Human micro- and macrovessel-derived endothelial cells: a comparative study on the effects of adrenaline and a selective adenosine A2-type receptor agonist under normoxic and hypoxic conditions.
Adrenaline is a highly effective stimulator of cyclic AMP (cAMP) production in microvascular endothelial cells (ECs)--HMEC-1, showing only a moderate activity in macrovascular ECs--HUVEC. In both EC preparations, adrenaline acts via beta-type receptors. Selective stimulation of adenosine A(2)-type receptors resulted in comparable increases in cAMP formation in ECs lining micro- and macrovessels. Hypoxia largely suppressed the cAMP effects resulting from stimulation of both beta-adrenoceptors and adenosine A(2) type receptors in ECs of microvessels (HMEC-1). In contrast, hypoxia had only slight effect on these responses in ECs of macrovessels (HUVEC). The present data provide further evidence of functional differences between microvessel- and macrovessel-derived ECs.
Wiktorowska-Owczarek
Anna
A
Department of Pharmacology, Medical University of Łódź, Zeligowskiego 7/9, PL 90-752 Łódź, Poland.
Namiecinska
Magdalena
M
Balcerczyk
Aneta
A
Nowak
Jerzy Z
JZ
D058908:Adenosine A2 Receptor Agonists; D019830:Adenosine-5'-(N-ethylcarboxamide) / Q000494:pharmacology*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D008833:Microcirculation / Q000166:cytology; D012867:Skin / Q000098:blood supply; D014471:Umbilical Veins / Q000166:cytology
D058908:Adenosine A2 Receptor Agonists; D019830:Adenosine-5'-(N-ethylcarboxamide); D000242:Cyclic AMP; D004837:Epinephrine