Disruption of quinoprotein ethanol dehydrogenase gene and adjacent genes in Pseudomonas putida HK5.
Pseudomonas putida HK5 produces three different quinoprotein alcohol dehydrogenases: ADH-I, ADH-IIB and ADH-IIG. Gene organization of qedA, the gene for ADH-I, and other 10 genes in the cluster was related to the genome sequences of five other Pseudomonas strains. Insertion mutations in either qedA, exaE or agmR eliminated ADH-I activity, although the mutants were still able to grow on ethanol but more slowly than the wild-type strain. Mutant analysis demonstrated the requirement of agmR and exaE in ADH-I expression, and the tentative involvement of agmR, but not exaE, in the induction of ADH-IIB and ADH-IIG activities.
Promden
Worrawat
W
Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Vangnai
Alisa S
AS
Pongsawasdi
Piamsook
P
Adachi
Osao
O
Matsushita
Kazunobu
K
Toyama
Hirohide
H
D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005798:Genes, Bacterial; D016958:Pseudomonas putida / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*
D000431:Ethanol; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)