title: A genetic selection for Neurospora crassa mutants altered in their light regulation of transcription.
abstract: Transcription of the Neurospora crassa gene con-10 is induced during conidiation and following exposure of vegetative mycelia to light, but light activation is transient due to photoadaptation. We describe mutational analyses of photoadaptation using a N. crassa strain bearing a translational fusion of con-10, including its regulatory region, to a selectable bacterial gene conferring hygromycin resistance (hph). Growth of this strain was sensitive to hygromycin, upon continuous culture in the light. Five mutants were isolated that were resistant to hygromycin when cultured under constant light. Three mutant strains displayed elevated, sustained accumulation of con-10::hph mRNA during continued light exposure, suggesting that they bear mutations that reduce or eliminate the presumed light-dependent repression mechanism that blocks con-10 transcription upon prolonged illumination. These mutations altered photoadaptation for only a specific group of genes (con-10 and con-6), suggesting that regulation of photoadaptation is relatively gene specific. The mutations increased light-dependent mRNA accumulation for genes al-1, al-2, and al-3, each required for carotenoid biosynthesis, resulting in a threefold increase in carotenoid accumulation following continuous light exposure. Identification of the altered gene or genes in these mutants may reveal novel proteins that participate in light regulation of gene transcription in fungi.
authors.affiliation: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Navarro-Sampedro
authors.forename: Charles
authors.initials: C
authors.lastname: Yanofsky
authors.forename: Luis M
authors.initials: LM
authors.lastname: Corrochano
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects / Q000528:radiation effects; D002338:Carotenoids / Q000096:biosynthesis; D002934:Cinnamates / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000528:radiation effects; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects*; D005800:Genes, Fungal; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008027:Light; D009154:Mutation / Q000235:genetics*; D025282:Mycelium / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D009492:Neurospora crassa / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects*; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D020871:RNA Stability / Q000187:drug effects / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012641:Selection, Genetic; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects*
chemical_list: D002934:Cinnamates; D005656:Fungal Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D002338:Carotenoids; D006921:Hygromycin B; C026273:hygromycin A