title: 8-isoprostane and leukotrienes in exhaled breath condensate in Czech subjects with silicosis.
abstract: Silicosis has progressive, but unpredictable development. There are no markers routinely available to measure the activity and prognosis of silicosis. 8-isoprostane, a marker of oxidative stress and leukotrienes B4, C4, D4, and E4 were measured in exhaled breath condensate in patients with silicosis. Sixty subjects were examined, with mean age 66.7+/-2.0 yr and mean exposure to silica 23.6+/-2.5 yr. The control group had 25 subjects, with mean age 64.7+/-4.8 yr. Exhaled breath was collected using the EcoScreen (Jaeger, Germany); 8-isoprostane and leukotrienes were analyzed by high-performance liquid chromatography/mass spectrometry. Several lung functions parameters were impaired in silicotics in comparisons with the controls; ESR, alpha1-antitrypsin and proteinuria were higher in the silicotics. Antineutrophilic cytoplasmic antibodies were more frequent in the silicotics (39%) than in the controls (4%), (p=0.0017). The mean level of 8-isoprostane in the patients with silicosis was 73.6+/-9.9 vs. 43+/-10 pg/ml (p=0.0001) in the controls. Silica exposure category (high, medium, none) was positively associated with the level of 8-isoprostane. In the patients with complicated silicosis, a high level of 8-isoprostane was found more frequently (p=0.0194). Leukotriene D4 was also increased in the silicotics (21.1+/-2.7 pg/ml) vs. the controls (14.7+/-2.7 pg/ml), (p=0.001). No significant effect of smoking or alcohol consumption on the markers examined was seen. This is the first study using exhaled breath condensate analysis in patients with silicosis.
authors.affiliation: Department of Occupational Medicine of the First Medical Faculty, Charles University, Prague, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Pelclová
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Fenclová
authors.forename: Petr
authors.initials: P
authors.lastname: Kacer
authors.forename: Tomá
authors.initials: T
authors.lastname: Navrátil
authors.forename: Marek
authors.initials: M
authors.lastname: Kuzma
authors.forename: Jindri Ka
authors.initials: JK
authors.lastname: Lebedová
authors.forename: Pavlína
authors.initials: P
authors.lastname: Klusácková
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests; D002851:Chromatography, High Pressure Liquid; D018153:Czech Republic; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D015438:Health Behavior; D006801:Humans; D015289:Leukotrienes / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D012129:Respiratory Function Tests; D012829:Silicosis / Q000175:diagnosis* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D015415:Biomarkers; D015289:Leukotrienes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost