title: Cell response to magnetic glyconanoparticles: does the carbohydrate matter?
abstract: Magnetic nanoparticles have been used in therapeutic and diagnostic approaches in biomedicine for many years. For these applications, it is very important to investigate the nanoparticle-cell interactions. In this study we report a simple method for the preparation of gold-iron nanoparticles protected and functionalized with biologically relevant saccharides (maltose, lactose, and glucose). The nanoparticles were subsequently tested in vitro with a human fibroblast cell line to determine biocompatibility, and the cell-particle interactions, using fluorescence and scanning electron microscopies. Different cellular responses were obtained for each type of glyconanoparticle, demonstrating that the cells can recognize the saccharides on the nanoparticles.
authors.affiliation: Laboratory of Glyconanotechnology, IIQ-CSIC, 41092 Seville, Spain. jmfuente@unizar.es
authors.forename: Jesús M
authors.initials: JM
authors.lastname: de la Fuente
authors.forename: David
authors.initials: D
authors.lastname: Alcántara
authors.forename: Soledad
authors.initials: S
authors.lastname: Penadés
mesh_terms: D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000633:toxicity*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003603:Cytotoxins / Q000378:metabolism; D004705:Endocytosis; D005290:Ferric Compounds / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006801:Humans; D008280:Magnetics; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000032:analysis / Q000633:toxicity*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D002241:Carbohydrates; D003603:Cytotoxins; D005290:Ferric Compounds; D006046:Gold