title: Yeast and mammalian two-hybrid systems for studying protein-protein interactions.
abstract: An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.
authors.affiliation: Burnham Institute for Medical Research, La Jolla, CA, USA.
authors.forename: Shu-ichi
authors.initials: S
authors.lastname: Matsuzawa
authors.forename: John C
authors.initials: JC
authors.lastname: Reed
mesh_terms: D000818:Animals; D006801:Humans; D007763:Lac Operon; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D020798:Two-Hybrid System Techniques
chemical_list: D011506:Proteins