Delayed rectifier potassium channels are involved in SO2 derivative-induced hippocampal neuronal injury.
Recent studies implicate the possible neurotoxicity of SO(2), however, its mechanisms remain unclear. In the present study, we investigated SO(2) derivative-induced effect on delayed rectifier potassium channels (I(K)) and cellular death/apoptosis in primary cultured hippocampal neurons. The results demonstrate that SO(2) derivatives (NaHSO(3) and Na(2)SO(3), 3:1M/M) effectively augmented I(K) and promoted the activation of delayed rectifier potassium channels. Also, SO(2) derivatives increased neuronal death percentage and contributed to the formation of DNA ladder in concentration-dependent manners. Interestingly, the neuronal death and DNA ladder formation, caused by SO(2) derivatives, could be attenuated by the delayed rectifier potassium channel blocker (tetraethylammonium, TEA), but not by the transient outward potassium channel blocker (4-aminopyridine, 4-AP). It implies that stimulating delayed rectifier potassium channels were involved in SO(2) derivative-caused hippocampal neuronal insults, and blocking these channels might be one of the possibly clinical treatment for SO(2)-caused neuronal dysfunction.
Li
Guangke
G
College of Environment and Resource, Center of Environment Science and Engineering, Shanxi University, Taiyuan, Shanxi 030006, PR China.
Sang
Nan
N
College of Environment and Resource, Center of Environment Science and Engineering, Shanxi University, Taiyuan, Shanxi 030006, PR China. Electronic address: sangnan_lgkcarl@yahoo.com.cn.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D051668:Delayed Rectifier Potassium Channels / Q000187:drug effects / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology*; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D009498:Neurotoxins / Q000633:toxicity*; D018408:Patch-Clamp Techniques / Q000379:methods; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000494:pharmacology; D013458:Sulfur Dioxide / Q000633:toxicity*; D014947:Wounds and Injuries / Q000188:drug therapy
D051668:Delayed Rectifier Potassium Channels; D009498:Neurotoxins; D026902:Potassium Channel Blockers; D013431:Sulfates; D013458:Sulfur Dioxide; C012036:sodium sulfate