title: Evolution of NMDA receptor cytoplasmic interaction domains: implications for organisation of synaptic signalling complexes.
abstract: BACKGROUND
  Glutamate gated postsynaptic receptors in the central nervous system (CNS) are essential for environmentally stimulated behaviours including learning and memory in both invertebrates and vertebrates. Though their genetics, biochemistry, physiology, and role in behaviour have been intensely studied in vitro and in vivo, their molecular evolution and structural aspects remain poorly understood. To understand how these receptors have evolved different physiological requirements we have investigated the molecular evolution of glutamate gated receptors and ion channels, in particular the N-methyl-D-aspartate (NMDA) receptor, which is essential for higher cognitive function. Studies of rodent NMDA receptors show that the C-terminal intracellular domain forms a signalling complex with enzymes and scaffold proteins, which is important for neuronal and behavioural plasticity
  
  
  RESULTS
  The vertebrate NMDA receptor was found to have subunits with C-terminal domains up to 500 amino acids longer than invertebrates. This extension was specific to the NR2 subunit and occurred before the duplication and subsequent divergence of NR2 in the vertebrate lineage. The shorter invertebrate C-terminus lacked vertebrate protein interaction motifs involved with forming a signaling complex although the terminal PDZ interaction domain was conserved. The vertebrate NR2 C-terminal domain was predicted to be intrinsically disordered but with a conserved secondary structure.
  
  
  CONCLUSIONS
  We highlight an evolutionary adaptation specific to vertebrate NMDA receptor NR2 subunits. Using in silico methods we find that evolution has shaped the NMDA receptor C-terminus into an unstructured but modular intracellular domain that parallels the expansion in complexity of an NMDA receptor signalling complex in the vertebrate lineage. We propose the NR2 C-terminus has evolved to be a natively unstructured yet flexible hub organising postsynaptic signalling. The evolution of the NR2 C-terminus and its associated signalling complex may contribute to species differences in behaviour and in particular cognitive function.
authors.affiliation: Genes to Cognition Program, Wellcome Trust Sanger Institute, Cambridge, UK. tr1@sanger.ac.uk
authors.forename: Tomás J
authors.initials: TJ
authors.lastname: Ryan
authors.forename: Richard D
authors.initials: RD
authors.lastname: Emes
authors.forename: Seth Gn
authors.initials: SG
authors.lastname: Grant
authors.forename: Noboru H
authors.initials: NH
authors.lastname: Komiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism*; D019143:Evolution, Molecular; D007448:Invertebrates / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D054731:PDZ Domains / Q000235:genetics; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016194:Receptors, N-Methyl-D-Aspartate / Q000737:chemistry* / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D009435:Synaptic Transmission / Q000235:genetics*
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; C121001:NR2B NMDA receptor