title: Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells.
abstract: Epigenetic regulation including DNA methylation plays an important role in several differentiation processes. We profiled global DNA methylation in the neural differentiation of P19 embryonic carcinoma cells using a microarray-based method called MIAMI. We found a genome-wide demethylation of genes. This suggests demethylation rather than methylation is important in neural differentiation.
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Japan. ihatada@showa.gunma-u.ac.jp.
authors.forename: Izuho
authors.initials: I
authors.lastname: Hatada
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Japan.
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Morita
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Horii
authors.affiliation: Laboratory of Functional Analysis in silico, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
authors.forename: Riu
authors.initials: R
authors.lastname: Yamashita
authors.affiliation: Laboratory of Functional Analysis in silico, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Nakai
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D019175:DNA Methylation; D054278:Embryonal Carcinoma Stem Cells / Q000473:pathology*; D020869:Gene Expression Profiling; D016678:Genome / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger