A new irreversible enzyme-aided esterification method in organic solvents.
A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reaction. The flavour esters isobutyl acetate, methyl butyrate, ethyl butyrate and benzyl butyrate were synthesized either in small scale (0.05 mol) or large scale (1 mol). The yields range from 82 to 92% within 24 h at 52 degrees C. Optimal molar ratios of reactants were 1:1:1 (carboxylic acid:alcohol:acetoacetate).
Jeromin
Günter E
GE
Aachen University of Applied Sciences, Campus Jülich, FB 3, Ginsterweg 1, 52428, Julich, Germany. jeromin@fh-aachen.de
Zoor
Annegreth
A
D002175:Candida / Q000201:enzymology; D002264:Carboxylic Acids / Q000378:metabolism*; D002384:Catalysis; D002849:Chromatography, Gas; D004800:Enzymes, Immobilized / Q000378:metabolism; D004951:Esterification; D004952:Esters / Q000737:chemistry*; D005656:Fungal Proteins; D008049:Lipase / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D002264:Carboxylic Acids; D004800:Enzymes, Immobilized; D004952:Esters; D005656:Fungal Proteins; D012997:Solvents; D014867:Water; C093667:Novozyme 435; D008049:Lipase