Simultaneous detection of IFN-gamma and IL-4 in lesional tissues and whole unstimulated saliva from patients with oral lichen planus.
BACKGROUND
Although the roles of interferon (IFN)-gamma and interleukin (IL)-4 in oral lichen planus (OLP) have been described extensively in past decades, the available results are controversial. Moreover, few studies have utilized simultaneous detection of cytokines in local tissues and saliva to determine whether salivary cytokines could reflect the fact of local lesions.


METHODS
IFN-gamma and IL-4 were determined simultaneously in lesions and whole unstimulated saliva (WUS) from OLP patients with various clinical forms.


RESULTS
In OLP lesions, both IFN-gamma and IL-4 in erythematous/ulcerated OLP were higher significantly than that in control specimens. In WUS, however, only IFN-gamma of erythematous/ulcerated OLP was increased compared with control. Remarkably, IFN-gamma and IL-4 in WUS showed a more significant correlation to those in local tissues of all subjects.


CONCLUSIONS
These results indicate that both IFN-gamma and IL-4 may play more important role in pathogenesis of erythematous/ulcerated OLP, and changes of these proinflammatory cytokines in WUS may reflect the status of the OLP lesion.
Tao
Xiao-an
XA
Department of Oral Medicine, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
Li
Chun-yang
CY
Rhodus
Nelson L
NL
Xia
Juan
J
Yang
Xiao-ping
XP
Cheng
Bin
B
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D008010:Lichen Planus / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D012463:Saliva / Q000737:chemistry; D018709:Statistics, Nonparametric
D015847:Interleukin-4; D007371:Interferon-gamma