Effect of monothiol along with antioxidant against mercury-induced oxidative stress in rat.
Efficacy of thiol chelators viz. N-acetyl cysteine and D-penicillamine (NAC and DPA) along with nutritional supplements viz. zinc acetate, sodium selenite and magnesium sulphate (Zn, Se and Mg) in the treatment of mercury intoxication was investigated in rats. This is of particular interest since high bonding affinity between mercuric ion and the thiol group exits. The mutual antagonism of mercury and selenium is one of the strongest examples of the interaction in the trace element field. Adult rats of Sprague-Dawley strain were administered a bolus dose of dimethyl mercury (10 mg/kg) orally. A significant rise in the aspartate aminotransferase, alanine aminotransferase, serum alkaline phosphatase, lactate dehydrogenase, gamma glutamyltranspeptidase, bilirubin and creatinine were observed. Single mercury exposure also resulted in a significant increase in lipid peroxides with a concomitant decrease in reduced glutathione level in liver, kidney and brain. A decrease in the enzymatic activities of acetyl cholinesterase in different regions of the brain was observed. These parameters were restored considerably with chelating agents along with nutritional supplementation, but NAC+Se and DPA+Mg offered significant protection in comparison with other combinations.
Singh
Varsha
V
Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior 474 011, India.
Joshi
Deepmala
D
Shrivastava
Sadhana
S
Shukla
Sangeeta
S
D000111:Acetylcysteine / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002614:Chelating Agents / Q000627:therapeutic use*; D056486:Chemical and Drug Induced Liver Injury; D019587:Dietary Supplements; D004359:Drug Therapy, Combination; D008107:Liver Diseases / Q000188:drug therapy* / Q000473:pathology; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D008630:Mercury Poisoning / Q000188:drug therapy* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010396:Penicillamine / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018038:Sodium Selenite / Q000494:pharmacology*; D016896:Treatment Outcome; D015032:Zinc / Q000494:pharmacology
D000975:Antioxidants; D002614:Chelating Agents; D010396:Penicillamine; D018038:Sodium Selenite; D008274:Magnesium; D015032:Zinc; D000111:Acetylcysteine