title: Genome biology of Actinobacillus pleuropneumoniae JL03, an isolate of serotype 3 prevalent in China.
abstract: Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, a cause of considerable world wide economic losses in the swine industry. We sequenced the complete genome of A. pleuropneumoniae, JL03, an isolate of serotype 3 prevalent in China. Its genome is a single chromosome of 2,242,062 base pairs containing 2,097 predicted protein-coding sequences, six ribosomal rRNA operons, and 63 tRNA genes. Preliminary analysis of the genomic sequence and the functions of the encoded proteins not only confirmed the present physiological and pathological knowledge but also offered new insights into the metabolic and virulence characteristics of this important pathogen. We identified a full spectrum of genes related to its characteristic chemoheterotrophic catabolism of fermentation and respiration with an incomplete TCA system for anabolism. In addition to confirming the lack of ApxI toxin, identification of a nonsense mutation in apxIVA and a 5'-proximal truncation of the flp operon deleting both its promoter and the flp1flp2tadV genes have provided convincing scenarios for the low virulence property of JL03. Comparative genomic analysis using the available sequences of other serotypes, probable strain (serotype)-specific genomic islands related to capsular polysaccharides and lipopolysaccharide O-antigen biosyntheses were identified in JL03, which provides a foundation for future research into the mechanisms of serotypic diversity of A. pleuropneumoniae.
authors.affiliation: Division of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Zhuofei
authors.initials: Z
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Liangjun
authors.initials: L
authors.lastname: Li
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
authors.forename: Yun
authors.initials: Y
authors.lastname: Wan
authors.forename: Sihua
authors.initials: S
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.forename: Mingsong
authors.initials: M
authors.lastname: Kang
authors.forename: Baolige
authors.initials: B
authors.lastname: Dalai
authors.forename: Tingting
authors.initials: T
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Yangyi
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Huajun
authors.initials: H
authors.lastname: Zheng
authors.forename: Shiying
authors.initials: S
authors.lastname: Pu
authors.forename: Bofei
authors.initials: B
authors.lastname: Wang
authors.forename: Wenyi
authors.initials: W
authors.lastname: Gu
authors.forename: Xiang-Lin
authors.initials: XL
authors.lastname: Zhang
authors.forename: Geng-Feng
authors.initials: GF
authors.lastname: Zhu
authors.forename: Shengyue
authors.initials: S
authors.lastname: Wang
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D001422:Bacterial Adhesion; D002681:China; D016680:Genome, Bacterial; D014774:Virulence