Physiological responses of Matricaria chamomilla to cadmium and copper excess.
Physiological responses of Matricaria chamomilla plants exposed to cadmium (Cd) and copper (Cu) excess (3, 60, and 120 microM for 7 days) with special emphasis on phenolic metabolism were studied. Cu at 120 microM reduced chamomile growth, especially in the roots where it was more abundant than Cd. Notwithstanding the low leaf Cu amount (37.5 microg g(-1) DW) in comparison with Cd (237.8 microg g(-1) DW) at 120 microM, it caused reduction of biomass accumulation, F(v)/F(m) ratio and soluble proteins. In combination with high accumulation of phenolics, strong reduction of proteins and high GPX activity in the roots, this supports severe redox Cu properties. In terms of leaf phenylalanine ammonia-lyase (PAL) activity, it seems that Cd had a stimulatory effect during the course of the experiment, whereas Cu was found to stimulate it after 7-day exposure. The opposite trend was visible in the roots, where Cd had a stimulatory effect at high doses but Cu mainly at the highest dose. This supports the assumption of different PAL time dynamics under Cd and Cu excess. A dose of 60 and 120 microM Cu led to 2- and 3-times higher root lignin accumulation while the same Cd doses increased it by 33 and 68%, respectively. A Cu dose of 120 microM can be considered as limiting for chamomile growth under conditions of present research, while resistance to high Cd doses was confirmed. However, PAL and phenolics seemed to play an important role in detoxification of Cd- and Cu-induced oxidative stress.
Kovácik
Jozef
J
Department of Botany, Institute of Biology and Ecology, Faculty of Science, P J Safárik University, 041 67, Kosice, Slovak Republic. jozkovacik@yahoo.com
Backor
Martin
M
Kaduková
Jana
J
D018533:Biomass; D002104:Cadmium / Q000633:toxicity*; D003300:Copper / Q000633:toxicity*; D004781:Environmental Exposure; D006801:Humans; D031168:Matricaria / Q000187:drug effects* / Q000502:physiology; D019216:Metals, Heavy / Q000633:toxicity*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D012989:Soil Pollutants / Q000633:toxicity*
D019216:Metals, Heavy; D012989:Soil Pollutants; D002104:Cadmium; D003300:Copper