Generation and characterization of rgs5 mutant mice.
Regulators of G-protein signaling (RGS) are involved in a wide variety of functions, including olfaction, vision, and cell migration. RGS5 has a perivascular expression pattern and was recently identified as a marker for brain pericytes. This suggests a role for RGS5 in vascular development and pericyte biology. We have created a mouse line which lacks the rgs5 gene and replaced it with a green fluorescent protein (GFP) reporter (rgs5(GFP/GFP)). The mice are viable and fertile and display no obvious developmental defects, and the vasculature appears to develop normally with proper pericyte coverage. Also, no differences were observed in the vasculature under pathological conditions, such as tumor growth and oxygen-induced retinopathy. The GFP expression in pericytes of rgs5(GFP) mice allows detection and sorting of these cells, thereby providing a valuable novel tool for pericyte research.
Nisancioglu
Maya H
MH
Karolinska Institutet, Laboratory of Vascular Biology, Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Scheelesväg 2, Plan4:A3, SE-171 77 Stockholm, Sweden.
Mahoney
William M
WM
Kimmel
Dara D
DD
Schwartz
Stephen M
SM
Betsholtz
Christer
C
Genové
Guillem
G
D000818:Animals; D045744:Cell Line, Tumor / Q000637:transplantation; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000235:genetics / Q000502:physiology*; D005260:Female; D005354:Fibrosarcoma / Q000098:blood supply / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006973:Hypertension / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D010100:Oxygen / Q000633:toxicity; D020286:Pericytes / Q000473:pathology; D020710:RGS Proteins / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015861:Retinal Neovascularization / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D012171:Retinal Vessels / Q000187:drug effects / Q000473:pathology
D005136:Eye Proteins; D020710:RGS Proteins; D011993:Recombinant Fusion Proteins; C105346:Rgs5 protein, mouse; D049452:Green Fluorescent Proteins; D010100:Oxygen