Characterized chondrocyte implantation results in better structural repair when treating symptomatic cartilage defects of the knee in a randomized controlled trial versus microfracture.
BACKGROUND
As the natural healing capacity of damaged articular cartilage is poor, joint surface injuries are a prime target for regenerative medicine. Characterized chondrocyte implantation uses an autologous cartilage cell therapy product that has been optimized for its biological potency to form stable cartilage tissue in vivo.


OBJECTIVE
To determine whether, in symptomatic cartilage defects of the femoral condyle, structural regeneration with characterized chondrocyte implantation is superior to repair with microfracture.


METHODS
Randomized controlled trial; Level of evidence, 1.


METHODS
Characterized chondrocyte implantation was compared with microfracture in patients with single grade III to IV symptomatic cartilage defects of the femoral condyles in a multicenter trial. Patients aged 18 to 50 years were randomized to characterized chondrocyte implantation (n = 57) or microfracture (n = 61). Structural repair was blindly assessed in biopsy specimens taken at 1 year using (1) computerized histomorphometry and (2) evaluation of overall histological components of structural repair. Clinical outcome was measured using the self administered Knee injury and Osteoarthritis Outcome Score. Adverse events were recorded throughout the study.


RESULTS
Characterized chondrocyte implantation resulted in better structural repair, as assessed by histomorphometry (P = .003) and overall histologic evaluation (P = .012). Aspects of structural repair relating to chondrocyte phenotype and tissue structure were superior with characterized chondrocyte implantation. Clinical outcome as measured by the Knee injury and Osteoarthritis Outcome Score at 12 to 18 months after characterized chondrocyte implantation was comparable with microfracture at this stage. Both treatment groups had a similar mean baseline overall Knee injury and Osteoarthritis Outcome Score (56.30 +/- 13.61 and 59.53 +/- 14.95 for microfracture and characterized chondrocyte implantation, respectively), which increased in both groups to 70.56 +/- 12.39 and 72.63 +/- 15.55 at 6 months, 73.26 +/- 14.66 and 73.10 +/- 16.01 at 12 months, and 74.73 +/- 17.01 and 75.04 +/- 14.50 at 18 months, respectively. Both techniques were generally well tolerated; the incidence of adverse events after characterized chondrocyte implantation was not markedly increased compared with that for microfracture.


CONCLUSIONS
One year after treatment, characterized chondrocyte implantation was associated with a tissue regenerate that was superior to that after microfracture. Short-term clinical outcome was similar for both treatments. The superior structural outcome may result in improved long-term clinical benefit with characterized chondrocyte implantation. Long-term follow-up is needed to confirm these findings.
Saris
Daniel B F
DB
Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands. d.saris@umcutrecht.nl
Vanlauwe
Johan
J
Victor
Jan
J
Haspl
Miroslav
M
Bohnsack
Michael
M
Fortems
Yves
Y
Vandekerckhove
Bruno
B
Almqvist
K Frederik
KF
Claes
Toon
T
Handelberg
Frank
F
Lagae
Koen
K
van der Bauwhede
Jan
J
Vandenneucker
Hilde
H
Yang
K Gie Auw
KG
Jelic
Mislav
M
Verdonk
Rene
R
Veulemans
Nancy
N
Bellemans
Johan
J
Luyten
Frank P
FP
D000328:Adult; D054544:Arthroplasty, Subchondral; D001707:Biopsy, Needle; D002358:Cartilage, Articular / Q000293:injuries / Q000473:pathology / Q000601:surgery*; D019902:Chondrocytes / Q000637:transplantation*; D005260:Female; D006801:Humans; D007718:Knee Injuries / Q000601:surgery; D008297:Male; D011446:Prospective Studies; D012038:Regeneration; D016896:Treatment Outcome