Using coupled monolithic rods for ultra-high peak capacity LC and LC-MS under normal LC operating pressures.
Up to ten individual reversed-phase monolithic LC columns were efficiently coupled to equal a total column length of 1 m. The column generated up to 90,000 theoretical plates under optimum conditions with a peak capacity under gradient conditions of 244 peaks h(-1) at a total column backpressure of just 140 bar, directly coupled to ESI-MS.
Bones
Jonathan
J
Centre for Bioanalytical Science, School of Chemical Sciences, Dublin City University, Dublin, Ireland.
Duffy
Cepta
C
Macka
Mirek
M
Paull
Brett
B
D000818:Animals; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D011506:Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
D011506:Proteins