Pancreatic mass, cellularity, and alpha-amylase and trypsin activity in feedlot steers fed diets differing in crude protein concentration.
Twenty-four yearling beef steers (initial BW = 510 +/- 4.9 kg) predominantly of Angus breeding were used in a randomized complete block design to determine the effect of dietary CP concentration on pancreatic cellularity, mass, and alpha-amylase and trypsin activities. Treatment diets were formulated to contain 8.8, 11.0, 13.2, and 15.4% CP. Soybean meal and Top Soy (ruminal bypass soybean meal) were used as supplemental protein sources to ensure that MP intake was increased with increasing dietary CP concentrations. Steers were penned in groups of 4 (1 steer per treatment) and individually fed at 2.5x the NE(m) requirement by using Calan gates for 28 d before tissue collection. Four steers (1 pen) were slaughtered per week. Pancreases were weighed, subsampled, frozen in liquid N(2), and stored at -80 degrees C until analyses for DNA, RNA, and protein concentrations, and alpha-amylase and trypsin activities. Pancreatic weight (g and g/kg of BW) did not differ among treatment groups. Pancreatic DNA concentration (mg/g) decreased linearly (P = 0.06) with increasing CP concentration. Pancreatic protein (g/pancreas) increased linearly (P = 0.08) with increasing dietary CP concentration. Pancreatic alpha-amylase activity (U/g, U/mg of DNA, U/g of protein, U/pancreas, and U/kg of BW) increased linearly (P < or = 0.04) with increasing dietary CP concentration. Pancreatic trypsin activity (U/g, U/g of DNA, U/g of protein, U/pancreas, and U/kg of BW) increased linearly (P < or = 0.09) with increasing dietary CP concentration. Pancreatic alpha-amylase and trypsin activities (U/mg of RNA) responded quadratically (P < or = 0.09), with the greatest alpha-amylase activity observed in the 13.2% CP treatment. These data indicate that increasing dietary CP concentration decreases pancreatic cell numbers and also increases the concentration and content of pancreatic alpha-amylase and trypsin activities. Changes in cell number and size may be important factors regulating digestive enzyme production in the pancreas of cattle.
Swanson
K C
KC
Department of Animal and Poultry Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1. kswanson@uoguelph.ca
Kelly
N
N
Salim
H
H
Wang
Y J
YJ
Holligan
S
S
Fan
M Z
MZ
McBride
B W
BW
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004044:Dietary Proteins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D009751:Nutritional Requirements; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000033:anatomy & histology / Q000166:cytology* / Q000201:enzymology*; D012313:RNA / Q000378:metabolism; D011897:Random Allocation; D012417:Rumen / Q000378:metabolism; D013025:Glycine max; D014357:Trypsin / Q000378:metabolism*; D000516:alpha-Amylases / Q000378:metabolism*
D004044:Dietary Proteins; D012313:RNA; D004247:DNA; D000516:alpha-Amylases; D014357:Trypsin