[Establishment of a CXCR4-expressing gastric cancer cell line in nude mice and the effect of AMD 3100 on tumor regression].
OBJECTIVE
CXCR4 plays a central role in cell migration in metastasis and dissemination of cancer. We have recently established a CXCR4-expressing gastric cancer cell line. Then we have prepared a therapeutic model for it, and assessed its usefulness in the in vivo system.


METHODS
Tumors from gastric cancer patients were transplanted into BALB/C (nu/nu) nude mice. After several subcultures in vivo, the tumor cell line was successfully established. In addition, this cell line expressed the CXCR4 receptor. The nude mice bearing these cell line tumors were administered AMD 3100, an inhibitor of the CXCR4 receptor, and its anti-tumor activity was assessed.


CONCLUSIONS
The tumor volume was reduced by 27.6% in mice administered AMD 3100 compared with the control group (p < 0.02). None of the nude mice showed toxic signs of toxicity. Establishment of a CXCR4 expressing human gastric cancer cell line transplantable into nude mice seems to be important in discussing the possibility of an in vivo model of treatment with AMD 3100 in the in vivo system.
Iwanaga
Tomohiro
T
Dept. of Surgery, Tokyo Metropolitan Komagome Hospital.
Iwasaki
Yoshiaki
Y
Ohashi
Manabu
M
Nunobe
Souya
S
Iwagami
Shiro
S
D000818:Animals; D001596:Benzylamines; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000378:metabolism; D000080027:Cyclams; D015870:Gene Expression; D006571:Heterocyclic Compounds / Q000627:therapeutic use*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D023041:Xenograft Model Antitumor Assays
D001596:Benzylamines; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D000080027:Cyclams; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; C088327:plerixafor