Heat shock protein 27 protects against aminolevulinic acid-mediated photodynamic therapy-induced apoptosis and necrosis in human breast cancer cells.
This study utilized two breast cancer cell lines differing only in their expression of heat shock protein 27 (hsp27). The DB46 cell line was engineered to express high constitutive levels of hsp27, while the DC4 cell line expresses normal low levels of hsp27. The cells were incubated in 1 mM aminolevlinic acid (ALA) 4 hr prior to light exposures (635 nm) ranging from 1 to 20 J/cm2. Both cell lines displayed a dose response to photodynamic therapy (PDT) as assayed by clonogenic survival. LD50s of 2.68 and 1.27 J/cm2 were observed for DB46 and DC4 cells respectively. ALA-PDT-induced resistance to both apoptosis and necrosis in the DB46 cell line was found from TUNEL assays and fluorescence microscopy studies using propidium iodide and Hoechst staining.
Ziegler
Sarah A
SA
Department. of Chemistry, University of Nevada Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154-4003, USA.
Loucks
Cherisse
C
Madsen
Steen J
SJ
Carper
Stephen W
SW
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000528:radiation effects; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006360:Heat-Shock Proteins / Q000494:pharmacology*; D006801:Humans; D009336:Necrosis; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid