[Amount of circulating endothelium precursors in patients with stable angina of effort and patients with acute non-ST-elevation coronary syndrome].
OBJECTIVE
To measure and compare the level of circulating precursors of endothelial cells in patients with acute coronary non-ST-elevation syndrome (NSTES), patients with stable effort angina (SEA) before coronary stenting and after it and persons with documented absence of coronary heart disease (CHD).


METHODS
The trial included 19 patients with acute coronary NSTES, 20 patients with SEA and 14 patients free of CHD. Blood samples were stained with antibodies CD45-FITC (Becton Dickinson), CD34-PerCP (Becton Dickinson), KDR-PE (R&D systems). Count of cell populations was measured with flow cytofluorimetry (FACSCalibur (Becton Dickinson).


RESULTS
SEA patients had the number of circulating cells CD34+ including hematopoietic cells and endothelial precursors (EP) two times lower versus that in the controls (0.650 +/- 0.086 and 1.216 +/- 0.242%, respectively, p = 0.037). No significant differences were found by the number of CD34+KDR+ cells between the groups. A trend was found to lower number of CD34-KDR+ cells in EA patients compared to control. The differences reached significance on day 1 and 3-5 after endovascular interventions (0.067 +/- 0.022, 0.060 +/- 0.017 and 0.188 +/- 0.052%, respectively, p = 0.024 and p = 0.021).


CONCLUSIONS
The count of blood CD34+ cells in combination with other indicators can be used as an additional factor confirming the presence of chronic myocardial ischemia.
Vyborov
O N
ON
Aref'eva
T I
TI
Kalinina
N I
NI
Parfenova
E V
EV
D054058:Acute Coronary Syndrome / Q000097:blood / Q000378:metabolism*; D000787:Angina Pectoris / Q000097:blood / Q000378:metabolism*; D018952:Antigens, CD34 / Q000097:blood; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood; D005082:Physical Exertion; D012849:Sinoatrial Node / Q000502:physiology*
D018952:Antigens, CD34; D017493:Leukocyte Common Antigens