High-density mapping of single-molecule trajectories with photoactivated localization microscopy.
We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.
Manley
Suliana
S
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Gillette
Jennifer M
JM
Patterson
George H
GH
Shroff
Hari
H
Hess
Harald F
HF
Betzig
Eric
E
Lippincott-Schwartz
Jennifer
J
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D007089:Image Enhancement / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence / Q000379:methods*
D008565:Membrane Proteins