Letter: Lanthanum-calcium-manganate (La0.67Ca0.33MnO3) nanoparticle assisted affinity probes for MALDI MS analysis of proteins.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) has been used extensively for the analysis of -intact proteins and for proteomics. Proteins or proteomics analyses with low molecular mass range have some limitations because of background interference from the salt or surfactant ions. Lanthanum-calcium- manganate La(0.67)Ca(0.33)MnO(3) nanoparticles (LCMONPs) have been used as the matrix-assisting material after surface modification to reduce the salt or surfactant interference. The detectable mass range was tested in the range of 800-16,000 Da. The surface-modified LCMONPs have affinity with protein molecules through ionic or electrostatic interaction at pH 4.0. The nanoparticles mixed with proteins give a spectrum with a large signal-to-noise ratio for the analytes, and effectively eliminate the noise from interfering salt or surfactant ions. The results indicate that surface-modified La(0.67)Ca(0.33)MnO(3) nanoparticles can be used with MALDI matrix for proteins to eliminate the excessive noise of selective surfactants or salt ions from the MALDI spectrum.
Mandal
Santi M
SM
De
Debasis
D
Roy
Sanat K
SK
Ghosh
Ananta K
AK
Ram
Shanker
S
Das
Amit K
AK
D002118:Calcium / Q000737:chemistry*; D003902:Detergents; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D007811:Lanthanum / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D017830:Octoxynol; D010087:Oxides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003902:Detergents; D007202:Indicators and Reagents; D017895:Manganese Compounds; D010087:Oxides; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C027424:manganese oxide; D007811:Lanthanum; D017830:Octoxynol; D002118:Calcium