title: The -759C/T polymorphism of the 5-HT2C receptor is associated with type 2 diabetes in male and female Caucasians.
abstract: OBJECTIVE
  Type 2 diabetes mellitus and obesity constitute serious health problems. Studies reveal that the 5-HT2C receptor contributes substantially to the regulation of a wide variety of behavioral and physiological processes including feeding and glucose homeostasis. Our aim was to determine the possible association of the -759C/T polymorphism of the 5-HT2C receptor gene with type 2 diabetes and obesity in male and female individuals Caucasian origin.
  
  
  METHODS
  The study population consisted of 151 patients diagnosed with type 2 diabetes and 164 nondiabetic patients, all of Greek origin. Genomic DNA was extracted from peripheral blood and analyzed for the -759C/T polymorphism of the 5-HT2C receptor gene using polymerase chain reaction-restriction fragment length polymorphism method.
  
  
  RESULTS
  The frequency of T allele of the -759C/T polymorphism of the 5-HT2C receptor was significantly lower in the diabetic group (12.6%) than in the nondiabetic group (23.3%) (P=0.003). The genetic risk of type 2 diabetes for patients not carrying the T allele was increased compared with nondiabetic patients (odds ratio (OR)=2.34, 95% confidence interval (CI)=1.36-4.02). The reduced frequency of T allele was present both in male [10% in patients with diabetes and 21.6% in patients without diabetes (P=0.041)] and female patients [14.1% in patients with diabetes and 24.3% in patients without diabetes (P=0.030)]. In contrast, the frequency of T allele was similar in obese (16.4%) and nonobese (17.1%) patients.
  
  
  CONCLUSIONS
  Lower frequency of -759T allele of the 5- HT2C receptor gene was associated with type 2 diabetes but not with obesity in male and female Caucasians. Thus, this polymorphism might constitute a prognostic marker for diabetic risk.
authors.affiliation: Laboratory of Pharmacology and Clinical Pharmacology, Medical School, Democritus University of Thrace, and Outpatient Diabetic Medical Clinic, Department of Internal Medicine, Academic General Hospital of Alexandroupolis, Alexandroupolis, Thrace, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Iordanidou
authors.forename: Anna
authors.initials: A
authors.lastname: Tavridou
authors.forename: Michalis V
authors.initials: MV
authors.lastname: Vasiliadis
authors.forename: Kostas I
authors.initials: KI
authors.lastname: Arvanitidis
authors.forename: John
authors.initials: J
authors.lastname: Petridis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Christakidis
authors.forename: Vassilios
authors.initials: V
authors.lastname: Vargemezis
authors.forename: George
authors.initials: G
authors.lastname: Bougioukas
authors.forename: Vangelis G
authors.initials: VG
authors.lastname: Manolopoulos
mesh_terms: D000368:Aged; D001483:Base Sequence; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D044405:Receptor, Serotonin, 5-HT2C / Q000235:genetics*; D044465:White People / Q000235:genetics*
chemical_list: D017931:DNA Primers; D044405:Receptor, Serotonin, 5-HT2C