Towards defining the nuclear proteome.
BACKGROUND
The nucleus is a complex cellular organelle and accurately defining its protein content is essential before any systematic characterization can be considered.


RESULTS
We report direct evidence for 2,568 mammalian proteins within the nuclear proteome: the nuclear subcellular localization of 1,529 proteins based on a high-throughput subcellular localization protocol of full-length proteins and an additional 1,039 proteins for which clear experimental evidence is documented in published literature. This is direct evidence that the nuclear proteome consists of at least 14% of the entire proteome. This dataset was used to evaluate computational approaches designed to identify additional nuclear proteins.


CONCLUSIONS
This represents direct experimental evidence that the nuclear proteome consists of at least 14% of the entire proteome. This high-quality nuclear proteome dataset was used to evaluate computational approaches designed to identify additional nuclear proteins. Based on this analysis, researchers can determine the stringency and types of lines of evidence they consider to infer the size and complement of the nuclear proteome.
Fink
J Lynn
JL
ARC Centre of Excellence in Bioinformatics, The University of Queensland, St Lucia, Queensland, 4072, Australia.
Karunaratne
Seetha
S
Mittal
Amit
A
Gardiner
Donald M
DM
Hamilton
Nicholas
N
Mahony
Donna
D
Kai
Chikatoshi
C
Suzuki
Harukazu
H
Hayashizaki
Yosihide
Y
Teasdale
Rohan D
RD
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D019295:Computational Biology / Q000379:methods; D006801:Humans; D009687:Nuclear Proteins; D020543:Proteome
D009687:Nuclear Proteins; D020543:Proteome