Microfluidic devices for studying growth and detachment of Staphylococcus epidermidis biofilms.
Microfluidic devices were used to study the influences of hydrodynamics of local microenvironments on Staphylococcus epidermidis (S. epidermidis) biofilm formation and the effects of a poly(beta-1,6-N-acetyl glucosamine)-hydrolyzing enzyme (dispersin B) and/or an antibiotic (rifampicin) on the detachment of the biofilm. Elongated, monolayered biofilm morphologies were observed at high flow velocity and fluid shear locations whereas large clump-like, multilayered biofilm structures were produced at low flow velocity and fluid shear locations. Upon dispersin B treatment, most of the biofilm was detached from the microchannel surface. However, a trace amount of bacterial cells could not be removed from corner locations most likely due to the insufficient wall shear stress of the fluid at these locations. Dispersin B or rifampicin treatment was effective in delaying the dispersal behavior of bacterial cells, but could not completely remove the biofilm. Combined dynamic delivery of dispersin B and rifampicin was found to be effective for complete removal of the S. epidermidis biofilm.
Lee
Joung-Hyun
JH
New Jersey Center for MicroChemical Systems, Department of Chemical, Biomedical and Materials Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
Kaplan
Jeffrey B
JB
Lee
Woo Y
WY
D000117:Acetylglucosamine / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002021:Buffers; D002364:Caseins / Q000737:chemistry; D003198:Computer Simulation; D004347:Drug Interactions; D004867:Equipment Design / Q000295:instrumentation; D006026:Glycoside Hydrolases / Q000494:pharmacology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010710:Phosphates / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry; D012293:Rifampin / Q000494:pharmacology; D012965:Sodium Chloride / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002021:Buffers; D002364:Caseins; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D011492:Protein Hydrolysates; C113831:poly-N-acetyl glucosamine; C026375:trypticase-soy broth; D012965:Sodium Chloride; D006026:Glycoside Hydrolases; D000117:Acetylglucosamine; D012293:Rifampin