title: Comparative proteomics of human monkeypox and vaccinia intracellular mature and extracellular enveloped virions.
abstract: Orthopoxviruses are among the largest and most complex of the animal viruses. In response to the recent emergence of monkeypox in Africa and the threat of smallpox bioterrorism, two orthopoxviruses with different pathogenic potentials, human monkeypox virus and vaccinia virus, were proteomically compared with the goal of identifying proteins required for pathogenesis. Orthopoxviruses were grown in HeLa cells to two different viral forms (intracellular mature virus and extracellular enveloped virus), purified by sucrose gradient ultracentrifugation, denatured using RapiGest surfactant, and digested with trypsin. Unfractionated samples and strong cation exchange HPLC fractions were analyzed by high-resolution reversed-phase nano-LC-MS/MS, and analyses of the MS/MS spectra using SEQUEST and X! Tandem resulted in the confident identification of hundreds of monkeypox, vaccinia, and copurified host-cell proteins. The unfractionated samples were additionally analyzed by LC-MS using an LTQ-Orbitrap, and the accurate mass and elution time tag approach was used to perform quantitative comparisons. Possible pathophysiological roles of differentially abundant Orthopoxvirus proteins are discussed. Data, processed results, and protocols are available at http://www.proteomicsresource.org/.
authors.affiliation: Fundamental Science Division and Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Manes
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Estep
authors.forename: Heather M
authors.initials: HM
authors.lastname: Mottaz
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.forename: Therese R W
authors.initials: TR
authors.lastname: Clauss
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Monroe
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Du
authors.forename: Joshua N
authors.initials: JN
authors.lastname: Adkins
authors.forename: Scott W
authors.initials: SW
authors.lastname: Wong
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008993:Monkeypox virus / Q000737:chemistry*; D040901:Proteomics; D053719:Tandem Mass Spectrometry / Q000379:methods; D014616:Vaccinia virus / Q000737:chemistry*; D014771:Virion / Q000737:chemistry*