Detection of gamma-tubulin in spermatogonial cells of Bombyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera).
We detected a putative gamma-tubulin gene in silico and detected BACs containing the gene from a Bombyx mori BAC library. BAC-FISH mapping revealed that the gene is located on chromosome 5. To observe the distribution of gamma-tubulin, we employed antibodies against mammalian gamma-tubulin peptides. Western blot analysis disclosed a band very similar in size to gamma-tubulin protein in other species (approximately 48 kDa). In mitotic metaphase of B. mori spermatogonial cells, gamma-tubulin is exclusively localized in the spindle poles, where the centrosomes occur. We applied the same system to the grasshopper Chortophaga viridifasciata, as a representative of insect orders in which the gamma-tubulin distribution had not previously been studied. Gamma-tubulin was also found in the spindle poles during metaphase of spermatogonial cells in the grasshopper.
Matsuda
Yumi
Y
Laboratory of Developmental Biology, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
Sahara
Ken
K
Yasukochi
Yuji
Y
Yamashiki
Naoko
N
D000818:Animals; D000941:Antigens / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D012831:Bombyx; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D008297:Male; D008938:Mitosis / Q000276:immunology; D009987:Orthoptera; D013045:Species Specificity; D013094:Spermatozoa / Q000502:physiology*; D008941:Spindle Apparatus / Q000737:chemistry*; D014404:Tubulin / Q000235:genetics / Q000276:immunology*
D000941:Antigens; D014404:Tubulin