Disinfection of Bacillus subtilis spore-contaminated surface materials with a sodium hypochlorite and a hydrogen peroxide-based sanitizer.
OBJECTIVE
To evaluate a sodium hypochlorite and hydrogen peroxide solution (Ox-B7) as a potential decontaminant of Bacillus subtilis spore-contaminated surface materials (porous and nonporous).


RESULTS
Test materials were contaminated with B. subtilis spores to a final concentration in the range of 5.7-6.6 log CFU cm(-2). Ox-B7 reduced spore counts by 99.999% (5 log) for both porous and nonporous surfaces within a 5-min contact. Treatment with equivalent concentrations of only sodium hypochlorite reduced spore counts by 99% (2 log) on porous materials and by 99.99% (4 log) on nonporous materials. Hydrogen peroxide treatments reduced spores by less than 90% (<1 log) on both porous and nonporous materials when compared with untreated samples.


CONCLUSIONS
A combination of sodium hypochlorite and hydrogen peroxide (Ox-B7) effectively killed B. subtilis spores on both porous and nonporous surface materials.


CONCLUSIONS
The combination of sodium hypochlorite and hydrogen peroxide can be used as an alternative disinfectant of spore-contaminated surface materials, as it is more effective than when hydrogen peroxide or sodium hypochlorite are used separately.
DeQueiroz
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GA
Audubon Sugar Institute, Louisiana State University Agricultural Center, St. Gabriel, Louisiana 70776, USA. gdequeiroz@agcenter.lsu.edu
Day
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DF
D001412:Bacillus subtilis / Q000187:drug effects*; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D005430:Floors and Floorcoverings; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016062:Porosity; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects*; D014934:Wood / Q000382:microbiology
D004202:Disinfectants; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite