Cell surface Lactobacillus plantarum LA 318 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) adheres to human colonic mucin.
OBJECTIVE
To characterize the adhesion molecule of Lactobacillus plantarum LA 318 that shows high adhesion to human colonic mucin (HCM).


RESULTS
The adhesion test used the BIACORE assay where PBS-washed bacterial cells showed a significant decrease in adherence to HCM than distilled water-washed cells. A component in the PBS wash fraction adhered to the HCM and a main protein was detected as a c. 40-kDa band using SDS-PAGE. Using homology comparisons of the N-terminal amino acid sequences compared with sequence databases, this protein was identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The DNA sequence of LA 318 GAPDH was 100% identical to the GAPDH (gapB) of L. plantarum WCFS1. The purified GAPDH adhered to HCM.


CONCLUSIONS
We found the adhesin of L. plantarum LA 318 to HCM in its culture PBS wash fraction. The molecule was identified as GAPDH. Because LA 318 possesses the same adhesin as many pathogens, the lactobacilli GAPDH may compete with pathogens infecting the intestine.


CONCLUSIONS
This is the first report showing GAPDH expressed on the cell surface of lactobacilli adheres to mucin suggesting L. plantarum LA 318 adheres to HCM using GAPDH binding activity to colonize the human intestinal mucosa.
Kinoshita
H
H
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi, Japan.
Uchida
H
H
Kawai
Y
Y
Kawasaki
T
T
Wakahara
N
N
Matsuo
H
H
Watanabe
M
M
Kitazawa
H
H
Ohnuma
S
S
Miura
K
K
Horii
A
A
Saito
T
T
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D001483:Base Sequence; D003106:Colon / Q000378:metabolism / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000502:physiology; D008969:Molecular Sequence Data; D009077:Mucins / Q000302:isolation & purification / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D019936:Probiotics; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D009077:Mucins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases