title: Alveolar macrophages from allergic lungs are not committed to a pro-allergic response and can reduce airway hyperresponsiveness following ex vivo culture.
abstract: BACKGROUND
  We already demonstrated that adoptive transfer of alveolar macrophages (AMs) from non-allergic rats into AM-depleted allergic rats prevents airway hyperresponsiveness (AHR). We also showed that AMs from non-sensitized, but not from sensitized, allergy-prone rats can prevent AHR following allergen challenge in sensitized allergic animals, establishing the importance of rat immunological status on the modulation of AM functions and suggesting that an allergic lung environment alters AM functions.
  
  
  OBJECTIVE
  We investigated how the activation of allergic AMs can be modulated to reinstitute them with their capacity to reduce AHR.
  
  
  METHODS
  AMs from sensitized Brown Norway rats were cultured ex vivo for up to 18 h in culture media to deprogram them from the influence of the allergic lung before being reintroduced into the lung of AM-depleted sensitized recipient. AHR and cytokines in bronchoalveolar lavage (BAL) were measured following allergen challenge. AMs stimulated ex vivo with Bacillus Calmette-Guerin (BCG) were used as positive controls as BCG induces a T-helper type 1 activation in AMs.
  
  
  RESULTS
  AMs ex vivo cultured for 4-18 h reduced AHR to normal level. Interestingly, pro-allergic functions of AMs were dampened by 18 h culture and they reduced AHR even after spending 48 h in an allergic lung microenvironment. Furthermore, transfer of cultured AMs caused an increase in the levels of IFN-gamma and IL-12 in BAL when compared with their ovalbumin control. After 18 h of ex vivo culture, AMs expressed reduced levels of TNF, IL-1alpha, IL-6, and Arginase-2 mRNAs compared with freshly isolated AMs, suggesting that ex vivo culture exempted AMs from lung stimuli that affected their functions.
  
  
  CONCLUSIONS
  There is a significant crosstalk between lung microenvironment and AMs, affecting their functions. It is also the first report showing that sensitized AMs can be modulated ex vivo to reduce lung pro-allergic environment, opening the way to therapies targetting AMs.
authors.affiliation: Centre de recherche de l'Hôpital Laval, Institut universitaire de cardiologie et de pneumologie de l'Université Laval, Québec, QC, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Pouliot
authors.forename: A
authors.initials: A
authors.lastname: Spahr
authors.forename: E
authors.initials: E
authors.lastname: Careau
authors.forename: V
authors.initials: V
authors.lastname: Turmel
authors.forename: E Y
authors.initials: EY
authors.lastname: Bissonnette
mesh_terms: D000818:Animals; D001119:Arginase / Q000235:genetics; D016535:Bronchial Hyperreactivity / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002469:Cell Separation; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008081:Liposomes / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011914:Rats, Inbred BN; D013997:Time Factors
chemical_list: D016207:Cytokines; D008081:Liposomes; D012333:RNA, Messenger; D010047:Ovalbumin; C113162:Arg2 protein, rat; D001119:Arginase