Nucleoplasmic reticulum is not essential in nuclear calcium signalling mediated by cyclic ADPribose in primary neurons.
Nuclear calcium regulation is essential for controlling nuclear processes such as gene expression. Recent studies, mostly performed on immortalized or transformed cell lines, reported the presence of a nucleoplasmic reticulum (NR). It has been suggested that NR acts as a storage organelle having an important role in nuclear Ca2+ signalling. However, whether NR is present and necessary in primary neurons for generation of nuclear Ca2+ signalling has never been investigated. Here, we show, by confocal microscopy and by electronic microscopy, that nuclei in intact neurons or isolated nuclei are not endowed with NR. Finally, our experiments performed on isolated nuclei from Aplysia giant neurons show that the nuclear envelope acts as a functional Ca2+ store which can be mobilized by the second messenger cyclic ADPribose to elicit a nucleoplasmic Ca2+ elevation. Our study provides evidence that nuclear Ca2+ signals can be independent of the presence of NR in neurons.
Bezin
S
S
CNRS, Institut de Neurobiologie Alfred Fessard-FRC 2118, Laboratoire de Neurobiologie Cellulaire et Moléculaire-UPR9040, 91198 Gif sur Yvette, France.
Fossier
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Cancela
J-M
JM
D000818:Animals; D001048:Aplysia; D020013:Calcium Signaling; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D036563:Cyclic ADP-Ribose / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017952:Ganglia, Invertebrate / Q000378:metabolism* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000378:metabolism
D036563:Cyclic ADP-Ribose