title: [Effect of Betamethasone on the cAMP of olfactory receptor neurons in rat].
abstract: OBJECTIVE
  To observe the effects of Betamethasone on the cyclic adenosine monophosphate (cAMP) in olfactory receptor neurons (ORNs) and explore the possible mechanisms of the recovery of olfactory disorders by steroid treatment
  
  
  METHODS
  ORNs membrane was extracted and incubated with 0.1 mg/ml and 1.0 mg/ml Betamethasone. The concentrations of cAMP were measured by enzyme-linked immunosorbent assay at different times.
  
  
  RESULTS
  Compared with the control group, all Betamethasone groups showed differences, indicating 0.1 mg/ml (P < 0.05) and 1.0 mg/ml (P < 0.01) Betamethasone could rise the concentration of cAMP. The Betamethasone had obvious effects on cAMP production in rat ORNs and there was a dose-dependent effect. There was no difference between 5 minutes groups and 30 minutes groups.
  
  
  CONCLUSIONS
  Steroid hormone could enhance the production of cAMP of ORNs. Steroid hormone may thus contribute to the recovery of olfactory disorders partially, at least, through the effect on AC-cAMP in olfactory transduction.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital University of Medical Science, Beijing 100730, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Zhang
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Wei
authors.forename: Xu-tao
authors.initials: XT
authors.lastname: Miao
authors.forename: Fei-hong
authors.initials: FH
authors.lastname: Xing
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zhan
mesh_terms: D000818:Animals; D001623:Betamethasone / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D008564:Membrane Potentials; D018034:Olfactory Receptor Neurons / Q000187:drug effects* / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
chemical_list: D001623:Betamethasone; D000242:Cyclic AMP