title: Vitellogenesis in both sexes of gonochoristic mud shrimp, Upogebia major (Crustacea): analyses of vitellogenin gene expression and vitellogenin processing.
abstract: The mud shrimp, Upogebia major is a gonochoristic species with distinct sexual dimorphism; however, the male has the "ovarian part of testis" in the gonad and mature-looking eggs appear in a similar reproductive cycle to the female. Vitellogenesis of U. major was investigated focusing on the characterization of vitellogenin (Vg) gene expression and Vg processing. Vg cDNA cloned by PCR-based methods was 7,799 bp-long, encoding 2,568 amino acids in a single open reading frame. The deduced amino acid sequence shared common characteristics conserved in other shrimp Vgs. The Vg gene was expressed in the hepatopancreas of females and males, the ovary, and the ovarian part of testis. Vitellins (Vns) were detected in the gonads of both females and males as three prominent polypeptides with apparent molecular masses of 82 kDa, 100 kDa, and 115 kDa. N-terminal amino acid sequences determined for the three polypeptides were present in the deduced amino acid sequence, demonstrating that they derived from a single long Vg polypeptide. Immunoblot analysis using polyclonal antibodies raised against two Vns (82 kDa and 100 kDa) confirmed Vg processing in the hepatopancreas, in the hemolymph and possibly in the oocytes, similarly in both sexes.
authors.affiliation: Laboratory of Animal Behavior and Evolution, Graduate School of Natural Science and Technology, Okayama University, Tsushima 3-1-1, Okayama 700-8530, Japan.
authors.forename: B J
authors.initials: BJ
authors.lastname: Kang
authors.forename: T
authors.initials: T
authors.lastname: Nanri
authors.forename: J M
authors.initials: JM
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: C-H
authors.initials: CH
authors.lastname: Han
authors.forename: M
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: M
authors.initials: M
authors.lastname: Saigusa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D033364:Decapoda / Q000033:anatomy & histology / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000378:metabolism; D043143:Hepatopancreas / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012727:Sex Characteristics; D014818:Vitellogenesis / Q000235:genetics*; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D014819:Vitellogenins