Crystallization and X-ray analysis of Rhodothermus marinus cytochrome c at 1.23 A resolution.
Cytochrome c from Rhodothermus marinus has been crystallized using the hanging-drop vapor-diffusion method in 30 % (w/v) polyethylene glycol 8K, 0.2 M ammonium sulfate, 8 % hexanediol and 50 mM sodium citrate pH 2.2. The crystals belong to space group P2(1). X-ray diffraction data were collected to 1.23 A resolution using synchrotron radiation and a wavelength of 0.93 A.
Stelter
Meike
M
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2781-901 Oeiras, Portugal.
Melo
Ana M P
AM
Saraiva
Lígia M
LM
Teixeira
Miguel
M
Archer
Margarida
M
D003460:Crystallization; D018360:Crystallography, X-Ray; D045304:Cytochromes c / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols / Q000737:chemistry; D045245:Rhodothermus / Q000201:enzymology*; D017356:Synchrotrons
D011092:Polyethylene Glycols; D045304:Cytochromes c