CRT1, an Arabidopsis ATPase that interacts with diverse resistance proteins and modulates disease resistance to turnip crinkle virus.
Plant immunity frequently involves the recognition of pathogen-encoded avirulence (avr) factors by their corresponding plant resistance (R) proteins. This triggers the hypersensitive response (HR) where necrotic lesions formed at the site(s) of infection help restrict pathogen spread. HRT is an Arabidopsis R protein required for resistance to turnip crinkle virus (TCV). In a genetic screen for mutants compromised in the recognition of TCV's avr factor, we identified crt1 (compromised recognition of TCV), a mutant that prematurely terminates an ATPase protein. Following TCV infection, crt1 developed a spreading HR and failed to control viral replication and spread. crt1 also suppressed HR-like cell death induced by ssi4, a constitutively active R protein, and by Pseudomonas syringae carrying avrRpt2. Furthermore, CRT1 interacts with HRT, SSI4, and two other R proteins, RPS2 and Rx. These data identify CRT1 as an important mediator of defense signaling triggered by distinct classes of R proteins.
Kang
Hong-Gu
HG
Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.
Kuhl
Joseph C
JC
Kachroo
Pradeep
P
Klessig
Daniel F
DF
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000382:microbiology / Q000821:virology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D019184:Carmovirus / Q000472:pathogenicity*; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000472:pathogenicity; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000821:virology
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C491954:HRT protein, Arabidopsis; D012097:Repressor Proteins; C581565:CRT1 protein, Arabidopsis; D004706:Endodeoxyribonucleases; D000251:Adenosine Triphosphatases