title: Metabolic labeling with fatty acids.
abstract: Covalent attachment of radiolabeled fatty acids (e.g., [(3)H]myristate or palmitate) is an alternative method for labeling proteins. This unit contains methods for biosynthetic labeling with fatty acids, analysis of the fatty acid linkage with protein, analysis of total protein-bound fatty acid level in cell extracts, and analysis of the identity of the bound fatty acid.
authors.affiliation: National Institute For Medical Research, London, United Kingdom.
authors.forename: C S
authors.initials: CS
authors.lastname: Jackson
authors.forename: A I
authors.initials: AI
authors.lastname: Magee
mesh_terms: D000215:Acylation; D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D019814:Myristic Acid / Q000032:analysis / Q000378:metabolism; D019308:Palmitic Acid / Q000032:analysis / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D015531:Radioimmunoprecipitation Assay / Q000379:methods; D014316:Tritium / Q000032:analysis*
chemical_list: D005227:Fatty Acids; D007202:Indicators and Reagents; D011506:Proteins; D019814:Myristic Acid; D014316:Tritium; D019308:Palmitic Acid