title: Novel functions of the alpha-ketoglutarate dehydrogenase complex may mediate diverse oxidant-induced changes in mitochondrial enzymes associated with Alzheimer's disease.
abstract: Measures in autopsied brains from Alzheimer's Disease (AD) patients reveal a decrease in the activity of alpha-ketoglutarate dehydrogenase complex (KGDHC) and an increase in malate dehydrogenase (MDH) activity. The present experiments tested whether both changes could be caused by the common oxidant H(2)O(2) and to probe the mechanism underlying these changes. Since the response to H(2)O(2) is modified by the level of the E2k subunit of KGDHC, the interaction of MDH and KGDHC was studied in cells with varying levels of E2k. In cells with only 23% of normal E2k protein levels, one-hour treatment with H(2)O(2) decreased KGDHC and increased MDH activity as well as the mRNA level for both cytosolic and mitochondrial MDH. The increase in MDH did not occur in cells with 100% or 46% of normal E2k. Longer treatments with H(2)O(2) inhibited the activity of both enzymes. Glutathione is a major regulator of cellular redox state and can modify enzyme activities. H(2)O(2) converts reduced glutathione (GSH) to oxidized glutathione (GSSG), which reacts with protein thiols. Treatment of purified KGDHC with GSSG leads to glutathionylation of all three KGDHC subunits. Thus, cellular glutathione level was manipulated by two means to determine the effect on KGDHC and MDH activities. Both buthionine sulfoximine (BSO), which inhibits glutathione synthesis without altering redox state, and H(2)O(2) diminished glutathione to a similar level after 24 h. However, H(2)O(2), but not BSO, reduced KGDHC and MDH activities, and the reduction was greater in the E2k-23 line. These findings suggest that the E2k may mediate diverse responses of KGDHC and MDH to oxidants. In addition, the differential response of activities to BSO and H(2)O(2) together with the in vitro interaction of KGDHC with GSSG suggests that glutathionylation is one possible mechanism underlying oxidative stress-induced inhibition of the TCA cycle enzymes.
authors.affiliation: Department of Neurology and Neuroscience, Weill Medical College of Cornell University/Burke Medical Research Institute, White Plains, New York 10605, USA.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Shi
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Wayne A
authors.initials: WA
authors.lastname: Kleinman
authors.forename: Gary E
authors.initials: GE
authors.lastname: Gibson
mesh_terms: D000544:Alzheimer Disease / Q000201:enzymology*; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002460:Cell Line; D005978:Glutathione / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D007655:Ketoglutarate Dehydrogenase Complex / Q000502:physiology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D024101:Mitochondrial Proteins; D008954:Models, Biological; D018384:Oxidative Stress; D021122:Protein Subunits / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D021122:Protein Subunits; D017382:Reactive Oxygen Species; D019328:Buthionine Sulfoximine; D006861:Hydrogen Peroxide; D008291:Malate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex; D005978:Glutathione