title: Mosquito densonucleosis virus non-structural protein NS2 is necessary for a productive infection.
abstract: Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does NS1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny.
authors.affiliation: Department of Microbiology, Immunology and Pathology, Colorado State University, Ft Collins, CO 80523, USA.
authors.forename: Eugene
authors.initials: E
authors.lastname: Azarkh
authors.forename: Erin
authors.initials: E
authors.lastname: Robinson
authors.forename: Supanee
authors.initials: S
authors.lastname: Hirunkanokpun
authors.forename: Boris
authors.initials: B
authors.lastname: Afanasiev
authors.forename: Pattamaporn
authors.initials: P
authors.lastname: Kittayapong
authors.forename: Jonathan
authors.initials: J
authors.lastname: Carlson
authors.forename: Joe
authors.initials: J
authors.lastname: Corsini
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D009033:Culicidae / Q000821:virology*; D003593:Cytoplasm / Q000737:chemistry; D019223:Densovirinae / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D050296:Microbial Viability / Q000235:genetics; D008856:Microscopy, Fluorescence; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins