title: Intra-axonal translation and retrograde trafficking of CREB promotes neuronal survival.
abstract: During development of the nervous system, axons and growth cones contain mRNAs such as beta-actin, cofilin and RhoA, which are locally translated in response to guidance cues. Intra-axonal translation of these mRNAs results in local morphological responses; however, other functions of intra-axonal mRNA translation remain unknown. Here, we show that axons of developing mammalian neurons contain mRNA encoding the cAMP-responsive element (CRE)-binding protein (CREB). CREB is translated within axons in response to nerve growth factor (NGF) and is retrogradely trafficked to the cell body. In neurons that are selectively deficient in axonal CREB transcripts, increases in nuclear pCREB, CRE-mediated transcription and neuronal survival elicited by axonal application of NGF are abolished, indicating a signalling function for axonally synthesized CREB. These studies identify a signalling role for axonally derived CREB, and indicate that signal-dependent synthesis and retrograde trafficking of transcription factors enables specific transcriptional responses to signalling events at distal axons.
authors.affiliation: Department of Pharmacology, Weill Medical College, Cornell University, New York, NY 10021, USA.
authors.forename: Llewellyn J
authors.initials: LJ
authors.lastname: Cox
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hengst
authors.forename: Nadya G
authors.initials: NG
authors.lastname: Gurskaya
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
authors.forename: Samie R
authors.initials: SR
authors.lastname: Jaffrey
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D020439:Growth Cones / Q000502:physiology; D051379:Mice; D020932:Nerve Growth Factor / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D014176:Protein Biosynthesis / Q000502:physiology; D021381:Protein Transport; D051381:Rats
chemical_list: C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D020932:Nerve Growth Factor