Protective effects of resveratrol on UVB-irradiated HaCaT cells through attenuation of the caspase pathway.
Resveratrol, a polyphenol present in grapes and red wine, exerts wide biological properties including antioxidant, chemoprevention and cardioprotective effects. It was recently reported that resveratrol attenuates the oxidative DNA damage in neuronal cells. However, whether resveratrol attenuates the UVB-induced cell death in skin still remains largely unknown. In this study, to confirm the photoprotection properties of resveratrol, we investigated the effect of the pretreatment of resveratrol on UVB-treated HaCaT cells and molecular mechanisms concerning apoptotic pathways. Our data showed that HaCaT cells markedly decreased cell proliferation rates after UVB irradiation. Notably, the pretreatment of resveratrol on UVB irradiation resulted in an increase in cell survival which concomitantly reduced the reactive oxygen species production. Furthermore, the activation of caspase-3 and -8 was partially decreased in resveratrol-pretreated HaCaT cells, implying that the attenuation of caspase-3 and -8 activation is involved in cell survival after UVB irradiation. Thus, our data indicate that resveratrol may be a promising photoprotection agent, used in sunscreen products, to reduce cell death in UV-damaged skin.
Park
Kun
K
Department of Dermatology, College of Medicine, Eulji Hospital, Seoul, Korea. derma@chol.com
Lee
Ji-Hye
JH
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D061945:Caspase Inhibitors; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D019610:Cytoprotection; D006801:Humans; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
D000975:Antioxidants; D061945:Caspase Inhibitors; D017382:Reactive Oxygen Species; D013267:Stilbenes; D013473:Sunscreening Agents; D000077185:Resveratrol