The application of differential display as a gene profiling tool.
Differential display is an effective expression profiling tool which was first introduced in 1992. The original technique is discussed along with modifications that have been described over the last several years. A highly reproducible, semihigh-throughput differential display protocol used in our laboratories is described along with an example of its successful application using pancreatic cancer cells. In addition to the work performed in our laboratories, several examples of successful applications of differential display under a number of scenarios are reviewed. Differential display is one of several expression profiling technologies available and is compared with some of them. The future of differential display remains bright and is as applicable today as it was in 1992.
Chang
Ken Chien-Neng
KC
Osteoporosis Research, Women's Health Research Institute, Wyeth Research, Collegeville, PA, USA.
Komm
Barry
B
Arnold
Nichole Bayer
NB
Korc
Murray
M
D015152:Blotting, Northern; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013879:Thioredoxins / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D013879:Thioredoxins