title: The effect of single and repeated UVB radiation on rabbit lens.
abstract: BACKGROUND
  In our previous investigations, a significant cumulative effect of ultraviolet radiation (UVR) on the corneal and aqueous humour metabolic profiles was revealed. The purpose of the present study was to monitor the alterations in the rabbit lenses under the same experimental design and thereby supplement and complete prior findings.
  
  
  METHODS
  Albino rabbit eyes were exposed to single (312 nm, 3.12 J/cm2) or repeated (312 nm, 3 x 1.04 J/cm2) UVB irradiations of the same overall doses. Lenticular samples were analysed by high resolution magic angle spinning proton nuclear magnetic resonance (HR-MAS 1H NMR) spectroscopy. Special grouping patterns between the UVB-irradiated and untreated control samples were evaluated using principal component analysis (PCA). Percentage alterations in the lenticular metabolite concentrations from UVR-B exposed rabbits were calculated relative to the levels in the control group.
  
  
  RESULTS
  UVB irradiation of the albino rabbit lenses resulted in a significant decrease in the concentrations of antioxidants (glutathione), osmolytes (taurine, myoinositol) and amino acids (alanine), and a concomitant elevation in the contents of a sugar-related compound, sorbitol. Repeated UVR-B exposure of the rabbit eye had a stronger effect on the lenticular metabolic profile than a single irradiation of the same overall dose.
  
  
  CONCLUSIONS
  This study reveals the cumulative effect of repeated UVB irradiations, and shows that even a 48-hour interval between subsequent UVR-B exposures is not sufficient for the healing processes to restore lenticular integrity.
authors.affiliation: Department of Neuroscience, Faculty of Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway. miroslav.fris@ntnu.no
authors.forename: Miroslav
authors.initials: M
authors.lastname: Fris
authors.forename: Jitka
authors.initials: J
authors.lastname: Cejková
authors.forename: Anna
authors.initials: A
authors.lastname: Midelfart
mesh_terms: D000409:Alanine / Q000378:metabolism; D000818:Animals; D002386:Cataract / Q000209:etiology* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011817:Rabbits; D011833:Radiation Injuries, Experimental / Q000209:etiology* / Q000378:metabolism; D013654:Taurine / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D013654:Taurine; D007294:Inositol; D005978:Glutathione; D000409:Alanine