title: The antibacterial efficacy of an aceraceous plant [Shantung maple (Acer truncatum Bunge)] may be related to inhibition of bacterial beta-oxoacyl-acyl carrier protein reductase (FabG).
abstract: Polyphenols, including flavonoids, are the major components of the extracts from aceraceous plants. They possess remarkable antibacterial and antitumour activity. Our study focused on whether the inhibition of the bacterial type II fatty acid synthesis system is the mechanism for the antibacterial effect of the related plant polyphenols. Extracts obtained from the fallen leaves of the Shantung maple (Acer truncatum Bunge) using different solvents, and the related pure compound PGG (1,2,3,4,6-penta-O-galloyl-beta-D-glucose), potently inhibited the FabG (beta-oxoacyl-ACP reductase) steps in the fatty-acid-elongation cycle with the IC(50) values between 0.9 and 7.2 microg/ml. An ethyl acetate extract appeared to inhibit FabG reductase in a mixed manner with NADPH, as did PGG with NADPH, demonstrating that they interfered with the binding of the cofactor to the enzyme. Gram-positive and Gram-negative bacteria and some fungi were used to evaluate the antibacterial abilities of different extract samples. The experiments showed that a higher polyphenol content of the extracts led to a more potent inhibitory capacity against FabG, thus enhancing the antibacterial efficacy.
authors.affiliation: Department of Chemical Biology, School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Shi-Yun
authors.initials: SY
authors.lastname: Luo
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Ye
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Zhao
authors.forename: Xu-Guang
authors.initials: XG
authors.lastname: Sun
authors.forename: Zhi-Qun
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Sun
authors.forename: Wei-Xi
authors.initials: WX
authors.lastname: Tian
authors.forename: Ying-Xia
authors.initials: YX
authors.lastname: Zhang
mesh_terms: D031002:Acer / Q000737:chemistry*; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001665:Binding Sites; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D000429:Alcohol Oxidoreductases; C023744:acetoacetyl-CoA reductase