[Immunohistochemical analysis of the lens cells during development of the various types of age-associated cataract in man].
The objective of this investigation was a comparative immunohistochemical study of the crystalline eye lens under normal conditions and in different types of age-associated cataract in man. Control group included the lenses of young men, who died in the accidents and served as lens donors (n = 10, material collected within 1 hr since death). The lens was taken from the patients (age: 60-70 years) during the operation on the occasion of cortical (n = 20) and nuclear (n = 20) cataract. The study has demonstrated phenotype plasticity of lens cells in different types of age-associated cataract. The changes of cell phenotype were shown to depend on cataract type. During the development of cortical age-associated cataract, positive reaction with the monoclonal antibodies against neuron-specific enolase, S-100 protein and vimentin was detected only within the lens cortex. During the formation of nuclear age-associated cataract, positive reaction with the antibodies against alpha-smooth muscle actin, pancytokeratin was found only within the lens nucleus. These data suggest that the differentiated approach to the treatment of different types of age-associated cataract should be worked out.
Korsakova
N V
NV
Sergeeva
V E
VE
Petrov
S B
SB
D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology*; D002386:Cataract / Q000378:metabolism / Q000473:pathology*; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D009418:S100 Proteins; D014746:Vimentin; D010751:Phosphopyruvate Hydratase