title: Proteomic analysis of the pathophysiological process involved in the antisnake venom effect of Mucuna pruriens extract.
abstract: Previously, we reported the antisnake venom properties of a Mucuna pruriens seed extract (MPE) and tested its in vivo efficacy against Echis carinatus venom (EV) in short- (1 injection) and long-term (three weekly injections) treatments. The aim of the present study was to investigate plasma proteome changes associated with MPE treatments and identify proteins responsible for survival of envenomated mice (CHALLENGED mice). Six treatment groups were studied. Three control groups: one saline, one short-term and one long-term MPE treatment. One group received EV alone. Two test groups received EV with either a short-term or long-term MPE treatment (CHALLENGED mice). The plasma from each group was analysed by 2-DE/MALDI-TOF MS. The most significant changes with treatment were: albumin, haptoglobin, fibrinogen, serum amyloid A and serum amyloid P. Most of these changes were explained by EV effects on coagulation, inflammation and haemolysis. However, MPE treatments prevented the EV-induced elevation in HPT. Consequently, HPT levels were similar to controls in the plasma of CHALLENGED mice. The plasma of CHALLENGED mice showed substantial proteomic modifications. This suggests the mechanism of MPE protection involves the activation of counterbalancing processes to compensate for the imbalances caused by EV.
authors.affiliation: Department of Internal Medicine, Endocrine-Metabolic Sciences and Biochemistry, University of Siena, Via A. Moro 2, Siena, Italy. guerranti@unisi.it
authors.forename: Roberto
authors.initials: R
authors.lastname: Guerranti
authors.forename: Ifeanyi G
authors.initials: IG
authors.lastname: Ogueli
authors.forename: Erica
authors.initials: E
authors.lastname: Bertocci
authors.forename: Chiara
authors.initials: C
authors.lastname: Muzzi
authors.forename: John C
authors.initials: JC
authors.lastname: Aguiyi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cianti
authors.forename: Alessandro
authors.initials: A
authors.lastname: Armini
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.forename: Roberto
authors.initials: R
authors.lastname: Leoncini
authors.forename: Enrico
authors.initials: E
authors.lastname: Marinello
authors.forename: Roberto
authors.initials: R
authors.lastname: Pagani
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D031299:Mucuna / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D040901:Proteomics; D012639:Seeds / Q000737:chemistry; D014757:Viper Venoms / Q000037:antagonists & inhibitors*; D017819:Viperidae
chemical_list: D001798:Blood Proteins; D010936:Plant Extracts; D014757:Viper Venoms