[Revisiting HIV-1 assembly].
During the late stage of virus replication, incorporation of the envelope glycoproteins (Env) by Gag cores takes place together with the proteolytic maturation of Gag and Gag-Pol precursors. Assembly is initially driven by Gag oligomerisation, which requires two platorms. The first one is formed by specific membrane subdomains with which Gag molecules interact via the N-terminal MA domain, and the second by the viral genomic RNA undergoing specific interactions with the NC domain of Gag. To complete viral budding, the Gag "late domain" subsequently associates with members of the ESCRT complexes involved in the budding of vesicles in late endosomes (LE). While the cellular trafficking of the viral components is still poorly understood, there is an ongoing debate on the site of HIV-1 assembly, because this process might take place either at the plasma membrane or in intracellular compartments such as the LE, depending on the virus/cell system studied. This site may depend on the interplay of multiple overlapping trafficking signals bear by Gag and Env. Our recent results indicate that it may rely on the chronic or acute nature of the viral infection more than on the cell type. In chronically infected cells, virions probably assemble and accumulate in intracellular compartments hidden from the immune system. Release of virions in the form of bursts would be triggered during cell-cell interactions, through a specialized structure called the virological synapse.
Corbin
Antoine
A
Laborétro Unité de virologie humaine, INSERM U758, IFR128, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, Lyon, France.
Grigorov
Boyan
B
Roingeard
Philippe
P
Darlix
Jean-Luc
JL
Muriaux
Delphine
D
D000163:Acquired Immunodeficiency Syndrome / Q000473:pathology; D002908:Chronic Disease; D015683:Gene Products, gag / Q000502:physiology; D015497:HIV-1 / Q000502:physiology* / Q000648:ultrastructure*; D006801:Humans; D014759:Viral Envelope Proteins / Q000502:physiology
D015683:Gene Products, gag; D014759:Viral Envelope Proteins