title: Prevalence of 4977bp deletion in mitochondrial DNA from patients with chronic kidney disease receiving conservative treatment or hemodialysis in southern Brazil.
abstract: BACKGROUND
  Damage to mitochondrial DNA (mtDNA) has been described in patients with chronic kidney disease (CKD). The presence of mtDNA 4977bp deletion in many different tissues can serve as a marker of this damage. However, no attempt has been made to detect the presence of mtDNA 4977bp in blood cells of patients with CKD.
  
  
  METHODS
  Polymerase chain reaction techniques (PCR) were used to detect mtDNA 4977bp deletion in blood samples of 94 CKD patients.
  
  
  RESULTS
  The prevalence of 4977bp deletion in mtDNA was 73.1% (38/52) in patients with CKD undergoing hemodialysis, 57.1% (27/42) in patients with CKD receiving conservative treatment, and 27.8% (15/54) in control samples (p < 0.001). Higher prevalence of this mutation was not associated with patient age (p = 0.54) or time on hemodialysis (p = 0.70).
  
  
  CONCLUSIONS
  The higher prevalence of mtDNA 4977bp deletion in patients in this study indicates that the CKD can induce damage to mtDNA in blood cells and could be exacerbated by hemodialysis.
authors.affiliation: Graduate Program in Medical Science, Nephrology and Hemodialysis Service, Clinical Hospital of Porto Alegre, Federal University of Rio Grande do Sul, Porto Alegre, Brazil. liana_rossato@yahoo.com.br
authors.forename: Liana Bertolin
authors.initials: LB
authors.lastname: Rossato
authors.forename: Ane Cláudia Fernandes
authors.initials: AC
authors.lastname: Nunes
authors.forename: Maria Luiza Saraiva
authors.initials: ML
authors.lastname: Pereira
authors.forename: Carolina Fischinger Moura
authors.initials: CF
authors.lastname: de Souza
authors.forename: Claus
authors.initials: C
authors.lastname: Dummer
authors.forename: Vagner
authors.initials: V
authors.lastname: Milani
authors.forename: Daiana Benck
authors.initials: DB
authors.lastname: Porsch
authors.forename: Cristiane Bastos
authors.initials: CB
authors.lastname: de Mattos
authors.forename: Elvino José Guardão
authors.initials: EJ
authors.lastname: Barros
mesh_terms: D000328:Adult; D001483:Base Sequence; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000628:therapy; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D006435:Renal Dialysis; D017384:Sequence Deletion
chemical_list: D004272:DNA, Mitochondrial