Expression of langerin/CD207 reveals dendritic cell heterogeneity between inbred mouse strains.
Langerin/CD207 is expressed by a subset of dendritic cells (DC), the epithelial Langerhans cells. However, langerin is also detected among lymphoid tissue DC. Here, we describe striking differences in langerin-expressing cells between inbred mouse strains. While langerin+ cells are observed in comparable numbers and with comparable phenotypes in the epidermis, two distinct DC subsets bear langerin in peripheral, skin-draining lymph nodes of BALB/c mice (CD11c(high) CD8alpha(high) and CD11c(low) CD8alpha(low)), whereas only the latter subset is present in C57BL/6 mice. The CD11c(high) subset is detected in mesenteric lymph nodes and spleen of BALB/c mice, but is virtually absent from C57BL/6 mice. Similar differences are observed in other mouse strains. CD11c(low) langerin+ cells represent skin-derived Langerhans cells, as demonstrated by their high expression of DEC-205/CD205, maturation markers, and recruitment to skin-draining lymph nodes upon imiquimod-induced inflammation. It will be of interest to determine the role of lymphoid tissue-resident compared to skin-derived langerin+ DC.
Flacher
Vincent
V
Department of Dermatology, Innsbruck Medical University, Innsbruck, Austria. vincent.flacher@i-med.ac.at
Douillard
Patrice
P
Aït-Yahia
Smina
S
Stoitzner
Patrizia
P
Clair-Moninot
Valérie
V
Romani
Nikolaus
N
Saeland
Sem
S
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology*; D004817:Epidermis / Q000276:immunology; D016130:Immunophenotyping; D037181:Lectins, C-Type / Q000378:metabolism*; D008198:Lymph Nodes / Q000276:immunology; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface / Q000378:metabolism; D013045:Species Specificity; D013154:Spleen / Q000276:immunology
D015703:Antigens, CD; D000954:Antigens, Surface; C498497:Cd207 protein, mouse; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface