Comparative study of P19 EC stem cell differentiation in between conventional hanging drop and the zebrafish chorion as a bio-derived material.
Various materials including glass and polymers have been widely used for stem cell culture due to their biocompatibility. However, the roles of these materials are fundamentally limited because they cannot realize or imitate the complex biological functions of living tissues, except in very simple cases. Here, the development of a bio-derived material suitable for stem cell culture and improvement of differentiation efficiency to specific cell lineages with no stimulating agents by using a chorion obtained from a fertilized zebrafish egg through the removal of the yolk and embryonic cell mass from the egg is reported. Mouse P19 EC stem cells introduced into the empty chorion form a uniform embryoid body (EB) without addition of any inducing agent. It is demonstrated that the zebrafish chorion with nanopores improves efficiencies greatly in the EB formation, cell proliferation, and lineage-specific differentiations compared to those of the conventional hanging drop culture method.
Dae Seok Na
Optoelectronic Materials Research Laboratory Korea Institute of Science Technology, Seoul 136-791, Korea.
Lee
Hwang
H
Sun Uk Kim
Chang Nam Hwang
Sang Ho Lee
Ji Yoon Kang
Jai Kyeong Kim
James Jungho Pak
D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002823:Chorion; D005260:Female; D008422:Materials Testing; D051379:Mice; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology
D001672:Biocompatible Materials