Herbicide-induced anthocyanin accumulation in transgenic rice by expression of rice OSB2 under the control of rice CYP72A21 promoter.
CYP72A21, a rice cytochrome P450 gene, is induced by chloroacetamide herbicides. OSB2, a rice myc-type transcription factor, induces anthocyanin accumulation in rice leaves. To produce plants for biomonitoring by color change, we combined the CYP72A21 promoter and the OSB2 gene and introduced them into the rice isogenic line Taichung-65 CB A (T65), which contains loci CB and A from the rice cultivar Murasakiine. Leaves of the transgenic plants turned red upon treatment with the chloroacetamide herbicides acetochlor, alachlor, and metolachlor. Seedling shoots reddened upon treatment with alachlor or metolachlor at 10 microM, a concentration slightly higher than that used in the field. Anthocyanin content was increased approximately 200% by the treatment. The color changes were consistent with increased shoot expression of OSB2 and the anthocyanidin synthase gene (ANS). This system promises easy detection of rice plant gene expression. Transgenic plants could be used in the future to biomonitor accumulated herbicides.
Hirose
Sakiko
S
National Institute of Agrobiological Sciences, Ibaraki, Japan.
Kawahigashi
Hiroyuki
H
Tagiri
Akemi
A
Ohkawa
Yasunobu
Y
D000081:Acetamides / Q000737:chemistry; D000872:Anthocyanins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods*; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D006540:Herbicides / Q000737:chemistry*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000502:physiology; D014052:Toluidines / Q000737:chemistry
D000081:Acetamides; D000872:Anthocyanins; D051792:Basic Helix-Loop-Helix Proteins; D003577:Cytochrome P-450 Enzyme System; D006540:Herbicides; D010940:Plant Proteins; D014052:Toluidines; C443663:PLW-OSB2 protein, Oryza sativa; C000188:alachlor; C043377:acetochlor