Comparative proteomics and difference gel electrophoresis.
The goal of comparative proteomics is to analyze proteome changes in response to development, disease, or environment. This is a two-step process in which proteins within cellular extracts are first fractionated to reduce sample complexity, and then the proteins are identified by mass spectrometry. Two-dimensional electrophoresis (2DE) is the long-time standard for protein separation, but it has suffered from poor reproducibility and limited sensitivity. Difference gel electrophoresis (DIGE), in which two protein samples are separately labeled with different fluorescent dyes and then co-electrophoresed on the same 2DE gel, was developed to overcome the reproducibility and sensitivity limitations. In this essay, I discuss the principles of comparative proteomics and the development of DIGE.
Minden
Jonathan
J
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
D004396:Coloring Agents; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods* / Q000639:trends; D020543:Proteome; D040901:Proteomics / Q000379:methods* / Q000639:trends; D012680:Sensitivity and Specificity
D004396:Coloring Agents; D020543:Proteome