title: Breed differences in the pharmacokinetics of ivermectin administered subcutaneously to Holstein and Belgian Blue calves.
abstract: Belgian Blue (BB) cattle are very sensitive to mange caused by Psoroptes ovis and, in contrast to the case in Holstein cattle, single treatments with ivermectin do not result in complete elimination of the parasite. The objective of the present study was to determine the concentration of ivermectin in plasma, skin and hair following subcutaneous administration to Holstein and BB calves and to assess the influence of breed on drug pharmacokinetics and availability. Two groups of six healthy female Holstein and BB calves were treated with ivermectin (SC formulation) at a dose of 0.2 mg/kg. Blood, skin and hair were collected before treatment and up to 21 days after treatment. Ivermectin was analyzed in plasma and tissue by high-performance liquid chromatography (HPLC). The peak concentrations (Cmax), time-peak concentrations (Tmax), the area under the plasma concentration-time curves (AUC) and the mean residence time (MRT) were determined. The patterns of plasma and tissue ivermectin concentrations were similar in the two breeds of animals, however, the AUC and Cmax levels for plasma and skin were significantly higher in the BB calves. In hair, ivermectin was detected later than in plasma and skin, with the Tmax ranging between 4 days (Holstein group) and 6 days (BB group). The possible reasons for the significantly higher levels in plasma and skin in BB calves compared to Holstein calves are discussed.
authors.affiliation: Department of Virology, Parasitology and Immunology, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B9820 Merelbeke, Belgium. jozef.vercruysse@ugent.be
authors.forename: Jozef
authors.initials: J
authors.lastname: Vercruysse
authors.forename: Piet
authors.initials: P
authors.lastname: Deprez
authors.forename: Dries
authors.initials: D
authors.lastname: Everaert
authors.forename: Firas
authors.initials: F
authors.lastname: Bassissi
authors.forename: Michel
authors.initials: M
authors.lastname: Alvinerie
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019540:Area Under Curve; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000188:drug therapy*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005260:Female; D006197:Hair / Q000737:chemistry / Q000378:metabolism; D007279:Injections, Subcutaneous / Q000662:veterinary; D007306:Insecticides / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D007559:Ivermectin / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D008924:Mite Infestations / Q000188:drug therapy / Q000662:veterinary*; D040042:Psoroptidae / Q000187:drug effects*; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D007306:Insecticides; D007559:Ivermectin