Cell response to magnetic glyconanoparticles: does the carbohydrate matter?
Magnetic nanoparticles have been used in therapeutic and diagnostic approaches in biomedicine for many years. For these applications, it is very important to investigate the nanoparticle-cell interactions. In this study we report a simple method for the preparation of gold-iron nanoparticles protected and functionalized with biologically relevant saccharides (maltose, lactose, and glucose). The nanoparticles were subsequently tested in vitro with a human fibroblast cell line to determine biocompatibility, and the cell-particle interactions, using fluorescence and scanning electron microscopies. Different cellular responses were obtained for each type of glyconanoparticle, demonstrating that the cells can recognize the saccharides on the nanoparticles.
de la Fuente
Jesús M
JM
Laboratory of Glyconanotechnology, IIQ-CSIC, 41092 Seville, Spain. jmfuente@unizar.es
Alcántara
David
D
Penadés
Soledad
S
D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003603:Cytotoxins / Q000378:metabolism; D004705:Endocytosis; D005290:Ferric Compounds / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D008280:Magnetics; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000008:administration & dosage / Q000633:toxicity
D002241:Carbohydrates; D003603:Cytotoxins; D005290:Ferric Compounds; D006046:Gold