Expression and prognostic relevance of annexin A3 in prostate cancer.
OBJECTIVE
By differential quantitative protein expression, it has previously been shown that annexin A3 (ANXA3) expression is associated with prostate cancer. However little is known about the role and biology of ANXA3 in the human prostate. The aim of this study was to thoroughly analyze ANXA3 expression patterns and its potential as a prognostic marker in a large set of benign, preneoplastic, and neoplastic prostate tissue samples.


METHODS
Immunohistochemistry-based ANXA3 protein expression was analyzed for 1589 prostate cancers as well as smaller subsets of benign epithelium and high-grade prostatic intraepithelial neoplasia (PIN) in a tissue microarray format.


RESULTS
All samples of benign prostatic epithelium and PIN showed ANXA3 protein expression, with PIN lesions showing a decreased staining intensity compared with benign epithelium (p<0.0001). In cancer, ANXA3 protein expression was essentially reduced, resulting in a negative staining rate of 27.2%, which correlated with increasing pT stage and Gleason score (p<0.0001). ANXA3 status in cancer was shown to be an independent adverse prognostic factor and enabled substratification of the large group of intermediate-risk patients (n=969) into high- and low-risk subgroups.


CONCLUSIONS
ANXA3 represents a promising candidate tissue marker, and when combined with the standard prognostic parameters, is suggested to provide a more precise prediction of prognosis in the individual patient, therefore harboring the potential to contribute to future patient management.
Köllermann
Jens
J
Institute of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Schlomm
Thorsten
T
Bang
Holger
H
Schwall
Gerhard P
GP
von Eichel-Streiber
Christoph
C
Simon
Ronald
R
Schostak
Martin
M
Huland
Hartwig
H
Berg
Wigbert
W
Sauter
Guido
G
Klocker
Helmut
H
Schrattenholz
André
A
D000368:Aged; D017318:Annexin A3 / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology
D017318:Annexin A3