title: Expression and prognostic relevance of annexin A3 in prostate cancer.
abstract: OBJECTIVE
  By differential quantitative protein expression, it has previously been shown that annexin A3 (ANXA3) expression is associated with prostate cancer. However little is known about the role and biology of ANXA3 in the human prostate. The aim of this study was to thoroughly analyze ANXA3 expression patterns and its potential as a prognostic marker in a large set of benign, preneoplastic, and neoplastic prostate tissue samples.
  
  
  METHODS
  Immunohistochemistry-based ANXA3 protein expression was analyzed for 1589 prostate cancers as well as smaller subsets of benign epithelium and high-grade prostatic intraepithelial neoplasia (PIN) in a tissue microarray format.
  
  
  RESULTS
  All samples of benign prostatic epithelium and PIN showed ANXA3 protein expression, with PIN lesions showing a decreased staining intensity compared with benign epithelium (p<0.0001). In cancer, ANXA3 protein expression was essentially reduced, resulting in a negative staining rate of 27.2%, which correlated with increasing pT stage and Gleason score (p<0.0001). ANXA3 status in cancer was shown to be an independent adverse prognostic factor and enabled substratification of the large group of intermediate-risk patients (n=969) into high- and low-risk subgroups.
  
  
  CONCLUSIONS
  ANXA3 represents a promising candidate tissue marker, and when combined with the standard prognostic parameters, is suggested to provide a more precise prediction of prognosis in the individual patient, therefore harboring the potential to contribute to future patient management.
authors.affiliation: Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Köllermann
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schlomm
authors.forename: Holger
authors.initials: H
authors.lastname: Bang
authors.forename: Gerhard P
authors.initials: GP
authors.lastname: Schwall
authors.forename: Christoph
authors.initials: C
authors.lastname: von Eichel-Streiber
authors.forename: Ronald
authors.initials: R
authors.lastname: Simon
authors.forename: Martin
authors.initials: M
authors.lastname: Schostak
authors.forename: Hartwig
authors.initials: H
authors.lastname: Huland
authors.forename: Wigbert
authors.initials: W
authors.lastname: Berg
authors.forename: Guido
authors.initials: G
authors.lastname: Sauter
authors.forename: Helmut
authors.initials: H
authors.lastname: Klocker
authors.forename: André
authors.initials: A
authors.lastname: Schrattenholz
mesh_terms: D000368:Aged; D017318:Annexin A3 / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D017318:Annexin A3