title: A reducing-end-acting chitinase from Vibrio proteolyticus belonging to glycoside hydrolase family 19.
abstract: A chitinase gene belonging to the glycoside hydrolase family 19 from Vibrio proteolyticus (chi19) was cloned. The recombinant enzyme (Chi19) showed weak activities against polymeric substrates and considerable activities against fully N-acetylated chitooligosaccharides, (GlcNAc)(n), whose degree of polymerization was greater than or equal to five. It hydrolyzed (GlcNAc)(n) at the second linkage position from the reducing ends of the chitooligosaccharides. The hydrolytic products of colloidal chitin were mainly (GlcNAc)(2) from the initial stage of the reaction. The hydrolytic pattern of reduced colloidal chitin clearly suggested that the enzyme hydrolyzed the polymeric substrate from the reducing end.
authors.affiliation: National Food Research Institute, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Honda
authors.forename: Hajime
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Motomitsu
authors.initials: M
authors.lastname: Kitaoka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001704:Biopolymers / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D004795:Enzyme Stability; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D013379:Substrate Specificity; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D001704:Biopolymers; D002686:Chitin; D006026:Glycoside Hydrolases; D002688:Chitinases