title: Transcript-specific translational regulation in the unfolded protein response of Saccharomyces cerevisiae.
abstract: Accumulation of unfolded proteins in the endoplasmic reticulum (ER) causes stress and induces the unfolded protein response (UPR). Genome-wide analysis of translational regulation in response to the UPR-inducing agent dithiothreitol in Saccharomyces cerevisiae is reported. Microarray analysis, confirmed using qRT-PCR, identified transcript-specific translational regulation. Transcripts with functions in ribosomal biogenesis and assembly were translationally repressed. In contrast, mRNAs from known UPR genes, encoding the UPR transcription factor Hac1p, the ER-oxidoreductase Ero1p and the ER-associated protein degradation (ERAD) protein Der1p, were enriched in polysomal fractions, indicating translational up-regulation. Splicing of HAC1 mRNA is shown to be required for efficient ribosomal loading.
authors.affiliation: School of Biology, University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: Tom
authors.initials: T
authors.lastname: Payne
authors.forename: Colin
authors.initials: C
authors.lastname: Hanfrey
authors.forename: Amy L
authors.initials: AL
authors.lastname: Bishop
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Michael
authors.forename: Simon V
authors.initials: SV
authors.lastname: Avery
authors.forename: David B
authors.initials: DB
authors.lastname: Archer
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins