title: [FeFe]-hydrogenase-catalyzed H2 production in a photoelectrochemical biofuel cell.
abstract: The Clostridium acetobutylicum [FeFe]-hydrogenase HydA has been investigated as a hydrogen production catalyst in a photoelectrochemical biofuel cell. Hydrogenase was adsorbed to pyrolytic graphite edge and carbon felt electrodes. Cyclic voltammograms of the immobilized hydrogenase films reveal cathodic proton reduction and anodic hydrogen oxidation, with a catalytic bias toward hydrogen evolution. When corrected for the electrochemically active surface area, the cathodic current densities are similar for both carbon electrodes, and approximately 40% of those obtained with a platinum electrode. The high surface area carbon felt/hydrogenase electrode was subsequently used as the cathode in a photoelectrochemical biofuel cell. Under illumination, this device is able to oxidize a biofuel substrate and reduce protons to hydrogen. Similar photocurrents and hydrogen production rates were observed in the photoelectrochemical biofuel cell using either hydrogenase or platinum cathodes.
authors.affiliation: Center for Bioenergy and Photosynthesis and Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Hambourger
authors.forename: Miguel
authors.initials: M
authors.lastname: Gervaldo
authors.forename: Drazenka
authors.initials: D
authors.lastname: Svedruzic
authors.forename: Paul W
authors.initials: PW
authors.lastname: King
authors.forename: Devens
authors.initials: D
authors.lastname: Gust
authors.forename: Maria
authors.initials: M
authors.lastname: Ghirardi
authors.forename: Ana L
authors.initials: AL
authors.lastname: Moore
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Moore
mesh_terms: D001674:Bioelectric Energy Sources; D002384:Catalysis; D046969:Clostridium acetobutylicum / Q000201:enzymology; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D006864:Hydrogenase / Q000737:chemistry*; D007506:Iron-Sulfur Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D008958:Models, Molecular; D015394:Molecular Structure; D010777:Photochemistry; D010984:Platinum / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D012680:Sensitivity and Specificity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D007506:Iron-Sulfur Proteins; D008567:Membranes, Artificial; D011166:Porphyrins; C009495:titanium dioxide; D010984:Platinum; D006859:Hydrogen; D014025:Titanium; C118830:iron hydrogenase; D006864:Hydrogenase