title: High-density mapping of single-molecule trajectories with photoactivated localization microscopy.
abstract: We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.
authors.affiliation: National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Suliana
authors.initials: S
authors.lastname: Manley
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Gillette
authors.forename: George H
authors.initials: GH
authors.lastname: Patterson
authors.forename: Hari
authors.initials: H
authors.lastname: Shroff
authors.forename: Harald F
authors.initials: HF
authors.lastname: Hess
authors.forename: Eric
authors.initials: E
authors.lastname: Betzig
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lippincott-Schwartz
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D007089:Image Enhancement / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence / Q000379:methods*
chemical_list: D008565:Membrane Proteins