title: Printing protein arrays from DNA arrays.
abstract: We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
authors.affiliation: Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK.
authors.forename: Mingyue
authors.initials: M
authors.lastname: He
authors.forename: Oda
authors.initials: O
authors.lastname: Stoevesandt
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Palmer
authors.forename: Farid
authors.initials: F
authors.lastname: Khan
authors.forename: Olle
authors.initials: O
authors.lastname: Ericsson
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Taussig
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D020869:Gene Expression Profiling / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*