title: Interaction of syntenin-1 and the NG2 proteoglycan in migratory oligodendrocyte precursor cells.
abstract: Migration of oligodendrocyte precursors along axons is a necessary prerequisite for myelination, but little is known about underlying mechanisms. NG2 is a large membrane proteoglycan implicated in oligodendrocyte migration. Here we show that a PDZ domain protein termed syntenin-1 interacts with NG2 and that syntenin-1 is necessary for normal rates of migration. The association of syntenin-1 with NG2, identified in a yeast two-hybrid screen, was confirmed by colocalization of both proteins within processes of oligodendroglial precursor cells and by coimmunoprecipitation from cell extracts. Syntenin-1 also colocalizes with NG2 in "co-capping" assays, demonstrating a lateral association of both proteins in live oligodendrocytes. RNA interference-mediated down-regulation of syntenin-1 in glial cells results in a significant reduction of migration in vitro, as does the presence of polyclonal antibody against NG2. Thus syntenin plays a role in the migration of oligodendroglial precursors, and we suggest that NG2-syntenin-1 interactions contribute to this.
authors.affiliation: Molecular Cell Biology, Department of Biology, Johannes Gutenberg University of Mainz, Mainz, Germany.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Chatterjee
authors.forename: Judith
authors.initials: J
authors.lastname: Stegmüller
authors.forename: Philipp
authors.initials: P
authors.lastname: Schätzle
authors.forename: Khalad
authors.initials: K
authors.lastname: Karram
authors.forename: Michael
authors.initials: M
authors.lastname: Koroll
authors.forename: Hauke B
authors.initials: HB
authors.lastname: Werner
authors.forename: Klaus-Armin
authors.initials: KA
authors.lastname: Nave
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Trotter
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D009457:Neuroglia / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D053676:Syntenins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D011509:Proteoglycans; D034741:RNA, Small Interfering; D053676:Syntenins; D000099216:Chondroitin Sulfate Proteoglycan 4