title: Production of human erythropoietin by chimeric chickens.
abstract: The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white-hEpo was comparable to that produced by recombinant CHO cells.
authors.affiliation: Department of Biotechnology, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kodama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nishimiya
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Iwata
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yoshida
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.forename: Makoto
authors.initials: M
authors.lastname: Motono
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamashita
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Nishijima
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
authors.forename: Shinji
authors.initials: S
authors.lastname: Iijima
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D004527:Egg Proteins / Q000096:biosynthesis* / Q000235:genetics; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D004527:Egg Proteins; D011994:Recombinant Proteins; D004921:Erythropoietin