Why 3-D? Gel-based microarrays in proteomics.
Gel-based microarrays (biochips) consisting of nanoliter and sub-nanoliter gel drops on hydrophobic substrate are a versatile technology platform for immobilization of proteins and other biopolymers. Biochips provide a highly hydrophilic environment, which stabilizes immobilized molecules and facilitates their interactions with analytes. The probes are immobilized simultaneously with gel polymerization, evenly distributed throughout individual elements, and are easily accessible because of large pores. Each element is an isolated nanotube. Applications of biochips in the studies of protein interactions with other proteins, nucleic acids, and glycans are described. In particular, biochips are compatible with MALDI-MS. Biochip-based assay of prostate-specific antigen became the first protein microarray approved for clinical use by a national regulatory agency. In this review, 3-D immobilization is compared with mainstream technologies based on surface immobilization.
Rubina
Alla Yu
AY
Engelhardt Institute of Molecular Biology of Russian Academy of Sciences, Moscow, Russia.
Kolchinsky
Alexander
A
Makarov
Alexander A
AA
Zasedatelev
Alexander S
AS
D014408:Biomarkers, Tumor / Q000032:analysis; D004058:Diffusion; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D011134:Polysaccharides / Q000032:analysis; D017430:Prostate-Specific Antigen / Q000032:analysis; D040081:Protein Array Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014118:Toxins, Biological / Q000032:analysis
D014408:Biomarkers, Tumor; D020100:Hydrogels; D011134:Polysaccharides; D014118:Toxins, Biological; D017430:Prostate-Specific Antigen