title: Label-free detection of DNA hybridization at a liquid|liquid interface.
abstract: A novel electrochemical approach for label-free detection of DNA primary sequence has been proposed. The flow of nonelectroactive ions across a liquid|liquid interface was used as an electrochemical probe for detection of DNA hybridization. Disposable graphite screen-printed electrodes shielded with a thin layer of inert polymer plasticized with water-immiscible polar organic solvent were modified by probe oligonucleotide and used as a DNA sensor. The specific DNA coupling has been detected with impedance spectroscopy by decrease of ion-transfer resistance. The detection limit was of 10-8 M of target oligonucleotide. The reported sensor was suitable for discrimination of a single mismatch oligonucleotide from the full complementary one. The reported DNA sensor was advantageous over known physicochemical approaches, providing the most significant changes in the measured parameters.
authors.affiliation: Faculty of Chemistry, M.V. Lomonosov Moscow State University, Lenin's Hills, Moscow, Russia. MYV@enzyme.chem.msu.ru
authors.forename: Mikhail Yu
authors.initials: MY
authors.lastname: Vagin
authors.forename: Stanislav A
authors.initials: SA
authors.lastname: Trashin
authors.forename: Arkady A
authors.initials: AA
authors.lastname: Karyakin
authors.forename: Marco
authors.initials: M
authors.lastname: Mascini
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D006108:Graphite / Q000737:chemistry; D008839:Microelectrodes; D009693:Nucleic Acid Hybridization / Q000379:methods; D009841:Oligonucleotides / Q000032:analysis; D009930:Organic Chemicals / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*; D012997:Solvents / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D009930:Organic Chemicals; D011108:Polymers; D012997:Solvents; D014867:Water; D006108:Graphite; D004247:DNA