title: AOP-1 interacts with cardiac-specific protein kinase TNNI3K and down-regulates its kinase activity.
abstract: In the present study, a yeast two-hybrid screening system was used to identify the interaction partners of cardiac troponin I-interacting kinase (TNNI3K) that might serve as regulators or targets, and thus in turn to gain some insights on the roles of TNNI3K. After screening the adult heart cDNA library with a bait construct encoding the ANK motif of TNNI3K, antioxidant protein 1 (AOP-1) was isolated. The interaction between TNNI3K and AOP-1 was confirmed by the in vitro binding assay and coexpression experiments in vivo. The colocalization of TNNI3K and AOP-1 was clarified by confocal immunofluorescence. Moreover, coexpression of AOP-1 inhibited TNNI3K kinase activity in the in vitro kinase assay.
authors.affiliation: Department of Pathology, Medical College, Tongji University, Shanghai, 200092, China. shuangfeiyan888@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Feng
authors.forename: Dong-Qing
authors.initials: DQ
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Cao
authors.forename: Lai-Yuan
authors.initials: LY
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Shi
authors.forename: Xian-Min
authors.initials: XM
authors.lastname: Meng
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D066298:In Vitro Techniques; D020930:MAP Kinase Kinase Kinases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D061105:Peroxiredoxin III; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D017346:Protein Serine-Threonine Kinases; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: D000975:Antioxidants; D017931:DNA Primers; D011994:Recombinant Proteins; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III; D054464:Peroxiredoxins; D017346:Protein Serine-Threonine Kinases; C481078:TNNI3K protein, human; D020930:MAP Kinase Kinase Kinases