title: Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.
abstract: The cell-based assay using yeast deletion mutants has been recognized as an efficient analysis to discover therapeutic compounds and reveal their mode of action. In this study, S. pombe deletion mutants-based HTS screening was carried out to identify potential anti-cancer agents. The NCI chemical library of 5700 compounds was screened using kit strains, which consisted of S. pombe mutants harboring deletions in genes involved in DNA repair and mitotic control. During the screening, we identified 40 compounds conferring growth inhibition of S. pombe. Their anti-tumorigenic properties were examined by phenotypic effect on S. pombe, flow cytometry and apoptosis analysis of human cancer. Here, we report hit compounds inducing apoptosis for development of anti-cancer agents suggesting that S. pombe deletion mutants are useful in identifying potential anti-cancer agents in human cancer therapeutics.
authors.affiliation: Functional Genomics, KRIBB, Yusong, Daejeon 305-806, South Korea.
authors.forename: Kyung-Sook
authors.initials: KS
authors.lastname: Chung
authors.forename: Nam-Hui
authors.initials: NH
authors.lastname: Yim
authors.forename: Seung-Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Shin-Jung
authors.initials: SJ
authors.lastname: Choi
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Hur
authors.forename: Kwang-Lae
authors.initials: KL
authors.lastname: Hoe
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Kim
authors.forename: Sondra
authors.initials: S
authors.lastname: Goehle
authors.forename: Hyung-Bae
authors.initials: HB
authors.lastname: Kim
authors.forename: Kyung-Bin
authors.initials: KB
authors.lastname: Song
authors.forename: Hyang-Sook
authors.initials: HS
authors.lastname: Yoo
authors.forename: Ki-Hwan
authors.initials: KH
authors.lastname: Bae
authors.forename: Julian
authors.initials: J
authors.lastname: Simon
authors.forename: Misun
authors.initials: M
authors.lastname: Won
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D009154:Mutation; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents