Protective effect of tetramethylpyrazine and salvianolic acid B on apoptosis of rat cerebral microvascular endothelial cell under high shear stress.
Apoptosis induced by high shear stress has been reported for the dysfunction of various vascular endothelial cells. We investigated the protective effects of tetramethylpyrazine (TMP) and salvianolic acid B (SAB) from Chinese medicine on the shear-induced early and late stages of apoptosis in cultured rat cerebral microvascular endothelial cells (rCMECs) under pathological high shear stress. Near-confluent cultures of rCMECs were pretreated with TMP or SAB and their combinational dosages, and exposed to high shear stress generated by a rheometer. Apoptotic death rate of rCMECs was assessed by immunofluorescence microscopy of Annexin V-FITC and propidium iodide (PI). We found that early and late stage apoptosis occurred at 3.0 Pa for a short duration of 450 sec but did not occur at 1.5 Pa. SAB inhibited the cells from apoptosis at concentrations from 10 microM to 20 microM in a dose-dependent manner, while effect of TMP at 0.37 mM and 0.73 mM did not significantly differ. Moreover, the combined use of TMP and SAB had synergistic anti-apoptotic effects (P<0.01). The results indicate that the anti-apoptotic effect of TMP and SAB on rheologically induced endothelial injury is likely involved in their efficacy.
Li
Min
M
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong. limin@hkbu.edu.hk
Zhao
Ming-Qi
MQ
Kumar Durairajan
Siva Sundara
SS
Xie
Li-Xia
LX
Zhang
Hong-Xia
HX
Kum
Wan-Fung
WF
Goto
Shinya
S
Liao
Fu-Long
FL
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001572:Benzofurans / Q000494:pharmacology*; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D004365:Drugs, Chinese Herbal; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018056:Hemorheology / Q000379:methods; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000502:physiology*; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D053630:Depsides
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D011719:Pyrazines; C076944:salvianolic acid B; D053630:Depsides; C017953:tetramethylpyrazine