title: Determination of serum 7-ketocholesterol concentrations and their relationships with coronary multiple risks in diabetes mellitus.
abstract: Oxysterols have cytotoxic effects and contribute to the development of atherosclerosis. To examine association between 7-ketocholesterol and diabetes mellitus, and other coronary risk factors, we developed a reliable quantitative method to measure serum 7-ketocholesterol (s-7KCHO) and studied s-7KCHO in patients with type 2 diabetes mellitus (T2DM). The s-7KCHO was detected by gas chromatography-mass spectrometry assay. The s-7KCHO was significantly higher in patients with T2DM (n=137, 33.8 ng/ml) compared to non-diabetic healthy subjects (n=89, 16.1 ng/ml). Patients with T2DM were divided into two groups with two or more than two risk factors (defined as multiple risk factors group) and with zero or one risk factor (non-multiple risk factors group). The s-7KCHO was significantly higher in multiple risk factors group (39.5 ng/ml) compared to non-multiple risk factors (30.1 ng/ml). Among patients with multiple risk factors group, s-7KCHO was significantly higher in patients with high low-density lipoprotein cholesterol (LDL-C) levels (45.1+/-5.9 ng/ml) compared to those with normal LDL-C levels (35.3+/-7.0 ng/ml). Furthermore, s-7KCHO increased according to the number of concurrent coronary risk factors. These results suggest that serum 7-ketocholesterol levels may depend on the multiple risk factors and serum LDL-C levels.
authors.affiliation: The Center of Diabetes, Endocrinology, and Metabolism, Toho University Medical Center Sakura Hospital, 564-1 Shimoshizu, Sakura-City, Chiba, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Endo
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Oyama
authors.forename: Atsuhito
authors.initials: A
authors.lastname: Saiki
authors.forename: Noriko
authors.initials: N
authors.lastname: Ban
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ohira
authors.forename: Nobukiyo
authors.initials: N
authors.lastname: Koide
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Murano
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Manabu
authors.initials: M
authors.lastname: Nishii
authors.forename: Minoru
authors.initials: M
authors.lastname: Miura
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Sekine
authors.forename: Yoh
authors.initials: Y
authors.lastname: Miyashita
authors.forename: Koji
authors.initials: K
authors.lastname: Shirai
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D002624:Chemistry, Clinical / Q000295:instrumentation / Q000592:standards; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood* / Q000453:epidemiology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000453:epidemiology; D003925:Diabetic Angiopathies / Q000097:blood* / Q000453:epidemiology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000592:standards*; D006801:Humans; D007653:Ketocholesterols / Q000032:analysis / Q000097:blood*; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood / Q000453:epidemiology; D008875:Middle Aged; D015203:Reproducibility of Results; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D008078:Cholesterol, LDL; D007653:Ketocholesterols; C003001:7-ketocholesterol