Production of human erythropoietin by chimeric chickens.
The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.
Kodama
Daisuke
D
Department of Biotechnology, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Nishimiya
Daisuke
D
Iwata
Ken-Ichi
K
Yamaguchi
Kazuhisa
K
Yoshida
Kazuhiro
K
Kawabe
Yoshinori
Y
Motono
Makoto
M
Watanabe
Hiroyuki
H
Yamashita
Takashi
T
Nishijima
Ken-Ichi
K
Kamihira
Masamichi
M
Iijima
Shinji
S
D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*
D004527:Egg Proteins; D011994:Recombinant Proteins; D004921:Erythropoietin