[UV-light induced structural and functional changes of protein C4 of the complement system].
The influence of UV-light in the dose range of 75.5 - 2265 J/m2 on the structural and functional state of the C4 factor of the complement system has been studied by methods of hemolytic and acid-base titration, electrophoresis in polyacrylamide gel, infra-red and ultra-violet spectrophotometry. It was found that the UV-irradiation in doses of 75.5 and 755 J/m2 induces the photoactivation of the C4 protein due to changes in structures III and IV of the protein, which is accompanied by an increase in the number of aromatic amino acids and ionogenic groups on the protein molecule surface. The maximum dose of UV-irradiation (2265 J/m2) has a destructive effect on C4 protein, reducing its activity in the cascade of hemolytic reactions of the complement system. On the basis of the data obtained, a scheme of possible structural and functional photomodifications of C4 protein has been designed.
Artiukhov
V G
VG
Gusinskaia
V V
VV
Dvurekova
E A
EA
Rubtsov
M P
MP
D003181:Complement C4 / Q000737:chemistry / Q000528:radiation effects*; D006801:Humans; D011487:Protein Conformation; D014466:Ultraviolet Rays
D003181:Complement C4