title: Influence of antisperm antibodies in the semen on intracytoplasmic sperm injection outcome.
abstract: OBJECTIVE
  The aim of this study was to analyze the influence of autoantibodies against spermatozoa present in the semen on the outcome of in vitro fertilization with intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  We performed a retrospective analysis of clinical and laboratorial data from a six year-period ICSI cycles. Screening for the presence of ASA in the semen, by using the direct immunobeads test (IBT), was available for 351 cycles. According to the percentage of antibody-bound spermatozoa in the semen, we divided the cycles in four groups: I (n = 194): 0%-10% ASA; II (n = 107): 11%-20%; III (n = 33): 21%-50% and IV (n = 17): 51%-100% ASA. Additionally, a group of 349 ICSI cycles performed with ejaculated spermatozoa from oligo/asthenozoospermic men who had insufficient number of motile sperm available for ASA screening was included for comparison. ICSI outcomes were compared among groups and included fertilization rate (2 PN), cleavage rate, cleavage velocity, embryo quality, clinical pregnancy and miscarriage rates. Data were examined statistically, with an alpha level of 5% considered significant.
  
  
  RESULTS
  Fertilization, cleavage rate and velocity, percentage of good quality embryos, as well as clinical pregnancy and miscarriage rates did not differ among different ASA levels groups. ICSI outcomes in men exhibiting different levels of autoimmunity against spermatozoa did not differ from those with severely abnormal seminal parameters.
  
  
  CONCLUSIONS
  Our data indicate that intracytoplasmic sperm injection (ICSI) outcomes are not influenced by ASA levels on sperm.
authors.affiliation: Androfert, Male Reproduction Reference Center, Av. Dr. Heitor Penteado 1464, Campinas, Sao Paulo, Brazil. e.esteves@androfert.com.br
authors.forename: Sandro C
authors.initials: SC
authors.lastname: Esteves
authors.forename: Danielle T
authors.initials: DT
authors.lastname: Schneider
authors.forename: Sidney
authors.initials: S
authors.lastname: Verza
mesh_terms: D000704:Analysis of Variance; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood; D016009:Chi-Square Distribution; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000276:immunology / Q000628:therapy*; D008297:Male; D009865:Oocytes / Q000276:immunology; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D012661:Semen / Q000276:immunology*; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D000906:Antibodies; D001323:Autoantibodies