title: Isolation of lactic acid bacteria with inhibitory activity against pathogens and spoilage organisms associated with fresh meat.
abstract: The use of lactic acid bacteria (LAB) as protective cultures in vacuum-packed chill-stored meat has potential application for assuring and improving food quality, safety and market access. In a study to identify candidate strains suitable for evaluation in a meat model, agar-based methods were employed to screen 181 chilled meat and meat process-related LAB for strains inhibitory to pathogens and spoilage organisms of importance to the meat industry. Six meat-derived strains, including Lactobacillus sakei and Lactococcus lactis, were found to be inhibitory to one or more of the target strains Listeria monocytogenes, Brochothrix thermosphacta, Campylobacter jejuni and Clostridium estertheticum. The inhibitory agents appeared to be either cell-associated or molecules released extracellularly with bacteriocin-like properties. Variations detected in the antimicrobial activity of LAB associated with changes to test parameters such as substrate composition underlined the importance of further in situ evaluation of the inhibitory strains in stored meat trials.
authors.affiliation: Food, Metabolism and Microbiology, Agresearch, Private Bag 3123, Hamilton, New Zealand. rhys.jones@agresearch.co.nz
authors.forename: Rhys J
authors.initials: RJ
authors.lastname: Jones
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Hussein
authors.forename: Monique
authors.initials: M
authors.lastname: Zagorec
authors.forename: Gale
authors.initials: G
authors.lastname: Brightwell
authors.forename: John R
authors.initials: JR
authors.lastname: Tagg
mesh_terms: D000818:Animals; D000898:Antibiosis; D016123:Campylobacter jejuni / Q000254:growth & development; D003013:Clostridium / Q000254:growth & development; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005506:Food Contamination / Q000517:prevention & control*; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods; D005519:Food Preservation / Q000379:methods*; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification / Q000502:physiology*; D008089:Listeria monocytogenes / Q000254:growth & development; D008460:Meat / Q000382:microbiology*; D013997:Time Factors; D014618:Vacuum