Typeability of AmpFISTR SGM plus loci in kidney, liver, spleen and pancreas tissue samples incubated in different environments.
OBJECTIVE
The aim of the study was assessment of environmental effect on typeability of AmpFISTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA and gender marker amelogenin.


METHODS
Kidney, liver, spleen and pancreas tissue specimens collected during autopsies of five persons aged 20-30 years were incubated at 21 degrees C and 4 degrees C in different environmental conditions. DNA was extracted by organic method from tissue samples collected in 7-day intervals and subsequently typed using AmpFISTR SGM Plus PCR Amplification Kit and ABI 310.


RESULTS
A fast decrease in typeability rate was seen in all tissue specimens incubated in peat soil and in sand. The specimens immersed in pond water and in salt water were partially typeable in all SGM Plus loci within 126 days. Increased air access and higher temperature during our experiments favoured desiccation and preservation of the material resulting in longer typeability of full SGM Plus.


CONCLUSIONS
Decomposed soft tissues are potential material for DNA typing.
Niemcunowicz-Janica
A
A
Department of Forensic Medicine, Medical University of Białystok, Poland.
Pepiński
W
W
Janica
J R
JR
Skawrońska
M
M
Janica
J
J
Koc-Zórawska
E
E
D000328:Adult; D016172:DNA Fingerprinting / Q000379:methods; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013048:Specimen Handling; D013154:Spleen / Q000378:metabolism*; D020080:Tandem Repeat Sequences; D013696:Temperature; D013997:Time Factors