Thermal and photo stability of phenolic constituents of an Achyrocline satureioides spray-dried powder.
Thermal and the photo stabilities of an Achyrocline satureioides powder (SDP40) were evaluated in particular concerning the total polyphenol content as well as the main identified constituents quercetin, luteolin, 3-O-methylquercetin and caffeic acid. SDP40 presented good stability for nine months under normal storage conditions of 25 degrees C temperature and 60% relative humidity (RH). In accelerated term testing, 50 degrees C temperature and 90% RH and also in stress testing, 80 degrees C, caffeic acid and a non-identified constituent P3 were the most instable constituents. Luteolin and 3-O-methylquercetin were the most stable constituents. Quercetin presented an unusual behavior, improving its concentration after 1 month at 50 degrees C or 2 days at 80 degrees C exposition, followed by a decrease in its concentration. The hypothesis that this observation is related to the simultaneous decreasing of a non-identified peak P3 or to the hydrolysis of a non-identified precursor as a quercetin heteroside is being investigated. The SDP40 presented good stability against UV-C light when conditioned in amber or transparent containers, but it suffered degradation when stored in open-dishes. In summary, the total polyphenol content remains within acceptable limits of 10% under normal storage conditions for nine months. However, the LC polyphenol analysis demonstrated that the behavior of individual constituents has still to be enlightened.
Holzschuh
M H
MH
Programa de Pós-graduagdo em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Silva
D M
DM
Schapoval
E E S
EE
Bassani
V L
VL
D036921:Achyrocline / Q000737:chemistry*; D002853:Chromatography, Liquid; D003116:Color; D003890:Desiccation; D004355:Drug Stability; D005419:Flavonoids / Q000737:chemistry* / Q000528:radiation effects; D006358:Hot Temperature; D008027:Light; D010636:Phenols / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry; D059808:Polyphenols; D011208:Powders; D012015:Reference Standards; D012903:Smell; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D011208:Powders