title: MASPIN subcellular localization and expression in oral cavity squamous cell carcinoma.
abstract: MASPIN, a member of serpin superfamily, has multifaceted biological functions and an unique tumour suppressing activity. Experimental evidences showed that MASPIN suppresses tumour growth, angiogenesis, invasion and metastasis. Only a very limited number of studies considered MASPIN expression in the upper aero-digestive tract carcinomas. It was recently found that nuclear localization of MASPIN was significantly associated with lower recurrence rate and longer disease-free interval in laryngeal carcinoma. The present study investigated the biological and prognostic role of MASPIN in relation to its subcellular localization in oral carcinoma. Sub-cellular pattern of distribution of MASPIN, nuclear and cytoplasmic MASPIN expressions were immunohistochemically determined in 56 consecutive cases of oral carcinoma. Statistical analysis found a significant association between pN-stage and recurrence of disease (P=0.032) and a significantly longer disease-free interval in pN0 patients than in pN+ ones (P=0.038). None of the subcellular expressions of MASPIN was significantly correlated with recurrence of disease and disease-free interval in our series of oral carcinomas. Sixty-one percent of pN0 cases was strongly MASPIN-positive in the cytoplasm of primary carcinoma cells, 33% of the pN+ cases was MASPIN-positive in the cytoplasm. Statistical analysis found a significant association between MASPIN cytoplasmic expression and pN-stage (P=0.032). Negative MASPIN immunoreactivity in carcinoma cells cytoplasm may be useful to identify patients at risk of disease disseminating to neck lymph nodes. Further investigations are necessary to understand the biological role of cytoplasmic MASPIN localization in oral carcinoma.
authors.affiliation: Department of Medical and Surgical Specialties, Section of Otolaryngology, University of Padova, Via Giustiniani 2, 35100 Padova, Italy. gino.marioni@unipd.it
authors.forename: Gino
authors.initials: G
authors.lastname: Marioni
authors.forename: Elena
authors.initials: E
authors.lastname: Gaio
authors.forename: Luciano
authors.initials: L
authors.lastname: Giacomelli
authors.forename: Andy
authors.initials: A
authors.lastname: Bertolin
authors.forename: Emiliano
authors.initials: E
authors.lastname: D'Alessandro
authors.forename: Roberto
authors.initials: R
authors.lastname: Stramare
authors.forename: Elisa
authors.initials: E
authors.lastname: Facco
authors.forename: Alberto
authors.initials: A
authors.lastname: Staffieri
authors.forename: Stella
authors.initials: S
authors.lastname: Blandamura
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002610:Cheek; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008048:Lip Neoplasms / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local / Q000378:metabolism; D010157:Palatal Neoplasms / Q000378:metabolism; D021362:Palate, Hard; D011379:Prognosis; D015843:Serpins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014062:Tongue Neoplasms / Q000378:metabolism
chemical_list: C085344:SERPIN-B5; D015843:Serpins