title: TSOL18/HP6-Tsol, an immunogenic Taenia solium oncospheral adhesion protein and potential protective antigen.
abstract: In this study, we employed Taenia solium mRNA extracted from a tapeworm of Venezuelan origin to clone express and test the recombinant protein of the T. solium homologue of the 18-kDa oncospheral adhesion molecule of Taenia saginata (HP6-Tsag/TSA18). We first confirm the conserved nature of the sequence of the T. solium homologue (TSOL18/HP6-Tsol) and demonstrate that the recombinant protein, which, as with its T. saginata homologue, is characterised by a fibronectin type III homology region, functions as an adhesion molecule. This emphasises the possible importance of TSOL18/HP6-Tsol in tissue invasion, thus providing a rational explanation for its efficacy as a vaccine. As protection against Taenia spp., oncospheres is antibody mediated, logically, therefore, TSOL18/HP6-Tsol may also serve as a diagnostic antigen, as is indeed the case for recombinant HP6-Tsag/TSA18.
authors.affiliation: Instituto Gulbenkian de Ciencia, Rua Quinta Grande 6, Apartado 14, P-2780-156 Oeiras Codex, Portugal. parkhous@igc.gulbenkian.pt
authors.forename: R Michael E
authors.initials: RM
authors.lastname: Parkhouse
authors.forename: Pedro
authors.initials: P
authors.lastname: Bonay
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: González
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ferrer
authors.forename: Teresa
authors.initials: T
authors.lastname: Gárate
authors.forename: Cruz M
authors.initials: CM
authors.lastname: Aguilar
authors.forename: Milagros M
authors.initials: MM
authors.lastname: Cortez A
authors.forename: Leslie J S
authors.initials: LJ
authors.lastname: Harrison
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019556:COS Cells; D002448:Cell Adhesion; D002522:Chlorocebus aethiops; D006801:Humans; D007668:Kidney / Q000166:cytology; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D041201:Taenia solium / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; C464926:HP6 protein, Taenia saginata; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C113407:TSOL-18 antigen