Enhanced production of SCL-LCL-PHA co-polymer by sludge-isolated Pseudomonas aeruginosa MTCC 7925.
OBJECTIVE
To boost short-chain-length-long-chain-length polyhydroxyalkanoate (SCL-LCL-PHA) co-polymer yield in Pseudomonas aeruginosa MTCC 7925 by manipulating culture conditions.


RESULTS
Stationary phase culture of P. aeruginosa MTCC 7925 was subjected to various doses of different carbons and, N and P deficiencies. Enhanced co-polymer yield was recorded under ethanol- and glucose-supplemented cultures. Interaction of ethanol with N-deficiency boosted co-polymer accumulation maximally.


CONCLUSIONS
The sludge-isolated P. aeruginosa MTCC 7925 demonstrated good capability to synthesize SCL-LCL-PHA co-polymer from unrelated carbon sources. Supplementation of ethanol under N-deficiency boosted the co-polymer yield up to 69% of dry cell weight, which is significantly higher when compared with other SCL-MCL-PHA co-polymer accumulating bacterial species.


CONCLUSIONS
This is the first report on P. aeruginosa MTCC 7925, which is capable of accumulating LCL 3-hydroxyhexadecanoate and 3-hydroxyoctadecanoate units with SCL 3-hydroxybutyrate and 3-hydroxyvalerate as constituents of PHAs. This opens up new possibilities for various industrial applications owing to the superior properties of this new co-polymer.
Singh
A K
AK
Agricultural and Food Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal, India.
Mallick
N
N
D001709:Biotechnology / Q000379:methods; D003470:Culture Media; D003651:Decanoates / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose; D006885:Hydroxybutyrates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000172:deficiency; D010421:Pentanoic Acids / Q000378:metabolism; D010758:Phosphorus / Q000172:deficiency; D011091:Polyesters / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*
D003470:Culture Media; D003651:Decanoates; D006885:Hydroxybutyrates; D010421:Pentanoic Acids; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D011108:Polymers; D012722:Sewage; D010758:Phosphorus; D000431:Ethanol; C038780:n-pentanoic acid; D005947:Glucose; D009584:Nitrogen