Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.
LIM mineralization protein 1 (LMP-1) is an essential positive regulator of osteoblast differentiation and maturation and bone formation. However, the expression and distribution of LMP-1 in human teeth are largely unknown. The aim of this study was to detect the expression of LMP-1 in normal healthy human teeth and human teeth with various pathologic conditions. LMP-1 expression was determined by immunohistochemistry in all of the samples including 12 healthy teeth, 10 teeth with caries, 12 teeth with pulpitis, and 4 teeth with pulp calcification. We found that LMP-1 was expressed primarily in predentin, odontoblasts, and endothelial cells of the blood vessels of healthy teeth. In addition, LMP-1 expression was also found in unmineralized reparative dentin, odontoblast-like cells, and pulp fibroblasts in teeth with caries and pulpitis. Furthermore, we found LMP-1 expression on the surface of the pulp stone or in the residual predentin of teeth with pulp calcification. Our data suggest that LMP-1 plays a role in odontoblast differentiation and dentin matrix mineralization of human teeth with normal and pathologic conditions.
Wang
Xiaoying
X
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Zhang
Qi
Q
Chen
Zhi
Z
Zhang
Lu
L
D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D003598:Cytoskeletal Proteins; D003731:Dental Caries / Q000473:pathology*; D003782:Dental Pulp / Q000473:pathology*; D003784:Dental Pulp Calcification / Q000473:pathology; D003788:Dental Pulp Diseases / Q000473:pathology*; D003804:Dentin / Q000473:pathology*; D003809:Dentin, Secondary / Q000473:pathology; D003810:Dentinogenesis / Q000502:physiology; D042783:Endothelial Cells / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis*; D060588:LIM Domain Proteins; D008297:Male; D009804:Odontoblasts / Q000473:pathology; D011671:Pulpitis / Q000473:pathology; D016335:Zinc Fingers
D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D060588:LIM Domain Proteins; C484380:PDLIM7 protein, human