[Experimental study on the chitosan-DNA vaccines against campylobacter jejuni invasion].
OBJECTIVE
The immunogenicity and protective efficacy of an experimental Campylobacter jejuni (C. jejuni) chitosan-DNA vaccines were evaluated in mice.


METHODS
The chitosan-DNA vaccines were prepared by embedding pcDNA3.1(+)-cadF and pcDNA3.1(+)-peblA with chitosan respectively. BALB/c mice were intranasally immunized in a four-dose primary series (7 d intervals) at doses of 60 microg chitosan-DNA vaccines each time. The comparative immunogenicities of nine formulations were assessed on the basis of the generation of antigen-specific antibodies in serum and intestinal secretions. Mice were attacked repeatedly through intragastric administration of C. jejuni HS:19 at the 8th week after the immunization and protective efficacy was determined by detecting the degrees of protection afforded against C. jejuni invaded.


RESULTS
The mice immunized with chitosan-DNA vaccines have generated high levels of IgA and IgG from the sera and IgA from the intestinal secretions and the P/N value went up to 20.58, 30.13 and 6.87 respectively. Meanwhile, the expression of intestinal SIgA increased correspondingly. Moreover the chitosan-DNA vaccines induced strongest level of protection in BALB/c mice against challenge with C. jejuni HS:19 strain and the protective efficacies was 93.70.


CONCLUSIONS
The results of this study indicate that the chitosan-DNA vaccines could induce significant protective immunity against C. jejuni challenge in the mice model.
Zheng
Hui
H
Department of Neurology, Children's Hospital, Chongqing Medical University, Chongqing, China.
Cai
Fang-cheng
FC
Zhong
Min
M
Deng
Bing
B
Li
Xin
X
Zhang
Xiao-ping
XP
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D002169:Campylobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016123:Campylobacter jejuni / Q000276:immunology; D048271:Chitosan / Q000276:immunology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology / Q000627:therapeutic use*
D000907:Antibodies, Bacterial; D007071:Immunoglobulin A, Secretory; D019444:Vaccines, DNA; D048271:Chitosan