title: Drosophila peroxiredoxin 5 is the second gene in a dicistronic operon.
abstract: We identified a Drosophila homologue that belongs to a subfamily of the mammalian peroxiredoxin 5 genes. The Drosophila peroxiredoxin 5 (dPrx 5) gene corresponds to CG7217 (FlyBase nomenclature), and exhibits identical polarity to that of the nearby upstream CG7215 gene, for which the deduced amino acid sequence reveals an ubiquitin-like domain at the N-terminus. The tandem pattern of the arrangement of these two genes is well-conserved among the Drosophilids. In addition to CG7215 and dPrx5 gene-specific transcripts that could be transcribed independently from two distinct promoters, transcripts spanning both coding regions have been identified, leading to the characterization of this gene cluster as a dicistronic operon. The different transcripts exhibit stage-specific accumulation patterns. While the upstream CG7215 gene can be expressed from both mono- and dicistronic mRNAs, the downstream dPrx5 gene is likely to be expressed predominantly from its monocistronic transcripts.
authors.affiliation: Department of Biological Sciences, Southern Methodist University, 6501 Airline, Dedman Life Sciences Building, Room 113, Dallas, TX 75275, USA.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Michalak
authors.forename: William C
authors.initials: WC
authors.lastname: Orr
authors.forename: Svetlana N
authors.initials: SN
authors.lastname: Radyuk
mesh_terms: D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D009928:Organ Specificity; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D054464:Peroxiredoxins