title: Purification, crystallization and preliminary X-ray studies of AxCesD required for efficient cellulose biosynthesis in Acetobacter xylinum.
abstract: AxCesD protein required for bacterial cellulose biosynthesis in Acetobacter xylinum was overexpressed in E. coli, purified and crystallized. Single crystals of SeMet-substituted AxCesD were obtained by the sitting-drop vapor-diffusion method. The crystal belongs to the primitive trigonal space group P3 2, with unit-cell parameters a = b = 77.7 A, and c = 213.9 A. The asymmetric unit in the crystal was assumed to contain 8 protein molecules giving the Matthews coefficient (VM) of 2.54 A3 Da(-1). Se-MAD data were collected to 2.3 A resolution using synchrotron radiations.
authors.affiliation: Faculty of Advanced Life Science, Hokkaido University, 060-0810, Sapporo, Japan.
authors.forename: SongQing
authors.initials: S
authors.lastname: Hu
authors.forename: YongGui
authors.initials: Y
authors.lastname: Gao
authors.forename: Kenji
authors.initials: K
authors.lastname: Tajima
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Takanori
authors.initials: T
authors.lastname: Yoda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shimura
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Satoh
authors.forename: Shin
authors.initials: S
authors.lastname: Kawano
authors.forename: Isao
authors.initials: I
authors.lastname: Tanaka
authors.forename: Masanobu
authors.initials: M
authors.lastname: Munekata
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002482:Cellulose / Q000096:biosynthesis*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D041822:Gluconacetobacter xylinus / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002482:Cellulose