title: Muscle study in experimental scoliosis in rabbits with costotransversectomy: evidence of ischemic process.
abstract: Scoliosis involves the central nervous system diseases, ligaments, articulations and skeletal muscles, but there is no consensus on its pathogeny and progression of muscle abnormalities. In this study, we investigate the morphologic changes in the muscle of rabbit submitted to experimental scoliosis, with special emphasis on abnormalities related to blood supply. We studied 26 rabbits subjected to costotransversectomy by pulling out six transverse processes at thoracic level and six rabbits were used as controls. All the animals operated upon developed scoliosis showing an average angle of 29.1 degrees on the 60th day, with its apices located at T4 and T12 when they were subjected to paraspinal muscle biopsy on both sides. The muscle biopsies were subjected to histological stains and histochemical reactions, as well as to a morphometric study. On the concave side, the changes were not statistically significant regarding the control group. On the convex side conjunctive tissue proliferation, infiltration by adipose tissue, central nucleus excess, inflammatory reaction, segmental fibrosis, type 1 fiber hypertrophy, type 2 fiber hypertrophy and atrophic angular dark fibers in the unspecific esterase were statistically significant. The segmental fibrosis reached a circumscribed muscle segment, compatible with an ischemic phenomenon. The histological diagnoses on the concave side of the animals had unspecific alterations (atrophy and hypertrophy) in 13, myopathy in 3, denervation in 3 and normal in 7. The convex side diagnoses were myopathy in 14, denervation in 8, mixed in 3 and normal in 1. The procedure determined morphologic changes on the convex side indicating possible denervation or myopathy of ischemic origin.
authors.affiliation: Department of Internal Medicine/Neurology, Universidade Federal do Paraná, Rua Gal. Carneiro 181-3 Andar, Curitiba, PR 80060-900, Brazil. werneck@ufpr.br
authors.forename: Lineu C
authors.initials: LC
authors.lastname: Werneck
authors.forename: Vlademir A
authors.initials: VA
authors.lastname: Cousseau
authors.forename: Xavier S
authors.initials: XS
authors.lastname: Graells
authors.forename: Mauricio C
authors.initials: MC
authors.lastname: Werneck
authors.forename: Rosana H
authors.initials: RH
authors.lastname: Scola
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007511:Ischemia / Q000209:etiology / Q000473:pathology*; D018485:Muscle Fibers, Skeletal / Q000473:pathology*; D018482:Muscle, Skeletal / Q000473:pathology*; D011817:Rabbits; D012600:Scoliosis / Q000150:complications / Q000473:pathology*