title: Mutagenic and cytotoxic properties of three herbal plants from Southeast Asia.
abstract: Three popular medicinal plants regarded as improving human sexual function in some parts of Southeast Asia were analysed for their mutagenic properties using modified Ames test (fluctuation test). Extract of one of the plants, Tacca integrifolia Ker-Gawl., was found to be mutagenic using Salmonella typhimurium strains TA98 and TA100. Extract of T. integrifolia, Eurycoma longifolia Jack and Helmintostachys zeylanica (L.) Hook were cytotoxic to human cell lines, Hep2 and HFL1, with IC50 ranging from 11 mug/ml to 55 mug/ml. Extract of E. longifolia was the most cytotoxic with IC50 of 11 mug/ml and 13 mug/ml on Hep2 and HFL1 cell lines respectively. Combined extract of T. integrifolia and H. zeylanica was more cytotoxic than single extract on both Hep2 and HFL1 cell lines while combined extract of E. longifolia and H. zeylanica was more cytotoxic than single extract on Hep2 cell lines. Under the conditions of this study it can be concluded that T. integrifolia is mutagenic and the combined extracts of the medicinal plants was highly cytotoxic.
authors.affiliation: Infectious Disease Research Centre, Institute for Medical Research, 50588 Kuala Lumpur, Malaysia. fuat@imr.gov.my
authors.forename: A R
authors.initials: AR
authors.lastname: Mohd-Fuat
authors.forename: E A
authors.initials: EA
authors.lastname: Kofi
authors.forename: G G C
authors.initials: GG
authors.lastname: Allan
mesh_terms: D001210:Asia, Southeastern; D002460:Cell Line / Q000187:drug effects; D035701:Eurycoma / Q000737:chemistry* / Q000145:classification; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
chemical_list: D009153:Mutagens; D010936:Plant Extracts