title: Helper B cells promote cytotoxic T cell survival and proliferation independently of antigen presentation through CD27/CD70 interactions.
abstract: CD8-expressing cytotoxic T cell (CTL) interactions with APCs and helper T cells determine their function and ability to survive. In this study, we describe a novel interaction independent of Ag presentation between activated CTLs and bystander CD19-expressing B lymphocytes. Ag-stimulated CTLs serially engage autologous B lymphocytes through CD27/CD70 contact that promotes their survival and proliferation. Moreover, these interactions induce the release of proinflammatory cytokines that follows two general patterns: 1) an epitope-dependent enhancement of cytokine release, and 2) a previously undiscovered coordinate release of cytokines independent of epitope exposure. The latter includes chemoattractants targeting activated T cells. As a result, activated T cells are attracted to B cells, which exert a "helper" role in lymphatic organs or in areas of inflammation. This observation provides a mechanistic explanation to previously reported experimental observations suggesting that B cells are required for T cell priming in vivo.
authors.affiliation: Infectious Disease and Immunogenetics Section, Department of Transfusion Medicine, Clinical Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Deola
authors.forename: Monica C
authors.initials: MC
authors.lastname: Panelli
authors.forename: Dragan
authors.initials: D
authors.lastname: Maric
authors.forename: Silvia
authors.initials: S
authors.lastname: Selleri
authors.forename: Natalia I
authors.initials: NI
authors.lastname: Dmitrieva
authors.forename: Ching Y
authors.initials: CY
authors.lastname: Voss
authors.forename: Harvey
authors.initials: H
authors.lastname: Klein
authors.forename: David
authors.initials: D
authors.lastname: Stroncek
authors.forename: Ena
authors.initials: E
authors.lastname: Wang
authors.forename: Francesco M
authors.initials: FM
authors.lastname: Marincola
mesh_terms: D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology*; D053282:CD27 Ligand / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D005452:Fluoresceins / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D013388:Succinimides / Q000032:analysis; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism*
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D053282:CD27 Ligand; D016207:Cytokines; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7