Pulsed-field gel electrophoresis for the discrimination of Oenococcus oeni isolates from different wine-growing regions in Germany.
Reliable techniques are needed for the identification individual Oenococcus oeni strains with desirable flavor characteristics and to monitor the survival and contribution of inoculated and indigenous bacteria. Therefore, we investigated the suitability of pulsed-field gel electrophoresis (PFGE) for the discrimination of 65 O. oeni isolates from six different wine-producing regions in Germany. Among the restriction enzymes tested, genomic DNA digestions with Sfi I were most effective by displaying 56 (86%) different banding profiles. Our results underline the high capacity of PFGE for strain identification and differentiation. Cluster analysis of the DNA restriction patterns revealed no distinct region specificity of O. oeni populations.
Larisika
Melanie
M
Institute of Microbiology and Wine Research, Johannes Gutenberg-University Mainz, Becherweg 15, D-55099 Mainz, Germany.
Claus
Harald
H
König
Helmut
H
D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005285:Fermentation; D005858:Germany; D006095:Gram-Positive Cocci / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D013045:Species Specificity; D014920:Wine / Q000382:microbiology*
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific