title: Antigenic and genomic characterization of adenovirus associated to respiratory infections in children living in Northeast Brazil.
abstract: From January to December 1998, nasopharyngeal aspirates were obtained from 482 children with acute respiratory infections attended in emergence department and wards of a teaching hospital in the city of Salvador, Brazil. The samples were tested for the presence of adenovirus by isolation in tissue culture and indirect immunofluorescence assay. Eleven adenoviruses were detected by both methods in the same clinical samples. Infections by adenovirus were observed during seven months of the year without association with rainy season. Genome analysis was performed on these 11 isolates. Species C was represented by serotypes 1, 2 and 5. Within species B, only serotype 7 (Ad7) was detected. Two genomic variants of Ad1, two variants of Ad2, one of Ad5, and one of Ad7 (7h) were identified. This is the first study of molecular epidemiology of adenovirus associated to acute respiratory infections in children living in Northeast Brazil, and contributes to a better understanding of adenovirus infections in the country.
authors.affiliation: Laboratório de Virologia, Programa de Pós-Graduação em Microbiologia Médica, Departamento de Patologia e Medicina Legal, Universidade Federal do Ceará, 60441-750 Fortaleza, CE, Brasil. fernandaedna@terra.com.br
authors.forename: Fernanda E A
authors.initials: FE
authors.lastname: Moura
authors.forename: Jacó R L de
authors.initials: JR
authors.lastname: Mesquita
authors.forename: Silvana A R
authors.initials: SA
authors.lastname: Portes
authors.forename: Eduardo A G
authors.initials: EA
authors.lastname: Ramos
authors.forename: Marilda M
authors.initials: MM
authors.lastname: Siqueira
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001938:Brazil / Q000453:epidemiology; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect; D005838:Genotype; D006801:Humans; D009305:Nasopharynx / Q000821:virology; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000821:virology*
chemical_list: D004279:DNA, Viral