cDNA cloning and expression of a xenobiotic metabolizing cytochrome P4501A (CYP1A) from the yellow catfish, Pelteobagrus fulvidraco (Siluriformes).
We cloned the full-length cDNA sequence of xenobiotic metabolizing cytochrome P4501A (CYP1A) gene from the yellow catfish (Pelteobagrus fulvidraco, Siluriformes, Bagridae). P. fulvidraco is a commercially important fish generally distributed in Southeast Asian countries and Korea. This is the first report of any xenobiotic metabolizing gene from this fish species. Tissue distribution of CYP1A was studied by real-time PCR. Induction pattern of CYP1A was studied by exposing fish to beta-naphthoflavone (BNF). Liver showed the highest level of expression in control as well as BNF-treated fish. However, high levels of expression were also recorded in brain, intestine, and kidney and the least in the muscles. Information on CYP genes in fish from order Siluriformes is limited as compared with other fish orders. This study provides an insight into the xenobiotic metabolizing system of P. fulvidraco and offers baseline information for further research related to biomarker use of CYP1A and detoxification strategies of this commercially important fish species.
Kim
Jin-Hyoung
JH
Department of Marine Science, College of Natural Sciences, Inha University, Incheon 402-751, South Korea.
Hwang
Dae-Sik
DS
Son
Kyu-Hee
KH
Raisuddin
Sheikh
S
Ki
Jang-Seu
JS
Lee
Jae-Seong
JS
Han
Kyung-Nam
KN
D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics*; D003001:Cloning, Molecular; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D019324:beta-Naphthoflavone / Q000494:pharmacology
D018076:DNA, Complementary; D012333:RNA, Messenger; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1