Chemerin enhances insulin signaling and potentiates insulin-stimulated glucose uptake in 3T3-L1 adipocytes.
To explore a novel adipokine, we screened adipocyte differentiation-related gene and found that TIG2/chemerin was strongly induced during the adipocyte differentiation. Chemerin was secreted by the mature 3T3-L1 adipocytes and expressed abundantly in adipose tissue in vivo as recently described. Intriguingly, the expression of chemerin was differently regulated in the liver and adipose tissue in db/db mice. In addition, serum chemerin concentration was decreased in db/db mice. Chemerin and its receptor/ChemR23 were expressed in mature adipocytes, suggesting its function in autocrine/paracrine fashion. Finally, chemerin potentiated insulin-stimulated glucose uptake concomitant with enhanced insulin signaling in the 3T3-L1 adipocytes. These data establish that chemerin is a novel adipokine that regulates adipocyte function.
Takahashi
Michiko
M
Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.
Takahashi
Yutaka
Y
Takahashi
Kenichi
K
Zolotaryov
Fyodor N
FN
Hong
Kyoung Su
KS
Kitazawa
Riko
R
Iida
Keiji
K
Okimura
Yasuhiko
Y
Kaji
Hidesuke
H
Kitazawa
Sohei
S
Kasuga
Masato
M
Chihara
Kazuo
K
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity / Q000187:drug effects; D019556:COS Cells; D002454:Cell Differentiation / Q000187:drug effects; D018925:Chemokines; D002630:Chemotactic Factors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003470:Culture Media; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015398:Signal Transduction / Q000187:drug effects
D018925:Chemokines; D002630:Chemotactic Factors; D003470:Culture Media; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; C523686:chemerin protein, mouse; D005947:Glucose