title: Effects of FGF-2 release from a hydrogel polymer on bone mass and microarchitecture.
abstract: Bone substitutes are widely used for filling and restoring bone defects. Among them, methacrylic polymers are employed in load-bearing bones to seal hip prostheses. Incorporation of growth factors into a polymer device could be a way to enhance bone growth. In the present study, we evaluated the capacity of poly(2-hydroxyethyl methacrylate) - pHEMA - copolymerized with 2-vinyl pyrrolidone - VP - to release proteins. Fibroblast growth factor-2 (FGF-2) was incorporated into cylinders of p(HEMA-co-VP). FGF-2 release was studied by ELISA in vitro and cylinders were implanted in the femoral condyle of white New Zealand rabbits. After 2 months post-surgery, FGF-2 was able to enhance bone formation by increasing bone volume; this effect was evidenced by an increase in trabecular number and bone gain was mainly in the form of woven bone. At 3 months post-surgery, no difference could be evidenced between animals receiving vehicle or FGF-2. Animals receiving vehicle exhibited bone mass higher than at 2 months and woven bone was replaced by mature bone with a lamellar matrix. The hydrogel polymer allowed the release of FGF-2, which in return enhanced bone regeneration soon after surgery but the effect vanished rapidly.
authors.affiliation: INSERM, U922 - LHEA, Faculté de Médecine, 49045 Angers Cedex, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mabilleau
authors.forename: Eric
authors.initials: E
authors.lastname: Aguado
authors.forename: Izabella C
authors.initials: IC
authors.lastname: Stancu
authors.forename: Corneliu
authors.initials: C
authors.lastname: Cincu
authors.forename: Michel F
authors.initials: MF
authors.lastname: Baslé
authors.forename: Daniel
authors.initials: D
authors.lastname: Chappard
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000166:cytology* / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000601:surgery; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019736:Prostheses and Implants; D011817:Rabbits; D014057:Tomography, X-Ray Computed
chemical_list: D011108:Polymers; D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate