Current approaches for engineering proteins with diverse biological properties.
In the past two decades, protein engineering has advanced significantly with the emergence of new chemical and genetic approaches. Modification and recombination of existing proteins not only produced novel enzymes used commercially and in research laboratories, but furthermore, they revealed the mechanisms of protein function. In this chapter, we will describe the applications and significance of current protein engineering approaches.
Li
Isaac T S
IT
Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada.
Pham
Elizabeth
E
Truong
Kevin
K
D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001665:Binding Sites; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000737:chemistry; D005658:Fungi / Q000378:metabolism; D005821:Genetic Techniques; D006801:Humans; D008957:Models, Genetic; D016296:Mutagenesis; D009841:Oligonucleotides / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D011995:Recombination, Genetic
D009841:Oligonucleotides; D011506:Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA