title: Expression and purification of CB2 for NMR studies in micellar solution.
abstract: We demonstrate feasibility of biophysical characterization of the peripheral cannabinoid receptor CB2 produced by heterologous expression in E. coli membranes. Recombinant receptor was purified by affinity chromatography, and NMR diffusion experiments performed on CB2 solubilized in dodecylphosphocholine (DPC) micelles. Circular dichroism spectroscopy indicated high alpha-helical content (49 %) of CB2.
authors.affiliation: National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health, Bethesda, MD 20892-9410, USA.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Krepkiy
authors.forename: Klaus
authors.initials: K
authors.lastname: Gawrisch
authors.forename: Alexei
authors.initials: A
authors.lastname: Yeliseev
mesh_terms: D002186:Cannabinoids / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics; D002942:Circular Dichroism; D003902:Detergents / Q000737:chemistry; D004058:Diffusion; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D058619:Maltose-Binding Proteins; D008823:Micelles; D043885:Receptor, Cannabinoid, CB2 / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012996:Solutions
chemical_list: D002186:Cannabinoids; D002352:Carrier Proteins; D003902:Detergents; D044385:GTP-Binding Protein alpha Subunits; D008024:Ligands; D058619:Maltose-Binding Proteins; D008823:Micelles; D043885:Receptor, Cannabinoid, CB2; D011993:Recombinant Fusion Proteins; D012996:Solutions