title: Effect of plasmapheresis on the immune system in endotoxin-induced sepsis.
abstract: BACKGROUND
  It has been proposed that plasmapheresis is most effective when applied early in Gram-negative sepsis. We therefore studied the effect of early plasmapheresis on immunity in experimental Escherichia coli endotoxin-induced sepsis.
  
  
  METHODS
  20 pigs received 30 microg/kg of E. coli endotoxin. 40 min later, half of the pigs were treated with plasmapheresis which lasted 4 h. The adhesion molecules, the oxidative burst, the number of neutrophils in blood and lungs, and cytokines were measured.
  
  
  RESULTS
  Infusion of endotoxin was associated with activation of adhesion molecules increased oxidative burst, increased concentration of cytokine, and accumulation of granulocytes in lung tissue. Plasmapheresis reduced the oxidative burst, and there was a tendency towards a reduced accumulation of granulocytes in the lung.
  
  
  CONCLUSIONS
  Though plasmapheresis was initiated early after the endotoxin infusion, it only temporarily attenuated a part of the activated cell-mediated immunity.
authors.affiliation: Department of Anaesthesiology and Intensive Care, Odense University Hospital, Odense, Denmark. palle.toft@ouh.regionsyddanmark.dk
authors.forename: P
authors.initials: P
authors.lastname: Toft
authors.forename: R
authors.initials: R
authors.lastname: Schmidt
authors.forename: A C
authors.initials: AC
authors.lastname: Broechner
authors.forename: B U
authors.initials: BU
authors.lastname: Nielsen
authors.forename: P
authors.initials: P
authors.lastname: Bollen
authors.forename: K E
authors.initials: KE
authors.lastname: Olsen
mesh_terms: D000818:Animals; D001773:Blood Cells / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004731:Endotoxins / Q000008:administration & dosage / Q000009:adverse effects*; D006098:Granulocytes; D007107:Immune System / Q000276:immunology / Q000473:pathology; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology; D009504:Neutrophils / Q000473:pathology; D010956:Plasmapheresis; D016897:Respiratory Burst; D018805:Sepsis / Q000139:chemically induced / Q000628:therapy*; D013552:Swine
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D004731:Endotoxins; C044101:endotoxin, Escherichia coli