title: High-resolution structure of methionine gamma-lyase from Citrobacter freundii.
abstract: Pyridoxal 5'-phosphate-dependent methionine gamma-lyase (MGL) is involved in the metabolism of sulfur-containing amino acids. The enzyme is a promising target in some anaerobic pathogens and is effective in cancer-cell treatment. The structure of the MGL holoenzyme from Citrobacter freundii has previously been determined at 1.9 A resolution. By modification of the crystallization procedure, the previously determined structure of C. freundii MGL has been improved to 1.35 A resolution with R and R(free) values of 0.152 and 0.177, respectively. This high-resolution structure makes it possible to analyze the interactions between the monomers in detail and to reveal the structurally invariant regions that are responsible for monomer-monomer recognition during the formation of the active enzyme. Details of the mode of cofactor binding and of the flexible regions that may be involved in substrate recognition and binding are also described.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
authors.forename: Alexei
authors.initials: A
authors.lastname: Nikulin
authors.forename: Svetlana
authors.initials: S
authors.lastname: Revtovich
authors.forename: Elena
authors.initials: E
authors.lastname: Morozova
authors.forename: Natalia
authors.initials: N
authors.lastname: Nevskaya
authors.forename: Stanislav
authors.initials: S
authors.lastname: Nikonov
authors.forename: Maria
authors.initials: M
authors.lastname: Garber
authors.forename: Tatyana
authors.initials: T
authors.lastname: Demidkina
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D013437:Carbon-Sulfur Lyases / Q000737:chemistry* / Q000378:metabolism; D016971:Citrobacter freundii / Q000201:enzymology*; D018360:Crystallography, X-Ray / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase