Molecular characterization of astrovirus in stool samples from children in São Paulo, Brazil.
The purpose of this study was to characterize astrovirus in faecal samples collected from children with and without diarrhea in São Paulo, Brazil, grouped into two sets: EPM and HU. Detection and genotyping were carried out using reverse transcription nested polymerase chain reaction (RT-PCR) with specific primers directed towards the genome open reading frame 2 (ORF2). Results for EPM set showed that 66/234 (28.2%) were positive: 28/94 (29.7%) from children with acute diarrhea, 14/45 (31.1%) with persistent diarrhea, and 9/55 (16.3%) from control individuals. No data was available for 15/40 (37.5%) of samples. Mixed infections with other viruses were found in 33 samples. In the HU, 18/187 (9.6%) were positive: 12/158 (7.6%) from individuals with acute diarrhea and 6/29 (20.7%) from control children. Four samples were mixed with other viruses. Out of 66 astrovirus positive EPM samples, 18 (27.2%) were characterized as human astrovirus type-1 (HAstV-1), two (3.0%) as HAstV-2, two (3.0%) as HAstV-3, and three (4.5%) as HAstV-8. Among 18 astrovirus positive HU samples, one (5.5%) was characterized as HAstV-1, six (33.3%) as HAstV-2, and one (5.5%) as HAstV-8. Two HAstV-8 genotyped samples were further confirmed by nucleotide sequencing. Our results shows that astroviruses are circulating in a constant manner in the population, with multiple serotypes, in higher frequency than it was described for other Brazilian regions. For the first time in Sao Paulo, Brazil, it was shown that astroviruses play an important role in children gastroenteritis, as described for most locations where they were detected.
Resque
Hugo Reis
HR
Laboratório de Gastroenterites Virais, Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brasil.
Munford
Veridiana
V
Castilho
Juliana Galera
JG
Schmich
Herika
H
Caruzo
Thabata Alessandra Ramos
TA
Rácz
Maria Lucia
ML
D000208:Acute Disease; D019350:Astroviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D001938:Brazil / Q000453:epidemiology; D016022:Case-Control Studies; D002648:Child; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D001257:Mamastrovirus / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral