Helicobacter pylori heat shock protein B (HspB) localizes in vivo in the gastric mucosa and MALT lymphoma.
Heat shock protein B (HspB) is one of the dominant proteins recognized by most Helicobacter pylori-infected persons and is being considered as potential candidates for subunit vaccines. In the present study we describe the generation of an antibody against HspB and its use in immunohistochemical assays on gastric biopsies. We have demonstrated that our rabbit polyclonal antibody against HspB did not recognize any protein in lysates from a lung human epithelial cell (H1299) line and did not cross-react with the other members of human heat shock proteins. Secondly, we have observed that in gastric biopsies, HspB immunostaining was present inside the cytoplasm of human epithelial cells with a particular localization in the apical portion of gastric epithelial cells other than in the extracellular spaces among gastric cells of human stomach. Finally, we have demonstrated a cytoplasmic HspB immunostaining in groups of neoplastic cells of MALT lymphoma. In conclusion, our observations suggest a possible involvement of HspB in the pathogenesis of H. pylori-related pathologies such as gastritis, ulcer and gastric cancer.
De Luca
Antonio
A
Department of Medicine and Public Health, Second University of Naples, Naples, Italy. antonio.deluca@unina2.it
De Falco
Maria
M
Manente
Lucrezia
L
Dattilo
Doriana
D
Lucariello
Angela
A
Esposito
Vincenzo
V
Gnarini
Mariarosaria
M
Citro
Gennaro
G
Baldi
Alfonso
A
Tufano
Maria Antonietta
MA
Iaquinto
Gaetano
G
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000378:metabolism; D001706:Biopsy; D002460:Cell Line; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D016481:Helicobacter Infections / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018442:Lymphoma, B-Cell, Marginal Zone / Q000378:metabolism* / Q000473:pathology; D011817:Rabbits
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; C112564:HspB protein, Helicobacter pylori