Oral insulin delivery by means of solid lipid nanoparticles.
The aim of this work was to produce and characterize cetyl palmitate-based solid lipid nanoparticles (SLN) containing insulin, and to evaluate the potential of these colloidal carriers for oral administration. SLN were prepared by a modified solvent emulsification-evaporation method based on a w/o/w double emulsion. The particle size, zeta potential and association efficiency of unloaded and insulin-loaded SLN were determined and were found to be around 350 nm, negatively charged and the insulin association efficiency was over 43%. After oral administration of insulin-loaded SLN to diabetic rats, a considerable hypoglycemic effect was observed during 24 hours. These results demonstrated that SLN promote the oral absorption of insulin.
Sarmento
Bruno
B
Department of Pharmaceutical Technology, Faculty of Pharmacy University of Porto, Porto, Portugal. bruno.sarmento@ff.up.pt
Martins
Susana
S
Ferreira
Domingos
D
Souto
Eliana B
EB
D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003920:Diabetes Mellitus / Q000097:blood* / Q000188:drug therapy*; D004337:Drug Carriers / Q000737:chemistry*; D007328:Insulin / Q000008:administration & dosage*; D008297:Male; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010168:Palmitates / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
D001786:Blood Glucose; D004337:Drug Carriers; D007328:Insulin; D010168:Palmitates; C006957:cetyl palmitate