The effect of cleft palate repair on contractile properties of single permeabilized muscle fibers from congenitally cleft goat palates.
Inherent differences in the levator veli palatini (LVP) muscle of cleft palates before palatoplasty may play a role in persistent postrepair velopharyngeal insufficiency (VPI). Contractile properties of LVP muscle fibers were analyzed from young (2-month) normal (YNP), young congenitally cleft (YCP) and again on the same YCP subjects 6 months after palatoplasty, mature repaired palate (MRP). The cross-sectional area and rate of force development (ktr) were measured. Specific force (sF(0)) and normalized power (nP(max)) were calculated. Using k(tr) to determine fiber type composition, YNP was 44% type 1 and 56% type 2, while YCP was 100% type 2. Two MRP subjects shifted to 100% type 1; 1 demonstrated increased resistance to fatigue. No differences in sF(0) were observed. nP(max) increased with presence of type 2 fibers. The persistent state of type 2 fibers following palatoplasty leads to increased fatigue in the LVP of MRP subjects and may cause VPI symptoms.
Hanes
Michael C
MC
Institute of Gerontology, University of Michigan, Ann Arbor, MI 48109-2200, USA.
Weinzweig
Jeffrey
J
Panter
Kip E
KE
McClellan
W Thomas
WT
Caterson
Stefanie A
SA
Buchman
Steven R
SR
Faulkner
John A
JA
Yu
Deborah
D
Cederna
Paul S
PS
Larkin
Lisa M
LM
D000818:Animals; D002972:Cleft Palate / Q000503:physiopathology* / Q000601:surgery; D004195:Disease Models, Animal; D006041:Goats; D007537:Isometric Contraction / Q000502:physiology; D009119:Muscle Contraction / Q000502:physiology*; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000502:physiology; D010156:Palatal Muscles / Q000503:physiopathology*; D010539:Permeability; D014681:Velopharyngeal Insufficiency / Q000473:pathology / Q000503:physiopathology