title: Polarity in archaeal operon transcription in Thermococcus kodakaraensis.
abstract: An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential beta-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.
authors.affiliation: Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Santangelo
authors.forename: L'ubomíra
authors.initials: L
authors.lastname: Cubonová
authors.forename: Rie
authors.initials: R
authors.lastname: Matsumi
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Atomi
authors.forename: Tadayuki
authors.initials: T
authors.lastname: Imanaka
authors.forename: John N
authors.initials: JN
authors.lastname: Reeve
mesh_terms: D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D019848:Gene Expression Regulation, Archaeal; D019766:Genes, Archaeal / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009876:Operon / Q000235:genetics*; D019712:Thermococcus / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D019843:Archaeal Proteins; D006026:Glycoside Hydrolases