title: Distinctive gene transduction efficiencies of commonly used viral vectors in the retina.
abstract: The transduction efficiency and cell tropism of viral vectors rAAV2/1, rAAV2, Ad5, Ad5/F35, and Lentivirus were evaluated in retina. All viral vectors achieved efficient transduction in living rat retina. However, each vector showed distinctive efficiency in vitro especially for rAAV2/1, which displayed poor transduction in cultured retinal cells. Distinctive cell-specific GFP expression was observed in vivo and in vitro for the same viral vector. The cell-specific tropism was not strictly correlated with the correspondent distribution of viral receptors in retina. These results provided important insights into the selection of appropriate vectors when specific retinal diseases are considered for gene therapy.
authors.affiliation: Experimental Research Center, No.1 People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Sheng-Hai
authors.initials: SH
authors.lastname: Zhang
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Wu
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Wu
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Dong
authors.forename: Xin-Jian
authors.initials: XJ
authors.lastname: Liu
authors.forename: Chuan-Yuan
authors.initials: CY
authors.lastname: Li
authors.lastname: Qian-Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000378:metabolism; D051916:Membrane Cofactor Protein / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011991:Receptors, Virus / Q000378:metabolism; D019030:Receptors, Vitronectin / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D014161:Transduction, Genetic; D018523:Tropism
chemical_list: C568771:CLMP protein, human; C568773:Clmp protein, rat; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019812:Heparan Sulfate Proteoglycans; D016023:Integrins; D051916:Membrane Cofactor Protein; D011991:Receptors, Virus; D019030:Receptors, Vitronectin; C064660:integrin alphaVbeta5; D049452:Green Fluorescent Proteins