title: Antimicrobial effects of photodynamic therapy on patients with necrotic pulps and periapical lesion.
abstract: This study analyzed the antimicrobial effect of photodynamic therapy (PDT) in association with endodontic treatment. Twenty patients were selected. Microbiological samples were taken after accessing the canal, endodontic therapy, and PDT. At the end of the first session, the root canal was filled with Ca(OH)(2), and after 1 week, a second session of the therapies was performed. Endodontic therapy gave a mean reduction of 1.08 log. The combination with PDT significantly enhanced the reduction (1.83 log, p = 0.00002). The second endodontic session gave a similar diminution to the first (1.14 log), and the second PDT was significantly more effective than the first (p = 0.002). The second total reduction was significantly higher than the second endodontic therapy (p = 0.0000005). The total first + second reduction (3.19 log) was significantly different from the first combination (p = 0.00006). Results suggest that the use of PDT added to endodontic treatment leads to an enhanced decrease of bacterial load and may be an appropriate approach for the treatment of oral infections.
authors.affiliation: Centro de Laser e Aplicações, IPEN-CNEN/SP, São Paulo, Brazil.
authors.forename: Aguinaldo Silva
authors.initials: AS
authors.lastname: Garcez
authors.forename: Silvia Cristina
authors.initials: SC
authors.lastname: Nuñez
authors.forename: Michael R
authors.initials: MR
authors.lastname: Hamblin
authors.forename: Martha Simões
authors.initials: MS
authors.lastname: Ribeiro
mesh_terms: D000328:Adult; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D002126:Calcium Hydroxide / Q000627:therapeutic use; D015169:Colony Count, Microbial; D003131:Combined Modality Therapy; D003481:Cuspid / Q000382:microbiology; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000188:drug therapy* / Q000382:microbiology / Q000628:therapy; D006801:Humans; D007097:Imines / Q000627:therapeutic use; D007180:Incisor / Q000382:microbiology; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D010485:Periapical Periodontitis / Q000188:drug therapy* / Q000382:microbiology / Q000628:therapy; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D011095:Polyethylenes / Q000627:therapeutic use; D011107:Polylysine / Q000031:analogs & derivatives / Q000627:therapeutic use; D011166:Porphyrins / Q000627:therapeutic use; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D018915:Root Canal Preparation / Q000379:methods; D012390:Root Canal Therapy
chemical_list: D007097:Imines; D017319:Photosensitizing Agents; D011095:Polyethylenes; D011166:Porphyrins; D012387:Root Canal Filling Materials; C419690:clorin e6-(Lys)5-OH; C505405:poly(ethylene imine); D011107:Polylysine; D002126:Calcium Hydroxide