title: Improved leucoreduction of red blood cell units prepared after a 24-h hold with the platelet-rich plasma method using newly developed filters.
abstract: OBJECTIVE
  A previous study indicated that the extension of whole blood (WB) storage from 8 to 24 h at 20-24 degrees C before the processing of platelet-rich plasma (PRP)-depleted red blood cell (RBC) units had a negative effect on the efficacy of leucoreduction filters. In this study, we further characterized the phenomenon and tested the leucoreduction capacity of two newly developed filters.
  
  
  METHODS
  Whole blood was stored at 20-24 degrees C and processed at 4-h intervals between 8 and 24 h postcollection. Components were leucoreduced before storage. Efficacy of novel filters to leucoreduce 24-h-hold PRP-depleted RBC units was also evaluated.
  
  
  RESULTS
  Using a conventional filter, the mean residual white blood cell (WBC) counts in leucoreduced PRP-depleted RBCs were comparable in units prepared within 12 h from collection but gradually increased upon extended preprocessing storage from 0.36 +/- 0.03 at 12 h to 0.46 +/- 0.21, 0.76 +/- 0.54 and 1.72 +/- 1.76 x 10(6) per unit at 16, 20 and 24 h, respectively. However, the mean residual WBC content in 24-h-hold RBCs was reduced to 0.60 +/- 0.39 x 10(6) and 0.46 +/- 0.13 x 10(6) per units using RC2D and the prototypes B-1582 rev B filters, respectively.
  
  
  CONCLUSIONS
  For PRP-depleted RBC units, the extension of the WB room temperature storage from 8 to 24 h before processing is likely to require the introduction of newly developed filters having an increased leucoreduction capacity in order to meet the maximal residual WBC guideline in the RBCs.
authors.affiliation: Héma-Québec, R&D, avenue des Sciences-de-la-Vie, Québec, QC, Canada G1V 5C3. louis.thibault@hema-quebec.qc.ca
authors.forename: L
authors.initials: L
authors.lastname: Thibault
authors.forename: A
authors.initials: A
authors.lastname: Beauséjour
authors.forename: A
authors.initials: A
authors.lastname: Jacques
authors.forename: M J
authors.initials: MJ
authors.lastname: de Grandmont
authors.forename: R
authors.initials: R
authors.lastname: Lemieux
authors.forename: Y
authors.initials: Y
authors.lastname: Grégoire
authors.forename: E
authors.initials: E
authors.lastname: Ducas
authors.forename: G
authors.initials: G
authors.lastname: Boucher
mesh_terms: D002170:Canada; D017707:Erythrocyte Transfusion; D005374:Filtration / Q000295:instrumentation*; D006801:Humans; D047589:Leukocyte Reduction Procedures / Q000295:instrumentation* / Q000379:methods*; D053657:Platelet-Rich Plasma / Q000166:cytology; D013048:Specimen Handling; D013696:Temperature