Effects of aspirin on the development of Helicobacter pylori-induced gastric inflammation and heterotopic proliferative glands in Mongolian gerbils.
BACKGROUND
Helicobacter pylori infection is a major cause of gastritis and gastric carcinoma. Aspirin has anti-inflammatory and antineoplastic activity. The aim of the present study was to determine the effects of aspirin on H. pylori-induced gastritis and the development of heterotopic proliferative glands.


METHODS
H. pylori strain SS1 was inoculated into the stomachs of Mongolian gerbils. Two weeks after inoculation, the animals were fed with the powder diets containing 0 p.p.m. (n = 10), 150 p.p.m. (n = 10), or 500 p.p.m. (n = 10) aspirin. Mongolian gerbils were killed after 36 weeks of infection. Uninfected Mongolian gerbils (n = 10) were used as controls. Histologic changes, epithelial cell proliferation and apoptosis, and prostaglandin E(2) (PGE(2)) levels of gastric tissue were determined.


RESULTS
H. pylori infection induced gastric inflammation. Administration of aspirin did not change H. pylori-induced gastritis, but alleviated H. pylori-induced hyperplasia and the development of heterotopic proliferative glands. Administration of aspirin accelerated H. pylori-associated apoptosis but decreased H. pylori-associated cell proliferation. In addition, the increased gastric PGE(2) levels due to H. pylori infection were suppressed by treatment with aspirin, especially at the dose of 500 p.p.m.


CONCLUSIONS
Aspirin alleviates H. pylori-induced hyperplasia and the development of heterotopic proliferative glands. Moreover, aspirin increases H. pylori-induced apoptosis. We demonstrated the antineoplastic activities of aspirin in H. pylori-related gastric carcinogenesis.
Li
Guo Qing
GQ
Department of Medicine, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Xia
Harry H X
HH
Chen
Min Hu
MH
Tsukamoto
Tetsuya
T
Tatematsu
Masae
M
Gu
Qing
Q
Qiao
Liang
L
Cho
C H
CH
So
Wallace H L
WH
Yuen
M F
MF
Hu
Pin Jin
PJ
Liang
Ying Jie
YJ
Lin
Han Liang
HL
Chan
Annie O O
AO
Wong
Benjamin C Y
BC
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology*; D002828:Choristoma / Q000473:pathology / Q000517:prevention & control*; D015232:Dinoprostone / Q000032:analysis; D004847:Epithelial Cells / Q000473:pathology; D005753:Gastric Mucosa / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000502:physiology*; D006965:Hyperplasia / Q000517:prevention & control; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control*; D008297:Male
D000893:Anti-Inflammatory Agents; D015232:Dinoprostone; D001241:Aspirin