title: A high-fructose diet impairs basal and stress-mediated lipid metabolism in healthy male subjects.
abstract: The effects of a 7 d high-fructose diet (HFrD) or control diet on lipid metabolism were studied in a group of six healthy lean males. Plasma NEFA and beta-hydroxybutyrate concentrations, net lipid oxidation (indirect calorimetry) and exogenous lipid oxidation (13CO2 production) were monitored in basal conditions, after lipid loading (olive oil labelled with [13C]triolein) and during a standardised mental stress. Lactate clearance and the metabolic effects of an exogenous lactate infusion were also monitored. The HFrD lowered plasma concentrations of NEFA and beta-hydroxybutyrate as well as lipid oxidation in both basal and after lipid-loading conditions. In addition, the HFrD blunted the increase in plasma NEFA and exogenous lipid oxidation during mental stress. The HFrD also increased basal lactate concentrations by 31.8 %, and lactate production by 53.8 %, while lactate clearance remained unchanged. Lactate infusion lowered plasma NEFA with the control diet, and net lipid oxidation with both the HFrD and control diet. These results indicate that a 7 d HFrD markedly inhibits lipolysis and lipid oxidation. The HFrD also increases lactate production, and the ensuing increased lactate utilisation may contribute to suppress lipid oxidation.
authors.affiliation: Department of Physiology, School of Biology and Medicine, University of Lausanne, 7 rue du Bugnon, Lausanne CH-1005, Switzerland.
authors.forename: Andrew
authors.initials: A
authors.lastname: Abdel-Sayed
authors.forename: Christophe
authors.initials: C
authors.lastname: Binnert
authors.forename: Kim-Anne
authors.initials: KA
authors.lastname: Lê
authors.forename: Murielle
authors.initials: M
authors.lastname: Bortolotti
authors.forename: Philippe
authors.initials: P
authors.lastname: Schneiter
authors.forename: Luc
authors.initials: L
authors.lastname: Tappy
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000328:Adult; D001481:Basal Metabolism / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D004032:Diet; D004734:Energy Metabolism / Q000187:drug effects; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005632:Fructose / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D009747:Nutritional Physiological Phenomena; D013315:Stress, Psychological / Q000097:blood*
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D005632:Fructose; D019344:Lactic Acid; D020155:3-Hydroxybutyric Acid