[Effects of traditional Chinese medicine on oral bacteria biofilm].
OBJECTIVE
To investigate the effects of compounds of Galla chinensis extract (GCE) and Nidus vespae extract-1 (WVE1) on oral bacteria biofilm structure and activity and to determine the possibility of caries prevention by the compounds.


METHODS
The morphology and activity of treated-oral bacterial biofilm and untreated-oral bacterial biofilm were observed by using fluorescence microscope in combination of idio-fluorochrome to label the died and living bacteria. The visible light semiquantitative method was used to measure biomass glucosyltransferase (GTF, A620) values and to determine the effects of active compounds of GCE and NVE1 on GTF of oral bacteria biofilm.


RESULTS
The living bacteria in the untreated 24 h bacterial biofilm was dominant, and only a small number of died bacteria were found, the biofilm structure was regular and clear. GCE, GCE-B and NVE1 could inhibit the bacteria in the dental biofilm, which showed significant difference with the negative control. GCE and NVE1 could also inhibit GTF activity of 24 h bacterial biofilm in comparison with the negative control.


CONCLUSIONS
The traditional Chinese medicine Galla chinensis and Nidus vespae could not only inhibit bacteria growth on oral bacterial biofilm, but also function by adjusting biofilm structure, composition and GTF activity of 24 h bacterial biofilm.
Zhao
Jin
J
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, Sichuan University, Chengdu 610041, China.
Li
Ji-yao
JY
Zhu
Bing
B
Zhou
Xue-dong
XD
D016924:Actinomyces viscosus / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D001431:Bacteriological Techniques; D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000382:microbiology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005964:Glucosyltransferases / Q000378:metabolism*; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D013298:Streptococcus sanguis / Q000187:drug effects / Q000201:enzymology / Q000502:physiology
D004365:Drugs, Chinese Herbal; D005964:Glucosyltransferases