Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.
Pro- and mature BDNF activate very different receptors and intracellular pathways, potentially leading to either neuronal death or survival. Here we examined the biochemistry of endogenous BDNF in mouse neurons using sensitive reagents and found that pro-BDNF is rapidly converted intracellularly to mature BDNF, the latter being stored and released by excitatory input.
Matsumoto
Tomoya
T
Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland.
Rauskolb
Stefanie
S
Polack
Martin
M
Klose
Johannes
J
Kolbeck
Roland
R
Korte
Martin
M
Barde
Yves-Alain
YA
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000172:deficiency / Q000378:metabolism*; D019556:COS Cells; D002522:Chlorocebus aethiops; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006624:Hippocampus / Q000166:cytology*; D047468:Immunoprecipitation / Q000379:methods; D017774:Long-Term Potentiation / Q000502:physiology / Q000528:radiation effects; D008715:Methionine / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011498:Protein Precursors / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology / Q000528:radiation effects; D014162:Transfection / Q000379:methods; D014316:Tritium / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D011498:Protein Precursors; C426674:brain-derived neurotrophic factor precursor; D014316:Tritium; D008715:Methionine