Blood screening for influenza.
Influenza viruses, including highly pathogenic avian influenza virus (H5N1), could threaten blood safety. We analyzed 10,272 blood donor samples with a minipool nucleic acid amplication technique. Analytical sensitivity of the method was 804 geq/mL and 444 geq/mL for generic influenza primers and influenza (H5N1) subtype-specific primers. This study demonstrates that such screening for influenza viruses is feasible.
Hourfar
Michael Kai
MK
German Red Cross, Frankfurt, Germany.
Themann
Anna
A
Eickmann
Markus
M
Puthavathana
Pilaipan
P
Laue
Thomas
T
Seifried
Erhard
E
Schmidt
Michael
M
D001483:Base Sequence; D001769:Blood / Q000821:virology*; D001782:Blood Donors; D003429:Cross Reactions; D005784:Gene Amplification; D005858:Germany; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000097:blood* / Q000635:transmission*; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D015203:Reproducibility of Results; D018570:Risk Assessment; D012449:Safety; D012680:Sensitivity and Specificity; D013045:Species Specificity