title: Changing profile of rotavirus in Ireland: predominance of P[8] and emergence of P[6] and P[9] in mixed infections.
abstract: Six hundred and thirty three fecal specimens were collected from patients under 6 years, suffering from non-bacterial, putative viral gastroenteritis in the south of Ireland, between 2003 and 2006. Following laboratory identification of rotavirus as the aetiological agent in 558 specimens, reverse transcriptase polymerase chain reaction was employed to amplify the VP7 and VP4 gene segments of 249 and 245 samples, respectively. G and P typing was subsequently carried out on these amplicons. G1 (65.1%), and G3 (16.1%) were found to be the most prevalent circulating G types over the course of the study. Both G2 (1.2%) and G9 (3.6%), were also found to be circulating, however, these types were less frequently detected. Mixed G type infections were found to account for 41 samples (14%). P typing was carried out on 245 samples. P[8] was the most commonly detected P type over the course of the study (93.5%). Both P[6] and P[9], which had not previously been detected in the Irish population, were detected during this investigation. P[6] was detected in both single and mixed P type infections, while P[9] was detected as part of mixed infections only. The key findings of this study were the emergence of P[6] and P[9] as epidemiologically important rotavirus strains in the Irish population. The profile of rotavirus is changing continuously in Ireland, and continued surveillance of the circulating strains is needed to detect the appearance of new strains, or new variants which could escape immune protection induced by an outdated vaccine.
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Ave, Bishopstown, Cork, Ireland.
authors.forename: G
authors.initials: G
authors.lastname: Lennon
authors.forename: N
authors.initials: N
authors.lastname: Reidy
authors.forename: B
authors.initials: B
authors.lastname: Cryan
authors.forename: S
authors.initials: S
authors.lastname: Fanning
authors.forename: H
authors.initials: H
authors.lastname: O'Shea
mesh_terms: D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*