Effects of H2O2 under low- and high-aeration-level conditions on growth and catalase activity in Exiguobacterium oxidotolerans T-2-2T.
The effects of H2O2 under low- and high-aeration-level conditions on growth and catalase activity in Exiguobacterium oxidotolerans T-2-2T were investigated. Continuous addition of 5-200 mM H2O2 to the culture medium from the mid-exponential growth phase enhanced the growth of the strain under the low-aeration-level condition, whereas the addition of 5-50 mM H2O2 decreased intracellular specific catalase activity and extracellular total catalases activity. The detection of extracellular catalase by the cells and the decrease in intracellular specific catalase activity and extracellular total catalase activity under the high-aeration-level condition account for the stimulation of growth by the introduced H2O2 and the decrease in catalase activities induced by O(2) from H2O2 in the medium. On the other hand, the addition of H2O2 to the medium prior to the initiation of growth inhibited the growth but increased the specific activity of intracellular catalase in the stationary growth phase. Strain T-2-2T grew when 10 mM H2O2 was added to the medium prior to growth. However, the growth was completely inhibited by the catalase inhibitor 3-amino-1,2,4-triazole (3-AT). The continuous addition of H2O2 at an appropriate concentration from prior to the initiation of growth to the stationary growth phase under the low-aeration-level condition resulted in higher intracellular specific catalase activity and cell growth rate than single H2O2 addition prior to growth.
Takebe
Fumihiko
F
Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Hara
Isao
I
Matsuyama
Hidetoshi
H
Yumoto
Isao
I
D001406:Bacillaceae / Q000201:enzymology* / Q000254:growth & development*; D002374:Catalase / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D010100:Oxygen / Q000378:metabolism*
D006861:Hydrogen Peroxide; D002374:Catalase; D010100:Oxygen