Scaffolds based on hyaluronan crosslinked with a polyaminoacid: Novel candidates for tissue engineering application.
New porous scaffolds, with a suitable hydrolytic and enzymatic degradation, useful for tissue engineering applications have been obtained by a carbodiimide mediated reaction between hyaluronan (HA) and a synthetic polymer with a polyaminoacid structure such as alpha,beta-polyaspartylhydrazide (PAHy). Scaffolds with a different molar ratio between PAHy repeating units and HA repeating units have been prepared and characterized from a chemical and physicochemical point of view. Tests of indirect and direct cytotoxicity, cell adhesion, and spreading on these biomaterials have been performed by using murine L929 fibroblasts. The new biomaterials showed a good cell compatibility and ability to allow cell migration into the scaffolds as well as spreading on their surface.
Pitarresi
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Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy. giopitar@unipa.it
Palumbo
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FS
Cavallaro
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Faré
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Giammona
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D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D019245:Compressive Strength; D006820:Hyaluronic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D010455:Peptides / Q000737:chemistry*; D011108:Polymers; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D010455:Peptides; D011108:Polymers; C442699:poly(aspartylhydrazide); D006820:Hyaluronic Acid