title: Optimizing ultraperformance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design.
abstract: A rapid, sensitive, reproducible, and accurate ultraperformance liquid chromatographic (UPLC) method was developed for the simultaneous determination of 10 diterpenoid compounds (tanshinone I, tanshinone IIA, cryptotanshinone, dihydrotanshinone I, 1,2-dihydrotanshinquinone, methylenetanshinquinone, miltirone, 5,6-dehydrosugiol, sugiol, and przewalskin) in Salvia miltiorrhiza for the first time. Central composite design was applied as a powerful tool to optimize the most dominant parameters that influence the resolution of UPLC, that is, gradient, flow rate, and column temperature. Under optimum conditions, all peaks except 1,2-dihydrotanshinquinone and methylenetanshinquinone could be baseline separated within 8 min. Furthermore, the contents of these compounds in S. miltiorrhiza samples collected from different provinces of China have also been compared. The results showed that UPLC is one of the most efficient methods for the analysis of diterpenoid compounds in S. miltiorrhiza and that it is a potential method for quality control of this valuable traditional Chinese medicine.
authors.affiliation: Institute of Chinese Medical Sciences, University of Macau, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Xu
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Wang
authors.forename: Tai-Ping
authors.initials: TP
authors.lastname: Fan
authors.forename: Qin-Shi
authors.initials: QS
authors.lastname: Zhao
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004224:Diterpenes / Q000032:analysis*; D008516:Medicine, Chinese Traditional; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D004224:Diterpenes