Soluble CD40 and its ligand CD154 in patients with Graves' ophthalmopathy during combined therapy with corticosteroids and teleradiotherapy.
OBJECTIVE
To assess the role of CD40/CD154 interaction in GO pathogenesis and to estimate usefulness of soluble CD40 (sCD40) and CD154 (sCDI54) measurements as markers of GO activity.


METHODS
61 individuals in 4 groups: (1) 15 euthyroid patients with clinical symptoms of ophthalmopathy (GO) who underwent corticosteroid therapy consisting of intravenous infusions of methylprednisolone (MP) and subsequent treatment with oral prednisone (P) and teleradiotherapy (TR); (2) 14 patients with hyperthyroid GD (GDtox); (3) 22 patients with GD in euthyreosis treated with methimazol (GDeu); (4) 10 healthy volunteers age and sex-matched to group 1-3. The serum samples were collected 24 hours before MP, 24 hours after MP, after TR and at the end of therapy. Serum CD40, CD154 and TPOab were determined by ELISA and TSHRab by RIA.


RESULTS
Serum concentrations of CD40 (in pg/ml) and CD154 (in ng/ml) were increased in GO patients: 84.9 (74.7-93.9) and 4.0 (2.5-7.3) respectively in comparison to controls (p < 0.001 and p < 0.05 respectively). Serum CD154 in GO group was elevated as compared to both hyperthyroid and euthyroid GD without clinical ophthalmopathy (p < 0.001 both). The sCD40/sCD154 quotient was significantly elevated during GO therapy with CS and TR in nonresponders after MP (p < 0.05) and at the end of the study (p < 0.01).


CONCLUSIONS
Our data suggest an important role of CD40/ CD154 interaction in the pathogenesis of autoimmune process leading to inflammatory infiltration in Graves' ophthalmopathy, however usefulness of sCD40 and sCD154 measurements in prediction of effects of GO treatment and its monitoring needs further investigations.
Myśliwiec
J
J
Department of Endocrinology, Diabetology and Internal Diseases, Medical University of Białystok, ul. M. Sklodowskiej-Curie 24 A, 15-276 Białystok, Poland. mysjan@poczta.onet.pl
Waligórski
D
D
Nikołajuk
A
A
Górska
M
M
D000305:Adrenal Cortex Hormones / Q000627:therapeutic use*; D000328:Adult; D000368:Aged; D000917:Antibody Formation; D015551:Autoimmunity; D015415:Biomarkers / Q000097:blood*; D019013:CD40 Antigens / Q000097:blood / Q000737:chemistry*; D023201:CD40 Ligand / Q000097:blood / Q000737:chemistry*; D005260:Female; D049970:Graves Ophthalmopathy / Q000097:blood / Q000276:immunology*; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D011878:Radiotherapy / Q000379:methods
D000305:Adrenal Cortex Hormones; D015415:Biomarkers; D019013:CD40 Antigens; D023201:CD40 Ligand