Prevalence and abundance of uncultivated Megasphaera-like bacteria in the human vaginal environment.
Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests that both phylotypes are present in higher concentrations in women with bacterial vaginosis.
Zozaya-Hinchliffe
Marcela
M
The Research Institute for Children,1 New Orleans, Louisiana, USA.
Martin
David H
DH
Ferris
Michael J
MJ
D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D008145:Louisiana / Q000453:epidemiology; D045851:Megasphaera / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000453:epidemiology* / Q000382:microbiology*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S