title: Prevalence and abundance of uncultivated Megasphaera-like bacteria in the human vaginal environment.
abstract: Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests that both phylotypes are present in higher concentrations in women with bacterial vaginosis.
authors.affiliation: The Research Institute for Children,1 New Orleans, Louisiana, USA.
authors.forename: Marcela
authors.initials: M
authors.lastname: Zozaya-Hinchliffe
authors.forename: David H
authors.initials: DH
authors.lastname: Martin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Ferris
mesh_terms: D001483:Base Sequence; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D008145:Louisiana / Q000453:epidemiology; D045851:Megasphaera / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S