title: Real-time monitoring approach: assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix.
abstract: This paper presents an in situ impedance chip system, which allows studying the effects of drugs on the behaviors of living cell in real-time. A new label-free measurement approach is introduced, which enables to assess the extent of the adhesion of a cell population to the extracellular matrix (ECM)-coated microelectrode array, by adding together all the impedance changes from individually controlled microelectrodes. A high sensitivity was demonstrated and allowed us to monitor cell attachment with the resolution of a "few cells". The dose and pre-incubation time effects of antibodies against beta1-integrin and its subunit alpha2beta1-integrin on the adhesion behavior of NCI-H460 lung cancer cells to collagen type I was extensively studied. Results show that both anti-beta1-integrin and anti-alpha2beta1-integrin inhibit NCI-H460 cells attachment to collagen I. This indicates that beta1-integrin is present on the surface of NCI-H460 cancer cells, and that its subunit alpha2beta1 significantly affects NCI-H460 cells attachment on collagen I.
authors.affiliation: Institute of Microelectronics, 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore. chenyu@ime.a-star.edu.sg
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Juntao
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Reboud
authors.initials: R
authors.lastname: Julien
authors.forename: Kumcheong
authors.initials: K
authors.lastname: Tang
authors.forename: Narayanan
authors.initials: N
authors.lastname: Balasubramanian
mesh_terms: D000906:Antibodies / Q000276:immunology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D017097:Electric Impedance; D005109:Extracellular Matrix / Q000502:physiology*; D006801:Humans; D038982:Integrin alpha2beta1 / Q000502:physiology*; D019012:Integrin beta1 / Q000502:physiology*; D008175:Lung Neoplasms / Q000473:pathology*
chemical_list: D000906:Antibodies; D038982:Integrin alpha2beta1; D019012:Integrin beta1