Role of RAD51 in the repair of MuDR-induced double-strand breaks in maize (Zea mays L.).
Rates of Mu transposon insertions and excisions are both high in late somatic cells of maize. In contrast, although high rates of insertions are observed in germinal cells, germinal excisions are recovered only rarely. Plants doubly homozygous for deletion alleles of rad51A1 and rad51A2 do not encode functional RAD51 protein (RAD51-). Approximately 1% of the gametes from RAD51+ plants that carry the MuDR-insertion allele a1-m5216 include at least partial deletions of MuDR and the a1 gene. The structures of these deletions suggest they arise via the repair of MuDR-induced double-strand breaks via nonhomologous end joining. In RAD51- plants these germinal deletions are recovered at rates that are at least 40-fold higher. These rates are not substantially affected by the presence or absence of an a1-containing homolog. Together, these findings indicate that in RAD51+ germinal cells MuDR-induced double-strand breaks (DSBs) are efficiently repaired via RAD51-directed homologous recombination with the sister chromatid. This suggests that RAD51- plants may offer an efficient means to generate deletion alleles for functional genomic studies. Additionally, the high proportion of Mu-active, RAD51- plants that exhibit severe developmental defects suggest that RAD51 plays a critical role in the repair of MuDR-induced DSBs early in vegetative development.
Li
Jin
J
Department of Genetics, Development, and Cell Biology, Center for Plant Genomics, Iowa State University, Ames, Iowa 50011, USA.
Wen
Tsui-Jung
TJ
Schnable
Patrick S
PS
D000483:Alleles; D001483:Base Sequence; D003433:Crosses, Genetic; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D004251:DNA Transposable Elements / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D051135:Rad51 Recombinase / Q000378:metabolism*; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D003313:Zea mays / Q000235:genetics* / Q000254:growth & development
D004251:DNA Transposable Elements; D051135:Rad51 Recombinase