title: Plasma DNA: a molecular marker of surgical insult and postoperative recovery in esophageal cancer.
abstract: OBJECTIVE
  To assess plasma DNA changes intraoperatively, to relate plasma DNA to the magnitude of the surgical insult and to monitor the changes during the postoperative recovery period.
  
  
  METHODS
  Prospective study of 35 patients with esophageal cancer who had esophagectomy of different magnitudes: 19 esophagectomy without thoracotomy and 16 esophagectomy with thoracotomy. The plasma DNA was measured prior to surgery, throughout the course of the operation on four different intervals, and on postoperative days 1, 3, 5, and 7.
  
  
  RESULTS
  A significant difference was seen in the median plasma DNA intraoperatively between the two groups: esophagectomy without thoracotomy, 507 ng/ml/min (range 211-2,708), esophagectomy with thoracotomy, median 1,098 ng/ml/min (range 295-22,284; p = 0.014). Postoperative complications were identified in 6 patients who demonstrated a significant elevation in plasma DNA on postoperative days 5 and 7.
  
  
  CONCLUSIONS
  Plasma DNA increases during surgery as a result of cell damage and the rise correlates with the magnitude of surgery. The descent of plasma DNA postoperatively correlates with surgical recovery. Elevation of the plasma DNA during the postoperative period correlates with postoperative complications. Plasma DNA is an objective molecular marker of surgical insult and can be used to monitor postoperative recovery after esophagectomy.
authors.affiliation: Department of Surgery, University of California, Keck School of Medicine, Los Angeles, Calif., USA. fbanki@surgery.usc.edu
authors.forename: F
authors.initials: F
authors.lastname: Banki
authors.forename: R J
authors.initials: RJ
authors.lastname: Mason
authors.forename: J A
authors.initials: JA
authors.lastname: Hagen
authors.forename: S R
authors.initials: SR
authors.lastname: Demeester
authors.forename: J C
authors.initials: JC
authors.lastname: Lipham
authors.forename: K
authors.initials: K
authors.lastname: Danenberg
authors.forename: P
authors.initials: P
authors.lastname: Danenberg
authors.forename: T R
authors.initials: TR
authors.lastname: Demeester
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor; D004247:DNA / Q000097:blood*; D004938:Esophageal Neoplasms / Q000097:blood* / Q000601:surgery; D016629:Esophagectomy / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011183:Postoperative Complications / Q000097:blood / Q000534:rehabilitation; D011184:Postoperative Period; D013908:Thoracotomy / Q000009:adverse effects*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; D004247:DNA