title: Clinical significance of nuclear non-phosphorylated beta-catenin in acute myeloid leukaemia and myelodysplastic syndrome.
abstract: Wnt signaling activates the canonical pathway and induces the accumulation of non-phosphorylated beta-catenin (NPBC) in the nucleus. Although this pathway plays an important role in the maintenance of haematopoietic stem cells as well as in oncogenesis, the significance of nuclear NPBC remains unclear in malignant haematopoiesis. This study examined the expression of nuclear NPBC in bone marrow specimens from 54 and 44 patients with de novo acute myeloid leukaemia (AML) and myelodysplastic syndrome (MDS), respectively. On immunohistochemistry with an anti-NPBC antibody, the nuclei were positively stained in 22 and 18 of AML and MDS specimens, respectively. Staining of nuclear NPBC was associated with AML subtypes (M6 and M7), low complete remission (CR) rate, and poor prognosis. Nuclear NPBC was also associated with a high score when using the International Prognostic Scoring System (IPSS) for MDS and with -7/-7q and complex karyotypes. These findings suggest that in situ detection of nuclear NPBC by immunohistochemistry could provide new insights into the pathogenesis and prognosis of AML and MDS.
authors.affiliation: Department of Haematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan. xuj@med.nagoya-u.ac.jp
authors.forename: Jinglan
authors.initials: J
authors.lastname: Xu
authors.forename: Momoko
authors.initials: M
authors.lastname: Suzuki
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Niwa
authors.forename: Junji
authors.initials: J
authors.lastname: Hiraga
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Nagasaka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Ito
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Akihiro
authors.initials: A
authors.lastname: Tomita
authors.forename: Akihiro
authors.initials: A
authors.lastname: Abe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kiyoi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Tomoki
authors.initials: T
authors.lastname: Naoe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism; D010766:Phosphorylation; D011379:Prognosis; D016019:Survival Analysis; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D051176:beta Catenin