title: Elevated atmospheric CO2 affects soil microbial diversity associated with trembling aspen.
abstract: The effects of elevated atmospheric CO(2) (560 p.p.m.) and subsequent plant responses on the soil microbial community composition associated with trembling aspen was assessed through the classification of 6996 complete ribosomal DNA sequences amplified from the Rhinelander WI free-air CO(2) and O(3) enrichment (FACE) experiments microbial community metagenome. This in-depth comparative analysis provides an unprecedented, detailed and deep branching profile of population changes incurred as a response to this environmental perturbation. Total bacterial and eukaryotic abundance does not change; however, an increase in heterotrophic decomposers and ectomycorrhizal fungi is observed. Nitrate reducers of the domain bacteria and archaea, of the phylum Crenarchaea, potentially implicated in ammonium oxidation, significantly decreased with elevated CO(2). These changes in soil biota are evidence for altered interactions between trembling aspen and the microorganisms in its surrounding soil, and support the theory that greater plant detritus production under elevated CO(2) significantly alters soil microbial community composition.
authors.affiliation: Brookhaven National Laboratory, Biology Department, Building 463, Upton, New York 11973-5000, USA.
authors.forename: Celine
authors.initials: C
authors.lastname: Lesaulnier
authors.forename: Dimitris
authors.initials: D
authors.lastname: Papamichail
authors.forename: Sean
authors.initials: S
authors.lastname: McCorkle
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.forename: Steven
authors.initials: S
authors.lastname: Skiena
authors.forename: Safiyh
authors.initials: S
authors.lastname: Taghavi
authors.forename: Donald
authors.initials: D
authors.lastname: Zak
authors.forename: Daniel
authors.initials: D
authors.lastname: van der Lelie
mesh_terms: D000389:Air Conditioning; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001272:Atmosphere; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D016133:Polymerase Chain Reaction; D032107:Populus; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D012987:Soil; D002245:Carbon Dioxide