Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus.
Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mug MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxidant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxidant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxidant enzyme genes.
Sun
Yuhua
Y
Key Laboratory of Animal Development, Genetics and Reproduction of Education Ministry, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Tang
Rong
R
Li
Dapeng
D
Zhang
Xuzhen
X
Fu
Juan
J
Xie
Ping
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D004798:Enzymes / Q000235:genetics*; D006054:Goldfish / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D052998:Microcystins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D000975:Antioxidants; D004798:Enzymes; D052998:Microcystins