title: microParaflo biochip for nucleic acid and protein analysis.
abstract: We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using a microRNA detection chip, (2) protein binding and function analysis using epitope, kinase substrate, or phosphopeptide chips, and (3) protein-binding analysis using oligonucleotide chips. These diverse categories of customizable arrays are based on the same biochip platform featuring a significant amount of flexibility in the sequence design to suit a wide range of research needs. The protocols of the array applications play a critical role in obtaining high quality and reliable results. Given the comprehensive and complex nature of the array experiments, the details presented in this chapter is intended merely as a useful information source of reference or a starting point for many researchers who are interested in genome- or proteome-scale studies of proteins and nucleic acids and their interactions.
authors.affiliation: Department of Biology and Biochemistry, University of Houston, TX, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Ailing
authors.initials: A
authors.lastname: Hong
authors.forename: Nijing
authors.initials: N
authors.lastname: Sheng
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.forename: Anna
authors.initials: A
authors.lastname: Matejko
authors.forename: Kyu-Yeon
authors.initials: KY
authors.lastname: Jun
authors.forename: Onnop
authors.initials: O
authors.lastname: Srivannavit
authors.forename: Erdogan
authors.initials: E
authors.lastname: Gulari
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Gao
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Zhou
mesh_terms: D035683:MicroRNAs / Q000032:analysis; D046228:Microarray Analysis / Q000379:methods*; D044085:Microfluidics; D009696:Nucleic Acids / Q000032:analysis*; D010446:Peptide Fragments / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
chemical_list: D035683:MicroRNAs; D009696:Nucleic Acids; D010446:Peptide Fragments; D011506:Proteins