Pharmaceutical applications of isoelectric focusing on microchip with imaged UV detection.
For the first time, the application of a commercial Shimadzu microchip electrophoresis system MCE-2010 equipped with an imaging UV detector for isoelectric focusing (IEF) of therapeutic proteins is reported. By proper adjustment of the pH gradient, samples with pI values ranging from 2.85 to 10.3 can be focused to the imaged part of the separation channel. Three therapeutic proteins (hirudin, erythropoietin, and bevacizumab) have been successfully focused on the microchip, and the results have been compared to conventional capillary IEF in terms of peak profile, pI values, and reproducibility.
Vlcková
Markéta
M
Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
Kalman
Franka
F
Schwarz
Maria A
MA
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D061067:Antibodies, Monoclonal, Humanized; D000068258:Bevacizumab; D004921:Erythropoietin / Q000302:isolation & purification*; D006629:Hirudins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods*; D044085:Microfluidics; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D006629:Hirudins; D004921:Erythropoietin; D000068258:Bevacizumab