Cyclic regulation of T-Bet and GATA-3 in human endometrium.
Endometrial cytokine expression is poorly understood. T-Bet and GATA-3 regulate cytokine expression in T-lymphocytes. Previous work has demonstrated expression of T-Bet in human endometrium. Changes in human endometrial T-Bet and GATA-3 mRNA and protein expression during the normal menstrual cycle were characterized. Human endometrium from each phase of the menstrual cycle underwent real-time reverse-transcriptase polymerase chain reaction and immunohistochemistry to examine expression and localization. T-Bet and GATA-3 mRNA were increased in the late secretory phase. Progesterone receptor (PR) mRNA was increased during the proliferative and early secretory phases. T-Bet and GATA-3 proteins localized cytoplasmically in the late secretory phase. PR protein displayed nuclear localization and maximal immunostaining during the early secretory phase. T-Bet and GATA-3 are expressed in endometrial epithelium cyclically during the menstrual cycle. T-Bet and GATA-3 are both upregulated during the late secretory phase and in the same cell types. The expression patterns of T-Bet and GATA-3 oppose PR, suggesting antagonistic function and/or regulation between PR and T-Bet/GATA-3.
Inman
Danielle
D
Department of Obstetrics & Gynecology, University of North Carolina at Chapel Hill, NC 27599, USA. danielle_inman@med.unc.edu
Kawana
Kei
K
Schust
Danny
D
Lininger
Ruth
R
Young
Steven
S
D000328:Adult; D004717:Endometrium / Q000502:physiology*; D005260:Female; D050990:GATA3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D000098815:T-bet Transcription Factor
D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D007986:Luteinizing Hormone