Grouper Mx confers resistance to nodavirus and interacts with coat protein.
Over-expression of grouper Mx negatively regulated nodavirus activity through direct interaction, likely via the binding and perturbation of the intracellular localization of nodavirus coat protein. Deletion analysis of grouper Mx indicated that the coat protein binds to the effector domain of Mx. The presence of grouper Mx in a poly [I:C] interferon system inhibited nodavirus infection, demonstrating that grouper Mx over-expression has an inhibitory effect on both coat protein and RNA-dependent RNA polymerase of nodavirus antigens, which results in reduced viral yields. We conclude that grouper Mx has a key role in cellular resistance to nodavirus infection.
Chen
Young-Mao
YM
Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
Su
Yung-Lin
YL
Shie
Pei-Shiuan
PS
Huang
Shao-Ling
SL
Yang
Huey-Lang
HL
Chen
Tzong-Yueh
TY
D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism*; D049109:Cell Proliferation; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005399:Fishes / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D005786:Gene Expression Regulation; D030381:Nodaviridae / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
D036022:Capsid Proteins; D029941:Fish Proteins