Recognition of Staphylococcus enterotoxin via molecularly imprinted beads.
The synthesis of molecularly imprinted beads for the recognition of the protein Staphylococcus enterotoxin B (SEB) is described. Two kinds of organic silane (3-aminopropyltrimethoxysilane (APTMS) and octyltrimethoxysilane (OTMS)) were polymerized on the surface of polystyrene microspheres after the SEB template was covalently immobilized by forming imine bonds. The resulting imprinted beads were selective for SEB. The Langmuir adsorption models were applied to describe the equilibrium isotherms. The results showed that an equal class of adsorption was formed in the molecularly imprinted polymer (MIP) with the maximum adsorption capacity of 3.86 mg SEB/g imprinted beads. The MIP has much higher adsorption capacity for SEB than the nonimprinted polymer, and the MIP beads have a higher selectivity for the template molecule.
Yao
Wei
W
Institute of Hygienic and Environmental Medicine, Tianjin, PR China.
Ning
Baoan
B
Zhou
Huanying
H
Fang
Yanjun
Y
Gao
Zhixian
Z
D000327:Adsorption; D001427:Bacterial Toxins / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000737:chemistry*; D013816:Thermodynamics
D001427:Bacterial Toxins; D004768:Enterotoxins; D011108:Polymers