Molecular cloning and expression analysis of a novel CONSTANS-like gene from potato.
A full-length cDNA of a StCONSTANS-like (StCOL) gene was cloned from potato (Solanum tuberosum L.) by RT-PCR and RACE. The predicted amino acid sequence of this cDNA has a high degree of identity with other homologous members of the CO or COL family. Analysis of mRNA levels for StCOL shows that it is highly expressed in leaves and becomes weaker during tuberization; moreover, is independent of gibberellin A(3) and sucrose.
Guo
J L
JL
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Yang
Q
Q
Liang
F
F
Xing
Y J
YJ
Wang
Z
Z
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D035281:Plant Tubers / Q000254:growth & development / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology
D017931:DNA Primers; D005875:Gibberellins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D013395:Sucrose; C007842:gibberellic acid