title: Role of jasmonic acid in alteration of ginsenoside heterogeneity in elicited cell cultures of Panax notoginseng.
abstract: The efficient manipulation of ginsenoside heterogeneity of Panax notoginseng cells using a recently synthesized elicitor, 2-hydroxyethyl jasmonate (HEJ, at 200 microM), has been reported. In this work, the activities of two enzymes related to ginsenoside heterogeneity (distribution), protopanaxdiol 6-hydroxylase (P6H) and UDPG-ginsenoside Rd glucosyltransferase (UGRdGT), were examined in cell cultures of P. notoginseng elicited by HEJ. P6H and UGRdGT activities were increased by HEJ with corresponding changes in Rb/Rg ratio and Rb1/Rd ratio. Endogenous jasmonic acid (JA) seemed to mediate the induction of UGRdGT activation, but was not involved in P6H activation. The results suggest that JA, as a signal transducer, may play an important role in the alteration of ginsenoside heterogeneity in elicited P. notoginseng cells.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Feng-Xian
authors.initials: FX
authors.lastname: Hu
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhong
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D036145:Ginsenosides / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D053723:Panax notoginseng / Q000187:drug effects / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D036145:Ginsenosides; D054883:Oxylipins; C011006:jasmonic acid; D006899:Mixed Function Oxygenases; D005964:Glucosyltransferases