title: CD33 detection by immunohistochemistry in paraffin-embedded tissues: a new antibody shows excellent specificity and sensitivity for cells of myelomonocytic lineage.
abstract: A new monoclonal antibody to CD33 that reacts in paraffin-embedded tissue samples was evaluated. The expected reactivity in granulocytic and monocytic cells was found in a tissue microarray composed of multiple tissue sites. There was no unexpected reactivity found in a wide variety of hematolymphoid and nonhematolymphoid disorders. In cases of acute leukemia, the CD33 antibody showed equivalent results by immunohistochemical analysis compared with flow cytometric analysis. The CD33 antibody was also found to be a useful marker in the workup of myeloid sarcomas. This anti-CD33 antibody will be a useful marker in the workup of acute leukemias and myeloid sarcomas on paraffin-embedded tissue samples.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: James D
authors.initials: JD
authors.lastname: Hoyer
authors.forename: Karen L
authors.initials: KL
authors.lastname: Grogg
authors.forename: Curtis A
authors.initials: CA
authors.lastname: Hanson
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Gamez
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dogan
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D015415:Biomarkers; D001853:Bone Marrow / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007951:Leukemia, Myeloid / Q000276:immunology; D016612:Paraffin Embedding; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology; D015203:Reproducibility of Results; D023981:Sarcoma, Myeloid / Q000175:diagnosis / Q000276:immunology; D063268:Sialic Acid Binding Ig-like Lectin 3; D046888:Tissue Array Analysis
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3