Isolation and analysis of genes specifically expressed during basidiomatal development in Antrodia cinnamomea by subtractive PCR and cDNA microarray.
cDNAs specifically expressed at the basidiome stage were isolated by using PCR-selected cDNA subtraction in order to study gene regulation during porous-hymenium basidiomatal formation in Antrodia cinnamomea. blastx results suggested that most of the expressed sequence tags (52.4-69.5%) had no significant protein homology to genes from other published living things. cDNAs particularly expressed at different growing conditions were identified using cDNA microarray analysis. Reverse transcriptase PCR analyses confirmed that the clone putative to P-type ATPase, various cytochrome P450s and some unknown genes were abundant at natural basidiomes while endoglucanase was abundant at the tissue from artificial medium.
Chu
Fang-Hua
FH
School of Forestry and Resource Conservation, National Taiwan University, Taipei, Taiwan.
Lee
Yi-Ru
YR
Chou
Shu-Jen
SJ
Chang
Tun-Tschu
TT
Shaw
Jei-Fu
JF
D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005800:Genes, Fungal / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020072:Polyporales / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013382:Subtraction Technique; D014158:Transcription, Genetic / Q000235:genetics
D012333:RNA, Messenger