title: Distribution and activity of a Dippu DH31-like peptide in the large milkweed bug Oncopeltus fasciatus.
abstract: The milkweed bug, Oncopeltus fasciatus, is a plant feeding hemipteran. While there has been much research done on the neurohormonal control of the post-feeding diuresis in the blood-feeding hemipteran, Rhodnius prolixus, little is known about the control of the post-feeding diuresis in O. fasciatus. One of the neurohormones that may play a role in this rapid diuresis belongs to the calcitonin-like diuretic hormone (DH31) family of insect peptides. In this study we demonstrate the presence of DH31-like immunoreactivity in the central nervous system (CNS) and gut of O. fasciatus 5th instars. As well, DH31-like material was quantified and partially purified from the CNS of 5th instar O. fasciatus using reversed-phase liquid chromatography (RPLC) and monitored with an enzyme-linked immunosorbent assay (ELISA). When tested on O. fasciatus 5th instar Malpighian tubules, DH31-like peptides significantly increased the rate of secretion over saline controls. The results suggest that there is a DH31-like peptide(s) present in the CNS of O. fasciatus and that this peptide may play a role in the control of Malpighian tubule secretion.
authors.affiliation: Department of Biology, University of Toronto at Mississauga, Ontario, Canada L5L-1C6. victoria.tebrugge@utoronto.ca
authors.forename: V A
authors.initials: VA
authors.lastname: Te Brugge
authors.forename: I
authors.initials: I
authors.lastname: Orchard
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002490:Central Nervous System / Q000737:chemistry / Q000187:drug effects; D005724:Ganglia / Q000737:chemistry / Q000187:drug effects; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000187:drug effects; D020062:Heteroptera / Q000737:chemistry*; D008317:Malpighian Tubules / Q000187:drug effects* / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D018377:Neurotransmitter Agents; D015124:8-Bromo Cyclic Adenosine Monophosphate