Expression and characterization of VP2 protein of human rotavirus a in a mammalian lung cell line.
A system for the expression and characterization of VP2 protein of Human rotavirus A, strain G3 was established in the mammalian lung cell line (A549). Expression of the recombinant VP2 (rVP2) protein was detected 48-72 hrs after transfection by Western blotting. The rVP2 protein expressed in A549 cells formed intracellular core-like particles (CLPs) 30 nm in diameter detected by electron microscopy (EM). These results showed that the A549 cells are suitable as efficient eukaryotic host for production of rVP2 protein.
Pourasgari
F
F
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. pourasgari@ibb.ut.ac.ir
Ahmadian
S
S
Salmanian
A H
AH
D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism*; D002460:Cell Line; D003600:Cytosol / Q000821:virology; D004847:Epithelial Cells / Q000821:virology*; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000166:cytology / Q000821:virology; D012401:Rotavirus / Q000235:genetics*; D022701:Virosomes / Q000648:ultrastructure
D036022:Capsid Proteins; C442545:VP2 protein, Rotavirus; D022701:Virosomes