[Quantification of circulating plasma DNA fragments as tumor markers in patients with esophageal and gastric cancer].
The quantity and quality of circulating DNA fragments was analyzed by quantitative real-time polymerase chain reactions (qPCR) in plasma from patients with esophageal and gastric cancer, in order to assess their diagnostic value. Plasma was collected preoperatively from 24 patients with esophageal cancer 53 patients with gastric cancer and from 21 healthy controls. qPCR was performed using two primer sets for the BETA-actin gene, amplifying short (102 bp) and long (253 bp) segments. The DNA concentrations in both the short and long segment assays of both cancer patients were significantly higher than the controls. The difference of concentrations between disease group and controls was more significant in esophageal cancer patients. The area under the receiver-operating characteristic curve was 0.83 (short) and 0.91 (long) for esophageal cancer patients, and 0.75 (short) and 0.67 (long) for gastric cancer versus the controls. There was also a significant difference in DNA integrity (short/long) between esophageal cancer patients and the control group (p = 0.001). qPCR assays for plasma DNA concentrations and their integrity can serve as new diagnostic markers for screening and monitoring patients with esophageal and gastric cancer.
Tomita
Haruhisa
H
Division of Digestive Surgery, Dept. of Surgery, Kyoto Prefectural University of Medicine.
Ichikawa
Daisuke
D
Sai
Soujin
S
Morimura
Rei
R
Murayama
Yasutoshi
Y
Komatsu
Shuhei
S
Ikoma
Daito
D
Tani
Nobuyuki
N
Ikoma
Hisashi
H
Fujiwara
Hitoshi
H
Kikuchi
Shojiro
S
Okamoto
Kazuma
K
Ochiai
Toshiya
T
Kokuba
Yukihito
Y
Sakakura
Chohei
C
Sonoyama
Teruhisa
T
Hagiwara
Akeo
A
Otsuji
Eigo
E
D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics*; D053938:DNA Fragmentation; D004938:Esophageal Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D006801:Humans; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology
D014408:Biomarkers, Tumor