title: An in vitro evaluation of the growth of human periodontal ligament fibroblasts after exposure to a methacrylate-based endodontic sealer.
abstract: The cytotoxicity of Epiphany root canal sealer at various concentrations from 25-800 microg/mL on human periodontal ligament (HPDL) fibroblasts was evaluated at 1, 3, and 7 days. Controls included untreated cells and cells treated with the vehicle for Epiphany suspension. Fibroblast viability was assessed by 2 methods, crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates. Significant cytotoxicity against HPDL fibroblast growth by Epiphany was both time- and concentration-dependent. On day 1, 800 microg/mL, the highest concentration of Epiphany, showed significant cytotoxicity (P < or = .001). By day 7, all concentrations greater than 25 microg/mL showed significant (P < or = .05) loss of viability. This study demonstrated increased Epiphany cytotoxicity with an increase in concentration or exposure time.
authors.affiliation: U.S. Army Endodontic Residency Program, Fort Gordon, Georgia 30905, USA. erich.heitman@us.army.mil
authors.forename: Erich P
authors.initials: EP
authors.lastname: Heitman
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Joyce
authors.forename: James C
authors.initials: JC
authors.lastname: McPherson
authors.forename: Steven
authors.initials: S
authors.lastname: Roberts
authors.forename: Augustine
authors.initials: A
authors.lastname: Chuang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D005840:Gentian Violet; D006801:Humans; D008689:Methacrylates / Q000633:toxicity*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000633:toxicity*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D008689:Methacrylates; D012387:Root Canal Filling Materials; C486893:epiphany sealer; D005840:Gentian Violet