Resolving the database sequence discrepancies for the Staphylococcus aureus bacteriophage phi 11 amidase.
There are two conflicting primary nucleotide sequences of the Staphylococcus aureus bacteriophage phi 11 amidase gene in Genbank. Nucleotide sequence differences as well as alternative translational start site assignments result in three non-identical protein sequence predictions for this amidase. Therefore, it is prudent to verify the correct phi11 amidase protein sequence, especially since multiple versions of the amidase gene have been subcloned, deletion analysis performed, and their experimental use described. There is also a resurgence of interest in the expression and use of bacteriophage lytic proteins as bactericidal agents and the phi 11 amidase has a high antimicrobial potential. The correct amidase sequence is identified through a combination of DNA sequence analysis and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis of the recombinant purified phi11 amidase protein.
Donovan
David M
DM
Animal Biosciences and Biotechnology Laboratory, Animal and Natural Resources Institute, BARC, ARS, USDA, Baltimore Ave, Beltsville, MD, USA. ddonovan@anri.barc.usda.gov
Foster-Frey
Juli
J
Garrett
Wesley M
WM
Blomberg
LeAnn
L
D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics*; D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013204:Staphylococcus Phages / Q000201:enzymology* / Q000235:genetics*; D013211:Staphylococcus aureus / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; D000581:Amidohydrolases; C019665:amidase