Mechanism of hyperbaric oxygen preconditioning in neonatal hypoxia-ischemia rat model.
Hypoxic ischemic (HI) injury in neonates damages brain tissues. We examined the mechanism of hyperbaric oxygen preconditioning (HBO-PC) in neonatal HI rat model. Seven-day-old rat pups were subjected to left common carotid artery ligation and hypoxia (8% oxygen at 37 degrees C) for 90 min. HBO (100% O(2), 2.5 atmospheres absolute for 2.5 h) were administered by placing pups in a chamber 24 h before HI insult. Brain injury was assessed by the survival rate, 2,3,5-triphenyltetrazolium chloride (TTC), Nissl, TUNEL straining and caspase-3,caspase-9 activities after HI. In HBO preconditioned animals, survival rate was increased, infarct ratio was decreased, and the positive stained TUNEL cells were reduced, accompanied by the suppression of caspase-3 and -9 activities. These results indicate that a single HBO-PC appears to provide brain protection against HI insult via inhibition of neuronal apoptosis pathways.
Li
Zhang
Z
Department of Pathology, Weifang Medical College, Shandong, 261042, PR China.
Liu
Wenwu
W
Kang
Zhimin
Z
Lv
Shijun
S
Han
Cuihong
C
Yun
Liu
L
Sun
Xuejun
X
Zhang
John H
JH
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D020520:Brain Infarction / Q000209:etiology / Q000517:prevention & control; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D002452:Cell Count; D016923:Cell Death; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy*; D020287:In Situ Nick-End Labeling / Q000379:methods; D019194:Ischemic Preconditioning / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013778:Tetrazolium Salts
D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D053148:Caspase 3; D053453:Caspase 9