Hepatic metabolism of contaminants in the terapontid fish, yellowtail trumpeter (Amniataba caudavittata Richardson).
The yellowtail trumpeter (Amniataba caudavittata) is an estuarine-dependent omnivorous fish found in the Swan-Canning Estuary, Western Australia. Thirty five fish were injected with either the polycyclic aromatic hydrocarbon, benzo[a]pyrene (B[a]P), the synthetic flavenoid beta-naphthoflavone (BNF), or used as controls. The fish were then sampled at 3 and 7 days postinjection. Induction of the enzyme ethoxyresorufin O-deethylase (EROD) activity was nonsignificant while ethoxycoumarin O-deethylase (ECOD) activity induction differed amongst treatments. A high interindividual variability in the EROD activity was observed. The measurement of sorbitol dehydrogenase in the serum (s-SDH) was elevated (BNF 2.2 times and B[a]P 3.2 times the control fish) demonstrating that liver cell damage had occurred. Increases in biliary metabolites of both B[a]P-type and pyrene-type (19 times and 3.4 times the controls respectively) indicated that detoxification of pyrene-type compounds had taken place. Fish of the Terapontidae family, such as the yellowtail trumpeter, were found to be suitable for biomonitoring the health of the Swan-Canning Estuary. A combination of ECOD activity, s-SDH, and the measurement of biliary metabolites represents a suitable suite of biomarkers for environmental monitoring of the sublethal effects of PAH pollution in these fish.
Webb
Diane
D
Department of Environmental Biology, Curtin University of Technology, Bentley, WA, Australia. d.webb@curtin.edu.au
Gagnon
Marthe Monique
MM
Rose
Tom
T
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity; D001646:Bile / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D005399:Fishes / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D011084:Polycyclic Aromatic Hydrocarbons / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity; D045483:Rivers; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity; D014904:Western Australia; D019324:beta-Naphthoflavone / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity
D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D019324:beta-Naphthoflavone; D007064:L-Iditol 2-Dehydrogenase; D015660:7-Alkoxycoumarin O-Dealkylase; D019363:Cytochrome P-450 CYP1A1