Functional and structural roles of conserved cysteine residues in the carboxyl-terminal domain of the follicle-stimulating hormone receptor in human embryonic kidney 293 cells.
The carboxyl-terminal segment of G protein-coupled receptors has one or more conserved cysteine residues that are potential sites for palmitoylation. This posttranslational modification contributes to membrane association, internalization, and membrane targeting of proteins. In contrast to other members of the glycoprotein hormone receptor family (the LH and thyroid-stimulating hormone receptors), it is not known whether the follicle-stimulating hormone receptor (FSHR) is palmitoylated and what are the effects of abolishing its potential palmitoylation sites. In the present study, a functional analysis of the FSHR carboxyl-terminal segment cysteine residues was carried out. We constructed a series of mutant FSHRs by substituting cysteine residues with alanine, serine, or threonine individually and together at positions 629 and 655 (conserved cysteines) and 627 (nonconserved). The results showed that all three cysteine residues are palmitoylated but that only modification at Cys629 is functionally relevant. The lack of palmitoylation does not appear to greatly impair coupling to G(s) but, when absent at position 629, does significantly impair cell surface membrane expression of the partially palmitoylated receptor. All FSHR Cys mutants were capable of binding agonist with the same affinity as the wild-type receptor and internalizing on agonist stimulation. Molecular dynamics simulations at a time scale of approximately 100 nsec revealed that replacement of Cys629 resulted in structures that differed significantly from that of the wild-type receptor. Thus, deviations from wild-type conformation may potentially contribute to the severe impairment in plasma membrane expression and the modest effects on signaling exhibited by the receptors modified in this particular position.
Uribe
Aída
A
Research Unit in Reproductive Medicine, Hospital de Ginecobstetricia "Luis Castelazo Ayala," Instituto Mexicano del Seguro Social, Mexico.
Zariñán
Teresa
T
Pérez-Solis
Marco A
MA
Gutiérrez-Sagal
Rubén
R
Jardón-Valadez
Eduardo
E
Piñeiro
Angel
A
Dias
James A
JA
Ulloa-Aguirre
Alfredo
A
D000409:Alanine / Q000032:analysis / Q000502:physiology; D000595:Amino Acid Sequence; D002460:Cell Line; D003198:Computer Simulation; D000242:Cyclic AMP / Q000378:metabolism; D003545:Cysteine / Q000032:analysis* / Q000502:physiology*; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D054878:Lipoylation / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011962:Receptors, FSH / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D012694:Serine / Q000032:analysis / Q000502:physiology; D013912:Threonine / Q000032:analysis / Q000502:physiology
D011962:Receptors, FSH; D013912:Threonine; D012694:Serine; D000242:Cyclic AMP; D003545:Cysteine; D000409:Alanine