Lobeline effects on tonic and methamphetamine-induced dopamine release.
The mechanisms of interaction between lobeline and the dopamine transporter (DAT) or the vesicular monoamine transporter (VMAT-2) are not clear. The goal of this study was to elucidate the effects of lobeline on these transporters in a cell system co-expressing the DAT and VMAT-2. Lobeline caused release of [(3)H]dopamine to a similar extent as reserpine (VMAT-2 inhibitor), but was less efficacious than methamphetamine or dopamine. Additionally, lobeline decreased the [(3)H]dopamine-releasing effects of methamphetamine, unlike reserpine which increased release by methamphetamine. These results suggest that lobeline has unique properties at the DAT and VMAT-2 which may make it useful as a pharmacotherapeutic to treat methamphetamine abuse.
Wilhelm
Clare J
CJ
Research Service, Veterans Affairs Medical Center, Portland, OR 97239, USA.
Johnson
Robert A
RA
Eshleman
Amy J
AJ
Janowsky
Aaron
A
D019969:Amphetamine-Related Disorders / Q000188:drug therapy; D002460:Cell Line; D003042:Cocaine / Q000031:analogs & derivatives / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D015259:Dopamine Agents / Q000494:pharmacology*; D050483:Dopamine Plasma Membrane Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D006801:Humans; D008120:Lobeline / Q000494:pharmacology*; D008694:Methamphetamine / Q000494:pharmacology*; D009627:Nomifensine / Q000494:pharmacology; D012110:Reserpine / Q000494:pharmacology; D050493:Vesicular Monoamine Transport Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*
D015259:Dopamine Agents; D050483:Dopamine Plasma Membrane Transport Proteins; D018765:Dopamine Uptake Inhibitors; C493452:SLC18A2 protein, human; D050493:Vesicular Monoamine Transport Proteins; D009627:Nomifensine; D008694:Methamphetamine; C069723:2beta-carbomethoxy-3beta-(4-iodophenyl)tropane; D012110:Reserpine; D008120:Lobeline; D003042:Cocaine; D004298:Dopamine