title: Phenylalanine requirement, imbalance, and dietary excess in one-week-old chicks: growth and phenylalanine hydroxylase activity.
abstract: Two experiments were performed to study Phe imbalance and toxicity in 1-wk-old Babcock B380 chicks resulting from the addition of either a mixture of indispensable amino acids lacking Phe (IAA - Phe) or excess Phe to a diet that was nutritionally adequate in Phe. Chicks received a preexperimental semipurified diet for 1 wk and experimental diets from 7 to 14 d of age. In the first experiment, the chicks were given diets with Phe levels at 0.24, 0.29, 0.34, 0.39, 0.44, and 0.49% of the diet to determine the Phe requirement. The requirement of the chicks for Phe, based on weight gain and feed efficiency, was determined to be 0.39% of the diet. In experiment 2, the IAA - Phe (10% of the diet) or excess Phe (2% of the diet) was added to a diet containing 0.44% Phe. Chicks given the IAA - Phe or excess Phe had significantly slower growth rates than chicks given the basal diet (P > or = 0.05). The activities of the major hepatic enzyme of Phe catabolism, Phe hydroxylase (PAH), were significantly higher than that of chicks fed the basal diet when the chicks were fed the diets containing IAA - Phe plus 1.1% Phe (P > or = 0.05) but not when chicks were fed the diet containing IAA - Phe alone. The activity of PAH in chicks given the excess (2%) Phe was nearly 4 times the activity of PAH in chicks given the basal diet. Adding IAA - Phe to the diet containing excess Phe also resulted in higher PAH activity than was observed in chicks fed the basal diet, although the activity was significantly lower than observed for chicks receiving the diet containing excess Phe alone (P > or = 0.05). It is concluded that hepatic PAH activity in chicks increases primarily in response to its substrate, Phe. A dietary amino acid load without Phe reduces this response to excess Phe.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, NY 14853, USA. fml5@cornell.edu
authors.forename: F M
authors.initials: FM
authors.lastname: Lartey
authors.forename: R E
authors.initials: RE
authors.lastname: Austic
mesh_terms: D000375:Aging; D000818:Animals; D002645:Chickens / Q000254:growth & development* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009751:Nutritional Requirements; D010649:Phenylalanine / Q000378:metabolism* / Q000494:pharmacology*; D010651:Phenylalanine Hydroxylase / Q000378:metabolism*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D010649:Phenylalanine; D010651:Phenylalanine Hydroxylase