Interferon-gamma upregulates retinoic acid-inducible gene-I in human pericardial mesothelial cells.
OBJECTIVE
Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family proteins and serves as an intracellular signalling molecule involved in the regulation of various genes. Mesothelial cells are active in generating a variety of bioactive factors, and RIG-I may serve as a potential intracellular molecule regulating part of these factors. We have addressed the expression of RIG-I in pericardial mesothelial cells.


RESULTS
Cultures of human mesothelial cells separated during cardiac surgery were established. Interferon (IFN)-gamma was found to stimulate the expression, in cultured mesothelial cells, of RIG-I mRNA and protein in time- and concentration-dependent manners. We also performed immunohistochemical analysis of heart tissues to examine the expression of RIG-I in mesothelial cells. Intense immunoreactivity was detected in reactive pericardial mesothelial cells in non-specific pericarditis and in dilated cardiomyopathy.


CONCLUSIONS
RIG-I may be involved in the inflammatory reaction in pericardial mesothelial cells.
Hatakeyama
Masaharu
M
Department of Surgery, Hirosaki University School of Medicine, Hirosaki, Japan.
Imaizumi
Tadaatsu
T
Terasaki
Fumio
F
Mori
Fumiaki
F
Tanji
Kunikazu
K
Sato
Fuyuki
F
Kijima
Hiroshi
H
Suma
Hisayoshi
H
Wakabayashi
Koichi
K
Yoshida
Hidemi
H
Fukuda
Ikuo
I
Satoh
Kei
K
D000818:Animals; D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics; D004848:Epithelium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology*; D010496:Pericardium / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D015854:Up-Regulation / Q000502:physiology*
D018836:Inflammation Mediators; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases