title: CD157 is part of a supramolecular complex with CD11b/CD18 on the human neutrophil cell surface.
abstract: CD157 is a GPI-anchored cell surface glycoprotein expressed by human peripheral blood neutrophils. Cross-linking of CD157 induces intracellular Ca2+ mobilization and re-shaping in neutrophils, thus regulating their adhesive and migratory properties. Results obtained by immunolocalization and confocal microscopy indicate that CD157 lies in close proximity to the CD11b/CD18 complex which is strongly expressed on the activated neutrophil cell membrane where it plays a predominant role in adhesion. This study analyses the physical association between CD157 and CD18 in human neutrophils by co-immunoprecipitation experiments. The anti-CD157 monoclonal antibody RF3 co-precipitates CD18, and the anti-CD18 antibody TS1/18 co-precipitates CD157 from human neutrophil lysates. These results confirm that CD157 physically interacts with CD11b/CD18 complex in human neutrophils.
authors.affiliation: Laboratory of Immunogenetics, Department of Genetics, Biology and Biochemistry, University of Torino, Torino, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lavagno
authors.forename: E
authors.initials: E
authors.lastname: Ferrero
authors.forename: E
authors.initials: E
authors.lastname: Ortolan
authors.forename: F
authors.initials: F
authors.lastname: Malavasi
authors.forename: A
authors.initials: A
authors.lastname: Funaro
mesh_terms: D036541:ADP-ribosyl Cyclase / Q000276:immunology / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D039481:CD11b Antigen / Q000276:immunology / Q000378:metabolism*; D018821:CD18 Antigens / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D006801:Humans; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D009504:Neutrophils / Q000378:metabolism*; D025941:Protein Interaction Mapping
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039481:CD11b Antigen; D018821:CD18 Antigens; D058851:GPI-Linked Proteins; C509295:ITGAM protein, human; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2