title: West Nile virus is neutralized by HOCl-modified human serum albumin that binds to domain III of the viral envelope protein E.
abstract: The outbreaks of West Nile virus (WNV), an emerging flavivirus recently implicated in outbreaks of fatal encephalitis, necessitate the development of effective anti-WNV drugs. In this study, it is demonstrated that human serum albumin is transformed into a WNV antiviral substance by hypochlorite (HOCl) modification. The HOCl-modified albumin efficiently neutralized WNV in vitro (EC50=300 nM) and showed binding to a recombinant protein, representing the domain III of the WNV external envelope E glycoprotein.
authors.affiliation: Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany. markus.vossmann@ge.com
authors.forename: Markus
authors.initials: M
authors.lastname: Vossmann
authors.forename: Martin
authors.initials: M
authors.lastname: Kirst
authors.forename: Diana
authors.initials: D
authors.lastname: Ludolfs
authors.forename: Michael
authors.initials: M
authors.lastname: Schreiber
mesh_terms: D000818:Animals; D001665:Binding Sites; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D017434:Protein Structure, Tertiary; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010948:Viral Plaque Assay; D014774:Virulence / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects; D014902:West Nile virus / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology
chemical_list: D012709:Serum Albumin; D014759:Viral Envelope Proteins; D006997:Hypochlorous Acid