Biocompatibility and membrane strength of C3H10T1/2 cell-loaded alginate-based microcapsules.
BACKGROUND
C3H10T1/2 cells, from a mouse embryonic fibroblast cell line, were used to investigate the improvement of alginate-based microencapsulated cells for cellular therapy.


METHODS
Purified sodium alginate (PSA) and non-purified sodium alginate (SA) were used to prepare alginate-based microcapsules, and their biocompatibility and membrane strength were then compared for the purposes of analyzing the advantages of purifying SA. In addition, poly-l-lysine (PLL) was replaced by chitosan for alginate-chitosan microcapsule preparation. The process of optimization and chemical modification of alginate-chitosan microcapsules using polyethylene glycol was also reviewed.


RESULTS
The results showed improved biocompatibility and membrane strength of PSA-based microcapsules. Under optimal conditions, mesenchymal stromal cell (MSC)-loaded alginate-chitosan microcapsules with good morphology could be obtained using PSA and chitosans of medium molecular weight (1.0-2.5 x 10(5)). A chitosan solution of 0.1% (w/v) and a reaction time of 7 min between alginate and chitosan were determined as optimal preparation parameters.


CONCLUSIONS
It could be concluded that the chemical modification of alginate-based microcapsules can improve their biocompatibility.
Zhang
W-J
WJ
Institute of Cryomedical Technology and Food Freezing, University of Shanghai for Science and Technology, Shanghai, People's Republic of China.
Li
B-G
BG
Zhang
C
C
Xie
X-H
XH
Tang
T-T
TT
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002214:Capsules / Q000737:chemistry*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000737:chemistry / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008567:Membranes, Artificial; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry; D033581:Stem Cell Transplantation / Q000379:methods*
D000464:Alginates; D001672:Biocompatible Materials; D002214:Capsules; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D048271:Chitosan