[An experimental study of a rat model with MODS as a result of trauma induced infection].
OBJECTIVE
To reproduce an animal model of multiple organ dysfunction syndrome (MODS) which was caused by two "hits" (injury and infection), to explore the potential aetiology and strategies of treatment.


METHODS
The rats' extremities were crushed, resulting in severe injury with multiple closed fractures and extensive contusion of soft tissues. After 12 hours, a thirty percent total body surface area III (TBSA III) burns contaminated by Pseudomonas aeruginosa was inflicted upon the injury rats in MODS group. Rats'activities and wound appearance were observed, body weight, temperature, heart rate were recorded, and the blood lipopolysaccharide (LPS) level, the functional changes in all major organs, the occurrence rate of inflammatory reactions, the mortality of MODS and morbidity, and the pathological changes of the major organs were monitored at 24, 48, 96, 120 hours.


RESULTS
To compare with pre-injury states and control group there were marked increases in alanine aminotransferase (ALT), total bilirubin (TBil), blood urea nitrogen (BUN), creatinine (Cr), aspartate aminotransferase (AST), creatine phosphokinase (CPK) contents 48 hours after the injuries (all P<0.05). When compared with the control group, there was significant difference (P<0.05). After 48 hours, there were obvious changes in pathology, the rats were in the early stage of MODS along with signs of damage to several organs. There was a typical relationship between LPS and organ functional changes. The LPS level peaked within 96 hours after the injury, the level was 8.36 folds of basic level. After 96 hours, there was correlation between LPS and the change of organ function (r=0.927 2). The incidence of MODS was 86%, and the mortality rate reached 30% in 96 hours after injury. At 120 hours after the injury, MODS was found in all the rats and the mortality rate reached 50%.


CONCLUSIONS
This model seems to mimick the development of MODS which occurs after serious injuries followed by infection. The process of infection is coincidental with clinical picture, and LPS was released steadily with full body reaction. This animal model provides us an excellent opportunity to explore the pathogenesis and treatment of MODS after trauma.
Ban
Yu
Y
Department of Emergency, General Hospital of The PLA, Beijing 100853, China.
Shen
Hong
H
Li
Tan-shi
TS
D000818:Animals; D004195:Disease Models, Animal; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014946:Wound Infection / Q000150:complications*; D014947:Wounds and Injuries / Q000150:complications