title: Xerogel optical sensor films for quantitative detection of nitroxyl.
abstract: Xerogel sensing films were synthesized via sol-gel chemistry were used to fabricate optical nitroxyl (HNO) sensors [corrected] Selective detection of HNO in solution was achieved by monitoring the rates of manganese(III) meso-tetrakis(4-sulfonatophenyl) porphyrinate (MnIIITPPS) reductive nitrosylation in the anaerobic interior of aminoalkoxysilane-derived xerogel films. Nitroxyl permeability in sensor films deposited in round-bottom 96-well plates was enhanced via incorporation of trimethoxysilyl-terminated poly(amidoamine-organosilicon) dendrimers in the xerogel network. The selectivity of MnIIITPPS for HNO, the overall sensitivity, and the working dynamic range of the resulting sensors were characterized. The HNO-sensing microtiter plates were used to quantify pH-dependent HNO generation by the recently described HNO-donor sodium 1-(isopropylamino)diazene-1-ium-1,2-diolate (IPA/NO), and compare HNO production efficiency between IPA/NO and Angeli's salt, a traditional HNO-donor.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3290, USA.
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Dobmeier
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Riccio
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005782:Gels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008345:Manganese / Q000737:chemistry; D018696:Neuroprotective Agents / Q000032:analysis; D009589:Nitrogen Oxides / Q000032:analysis*; D055095:Optics and Photonics; D009942:Organometallic Compounds / Q000737:chemistry; D017646:Organosilicon Compounds / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D005782:Gels; D018696:Neuroprotective Agents; D009589:Nitrogen Oxides; D009942:Organometallic Compounds; D017646:Organosilicon Compounds; D011166:Porphyrins; D008345:Manganese; D012822:Silicon Dioxide; C039900:nitroxyl