Cdk9/Cyclin T1 complex: a key player during the activation/differentiation process of normal lymphoid B cells.
Cdk9/Cyclin T1 complex is very important in controlling specific differentiative pathways of several cell types. Limited data are available regarding the expression of Cdk9/Cyclin T1 in hematopoietic and lymphoid tissues. Cdk9/Cyclin T1 expression seems to be related to particular stages of lymphoid differentiation/activation. In this study, we observed that the expression level of Cdk9/Cyclin T1 in vivo increases in memory B cells compared to naïve B cells, and in activated B cells, compared to non-activated ones. The expression level of the Cdk9/Cyclin T1 complex does not increase in cells induced to differentiate in vitro. In addition, we showed that Cdk9 interacts with E12 and E47, specifically activated during Germinal Center (GC) reaction. Taken together this data suggests an active role for the Cdk9/Cyclin T1 complex during lymphoid differentiation through germinal center reaction.
De Falco
Giulia
G
Department of Human Pathology and Oncology, University of Siena, Siena, Italy.
Leucci
Eleonora
E
Onnis
Anna
A
Bellan
Cristiana
C
Tigli
Chiara
C
Wirths
Stefan
S
Cerino
Giovanna
G
Cocco
Mario
M
Crupi
Domenica
D
De Luca
Antonio
A
Lanzavecchia
Antonio
A
Tosi
Piero
P
Leoncini
Lorenzo
L
Giordano
Antonio
A
D001402:B-Lymphocytes / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival; D056906:Cyclin T; D042863:Cyclin-Dependent Kinase 9 / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D018858:Germinal Center / Q000201:enzymology; D006801:Humans; D019169:Jurkat Cells; D008198:Lymph Nodes / Q000201:enzymology; D008213:Lymphocyte Activation / Q000276:immunology*; D018613:Microscopy, Confocal; D011485:Protein Binding; D021381:Protein Transport; D051820:TCF Transcription Factors / Q000378:metabolism; D058125:Transcription Factor 7-Like 1 Protein
C503684:CCNT1 protein, human; D056906:Cyclin T; D016213:Cyclins; D051820:TCF Transcription Factors; C544912:TCF7L1 protein, human; D058125:Transcription Factor 7-Like 1 Protein; D042863:Cyclin-Dependent Kinase 9