title: Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor.
abstract: We report here the generation of transgenic chickens that produce human granulocyte-colony stimulating factor (hG-CSF) using replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G). The recombinant retrovirus was injected beneath the blastoderm of nonincubated chicken embryos (stage X). Out of 140 injected eggs, 17 chicks hatched after 21 days of incubation and all hatched chicks were found to express vector-encoded hG-GSF gene. The biological activity of the recombinant hG-CSF was significantly higher than its commercially derived E. coli-derived counterpart. Successful germline transmission of the transgene was also confirmed in G(1) transgenic chicks produced from the cross of Go transgenic roosters with nontransgenic hens, but most of the G(1) progeny were dead within 1 month of hatching.
authors.affiliation: Department of Physiology, Catholic University of Daegu School of Medicine, Daegu, Korea.
authors.forename: Mo Sun
authors.initials: MS
authors.lastname: Kwon
authors.forename: Bon Chul
authors.initials: BC
authors.lastname: Koo
authors.forename: Bok Ryul
authors.initials: BR
authors.lastname: Choi
authors.forename: Yoon-Yub
authors.initials: YY
authors.lastname: Park
authors.forename: Young Man
authors.initials: YM
authors.lastname: Lee
authors.forename: Hun Suk
authors.initials: HS
authors.lastname: Suh
authors.forename: Young Sik
authors.initials: YS
authors.lastname: Park
authors.forename: Hoon Taek
authors.initials: HT
authors.lastname: Lee
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.forename: Ji Yeol
authors.initials: JY
authors.lastname: Roh
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Teoan
authors.initials: T
authors.lastname: Kim
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques; D002642:Chick Embryo / Q000502:physiology; D002645:Chickens; D005260:Female; D005307:Fertilization in Vitro; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D010063:Ovum / Q000166:cytology / Q000502:physiology*; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics; D012661:Semen
chemical_list: D016179:Granulocyte Colony-Stimulating Factor