title: Antifungal activities of bronopol and 2-methyl-4-isothiazolin-3-one (MT) against saprolegnia.
abstract: This study compared the antifungal activities of bronopol and 2-methyl-4-isothiazolin-3-one (MT) against members of the genus Saprolegnia in the family saprolegniaceae, which have the ability to infect fish. The minimum inhibitory concentration of bronopol was 100-200 mg/l but for MT it was 31 mg/l. Concerning fungicidal effects against the hyphae, treatment with bronopol at 100-200 mg/l for 30 min or treatment with MT at 25-50 mg/l for 30 min was effective in killing the vegetative stage of all fungal strains tested. Treatment with bronopol at 100 mg/l for 60 min or treatment with MT at 25-50 mg/l for 60 min was effective in killing the zoospores of all fungal strains tested. These results showed that MT was a more effective antifungal agent than bronopol against infectious members of the genus Saprolegnia.
authors.affiliation: Division of Fish Diseases, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
authors.forename: Heisuke
authors.initials: H
authors.lastname: Oono
authors.forename: Kishio
authors.initials: K
authors.lastname: Hatai
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D011409:Propylene Glycols / Q000494:pharmacology*; D044762:Saprolegnia / Q000187:drug effects*; D013844:Thiazoles / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D011310:Preservatives, Pharmaceutical; D011409:Propylene Glycols; D013844:Thiazoles; C011506:2-methyl-4-isothiazolin-3-one; C006827:bronopol