Characterization of monoclonal antibodies to an antigenic protein from Stachybotrys chartarum and its measurement in house dust.
Using sera from atopic patients we have isolated an extracellular protein, which is antigenic in humans, from Stachybotrys chartarum sesu lato. Here we report the production of monoclonal antibodies to the protein and the development of a sensitive and specific assay to the target protein as well as analyses in house dust samples spiked with spores. The detection limit for the target antigen in house dust was approximately 0.2 ng/g dry weight house dust. This detection limit is comparable to those for house dust mite allergen and the allergen of the fungus Aspergillus fumigatus but lower than that for the fungus Alternaria alternata.
Xu
Jianping
J
Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, Canada ON K1S 5B6.
Liang
Yinan
Y
Belisle
Donald
D
Miller
J David
JD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D000946:Antigens, Fungal / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D004391:Dust / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D015203:Reproducibility of Results; D013172:Spores, Fungal / Q000737:chemistry / Q000276:immunology; D013191:Stachybotrys / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D004391:Dust; D005656:Fungal Proteins