Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients.
We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.
Wölfel
Roman
R
Bundeswehr Institute of Microbiology, Munich, Germany.
Paweska
Janusz T
JT
Petersen
Nadine
N
Grobbelaar
Antoinette A
AA
Leman
Patricia A
PA
Hewson
Roger
R
Georges-Courbot
Marie-Claude
MC
Papa
Anna
A
Günther
Stephan
S
Drosten
Christian
C
D014644:Genetic Variation; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000097:blood / Q000175:diagnosis* / Q000401:mortality; D006801:Humans; D010802:Phylogeny; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D019562:Viral Load