Reciprocal subtraction differential RNA display (RSDD): an efficient technology for cloning differentially expressed genes.
Identification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological and pathological processes. Subtraction hybridization and differential RNA display (DDRT-PCR) are two methods that are widely and successfully employed to clone differentially expressed genes. Unfortunately, both methods have inherent problems and limitations requiring improvements in the technique. A combination of these two methods termed reciprocal subtraction differential RNA display is described here that considerably reduces the complexity of DDRT-PCR and facilitates the rapid and efficient identification and cloning of both abundant and rare differentially expressed genes.
Sarkar
Devanand
D
Department of Urology, Columbia University Medical Center, College of Physicians and Surgeons, New York, NY, USA.
Kang
Dong-chul
DC
Fisher
Paul B
PB
D015152:Blotting, Northern; D003001:Cloning, Molecular / Q000379:methods*; D004277:DNA, Single-Stranded; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D023281:Genomics / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012313:RNA
D004277:DNA, Single-Stranded; D012313:RNA