Anti-genotoxic effect of hydrated sodium calcium aluminosilicate on genotoxicity to human lymphocytes induced by aflatoxin B1.
The anti-genotoxic potential of hydrated sodium calcium aluminosilicate (HSCAS) was investigated using sister chromatid exchanges (SCEs) induced by aflatoxin B1 (AFB1) as genotoxic endpoint in human lymphocytes. Whole blood samples from two healthy male donors were used for this experiment and the effects of different concentrations of HSCAS (5 x 10(-6) and 1 x 10(-5) M) and AFB1 (1, 5 and 10 microM) were tested. The present results established that the frequencies of SCEs (at doses of 5 and 10 microM except for 1 microM) in lymphocytes were significantly increased by AFB1 compared to controls. When HSCAS was added alone at a molar ratio of 5 x 10(-6) and 1 x 10(-5), lymphocytes did not show significant increases in SCE frequencies, but SCE frequencies induced by the various concentrations of AFB1 could be significantly reduced by the presence of HSCAS (at both doses). In fact, HSCAS at 1 x 10(-5) M completely inhibited SCE formations caused by AFB1. These results suggest for the first time, that HSCAS can antagonize the ability of AFB1 to cause DNA damage that leads to the formation of SCEs.
Türkez
Hasan
H
Department of Biology, Science and Art Faculty, Atatürk University, 25240 Erzurum, Turkey.
Sişman
Turgay
T
D000328:Adult; D016604:Aflatoxin B1 / Q000737:chemistry / Q000633:toxicity*; D000538:Aluminum Silicates / Q000008:administration & dosage / Q000737:chemistry; D000704:Analysis of Variance; D000992:Antitoxins / Q000008:administration & dosage* / Q000737:chemistry; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D018709:Statistics, Nonparametric; D017641:Zeolites / Q000008:administration & dosage* / Q000737:chemistry
D000538:Aluminum Silicates; D000992:Antitoxins; D009153:Mutagens; D017641:Zeolites; C087965:sodium calcium aluminosilicate, hydrated; D016604:Aflatoxin B1