title: Oxidized low-density lipoprotein induces differentiation of RAW264.7 murine macrophage cell line into dendritic-like cells.
abstract: Dendtritic cells (DCs) are potent antigen-presenting cells and have an important role in the pathogenesis of atherosclerosis. Recent data suggests oxidized low-density lipoprotein (oxLDL) promotes the transition of a differentiating monocyte to a mature dendritic cell. In this study, we examined whether oxLDL could induce the differentiation of mature macrophages into DCs. After 48 h treatment with oxLDL, RAW264.7 cells increased in cell size and exhibited dendritic morphology. At the optimal oxLDL dose (10 microg/ml), approximately 74% of RAW264.7 cells differentiated into dendritic-like cells. Flow cytometric analysis detected dendritic cell surface markers (CD83, CD40, CD86, MHC Class II, and CD1d), and their expression increased in a dose- and time-dependent manner. Moreover, oxLDL-treated RAW264.7 cells showed functional changes including reduced endocytic activity, increased allostimulatory activity, and IL-12 production. The findings of the present work demonstrate that RAW264.7 cells, incubated with oxLDL, acquire some dendritic cell features.
authors.affiliation: Department of Cardiology, Ren Ji Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 200127, China.
authors.forename: Ling-Hong
authors.initials: LH
authors.lastname: Shen
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Bin-Yao
authors.initials: BY
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Pu
authors.forename: Liu-Hua
authors.initials: LH
authors.lastname: Hu
authors.forename: Da-Jun
authors.initials: DJ
authors.lastname: Chai
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Jin-Zhang
authors.initials: JZ
authors.lastname: Zeng
authors.forename: Ben
authors.initials: B
authors.lastname: He
mesh_terms: D000818:Animals; D050197:Atherosclerosis; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D016207:Cytokines; D008077:Lipoproteins, LDL; D010100:Oxygen