title: Role of Toll-like receptor 4 in lung inflammation following exposure to swine barn air.
abstract: The authors tested a hypothesis that lung inflammation and airway hyperresponsiveness (AHR) induced following barn air exposure are dependent on Toll-like receptor 4 (TLR4) by exposing C3HeB/FeJ (intact TLR4, wild type [WT]) and C3H/HeJ (defective TLR4, mutant) mice either to the barn air (8 hours/day for 1, 5, or 20 days) or ambient air. Both strains of mice, compared to their respective controls, showed increased AHR following 5 exposures but dampened AHR after 20 exposures to show lack of effect of TLR4 on AHR. However, swine barn air induced lung inflammation with recruitment of inflammatory cells and cytokine expression was observed in WT but not in mutant mice. These data show different roles of TLR4 in lung inflammation and AHR in mice exposed to swine barn air.
authors.affiliation: Immunology Research Group, and Department of Veterinary Biomedical Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, Canada.
authors.forename: Chandrashekhar
authors.initials: C
authors.lastname: Charavaryamath
authors.forename: Vanessa
authors.initials: V
authors.lastname: Juneau
authors.forename: Sarabjeet Singh
authors.initials: SS
authors.lastname: Suri
authors.forename: Kyathanahalli S
authors.initials: KS
authors.lastname: Janardhan
authors.forename: Hugh
authors.initials: H
authors.lastname: Townsend
authors.forename: Baljit
authors.initials: B
authors.lastname: Singh
mesh_terms: D000388:Air; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D000818:Animals; D002465:Cell Movement; D016207:Cytokines / Q000096:biosynthesis; D051379:Mice; D018345:Mice, Knockout; D011014:Pneumonia / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D012130:Respiratory Hypersensitivity / Q000209:etiology*; D013552:Swine; D051197:Toll-Like Receptor 4 / Q000276:immunology*
chemical_list: D016207:Cytokines; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4