Uses of microarray platforms in cancer: a correlative study between genomic copy number changes and their expression at mRNA and protein levels.
With the completion of the Human Genome Project, the microarray technology has evolved into a sophisticated platform by which complex diseases such as cancer, can be studied at the genome, transcriptome, and proteome levels. Here, various microarray platforms, namely comparative genomic hybridization, cDNA, oligonucleotide, and protein-based microarrays are exploited to study genomic copy-number changes in a human cancer cell line and correlate these genomic aberrations with their expression at mRNA and protein levels. The protocols described therein can be assimilated for the study of other human tissues including cancerous ones.
Al-Mulla
Fahd
F
Department of Pathology, Faculty of Medicine, Kuwait University, Safat.
Al-Tamimi
Raba
R
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D005260:Female; D018628:Gene Dosage; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm