title: Immune function abnormalities in peripheral blood mononuclear cell cytokine expression differentiates stages of cutaneous T-cell lymphoma/mycosis fungoides.
abstract: OBJECTIVE
  Mycosis fungoides (MF) is a cutaneous T-cell lymphoma (CTCL) characterized by neoplastic skin-homing T cells. To better understand the immunopathogenesis of MF, we analyzed the functional ability of peripheral blood mononuclear cells (PBMC) from early and late MF/CTCL patients to express cytokine genes. In late stage MF/CTCL, patients were separated into those with blood involvement (+B) and without blood involvement (-B).
  
  
  METHODS
  We analyzed T(H)1 (interleukin 2 (IL-2), IFN-gamma), T(H)2 (IL-4, IL-5, IL-10, IL-13), and T(H)17 (IL-17) cytokine gene expression from activated PBMCs from normal (n = 12), psoriasis (n = 6), early MF/CTCL (n = 11), and late MF/CTCL+B (n = 4) and MF/CTCL-B (n = 3) by quantitative real-time PCR.
  
  
  RESULTS
  PBMCs from early MF/CTCL and psoriasis showed higher induction of IL-2, IL-4, and IFN-gamma genes than those from normal and late MF/CTCL-B and MF/CTCL+B (P < 0.05) in descending order. PBMCs from late MF/CTCL-B exhibited generally the highest level of IL-5, IL-10, IL-13, and IL-17 expression compared with the other groups. PBMCs from early MF/CTCL and late MF/CTCL-B had similarly elevated IL-13 and IL-17. Of all groups, PBMCs from late MF/CTCL+B had the lowest levels of IL-2 (P < 0.05), IL-4, IFN-gamma, IL-13, and IL-17.
  
  
  CONCLUSIONS
  The different pattern of cytokine gene expression suggests a change in immune function in MF/CTCL from early MF/CTCL to late MF/CTCL-B to late MF/CTCL+B. These stages are consistent with localized disease associated with an anti-tumor immune response and late MF/CTCL associated with a loss of immune function mediated by malignant T cells that share regulatory T cell-like properties.
authors.affiliation: Department of Dermatology, Henry Ford Hospital, Detroit, Michigan 48202, USA.
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Chong
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Wilson
authors.forename: Heather M
authors.initials: HM
authors.lastname: Gibson
authors.forename: Mikehl S
authors.initials: MS
authors.lastname: Hafner
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.forename: Carrie J
authors.initials: CJ
authors.lastname: Hedgcock
authors.forename: Henry K
authors.initials: HK
authors.lastname: Wong
mesh_terms: D016207:Cytokines / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007378:Interleukins / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000276:immunology*; D009182:Mycosis Fungoides / Q000235:genetics* / Q000276:immunology*; D011565:Psoriasis / Q000235:genetics / Q000276:immunology; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology; D012878:Skin Neoplasms / Q000235:genetics* / Q000276:immunology*
chemical_list: D016207:Cytokines; D007378:Interleukins; D012334:RNA, Neoplasm