title: Interactions of elongation factor EF-P with the Escherichia coli ribosome.
abstract: EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site.
authors.affiliation: University of Toronto, C.H. Best Institute, Canada. hiro.dr.aoki@utoronto.ca
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Aoki
authors.forename: John
authors.initials: J
authors.lastname: Xu
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
authors.forename: John G
authors.initials: JG
authors.lastname: Chosay
authors.forename: Ashkan
authors.initials: A
authors.lastname: Golshani
authors.forename: M Clelia
authors.initials: MC
authors.lastname: Ganoza
mesh_terms: D001665:Binding Sites; D004926:Escherichia coli / Q000378:metabolism*; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D010445:Peptide Elongation Factors / Q000378:metabolism*; D011485:Protein Binding; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D010445:Peptide Elongation Factors; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C020746:factor EF-P