One step visual detection of PCR products with gold nanoparticles and a nucleic acid lateral flow (NALF) device.
Specific PCR products are detected with an antibody-free lateral-flow device by sandwiching them between reporter oligonucleotides covalently attached to gold nanoparticles (GNPs) and capture oligonucleotides covalently attached to a nitrocellulose chromatographic strip.
Aveyard
Jenny
J
Department of Chemistry, Liverpool University, Liverpool, L69 7ZD, UK.
Mehrabi
Maryam
M
Cossins
Andrew
A
Braven
Helen
H
Wilson
Robert
R
D003101:Collodion / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D011934:Reagent Strips; D012680:Sensitivity and Specificity
D009696:Nucleic Acids; D009841:Oligonucleotides; D011934:Reagent Strips; D006046:Gold; D003101:Collodion