title: Maize centromere mapping: a comparison of physical and genetic strategies.
abstract: Centromere positions on 7 maize chromosomes were compared on the basis of data from 4 to 6 mapping techniques per chromosome. Centromere positions were first located relative to molecular markers by means of radiation hybrid lines and centric fission lines recovered from oat-maize chromosome addition lines. These centromere positions were then compared with new data from centric fission lines recovered from maize plants, half-tetrad mapping, and fluorescence in situ hybridizations and to data from earlier studies. Surprisingly, the choice of mapping technique was not the critical determining factor. Instead, on 4 chromosomes, results from all techniques were consistent with a single centromere position. On chromosomes 1, 3, and 6, centromere positions were not consistent even in studies using the same technique. The conflicting centromere map positions on chromosomes 1, 3, and 6 could be explained by pericentric inversions or alternative centromere positions on these chromosomes.
authors.affiliation: Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, MN 55108-6026, USA. okaga002@umn.edu
authors.forename: Ron J
authors.initials: RJ
authors.lastname: Okagaki
authors.forename: Morrison S
authors.initials: MS
authors.lastname: Jacobs
authors.forename: Adrian O
authors.initials: AO
authors.lastname: Stec
authors.forename: Ralf G
authors.initials: RG
authors.lastname: Kynast
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Buescher
authors.forename: Howard W
authors.initials: HW
authors.lastname: Rines
authors.forename: M Isabel
authors.initials: MI
authors.lastname: Vales
authors.forename: Oscar
authors.initials: O
authors.lastname: Riera-Lizarazu
authors.forename: Martha
authors.initials: M
authors.lastname: Schneerman
authors.forename: Greg
authors.initials: G
authors.lastname: Doyle
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Friedman
authors.forename: Rick W
authors.initials: RW
authors.lastname: Staub
authors.forename: David F
authors.initials: DF
authors.lastname: Weber
authors.forename: Terry L
authors.initials: TL
authors.lastname: Kamps
authors.forename: Ina F E
authors.initials: IF
authors.lastname: Amarillo
authors.forename: Christine D
authors.initials: CD
authors.lastname: Chase
authors.forename: Hank W
authors.initials: HW
authors.lastname: Bass
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Phillips
mesh_terms: D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017404:In Situ Hybridization, Fluorescence; D003313:Zea mays / Q000235:genetics*