[Effects of endotheline and nitric oxide on vascular tonicity in patients with chronic cardiac failure].
OBJECTIVE
To examine effects of endotheline-1 (ET-1) and nitric oxide (NO) on endothelium-dependent mechanisms of vascular tonicity regulation in patients with chronic cardiac failure (CCF) of FC I-IV (NYHA).


METHODS
Vascular reactions of 94 patients with CCF of FC I-IV were examined according to D.S. Celermajer method using ultrasound of high resolution. Tissue oxygenation was studied with transcutaneous polarography (TCM-2, Radiometer). Parameters of circulating erythrocytes were studied by impurity spectrum. Endothelial NO-synthase expression was determined with application of monoclonal antibodies to endothelial NO-synthase. NO metabolites were studied with colorimetric method using Griss reagent. ET-1 was measured in plasma by enzyme immunoassay. The control group consisted of 28 healthy men aged 20 to 54 years.


RESULTS
Patients with CCF of FC I-IV have reduced endothelium-dependent vascular reactions progressing with aggravation of CCF. The causes of the disorders lie in altered metabolism of ET-1 and NO in CCF.


CONCLUSIONS
CCF patients demonstrate changes in endothelium-dependent mechanisms of vascular tonicity regulation caused by disturbed metabolism of ET-1 and NO developing in abnormal regime of tissue oxygenation and depending on CCF severity.
Denisov
E N
EN
Kots
Ia I
IaI
Bakhtiiarov
R Z
RZ
Gumanova
N G
NG
D000328:Adult; D001916:Brachial Artery / Q000000981:diagnostic imaging / Q000503:physiopathology*; D003124:Colorimetry; D019332:Endothelin-1 / Q000097:blood*; D004730:Endothelium, Vascular / Q000201:enzymology; D006333:Heart Failure / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis; D011048:Polarography; D011379:Prognosis; D012720:Severity of Illness Index; D014463:Ultrasonography; D014655:Vascular Resistance / Q000502:physiology*
D019332:Endothelin-1; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase