No association between common Gly972Arg variant of the insulin receptor substrate-1 and polycystic ovary syndrome in Southern Chilean women.
BACKGROUND
Previously studies have indicated that the insulin receptor substrate-1 (IRS-1) Gly972Arg (G972R) polymorphism is associated with polycystic ovary syndrome (PCOS). We examined the possible association between G972R common variant of the IRS-1 gene and PCOS in Southern Chilean women with PCOS and controls.


METHODS
A total of 50 women with PCOS (29.1+/-8.1 yr) and 75 healthy women (29.3+/-9.3 yr) were included. Serum lipids, glucose and uric acid concentrations were determined by enzymatic-colorimetric methods. The G972R variant of the IRS-1 gene was detected by PCR-RFLP.


RESULTS
Women with PCOS exhibited a higher concentrations of total testosterone, glucose, insulin, total cholesterol, triglycerides, LDL-C and uric acid, and lower HDL-C concentrations than controls (P<0.05). The presence of G972R polymorphism in PCOS and control women was not significantly different (16% vs. 6.6%, P=0.276). The OR for PCOS associated to 972R variant was 2.67 (95% CI: 0.82-8.69, P=NS). Moreover, neither association between G972R genotypes and metabolic parameters were observed in PCOS women or controls.


CONCLUSIONS
Our data do not support an association between G972R variant of the IRS-1 with PCOS or its metabolic parameters in Southern Chilean women.
Valdés
Patricio
P
Departamento de Obstetricia and Ginecología, Facultad de Medicina, Universidad de La Frontera, Av. Manuel Montt 112, Casilla 54-D, Temuco, Chile.
Cerda
Alvaro
A
Barrenechea
Cristobal
C
Kehr
Marlene
M
Soto
Carolina
C
Salazar
Luis A
LA
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D001120:Arginine / Q000235:genetics*; D016022:Case-Control Studies; D002677:Chile; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005998:Glycine / Q000235:genetics*; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D008875:Middle Aged; D011085:Polycystic Ovary Syndrome / Q000235:genetics*
D048868:Adaptor Proteins, Signal Transducing; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D001120:Arginine; D005998:Glycine