title: Relationship and significance between anti-beta2-glycoprotein I antibodies and platelet activation state in patients with ulcerative colitis.
abstract: OBJECTIVE
  To study the relationship between anti-beta2-glycoprotein I (abeta2GPI) antibodies and platelet activation state in patients with ulcerative colitis (UC) and its significance.
  
  
  METHODS
  Peripheral blood samples were collected from 56 UC patients (34 males and 22 females, aged 43.5 years, range 21-66 years), including 36 at active stage and 20 at remission stage, and 25 sex-and age-matched controls. The level of abeta2GPI was measured by ELISA. The platelet activation markers, platelet activation complex-I (PAC-I) and P-selectin (CD62P) were detected by flow cytometry.
  
  
  RESULTS
  The A value for IgG abeta2GPI in the active UC group was 0.61 +/- 0.13, significantly higher than that in the remittent UC and control groups (0.50 +/- 0.13 and 0.22 +/- 0.14, P < 0.01). There was a significant difference between the two groups (P < 0.01). The A value for IgM abeta2GPI in the active and remittent UC groups was 0.43 +/- 0.13 and 0.38 +/- 0.12, significantly higher than that in the control group (0.20 +/- 0.12, P < 0.01). However, there was no significant difference between the two groups (P > 0.05). The PAC-I positive rate for the active and remittent UC groups was 30.6% +/- 7.6% and 19.6% +/- 7.8% respectively, significantly higher than that for the control group (6.3% +/- 1.7%, P < 0.01). There was a significant difference between the two groups (P < 0.01). The CD62P positive rate for the active and remittent UC groups was 45.0% +/- 8.8% and 31.9% +/- 7.8% respectively, significantly higher than that for the control group (9.2% +/- 2.7%, P < 0.01). There was a significant difference between the two groups (P < 0.01). In the active UC group, the more severe the state of illness was, the higher the A value for IgG abeta2GPI was, and the positive rate for PAC-I and CD62P was positively correlated with the state of illness (Fabeta2GPI = 3.679, P < 0.05; FPAC-I (%) = 5.346, P < 0.01; and FCD62P (%) = 5. 418, P < 0.01). Meanwhile, in the same state of illness, the A value for IgG abeta2GPI was positively correlated to the positive rates for PAC-I and CD62P.
  
  
  CONCLUSIONS
  abeta2GPI level, platelet activation state and their relationship of them are closely correlated with the pathogenesis and development of UC.
authors.affiliation: Department of Gastroentology, The First Affiliated Hospital, Jilin University, 71 Xinmin Street, Changchun 130021, Jilin Province, China.
authors.forename: Yan-Hang
authors.initials: YH
authors.lastname: Gao
authors.forename: Pu-Jun
authors.initials: PJ
authors.lastname: Gao
authors.forename: Chun-Guang
authors.initials: CG
authors.lastname: Wang
authors.forename: Xiao-Cong
authors.initials: XC
authors.lastname: Wang
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Piao
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D003093:Colitis, Ulcerative / Q000097:blood* / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015539:Platelet Activation / Q000276:immunology*; D053482:beta 2-Glycoprotein I / Q000037:antagonists & inhibitors*
chemical_list: D001323:Autoantibodies; D053482:beta 2-Glycoprotein I