Enhanced fluorescence detection of cis-combretastatins by post-column photolysis.
A method is described to enhance the sensitivity of fluorescence detection of cis-combretastatins using a short post-column photolysis coil with a mercury lamp, by inducing the rapid conversion to the trans isomer. Although all the compounds studied showed enhanced fluorescence after photolysis, there were large differences in the absolute level, with the inherent response of the catechol CA1 being much lower than the corresponding phenolic CA4. Brief exposure to the deuterium lamp in a photodiode array detector also resulted in significant enhancement.
Stratford
Michael R L
MR
University of Oxford, Gray Cancer Institute, PO Box 100, Mount Vernon Hospital, Northwood, Middlesex HA6 2JR, UK. stratford@gci.ac.uk
D001632:Bibenzyls / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010782:Photolysis; D013050:Spectrometry, Fluorescence; D013267:Stilbenes / Q000032:analysis*
D001632:Bibenzyls; D013267:Stilbenes; C040105:combretastatin