Protective effect of green tea polyphenols on tributyltin-induced oxidative damage detected by in vivo and in vitro models.
The current study investigated the protective effects of green tea polyphenols (GTPP) on TBT-induced oxidative damage. The results showed that reactive oxygen species (ROS) production and malondialdehyde content of the liver in mice exposed to TBT were reduced in the GTPP-treated group compared to the untreated group. The intracellular ROS level was elevated in TBT-treated human FL cells in a time-dependent manner. Comet assay data demonstrated that the number of cells with damaged DNA in untreated mice was found to be significantly higher compared to GTPP-treated mice. Damage to the nuclei and mitochondria observed in TBT-treated mice were alleviated in mice treated with both TBT and GTPP. The results represent the first observation that GTPP were effective in reducing TBT-induced oxidative damage both in vivo and in vitro. The possible protective mechanism may be due to the powerful ability of GTPP to scavenge ROS and prevent DNA breaks. We conclude that GTPP could be an effective agent or food supplement to reduce the cytotoxicity of TBT.
Liu
Huigang
H
Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, Hangzhou, China.
Guo
Zonglou
Z
Xu
Lihong
L
Hsu
Stephen
S
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring / Q000379:methods*; D005419:Flavonoids / Q000008:administration & dosage*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008962:Models, Theoretical; D018384:Oxidative Stress; D010636:Phenols / Q000008:administration & dosage*; D008517:Phytotherapy; D059808:Polyphenols; D017382:Reactive Oxygen Species / Q000378:metabolism; D013662:Tea; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013662:Tea; D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin; D008315:Malondialdehyde