title: Ym1 and Ym2 expression in a mouse model exposed to diesel exhaust particles.
abstract: BACKGROUND
  Chitinase may play a role in regulating allergic diseases.
  
  
  OBJECTIVE
  We studied the role of chitinase in a mouse model exposed to diesel exhaust particles (DEP). Mice were exposed to intranasal DEP (0.6 mg/mL) for 5 days and challenged with aerosolized DEP (6 mg/m(3)) on days 6-8. Enhanced pause (Penh), as an airway obstruction marker, was measured on day 9, and bronchoalveolar lavage (BAL) fluid and lung tissues were collected on day 10. The expression of Ym1 and Ym2 mRNA was assessed in lung tissue extracts by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  DEP induced significant increases in methacholine-induced Penh and IL-4 levels in BAL fluid relative to the control group. Peribronchial and perivascular inflammatory cell infiltrates were prominent in the DEP group. DEP induced Ym1 and Ym2 mRNA expression in lung tissue extracts relative to the control group.
  
  
  CONCLUSIONS
  These results demonstrate that DEP induced airway hyperresponsiveness and Ym mRNA expression via a Th2 cell-biased response, suggesting that chitinase may play an important role in airway inflammation and responsiveness upon exposure to DEP in a mouse model, and may therefore be involved in regulating allergic diseases.
authors.affiliation: Department of Internal Medicine, Soonchunhyang University Hospital, Bucheon, Korea.
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Song
authors.forename: An-Soo
authors.initials: AS
authors.lastname: Jang
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Ahn
authors.forename: Hajime
authors.initials: H
authors.lastname: Takizawa
authors.forename: Shin-Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Ji-Hee
authors.initials: JH
authors.lastname: Kwon
authors.forename: Young-Mok
authors.initials: YM
authors.lastname: Lee
authors.forename: Tai Youn
authors.initials: TY
authors.lastname: Rhim
authors.forename: Choon-Sik
authors.initials: CS
authors.lastname: Park
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001249:Asthma / Q000139:chemically induced* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002688:Chitinases / Q000235:genetics; D004195:Disease Models, Animal; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D005742:Gasoline / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D019570:Inhalation Exposure; D015847:Interleukin-4 / Q000378:metabolism; D037102:Lectins / Q000235:genetics; D016210:Methacholine Chloride; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D001335:Vehicle Emissions / Q000633:toxicity; D001619:beta-N-Acetylhexosaminidases / Q000235:genetics
chemical_list: D000393:Air Pollutants; D005742:Gasoline; D037102:Lectins; D012333:RNA, Messenger; D001335:Vehicle Emissions; D016210:Methacholine Chloride; D015847:Interleukin-4; D002688:Chitinases; C525260:Ym2 protein, mouse; C429541:Chil3 protein, mouse; D001619:beta-N-Acetylhexosaminidases