Expression of senescence-associated beta-galactosidase (SA-beta-Gal) by human skin fibroblasts, effect of advanced glycation end-products and fucose or rhamnose-rich polysaccharides.
Expression by cells of the SA-beta-Gal was shown to be a reliable indicator of the switch mechanism used by cells to enter the senescent phenotype. We used this method in order to explore the variation of SA-beta-Gal-positive cells with passage number and time spent in culture. Both parameters produced an increase of SA-beta-Gal-positive cells. The addition of a Maillard-product (advanced glycation end-product=AGE) to the fibroblast cultures also increased SA-beta-Gal expression. Fucose- and rhamnose-rich oligo- and polysaccharides (FROPs and RROPs, respectively) provided a significant protection against this AGE-induced increase of SA-beta-Gal-positive cells. It is speculated that these processes might well play an important role in skin aging.
Ravelojaona
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Laboratoire de Recherches Ophtalmologiques, Hôtel Dieu, Université Paris, France.
Robert
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AM
Robert
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D002460:Cell Line; D005347:Fibroblasts / Q000378:metabolism*; D005643:Fucose / Q000502:physiology; D017127:Glycation End Products, Advanced / Q000502:physiology*; D006801:Humans; D011134:Polysaccharides / Q000502:physiology; D012210:Rhamnose / Q000502:physiology; D015595:Skin Aging / Q000502:physiology*; D001616:beta-Galactosidase / Q000378:metabolism*
D017127:Glycation End Products, Advanced; D011134:Polysaccharides; D005643:Fucose; D001616:beta-Galactosidase; D012210:Rhamnose