title: Screening enantioselective epoxide hydrolase activities from marine microorganisms: detection of activities in Erythrobacter spp.
abstract: To develop an enantioselective epoxide hydrolase (EHase) from marine microorganisms, marine samples were collected from a variety of marine environments. Strains isolated by the capability of living on styrene oxide (SO) were screened for retaining enantioselective EHase activities toward SO by combining spectrophotometric, GC, and HPLC analysis. Consequently, one strain, JCS358, was selected, and the sequence analysis of 16S rRNA gene showed that the strain belonged to Erythrobacter cluster. Twelve additional Erythrobacter strains from this study or acquired from culture collections were thereby tested for displaying EHase activities, and most of tested strains showed enantioselective hydrolysis toward SO and glycidyl phenyl ether. Kinetic resolution of racemic SO using whole cell of Erythrobacter sp. JCS358 was performed. Enantiopure (S)-SO could be obtained with an enantiomeric excess (ee) higher than 99% after 15 h incubation. The determination of 1-phenyl-1,2-ethanediol configuration derived from racemic SO confirmed the enantioselective hydrolyzing activity of Erythrobacter sp. JCS358.
authors.affiliation: Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan, South Korea.
authors.forename: Young-Ok
authors.initials: YO
authors.lastname: Hwang
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Woo
authors.forename: Kye Kyung
authors.initials: KK
authors.lastname: Kwon
authors.forename: Takako
authors.initials: T
authors.lastname: Sato
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
authors.forename: Myong Soo
authors.initials: MS
authors.lastname: Han
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D004777:Environment; D004851:Epoxide Hydrolases / Q000378:metabolism*; D004852:Epoxy Compounds / Q000378:metabolism*; D005820:Genetic Testing; D007700:Kinetics; D008386:Marine Biology; D008969:Molecular Sequence Data; D010802:Phylogeny; D042301:Sphingomonadaceae / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification
chemical_list: D004852:Epoxy Compounds; D004851:Epoxide Hydrolases