title: Use of nanopatterned surfaces to enhance immunoreaction efficiency.
abstract: In this work, we compare the immunoreaction efficiency between uniformly functionalized surface and chemically nanopatterned surfaces when applied as platforms for antigen/antibody interactions with and without the use of protein A as orienting protein. On the nanopatterned platform, the immunoreaction efficiency is higher than all the other cases with no protein A pretreatment of the surface, providing evidence of the capability of the adhesive/antiadhesive nanopatterned surface to immobilize the molecules in a reactive state, increasing their possibility to form complexes.
authors.affiliation: European Commission Joint Research Centre, Institute for Health and Consumer Protection, 21020 Ispra (Varese), Italy.
authors.forename: A
authors.initials: A
authors.lastname: Valsesia
authors.forename: P
authors.initials: P
authors.lastname: Colpo
authors.forename: I
authors.initials: I
authors.lastname: Mannelli
authors.forename: S
authors.initials: S
authors.lastname: Mornet
authors.forename: F
authors.initials: F
authors.lastname: Bretagnol
authors.forename: G
authors.initials: G
authors.lastname: Ceccone
authors.forename: F
authors.initials: F
authors.lastname: Rossi
mesh_terms: D000941:Antigens / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000737:chemistry; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry*; D013205:Staphylococcal Protein A / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A