title: An integrated high-density linkage map of soybean with RFLP, SSR, STS, and AFLP markers using A single F2 population.
abstract: Soybean [Glycine max (L.) Merrill] is the most important leguminous crop in the world due to its high contents of high-quality protein and oil for human and animal consumption as well as for industrial uses. An accurate and saturated genetic linkage map of soybean is an essential tool for studies on modern soybean genomics. In order to update the linkage map of a F2 population derived from a cross between Misuzudaizu and Moshidou Gong 503 and to make it more informative and useful to the soybean genome research community, a total of 318 AFLP, 121 SSR, 108 RFLP, and 126 STS markers were newly developed and integrated into the framework of the previously described linkage map. The updated genetic map is composed of 509 RFLP, 318 SSR, 318 AFLP, 97 AFLP-derived STS, 29 BAC-end or EST-derived STS, 1 RAPD, and five morphological markers, covering a map distance of 3080 cM (Kosambi function) in 20 linkage groups (LGs). To our knowledge, this is presently the densest linkage map developed from a single F2 population in soybean. The average intermarker distance was reduced to 2.41 from 5.78 cM in the earlier version of the linkage map. Most SSR and RFLP markers were relatively evenly distributed among different LGs in contrast to the moderately clustered AFLP markers. The number of gaps of more than 25 cM was reduced to 6 from 19 in the earlier version of the linkage map. The coverage of the linkage map was extended since 17 markers were mapped beyond the distal ends of the previous linkage map. In particular, 17 markers were tagged in a 5.7 cM interval between CE47M5a and Satt100 on LG C2, where several important QTLs were clustered. This newly updated soybean linkage map will enable to streamline positional cloning of agronomically important trait locus genes, and promote the development of physical maps, genome sequencing, and other genomic research activities.
authors.affiliation: Faculty of Horticulture, Chiba University, 648 Matsudo, Chiba 271-8510, Japan.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Xia
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Tsubokura
authors.forename: Masako
authors.initials: M
authors.lastname: Hoshi
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hanawa
authors.forename: Chizuru
authors.initials: C
authors.lastname: Yano
authors.forename: Kayo
authors.initials: K
authors.lastname: Okamura
authors.forename: Talaat A
authors.initials: TA
authors.lastname: Ahmed
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Anai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Watanabe
authors.forename: Masaki
authors.initials: M
authors.lastname: Hayashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawai
authors.forename: Khwaja G
authors.initials: KG
authors.lastname: Hossain
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Masaki
authors.forename: Kazumi
authors.initials: K
authors.lastname: Asai
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamanaka
authors.forename: Nakao
authors.initials: N
authors.lastname: Kubo
authors.forename: Koh-ichi
authors.initials: K
authors.lastname: Kadowaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nagamura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yano
authors.forename: Takuji
authors.initials: T
authors.lastname: Sasaki
authors.forename: Kyuya
authors.initials: K
authors.lastname: Harada
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D018598:Minisatellite Repeats / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013025:Glycine max / Q000235:genetics*