Lipophilic sugar nucleotide synthesis by structure-based design of nucleotidylyltransferase substrates.
Structure-based design of alkyl sugar-1-phosphates provides an efficient nucleotidylyltransferase-catalyzed synthesis of a series of new lipophilic sugar nucleotides possessing long or branched alkyl chains, thereby demonstrating the utility of nucleotidylyltransferases to catalyze the synthesis of sugar nucleotides with potential applications in lipopolysaccharide and lipoglycopeptide biosynthesis.
Huestis
Malcolm P
MP
College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, CanadaB3H 3J5.
Aish
Gaia A
GA
Hui
Joseph P M
JP
Soo
Evelyn C
EC
Jakeman
David L
DL
D001665:Binding Sites; D018360:Crystallography, X-Ray; D005947:Glucose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009711:Nucleotides / Q000138:chemical synthesis*; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D012210:Rhamnose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013403:Sugar Phosphates; D013942:Thymine Nucleotides / Q000737:chemistry / Q000378:metabolism
D009711:Nucleotides; D013403:Sugar Phosphates; D013942:Thymine Nucleotides; C103522:deoxythymidine diphosphate-glucose; D009713:Nucleotidyltransferases; D005947:Glucose; D012210:Rhamnose