title: SP1 protein-based nanostructures and arrays.
abstract: Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.
authors.affiliation: Physical Chemistry Department and Center for Nanoscience and Nanotechnology, The Hebrew University, Jerusalem 91904, Israel.
authors.forename: Izhar
authors.initials: I
authors.lastname: Medalsy
authors.forename: Or
authors.initials: O
authors.lastname: Dgany
authors.forename: Mukhles
authors.initials: M
authors.lastname: Sowwan
authors.forename: Hezy
authors.initials: H
authors.lastname: Cohen
authors.forename: Alevtyna
authors.initials: A
authors.lastname: Yukashevska
authors.forename: Sharon G
authors.initials: SG
authors.lastname: Wolf
authors.forename: Amnon
authors.initials: A
authors.lastname: Wolf
authors.forename: Abraham
authors.initials: A
authors.lastname: Koster
authors.forename: Orna
authors.initials: O
authors.lastname: Almog
authors.forename: Ira
authors.initials: I
authors.lastname: Marton
authors.forename: Yehonathan
authors.initials: Y
authors.lastname: Pouny
authors.forename: Arie
authors.initials: A
authors.lastname: Altman
authors.forename: Oded
authors.initials: O
authors.lastname: Shoseyov
authors.forename: Danny
authors.initials: D
authors.lastname: Porath
mesh_terms: D000327:Adsorption; D003460:Crystallization / Q000379:methods*; D007136:Immunoglobulins / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D007136:Immunoglobulins; D046911:Macromolecular Substances; C090698:SP1 antigen