Improved high-performance liquid chromatographic detection of paclitaxel in patient's plasma using solid-phase extraction, and semi-micro-bore C18 separation and UV detection.
Although a number of analytical methods for taxanes have been published, none of them are sufficiently suitable for use in a medical setting. In this study, we established an improved analytical HPLC/UV detection method using a Sep-Pak C18 cartridge for extraction and a semi-micro-borecolumn for separation. This method employed here reduced chromatographic background signals, and allowed a more sensitive analysis of taxanes in human blood sample. The recovery of taxanes after the solid-phase extraction procedure was over 90%. Chromatographic separation of paclitaxel and docetaxel was achieved within 30 min with no interference peak by a semi-micro-bore column, packed either with C18 (Wakosil 5C18 RS) or pentafluorophenyl (Curosil/Taxol) materials. The method was reproducible with coefficients of variation less than 6%. This analytical procedure was simple and sensitive with lower quantification limit of 3 ng/ml. The improved sensitivity achieved by the popular HPLC/UV apparatus, which is available in hospitals, would vouch safer and more efficient therapy with taxane.
Suno
Manabu
M
Department of Hospital Pharmacy & Pharmacology, Asahikawa Medical College, Asahikawa 078-8510, Japan.
Ono
Takashi
T
Iida
Shinya
S
Umetsu
Noriko
N
Ohtaki
Ko-ichi
K
Yamada
Takehiro
T
Awaya
Toshio
T
Satomi
Machiko
M
Tasaki
Yoshikazu
Y
Shimizu
Keiko
K
Matsubara
Kazuo
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D005260:Female; D006801:Humans; D017239:Paclitaxel / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D014466:Ultraviolet Rays
D017239:Paclitaxel