Induction of differentiation of myeloid leukemia cells in primary culture in response to lithocholic acid acetate, a bile acid derivative, and cooperative effects with another differentiation inducer, cotylenin A.
Lithocholic acid (LCA) acetate induced the differentiation of human leukemia cells. Treatment with a combination of LCA acetate and cotylenin A, an inducer of the differentiation of leukemia cells, was more effective than that with LCA acetate or cotylenin A alone at inducing monocytic differentiation. LCA acetate activated mitogen-activated protein kinase (MAPK) before inducing differentiation. Cotylenin A did not activate MAPK, suggesting that cotylenin A has a different mode of action. The cooperative effects of LCA acetate and cotylenin A on inducing differentiation were, at least partly, due to the enhancement of LCA acetate-induced MAPK activation by cotylenin A.
Horie
Akiyoshi
A
Department of Life Science, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
Akimoto
Miho
M
Tsumura
Hiroto
H
Makishima
Makoto
M
Taketani
Takeshi
T
Yamaguchi
Seiji
S
Honma
Yoshio
Y
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D017931:DNA Primers; D004224:Diterpenes / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D004224:Diterpenes; C423222:cotylenin A; C502354:lithocholic acid acetate; D008095:Lithocholic Acid; D020928:Mitogen-Activated Protein Kinases