Determination of imipenem and rifampicin in mouse plasma by high performance liquid chromatography-diode array detection.
A high performance liquid chromatographic (HPLC) method for the determination of imipenem and rifampicin was developed and validated. The method involves plasma deproteinisation with methanol, gradient elution on a RP-18 column and diode array detection. Separation was carried out in 8 min using a mobile phase composed of methanol and 0.2M borate buffer (pH 7.2). Imipenem and rifampicin were detected at 300 nm and 255 nm, respectively. A linear response was observed at plasma levels ranged between 0.3 and 30 microgmL(-1) for imipenem and 1.5 and 20 microgmL(-1) for rifampicin. The detection limits were 0.07 microgmL(-1) and 0.47 microgmL(-1) for imipenem and rifampicin, respectively. The method was applied to the determination of both compounds in mouse plasma samples.
Fernández-Torres
R
R
Department of Analytical Chemistry, Faculty of Chemistry, 41012 Seville, Spain.
Bello-López
M A
MA
Callejón-Mochón
M
M
Jiménez-Sánchez
J C
JC
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D015378:Imipenem / Q000097:blood* / Q000737:chemistry; D054023:Lasers, Semiconductor; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D012293:Rifampin / Q000097:blood* / Q000737:chemistry
D015378:Imipenem; D012293:Rifampin