title: Altered malondialdehyde, protein carbonyl and sialic acid levels in seminal plasma of microscopically abnormal semen.
abstract: Microscopically abnormal (n = 26) semen showed significantly higher levels of malondialdehyde (MDA), protein carbonyl (PC) and protein-bound sialic acid (SA) in seminal plasma as compared with normal semen (n = 24). The percentage of nonmotile spermatozoa showed significant (P < 0.01) positive correlation with MDA (r = 0.5) and PC (r = 0.49). Sperm counts showed a significant negative correlation with MDA (r = -0.63, P < 0.001) level of seminal plasma. SA correlated (r = 0.56, P < 0.01) with MDA. The receiver operating characteristic (ROC) curve of MDA and SA showed that MDA of 3.15 micromol l(-1) and SA of 3.85 micromol l(-1) were optimum cut-off limits to discriminate abnormal semen from normal. In conclusion, high SA might be a protective response against prevailed oxidative stress in abnormal semen. Seminal plasma MDA and SA may act as potential markers of abnormal semen.
authors.affiliation: Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education & Research, Pondicherry, India.
authors.forename: A
authors.initials: A
authors.lastname: Saraniya
authors.forename: B C
authors.initials: BC
authors.lastname: Koner
authors.forename: P
authors.initials: P
authors.lastname: Doureradjou
authors.forename: N
authors.initials: N
authors.lastname: Selvaraj
authors.forename: V
authors.initials: V
authors.lastname: Shivagourou
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D050050:Protein Carbonylation / Q000502:physiology*; D012661:Semen / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D019158:N-Acetylneuraminic Acid