Development of drug resistance in the population of colon cancer cells under the effect of multifunctional protein YB-1.
The effects of YB-1 gene on the expression level of P-glycoprotein and drug resistance of tumor cells were studied in cultured HCT116 colon cancer cells. Transitory transfection of chimeric YB-1/GFP gene rendered HCT116 cells a selective advantage in a medium with vinblastine, which caused translocation of the chimeric protein into cell nuclei. This was paralleled by an increase in the expression of P-glycoprotein (multiple drug resistance protein).
Vaiman
A V
AV
Institute of Carcinogenesis, N. N. Blokhin National Cancer Research Center, Russian Academy of Medical Sciences, Moscow. vaiman@yandex.ru
Stromskaya
T P
TP
Rybalkina
E Yu
EY
Sorokin
A V
AV
Ovchinnikov
L P
LP
Stavrovskaya
A A
AA
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018432:Drug Resistance, Multiple / Q000187:drug effects / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology; D051840:Y-Box-Binding Protein 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D011993:Recombinant Fusion Proteins; D051840:Y-Box-Binding Protein 1; D049452:Green Fluorescent Proteins; D014747:Vinblastine