title: Preparation of mannan modified anionic PCL-PEG-PCL nanoparticles at one-step for bFGF antigen delivery to improve humoral immunity.
abstract: In this article, blank anionic poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCEC) and anionic mannan modified PCEC (MPCEC) nanoparticles with nearly the same particle size and zeta potential were prepared by emulsion solvent evaporation method. Human basic fibroblast growth factor (bFGF) was absorbed onto anionic nanoparticles surface due to electrostatic interaction. The obtained bFGF-nanoparticles complexes were injected subcutaneously into C57BL/6 mice at 20 microg of bFGF/dose on week 0, 1, 2 and 3. The mice serum was collected on week 4, and bFGF-specific autoantibody total IgG, IgG1 and IgG2a titer in serum was determined by ELISA. The results indicated that the autoantibody IgG, IgG1 and IgG2a titer of the mice immunized by bFGF-MPCEC complexes were higher than that immunized by either bFGF-PCEC or bFGF-Alum. This phenomenon might be due to that mannan functionalized MPCEC nanoparticles could be targeted to dendritic cells (DCs) to improve humoral immunity. The prepared anionic mannan modified PCEC nanoparticles (MPCEC) might have great potential application in vaccine delivery systems.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Maling
authors.initials: M
authors.lastname: Gou
authors.forename: Mei
authors.initials: M
authors.lastname: Dai
authors.forename: XingYi
authors.initials: X
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: MeiJuan
authors.initials: M
authors.lastname: Huang
authors.forename: YongShen
authors.initials: Y
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Kan
authors.forename: You
authors.initials: Y
authors.lastname: Lu
authors.forename: YuQuan
authors.initials: Y
authors.lastname: Wei
authors.forename: ZhiYong
authors.initials: Z
authors.lastname: Qian
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D016503:Drug Delivery Systems; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007279:Injections, Subcutaneous; D008351:Mannans; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D011091:Polyesters / Q000138:chemical synthesis; D011092:Polyethylene Glycols / Q000138:chemical synthesis
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D008351:Mannans; D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols