title: Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice.
abstract: A specific and sensitive high-performance liquid chromatographic (HPLC) method with photodiode-array (PDA) ultraviolet detection was developed for the simultaneous determination of three bioactive constituents of Cedrus deodara namely wikstromol, matairesinol and dibenzylbutyrolactol in mouse plasma. In solid-phase extraction (SPE) these constituents were successfully separated using a C18 column by isocratic elution using acetonitrile:water containing hexanesulphonic acid, 32:68 (v/v). The flow rate was set at 1ml/min and detector wavelength at 225nm. Good linearity (r2>0.999) was observed over the studied range of 0.015-5.0microg/ml for wikstromol and 0.030-5.0microg/ml for matairesinol and dibenzylbutyrolactol. The CV values of intra-day precision for wikstromol, matairesinol and dibenzylbutyrolactol were in between 1.8-6.9, 1.7-4.9 and 1.6-4.2% and values of inter-day precision were in between 10.4-12.2, 9.7-11 and 10-11.2%, respectively. The extraction recoveries at low to high concentration were greater than 98, 83 and 87% for each analyte, respectively. The LOQ for wikstromol was 0.015microg/ml and for both matairesinol and dibenzylbutyrolactol it was 0.030microg/ml. The developed method was used to determine the pharmacokinetics of the three analytes in mice after intraperitoneal administration of CD-3.
authors.affiliation: Division of Pharmacology, Indian Institute of Integrative Medicine (Formerly Regional Research Laboratory), CSIR, Canal Road, Jammu Tawi 180001, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Sachin
authors.forename: M
authors.initials: M
authors.lastname: Koul
authors.forename: A
authors.initials: A
authors.lastname: Zutshi
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.forename: A K
authors.initials: AK
authors.lastname: Tikoo
authors.forename: M K
authors.initials: MK
authors.lastname: Tikoo
authors.forename: A K
authors.initials: AK
authors.lastname: Saxena
authors.forename: S C
authors.initials: SC
authors.lastname: Sharma
authors.forename: R K
authors.initials: RK
authors.lastname: Johri
mesh_terms: D000818:Animals; D028203:Cedrus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000493:pharmacokinetics*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D010936:Plant Extracts