title: Analysis of human oral mucosa ex vivo for fatty acid compositions using Fourier-transform infrared spectroscopy.
abstract: The purpose of this research was to develop an infrared spectroscopic technique (Fourier transform infrared spectroscopy with attenuated total reflectance system; FTIR-ATR) for non-invasive measurement of saturated and unsaturated fatty acid compositions in human oral mucosa obtained from three nationalities; Iranian, Vietnamese, and Indonesian. The histogram patterns of fatty acid compositions for three nationalities suggest that the pattern of unsaturated fatty acids were quite different, although the distribution profiles of fatty acid to lipid ratios in FTIR-ATR has a similar normal pattern with small difference in skewness and mode. The second derivative infrared spectra of the mucosal tissues in the wavenumber regions from 1,600 to 1,760 cm(-1) and 2,800 to 3,050 cm(-1) were analyzed with partial least squares (PLS) multivariate regression analysis method. With this analysis method we compared predicted values with the measured values of ten categorized fatty acid compositions, i.e., a(saturated C17 or lower), b(C16:1 + C17:1), c(C18:0), d(C18:1), e(C18:2), f(saturated C20 or longer), g(C20:3 + C20:4), h(C22:1 + C24:1), i(C22:6), j(gammaC18:3). Almost all fatty acid compositions of oral mucosa were well predicted with differences between predicted and measured values within +/-5% of total, however, errors were relatively larger in minor components such as C22:6 than major components.
authors.affiliation: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan. xyosida@gifu-u.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamashita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
authors.forename: Reza
authors.initials: R
authors.lastname: Ghadimi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hosono
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Kiyonori
authors.initials: K
authors.lastname: Kuriki
authors.forename: Sadao
authors.initials: S
authors.lastname: Suzuki
authors.forename: Shinkan
authors.initials: S
authors.lastname: Tokudome
mesh_terms: D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000737:chemistry; D006801:Humans; D009061:Mouth Mucosa / Q000737:chemistry* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated