Separation of aromatic acids by wide-bore electrophoresis with nanoparticles prepared by electrospray as pseudostationary phase.
A model mixture of six aromatic acids has been separated using a laboratory-made wide-bore electrophoretic device with aminopropyl-modified nanoparticles used as pseudostationary phase. Optimization of preparation of nanoparticles by an electrospray (ES) method is described. With the optimized electrophoretic method, 30 mmol/L acetate running buffer, pH 4.5, containing 1.0 mg/mL of nanoparticles as an additive was used, and 3.0 kV applied potential, improved resolution was achieved. The average theoretical plate number obtained was above 5.0 x 10(4) theoretical plates per meter with the highest value achieved in certain runs exceeding 1.0 x 10(5) theoretical plates per meter, which was better than previously reported results (approximately 6.7 x 10(4) theoretical plates per meter). Furthermore, repeatabilities of 2, 6.5, and 6% were obtained for the migration time, peak areas, and peak height, respectively. Additionally, sample capacity and sensitivity were improved by hundredfold using the novel wide-bore electrophoresis system compared to traditional CE.
Liu
Dan-ning
DN
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P. R. China.
Wang
Jing
J
Guo
Yu-gao
YG
Yuan
Rui-juan
RJ
Wang
Huai-feng
HF
Bao
James J
JJ
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009930:Organic Chemicals / Q000032:analysis*
D002021:Buffers; D009930:Organic Chemicals