title: Cytomegalovirus strain diversity in seropositive women.
abstract: Infection and reinfection with multiple cytomegalovirus (CMV) strains have been shown to occur in immunocompromised individuals, sexually transmitted disease clinic attendees, and children attending day care centers. To characterize the CMV diversity in healthy seropositive individuals, 16 CMV PCR-positive specimens from 113 seropositive women were analyzed for glycoprotein gN and gB genotypes by cloning, followed by nucleotide sequencing of the plasmid DNA and/or restriction fragment length polymorphism (RFLP). The results showed that most (93.7%) of the PCR-positive specimens contained multiple gN and/or gB genomic variants, suggesting that the majority of women were infected with more than one virus strain. The results also showed that the RFLP technique might not be sufficiently sensitive to detect all of the genomic variants present in a sample.
authors.affiliation: Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, Alabama, USA. zdnovak@peds.uab.edu
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Novak
authors.forename: Shannon A
authors.initials: SA
authors.lastname: Ross
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Patro
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Pati
authors.forename: Rekha A
authors.initials: RA
authors.lastname: Kumbla
authors.forename: Sallie
authors.initials: S
authors.lastname: Brice
authors.forename: Suresh B
authors.initials: SB
authors.lastname: Boppana
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis / Q000097:blood / Q000652:urine; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C417883:glycoprotein N, Human cytomegalovirus