Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata): the importance of particle solubility.
Metal oxide nanoparticles are finding increasing application in various commercial products, leading to concerns for their environmental fate and potential toxicity. It is generally assumed that nanoparticles will persist as small particles in aquatic systems and that their bioavailability could be significantly greater than that of larger particles. The current study using nanoparticulate ZnO (ca. 30 nm) has shown that this is not always so. Particle characterization using transmission electron microscopy and dynamic light scattering techniques showed that particle aggregation is significant in a freshwater system, resulting in flocs ranging from several hundred nanometers to several microns. Chemical investigations using equilibrium dialysis demonstrated rapid dissolution of ZnO nanoparticles in a freshwater medium (pH 7.6), with a saturation solubility in the milligram per liter range, similar to that of bulk ZnO. Toxicity experiments using the freshwater alga Pseudokirchneriella subcapitata revealed comparable toxicity for nanoparticulate ZnO, bulk ZnO, and ZnCl2, with a 72-h IC50 value near 60 microg Zn/ L, attributable solely to dissolved zinc. Care therefore needs to be taken in toxicity testing in ascribing toxicity to nanoparticles per se when the effects may be related, at least in part, to simple solubility.
Franklin
Natasha M
NM
Centre for Environmental Contaminants Research, CSIRO Land and Water, Private Mail Bag 7, Bangor, NSW, Australia 2234. Natasha.Franklin@csiro.au
Rogers
Nicola J
NJ
Apte
Simon C
SC
Batley
Graeme E
GE
Gadd
Gerald E
GE
Casey
Philip S
PS
D002712:Chlorides / Q000737:chemistry / Q000633:toxicity*; D056890:Eukaryota / Q000187:drug effects*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures; D010316:Particle Size; D012542:Scattering, Radiation; D012995:Solubility; D017967:Zinc Compounds / Q000737:chemistry / Q000633:toxicity*; D015034:Zinc Oxide / Q000737:chemistry / Q000633:toxicity*
D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D015034:Zinc Oxide