A possible mechanism of antitumor activity of 5(5',6'benzocoumaroyl-3')-methylaminouracil in vivo.
OBJECTIVE
To analyze possible mechanisms of anticancer activity of hydrobromide 5-(5',6'-benzocoumaroyl-3')-methylaminouracil (BCU) in vivo.


METHODS
BCU was administered at the dose of 6 mg/kg for seven days to rats bearing Guerin carcinoma (Gc). Interaction of BCU with DNA isolated from Gc cells was analyzed by electrophoresis and spectrophotometry.


RESULTS
It has been shown that BCU administration resulted in chromatin condensation, nuclei picnosis, increase of the DNase activity and the appearance of high-molecular DNA fragments in Gc cells.


CONCLUSIONS
BCU is shown to be able to interact directly with DNA molecules with the formation of stable complexes.
Kopylchuk
H P
HP
Department of Biochemistry, Y Fedkovych Chernivtsy National University, Chernivtsy, Ukraine.
Shmarakov
I O
IO
Marchenko
M M
MM
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects*; D005260:Female; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D051381:Rats; D014498:Uracil / Q000031:analogs & derivatives* / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D014498:Uracil