title: Effect of surface characteristics on adherence of S. mutans biofilms to indirect resin composites.
abstract: The purpose of this study was to evaluate the adherence of biofilms to the surfaces of two indirect resin composites, Estenia C&B and Gradia. Slabs were prepared from the materials, and then either ground with 800-grit silicon carbide paper or polished with diamond pastes up to 1 microm. Artificial biofilms of Streptococcus mutans were grown on the composite slabs in an artificial mouth system for 20 hours. Thereafter, the amounts of retained biofilm on the surfaces were measured after sonication. Surface characteristics of the resins--such as surface roughness, amount of residual monomers, and distribution of filler particles--were examined. Two-way ANOVA revealed that the amount of retained biofilm varied (p<0.05) according to the composition and surface roughness of the material. In particular, biofilm adherence was lowest on Estenia C&B slabs when polished with diamond pastes up to 1 microm. It was thus concluded that the surface roughness and composition of a resin composite influenced biofilm adherence.
authors.affiliation: Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, 5-45 Yushima 1-chome, Bunkyo-ku, Tokyo 113-8549, Japan. ikeda.ope@tmd.ac.jp
authors.forename: Masaomi
authors.initials: M
authors.lastname: Ikeda
authors.forename: Khairul
authors.initials: K
authors.lastname: Matin
authors.forename: Toru
authors.initials: T
authors.lastname: Nikaido
authors.forename: Richard M
authors.initials: RM
authors.lastname: Foxton
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000704:Analysis of Variance; D001422:Bacterial Adhesion; D018441:Biofilms; D003188:Composite Resins / Q000737:chemistry; D003775:Dental Polishing; D017266:Dental Prosthesis / Q000382:microbiology; D007284:Inlays; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000502:physiology*; D013499:Surface Properties
chemical_list: D003188:Composite Resins; C470230:Gradia; D008689:Methacrylates; D011140:Polyurethanes; C091839:urethanetrimethacrylate