beta-Actin-derived peptides isolated from acidic extract of rat spleen suppress tumor cell growth.
Twenty-two fragments of beta-actin and beta-actin-related protein were isolated from the acidic extracts of rat spleen tissue. beta-Actin fragments (75-90), (78-89), and (78-88), 0.01-1 microM, decreased live cell number of L929 murine tumor fibroblasts by 80-90%, with maximal cytotoxic effect of 30-40%. The fragments of (78-90) segment and the fragment of beta-actin-related protein (69-77) were less active (inhibitory effect up to 55%, cytotoxic-up to 25%).
Blishchenko
Elena Y
EY
Shemyakin-Ovchinnikov Institute of Bio-organic Chemistry, Russian Academy of Sciences, Moscow, V-437, GSP, Russia.
Sazonova
Olga V
OV
Yatskin
Oleg N
ON
Kalinina
Olga A
OA
Tolmazova
Anna G
AG
Philippova
Marina M
MM
Karelin
Andrei A
AA
Ivanov
Vadim T
VT
D000143:Acids / Q000737:chemistry*; D000199:Actins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000473:pathology*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry
D000143:Acids; D000199:Actins; D010455:Peptides; D014020:Tissue Extracts