Changes in the activity of the multifunctional beta-glycosyl hydrolase (Cel44C-Man26A) from Paenibacillus polymyxa by removal of the C-terminal region to minimum size.
Paenibacillus polymyxa GS01 secretes Cel44C-Man26A as a multifunctional enzyme with cellulase, xylanase, lichenase, and mannanase activities. Cel44C-Man26A consists of 1,352 amino acids in which present a catalytic domain (CD) of the glycosyl hydrolase family 44 (GH44), fibronectin domain type 3 (Fn3), catalytic domain of glycosyl hydrolase family 26 (GH26), and a cellulose-binding module type 3 (CBM3). A truncated Cel44C-Man26A protein, consisting of 549 amino acid residues, reacted as a multifunctional mature enzyme despite the absence of the 10 amino acids containing GH44, Fn3, GH26, and CBM3. However, the multifunctional activity was not found in the mature Cel44C-Man26A protein truncated to less than 548 amino acids. The truncated Cel44C-Man26A proteins showed the optimum pH for the lichenase activity was pH 7.0, pH 6.0 for the xylanase and mannanase, and pH 5.0 for the cellulase. The truncated Cel44C-Man26A proteins exhibited enzymatic activity 40-120% higher than the full-length Cel44C.
Cho
Kye-Man
KM
Division of Applied Life Science, Gyeongsang National University, Chinju, 660-701, Korea.
Math
Renukaradhya K
RK
Hong
Su-Young
SY
Asraful Islam
Shah Md
SM
Kim
Jong-Ok
JO
Hong
Sun-Joo
SJ
Kim
Hoon
H
Yun
Han-Dae
HD
D000595:Amino Acid Sequence; D019677:Artificial Gene Fusion; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D044602:Cellulases / Q000378:metabolism; D003001:Cloning, Molecular; D018389:Codon, Nonsense / Q000235:genetics; D004274:DNA, Recombinant / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D044902:beta-Mannosidase / Q000378:metabolism
D001426:Bacterial Proteins; D018389:Codon, Nonsense; D004274:DNA, Recombinant; D044602:Cellulases; D006026:Glycoside Hydrolases; D044902:beta-Mannosidase; C028020:licheninase; D043364:Endo-1,4-beta Xylanases