title: Influence of physical exercise and food restriction on the biomechanical properties of the femur of ageing male rats.
abstract: BACKGROUND
  Voluntary running in wheels as well as food reduction increase the life spans of rats. Disparate parameters such as the collagen biomarker of ageing and the development of kidney pathologies are decreased by voluntary exercise. There are few reports on the influence of physical exercise and food restriction on the skeleton of male rats. Most investigations initiated rather short-term interventions in 4- to 5-week-old animals and thus studied more the influence of growth than the influence of ageing on the skeleton.
  
  
  OBJECTIVE
  To compare the effects of physical exercise and food restriction on the biomechanical properties of bone tissue of ageing male rats with the interventions starting at the age of 5 months with the end point at 23 months. This enables the study of the influence of these interventions on the ageing of the skeleton.
  
  
  METHODS
  Five groups of male Sprague-Dawley rats were used: baseline (BL), voluntarily running in wheels (RW), food restriction to attain pair weight with RW animals (PW), forced running in treadmills (TM), and sedentary controls (SE). The biomechanical properties of femoral neck, diaphysis, and distal metaphysis were measured.
  
  
  RESULTS
  While the body weights and fat-free mass increased from BL to SE group, the occiput-sacrum length did not increase and the length of the femur increased marginally. These lengths were slightly retarded in RW and PW groups compared to the SE group. The strength of the distal femoral metaphysis decreased from BL to SE group. This decrease was counteracted by physical exercise (RW and TM groups) as well as by food restriction (PW group). In contrast, the strength of the femoral mid-diaphysis did not differ between BL and SE groups.
  
  
  CONCLUSIONS
  The distal metaphysis in the male rat femur is more prone to decreasing biomechanical strength than the diaphysis during ageing. Physical exercise, when started at the age of 5 months, when the skeleton has reached its adult size, is somewhat effective in counteracting these changes. There is also some retarding effect of food restriction.
authors.affiliation: Department of Connective Tissue Biology, Institute of Anatomy, University of Aarhus, Arhus, Denmark.
authors.forename: Jesper Skovhus
authors.initials: JS
authors.lastname: Thomsen
authors.forename: Monika
authors.initials: M
authors.lastname: Skalicky
authors.forename: Andrus
authors.initials: A
authors.lastname: Viidik
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D001696:Biomechanical Phenomena; D031204:Caloric Restriction; D003094:Collagen / Q000378:metabolism; D005269:Femur / Q000254:growth & development* / Q000502:physiology; D008297:Male; D010805:Physical Conditioning, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley / Q000502:physiology
chemical_list: D003094:Collagen