Effect of retinol palmitate eye drops on experimental keratoconjunctival epithelial damage induced by n-heptanol in rabbit.
OBJECTIVE
To investigate ability of vitamin A (VA, retinol palmitate) to promote wound healing after injury in rabbit eye.


METHODS
After keratoconjunctival injury by 20 microL of n-heptanol, VA eye drops at 500, 1000, or 1500 IU/mL were started 6 times a day for 11 days. Fluorescein and rose bengal staining and histological analysis were performed.


RESULTS
Fluorescein staining was significantly reduced by 1000 and 1500 IU/mL of VA and rose bengal staining by all concentrations. Histological examination revealed acceleration of wound healing after 7 days (1500 IU/mL).


CONCLUSIONS
VA may be useful in treatment of keratoconjunctival injury.
Toshida
Hiroshi
H
Department of Ophthalmology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan. toshida@med.juntendo.ac.jp
Odaka
Akito
A
Koike
Daisuke
D
Murakami
Akira
A
D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy*; D003229:Conjunctival Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003316:Corneal Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D004224:Diterpenes; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005126:Eye Burns / Q000139:chemically induced*; D005136:Eye Proteins / Q000378:metabolism; D019793:Fluorescein; D005456:Fluorescent Dyes; D019850:Heptanol / Q000633:toxicity; D008297:Male; D009077:Mucins / Q000378:metabolism; D009883:Ophthalmic Solutions / Q000008:administration & dosage* / Q000627:therapeutic use; D011817:Rabbits; D000084562:Retinyl Esters; D012395:Rose Bengal; D013666:Tears / Q000378:metabolism; D014801:Vitamin A / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
D004224:Diterpenes; D005136:Eye Proteins; D005456:Fluorescent Dyes; D009077:Mucins; D009883:Ophthalmic Solutions; D000084562:Retinyl Esters; C042434:tear proteins; D014801:Vitamin A; C014794:retinol palmitate; D012395:Rose Bengal; D019850:Heptanol; D019793:Fluorescein