Polyamines are essential in embryo implantation: expression and function of polyamine-related genes in mouse uterus during peri-implantation period.
Polyamines are key regulators in cell growth and differentiation. It has been shown that ornithine decarboxylase (Odc) was essential for post-implantation embryo development, and overexpression of spermidine/spermine N1-acetyltransferase will lead to ovarian hypofunction and hypoplastic uteri. However, the expression and function of polyamine-related genes in mouse uterus during early pregnancy are still unknown. In this study we investigated the expression, regulation, and function of polyamine-related genes in mouse uterus during the peri-implantation period. Odc expression was strongly detected at implantation sites and stimulated by estrogen treatment. The expression of Odc antizyme 1 and spermidine/spermine N1-acetyltransferase was also highly shown at implantation sites and regulated by Odc or polyamine level in uterine cells. Embryo implantation was significantly inhibited by alpha-difluoromethylornithine, an Odc inhibitor. Moreover, the reduction of Odc activity caused by alpha-difluoromethylornithine treatment was compensated by the up-regulation of S-adenosylmethionine decarboxylase gene expression. Collectively, our results indicated that the coordinated expression of uterine polyamine-related genes may be important for embryo implantation.
Zhao
Yue-Chao
YC
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, College of Life Science, Xiamen University, Xiamen 361005, China.
Chi
Yu-Jing
YJ
Yu
Yong-Sheng
YS
Liu
Ji-Long
JL
Su
Ren-Wei
RW
Ma
Xing-Hong
XH
Shan
Chun-Hua
CH
Yang
Zeng-Ming
ZM
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics; D000818:Animals; D000518:Eflornithine / Q000494:pharmacology; D010064:Embryo Implantation / Q000187:drug effects* / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D008297:Male; D051379:Mice; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D010052:Ovariectomy; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism / Q000494:pharmacology*; D011247:Pregnancy; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011555:Pseudopregnancy / Q000235:genetics; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism; D000097667:Polyamine Oxidase
D012437:Adenosylmethionine Decarboxylase; D000518:Eflornithine; D012739:Gonadal Steroid Hormones; D009955:Ornithine Decarboxylase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D011506:Proteins; D000097667:Polyamine Oxidase; C031017:ornithine decarboxylase antizyme