Alleviation of ischemia/reperfusion injury in ob/ob mice by inhibiting UCP-2 expression in fatty liver.
OBJECTIVE
To investigate the protective effect of target suppression of uncoupling protein-2 (UCP-2) on ischemia/reperfusion (I/R) injury in fatty liver in ob/ob mice.


METHODS
Plasmids suppressing UCP-2 expression were constructed, and transfected into fatty liver cells cultured in vitro and the ob/ob mouse I/R injury model. Serum tumor necrosis factor (TNF)- alpha levels, UCP-2 mRNA expression, alanine aminotransferase (ALT) levels in ob/ob mice were tested, and the pathological changes in fatty liver were observed in experimental and control groups.


RESULTS
In ob/ob mouse I/R models, serum TNF-alpha levels were significantly higher than in normal controls. After the plasmids were transfected into the cultured cells and animal models, expression of UCP-2 mRNA was significantly reduced as compared with that in the control group (2(1.56+/-0.15) vs 2(-0.45+/-0.15), P<0.05). In ob/ob mouse models, in which expression of UCP-2 was suppressed, serum ALT levels were significantly lower than those of other groups, and pathological analysis revealed that injury of liver tissues was significantly alleviated.


CONCLUSIONS
The target suppression of UCP-2 expression in fatty liver can alleviate the I/R injury in the ob/ob mice.
Wan
Chi-Dan
CD
Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. chidanwan@163.com
Wang
Chun-You
CY
Liu
Tao
T
Cheng
Rui
R
Wang
Hong-Bo
HB
D000818:Animals; D005234:Fatty Liver / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D007473:Ion Channels / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D024101:Mitochondrial Proteins / Q000235:genetics*; D010957:Plasmids / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000503:physiopathology / Q000628:therapy*; D014162:Transfection; D000071246:Uncoupling Protein 2
D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2