Microarray analysis of toxicogenomic effects of triclosan on Staphylococcus aureus.
For the first time, a genome-wide transcriptional analysis was performed to elucidate the cellular response of Staphylococcus aureus to triclosan. Our results indicate that the effects of triclosan are widespread on metabolism, affecting many vital cellular processes. Triclosan downregulated the transcription of genes involved in virulence factor and energy metabolism such as amino acid, carbohydrate, lipid transport, and metabolism, while multidrug resistance genes, coenzyme transport, and metabolism and transcription genes were upregulated. Furthermore, triclosan downregulated the transcription of genes encoding major lipid metabolism enzymes such as 3-hydroxyacyl-CoA dehydrogenase, acetyl-CoA acetyltransferase, acetyl-CoA synthetase, and acetyl-CoA carboxylase, which all play essential roles in S. aureus lipid metabolism. It is interesting to note that the expression of the enoyl-ACP reductase gene, fabI, was not changed after exposure of S. aureus with 0.05 microM triclosan at 10 and 60 min in our study. This work also implies that triclosan may kill S. aureus by interfering with its ability to form cell membranes. Another important implication of our result is that S. aureus may generate resistance factors under triclosan stress.
Jang
Hyeung-Jin
HJ
Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA. jang.hyeungjin@epa.gov
Chang
Matthew Wook
MW
Toghrol
Freshteh
F
Bentley
William E
WE
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D016680:Genome, Bacterial / Q000187:drug effects*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic / Q000187:drug effects; D014260:Triclosan / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D014260:Triclosan