Mouse embryonic diastema region is an ideal site for the development of ectopically transplanted tooth germ.
The anterior eye chamber and the kidney capsule of the mouse have been traditionally used for long-term culture of tooth germ grafts. However, although these sites provide an excellent growth environment, they do not represent real in situ sites for the development of a grafted tooth germ. Here, we describe a protocol to transplant a tooth germ into the mandibular diastema region of mouse embryos using exo utero surgery. Our results demonstrate that the mouse embryonic diastema region represents a normal physiological environment for the development of transplanted tooth germs. Transplanted tooth germs developed synchronically with and became indistinguishable from the endogenous ones. These ectopic teeth were vascularized and surrounded with nerve fibers, and were able to erupt normally. Thus, the exo utero transplantation approach will provide a new avenue to study tooth development and regeneration.
Song
Yiqiang
Y
Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana, USA.
Yan
Mingquan
M
Muneoka
Ken
K
Chen
YiPing
Y
D000818:Animals; D003970:Diastema / Q000196:embryology* / Q000601:surgery; D007150:Immunohistochemistry; D051379:Mice; D014083:Tooth Germ / Q000196:embryology* / Q000637:transplantation*