title: siRNA-mediated inhibition of phosphatidylinositol glycan Class B (PIGB) confers resistance to methylmercury in HEK293 cells.
abstract: We performed functional gene screening, using a siRNA library targeting 8,500 human genes, to identify proteins that are involved in the susceptibility of cells to methylmercury. Screening revealed that downregulation of the gene for phosphatidylinositol glycan class B (PIGB) by siRNA confers resistance to methylmercury in HEK293 cells.
authors.affiliation: Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors.forename: Gi-Wook
authors.initials: GW
authors.lastname: Hwang
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Kayoko
authors.initials: K
authors.lastname: Kita
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Shusuke
authors.initials: S
authors.lastname: Kuge
authors.forename: Akira
authors.initials: A
authors.lastname: Naganuma
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance / Q000235:genetics*; D006801:Humans; D008364:Mannosyltransferases / Q000235:genetics* / Q000378:metabolism; D008767:Methylmercury Compounds / Q000633:toxicity*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection
chemical_list: D008767:Methylmercury Compounds; D034741:RNA, Small Interfering; D008364:Mannosyltransferases; C102168:PIGB protein, human; C004925:methylmercuric chloride