Proteomic analysis of altered proteins in lymphoid organ of yellow head virus infected Penaeus monodon.
A comparative proteomic analysis was employed to identify altered proteins in the yellow head virus (YHV) infected lymphoid organ (LO) of Penaeus monodon. At 24 h post-infection, the infected shrimps showed obvious signs of infection, while the control shrimps remained healthy. Two-dimensional electrophoresis of proteins extracted from the LO revealed significant alterations in abundance of several proteins in the infected group. Protein identification by MALDI-TOF MS and nanoLC-ESI-MS/MS revealed significant increase of transglutaminase, protein disulfide isomerase, ATP synthase beta subunit, V-ATPase subunit A, and hemocyanin fragments. A significant decrease was also identified for Rab GDP-dissociation inhibitor, 6-phosphogluconate dehydrogenase, actin, fast tropomyosin isoform, and hemolymph clottable protein. Some of these altered proteins were further investigated at the mRNA level using real-time RT-PCR, which confirmed the proteomic data. Identification of these altered proteins in the YHV-infected shrimps may provide novel insights into the molecular responses of P. monodon to YHV infection.
Bourchookarn
Apichai
A
Institute of Molecular Biology and Genetics (IMBG), Mahidol University, Salaya Campus, Nakhonpathom, Thailand.
Havanapan
Phattara-Orn
PO
Thongboonkerd
Visith
V
Krittanai
Chartchai
C
D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008221:Lymphoid Tissue / Q000737:chemistry*; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000821:virology*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D012333:RNA, Messenger; D046828:Roniviridae
D011506:Proteins; D012333:RNA, Messenger