title: A Study of the interaction of HEK-293 cells with streamline chelating adsorbent in expanded bed operation.
abstract: Expanded bed adsorption (EBA) is an efficient protein purification process reducing time and steps of downstream processing (DSP) since nonclarified culture media can be processed directly without prior treatments such as filtration or centrifugation. However, cells and debris can interact with the adsorbent and affect bed stability as well as purification performance. To optimize EBA operating conditions these biomass/adsorbent interactions have to be understood and characterized. The adsorption of Human Embryonic Kidney cells (HEK 293) on unprimed and nickel-primed metal affinity adsorbent was studied in a closed loop EBA setup. With the unprimed adsorbent, the overall level of interaction observed was nonsignificant. With the nickel-primed adsorbent and an initial cell concentration ranging from 0.08 x 10(6) to 0.2 x 10(6) cells/mL, biomass/adsorbent interaction was found to be moderate and the adsorption apparent first-order kinetic rate constant was determined to be k = 0.009 to 0.011 min(-1).
authors.affiliation: Biotechnology and pharmaceutical processes research unit (Bio-P2), Department of Chemical Engineering, Ecole Polytechnique, Montréal, Québec, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Poulin
authors.forename: Renaud
authors.initials: R
authors.lastname: Jacquemart
authors.forename: Gregory
authors.initials: G
authors.lastname: De Crescenzo
authors.forename: Mario
authors.initials: M
authors.lastname: Jolicoeur
authors.forename: Robert
authors.initials: R
authors.lastname: Legros
mesh_terms: D000327:Adsorption; D018533:Biomass; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D002614:Chelating Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation*; D006801:Humans; D009532:Nickel / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D009532:Nickel