title: Estimation of apoptosis and cell proliferation in histiocytic necrotizing lymphadenitis using immunohistochemical double staining.
abstract: The aim of the present study was to estimate the relationship between apoptosis and cell proliferation in histiocytic necrotizing lymphadenitis (HNL). Fifteen patients with HNL were retrospectively analyzed. The patients were divided into three groups according to the proportion of the necrotic area as follows: necrosis (+), necrotic area <25%; necrosis (++), necrotic area 25-50%; and necrosis (+++), necrotic area >50%. Immunohistochemical double staining was performed for CD3 plus caspase-3 and for Ki-67 plus caspase-3 and positive cells were counted in two areas: one without and one with obvious apoptotic features. Most caspase-3-positive cells were also stained for CD3 (area exhibiting obvious apoptotic features: average, 92.3%). Furthermore, various proportions of both Ki-67- and caspase-3-positive cells were detected in all the groups (range, 5-70%). In the area with obvious apoptotic changes, the average percentage of both Ki-67- and caspase-3-positive cells (38.6%) was higher than that in the area without obvious apoptotic features (16.3%). A proportion of cells in HNL undergo proliferation and apoptosis simultaneously, such as neoplastic cells, thereby exhibiting rapid cell cycles.
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan. yuko-nomura@mvj.biglobe.ne.jp
authors.forename: Yuko
authors.initials: Y
authors.lastname: Nomura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sugita
authors.forename: Shiro
authors.initials: S
authors.lastname: Yoshida
authors.forename: Kennosuke
authors.initials: K
authors.lastname: Karube
authors.forename: Kei
authors.initials: K
authors.lastname: Shimizu
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Aoki
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hirose
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
mesh_terms: D000328:Adult; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D002452:Cell Count; D049109:Cell Proliferation; D002648:Child; D005260:Female; D020042:Histiocytic Necrotizing Lymphadenitis / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D019394:Ki-67 Antigen / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009336:Necrosis; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; D019394:Ki-67 Antigen; D053148:Caspase 3