title: Heterogeneity of lymphoid tissue inducer cell populations present in embryonic and adult mouse lymphoid tissues.
abstract: Lymphoid tissue inducer (LTi) cells have a well established role in secondary lymphoid tissue development. Here, we report on the heterogeneity of LTi cells based on their CD4 and chemokine receptor expression. The CD4(-) LTi-cell population has a similar phenotype to the CD4(+) population, with similar chemokine-receptor-expressing subsets. In both embryonic and adult spleen the CD4(-) LTi-cell population is comparable as a proportion of total splenocytes to its CD4(+) counterpart. In contrast, different proportions of CD4(+) and CD4(-) LTi cells are found in different lymph nodes. Both CD4(+) and CD4(-) LTi cells share the anatomical location and are associated with vascular cell adhesion molecule-1-positive stromal cells in spleen and lymph nodes. The numbers of both CD4(+) and CD4(-) LTi cells in adult spleen are augmented in the presence of B cells. With the exception of CD4, there is a strong correlation coefficient (0.89) for gene expression between the two populations. Polymerase chain reaction analysis of individual CD4(+) and CD4(-) LTi cells shows that a similar proportion in embryonic and adult spleen co-expressed both CXCR5 and CCR7 or CXCR5 alone: 84.6% for adult CD4(+) and 87.6% for adult CD4(-); 95.3% for embryonic CD4(+) and 91.5% for embryonic CD4(-). Consistently fewer CCR7 single-positive cells were found in the CD4(+) and CD4(-) fractions in the embryo.
authors.affiliation: MRC Centre for Immune Regulation, Institute for Biomedical Research, Birmingham Medical School, Birmingham, UK. kimmy@ssu.ac.kr
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
authors.forename: Simona
authors.initials: S
authors.lastname: Rossi
authors.forename: David
authors.initials: D
authors.lastname: Withers
authors.forename: Fiona
authors.initials: F
authors.lastname: McConnell
authors.forename: Kai-Michael
authors.initials: KM
authors.lastname: Toellner
authors.forename: Fabrina
authors.initials: F
authors.lastname: Gaspal
authors.forename: Eric
authors.initials: E
authors.lastname: Jenkinson
authors.forename: Graham
authors.initials: G
authors.lastname: Anderson
authors.forename: Peter J L
authors.initials: PJ
authors.lastname: Lane
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D004622:Embryo, Mammalian / Q000276:immunology*; D015870:Gene Expression / Q000276:immunology; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction / Q000379:methods; D054400:Receptors, CCR7 / Q000378:metabolism; D054380:Receptors, CXCR5 / Q000378:metabolism; D013154:Spleen / Q000196:embryology / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: C513179:CXCR5 protein, mouse; C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7; D054380:Receptors, CXCR5; D019010:Vascular Cell Adhesion Molecule-1