[Screening of Bacillus sp. strains producing thermostable alpha-amylase].
Fifteen thermostable alpha-amylases have been obtained as a result of screening of 76 Bacillus sp. strains, isolated from different natural substrates. They have shown 30-100% of their activities after 60 min of incubation at 100 degrees C. Seven thermostable a-amylases demonstrated 100% of their activities after 60 min of incubation at 100 degrees C. These enzymes showed maximal activities at pH 9.0 and exhibited 10-30% of activities at pH 11.0 and 12.0. These properties make them promising for future research and possible practical use. In this paper we have proposed a modified iodometric method for measurement and calculation of alpha-amylases activity. The influence of ammonium sulfate on protein concentration measurement by Bradford method was demonstrated and a necessity of dialysis after precipitation with ammonium sulfate was proposed.
Kubrak
O I
OI
Lushchak
V I
VI
D001407:Bacillus / Q000201:enzymology*; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D000516:alpha-Amylases / Q000737:chemistry / Q000302:isolation & purification*
D000516:alpha-Amylases