title: Acid, basic, and neutral peptidases present different profiles in chromophobe renal cell carcinoma and in oncocytoma.
abstract: Renal cell carcinomas (RCCs) are neoplasias with high prevalence and mortality. We previously reported that several peptidases may be involved in the pathophysiology of clear cell renal cell carcinoma (CCRCC). Now, to gain insight into the reasons that lead the various RCC types to behave very differently with regard to aggressiveness and response to anticancer treatments, we analyzed subsets of chromophobe renal cell carcinoma (ChRCC), and renal oncocytoma (RO), a benign tumor; as well as different grades and stages of CCRCCs. Particulate APN, APB, and APA activities were decreased in both ChRCC and RO (tumor vs. nontumor tissues). Interestingly, activities were downregulated in a tumor-type specific way and the intensities of the decreases were stronger in the benign tumor than in the malignant type. Moreover, when two key histopathological parameters for tumor prognosis (high vs. low stage and grade) were analyzed, increases of activity were also observed in several of these cell surface peptidases (APN, APB). Some soluble activities (APB, Asp-AP) were also downregulated in the RCCs. With respect to genetic expression, PSA and APN were in a positive correlation related to their activities in both ChRCC and RO; but not APB, Asp-AP, APA, and PGI. These results may suggest an involvement of several peptidases in the pathophysiology of renal cancer, since they presented different patterns of activity and expression in tumors with different behaviors.
authors.affiliation: Dept. of Physiology, Faculty of Medicine and Dentistry, Univ. of the Basque Country, P.O. Box 699, E-48080 Bilbao, Bizkaia, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Blanco
authors.forename: Gorka
authors.initials: G
authors.lastname: Larrinaga
authors.forename: Itxaro
authors.initials: I
authors.lastname: Pérez
authors.forename: José I
authors.initials: JI
authors.lastname: López
authors.forename: Javier
authors.initials: J
authors.lastname: Gil
authors.forename: Ekaitz
authors.initials: E
authors.lastname: Agirregoitia
authors.forename: Adolfo
authors.initials: A
authors.lastname: Varona
mesh_terms: D018249:Adenoma, Oxyphilic / Q000201:enzymology / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007680:Kidney Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010447:Peptide Hydrolases / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D010447:Peptide Hydrolases