title: Dual restriction enzyme digest of cationic-gold-coated DNA scaffolds.
abstract: DNA strands coated with AuNPs were cleaved by restriction enzymes while in solution or on a surface. Enzymatic activity was verified by gel electrophoresis prior to surface analysis. Cleavage results suggest that enzymes can recognize the AuNP-coated strands while on the surfaces, though specificity in digestion has not yet been verified. Development allows for advances in site specific localization of components using biological media.
authors.affiliation: Department of Chemistry , Purdue University, West Lafayette, IN 47907, USA.
authors.forename: John W J
authors.initials: JW
authors.lastname: Slavin
authors.forename: Albena
authors.initials: A
authors.lastname: Ivanisevic
mesh_terms: D002412:Cations; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D002412:Cations; D020099:Coated Materials, Biocompatible; D006046:Gold; D004247:DNA; D004262:DNA Restriction Enzymes