title: Disruption of quinoprotein ethanol dehydrogenase gene and adjacent genes in Pseudomonas putida HK5.
abstract: Pseudomonas putida HK5 produces three different quinoprotein alcohol dehydrogenases: ADH-I, ADH-IIB and ADH-IIG. Gene organization of qedA, the gene for ADH-I, and other 10 genes in the cluster was related to the genome sequences of five other Pseudomonas strains. Insertion mutations in either qedA, exaE or agmR eliminated ADH-I activity, although the mutants were still able to grow on ethanol but more slowly than the wild-type strain. Mutant analysis demonstrated the requirement of agmR and exaE in ADH-I expression, and the tentative involvement of agmR, but not exaE, in the induction of ADH-IIB and ADH-IIG activities.
authors.affiliation: Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Worrawat
authors.initials: W
authors.lastname: Promden
authors.forename: Alisa S
authors.initials: AS
authors.lastname: Vangnai
authors.forename: Piamsook
authors.initials: P
authors.lastname: Pongsawasdi
authors.forename: Osao
authors.initials: O
authors.lastname: Adachi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Hirohide
authors.initials: H
authors.lastname: Toyama
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005798:Genes, Bacterial; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000431:Ethanol; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)