title: Recognition of Staphylococcus enterotoxin via molecularly imprinted beads.
abstract: The synthesis of molecularly imprinted beads for the recognition of the protein Staphylococcus enterotoxin B (SEB) is described. Two kinds of organic silane (3-aminopropyltrimethoxysilane (APTMS) and octyltrimethoxysilane (OTMS)) were polymerized on the surface of polystyrene microspheres after the SEB template was covalently immobilized by forming imine bonds. The resulting imprinted beads were selective for SEB. The Langmuir adsorption models were applied to describe the equilibrium isotherms. The results showed that an equal class of adsorption was formed in the molecularly imprinted polymer (MIP) with the maximum adsorption capacity of 3.86 mg SEB/g imprinted beads. The MIP has much higher adsorption capacity for SEB than the nonimprinted polymer, and the MIP beads have a higher selectivity for the template molecule.
authors.affiliation: Institute of Hygienic and Environmental Medicine, Tianjin, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.forename: Baoan
authors.initials: B
authors.lastname: Ning
authors.forename: Huanying
authors.initials: H
authors.lastname: Zhou
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Fang
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000327:Adsorption; D001427:Bacterial Toxins / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011108:Polymers; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013210:Staphylococcus / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D011108:Polymers