Variance in multiplex suspension array assays: a distribution generation machine for multiplex counts.
BACKGROUND
This study attempted to replicate Luminex experimental results for large numbers of beads per classifier using multiplexed assays and routine instrument use conditions.


CONCLUSIONS
Using larger numbers of microspheres per classifier highlights a fundamental stochastic distribution of bead counts issue complicated by other factors. The more classifiers and the higher the count required per classifier there are, the more apparent the distribution of counts per classifier will be, and the more microspheres are required. Additional problems have been identified. Alternate methods of improving precision and reliability are recommended such as intraplexing and multi-well sample replicates to improve precision and confidence.
Hanley
Brian P
BP
Microbiology Graduate Group, University of California, Davis, CA 95616, USA. bphanley@ucdavis.edu
D000906:Antibodies; D000941:Antigens; D005434:Flow Cytometry / Q000379:methods*; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods*; D008863:Microspheres; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000906:Antibodies; D000941:Antigens