Quantitative determination of secondary metabolites in Cladina stellaris and other lichens by micellar electrokinetic chromatography.
The quantitative determination of usnic acid (UA), perlatolic acid (PA), and atranorin (AT) in Alaska lichens by micellar electrokinetic chromatography (MEKC) is reported. The background electrolyte (BGE) included sodium docecyl sulfate (SDS), and beta-cyclodextrin (beta-CD) in a high-pH borate buffer. The presence of beta-CD in the buffer significantly decreases peak width, especially for UA, as it decreases migration time for both UA and PA. Linear calibration curves for UA, PA, and AT were established using an internal standard of benzoic acid (BA). Concentration limits of detection (cLODs) are 2.5, 2.2 and 2.0microg/mL (S/N 3) for UA, PA, and AT, respectively. Dry samples of lichen were extracted at room temperature with acetone for 24h in the presence of BA as internal standard. Recoveries of UA from spiked samples ranged from 92 to 98%. Amounts of UA and PA in the lichen samples ranged from 0.28 to 1.7% dry weight and 0.02 to 0.23%, respectively.
Falk
Adelia
A
Department of Biology and Wildlife, University of Alaska Fairbanks, Fairbanks, AK 99775, USA.
Green
Thomas K
TK
Barboza
Perry
P
D001565:Benzoates / Q000032:analysis / Q000737:chemistry; D001572:Benzofurans / Q000032:analysis / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D062385:Hydroxybenzoates / Q000032:analysis / Q000737:chemistry; D008011:Lichens / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results
D001565:Benzoates; D001572:Benzofurans; D062385:Hydroxybenzoates; C522034:perlatolic acid; C073339:usnic acid; C026304:atranorin