Use of nanoporous alumina surface for desorption electrospray ionization mass spectrometry in proteomic analysis.
This paper presents use of a nanoporous alumina surface for desorption electrospray ionization mass spectrometry (DESI MS). The DESI MS performance of the nanoporous alumina surface is compared with that of polymethylmethacrylate (PMMA), polytetrafluroethylene (PTFE) and glass, which are popular surfaces in DESI MS experiments. Optimized operating conditions were determined for each of these surfaces by studying the effects of flow rate, tip to surface and surface to MS capillary distance, and spray angle on the DESI MS performance. The analytes (reserpine and BSA tryptic digest) were analyzed on all the surfaces. The results show that the nanoporous alumina surface offers higher ion intensity and increased peptide detection as compared to the other surfaces. Additionally, comparison of ion intensities obtained from the nanoporus alumina and an alumina film confirms that improved performance is due to the inherent nature of the nanostructured surface. Limits of detection (LODs) were determined for the analytes on all the surfaces. It was observed that the nanoporous alumina surface offers improved limits of detection as compared to other surfaces. Another advantage of the nanoporous alumina surface is that it provides to faster analysis associated with rapid drying of liquid samples on the surface. Additionally, porous alumina surface can be used as a dual ionization platform for combined DESI/LDI analysis for further improved peptide detection in proteomic analysis.
Sen
A K
AK
Department of Mechanical Engineering, University of South Carolina, Columbia, SC 29208, USA.
Nayak
R
R
Darabi
J
J
Knapp
D R
DR
D000537:Aluminum Oxide / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D005898:Glass / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011138:Polytetrafluoroethylene / Q000737:chemistry; D016062:Porosity; D020543:Proteome / Q000032:analysis*; D012110:Reserpine / Q000032:analysis; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D011108:Polymers; D011109:Polymethacrylic Acids; D020543:Proteome; C030613:polymethacrylic acid; D012710:Serum Albumin, Bovine; D012110:Reserpine; D011138:Polytetrafluoroethylene; D014357:Trypsin; D000537:Aluminum Oxide