[Herpes simplex virus-thymidine kinase gene transduced into T cell clone in the treatment of ulcerative colitis in rat].
OBJECTIVE
To investigate the effect of herpes simplex virus-thymidine kinase (HSV-TK) gene transduced into T cell clone on the treatment of ulcerative colitis (UC) in rat.


METHODS
The T cell clone transduced with HSV-tk was infused into 27 rats with UC. Changes of stimulation index (SI), CD4(+), CD8(+), IL-13, IL-4 were detected, and pathological changes before and after infusion was compared.


RESULTS
In the second and third day after tk+ T clone infusion, the inflammation of colon was assimilated. Two weeks later, the colon began to renovate and mend. The average SI was 7.39+/-1.24 before infusion, and 2.67+/-0.87 after infusion (P<0.05). Peripheral blood levels of CD4(+), CD8(+) or IL-13 and IL-4 in therapeutic group were significantly decreased as compared to control group (P<0.05).


CONCLUSIONS
HSV-tk gene transduced into T lymphocyte clone and infused back is effective for UC gene therapy and target design in rat.
Zhou
Jun
J
Department of Gastrointestinal Surgery, The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China.
Xu
Jun-yao
JY
Lan
Ping
P
Wang
Lei
L
Huang
Mei-jin
MJ
Wang
Jian-ping
JP
D000818:Animals; D003093:Colitis, Ulcerative / Q000628:therapy*; D041722:Genes, Transgenic, Suicide; D015316:Genetic Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018139:Simplexvirus / Q000235:genetics; D013601:T-Lymphocytes; D013937:Thymidine Kinase / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; D013937:Thymidine Kinase