Ym1 and Ym2 expression in a mouse model exposed to diesel exhaust particles.
BACKGROUND
Chitinase may play a role in regulating allergic diseases.


OBJECTIVE
We studied the role of chitinase in a mouse model exposed to diesel exhaust particles (DEP). Mice were exposed to intranasal DEP (0.6 mg/mL) for 5 days and challenged with aerosolized DEP (6 mg/m(3)) on days 6-8. Enhanced pause (Penh), as an airway obstruction marker, was measured on day 9, and bronchoalveolar lavage (BAL) fluid and lung tissues were collected on day 10. The expression of Ym1 and Ym2 mRNA was assessed in lung tissue extracts by reverse transcription-polymerase chain reaction.


RESULTS
DEP induced significant increases in methacholine-induced Penh and IL-4 levels in BAL fluid relative to the control group. Peribronchial and perivascular inflammatory cell infiltrates were prominent in the DEP group. DEP induced Ym1 and Ym2 mRNA expression in lung tissue extracts relative to the control group.


CONCLUSIONS
These results demonstrate that DEP induced airway hyperresponsiveness and Ym mRNA expression via a Th2 cell-biased response, suggesting that chitinase may play an important role in airway inflammation and responsiveness upon exposure to DEP in a mouse model, and may therefore be involved in regulating allergic diseases.
Song
Hyun-Mi
HM
Department of Internal Medicine, Soonchunhyang University Hospital, Bucheon, Korea.
Jang
An-Soo
AS
Ahn
Mi-Hyun
MH
Takizawa
Hajime
H
Lee
Shin-Hwa
SH
Kwon
Ji-Hee
JH
Lee
Young-Mok
YM
Rhim
Tai Youn
TY
Park
Choon-Sik
CS
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002688:Chitinases / Q000235:genetics; D004195:Disease Models, Animal; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D005742:Gasoline / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D019570:Inhalation Exposure; D015847:Interleukin-4 / Q000378:metabolism; D037102:Lectins / Q000235:genetics; D016210:Methacholine Chloride; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D001335:Vehicle Emissions / Q000633:toxicity; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics
D000393:Air Pollutants; D005742:Gasoline; D037102:Lectins; D012333:RNA, Messenger; D001335:Vehicle Emissions; D016210:Methacholine Chloride; D015847:Interleukin-4; D002688:Chitinases; C525260:Ym2 protein, mouse; C429541:Chil3 protein, mouse; D001619:beta-N-Acetylhexosaminidases