SOCS1 downregulation in dendritic cells promotes memory T-cell responses.
SOCS1-1 is crucial for control of immune cell cytokine expression, including those cytokines known to enable memory T-cell formation and homeostasis. In this study, we found that immunization with SOCS1-downregulated bone marrow-derived dendritic cells generated increased antigen-specific CD8(+) T memory cells and antigen-specific responses, as measured by ELISPOT, CTL assays, serum ELISAs, and T-cell proliferation assays. Bone marrow-derived dendritic cells in which SOCS1 was downregulated expressed increased levels of surface IL-15Ra and thymic leukemia (TL) antigen, both of which support memory cell development. This work supports a crucial role for SOCS1 in regulation of dendritic cell-directed generation of memory T-cell responses.
Aldrich
Melissa
M
Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA.
Sanders
Denise
D
Lapteva
Natasha
N
Huang
Xue F
XF
Chen
Si-Yi
SY
D000818:Animals; D000906:Antibodies / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology*; D015536:Down-Regulation; D005260:Female; D005434:Flow Cytometry; D007156:Immunologic Memory; D053720:Interleukin-15 Receptor alpha Subunit / Q000096:biosynthesis; D016131:Lymphocyte Subsets / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010047:Ovalbumin / Q000276:immunology; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis* / Q000276:immunology*
D000906:Antibodies; D053720:Interleukin-15 Receptor alpha Subunit; D008562:Membrane Glycoproteins; C491590:Socs1 protein, mouse; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins; C028388:thymus-leukemia antigens; D010047:Ovalbumin