title: [Folic acid attenuates homocysteine induced human monocytes chemokine secretion via reducing NADPH oxidase activity].
abstract: OBJECTIVE
  To investigate the effect of folic acid on homocysteine (Hcy)-induced chemokine secretion and NADPH oxidase activity in human monocytes.
  
  
  METHODS
  Human monocytes from healthy volunteers were incubated with Hcy (100 micromol/L) with or without folic acid (5 micromol/L) for 24 h; MCP-1 and IL-8 were assessed by ELISA. DCFH-DA was added to monitor intracellular ROS production on confocal microscopy. A cytochrome c reduction assay was used to measure NADPH oxidase activity.
  
  
  RESULTS
  The Hcy-induced secretion of MCP-1 and IL-8 was significantly reduced by folic acid [(1.88 +/- 0.51) ng/ml vs. (4.36 +/- 0.72) ng/ml vs. (2.40 +/- 0.60) ng/ml and (4.9 +/- 1.9) ng/ml vs. (12.7 +/- 1.5) ng/ml vs. (7.2 +/- 1.9) ng/ml, all P < 0.05]. The Hcy-induced production of ROS was also significantly attenuated by folic acid. Moreover, the Hcy-induced NADPH oxidase activity increase was significantly inhibited by cotreatment with folic acid.
  
  
  CONCLUSIONS
  Folic acid may attenuate oxidative stress induced by Hcy by reducing NADPH oxidase activity in monocytes.
authors.affiliation: Cardiovascular Medicine, Beijing Haidian Hospital, Beijing 100080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Guang
authors.initials: G
authors.lastname: Wang
authors.forename: Fu-chun
authors.initials: FC
authors.lastname: Zhang
authors.forename: Jie-ming
authors.initials: JM
authors.lastname: Mao
authors.forename: Jing
authors.initials: J
authors.lastname: Dai
mesh_terms: D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism*; D005492:Folic Acid / Q000494:pharmacology*; D006710:Homocysteine / Q000494:pharmacology*; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D054390:Receptors, CCR2 / Q000378:metabolism
chemical_list: D018925:Chemokines; D016209:Interleukin-8; D054390:Receptors, CCR2; D006710:Homocysteine; D005492:Folic Acid; D019255:NADPH Oxidases