Validation of a Biacore method for screening eight sulfonamides in milk and porcine muscle tissues according to European decision 2002/657/EC.
Sulfonamides are commonly used for prophylactic or therapeutic purposes in veterinary medicine. A maximum residue limit (MRL) for sulfonamides has been set at 100 microg/kg in milk and muscle. A multisulfonamide antibody was used for the development of 2 different Biacore protocols, one for the screening of milk samples, the other for muscle samples. Two different Biacore systems were used: Biacore X system (milk protocol), which is considered a research and development apparatus, and Biacore 3000 system (muscle protocol), which is a completely automated system used for high-throughput screening. This report describes the validation of semiquantitative immunological methods according to the European Decision 2002/657/EC "concerning the performance of analytical methods." The different performance characteristics (detection capability CCbeta, specificity/selectivity, precision, stability, and applicability) were determined in relation to the European Union MRL of 100 microg/kg for sulfonamides. The applicability of the method to porcine, bovine, and poultry muscle was studied. The detection capabilities CCbeta were calculated to be 40 microg/L in milk and 60 microg/kg in porcine, bovine, and poultry muscles. Eight different sulfonamides, of which 3 (sulfamethazine, sulfamerazine, and sulfadiazine) are authorized in France, were detected simultaneously, at or below the MRL level, with both Biacore systems.
Gaudin
Valérie
V
CRL-AFSSA, BP 90203, 35302 Fougères, France. v.gaudin@fougeres.afssa.fr
Hédou
Céline
C
Sanders
Pascal
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D015374:Biosensing Techniques; D004350:Drug Residues; D005062:European Union; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
D000900:Anti-Bacterial Agents; D012996:Solutions; D013449:Sulfonamides