title: Single nucleotide polymorphisms of the MCHR1 gene do not affect metabolism in humans.
abstract: Melanin concentrating hormone receptor-1 (MCHR1) is a centrally and peripherally expressed receptor that regulates energy expenditure and appetite. Single nucleotide polymorphisms (SNPs) of the MCHR1 gene have been previously associated with obesity, but the results are inconsistent among different populations. This study was performed to determine whether SNPs of MCHR1 affect glucose and energy metabolism. We screened six SNPs of MCHR1 in a cross-sectional study of 217 middle-age, non-diabetic Finnish subjects who were offspring of type 2 diabetic patients. Insulin secretion was evaluated by an intravenous glucose tolerance test and insulin sensitivity and energy metabolism by the hyperinsulinemic euglycemic clamp and indirect calorimetry. SNPs of MCHR1 were not associated with BMI, waist circumference, subcutaneous or intra-abdominal fat area, glucose tolerance, first-phase insulin release, insulin sensitivity, or energy metabolism. One SNP, which was in >0.50 linkage disequilibrium with the other five SNPs, was also screened in 1455 unrelated Finnish middle-age subjects in a population-based study. No differences in BMI, waist circumference, or glucose or insulin levels in an oral glucose tolerance test among the genotypes were found. In conclusion, SNPs of MCHR1 did not have effects on metabolic variables in humans.
authors.affiliation: Department of Medicine, University of Kuopio and Kuopio University Hospital, 70210 Kuopio, Finland.
authors.forename: Jarno
authors.initials: J
authors.lastname: Rutanen
authors.forename: Jussi
authors.initials: J
authors.lastname: Pihlajamäki
authors.forename: Markku
authors.initials: M
authors.lastname: Vänttinen
authors.forename: Urpu
authors.initials: U
authors.lastname: Salmenniemi
authors.forename: Eija
authors.initials: E
authors.lastname: Ruotsalainen
authors.forename: Teemu
authors.initials: T
authors.lastname: Kuulasmaa
authors.forename: Sakari
authors.initials: S
authors.lastname: Kainulainen
authors.forename: Johanna
authors.initials: J
authors.lastname: Kuusisto
authors.forename: Markku
authors.initials: M
authors.lastname: Laakso
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2; D004734:Energy Metabolism / Q000235:genetics*; D005387:Finland; D005951:Glucose Tolerance Test; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017481:Receptors, Somatostatin / Q000235:genetics*
chemical_list: C103263:MCHR1 protein, human; D017481:Receptors, Somatostatin