Fluorescence detection of glycoproteins in gels and on electroblots.
Glycosylation of proteins is a dynamic post-translational modification commonly found in eukaryotes, changing with development, differentiation, and disease state. Identification of glycoproteins is important for completely characterizing the population of proteins found at a given point in time. This unit contains protocols for identifying glycoproteins in general using a fluorescence based periodate/Schiff base method and those containing specific carbohydrates using a lectin-based fluorescent method.
Patton
Wayne F
WF
Molecular Probes, Inc., Eugene, Oregon, USA.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D005782:Gels / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D037102:Lectins / Q000737:chemistry; D008567:Membranes, Artificial; D010504:Periodic Acid / Q000737:chemistry; D012545:Schiff Bases / Q000737:chemistry
D005782:Gels; D006023:Glycoproteins; D037102:Lectins; D008567:Membranes, Artificial; D012545:Schiff Bases; D010504:Periodic Acid; C009288:metaperiodate