A Wnt-FoxQ2-nodal pathway links primary and secondary axis specification in sea urchin embryos.
The primary (animal-vegetal) (AV) and secondary (oral-aboral) (OA) axes of sea urchin embryos are established by distinct regulatory pathways. However, because experimental perturbations of AV patterning also invariably disrupt OA patterning and radialize the embryo, these two axes must be mechanistically linked. Here we show that FoxQ2, which is progressively restricted to the animal plate during cleavage stages, provides this linkage. When AV patterning is prevented by blocking the nuclear function of beta-catenin, the animal plate where FoxQ2 is expressed expands throughout the future ectoderm, and expression of nodal, which initiates OA polarity, is blocked. Surprisingly, nodal transcription and OA differentiation are rescued simply by inhibiting FoxQ2 translation. Therefore, restriction of FoxQ2 to the animal plate is a crucial element of canonical Wnt signaling that coordinates patterning along the AV axis with the initiation of OA specification.
Yaguchi
Shunsuke
S
National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. yaguchis@mail.nih.gov
Yaguchi
Junko
J
Angerer
Robert C
RC
Angerer
Lynne M
LM
D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D004475:Ectoderm / Q000254:growth & development / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D012333:RNA, Messenger / Q000235:genetics; D012617:Sea Urchins / Q000196:embryology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D051153:Wnt Proteins / Q000235:genetics / Q000502:physiology*; D051176:beta Catenin / Q000502:physiology
D012333:RNA, Messenger; D014157:Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin