title: Purification and antitumour activity of a lipopeptide biosurfactant produced by Bacillus natto TK-1.
abstract: An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.
authors.affiliation: School of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China. caoxh@tust.edu.cn
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Cao
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Liao
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Cai
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Yang
authors.forename: Mei-Fang
authors.initials: MF
authors.lastname: Lu
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Huang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001407:Bacillus / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004435:Eating; D004789:Enzyme Activation / Q000187:drug effects; D005453:Fluorescence; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D055666:Lipopeptides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009821:Oils / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry
chemical_list: D000970:Antineoplastic Agents; D055666:Lipopeptides; D009821:Oils; D013501:Surface-Active Agents; D007770:L-Lactate Dehydrogenase; D053148:Caspase 3