An in vitro evaluation of the growth of human periodontal ligament fibroblasts after exposure to a methacrylate-based endodontic sealer.
The cytotoxicity of Epiphany root canal sealer at various concentrations from 25-800 microg/mL on human periodontal ligament (HPDL) fibroblasts was evaluated at 1, 3, and 7 days. Controls included untreated cells and cells treated with the vehicle for Epiphany suspension. Fibroblast viability was assessed by 2 methods, crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates. Significant cytotoxicity against HPDL fibroblast growth by Epiphany was both time- and concentration-dependent. On day 1, 800 microg/mL, the highest concentration of Epiphany, showed significant cytotoxicity (P < or = .001). By day 7, all concentrations greater than 25 microg/mL showed significant (P < or = .05) loss of viability. This study demonstrated increased Epiphany cytotoxicity with an increase in concentration or exposure time.
Heitman
Erich P
EP
U.S. Army Endodontic Residency Program, Fort Gordon, Georgia 30905, USA. erich.heitman@us.army.mil
Joyce
Anthony P
AP
McPherson
James C
JC
Roberts
Steven
S
Chuang
Augustine
A
D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005840:Gentian Violet; D006801:Humans; D008689:Methacrylates / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
D004396:Coloring Agents; D008689:Methacrylates; D012387:Root Canal Filling Materials; C486893:epiphany sealer; D005840:Gentian Violet