title: Outer membrane modifications of Pseudomonas fluorescens MF37 in response to hyperosmolarity.
abstract: The effect of hyperosmotic condition on the outer membrane protein (Omp) composition of Pseudomonas fluorescens was investigated by proteomic analyses. The abundances of 12 proteins, including porins, lipoproteins, and the flagella subunit FliC, were modified. This was at least partly explained by altered gene expression, as shown by mRNA level study. In agreement with Omp changes, hyperosmotic condition resulted in vesicle formation and modifications of mobility and antibiotic susceptibility.
authors.affiliation: Laboratoire de Microbiologie du Froid, UPRES EA 2123, Université de Rouen, Evreux, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Guyard-Nicodème
authors.forename: Alexis
authors.initials: A
authors.lastname: Bazire
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Hémery
authors.forename: Thierry
authors.initials: T
authors.lastname: Meylheuc
authors.forename: Daniel
authors.initials: D
authors.lastname: Mollé
authors.forename: Nicole
authors.initials: N
authors.lastname: Orange
authors.forename: Laurène
authors.initials: L
authors.lastname: Fito-Boncompte
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.forename: Dominique
authors.initials: D
authors.lastname: Haras
authors.forename: Alain
authors.initials: A
authors.lastname: Dufour
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevalier
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001425:Bacterial Outer Membrane Proteins; D012333:RNA, Messenger