Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum.
Polysaccharides were one of the main components of Ganoderma lucidum, a medicinal mushroom well known for its immuno-modulation effects. In the present study, we demonstrated that polysaccharides extracted from Ganoderma lucidum spores (GL-SP) could stimulate splenic mononuclear cells (MNCs) proliferation and cytokine production. To identify the possible cellular targets of GL-SP in MNCs, two-dimensional gel electrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after GL-SP treatment were identified by MALDI-TOF MS/MS. Ten proteins with >2-fold increase or decrease expression in GL-SP-treated MNCs compared with control were found and further identified by MALDI-TOF MS/MS analysis and database searching. In the GL-SP-treated MNCs, there were increases in the expression of myosin regulatory light chain 2-A, Rho GDP dissociation inhibitor beta, T-cell-specific GTPase, phosphatidylinositol transfer protein alpha, and decreases in the expression of apoptosis-associated speck-like protein containing a CARD, 14-3-3 tau, beta-actin, tubulin alpha 2, copine I, and gamma-actin. The results of the present study help to provide insight into the immuno-modulating activities of GL-SP.
Ma
Chao
C
Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, PR China.
Guan
Shu-Hong
SH
Yang
Min
M
Liu
Xuan
X
Guo
De-An
DA
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D040901:Proteomics; D020940:Reishi / Q000737:chemistry*; D013154:Spleen / Q000276:immunology; D013172:Spores, Fungal / Q000737:chemistry*; D048069:Tumor Necrosis Factors / Q000378:metabolism
D007376:Interleukin-2; D011134:Polysaccharides; D048069:Tumor Necrosis Factors