Application of analytical and preparative high-speed counter-current chromatography for the separation of Z-ligustilide from a crude extract of Angelica sinensis.
Z-Ligustilide was separated and purified from the traditional Chinese medicinal plant Angelica sinensis by high-speed counter-current chromatography (HSCCC). Analytical HSCCC was first used for the systematic selection of the two-phase solvent system. Preparative HSCCC separation was performed with a two-phase solvent system composed of petroleum ether (60-90 degrees C)-ethanol-water at an optimum volume ratio of 10:17:10 (v/v). A total of 38 mg Z-ligustilide at 98.8% purity was obtained in one step from 200 mg crude extract as determined by HPLC analysis. The structure of the target compound was identified by electron impact ionisation mass spectrometry.
Wang
Xiao
X
Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, People's Republic of China.
Shi
Xingang
X
Li
Fuwei
F
Liu
Jianhua
J
Cheng
Chuange
C
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry; D029971:Angelica sinensis / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*
D010936:Plant Extracts; C027820:ligustilide; D015107:4-Butyrolactone