title: Comparison of results obtained with Amplicor HIV-1 DNA PCR test version 1.5 using 100 versus 500 microliters of whole blood.
abstract: The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 microl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 microl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.
authors.affiliation: Department of Pathology, 600 North Wolfe St., Johns Hopkins Medical Institutions, Baltimore, MD 21287, USA.
authors.forename: Estelle
authors.initials: E
authors.lastname: Piwowar-Manning
authors.forename: Lebah
authors.initials: L
authors.lastname: Lugalia
authors.forename: Bosco
authors.initials: B
authors.lastname: Kafufu
authors.forename: J Brooks
authors.initials: JB
authors.lastname: Jackson
mesh_terms: D001769:Blood / Q000821:virology; D001800:Blood Specimen Collection / Q000379:methods*; D004279:DNA, Viral / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral