title: Plasmid-chromosome shuffling for non-deletion alleles in yeast.
abstract: Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Huang
authors.forename: Richard S
authors.initials: RS
authors.lastname: Sucgang
authors.forename: Yu-yi
authors.initials: YY
authors.lastname: Lin
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Shi
authors.forename: Jef D
authors.initials: JD
authors.lastname: Boeke
authors.forename: Xuewen
authors.initials: X
authors.lastname: Pan
mesh_terms: D000483:Alleles; D032621:DNA Shuffling / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D017353:Gene Deletion; D015723:Gene Library; D020868:Gene Silencing; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal