Fibronectin adsorption on gold, Ti-, and Ta-oxide investigated by QCM-D and RSA modelling.
The adsorption of fibronectin on gold, Ti-, and Ta-oxide surfaces is investigated by means of the quartz crystal microbalance with dissipation (QCM-D) technique. The surface chemistry (gold, Ti-, and Ta-oxide) is found to influence the frequency shift observed during adsorption of the fibronectin layer with the magnitude being Delta f Au>Delta f Ti-oxide approximately Delta f Ta-oxide. Corresponding variations in the dissipation change normalised to frequency change (Delta D/Delta f) for the layer are observed. The QCM-D data are further analyzed by the random sequential adsorption (RSA) model, and adsorption rate parameter ka and footprint (a) determined, which supported the trend seen in the Delta f and Delta D/Delta f values. The value of ka found by the RSA modelling of the QCM-D resonance frequency data is found to match the ratio between the mass measured by QCM-D and the mass reported by optical techniques in literature. We conclude that comparison of the adsorption rate parameter (ka) obtained by RSA modelling of the QCM-D data with ka values obtained from RSA modelling of data obtained using optical techniques can be a route to determine the degree of hydration of the adsorbed protein layer.
Hemmersam
Anne Gry
AG
Interdisciplinary Nanoscience Center and Department of Physics and Astronomy, University of Aarhus, 8000 Aarhus C, Denmark.
Rechendorff
Kristian
K
Foss
Morten
M
Sutherland
Duncan S
DS
Besenbacher
Flemming
F
D000327:Adsorption; D005353:Fibronectins / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry; D013499:Surface Properties; D013635:Tantalum / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D005353:Fibronectins; D010087:Oxides; D011791:Quartz; D013635:Tantalum; D006046:Gold; D014025:Titanium; C078151:tantalum oxide