Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus.
A cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection of cdtA, cdtB or cdtC gene of Campylobacter jejuni, Campylobacter coli or Campylobacter fetus, respectively, was developed and evaluated with 76 Campylobacter strains belonging to seven different species and 131 other bacterial strains of eight different genera. The cdtA, cdtB or cdtC gene of C. jejuni, C. coli or C. fetus, respectively, could be successfully amplified using the corresponding set of primers in a highly species-specific manner. Furthermore, the specific primer set for the cdtA, cdtB or cdtC gene of a particular species could amplify the desired gene from a mixture of DNA templates of any of two or all three species. The detection limit of C. jejuni, C. coli or C. fetus was 10-100 CFU tube(-1) by the multiplex PCR assay on the basis of the presence of the cdtA, cdtB or cdtC gene. These data indicate that the cdt gene-based multiplex PCR assay may be useful for rapid and accurate detection as well as identification of Campylobacter strains in a species-specific manner.
Asakura
Masahiro
M
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Samosornsuk
Worada
W
Hinenoya
Atsushi
A
Misawa
Naoaki
N
Nishimura
Kazuhiko
K
Matsuhisa
Akio
A
Yamasaki
Shinji
S
D001427:Bacterial Toxins / Q000235:genetics*; D002169:Campylobacter Infections / Q000382:microbiology; D017000:Campylobacter coli / Q000235:genetics / Q000302:isolation & purification*; D002168:Campylobacter fetus / Q000235:genetics / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins; D004269:DNA, Bacterial; C067044:cytolethal distending toxin