Proteoglycan isolation and analysis.
Proteoglycans can be difficult molecules to isolate and analyze due to large mass, charge, and tendency to aggregate or form macromolecular complexes. This unit describes detailed methods for purification of matrix, cell surface, and cytoskeleton-linked proteoglycans. Methods for analysis of glycoaminoglycan size and type and of core protein species are described.
Woods
A
A
University of Alabama at Birmingham, Birmingham, Alabama, USA.
Couchman
J R
JR
D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification*
D006025:Glycosaminoglycans; D011509:Proteoglycans