title: Oxidant-induced cell-cycle delay in Saccharomyces cerevisiae: the involvement of the SWI6 transcription factor.
abstract: Cells treated with low doses of linoleic acid hydroperoxide (LoaOOH) exhibit a cell-cycle delay that may provide a mechanism to overcome oxidative stress. Strains sensitive to LoaOOH from the genome-wide deletion collection were screened to identify deletants in which the cell-cycle delay phenotype was reduced. Forty-seven deletants were identified that were unable to mount the normal delay response, implicating the product of the deleted gene in the oxidant-mediated cell-cycle delay of the wild-type. Of these genes, SWI6 was of particular interest due to its role in cell-cycle progression through Start. The swi6 deletant strain was delayed on entry into the cell cycle in the absence of an oxidant, and oxidant addition caused no further delay. Transforming the swi6 deletant with SWI6 on a plasmid restored the G1 arrest in response to LoaOOH, indicating that Swi6p is involved in oxidant sensing leading to cell division delay. Micro-array studies identified genes whose expression in response to LoaOOH depended on SWI6. The screening identified 77 genes that were upregulated in the wild-type strain and concurrently downregulated in the swi6 deletant treated with LoaOOH. These data show that functions such as heat shock response, and glucose transport are involved in the response.
authors.affiliation: Ramaciotti Centre for Gene Function Analysis and School of Biotechnology and Biomolecular Sciences, UNSW, NSW, Australia.
authors.forename: Chii Shyang
authors.initials: CS
authors.lastname: Fong
authors.forename: Mark D
authors.initials: MD
authors.lastname: Temple
authors.forename: Nazif
authors.initials: N
authors.lastname: Alic
authors.forename: Joyce
authors.initials: J
authors.lastname: Chiu
authors.forename: Moritz
authors.initials: M
authors.lastname: Durchdewald
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Thorpe
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Higgins
authors.forename: Ian W
authors.initials: IW
authors.lastname: Dawes
mesh_terms: D002453:Cell Cycle / Q000187:drug effects*; D006019:Glycolysis; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D008041:Linoleic Acids; D008054:Lipid Peroxides; C075111:SWI6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; C008285:linoleic acid hydroperoxide