Aspergillus section Fumigati: antifungal susceptibility patterns and sequence-based identification.
This study analyzed 28 Aspergillus strains belonging to the section Fumigati that were isolated from clinical samples in Spain. All isolates sporulated slowly and were unable to grow at 48 degrees C. Phylogenetic analysis based on sequencing of partial sequences of the beta-tubulin and rodlet A genes was used to classify the 28 strains into six different clades (Neosartorya hiratsukae, Neosartorya pseudofischeri, Aspergillus viridinutans, Aspergillus lentulus, Aspergillus fumigatiaffinis, and Aspergillus fumisynnematus). Antifungal susceptibility testing showed heterogeneous patterns and grouped the strains together by species. Most A. lentulus and A. fumigatiaffinis isolates showed high MICs of amphotericin B (geometric mean [GM] MICs, >or=4.5 microg/ml), itraconazole (GM MICs, >or=6 microg/ml), voriconazole (GM MICs, >or=3 microg/ml), and ravuconazole (GM MICs, >or=3 microg/ml); N pseudofischeri and A. viridinutans showed high MICs of itraconazole (GM MICs, >or=8 microg/ml), voriconazole (GM MICs, >or=3.33 microg/ml), and ravuconazole (GM MICs, >or=2 microg/ml); and N. hiratsukae and A. fumisynnematus were susceptible to all the antifungals tested. In conclusion, a number of different species whose morphological features resemble those of Aspergillus fumigatus could succeed in producing invasive infections in the susceptible host. In addition, some of them showed high MICs for most of the antifungals available for the treatment of patients infected with these organisms. The epidemiology and clinical relevance of these species should therefore be addressed.
Alcazar-Fuoli
Laura
L
Servicio de Micologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km 2, Madrid 28220, Spain.
Mellado
Emilia
E
Alastruey-Izquierdo
Ana
A
Cuenca-Estrella
Manuel
M
Rodriguez-Tudela
Juan L
JL
D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics
D000935:Antifungal Agents; D005656:Fungal Proteins; D009687:Nuclear Proteins; D014404:Tubulin