title: Effects of ammonium tetrathiomolybdate, an oncolytic/angiolytic drug on the viability and proliferation of endothelial and tumor cells.
abstract: OBJECTIVE
  This study examines the target specificity of tetrathiomolybdate (ATM), an anti-angiogenic, anti-tumor agent against the viability / proliferation of arterial, venous, capillary endothelial and tumor cells.
  
  
  METHODS
  Cells were seeded at 10 or 100% density (to measure viability / proliferation respectively) in medium +/- 0-250 uM ATM. Viability and proliferation were measured by metabolic labeling-colorimetry. One-way ANOVA + Bonferroni testing examined base metabolism; Dunnett's testing was used for viability/proliferation.
  
  
  RESULTS
  Venous proliferation showed high ATM sensitivity (50% reduction ~ > or =5 uM ATM, p<0.01), capillary proliferation was inhibited > or =10 uM (p<0.05). Arterial endothelium were less sensitive to ATM, (50% inhibition ~ > or = 20 uM, p<0.01). YPEN-1 were inhibited >50 uM ATM. Capillary viability was inhibited > or =20 microM ATM (p<0.01); venous, arterial and tumor viability show less ATM sensitivity.
  
  
  CONCLUSIONS
  Our data suggest that venous and capillary endothelial proliferation are important targets in ATM therapy, but that other vascular segments and tumor cells may be less influenced.
authors.affiliation: Department of Molecular and Cellular Physiology, LSU Health Sciences Center, Shreveport, LA 71130-3932, USA.
authors.forename: A
authors.initials: A
authors.lastname: Carpenter
authors.forename: A
authors.initials: A
authors.lastname: Rassam
authors.forename: M H
authors.initials: MH
authors.lastname: Jennings
authors.forename: S
authors.initials: S
authors.lastname: Robinson-Jackson
authors.forename: J S
authors.initials: JS
authors.lastname: Alexander
authors.forename: C
authors.initials: C
authors.lastname: Erkuran-Yilmaz
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008982:Molybdenum / Q000494:pharmacology*; D051381:Rats
chemical_list: D020533:Angiogenesis Inhibitors; D008982:Molybdenum; C020809:tetrathiomolybdate