Comparison of the efficacy of two commercially available media for culturing one-cell embryos in the in vitro fertilization mouse model.
OBJECTIVE
To examine the effects of two commercial media on the development of mouse ova fertilized in vitro to the blastocyst stage.


METHODS
Animal model.


METHODS
Academic institution.


METHODS
Eight-week old, superovulated mice.


METHODS
One-cell embryos cultured in vitro up to the blastocyst stage in potassium-enriched simplex optimized medium (KSOM) or G1/G2 medium.


METHODS
Blastocyst and hatching rates, total cell number count, and proportion of allocation of cells to the inner cell mass (ICM) and trophectoderm (TE).


RESULTS
The percentage of zygotes that developed to the blastocyst stage 96 and 120 hours after insemination was statistically significantly higher in the KSOM group. The percentage of blastocysts that partially or completely hatched by day 5 of culture was 84% and 71% for the KSOM and G1/G2 groups, respectively, showing a statistically significant difference between the groups. The mean number of ICM cells was 11.7 +/- 4.0 and 9.2 +/- 5.2 for the zygotes cultured in KSOM and G1/G2 media, respectively, revealing a statistically significantly higher cell number in the ICM of blastocysts derived from culture in KSOM medium. The ICM/TE ratio in the blastocysts cultured in KSOM or G1/G2 media was similar in both groups.


CONCLUSIONS
Commercially available KSOM medium is superior to sequential G1/G2 media for culturing one-cell embryos up to the blastocyst stage in the mouse IVF model.
Perin
Paulo Marcelo
PM
Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil. paulo@ceerh.com.br
Maluf
Mariangela
M
Nicolosi Foltran Januário
Daniela Aparecida
DA
Nascimento Saldiva
Paulo Hilário
PH
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D051379:Mice; D015053:Zygote / Q000166:cytology / Q000254:growth & development
D003470:Culture Media