Unsymmetrical DMH - an isomer of 1,2 DMH - is it potent to induce gastrointestinal carcinoma in rats?
Very few animal studies have used 1,1-dimethyl hydrazine (unsymmetrical dimethyl hydrazine - UDMH) as a carcinogen. This study was designed to investigate the carcinogenicity of UDMH in the gastrointestinal tract in a rat model. We wanted to observe if there were any changes in tissue zinc levels and tissue copper zinc superoxide dismutase (CuZnSOD) enzyme activity during the carcinogenic process, and to compare these values with those of control rats in the medium- and long-term. Six-week-old Wistar rats were given a subcutaneous injection of UDMH (30mg/kg body wt) twice a week for 20 weeks, and sacrificed after 5 and 9 months of treatment. Tissue zinc levels showed a significant decrease (p<0.05) in the large intestine at 9 months, whereas in the stomach and small intestine there were no significant changes at 5 and 9 months. Tissue CuZnSOD enzyme activity in the stomach, small intestine and large intestine showed no significant decrease at 5 and 9 months as compared to controls. Histologically, the large intestine was normal at 9 months. This study suggests that UDMH administered at the above dosage was not carcinogenic in this model.
Christudoss
Pamela
P
Department of Clinical Biochemistry, Christian Medical College, Vellore 632004, Tamil Nadu, India. pchristudoss@yahoo.com
Selvakumar
R
R
Pulimood
Anna B
AB
Fleming
J J
JJ
Mathew
George
G
D019813:1,2-Dimethylhydrazine / Q000737:chemistry*; D000818:Animals; D015197:Carcinogenicity Tests; D004127:Dimethylhydrazines / Q000737:chemistry / Q000633:toxicity*; D005753:Gastric Mucosa / Q000378:metabolism; D005770:Gastrointestinal Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D007420:Intestine, Large / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D007536:Isomerism; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000201:enzymology / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D004127:Dimethylhydrazines; C013958:dimazine; D013482:Superoxide Dismutase; D019813:1,2-Dimethylhydrazine; D015032:Zinc