hnRNP-R regulates the PMA-induced c-fos expression in retinal cells.
This study focused on the function of hnRNP-R in the regulation of c-fos expression. We demonstrated that hnRNP-R accelerated the rise and decline phases of c-fos mRNAs and Fos proteins, allowing PMA to induce an augmented pulse response of c-fos expression. Then, we examined the role of the c-fos-derived AU-rich element (ARE) in hnRNP-R-regulated mRNA degradation. Studies with the ARE-GFP reporter gene showed that hnRNP-R significantly reduced the expression of GFP with an inserted ARE. Moreover, immunoprecipitation-RT-PCR analysis demonstrated that in R28 cells and rat retinal tissues, the c-fos mRNA was co-immunoprecipitated with hnRNP-R. These findings indicate that hnRNP-R regulates the c-fos expression in retinal cells, and that the ARE of c-fos mRNAs contributes to this regulation.
Huang
Jia
J
Laboratory of Genomic Physiology, Fudan University, 138 Yixueyuan Road, Shanghai, 200032, PR China.
Li
Shu-Jing
SJ
Chen
Xian-Hua
XH
Han
Yu
Y
Xu
Ping
P
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism*; D047468:Immunoprecipitation; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D038621:Regulatory Sequences, Ribonucleic Acid; D012160:Retina / Q000166:cytology* / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D034441:Heterogeneous-Nuclear Ribonucleoproteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D038621:Regulatory Sequences, Ribonucleic Acid; D049452:Green Fluorescent Proteins; D013755:Tetradecanoylphorbol Acetate