title: A possible mechanism of antitumor activity of 5(5',6'benzocoumaroyl-3')-methylaminouracil in vivo.
abstract: OBJECTIVE
  To analyze possible mechanisms of anticancer activity of hydrobromide 5-(5',6'-benzocoumaroyl-3')-methylaminouracil (BCU) in vivo.
  
  
  METHODS
  BCU was administered at the dose of 6 mg/kg for seven days to rats bearing Guerin carcinoma (Gc). Interaction of BCU with DNA isolated from Gc cells was analyzed by electrophoresis and spectrophotometry.
  
  
  RESULTS
  It has been shown that BCU administration resulted in chromatin condensation, nuclei picnosis, increase of the DNase activity and the appearance of high-molecular DNA fragments in Gc cells.
  
  
  CONCLUSIONS
  BCU is shown to be able to interact directly with DNA molecules with the formation of stable complexes.
authors.affiliation: Department of Biochemistry, Y Fedkovych Chernivtsy National University, Chernivtsy, Ukraine.
authors.forename: H P
authors.initials: HP
authors.lastname: Kopylchuk
authors.forename: I O
authors.initials: IO
authors.lastname: Shmarakov
authors.forename: M M
authors.initials: MM
authors.lastname: Marchenko
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects*; D005260:Female; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D051381:Rats; D014498:Uracil / Q000031:analogs & derivatives* / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D014498:Uracil