title: FabG mediates polyhydroxyalkanoate production from both related and nonrelated carbon sources in recombinant Escherichia coli LS5218.
abstract: Polyhydroxyalkanoates (PHAs) composed of a mixture of short-chain-length-medium-chain-length (SCL-MCL) hydroxyacyl monomers are biologically produced polyesters that have properties ranging from thermoplastic to elastomeric, dependent on the molar ratio of SCL to MCL monomers incorporated into the copolymer. Because of the potential wide range of properties and applications for SCL-MCL PHA copolymers, it is important to develop and characterize novel metabolic pathways for SCL-MCL PHA production. The current study shows that coexpression of fabG genes from either E. coli or Pseudomonas sp. 61-3 with fabH(F87T) and PHA synthase genes enhances the production of SCL-MCL PHA copolymer from both related and nonrelated carbon sources in Escherichia coli LS5218, indicating the flexibility of FabG as a monomer-supplying enzyme for biological PHA production.
authors.affiliation: Department of Chemistry, State University of New York-College of Environmental Science and Forestry, Syracuse, NY 13210, USA. ctnomura@esf.edu
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Nomura
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Tanaka
authors.forename: Tenai E
authors.initials: TE
authors.lastname: Eguen
authors.forename: Alexandria S
authors.initials: AS
authors.lastname: Appah
authors.forename: Ken'ichiro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Seiichi
authors.initials: S
authors.lastname: Taguchi
authors.forename: C Leo
authors.initials: CL
authors.lastname: Ortiz
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Doi
mesh_terms: D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010957:Plasmids; D054813:Polyhydroxyalkanoates / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D054813:Polyhydroxyalkanoates; D002244:Carbon; D000429:Alcohol Oxidoreductases; C023744:acetoacetyl-CoA reductase