title: NE-11 represents a new strain variant class of Potato virus Y.
abstract: This report describes the characterization by whole-genome sequencing of four PVY isolates with unique combinations of molecular and symptomatic characteristics. Three of these four isolates were of type PVY(N:O) (ID-1, OR-1, PN10A), including one of "type B", which contains an extra recombination event in the 5'UTR/P1 cistron; the other (NE-11) represents a novel PVY molecular genotype, previously misclassified as a PVY(NA-NTN) isolate. The full genome sequence of this latter isolate is unique inasmuch as it is nearly identical to that of PVY(N) isolates for the first 2,000 nucleotides (nts), after which it very strongly resembles PVY(NA-NTN) isolates for the next 600 nts. For the final 7,000 nts of its genome, NE-11 shares intermediate identity with these other two previously reported classes of PVY(N) genomes, except for a portion of the capsid protein region in which it resembles neither. Recombination in each of the four isolates was verified by a suite of recombination detection programs. PN10A represents the first complete sequence of a PVY strain variant of the class reported as PVY(N)-W (or PVY(N:O)) type B. Specific PCR assays for two unique regions of NE-11 are presented that will allow the identification of this strain variant by other researchers.
authors.affiliation: International Institute of Tropical Agriculture, Kampala, Uganda. jiml@uidaho.edu
authors.forename: Jim
authors.initials: J
authors.lastname: Lorenzen
authors.forename: Phil
authors.initials: P
authors.lastname: Nolte
authors.forename: Darren
authors.initials: D
authors.lastname: Martin
authors.forename: Julie S
authors.initials: JS
authors.lastname: Pasche
authors.forename: Neil C
authors.initials: NC
authors.lastname: Gudmestad
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011995:Recombination, Genetic