Growth enhancement of shrimp (Litopenaeus schmitti) after transfer of tilapia growth hormone gene.
Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyses. Western blot analyses revealed that 34% of the screened larvae expressed a single tiGH-specific band with the expected molecular mass (23.1 kDa). The development index and larval length indicated a significant growth enhancement from day 3 on after electroporation, with an average of 32% of the growth enhancement. To our knowledge, this is the first report on gene transfer enhanced growth in crustaceans.
Arenal
Amilcar
A
Center of Genetic Engineering and Biotechnology, P.O. Box 387, ZIP 70100, Camaguey 1, Cuba. amilcar.arenal@cigb.edu.cu
Pimentel
Rafael
R
Pimentel
Eulogio
E
Martín
Leonardo
L
Santiesteban
Dayamí
D
Franco
Ramón
R
Aleström
Peter
P
D000818:Animals; D030801:Animals, Genetically Modified; D018274:Electroporation; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D024861:Genetic Enhancement; D013006:Growth Hormone / Q000235:genetics*; D019913:Nuclear Localization Signals / Q000235:genetics; D033561:Penaeidae / Q000235:genetics* / Q000254:growth & development*; D010957:Plasmids; D017210:Tilapia / Q000235:genetics*; D013997:Time Factors; D019076:Transgenes
D019913:Nuclear Localization Signals; D013006:Growth Hormone