title: Split-root study of autoregulation of nodulation in the model legume Lotus japonicus.
abstract: We used a split-root system to determine the timing for induction of the autoregulation of nodulation (AUT) in Lotus japonicus (Regel) Larsen after inoculation with Mesorhizobium loti. The signal took at least five days for full induction of AUT and inhibition of infection thread formation. Strain ML108 (able to nodulate but unable to fix nitrogen) induced full AUT, but ML101 (unable to nodulate or to fix nitrogen) did not induce autoregulation. These results indicate that Nod factor-producing strains induce AUT, but that the nitrogen fixed by rhizobia and supplied to the plant as ammonia does not elicit the AUT in L. japonicus.
authors.affiliation: Department of Environmental Sciences, Faculty of Agriculture, Saga University, 1 Honjyo-machi, Saga, 840-8502, Japan. azuki@cc.saga-u.ac.jp
authors.forename: Akihiro
authors.initials: A
authors.lastname: Suzuki
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Hara
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Kinoue
authors.forename: Mikiko
authors.initials: M
authors.lastname: Abe
authors.forename: Toshiki
authors.initials: T
authors.lastname: Uchiumi
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kucho
authors.forename: Shiro
authors.initials: S
authors.lastname: Higashi
authors.forename: Ann M
authors.initials: AM
authors.lastname: Hirsch
authors.forename: Susumu
authors.initials: S
authors.lastname: Arima
mesh_terms: D006706:Homeostasis; D000070116:Lotus / Q000382:microbiology / Q000502:physiology*; D055170:Plant Root Nodulation; D018517:Plant Roots / Q000382:microbiology / Q000502:physiology; D013997:Time Factors