Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection.
A simple, reliable, and reproducible method for separation and determination of five enkephalin-related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8-98.2%.
Wang
Wei
W
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fujian, China.
Yao
Sheyue
S
Qiu
Bin
B
Chen
Guonan
G
D002244:Carbon / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D004745:Enkephalins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D004745:Enkephalins; D010455:Peptides; D002244:Carbon