title: Dictyostelium cell dynamics.
abstract: This unit describes culturing and imaging of D. discoideum amoebae to study fundamental cellular responses, such as motility and directed migration. The system displays powerful molecular genetics that can be used to link structural determinants of proteins with in vivo cellular functions.
authors.affiliation: National Cancer Institute, NIH, Bethesda, Maryland, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Parent
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D054443:Cell Migration Assays / Q000379:methods* / Q000592:standards; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D002630:Chemotactic Factors / Q000737:chemistry / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects / Q000235:genetics; D003470:Culture Media / Q000138:chemical synthesis / Q000494:pharmacology; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000138:chemical synthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods
chemical_list: D002630:Chemotactic Factors; D003470:Culture Media; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins