Prevalence of 4977bp deletion in mitochondrial DNA from patients with chronic kidney disease receiving conservative treatment or hemodialysis in southern Brazil.
BACKGROUND
Damage to mitochondrial DNA (mtDNA) has been described in patients with chronic kidney disease (CKD). The presence of mtDNA 4977bp deletion in many different tissues can serve as a marker of this damage. However, no attempt has been made to detect the presence of mtDNA 4977bp in blood cells of patients with CKD.


METHODS
Polymerase chain reaction techniques (PCR) were used to detect mtDNA 4977bp deletion in blood samples of 94 CKD patients.


RESULTS
The prevalence of 4977bp deletion in mtDNA was 73.1% (38/52) in patients with CKD undergoing hemodialysis, 57.1% (27/42) in patients with CKD receiving conservative treatment, and 27.8% (15/54) in control samples (p < 0.001). Higher prevalence of this mutation was not associated with patient age (p = 0.54) or time on hemodialysis (p = 0.70).


CONCLUSIONS
The higher prevalence of mtDNA 4977bp deletion in patients in this study indicates that the CKD can induce damage to mtDNA in blood cells and could be exacerbated by hemodialysis.
Rossato
Liana Bertolin
LB
Graduate Program in Medical Science, Nephrology and Hemodialysis Service, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. liana_rossato@yahoo.com.br
Nunes
Ane Cláudia Fernandes
AC
Pereira
Maria Luiza Saraiva
ML
de Souza
Carolina Fischinger Moura
CF
Dummer
Claus
C
Milani
Vagner
V
Porsch
Daiana Benck
DB
de Mattos
Cristiane Bastos
CB
Barros
Elvino José Guardão
EJ
D000328:Adult; D001483:Base Sequence; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000628:therapy; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D006435:Renal Dialysis; D017384:Sequence Deletion
D004272:DNA, Mitochondrial