title: [Study on the relationship between the expression of SOCS-1/3 in the peripheral blood mononuclear cells in patients with multiple organ dysfunction syndrome and their prognosis].
abstract: OBJECTIVE
  To observe the mRNA and protein expression of suppressor of cytokines signaling protein 1/3 (SOCS-1/3) in the peripheral blood mononuclear cells (PBMCs) in patients with multiple organ dysfunction syndrome (MODS), and to explore the relationship between the SOCS expression and the patients' prognosis.
  
  
  METHODS
  Peripheral blood specimens of 32 patients with MODS and 24 healthy volunteers (controls) were collected and the PBMCs were isolated by centrifugation and Ficoll-Hypaque sedimentation. The mRNA of SOCS-1/3 was determined by reverse transcription-polymerase chain reaction (RT-PCR). And the protein content of SOCS-1/3 was determined by Western blotting. The relationship between the SOCS expression and the patients' prognosis was analyzed.
  
  
  RESULTS
  There was no significant difference in the SOCS-1 mRNA expression between control group and MODS group (0.526+/-0.044 vs. 0.466+/-0.047, P>0.05). The SOCS-1 expression of MODS group was significantly higher than that of control group (0.814+/-0.045 vs. 0.479+/-0.021, P<0.05). In the MODS group, the SOCS-1 mRNA expression and protein content of dead patients were significantly lower than those of survived patients (mRNA 0.487+/-0.032 vs. 0.532+/-0.028, protein 0.787+/-0. 029 vs. 0.838+/-0.040, both P<0.05). There was significant negative correlation between the SOCS-1 mRNA expression and the MODS score (r1=-0.731,P<0.01). There was also significant negative correlation between the SOCS-1 content and the MODS score (r2=-0.526,P<0.01). The SOCS-3 mRNA expression of MODS group was higher than that of control group (0.993+/-0.415 vs. 0.461+/-0.046, P<0.05). The SOCS-3 content in the PBMCs of MODS groups was significant higher than that of control group (0.458+/-0.033 vs. 0.403+/-0. 024, P<0.05). In the MODS group, the SOCS-3 mRNA expression and protein content of dead patients were significant higher than those of survived patients (mRNA 1. 245+/-0.408 vs. 0.805+/-0.326, protein 0.486+/-0.021 vs. 0.425+/-0.016, both P<0.05). There was positive correlation between the SOCS-3 mRNA expression and the MODS score but the correlation was not significant (r=0. 468, P>0.05). And there was significant positive correlation between the SOCS-3 content and the MODS score (r=0.783, P<0.01).
  
  
  CONCLUSIONS
  SOCS-1 may protect the tissue from further damage while SOCS-3 may cause tissue damage indirectly. The detection of SOCS-1/3 may help to predict the prognosis of MODS.
authors.affiliation: Department of General Internal Medicine, The First Affiliated Hospital of SUN Yet-sen University, Guangzhou 510080, Guangdong, China.
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Chen
authors.forename: Yan-bing
authors.initials: YB
authors.lastname: Liang
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Xian-shou
authors.initials: XS
authors.lastname: Lin
authors.forename: Dong-hua
authors.initials: DH
authors.lastname: He
authors.forename: Zhong-fu
authors.initials: ZF
authors.lastname: Ma
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000097:blood* / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C108944:SOCS1 protein, human; C106644:SOCS3 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins