title: A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays.
abstract: We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
authors.affiliation: Department of Physics, University of California at Davis, Davis, California 95616, USA.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Fei
authors.forename: J P
authors.initials: JP
authors.lastname: Landry
authors.forename: Y S
authors.initials: YS
authors.lastname: Sun
authors.forename: X D
authors.initials: XD
authors.lastname: Zhu
authors.forename: J T
authors.initials: JT
authors.lastname: Luo
authors.forename: X B
authors.initials: XB
authors.lastname: Wang
authors.forename: K S
authors.initials: KS
authors.lastname: Lam
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012371:Robotics / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D013194:Staining and Labeling