title: Expressed sequence tag analysis and development of gene associated markers in a near-isogenic plant system of Eragrostis curvula.
abstract: Eragrostis curvula (Schrad.) Nees is a forage grass native to the semiarid regions of Southern Africa, which reproduces mainly by pseudogamous diplosporous apomixis. A collection of ESTs was generated from four cDNA libraries, three of them obtained from panicles of near-isogenic lines with different ploidy levels and reproductive modes, and one obtained from 12 days-old plant leaves. A total of 12,295 high-quality ESTs were clustered and assembled, rendering 8,864 unigenes, including 1,490 contigs and 7,394 singletons, with a genome coverage of 22%. A total of 7,029 (79.11%) unigenes were functionally categorized by BLASTX analysis against sequences deposited in public databases, but only 37.80% could be classified according to Gene Ontology. Sequence comparison against the cereals genes indexes (GI) revealed 50% significant hits. A total of 254 EST-SSRs were detected from 219 singletons and 35 from contigs. Di- and tri- motifs were similarly represented with percentages of 38.95 and 40.16%, respectively. In addition, 190 SNPs and Indels were detected in 18 contigs generated from 3 to 4 libraries. The ESTs and the molecular markers obtained in this study will provide valuable resources for a wide range of applications including gene identification, genetic mapping, cultivar identification, analysis of genetic diversity, phenotype mapping and marker assisted selection.
authors.affiliation: Centro de Recursos Naturales Renovables de la Zona Semiárida-CONICET, Camino de La Carrindanga Km 7.0, Bahia Blanca, Argentina.
authors.forename: Gerardo D L
authors.initials: GD
authors.lastname: Cervigni
authors.forename: Norma
authors.initials: N
authors.lastname: Paniego
authors.forename: Marina
authors.initials: M
authors.lastname: Díaz
authors.forename: Juan P
authors.initials: JP
authors.lastname: Selva
authors.forename: Diego
authors.initials: D
authors.lastname: Zappacosta
authors.forename: Darío
authors.initials: D
authors.lastname: Zanazzi
authors.forename: Iñaki
authors.initials: I
authors.lastname: Landerreche
authors.forename: Luciano
authors.initials: L
authors.lastname: Martelotto
authors.forename: Silvina
authors.initials: S
authors.lastname: Felitti
authors.forename: Silvina
authors.initials: S
authors.lastname: Pessino
authors.forename: Germán
authors.initials: G
authors.lastname: Spangenberg
authors.forename: Viviana
authors.initials: V
authors.lastname: Echenique
mesh_terms: D031750:Eragrostis / Q000235:genetics*; D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics; D015723:Gene Library; D005819:Genetic Markers; D018515:Plant Leaves / Q000235:genetics; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers