title: [Hyperplasia suppressor gene expression in vascular smooth muscle cells derived from normotensive and hypertensive patients underwent bypass surgery].
abstract: OBJECTIVE
  To investigate the novel hyperplasia suppressor gene (HSG) expression in vascular smooth muscle cells derived from normotensive and hypertensive patients underwent bypass surgery.
  
  
  METHODS
  Coronary heart disease patients underwent coronary artery bypass graft (CABG) operation in BEIJING ANZHEN hospital from 4 - 9, 2006 were enrolled in this study and divided into hypertensive group (n = 28) and normotensive group (n = 26). The preoperative venous blood samples were taken for serum biochemical and vasoactive peptides measurements. Total RNA was extracted from WBC, explanted-vessels and cultured VSMCs using TRIZOL and HSG expression was determined by Semi-Quantitative RT-PCR.
  
  
  RESULTS
  Body mass index (BMI) was significantly higher in hypertensive group compared to normotensive group (P < 0.01) while other biochemic parameters and vasoactive peptides were similar between the groups. BMI and GLU, BMI and SBP, BMI and DBP, GLU and TG, SBP and DBP were positively correlated (all P < 0.05). HSG expression in WBC, VSMCs and vessel tissue were significantly lower in hypertensive group than those in normotensive group (all P < 0.05). HSG expression in tissue was negatively correlated to BMI, SBP and DBP (all P < 0.05).
  
  
  CONCLUSIONS
  Reduced HSG expression and the negative correlation on vascular tissue HSG expression to BMI, SBP and DBP suggested a possible inhibitory role of HSG on VSMC proliferation and blood pressure.
authors.affiliation: Hypertension Research Lab, Beijing Institute of Heart, Lung, Vascular Disease, Beijing Anzhen Hospital, Capital University of Medical Science, Beijing 100029, China.
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Liu
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Wen
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Li-min
authors.initials: LM
authors.lastname: Zhao
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Guo
authors.forename: Xin-jun
authors.initials: XJ
authors.lastname: Chen
authors.forename: Zuo-guang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Jie-lin
authors.initials: JL
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Wen
authors.forename: Shi-Qi
authors.initials: SQ
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
mesh_terms: D000368:Aged; D015992:Body Mass Index; D005260:Female; D015870:Gene Expression; D016153:Genes, Suppressor; D006801:Humans; D006965:Hyperplasia / Q000235:genetics; D006973:Hypertension / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism*