title: Inhibition of influenza M2-induced cell death alleviates its negative contribution to vaccination efficiency.
abstract: The effectiveness of recombinant vaccines encoding full-length M2 protein of influenza virus or its ectodomain (M2e) have previously been tested in a number of models with varying degrees of success. Recently, we reported a strong cytotoxic effect exhibited by M2 on mammalian cells in vitro. Here we demonstrated a decrease in protection when M2 was added to a DNA vaccination regimen that included influenza NP. Furthermore, we have constructed several fusion proteins of conserved genes of influenza virus and tested their expression in vitro and protective potential in vivo. The four-partite NP-M1-M2-NS1 fusion antigen that has M2 sequence engineered in the middle part of the composite protein was shown to not be cytotoxic in vitro. A three-partite fusion protein (consisting of NP, M1 and NS1) was expressed much more efficiently than the four-partite protein. Both of these constructs provided statistically significant protection upon DNA vaccination, with construct NP-M1-M2-NS1 being the most effective. We conclude that incorporation of M2 into a vaccination regimen may be beneficial only when its apparent cytotoxicity-linked negative effects are neutralized. The possible significance of this data for influenza vaccination regimens and preparations is discussed.
authors.affiliation: Cure Lab, Canton, Massachusetts, United States of America. ilyinskii@curelab.com
authors.forename: Petr O
authors.initials: PO
authors.lastname: Ilyinskii
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Gambaryan
authors.forename: Anatoli B
authors.initials: AB
authors.lastname: Meriin
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gabai
authors.forename: Alex
authors.initials: A
authors.lastname: Kartashov
authors.forename: Galini
authors.initials: G
authors.lastname: Thoidis
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Shneider
mesh_terms: D000818:Animals; D015153:Blotting, Western; D016923:Cell Death / Q000502:physiology*; D002460:Cell Line; D007252:Influenza Vaccines / Q000276:immunology*; D009975:Orthomyxoviridae / Q000502:physiology*; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000473:pathology; D010957:Plasmids
chemical_list: D007252:Influenza Vaccines