Effects of fumonisin B(1) on the expression of cytokines and chemokines in human dendritic cells.
Fumonisin B(1) (FB(1)) is a mycotoxin produced by the fungus Fusarium verticillioides, which commonly infects corn and other crops across the world. Exposure to FB(1) is known to have toxic and carcinogenic effects in animals, and to express toxicity in cells. In this study, we investigated mechanisms whereby FB(1) may induce immunotoxic effects in human dendritic cells (DC) differentiated from human peripheral blood mononuclear cells. mRNA and protein levels of a number of cytokines and chemokines were analyzed in DC, after exposure to 100 microM FB(1), 10 ng/ml LPS, or a combination of 100 microM FB(1) and 10 ng/ml LPS for 6h or 24h. Exposure to FB(1) resulted in an increase in the expression of IFNgamma and CXCL9. Moreover, FB(1) inhibited the LPS-induced expression of IL-6, IL-1beta, CCL3 and CCL5. The other cytokines studied (TNFalpha, IL-12, IL-18 and IL-23) were not affected by FB(1) in DC. The results of this study indicate that FB(1) has an impact on the expression of cytokines and chemokines in human DC, and in addition to its other toxic effects, FB(1) may also be potentially immunotoxic to humans.
Stockmann-Juvala
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H
Unit of Excellence for Immunotoxicology, Finnish Institute of Occupational Health, Helsinki, Finland. stockmannjuvala@yahoo.com
Alenius
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H
Savolainen
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K
D002274:Carcinogens, Environmental / Q000633:toxicity*; D002478:Cells, Cultured; D054370:Chemokine CXCL9 / Q000096:biosynthesis; D018925:Chemokines / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D004781:Environmental Exposure; D004797:Enzyme-Linked Immunosorbent Assay; D037341:Fumonisins / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000633:toxicity; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D002274:Carcinogens, Environmental; D054370:Chemokine CXCL9; D018925:Chemokines; D016207:Cytokines; D018076:DNA, Complementary; D037341:Fumonisins; D007378:Interleukins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C056933:fumonisin B1; D012313:RNA; D007371:Interferon-gamma