Gold nanostoves for microsecond DNA melting analysis.
In traditional DNA melting assays, the temperature of the DNA-containing solution is slowly ramped up. In contrast, we use 300 ns laser pulses to rapidly heat DNA bound gold nanoparticle aggregates. We show that double-stranded DNA melts on a microsecond time scale that leads to a disintegration of the gold nanoparticle aggregates on a millisecond time scale. A perfectly matching and a point-mutated DNA sequence can be clearly distinguished in less than one millisecond even in a 1:1 mixture of both targets.
Stehr
Joachim
J
Photonics and Optoelectronics Group, Physics Department, CeNS, Ludwig-Maximilians-Universität München, Amalienstrasse 54, 80799 Munich, Germany.
Hrelescu
Calin
C
Sperling
Ralph A
RA
Raschke
Gunnar
G
Wunderlich
Michael
M
Nichtl
Alfons
A
Heindl
Dieter
D
Kürzinger
Konrad
K
Parak
Wolfgang J
WJ
Klar
Thomas A
TA
Feldmann
Jochen
J
D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D017422:Sequence Analysis, DNA / Q000379:methods*; D013499:Surface Properties; D044366:Transition Temperature
D046911:Macromolecular Substances; D006046:Gold; D004247:DNA