[Detection of Y chromosome microdeletions in AZF region by liquid chip technology].
OBJECTIVE
To establish a liquid chip technology to detect Y chromosome microdeletions in Chinese infertile males with azoospermia or oligozoospermia.


METHODS
Multiplex PCR and liquid chip technology were used to detect the Y chromosome microdeletions in AZF region in 178 infertile patients with azoospermia and 134 infertile patients with oligozoospermia as well as 40 fertile control men.


RESULTS
Forty out of 312 patients (12.8%) were found to have deletions in AZF region. The microdeletion frequency was 14%(25/178) in the azoospermic group, 9.6%(11/114) in the oligospermic and 20%(4/20) in the severe oligospermic group.


CONCLUSIONS
The authors developed a high-throughput, fast and simple assay to screen the AZF region microdeletions of Y chromosome.
Liu
Si-yao
SY
Sichuan University, Sichuan, 610064 People's Republic of China.
Ding
Xian-ping
XP
Wei
Xia
X
Wei
Ping
P
Pan
Hai-rong
HR
D044466:Asian People / Q000235:genetics; D053713:Azoospermia / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D009845:Oligospermia / Q000235:genetics*; D016324:Sequence Tagged Sites