Development of a capsid based competitive inhibition enzyme-linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum.
The aim of this study was to develop a more specific and sensitive competitive inhibition ELISA (CI-ELISA) than the currently used indirect ELISA for detection of antibodies to bovine immunodeficiency virus (BIV) in cattle and buffaloes. Murine monoclonal antibodies (MAbs) were generated against a recombinant capsid (CA) protein of bovine immunodeficiency virus. Of the 13 anti-CA MAbs developed, MAb-9G10 was selected for CI-ELISA based on the maximum inhibition (98%) obtained with reference BIV antibody positive serum. Based on the distribution of percent inhibition of known negative sera (n=50), a cut-off value was set at 40% inhibition. The MAb-based CI-ELISA showed much higher agreement (concordance: 95.4%) than the indirect ELISA (concordance: 77.8%) with Western blot. Out of 672 sera of cattle and buffaloes tested by CI-ELISA from four states of India, 22% (113/516) of cattle and 19% (30/156) of buffalo were sero-positive for BIV with an overall seroprevalence of 21% (143/672) in India.
Bhatia
S
S
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anandnagar, Bhopal, Madhya Pradesh 462021, India. bhatia_san@yahoo.co.in <bhatia_san@yahoo.co.in>
Sood
Richa
R
Bhatia
A K
AK
Pattnaik
B
B
Pradhan
H K
HK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D016087:Immunodeficiency Virus, Bovine / Q000276:immunology / Q000302:isolation & purification*; D007194:India; D016180:Lentivirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D051379:Mice; D016036:Seroepidemiologic Studies
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral