title: Invertebrate 7SK snRNAs.
abstract: 7SK RNA is a highly abundant noncoding RNA in mammalian cells whose function in transcriptional regulation has only recently been elucidated. Despite its highly conserved sequence throughout vertebrates, all attempts to discover 7SK RNA homologues in invertebrate species have failed so far. Here we report on a combined experimental and computational survey that succeeded in discovering 7SK RNAs in most of the major deuterostome clades and in two protostome phyla: mollusks and annelids. Despite major efforts, no candidates were found in any of the many available ecdysozoan genomes, however. The additional sequence data confirm the evolutionary conservation and hence functional importance of the previously described 3' and 5' stem-loop motifs, and provide evidence for a third, structurally well-conserved domain.
authors.affiliation: Institute for Theoretical Chemistry, University of Vienna, Währingerstrasse 17, A-1090 Wien, Austria. agruber@tbi.univie.ac.at
authors.forename: Andreas R
authors.initials: AR
authors.lastname: Gruber
authors.forename: Dorota
authors.initials: D
authors.lastname: Koper-Emde
authors.forename: Manja
authors.initials: M
authors.lastname: Marz
authors.forename: Hakim
authors.initials: H
authors.lastname: Tafer
authors.forename: Stephan
authors.initials: S
authors.lastname: Bernhart
authors.forename: Gregor
authors.initials: G
authors.lastname: Obernosterer
authors.forename: Axel
authors.initials: A
authors.lastname: Mosig
authors.forename: Ivo L
authors.initials: IL
authors.lastname: Hofacker
authors.forename: Peter F
authors.initials: PF
authors.lastname: Stadler
authors.forename: Bernd-Joachim
authors.initials: BJ
authors.lastname: Benecke
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D006801:Humans; D007448:Invertebrates / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D011401:Promoter Regions, Genetic / Q000235:genetics; D016372:RNA, Antisense / Q000235:genetics; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014714:Vertebrates / Q000235:genetics
chemical_list: D016372:RNA, Antisense; D012342:RNA, Small Nuclear