title: High prevalence of human Torque teno virus in streams crossing the city of Manaus, Brazilian Amazon.
abstract: OBJECTIVE
  Torque teno virus (TTV) is a human DNA virus chronically infecting most healthy individuals worldwide and can be transmitted by faecal-oral route. The occurrence of TTV was evaluated in the streams crossing the city of Manaus (Brazilian Amazon) over a 1-year period, four times a year.
  
  
  RESULTS
  Fifty-two water samples were collected from 13 different locations. Viruses were concentrated from two litres of water by adsorption to negative membrane filters followed by ultrafiltration. TTV DNA was detected by PCR assays designed to detect all five TTV genomic groups. By conventional PCR, 19/52 (37%) samples were positive. By real-time PCR, TTV DNA could be detected in 48/52 (92%) samples. Viral loads ranged from 1300 to 746 000 genome equivalent per 100 ml of river water. Eleven distinct nucleotide sequences were obtained.
  
  
  CONCLUSIONS
  Our results show the wide distribution and diversity of TTV among Manaus urban micro basins.
  
  
  CONCLUSIONS
  The data presented here may contribute to substantiate TTV as a sensitive indicator of human contamination.
authors.affiliation: Laboratory of Molecular Virology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Diniz-Mendes
authors.forename: V S de
authors.initials: VS
authors.lastname: Paula
authors.forename: S L B
authors.initials: SL
authors.lastname: Luz
authors.forename: C
authors.initials: C
authors.lastname: Niel
mesh_terms: D001483:Base Sequence; D001938:Brazil; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D045483:Rivers; D022783:Torque teno virus / Q000235:genetics*; D014504:Urban Health; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D017931:DNA Primers; D004279:DNA, Viral