title: One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device.
abstract: Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
authors.affiliation: Department of Chemistry, Liverpool University, Liverpool, L69 7ZD, UK.
authors.forename: Jenny
authors.initials: J
authors.lastname: Aveyard
authors.forename: Maryam
authors.initials: M
authors.lastname: Mehrabi
authors.forename: Andrew
authors.initials: A
authors.lastname: Cossins
authors.forename: Helen
authors.initials: H
authors.lastname: Braven
authors.forename: Robert
authors.initials: R
authors.lastname: Wilson
mesh_terms: D003101:Collodion / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D011934:Reagent Strips; D012680:Sensitivity and Specificity
chemical_list: D009696:Nucleic Acids; D009841:Oligonucleotides; D011934:Reagent Strips; D006046:Gold; D003101:Collodion