title: Characterization of neuropeptide F-like immunoreactivity in the blood-feeding hemipteran, Rhodnius prolixus.
abstract: The invertebrate neuropeptide Y (NPY) homolog, neuropeptide F (NPF), has been characterized for a wide range of invertebrate phyla, including platyhelminthes, molluscs, and arthropods. Current hypotheses suggest that NPF may be capable of regulating responses to diverse external cues related to nutritional status and feeding. The qualitative and quantitative distribution of an NPF-like peptide in fifth instar Rhodnius prolixus was undertaken using an antiserum raised against Drosophila NPF. Immunohistochemistry reveals NPF-like immunoreactive neurons and processes in the central nervous system, stomatogastric nervous system and peripheral nervous system. The distribution of NPF-like immunoreactivity within the medial neurosecretory cells of the brain and neurohemal areas of the corpus cardiacum and dorsal vessel, suggests NPF may act as a neurohormone. Immunoreactive processes are present over the surface of the hindgut and the immunoreactivity in these processes is greatly reduced in intensity 24h post-feeding. The quantification of partially purified NPF-like material in the CNS of R. prolixus was conducted by HPLC fractionation and radioimmunoassay. The results suggest that NPF-like material is present in fifth instar R. prolixus and likely released into the hemolymph following a blood meal.
authors.affiliation: Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road, Mississauga, Ont., Canada L5L 1C6. ron.gonzalez@utoronto.ca
authors.forename: Ronald
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Ian
authors.initials: I
authors.lastname: Orchard
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005247:Feeding Behavior; D006458:Hemolymph / Q000378:metabolism; D007150:Immunohistochemistry; D007301:Insect Hormones / Q000032:analysis* / Q000276:immunology*; D009479:Neuropeptides / Q000032:analysis* / Q000276:immunology*; D012239:Rhodnius / Q000737:chemistry* / Q000276:immunology / Q000502:physiology
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; C076101:neuropeptide F