Morphological variation of Enterobacter sp. BL-2 in acetate-mediated pH environment for excretive production of cationic microbial polyglucosamine biopolymer.
Enterobacter sp. BL-2 excretively produced unique cationic polyglucosamine biopolymer PGB-1 comprised of more than 95% D-glucosamine in an acetate-mediated culture condition. The excretion of the biopolymer PGB- was closely associated with the cellular morphology Enterobacter sp. BL-2, a feature highly dependable on the pH of the medium. The initially formed uneven and irregular surface cells were aggregated into the cell-biopolymer network structure connected by the adhesion modules of the cell-bound biopolymer. The excretive production of the biopolymer PGB-1 coincided with the disruption of the cell-biopolymer network, most actively at the medium pH of 8.0.
Son
Mi-Kyung
MK
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
Hong
Soo-Jung
SJ
Sagong
Kuk-Hwa
KH
Lee
Yong-Hyun
YH
D001431:Bacteriological Techniques; D001704:Biopolymers / Q000096:biosynthesis*; D003470:Culture Media; D004754:Enterobacter / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure*; D005944:Glucosamine / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods; D019346:Sodium Acetate / Q000378:metabolism / Q000494:pharmacology*
D001704:Biopolymers; D003470:Culture Media; D019346:Sodium Acetate; D005944:Glucosamine