Alpha-adrenergic inhibition increases collateral circuit conductance in rats following acute occlusion of the femoral artery.
This study evaluated whether alpha-adrenergic activation contributes to collateral circuit vascular resistance in the hindlimb following acute unilateral occlusion of the femoral artery in rats. Blood pressures (BPs) were measured above (caudal artery) and below (distal femoral artery) the collateral circuit. Arterial BPs were reduced (15-35 mmHg) with individual (prazosin, rauwolscine) or combined (phentolamine) alpha-receptor inhibition. Blood flows (BFs) were measured using microspheres before and after alpha inhibition during the same treadmill speed. alpha(1) inhibition increased blood flow by approximately 40% to active muscles that were not affected by femoral occlusion, whereas collateral-dependent BFs to the calf muscles were reduced by 29 +/- 8.4% (P < 0.05), due to a decrease in muscle conductance with no change in collateral circuit conductance. alpha(2) inhibition decreased both collateral circuit (39 +/- 6.0%; P < 0.05) and calf muscle conductance (36 +/- 7.3%; P < 0.05), probably due to residual alpha(1) activation, since renal BF was markedly reduced with rauwolscine. Most importantly, inhibiting alpha(2) receptors in the presence of alpha(1) inhibition increased (43 +/- 12%; P < 0.05) collateral circuit conductance. Similarly, non-selective alpha inhibition with phentolamine increased collateral conductance (242 +/- 59%; P < 0.05). We interpret these findings to indicate that both alpha(1)- and alpha(2)-receptor activation can influence collateral circuit resistance in vivo during the high flow demands caused by exercise. Furthermore, we observed a reduced maximal conductances of active muscles that were ischaemic. Our findings imply that in the presence of excessive sympathetic activation, which can occur in the condition of intermittent claudication during exertion, an exaggerated vasoconstriction of the existing collateral circuit and active muscle will occur.
Taylor
Jessica C
JC
Department of Biomedical Sciences, E102 Vet. Medical Bldg, University of Missouri, Columbia, MO 65211, USA.
Li
Zeyi
Z
Yang
H T
HT
Laughlin
M Harold
MH
Terjung
Ronald L
RL
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology; D005263:Femoral Artery / Q000294:innervation* / Q000502:physiology*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000502:physiology; D005082:Physical Exertion / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000502:physiology*; D014655:Vascular Resistance / Q000502:physiology; D014661:Vasoconstriction / Q000502:physiology*
D011942:Receptors, Adrenergic, alpha