title: Peripheral glucose metabolism is altered by epileptic seizures.
abstract: The aim of the present study was to investigate the status of jejunal absorption and peripheral metabolism of glucose in Wistar Audiogenic Rats (WAR), a genetic model of epilepsy, after seizures induced by intensive sound exposure. The jejunal loop of rats was isolated and infused (0.5 mL min(-1)) with Tyrode solution containing twice the normal concentrations of glucose, sodium, and potassium. Samples were taken at 5 or 10-min intervals over a 40-min period. At the end of the experiment, samples of liver and gastrocnemius muscle were taken to measure the levels of glycogen, glucose-6-phosphate, fructose-6-phosphate and glucose transporter-4 (GLUT4). Hepatic glucose-6-phosphate increased in WAR submitted to audiogenic seizure (21.90 +/- 3.08) as compared to non-susceptible Wistar rats (8.12 +/- 0.87) and to WAR not submitted to audiogenic stimulation (5.17 +/- 0.97). In addition, an increase in hepatic fructose-6-phosphate, an intermediate metabolite of the glycolytic pathway, was observed in WAR submitted to audiogenic seizure (5.98 +/- 0.99) compared to non-susceptible Wistar rats (2.38 +/- 0.53). According to the present results, jejunal absorption of glucose was not changed by seizures. However, generalized tonic-clonic seizures produced by sound stimulation resulted in a decrease in muscle glycogen content. In addition, our results demonstrated that the concentration of GLUT4 in the gastrocnemius muscle of WAR was 1.6-fold higher than that observed in resistant rats and that the audiogenic stimulus led to decreased concentration of this receptor in the muscle of WAR animals.
authors.affiliation: Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fernanda K S
authors.initials: FK
authors.lastname: Pereira
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Neves
authors.forename: Mércia P
authors.initials: MP
authors.lastname: Lima
authors.forename: Almir A
authors.initials: AA
authors.lastname: Braga
authors.forename: Jorge Luiz
authors.initials: JL
authors.lastname: Pesquero
authors.forename: Maria Carolina
authors.initials: MC
authors.lastname: Doretto
authors.forename: Elizabeth L
authors.initials: EL
authors.lastname: Borges
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D015153:Blotting, Western; D020195:Epilepsy, Reflex / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000378:metabolism*
chemical_list: D005636:Fructosephosphates; D051275:Glucose Transporter Type 4; D008112:Liver Glycogen; D019344:Lactic Acid; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; D006003:Glycogen; D005947:Glucose