title: Primary neurons become less susceptible to coxsackievirus B5 following maturation: the correlation with the decreased level of CAR expression on cell surface.
abstract: Coxsackievirus B (CVB) is one of the major pathogens of aseptic meningitis and meningioencephalitis, particularly in newborn infants. To analyze the influence of neural maturation on susceptibility to CVB infection, we prepared immature and mature neurons from 16-day-old BALB/c embryonic cortex. In contrast to immature neurons, mature neurons were less susceptible to CVB5 infection, as indicated by the decrease of cytopathic features. In mature neurons, progeny virus production was significantly hindered, and virus capsid protein VP1 synthesis and virus genome amplification were concomitantly reduced. In addition, the expression of coxsackievirus and adenovirus receptor (CAR), the major receptor of CVB5, was down-regulated in mature neurons. The antibody treatment specific to CAR significantly attenuated CVB5 susceptibility of immature neurons. These findings demonstrate that mature neurons become less susceptible to CVB by the decrease of CAR level. Thus, the data strongly support the idea that the level of virus receptor in neurons is one of the crucial determinants in the age-dependency of CVB virulence in central nervous system.
authors.affiliation: Department of Microbiology, University of Ulsan College of Medicine, Seoul, Korea.
authors.forename: Jeonghyun
authors.initials: J
authors.lastname: Ahn
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jee
authors.forename: Ilseon
authors.initials: I
authors.lastname: Seo
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Yoon
authors.forename: DongHou
authors.initials: D
authors.lastname: Kim
authors.forename: Yoo Kyum
authors.initials: YK
authors.lastname: Kim
authors.forename: Heuiran
authors.initials: H
authors.lastname: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D003588:Cytopathogenic Effect, Viral; D004198:Disease Susceptibility; D015536:Down-Regulation; D029822:Enterovirus B, Human / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011991:Receptors, Virus / Q000276:immunology / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: C568771:CLMP protein, human; C568772:CLMP protein, mouse; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D011991:Receptors, Virus