Partial characterization and expression of leaf catalase in the CAM-inducible halophyte Mesembryanthemum crystallinum L.
Catalase (CAT; EC 1.11.1.6) isolated from leaves of the halophytic plant Mesembryanthemum crystallinum is characterized by a high apparent molecular mass of about 320kDa, and high resistance to denaturing agents (10% ME). SDS-treatment breaks active oligomeric CAT into the less active and putatively dimeric form of 160kDa apparent molecular mass. Three subunits are resolved after denaturing PAGE: 79, 74 and 62kDa. Higher molecular masses of subunits coincide with increased activity of CAT. M. crystallinum leaf CAT reveals a diel variation in the resistance to denaturing factors and the stability of CAT is increased in a light-dependent manner both in C(3)- and in CAM-induced plants. Unchanged level of leaf CAT transcripts is documented in the diurnal cycle of C(3) plants and after salinity-induced crassulacean acid metabolism (CAM).
Niewiadomska
Ewa
E
Institute of Plant Physiology, Polish Academy of Sciences, Krakow, Poland. e.niewiadomska@ifr-pan.krakow.pl
Miszalski
Zbigniew
Z
D002374:Catalase / Q000737:chemistry*; D004789:Enzyme Activation / Q000502:physiology; D004795:Enzyme Stability / Q000502:physiology; D035723:Mesembryanthemum / Q000201:enzymology*; D018515:Plant Leaves / Q000201:enzymology*; D021122:Protein Subunits / Q000737:chemistry; D054712:Salinity
D021122:Protein Subunits; D002374:Catalase