title: Comprehensive two-dimensional gas chromatography-mass spectrometry: a review.
abstract: Although comprehensive two-dimensional gas chromatography (GC x GC) has been on the scene for more than 15 years, it is still generally considered a relatively novel technique and is yet far from being fully established. The revolutionary aspect of GC x GC, with respect to classical multidimensional chromatography, is that the entire sample is subjected to two distinct analytical separations. The resulting enhanced separating capacity makes this approach a prime choice when GC analysts are challenged with highly complex mixtures. The combination of a third mass spectrometric dimension to a GC x GC system generates the most powerful analytical tool today for volatile and semi-volatile analytes. The present review is focused on the rather brief, but not scant, history of comprehensive two-dimensional GC-MS: the first experiments were carried out at the end of the 1990s and, since then, the methodology has been increasingly studied and applied. Almost all GC x GC-MS applications have been carried out by using either a time-of-flight or quadrupole mass analyzer; significant experiments relative to a variety of research fields, as well as advantages and disadvantages of the MS systems employed, are discussed. The principles, practical and theoretical aspects, and the most significant developments of GC x GC are also described.
authors.affiliation: Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università di Messina, viale Annunziata, 98168 Messina, Italy. lmondello@pharma.unime.it
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
authors.forename: Peter Quinto
authors.initials: PQ
authors.lastname: Tranchida
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Dugo
mesh_terms: D045424:Complex Mixtures / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans
chemical_list: D045424:Complex Mixtures