[Effects of intracoronary transplantation of autologous bone marrow mononuclear cells or endothelial progenitor cells in mini-swine model of myocardial ischemia-reperfusion].
OBJECTIVE
To compare the effects of intracoronary transplantation of autologous bone marrow mononuclear cells (BM-MNC) or peripheral endothelial progenitor cells (EPC) in mini-swine model of myocardial ischemia-reperfusion.


METHODS
The Mini-swine acute myocardial infarction and reperfusion model was created with 90 min occlusion of the left anterior descending coronary artery followed by reperfusion and the animals were then divided into BM-MNC group (3.54 x 10(8) +/- 0.90 x 10(8), n = 9), EPC group (1.16 x 10(7) +/- 1.07 x 10(7), n = 7) and control group (saline, n = 7). Echocardiography, hemodynamic measurements and myocardium infarction size were evaluated before and 4 weeks after intracoronary cell transplantations.


RESULTS
The net decrease from baseline to 4 weeks after transplantation of left ventricular ejection fraction (LVEF), left ventricular end systolic pressure, cardiac output and +dp/dt(max) were significantly attenuated post BM-MNC and EPC therapy compared to control group (all P < 0.05) and were similar between BM-MNC and EPC groups. Transplantation of BM-MNC and EPC also significantly decreased myocardial infarction size compared to control group.


CONCLUSIONS
Autologous intracoronary transplantation of BM-MNC or EPC in this model equally improved cardiac systolic function and reduced infarction area.
Li
Chong-jian
CJ
Coronary Heart Disease Center, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100037, China.
Gao
Run-lin
RL
Yang
Yue-jin
YJ
Hu
Feng-huan
FH
Yang
Wei-xian
WX
Song
Lai-feng
LF
Ruan
Ying-mao
YM
Qiao
Shu-bin
SB
Chen
Ji-lin
JL
Qin
Xue-wen
XW
Chen
Zai-jia
ZJ
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation; D003326:Coronary Circulation; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology; D005260:Female; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000628:therapy*; D013234:Stem Cells / Q000166:cytology; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous