Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay.
The various strategies available today to reassemble functional membrane proteins into model lipid bilayer systems are briefly described. Some of their virtues and weaknesses are discussed. The intimate relationships and complementarity between proteoliposomes and planar bilayers are illustrated.
Darszon
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Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14-740, 07000 México, DF.
D003902:Detergents; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D008823:Micelles; D008954:Models, Biological; D011510:Proteolipids / Q000737:chemistry / Q000378:metabolism
D003902:Detergents; D008051:Lipid Bilayers; D008081:Liposomes; D008565:Membrane Proteins; D008567:Membranes, Artificial; D008823:Micelles; D011510:Proteolipids; C020485:proteoliposomes