title: Daphne decline: what is the causal agent(s)?
abstract: A double infection of Daphne mosaic virus (DapMV) and an associated bacilliform virus was observed in the samples of diseased Daphne mezereum shrubs that showed mosaic patterns, precocious leaves reddening, defoliation, repeated flowering with subsequent declining. Extensive aggregations of bacilliform particles (166-370 x 65 nm and 169-233 x 68-78 nm) occurred in the nucleus or perinuclear space of root and leaf tissues suggesting that the virus might belong to the genus Nucleorhabdovirus, family Rhabdoviridae. However, the single bacilliform virions appeared occasionally in the cytoplasm of the leaf cells. We supposed that occurrence of the mixed virus infection could be the cause of D. mezereum decline in the Czech Republic.
authors.affiliation: Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Plant Molecular Biology, Department of Plant Virology, Ceské Budejovice, Czech Republic. jana@umbr.cas.cz
authors.forename: J
authors.initials: J
authors.lastname: Fránová
authors.forename: D-E
authors.initials: DE
authors.lastname: Lesemann
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D018153:Czech Republic; D032364:Daphne / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000648:ultrastructure / Q000821:virology; D018517:Plant Roots / Q000648:ultrastructure / Q000821:virology; D010942:Plant Viruses / Q000302:isolation & purification* / Q000648:ultrastructure