Spectroscopic and functional characterization of human beta-synuclein.
To elucidate potential biological roles of human beta-synuclein, we studied its conformational changes under various conditions. The structural and functional properties of beta-synuclein were characterized using biochemical and bio-physical methods including: a functional assay, mass spectrometry, size exclusion chromatography, circular dichroism (CD), and fluorescence spectroscopy. The results showed beta-synuclein has a high proportion of random coil in solution.
Lee
Juhyung
J
Department of Molecular Science and Technology, Graduate School of Interdisciplinary Program, Ajou University, Suwon, South Korea.
Hwang
Heejin
H
Kim
T Doohun
TD
D002942:Circular Dichroism; D006801:Humans; D013058:Mass Spectrometry; D008670:Metals / Q000494:pharmacology; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D051846:beta-Synuclein / Q000737:chemistry* / Q000378:metabolism*
D008670:Metals; D018832:Molecular Chaperones; D051846:beta-Synuclein