title: Mycobacterial T cell responses in HIV-infected patients with advanced immunosuppression.
abstract: Antimycobacterial T cell reactivity at different stages of HIV infection was investigated. Subjects were screened with purified protein derivative (PPD), early secreted antigenic target (ESAT)-6, and culture filtrate protein (CFP)-10 antigens for interferon (IFN)-gamma-producing effector T cell responses by direct ex vivo enzyme-linked immunospot (ELISpot) assay. The proportion of responders to PPD tuberculin decreased with a reduction in CD4 T cell count, whereas the proportion of responder to ESAT-6 and CFP-10 did not. The main sources of IFN-gamma secretion were CD4 cells, and the relative responses to ESAT-6 and CFP-10 significantly increased in HIV-infected patients with decreasing CD4 cell count. This may reflect early signs of reactivation, reinfection, or a restricted, inefficient immune response to Mycobacterium tuberculosis.
authors.affiliation: Bacterial and Viral Diseases Programme, Medical Research Council Laboratories, Fajara, Banjul, The Gambia, West Africa. ahammond@mrc.gm
authors.forename: Abdulrahman S
authors.initials: AS
authors.lastname: Hammond
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: McConkey
authors.forename: Philip C
authors.initials: PC
authors.lastname: Hill
authors.forename: Sarah
authors.initials: S
authors.lastname: Crozier
authors.forename: Michèl R
authors.initials: MR
authors.lastname: Klein
authors.forename: Richard A
authors.initials: RA
authors.lastname: Adegbola
authors.forename: Sarah
authors.initials: S
authors.lastname: Rowland-Jones
authors.forename: Roger H
authors.initials: RH
authors.lastname: Brookes
authors.forename: Hilton
authors.initials: H
authors.lastname: Whittle
authors.forename: Assan
authors.initials: A
authors.lastname: Jaye
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000276:immunology / Q000382:microbiology; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015658:HIV Infections / Q000150:complications* / Q000276:immunology* / Q000821:virology; D015497:HIV-1; D015498:HIV-2; D006801:Humans; D007165:Immunosuppression Therapy; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D014376:Tuberculosis / Q000150:complications / Q000276:immunology / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C117940:CFP-10 protein, Mycobacterium tuberculosis; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D014373:Tuberculin; D007371:Interferon-gamma