Detection of a gamma-carboxy-glutamate as novel post-translational modification of human transthyretin.
During analysis of the proteome in the cerebrospinal fluid (CSF) of the Caucasian form of moyamoya disease (MMD), a novel post-translational modification of human transthyretin was observed. Two-dimensional electrophoresis and subsequent peptide sequencing with ESI-MS/MS were performed to discover the gamma-carboxylation of the Glu-42 (Gla-42).
Rüggeberg
Sabrina
S
European Molecular Biology Laboratory, Proteomic Core Facility, Meyerhofstr. 1. 69117 Heidelberg, Germany.
Horn
Peter
P
Li
Xinping
X
Vajkoczy
Peter
P
Franz
Thomas
T
D015055:1-Carboxyglutamic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D000328:Adult; D001665:Binding Sites; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009072:Moyamoya Disease / Q000134:cerebrospinal fluid / Q000378:metabolism*; D011228:Prealbumin / Q000134:cerebrospinal fluid / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
D011228:Prealbumin; D020543:Proteome; D015055:1-Carboxyglutamic Acid