title: Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation.
abstract: A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mug of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
authors.affiliation: Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5-2, 1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D010957:Plasmids / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D004271:DNA, Fungal