Alteration of N-glycans related to articular cartilage deterioration after anterior cruciate ligament transection in rabbits.
OBJECTIVE
Osteoarthritis (OA) is the most common of all joint diseases, but the molecular basis of its onset and progression is controversial. Several studies have shown that modifications of N-glycans contribute to pathogenesis. However, little attention has been paid to N-glycan modifications seen in articular cartilage. The goal of this study was to identify disease specific N-glycan expression profiles in degenerated cartilage in a rabbit OA model induced by anterior cruciate ligament transection (ACLT).


METHODS
Cartilage samples were harvested at 7, 10, 14, and 28 days after ACLT and assessed for cartilage degeneration and alteration in N-glycans. N-Glycans from cartilage were analyzed by high performance liquid chromatography and mass spectrometry.


RESULTS
Histological analysis showed that osteoarthritic changes in cartilage occurred 10 days after ACLT. Apparent alterations in the N-glycan peak pattern in cartilage samples were observed 7 days after ACLT, and overall N-glycan changes in OA reflected alterations in both sialylation and fucosylation. These changes apparently preceded histological changes in cartilage.


CONCLUSIONS
These results indicate that changes in the expression of N-glycans are correlated with OA in an animal model. Understanding mechanisms underlying changes in N-glycans seen in OA may be of therapeutic value in treating cartilage deterioration.
Matsuhashi
T
T
Department of Orthopaedic Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
Iwasaki
N
N
Nakagawa
H
H
Hato
M
M
Kurogochi
M
M
Majima
T
T
Minami
A
A
Nishimura
S-I
SI
D000818:Animals; D016118:Anterior Cruciate Ligament; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology; D002358:Cartilage, Articular / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005269:Femur / Q000473:pathology; D015394:Molecular Structure; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013977:Tibia / Q000473:pathology
D011134:Polysaccharides