Adr1 and Cat8 mediate coactivator recruitment and chromatin remodeling at glucose-regulated genes.
BACKGROUND
Adr1 and Cat8 co-regulate numerous glucose-repressed genes in S. cerevisiae, presenting a unique opportunity to explore their individual roles in coactivator recruitment, chromatin remodeling, and transcription.


RESULTS
We determined the individual contributions of Cat8 and Adr1 on the expression of a cohort of glucose-repressed genes and found three broad categories: genes that need both activators for full derepression, genes that rely mostly on Cat8 and genes that require only Adr1. Through combined expression and recruitment data, along with analysis of chromatin remodeling at two of these genes, ADH2 and FBP1, we clarified how these activators achieve this wide range of co-regulation. We find that Adr1 and Cat8 are not intrinsically different in their abilities to recruit coactivators but rather, promoter context appears to dictate which activator is responsible for recruitment to specific genes. These promoter-specific contributions are also apparent in the chromatin remodeling that accompanies derepression: ADH2 requires both Adr1 and Cat8, whereas, at FBP1, significant remodeling occurs with Cat8 alone. Although over-expression of Adr1 can compensate for loss of Cat8 at many genes in terms of both activation and chromatin remodeling, this over-expression cannot complement all of the cat8Delta phenotypes.


CONCLUSIONS
Thus, at many of the glucose-repressed genes, Cat8 and Adr1 appear to have interchangeable roles and promoter architecture may dictate the roles of these activators.
Biddick
Rhiannon K
RK
Department of Biochemistry, University of Washington, Seattle, Washington, United States of America.
Law
G Lynn
GL
Young
Elton T
ET
D002843:Chromatin / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012319:RNA Polymerase II / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D015534:Trans-Activators / Q000502:physiology*; D035581:Transcription Factor TFIIB / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*
C074682:ADR1 protein, S cerevisiae; C092600:CAT8 protein, S cerevisiae; D002843:Chromatin; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D012319:RNA Polymerase II; D005947:Glucose