Comparison of ethinylestradiol and nonylphenol effects on reproduction of Chinese rare minnows (Gobiocypris rarus).
Breeding pairs of rare minnows were exposed to 5, 10, 20 microg/L 4-nonylphenol (NP) or 4 ng/L 17alpha-ethinylestradiol (EE(2)) for 21 days. Among reproductive parameters investigated, fecundity, fertility, and laying interval were significantly affected by 4 ng/L EE(2), but were not affected at any exposure level of NP. These effects coincided with male feminization, ova-testis, increased plasma VTG in both males and females as well as the accumulation of eosinophilic material in kidneys and livers. Histopathologic evaluation revealed severe kidney lesions in both EE(2) and NP-exposed fish. The bioassay succeeded in demonstrating that EE(2) was several orders of magnitude more potent than the industrial chemical NP. The rare minnow was more sensitive to EE(2) than medaka, but comparable to zebrafish in terms of sensitivity. The results of the present investigation indicated that a short-term reproductive bioassay is feasible using the Chinese rare minnow as the test species.
Zha
Jinmiao
J
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, PR China.
Sun
Liwei
L
Spear
Philip A
PA
Wang
Zijian
Z
D000818:Animals; D003530:Cyprinidae / Q000502:physiology*; D012734:Disorders of Sex Development / Q000139:chemically induced; D004305:Dose-Response Relationship, Drug; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D010053:Ovary / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D012098:Reproduction / Q000187:drug effects*; D012671:Seminiferous Tubules / Q000187:drug effects; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000633:toxicity
D010636:Phenols; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol; C025256:nonylphenol