Functional and bioinformatics analyses reveal conservation of cis-regulatory elements between sciaridae and drosophilidae.
The sciarid DNA puff C4 BhC4-1 gene is amplified and transcribed in salivary glands at the end of the larval stage. In transgenic Drosophila, the BhC4-1 promoter drives transcription in prepupal salivary glands and in the ring gland of late embryos. A bioinformatics analysis has identified 162 sequences similar to distinct regions of the BhC4-1 proximal promoter, which are predominantly located either in 5' or 3' regions or introns in the Drosophila melanogaster genome. A significant number of the identified sequences are found in the regulatory regions of Drosophila genes that are expressed in the salivary gland. Functional assays in Drosophila reveal that the BhC4-1 proximal promoter contains both a 129 bp (-186/-58) salivary gland enhancer and a 67 bp (-253/-187) ring gland enhancer that drive tissue specific patterns of developmentally regulated gene expression, irrespective of their orientation.
Lecci
Matheus S
MS
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas (DACTB), Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP), Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil.
Malta
Tathiane M
TM
Flausino
Vinícius T
VT
Gitaí
Daniel L G
DL
Ruiz
Jerônimo C
JC
Monesi
Nadia
N
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D019295:Computational Biology / Q000379:methods*; D017124:Conserved Sequence; D004175:Diptera / Q000235:genetics*; D018428:Drosophilidae / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D017344:Genes, Insect; D005809:Genes, Regulator; D016678:Genome; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D013045:Species Specificity