(R)-albuterol decreases immune responses: role of activated T cells.
Racemic albuterol is an equimolar mixture of two isomers, (R) and (S). Whether (R) and (S) isomers and the combination of both exert different effects in immune activation is not well defined. We analyzed the effects of (R+S)-albuterol, (R)-albuterol and (S)-albuterol in a murine model of allergic pulmonary inflammation and in activated T cells. Mice (C57BL/6) sensitized and aerosol challenged with the allergen ovalbumin (OVA) or phosphate buffered saline (PBS) were treated with (R)-albuterol, (S)-albuterol or (R+S)-albuterol. Following administration of (R)-albuterol, allergen induced bronchoalveolar lavage eosinophils and IgE showed a decrease, albeit not significantly by ANOVA. As T cells are important in allergic inflammation, we asked whether (R+S), (R) or (S)-albuterol might differ in effects on T cells and on the activity of the inflammatory transcription factor NF-kappaB. In activated T cells, (R)-albuterol administration decreased levels of inflammatory cytokines and NF-kappaB activity. These studies suggest that (R)-albuterol decreases cytokine secretion and NF-kappaB activity in T cells.
Ferrada
Marcela A
MA
Pulmonary and Critical Care Division, University of California San Diego, La Jolla, USA. marceferrada@yahoo.com
Gordon
Erin L
EL
Jen
Kai Yu
KY
He
Hong Zhen
HZ
Lu
Xin
X
Barone
Leesa M
LM
Amirifeli
Sepideh
S
Perkins
David L
DL
Finn
Patricia W
PW
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D005260:Female; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000378:metabolism; D007249:Inflammation; D008171:Lung Diseases / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D010047:Ovalbumin; D011014:Pneumonia / Q000276:immunology* / Q000473:pathology; D020033:Protein Isoforms / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
D000318:Adrenergic beta-Agonists; D016328:NF-kappa B; D020033:Protein Isoforms; D007073:Immunoglobulin E; D010047:Ovalbumin; D000420:Albuterol