title: Effect of local neutralization of basic fibroblast growth factor or vascular endothelial growth factor by a specific antibody on the development of the corpus luteum in the cow.
abstract: Active angiogenesis and progesterone (P) synthesis occur in parallel during development of the corpus luteum (CL). Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) are known to stimulate angiogenesis and P synthesis in vitro. The aim of the present study was to investigate the impact of bFGF or VEGF on the CL development in the cow by using a specific antibody against bFGF or VEGF. bFGF antibody, VEGF antibody, or saline as a control (n = 4 cows/treatment) were injected directly into the CL immediately after ovulation (Day 1), and the treatment was continued for 3 times/day over 7 days. Luteal biopsies were applied on Day 8 of the estrous cycle to determine the expression of genes associated with P synthesis and angiogenesis. Intraluteal injections with the bFGF antibody or the VEGF antibody markedly decreased the CL volume, plasma P concentration and StAR mRNA expression. bFGF antibody treatment decreased the mRNA expression of bFGF, FGF receptor-1, VEGF120, and angiopoietin (ANPT)-1, and increased ANPT-2/ANPT-1 ratio. However, VEGF antibody treatment decreased ANPT-2 mRNA expression and ANPT-2/ANPT-1 ratio. These results indicate that local neutralization of bFGF or VEGF changes genes regulating angiogenesis and P synthesis, and remarkably suppresses the CL size and P secretion during the development of CL in the cow, supporting the concept that bFGF and VEGF control the CL formation and function.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yamashita
authors.forename: Daichi
authors.initials: D
authors.lastname: Kamada
authors.forename: Koumei
authors.initials: K
authors.lastname: Shirasuna
authors.forename: Motozumi
authors.initials: M
authors.lastname: Matsui
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kida
authors.forename: Bajram
authors.initials: B
authors.lastname: Berisha
authors.forename: Dieter
authors.initials: D
authors.lastname: Schams
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
mesh_terms: D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism; D042702:Angiopoietin-2 / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D030762:Estrous Cycle / Q000097:blood / Q000187:drug effects / Q000235:genetics; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000097:blood; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology / Q000502:physiology; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D000906:Antibodies; D010750:Phosphoproteins; D042461:Vascular Endothelial Growth Factor A; D000098682:Steroidogenic Acute Regulatory Protein; D016222:Fibroblast Growth Factor 2; D011374:Progesterone; D051497:Receptor, Fibroblast Growth Factor, Type 2