title: Nocardioides hankookensis sp. nov., isolated from soil.
abstract: A Gram-positive, non-motile, rod-shaped bacterial strain, DS-30(T), was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain DS-30(T) grew optimally at pH 6.0-7.0 and 25 degrees C in the presence of 0-0.5 % (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-30(T) fell within the genus Nocardioides, forming a coherent cluster with the type strains of Nocardioides aquiterrae and Nocardioides pyridinolyticus, with which it exhibited 16S rRNA gene sequence similarity values of 98.1 and 98.3 %, respectively. Strain DS-30(T) exhibited 16S rRNA gene sequence similarity values below 96.8 % with respect to the type strains of the other Nocardioides species. The chemotaxonomic properties of strain DS-30(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16 : 0), C(17 : 1)omega8c and C(18 : 1)omega9c. The DNA G+C content was 71.3 mol%. Strain DS-30(T) exhibited DNA-DNA relatedness levels of 19 and 22 % with respect to the type strains of N. aquiterrae and N. pyridinolyticus, respectively, and could be differentiated from these species with reference to a number of phenotypic characteristics. On the basis of the data obtained, strain DS-30(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides hankookensis sp. nov. is proposed. The type strain is DS-30(T) (=KCTC 19246(T) =CCUG 54522(T)).
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.kr
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kang
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Lee
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S