title: A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16.
abstract: Previous research suggested that Pseudomonas spp. may attack the pyrrolidine ring of nicotine in a way similar to mammalian metabolism, resulting in the formation of pseudooxynicotine, the direct precursor of a potent tobacco-specific lung carcinogen. In addition, the subsequent intermediates, 6-hydroxy-3-succinoylpyridine (HSP) and 2,5-dihydroxypyridine (DHP) in the Pseudomonas nicotine degradation pathway are two important precursors for drug syntheses. However, there is little information on the molecular mechanism for nicotine degradation via the pyrrolidine pathway until now. In this study we cloned and sequenced a 4,879-bp gene cluster involved in nicotine degradation. Intermediates N-methylmyosmine, pseudooxynicotine, 3-succinoylpyridine, HSP, and DHP were identified from resting cell reactions of the transformant containing the gene cluster and shown to be identical to those of the pyrrolidine pathway reported in wild-type strain Pseudomonas putida S16. The gene for 6-hydroxy-3-succinoylpyridine hydroxylase (HSP hydroxylase) catalyzing HSP directly to DHP was cloned, sequenced, and expressed in Escherichia coli, and the purified HSP hydroxylase (38 kDa) is NADH dependent. DNA sequence analysis of this 936-bp fragment reveals that the deduced amino acid shows no similarity with any protein of known function.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Tang
authors.forename: Shuning
authors.initials: S
authors.lastname: Wang
authors.forename: Lanying
authors.initials: L
authors.lastname: Ma
authors.forename: Xiangzhou
authors.initials: X
authors.lastname: Meng
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Dake
authors.initials: D
authors.lastname: Zhang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family / Q000235:genetics; D009538:Nicotine / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology*; D011725:Pyridines / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013386:Succinates / Q000378:metabolism*
chemical_list: C507268:6-hydroxy-3-succinoyl-pyridine; D017931:DNA Primers; D011725:Pyridines; D013386:Succinates; D009538:Nicotine; D006899:Mixed Function Oxygenases