[Experimental study of maxillary sinus lifting with tissue engineered bone].
OBJECTIVE
To evaluate the effect of tissue engineering bone in maxillary sinus lifting.


METHODS
The marrow stromal stem cells of dog were cultured in DMEM containing 100 m1/L fetal bovine serum and induced to differentiate to osteoblasts, which were then inoculated together with Bio-Oss for 5 days. Sixteen dog's bilateral maxillary sinus were elevated. One side was grafted with a compound of BMSC and Bio-Oss and the other side grafted with Bio-Oss alone. The samples were studied by gross, CT, histomorphology and histomorphometrical analysis at the 30th, 90th day after the operation. t-test was used for statistical analysis.


RESULTS
In gross view and CT, new bone formation was observed in all maxillary sinus after 30 and 90 days respectively. Histomorphometrical analysis showed much more new callus in BMSC-Bio-Oss group than in Bio-Oss group (P < 0.05).


CONCLUSIONS
A better effect of new bone formation could be obtained with tissue engineered bone in maxillary sinus lifting.
Chen
Xiao-li
XL
Department of Stomatology, Zhongshan Hospital, Xiamen University, Xiamen Fujian 361004, China. chxiaol3@hotmail.com
Li
Zhong-lin
ZL
Zhang
Wen-bin
WB
Ye
Zhan-chao
ZC
Ke
Shu-nü
SN
D000818:Animals; D018786:Bone Substitutes; D002478:Cells, Cultured; D004285:Dogs; D008443:Maxillary Sinus / Q000601:surgery*; D008903:Minerals; D023461:Myeloid Progenitor Cells / Q000637:transplantation*; D023822:Tissue Engineering; D054457:Tissue Scaffolds
C077540:Bio-Oss; D018786:Bone Substitutes; D008903:Minerals