Ex vivo magnetofection with magnetic nanoparticles: a novel platform for nonviral tissue engineering.
Several methods have been described to introduce DNA expression vectors into mammalian cells both in vitro and in vivo. Each system has benefits and limitations, and to date there is still no ideal method for gene transfer. In this study, we introduced a novel method of gene transfer by using Fe3O4 nanoparticles. The magnetic nanoparticles composed of Fe3O4, and the transfected genes used are Lac Z and enhanced green fluorescence protein gene (EGFG). Four different groups of preparations included in this study were homemade liposome-enveloped EGFP-DNA/Fe3O4, homemade liposome EGFP-DNA gene without magnetic Fe3O4 nanoparticles, lipofectamine 2000-enveloped EGFP-DNA, and EGFP-DNA gene only. Mice osteoblast and He99 lung cancer cell line were used as host cells for gene transfection. The time-dependent EGFP gene expression was monitored and analyzed. The results showed that the diameter of the complex was less than 100 nm. There was no cytotoxicity observed at any of the magnetic Fe3O4 nanoparticle concentrations tested. In the presence of magnetic field, the liposome-enveloped EGFP-DNA/Fe3O4 complex exhibited a much higher efficiency for transfecting EGFP-DNA into osteoblast cells under external magnetic fields. The gene can be transfected into cells with an aid of magnetic vectors and magnetic force. Under a gradient magnetic field, the efficiency of magnetofection is enhanced as compared to that without magnetic field.
Yang
Shieh-Yueh
SY
Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, Taiwan.
Sun
Jui-Sheng
JS
Liu
Cheng-Heng
CH
Tsuang
Yang-Hwei
YH
Chen
Li-Ting
LT
Hong
Chin-Yih
CY
Yang
Hong-Chang
HC
Horng
Herng-Er
HE
D000818:Animals; D000831:Animals, Newborn; D045744:Cell Line, Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004705:Endocytosis; D052203:Ferrosoferric Oxide / Q000737:chemistry* / Q000633:toxicity; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008081:Liposomes; D008175:Lung Neoplasms / Q000378:metabolism; D008280:Magnetics; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D023822:Tissue Engineering; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D004247:DNA; D001616:beta-Galactosidase; D052203:Ferrosoferric Oxide