title: The effects of intra-rectal and intra-peritoneal application of Origanum onites L. essential oil on 2,4,6-trinitrobenzenesulfonic acid-induced colitis in the rat.
abstract: The aim of the present study is to investigate the treatment efficiency of intra-rectal (IR) and intra-peritoneal (IP) application of Origanum onites essential oil (OOEO), which is a well-known antioxidant, in the colitis model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and ethanol (E) in comparison with dexamethasone therapy through the morphologic damage score. Monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1, CD54), anti-rat granulocytes, and myeloperoxidase (MPO), were also investigated immunohistochemically. There was a significant difference in terms of ulceration, mucus cell depletion, inflammatory cell infiltration, vascular dilatation (p<0.001), crypt abscesses (p<0.01), and edema (p<0.05) between OOEO-1mg/kg-IR and control colitis groups. A significant difference was encountered in terms of mucus cell depletion, crypt abscesses, inflammatory cell infiltration, vascular dilatation (p<0.01), and ulceration (p<0.05) between the OOEO-0.1mg/kg-IR and control colitis groups. A significant difference was noticed in terms of ulceration, inflammatory cell infiltration, mucus cell depletion (p<0.001), vascular dilatation (p<0.01), and mucosal atrophy (p<0.05) between the OOEO-1mg/kg-IP and control colitis groups. There was a significant difference in terms of ulceration, mucus cell depletion, inflammatory cell infiltration (p<0.001), crypt abscesses, vascular dilatation (p<0.01), and mucosal atrophy (p<0.05) between the OOEO-0.1mg/kg-IP and control colitis groups. No significant difference was determined in terms of ulceration, inflammatory cyst, mucosal atrophy, edema, and vascular dilatation between the dexamethazone and control colitis groups (p>0.05). Under the present conditions, we concluded that IR and IP OOEO treatment, applied at the dosage of 0.1 or 1mg/kg/day, have a significant protective effect on the colonic injury.
authors.affiliation: Department of Pathology, Eskisehir Osmangazi University Faculty of Medicine, Eskisehir, Turkey. edundar_99@yahoo.com
authors.forename: Emine
authors.initials: E
authors.lastname: Dundar
authors.forename: Esra Gurlek
authors.initials: EG
authors.lastname: Olgun
authors.forename: Serap
authors.initials: S
authors.lastname: Isiksoy
authors.forename: Mine
authors.initials: M
authors.lastname: Kurkcuoglu
authors.forename: K Husnu Can
authors.initials: KH
authors.lastname: Baser
authors.forename: Cengiz
authors.initials: C
authors.lastname: Bal
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D003106:Colon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity; D008401:Gas Chromatography-Mass Spectrometry; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009822:Oils, Volatile / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D031341:Origanum / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D009822:Oils, Volatile; D018799:Intercellular Adhesion Molecule-1; C029292:origanum oil; D000431:Ethanol; D003907:Dexamethasone; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase