title: Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
abstract: Although IL-3 is commonly used for culture of human progenitor-derived mast cells together with Stem cell factor (SCF) and IL-6, the effect of IL-3 on human mast cell differentiation has not been well elucidated. Human bone marrow CD34+ progenitors were cultured for up to 12 weeks in the presence of rhSCF and rhIL-6 either with rhIL-3 (IL-3 (+)) or without rhIL-3 (IL-3 (-)) for the initial 1-week of culture. Total cell number increased at 2 weeks in IL-3 (+), as compared to IL-3 (-), but changes in the appearance of mast cells were delayed. When IL-3 was present for the initial 1-week culture, granules looked more mature with IL-3 than without IL-3. However, tryptase and chymase contents, and surface antigen expression (CD18, CD51, CD54, and CD117) were not altered by IL-3. Surface expression and mRNA level of FcepsilonRIalpha and histamine release by crosslinking of FcepsilonRIalpha did not differ from one preparation to the next. GeneChip analysis revealed that no significant differences were observed between IL-3 (+) and IL-3 (-) cells either when inactivated or activated by aggregation of FcepsilonRIalpha. These findings indicate that initial incubation of human bone marrow CD34+ progenitors with IL-3 does not affect the differentiation of mast cells.
authors.affiliation: Department of Respiratory Medicine, National Hospital Organization, Takasaki Hospital, Takasaki, Japan. yshimizu@takasaki-hosp.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Okayama
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sakai
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Maeno
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suga
authors.forename: Toru
authors.initials: T
authors.lastname: Miura
authors.forename: Shinji
authors.initials: S
authors.lastname: Takai
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kurabayashi
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Saito
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects*; D020411:Oligonucleotide Array Sequence Analysis; D019089:Stem Cell Factor / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology
chemical_list: D018952:Antigens, CD34; D007377:Interleukin-3; D015850:Interleukin-6; D019089:Stem Cell Factor