title: A common polymorphism G-50T in cytochrome P450 2J2 gene is associated with increased risk of essential hypertension in a Russian population.
abstract: The present study was designed to test whether common polymorphism G-50T within the promoter of human CYP2J2 gene is associated with increased risk of essential hypertension in a Russian population. We studied 576 unrelated subjects, including 295 patients with hypertension and 281 healthy subjects. Genotyping for polymorphism G-50T of the CYP2J2 gene was performed by polymerase chain reaction and restriction fragment length polymorphism techniques. The frequency of a -50T variant allele of CYP2J2 gene was significantly higher in patients with hypertension versus healthy controls (OR 4.03 95%CI 1.80-9.04 p=0.0004). The association of a -50GT genotype with hypertension remained significant after adjustment for age, gender and family history of hypertension by multivariate logistic regression (OR 4.78 95%CI 1.87-12.27 p=0.001). It has been found that OR for -50GT genotype x gender interaction (OR 4.48 95%CI 1.93-10.39 p=0.00048) was slightly higher than OR for -50GT genotype (OR 4.43 95%CI 1.91-10.29 p=0.00052), suggesting a weak effect of gender on the risk of hypertension in the heterozygous carriers of -50GT genotype. A family history of hypertension has no effect on the association between a -50GT genotype and hypertension. In present study we demonstrate for the first time that a CYP2J2*7 allele of the CYP2J2 gene is clearly associated with an increased risk of essential hypertension. Furthermore, this study highlights the importance of P-450 epoxygenase pathway of arachidonic acid metabolism in the pathogenesis of hypertensive disease.
authors.affiliation: Department of Biology, Medical Genetics and Ecology, Kursk State Medical University, Kursk, Russia. polonikov@rambler.ru
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Polonikov
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Ivanov
authors.forename: Maria A
authors.initials: MA
authors.lastname: Solodilova
authors.forename: Irina V
authors.initials: IV
authors.lastname: Khoroshaya
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Kozhuhov
authors.forename: Vladimir E
authors.initials: VE
authors.lastname: Ivakin
authors.forename: Ludmila N
authors.initials: LN
authors.lastname: Katargina
authors.forename: Ol'ga E
authors.initials: OE
authors.lastname: Kolesnikova
mesh_terms: D000328:Adult; D016718:Arachidonic Acid / Q000378:metabolism; D000090762:Cytochrome P-450 CYP2J2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000209:etiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010105:Oxygenases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012727:Sex Characteristics
chemical_list: C000717350:CYP2J2 protein, human; D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D000090762:Cytochrome P-450 CYP2J2