Differences of Cu accumulation and Cu-induced ATPase activity in roots of two populations of Elsholtzia haichowensis Sun.
Two populations of Elsholtzia haichowensis, which are from an uncontaminated site (Hong'an) and a Cu mine site (Tonglushan) in Hubei province, China, were studied for ATPase activities and Cu accumulation of roots under hydroponic conditions. The root tolerance indices were markedly higher in Tonglushan population than in Hong'an population under Cu stress conditions. The root Cu concentration in Tonglushan population was significantly lower than that in Hong'an population in all Cu treatments. The Cu-stimulated ATPase activity increased significantly under 1 microM Cu, reached a peak at 5 microM Cu and then, began to decrease. However, no significant change of ATPase activity was observed in Hong'an population except for an obvious decrease in 80 microM Cu treatment. These results showed that Cu-ATPase might exist in the root plasma membrane of Tonglushan population and play a possible role in resistance to copper.
Ke
Wenshan
W
School of Life Science, Hubei University, Wuhan 430062, People's Republic of China. kokews@hotmail.com
Xiong
Zhiting
Z
Chen
Shijian
S
Wang
Zhenghe
Z
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002681:China; D003300:Copper / Q000378:metabolism / Q000633:toxicity; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019686:Lamiaceae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism / Q000633:toxicity; D008906:Mining; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism
D004785:Environmental Pollutants; D004791:Enzyme Inhibitors; D019216:Metals, Heavy; D003300:Copper; D000251:Adenosine Triphosphatases