Effects of ebselen on glutathione level in neurons exposed to arachidonic acid and 4-hydroxynonenal during simulated ischemia in vitro.
Release of arachidonic acid (AA) is a neurotoxic mechanism of oxidative nature in trauma and ischemia. We studied ebselen effects on AA and 4-hydroxynonenal toxicity in rat cortical neurons in simulated ischemia in vitro by assessing cell viability and glutathione concentration. Ebselen normalized neuronal viability diminished by AA and 4-hydroxynonenal and increased glutathione level in normoxia and ischemia. AA elevated the glutathione level by 52 and 14% and 4-hydroxynonenal by 63 and 39% in normoxia and ischemia, respectively. Ebselen augmented the effect of AA in normoxia and diminished it in ischemia. Effect of 4-hydroxynonenal was similarly modulated by ebselen.
Pawlas
Natalia
N
Department of Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland.
Małecki
Andrzej
A
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D002545:Brain Ischemia / Q000378:metabolism; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005978:Glutathione / Q000378:metabolism*; D054833:Isoindoles; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats
D000447:Aldehydes; D001393:Azoles; D054833:Isoindoles; D018696:Neuroprotective Agents; D016566:Organoselenium Compounds; D016718:Arachidonic Acid; C042986:ebselen; D005978:Glutathione; C027576:4-hydroxy-2-nonenal