Phytoglycoprotein inhibits interleukin-1beta and interleukin-6 via p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated RAW 264.7 cells.
This study was carried out to investigate the anti-inflammatory effects of 30-kDa glycoprotein isolated from Dioscorea batatas Decne (DBD glycoprotein), which consists of carbohydrate content (61%) and protein content (39%) on lipopolysaccharide (LPS, 2 microg/ml)-stimulated RAW 264.7 cells. We found that DBD glycoprotein (200 microg/ml) has an inhibitory effect on the production of intracellular hydrogen peroxide (H(2)O(2)), on the phosphorylation of p38 mitogen-activated protein (MAP) kinase, on the DNA binding activity of activator protein-1 (AP-1), and on c-Jun and c-Fos protein expression, respectively. In addition, DBD glycoprotein treatment markedly suppressed the interleukin (IL)-1beta, IL-6, and inducible nitric oxide synthase (iNOS) expression and the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. Interestingly, IL-1beta, IL-6, and iNOS expression was significantly attenuated by treatment with protein kinase C (PKC) inhibitor (staurosporine) as well as p38 MAP kinase inhibitor (SKF86002) in LPS-stimulated RAW 264.7 cells. On the basis of these results, we assume that DBD glycoprotein has anti-inflammatory potential, which can modulate proinflammatory signal transduction in LPS-stimulated RAW 264.7 cells.
Lee
Sei-Jung
SJ
Molecular Biochemistry Laboratory, Biotechnology Research Institute and Center for the Control of Animal Hazards Using Biotechnology (BK 21), Chonnam National University, Kwang-ju, 300, Yongbong-Dong 500-757, South Korea.
Lim
Kye-Taek
KT
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D027742:Dioscorea / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*; D000093242:Interleukin-6 Inhibitors
D006023:Glycoproteins; D006861:Hydrogen Peroxide; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D048051:p38 Mitogen-Activated Protein Kinases; D000093242:Interleukin-6 Inhibitors