title: beta-Actin-derived peptides isolated from acidic extract of rat spleen suppress tumor cell growth.
abstract: Twenty-two fragments of beta-actin and beta-actin-related protein were isolated from the acidic extracts of rat spleen tissue. beta-Actin fragments (75-90), (78-89), and (78-88), 0.01-1 microM, decreased live cell number of L929 murine tumor fibroblasts by 80-90%, with maximal cytotoxic effect of 30-40%. The fragments of (78-90) segment and the fragment of beta-actin-related protein (69-77) were less active (inhibitory effect up to 55%, cytotoxic-up to 25%).
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bio-organic Chemistry, Russian Academy of Sciences, Moscow, V-437, GSP, Russia.
authors.forename: Elena Y
authors.initials: EY
authors.lastname: Blishchenko
authors.forename: Olga V
authors.initials: OV
authors.lastname: Sazonova
authors.forename: Oleg N
authors.initials: ON
authors.lastname: Yatskin
authors.forename: Olga A
authors.initials: OA
authors.lastname: Kalinina
authors.forename: Anna G
authors.initials: AG
authors.lastname: Tolmazova
authors.forename: Marina M
authors.initials: MM
authors.lastname: Philippova
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Karelin
authors.forename: Vadim T
authors.initials: VT
authors.lastname: Ivanov
mesh_terms: D000143:Acids / Q000737:chemistry*; D000199:Actins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000473:pathology*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000378:metabolism*; D014020:Tissue Extracts / Q000737:chemistry
chemical_list: D000143:Acids; D000199:Actins; D010455:Peptides; D014020:Tissue Extracts