Marchantin C, a macrocyclic bisbibenzyl, induces apoptosis of human glioma A172 cells.
Macrocyclic bisbibenzyls, a class of characteristic components derived from liverworts, are attracting more and more attention because of their wide range of biological significance, including anti-bacterial, anti-fungus, anti-oxidation and cytotoxicity. Herein, we investigated the pro-apoptotic effect of marchantin C on human glioma A 172 cells. The results demonstrated that marchantin C conferred dose-dependent inhibitory effects onto cell growth, viability and colony formation ability of A 172 cells. Morphological observation and DNA laddering assay showed that marchantin C-treated A172 cells displayed outstanding apoptosis characteristics, such as nuclear fragmentation, the appearance of membrane-enclosed apoptotic bodies and DNA laddering fragment. Moreover, flow cytometric detection of phosphatidylserine externalization indicated that marchantin C-induced apoptosis occurred in a dose-dependent manner. RT-PCR and western blot assay further substantiated that marchantin C, as a promising pro-apoptotic agent, had strong effects to induce A172 cell apoptosis, suggesting that the action was achieved through up-regulating Bax and down-regulating Bcl-2.
Shi
Yan-Qiu
YQ
Department of Natural Products Chemistry, School of Pharmaceutical Sciences, Shandong University, 44 Wen Hua Xi Road, Jinan, Shandong 250012, China.
Liao
Yong-Xiang
YX
Qu
Xian-Jun
XJ
Yuan
Hui-Qing
HQ
Li
Song
S
Qu
Jian-Bo
JB
Lou
Hong-Xiang
HX
D017209:Apoptosis / Q000187:drug effects*; D001632:Bibenzyls / Q000494:pharmacology* / Q000627:therapeutic use; D002396:Catechols / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004988:Ethers, Cyclic / Q000494:pharmacology* / Q000627:therapeutic use; D005910:Glioma / Q000188:drug therapy*; D006801:Humans; D010647:Phenyl Ethers / Q000494:pharmacology* / Q000627:therapeutic use; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D001632:Bibenzyls; D002396:Catechols; D004988:Ethers, Cyclic; D010647:Phenyl Ethers; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C459129:marchantin C