[Effect of ionic and colloid gold on ATP-hydrolase fermentative systems in the membrane of Bacillus sp. B4253 and Bacillus sp. B4851].
Accumulation of gold in cells of Bacillus sp. B4253 can be directly or indirectly connected with activity of bacteria plasma membrane basal Mg2+-ATPase. Therefore this work deals with a comparative analysis of kinetic properties of plasma membrane basal azide-resistant Mg2+-dependent ATP-hydrolase activity of B. sp. B4253 and B. sp. B4851 capable to gold accumulation and not capable to this process, accordingly. It is shown, that by a number of kinetic parameters - specific fermentative activity, initial speed of reaction of hydrolysis ATP (V0), Mixaelis constant (Km), the maximal initial speed by Mg2+ (V(Mg)) and by ATP (V(ATP)), optimum concentration of ATP ([ATP]opt), pHmax, sensitivity to action of the thapsigargine and eosine Y - bacteria membranes basal Mg2+-ATPase activity accumulating gold, and the bacteria not capable to this process, are identical. But by some parameters they differ: Mg2+-ATPase activity of membranes of the bacteria which do not accumulate gold, has three times greater affinity for Mg ions and smaller value [Mg]opt. The inhibition effect of ionic gold (10(-4)-3x10(-4) M) is shown on azide-sensitive (H+-ATPase) and azide-resistant (Mg2+-ATPase) components Mg2+-dependent ATP-hydrolase activity in fraction of plasma membranes of microorganisms Bacillus accumulating gold, and not capable to this process. Colloid gold (0.0002-4 microg/ml) stimulates activity of H+-ATPase and Mg2+-ATPase in a membrane of the bacteria accumulating gold 1.5-2 times, and does not influence activity of ATPases of a membrane of the bacteria which do. not accumulate gold.
Danylovych
H V
HV
Hruzina
T H
TH
Ul'berh
Z R
ZR
Kosterin
S O
SO
D001407:Bacillus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002384:Catalysis; D002412:Cations; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology; D017979:Gold Colloid / Q000378:metabolism / Q000494:pharmacology*; D017612:Gold Compounds / Q000378:metabolism / Q000494:pharmacology*; D019284:Thapsigargin / Q000494:pharmacology
D002412:Cations; D004791:Enzyme Inhibitors; D017979:Gold Colloid; D017612:Gold Compounds; C038016:gold chloride; D019284:Thapsigargin; D017301:Ca(2+) Mg(2+)-ATPase; D004801:Eosine Yellowish-(YS)