DDT- and DDE-induced disruption of ovarian steroidogenesis in prepubertal porcine ovarian follicles: a possible interaction with the main steroidogenic enzymes and estrogen receptor beta.
We evaluated impact of DDT isomers, o, p'- DDT [1, 1-dichloro-2, 2-bis (p, p'-chlorophenyl) ethylene] and p, p'-DDT [1, 1, 1-trichloro-2, 2-bis (p-chlorophenyl) ethane], and their metabolites, o, p'-DDE and p, p'-DDE, on ovarian steroidogenesis. All these compounds, except for p, p'-DDT, demonstrated estrogenic effects on steroid secretion in co-cultures of porcine prepubertal granulosa and theca cells. p,p'-DDT decreased progesterone and estradiol release, which was reversed by the addition of testosterone. In contrast, o, p'-DDT inhibited progesterone secretion with parallel stimulation of basal and testosterone-stimulated estradiol release. DDEs stimulated progesterone and estradiol secretion. The fluorometric assay confirmed that p,p'-DDE, o,p'-DDT, and o,p'-DDE stimulated aromatase activity. Western blots indicated that o,p-DDT and o,p'-DDE diminished the expression of estrogen receptor beta (ERbeta). This study demonstrated the isomer-dependent action of DDT in pig ovarian cells. We propose that DDT could disrupt ovarian steroidogenesis either by interfering with main steroidogenic enzymes or affecting ERbeta.
Wójtowicz
A K
AK
Laboratory of Physiology and Toxicology of Reproduction, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, Poland. wojtow@zuk.iz.uj.edu.pl.
Kajta
M
M
Gregoraszczuk
E Ł
EŁ
D000818:Animals; D001141:Aromatase / Q000378:metabolism*; D002478:Cells, Cultured; D003634:DDT / Q000378:metabolism / Q000633:toxicity*; D003633:Dichlorodiphenyl Dichloroethylene / Q000378:metabolism / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000096:biosynthesis; D047629:Estrogen Receptor beta / Q000096:biosynthesis*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D007536:Isomerism; D008939:Mitotane / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D010575:Pesticides / Q000378:metabolism / Q000633:toxicity; D011374:Progesterone / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis*; D013552:Swine; D013799:Theca Cells / Q000187:drug effects / Q000378:metabolism
D047629:Estrogen Receptor beta; D010575:Pesticides; D013256:Steroids; D011374:Progesterone; D003633:Dichlorodiphenyl Dichloroethylene; D004958:Estradiol; D008939:Mitotane; D003634:DDT; C016340:o,p'-DDT; C054919:2,2-(2-chlorophenyl-4'-chlorophenyl)-1,1-dichloroethene; D001141:Aromatase