Developmental potential of isolated blastomeres from early mouse embryos in the presence and absence of LIF and GM-CSF.
OBJECTIVE
The aim of this study was to investigate the developmental potential of isolated blastomeres in the presence and absence of leukemia inhibitory factor (LIF) and granulocyte-macrophage colony stimulating factor (GM-CSF).


METHODS
The blastomeres of two (1/2) and eight cells (1/8) embryos were isolated and cultured in T6 medium in the presence and absence of LIF (1,000 IU/ml) and or GM-CSF (2 ng/ml) up to 120 h. The diameter and cell number of blastocysts were measured.


RESULTS
The developmental rates of 1/2 isolated blastomeres developed to blastocysts stages in the presence and absence of LIF and GM-CSF were 45.80, 35.10 and 48.66, 41.66, respectively. The diameter of blastocysts was higher in GM-CSF group and total cell number of blastocyst in both treated groups was higher than control (P < 0.05). No 1/8 blastomeres developed to morula and blastocyst stages.


CONCLUSIONS
LIF and GM-CSF could improve the development of 1/2 isolated blastomeres.
Sheikholslami
Behnaz
B
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran.
Salehnia
Mojdeh
M
Valojerdi
Mojtaba Rezazadeh
MR
Ramezanzadeh
Mehdi
M
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D001757:Blastomeres / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D008297:Male; D051379:Mice; D011247:Pregnancy
D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D016178:Granulocyte-Macrophage Colony-Stimulating Factor