title: Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.
abstract: Pro- and mature BDNF activate very different receptors and intracellular pathways, potentially leading to either neuronal death or survival. Here we examined the biochemistry of endogenous BDNF in mouse neurons using sensitive reagents and found that pro-BDNF is rapidly converted intracellularly to mature BDNF, the latter being stored and released by excitatory input.
authors.affiliation: Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Stefanie
authors.initials: S
authors.lastname: Rauskolb
authors.forename: Martin
authors.initials: M
authors.lastname: Polack
authors.forename: Johannes
authors.initials: J
authors.lastname: Klose
authors.forename: Roland
authors.initials: R
authors.lastname: Kolbeck
authors.forename: Martin
authors.initials: M
authors.lastname: Korte
authors.forename: Yves-Alain
authors.initials: YA
authors.lastname: Barde
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000172:deficiency / Q000378:metabolism*; D019556:COS Cells; D002522:Chlorocebus aethiops; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006624:Hippocampus / Q000166:cytology*; D047468:Immunoprecipitation / Q000379:methods; D017774:Long-Term Potentiation / Q000502:physiology / Q000528:radiation effects; D008715:Methionine / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011498:Protein Precursors / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology / Q000528:radiation effects; D014162:Transfection / Q000379:methods; D014316:Tritium / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D011498:Protein Precursors; C426674:brain-derived neurotrophic factor precursor; D014316:Tritium; D008715:Methionine