Purification and antitumour activity of a lipopeptide biosurfactant produced by Bacillus natto TK-1.
An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.
Cao
Xiao-Hong
XH
School of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China. caoxh@tust.edu.cn
Liao
Zhen-Yu
ZY
Wang
Chun-Ling
CL
Cai
Ping
P
Yang
Wen-Yan
WY
Lu
Mei-Fang
MF
Huang
Guo-Wei
GW
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001407:Bacillus / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004435:Eating; D004789:Enzyme Activation / Q000187:drug effects; D005453:Fluorescence; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D055666:Lipopeptides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009821:Oils / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
D000970:Antineoplastic Agents; D055666:Lipopeptides; D009821:Oils; D013501:Surface-Active Agents; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3