Critical role of a single position in the -35 element for promoter recognition by Mycobacterium tuberculosis SigE and SigH.
Mycobacterial SigE and SigH both initiate transcription from the sigB promoter, suggesting that they recognize similar sequences. Through mutational and primer extension analyses, we determined that SigE and SigH recognize nearly identical promoters, with differences at the 3' end of the -35 element distinguishing between SigE- and SigH-dependent promoters.
Song
Taeksun
T
The Genome Research Center for Respiratory Pathogens, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
Song
Seung-Eun
SE
Raman
Sahadevan
S
Anaya
Mauricio
M
Husson
Robert N
RN
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C120328:SigH protein, bacteria; D012808:Sigma Factor; C111259:sigE protein, Bacteria