Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor.
We report here the generation of transgenic chickens that produce human granulocyte-colony stimulating factor (hG-CSF) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector-encoded hG-GSF gene. The biological activity of the recombinant hG-CSF was significantly higher than its commercially derived E. coli-derived counterpart. Successful germline transmission of the transgene was also confirmed in G(1) transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G(1) progeny were dead within 1 month of hatching.
Kwon
Mo Sun
MS
Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea.
Koo
Bon Chul
BC
Choi
Bok Ryul
BR
Park
Yoon-Yub
YY
Lee
Young Man
YM
Suh
Hun Suk
HS
Park
Young Sik
YS
Lee
Hoon Taek
HT
Kim
Jin-Hoi
JH
Roh
Ji Yeol
JY
Kim
Nam-Hyung
NH
Kim
Teoan
T
D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques; D002642:Chick Embryo / Q000502:physiology; D002645:Chickens; D005260:Female; D005307:Fertilization in Vitro; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D010063:Ovum / Q000166:cytology / Q000502:physiology*; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics; D012661:Semen
D016179:Granulocyte Colony-Stimulating Factor