[Protective effect of Ophiopogonis polysaccharide MDG-1 on experimental myocardial ischemic rats].
OBJECTIVE
To investigate the protective effect of Ophiopogonis polysaccharide (MDG-1) on isolated myocardium ischemia/reperfusion injury (IRI) and subcutaneous injection of isoprenaline induced acute myocardial ischemia.


METHODS
In ex vivo heart experiment: Langendorff guinea pigs were randomly divided into the IRI group, the fructose sodium diphosphate (FDP) group, treated with FDP 10(-6) - 10(-4) g/mL for positive control and the MDG groups treated with MDG-1 10(-6) - 10(-4) g/mL. The amplitude and frequency of cardiac contraction, coronary blood flow at different time points after ischemia reperfusion were measured. In integral animal experiments: acute myocardium ischemia model rats established by subcutaneous injection of isoprenaline were used, they were administered with MDG-1 in dosage of 10, 20 and 40 mg/kg respectively, and controlled with propranolol. Besides, a normal control group and an untreated model group for control were set up. The ST segment shift in ECG and lactate dehydrogenase (LDH) activity in serum were observed.


RESULTS
Ex vivo heart experiment showed that different doses of MDG-1 can increase IRI caused abnormal coronary blood flow, quickly resume the heart contraction and restrain the quickened heart rate (all P < 0.01). The integral animal experiment showed that oral administration of 40 mg/kg can reduce the increased activity of LDH in serum (P < 0.05) induced by isoprenaline, but almost had no effect on ST-segment shift in ECG.


CONCLUSIONS
MDG-1 can alleviate IRI isolated myocardium of guinea pigs, and oral administration of MDG-1 showed a definite protection on isoprenaline caused rats' myocardial ischemia damage.
Zheng
Qin
Q
Department of Pharmacy, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai. fyi@vip.sina.com
Feng
Yi
Y
Xu
De-Sheng
DS
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003326:Coronary Circulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use; D004562:Electrocardiography; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D066298:In Vitro Techniques; D007545:Isoproterenol; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D031441:Ophiopogon / Q000737:chemistry*; D008517:Phytotherapy; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D002316:Cardiotonic Agents; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; C517472:polysaccharide MDG-1; D007545:Isoproterenol