Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay.
Two novel marine, Gram-negative, non-motile, catalase- and oxidase-positive, aerobic bacteria were isolated from coastal seawater in Tokyo Bay. Analysis of almost-complete 16S rRNA gene sequences showed that the two isolates are members of the genus Paracoccus, sharing highest 16S rRNA gene sequence similarity (96.5 %) with Paracoccus aminophilus NBRC 16710(T). The DNA-DNA reassociation values between P. aminophilus NBRC 16710(T) and these isolates were only 10-20 %, in contrast to the high DNA relatedness between the two isolates (89 %). At least 1 % (w/v) NaCl was required for growth. Cellular fatty acid profiles revealed C(18 : 1)omega7c as the major component and C(10 : 0) 3-OH as the major hydroxy fatty acid. Ubiquinone-10 was detected as the major respiratory quinone. The G+C content of the genomic DNA of both strains was 69 mol%. On the basis of DNA-DNA hybridization data and physiological and chemotaxonomic characteristics, it is proposed that these strains should be placed in a novel species, Paracoccus marinus sp. nov. The type strain is KKL-A5(T) (=NBRC 100637(T) =CIP 108500(T)); KKL-B9 (=NBRC 100640) is a reference strain.
Khan
Shams Tabrez
ST
Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8, Kazusakamatari, Kisarazu-shi, Chiba 292-0818, Japan. shams-tabrez-khan@nite.go.jp
Takaichi
Shinichi
S
Harayama
Shigeaki
S
D015373:Bacterial Typing Techniques; D002338:Carotenoids / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D005960:Glucosides / Q000096:biosynthesis*; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010230:Paracoccus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D005960:Glucosides; D012336:RNA, Ribosomal, 16S; C516381:adonixanthin diglucoside; D002338:Carotenoids