title: Detection of a gamma-carboxy-glutamate as novel post-translational modification of human transthyretin.
abstract: During analysis of the proteome in the cerebrospinal fluid (CSF) of the Caucasian form of moyamoya disease (MMD), a novel post-translational modification of human transthyretin was observed. Two-dimensional electrophoresis and subsequent peptide sequencing with ESI-MS/MS were performed to discover the gamma-carboxylation of the Glu-42 (Gla-42).
authors.affiliation: European Molecular Biology Laboratory, Proteomic Core Facility, Meyerhofstr. 1. 69117 Heidelberg, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rüggeberg
authors.forename: Peter
authors.initials: P
authors.lastname: Horn
authors.forename: Xinping
authors.initials: X
authors.lastname: Li
authors.forename: Peter
authors.initials: P
authors.lastname: Vajkoczy
authors.forename: Thomas
authors.initials: T
authors.lastname: Franz
mesh_terms: D015055:1-Carboxyglutamic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D000328:Adult; D001665:Binding Sites; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009072:Moyamoya Disease / Q000134:cerebrospinal fluid / Q000378:metabolism*; D011228:Prealbumin / Q000134:cerebrospinal fluid / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D011228:Prealbumin; D020543:Proteome; D015055:1-Carboxyglutamic Acid