Solution structure of a double mutant of the carboxy-terminal dimerization domain of the HIV-1 capsid protein.
As in other retroviruses, the HIV-1 capsid (CA) protein is composed of two domains, the N-terminal domain (NTD) and the C-terminal domain (CTD), joined by a flexible linker. The dimerization of the CTD is thought to be a critical step in the assembly of the immature and mature viral capsids. The precise nature of the functional form of CTD dimerization interface has been a subject of considerable interest. Previously, the CTD dimer was thought to involve a face-to-face dimerization observed in the early crystallographic studies. Recently, the crystallographic structure for a domain-swapped CTD dimer has been determined. This dimer, with an entirely different interface that includes the major homology region (MHR) has been suggested as the functional form during the Gag assembly. The structure determination of the monomeric wt CTD of HIV-1 has not been possible because of the monomer-dimer equilibrium in solution. We report the NMR structure of the [W184A/M185A]-CTD mutant in its monomeric form. These mutations interfere with dimerization without abrogating the assembly activity of Gag and CA. The NMR structure shows some important differences compared to the CTD structure in the face-to-face dimer. Notably, the helix-2 is much shorter, and the kink seen in the crystal structure of the wt CTD in the face-to-face dimer is absent. These NMR studies suggest that dimerization-induced conformational changes may be present in the two crystal structures of the CTD dimers and also suggest a mechanism that can simultaneously accommodate both of the distinctly different dimer models playing functional roles during the Gag assembly of the immature capsids.
Wong
Hing C
HC
Comprehensive Cancer Center, The University of Alabama at Birmingham, Birmingham, Alabama 35294-2041, USA.
Shin
Ronald
R
Krishna
N Rama
NR
D019943:Amino Acid Substitution; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D019281:Dimerization; D015497:HIV-1; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012996:Solutions / Q000494:pharmacology
D036022:Capsid Proteins; D050505:Mutant Proteins; D011498:Protein Precursors; D011994:Recombinant Proteins; D012996:Solutions; C059767:p55 gag precursor protein, Human immunodeficiency virus 1