A direct protein-protein interaction is involved in the suppression of beta-catenin transcription by retinoid X receptor alpha in colorectal cancer cells.
Using both mammalian two-hybrid assay in vivo and immunoprecipitation in vitro we found that retinoid X receptor alpha (RXR alpha) directly interacted with beta-catenin and suppressed beta-catenin transcription activity and protein expression in colorectal cancer cells. But, reduction of RXR alpha by small interfering RNA upregulated beta-catenin transcriptional activity and protein expression. However, gain- or loss-expression of beta-catenin did not affect RXR alpha. Therefore, our data provided the first evidence tht RXR alpha directly interacted with beta-catenin and regulated beta-catenin transcription, which provides important information for developing novel strategies in colorectal cancer prevention by targeting RXR alpha-beta-catenin signaling.
Han
Anjia
A
Department of Pathology, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
Tong
Chang
C
Hu
Dong
D
Bi
Xiuli
X
Yang
Wancai
W
D000199:Actins / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D006801:Humans; D012334:RNA, Neoplasm / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D047490:Retinoid X Receptor alpha / Q000502:physiology*; D014158:Transcription, Genetic; D051176:beta Catenin / Q000235:genetics / Q000502:physiology*
D000199:Actins; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D047490:Retinoid X Receptor alpha; D051176:beta Catenin