title: Burkholderia sartisoli sp. nov., isolated from a polycyclic aromatic hydrocarbon-contaminated soil.
abstract: A Gram-negative, rod-shaped, aerobic bacterium, designated strain RP007(T), was isolated from a polycyclic aromatic hydrocarbon-contaminated soil in New Zealand. Two additional strains were recovered from a compost heap in Belgium (LMG 18808) and from the rhizosphere of maize in the Netherlands (LMG 24204). The three strains had virtually identical 16S rRNA gene sequences and whole-cell protein profiles, and they were identified as members of the genus Burkholderia, with Burkholderia phenazinium as their closest relative. Strain RP007(T) had a DNA G+C content of 63.5 mol% and could be distinguished from B. phenazinium based on a range of biochemical characteristics. Strain RP007(T) showed levels of DNA-DNA relatedness towards the type strain of B. phenazinium and those of other recognized Burkholderia species of less than 30 %. The results of 16S rRNA gene sequence analysis, DNA-DNA hybridization experiments and physiological and biochemical tests allowed the differentiation of strain RP007(T) from all recognized species of the genus Burkholderia. Strains RP007(T), LMG 18808 and LMG 24204 are therefore considered to represent a single novel species of the genus Burkholderia, for which the name Burkholderia sartisoli sp. nov. is proposed. The type strain is RP007(T) (=LMG 24000(T) =CCUG 53604(T) =ICMP 13529(T)).
authors.affiliation: Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Gent, Belgium.
authors.forename: Elke
authors.initials: E
authors.lastname: Vanlaere
authors.forename: Jan Roelof
authors.initials: JR
authors.lastname: van der Meer
authors.forename: Enevold
authors.initials: E
authors.lastname: Falsen
authors.forename: Joana Falcao
authors.initials: JF
authors.lastname: Salles
authors.forename: Evie
authors.initials: E
authors.lastname: de Brandt
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009520:New Zealand; D009693:Nucleic Acid Hybridization; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005227:Fatty Acids; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants