S-adenosylmethionine inhibits ubiquitin-proteasome system in vitro and on rat vascular smooth muscle cells.
S-adenosylmethionine is a metabolite regulating many biological processes; S-adenosylmethionine effect on ubiquitin-proteasome system (UPS) has not been studied yet. We investigated S-adenosylmethionine effects on UPS activity both in vitro, by inhibitor screening assay, and in rat vascular smooth muscle cells, by Western Blot of proteasomal targets. We found that S-adenosylmethionine inhibited UPS activity.
D'Anselmi
Fabrizio
F
Department of Surgery P.Valdoni, University of Rome La Sapienza, Italy. Fabrizio.Danselmi@uniroma1.it
Cucina
Alessandra
A
Cavallaro
Giuseppe
G
Bizzarri
Mariano
M
Cavallaro
Rosaria Adele
RA
Fuso
Andrea
A
Cavallaro
Antonino
A
Scarpa
Sigfrido
S
D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000201:enzymology; D002478:Cells, Cultured; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000201:enzymology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D061988:Proteasome Inhibitors; D051381:Rats; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D025801:Ubiquitin / Q000037:antagonists & inhibitors* / Q000378:metabolism
D061988:Proteasome Inhibitors; D025801:Ubiquitin; D012436:S-Adenosylmethionine; D046988:Proteasome Endopeptidase Complex