Static magnetic field selects undifferentiated myelomonocytes from low-glutamine concentration stimulated U937 cells.
Reduced glutamine (GLN) concentration in the culture medium of a U937 cell line caused them to be differentiated along the monocytic pathway; cells attached to the matrix and to each other by extending pseudopodia and acquired specific functional characteristics, such as the expression of alpha-naphthyl-acetate esterase and the capacity to reduce nitroblue tetrazolium, as well as becoming active phagocytes. When U937 cells were differentiated under continuous exposure to a 6mT static magnetic field (MF) the overall differentiation process was perturbed. Surprisingly, after 5 days' exposure to the static MF, higher cell viability and differentiation were observed in cells cultured in a GLN-deprived medium than in cells grown in the same medium but in the absence of a static MF. The latter cells, particularly those that were still floating in the medium, were stimulated with TPA for a further 3 days. These cells differentiated and attached to the substrate. Conversely, the same treatment applied to cells cultured in GLN-deprived medium in the presence of the static MF resulted in resistance to TPA-induced differentiation. Indeed, these cells exhibited a round shape and in-suspension growth.
Tenuzzo
B
B
Department of Biological and Environmental Science and Technology, University of the Salento, Via per Monteroni, 73100 Lecce, Italy.
Dwikat
M
M
Dini
L
L
D001391:Azo Compounds; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005973:Glutamine / Q000494:pharmacology*; D006801:Humans; D008280:Magnetics; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D020298:U937 Cells
D001391:Azo Compounds; D005973:Glutamine; C033302:diformazan dye; D013755:Tetradecanoylphorbol Acetate