title: Mitosis orientation in prostate epithelial cells changed by endocrine effect.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effect of androgen and estrogen on mitosis orientation in the prostate epithelial cells of male rats.
  
  
  METHODS
  Castrated rats were treated with a single injection of testosterone propionate (TP) or benzogynestry (E2). There were 8 rats in the control group and TP-treated or E2-treated group. Prostate, liver, a specimen of skin, and a segment of the jejunum and colon were removed after the corresponding treatment. The results were observed through immunohistochemistry and iron hematoxylin-eosin staining.
  
  
  RESULTS
  All mitoses found in the prostate epithelial cells of castrated rats with TP were oriented parallel to the basement membrane; however, mitoses found in the prostate epithelial cells of castrated rats in E2 and the control group were oriented perpendicular to the basement membrane. TP treatment resulted in marked changes in mitosis orientation in the prostate epithelial cells. Bromodeoxyuridine-labeled positive cells could be seen throughout the stroma and prostate epithelial cells with an injection of TP; however, the positive cells could only be seen in the stroma of prostate with an injection of E2, and the positive cells could hardly be seen in the control group.
  
  
  CONCLUSIONS
  We found a novel effect of TP in the prostate as a marked change of mitosis orientation in prostate epithelial cells.
authors.affiliation: Fudan University, Shanghai 200032, China.
authors.forename: Xiang-yun
authors.initials: XY
authors.lastname: Liu
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Li
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Zhang
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Wu
authors.forename: Zu-yue
authors.initials: ZY
authors.lastname: Sun
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004702:Endocrine Glands / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008938:Mitosis / Q000502:physiology*; D011467:Prostate / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043343:Testosterone Propionate / Q000494:pharmacology
chemical_list: D043343:Testosterone Propionate