Novel cellulases from an extremophilic filamentous fungi Penicillium citrinum: production and characterization.
The enzymatic hydrolysis of cellulose has potential economical and environment-friendly applications. Therefore, discovery of new extremophilic cellulases is essential to meet the requirements of industry. Penicillium citrinum (MTCC 6489) that was previously isolated from soil in our laboratory, produced alkali tolerant and thermostable cellulases. Endoglucanase and filter paper activity hydrolase (FPAse) production of P. citrinum were studied using wheat bran substrate in solid state and submerged culture. Zymogram analysis of endoglucanase revealed the presence of two isoforms differing in molecular weight. One of them was 90 kDa and other one was 38 kDa. Partially purified endoglucanase showed two different peaks at pH 5.5 and 8.0, respectively, in its pH optima curve. But FPase showed only one peak (at pH 6.5) in its pH optima curve. Cellulase of P. citrinum is thermostable in nature. The present work reports for the first time, the alkali stable cellulase from alkali tolerant fungus Penicillium citrinum. Thermostable endoglucanase from P. citrinum may have potential effectiveness as additives to laundry detergents.
Dutta
Tanmay
T
Department of Biochemistry, University College of Science, Calcutta University, 35, Ballygunge Circular Road, Kolkata 700 019, West Bengal, India.
Sahoo
Rupam
R
Sengupta
Rajib
R
Ray
Sougata Sinha
SS
Bhattacharjee
Arindam
A
Ghosh
Sanjay
S
D044602:Cellulases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008970:Molecular Weight; D010407:Penicillium / Q000201:enzymology* / Q000302:isolation & purification; D020033:Protein Isoforms / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature
D003470:Culture Media; D005656:Fungal Proteins; D020033:Protein Isoforms; D044602:Cellulases