Inactivation of mitochondrial respiratory chain complex I leads mitochondrial nitric oxide synthase to become pro-oxidative.
We recently demonstrated that mitochondrial nitric oxide synthase (mtNOS) functionally couples with mitochondrial respiratory chain complex I to produce nitric oxide [M.S. Parihar, R.R. Nazarewicz, E. Kincaid, U. Bringold, P. Ghafourifar, Association of mitochondrial nitric oxide synthase activity with respiratory chain complex I, Biochem. Biophys. Res. Commun. 366 (2008) 23-28]. The present report shows that inactivation of complex I leads mtNOS to become pro-oxidative. Our findings suggest a crucial role for mtNOS in oxidative stress caused by mitochondrial complex I inactivation.
Parihar
Mordhwaj S
MS
Department of Surgery, Davis Heart and Lung Research Institute, and Institute of Mitochondrial Biology, The Ohio State University, Columbus, OH 43210, USA.
Parihar
Arti
A
Villamena
Frederick A
FA
Vaccaro
Patrick S
PS
Ghafourifar
Pedram
P
D000818:Animals; D019069:Cell Respiration / Q000502:physiology; D002478:Cells, Cultured; D042967:Electron Transport Complex I / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
D017382:Reactive Oxygen Species; D013481:Superoxides; D019001:Nitric Oxide Synthase; D042967:Electron Transport Complex I; D010100:Oxygen