Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds.
DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity in digestion has not yet been verified. Development allows for advances in site specific localization of components using biological media.
Slavin
John W J
JW
Department of Chemistry , Purdue University, West Lafayette, IN 47907, USA.
Ivanisevic
Albena
A
D002412:Cations; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties
D002412:Cations; D020099:Coated Materials, Biocompatible; D006046:Gold; D004247:DNA; D004262:DNA Restriction Enzymes