Conformational and quantitative characterization of lysozyme extracted from galyfilcon and senofilcon silicone hydrogel contact lenses.
OBJECTIVE
To compare two solvents for retrieval of lysozyme deposited on a silicone hydrogel (SH) contact lens material galyfilcon A (GA; Acuvue Advance).


METHODS
Two buffers used were 50:50 acetonitrile/0.02% trifluoroacetic acid (buffer 1) and 50:50 acetonitrile/50 mM NaOH (buffer 2).


RESULTS
Extraction efficiency from GA lenses was 74% (buffer 1) and 83% (buffer 2). Buffer 2 decreased lysozyme activity > buffer 1. Ex vivo GA lenses showed total protein deposition of 2-16 microg/lens with total lysozyme deposition of 0.3-3.9 microg/lens.


CONCLUSIONS
We have developed a low acid strength extraction buffer that can be used to efficiently extract active lysozyme protein from novel siloxane-based contact lens materials.
Glasier
Mary-Ann
MA
Centre for Contact Lens Research, School of Optometry, University of Waterloo, Waterloo, Ontario, Canada.
Keech
Adam
A
Sheardown
Heather
H
Subbaraman
Lakshman N
LN
Jones
Lyndon
L
D000327:Adsorption; D015153:Blotting, Western; D002021:Buffers; D003263:Contact Lenses, Hydrophilic; D006801:Humans; D020100:Hydrogels; D009113:Muramidase / Q000032:analysis / Q000302:isolation & purification*; D012828:Silicones; D012997:Solvents
D002021:Buffers; D020100:Hydrogels; D012828:Silicones; D012997:Solvents; D009113:Muramidase