title: Anti-alpha-actinin antibodies cross-react with anti-ssDNA antibodies in active autoimmune hepatitis.
abstract: Recently, we have observed that the association of anti-F-actin autoantibodies (Abs) with anti-alpha-actinin Abs (double reactivity) was correlated with liver disease activity in autoimmune hepatitis type 1 (AIH-1; Guéguen et al., J Clin Immunol 26:495-505, 2006). As anti-alpha-actinin Abs are also associated with anti-single-stranded (ss) DNA in 16 of 50 (32%) AIH-1 patients, compared with 16 of 401 (4.0%) liver disease control patients, we sought to characterize these Abs in AIH-1. When associated, anti-ssDNA Abs cross-react with anti-alpha-actinin Abs, but neither with anti-double-stranded DNA Abs nor with anti-F-actin Abs. Furthermore, anti-alpha-actinin Abs are associated with high-avidity anti-ssDNA Abs in AIH-1. In addition, double reactivity against alpha-actinin and ssDNA was correlated with clinical and histological activity of the disease and untreated AIH-1 patients.
authors.affiliation: Brest University Medical School Hospital, Brest Cedex, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Renaudineau
authors.forename: Geogios N
authors.initials: GN
authors.lastname: Dalekos
authors.forename: Paul
authors.initials: P
authors.lastname: Guéguen
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Zachou
authors.forename: Pierre
authors.initials: P
authors.lastname: Youinou
mesh_terms: D000185:Actinin / Q000276:immunology* / Q000378:metabolism; D000199:Actins / Q000276:immunology / Q000378:metabolism; D000974:Antibodies, Antinuclear / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D015551:Autoimmunity; D003429:Cross Reactions; D004277:DNA, Single-Stranded / Q000276:immunology*; D019693:Hepatitis, Autoimmune / Q000276:immunology*; D006801:Humans
chemical_list: D000199:Actins; D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D004277:DNA, Single-Stranded; D000185:Actinin