The dosage of chromatin proteins affects transcriptional silencing and DNA repair in Saccharomyces cerevisiae.
Alterations in protein composition or dosage within chromatin may trigger changes in processes such as gene expression and DNA repair. Through transposon mutagenesis and targeted gene deletions in haploids and diploids of Saccharomyces cerevisiae, we identified mutations that affect telomeric silencing in genes encoding telomere-associated Sir4p and Yku80p and chromatin remodeling ATPases Ies2p and Rsc1p. We found that sir4/SIR4 heterozygous diploids efficiently silence the mating type locus HMR but not telomeres, and diploids heterozygous for yku80 and ies2 mutations are inefficient at DNA repair. In contrast, strains heterozygous for most chromatin remodeling ATPase mutations retain wild-type silencing and DNA repair levels. Thus, in diploids, chromatin structures required for DNA repair and telomeric silencing are sensitive to dosage changes.
Benbow
Sarah Z
SZ
Seattle University, Department of Biology, 901 12th Avenue, Seattle, WA 98122, USA.
DuBois
Michelle L
ML
D002843:Chromatin / Q000378:metabolism*; D004260:DNA Repair; D018628:Gene Dosage; D020868:Gene Silencing; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D002843:Chromatin