Determination of serum 7-ketocholesterol concentrations and their relationships with coronary multiple risks in diabetes mellitus.
Oxysterols have cytotoxic effects and contribute to the development of atherosclerosis. To examine association between 7-ketocholesterol and diabetes mellitus, and other coronary risk factors, we developed a reliable quantitative method to measure serum 7-ketocholesterol (s-7KCHO) and studied s-7KCHO in patients with type 2 diabetes mellitus (T2DM). The s-7KCHO was detected by gas chromatography-mass spectrometry assay. The s-7KCHO was significantly higher in patients with T2DM (n=137, 33.8 ng/ml) compared to non-diabetic healthy subjects (n=89, 16.1 ng/ml). Patients with T2DM were divided into two groups with two or more than two risk factors (defined as multiple risk factors group) and with zero or one risk factor (non-multiple risk factors group). The s-7KCHO was significantly higher in multiple risk factors group (39.5 ng/ml) compared to non-multiple risk factors (30.1 ng/ml). Among patients with multiple risk factors group, s-7KCHO was significantly higher in patients with high low-density lipoprotein cholesterol (LDL-C) levels (45.1+/-5.9 ng/ml) compared to those with normal LDL-C levels (35.3+/-7.0 ng/ml). Furthermore, s-7KCHO increased according to the number of concurrent coronary risk factors. These results suggest that serum 7-ketocholesterol levels may depend on the multiple risk factors and serum LDL-C levels.
Endo
Kei
K
The Center of Diabetes, Endocrinology, and Metabolism, Toho University Medical Center Sakura Hospital, 564-1 Shimoshizu, Sakura-City, Chiba, Japan.
Oyama
Tomokazu
T
Saiki
Atsuhito
A
Ban
Noriko
N
Ohira
Masahiro
M
Koide
Nobukiyo
N
Murano
Takeyoshi
T
Watanabe
Hitoshi
H
Nishii
Manabu
M
Miura
Minoru
M
Sekine
Kyoichi
K
Miyashita
Yoh
Y
Shirai
Koji
K
D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D002624:Chemistry, Clinical / Q000295:instrumentation / Q000592:standards; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood* / Q000453:epidemiology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000453:epidemiology; D003925:Diabetic Angiopathies / Q000097:blood* / Q000453:epidemiology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000592:standards*; D006801:Humans; D007653:Ketocholesterols / Q000032:analysis / Q000097:blood*; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000453:epidemiology; D008875:Middle Aged; D015203:Reproducibility of Results; D012307:Risk Factors
D015415:Biomarkers; D008078:Cholesterol, LDL; D007653:Ketocholesterols; C003001:7-ketocholesterol