title: Gold nanostoves for microsecond DNA melting analysis.
abstract: In traditional DNA melting assays, the temperature of the DNA-containing solution is slowly ramped up. In contrast, we use 300 ns laser pulses to rapidly heat DNA bound gold nanoparticle aggregates. We show that double-stranded DNA melts on a microsecond time scale that leads to a disintegration of the gold nanoparticle aggregates on a millisecond time scale. A perfectly matching and a point-mutated DNA sequence can be clearly distinguished in less than one millisecond even in a 1:1 mixture of both targets.
authors.affiliation: Photonics and Optoelectronics Group, Physics Department, CeNS, Ludwig-Maximilians-Universität München, Amalienstrasse 54, 80799 Munich, Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Stehr
authors.forename: Calin
authors.initials: C
authors.lastname: Hrelescu
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Sperling
authors.forename: Gunnar
authors.initials: G
authors.lastname: Raschke
authors.forename: Michael
authors.initials: M
authors.lastname: Wunderlich
authors.forename: Alfons
authors.initials: A
authors.lastname: Nichtl
authors.forename: Dieter
authors.initials: D
authors.lastname: Heindl
authors.forename: Konrad
authors.initials: K
authors.lastname: Kürzinger
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Klar
authors.forename: Jochen
authors.initials: J
authors.lastname: Feldmann
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D006046:Gold / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D017422:Sequence Analysis, DNA / Q000379:methods*; D013499:Surface Properties; D044366:Transition Temperature
chemical_list: D046911:Macromolecular Substances; D006046:Gold; D004247:DNA