title: Medium optimization for enhanced production of cytosine-substituted mildiomycin analogue (MIL-C) by Streptoverticillium rimofaciens ZJU 5119.
abstract: Cytosine-substituted mildiomycin analogue (MIL-C) was produced effectively by supplementing cytosine into the culture of Streptoverticillium rimofaciens. In order to improve the yield of MIL-C, statistically-based experimental designs were applied to optimize the fermentation medium for S. rimofaciens ZJU 5119. Fifteen culture conditions were examined for their significances on MIL-C production using Plackett-Burman design. The Plackett-Burman design and one-variable-at-a-time design indicated that glucose and rice meal as the complex carbon sources, and peanut cake meal and NH4NO3 as the complex nitrogen sources were beneficial for MIL-C production in S. rimofaciens ZJU 5119. The results of further central composition design (CCD) showed that the optimal concentration of glucose, rice meal and peanut cake meal were 18.7 g/L, 64.8 g/L and 65.1 g/L, respectively. By using this optimal fermentation medium, the MIL-C concentration was increased up to 1336.5 mg/L, an approximate 3.8-fold improvement over the previous concentration (350.0 mg/L) with un-optimized medium. This work will be very helpful to the large-scale production of MIL-C in the future.
authors.affiliation: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ye
authors.forename: Zhi-nan
authors.initials: ZN
authors.lastname: Xu
authors.forename: Pei-lin
authors.initials: PL
authors.lastname: Cen
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D003596:Cytosine / Q000031:analogs & derivatives / Q000096:biosynthesis; D005285:Fermentation; D013306:Streptomycetaceae / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D003596:Cytosine; C017539:mildiomycin