Development of an amperometric flow analysis sensor for specific detection of D-psicose.
The aim of this study was to develop a simple, rapid and highly sensitive sensor for measuring the rare sugar d-psicose. The proposed system adopts amperometric flow analysis and two consecutive enzyme reactions consisting of a reactor packed with d-tagatose 3-epimerase (DTE)-immobilized beads, which converts d-psicose to d-fructose, and a carbon-paste electrode containing d-fructose dehydrogenase (DFDH). In order to fabricate a robust sensor system, various experimental parameters were optimized including the buffer composition, flow rate for the two enzyme reactions and the size of micro-flow cell. The developed sensor responded linearly to d-psicose concentration in the range from 0.08 to 50mM (R(2)=0.988). The signal/noise ratio was 3.0 for the 0.08 mM d-psicose solution, and the relative standard deviations were 1.7 (n=20) and 2.6% (n=20) for the 10 and 20mM d-psicose solutions, respectively. One round of assay was completed within 8 min. Our results suggest that the sensor can be used not only for the detection of d-psicose in food samples but also for monitoring d-psicose within the environment. Moreover, the sensor system can be applied to the detection of many other rare sugars by using the same measurement principle.
Miyanishi
Nobumitsu
N
Department of Functional Glycomics, Life Science Research Center, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
Sato
Naruhide
N
Nakakita
Shin-Ichi
S
Sumiyoshi
Wataru
W
Morimoto
Kenji
K
Okuma
Hirokazu
H
Tokuda
Masaaki
M
Izumori
Ken
K
Watanabe
Etsuo
E
Hirabayashi
Jun
J
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry; D017022:Flow Injection Analysis / Q000295:instrumentation*; D005632:Fructose / Q000032:analysis*; D015203:Reproducibility of Results
C003243:psicose; D005632:Fructose