title: Electrochemical analysis of cell plasma membrane cholesterol at the airway surface of mouse trachea.
abstract: Electrochemical detection of plasma membrane cholesterol at the surface of excised mouse trachea tissue is reported. Cholesterol oxidase is covalently linked to an 11-mercaptoundecanoic acid submonolayer on the platinum electrode surface. The cholesterol oxidase-modified electrodes show steady-state responses for cholesterol in solution at physiological temperatures. Experiments for direct contact between the cholesterol oxidase-modified electrode and the surface of excised trachea tissue at 37 degrees C indicate steady-state responses that are largely independent of the position of contact on the tissue surface. Tissue samples are mounted on a quartz crystal microbalance electrode to gauge contact force between the electrode and the tissue surface, and the steady-state electrode response for tissue cholesterol is shown to be largely independent of the contact force. Trachea tissue excised from a mouse model of cystic fibrosis, which is known to exhibit evaluated cholesterol in airway cells, shows an electrode response that is approximately 40% larger than the response observed at wild-type mouse trachea tissue.
authors.affiliation: Department of Chemistry, Case Western Reserve University and Rainbow Babies and Children's Hospital, Cleveland, OH 44106, USA.
authors.forename: Dechen
authors.initials: D
authors.lastname: Jiang
authors.forename: Danjun
authors.initials: D
authors.lastname: Fang
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Kelley
authors.forename: James D
authors.initials: JD
authors.lastname: Burgess
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis* / Q000378:metabolism; D002789:Cholesterol Oxidase / Q000378:metabolism; D003550:Cystic Fibrosis / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004563:Electrochemistry; D005227:Fatty Acids / Q000737:chemistry; D051379:Mice; D008839:Microelectrodes; D010984:Platinum / Q000737:chemistry; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014132:Trachea / Q000378:metabolism* / Q000473:pathology
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D013438:Sulfhydryl Compounds; D010984:Platinum; D002784:Cholesterol; D002789:Cholesterol Oxidase