title: Microfluidic devices for studying growth and detachment of Staphylococcus epidermidis biofilms.
abstract: Microfluidic devices were used to study the influences of hydrodynamics of local microenvironments on Staphylococcus epidermidis (S. epidermidis) biofilm formation and the effects of a poly(beta-1,6-N-acetyl glucosamine)-hydrolyzing enzyme (dispersin B) and/or an antibiotic (rifampicin) on the detachment of the biofilm. Elongated, monolayered biofilm morphologies were observed at high flow velocity and fluid shear locations whereas large clump-like, multilayered biofilm structures were produced at low flow velocity and fluid shear locations. Upon dispersin B treatment, most of the biofilm was detached from the microchannel surface. However, a trace amount of bacterial cells could not be removed from corner locations most likely due to the insufficient wall shear stress of the fluid at these locations. Dispersin B or rifampicin treatment was effective in delaying the dispersal behavior of bacterial cells, but could not completely remove the biofilm. Combined dynamic delivery of dispersin B and rifampicin was found to be effective for complete removal of the S. epidermidis biofilm.
authors.affiliation: New Jersey Center for MicroChemical Systems, Department of Chemical, Biomedical and Materials Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
authors.forename: Joung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Kaplan
authors.forename: Woo Y
authors.initials: WY
authors.lastname: Lee
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002021:Buffers; D002364:Caseins / Q000737:chemistry; D003198:Computer Simulation; D004347:Drug Interactions; D004867:Equipment Design / Q000295:instrumentation; D006026:Glycoside Hydrolases / Q000494:pharmacology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010710:Phosphates / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry; D012293:Rifampin / Q000494:pharmacology; D012965:Sodium Chloride / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D002364:Caseins; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D011492:Protein Hydrolysates; C113831:poly-N-acetyl glucosamine; C026375:trypticase-soy broth; D012965:Sodium Chloride; D006026:Glycoside Hydrolases; D000117:Acetylglucosamine; D012293:Rifampin