title: Barrier function and cytologic features of the ocular surface epithelium after autologous cultivated oral mucosal epithelial transplantation.
abstract: OBJECTIVE
  To determine the barrier function and cytologic features of ocular surface epithelium after autologous cultivated oral mucosal epithelial transplantation in a prospective observational study.
  
  
  METHODS
  The status of the epithelium in 4 eyes with limbal stem cell deficiency was studied preoperatively and postoperatively. We used an impression method to determine the cytologic features and anterior fluorophotometry to determine barrier function.
  
  
  RESULTS
  Impression cytology showed nonkeratinized, squamous, polygonal, cohesive cells with a low nuclear to cytoplasmic cell ratio and no goblet cells, corresponding to cultivated oral mucosal epithelium, at up to 16 months after surgery. In some cases, the epithelium displayed a mixture of oral mucosal and conjunctival epithelium, especially in cases with a longer postoperative period. Central epithelial permeability remained persistently high throughout the follow-up period, regardless of the epithelial phenotype.
  
  
  CONCLUSIONS
  Cultivated oral mucosal epithelial cells were observed to survive for more than 1 year after transplantation, with gradual replacement by conjunctival epithelium in some cases. Decreased barrier function of the transplanted epithelium may have prognostic implications, suggesting the presence of oral mucosal epithelium long after surgery.
authors.affiliation: Department of Ophthalmology, Tokyo Dental College, Ichikawa, Chiba, Japan. satake@eyebank.or.jp
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Satake
authors.forename: Murat
authors.initials: M
authors.lastname: Dogru
authors.forename: Gen-Yuki
authors.initials: GY
authors.lastname: Yamane
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tsubota
authors.forename: Jun
authors.initials: J
authors.lastname: Shimazaki
mesh_terms: D000328:Adult; D000368:Aged; D001692:Biological Transport / Q000502:physiology; D002463:Cell Membrane Permeability / Q000502:physiology; D002470:Cell Survival; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D003316:Corneal Diseases / Q000378:metabolism / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation*; D019573:Epithelium, Corneal / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D019793:Fluorescein / Q000378:metabolism; D015590:Fluorophotometry; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D016850:Limbus Corneae / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology*; D014182:Transplantation, Autologous
chemical_list: D019793:Fluorescein