title: The intestinal epithelial cell modulates the effect of alcohol on neutrophil inflammatory potential.
abstract: BACKGROUND
  Ethanol (EtOH) intoxication increases posttraumatic infectious complications. EtOH is thought to be immunosuppressive, but the effect of the interaction with the gut and associated microflora on host defense is unknown. We studied the ability of intestinal epithelial cells and bacteria to modulate EtOH effects on polymorphonuclear (PMN) cell function in vitro.
  
  
  METHODS
  Confluent Caco2 monolayers were exposed to 0.1% EtOH with or without Escherichia coli on the apical side of each chamber. Supernatants were collected from the basal side of the chambers and incubated with neutrophils. Controls were incubated with or without 0.1% EtOH alone. Chemotaxis, elastase, and superoxide anion release, and CD11b measurements were undertaken in these neutrophils and compared with controls.
  
  
  RESULTS
  Alcohol exhibited differing effects on the inflammatory potential of neutrophils in regards to CD11b expression, elastase production, and superoxide production. Direct effects of EtOH were anti-inflammatory, whereas indirect effects of EtOH on neutrophils modulated by Caco2 cells were proinflammatory. Chemotaxis was significantly reduced when neutrophils were directly treated with alcohol.
  
  
  CONCLUSIONS
  EtOH direct downregulates PMN inflammatory potential, but its impact of the intestinal epithelial cells produces a proinflammatory microenvironment for PMNs. The interaction of EtOH on the intestinal milieu may lead to neutrophil activation and increased organ injury after trauma in the intoxicated patient.
authors.affiliation: Department of Surgery, Wayne State University/University Health Center 6-C, Detroit, Michigan, USA. pamin@dmc.org
authors.forename: Parth B
authors.initials: PB
authors.lastname: Amin
authors.forename: Lawrence N
authors.initials: LN
authors.lastname: Diebel
authors.forename: David M
authors.initials: DM
authors.lastname: Liberati
mesh_terms: D039481:CD11b Antigen / Q000187:drug effects*; D018938:Caco-2 Cells; D002633:Chemotaxis / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000276:immunology; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000276:immunology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D012997:Solvents / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D039481:CD11b Antigen; D012997:Solvents; D013481:Superoxides; D000431:Ethanol; D010196:Pancreatic Elastase