title: Low-temperature improved-throughput method for analysis of brain fatty acids and assessment of their post-mortem stability.
abstract: Deficiency of docosahexaenoic acid (DHA) and other omega-3 (omega3) fatty acids may constitute an alterable risk factor for Alzheimer's disease (AD). Mechanisms of potential involvement of DHA in the disease process have been postulated primarily from studies in vitro and in mouse models of AD. Information on the fatty acid profile of the brain in AD itself is limited and in some respects contradictory. Interpretation of the findings is complicated by the diversity of methods used in previous studies and a lack of information as to the effect of post-mortem delay on the results. Here we report the development of a simple and highly reproducible method that enables relatively high-throughput measurement of the fatty acid composition in samples of brain tissue and using this method we have demonstrated that there is no significant change in fatty acid composition under conditions designed to model post-mortem delay of up to 3 days at 4 degrees C (or even at room temperature). The development of this method and the observation that delay of up to 3 days has no effect on fatty acid content will facilitate further studies of fatty acid composition on large cohorts of post-mortem brains.
authors.affiliation: Dementia Research Group, Institute of Clinical Neurosciences, Clinical Science at North Bristol, University of Bristol, Frenchay Hospital, Frenchay, Bristol BS16 1LE, UK. t.c.m.fraser@bristol.ac.uk
authors.forename: Thomas
authors.initials: T
authors.lastname: Fraser
authors.forename: Hannah
authors.initials: H
authors.lastname: Tayler
authors.forename: Seth
authors.initials: S
authors.lastname: Love
mesh_terms: D001681:Biological Assay / Q000295:instrumentation / Q000379:methods; D001831:Body Temperature / Q000502:physiology*; D001923:Brain Chemistry / Q000502:physiology*; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism / Q000502:physiology*; D008745:Methylation; D009448:Neurochemistry / Q000295:instrumentation / Q000379:methods*; D011180:Postmortem Changes; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D004281:Docosahexaenoic Acids