Protective effect of R allele of PON1 gene on the coronary artery disease in the presence of specific genetic background.
BACKGROUND
Genetic susceptibility to CAD may be determined by polymorphic variants of genes encoding isoforms involved in the processes important in the pathogenesis of atherosclerosis, including lipids disorders. Participation of single polymorphic variants is relatively small, however its significance may increase in the presence of specific genetic or environmental background.


OBJECTIVE
The aim of the study was an evaluation a possible association between single polymorphic variants of PON1, APOE, ABCA1 and PPARA genes and CAD and looking for specific multigene genotype patterns which differentiate study groups.


METHODS
We studied 358 subjects:178 patients with angiographically confirmed CAD and 180 blood donors without history of CAD. Polymorphisms were genotyped using PCR-RFLP method.


RESULTS
We observed statistically significant differences in the frequencies of R allele and R allele carriers of PON1 gene between CAD and controls. The distribution of genotypes and alleles of other analyzed genes did not differentiate the study groups, however the presence of specific genotypes (APOE& ndash; epsilon3epsilon3, epsilon3epsilon2, ABCA1 - AG, PPARA - GG) increased the protective effect of R allele.


CONCLUSIONS
The present study revealed an independent protective association between carrier-state of PON1 R allele and CAD. This protective effect was especially strong in the presence of specific genotype arrangements of other analyzed genes.
Balcerzyk
Anna
A
Department of Biochemistry and Medical Genetics, Medical University of Silesia, Medykow Str 18, 40-752 Katowice, Poland. abalcerzyk@slam.katowice.pl
Zak
Iwona
I
Krauze
Jolanta
J
D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000293:Adolescent; D000328:Adult; D000483:Alleles; D001057:Apolipoproteins E / Q000235:genetics; D043303:Aryldialkylphosphatase / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D047493:PPAR alpha / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D018528:ATP-Binding Cassette Transporters; D001057:Apolipoproteins E; D047493:PPAR alpha; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human