An endophytic fungus from Hypericum perforatum that produces hypericin.
For the first time, an endophytic fungus has been isolated from the stems of the medicinal herb Hypericum perforatum (St. John's Wort). The fungus produced the napthodianthrone derivative hypericin ( 1) in rich mycological medium (potato dextrose broth) under shake flask and bench scale fermentation conditions. Emodin ( 2) was also produced simultaneously by the fungus under the same culture conditions. We propose 2 as the main precursor in the microbial metabolic pathway to 1. The fungus was identified by morphology and authenticated by 28S (LSU) rDNA sequencing. Compounds 1 and 2 were identified by LC-HRMS, LC-MS/MS, and LC-HRMS/MS and confirmed by comparison with authentic standards. In bioassays with a panel of laboratory standard pathogenic control strains, including fungi and bacteria, both fungal 1 and 2 possessed antimicrobial activity comparable to authentic standards. This endophytic fungus has significant scientific and industrial potential to meet the pharmaceutical demands for 1 in a cost-effective, easily accessible, and reproducible way.
Kusari
Souvik
S
Institute of Environmental Research (INFU), University of Dortmund, Otto-Hahn-Strasse 6, D-44221 Dortmund, Germany.
Lamshöft
Marc
M
Zühlke
Sebastian
S
Spiteller
Michael
M
D000873:Anthracenes; D001234:Aspergillus niger / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D004271:DNA, Fungal / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D020902:Hypericum / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification / Q000494:pharmacology
D000873:Anthracenes; D004271:DNA, Fungal; D010569:Perylene; C004965:hypericin