New bioactive clerodane diterpenoids from the roots of Casearia membranacea.
Bioassay-guided fractionation of the acetone extract of the roots of Casearia membranacea furnished three new clerodane diterpenes, caseamembrins S-U (1-3) and the known caseamembrin Q (4). Their structures were established by extensive spectroscopic analyses, especially 2D-NMR. Compounds 1-4 were tested against human tumor cells, including HeLa (cervical epitheloid carcinoma), DLD-1 (colon carcinoma), Daoy (medulloblastoma), and KB (oral epidermoid carcinoma) cell lines. Caseamembrin T (2) exhibited the most potent activity against Daoy cells (ED(50)=10 ng/ml), superior to that of the standard drug mitomycin.
Chen
Ching-Yu
CY
Center of General Education, Tzu-Hui Institute of Technology, Kaohsiung, Taiwan, Republic of China.
Cheng
Yuan-Bin
YB
Chen
Shun-Ying
SY
Chien
Ching-Te
CT
Kuo
Yao-Haur
YH
Guh
Jih-Hwa
JH
Khalil
Ashraf Taha
AT
Shen
Ya-Ching
YC
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D037821:Casearia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045785:Diterpenes, Clerodane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods / Q000592:standards; D008968:Molecular Conformation; D018517:Plant Roots / Q000737:chemistry*; D012015:Reference Standards; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
D000970:Antineoplastic Agents; D045785:Diterpenes, Clerodane