Chromium(III) triggers the DNA-damaged checkpoint of the cell cycle and induces a functional increase of 4E-BP.
Using sea urchin early embryos as a pertinent model, chromium(III) provoked cell cycle arrest and induced apoptosis. The molecular machinery of translation initiation was investigated. Chromium provoked a time- and dose-dependent increase in the level of 4E-BP protein, the natural regulator of the cap-dependent initiation factor 4E (eIF4E). The 4E-BP increase was the result of 4E-BP stabilization and appeared functional for physiological eIF4E binding, removal of eIF4E from the initiation factor eIF4G, and almost full inhibition of cap-dependent translation in vivo. The protein 4E-BP may be involved in the biological pathway of apoptosis associated with the activation of the DNA-damaged checkpoint of the cell cycle.
Le Bouffant
Ronan
R
UPMC Univ Paris 06, UMR 7150, Mer & Santé, Station Biologique, F-29680, Roscoff, France.
Mulner-Lorillon
Odile
O
Morales
Julia
J
Cormier
Patrick
P
Bellé
Robert
R
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects* / Q000235:genetics; D002712:Chlorides / Q000633:toxicity*; D017608:Chromium Compounds / Q000633:toxicity*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects; D039561:Eukaryotic Initiation Factor-4E / Q000032:analysis / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D010063:Ovum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D011485:Protein Binding; D012617:Sea Urchins / Q000196:embryology / Q000502:physiology; D013997:Time Factors
D002712:Chlorides; D017608:Chromium Compounds; D039561:Eukaryotic Initiation Factor-4E; D047908:Intracellular Signaling Peptides and Proteins; C017133:chromous chloride