title: Interferon-gamma upregulates retinoic acid-inducible gene-I in human pericardial mesothelial cells.
abstract: OBJECTIVE
  Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family proteins and serves as an intracellular signalling molecule involved in the regulation of various genes. Mesothelial cells are active in generating a variety of bioactive factors, and RIG-I may serve as a potential intracellular molecule regulating part of these factors. We have addressed the expression of RIG-I in pericardial mesothelial cells.
  
  
  RESULTS
  Cultures of human mesothelial cells separated during cardiac surgery were established. Interferon (IFN)-gamma was found to stimulate the expression, in cultured mesothelial cells, of RIG-I mRNA and protein in time- and concentration-dependent manners. We also performed immunohistochemical analysis of heart tissues to examine the expression of RIG-I in mesothelial cells. Intense immunoreactivity was detected in reactive pericardial mesothelial cells in non-specific pericarditis and in dilated cardiomyopathy.
  
  
  CONCLUSIONS
  RIG-I may be involved in the inflammatory reaction in pericardial mesothelial cells.
authors.affiliation: Department of Surgery, Hirosaki University School of Medicine, Hirosaki, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Fumio
authors.initials: F
authors.lastname: Terasaki
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Mori
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tanji
authors.forename: Fuyuki
authors.initials: F
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kijima
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Suma
authors.forename: Koichi
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Ikuo
authors.initials: I
authors.lastname: Fukuda
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis* / Q000235:genetics; D004848:Epithelium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism* / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology*; D010496:Pericardium / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D018836:Inflammation Mediators; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases