Paenibacillus forsythiae sp. nov., a nitrogen-fixing species isolated from rhizosphere soil of Forsythia mira.
A nitrogen-fixing bacterium, designated strain T98(T), was isolated from rhizosphere soil of Forsythia mira. Phylogenetic analysis based on a fragment of the nifH gene and the full-length 16S rRNA gene sequence revealed that strain T98(T) was a member of the genus Paenibacillus. High levels of 16S rRNA gene similarity were found between strain T98(T) and Paenibacillus durus ATCC 35681(T) (97.0 %), Paenibacillus sabinae DSM 17841(T) (98.3 %) and Paenibacillus zanthoxyli DSM 18202(T) (96.8 %). Levels of 16S rRNA gene sequence similarity between strain T98(T) and the type strains of other recognized members of the genus Paenibacillus were below 97.0 %. Levels of DNA-DNA relatedness between strain T98(T) and P. durus ATCC 35681(T), P. sabinae DSM 17841(T) and P. zanthoxyli DSM 18202(T) were 27.6, 30.0 and 32.1 %, respectively. The DNA G+C content of strain T98(T) was 50.4 mol%. The major fatty acids were anteiso-C(15 : 0), C(16 : 0) and iso-C(16 : 0). On the basis of its phenotypic characteristics and levels of DNA-DNA hybridization, strain T98(T) is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus forsythiae sp. nov. is proposed. The type strain is T98(T) (=CCBAU 10203(T) =DSM 17842(T)).
Ma
Yu-Chao
YC
National Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, PR China.
Chen
San-Feng
SF
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D031657:Forsythia / Q000382:microbiology*; D020459:Genes, rRNA; D016937:Gram-Positive Endospore-Forming Rods / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S