Chemosensitization effects of simultaneous suppression of MDR1 and XIAP in multidrug resistant glioma cells.
We studied the mechanisms involved in the drug resistance of C6/ADR and compared the reversal effects of XIAP and MDR1 ASO used in combination and separately. Reverse transcription-PCR and Western blot were applied to detect the mRNA and protein levels, respectively. The results showed that XIAP and MDR1 ASO used separately could down-regulate the levels of XIAP and MDR1 in C6/ADR cells, respectively. XIAP and MDR1 ASO used in combination did not enhance the inhibition expression of XIAP and MDR1 of C6/ADR cells. The apoptosis of co-transfection group was significantly higher than XIAP ASO (P < 0.05). When used in combination the sensitivity of C6/ADR cells to ADR was increased significantly compared with XIAP or MDR1 ASO used separately (P < 0.05). The results indicated that both XIAP and MDR1 were involved in the drug resistance of C6/ADR cells. The sensitivity to ADR could be enhanced significantly when XIAP and MDR1 ASO used in combination.
Zhang
Zhiqing
Z
Department of Neurology, The Central Hospital, 826 South-West-Road, Dalian, 116033, P.R. China. zhangzhiqing1972@yahoo.com.cn
Wang
Xiaofang
X
Wang
Suping
S
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D005434:Flow Cytometry; D005910:Glioma / Q000378:metabolism*; D016376:Oligonucleotides, Antisense; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000378:metabolism*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000903:Antibiotics, Antineoplastic; D016376:Oligonucleotides, Antisense; D051636:X-Linked Inhibitor of Apoptosis Protein; D004317:Doxorubicin