title: Major uropathogenic Escherichia coli strain isolated in the northwest of England identified by multilocus sequence typing.
abstract: A total of 88 uropathogenic Escherichia coli isolates, including 68 isolates from urine and 20 isolates from blood, were characterized by multilocus sequence typing (MLST). MLST has identified an important genetic lineage of E. coli, designated sequence type 131 (ST-131), represented by 52 of these isolates, 51 of which were resistant to extended-spectrum cephalosporins. ST-131 appears to be a drug-resistant uropathogenic strain of E. coli responsible for causing urinary tract infections and bacteremia and is widely disseminated among both community and hospital patients from different geographical areas in the northwest of England. Application of MLST has helped to define the population biology which may underpin the epidemiology of pathogenic E. coli strains. The portability of MLST allows the accurate monitoring of this antibiotic-resistant uropathogenic strain of E. coli and will enhance surveillance for this important group of organisms.
authors.affiliation: Department of Medical Microbiology, School of Medicine, University of Manchester, Clinical Sciences Building 1, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, United Kingdom.
authors.forename: Siu Ha
authors.initials: SH
authors.lastname: Lau
authors.forename: Suganya
authors.initials: S
authors.lastname: Reddy
authors.forename: John
authors.initials: J
authors.lastname: Cheesbrough
authors.forename: Frederick J
authors.initials: FJ
authors.lastname: Bolton
authors.forename: Geraldine
authors.initials: G
authors.lastname: Willshaw
authors.forename: Tom
authors.initials: T
authors.lastname: Cheasty
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Fox
authors.forename: Mathew
authors.initials: M
authors.lastname: Upton
mesh_terms: D016470:Bacteremia / Q000453:epidemiology / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D018550:Cephalosporin Resistance; D004739:England / Q000453:epidemiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D017422:Sequence Analysis, DNA; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology; D014556:Urine / Q000382:microbiology
chemical_list: D029968:Escherichia coli Proteins