title: The crystal structure of the putative peptide-binding fragment from the human Hsp40 protein Hdj1.
abstract: BACKGROUND
  The mechanism by which Hsp40 and other molecular chaperones recognize and interact with non-native polypeptides is a fundamental question. How Hsp40 co-operates with Hsp70 to facilitate protein folding is not well understood. To investigate the mechanisms, we determined the crystal structure of the putative peptide-binding fragment of Hdj1, a human member of the type II Hsp40 family.
  
  
  RESULTS
  The 2.7A structure reveals that Hdj1 forms a homodimer in the crystal by a crystallographic two-fold axis. The Hdj1 dimer has a U-shaped architecture and a large cleft is formed between the two elongated monomers. When compared with another Hsp40 Sis1 structure, the domain I of Hdj1 is rotated by 7.1 degree from the main body of the molecule, which makes the cleft between the two Hdj1 monomers smaller that that of Sis1.
  
  
  CONCLUSIONS
  This structural observation indicates that the domain I of Hsp40 may possess significant flexibility. This flexibility may be important for Hsp40 to regulate the size of the cleft. We propose an "anchoring and docking" model for Hsp40 to utilize the flexibility of domain I to interact with non-native polypeptides and transfer them to Hsp70.
authors.affiliation: Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA. hujunbin@uab.edu
authors.forename: Junbin
authors.initials: J
authors.lastname: Hu
authors.forename: Yunkun
authors.initials: Y
authors.lastname: Wu
authors.forename: Jingzhi
authors.initials: J
authors.lastname: Li
authors.forename: Xinguo
authors.initials: X
authors.lastname: Qian
authors.forename: Zhengqing
authors.initials: Z
authors.lastname: Fu
authors.forename: Bingdong
authors.initials: B
authors.lastname: Sha
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D050956:HSP40 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D016415:Sequence Alignment
chemical_list: C494647:DNAJB1 protein, human; D050956:HSP40 Heat-Shock Proteins; D010455:Peptides