title: Design and validation of a dynamic flow perfusion bioreactor for use with compliant tissue engineering scaffolds.
abstract: In tissue engineering, flow perfusion bioreactors can be used to enhance nutrient diffusion while mechanically stimulating cells to increase matrix production. The goal of this study was to design and validate a dynamic flow perfusion bioreactor for use with compliant scaffolds. Using a non-permanent staining technique, scaffold perfusion was verified for flow rates of 0.1-2.0 mL/min. Flow analysis revealed that steady, pulsatile and oscillatory flow profiles were effectively transferred from the pump to the scaffold. Compared to static culture, bioreactor culture of osteoblast-seeded collagen-GAG scaffolds led to a 27-34% decrease in cell number but stimulated an 800-1200% increase in the production of prostaglandin E(2), an early-stage bone formation marker. This validated flow perfusion bioreactor provides the basis for optimisation of bioreactor culture in tissue engineering applications.
authors.affiliation: Department of Anatomy, Royal College of Surgeons in Ireland, 123 St. Stephen's Green, Dublin 2, Ireland.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Jaasma
authors.forename: Niamh A
authors.initials: NA
authors.lastname: Plunkett
authors.forename: Fergal J
authors.initials: FJ
authors.lastname: O'Brien
mesh_terms: D016475:3T3 Cells; D000818:Animals; D019149:Bioreactors; D015232:Dinoprostone / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D015232:Dinoprostone