Convenient transformation of anamorphic basidiomycetous yeasts belonging to genus pseudozyma induced by electroporation.
A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/mug of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.
Morita
Tomotake
T
Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5-2, 1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Habe
Hiroshi
H
Fukuoka
Tokuma
T
Imura
Tomohiro
T
Kitamoto
Dai
D
D001487:Basidiomycota / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D010957:Plasmids / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics*
D004271:DNA, Fungal