Radioprotection against DNA damage by an extract of Indian green mussel, Perna viridis (L).
This study describes the radioprotective ability of a hydrolysate prepared using an enzyme-acid hydrolysis method from the green mussel Perna viridis in terms of its ability to prevent radiation-induced damage in plasmid DNA, cell death, reactive oxygen species (ROS) formation, and DNA damage in mice lymphocytes. The mussel hydrolysate (MH) present during irradiation showed significant protection from gamma-radiation-induced strand breaks in plasmid DNA as evaluated by gel electrophoresis. Viability studies by trypan blue dye exclusion and MTT assay showed that preincubation of mice splenic lymphocytes with MH protected them from gamma-radiation-mediated killing. Moreover, the presence of MH during irradiation of isolated mice lymphocytes significantly decreased the DNA damage, as measured by comet assay. Measurement of intracellular ROS by dichlorofluorescein fluorescence revealed that the presence of MH effectively reduced the ROS generated in lymphocytes by both chemical method and gamma-irradiation. Prevention of DNA damage both in plasmid and lymphocytes and cell death in lymphocytes appears correlated with reduction of oxidatively generated free radicals. It is concluded that protection against radiation-induced cell death and DNA damage by MH was attributable to reduction of reactive free radical species generated by gamma-radiation.
Kumaran
Sreekumar P
SP
National Institute of Oceanography, Dona Paula, Goa-403 004, India. sreeku21@rediffmail.com
Kutty
Binoj C
BC
Chatterji
Anil
A
Subrayan
Parameswaran P
PP
Mishra
Kaushala Prasad
KP
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D005720:Gamma Rays / Q000009:adverse effects*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D049883:Perna / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*
D005609:Free Radicals; D011837:Radiation-Protective Agents; D014020:Tissue Extracts