[Hyperplasia suppressor gene expression in vascular smooth muscle cells derived from normotensive and hypertensive patients underwent bypass surgery].
OBJECTIVE
To investigate the novel hyperplasia suppressor gene (HSG) expression in vascular smooth muscle cells derived from normotensive and hypertensive patients underwent bypass surgery.


METHODS
Coronary heart disease patients underwent coronary artery bypass graft (CABG) operation in BEIJING ANZHEN hospital from 4 - 9, 2006 were enrolled in this study and divided into hypertensive group (n = 28) and normotensive group (n = 26). The preoperative venous blood samples were taken for serum biochemical and vasoactive peptides measurements. Total RNA was extracted from WBC, explanted-vessels and cultured VSMCs using TRIZOL and HSG expression was determined by Semi-Quantitative RT-PCR.


RESULTS
Body mass index (BMI) was significantly higher in hypertensive group compared to normotensive group (P < 0.01) while other biochemic parameters and vasoactive peptides were similar between the groups. BMI and GLU, BMI and SBP, BMI and DBP, GLU and TG, SBP and DBP were positively correlated (all P < 0.05). HSG expression in WBC, VSMCs and vessel tissue were significantly lower in hypertensive group than those in normotensive group (all P < 0.05). HSG expression in tissue was negatively correlated to BMI, SBP and DBP (all P < 0.05).


CONCLUSIONS
Reduced HSG expression and the negative correlation on vascular tissue HSG expression to BMI, SBP and DBP suggested a possible inhibitory role of HSG on VSMC proliferation and blood pressure.
Liu
Ya-ping
YP
Hypertension Research Lab, Beijing Institute of Heart, Lung, Vascular Disease, Beijing Anzhen Hospital, Capital University of Medical Science, Beijing 100029, China.
Wen
Shao-jun
SJ
Liu
Ya
Y
Zhao
Li-min
LM
Guo
Yan-hong
YH
Chen
Xin-jun
XJ
Wang
Zuo-guang
ZG
Liu
Jie-lin
JL
Wen
Jie
J
Wang
Shi-Qi
SQ
Tang
Jian
J
D000368:Aged; D015992:Body Mass Index; D005260:Female; D015870:Gene Expression; D016153:Genes, Suppressor; D006801:Humans; D006965:Hyperplasia / Q000235:genetics; D006973:Hypertension / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*