Improving yeast two-hybrid screening systems.
Yeast two-hybrid (Y2H) screening methods are an effective means for the detection of protein-protein interactions. Optimisation and automation has increased the throughput of the method to an extent that allows the systematic mapping of protein-protein interactions on a proteome-wide scale. Since two-hybrid screens fail to detect a great number of interactions, parallel high-throughput approaches are needed for proteome-wide interaction screens. In this review, we discuss and compare different approaches for adaptation of Y2H screening to high-throughput, the limits of the method and possible alternative approaches to complement the mapping of organism-wide protein-protein interactions.
Koegl
Manfred
M
Translational Research, and Genomics and Proteomics Core Facilities, German Cancer Research Center, Im Neuenheimer Feld 515, 69120 Heidelberg, Germany. m.koegl@dkfz.de
Uetz
Peter
P
D016477:Artifacts; D018076:DNA, Complementary; D047468:Immunoprecipitation; D008156:Luciferases / Q000378:metabolism; D040081:Protein Array Analysis; D020798:Two-Hybrid System Techniques
D018076:DNA, Complementary; D008156:Luciferases