Outer membrane modifications of Pseudomonas fluorescens MF37 in response to hyperosmolarity.
The effect of hyperosmotic condition on the outer membrane protein (Omp) composition of Pseudomonas fluorescens was investigated by proteomic analyses. The abundances of 12 proteins, including porins, lipoproteins, and the flagella subunit FliC, were modified. This was at least partly explained by altered gene expression, as shown by mRNA level study. In agreement with Omp changes, hyperosmotic condition resulted in vesicle formation and modifications of mobility and antibiotic susceptibility.
Guyard-Nicodème
Muriel
M
Laboratoire de Microbiologie du Froid, UPRES EA 2123, Université de Rouen, Evreux, France.
Bazire
Alexis
A
Hémery
Gaëlle
G
Meylheuc
Thierry
T
Mollé
Daniel
D
Orange
Nicole
N
Fito-Boncompte
Laurène
L
Feuilloley
Marc
M
Haras
Dominique
D
Dufour
Alain
A
Chevalier
Sylvie
S
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001425:Bacterial Outer Membrane Proteins; D012333:RNA, Messenger