title: Retrograde signaling onto Ret during motor nerve terminal maturation.
abstract: Establishment of the neuromuscular synapse requires bidirectional signaling between the nerve and muscle. Although much is known on nerve-released signals onto the muscle, less is known of signals important for presynaptic maturation of the nerve terminal. Our results suggest that the Ret tyrosine kinase receptor transmits a signal in motor neuron synapses that contribute to motor neuron survival and synapse maturation at postnatal stages. Ret is localized specifically to the presynaptic membrane with its ligands, GDNF (glial cell line-derived neurotrophic factor)/NTN (neurturin), expressed in skeletal muscle tissue. Lack of Ret conditionally in cranial motor neurons results in a developmental deficit of maturation and specialization of presynaptic neuromuscular terminals. Regeneration of Ret-deficient adult hypoglossal motor neurons is unperturbed, but despite contact with the unaffected postsynaptic specializations, presynaptic axon terminal maturation is severely compromised in the absence of Ret signaling. Thus, Ret transmits a signal in motor nerve terminals that participate in the organization and maturation of presynaptic specializations during development and during regeneration in the adult.
authors.affiliation: Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, 171 77 Stockholm, Sweden.
authors.forename: Christel
authors.initials: C
authors.lastname: Baudet
authors.forename: Ester
authors.initials: E
authors.lastname: Pozas
authors.forename: Igor
authors.initials: I
authors.lastname: Adameyko
authors.forename: Elisabet
authors.initials: E
authors.lastname: Andersson
authors.forename: Johan
authors.initials: J
authors.lastname: Ericson
authors.forename: Patrik
authors.initials: P
authors.lastname: Ernfors
mesh_terms: D000818:Animals; D049108:Cell Enlargement; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology*; D017729:Presynaptic Terminals / Q000502:physiology*; D051096:Proto-Oncogene Proteins c-ret / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013569:Synapses / Q000502:physiology
chemical_list: D051096:Proto-Oncogene Proteins c-ret; C494999:Ret protein, mouse