Potato glycoalkaloids in soil-optimising liquid chromatography-time-of-flight mass spectrometry for quantitative studies.
Potato glycoalkaloids are produced in high amounts in potato fields during the growth season and losses to soil potentially impact shallow groundwater and via tiles to fresh water ecosystems. A quantitative liquid chromatography-electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS) method for determination and quantification of potato glycoalkaloids and their metabolites in aqueous soil extracts was developed. The LC-ESI-TOF-MS method had linearities up to 2000microg/L for alpha-solanine and alpha-chaconine and up to 760microg/L for solanidine. No matrix effect was observed, and the detection limits found were in the range 2.2-4.7microg/L. The method enabled quantification of the potato glycoalkaloids in environmental samples.
Jensen
Pia H
PH
Department of Natural Sciences, Faculty of Life Sciences (LIFE), University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg, Denmark. phj@life.ku.dk
Juhler
René K
RK
Nielsen
Nikoline J
NJ
Hansen
Thomas H
TH
Strobel
Bjarne W
BW
Jacobsen
Ole S
OS
Nielsen
John
J
Hansen
Hans Christian B
HC
D002853:Chromatography, Liquid / Q000379:methods*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012987:Soil / Q000032:analysis*; D012991:Solanaceous Alkaloids / Q000032:analysis* / Q000737:chemistry; D012992:Solanine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D012987:Soil; D012991:Solanaceous Alkaloids; C514919:alpha-solanine; D012992:Solanine; C011860:alpha-chaconine