Deletion of the SH gene from avian metapneumovirus has a greater impact on virus production and immunogenicity in turkeys than deletion of the G gene or M2-2 open reading frame.
Subgroup A avian metapneumoviruses lacking either the SH or G gene or the M2-2 open reading frame were generated by using a reverse-genetics approach. The growth properties of these viruses were studied in vitro and in vivo in their natural host. Deletion of the SH gene alone resulted in the generation of a syncytial-plaque phenotype and this was reversed by the introduction of the SH gene from a subgroup B, but not a subgroup C, virus. Infected turkeys were assessed for antibody production and the presence of viral genomic RNA in tracheal swabs. The virus with a deleted SH gene also showed the greatest impairment of replication both in cell culture and in infected turkeys. This contrasts with the situation with other pneumoviruses in culture and in model animals, where deletion of the SH gene results in little effect upon viral yield and a good antibody response. Replication of the G- and M2-2-deleted viruses was impaired more severely in turkeys than in cell culture, with only some animals showing evidence of virus growth and antibody production. There was no correlation between virus replication and antibody response, suggesting that replication sites other than the trachea may be important for induction of antibody responses.
Ling
Roger
R
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Sinkovic
Sabrina
S
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
Toquin
Didier
D
French Agency for Food Safety (AFSSA), OIE Reference Laboratory for Turkey Rhinotracheitis, Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), BP 53, 22440 Ploufragan, France.
Guionie
Olivier
O
French Agency for Food Safety (AFSSA), OIE Reference Laboratory for Turkey Rhinotracheitis, Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), BP 53, 22440 Ploufragan, France.
Eterradossi
Nicolas
N
French Agency for Food Safety (AFSSA), OIE Reference Laboratory for Turkey Rhinotracheitis, Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), BP 53, 22440 Ploufragan, France.
Easton
Andrew J
AJ
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
D000818:Animals; D000914:Antibodies, Viral; D002460:Cell Line; D017353:Gene Deletion; D005814:Genes, Viral / Q000235:genetics; D029121:Metapneumovirus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D016366:Open Reading Frames; D018184:Paramyxoviridae Infections / Q000276:immunology* / Q000821:virology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G