Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis).
Cold acclimation in conifers is a complex process, the timing and extent of which reflects local adaptation and varies widely along latitudinal gradients for many temperate and boreal tree species. Despite their ecological and economic importance, little is known about the global changes in gene expression that accompany autumn cold acclimation in conifers. Using three populations of Sitka spruce (Picea sitchensis) spanning the species range, and a Picea cDNA microarray with 21,840 unique elements, within- and among-population gene expression was monitored during the autumn. Microarray data were validated for selected genes using real-time PCR. Similar numbers of genes were significantly twofold upregulated (1257) and downregulated (967) between late summer and early winter. Among those upregulated were dehydrins, pathogenesis-related/antifreeze genes, carbohydrate and lipid metabolism genes, and genes involved in signal transduction and transcriptional regulation. Among-population microarray hybridizations at early and late autumn time points revealed substantial variation in the autumn transcriptome, some of which may reflect local adaptation. These results demonstrate the complexity of cold acclimation in conifers, highlight similarities and differences to cold tolerance in annual plants, and provide a solid foundation for functional and genetic studies of this important adaptive process.
Holliday
Jason A
JA
Department of Forest Sciences, University of British Columbia.
Ralph
Steven G
SG
Michael Smith Laboratories, University of British Columbia.
White
Richard
R
Department of Statistics, University of British Columbia.
Bohlmann
Jörg
J
Department of Forest Sciences, University of British Columbia.|Michael Smith Laboratories, University of British Columbia.|Department of Botany, University of British Columbia, BC, Canada V6T 1Z3.
Aitken
Sally N
SN
Department of Forest Sciences, University of British Columbia.
D000064:Acclimatization / Q000235:genetics*; D003080:Cold Temperature; D015870:Gene Expression; D020869:Gene Expression Profiling; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D028222:Picea / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018515:Plant Leaves / Q000378:metabolism / Q000502:physiology; D016133:Polymerase Chain Reaction; D012621:Seasons; D036226:Seedlings / Q000378:metabolism / Q000502:physiology; D013997:Time Factors