Potential for clinical ex vivo expansion of cord blood haemopoietic stem cells using non-haemopoietic factor supplements.
The establishment of culture systems that promote haemopoietic stem cell (HSC) self-renewal and expansion ex vivo will increase the clinical potential of umbilical cord blood (CB) HSC transplantation. Studies defining key signalling pathways that regulate development and expansion of HSC in vivo have greatly facilitated development of protocols for expanding HSC in ex vivo culture. Recently a number of soluble factors with novel stem cell expansion activity have been identified as part of pathways associated with mesodermal induction, or as factors produced by supportive stroma. These have been reported to support, to varying degrees, HSC self-renewal under in vitro conditions. Here we review the activities of these new factors and consider their future potential as components in ex vivo expansion culture for CB HSC. Finally we discuss the challenges associated with applying these factors to clinically relevant culture systems.
Hutton
Jonathon F
JF
Division of Haematology and Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, 5000, South Australia.
D'Andrea
Richard J
RJ
Lewis
Ian D
ID
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D018929:Cell Culture Techniques; D018920:Coculture Techniques; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology*; D016298:Hematopoietic Cell Growth Factors / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction; D014212:Tretinoin / Q000378:metabolism; D051153:Wnt Proteins / Q000378:metabolism
D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors; D036341:Intercellular Signaling Peptides and Proteins; D051880:Receptors, Notch; D051153:Wnt Proteins; D014212:Tretinoin