Time-related changes in expression of collagen types I and III and of tenascin-C in rat bone mesenchymal stem cells under co-culture with ligament fibroblasts or uniaxial stretching.
Adult bone-marrow-derived mesenchymal stroma cells (BMSC) seem to be a potential cell source for tissue engineering of the ligament. The objective of this work was to study the time-related changes in mRNA expression and protein levels of collagen types I and III and of tenascin-C in BMSC under co-culture with fibroblasts or under a uniaxial cyclic condition. Rat BMSC harvested from the femur and tibial bone marrow were co-cultured with ligament fibroblasts or stimulated by cyclic 10% uniaxial stretching at 1 Hz. Image analysis showed significant cell loss in stretched BMSC, particularly in the directions close to the stretching direction. However, these BMSC displayed an equivalent growth rate to that of non-stretched cells. Real-time reverse transcription/polymerase chain reaction revealed that the mRNA expression of collagen types I and III and of tenascin-C by BMSC was significantly up-regulated by co-culture and cyclic stretching. Radioimmunoassay results confirmed the effects of these stimulations, showing increases in the level of these proteins. Thus, BMSC might be useful as a cell source for the tissue engineering of ligament.
Zhang
Lei
L
LEMTA, Nancy University, CNRS, 2 Avenue de la Forêt de Haye, BP 160, 54504 Vandoeuvre-les-Nancy Cedex, France.
Tran
Nguyen
N
Chen
Huai-Qing
HQ
Kahn
Cyril J-F
CJ
Marchal
Sophie
S
Groubatch
Frederique
F
Wang
Xiong
X
D000818:Animals; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells / Q000166:cytology; D002452:Cell Count; D049109:Cell Proliferation; D048430:Cell Shape; D018920:Coculture Techniques; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology*; D005786:Gene Expression Regulation; D008022:Ligaments / Q000166:cytology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D019063:Tenascin / Q000378:metabolism*; D013997:Time Factors
D024042:Collagen Type I; D024061:Collagen Type III; D019063:Tenascin