title: Scaffold and growth factor selection in temporomandibular joint disc engineering.
abstract: Temporomandibular joint disc tissue-engineering studies commonly fail to produce significant matrix before construct contraction. We hypothesized that poly-L-lactic acid (PLLA) non-woven meshes would limit contraction, allow for comprehensive mechanical evaluation, and maintain viability relative to polyglycolic acid (PGA) non-woven mesh controls. Additionally, we proposed that growth factor stimulation, while limiting contraction, would increase construct properties relative to previous reports. After 4 wks, cell proliferation and matrix deposition were similar between the two meshes, but PGA constructs had contracted significantly. Furthermore, only PLLA constructs could be tested in tension and compression. Additional PLLA constructs were formed, then treated with insulin-like growth factor-1 (10 ng/mL), transforming growth factor-beta 1 (5 ng/mL), or transforming growth factor-beta 3 (5 ng/mL). Transforming growth factor-beta 1 yielded the most cells, collagen, and glycosaminoglycans at 6 wks; these constructs also demonstrated improved mechanics. Analysis of these data demonstrated significant temporomandibular joint disc-engineering potential for PLLA and transforming growth factor-beta 1.
authors.affiliation: Department of Bioengineering, Rice University, MS-142, PO Box 1892, Houston, TX 77251-1892, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Allen
authors.forename: K A
authors.initials: KA
authors.lastname: Athanasiou
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D049109:Cell Proliferation; D003094:Collagen / Q000187:drug effects; D004548:Elasticity; D006025:Glycosaminoglycans / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000627:therapeutic use*; D019344:Lactic Acid / Q000737:chemistry; D009924:Organ Culture Techniques; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013526:Surgical Mesh; D013552:Swine; D019224:Temporomandibular Joint Disc / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology; D014783:Viscosity
chemical_list: D006025:Glycosaminoglycans; D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; D011108:Polymers; D053773:Transforming Growth Factor beta1; D053782:Transforming Growth Factor beta3; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D007334:Insulin-Like Growth Factor I; D003094:Collagen