title: Improved high-performance liquid chromatographic detection of paclitaxel in patient's plasma using solid-phase extraction, and semi-micro-bore C18 separation and UV detection.
abstract: Although a number of analytical methods for taxanes have been published, none of them are sufficiently suitable for use in a medical setting. In this study, we established an improved analytical HPLC/UV detection method using a Sep-Pak C18 cartridge for extraction and a semi-micro-borecolumn for separation. This method employed here reduced chromatographic background signals, and allowed a more sensitive analysis of taxanes in human blood sample. The recovery of taxanes after the solid-phase extraction procedure was over 90%. Chromatographic separation of paclitaxel and docetaxel was achieved within 30 min with no interference peak by a semi-micro-bore column, packed either with C18 (Wakosil 5C18 RS) or pentafluorophenyl (Curosil/Taxol) materials. The method was reproducible with coefficients of variation less than 6%. This analytical procedure was simple and sensitive with lower quantification limit of 3 ng/ml. The improved sensitivity achieved by the popular HPLC/UV apparatus, which is available in hospitals, would vouch safer and more efficient therapy with taxane.
authors.affiliation: Department of Hospital Pharmacy & Pharmacology, Asahikawa Medical College, Asahikawa 078-8510, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Suno
authors.forename: Takashi
authors.initials: T
authors.lastname: Ono
authors.forename: Shinya
authors.initials: S
authors.lastname: Iida
authors.forename: Noriko
authors.initials: N
authors.lastname: Umetsu
authors.forename: Ko-ichi
authors.initials: K
authors.lastname: Ohtaki
authors.forename: Takehiro
authors.initials: T
authors.lastname: Yamada
authors.forename: Toshio
authors.initials: T
authors.lastname: Awaya
authors.forename: Machiko
authors.initials: M
authors.lastname: Satomi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tasaki
authors.forename: Keiko
authors.initials: K
authors.lastname: Shimizu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Matsubara
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D005260:Female; D006801:Humans; D017239:Paclitaxel / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D014466:Ultraviolet Rays
chemical_list: D017239:Paclitaxel