title: Identification of biological microparticles using ultrafast depletion spectroscopy.
abstract: We show how an ultrafast pump-pump excitation induces strong fluorescence depletion in biological samples, such as bacteria-containing droplets, in contrast with fluorescent interferents, such as polycyclic aromatic compounds, despite similar spectroscopic properties. Application to the optical remote discrimination of biotic versus non-biotic particles is proposed. Further improvement is required to allow the discrimination of one pathogenic among other non-pathogenic micro-organisms. This improved selectivity may be reached with optimal coherent control experiments, as discussed in the paper.
authors.affiliation: GAP-Biophotonics, University of Geneva, 20, rue de l'Ecole de Médecine, 1211, Geneva 4, Switzerland.
authors.forename: Francois
authors.initials: F
authors.lastname: Courvoisier
authors.forename: Luigi
authors.initials: L
authors.lastname: Bonacina
authors.forename: Véronique
authors.initials: V
authors.lastname: Boutou
authors.forename: Laurent
authors.initials: L
authors.lastname: Guyon
authors.forename: Christophe
authors.initials: C
authors.lastname: Bonnet
authors.forename: Benoit
authors.initials: B
authors.lastname: Thuillier
authors.forename: Jerome
authors.initials: J
authors.lastname: Extermann
authors.forename: Matthias
authors.initials: M
authors.lastname: Roth
authors.forename: Herschel
authors.initials: H
authors.lastname: Rabitz
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Wolf
mesh_terms: D000336:Aerosols / Q000737:chemistry; D001419:Bacteria / Q000302:isolation & purification; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000336:Aerosols