title: Development and evaluation of a liquid chromatography-mass spectrometry assay and its application for the assessment of renal function.
abstract: In the present study we evaluated two commonly used iodinated contrast agents, iohexol and iodixanol, as potential markers of impaired renal function. A reversed phase LC-MS method has been developed in order to separate and quantify the two substances. The assay was linear between 0.02 and 9.7 micromol/L for iohexol and between 0.4 and 49.3 micromol/L for iodixanol (r(2) > 0.998). The recovery during sample preparation ranged from 89.1 to 112.4%. The intra- and inter-assay RSD values were 3.06-13.6% for iohexol and 4.32-12.7% for iodixanol. The validated method was subsequently applied to 17 patients scheduled for angiographic procedure displaying normal and impaired renal function. A mixture of iohexol and iodixanol was intra-arterially injected and their corresponding plasma levels were determined periodically over a 24h period following administration. The elimination of both contrast agents followed by the LC-MS approach allowed us to discriminate between patients with normal and impaired renal function at 4, 8 and 24h after administration. Our preliminary results support the predictive value of iohexol and/or iodixanol clearance in a clinical environment to screen and identify patients at risk of developing CIN.
authors.affiliation: Université de Sherbrooke, Faculté de Médecine et des Sciences de la santé, 3001, 12(e) Avenue Nord, Sherbrooke, Canada J1H 5N4.
authors.forename: M C
authors.initials: MC
authors.lastname: Denis
authors.forename: K
authors.initials: K
authors.lastname: Venne
authors.forename: D
authors.initials: D
authors.lastname: Lesiège
authors.forename: M
authors.initials: M
authors.lastname: Francoeur
authors.forename: S
authors.initials: S
authors.lastname: Groleau
authors.forename: M
authors.initials: M
authors.lastname: Guay
authors.forename: J
authors.initials: J
authors.lastname: Cusson
authors.forename: A
authors.initials: A
authors.lastname: Furtos
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003287:Contrast Media / Q000032:analysis* / Q000737:chemistry; D005919:Glomerular Filtration Rate; D006801:Humans; D007472:Iohexol / Q000032:analysis / Q000737:chemistry; D007677:Kidney Function Tests / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D014283:Triiodobenzoic Acids / Q000032:analysis / Q000737:chemistry
chemical_list: D003287:Contrast Media; D014283:Triiodobenzoic Acids; D007472:Iohexol; C044834:iodixanol