title: Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1-GTP.
abstract: The adaptor protein AP-1 is the major coat protein involved in the formation of clathrin-coated vesicles at the trans-Golgi network. The prevailing view is that AP-1 recruitment involves coincident binding to multiple low-affinity sites comprising adenosine diphosphate ribosylation factor 1 (Arf-1)-guanosine triphosphate (GTP), cargo sorting signals, and phosphoinositides. We now show that binding of cargo signal peptides to AP-1 induces a conformational change in its core domain that greatly enhances its interaction with Arf-1-GTP. In addition, we provide evidence for cross talk between the dileucine and tyrosine binding sites within the AP-1 core domain such that binding of a cargo signal to one site facilitates binding to the other site. The stable association of AP-1 with Arf-1-GTP, which is induced by cargo signals, would serve to provide sufficient time for adaptor polymerization and clathrin recruitment while ensuring the packaging of cargo molecules into the forming transport vesicles.
authors.affiliation: Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Intaek
authors.initials: I
authors.lastname: Lee
authors.forename: Balraj
authors.initials: B
authors.lastname: Doray
authors.forename: Jennifer
authors.initials: J
authors.lastname: Govero
authors.forename: Stuart
authors.initials: S
authors.lastname: Kornfeld
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002417:Cattle; D002966:Clathrin / Q000378:metabolism; D022163:Clathrin-Coated Vesicles / Q000378:metabolism / Q000648:ultrastructure; D006160:Guanosine Triphosphate / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D011108:Polymers / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D011487:Protein Conformation; D017434:Protein Structure, Tertiary / Q000235:genetics; D021381:Protein Transport / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D013347:Subcellular Fractions; D018808:Transcription Factor AP-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D022161:Transport Vesicles / Q000235:genetics*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D002966:Clathrin; D011108:Polymers; D018808:Transcription Factor AP-1; D014443:Tyrosine; D006160:Guanosine Triphosphate; D020823:ADP-Ribosylation Factor 1; D007930:Leucine