Comparative structural analysis of the binding domain of follicle stimulating hormone receptor.
Proteins with leucine-rich repeats (LRRs) specialize in mediating protein-protein interactions. The hormone binding portion of the receptor for follicle stimulating hormone (FSH) is an LRR protein by sequence, and the crystal structure of this domain from human FSH receptor in a complex with FSH shows that it does indeed have an LRR structure. It differs from other LRR domains, however, in being an all-beta protein composed of highly irregular repeats and having only slight overall curvature. Despite these distinctions and a superficial resemblance to beta-helical proteins, the binding domain of FSH receptor clearly is an LRR protein. The structure does consist of two parts with distinctively different curvatures. Comparison with the structures of other LRR-containing proteins shows a correlation between curvature and main-chain hydrogen bonding pattern of the parallel beta-sheet. The hormone-binding site is located at the concave surface of the receptor structure, a feature common to proteins with LRR motifs. Analysis of the ligand-binding site of LRR-containing proteins reveals that they generally utilize extensive interface area and a large number of charged residues to facilitate high-affinity protein-protein interactions.
Fan
Qing R
QR
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Hendrickson
Wayne A
WA
D000595:Amino Acid Sequence; D006801:Humans; D006860:Hydrogen Bonding; D000090263:Leucine-Rich Repeat Proteins; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011962:Receptors, FSH / Q000737:chemistry*; D020449:Repetitive Sequences, Amino Acid; D017386:Sequence Homology, Amino Acid
D000090263:Leucine-Rich Repeat Proteins; D008024:Ligands; D011506:Proteins; D011962:Receptors, FSH