title: Effects of hemostatic agents on the histomorphologic response of human dental pulp capped with calcium hydroxide.
abstract: OBJECTIVE
  To evaluate the response of human pulps capped with a calcium hydroxide [Ca(OH)2] cement after bleeding control with 2 hemostatic agents.
  
  
  METHODS
  Pulps were exposed on the occlusal floor, and the bleeding was controlled either with saline solution (SS) or 2.5% sodium hypochlorite (NaOCl) (SH). After that, the pulp was capped with Ca(OH)2 cement and restored with resin composite. After 30 (groups SS30 and SH30) and 60 (groups SS60 and SH60) days, the teeth were extracted and processed with hematoxylin-eosin and categorized in a histologic score system. The data were subjected to Kruskal-Wallis and Mann-Whitney tests (alpha = .05).
  
  
  RESULTS
  Regarding dentin bridge formation, an inferior response of SH60 group was observed when compared to SS60 (P < .05). The response of the SH30 group generally was similar to that of the groups treated with saline solution. However, after 60 days, 2.5% NaOCl showed a trend toward having an inferior response.
  
  
  CONCLUSIONS
  Using saline solution as a hemostatic agent before pulp capping with Ca(OH)2 resulted in a significantly better histomorphologic response than using 2.5% NaOCl as a hemostatic agent before capping with Ca(OH)2.
authors.affiliation: Department of Dental Materials, School of Dentistry, University of São Paulo, São Paulo, Brazil.
authors.forename: Maria de Lourdes Rodrigues
authors.initials: Mde L
authors.lastname: Accorinte
authors.forename: Alessandro D
authors.initials: AD
authors.lastname: Loguercio
authors.forename: Alessandra
authors.initials: A
authors.lastname: Reis
authors.forename: Robert
authors.initials: R
authors.lastname: Holland
mesh_terms: D000293:Adolescent; D000328:Adult; D001641:Bicuspid; D002126:Calcium Hydroxide; D003738:Dental Cements; D003782:Dental Pulp / Q000098:blood supply / Q000187:drug effects*; D003785:Dental Pulp Capping; D003790:Dental Pulp Necrosis / Q000139:chemically induced; D003809:Dentin, Secondary / Q000378:metabolism / Q000473:pathology; D006490:Hemostatics / Q000009:adverse effects*; D006801:Humans; D006472:Oral Hemorrhage / Q000517:prevention & control*; D011671:Pulpitis / Q000139:chemically induced; D012965:Sodium Chloride / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000009:adverse effects*
chemical_list: D003738:Dental Cements; D006490:Hemostatics; D012965:Sodium Chloride; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide