Microarray technology for use in molecular epidemiology.
Microarrays are a powerful laboratory tool for the simultaneous assessment of the activity of thousands genes. Remarkable advances in biological sample collection, preparation and automation of hybridization have enabled the application of microarray technology to large, population-based studies. Now, microarrays have the potential to serve as screening tools for the detection of altered gene expression activity that might contribute to diseases in human populations. Reproducible and reliable microarray results depend on multiple factors. In this chapter, biological sample parameters are introduced that should be considered for any microarray experiment. Then, the microarray technology that we have successfully applied to limited biological sample from all our molecular epidemiology studies is detailed. This reproducible and reliable approach for using microarrays should be applicable to any biological questions asked.
Vernon
Suzanne D
SD
Division of Viral and Rickettsial Diseases, National Centers for Infectious Diseases, Center for Disease Control and Prevention, Atlanta, GA, USA.
Whistler
Toni
T
D020869:Gene Expression Profiling; D006801:Humans; D017720:Molecular Epidemiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000235:genetics*
D012313:RNA