Glyconanoparticle-DNA interactions: an atomic force microscopy study.
Glyconanoparticles which present carbohydrate and amino groups motifs at their surface were produced. These particles were highly stable and soluble in aqueous solutions. The presence of the carbohydrate groups also allowed the inclusion of more strongly binding groups, without affecting solubility. The binding of a model DNA, plasmid by these nanoparticles was studied by atomic force microscopy, transmission electron microscopy, and gel electrophoresis. Significant differences between the nanoparticles based on their affinities for the DNA were found, with implications for their potential use as nonviral gene delivery agents.
Eaton
Peter
P
Laboratory of Glyconanotechnology, IIQ-CSIC, 41092 Seville, Spain. peter.eaton@fc.up.pt
Ragusa
Andrea
A
Clavel
Caroline
C
Rojas
Cristina T
CT
Graham
Paul
P
Durán
Raúl V
RV
Penadés
Soledad
S
D015342:DNA Probes / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D006023:Glycoproteins / Q000737:chemistry*; D006046:Gold; D006601:Hexoses / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D009690:Nucleic Acid Conformation; D012996:Solutions
D015342:DNA Probes; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D006601:Hexoses; D012996:Solutions; D006046:Gold