[Inhibitory effects of Qushi Huayu Decoction on fatty deposition and tumor necrosis factor alpha secretion in HepG2 cells induced by free fatty acid].
OBJECTIVE
To study the effects of Qushi Huayu Decoction (QHD) contained serum on fatty deposition and tumor necrosis factor alpha (TNF-alpha) secretion of hepatic lipotoxicity model in vitro, for further investigating the mechanism of the decoction for preventing and treating fatty liver.


METHODS
The steatosis with TNF-alpha secretion lipotoxic model of HepG2 induced by long-chain free fatty acid (FFA) was duplicated. Groups of normal, model cells and model cells treated with different concentrations of QHD contained serum were set up to test the content of TNF-alpha in culture supernate and triglyceride (TG) in cells, as well as to observe the ultrastructural change of cells by oil-red staining and the protein expression and gene expression of cellular TNF-alpha.


RESULTS
After being stimulated with FFA for 24 h, marked deposition of lipid with high content of TG presented in the cells of model group. Compared with the normal group, not only TNF-alpha content of culture supernate but also the protein expression and mRNA expression of intracellular TNF-alpha increased significantly. Contrast to the model group, the contents of TG in cells and TNF-alpha in supernate as well as the protein and mRNA expression of TNF-alpha in the model cell group treated with 10% QHD were lower significantly (all P < 0.01).


CONCLUSIONS
QHD could significantly inhibit the fatty deposition and TNF-alpha secretion in HepG2 cells induced by free fatty acid.
Zhang
Hui
H
Shuguang Hospital Affiliated to Shanghai University of TCM, Institute of Liver Disease of Shanghai University of TCM, Shanghai.
Hu
Yi-Yang
YY
Feng
Qin
Q
D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D018197:Hepatoblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D046529:Microscopy, Electron, Transmission; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum; D014280:Triglycerides / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D012333:RNA, Messenger; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; C533465:qushi huayu