Application of an indirect immunofluorescent staining method for detection of Salmonella enteritidis in paraffin slices and antigen location in infected duck tissues.
OBJECTIVE
To detect Salmonella enteritidis (S. enteritidis) in paraffin slices and antigen location in infected duck tissues.


METHODS
The rabbits were immunized with purified bacillus to obtain S. enteritidis-specific antibody, which were then extracted by the caprylic-ammonium sulphate method, purified through High-Q columns. An indirect immuno-fluorescent staining method (IFA) was established to detect the S. enteritidis antigen in paraffin slices. Detected S. enteritidis in each organ tissue of ducklings experimentally infected with S. enteritidis.


RESULTS
The gland of Garder, heart, kidney, spleen, liver, brain, ileum, jejunum, bursa of Fabricius from S. enteritidis experimentally infected ducklings were positive or strongly positive, and the S. enteritidis antigen mainly distributed in the infected cell cytoplasm.


CONCLUSIONS
IFA is an intuitionist, sensitive and specific method in detecting S. enteritidis antigen in paraffin wax slices, and it is a good method in diagnosis and antigen location of S. enteritidis. We also conclude that the gland of Garder, heart, kidney, spleen, liver, ileum, jejunum are target organs in S. enteritidis infections of duck, and S. enteritidis is an intracellular parasitic bacterium.
Yan
Bin
B
Avian Disease Research Center, Collage of Veterinary Medicine of Sichuan Agriculture University, Yaan 625014, Sichuan Province, China.
Cheng
An-Chun
AC
Wang
Ming-Shu
MS
Deng
Shu-Xuan
SX
Zhang
Zhen-Hua
ZH
Yin
Nian-Chun
NC
Cao
Ping
P
Cao
Sheng-Yan
SY
D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D004372:Ducks; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D016612:Paraffin Embedding; D011201:Poultry Diseases / Q000382:microbiology* / Q000473:pathology; D012480:Salmonella Infections / Q000175:diagnosis* / Q000473:pathology; D012477:Salmonella enteritidis / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods
D000942:Antigens, Bacterial