title: Isolation of primary human hepatocytes after partial hepatectomy: criteria for identification of the most promising liver specimen.
abstract: Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two-step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma-glutamyl-transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties.
authors.affiliation: Department of General, Visceral, and Transplantation Surgery, Charité Universitätsmedizin Berlin, Campus Virchow, Berlin, Germany. florian@vondran.org
authors.forename: Florian Wolfgang Rudolf
authors.initials: FW
authors.lastname: Vondran
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Katenz
authors.forename: Ruth
authors.initials: R
authors.lastname: Schwartlander
authors.forename: Mehmet Haluk
authors.initials: MH
authors.lastname: Morgul
authors.forename: Nathanael
authors.initials: N
authors.lastname: Raschzok
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Gong
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Cheng
authors.forename: Daniel
authors.initials: D
authors.lastname: Kehr
authors.forename: Igor Maximilian
authors.initials: IM
authors.lastname: Sauer
mesh_terms: D000328:Adult; D000368:Aged; D000418:Albumins / Q000096:biosynthesis; D001219:Aspartate Aminotransferases / Q000378:metabolism; D018929:Cell Culture Techniques; D002469:Cell Separation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D006498:Hepatectomy; D022781:Hepatocytes / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D014176:Protein Biosynthesis; D013997:Time Factors; D014508:Urea / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000418:Albumins; D014508:Urea; D007770:L-Lactate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases