DP reactive antibody in a zero mismatch renal transplant pair.
This study describes molecular basis for positive B-cell flow crossmatch in a zero mismatch (0MM) transplant pair. Our end-stage renal disease patient was B-cell flow crossmatch positive with a 0MM deceased donor. DNA from the donor and patient were further typed by a high-resolution method. The patient's sera were tested for anti-HLA reactivity by single antigen bead using a Luminex platform. The patient and donor were found to be HLA identical except for a single DP allele MM (DPB1*0601). As there was no single antigen bead coated with DPB*0601, analysis of the amino acid residues of reactive and nonreactive DPB1 alleles was conducted. The results showed that all reactive alleles carried the amino acid D-E at residue 55 and 56 of DPB1. The MM allele DPB1*0601 also carries the DE 55-56 epitope. We conclude that positive B-cell flow crossmatch was likely the result of single MM in the DP locus.
Vaidya
Smita
S
Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA. svaidya@utmb.edu
Hilson
Brant
B
Sheldon
Susan
S
Cano
Pedro
P
Fernandez-Vina
Marcelo
M
D000328:Adult; D000483:Alleles; D000906:Antibodies; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006682:HLA-DP Antigens / Q000235:genetics / Q000276:immunology*; D059868:HLA-DP beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000097:blood* / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D014019:Tissue Donors
D000906:Antibodies; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D007126:Immunoglobulin Allotypes