A viable Bacillus subtilis strain without functional extracytoplasmic function sigma genes.
We constructed a Bacillus subtilis Marburg strain that harbors deletion mutations in all seven extracytoplasmic function (ECF) sigma genes. The strain shows wild-type growth at 37 degrees C both in a complex and in a synthetic medium and exhibits wild-type sporulation. ECF sigma genes of B. subtilis are dispensable as long as no stress is imposed, although they seem to be required for quick response to stresses.
Asai
Kei
K
Department of Molecular Biology, Faculty of Science, Saitama University, Saitama 338-8570, Japan.
Ishiwata
Keisuke
K
Matsuzaki
Kunihiko
K
Sadaie
Yoshito
Y
D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D050296:Microbial Viability / Q000235:genetics*; D008954:Models, Biological; D009154:Mutation; D016133:Polymerase Chain Reaction; D012808:Sigma Factor / Q000235:genetics* / Q000502:physiology; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology
D001426:Bacterial Proteins; D012808:Sigma Factor