title: Expression of highly sulfated keratan sulfate synthesized in human glioblastoma cells.
abstract: Keratan sulfate (KS) proteoglycan is expressed in the extracellular matrix or cell surface in numerous tissues, predominantly in those of the cornea, cartilage, and brain. However, its structure, function, and regulation remain poorly understood. Our investigation of KS expression in glioblastoma cell lines using Western-blot and flow cytometry with anti-KS antibody (5D4) revealed that LN229 glioblastoma cell highly expresses KS on a cell surface. Real-time PCR analysis showed that LN229 expresses a high level of keratan sulfate Gal-6-sulfotransferase. Results of this study also demonstrate that recombinant 5D4-reactive aggrecan is produced in LN229. Taken together, these results suggest that LN229 produces 5D4-reactive highly sulfated KS and is useful to investigate the KS structure and function in glioblastoma.
authors.affiliation: Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8568, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Hayatsu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogasawara
authors.forename: Mika Kato
authors.initials: MK
authors.lastname: Kaneko
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
mesh_terms: D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D007632:Keratan Sulfate / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism*
chemical_list: D011509:Proteoglycans; D013431:Sulfates; D007632:Keratan Sulfate