The pheromone biosynthesis activating neuropeptide (PBAN) receptor of Heliothis virescens: identification, functional expression, and structure-activity relationships of ligand analogs.
Pheromone biosynthesis activating neuropeptide (PBAN) promotes synthesis and release of sex pheromones in moths. We have identified and functionally expressed a PBAN receptor from Heliothis virescens (HevPBANR) and elucidated structure-activity relationships of PBAN analogs. Screening of a larval CNS cDNA library revealed three putative receptor subtypes and nucleotide sequence comparisons suggest that they are produced through alternative splicing at the 3'-end. RT-PCR amplified preferentially HevPBANR-C from female pheromone glands. CHO cells expressing HevPBANR-C are highly sensitive to PBAN and related analogs, especially those sharing the C-terminal pentapeptide core, FXPRLamide (X=T, S or V). Alanine replacements in the C-terminal hexapeptide (YFTPRLamide) revealed the relative importance of each residue in the active core as follows: R5>L6>F2>>P4>T3>>Y1. This study provides a framework for the rational design of PBANR-specific agonists and/or antagonists that could be exploited for disruption of reproductive function in agriculturally important insect pests.
Kim
Young-Joon
YJ
Department of Entomology, 2103 Biological Sciences Building, University of California, Riverside, CA 92521, United States.
Nachman
Ronald J
RJ
Aimanova
Karlygash
K
Gill
Sarjeet
S
Adams
Michael E
ME
D000331:Aequorin / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D020013:Calcium Signaling / Q000187:drug effects; D002490:Central Nervous System / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics; D007301:Insect Hormones / Q000494:pharmacology; D019476:Insect Proteins / Q000819:agonists / Q000235:genetics / Q000502:physiology*; D007915:Lepidoptera / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism* / Q000494:pharmacology; D010802:Phylogeny; D020033:Protein Isoforms / Q000819:agonists / Q000235:genetics / Q000502:physiology; D018013:Receptors, Neuropeptide / Q000819:agonists / Q000235:genetics / Q000502:physiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D007301:Insect Hormones; D019476:Insect Proteins; D009479:Neuropeptides; D020033:Protein Isoforms; D018013:Receptors, Neuropeptide; C403307:ecdysis-triggering hormone, Drosophila; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea; D000331:Aequorin; C075027:diapause hormone; C112631:G protein alpha 16; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins