title: Wide varieties of cationic nanoparticles induce defects in supported lipid bilayers.
abstract: Nanoparticles with widely varying physical properties and origins (spherical versus irregular, synthetic versus biological, organic versus inorganic, flexible versus rigid, small versus large) have been previously noted to translocate across the cell plasma membrane. We have employed atomic force microscopy to determine if the physical disruption of lipid membranes, formation of holes and/or thinned regions, is a common mechanism of interaction between these nanoparticles and lipids. It was found that a wide variety of nanoparticles, including a cell penetrating peptide (MSI-78), a protein (TAT), polycationic polymers (PAMAM dendrimers, pentanol-core PAMAM dendrons, polyethyleneimine, and diethylaminoethyl-dextran), and two inorganic particles (Au-NH2, SiO2-NH2), can induce disruption, including the formation of holes, membrane thinning, and/or membrane erosion, in supported lipid bilayers.
authors.affiliation: Department of Chemistry, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, Michigan 48019, USA.
authors.forename: Pascale R
authors.initials: PR
authors.lastname: Leroueil
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Berry
authors.forename: Kristen
authors.initials: K
authors.lastname: Duthie
authors.forename: Gang
authors.initials: G
authors.lastname: Han
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.forename: Daniel Q
authors.initials: DQ
authors.lastname: McNerny
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
authors.forename: Bradford G
authors.initials: BG
authors.lastname: Orr
authors.forename: Mark M Banaszak
authors.initials: MM
authors.lastname: Holl
mesh_terms: D002412:Cations; D003460:Crystallization / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008560:Membrane Fluidity; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D002412:Cations; D008051:Lipid Bilayers; D046911:Macromolecular Substances