Evaluation of recombinant modified vaccinia Ankara virus-based rhesus cytomegalovirus vaccines in rhesus macaques.
A vaccine consisting of rhesus cytomegalovirus (RhCMV) pp65-2, gB and IE1 expressed via modified vaccinia Ankara (MVA) was evaluated in rhesus macaques with or without prior priming with expression plasmids for the same antigens. Following two MVA treatments, comparable levels of anti-gB, pp65-2 and neutralizing antibody responses, and pp65-2- and IE1-specific cellular immune responses were detected in both vaccinated groups. Similar reductions in plasma peak viral loads were observed in these groups compared to untreated controls. This study demonstrates the immunogenicity and protective efficacy of rMVA-based RhCMV subunit vaccines in a primate host and warrants further investigation to improve the efficacy of subunit vaccines against CMV.
Yue
Yujuan
Y
Center for Comparative Medicine, University of California-Davis, County Rd 98 & Hutchison Dr., Davis, CA 95616, USA. yyue@ucdavis.edu
Wang
Zhongde
Z
Abel
Kristina
K
Li
Jinliang
J
Strelow
Lisa
L
Mandarino
Angelo
A
Eberhardt
Meghan K
MK
Schmidt
Kimberli A
KA
Diamond
Don J
DJ
Barry
Peter A
PA
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology; D022482:Cytomegalovirus Vaccines / Q000235:genetics* / Q000276:immunology*; D007117:Immunization, Secondary; D008253:Macaca mulatta; D009500:Neutralization Tests; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA; D022223:Vaccines, Subunit / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics; D019562:Viral Load; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D022482:Cytomegalovirus Vaccines; D019444:Vaccines, DNA; D022223:Vaccines, Subunit; D014764:Viral Proteins