Inhibition of vascular endothelial growth factor expression in keloid fibroblasts by vector-mediated vascular endothelial growth factor shRNA: a therapeutic potential strategy for keloid.
Vascular endothelial growth factor (VEGF) plays important roles in the regulation of angiogenesis and inflammation in both pathological and physiological wound repair. Several strategies have been developed for keloid therapy; however, a universally effective treatment has not been explored to date. In this study, three potential short interfering RNA (siRNA) sequences for the VEGF gene were cloned into expression plasmids and transfected into keloid fibroblasts. PGC-VEGF shRNA 2 (short hairpin RNA 2) plasmid significantly inhibited VEGF expression determined by real-time polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and fibroblasts growth was also significantly by (methyl thiazolyl tetrazolium) MTT assay and apoptosis detection, whereas the control transfection showed no obviously effects. Plasminogen activator inhibitor-1 (PAI-1) expression in pGC-VEGF shRNA2 group was also obviously downregulated when compared to the pGC-VEGF shRNA negative control and mock group. These results suggest that modulation of VEGF production by vector-based RNAi in keloid fibroblasts may be a therapeutic potential strategy for keloid.
Zhang
Guo-You
GY
Department of Burn and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, 530021, Nanning, China.
Yi
Cheng-Gang
CG
Li
Xuan
X
Zheng
Yan
Y
Niu
Zhan-Guo
ZG
Xia
Wei
W
Meng
Zhou
Z
Meng
Cheng-Yue
CY
Guo
Shu-Zhong
SZ
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D007627:Keloid / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D008297:Male; D009389:Neovascularization, Pathologic / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
D017395:Plasminogen Activator Inhibitor 1; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A