Influence of cancer-related gene polymorphisms on clinicopathological features in colorectal cancer.
OBJECTIVE
Single nucleotide polymorphisms (SNP) are shown to be related with cancer incidence. It has been reported that CCND1, p21(cip1)DCC, MTHFR, and EXO1 are related with the risk of malignant neoplasm, but few studies have mentioned the prognosis of the patients. We investigated the SNP of patients and related this to clinicopathological features, including survival rate.


METHODS
DNA from the tissues of primary colorectal cancer was obtained from surgical resections of 114 patients (68 males and 46 females, 29-83 years). The CCND1 polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and those of other genes were investigated by the TaqMan method. The polymorphisms obtained were statistically analyzed for the relationship with clinicopathological features.


RESULTS
The CG + GG allele was more invasive than the CC allele in histological tumor depth in the DCC codon 201 (P = 0.0086). The 677TT allele in MTHFR had a larger tumor size than the 677CC allele (P = 0.028). In EXO1 P757L polymorphism, patients with the TT allele had a statistically reduced survival rate compared with the other alleles. In CCND1 polymorphisms, we found no statistical significance in clinicopathological features.


CONCLUSIONS
From these preliminary data, these polymorphisms would be candidates predicting the clinicopathological features of colorectal cancer, but further more systematic gene analyses are warranted.
Yoshiya
Gen
G
Department of Gastroenterology and Hematology, Hirosaki University Graduate School of Medicine, Aomori, Japan.
Takahata
Takenori
T
Hanada
Naoyuki
N
Suzuki
Kazuhiro
K
Ishiguro
Atsushi
A
Saito
Masato
M
Sasaki
Mutsuo
M
Fukuda
Shinsaku
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D056741:Cyclin D; D016213:Cyclins / Q000235:genetics; D005260:Female; D016163:Genes, DCC; D005838:Genotype; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
D056741:Cyclin D; D016213:Cyclins; D042965:Methylenetetrahydrofolate Reductase (NADPH2)