Real-time monitoring primary cardiomyocyte adhesion based on electrochemical impedance spectroscopy and electrical cell-substrate impedance sensing.
The cell-substrate distance is a direct indicator of cell adhesion to extracellular matrix which is indispensable in cell culture. A real-time monitoring approach can provide a detailed profile of cell adhesion, so that enables the detecting of adhesion-related cell behavior. In this work, we report a novel real-time impedance-based method to record the adhesion profile of cardiomyocyte, overcoming its inscrutability due to the primary culture. Microfabricated biosensors are applied in cardiomyocyte culture after characterizing the cell-free system. Cyclic frequency scanning data of cell-related impedance are generated and automatically fit into the equivalent circuit model, which is established using electrochemical impedance spectroscopy. The data are displayed as the alteration of normalized cell-substrate distance and the essential parameters for manual electric cell-substrate impedance sensing calibration of absolute distance. The time course displays a significant decline in the equivalent cell-substrate distance, from 155.8 to 60.2 nm in the first 20 h of cardiomyocyte culture. Furthermore, the cardiomyocytes cultured in long-term medium and short-term medium (ACCT) for 10 h exhibit distinct difference in adhesion rate as well as cell-substrate distance (72 vs 68 nm).
Qiu
Yiling
Y
Laboratory for Microsystems Technology, Department of Manufacturing Engineering, Boston University, Brookline, MA 02446, USA.
Liao
Ronglih
R
Zhang
Xin
X
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002448:Cell Adhesion / Q000502:physiology; D002478:Cells, Cultured; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013057:Spectrum Analysis / Q000379:methods; D013997:Time Factors