SP1 protein-based nanostructures and arrays.
Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.
Medalsy
Izhar
I
Physical Chemistry Department and Center for Nanoscience and Nanotechnology, The Hebrew University, Jerusalem 91904, Israel.
Dgany
Or
O
Sowwan
Mukhles
M
Cohen
Hezy
H
Yukashevska
Alevtyna
A
Wolf
Sharon G
SG
Wolf
Amnon
A
Koster
Abraham
A
Almog
Orna
O
Marton
Ira
I
Pouny
Yehonathan
Y
Altman
Arie
A
Shoseyov
Oded
O
Porath
Danny
D
D000327:Adsorption; D003460:Crystallization / Q000379:methods*; D007136:Immunoglobulins / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D013499:Surface Properties
D007136:Immunoglobulins; D046911:Macromolecular Substances; C090698:SP1 antigen