title: Neuroaxonal ion dyshomeostasis of the normal-appearing corpus callosum in experimental autoimmune encephalomyelitis.
abstract: Atrophy of the corpus callosum (CC) is a well-documented observation in clinically definite multiple sclerosis (MS) patients. One recent hypothesis for the neurodegeneration that occurs in MS is that ion dyshomeostasis leads to neuroaxonal damage. To examine whether ion dyshomeostasis occurs in the CC during MS onset, experimental autoimmune encephalomyelitis (EAE) was utilized as an animal MS model to induce autoimmunity-mediated responses. To date, in vivo investigations of neuronal ion homeostasis has not been feasible using traditional neuroscience techniques. Therefore, the current study employed an emerging MRI method, called Mn2+-enhanced MRI (MEMRI). Mn2+ dynamics is closely associated with important neuronal activity events, and is also considered to be a Ca2+ surrogate. Furthermore, when injected intracranially, Mn2+ can be used as a multisynaptic tracer. These features enable MEMRI to detect neuronal ion homeostasis within a multisynaptic circuit that is connected to the injection site. Mn2+ was injected into the visual cortex to trace the CC, and T1-weighted imaging was utilized to observe temporal changes in Mn2+-induced signals in the traced pathways. The results showed that neuroaxonal functional changes associated with ion dyshomeostasis occurred in the CC during an acute EAE attack. In addition, the pathway appeared normal, although EAE-induced immune-cell infiltration was visible around the CC. The findings suggest that ion dyshomeostasis is a major neuronal aberration underlying the deterioration of normal-appearing brain tissues in MS, supporting its involvement in neuroaxonal functioning in MS.
authors.affiliation: Functional and Micro-Magnetic Resonance Imaging Center, Institute of Biomedical Sciences, Academia Sinica, Taipei, 11529, Taiwan, ROC.
authors.forename: Chiao-Chi V
authors.initials: CC
authors.lastname: Chen
authors.forename: Anil
authors.initials: A
authors.lastname: Zechariah
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Hsu
authors.forename: Hsiao-Wen
authors.initials: HW
authors.lastname: Chen
authors.forename: Li-Chuan
authors.initials: LC
authors.lastname: Yang
authors.forename: Chen
authors.initials: C
authors.lastname: Chang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001931:Brain Mapping; D003337:Corpus Callosum / Q000473:pathology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006706:Homeostasis / Q000502:physiology*; D006867:Hydrolases; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods; D008345:Manganese / Q000008:administration & dosage / Q000378:metabolism*; D009474:Neurons / Q000473:pathology*; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D008345:Manganese; D006867:Hydrolases; C527647:HsaD protein, Mycobacterium tuberculosis