Chitosan biotinylation and electrodeposition for selective protein assembly.
An alternative route to protein assembly at surfaces based on using the unique capabilities of biological materials for the spatially selective assembly of proteins is described. Specifically, the stimuli-responsive properties of aminopolysaccharide chitosan are combined with the molecular-recognition capabilities of biotin-streptavidin binding. Biotinylated chitosan retains its stimuli-responsive properties and is capable of electrodepositing at specific electrode addresses. Once deposited, it is capable of binding streptavidin, which can mediate the subsequent assembly of biotinylated proteins. Spatially selective protein assembly using biotinylated Protein A and fluorescently-labeled antibodies is demonstrated.
Shi
Xiao-Wen
XW
Center for Biosystems Research, University of Maryland Biotechnology Institute, 5115 Plant Sciences Building, College Park, MD 20742, USA.
Liu
Yi
Y
Lewandowski
Angela T
AT
Wu
Li-Qun
LQ
Wu
Hsuan-Chen
HC
Ghodssi
Reza
R
Rubloff
Gary W
GW
Bentley
William E
WE
Payne
Gregory F
GF
D000906:Antibodies; D001360:Avidin; D019921:Biotinylation; D048271:Chitosan / Q000737:chemistry*; D004595:Electroplating; D011506:Proteins / Q000737:chemistry*; D013205:Staphylococcal Protein A
D000906:Antibodies; D011506:Proteins; D013205:Staphylococcal Protein A; D001360:Avidin; D048271:Chitosan