TSOL18/HP6-Tsol, an immunogenic Taenia solium oncospheral adhesion protein and potential protective antigen.
In this study, we employed Taenia solium mRNA extracted from a tapeworm of Venezuelan origin to clone express and test the recombinant protein of the T. solium homologue of the 18-kDa oncospheral adhesion molecule of Taenia saginata (HP6-Tsag/TSA18). We first confirm the conserved nature of the sequence of the T. solium homologue (TSOL18/HP6-Tsol) and demonstrate that the recombinant protein, which, as with its T. saginata homologue, is characterised by a fibronectin type III homology region, functions as an adhesion molecule. This emphasises the possible importance of TSOL18/HP6-Tsol in tissue invasion, thus providing a rational explanation for its efficacy as a vaccine. As protection against Taenia spp., oncospheres is antibody mediated, logically, therefore, TSOL18/HP6-Tsol may also serve as a diagnostic antigen, as is indeed the case for recombinant HP6-Tsag/TSA18.
Parkhouse
R Michael E
RM
Instituto Gulbenkian de Ciencia, Rua Quinta Grande 6, Apartado 14, P-2780-156 Oeiras Codex, Portugal. parkhous@igc.gulbenkian.pt
Bonay
Pedro
P
González
Luis Miguel
LM
Ferrer
Elizabeth
E
Gárate
Teresa
T
Aguilar
Cruz M
CM
Cortez A
Milagros M
MM
Harrison
Leslie J S
LJ
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019556:COS Cells; D002448:Cell Adhesion; D002522:Chlorocebus aethiops; D006801:Humans; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D041201:Taenia solium / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C464926:HP6 protein, Taenia saginata; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C113407:TSOL-18 antigen