Chronic atrial fibrillation: decreased actin expression in patients with chronic atrial fibrillation.
OBJECTIVE
Chronic atrial fibrillation (AF) is characterized by a severe contractile dysfunction and myolysis, which develops progressively. Myolysis and the remodelling of the cellular ultrastructure is associated with the replacement of sarcomeres by glycogen. The aim of our study was to determine if myolysis is characterized by a reduction in actin concentration.


METHODS
Right atrial samples from 18 patients undergoing elective cardiac surgery were excised and immediately frozen in liquid nitrogen. Eight patients had chronic AF (>3 months) and 10 patients were in sinus rhythm (SR). Actin concentration was determined by sodium dodecyl sulfate-polycrylamide gel electrophoresis (SDS-PAGE), Western blot and quantified by optical densitometry.


RESULTS
Immunoblot analysis demonstrated actin expression in all hearts. In myocardial samples from patients with chronic AF we found a 2.1 fold reduction in actin expression. (P<0.001)


CONCLUSIONS
The decrease in actin concentration via myolysis, might decrease energy consumption and be an additional mechanism for contractile dysfunction in chronic AF.
Schäfler
A E
AE
Department of Cardiac Surgery, University of Ulm, Ulm, Germany. aschaefler@gmx.de
Friedl
R
R
Hekmat
K
K
Hannekum
A
A
Kirmanoglou
K
K
D000199:Actins / Q000032:analysis*; D000368:Aged; D001281:Atrial Fibrillation / Q000378:metabolism*; D002908:Chronic Disease; D015536:Down-Regulation; D005260:Female; D006325:Heart Atria / Q000737:chemistry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000737:chemistry*
D000199:Actins