Extended depth of focus in tomographic phase microscopy using a propagation algorithm.
Tomographic phase microscopy is a laser interferometry technique in which a 3D refractive index map of a biological sample is constructed from quantitative phase images collected at a set of illumination angles. Although the resulting tomographic images provide valuable information, their resolution declines at axial distances beyond about 1 microm from the focal plane. We describe an improved 3D reconstruction algorithm in which the field at the focal plane is numerically propagated to depths throughout the sample. Diffraction is thus incorporated, extending the depth of focus to more than 10 mum. Tomograms with improved focal depth are demonstrated for single HT29 cells.
Choi
Wonshik
W
GR Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. wonshik@mit.edu
Fang-Yen
Christopher
C
Badizadegan
Kamran
K
Dasari
Ramachandra R
RR
Feld
Michael S
MS
D045744:Cell Line, Tumor; D006801:Humans; D007091:Image Processing, Computer-Assisted; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*