Identification of biological microparticles using ultrafast depletion spectroscopy.
We show how an ultrafast pump-pump excitation induces strong fluorescence depletion in biological samples, such as bacteria-containing droplets, in contrast with fluorescent interferents, such as polycyclic aromatic compounds, despite similar spectroscopic properties. Application to the optical remote discrimination of biotic versus non-biotic particles is proposed. Further improvement is required to allow the discrimination of one pathogenic among other non-pathogenic micro-organisms. This improved selectivity may be reached with optimal coherent control experiments, as discussed in the paper.
Courvoisier
Francois
F
GAP-Biophotonics, University of Geneva, 20, rue de l'Ecole de Médecine, 1211, Geneva 4, Switzerland.
Bonacina
Luigi
L
Boutou
Véronique
V
Guyon
Laurent
L
Bonnet
Christophe
C
Thuillier
Benoit
B
Extermann
Jerome
J
Roth
Matthias
M
Rabitz
Herschel
H
Wolf
Jean-Pierre
JP
D000336:Aerosols / Q000737:chemistry; D001419:Bacteria / Q000302:isolation & purification; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000336:Aerosols