Use of nanopatterned surfaces to enhance immunoreaction efficiency.
In this work, we compare the immunoreaction efficiency between uniformly functionalized surface and chemically nanopatterned surfaces when applied as platforms for antigen/antibody interactions with and without the use of protein A as orienting protein. On the nanopatterned platform, the immunoreaction efficiency is higher than all the other cases with no protein A pretreatment of the surface, providing evidence of the capability of the adhesive/antiadhesive nanopatterned surface to immobilize the molecules in a reactive state, increasing their possibility to form complexes.
Valsesia
A
A
European Commission Joint Research Centre, Institute for Health and Consumer Protection, 21020 Ispra (Varese), Italy.
Colpo
P
P
Mannelli
I
I
Mornet
S
S
Bretagnol
F
F
Ceccone
G
G
Rossi
F
F
D000941:Antigens / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry; D013499:Surface Properties
D000941:Antigens; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A