title: [Pulmonary infection induced by Mycobacterium intracellulare in 2 sisters: a biomolecular study of the isolates].
abstract: Patient 1 was an 82-year-old woman without symptoms who had been admitted to the hospital because of abnormal shadows on a chest X ray film. The chest X ray showed a round opacity in the right middle lung field and air-space consolidation in the right lower lung field. A computed tomographic (CT) scan showed a nodular opacity with satellite lesions in the right middle lobe and bronchiectasis in the right middle lobe and left lingular segment. Bronchial lavage yielded Mycobacterium intracellulare. Patient 2 was the 77-year-old sister of Patient 1. She had productive cough and fatigue, and chest X ray films and CT scans showed air-space consolidation with a huge cavity in the left upper lobe. M. intracellulare was isolated repeatedly from sputum samples. Pulsed-field gel electrophoresis disclosed similar large restriction fragment patterns of genomic DNA in 2 strains of the bacterium isolated from both sisters, indicating that the sisters had been infected by genetically close strains. Cross-infection or infection from the same environmental source were suspected.
authors.affiliation: Department of Respiratory Medicine, National Kumamotominami Hospital, 2338 Matubase-mati, Simomasikigun, Kumamoto 869-0524, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimazu
authors.forename: Y
authors.initials: Y
authors.lastname: Iinuma
authors.forename: K
authors.initials: K
authors.lastname: Aman
authors.forename: M
authors.initials: M
authors.lastname: Ebihara
authors.forename: Y
authors.initials: Y
authors.lastname: Nakagawa
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D015269:Mycobacterium avium Complex / Q000235:genetics*; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology* / Q000635:transmission
chemical_list: D004269:DNA, Bacterial