The effects of local selection versus dispersal on insecticide resistance patterns: longitudinal evidence from diamondback moth (Plutella xylostella (Lepidoptera: Plutellidae)) in Australia evolving resistance to pyrethroids.
When strong directional selection acts on a trait, the spatial distribution of phenotypes may reflect effects of selection, as well as the spread of favoured genotypes by gene flow. Here we investigate the relative impact of these factors by assessing resistance to synthetic pyrethroids in a 12-year study of diamondback moth, Plutella xylostella, from southern Australia. We estimated resistance levels in populations from brassicaceous weeds, canola, forage crops and vegetables. Differences in resistance among local populations sampled repeatedly were stable over several years. Levels were lowest in samples from weeds and highest in vegetables. Resistance in canola samples increased over time as insecticide use increased. There was no evidence that selection in one area influenced resistance in adjacent areas. Microsatellite variation from 13 populations showed a low level of genetic variation among populations, with an AMOVA indicating that population only accounted for 0.25% of the molecular variation. This compared to an estimate of 13.8% of variation accounted for by the resistance trait. Results suggest that local selection rather than gene flow of resistance alleles dictated variation in resistance across populations. Therefore, regional resistance management strategies may not limit resistance evolution.
Endersby
N M
NM
Centre for Environmental Stress and Adaptation Research, School of Biological Sciences, Monash University, VIC, Australia. nancye@unimelb.edu.au
Ridland
P M
PM
Hoffmann
A A
AA
D000704:Analysis of Variance; D000818:Animals; D001315:Australia; D003710:Demography; D019143:Evolution, Molecular; D014644:Genetic Variation; D005828:Genetics, Population; D007305:Insecticide Resistance / Q000235:genetics*; D008137:Longitudinal Studies; D018895:Microsatellite Repeats / Q000235:genetics; D009036:Moths / Q000187:drug effects* / Q000235:genetics*; D011722:Pyrethrins / Q000633:toxicity*; D012641:Selection, Genetic
D011722:Pyrethrins