title: Preparation of biodegradable polymer nanoparticles by miniemulsion technique and their cell interactions.
abstract: The emulsion/solvent evaporation method and miniemulsion technique were combined and applied in the formulation of biodegradable monodisperse nanoparticles at high solid contents using different biocompatible and biodegradable polymers such as poly(L-lactide) (PLLA), poly[(D,L-lactide)-co-glycolide] 50:50 (PLGA), and poly(epsilon-caprolactone) (PCL). Differences between the results of various polymers are found in terms of the particle size and size distribution as well as in the degradation time. An encapsulated hydrophobic fluorescent dye was used as a model marker in order to study the entrapment efficiency and diffusion yield out of the particle. Cellular uptake of the obtained particles was observed in Jurkat and HeLa cells. In the investigated particle size range of 80-200 nm, the surfactant on the particles' surface had a greater influence than the particle size. Uptake kinetics reveals that the PLLA and PCL particles are endocytosed much faster than polystyrene particles.
authors.affiliation: Institute of Organic Chemistry III - Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany. anna.musyanovych@uni-ulm.de
authors.forename: Anna
authors.initials: A
authors.lastname: Musyanovych
authors.forename: Julia
authors.initials: J
authors.lastname: Schmitz-Wienke
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
authors.forename: Paul
authors.initials: P
authors.lastname: Walther
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
mesh_terms: D004337:Drug Carriers; D004655:Emulsions / Q000737:chemistry; D004705:Endocytosis / Q000502:physiology*; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D004655:Emulsions; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide)