Yeast and mammalian two-hybrid systems for studying protein-protein interactions.
An important step in the analysis of protein function is identification of the interaction partners of each protein. The two-hybrid system has been widely used to identify and explore protein-protein interactions. By using various two-hybrid systems, numerous protein interactions that regulate apoptosis signaling have been discovered that reveal unexpected functions of cancer-relevant proteins. Methods for performing two-hybrid experiments using either yeast or mammalian cells will be described in this chapter.
Matsuzawa
Shu-ichi
S
Burnham Institute for Medical Research, La Jolla, CA, USA.
Reed
John C
JC
D000818:Animals; D006801:Humans; D007763:Lac Operon; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D020798:Two-Hybrid System Techniques
D011506:Proteins