An insight into the sialome of the blood-sucking bug Triatoma infestans, a vector of Chagas' disease.
Triatoma infestans is a hemiptera, vector of Chagas' disease that feeds exclusively on vertebrate blood in all life stages. Hematophagous insects' salivary glands (SG) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cDNA library from its SG was randomly sequenced. Also, salivary proteins were submitted to two-dimensional gel (2D-gel) electrophoresis followed by MS analysis. We present the analysis of a set of 1534 (SG) cDNA sequences, 645 of which coded for proteins of a putative secretory nature. Most salivary proteins described as lipocalins matched peptide sequences obtained from proteomic results.
Assumpção
Teresa C F
TC
Laboratory of Host-Parasite Interface, University of Brasília, Brasília-DF 70.910-900, Brazil. tecris@unb.br
Francischetti
Ivo M B
IM
Andersen
John F
JF
Schwarz
Alexandra
A
Santana
Jaime M
JM
Ribeiro
José M C
JM
D000595:Amino Acid Sequence; D000818:Animals; D001081:Apyrase / Q000378:metabolism; D036022:Capsid Proteins / Q000032:analysis; D014355:Chagas Disease / Q000635:transmission; D004251:DNA Transposable Elements; D023082:Defensins / Q000378:metabolism; D020869:Gene Expression Profiling; D015723:Gene Library; D006790:Host-Parasite Interactions / Q000502:physiology; D007295:Inositol Phosphates / Q000378:metabolism; D054834:Lipocalins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009758:Nymph / Q000378:metabolism; D040901:Proteomics; D018035:Receptors, Odorant / Q000378:metabolism; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D015843:Serpins / Q000378:metabolism; D014224:Triatoma / Q000276:immunology / Q000378:metabolism*
D036022:Capsid Proteins; D004251:DNA Transposable Elements; D023082:Defensins; D007295:Inositol Phosphates; D054834:Lipocalins; D018035:Receptors, Odorant; D012471:Salivary Proteins and Peptides; D015843:Serpins; C509988:trialysin protein, Triatoma infestans; D001081:Apyrase