Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes.
The silk protein sericin has been identified as a potent antioxidant and photoprotective agent against ultraviolet B (UVB) irradiation in mouse skin model. In this study, we have investigated the anti-apoptotic effect of sericin in UVB (30 mJ/cm2)-irradiated human keratinocytes. Flow cytometry analysis has shown that pre-treatment with sericin inhibits UVB-induced apoptosis. The pre-treatment with sericin suppresses bax expression, up-regulates the expression of bcl-2, prevents both the activation of caspase-3 and cleavage of Poly (ADP-ribose) polymerase. Generation of intracellular hydrogen peroxide in UVB-treated keratinocytes is inhibited through pre-treatment with sericin suggesting that sericin probably prevents mitochondrial damage.
Dash
Rupesh
R
Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Mandal
Mahitosh
M
Ghosh
Sudip K
SK
Kundu
S C
SC
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D012831:Bombyx / Q000737:chemistry*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004789:Enzyme Activation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D051379:Mice; D016877:Oxidants / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000378:metabolism / Q000494:pharmacology*; D047030:Sericins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*; D014466:Ultraviolet Rays; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D016877:Oxidants; D011837:Radiation-Protective Agents; D047030:Sericins; D051028:bcl-2-Associated X Protein; D006861:Hydrogen Peroxide; D011065:Poly(ADP-ribose) Polymerases; D048031:JNK Mitogen-Activated Protein Kinases; D053148:Caspase 3