title: Renal transplantation normalized hydrogen peroxide production of neutrophils within the first day.
abstract: BACKGROUND
  Hemodialysis patients are in a state of oxidant stress. In renal transplantation reactive oxygen species (ROS) are considered to be important factors of ischemia-reperfusion injury. Neutrophils produce ROS as part of the host defense against invading bacteria. This study was designed to investigate whether neutrophil function in hemodialysis patients is immediately affected by renal transplantation.
  
  
  METHODS
  We evaluated the neutrophil respiratory burst and phagocytic activity in renal transplant patients with living-related donor (LRD) and cadaveric donor (CAD) grafts using flow cytometry techniques. Twenty patients (LRD = 6, CAD = 14) and 20 healthy volunteers were included in the study. Venous blood samples were drawn before anesthesia, 5 min before reperfusion, 1 h and 1, 3 and 7 days after reperfusion.
  
  
  RESULTS
  Before surgery, a significant increase in hydrogen peroxide production in neutrophils was seen for both renal transplantation groups compared to healthy subjects. Within 24 h after reperfusion hydrogen peroxide production almost decreased to normal values. The phagocytic capacity of neutrophils was continuously depressed. There were no differences between the CAD and LRD groups.
  
  
  CONCLUSIONS
  We found that the enhanced respiratory burst activity of patients with chronic renal failure decreased to normal values within 1 day following renal transplantation. Our results suggest that reduced respiratory burst activity resulting in a diminished risk of tissue damage by the uncontrolled production of ROS.
authors.affiliation: Department of Anesthesiology, Medical School Hannover, Hannover, Germany. juettner.bjoern@mh-hannover.de
authors.forename: Björn
authors.initials: B
authors.lastname: Jüttner
authors.forename: Axel
authors.initials: A
authors.lastname: Gehrmann
authors.forename: Dirk
authors.initials: D
authors.lastname: Breitmeier
authors.forename: Karsten
authors.initials: K
authors.lastname: Jaeger
authors.forename: Annette
authors.initials: A
authors.lastname: Weissig
authors.forename: Albrecht
authors.initials: A
authors.lastname: Bornscheuer
authors.forename: Siegfried
authors.initials: S
authors.lastname: Piepenbrock
authors.forename: Dirk
authors.initials: D
authors.lastname: Scheinichen
mesh_terms: D000328:Adult; D002102:Cadaver; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000378:metabolism; D016030:Kidney Transplantation; D019520:Living Donors; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism; D006435:Renal Dialysis; D016897:Respiratory Burst / Q000502:physiology; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide