title: Clinical relevance of polymorphonuclear (PMN-) elastase determination in semen and serum during infertility investigation.
abstract: The polymorphonuclear (PMN) elastase is secreted by activated granulocytes and is widely used as a marker of male accessory gland infection. However, the clinical value of routine determination of seminal plasma (SP) PMN elastase in asymptomatic patients during infertility investigation has not clearly been established and not much is known about the significance of PMN-elastase levels in serum as a potential biochemical determinant associated with infection/inflammation of the male genital tract. This prospective study included a total of 221 asymptomatic males from unselected subfertile couples, to evaluate the relationship of (i) serum and (ii) same-day SP PMN elastase concentrations with established semen quality parameters, including sperm functional capacity, local antisperm antibodies (ASA), seminal leucocytes, and the outcome of semen cultures including typical sexually transmitted disease pathogens, and a potential association with patients' medical history and results of clinical andrological examination. Furthermore, couples were followed up for subsequent fertility (controlled for female infertility factors). The concentrations of PMN elastase in serum and in SP were not significantly related to semen quality [with regard to microscopic (e.g. count, motility, morphology) as well as biochemical parameters, and also to local ASA of the IgG- or IgA-class]. There was no strong relationship with sperm functional capacity. No significant relationship with the outcome of the microbial screening was found. PMN-elastase levels in serum and SP were not significantly correlated and there was no association with subsequent fertility. Therefore, the value of routine determination of PMN elastase in semen and/or serum samples, particularly when used as a single parameter to screen for subclinical infection/inflammation in males under infertility investigation is limited.
authors.affiliation: Department of Gynaecological Endocrinology and Reproductive Medicine, Women's Hospital, University of Heidelberg, Heidelberg, Germany. waltraud_eggert-kruse@med.uni-heidelberg.de
authors.forename: W
authors.initials: W
authors.lastname: Eggert-Kruse
authors.forename: K
authors.initials: K
authors.lastname: Zimmermann
authors.forename: W
authors.initials: W
authors.lastname: Geissler
authors.forename: A
authors.initials: A
authors.lastname: Ehrmann
authors.forename: R
authors.initials: R
authors.lastname: Boit
authors.forename: T
authors.initials: T
authors.lastname: Strowitzki
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000097:blood / Q000201:enzymology*; D007958:Leukocyte Count; D019272:Leukocyte Elastase / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D011446:Prospective Studies; D012661:Semen / Q000201:enzymology* / Q000276:immunology / Q000382:microbiology; D055101:Semen Analysis; D013076:Sperm Count; D013081:Sperm Motility; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D019272:Leukocyte Elastase