title: Growth hormone action in the developing neural retina: a proteomic analysis.
abstract: The possible presence and action of growth hormone (GH) in the neural retina was investigated in newborn mice. The neural retina was found to be a site of GH gene expression, as GH mRNA was abundant in cells of the retinal ganglion cell layer, in which GH was also detected. It was also a site of GH action, since GH receptor (GHR) immunoreactivity mirrored that of GH. Actions of GH within the eye were indicated by a reduction in its axial length and retinal width (its neuroblastic, inner plexiform, and optic fiber layers) in GHR gene disrupted mice (GHR-/-), in comparison with wild type (GHR+/+) littermates. In the absence of GH signaling, four proteins in the retinal proteome of the GHR-/- mice (identified by 2-D gels and MS) differed in abundance with those in the wild type mice. Brain abundant membrane attached signal protein-1 (BASP-1) was down-regulated, whereas protein kinase C inhibitor 1, cyclophilin A, KH domain-containing, RNA-binding, signal transduction-associated protein 3 were up-regulated in GHR-/- mice. These proteins are involved in retinal vascularization, neural proliferation and neurite outgrowth. GH might thus have hitherto unsuspected roles in these processes during retinal development.
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Canada.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Baudet
authors.forename: Zahra
authors.initials: Z
authors.lastname: Hassanali
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Sawicki
authors.forename: Edward O
authors.initials: EO
authors.lastname: List
authors.forename: John J
authors.initials: JJ
authors.lastname: Kopchick
authors.forename: Steve
authors.initials: S
authors.lastname: Harvey
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis; D021984:Cyclophilin A / Q000096:biosynthesis; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D015536:Down-Regulation; D018507:Gene Expression Regulation, Developmental; D013006:Growth Hormone / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000096:biosynthesis; D011986:Receptors, Somatotropin / Q000172:deficiency / Q000502:physiology*; D012160:Retina / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: C489363:Basp1 protein, mouse; D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; C489275:Hint1 protein, mouse; C486528:Khdrbs3 protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011986:Receptors, Somatotropin; D013006:Growth Hormone; D021984:Cyclophilin A