Studies of benzothiazole and benzoselenazole squaraines as fluorescent probes for albumins detection.
Series of squaraine benzothiazole and benzoselenazole dyes were studied as possible fluorescent probes for the detection of proteins, particularly albumins. It was shown that majority of the studied squaraines give significant fluorescent response on the human serum albumin (HSA) and bovine serum albumin presence. For squaraine dyes with N-hexyl pendent groups (P-1, P-2, P-3, P-5) about 100-540-fold fluorescence intensity increase upon albumins addition was observed. At the same time in presence of other proteins, namely insulin, avidin from hen egg white, immunoglobulin G (IgG), carbonic anhydrase fluorescence enhancement values were considerably lower -up to 43 times in IgG presence. It was noted that generally, squaraines with long N-hexyl pendent groups demonstrate higher emission increase values upon proteins addition comparing with their analogues with short N-ethyl tails. It was shown that fluorescence intensity enhancement for benzothiazole squaraine dye P-3, relates linearly to the HSA concentration over the wide range-from 0.2 to 500 microg/ml. Together with noticeable selectivity of this dye to albumins, existence of wide dynamic range gives possibility to propose P-3 dye as probe for HSA quantification.
Volkova
Kateryna D
KD
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo St., 03143, Kyiv, Ukraine.
Kovalska
Vladyslava B
VB
Losytskyy
Mykhaylo Yu
MY
Bento
Artur
A
Reis
Lucinda V
LV
Santos
Paulo F
PF
Almeida
Paulo
P
Yarmoluk
Sergiy M
SM
D000418:Albumins / Q000032:analysis*; D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry*; D002021:Buffers; D002417:Cattle; D003503:Cyclobutanes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
D000418:Albumins; D052160:Benzothiazoles; D002021:Buffers; D003503:Cyclobutanes; D005456:Fluorescent Dyes; D010636:Phenols; D012709:Serum Albumin; C480596:squaraine; D014867:Water; D012710:Serum Albumin, Bovine; C005465:benzothiazole