Acute responses in muscle mitochondrial and cytosolic enzyme activities during heavy intermittent exercise.
To examine the effects of repetitive bouts of heavy exercise on the maximal activities of enzymes representative of the major metabolic pathways and segments, 13 untrained volunteers [peak aerobic power (Vo(2 peak)) = 44.3 +/- 2.3 ml.kg(-1).min(-1)] cycled at approximately 91% Vo(2 peak) for 6 min once per hour for 16 h. Maximal enzyme activities (V(max), mol.kg(-1).protein.h(-1)) were measured in homogenates from tissue extracted from the vastus lateralis before and after exercise at repetitions 1 (R1), 2 (R2), 9 (R9), and 16 (R16). For the mitochondrial enzymes, exercise resulted in reductions (P < 0.05) in cytochrome-c oxidase (COX, 14.6%), near significant reductions in malate dehydrogenase (4.06%; P = 0.06) and succinic dehydrogenase (4.82%; P = 0.09), near significant increases in beta-hydroxyacyl-CoA dehydrogenase (4.94%; P = 0.08), and no change in citrate synthase (CS, 2.88%; P = 0.37). For the cytosolic enzymes, exercise reduced (P < 0.05) V(max) in hexokinase (Hex, 4.4%), creatine phosphokinase (9.0%), total phosphorylase (13.5%), phosphofructokinase (16.6%), pyruvate kinase (PK, 14.1%) and lactate dehydrogenase (10.7%). Repetition-dependent reductions (P < 0.05) in V(max) were observed for CS (R1, R2 > R16), COX (R1, R2 > R16), Hex (1R, 2R > R16), and PK (R9 > R16). It is concluded that heavy exercise results in transient reductions in a wide range of enzymes involved in different metabolic functions and that in the case of selected enzymes, multiple repetitions of the exercise reduce average V(max).
Green
H J
HJ
Dept. of Kinesiology, Univ. of Waterloo, Waterloo, ON, Canada. green@healthy.uwaterloo.ca
Bombardier
E B
EB
Duhamel
T A
TA
Holloway
G P
GP
Tupling
A R
AR
Ouyang
J
J
D000328:Adult; D001642:Bicycling; D001786:Blood Glucose / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D006003:Glycogen / Q000096:biosynthesis; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D008931:Mitochondria, Muscle / Q000201:enzymology*; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism; D025221:Phosphofructokinases / Q000378:metabolism; D010766:Phosphorylation; D011770:Pyruvate Kinase / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D001786:Blood Glucose; D027501:Monocarboxylic Acid Transporters; D006003:Glycogen; D006903:Hydroxymethylglutaryl CoA Reductases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D025221:Phosphofructokinases; D011770:Pyruvate Kinase; D003402:Creatine Kinase; D000254:Sodium-Potassium-Exchanging ATPase; D007785:Lactose; D002118:Calcium