[Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene].
OBJECTIVE
To construct the virus-like parcel expressing hepatitis B virus (HBV) C gene and identify its immunogenicity.


METHODS
HBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara (MVA).


RESULTS
pSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immunogenicity.


CONCLUSIONS
The MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
Luan
Xiang-ling
XL
Postgraduate Medical School of PLA, Beijing 100853, China. luanxiangling@sohu.com
Kong
Wei
W
Liu
Su-jun
SJ
Lei
Li
L
Hu
Yan
Y
Hou
Jun
J
Shen
Hong-hui
HH
Wu
Yi-chen
YC
You
Shao-li
SL
Mao
Pan-yong
PY
Xin
Shao-jie
SJ
D005814:Genes, Viral; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics*
D006512:Hepatitis B Core Antigens