Maize centromere mapping: a comparison of physical and genetic strategies.
Centromere positions on 7 maize chromosomes were compared on the basis of data from 4 to 6 mapping techniques per chromosome. Centromere positions were first located relative to molecular markers by means of radiation hybrid lines and centric fission lines recovered from oat-maize chromosome addition lines. These centromere positions were then compared with new data from centric fission lines recovered from maize plants, half-tetrad mapping, and fluorescence in situ hybridizations and to data from earlier studies. Surprisingly, the choice of mapping technique was not the critical determining factor. Instead, on 4 chromosomes, results from all techniques were consistent with a single centromere position. On chromosomes 1, 3, and 6, centromere positions were not consistent even in studies using the same technique. The conflicting centromere map positions on chromosomes 1, 3, and 6 could be explained by pericentric inversions or alternative centromere positions on these chromosomes.
Okagaki
Ron J
RJ
Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, MN 55108-6026, USA. okaga002@umn.edu
Jacobs
Morrison S
MS
Stec
Adrian O
AO
Kynast
Ralf G
RG
Buescher
Elizabeth
E
Rines
Howard W
HW
Vales
M Isabel
MI
Riera-Lizarazu
Oscar
O
Schneerman
Martha
M
Doyle
Greg
G
Friedman
Katherine L
KL
Staub
Rick W
RW
Weber
David F
DF
Kamps
Terry L
TL
Amarillo
Ina F E
IF
Chase
Christine D
CD
Bass
Hank W
HW
Phillips
Ronald L
RL
D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D017404:In Situ Hybridization, Fluorescence; D003313:Zea mays / Q000235:genetics*