CpG motif-based adjuvant as a replacement for Freund's complete adjuvant in a recombinant LHRH vaccine.
This study compared: (1) Freund's complete adjuvant and CpG oligodeoxynucleotide (ODN) 2006 in water-in-oil emulsion as adjuvants; and (2) increasing doses of a recombinant ovalbumin-LHRH (ova-LHRH) fusion protein as an antigen for a contraceptive vaccine. Treatment groups (n=8 heifers/group) were: one untreated control group; five groups receiving CpG ODN with different doses of ova-LHRH (1.5; 2.3; 3.4; 5.1; and 7.6 mg); and one group receiving 3.4 mg ova-LHRH in Freund's. Heifers were immunized at weeks 0 and 14. All vaccine treatments caused gonadal regression and estrus suppression. CpG ODN is a suitable replacement for Freund's for LHRH immunization.
Conforti
Valéria A
VA
Department of Animal Sciences, Washington State University, Pullman, WA 99164-6310, USA. valeria.conforti@cincinnatizoo.org
de Avila
David M
DM
Cummings
Nada S
NS
Zanella
Ricardo
R
Wells
Kenny J
KJ
Ulker
Hasan
H
Reeves
Jerry J
JJ
D000276:Adjuvants, Immunologic; D000818:Animals; D002417:Cattle; D018899:CpG Islands; D005260:Female; D005620:Freund's Adjuvant; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D009838:Oligodeoxyribonucleotides; D010047:Ovalbumin / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013245:Sterilization, Reproductive / Q000662:veterinary*; D022642:Vaccines, Contraceptive / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D022642:Vaccines, Contraceptive; D014614:Vaccines, Synthetic; D007987:Gonadotropin-Releasing Hormone; D010047:Ovalbumin; D005620:Freund's Adjuvant