title: Adoptively transferred dendritic cells restore primary cell-mediated inflammatory competence to acutely malnourished weanling mice.
abstract: Immune depression associated with prepubescent malnutrition underlies a staggering burden of infection-related morbidity. This investigation centered on dendritic cells as potentially decisive in this phenomenon. C57BL/6J mice, initially 19 days old, had free access for 14 days to a complete diet or to a low-protein formulation that induced wasting deficits of protein and energy. Mice were sensitized by i.p. injection of sheep red blood cells on day 9, at which time one-half of the animals in each dietary group received a simultaneous injection of 10(6) syngeneic dendritic cells (JAWS II). All mice were challenged with the immunizing antigen in the right hind footpad on day 13, and the 24-hour delayed hypersensitivity response was assessed as percentage increase in footpad thickness. The low-protein diet reduced the inflammatory immune response, but JAWS cells, which exhibited immature phenotypic and functional characteristics, increased the response of both the malnourished group and the controls. By contrast, i.p. injection of 10(6) syngeneic T cells did not influence the inflammatory immune response of mice subjected to the low-protein protocol. Antigen-presenting cell numbers limited primary inflammatory cell-mediated competence in this model of wasting malnutrition, an outcome that challenges the prevailing multifactorial model of malnutrition-associated immune depression. Thus, a new dendritic cell-centered perspective emerges regarding the cellular mechanism underlying immune depression in acute pediatric protein and energy deficit.
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, ON, Canada N1G 2W1.
authors.forename: Lyn
authors.initials: L
authors.lastname: Hillyer
authors.forename: Charlene
authors.initials: C
authors.lastname: Whitley
authors.forename: Amy
authors.initials: A
authors.lastname: Olver
authors.forename: Michelle
authors.initials: M
authors.lastname: Webster
authors.forename: Tessa
authors.initials: T
authors.lastname: Steevels
authors.forename: Bill
authors.initials: B
authors.lastname: Woodward
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D005260:Female; D005434:Flow Cytometry; D007111:Immunity, Cellular; D007249:Inflammation / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011502:Protein-Energy Malnutrition / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation; D019282:Wasting Syndrome / Q000209:etiology; D014886:Weaning