title: Systemic transmigration of allosensitizing donor dendritic cells to host secondary lymphoid organs after rat liver transplantation.
abstract: Donor dendritic cell (DC) migration and allosensitization in host secondary lymphoid organs after liver transplantation are ill defined. We used rat models to investigate graft-derived cells and intrahost allosensitization. Liver transplantation induced diffuse blood-borne migration of donor major histocompatibility class II antigen-positive (MHCII(+)) cells and MHCI(+) cells from the graft to host secondary lymphoid organs, not only the spleen, but also lymph nodes and Peyer's patches. The migrated MHCII(+) cells included DCs and some T cells and B cells. The DCs formed clusters with host BrdU(+) cells where they up-regulated CD86(+), and a CD8(+) T cell proliferative response originated within 24 hours after liver transplantation, demonstrating that these DCs can quickly mature and trigger direct allosensitization in host lymphoid organs. Transfer of allogeneic bone marrow cells also induced DC transmigration and a similar host response. In contrast, allogeneic thoracic duct lymph cells contained many fewer transmigrating DCs, and their transfer induced a comparable T cell response but significantly weaker CD8(+) T cell proliferation. Thus, there is a different outcome via the indirect pathway by host DCs that have captured donor alloantigens.
  
  
  CONCLUSIONS
  The rat liver as well as bone marrow contains an immature DC population that can systemically transmigrate through blood vessel walls of the host secondary lymphoid organs, quickly mature, and induce diffuse intrahost CD8(+) T cell responses, which may promote graft rejection.
authors.affiliation: Department of Anatomy (Macro), Dokkyo Medical University and SORST, Tochigi, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Ueta
authors.forename: Changde
authors.initials: C
authors.lastname: Shi
authors.forename: Nobutomo
authors.initials: N
authors.lastname: Miyanari
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Xu
authors.forename: Shu
authors.initials: S
authors.lastname: Zhou
authors.forename: Masaki
authors.initials: M
authors.lastname: Yamashita
authors.forename: Taichi
authors.initials: T
authors.lastname: Ezaki
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Matsuno
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000502:physiology*; D007700:Kinetics; D016031:Liver Transplantation / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D010641:Phenotype; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D013897:Thoracic Duct / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology