title: Interactions between Oat beta-Glucan and calcofluor characterized by spectroscopic method.
abstract: This paper describes the binding of Calcofluor, a fluorescent probe, to oat beta-glucan in buffer solutions. The binding equilibrium constant (K), the total number of binding sites per beta-glucan molecule (N), and the average binding number of Calcofluor per beta-glucan molecule (n) were determined by UV spectroscopic method. The results indicate that the association of Calcofluor and beta-glucan is driven by both enthalpy and entropy and that the process involves hydrogen bonding, van der Waals forces, and hydrophobic interaction. Higher buffer concentration and NaCl facilitate the binding of Calcofluor to beta-glucan. The adsorption isotherm fits a Langmuir model quite well.
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
authors.forename: Jia
authors.initials: J
authors.lastname: Wu
authors.forename: Xiao
authors.initials: X
authors.lastname: Deng
authors.forename: Binqiang
authors.initials: B
authors.lastname: Tian
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Bijun
authors.initials: B
authors.lastname: Xie
mesh_terms: D000327:Adsorption; D018554:Avena / Q000737:chemistry*; D001557:Benzenesulfonates / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D006860:Hydrogen Bonding; D012965:Sodium Chloride / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D005936:Glucans; D012965:Sodium Chloride; C007061:C.I. Fluorescent Brightening Agent 28