title: Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients.
abstract: We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.
authors.affiliation: Bundeswehr Institute of Microbiology, Munich, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Wölfel
authors.forename: Janusz T
authors.initials: JT
authors.lastname: Paweska
authors.forename: Nadine
authors.initials: N
authors.lastname: Petersen
authors.forename: Antoinette A
authors.initials: AA
authors.lastname: Grobbelaar
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Leman
authors.forename: Roger
authors.initials: R
authors.lastname: Hewson
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Georges-Courbot
authors.forename: Anna
authors.initials: A
authors.lastname: Papa
authors.forename: Stephan
authors.initials: S
authors.lastname: Günther
authors.forename: Christian
authors.initials: C
authors.lastname: Drosten
mesh_terms: D014644:Genetic Variation; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006479:Hemorrhagic Fever, Crimean / Q000097:blood / Q000175:diagnosis* / Q000401:mortality; D006801:Humans; D010802:Phylogeny; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D019562:Viral Load