Oxidized low-density lipoprotein induces differentiation of RAW264.7 murine macrophage cell line into dendritic-like cells.
Dendtritic cells (DCs) are potent antigen-presenting cells and have an important role in the pathogenesis of atherosclerosis. Recent data suggests oxidized low-density lipoprotein (oxLDL) promotes the transition of a differentiating monocyte to a mature dendritic cell. In this study, we examined whether oxLDL could induce the differentiation of mature macrophages into DCs. After 48 h treatment with oxLDL, RAW264.7 cells increased in cell size and exhibited dendritic morphology. At the optimal oxLDL dose (10 microg/ml), approximately 74% of RAW264.7 cells differentiated into dendritic-like cells. Flow cytometric analysis detected dendritic cell surface markers (CD83, CD40, CD86, MHC Class II, and CD1d), and their expression increased in a dose- and time-dependent manner. Moreover, oxLDL-treated RAW264.7 cells showed functional changes including reduced endocytic activity, increased allostimulatory activity, and IL-12 production. The findings of the present work demonstrate that RAW264.7 cells, incubated with oxLDL, acquire some dendritic cell features.
Shen
Ling-Hong
LH
Department of Cardiology, Ren Ji Hospital, Medical School of Shanghai Jiao Tong University, Shanghai 200127, China.
Zhou
Lei
L
Wang
Bin-Yao
BY
Pu
Jun
J
Hu
Liu-Hua
LH
Chai
Da-Jun
DJ
Wang
Li
L
Zeng
Jin-Zhang
JZ
He
Ben
B
D000818:Animals; D050197:Atherosclerosis; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D016207:Cytokines; D008077:Lipoproteins, LDL; D010100:Oxygen