title: Molecular probes: insights into design and analysis from computational and physical chemistry.
abstract: The application of new molecular diagnostics to probe cellular process in vivo is leading to a greater understanding of molecular cytology at a sub-nanoscale level and is opening the way to individualized medicines. We review here three distinct fluorescence-based molecular probes, HyBeacons, split-probe exciplexes and GFP (green fluorescent protein)-based FRET (fluorescence resonance energy transfer) systems. Through this, we highlight the insights into the mechanism and design that a combined computational and experimental approach can yield.
authors.affiliation: Wolfson Centre for Structure-Based Design of Molecular Diagnostics, School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PL, U.K.
authors.forename: Felicity L
authors.initials: FL
authors.lastname: Mitchell
authors.forename: Gabriel E
authors.initials: GE
authors.lastname: Marks
authors.forename: Elena V
authors.initials: EV
authors.lastname: Bichenkova
authors.forename: Kenneth T
authors.initials: KT
authors.lastname: Douglas
authors.forename: Richard A
authors.initials: RA
authors.lastname: Bryce
mesh_terms: D001483:Base Sequence; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D015335:Molecular Probes; D050505:Mutant Proteins; D009841:Oligonucleotides; D049452:Green Fluorescent Proteins