www.rnaworkbench.com: A new program for analyzing RNA interference.
RNA interference (RNAi) has become an important tool to study and utilize gene silencing by introducing short interfering RNA (siRNA). In order to predict the most efficient siRNAs, a new software tool, RNA Workbench (RNAWB), has been designed and is freely available (after registration) on http://www.rnaworkbench.com. In addition to the standard selection rules, RNAWB includes the possibility of statistical analyses of the applied selection rules (criteria). The role of RNA secondary structures in the RNA interference process as well as the application of sequence rules are discussed to show the applicability of the software.
Vareková
Radka Svobodová
RS
ANF DATA (Siemens), Herspická 5, 639 00 Brno, Czech Republic.
Bradác
Ivan
I
Plchút
Martin
M
Skrdla
Michal
M
Wacenovsky
Michael
M
Mahr
Helmuth
H
Mayer
Georg
G
Tanner
Herbert
H
Brugger
Hermann
H
Withalm
Josef
J
Lederer
Peter
P
Huber
Heinrich
H
Gierlinger
Gerhard
G
Graf
Ronald
R
Tafer
Hakim
H
Hofacker
Ivo
I
Schuster
Peter
P
Polcík
Martin
M
D000465:Algorithms; D001483:Base Sequence; D018390:Gene Targeting / Q000379:methods*; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software
D034741:RNA, Small Interfering