title: Silanizing glassware.
abstract: This unit provides a protocol for silanizing (siliconizing) glassware to prevent adsorption of solute to the glass surface and to increase the hydrophobicity of the surface. This is particularly important when dealing with low concentrations of particularly "sticky" solutes such as single-stranded nucleic acids or proteins or cells.
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: B
authors.initials: B
authors.lastname: Seed
mesh_terms: D000327:Adsorption; D005898:Glass / Q000032:analysis / Q000592:standards; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents; D008967:Molecular Biology / Q000295:instrumentation* / Q000379:methods; D013499:Surface Properties; D014297:Trimethylsilyl Compounds
chemical_list: D007202:Indicators and Reagents; D014297:Trimethylsilyl Compounds; C039293:trimethylchlorosilane