title: Enamel subsurface lesion remineralisation with casein phosphopeptide stabilised solutions of calcium, phosphate and fluoride.
abstract: Casein phosphopeptide stabilised amorphous calcium phosphate (CPP-ACP) and amorphous calcium fluoride phosphate (CPP-ACFP) solutions have been shown to remineralise enamel subsurface lesions. The aim of this study was to determine the effect of ion composition of CPP-ACP and CPP-ACFP solutions on enamel subsurface lesion remineralisation in vitro. CPP-bound and free calcium, phosphate and fluoride ion concentrations in the solutions were determined after ultrafiltration. The ion activities of the free ion species present were calculated using an iterative computational program. The mineral deposited in the subsurface lesions was analysed using transverse microradiography and electron microprobe. CPP was found to stabilise high concentrations of calcium, phosphate and fluoride ions at all pH values (7.0-4.5). Remineralisation of the subsurface lesions was observed at all pH values tested with a maximum at pH 5.5. The CPP-ACFP solutions produced greater remineralisation than the CPP-ACP solutions at pH 5.5 and below. The mineral formed in the subsurface lesions was consistent with hydroxyapatite and fluorapatite for remineralisation with CPP-ACP and CPP-ACFP, respectively. The activity gradient of the neutral ion pair CaHPO(4)(0) into the lesion was significantly correlated with remineralisation and together with HF(0) were identified as important species for diffusion.
authors.affiliation: Cooperative Research Centre for Oral Health Science, School of Dental Science, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, Parkville, Vic., Australia.
authors.forename: N J
authors.initials: NJ
authors.lastname: Cochrane
authors.forename: S
authors.initials: S
authors.lastname: Saranathan
authors.forename: F
authors.initials: F
authors.lastname: Cai
authors.forename: K J
authors.initials: KJ
authors.lastname: Cross
authors.forename: E C
authors.initials: EC
authors.lastname: Reynolds
mesh_terms: D002118:Calcium / Q000032:analysis; D002327:Cariostatic Agents / Q000627:therapeutic use*; D002364:Caseins / Q000627:therapeutic use*; D003743:Dental Enamel / Q000737:chemistry; D004577:Electron Probe Microanalysis; D005459:Fluorides / Q000032:analysis / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010710:Phosphates / Q000032:analysis; D014089:Tooth Remineralization / Q000379:methods*
chemical_list: D002327:Cariostatic Agents; D002364:Caseins; D010710:Phosphates; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D005459:Fluorides; D002118:Calcium