title: Genome-wide, high-resolution DNA methylation profiling using bisulfite-mediated cytosine conversion.
abstract: Methylation of cytosines ((m)C) is essential for epigenetic gene regulation in plants and mammals. Aberrant (m)C patterns are associated with heritable developmental abnormalities in plants and with cancer in mammals. We have developed a genome-wide DNA methylation profiling technology employing a novel amplification step for DNA subjected to bisulfite-mediated cytosine conversion. The methylation patterns detected are not only consistent with previous results obtained with (m)C immunoprecipitation (mCIP) techniques, but also demonstrated improved resolution and sensitivity. The technology, named BiMP (for Bisulfite Methylation Profiling), is more cost-effective than mCIP and requires as little as 100 ng of Arabidopsis DNA.
authors.affiliation: Laboratory of Plant Genetics, Department of Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland. jon.reinders@bioveg.unige.ch
authors.forename: Jon
authors.initials: J
authors.lastname: Reinders
authors.forename: Céline
authors.initials: C
authors.lastname: Delucinge Vivier
authors.forename: Grégory
authors.initials: G
authors.lastname: Theiler
authors.forename: Didier
authors.initials: D
authors.lastname: Chollet
authors.forename: Patrick
authors.initials: P
authors.lastname: Descombes
authors.forename: Jerzy
authors.initials: J
authors.lastname: Paszkowski
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D003596:Cytosine / Q000737:chemistry*; D019175:DNA Methylation; D023281:Genomics / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic; D013447:Sulfites / Q000737:chemistry*
chemical_list: D013447:Sulfites; D003596:Cytosine; C042345:hydrogen sulfite