Synthesis of oligonucleotide derivatives using ChemMatrix supports.
The synthesis of oligonucleotides on poly(ethylene glycol)-based (ChemMatrix) supports was studied. Results show that oligonucleotides can be indeed prepared in good yields using slightly modified synthesis cycles and automated DNA synthesizers. The use of these supports for the synthesis of oligonucleotide-peptide conjugates and for the ligation of oligonucleotides using Cu(+)-catalyzed cycloadition reactions is reported. Moreover, these supports can be used for the preparation of oligonucleotides in anhydrous solvents, followed by hybridization of the complementary sequences in aqueous buffers.
Mazzini
Stefania
S
Institute for Research in Biomedicine, Barcelona Science Park, University of Barcelona, Spain.
García-Martin
Fayna
F
Alvira
Margarita
M
Aviñó
Anna
A
Manning
Brendan
B
Albericio
Fernando
F
Eritja
Ramon
R
D002384:Catalysis; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D004247:DNA / Q000737:chemistry; D004264:DNA Topoisomerases, Type I / Q000737:chemistry; D008024:Ligands; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D014867:Water / Q000737:chemistry
D008024:Ligands; D009841:Oligonucleotides; D010455:Peptides; D012996:Solutions; D014867:Water; D011092:Polyethylene Glycols; D003300:Copper; D004247:DNA; D004264:DNA Topoisomerases, Type I