title: Expression of natriuretic peptide-activated guanylate cyclases by cholinergic and dopaminergic amacrine cells of the rat retina.
abstract: Recently, the natriuretic peptides were detected in the cholinergic and dopaminergic amacrine cells of the retina. We performed immunofluorescence labeling of rat retinal sections to examine the immunoreactivity of natriuretic peptide-activated guanylate cyclases (NPR-A and NPR-B) in the rat retina, in particular whether they were localized to dopaminergic and cholinergic amacrine cells. NPR-A and NPR-B immunoreactivity was detected in several layers of the retina including amacrine cells. In amacrine cells, both NPR-A and NPR-B were co-localized with tyrosine hydroxylase, a marker of dopaminergic cells. NPR-B, but not NPR-A, was localized to amacrine cells expressing choline acetyltransferase (ChAT), a marker of cholinergic cells. These findings suggest that natriuretic peptides have different regulatory systems in dopaminergic and cholinergic amacrine cells in rat retina.
authors.affiliation: Molecular Neuroscience Research Center, Shiga University of Medical Science, Seta, Otsu, Shiga 520-2192, Japan.
authors.forename: Essam Mohamed
authors.initials: EM
authors.lastname: Abdelalim
authors.forename: Chiaki
authors.initials: C
authors.lastname: Masuda
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tooyama
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D025042:Amacrine Cells / Q000201:enzymology*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008297:Male; D045265:Natriuretic Peptides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000032:analysis / Q000378:metabolism*
chemical_list: D045265:Natriuretic Peptides; D002795:Choline O-Acetyltransferase; D017461:Receptors, Atrial Natriuretic Factor; D000109:Acetylcholine; D004298:Dopamine