title: Microbial community that catalyzes partial nitrification at low oxygen atmosphere as revealed by 16S rRNA and amoA genes.
abstract: We previously reported that partial nitrification in the down-flow hanging sponge (DHS) system was satisfactorily accomplished under oxygen-limited conditions [Chuang et al., Water Res., 41, 295-302 (2007)]. In this study, we investigated the microbes that are responsible for the partial nitrification in this unique system by 16S rRNA- and amoA-based cloning analyses and fluorescence in situ hybridization. Microbes related to Nitrosomonas species were found to be chiefly responsible for catalyzing the partial nitrification. Microbes affiliated with the uncultivated phyla OP10 and Bacteroidetes were also numerous in the DHS, but their ecological niches are still unknown.
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, 1603-1 Kamitomiokamachi, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Chuang
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imachi
authors.forename: Madan
authors.initials: M
authors.lastname: Tandukar
authors.forename: Shuji
authors.initials: S
authors.lastname: Kawakami
authors.forename: Hideki
authors.initials: H
authors.lastname: Harada
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Ohashi
mesh_terms: D041963:Bacteroidetes / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D009573:Nitrites / Q000378:metabolism*; D009606:Nitrosomonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D010100:Oxygen