Purification and analysis of thrombospondin-1.
Thromboapondin 1 (TSP-1) is a trimeric matricellular protein that is expressed by many cells. It contains several different domains that allow it to participate in cell adhesion, cell migration, and cell signaling. Recently TSP-1 has been shown to activate transforming growth factor beta (TGF-beta) and to inhibit both angiogenesis and tumor growth. This unit contains protocols for the purification of TSP-1 from platelet-rich plasma and the purification of TSP-1 proteolytic fragments.
Yee
Karen O
KO
Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
Duquette
Mark
M
Ludlow
Anna
A
Lawler
Jack
J
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000737:chemistry*; D006801:Humans; D008722:Methods; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000737:chemistry; D019700:Thrombospondin 1 / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
D010446:Peptide Fragments; D019700:Thrombospondin 1; D010447:Peptide Hydrolases