Human embryonic stem cell derivation from poor-quality embryos.
During in vitro fertilization, embryos deemed clinically useless based on poor morphology are typically discarded. Here we demonstrate a statistical correlation between the developmental stage of such poor-quality embryos and the yield of human embryonic stem (hES) cell lines. Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells.
Lerou
Paul H
PH
Division of Newborn Medicine, Brigham & Women's Hospital, Boston, Massachusetts 02115, USA.
Yabuuchi
Akiko
A
Huo
Hongguang
H
Takeuchi
Ayumu
A
Shea
Jessica
J
Cimini
Tina
T
Ince
Tan A
TA
Ginsburg
Elizabeth
E
Racowsky
Catherine
C
Daley
George Q
GQ
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans