[Identification of Leptospira interrogans serovar pomona in mice genetically selected for high and low antibody production, by means of the immunoperoxidase technique in renal tissue and bacterial isolation in Fletcher medium].
The present work had the objective of identifying the presence of Leptospira interrogans serovar pomona in mice that had been genetically selected for high and low response to antibodies. All the animals were subjected to bacterial isolation, immunohistochemical analysis (immunoperoxidase) in renal tissue sections and hematoxylin-eosin staining. The immunoperoxidase technique was little more sensitive than culturing, but both were good parameters for agent identification. More severe renal lesions were present at times when there were greater numbers of bacteria isolated in culture medium. Mice of the lineage HIV-A were able to eliminate the Leptospira more efficiently and faster than the lineage LIV-A could. However, the study demonstrated that both lineages of the IV-A selection were efficient in controlling the infectious process.
Haanwinckel
Maria Cristina Santos
MC
Departamento de Doenças Tropicais e Diagnóstico por Imagem, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP.
Cavalheiro
Juliana Semin
JS
Oliveira
Silvio Luis de
SL
D000818:Animals; D003470:Culture Media; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D054884:Host-Pathogen Interactions; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000382:microbiology* / Q000473:pathology; D041401:Leptospira interrogans serovar pomona / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013997:Time Factors
D003470:Culture Media; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin