[The nature of different influence of Cd2+ ions on the conformation of three-stranded polyU-polyA-polyU and polyI-polyA-polyI in aqueous solution].
The methods of UV (DUV) spectroscopy and thermal denaturation were used to study the effect of Cd2+ ions on the conformational equilibrium of three-stranded (A21, A2U) and single-stranded (poly U, poly A and poly I) polynucleotides in aqueous solutions containing 0.1 M Na+ (pH 7). An analysis of the form and intensities of DUV-spectra of poly A, poly I and A2I revealed the presence of two types of complexes: interaction with N7 of purines, resulting in the formation of macrochelates and binding to N1 of poly A and poly I. Cd2+ ions do not bind to heteroatoms of A2U nitrogen bases, and, therefore, the conformation of its structure remains unchanged up to a concentration of Cd2+ 0.01 M. A "critical" concentration (1.5x10(-4) M) of Cd2+ ions exists above which A2I transits cooperatively into a new helical conformation, which has a lower thermostability. It is supposed that, during the formation of metallized A2I, Cd2+ ions form bridges between the adenine and hypoxanthine of its homopolynucleotide circuits, being arranged inside the triple helix.
Sorokin
V A
VA
Valeev
V A
VA
Usenko
E L
EL
Andrus'
E A
EA
Blagoĭ
Iu P
IuP
D002104:Cadmium / Q000737:chemistry*; D002413:Cations, Divalent / Q000737:chemistry; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000737:chemistry*; D011069:Poly I / Q000737:chemistry*; D011072:Poly U / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D002413:Cations, Divalent; D002104:Cadmium; D014867:Water; D011061:Poly A; D011069:Poly I; D011072:Poly U