title: Visualization of RNA using fluorescence complementation triggered by aptamer-protein interactions (RFAP) in live bacterial cells.
abstract: This unit describes a method allowing RNA visualization in live cells. The method is based on fluorescent protein complementation regulated by RNA-aptamer/RNA-binding protein interactions. Based on these two principles, a fluorescent ribonucleoprotein complex is assembled inside the cell only in response to the presence of the aptamer sequence on the target RNA.
authors.affiliation: Center for Advanced Biotechnology, College of Engineering, Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA.
authors.forename: Maria
authors.initials: M
authors.lastname: Valencia-Burton
authors.forename: Natalia E
authors.initials: NE
authors.lastname: Broude
mesh_terms: D020413:3' Untranslated Regions; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry*; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis*; D005470:Fluorometry / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding; D012329:RNA, Bacterial / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D012341:RNA, Ribosomal, 5S / Q000032:analysis
chemical_list: D020413:3' Untranslated Regions; D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012341:RNA, Ribosomal, 5S; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D039601:Eukaryotic Initiation Factor-4A