Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esterase.
Bartsch
Sebastian
S
Institute of Biochemistry, Deparment of Biotechnology & Enzyme Catalysis, Greifswald University, Felix-Hausdorff-Strasse 4, 17487 Greifswald, Germany.
Kourist
Robert
R
Bornscheuer
Uwe T
UT
D000085:Acetates / Q000737:chemistry; D000107:Acetylation; D000438:Alcohols / Q000737:chemistry* / Q000378:metabolism*; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites; D004950:Esterases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics*; D013237:Stereoisomerism
D000085:Acetates; D000438:Alcohols; D004950:Esterases