Generation and transplantation of dopaminergic neurons derived from embryonic stem cells.
Human embryonic stem cells (hESCs) can be propagated as undifferentiated cells over multiple passages and thus offer a renewable source of a wide range of cell types for use in research and cell-based therapies. Because of the important roles of dopaminergic (DA) neurons in modulating motor control, and in particular that the selective degeneration of DA neurons in midbrain cause the neurodegenerative disorder Parkinson's disease (PD), there has been significant interest in using hESC-derived DA neurons as a source of cells for drug screening, discovery and transplant purposes. Different strategies have evolved to generate appropriately mature DA neurons that have been purified and/or enriched by one of several selection techniques. In this manuscript we discuss recent results, review the remarkable progress that has been made in the field, and compare these results with those obtained from mouse ESCs (mESCs).
Khanna
Aparna
A
Embryonic Stem Cell Group, Reliance Life Sciences Pvt Ltd., Dhirubhai Ambani Life Sciences Centre, R-282, TTC Industrial Area of MIDC, Thane Belapur Road, Rabale, Navi Mumbai 400 701, India.
Swistowska
Anna Maria
AM
Rao
Mahendra S
MS
Zeng
Xianmin
X
D000818:Animals; D002454:Cell Differentiation; D064987:Cell- and Tissue-Based Therapy; D004195:Disease Models, Animal; D004298:Dopamine / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D009474:Neurons / Q000166:cytology* / Q000637:transplantation*
D004298:Dopamine