Protein recording material: photorecord/erasable protein array using a UV-eliminative linker.
Protein patterning on solid surfaces is a topic of significant importance in the fields of biosensors, diagnostic assays, cell adhesion technologies, and biochip microarrays. In this letter, we have established a novel, rapid method for the fabrication of a "protein recording material", which enables us to spatiotemporally regulate the recording, reading, and erasing of a fluorescent protein array as information by a photochemical technique. A photolinker that we synthesized here was used to control the protein array spatiotemporally. The recording process was almost completed after 1 min of photoirradiation to read a clear pattern consisting of a specific protein-ligand complex with high spatiotemporal resolution. The erasing of the protein array was then achieved by photoirradiation onto the entire patterned surface.
Nakayama
Koji
K
The Institute of Scientific and Industrial Research (ISIR), Osaka University Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
Tachikawa
Takashi
T
Majima
Tetsuro
T
D003432:Cross-Linking Reagents / Q000737:chemistry*; D016247:Information Storage and Retrieval / Q000379:methods*; D015394:Molecular Structure; D010777:Photochemistry; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D011506:Proteins