Evaluation of selected parameters of the antioxidative system in patients with type 2 diabetes in different periods of metabolic compensation.
BACKGROUND
The aim of the study was to evaluate some selected parameters of the antioxidative system in patients with type 2 diabetes.


METHODS
Eighty-one type 2 diabetes patients took part in the study (39 patients with metabolically balanced and 42 with metabolically unbalanced diabetes). The control group consisted of 30 healthy people. The total antioxidant capacity of plasma was measured fluorometrically using phycoerythrin. To calculate the low-molecular-weight antioxidant concentration in the plasma samples, the duration of Trolox activity as a function of its concentration in the sample was measured. The activity of antioxidative enzymes in red blood cells was determined using the Misra and Fridovich method and Beers and Sizer method.


RESULTS
The total plasma antioxidant capacity and the low-molecular-weight antioxidant concentration in the group of patients with metabolically compensated type 2 diabetes were statistically significantly higher than in the group of patients with metabolically uncontrolled diabetes. The activity of antioxidative enzymes was found to be higher in the group of type 2 diabetes patients at the stage of metabolic balance.


CONCLUSIONS
The obtained results confirm the thesis of glucose toxicity and intensification of oxidative stress in patients with diabetes.
Rysz
Jacek
J
2nd Department of Family Medicine, University Hospital No. 2, Medical University of Łódź, Poland. jacek.rysz@skwam.lodz.pl
Błaszczak
Robert
R
Banach
Maciej
M
Kedziora-Kornatowska
Kornelia
K
Kornatowski
Tomasz
T
Tański
Wojciech
W
Kedziora
Józef
J
D000975:Antioxidants / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology
D000975:Antioxidants; D006442:Glycated Hemoglobin