title: Nocardioides halotolerans sp. nov., isolated from soil on Bigeum Island, Korea.
abstract: A strictly aerobic, Gram-positive, motile, coccoid-shaped, halotolerant actinobacterium (10% NaCl, w/v), designated MSL-23T, was isolated from a soil sample on Bigeum Island, Korea. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Nocardioides, with the highest sequence similarity (95.63%) being to Nocardioides kribbensis KCTC 19038T. The major menaquinone was MK-8(H4), and the predominant cellular fatty acids were i-C16:0, ai-C17:0, C18:1 omega 9c and 10-methyl-C16:0. The DNA G+C content was 69.7 mol%. The 16S rRNA gene sequence of strain MSL 23T and its chemotaxonomic properties showed it to be unique in the genus Nocardioides. Phenotypic characteristics distinguished strain MSL-23T from other Nocardioides species. On the basis of the phenotypic, chemotaxonomic and phylogenetic data strain MSL-23T represents a novel species, for which the name Nocardioides halotolerans sp. nov. is proposed, with MSL-23T (=KCTC 19274T=DSM 19273T) as the type strain.
authors.affiliation: Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Syed G
authors.initials: SG
authors.lastname: Dastager
authors.forename: Jae-Chan
authors.initials: JC
authors.lastname: Lee
authors.forename: Yoon-Jung
authors.initials: YJ
authors.lastname: Ju
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000378:metabolism; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride