title: Open-tubular capillary electrochromatography using a capillary coated with octadecylamine-capped gold nanoparticles.
abstract: Octadecylamine-capped gold nanoparticles (ODA-Au-NPs) were prepared and characterized by using UV-Vis adsorption spectrum, transmission electron chromatography (TEM), SEM, and FT-IR. A simple but robust hydrophobic coating was easily developed by flushing a capillary with a solution of ODA-Au-NPs, because the positive charges were carried by the nanoparticles which strongly adsorb to the negatively charged inner surface of a fused-silica capillary via electrostatic and hydrophobic interactions. The chromatographic characteristics of the coated capillary was investigated by varying the experimental parameters such as buffer pH, buffer concentration, and percentage of organic modifier in the mobile phase. The results show that (i) resolution between thiourea and naphthalene is almost the same when comparing the electrochromatograms obtained using pH 7 buffer as mobile phase after and before the capillary column was operated using pH 11 and 3 mobile phase; (ii) no significant changes in retention time and deterioration in peak efficiency were found after 60 runs of test aromatic mixtures; and (iii) column efficiency up to 189 000 theoretical plates/meter for testosterone was obtained. All of the results indicated that the coating could act as a stable stationary phase for open tubular CEC as well as for bioanalysis.
authors.affiliation: Jiangsu Key Laboratory of Environmental Materials and Environmental Engineering, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, P. R. China. quqishu@gmail.com
authors.forename: Qishu
authors.initials: Q
authors.lastname: Qu
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Shen
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Hu
authors.forename: Gongjun
authors.initials: G
authors.lastname: Yang
authors.forename: Chengyin
authors.initials: C
authors.lastname: Wang
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
mesh_terms: D000588:Amines / Q000737:chemistry*; D053801:Capillary Electrochromatography / Q000379:methods*; D006046:Gold; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D009281:Naphthalenes / Q000032:analysis; D055672:Static Electricity; D013890:Thiourea / Q000032:analysis
chemical_list: D000588:Amines; D009281:Naphthalenes; C031721:naphthalene; D006046:Gold; C009317:stearylamine; D013890:Thiourea