microParaflo biochip for nucleic acid and protein analysis.
We describe in this chapter the use of oligonucleotide or peptide microarrays (arrays) based on microfluidic chips. Specifically, three major applications are presented: (1) microRNA/small RNA detection using a microRNA detection chip, (2) protein binding and function analysis using epitope, kinase substrate, or phosphopeptide chips, and (3) protein-binding analysis using oligonucleotide chips. These diverse categories of customizable arrays are based on the same biochip platform featuring a significant amount of flexibility in the sequence design to suit a wide range of research needs. The protocols of the array applications play a critical role in obtaining high quality and reliable results. Given the comprehensive and complex nature of the array experiments, the details presented in this chapter is intended merely as a useful information source of reference or a starting point for many researchers who are interested in genome- or proteome-scale studies of proteins and nucleic acids and their interactions.
Zhu
Qi
Q
Department of Biology and Biochemistry, University of Houston, TX, USA.
Hong
Ailing
A
Sheng
Nijing
N
Zhang
Xiaolin
X
Matejko
Anna
A
Jun
Kyu-Yeon
KY
Srivannavit
Onnop
O
Gulari
Erdogan
E
Gao
Xiaolian
X
Zhou
Xiaochuan
X
D035683:MicroRNAs / Q000032:analysis; D046228:Microarray Analysis / Q000379:methods*; D044085:Microfluidics; D009696:Nucleic Acids / Q000032:analysis*; D010446:Peptide Fragments / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
D035683:MicroRNAs; D009696:Nucleic Acids; D010446:Peptide Fragments; D011506:Proteins