title: Effect of age on bone mineral density and micro architecture in the radius and tibia of horses: an Xtreme computed tomographic study.
abstract: BACKGROUND
  The effect of age on the bone mineral density and microarchitecture of the equine radius and tibia was investigated. Fifty-six bones from 15 horses aged four to 21 years were used. There were nine geldings and six mares, and none of the horses had any disease influencing bone properties. Xtreme computed tomography was used to evaluate a 9-mm segment of the diaphysis and metaphysis of each bone. The following variables were determined: length of the bone, circumference and diameter in the frontal and sagittal planes in the middle of the bone.Diaphysis: total volume, bone volume, bone volume ratio, slice area, bone area, marrow area, cortical and marrow thickness, bone mineral density, polar moment of inertia of the cortex.Metaphysis: total area, bone area, cortical bone area, cortical thickness, bone mineral density, bone mineral density in the cortex, bone mineral density in the trabecular region, trabecular number, trabecular thickness, trabecular separation, polar moment of inertia of the metaphysis, polar moment of inertia of the cortex of the metaphysis.
  
  
  RESULTS
  Bone density and microarchitecture were not affected by breed or gender. However, the microarchitecture varied with the age of the horse; the number of trabeculae decreased significantly and the distance between trabeculae increased significantly with increasing age. There were no significant differences between bones of the left and right limbs or between the radius and tibia.
  
  
  CONCLUSIONS
  The variables investigated did not differ between geldings and mares. However, there were age-related changes in the microstructure of the bones. Further experimental studies are necessary to determine whether these changes reduce bone strength. Age-related changes in the bones were seen and may explain the higher incidence of fractures and fissures in older horses.
authors.affiliation: Equine Hospital, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland. Afuerst@vetclinics.uzh.ch
authors.forename: A
authors.initials: A
authors.lastname: Fürst
authors.forename: D
authors.initials: D
authors.lastname: Meier
authors.forename: S
authors.initials: S
authors.lastname: Michel
authors.forename: A
authors.initials: A
authors.lastname: Schmidlin
authors.forename: L
authors.initials: L
authors.lastname: Held
authors.forename: A
authors.initials: A
authors.lastname: Laib
mesh_terms: D000367:Age Factors; D000818:Animals; D015519:Bone Density / Q000502:physiology*; D005260:Female; D006736:Horses / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D011884:Radius / Q000000981:diagnostic imaging / Q000502:physiology*; D012044:Regression Analysis; D013977:Tibia / Q000000981:diagnostic imaging / Q000502:physiology*; D014057:Tomography, X-Ray Computed / Q000379:methods / Q000662:veterinary*