis in the co-culturing system.
Rozov
F N
FN
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Belyavskii
A V
AV
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003114:Colony-Forming Units Assay; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D015151:Immunoblotting; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D000072099:Serrate-Jagged Proteins; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014162:Transfection
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C000606274:Jag1 protein, mouse; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D000072099:Serrate-Jagged Proteins