Fluorescent nanoparticle for bacteria and DNA detection.
Using bioconjugated dye-doped silica nanoparticles (NPs), we have developed a bioassay for the accurate determination of a single bacterial cell within 20 minutes without any signal amplification or sample enrichment. The antibody-conjugated NPs can specifically and quantitatively detect bacteria, such as Escherichia coli O157:H7 from beef through antibody-antigen recognition. Dye-doped silica NPs have also been successfully used for DNA detection at sub-fentomolar concentrations. Our results demonstrate the potential of dye-doped silica NPs for broad applications in practical biotechnological and medical applications in various biodetection systems. The ultimate goal of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents.
Zhao
Wenjun
W
Department of Chemistry, Center for Research at Bio/Nano Interface, Shands Cancer Center and UF Genetics Institute, University of Florida, Gainesville 32611-7200, USA.
Wang
Lin
L
Tan
Weihong
W
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001681:Biological Assay; D015374:Biosensing Techniques; D015169:Colony Count, Microbial; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D019453:Escherichia coli O157 / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D005506:Food Contamination; D005516:Food Microbiology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry
D005456:Fluorescent Dyes; D004247:DNA