Comparison of cryopreserved human sperm in vapor and liquid phases of liquid nitrogen: effect on motility parameters, morphology, and sperm function.
OBJECTIVE
To compare the effects of cryopreserved sperm in vapor and liquid phases of liquid nitrogen on sperm motility, morphology, and sperm function.


METHODS
Experimental study.


METHODS
Andrology laboratory at Ramathibodi Hospital, Thailand.


METHODS
Thirty-eight semen samples with normal motility and sperm count were collected from 38 men who were either patients of an infertility clinic or had donated sperm for research.


METHODS
Each semen sample was divided into two aliquots. Samples were frozen with static-phase vapor cooling. One aliquot was plunged into liquid nitrogen (-196 degrees C), and the other was stored in vapor-phase nitrogen (-179 degrees C) for 3 days. Thawing was performed at room temperature.


METHODS
Motility was determined by using computer-assisted semen analysis, sperm morphology was determined by using eosin-methylene blue staining, and sperm function was determined by using a hemizona binding test.


RESULTS
Most of the motility parameters of sperm stored in the vapor phase were not significantly different from those stored in the liquid phase of liquid nitrogen, except in amplitude of lateral head displacement. The percentages of normal sperm morphology in both vapor and liquid phases also were not significantly different. There was no significant difference in the number of bound sperm in hemizona between sperm cryopreserved in both vapor and liquid phases of liquid nitrogen.


CONCLUSIONS
Cryopreservation of human sperm in a vapor phase of liquid nitrogen was comparable to cryopreservation in a liquid phase of liquid nitrogen.
Punyatanasakchai
Piyaphan
P
Department of Obstetrics and Gynaecology, Faculty of Medicine, Ramathibodhi Hospital, Mahidol University, Bangkok, Thailand. drpiyaphan@hotmail.com
Sophonsritsuk
Areephan
A
Weerakiet
Sawaek
S
Wansumrit
Surapee
S
Chompurat
Deonthip
D
D048430:Cell Shape; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D006801:Humans; D008297:Male; D009584:Nitrogen; D012662:Semen Preservation; D013048:Specimen Handling; D013076:Sperm Count; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology*; D014835:Volatilization
D003451:Cryoprotective Agents; D009584:Nitrogen