title: Butyrate enemas upregulate Muc genes expression but decrease adherent mucus thickness in mice colon.
abstract: Colonic mucosal protection is provided by the mucus gel, mainly composed of mucins. Several factors can modulate the formation and the secretion of mucins, and among them butyrate, an end-product of carbohydrate fermentation. However, the specific effect of butyrate on the various colonic mucins, and the consequences in terms of the mucus layer thickness are not known. Our aim was to determine whether butyrate modulates colonic MUC genes expression in vivo and whether this results in changes in mucus synthesis and mucus layer thickness. Mice received daily for 7 days rectal enemas of butyrate (100 mM) versus saline. We demonstrated that butyrate stimulated the gene expression of both secreted (Muc2) and membrane-linked (Muc1, Muc3, Muc4) mucins. Butyrate especially induced a 6-fold increase in Muc2 gene expression in proximal colon. However, butyrate enemas did not modify the number of epithelial cells containing the protein Muc2, and caused a 2-fold decrease in the thickness of adherent mucus layer. Further studies should help understanding whether this last phenomenon, i.e. the decrease in adherent mucus gel thickness, results in a diminished protective function or not.
authors.affiliation: UMR - PhAN & CRNH, Physiologie des Adaptations Nutritionnelles, Nantes Cedex 1, France.
authors.forename: E
authors.initials: E
authors.lastname: Gaudier
authors.forename: M
authors.initials: M
authors.lastname: Rival
authors.forename: M-P
authors.initials: MP
authors.lastname: Buisine
authors.forename: I
authors.initials: I
authors.lastname: Robineau
authors.forename: C
authors.initials: C
authors.lastname: Hoebler
mesh_terms: D000268:Adhesiveness; D000285:Administration, Rectal; D000818:Animals; D002087:Butyrates / Q000008:administration & dosage*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D004733:Enema; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018396:Mucin-1 / Q000378:metabolism; D055262:Mucin-2 / Q000378:metabolism; D055263:Mucin-3 / Q000378:metabolism; D055264:Mucin-4 / Q000378:metabolism; D009077:Mucins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D002087:Butyrates; C524626:Muc2 protein, mouse; C524641:Muc3 protein, mouse; C524659:Muc4 protein, mouse; D018396:Mucin-1; D055262:Mucin-2; D055263:Mucin-3; D055264:Mucin-4; D009077:Mucins