title: Anti-CeHV1 antibodies of two cynomolgus macaques cross-react with HSV2 but not HSV1 antigens in ELISA.
abstract: BACKGROUND
  The aim of the study was to compare the cross-reactivity of macaque anti-CeHV1 antibodies with type 1 and type 2 human herpes simplex viruses (HSV1 and HSV2).
  
  
  METHODS
  We studied the serum of 344 animals which had been tested either positive (n = 39) or negative (n = 305) for the presence of CeHV1 antibodies by expert laboratories. Macaque serums were studied by means of two ELISA: one based on HSV1 antigen-coated wells, the other on polystyrene beads coated with HSV1 and HSV2 antigens in approximately equal proportions.
  
  
  RESULTS
  In the serum of two animals originating from Vietnam, we found anti-CeHV1 antibodies cross-reacting with HSV2 but not with HSV1 antigens. For the serum with the highest titer, inhibition by soluble antigens confirmed the high affinity of the antibodies for HSV2 antigens.
  
  
  CONCLUSIONS
  Tests using HSV1 and HSV2 in a combined way are better suited to macaque screening than tests using only HSV1 antigens.
authors.affiliation: Laboratoire d'Immunogénétique Moléculaire, Université Paul Sabatier, Toulouse, France.
authors.forename: Edwin
authors.initials: E
authors.lastname: Coutrot
authors.forename: Marie
authors.initials: M
authors.lastname: Blancher-Sardou
authors.forename: Antoine
authors.initials: A
authors.lastname: Blancher
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D014744:Vietnam
chemical_list: D000914:Antibodies, Viral