title: Reciprocal subtraction differential RNA display (RSDD): an efficient technology for cloning differentially expressed genes.
abstract: Identification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological and pathological processes. Subtraction hybridization and differential RNA display (DDRT-PCR) are two methods that are widely and successfully employed to clone differentially expressed genes. Unfortunately, both methods have inherent problems and limitations requiring improvements in the technique. A combination of these two methods termed reciprocal subtraction differential RNA display is described here that considerably reduces the complexity of DDRT-PCR and facilitates the rapid and efficient identification and cloning of both abundant and rare differentially expressed genes.
authors.affiliation: Department of Urology, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY, USA.
authors.forename: Devanand
authors.initials: D
authors.lastname: Sarkar
authors.forename: Dong-chul
authors.initials: DC
authors.lastname: Kang
authors.forename: Paul B
authors.initials: PB
authors.lastname: Fisher
mesh_terms: D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods*; D004277:DNA, Single-Stranded; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012313:RNA
chemical_list: D004277:DNA, Single-Stranded; D012313:RNA