ATRA-inhibited proliferation in glioma cells is associated with subcellular redistribution of beta-catenin via up-regulation of Axin.
Retinoic acid (RA) is a major chemopreventive agent which exerts strong anti-tumor activity partly by trans-repressing the Wnt/beta-catenin signaling pathway in some tumor cell lines. However, the definite mechanism of RA trans-repression of the Wnt/beta-catenin signaling pathway has not been elucidated clearly. In this work, we found that all-trans retinoic acid (ATRA) significantly inhibited proliferation of glioma cells, accompanied by up-regulation of expression of Axin and altered subcellular distribution of beta-catenin. Transfecting C6 cells with rAxin further confirmed that increased expression of Axin is obligate for inhibition of proliferation and the increase of the cytoplasmic beta-catenin. Our results suggested that Axin might play an important role in RA-mediated anti-proliferative effects of glioma cell lines.
Lu
Jianrong
J
Department of Pathology, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, PR China.
Zhang
Feng
F
Zhao
Daqing
D
Hong
Liu
L
Min
Jie
J
Zhang
Liying
L
Li
Fanfan
F
Yan
Yan
Y
Li
Hang
H
Ma
Yu
Y
Li
Qing
Q
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D060466:Axin Protein; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D021381:Protein Transport; D051381:Rats; D012097:Repressor Proteins / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D015854:Up-Regulation; D051176:beta Catenin / Q000187:drug effects / Q000378:metabolism*
D000970:Antineoplastic Agents; D060466:Axin Protein; D012097:Repressor Proteins; D051176:beta Catenin; D014212:Tretinoin