Chemical fingerprinting of Hoodia species and related genera: chemical analysis of oxypregnane glycosides using high-performance liquid chromatography with UV detection in Hoodia gordonii.
Hoodia gordonii, family Asclepiadaceae, is a succulent plant and is traditionally used in southern Africa for its appetite-suppressant properties. A high-performance liquid chromatographic (HPLC) method with UV detection for analysis of 11 oxypregnane glycosides from H. gordonii has been developed. The simultaneous analysis of 11 oxypregnane glycosides was achieved with a Phenomenex (Torrance, CA) reversed-phase C18 column using gradient mobile phase of water and acetonitrile, both containing 0.025% trifluoroacetic acid. The developed method was applied to the identification of oxypregnane glycosides in 3 different species of Hoodia and 23 related genera. The HPLC profiles of various plant samples were compared for the presence of oxypregnane glycosides.
Avula
Bharathi
B
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA. bavula@olemiss.edu
Wang
Yan-Hong
YH
Pawar
Rahul S
RS
Shukla
Yatin J
YJ
Khan
Ikhlas A
IA
D029062:Apocynaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000032:analysis; D007202:Indicators and Reagents; D011278:Pregnanes / Q000032:analysis; D012015:Reference Standards; D012996:Solutions; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
D006027:Glycosides; D007202:Indicators and Reagents; D011278:Pregnanes; D012996:Solutions