Mycobacterial T cell responses in HIV-infected patients with advanced immunosuppression.
Antimycobacterial T cell reactivity at different stages of HIV infection was investigated. Subjects were screened with purified protein derivative (PPD), early secreted antigenic target (ESAT)-6, and culture filtrate protein (CFP)-10 antigens for interferon (IFN)-gamma-producing effector T cell responses by direct ex vivo enzyme-linked immunospot (ELISpot) assay. The proportion of responders to PPD tuberculin decreased with a reduction in CD4 T cell count, whereas the proportion of responder to ESAT-6 and CFP-10 did not. The main sources of IFN-gamma secretion were CD4 cells, and the relative responses to ESAT-6 and CFP-10 significantly increased in HIV-infected patients with decreasing CD4 cell count. This may reflect early signs of reactivation, reinfection, or a restricted, inefficient immune response to Mycobacterium tuberculosis.
Hammond
Abdulrahman S
AS
Bacterial and Viral Diseases Programme, Medical Research Council Laboratories, Fajara, Banjul, The Gambia, West Africa. ahammond@mrc.gm
McConkey
Samuel J
SJ
Hill
Philip C
PC
Crozier
Sarah
S
Klein
Michèl R
MR
Adegbola
Richard A
RA
Rowland-Jones
Sarah
S
Brookes
Roger H
RH
Whittle
Hilton
H
Jaye
Assan
A
D017088:AIDS-Related Opportunistic Infections / Q000276:immunology / Q000382:microbiology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015658:HIV Infections / Q000150:complications* / Q000276:immunology* / Q000821:virology; D015497:HIV-1; D015498:HIV-2; D006801:Humans; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000150:complications / Q000276:immunology / Q000382:microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D014373:Tuberculin; D007371:Interferon-gamma