title: (R)-albuterol decreases immune responses: role of activated T cells.
abstract: Racemic albuterol is an equimolar mixture of two isomers, (R) and (S). Whether (R) and (S) isomers and the combination of both exert different effects in immune activation is not well defined. We analyzed the effects of (R+S)-albuterol, (R)-albuterol and (S)-albuterol in a murine model of allergic pulmonary inflammation and in activated T cells. Mice (C57BL/6) sensitized and aerosol challenged with the allergen ovalbumin (OVA) or phosphate buffered saline (PBS) were treated with (R)-albuterol, (S)-albuterol or (R+S)-albuterol. Following administration of (R)-albuterol, allergen induced bronchoalveolar lavage eosinophils and IgE showed a decrease, albeit not significantly by ANOVA. As T cells are important in allergic inflammation, we asked whether (R+S), (R) or (S)-albuterol might differ in effects on T cells and on the activity of the inflammatory transcription factor NF-kappaB. In activated T cells, (R)-albuterol administration decreased levels of inflammatory cytokines and NF-kappaB activity. These studies suggest that (R)-albuterol decreases cytokine secretion and NF-kappaB activity in T cells.
authors.affiliation: Pulmonary and Critical Care Division, University of California San Diego, La Jolla, USA. marceferrada@yahoo.com
authors.forename: Marcela A
authors.initials: MA
authors.lastname: Ferrada
authors.forename: Erin L
authors.initials: EL
authors.lastname: Gordon
authors.forename: Kai Yu
authors.initials: KY
authors.lastname: Jen
authors.forename: Hong Zhen
authors.initials: HZ
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Leesa M
authors.initials: LM
authors.lastname: Barone
authors.forename: Sepideh
authors.initials: S
authors.lastname: Amirifeli
authors.forename: David L
authors.initials: DL
authors.lastname: Perkins
authors.forename: Patricia W
authors.initials: PW
authors.lastname: Finn
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D005260:Female; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000378:metabolism; D007249:Inflammation; D008171:Lung Diseases / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D010047:Ovalbumin; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D020033:Protein Isoforms / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
chemical_list: D000318:Adrenergic beta-Agonists; D016328:NF-kappa B; D020033:Protein Isoforms; D007073:Immunoglobulin E; D010047:Ovalbumin; D000420:Albuterol