Determination of terephthalic acid isopropylamide in urine with a liquid chromatography/mass spectrometry (LC/MS) method.
A sensitive and simple liquid chromatography/mass spectrometry (LC/MS) method was developed for the determination of terephthalic acid isopropylamide, the final metabolite of procarbazine in human urine. A solid-phase extraction with C(18) cartridges was used followed by LC/MS with a single mass spectrometer (SSQ 7000 from Finnigan). Terephthalic acid isobutylamide was the internal standard. The quantification limit was 30 ng/mL in urine (6 x noise). This assay was applied for drug monitoring of terephthalic acid isopropylamide in urine after oral administration of procarbazine in children and adolescents with Hodgkin lymphomas.
Baumann
F
F
Institute of Clinical Pharmacology, University of Leipzig, Leipzig, Germany. F.Baumann@medizin.uni-leipzig.de
Mauz-Körholz
C
C
Clauss
D
D
Borrmann
S
S
Giannis
A
A
Merkel
N
N
Körholz
D
D
Preiss
R
R
D000293:Adolescent; D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring; D004355:Drug Stability; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010795:Phthalic Acids / Q000737:chemistry / Q000652:urine*; D011344:Procarbazine / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
D010795:Phthalic Acids; D011344:Procarbazine; C011363:terephthalic acid; C528395:terephthalic acid isopropylamide