Antinociceptive activity of n-butanol fraction from MeOH extracts of Paederia scandens in mice.
The n-butanol fraction of the MeOH extract from Paederia scandens was evaluated for antinociceptive activity in mice using chemical and thermal models of nociception. The n-butanol fraction, given orally at doses of 150, 300 and 600 mg/kg produced significant inhibition of chemical nociception induced by intraperitoneal acetic acid and subplantar formalin or capsaicin injections, and of thermal nociception in the tail-flick test and in the hot plate test. More significant inhibition of nociception was observed at a dose of 600 mg/kg. In the pentobarbital sodium-induced sleeping time test and the open-field test, the n-butanol fraction neither significantly enhanced pentobarbital sodium-induced sleeping time nor impaired motor performance, indicating that the observed anti-nociception was unlikely to be due to sedation or motor abnormality. Moreover, the n-butanol fraction-induced anti-nociception in both the capsaicin and formalin tests was insensitive to naloxone or glibenclamide but was significantly antagonized by nimodipine. These results suggest that the n-butanol fraction produced anti-nociception possibly related to nimodipine-sensitive L-type Ca2+ channels, which merits further studies regarding the precise site and mechanism of action. Furthermore, four iridoid glycosides isolated from the n-butanol fraction might be related to its antinociceptive action fraction. Therefore, the effect on analgesic activity of each chemical compound in large quantity might well be considered in a further study.
Chen
Yu-Feng
YF
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, PR China.
Zhang
Hong
H
Zhang
Qiao-Yan
QY
Wu
Jin-Zhong
JZ
Li
Nan
N
Rahman
K
K
Zheng
Han-Chen
HC
Qin
Lu-Ping
LP
D020001:1-Butanol; D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D002211:Capsaicin; D003251:Constriction, Pathologic / Q000139:chemically induced / Q000517:prevention & control; D005557:Formaldehyde; D006358:Hot Temperature; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D000432:Methanol; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D010147:Pain Measurement / Q000187:drug effects; D010424:Pentobarbital / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011930:Reaction Time / Q000187:drug effects; D019666:Rubiaceae / Q000737:chemistry*; D012890:Sleep / Q000187:drug effects; D012997:Solvents
D000700:Analgesics; D000701:Analgesics, Opioid; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D012997:Solvents; D005557:Formaldehyde; D009020:Morphine; D020001:1-Butanol; D010424:Pentobarbital; D019342:Acetic Acid; D002211:Capsaicin; D000432:Methanol