Polarity in archaeal operon transcription in Thermococcus kodakaraensis.
An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential beta-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.
Santangelo
Thomas J
TJ
Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA.
Cubonová
L'ubomíra
L
Matsumi
Rie
R
Atomi
Haruyuki
H
Imanaka
Tadayuki
T
Reeve
John N
JN
D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D019848:Gene Expression Regulation, Archaeal; D019766:Genes, Archaeal / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009876:Operon / Q000235:genetics*; D019712:Thermococcus / Q000235:genetics*; D014158:Transcription, Genetic
D019843:Archaeal Proteins; D006026:Glycoside Hydrolases