Gas chromatographic separation of fatty acid methyl esters on weakly polar capillary columns.
It was found that weakly polar columns, routinely used in capillary GC for analyzing sterols, food additives, etc., can also be used for separating fatty acid methyl esters (FAMEs). On these columns, FAMEs elute in the order of their unsaturation. The equivalent chain-length value of methyl 22:6 is below 23.00. This means FAMEs within a carbon chain length, having up to six double bonds, elute before the next (one carbon longer) saturated FAME elutes. Peak identification is easy. Weakly polar columns are compatible in both GC and GC/MS systems.
Yamamoto
Kouhei
K
Department of Clinical Nutrition, Osaka Prefecture University, 7-30 Habikino 3-chome, Habikino 583-8555, Japan.
Kinoshita
Akemi
A
Shibahara
Akira
A
D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D004952:Esters; D005227:Fatty Acids / Q000737:chemistry* / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry
D004952:Esters; D005227:Fatty Acids