Molecular cloning and characterization of three distinct choriogenins in masu salmon, Oncorhynchus masou.
Three cDNAs, each encoding a different choriogenin (Chg), were isolated from a female masu salmon (Oncorhynchus masou) liver cDNA library. Two of the cDNA clones, Chg Halpha and Chg Hbeta, showed a close relationship and contained the typical domains of zona pellucida (ZP) B genes in fish, namely proline and glutamine rich repeats, a trefoil factor family domain, and a ZP domain. Specific antibodies against recombinant Chg H products (rmHalpha and rmHbeta) were generated to elucidate the relationship between the Chg H cDNAs and two types of serum Chg H protein, which were previously purified and characterized, and designated as very-high-molecular-weight vitelline envelope-related protein (vhVERP) and Chg H of masu salmon. The immunobiochemical analyses revealed that the Chg Halpha and Chg Hbeta clones encoded vhVERP and Chg H proteins, respectively. The third cDNA clone (Chg L) appeared to be a ZPC gene and, by mapping the N-terminal sequence of purified Chg L, was shown to encode serum Chg L protein. Various types of heteromultimer of the three Chgs were identified immunologically as high molecular weight chorion components, indicating the involvement of complex heterodimerization of multiple Chgs in the construction of chorion architecture in masu salmon.
Fujita
Toshiaki
T
Division of Marine Biosciences, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato, Hakodate, Hokkaido, Japan.
Fukada
Haruhisa
H
Shimizu
Munetaka
M
Hiramatsu
Naoshi
N
Hara
Akihiko
A
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004527:Egg Proteins / Q000235:genetics*; D005260:Female; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D017683:Oncorhynchus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011498:Protein Precursors / Q000235:genetics*; D011994:Recombinant Proteins / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D018076:DNA, Complementary; D004527:Egg Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins; C104950:choriogenin H