IL-10 overexpression decreases inflammatory mediators and promotes regenerative healing in an adult model of scar formation.
Adult wound healing is characterized by an exuberant inflammatory response and scar formation. In contrast, scarless fetal wound healing has diminished inflammation, a lack of fibroplasia, and restoration of normal architecture. We have previously shown that fetal wounds produce less inflammatory cytokines, and the absence of IL-10, an anti-inflammatory cytokine, results in fetal scar formation. We hypothesized that increased IL-10 would decrease inflammation and create an environment conducive for regenerative healing in the adult. To test this hypothesis, a lentiviral vector expressing IL-10 and green fluorescent protein (GFP) (Lenti-IL-10) or GFP alone (Lenti-GFP) was injected at the wound site 48 hours before wounding. We found that both Lenti-IL-10 and Lenti-GFP were expressed in the wounds at 1 and 3 days post wounding. At 3 days, Lenti-IL-10-treated wounds demonstrated decreased inflammation and decreased quantities of all proinflammatory mediators analyzed with statistically different levels of IL-6, monocyte chemoattractant protein-1, and heat-shock protein 47. At 3 weeks, Lenti-GFP wounds demonstrated scar formation. In contrast, wounds injected with Lenti-IL-10 demonstrated decreased inflammation, a lack of abnormal collagen deposition, and restoration of normal dermal architecture. We conclude that lentivirus-mediated overexpression of IL-10 decreases the inflammatory response to injury, creating an environment conducive for regenerative adult wound healing.
Peranteau
William H
WH
Department of Surgery, The Center for Fetal Research, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104-4318, USA.
Zhang
Liping
L
Muvarak
Nidal
N
Badillo
Andrea T
AT
Radu
Antoneta
A
Zoltick
Philip W
PW
Liechty
Kenneth W
KW
D000818:Animals; D002921:Cicatrix / Q000503:physiopathology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D050958:HSP47 Heat-Shock Proteins / Q000502:physiology; D007249:Inflammation / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism*; D016753:Interleukin-10 / Q000502:physiology*; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D012038:Regeneration; D014945:Wound Healing / Q000502:physiology*
D050958:HSP47 Heat-Shock Proteins; D018836:Inflammation Mediators; D016753:Interleukin-10; D049452:Green Fluorescent Proteins