Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor.
To develop an integrated process of CO(2)-fixation and H(2) photoproduction by marine green microalga Platymonas subcordiformis, the impact of algal cells grown in CO(2)-supplemented air bubble column bioreactor was investigated on H(2) photoproduction regulated by carbonylcyanide m-chlorophenylhrazone. Highest cell growth (3.85 x 10(6) cells ml(-1)), starch content (0.25 +/- 0.08 mg per 10(6)cells) and hydrogen production (50 +/- 3 ml l(-1)) were achieved at 3% CO(2)-supplemented culture, which are respectively 1.4, 2.1, 1.5-fold of the air-supplemented culture. Improved H(2) production correlated well with the increase in starch accumulation. In this process, the algal cells have been recycled for stable H(2) production of 40-50 ml l(-1) over five cycles.
Guo
Zhen
Z
Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Chen
Zhaoan
Z
Zhang
Wei
W
Yu
Xingju
X
Jin
Meifang
M
D000693:Anaerobiosis; D019149:Bioreactors; D002245:Carbon Dioxide / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000378:metabolism*; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism*; D003624:Darkness; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D007700:Kinetics; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013997:Time Factors
D045332:Photosystem II Protein Complex; D002245:Carbon Dioxide; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D006859:Hydrogen; D013213:Starch; D006864:Hydrogenase