Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation.
Protein phosphatase-2A (PP-2A) interacts with several regulators of cell death pathways and is therefore a potential component of signaling pathways linking global cerebral ischemia to cell death. Using a novel procedure to quantify PP-2A activity, we find that cardiac arrest with resuscitation and reperfusion leads to activation of PP-2A by 1.6-fold in pig brain extract and by 3.4-fold after partial purification of PP-2A. This is the first demonstration of PP-2A activation in a clinically relevant model of transient global cerebral ischemia. These results suggest that inhibition of PP-2A activity may be neuroprotective in global cerebral ischemia.
Zhang
Tao T
TT
Institute for Neuronal Cell Signaling, Department of Anesthesiology, Weill Medical College of Cornell University, New York, NY 10021, USA.
Platholi
Jimcy
J
Heerdt
Paul M
PM
Hemmings
Hugh C
HC
Tung
H Y L
HY
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001921:Brain / Q000201:enzymology*; D002545:Brain Ischemia / Q000201:enzymology; D016887:Cardiopulmonary Resuscitation; D016923:Cell Death / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006323:Heart Arrest / Q000201:enzymology*; D009336:Necrosis / Q000473:pathology; D054648:Protein Phosphatase 2 / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D015427:Reperfusion Injury / Q000201:enzymology; D013552:Swine
D004791:Enzyme Inhibitors; D054648:Protein Phosphatase 2