Plasmid-chromosome shuffling for non-deletion alleles in yeast.
Here we describe a facile plasmid-chromosome shuffling technique for generating and analyzing non-deletion alleles in the yeast Saccharomyces cerevisiae. This technique takes advantage of an existing set of genome-wide haploid-convertible heterozygous diploid yeast knockout mutants. This simple method will facilitate characterization of essential gene functions and genome-wide investigation of protein structure-function relationships.
Huang
Zhiwei
Z
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
Sucgang
Richard S
RS
Lin
Yu-yi
YY
Shi
Xiaomin
X
Boeke
Jef D
JD
Pan
Xuewen
X
D000483:Alleles; D032621:DNA Shuffling / Q000379:methods*; D004271:DNA, Fungal / Q000235:genetics*; D017353:Gene Deletion; D015723:Gene Library; D020868:Gene Silencing; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal