Comparative proteome analysis of differentially expressed proteins induced by Al toxicity in soybean.
Phytotoxic aluminum (Al) is a limiting factor for crop production on acid soils. The molecular mechanism, however, underlying Al toxicity and responses in plants is still not well understood. We report here the characterization of comparative proteome of aluminum-stress-responsive proteins in a known Al-resistant soybean cultivar, Baxi 10 (BX10). To investigate time-dependent responses, 1-week-old soybean seedlings were exposed to 50 microM AlCl3 for 24, 48 and 72 h, and total proteins extracted from roots were separated by two-dimensional electrophoresis. More than 1200 root proteins of the soybean BX10 seedling were reproducibly resolved on the gels. A total of 39 differentially expressed spots in abundance were identified by mass spectrometry, with 21 upregulated, 13 newly induced and 5 downregulated. The heat shock protein, glutathione S-transferase, chalcone-related synthetase, GTP-binding protein and ABC transporter ATP-binding protein were previously detected at the transcriptional or translational level in other plants. Other proteins, identified in this study, are new Al-induced proteins. Soybean BX10 roots under aluminum stress could be characterized by the cellular activities involved in stress/defense, signal transduction, transport, protein folding, gene regulation, and primary metabolisms, which are critical for plant survival under Al toxicity. This present study expands our understanding of differentially expressed proteins associated with aluminum stress on soybean BX10.
Zhen
Yan
Y
Institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
Qi
Jin-Liang
JL
Wang
Si-Si
SS
Su
Jing
J
Xu
Guo-Hua
GH
Zhang
Ming-Sheng
MS
Miao
Lv
L
Peng
Xin-Xiang
XX
Tian
Dacheng
D
Yang
Yong-Hua
YH
D000535:Aluminum / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020543:Proteome; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000187:drug effects / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010940:Plant Proteins; D020543:Proteome; D000535:Aluminum