Sequence and phylogenetic analysis of the S-class genome segments of a duck orthoreovirus.
In spite of common properties duck orthoreoviruses (DRVs) are antigenically different from other avian orthoreoviruses (ARVs). We analyzed the S-class genome segments of the DRV S12 and compared them with S-class genome segments of other orthoreoviruses compared to those of ARV S1133. The full-length S-class sigmaA, sigmaB, sigmaNS, and sigmaC genes were determined and compared with other ARVs to study the degree of genetic divergence and evolution of DRVs. The alignment of the DRV S12 sigmaA, sigmaB, sigmaNS, and sigmaC genes with DRV 89026 showed 90.0%, 93.6%, 88.0%, and 93.1% nucleotide identity and 97.1%, 94.3%, 95.8%, and 93.7% amino acid identity, respectively. The alignment of the DRV S12 sigmaA, sigmaB, sigmaNS, and sigmaC genes with other ARVs revealed 76.0-77.1%, 52.5-55.1%, 78.4-79.6%, and 2.7-9.9% nucleotide identity and 89.5-91.2%, 61.4-62.0%, 91.6-92.7% and 22.6-26.7% amino acid identity, respectively. Phylogenetic analyses demonstrated that DRVs were quite different from other ARVs and provided the evidence for the diversity among avian orthoreoviruses.
Zhang
Y
Y
Avian Infectious Disease Division, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, PR China. yunzhang03@yahoo.com
Liu
M
M
Qu
L
L
Xiang
W
W
Guo
D
D
Yuan
X
X
Ge
M
M
Zhang
C
C
D000595:Amino Acid Sequence; D000818:Animals; D036022:Capsid Proteins / Q000235:genetics; D004372:Ducks; D016679:Genome, Viral; D008969:Molecular Sequence Data; D030101:Orthoreovirus, Avian / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics; D012088:Reoviridae Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014758:Viral Core Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics
D036022:Capsid Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; C031728:sigma 1 protein, reovirus; C053179:sigma protein 3, Reovirus; C403292:sigmaA protein, avian reovirus