Morphogenesis of post-Golgi transport carriers.
The trans-Golgi network (TGN) is one of the main, if not the main, sorting stations in the process of intracellular protein trafficking. It is therefore of central importance to understand how the key players in the TGN-based sorting and delivery process, the post-Golgi carriers (PGCs), form and function. Over the last few years, modern morphological approaches have generated new insights into the questions of PGC biogenesis, structure and dynamics. Here, we present a view by which the "lifecycle" of a PGC consists of several distinct stages: the formation of TGN tubular export domains (where different cargoes are segregated from each other and from the Golgi enzymes); the docking of these tubular domains onto molecular motors and their extrusion towards the cell periphery along microtubules; the fission of the forming PGC from the donor membrane; and the delivery of the newly formed PGC to its specific acceptor organelle. It is now important to add the many molecular machineries that have been described as operating at the TGN to this "morphofunctional map" of the TGN export process.
Luini
Alberto
A
Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030, Santa Maria Imbaro (Chieti), Italy. luini@negrisud.it
Mironov
Alexander A
AA
Polishchuk
Elena V
EV
Polishchuk
Roman S
RS
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D006801:Humans; D022161:Transport Vesicles / Q000378:metabolism*; D021601:trans-Golgi Network / Q000502:physiology* / Q000648:ultrastructure