Quality considerations and selection of surface chemistry for glass-based DNA, peptide, antibody, carbohydrate, and small molecule microarrays.
The complexity of workflows for the production of high quality microarrays asks for the careful evaluation and implementation of materials and methods. As a cornerstone of the whole microarray process, the microarray substrate has to be chosen appropriately and a number of crucial considerations in respect to matching the research question with the technical requirements and possibilities have to be taken into account. In the following, how to lay the fundamental for high performance microarray experiments by evaluating basic quality requirements and the selection of suitable slide surface architectures for a variety of applications was concentrated.
Sobek
Jens
J
Functional Genomics Center Zurich, Switzerland.
Aquino
Catharine
C
Schlapbach
Ralph
R
D000906:Antibodies / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry*; D011786:Quality Control; D013499:Surface Properties
D000906:Antibodies; D002241:Carbohydrates; D010446:Peptide Fragments; D004247:DNA