title: Engineering tissue tubes using novel multilayered scaffolds in the rat peritoneal cavity.
abstract: Our aim was to develop novel scaffolds to engineer tissue tubes of smooth muscle-like cells for autologous grafting. Small diameter tubular poly(lactic acid) scaffolds with randomly distributed, interconnected pores up to 100 mum were produced using a thermally induced phase separation method. The scaffolds were surface modified using various biomolecules via a layer-by-layer deposition technique, and implanted in the peritoneal cavities of rats. Histological analysis of scaffolds 3 weeks after implantation showed fully-developed tissue capsules on their outer surfaces, with macrophage-like cells present throughout the internal spaces. Surfaces coated in Matrigel supported the strongest cellular response whereas multilayer coatings with elastin, collagen I, collagen III, or chitosan outermost showed the lowest levels of cellular interaction. Although differences in capsule thickness and the presence or absence of cellularized layers on the inside and outside surfaces of the scaffolds were observed, none of these biomolecule coatings was able to overcome the foreign body response within the peritoneal cavity, even in the presence of a nonadsorptive HA undercoat.
authors.affiliation: Tissue Engineering and Microfluidics Laboratory, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Tristan
authors.initials: T
authors.lastname: Croll
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Rolfe
authors.forename: Julie H
authors.initials: JH
authors.lastname: Campbell
authors.forename: Gordon R
authors.initials: GR
authors.lastname: Campbell
authors.forename: Darren
authors.initials: D
authors.lastname: Martin
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Cooper-White
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D024061:Collagen Type III / Q000737:chemistry; D004338:Drug Combinations; D004549:Elastin / Q000737:chemistry; D020007:Implants, Experimental; D019344:Lactic Acid / Q000737:chemistry*; D007797:Laminin / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010529:Peritoneal Cavity; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D011509:Proteoglycans / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D024061:Collagen Type III; D004338:Drug Combinations; D007797:Laminin; D011091:Polyesters; D011108:Polymers; D011509:Proteoglycans; C059485:matrigel; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D004549:Elastin; D048271:Chitosan