title: Superparamagnetic iron oxide nanoparticles (SPIONs): from synthesis to in vivo studies--a summary of the synthesis, characterization, in vitro, and in vivo investigations of SPIONs with particular focus on surface and colloidal properties.
abstract: In this work, we present a short summary of the synthesis and characterization of superparamagnetic iron oxide nanoparticles and their behavior in vitro and in vivo. Therefore, we have used various characterization techniques to deduce the physical particle size as well as magnetic properties. It is shown that the particle properties were significantly improved by a thermochemical treatment and dialysis, obtaining weakly interacting particles with a clear blocking temperature. We also present the interaction of polyvinyl alcohol and vinyl alcohol/vinyl amine copolymer-coated SPIONs with HELA cells. It is shown that the uptake increased significantly in the presence of a magnetic field and that surface functional groups had an impact on particle uptake and metabolic activity. Furthermore, the influences of the varied parameters (polymer type and therefore surface charge, cell medium, and serum) on the agglomeration rate and the cell uptake are presented and discussed. Finally, we briefly describe the intraarticular application of SPIONs in sheep, their uptake by synovial membrane, and their systemic distribution and elimination.
authors.affiliation: Laboratory of Powder Technology, Ecole Polytechnique Fédérale de Lausanne, Switzerland. alke.fink@epfl.ch
authors.forename: Alke
authors.initials: A
authors.lastname: Petri-Fink
authors.forename: Heinrich
authors.initials: H
authors.lastname: Hofmann
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000633:toxicity; D003102:Colloids / Q000737:chemistry*; D003470:Culture Media; D005290:Ferric Compounds / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000633:toxicity*; D036103:Nanotechnology; D010316:Particle Size; D044367:Phase Transition; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013499:Surface Properties; D044366:Transition Temperature; D014867:Water
chemical_list: D020099:Coated Materials, Biocompatible; D003102:Colloids; D003470:Culture Media; D005290:Ferric Compounds; D014867:Water; C000499:ferric oxide; D011142:Polyvinyl Alcohol; D052203:Ferrosoferric Oxide