Taxonomic status of the agent of cereals pale-green dwarf and proposition of founding in the class of Mollicutes, of the order III Acholeplasmatales, family I Acholeplasmataceae, genus II Pluraplasma gen. nov., and its first species Pluraplasma granulum sp. nov.
The paper includes the data concerning the taxonomic status of the agent of cereals pale-green dwarf (ACPGD) which has been defined as the phytopathogenic variant of the mollicute Acholeplasma laidlawii and called A. laidlawii var. granulum. Since besides phytopathogenicity ACPGD has such fundamental differences from A.laidlawii as: a very large genome of 2200 thousand pairs of nucleotides (t.p.n.) to 2310 t.p.n. that practically equals a sum of genomes of A. laidlawii (1600 t.p.n.) and phytoplasmas (710 t.p.n.); two forms of DNA-dependent RNA-polymerase (one form functions in A. laidlawii); a capacity to form extracellular fructosobisphos-phatase which looks like its hypothetical phylogenetic precursor a bacteria Bacillus subtilis; availability of numerous fermentative activities which are absent in acholeplasmas; peculiar relation to sterols availability in nutrient media that is not characteristic of all the known acholeplasmas; extremely rich, as to quantity and quality, composition of antigens to react almost homologously with antibodies to representatives of Acholeplasma genus and separate species of Mycoplasma genus; great similarity (above 88 %) of sequences of 16S rRNA of ACPGD and representatives of Phytoplasma genus and other properties described in the paper, so it is concluded, that proceeding from its characteristics, ACPGD cannot be referred to either of the existing genera of the Mollicules class, because according to all its features this mollicute is a transition form of the microorganism, it is the hitherto unknown chain between these genera of mollicutes and sporiferous bacteria, ACPGD is a probable representative of mollicute precursor with genome of about 1600 t.p.n. and, first of all, of genera Acholeplasma and Phytoplasma which arised as a result of the evolutionary splitting of its genome. On this basis, it is recommended to found for ACPGD in the Mollicutes class, order III Acholeplasmatales, family I Acholeplasmataceae, a new genus II Pluraplasma gen. nov. and its first species Pluraplasma granulum sp. nov., the strain 118 being its typical representative.
Skripal
I G
IG
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Yegorov
O V
OV
D000128:Acholeplasma laidlawii / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D000595:Amino Acid Sequence; D001483:Base Sequence; D018556:Crops, Agricultural / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S