Characterization of interactions between and among components of the meiotic silencing by unpaired DNA machinery in Neurospora crassa using bimolecular fluorescence complementation.
Bimolecular fluorescence complementation (BiFC) is based on the complementation between two nonfluorescent fragments of the yellow fluorescent protein (YFP) when they are united by interactions between proteins covalently linked to them. We have successfully applied BiFC in Neurospora crassa using two genes involved in meiotic silencing by unpaired DNA (MSUD) and observed macromolecular complex formation involving only SAD-1 proteins, only SAD-2 proteins, and mixtures of SAD-1 and SAD-2 proteins.
Bardiya
Nirmala
N
Division of Biological Sciences, University of Missouri, Columbia 65211, USA.
Alexander
William G
WG
Perdue
Tony D
TD
Barry
Edward G
EG
Metzenberg
Robert L
RL
Pukkila
Patricia J
PJ
Shiu
Patrick K T
PK
D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004271:DNA, Fungal / Q000378:metabolism*; D005453:Fluorescence; D005656:Fungal Proteins / Q000378:metabolism; D020868:Gene Silencing; D008163:Luminescent Measurements / Q000379:methods*; D008164:Luminescent Proteins / Q000378:metabolism*; D008540:Meiosis; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000166:cytology* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D004271:DNA, Fungal; D005656:Fungal Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C054562:yellow fluorescent protein, Bacteria