title: Conformational and quantitative characterization of lysozyme extracted from galyfilcon and senofilcon silicone hydrogel contact lenses.
abstract: OBJECTIVE
  To compare two solvents for retrieval of lysozyme deposited on a silicone hydrogel (SH) contact lens material galyfilcon A (GA; Acuvue Advance).
  
  
  METHODS
  Two buffers used were 50:50 acetonitrile/0.02% trifluoroacetic acid (buffer 1) and 50:50 acetonitrile/50 mM NaOH (buffer 2).
  
  
  RESULTS
  Extraction efficiency from GA lenses was 74% (buffer 1) and 83% (buffer 2). Buffer 2 decreased lysozyme activity > buffer 1. Ex vivo GA lenses showed total protein deposition of 2-16 microg/lens with total lysozyme deposition of 0.3-3.9 microg/lens.
  
  
  CONCLUSIONS
  We have developed a low acid strength extraction buffer that can be used to efficiently extract active lysozyme protein from novel siloxane-based contact lens materials.
authors.affiliation: Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Mary-Ann
authors.initials: MA
authors.lastname: Glasier
authors.forename: Adam
authors.initials: A
authors.lastname: Keech
authors.forename: Heather
authors.initials: H
authors.lastname: Sheardown
authors.forename: Lakshman N
authors.initials: LN
authors.lastname: Subbaraman
authors.forename: Lyndon
authors.initials: L
authors.lastname: Jones
mesh_terms: D000327:Adsorption; D015153:Blotting, Western; D002021:Buffers; D003263:Contact Lenses, Hydrophilic; D006801:Humans; D020100:Hydrogels; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification*; D012828:Silicones; D012997:Solvents
chemical_list: D002021:Buffers; D020100:Hydrogels; D012828:Silicones; D012997:Solvents; D009113:Muramidase