Printing protein arrays from DNA arrays.
We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
He
Mingyue
M
Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK.
Stoevesandt
Oda
O
Palmer
Elizabeth A
EA
Khan
Farid
F
Ericsson
Olle
O
Taussig
Michael J
MJ
D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*