Recombinant egg drop syndrome subunit vaccine offers an alternative to virus propagation in duck eggs.
Egg drop syndrome (EDS) virus vaccines are routinely produced in embryonated duck eggs (Solyom et al., 1982). This procedure poses the risk of dissemination of pathogens, such as avian influenza virus, as the eggs used are not from specific pathogen free birds. To address this problem, the knob and part of the shaft domain of the fibre protein of the EDS virus (termed knob-s) were expressed in Escherichia coli and assessed as a subunit vaccine. A single vaccination with the recombinant protein induced the production of anti-EDS virus antibodies, as detected by haemagglutination inhibition, enzyme-linked immunosorbent assay and virus neutralization tests, for at least 20 weeks. A positive correlation was demonstrated between these three assays. A dose-response assessment showed that the vaccine was effective over the range of 2 to 64 microg protein per dose. Two vaccinations with the recombinant protein, administered before the onset of lay, induced high haemagglutination inhibition antibody titres, comparable with those induced by an inactivated whole-virus vaccine. The vaccine did not have any adverse effects on egg production, quality or weight. The present study has shown that two vaccinations with the recombinant knob-s protein elicited high neutralizing antibody titres that persisted for more than 50 weeks of lay.
Gutter
B
B
ABIC, West Industrial Zone, Beit Shemesh, Israel.
Fingerut
E
E
Gallili
G
G
Eliahu
D
D
Perelman
B
B
Finger
A
A
Pitcovski
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D046808:Atadenovirus / Q000276:immunology*; D002645:Chickens; D003001:Cloning, Molecular; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D010063:Ovum / Q000821:virology*; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000276:immunology*; D014776:Virus Cultivation / Q000379:methods / Q000662:veterinary*
D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines