Effect of static pressure on intracellular pH of adhesive Chinese hamster ovary cells.
The effect of static pressure on the intracellular pH of the Chinese hamster ovary (CHO) cell line DR1000L4N was investigated. In cultivation of CHO cells at 0.9 MPa, two distinct populations were observed in the histogram of a flow cytometer, while single population was observed in cultivation at 0.1 MPa. The intracellular pH of the major population at 0.9 MPa was markedly lower than that of the single population at 0.1 MPa.
Fujiwara
Masashi
M
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita 13, Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Koizumi
Satoru
S
Takagi
Mutsumi
M
D000818:Animals; D016466:CHO Cells / Q000737:chemistry* / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008954:Models, Biological; D011312:Pressure