title: Isolation and partial characterization of A beta-glucuronidase of the mollusk Pomacea sp.
abstract: This paper studies the beta-glucuronidase in the mollusk Pomacea sp. The beta-glucuronidase was isolated 206-fold with a 1,5% yield and the cinetc parameters was: pH 5.0, 65 degrees C, K(m) of 72 x 10(-2) mM and molecular mass of 116 kDa. HPLC confirmed the purity. BaCl(2) increased beta-glucuronidase activity and SDS and NaH(2)PO(4) inhibited completely.
authors.affiliation: Department of Biochemistry, Center for Biosciences, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
authors.forename: Maria Lúcia Lira
authors.initials: ML
authors.lastname: de Andrade
authors.forename: Vanessa Duarte
authors.initials: VD
authors.lastname: de Morais
authors.forename: João Felipe
authors.initials: JF
authors.lastname: de Sousa Filho
mesh_terms: D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000378:metabolism; D049851:Gastropoda / Q000201:enzymology*; D005966:Glucuronidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D012995:Solubility; D013696:Temperature; D013997:Time Factors
chemical_list: D002809:Chondroitin Sulfates; D005966:Glucuronidase