Enzymatically catalyzed DNA synthesis using L-Asp-dGMP, L-Asp-dCMP, and L-Asp-dTMP.
The replacement of the pyrophosphate moiety of deoxynucleoside triphosphates by L-aspartic acid allows incorporation of natural deoxynucleosides into DNA using HIV reverse transcriptase (RT) as enzyme, while retaining the canonical base-pair selectivity. N-Methylation of the L-aspartic acid leaving group results in a reduced fidelity of incorporation.
Terrazas
Montserrat
M
Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven. montseterrazas@msn.com
Marlière
Philippe
P
Herdewijn
Piet
P
D001224:Aspartic Acid / Q000737:chemistry*; D002384:Catalysis; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry; D003843:Deoxycytidine Monophosphate / Q000737:chemistry*; D003848:Deoxyguanine Nucleotides / Q000737:chemistry*; D054303:HIV Reverse Transcriptase / Q000737:chemistry*; D008745:Methylation; D008968:Molecular Conformation; D015394:Molecular Structure; D013237:Stereoisomerism; D013938:Thymidine Monophosphate / Q000737:chemistry*
D003848:Deoxyguanine Nucleotides; D003843:Deoxycytidine Monophosphate; D001224:Aspartic Acid; D013938:Thymidine Monophosphate; C007257:2'-deoxyguanosine 5'-phosphate; D004247:DNA; D054303:HIV Reverse Transcriptase