Poly I:C induces Mx transcription and promotes an antiviral state against sole aquabirnavirus in the flatfish Senegalese sole (Solea senegalensis Kaup).
Mx is an interferon-induced protein that protects against viral infections. In this study the absolute number of Mx transcripts after poly I:C injection (a synthetic dsRNA) or sole aquabirnavirus (solevirus) inoculation in Senegalese sole (Solea senegalensis Kaup) has been quantified. Mx expression profiles differed clearly in both experimental conditions; the induction response was faster and more intense after poly I:C injection than after solevirus inoculation. Moreover, pre-injection of soles with poly I:C prior to solevirus infection eliminated the induction of Mx expression associated with this virus. To evaluate the possible interference of poly I:C treatments on solevirus replication, the mRNA levels of the virus capsid protein (VP2) were determined by RT-PCR. VP2 transcripts were hardly detected in poly I:C pre-injected animals from 12 to 72 h after solevirus inoculation. All these data suggest that poly I:C is able to induce an antiviral state that interferes with solevirus replication, and support the suitability of Mx expression analysis as a marker to study the defensive response against solevirus.
Fernandez-Trujillo
A
A
Department of Genetics, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain.
Ferro
P
P
Garcia-Rosado
E
E
Infante
C
C
Alonso
M C
MC
Bejar
J
J
Borrego
J J
JJ
Manchado
M
M
D000818:Animals; D000998:Antiviral Agents / Q000276:immunology; D019201:Aquabirnavirus / Q000276:immunology*; D018175:Birnaviridae Infections / Q000235:genetics / Q000662:veterinary / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000821:virology*; D005412:Flatfishes / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D019204:GTP-Binding Proteins / Q000235:genetics*; D064547:Myxovirus Resistance Proteins; D011070:Poly I-C / Q000276:immunology*; D014158:Transcription, Genetic; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D036022:Capsid Proteins; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins; D011070:Poly I-C