Label-free detection of DNA hybridization at a liquid|liquid interface.
A novel electrochemical approach for label-free detection of DNA primary sequence has been proposed. The flow of nonelectroactive ions across a liquid|liquid interface was used as an electrochemical probe for detection of DNA hybridization. Disposable graphite screen-printed electrodes shielded with a thin layer of inert polymer plasticized with water-immiscible polar organic solvent were modified by probe oligonucleotide and used as a DNA sensor. The specific DNA coupling has been detected with impedance spectroscopy by decrease of ion-transfer resistance. The detection limit was of 10-8 M of target oligonucleotide. The reported sensor was suitable for discrimination of a single mismatch oligonucleotide from the full complementary one. The reported DNA sensor was advantageous over known physicochemical approaches, providing the most significant changes in the measured parameters.
Vagin
Mikhail Yu
MY
Faculty of Chemistry, M.V. Lomonosov Moscow State University, Lenin's Hills, Moscow, Russia. MYV@enzyme.chem.msu.ru
Trashin
Stanislav A
SA
Karyakin
Arkady A
AA
Mascini
Marco
M
D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D006108:Graphite / Q000737:chemistry; D008839:Microelectrodes; D009693:Nucleic Acid Hybridization / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis; D009930:Organic Chemicals / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D012997:Solvents / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D009841:Oligonucleotides; D009930:Organic Chemicals; D011108:Polymers; D012997:Solvents; D014867:Water; D006108:Graphite; D004247:DNA