title: Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi.
abstract: Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3beta-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [(3)H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition.
authors.affiliation: Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL 61802, United States.
authors.forename: María Laura
authors.initials: ML
authors.lastname: Salto
authors.forename: Theresa
authors.initials: T
authors.lastname: Kuhlenschmidt
authors.forename: Mark
authors.initials: M
authors.lastname: Kuhlenschmidt
authors.forename: Rosa M
authors.initials: RM
authors.lastname: de Lederkremer
authors.forename: Roberto
authors.initials: R
authors.lastname: Docampo
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D006017:Glycolipids / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015388:Organelles / Q000737:chemistry* / Q000648:ultrastructure; D010743:Phospholipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000648:ultrastructure*
chemical_list: D006017:Glycolipids; D010743:Phospholipids; D002118:Calcium