Thermodesulfovibrio hydrogeniphilus sp. nov., a new thermophilic sulphate-reducing bacterium isolated from a Tunisian hot spring.
A new thermophilic sulphate-reducing bacterium (strain Hbr5T) was enriched and isolated from a terrestrial Tunisian hot spring. It was a non-spore-forming Gram-negative curved or vibrio-shaped bacterium. It appeared singly or in long chains and was actively motile by a polar flagellum. It possessed c-type cytochromes and desulfofuscidin. Growth occurred between 50 and 70 degrees C, with an optimum of 65 degrees C at pH 7.1. In the presence of sulphate as a terminal electron acceptor, this strain readily used H2 but formate only poorly. It could use sulphate, thiosulphate, sulphite or arsenate as electron acceptors. Its DNA G+C content was 36.1 mol%. Based on phenotypic, genomic, and phylogenetic characteristics, strain Hbr5T (=DSM 18151T, =JCM 13991T) is proposed to be assigned to a novel species of genus Thermodesulfovibrio, T. hydrogeniphilus sp. nov.
Haouari
Olfa
O
Laboratoire de Microbiologie IRD, UMR D180, Microbiologie et Biotechnologie des Environnements Chauds, IFR-BAIM, ESIL, Case 925. Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, F-13288 Marseille Cedex 09, France.
Fardeau
Marie-Laure
ML
Cayol
Jean-Luc
JL
Fauque
Guy
G
Casiot
Corinne
C
Elbaz-Poulichet
Françoise
F
Hamdi
Moktar
M
Ollivier
Bernard
B
D001149:Arsenates / Q000378:metabolism; D001482:Base Composition; D045304:Cytochromes c / Q000032:analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005407:Flagella; D005561:Formates / Q000378:metabolism; D020459:Genes, rRNA; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050885:Hydrogensulfite Reductase / Q000032:analysis; D008124:Locomotion; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial; D013431:Sulfates / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism; D014416:Tunisia
D001149:Arsenates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005561:Formates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D013447:Sulfites; C030544:formic acid; D006859:Hydrogen; D045304:Cytochromes c; D050885:Hydrogensulfite Reductase; C025657:arsenic acid