Isolation and partial characterization of A beta-glucuronidase of the mollusk Pomacea sp.
This paper studies the beta-glucuronidase in the mollusk Pomacea sp. The beta-glucuronidase was isolated 206-fold with a 1,5% yield and the cinetc parameters was: pH 5.0, 65 degrees C, K(m) of 72 x 10(-2) mM and molecular mass of 116 kDa. HPLC confirmed the purity. BaCl(2) increased beta-glucuronidase activity and SDS and NaH(2)PO(4) inhibited completely.
de Andrade
Maria Lúcia Lira
ML
Department of Biochemistry, Center for Biosciences, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
de Morais
Vanessa Duarte
VD
de Sousa Filho
João Felipe
JF
D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000378:metabolism; D049851:Gastropoda / Q000201:enzymology*; D005966:Glucuronidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D012995:Solubility; D013696:Temperature; D013997:Time Factors
D002809:Chondroitin Sulfates; D005966:Glucuronidase