title: Effects of the insecticides malathion and diazinon on the early oogenesis in mice in vitro.
abstract: Malathion and diazinon are two of the most commonly used organophosphorous (OP) agrochemicals. Several studies show that these pesticides exert several effects on mammalian spermatogenesis. Nevertheless, there are no studies concerning their effects on oogenesis. The objective of this study was to evaluate the effects of these insecticides on the viability of in vitro cultured mouse oocytes during the early oogenesis and to get a further understanding of the molecular mechanisms by which OP insecticides act and affect germinal cells. Oocytes were cultured from fetal ovaries for 10 days, when most oocytes had reached the diplotene stage (germinal vesicle stage). Cultures were exposed to different concentrations of malathion or diazinon for 24 h, and the effect on oocyte viability was assessed. Gene expression in oocytes exposed to the insecticides was analyzed by generating cDNA libraries and performing differential screenings. Results show a significant decrease in oocytes survival after 24-h exposure to 250 microM malathion or 900 nM diazinon, and the effect of these insecticides on the regulation of genes encoding proteins involved in transcription (BP75), translation (ribosomal protein S5), and mitochondrial function (cytochrome oxidase subunits I and III), providing evidence for OP insecticides as toxicants for mammals oocytes during the early oogenesis.
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, CP 09340, DF. México.
authors.forename: Edmundo
authors.initials: E
authors.lastname: Bonilla
authors.forename: Fidel
authors.initials: F
authors.lastname: Hernández
authors.forename: Leticia
authors.initials: L
authors.lastname: Cortés
authors.forename: Miriam
authors.initials: M
authors.lastname: Mendoza
authors.forename: Joel
authors.initials: J
authors.lastname: Mejía
authors.forename: Edgar
authors.initials: E
authors.lastname: Carrillo
authors.forename: Eduardo
authors.initials: E
authors.lastname: Casas
authors.forename: Miguel
authors.initials: M
authors.lastname: Betancourt
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002470:Cell Survival / Q000187:drug effects; D003976:Diazinon / Q000633:toxicity*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D015723:Gene Library; D066298:In Vitro Techniques; D007306:Insecticides / Q000633:toxicity*; D008294:Malathion / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D009866:Oogenesis / Q000187:drug effects*; D010053:Ovary / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D007306:Insecticides; D008294:Malathion; D003976:Diazinon