title: Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey.
abstract: Using HPLC a fraction of New Zealand manuka honey has been isolated, which gives rise to the non-peroxide antibacterial activity. This fraction proved to be methylglyoxal, a highly reactive precursor in the formation of advanced glycation endproducts (AGEs). Methylglyoxal concentrations in 49 manuka and 34 non-manuka honey samples were determined using a direct detection method and compared with values obtained using standard o-phenylenediamine derivatisation. Concentrations obtained using both the methods were similar and varied from 38 to 828 mg/kg.
authors.affiliation: Chemistry Department, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Adams
authors.forename: Cherie H
authors.initials: CH
authors.lastname: Boult
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Deadman
authors.forename: Judie M
authors.initials: JM
authors.lastname: Farr
authors.forename: Megan N C
authors.initials: MN
authors.lastname: Grainger
authors.forename: Merilyn
authors.initials: M
authors.lastname: Manley-Harris
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Snow
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006722:Honey / Q000032:analysis*; D031649:Leptospermum / Q000737:chemistry*; D050296:Microbial Viability / Q000187:drug effects; D010545:Peroxides / Q000737:chemistry; D011765:Pyruvaldehyde / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010545:Peroxides; D011765:Pyruvaldehyde