Apoptotic and necrotic influence of dental resin polymerization initiators in human gingival fibroblast cultures.
The aim of this study was to examine the apoptotic and necrotic influence of four dental resin polymerization initiators--namely benzoyl peroxide (BPO), camphorquinone (CQ), dimethylaminoethyl methacrylate (DMAEMA), and dimethyl-para-toluidine (DMPT)--on human gingival fibroblast (HGF) cells. To this end, the growth inhibition of HGF cells with 1 mM BPO, CQ, and DMAEMA, and 500 microM DMPT was evaluated using Cell Counting Kit-8. Then, cell cycle analysis by flow cytometry was used to assess propidium iodide-stained cells (distribution of cells in G0/G1, S, G2/M phases). All four dental resin polymerization initiators induced G0/G1 cell cycle arrest. As for the patterns of cell death (necrosis and/or apoptosis), they were analyzed using Annexin V-FITC/PI staining with flow cytometry. All four dental resin polymerization initiators most likely induced necrosis.
Masuki
Kouhei
K
Department of Biomaterials Science, Graduate School of Biomedical Science, Hiroshima University, Hiroshima, Japan.
Nomura
Yuji
Y
Bhawal
Ujjal Kumar
UK
Sawajiri
Masahiko
M
Hirata
Isao
I
Nahara
Yukinori
Y
Okazaki
Masayuki
M
D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D001585:Benzoyl Peroxide; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D003188:Composite Resins / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008689:Methacrylates; D009336:Necrosis; D044367:Phase Transition; D017319:Photosensitizing Agents / Q000633:toxicity*; D019163:Reducing Agents / Q000633:toxicity*; D013729:Terpenes; D014052:Toluidines
D017304:Annexin A5; D003188:Composite Resins; D005456:Fluorescent Dyes; D008689:Methacrylates; D017319:Photosensitizing Agents; D019163:Reducing Agents; D013729:Terpenes; D014052:Toluidines; C048339:camphoroquinone; D016650:Fluorescein-5-isothiocyanate; C049840:2-(dimethylamino)ethyl methacrylate; C015835:dimethyl-4-toluidine; D001585:Benzoyl Peroxide