title: ATRA-inhibited proliferation in glioma cells is associated with subcellular redistribution of beta-catenin via up-regulation of Axin.
abstract: Retinoic acid (RA) is a major chemopreventive agent which exerts strong anti-tumor activity partly by trans-repressing the Wnt/beta-catenin signaling pathway in some tumor cell lines. However, the definite mechanism of RA trans-repression of the Wnt/beta-catenin signaling pathway has not been elucidated clearly. In this work, we found that all-trans retinoic acid (ATRA) significantly inhibited proliferation of glioma cells, accompanied by up-regulation of expression of Axin and altered subcellular distribution of beta-catenin. Transfecting C6 cells with rAxin further confirmed that increased expression of Axin is obligate for inhibition of proliferation and the increase of the cytoplasmic beta-catenin. Our results suggested that Axin might play an important role in RA-mediated anti-proliferative effects of glioma cell lines.
authors.affiliation: Department of Pathology, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province 710032, PR China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Daqing
authors.initials: D
authors.lastname: Zhao
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.forename: Liying
authors.initials: L
authors.lastname: Zhang
authors.forename: Fanfan
authors.initials: F
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D060466:Axin Protein; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D021381:Protein Transport; D051381:Rats; D012097:Repressor Proteins / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D015854:Up-Regulation; D051176:beta Catenin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D060466:Axin Protein; D012097:Repressor Proteins; D051176:beta Catenin; D014212:Tretinoin