title: Determination of terephthalic acid isopropylamide in urine with a liquid chromatography/mass spectrometry (LC/MS) method.
abstract: A sensitive and simple liquid chromatography/mass spectrometry (LC/MS) method was developed for the determination of terephthalic acid isopropylamide, the final metabolite of procarbazine in human urine. A solid-phase extraction with C(18) cartridges was used followed by LC/MS with a single mass spectrometer (SSQ 7000 from Finnigan). Terephthalic acid isobutylamide was the internal standard. The quantification limit was 30 ng/mL in urine (6 x noise). This assay was applied for drug monitoring of terephthalic acid isopropylamide in urine after oral administration of procarbazine in children and adolescents with Hodgkin lymphomas.
authors.affiliation: Institute of Clinical Pharmacology, University of Leipzig, Leipzig, Germany. F.Baumann@medizin.uni-leipzig.de
authors.forename: F
authors.initials: F
authors.lastname: Baumann
authors.forename: C
authors.initials: C
authors.lastname: Mauz-Körholz
authors.forename: D
authors.initials: D
authors.lastname: Clauss
authors.forename: S
authors.initials: S
authors.lastname: Borrmann
authors.forename: A
authors.initials: A
authors.lastname: Giannis
authors.forename: N
authors.initials: N
authors.lastname: Merkel
authors.forename: D
authors.initials: D
authors.lastname: Körholz
authors.forename: R
authors.initials: R
authors.lastname: Preiss
mesh_terms: D000293:Adolescent; D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring; D004355:Drug Stability; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010795:Phthalic Acids / Q000737:chemistry / Q000652:urine*; D011344:Procarbazine / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology
chemical_list: D010795:Phthalic Acids; D011344:Procarbazine; C011363:terephthalic acid; C528395:terephthalic acid isopropylamide