title: CMY-20, a novel AmpC-type beta-lactamase from South African clinical Escherichia coli isolates.
abstract: In this study, we report the presence of a novel plasmid-mediated AmpC-type beta-lactamase that was isolated from 3 clinical Escherichia coli isolates at a tertiary teaching hospital in Durban, South Africa. The nucleotide sequence of the genes encoding this novel beta-lactamase was found to be >94% identical to the nucleotide sequences of the plasmid-mediated AmpC-type beta-lactamases originating from Citrobacter freundii. This enzyme differed from CMY-2 by 3 amino acid substitutions and was designated CMY-20.
authors.affiliation: School of Pharmacy and Pharmacology, University of KwaZulu-Natal, 4000 Durban, South Africa. mocktarc@ukzn.ac.za
authors.forename: Chunderika
authors.initials: C
authors.lastname: Mocktar
authors.forename: Usha
authors.initials: U
authors.lastname: Govinden
authors.forename: Adrian Willem
authors.initials: AW
authors.lastname: Sturm
authors.forename: Sabiha Yusuf
authors.initials: SY
authors.lastname: Essack
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D016971:Citrobacter freundii / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D006761:Hospitals; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013019:South Africa; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases