High-intensity electric pulses induce mitochondria-dependent apoptosis in ovarian cancer xenograft mice.
Human ovarian cancer models were established in nude mice by transplanting SKOV(3) cells, and then tumors were exposed to high-intensity electric pulses with a voltage 1000 V, frequency of 1000 Hz, and duration of 250 ns for 1 min. Mitochondria permeability transition pore (PTP) was inspected by cofocal microscope; cytochrome C (Cyt C) and apoptosis-induced factor (AIF) were determined by immunohistochemistry; and voltage-dependent anion channel (VDAC) was measured by immunofluorescence. High-intensity electric pulses exposure led to increases of PTP, Cyt C, and AIF and a decrease of VDAC. These findings revealed that high-intensity electric pulses activated mitochondria electroporation, apoptosis was realized via mitochondria pathway.
Li
C
C
Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
Hu
L-N
LN
Dong
X-J
XJ
Sun
C-X
CX
Mi
Y
Y
D000818:Animals; D017209:Apoptosis; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D018274:Electroporation; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D051336:Mitochondrial Membranes; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D010539:Permeability; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
D051033:Apoptosis Inducing Factor; D050994:Voltage-Dependent Anion Channels; D045304:Cytochromes c