title: Matrix metalloproteinase-9 associated with heparan sulphate chains of GPI-anchored cell surface proteoglycans mediates motility of murine colon adenocarcinoma cells.
abstract: Using murine colon adenocarcinoma-derived clones with different metastatic potentials, the cellular localization of matrix metalloporteinase-9 (MMP-9) and its role in the cell motility were examined. Highly metastatic LuM1 clone aggressively invaded into adjacent tissue in vivo, but low metastatic NM11 clone did not. As compared with the NM11 clone, the LuM1 clone expressed and secreted a remarkably large amount of MMP-9, and exhibited higher abilities of cell migration and invasion in vitro, which were suppressed by MMP-2/MMP-9 inhibitor IV. MMP-9, exhibiting high affinity to heparin, was demonstrated to be condensed on tips of cellular podia. Treatment of the cells with heparitinase-I or heparin resulted in release of MMP-9 from the cell surface, which caused concomitant suppression of their motility to a similar level to that with the MMP inhibitor. Immunoprecipitation of a LuM1 cell lysate with an anti-MMP-9 antibody resulted in co-precipitation of phosphatidylinositol-specific phospholipase C-susceptible heparan sulphate proteoglycans having 66 and 64 kDa core proteins. Taken together, the present results demonstrate that secreted MMP-9 associates with glypican-like proteoglycans through their heparan sulphate chains, and plays a crucial role in cell motility of LuM1 cells.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kamigamo Motoyama, Kitaku, Kyoto 603-8555, Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Koyama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Naruo
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshitomi
authors.forename: Seiichi
authors.initials: S
authors.lastname: Munesue
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Kiyono
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kusano
authors.forename: Katsunori
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Toyoharu
authors.initials: T
authors.lastname: Yokoi
authors.forename: Hayao
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Satoru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Minoru
authors.initials: M
authors.lastname: Okayama
authors.forename: Kayoko
authors.initials: K
authors.lastname: Oguri
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000556:secondary; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology*; D002465:Cell Movement; D002999:Clone Cells; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism
chemical_list: D017261:Glycosylphosphatidylinositols; D019812:Heparan Sulfate Proteoglycans; D008565:Membrane Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D020780:Matrix Metalloproteinase 9