title: Glucose-permeable interpenetrating polymer network hydrogels for corneal implant applications: a pilot study.
abstract: Epithelialization of a keratoprosthesis requires that the implant material be sufficiently permeable to glucose. We have developed a poly(ethylene glycol)/poly(acrylic acid) (PEG/PAA) interpenetrating polymer network (IPN) hydrogel that can provide adequate passage of glucose from the aqueous humor to the epithelium in vivo. A series of PEG/PAA IPNs with varying PEG macromonomer molecular weights were synthesized and evaluated through swelling studies to determine their water content and diffusion experiments to assess their permeability to glucose. One of the PEG/PAA hydrogels prepared in this study had a glucose diffusion coefficient nearly identical to that of the human cornea (approximately 2.5 x 10(-6) cm(2)/sec). When implanted intrastromally in rabbit corneas, this hydrogel was retained and well-tolerated in 9 out of 10 cases for a period of 14 days. The retained hydrogels stayed optically clear and the epithelium remained intact and multilayered, indicating that the material facilitated glucose transport from the aqueous humor to the anterior part of the eye. The results from these experiments indicate that PEG/PAA hydrogels are promising candidates for corneal implant applications such as keratoprostheses and intracorneal lenses, and that the PEG/PAA IPN system in general is useful for creating permeable substrates for ophthalmic and other biomedical applications.
authors.affiliation: Department of Ophthalmology, Stanford University, Stanford, California 94305, USA.
authors.forename: David
authors.initials: D
authors.lastname: Myung
authors.forename: Nabeel
authors.initials: N
authors.lastname: Farooqui
authors.forename: Dale
authors.initials: D
authors.lastname: Waters
authors.forename: Spencer
authors.initials: S
authors.lastname: Schaber
authors.forename: Wongun
authors.initials: W
authors.lastname: Koh
authors.forename: Michael
authors.initials: M
authors.lastname: Carrasco
authors.forename: Jaan
authors.initials: J
authors.lastname: Noolandi
authors.forename: Curtis W
authors.initials: CW
authors.lastname: Frank
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Ta
mesh_terms: D000818:Animals; D001187:Artificial Organs; D003319:Corneal Stroma / Q000601:surgery*; D005947:Glucose / Q000378:metabolism*; D020100:Hydrogels / Q000378:metabolism*; D010539:Permeability; D010865:Pilot Projects; D019736:Prostheses and Implants; D019919:Prosthesis Implantation; D011817:Rabbits
chemical_list: D020100:Hydrogels; D005947:Glucose