Validated method for the quantification of artepillin-C in Brazilian propolis.
Brazilian propolis contains several phenolic compounds among which 5 diprenyl-4-hydroxycinnamic acid (artepillin-C) is commonly found in areas where flora is rich in Baccharis species. The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and the compound may be used as a chemical marker for quality control in exportating green propolis. This work was to validate the method and evaluate the content of artepillin-C from 33 samples collected in different Brazilian regions. The method used was HPLC with UV-vis detection and a reversed-phase C(18) column. The validation parameters studied were: linearity, accuracy, precision, quantification and detection limits. The results obtained were: detection limit = 0.0036 microg/mL, quantification limit = 0.012 microg/mL, accuracy = 0.0064 and 0.078, recovery 98-102%. Artepillin-C content varied from 0 to 11% depending on the geographical origin. Propolis from the southeast region presented the highest level of artepillin-C (5.0-11.0%). Whist that from the northeast region did not show any artepillin-C.
Matsuda
Adriana Hitomi
AH
Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, Brazil.
de Almeida-Muradian
Ligia Bicudo
LB
D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D010666:Phenylpropionates / Q000032:analysis* / Q000737:chemistry; D011429:Propolis / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D010666:Phenylpropionates; C102259:artepillin C; D011429:Propolis