title: Monocyte chemotactic protein-1 in the migration of differentiated leukaemic cells toward alveolar epithelial cells.
abstract: All-trans retinoic acid (ATRA) can induce acute respiratory distress syndrome in patients with acute promyelocytic leukaemia (APL). The current study investigated the role of monocyte chemotactic protein (MCP)-1 in the chemotactic transmigration of ATRA-treated NB4 (ATRA-NB4) APL cells toward A549 alveolar epithelial cells. NB4 and A549 cells were separately cultured with ATRA and/or dexamethasone (DEX). ATRA-NB4 cells were then placed in an upper insert and co-incubated with A549 cells or their conditioned medium (CM) located in a lower plate to test their transmigration activity. ATRA stimulated NB4 cells to transmigrate toward the A549 cells. The secretion of MCP-1 was enhanced by ATRA treatment in both A549 and NB4 cells. The binding assay demonstrated that ATRA-NB4 cells bound MCP-1. Pre-treatment of both CM-A549 cells with antibodies against MCP-1 and of ATRA-NB4 cells with antibodies against MCP-1 receptors reduced ATRA-NB4 cell transmigration. DEX did not suppress MCP-1 secretion and transmigration in ATRA-NB4 cells, although when applied to A549 cells, MCP-1 secretion was suppressed and ATRA-NB4 cell transmigration was attenuated. Monocyte chemotactic protein-1 secreted from alveolar epithelial cells plays an important role in the cell-cell interaction involved in the chemotactic transmigration of all-trans retinoic acid-treated acute promyelocytic leukaemia cells toward alveolar epithelial cells.
authors.affiliation: Dept of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China.
authors.forename: W-H
authors.initials: WH
authors.lastname: Tsai
authors.forename: C-H
authors.initials: CH
authors.lastname: Shih
authors.forename: C-C
authors.initials: CC
authors.lastname: Lin
authors.forename: C-K
authors.initials: CK
authors.lastname: Ho
authors.forename: F-C
authors.initials: FC
authors.lastname: Hsu
authors.forename: H-C
authors.initials: HC
authors.lastname: Hsu
mesh_terms: D000970:Antineoplastic Agents / Q000009:adverse effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects*; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000187:drug effects*; D042381:Granulocyte Precursor Cells / Q000187:drug effects*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects; D054390:Receptors, CCR2; D014212:Tretinoin / Q000009:adverse effects*
chemical_list: D000970:Antineoplastic Agents; C515334:CCR2 protein, human; D018932:Chemokine CCL2; D054390:Receptors, CCR2; D014212:Tretinoin