Thalassobaculum litoreum gen. nov., sp. nov., a member of the family Rhodospirillaceae isolated from coastal seawater.
A Gram-negative, facultatively anaerobic strain with slightly curved and straight rod-shaped cells, strain CL-GR58(T), was isolated from coastal seawater (near Gori, Korea). Analyses of the 16S rRNA gene sequence revealed that strain CL-GR58(T) belonged to the family Rhodospirillaceae with Azospirillum lipoferum as its closest relative (gene sequence similarity of 90.9 %). Phylogenetic analyses of the 16S rRNA gene sequences showed that strain CL-GR58(T) was not associated with any known genera in the family Rhodospirillaceae. The novel strain grew in the presence of 1-10 % sea salts, optimally at 30-35 degrees C and pH 8. The major cellular fatty acids consisted of C(18 : 1)omega7c (48.5 %), C(16 : 0) (14.8 %), C(17 : 0) (12.2 %), C(19 : 0) cyclo omega8c (6.3 %) and summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH, 6.0 %). Among the phylogenetically related genera, the fatty acid C(17 : 0) was found only in strain CL-GR58(T). The DNA G+C content of the novel strain was 68.0 mol%. According to phylogenetic analyses of the 16S rRNA gene sequence, fatty acid content and the physiological data, strain CL-GR58(T) represents a novel species in a new genus of the family Rhodospirillaceae, for which the name Thalassobaculum litoreum gen. nov., sp. nov. is proposed. The type strain of the type species is CL-GR58(T) (=KCCM 42674(T)=DSM 18839(T)).
Zhang
Gwang I
GI
School of Earth and Environmental Sciences and Research Institute of Oceanography, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Hwang
Chung Y
CY
Cho
Byung C
BC
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S