Electrochemistry-mass spectrometry in drug metabolism and protein research.
The combination of electrochemistry coupled on-line to mass spectrometry (EC-MS) forms a powerful analytical technique with unique applications in the fields of drug metabolism and proteomics. In this review the latest developments are surveyed from both instrumental and application perspectives. The limitations and solutions for coupling an electrochemical system to a mass spectrometer are discussed. The electrochemical mimicking of drug metabolism, specifically by Cytochrome P450, is high-lighted as an application with high biomedical relevance. The EC-MS analysis of proteins also has promising new applications for both proteomics research and biomarker discovery. EC-MS has furthermore advantages for improved analyte detection with mass spectrometry, both for small molecules and large biomolecules. Finally, potential future directions of development of the technique are briefly discussed.
Permentier
Hjalmar P
HP
Mass Spectrometry Core Facility, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands. h.p.permentier@rug.nl
Bruins
Andries P
AP
Bischoff
Rainer
R
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D015195:Drug Design; D004563:Electrochemistry; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry*; D012107:Research Design
D004364:Pharmaceutical Preparations; D011506:Proteins; D003577:Cytochrome P-450 Enzyme System