Combination of two hydrophilic interaction chromatography methods that facilitates identification of 2-aminobenzamide-labeled oligosaccharides.
Hydrophilic interaction chromatography (HILIC) is used to separate 2-aminobenzamide- (2-AB) labeled N-linked oligosaccharides. The glycans of the model protein, bovine fetuin, are identified following comparison of elution patterns of seven 2-AB-labeled glycan standards, of which two are of the high-mannose type and five are of the complex type. The combination of two HILIC methods, using an Amide-80 column, having different resolutions and selectivities, markedly facilitates the identification of the fetuin glycans. These HILIC methods are suitable for obtaining glycan profiles of complex mixtures.
Karlsson
Göran
G
Octapharma AB, SE-11275 Stockholm, Sweden. goran.karlsson@octapharma.se
Swerup
Elin
E
Sandberg
Helena
H
D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D000509:alpha-Fetoproteins / Q000032:analysis / Q000737:chemistry
D009844:Oligosaccharides; D011134:Polysaccharides; D000509:alpha-Fetoproteins