title: Beta-synuclein protein from Xenopus laevis: overexpression in Escherichia coli of the GST-tagged protein and production of polyclonal antibodies.
abstract: This report presents a procedure to obtain and purify recombinant beta-synuclein from Xenopus laevis expressed in Escherichia coli as a glutathione-S-transferase fusion protein. After identification by mass spectrometry, the protein was then used to raise anti-X. laevis beta-synuclein polyclonal antibodies, which were suitable to detect the presence of beta-synuclein in X. laevis brain by Western blot. This is the first report of a positive identification of beta-synuclein in an amphibian at the protein level.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School and College of Life Science, Sichuan University, Chengdu, Sichuan, 610041, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Yuan
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.forename: Huanyi
authors.initials: H
authors.lastname: Liu
authors.forename: Shaoquan
authors.initials: S
authors.lastname: Xiong
authors.forename: Jiong
authors.initials: J
authors.lastname: Li
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001921:Brain / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D021241:Spectrometry, Mass, Electrospray Ionization; D029867:Xenopus Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D014982:Xenopus laevis / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D051846:beta-Synuclein / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D029867:Xenopus Proteins; D051846:beta-Synuclein