title: Cleavage of high-molecular-weight kininogen by elastase and tryptase is inhibited by ferritin.
abstract: Ferritin is a protein principally known for its role in iron storage. We have previously shown that ferritin can bind high-molecular-weight kininogen (HK). Upon proteolytic cleavage by the protease kallikrein, HK releases the proinflammatory peptide bradykinin (BK) and other biologically active products, such as two-chain high-molecular-weight kininogen, HKa. At inflammatory sites, HK is oxidized, which renders it a poor substrate for kallikrein. However, oxidized HK remains a good substrate for elastase and tryptase, thereby providing an alternative cleavage mechanism for HK during inflammation. Here we report that ferritin can retard the cleavage of both native HK and oxidized HK by elastase and tryptase. Initial rates of cleavage were reduced 45-75% in the presence of ferritin. Ferritin is not a substrate for elastase or tryptase and does not interfere with the ability of either protease to digest a synthetic substrate, suggesting that ferritin may impede HK cleavage through direct interaction with HK. Immunoprecipitation and solid phase binding studies reveal that ferritin and HK bind directly with a Kd of 134 nM. To test whether ferritin regulates HK cleavage in vivo, we used THP-1 cells, a human monocyte/macrophage cell line that has been used to model pulmonary inflammatory cells. We observed that ferritin impedes the cleavage of HK by secretory proteases in stimulated macrophages. Furthermore, ferritin, HK, and elastase are all present in or on alveolar macrophages in a mouse model of pulmonary inflammation. Collectively, these results implicate ferritin in the modulation of HK cleavage at sites of inflammation.
authors.affiliation: Program in Molecular Medicine, Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
authors.forename: Lan G
authors.initials: LG
authors.lastname: Coffman
authors.forename: Julie C
authors.initials: JC
authors.lastname: Brown
authors.forename: David A
authors.initials: DA
authors.lastname: Johnson
authors.forename: Narayanan
authors.initials: N
authors.lastname: Parthasarathy
authors.forename: Ralph B
authors.initials: RB
authors.lastname: D'Agostino
authors.forename: Mark O
authors.initials: MO
authors.lastname: Lively
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Hua
authors.forename: Stephen L
authors.initials: SL
authors.lastname: Tilley
authors.forename: Werner
authors.initials: W
authors.lastname: Muller-Esterl
authors.forename: Mark C
authors.initials: MC
authors.lastname: Willingham
authors.forename: Frank M
authors.initials: FM
authors.lastname: Torti
authors.forename: Suzy V
authors.initials: SV
authors.lastname: Torti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D005293:Ferritins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D047468:Immunoprecipitation; D007249:Inflammation / Q000503:physiopathology; D019679:Kininogen, High-Molecular-Weight / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D011485:Protein Binding; D013388:Succinimides / Q000737:chemistry; D053802:Tryptases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
chemical_list: D019679:Kininogen, High-Molecular-Weight; D013388:Succinimides; C015752:N-chlorosuccinimide; D005293:Ferritins; D009195:Peroxidase; D010196:Pancreatic Elastase; D053802:Tryptases; D015032:Zinc