Cell effects of xenon in vitro under hypothermal conditions.
The effects of Xe on cell viability and redox balance in the culture of Wistar rat thymocytes were studied in vitro during 24-h storage under hypothermic conditions. The results indicate that after bubbling of cell suspensions (5 x 10(6) cell/ml, 4 ml medium), the weight of Xe in flasks was 15-43 mg, whereas in cell-free medium no weight increment due to gas accumulation in the system was detected. The content of Xe in cell suspension slightly decreased over 24-h culturing at ambient temperature (by 10% of initial level). Xenon significantly improved cell survival during thermal exposure of all modes. The maximum cytoprotective effect of Xe was observed under rigorous thermal conditions associated with significant cell death without chemical protectors (3 degrees C, -35 degrees C). The effect of Xe was less pronounced at mild temperatures (23-37 degrees C) or in the presence of chemical protectors (-35 degrees C with dimethylsulfoxide). The mechanisms of the effect of the inert gas are determined by its antioxidant or prooxidant action. The capacity of Xe to improve cell survival under hypothermic conditions can be used for the development of new methods for transportation and storage of cell material.
Khlusov
I A
IA
Siberian State Medical University. khl@ultranet.tomsk.ru
Zhukov
P G
PG
Sukhikh
G T
GT
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003080:Cold Temperature; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism; D014978:Xenon / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*
D014978:Xenon