Ciz, a transcription factor with a nucleocytoplasmic shuttling activity, interacts with C-propeptides of type I collagen.
Ciz is a zinc finger transcription factor with nucleocytoplasmic shuttling activity. Ciz-deficient mice show high bone mass phenotype. As a first step to address how Ciz suppresses bone formation, we examined the binding partners of Ciz based on a yeast two-hybrid screening. While Ciz is an intracellular protein, 47% of the positive clones were genes encoding extracellular matrix proteins, including Col1a1, Col1a2, Fbln2, and Rpsa. In vitro coimmunoprecipitation experiments using in vitro translated proteins revealed direct binding of Ciz-DeltaZF (zinc finger) to C-propeptides of Col1a1 and Col1a2. In vivo association of the transfected Ciz and C-propeptide of Col1a1 was observed in COS-7 cells based on immunoprecipitation. In terms of intracellular localization, overexpressed C-propeptides of Col1 and Ciz were co-localized in nuclei. These results revealed that Ciz interacts with C-propeptides of type I collagen and this association takes place in nuclei.
Hayata
Tadayoshi
T
Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 3-10 Kanda-Surugadai 2-Chome, Chiyoda-ku, Tokyo 101-0062, Japan.
Nakamoto
Tetsuya
T
Ezura
Yoichi
Y
Noda
Masaki
M
D016475:3T3 Cells; D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D024042:Collagen Type I / Q000378:metabolism*; D051379:Mice; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*; D011485:Protein Binding; D011486:Protein C / Q000378:metabolism*; D025941:Protein Interaction Mapping; D011498:Protein Precursors / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
D024042:Collagen Type I; D034521:Nuclear Matrix-Associated Proteins; D011486:Protein C; D011498:Protein Precursors; D014157:Transcription Factors; C499081:Zfp384 protein, mouse; C054836:protein C propeptide