Fluorescence-based detection of single nucleotide permutation in DNA via catalytically templated reaction.
Templated reduction of low fluorescence azidocoumarin-PNA conjugate to high fluorescence aminocoumarin was achieved using a catalytic amount of DNA with single nucleotide resolution.
Pianowski
Zbigniew L
ZL
Laboratoire de Chimie Organique et Bioorganique, Institut de Science et d'Ingénierie Supramoléculaires, Université Louis Pasteur-CNRS, 8 allée Gaspard Monge, 67083 Strasbourg, France.
Winssinger
Nicolas
N
D002384:Catalysis; D003374:Coumarins / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005453:Fluorescence; D008968:Molecular Conformation; D009154:Mutation; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013997:Time Factors
D003374:Coumarins; D020135:Peptide Nucleic Acids; D004247:DNA