title: Bone tissue reaction of nano-hydroxyapatite/collagen composite at the early stage of implantation.
abstract: The purpose of this study was to develop a new biodegradable bone substitute materials consisting of synthesized nano-size hydroxyapatite (nano-HAp) and Type I biodegradable honeycomb collagen sponge (HCS) composites. Bone defects in rabbit mandibles were prepared by a drill, and the composites were implanted into the bone defects. The HCS only and the HCS/calcined hydroxyapatite (HAp) composite were used as comparative materials. The bone tissues reaction at the early stage within 3 weeks after implantaion was investigated histologically. Amounts of new bone formation were determined by NIH-image analysis software using the histological sections. The amounts of the new bone formation were largest in the nano HAp/HCS compared to the comparative materials. Within 2 weeks after implantation, the nano-HAp/HCS composite was more rapidly exchanged by new bone than the comparative materials. From these results it was considered that the nano-HAp/HCS composites can be used as an effective biodegradable bone substitutive material.
authors.affiliation: Department of Oral Health, School of Life Dentistry at Tokyo, The Nippon Dental University, Chiyoda-ku, Tokyo, Japan. naoto201@a.toshima.ne.jp
authors.forename: N
authors.initials: N
authors.lastname: Fukui
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Kuboki
authors.forename: H
authors.initials: H
authors.lastname: Aoki
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D003094:Collagen / Q000008:administration & dosage* / Q000737:chemistry; D017886:Durapatite / Q000008:administration & dosage* / Q000737:chemistry; D008337:Mandibular Fractures / Q000473:pathology* / Q000601:surgery*; D008422:Materials Testing; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D003094:Collagen; D017886:Durapatite