title: First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association.
abstract: We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
authors.affiliation: Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brasil.
authors.forename: Desiely Silva
authors.initials: DS
authors.lastname: Gusmão
authors.forename: Adão Valmir
authors.initials: AV
authors.lastname: Santos
authors.forename: Danyelle Cristine
authors.initials: DC
authors.lastname: Marini
authors.forename: Erica de Souza
authors.initials: Ede S
authors.lastname: Russo
authors.forename: Anelise Maria Dias
authors.initials: AM
authors.lastname: Peixoto
authors.forename: Maurício
authors.initials: M
authors.lastname: Bacci Júnior
authors.forename: Marília Amorim
authors.initials: MA
authors.lastname: Berbert-Molina
authors.forename: Francisco José Alves
authors.initials: FJ
authors.lastname: Lemos
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001407:Bacillus / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology*; D010843:Pichia / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012705:Serratia / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012339:RNA, Ribosomal, 28S