Molecular epidemiology and evolution of avian infectious bronchitis virus in Spain over a fourteen-year period.
An in-depth molecular study of infectious bronchitis viruses (IBV) with particular interest in evolutionary aspects of IBV in Spain was carried out in the present study based on the S1 gene molecular characterization of twenty-six Spanish strains isolated over a fourteen-year period. Four genotypes were identified based on S1 gene sequence analyses and phylogenetic studies. A drastic virus population shift was demonstrated along time and the novel Italy 02 serotype was shown to have displaced the previous predominant serotype 4/91 in the field. Detailed analyses of synonymous to non-synonymous ratio of the S1 gene sequences of this new serotype Italy 02 suggested positive selection pressures might have contributed to the successful establishment of Italy 02 serotype in our country. In addition, differences on the fitness abilities of new emergent genotypes were indicated. Furthermore, intergenic sequences (IGs)-like motifs within S1 gene sequences of IBV isolates were suggested to enhance the recombination abilities of certain serotypes.
Dolz
Roser
R
Centre de Recerca en Sanitat Animal (CReSA), Esfera UAB, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. roser.dolz@cresa.uab.cat
Pujols
Joan
J
Ordóñez
German
G
Porta
Ramon
R
Majó
Natàlia
N
D000818:Animals; D002645:Chickens; D016000:Cluster Analysis; D018352:Coronavirus Infections / Q000453:epidemiology* / Q000821:virology*; D019143:Evolution, Molecular; D005838:Genotype; D001351:Infectious bronchitis virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D008562:Membrane Glycoproteins; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology* / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013030:Spain / Q000453:epidemiology; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins
D008562:Membrane Glycoproteins; D012367:RNA, Viral; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins