TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress.
TRP-2 (dopachrome tautomerase) is a melanogenic enzyme whose expression was recently reported to modulate melanocyte response to different cytotoxic events. Here we studied a possible role of TRP-2 in the oxidative stress response in the amelanotic WM35 melanoma cell line. Cell viability assays showed that TRP-2 overexpression in WM35 cells reduced their sensitivity to oxidative stress. Comet assays linked TRP-2 expression to DNA damage protection, and high-performance liquid chromotography-tandem mass spectrometry experiments showed an increase in intracellular glutathione in TRP-2-overexpressing cells. These effects were specifically reversed when TRP-2 was silenced by RNA interference. Nevertheless, these properties appeared to depend on a particular cell environment because expression of TRP-2 failed to rescue HEK epithelial cells exposed to similar treatments.
Michard
Quentin
Q
L'OREAL Recherche, 90 rue du Général Roguet, 92583 Clichy Cedex, France.
Commo
Stephane
S
Belaidi
Jean-Philippe
JP
Alleaume
Anne-Marie
AM
Michelet
Jean-Francois
JF
Daronnat
Edwige
E
Eilstein
Joan
J
Duche
Daniel
D
Marrot
Laurent
L
Bernard
Bruno Alain
BA
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay; D004249:DNA Damage / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism*; D005978:Glutathione; D006801:Humans; D007150:Immunohistochemistry; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D034622:RNA Interference; D053719:Tandem Mass Spectrometry
D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase; D005978:Glutathione