title: Original normal-phase high-performance liquid chromatographic separation of monoacylglycerol classes from extra virgin olive oil triacylglycerols for their stereospecific analysis.
abstract: An innovative procedure to separate the 3 isomeric sn-monoacylglycerols (MAG) classes (sn-1-, sn-2-, sn-3-MAG) is described. MAGs, obtained by chemical deacylation of triacylglycerols (TAGs), have been derivatized with (S)-(+)-1-(1-naphtyl)ethyl-isocyanate, and the resulting urethane derivatives have been separated by normal-phase high-performance liquid chromatography. This procedure allows resolution as diasteroisomers of the 2 enantiomeric classes (sn-1-MAG and sn-3-MAG), without the need of a chiral column, and to separate also the isomeric sn-2-MAG class; moreover, by introducing a chromophoric moiety, this reagent makes possible the ultraviolet detection of the analyte molecules. This procedure has been used to obtain the stereospecific analysis of the TAG fraction of extra virgin olive oil samples. The use of a nondestructive detector permitted the collection of the individual urethane classes; the fatty acid composition of each was determined by high-resolution gas chromatography, obtaining directly from the data the fatty acid distribution within each sn- position of TAGs. To validate this new method, the results have been compared with those obtained by 2 other procedures for TAG stereospecific analysis, and the obtained results were satisfactory since the proposed method gave data very similar to the other procedures.
authors.affiliation: University of Perugia, Department of Economic Science and Food Sciences, Section of Food Chemistry and Analysis, Via San Costanzo, I-06126 Perugia, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Petrosino
authors.forename: Roberto
authors.initials: R
authors.lastname: Riccieri
authors.forename: Francesca
authors.initials: F
authors.lastname: Blasi
authors.forename: Monia
authors.initials: M
authors.lastname: Brutti
authors.forename: Gilda
authors.initials: G
authors.lastname: D'Arco
authors.forename: Ancilla
authors.initials: A
authors.lastname: Bosi
authors.forename: Silvia
authors.initials: S
authors.lastname: Maurelli
authors.forename: Lina
authors.initials: L
authors.lastname: Cossignani
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Simonetti
authors.forename: Pietro
authors.initials: P
authors.lastname: Damiani
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D050178:Monoglycerides / Q000032:analysis*; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis*; D012015:Reference Standards; D012997:Solvents; D013237:Stereoisomerism; D014520:Urethane / Q000032:analysis
chemical_list: D007202:Indicators and Reagents; D050178:Monoglycerides; D000069463:Olive Oil; D010938:Plant Oils; D012997:Solvents; D014520:Urethane