Overnight or fresh buffy coat-derived platelet concentrates prepared with various platelet pooling systems.
BACKGROUND
For logistic reasons, possibilities to produce both platelet (PLT) concentrates prepared from fresh or overnight-stored whole blood (fresh and o/n PCs, respectively) are convenient. The consequences of both possibilities are not well described. The PLT pooling system used might also influence the condition of PCs. Our aim was to compare fresh and o/n PCs with different PLT pooling systems.


METHODS
Fresh and o/n PCs were prepared from buffy coats and plasma in PLT pooling systems of Baxter, Fresenius, Terumo, or Pall (n = 5). PCs were stored for 9 days. The in vitro quality was determined by the PLT count, pH, glucose, lactate, pO(2), pCO(2), CD62P expression, and annexin V binding.


RESULTS
The o/n PCs showed higher PLT count (approx. 460 x 10(9)/PC vs. approx. 310 x 10(9)/PC), pCO(2), and lactate concentration and lower pH, pO(2), glucose concentration, CD62P expression (until Day 5), and annexin V binding (until Day 7) compared with fresh PCs (p < 0.05). Only for o/n PCs in the Baxter and Fresenius systems did the pH and glucose concentration remain higher, and the lactate concentration and CD62P expression remained lower than that of o/n PCs in the Pall and Terumo systems. The pH for fresh PCs in the Baxter and Fresenius systems was more often greater than 7.4 than for fresh PCs in the Terumo or Pall systems.


CONCLUSIONS
The quality of PCs depended on whether PCs were prepared from fresh or overnight-stored whole blood and on the used PLT pooling system. The main difference between fresh and o/n PCs was the PLT count.
Dijkstra-Tiekstra
Margriet J
MJ
Sanquin Blood Bank Northeast Region, Groningen, The Netherlands. m.dijkstra@sanquin.nl
Kuipers
Willeke
W
Setroikromo
Airies C
AC
de Wildt-Eggen
Janny
J
D017304:Annexin A5 / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D010976:Platelet Count; D011485:Protein Binding
D017304:Annexin A5; D019007:P-Selectin; D019344:Lactic Acid; D005947:Glucose