Isolation by HPLC and characterisation of the bioactive fraction of New Zealand manuka (Leptospermum scoparium) honey.
Using HPLC a fraction of New Zealand manuka honey has been isolated, which gives rise to the non-peroxide antibacterial activity. This fraction proved to be methylglyoxal, a highly reactive precursor in the formation of advanced glycation endproducts (AGEs). Methylglyoxal concentrations in 49 manuka and 34 non-manuka honey samples were determined using a direct detection method and compared with values obtained using standard o-phenylenediamine derivatisation. Concentrations obtained using both the methods were similar and varied from 38 to 828 mg/kg.
Adams
Christopher J
CJ
Chemistry Department, University of Waikato, Private Bag 3105, Hamilton, New Zealand.
Boult
Cherie H
CH
Deadman
Benjamin J
BJ
Farr
Judie M
JM
Grainger
Megan N C
MN
Manley-Harris
Merilyn
M
Snow
Melanie J
MJ
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D006722:Honey / Q000032:analysis*; D031649:Leptospermum / Q000737:chemistry*; D050296:Microbial Viability / Q000187:drug effects; D010545:Peroxides / Q000737:chemistry; D011765:Pyruvaldehyde / Q000737:chemistry
D000900:Anti-Bacterial Agents; D010545:Peroxides; D011765:Pyruvaldehyde