Characterization of the gene delivery properties of baculoviral-based virosomal vectors.
The current study reports the production of baculoviral-virosomal vectors consisting of lipoplexes and of the viral glycoprotein (GP64) of baculovirus Autographa californica multiple nucleopolyhdrovirus (AcMNPV). This study demonstrates that such complexes have an increased transfection capability in a number of cells, including undifferentiated H9 human embryonic stem H9hES cells compared to lipoplexes alone. The GP64-mediated enhancement of gene transfer of lipoplexes is inhibited by the addition of anti-GP64 neutralizing antibody and by a modified GP64 protein, but is however less potent than vesicular stomatitis virus glycoprotein (VSV-G)-mediated enhancement of gene transfer of lipoplexes. This difference may be explained in part by the dissimilarity in the fusogenic properties of their respective viral glycoprotein.
Guibinga
Ghiabe-Henri
GH
Department of Pediatrics, Center for Molecular Genetics, University of California San Diego School of Medicine, La Jolla, CA 92093-0634, USA. gguibinga@ucsd.edu <gguibinga@ucsd.edu>
Song
Shaochun
S
Loring
Jeanne
J
Friedmann
Theodore
T
D002460:Cell Line; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000378:metabolism; D022701:Virosomes / Q000096:biosynthesis*
C028391:G protein, vesicular stomatitis virus; D008081:Liposomes; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D022701:Virosomes