title: Correlation between the genetic diversity and variation of total phenolic acids contents in Fructus Xanthii from different populations in China.
abstract: Fructus Xanthii (Cang-Er-Zi) is a traditional Chinese medicine that is used in curing nasal diseases and headache according to the Chinese Pharmacopoeia. For the effective quality control of its medicinal values, reflected by chemical variation patterns, in addition to the relationship with genetic diversity, analyses based on UV spectrophotometry, HPLC fingerprinting and inter-simple sequence repeat (ISSR) molecular markers were carried out, involving 16 Xanthium populations from different locations in China. The HPLC data showed considerable variation of chemical constituents among the 16 Xanthium populations, and they were classified to three chemotypes by hierarchical clustering analysis. Abundant genetic diversity was detected among the Xanthium populations, which were also clustered into three groups based on their ISSR data and varied according to different species. Combining the genetic divergence and chemical differences showed an important result that, in the two chemotypes, the higher contents of total phenolic acids (TPA) in Fructus Xanthii showed greater genetic diversity (I). We suggest that genetic diversity affects the contents of TPA. Since variable phenolic acid contents may affect therapeutic efficacy, it is important to point out that combining the use of genetic base with chemotype will help control the favourable chemotypes and breed new cultivars with more desirable chemical constituents.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai 200433, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Shan-Yong
authors.initials: SY
authors.lastname: Zhou
authors.forename: Hui-Liang
authors.initials: HL
authors.lastname: Li
authors.forename: Qiao-Yan
authors.initials: QY
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Bao-Kang
authors.initials: BK
authors.lastname: Huang
authors.forename: Khalid
authors.initials: K
authors.lastname: Rahman
authors.forename: Han-Chen
authors.initials: HC
authors.lastname: Zheng
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Qin
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D005843:Geography; D062385:Hydroxybenzoates / Q000032:analysis*; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D013056:Spectrophotometry, Ultraviolet; D031210:Xanthium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018744:DNA, Plant; D062385:Hydroxybenzoates; C017616:phenolic acid