title: Evolutionary conserved N-terminal region of human muscle fructose 1,6-bisphosphatase regulates its activity and the interaction with aldolase.
abstract: N-terminal residues of muscle fructose 1,6-bisphosphatase (FBPase) are highly conserved among vertebrates. In this article, we present evidence that the conservation is responsible for the unique properties of the muscle FBPase isozyme: high sensitivity to AMP and Ca(2+) inhibition and the high affinity to muscle aldolase, which is a factor desensitizing muscle FBPase toward AMP and Ca(2+). The first N-terminal residue affecting the affinity of muscle FBPase to aldolase is arginine 3. On the other hand, the first residue significantly influencing the kinetics of muscle FBPase is proline 5. Truncation from 5-7 N-terminal residues of the enzyme not only decreases its affinity to aldolase but also reduces its k-(cat) and activation by Mg(2+), and desensitizes FBPase to inhibition by AMP and calcium ions. Deletion of the first 10 amino acids of muscle FBPase abolishes cooperativity of Mg(2+) activation and results in biphasic inhibition of the enzyme by AMP. Moreover, this truncation lowers affinity of muscle FBPase to aldolase about 14 times, making it resemble the liver isozyme. We suggest that the existence of highly AMP-sensitive muscle-like FBPase, activity of which is regulated by metabolite-dependent interaction with aldolase enables the precise regulation of muscle energy expenditures and might contributed to the evolutionary success of vertebrates.
authors.affiliation: Department of Animal Physiology, Institute of Zoology, Wroclaw University, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gizak
authors.forename: Ewa
authors.initials: E
authors.lastname: Maciaszczyk
authors.forename: Andrzej
authors.initials: A
authors.lastname: Dzugaj
authors.forename: Klaus
authors.initials: K
authors.lastname: Eschrich
authors.forename: Darek
authors.initials: D
authors.lastname: Rakus
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000494:pharmacology; D017124:Conserved Sequence; D019143:Evolution, Molecular; D006597:Fructose-Bisphosphatase / Q000737:chemistry* / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008969:Molecular Sequence Data; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D050505:Mutant Proteins / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D012235:Rhodamines / Q000378:metabolism; D012518:Sarcomeres / Q000187:drug effects / Q000201:enzymology; D013329:Structure-Activity Relationship
chemical_list: D050505:Mutant Proteins; D012235:Rhodamines; C009434:tetramethylrhodamine isothiocyanate; D006597:Fructose-Bisphosphatase; D005634:Fructose-Bisphosphate Aldolase; D008274:Magnesium; D002118:Calcium