title: S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells.
abstract: S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.
authors.affiliation: Department of Surgery P.Valdoni, University of Rome La Sapienza, Italy. Fabrizio.Danselmi@uniroma1.it
authors.forename: Fabrizio
authors.initials: F
authors.lastname: D'Anselmi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Cucina
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cavallaro
authors.forename: Mariano
authors.initials: M
authors.lastname: Bizzarri
authors.forename: Rosaria Adele
authors.initials: RA
authors.lastname: Cavallaro
authors.forename: Andrea
authors.initials: A
authors.lastname: Fuso
authors.forename: Antonino
authors.initials: A
authors.lastname: Cavallaro
authors.forename: Sigfrido
authors.initials: S
authors.lastname: Scarpa
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000201:enzymology; D002478:Cells, Cultured; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000201:enzymology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D061988:Proteasome Inhibitors; D051381:Rats; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D025801:Ubiquitin / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D061988:Proteasome Inhibitors; D025801:Ubiquitin; D012436:S-Adenosylmethionine; D046988:Proteasome Endopeptidase Complex