Metabolic labeling of glycoproteins with radioactive sugars.
This unit describes methods for preparation of glycoproteins metabolically labeled with radioactive sugars, sulfate, and phosphate. Methods for liberation of both N- and O-linked glycans are also described. These protocols can be used to generate materials for characterization of glycoprotein glycans from cultured cells.
Snider
Martin D
MD
Case Western Reserve University, Cleveland, Ohio, USA.
D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D019411:Clinical Laboratory Techniques; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008660:Metabolism; D011868:Radioisotopes; D012107:Research Design
D002241:Carbohydrates; D006023:Glycoproteins; D011868:Radioisotopes