Inhibition of gene expression directed by small interfering RNAs in infectious bronchitis virus.
In this study, fourteen small interfering RNAs (siRNAs) were synthesized for targeting of coding gene sequences, specific 5'-leader sequence of mRNA and 3'-end sequence of the Infectious bronchitis virus (IBV) genome. The expression of viral genes in Vero-E6 cells was detected by real-time PCR. Obtained results indicated that the majority of siRNAs could effectively inhibit the expression of viral genes. The inhibition effect of siRNAs significantly differed among various genes and sites on the virus genome.
Meng
H
H
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, PR China.
Cui
F Y
FY
Chen
X H
XH
Pan
Y C
YC
D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002522:Chlorocebus aethiops; D015870:Gene Expression; D016679:Genome, Viral; D001351:Infectious bronchitis virus / Q000235:genetics / Q000254:growth & development*; D034741:RNA, Small Interfering / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D034741:RNA, Small Interfering; D012367:RNA, Viral