Poly-N-acetylglucosamine and poly(glycerol phosphate) teichoic acid identification from staphylococcal biofilm extracts using excitation sculptured TOCSY NMR.
We report the successful application of selective excitation sculptured TOCSY NMR (SXS-TOCSY) to identify individual solution components from a heterogeneous system using selectively acquired (1)H NMR spin system patterns. SXS-TOCSY application is illustrated by detection of the simultaneous presence of poly-beta-(1,6)-N-acetylglucosamine (PNAG) and poly(glycerol phosphate) teichoic acid (TA) carbohydrate polymer components in crude biofilm extracts from Staphylococcus epidermidis without the need for further sample purification and component separation. Biofilms are implicated in the barriers for resistance of microbes toward antibiotics and immune responses, therefore efficient rapid detection and quantification of key components are important to assist in the design of a clinical infection response.
Wagstaff
Jane L
JL
Protein Science Group, Department of Biosciences, University of Kent, Canterbury, Kent, UKCT2 7NJ.
Sadovskaya
Irina
I
Vinogradov
Evgeny
E
Jabbouri
Saïd
S
Howard
Mark J
MJ
D018441:Biofilms; D009682:Magnetic Resonance Spectroscopy; D013210:Staphylococcus / Q000737:chemistry* / Q000502:physiology*; D013682:Teichoic Acids / Q000737:chemistry*; D047071:beta-Glucans / Q000737:chemistry*
D013682:Teichoic Acids; D047071:beta-Glucans; C505465:poly-N-acetyl-1-6-glucosamine