title: A mouse model of intracerebral hemorrhage using autologous blood infusion.
abstract: The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.
authors.affiliation: Department of Neurological Surgery, Columbia University, 710 West 168th Street, New York, New York 10032, USA.
authors.forename: Michal A
authors.initials: MA
authors.lastname: Rynkowski
authors.forename: Grace H
authors.initials: GH
authors.lastname: Kim
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Komotar
authors.forename: Marc L
authors.initials: ML
authors.lastname: Otten
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Ducruet
authors.forename: Brad E
authors.initials: BE
authors.lastname: Zacharia
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Kellner
authors.forename: David K
authors.initials: DK
authors.lastname: Hahn
authors.forename: Maxwell B
authors.initials: MB
authors.lastname: Merkow
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Garrett
authors.forename: Robert M
authors.initials: RM
authors.lastname: Starke
authors.forename: Byung-Moon
authors.initials: BM
authors.lastname: Cho
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Sosunov
authors.forename: E Sander
authors.initials: ES
authors.lastname: Connolly
mesh_terms: D000818:Animals; D001479:Basal Ganglia; D001804:Blood Transfusion, Autologous; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000209:etiology*; D004195:Disease Models, Animal; D007260:Infusion Pumps; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011182:Postoperative Care