Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions.
We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells.
Zhang
Kexiong
K
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06511, USA.
Wang
Qiaoqiao
Q
Xie
Yanhua
Y
Mor
Gil
G
Sega
Emanuela
E
Low
Philip S
PS
Huang
Yingqun
Y
D020029:Base Pairing; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D058973:Folate Receptors, GPI-Anchored; D020868:Gene Silencing; D015316:Genetic Therapy; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D039564:Integrin alphaV / Q000235:genetics; D008024:Ligands; D008869:Microtubule-Associated Proteins / Q000235:genetics; D009363:Neoplasm Proteins / Q000235:genetics; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D000077022:Survivin
C107489:BIRC5 protein, human; D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D051034:Inhibitor of Apoptosis Proteins; D039564:Integrin alphaV; D008024:Ligands; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D009696:Nucleic Acids; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D000077022:Survivin