Comparison analysis of gene expression patterns between sporadic Alzheimer's and Parkinson's disease.
Sporadic Alzheimer's (AD) and Parkinson's disease (PD) are late-onset neurodegenerative diseases with tremendous impact on lives of affected individuals. There is a great probability of developing concurrent Parkinsonism in AD and vice-versa than would be predicted by independent prevalence of each disease. We hypothesize that in sporadic AD as well as PD a combination of environmental effects and gene expression may affect specific brain areas leading to neurodegeneration. We profiled gene expression of AD compared to PD and age matched controls post-mortem in the hippocampus, the gyrus-frontalis-medius (Gfm) and the cerebellum using Gene-Chip microarray (Affymetrix) and quantitative-real-time-RT-PCR. Twelve genes altered in similar manner in AD and PD, while four genes showed differential expression profiles between AD and PD in different brain regions (cannabinoid-receptor-2, Histone-cluster-1-H3e, nicotinic-cholinergic-receptor-alpha6 and beta-site-APP-cleaving enzyme-1). Knowledge of selective gene expression profile can lead to better understanding of disease pathology and development of specific diagnosis and effective therapy.
Grünblatt
Edna
E
Neurochemistry Laboratory, National Parkinson Foundation Centre of Excellence Laboratories, Clinic and Policlinic for Psychiatry and Psychotherapy, University of Würzburg, Germany. Gruenblatt_E@klinik.uni-wuerzburg.de
Zander
Nicole
N
Bartl
Jasmin
J
Jie
Li
L
Monoranu
Camelia-Maria
CM
Arzberger
Thomas
T
Ravid
Rivka
R
Roggendorf
Wolfgang
W
Gerlach
Manfred
M
Riederer
Peter
P
D000368:Aged; D000544:Alzheimer Disease / Q000235:genetics* / Q000473:pathology; D001921:Brain / Q000378:metabolism / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics*; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006824:Hybridization, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010300:Parkinson Disease / Q000235:genetics*; D018075:RNA, Complementary / Q000235:genetics; D043885:Receptor, Cannabinoid, CB2 / Q000235:genetics / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044764:Ubiquitin-Activating Enzymes
C470410:CHRNA6 protein, human; D004268:DNA-Binding Proteins; D006657:Histones; D018075:RNA, Complementary; D043885:Receptor, Cannabinoid, CB2; D011978:Receptors, Nicotinic; D044764:Ubiquitin-Activating Enzymes; C491802:NAE protein, human