title: Potato glycoalkaloids in soil-optimising liquid chromatography-time-of-flight mass spectrometry for quantitative studies.
abstract: Potato glycoalkaloids are produced in high amounts in potato fields during the growth season and losses to soil potentially impact shallow groundwater and via tiles to fresh water ecosystems. A quantitative liquid chromatography-electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS) method for determination and quantification of potato glycoalkaloids and their metabolites in aqueous soil extracts was developed. The LC-ESI-TOF-MS method had linearities up to 2000microg/L for alpha-solanine and alpha-chaconine and up to 760microg/L for solanidine. No matrix effect was observed, and the detection limits found were in the range 2.2-4.7microg/L. The method enabled quantification of the potato glycoalkaloids in environmental samples.
authors.affiliation: Department of Natural Sciences, Faculty of Life Sciences (LIFE), University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark. phj@life.ku.dk
authors.forename: Pia H
authors.initials: PH
authors.lastname: Jensen
authors.forename: René K
authors.initials: RK
authors.lastname: Juhler
authors.forename: Nikoline J
authors.initials: NJ
authors.lastname: Nielsen
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Hansen
authors.forename: Bjarne W
authors.initials: BW
authors.lastname: Strobel
authors.forename: Ole S
authors.initials: OS
authors.lastname: Jacobsen
authors.forename: John
authors.initials: J
authors.lastname: Nielsen
authors.forename: Hans Christian B
authors.initials: HC
authors.lastname: Hansen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012987:Soil / Q000032:analysis*; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000737:chemistry; D012992:Solanine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D012987:Soil; D012991:Solanaceous Alkaloids; C514919:alpha-solanine; D012992:Solanine; C011860:alpha-chaconine