Simplified rat lung transplantation by using a modified cuff technique.
Mizuta's cuff technique in rat lung transplantation (LT) model has some disadvantages, such as twisting of blood vessels or bronchus and being time-consuming, which complicate procedures for anastomosis. This study was performed to investigate the advantage of using a simplified cuff technique in LT. The anastomosis time was compared in two groups. In group I, Mizuta'scuff technique was performed in 50 rat orthotopic left lung transplants. In group II, a simple modified cuff technique was performed in 48 rat orthotopic left lung transplants. The successful rate of the new technique for anastomosis was 100%. No twist of vessels or bronchus and no bleeding or air leakage were observed in group II. The anastomosis time of group II was significantly less than for group I (11.2 +/- 2.1 min vs. 18.1 +/- 3.6 min, mean +/- SD, p < .01). This simple modified cuff technique led to less anastomosis time and avoided potential complications induced by the cuff-tail technique. It has been verified to be a safe, simple, and reproducible technique that can provide us with a more precise assessment in the rat LT model.
Zhai
Wei
W
Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jie Fang Avenue #1277, Wuhan, China. zhaiwei720308@hotmail.com
Ge
Jing
J
Inci
Ilhan
I
Hillinger
Sven
S
Markus
Cardell
C
Korom
Stephan
S
Weder
Walter
W
D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D001980:Bronchi / Q000601:surgery; D005549:Foreign-Body Reaction / Q000517:prevention & control; D016040:Lung Transplantation / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors; D054159:Torsion, Mechanical