A mouse model of intracerebral hemorrhage using autologous blood infusion.
The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.
Rynkowski
Michal A
MA
Department of Neurological Surgery, Columbia University, 710 West 168th Street, New York, New York 10032, USA.
Kim
Grace H
GH
Komotar
Ricardo J
RJ
Otten
Marc L
ML
Ducruet
Andrew F
AF
Zacharia
Brad E
BE
Kellner
Christopher P
CP
Hahn
David K
DK
Merkow
Maxwell B
MB
Garrett
Matthew C
MC
Starke
Robert M
RM
Cho
Byung-Moon
BM
Sosunov
Sergei A
SA
Connolly
E Sander
ES
D000818:Animals; D001479:Basal Ganglia; D001804:Blood Transfusion, Autologous; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000209:etiology*; D004195:Disease Models, Animal; D007260:Infusion Pumps; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011182:Postoperative Care