title: Detection of human parvovirus B19 in papillary thyroid carcinoma.
abstract: To evaluate whether parvovirus B19, a common human pathogen, was also involved in papillary thyroid carcinoma (PTC), 112 paraffin-embedded thyroid specimens of benign nodules, papillary, medullary and follicular carcinomas, and normal controls were examined for B19 DNA and capsid protein by nested PCR, in situ hybridisation (ISH) and immunohistochemistry (IHC). The expression of the nuclear factor-kappaB (NF-kappaB) was investigated by IHC. The results showed B19 DNA commonly exists in human thyroid tissues; however, there were significant differences between PTC group and normal controls, and between PTC and nonneoplastic adjacent tissues (P<0.001). The presence of viral DNA in PTC neoplastic epithelium was confirmed by laser-capture microdissection and sequencing of nested PCR products. B19 capsid protein in PTC group was significantly higher than that of all the control groups and nonneoplastic adjacent tissues (P<or=0.001). Compared with control groups, the activation of NF-kappaB in PTC group was significantly increased (P<or=0.02), except for medullary carcinomas, and the activation of NF-kappaB was correlated with the viral protein presence (P=0.002). Moreover, NF-kappaB was colocalised with B19 DNA in the neoplastic epithelium of PTC by double staining of IHC and ISH. These results indicate for the first time a possible role of B19 in pathogenesis of PTC.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Wang
authors.forename: W P
authors.initials: WP
authors.lastname: Zhang
authors.forename: H X
authors.initials: HX
authors.lastname: Liu
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.forename: W Q
authors.initials: WQ
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: F R
authors.initials: FR
authors.lastname: He
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: Q G
authors.initials: QG
authors.lastname: Yan
authors.forename: L W
authors.initials: LW
authors.lastname: Chen
authors.forename: G S
authors.initials: GS
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D036022:Capsid Proteins / Q000032:analysis; D002291:Carcinoma, Papillary / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000032:analysis; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D013959:Thyroid Diseases / Q000821:virology; D013964:Thyroid Neoplasms / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D016328:NF-kappa B; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19