title: Molecular mechanism of force generation by dynein, a molecular motor belonging to the AAA+ family.
abstract: Dynein is an AAA+ (ATPase associated with various cellular activities)-type motor complex that utilizes ATP hydrolysis to actively drive microtubule sliding. The dynein heavy chain (molecular mass >500 kDa) contains six tandemly linked AAA+ modules and exhibits full motor activities. Detailed molecular dissection of this motor with unique architecture was hampered by the lack of an expression system for the recombinant heavy chain, as a result of its large size. However, the recent success of recombinant protein expression with full motor activities has provided a method for advances in structure-function studies in order to elucidate the molecular mechanism of force generation.
authors.affiliation: Department of Life Sciences, University of Tokyo, Komaba 3-8-1, Meguro, Tokyo 153-8902, Japan. numata@bio.c.u-tokyo.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Numata
authors.forename: Takahide
authors.initials: T
authors.lastname: Kon
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shima
authors.forename: Kenji
authors.initials: K
authors.lastname: Imamula
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Mogami
authors.forename: Reiko
authors.initials: R
authors.lastname: Ohkura
authors.forename: Keiko
authors.initials: K
authors.lastname: Sutoh
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sutoh
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D004398:Dyneins / Q000737:chemistry / Q000378:metabolism*; D008666:Metalloendopeptidases / Q000737:chemistry / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D017434:Protein Structure, Tertiary
chemical_list: D000255:Adenosine Triphosphate; D008666:Metalloendopeptidases; C114583:m-AAA proteases; D004398:Dyneins