title: Labeling of pancreatic islets with iron oxide nanoparticles for in vivo detection with magnetic resonance.
abstract: In vitro labeling of pancreatic islets with iron nanoparticles enables their direct posttransplant visualization by magnetic resonance; however, there is still a discrepancy in the fate of iron nanoparticles. This study was performed to detail the labeling process, consequently to improve the labeling efficacy and to confirm safety for islet cells. The islets were visible on T2*-weighted magnetic resonance images as hypointense spots immediately after 1-hr cultivation. Although at this time already the sufficient superparamagnetic effect was achieved, most of the particles were deposed in islet macrophages and only later were they found in endosomes of endocrine islet cells. The iron content depended on length of culture period. The labeled islets showed an intact ultrastructure, responded normally to glucose stimulation in vitro, and were able to treat experimental diabetes. For purpose of subsequent magnetic resonance imaging, a 24-hr culture with ferucarbotran leads to sufficient labeling with no apparent adverse effect on beta cell morphology or function.
authors.affiliation: Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Berkova
authors.forename: Daniel
authors.initials: D
authors.lastname: Jirak
authors.forename: Klara
authors.initials: K
authors.lastname: Zacharovova
authors.forename: Jan
authors.initials: J
authors.lastname: Kriz
authors.forename: Alena
authors.initials: A
authors.lastname: Lodererova
authors.forename: Peter
authors.initials: P
authors.lastname: Girman
authors.forename: Tomas
authors.initials: T
authors.lastname: Koblas
authors.forename: Eva
authors.initials: E
authors.lastname: Dovolilova
authors.forename: Marie
authors.initials: M
authors.lastname: Vancova
authors.forename: Milan
authors.initials: M
authors.lastname: Hajek
authors.forename: Frantisek
authors.initials: F
authors.lastname: Saudek
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005290:Ferric Compounds; D050417:Insulin-Secreting Cells / Q000473:pathology; D007515:Islets of Langerhans / Q000473:pathology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D008264:Macrophages / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods; D053768:Metal Nanoparticles; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide