Effect of hypoxia on stromal precursors from rat bone marrow at the early stage of culturing.
We studied the effect of hypoxia on stromal precursors from rat bone marrow. Under hypoxic conditions the cultures were characterized by low heterogeneity, the percent of large spread morphologically different cells decreased. Hypoxia increased proliferative activity of cells, had no adverse effect, and decreased the percent of cells expressing hemopoietic markers.
Buravkova
L B
LB
State Research Center of the Russian Federation, Institute of Medical and Biological Problems, Russian Academy of Medical Sciences, Moscow. buravkova@imbp.ru
Anokhina
E B
EB
D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005434:Flow Cytometry; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D051381:Rats; D017154:Stromal Cells / Q000166:cytology* / Q000276:immunology