title: Angiotensin-converting enzyme in Spodoptera littoralis: molecular characterization, expression and activity profile during development.
abstract: The characterization of the full-length angiotensin-converting enzyme (ACE) cDNA sequence of the lepidopteran Spodoptera littoralis is reported in this study. The predicted open reading frame encodes a 647 amino acids long protein (SlACE) and shows 63.6% identity with the Bombyx mori ACE sequence. A 3D-model, consisting of 26 alpha-helices and three beta-sheets, was predicted for the sequence. SlACE expression was studied in the embryonic, larval and pupal stages of S. littoralis and in different tissues of the last larval stage by reverse-transcribed PCR. This revealed that the gene is expressed throughout the life cycle and especially in brain, gut and fat body tissue of the last stage. These results are in agreement with a role of ACE in the metabolism of neuropeptides and gut hormones. In addition, ACE activity has been studied in more detail during development, making use of a fluorescent assay. High ACE peptidase activity coincides with every transition state, from embryo to larva, from larva to larva and from larva to pupa. A peak value in activity occurs during the early pupal stage. These results indicate the importance of SlACE during metamorphosis and reveal the high correlation of ACE activity with the insect's development, which is regulated by growth and developmental hormones.
authors.affiliation: Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium. els.lemeire@ugent.be
authors.forename: Els
authors.initials: E
authors.lastname: Lemeire
authors.forename: Bartel
authors.initials: B
authors.lastname: Vanholme
authors.forename: Thomas
authors.initials: T
authors.lastname: Van Leeuwen
authors.forename: John
authors.initials: J
authors.lastname: Van Camp
authors.forename: Guy
authors.initials: G
authors.lastname: Smagghe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018507:Gene Expression Regulation, Developmental; D007814:Larva / Q000378:metabolism; D008969:Molecular Sequence Data; D010063:Ovum / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism*; D011679:Pupa / Q000378:metabolism; D017422:Sequence Analysis, DNA; D018411:Spodoptera / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D007703:Peptidyl-Dipeptidase A