Preparation and use of interphase Xenopus egg extracts.
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.
Powers
M
M
Emory University School of Medicine, Atlanta, Georgia, USA.
Evans
E K
EK
Yang
J
J
Kornbluth
S
S
D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D002457:Cell Extracts / Q000302:isolation & purification*; D003584:Cytological Techniques; D004261:DNA Replication / Q000502:physiology; D005260:Female; D007399:Interphase / Q000502:physiology*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D014981:Xenopus
D002457:Cell Extracts