title: Microbacterium ginsengisoli sp. nov., a beta-glucosidase-producing bacterium isolated from soil of a ginseng field.
abstract: Strain Gsoil 259(T), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the Republic of Korea and characterized in order to determine its taxonomic position. Cells were Gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16S rRNA gene sequence analysis revealed that strain Gsoil 259(T) belonged to the genus Microbacterium and was closely related to Microbacterium arborescens IFO 3750(T) (98.5 %) and Microbacterium imperiale IFO 12610(T) (97.9 %). However, it has low values for DNA-DNA relatedness with the above strains (20.7 and 17.5 %, respectively). Strain Gsoil 259(T) possessed chemotaxonomic markers that were consistent with classification in the genus Microbacterium, i.e. MK-11 and MK-12 were the major menaquinones and anteiso-C(17 : 0), anteiso-C(15 : 0) and iso-C(16 : 0) were the predominant cellular fatty acids. The DNA G+C content was 69.4 mol%. The cell-wall sugar was rhamnose and the diamino acid in the cell-wall peptidoglycan was ornithine. On the basis of data from this polyphasic study, strain Gsoil 259(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium ginsengisoli sp. nov. is proposed. The type strain is Gsoil 259(T) (=KCTC 19189(T) =DSM 18659(T)).
authors.affiliation: Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Min-Ju
authors.initials: MJ
authors.lastname: Park
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
authors.forename: Ho-Bin
authors.initials: HB
authors.lastname: Kim
authors.forename: Wan-Taek
authors.initials: WT
authors.lastname: Im
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Nak-Kyun
authors.initials: NK
authors.lastname: Soung
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D005894:Panax / Q000254:growth & development*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D001617:beta-Glucosidase