title: Enterochromaffin cell and 5-hydroxytryptamine responses to the same infectious agent differ in Th1 and Th2 dominant environments.
abstract: OBJECTIVE
  5-Hydroxytryptamine (5-HT) released from enterochromaffin cells influences intestinal homeostasis by altering gut physiology and is implicated in the pathophysiology of various gut disorders. The mechanisms regulating 5-HT production in the gut remain unclear. This study investigated the T helper (Th) 1/Th2-based immunoregulation of enterochromaffin cell function and 5-HT production in a model of enteric infection.
  
  
  RESULTS
  Trichuris muris-infected AKR (susceptible to infection and generates Th1 response), BALB/c (resistant to infection and generates Th2 response), Stat4-deficient (impaired in Th1 response) and Stat6-deficient (impaired in Th2 response) mice were investigated to assess enterochromaffin cells, 5-HT and cytokines. In association with the generation of a Th2 response we observed higher enterochromaffin cell numbers and 5-HT content in the colon of BALB/c mice compared with AKR mice. Numbers of enterochromaffin cells and amount of 5-HT were significantly lower in Stat6-deficient mice after infection compared with Stat4-deficient mice. In addition, enterochromaffin cell numbers and 5-HT content were significantly higher after reconstitution of severe combined immunodeficient mice with in-vitro polarised Th2 cells.
  
  
  CONCLUSIONS
  The study demonstrated that enterochromaffin cell and 5-HT responses to the same infectious agent are influenced by Th1 or Th2 cytokine predominance and suggests that the immunological profile of the inflammatory response is important in the regulation of enterochromaffin cell biology in the gut. In addition to new data on enterochromaffin cell function in enteric infection and inflammation, this study provides important information on the immuno-endocrine axis in the gut, which may ultimately lead to improved strategies against gut disorders.
authors.affiliation: Intestinal Diseases Research Program, Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Motomura
authors.forename: J-E
authors.initials: JE
authors.lastname: Ghia
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Akiho
authors.forename: R T
authors.initials: RT
authors.lastname: El-Sharkawy
authors.forename: M
authors.initials: M
authors.lastname: Collins
authors.forename: Y
authors.initials: Y
authors.lastname: Wan
authors.forename: J T
authors.initials: JT
authors.lastname: McLaughlin
authors.forename: W I
authors.initials: WI
authors.lastname: Khan
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D003106:Colon / Q000378:metabolism / Q000473:pathology; D004198:Disease Susceptibility; D004759:Enterochromaffin Cells / Q000378:metabolism / Q000473:pathology*; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016513:Mice, SCID; D012701:Serotonin / Q000378:metabolism*; D013045:Species Specificity; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014257:Trichuriasis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D015847:Interleukin-4; D012701:Serotonin; D007371:Interferon-gamma