Target-assembled exciplexes based on Scorpion oligonucleotides.
Scorpion probes, specific DNA probe sequences maintained in a hairpin-loop, can be modified to carry the components of an exciplex for use as a novel fluorescence-based method for specific detection of DNA. The exciplex partners (5'-pyrenyl and 3'-naphthalenyl) were attached to oligonucleotides via phosphoramidate links to terminal phosphate groups. Hybridization of the probe to a complementary target in a buffer containing trifluoroethanol produced an obvious fluorescence change from blue (pyrene locally excited state emission) to green (exciplex emission).
Gbaj
Abdul
A
Wolfson Centre for Rational Structure-Based Design of Molecular Diagnostics, School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester M13 9PT, UK.
Walsh
Lindsey
L
Rogert
Maria Candelaria
MC
Sardarian
Alireza
A
Bichenkova
Elena V
EV
Etchells
Laura L
LL
Whitcombe
David
D
Douglas
Kenneth T
KT
D015342:DNA Probes / Q000378:metabolism*; D005456:Fluorescent Dyes; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D013050:Spectrometry, Fluorescence
D015342:DNA Probes; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes