title: Alterations of FHIT and P53 genes in keratocystic odontogenic tumor, dentigerous and radicular cyst.
abstract: BACKGROUND
  The purpose of this study was to determine fragile histidine triad (FHIT) and p53 protein expression, and to analyze FHIT and p53 gene status in keratocystic odontogenic tumor (KOT), dentigerous cysts (DC) and radicular cysts (RC).
  
  
  METHODS
  The methods used were immunohistochemistry and molecular genetic methods including loss of heterozygosity (LOH) and gene sequencing.
  
  
  RESULTS
  FHIT protein expression was different among groups. Aberrant expression was the highest in KOT, then in RC and DC. p53 protein expression was different among groups. LOH in paraffin-embedded specimens was detected in 22.6% and 12.9% for FHIT and p53 respectively. Mutation of p53 gene at codon 237 was observed in only two specimens (one KOT and one DC). Of the six frozen specimens, three exhibited FHIT gene LOH (two RC and one KOT). KOT showed loss of exons 6-7 at FHIT locus and mutation at codon 237 at p53 locus, but this could be a chance result.
  
  
  CONCLUSIONS
  Aberrations of FHIT and p53 genes/proteins could be considered markers responsible for the development of odontogenic lesions.
authors.affiliation: Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia. amalcic@inet.hr
authors.forename: Ana
authors.initials: A
authors.lastname: Malcić
authors.forename: Silvana
authors.initials: S
authors.lastname: Jukić
authors.forename: Ivica
authors.initials: I
authors.lastname: Anić
authors.forename: Bozidar
authors.initials: B
authors.lastname: Pavelić
authors.forename: Sanja
authors.initials: S
authors.lastname: Kapitanović
authors.forename: Bozo
authors.initials: B
authors.lastname: Kruslin
authors.forename: Kresimir
authors.initials: K
authors.lastname: Pavelić
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D017209:Apoptosis; D049109:Cell Proliferation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D003803:Dentigerous Cyst / Q000235:genetics* / Q000378:metabolism; D005260:Female; D016147:Genes, Tumor Suppressor; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007570:Jaw Cysts / Q000235:genetics* / Q000378:metabolism; D007573:Jaw Neoplasms / Q000235:genetics* / Q000378:metabolism; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D009808:Odontogenic Tumors / Q000235:genetics* / Q000378:metabolism; D011842:Radicular Cyst / Q000235:genetics* / Q000378:metabolism; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase