Genetic characterization and authentication of Embelia ribes using RAPD-PCR and SCAR marker.
Embelia ribes Burm. f. (Myrsinaceae) is one of the important plants used in Indian traditional medicine. RAPD-PCR analysis was performed to obtain species-specific DNA fragments. A band of 906 bp that was specific to Embelia ribes irrespective of the geographical source was obtained using the random decamer primer OPF 05. SCAR primers ER 1 (27 mer) and ER 2 (26 mer) were designed from the sequence of the RAPD marker which yielded an expected amplicon of 594 bp with the Embelia ribes DNA only. This methodology can be used for species identification of genuine Embelia ribes and to distinguish it from common substitutes and adulterants. BLAST: basic local alignment search tool ER 1: Embelia ribes forward primer ER 2: Embelia ribes reverse primer RAPD-PCR: random amplification of polymorphic DNA polymerase chain reaction SCAR: sequence characterized amplified region.
Devaiah
K M
KM
Centre for Pharmacognosy & Product Development of Traditional Medicine, Foundation for Revitalization of Local Health Traditions (FRLHT), Bangalore, India.
Venkatasubramanian
Padma
P
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D031645:Embelia / Q000235:genetics*; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D005819:Genetic Markers