Wide varieties of cationic nanoparticles induce defects in supported lipid bilayers.
Nanoparticles with widely varying physical properties and origins (spherical versus irregular, synthetic versus biological, organic versus inorganic, flexible versus rigid, small versus large) have been previously noted to translocate across the cell plasma membrane. We have employed atomic force microscopy to determine if the physical disruption of lipid membranes, formation of holes and/or thinned regions, is a common mechanism of interaction between these nanoparticles and lipids. It was found that a wide variety of nanoparticles, including a cell penetrating peptide (MSI-78), a protein (TAT), polycationic polymers (PAMAM dendrimers, pentanol-core PAMAM dendrons, polyethyleneimine, and diethylaminoethyl-dextran), and two inorganic particles (Au-NH2, SiO2-NH2), can induce disruption, including the formation of holes, membrane thinning, and/or membrane erosion, in supported lipid bilayers.
Leroueil
Pascale R
PR
Department of Chemistry, Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, Michigan 48019, USA.
Berry
Stephanie A
SA
Duthie
Kristen
K
Han
Gang
G
Rotello
Vincent M
VM
McNerny
Daniel Q
DQ
Baker
James R
JR
Orr
Bradford G
BG
Holl
Mark M Banaszak
MM
D002412:Cations; D003460:Crystallization / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008560:Membrane Fluidity; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D013499:Surface Properties
D002412:Cations; D008051:Lipid Bilayers; D046911:Macromolecular Substances