title: Critical role of a single position in the -35 element for promoter recognition by Mycobacterium tuberculosis SigE and SigH.
abstract: Mycobacterial SigE and SigH both initiate transcription from the sigB promoter, suggesting that they recognize similar sequences. Through mutational and primer extension analyses, we determined that SigE and SigH recognize nearly identical promoters, with differences at the 3' end of the -35 element distinguishing between SigE- and SigH-dependent promoters.
authors.affiliation: The Genome Research Center for Respiratory Pathogens, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
authors.forename: Taeksun
authors.initials: T
authors.lastname: Song
authors.forename: Seung-Eun
authors.initials: SE
authors.lastname: Song
authors.forename: Sahadevan
authors.initials: S
authors.lastname: Raman
authors.forename: Mauricio
authors.initials: M
authors.lastname: Anaya
authors.forename: Robert N
authors.initials: RN
authors.lastname: Husson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C120328:SigH protein, bacteria; D012808:Sigma Factor; C111259:sigE protein, Bacteria