title: Thermal Characterization of Purified Glucose Oxidase from A Newly Isolated Aspergillus Niger UAF-1.
abstract: An intracellular glucose oxidase was isolated from the mycelium extract of a locally isolated strain of Aspergillus niger UAF-1. The enzyme was purified to a yield of 28.43% and specific activity of 135 U mg(-1) through ammonium sulfate precipitation, anion exchange and gel filtration chromatography. The enzyme showed high affinity for D-glucose with a Km value of 2.56 mM. The enzyme exhibited optimum catalytic activity at pH 5.5. Temperature optimum for glucose oxidase, catalyzed D-glucose oxidation was 40 degrees C. The enzyme showed a high thermostability having a half-life 30 min, enthalpy of denaturation 99.66 kJ mol(-1) and free energy of denaturation 103.63 kJ mol(-1). These characteristics suggest the use of glucose oxidase from Aspergillus niger UAF-1 as an analytical reagent and in the design of biosensors for clinical, biochemical and diagnostic assays.
authors.affiliation: Department of Chemistry (Biochemistry), University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Anjum Zia
authors.lastname: Khalil-Ur-Rahman
authors.forename: Muhammad
authors.initials: M
authors.lastname: K Saeed
authors.forename: Fozia
authors.initials: F
authors.lastname: Andaleeb
authors.forename: Muhammad
authors.initials: M
authors.lastname: I Rajoka
authors.forename: Munir
authors.initials: M
authors.lastname: A Sheikh
authors.forename: Iftikhar
authors.initials: I
authors.lastname: A Khan
authors.forename: Azeem
authors.initials: A
authors.lastname: I Khan