Alterations of FHIT and P53 genes in keratocystic odontogenic tumor, dentigerous and radicular cyst.
BACKGROUND
The purpose of this study was to determine fragile histidine triad (FHIT) and p53 protein expression, and to analyze FHIT and p53 gene status in keratocystic odontogenic tumor (KOT), dentigerous cysts (DC) and radicular cysts (RC).


METHODS
The methods used were immunohistochemistry and molecular genetic methods including loss of heterozygosity (LOH) and gene sequencing.


RESULTS
FHIT protein expression was different among groups. Aberrant expression was the highest in KOT, then in RC and DC. p53 protein expression was different among groups. LOH in paraffin-embedded specimens was detected in 22.6% and 12.9% for FHIT and p53 respectively. Mutation of p53 gene at codon 237 was observed in only two specimens (one KOT and one DC). Of the six frozen specimens, three exhibited FHIT gene LOH (two RC and one KOT). KOT showed loss of exons 6-7 at FHIT locus and mutation at codon 237 at p53 locus, but this could be a chance result.


CONCLUSIONS
Aberrations of FHIT and p53 genes/proteins could be considered markers responsible for the development of odontogenic lesions.
Malcić
Ana
A
Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia. amalcic@inet.hr
Jukić
Silvana
S
Anić
Ivica
I
Pavelić
Bozidar
B
Kapitanović
Sanja
S
Kruslin
Bozo
B
Pavelić
Kresimir
K
D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D049109:Cell Proliferation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D003803:Dentigerous Cyst / Q000235:genetics* / Q000378:metabolism; D005260:Female; D016147:Genes, Tumor Suppressor; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007570:Jaw Cysts / Q000235:genetics* / Q000378:metabolism; D007573:Jaw Neoplasms / Q000235:genetics* / Q000378:metabolism; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D009808:Odontogenic Tumors / Q000235:genetics* / Q000378:metabolism; D011842:Radicular Cyst / Q000235:genetics* / Q000378:metabolism; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase