title: Apoptotic and necrotic influence of dental resin polymerization initiators in human gingival fibroblast cultures.
abstract: The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators--namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)--on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 microM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G0/G1, S, G2/M phases). All four dental resin polymerization initiators induced G0/G1 cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.
authors.affiliation: Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Masuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nomura
authors.forename: Ujjal Kumar
authors.initials: UK
authors.lastname: Bhawal
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sawajiri
authors.forename: Isao
authors.initials: I
authors.lastname: Hirata
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Nahara
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okazaki
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D001585:Benzoyl Peroxide; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D003188:Composite Resins / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008689:Methacrylates; D009336:Necrosis; D044367:Phase Transition; D017319:Photosensitizing Agents / Q000633:toxicity*; D019163:Reducing Agents / Q000633:toxicity*; D013729:Terpenes; D014052:Toluidines
chemical_list: D017304:Annexin A5; D003188:Composite Resins; D005456:Fluorescent Dyes; D008689:Methacrylates; D017319:Photosensitizing Agents; D019163:Reducing Agents; D013729:Terpenes; D014052:Toluidines; C048339:camphoroquinone; D016650:Fluorescein-5-isothiocyanate; C049840:2-(dimethylamino)ethyl methacrylate; C015835:dimethyl-4-toluidine; D001585:Benzoyl Peroxide