Optical flow-through sensor for the determination of norfloxacin based on emission of KMnO(4)-Na (2)SO (3)-Tb (3+) system.
A simple and selective method to determine norfloxacin using an optical flow-through sensor has been developed. The present sensor was prepared by packing anionic ion exchange resin in a glass tube, followed by introducing KMnO(4) solution to the glass tube for immobilization on resin. The optical sensor is based on the emission intensity from the Tb(III) solution sensitized by norfloxacin. The excitation of norfloxacin occurred by the chemiluminescence from the reaction of KMnO(4) and Na(2)SO(4) solutions. The effects of pH, concentration of Tb(III) ion, KMnO(4) and Na(2)SO(4) solutions and flow rate of the norfloxacin solution on the chemiluminescence intensity were studied to find the optimum experimental conditions. The emission intensity increased linearly with increasing norfloxacin concentration from 1.0 x 10(-3) to 1.0 x 10(-8) M and the detection limit (3sigma) was 8.7 x 10(-9). The applicability of the present method was demonstrated by determination of norfloxacin in various pharmaceutical preparations and serum sample.
Jeon
Chi Wan
CW
Korea Institute of Geosciences and Mineral Resources, Daejeon, 305-350, South Korea.
Khan
Mir Azam
MA
Lee
Sang Hak
SH
Karim
Mohammad Mainul
MM
Lee
Hee Kyung
HK
Suh
Yeoun Suk
YS
Alam
Seikh Mafiz
SM
Chung
Hye Young
HY
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods; D015394:Molecular Structure; D009643:Norfloxacin / Q000032:analysis* / Q000737:chemistry; D011196:Potassium Permanganate / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013447:Sulfites / Q000032:analysis*; D013725:Terbium / Q000032:analysis*
D000900:Anti-Bacterial Agents; D013447:Sulfites; D011196:Potassium Permanganate; D013725:Terbium; D009643:Norfloxacin; C025026:sodium sulfite