A transient proteasome activation is needed for acetic acid-induced programmed cell death to occur in Saccharomyces cerevisiae.
To gain further insight into the mechanism by which yeast programmed cell death (PCD) occurs, we investigated whether and how proteasome activity changes in Saccharomyces cerevisiae cells undergoing PCD as a result of treatment with acetic acid (AA-PCD). We show that proteasome activation starts 60 min after AA-PCD induction, with a maximum at 90 min, and decreases at 150 min. Moreover, cell survival measurements carried out in the absence or presence of MG132, which inhibits proteasome function, show that the inhibition of proteasome activity partially prevents AA-PCD, thus indicating that a transient proteasome activation is needed for AA-PCD to occur.
Valenti
Daniela
D
Istituto di Biomembrane e Bioenergetica, Consiglio Nazionale delle Ricerche, Bari, Italy.
Vacca
Rosa Anna
RA
Guaragnella
Nicoletta
N
Passarella
Salvatore
S
Marra
Ersilia
E
Giannattasio
Sergio
S
D019342:Acetic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004789:Enzyme Activation; D046988:Proteasome Endopeptidase Complex / Q000502:physiology*; D061988:Proteasome Inhibitors; D012441:Saccharomyces cerevisiae / Q000166:cytology*
D061988:Proteasome Inhibitors; D046988:Proteasome Endopeptidase Complex; D019342:Acetic Acid