title: Separation and determination of enkephalin-related peptides by capillary electrophoresis with amperometric detection.
abstract: A simple, reliable, and reproducible method for separation and determination of five enkephalin-related peptides based on CE with amperometric detection (AD) is described in this paper. A potential of 1.0 V was applied to the carbon disk electrode, which was used as a working electrode in this system. At 15 kV of applied voltage, the five compounds were separated within 18 min in a 20 mmol/L phosphate buffer solution (PBS, pH 7.6) including 2.5% methanol v/v. LOD for five enkephalins were ranged from 6.31 to 54.3 nmol/L. The method was applied successfully to determine the five compounds added in the human cerebrospinal fluid (CSF) and the recoveries were in the range of 95.8-98.2%.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fujian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Sheyue
authors.initials: S
authors.lastname: Yao
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D002244:Carbon / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D004745:Enkephalins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004745:Enkephalins; D010455:Peptides; D002244:Carbon