Expression of natriuretic peptide-activated guanylate cyclases by cholinergic and dopaminergic amacrine cells of the rat retina.
Recently, the natriuretic peptides were detected in the cholinergic and dopaminergic amacrine cells of the retina. We performed immunofluorescence labeling of rat retinal sections to examine the immunoreactivity of natriuretic peptide-activated guanylate cyclases (NPR-A and NPR-B) in the rat retina, in particular whether they were localized to dopaminergic and cholinergic amacrine cells. NPR-A and NPR-B immunoreactivity was detected in several layers of the retina including amacrine cells. In amacrine cells, both NPR-A and NPR-B were co-localized with tyrosine hydroxylase, a marker of dopaminergic cells. NPR-B, but not NPR-A, was localized to amacrine cells expressing choline acetyltransferase (ChAT), a marker of cholinergic cells. These findings suggest that natriuretic peptides have different regulatory systems in dopaminergic and cholinergic amacrine cells in rat retina.
Abdelalim
Essam Mohamed
EM
Molecular Neuroscience Research Center, Shiga University of Medical Science, Seta, Otsu, Shiga 520-2192, Japan.
Masuda
Chiaki
C
Tooyama
Ikuo
I
D000109:Acetylcholine / Q000378:metabolism*; D025042:Amacrine Cells / Q000201:enzymology*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D008297:Male; D045265:Natriuretic Peptides / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000032:analysis / Q000378:metabolism*
D045265:Natriuretic Peptides; D002795:Choline O-Acetyltransferase; D017461:Receptors, Atrial Natriuretic Factor; D000109:Acetylcholine; D004298:Dopamine