title: Expression of GABArho subunits during rat cerebellum development.
abstract: In the present study, we provide evidence for the expression of all three GABA(C) receptor rho subunits through development of the rat cerebellum. Injection of cerebellum mRNA into frog oocytes gave rise to the expression of both GABA(A) and GABA(C) receptors. qRT-PCR of RNA isolated from postnatal developing cerebella showed that the expression of each rho subunit is relatively low, with a relative comparative expression of rho3>rho1>rho2. In situ hybridization and immunohistochemistry revealed a limited distribution of GABA(C) receptors in the Purkinje and Golgi neurons whereas electron microscopy detected the rho1 and rho2 subunits in the soma and dendritic tree of the Purkinje cells. The expression of GABA(C) receptors in the cerebellum adds a new dimension to the regulation of GABAergic neurotransmission and suggests further experiments to determine their functional consequences.
authors.affiliation: Instituto de Neurobiología, Departamento de Neurobiología Molecular y Celular, Universidad Nacional Autónoma de México-Campus Juriquilla, (Boulevar) Universitario 3001, Querétaro, 76230, Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Mejía
authors.forename: Guadalupe
authors.initials: G
authors.lastname: García-Alcocer
authors.forename: Laura C
authors.initials: LC
authors.lastname: Berumen
authors.forename: Abraham
authors.initials: A
authors.lastname: Rosas-Arellano
authors.forename: Ricardo
authors.initials: R
authors.lastname: Miledi
authors.forename: Ataúlfo
authors.initials: A
authors.lastname: Martínez-Torres
mesh_terms: D000818:Animals; D001001:Anura; D002531:Cerebellum / Q000254:growth & development* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D008297:Male; D016253:Microscopy, Immunoelectron; D009865:Oocytes / Q000502:physiology; D011689:Purkinje Cells / Q000502:physiology / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000235:genetics* / Q000378:metabolism; D011963:Receptors, GABA-A; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C528239:Gabrr1 protein, rat; C491397:Gabrr2 protein, rat; C528240:Gabrr3 protein, rat; D012333:RNA, Messenger; D018079:Receptors, GABA; D011963:Receptors, GABA-A