Renal artery injection for delivery of biological materials to the glomerulus.
OBJECTIVE
We investigated whether injection of synthetic small interfering siRNAs via renal artery followed by electroporation could be effective in silencing specific genes in glomerulus.


METHODS
Solution of siRNA targeting enhanced green fluorescent protein (EGFP) was injected via the renal artery of EGFP-transgenic rats followed by delivery of electric pulses.


RESULTS
The delivery of siRNA reduced endogenous EGFP expression, mainly in glomerular mesangial cells.


CONCLUSIONS
Electroporation-mediated gene transfer system via renal artery in rats is very useful for the delivery of a range of different materials including cells, viruses and proteins. Here, we provide a step-by-step description of this perfusion method and a discussion of the key points for the success of the technique.
Takabatake
Yoshitsugu
Y
Department of Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan.
Isaka
Yoshitaka
Y
Imai
Enyu
E
D000818:Animals; D004195:Disease Models, Animal; D018274:Electroporation / Q000379:methods; D005456:Fluorescent Dyes / Q000008:administration & dosage; D018390:Gene Targeting / Q000379:methods; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000096:biosynthesis; D007150:Immunohistochemistry; D007269:Injections, Intra-Arterial; D007674:Kidney Diseases / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D007678:Kidney Glomerulus / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D051381:Rats; D012077:Renal Artery; D014162:Transfection
D005456:Fluorescent Dyes; D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins