Chromosomal localization of silkworm (Bombyx mori) sericin gene 1 and chymotrypsin inhibitor 13 using fluorescence in situ hybridization.
The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5+/-1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2+/-1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.
Song
FangZhou
F
Department of Molecular Biology, Chongqing Medical University, Chongqing 400016, China. fzsongcq@163.com
Chang
PingAn
P
Zhang
PingBo
P
Yi
FaPing
F
Ma
YongPing
Y
Lu
Cheng
C
Banno
Yutaka
Y
Fujii
Hiroshi
H
D000818:Animals; D012831:Bombyx / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D002875:Chromosomes / Q000201:enzymology / Q000235:genetics; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008957:Models, Genetic; D047030:Sericins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D015842:Serine Proteinase Inhibitors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
D019476:Insect Proteins; D047030:Sericins; D015842:Serine Proteinase Inhibitors; C528829:chymotrypsin inhibitor 13 protein, Bombyx mori; D002918:Chymotrypsin