title: E4Orf6-E1B-55k-dependent degradation of de novo-generated adeno-associated virus type 5 Rep52 and capsid proteins employs a cullin 5-containing E3 ligase complex.
abstract: Degradation of de novo-generated adeno-associated virus type 5 (AAV5) Rep52 and capsid proteins is part of the limited target specificity displayed by adenovirus type 5 E4Orf6-E1B-55k as part of a cullin 5-containing E3 ligase complex. Both Rep and capsid proteins can be found in the ligase complex, and their presence is dependent on interaction between E4Orf6 and elongins B and C. Degradation of AAV5 proteins can be inhibited by a dominant-negative ubiquitin that prevents chain elongation or by small interfering RNA directed against cullin 5.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine, Life Sciences Center, Columbia, Missouri 65211, USA.
authors.forename: Ramnath
authors.initials: R
authors.lastname: Nayak
authors.forename: K David
authors.initials: KD
authors.lastname: Farris
authors.forename: David J
authors.initials: DJ
authors.lastname: Pintel
mesh_terms: D002460:Cell Line; D044842:Cullin Proteins / Q000378:metabolism*; D000229:Dependovirus / Q000502:physiology*; D006801:Humans; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C496871:CUL5 protein, human; D044842:Cullin Proteins; D014764:Viral Proteins; D044767:Ubiquitin-Protein Ligases