Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana.
Flagellin, a component of the flagellar filament of Pseudomonas syringae pv. tabaci 6605 (Pta), induces hypersensitive reaction in its non-host Arabidopsis thaliana. We identified the WRKY41 gene, which belongs to a multigene family encoding WRKY plant-specific transcription factors, as one of the flagellin-inducible genes in A. thaliana. Expression of WRKY41 is induced by inoculation with the incompatible pathogen P. syringae pv. tomato DC3000 (Pto) possessing AvrRpt2 and the non-host pathogens Pta within 6-h after inoculation, but not by inoculation with the compatible Pto. Expression of WRKY41 was also induced by inoculation of A. thaliana with an hrp-type three secretion system (T3SS)-defective mutant of Pto, indicating that effectors produced by T3SS in the Pto wild-type suppress the activation of WRKY41. Arabidopsis overexpressing WRKY41 showed enhanced resistance to the Pto wild-type but increased susceptibility to Erwinia carotovora EC1. WRKY41-overexpressing Arabidopsis constitutively expresses the PR5 gene, but suppresses the methyl jasmonate-induced PDF1.2 gene expression. These results demonstrate that WRKY41 may be a key regulator in the cross talk of salicylic acid and jasmonic acid pathways.
Higashi
Kuniaki
K
Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama, 700-8530, Japan.
Ishiga
Yasuhiro
Y
Inagaki
Yoshishige
Y
Toyoda
Kazuhiro
K
Shiraishi
Tomonori
T
Ichinose
Yuki
Y
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005408:Flagellin / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D017343:Genes, Plant; D016973:Pectobacterium carotovorum / Q000472:pathogenicity; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D044224:Pseudomonas syringae / Q000472:pathogenicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D017931:DNA Primers; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C496136:WRKY53 protein, Arabidopsis; D005408:Flagellin