title: Influence of cancer-related gene polymorphisms on clinicopathological features in colorectal cancer.
abstract: OBJECTIVE
  Single nucleotide polymorphisms (SNP) are shown to be related with cancer incidence. It has been reported that CCND1, p21(cip1)DCC, MTHFR, and EXO1 are related with the risk of malignant neoplasm, but few studies have mentioned the prognosis of the patients. We investigated the SNP of patients and related this to clinicopathological features, including survival rate.
  
  
  METHODS
  DNA from the tissues of primary colorectal cancer was obtained from surgical resections of 114 patients (68 males and 46 females, 29-83 years). The CCND1 polymorphism was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and those of other genes were investigated by the TaqMan method. The polymorphisms obtained were statistically analyzed for the relationship with clinicopathological features.
  
  
  RESULTS
  The CG + GG allele was more invasive than the CC allele in histological tumor depth in the DCC codon 201 (P = 0.0086). The 677TT allele in MTHFR had a larger tumor size than the 677CC allele (P = 0.028). In EXO1 P757L polymorphism, patients with the TT allele had a statistically reduced survival rate compared with the other alleles. In CCND1 polymorphisms, we found no statistical significance in clinicopathological features.
  
  
  CONCLUSIONS
  From these preliminary data, these polymorphisms would be candidates predicting the clinicopathological features of colorectal cancer, but further more systematic gene analyses are warranted.
authors.affiliation: Department of Gastroenterology and Hematology, Hirosaki University Graduate School of Medicine, Aomori, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Yoshiya
authors.forename: Takenori
authors.initials: T
authors.lastname: Takahata
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Hanada
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishiguro
authors.forename: Masato
authors.initials: M
authors.lastname: Saito
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Sasaki
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D056741:Cyclin D; D016213:Cyclins / Q000235:genetics; D005260:Female; D016163:Genes, DCC; D005838:Genotype; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D056741:Cyclin D; D016213:Cyclins; D042965:Methylenetetrahydrofolate Reductase (NADPH2)