title: El Tor and Calcutta CTXPhi precursors coexisting with intact CTXPhi copies in Vibrio cholerae O139 isolates.
abstract: Restriction fragment length polymorphism analyses of the array of CTXPhi prophages in strains CRC262 and CRC266 of Vibrio cholerae O139 revealed the presence of copies of complete CTXPhi and pre-CTXPhi prophages coexisting at a single chromosomal locus in each strain. Restriction pattern and comparative nucleotide sequence analysis revealed pre-CTXPhi precursors of both the El Tor and Calcutta lineages. Thus, we hypothesize that two precursor variants independently acquired cholera toxin genes and gave rise to the current El Tor and Calcutta CTXPhi prophages. We discuss the implications of these results in terms of the evolution and origin of the current diversity of CTXPhi prophages.
authors.affiliation: Departamento de Genética, Centro Nacional de Investigaciones Científicas, Ave 25 y 158, Playa, AP 6412, 10600 La Habana, Cuba. talena.ledon@cnic.edu.cu
authors.forename: Talena
authors.initials: T
authors.lastname: Ledón
authors.forename: Javier
authors.initials: J
authors.lastname: Campos
authors.forename: Edith
authors.initials: E
authors.lastname: Suzarte
authors.forename: Boris
authors.initials: B
authors.lastname: Rodríguez
authors.forename: Karen
authors.initials: K
authors.lastname: Marrero
authors.forename: Rafael
authors.initials: R
authors.lastname: Fando
mesh_terms: D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D018628:Gene Dosage; D007194:India; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D039002:Prophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D041242:Vibrio cholerae O139 / Q000821:virology*; D016662:Virus Integration
chemical_list: D004279:DNA, Viral; D002772:Cholera Toxin