title: Cotranslational translocation of proteins into canine rough microsomes.
abstract: Rough microsomes (RMs) are small vesicular fragments of the rough endoplasmic reticulum (ER). They can be used in cell-free protein translocation studies to analyze ER targeting signals, signal sequence cleavage, N-linked glycosylation, and membrane protein biogenesis. This unit describes preparation and use of RMs. Rough microsomes (RMs) are small vesicular fragments of the rough endoplasmic reticulum (ER). They can be used in cell-free protein translocation studies to analyze ER targeting signals, signal sequence cleavage, N-linked glycosylation, and membrane protein biogenesis. This unit describes preparation and use of RMs.
authors.affiliation: National Institute of Diabetes and Digestive and Kidney Disesases/NIH, Bethesda, Maryland, USA.
authors.forename: H D
authors.initials: HD
authors.lastname: Bernstein
mesh_terms: D000818:Animals; D002474:Cell-Free System; D003584:Cytological Techniques; D004285:Dogs; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010179:Pancreas / Q000166:cytology; D021381:Protein Transport / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins