Antifungal activity of essential oil and its constituents from Calocedrus macrolepis var. formosana Florin leaf against plant pathogenic fungi.
Resistance to conventional fungicides causes the poor disease control of agriculture. Natural products from plants have great potential as novel fungicide sources for controlling pathogenic fungi. In this study antipathogenic activity of the leaf essential oil and its constituents from Calocedrus macrolepis var. formosana Florin were evaluated in vitro against six plant pathogenic fungi. Chemical analysis of leaf oil by GC/MS allowed identification of alpha-pinene (44.2%), limonene (21.6%), beta-myrcene (8.9%), beta-caryophyllene (8.2%), caryophyllene oxide (2.4%), alpha-cadinol (1.6%), beta-pinene (1.2%), and T-muurolol (1.1%) as main components. Sesquiterpenoid components of the oil were more effective than monoterpenoid components of the oil. In particular, T-muurolol and alpha-cadinol strongly inhibited the growth of Rhizoctonia solani and Fusarium oxysporum, with the IC(50) values < 50 microg ml(-1). These compounds also efficiently inhibited the mycelial growths of Colletotrichum gloeosporioides, P. funerea, Ganoderma australe and F. solani. These results showed that T-muurolol and alpha-cadinol possess antifungal activities against a broad spectrum of tested plant pathogenic fungi and could be used as potential antifungal agents for the control of fungal diseases in plants.
Chang
Hui-Ting
HT
School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan. chtchang@ntu.edu.tw
Cheng
Ying-Hung
YH
Wu
Chi-Lin
CL
Chang
Shang-Tzen
ST
Chang
Tun-Tschu
TT
Su
Yu-Chang
YC
D000935:Antifungal Agents / Q000494:pharmacology*; D029779:Cupressaceae / Q000737:chemistry* / Q000382:microbiology; D005658:Fungi / Q000472:pathogenicity; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000737:chemistry* / Q000382:microbiology
D000935:Antifungal Agents; D009822:Oils, Volatile