title: Levels of transaminases, alkaline phosphatase, and protein in tissues of Clarias gariepienus fingerlings exposed to sublethal concentrations of cadmium chloride.
abstract: The freshwater fish, Clarias gariepienus fingerlings, were exposed to sublethal concentrations (1.7 and 3.4 mg/L) of cadmium chloride for 12 days. Aspartate aminotransferase (AAT), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total protein levels were assayed in the gill, brain, and muscle of the fish at regular intervals of 6 and 12 days. The activities of AAT, ALT, and ALP of the treated fishes increased significantly in all the tissues compared with the control fish. Protein level in all the tissues showed a significant decrease in comparison to unexposed controls throughout the experimental periods. These results revealed that cadmium chloride effects the intermediary metabolism of C. gariepienus fingerlings and that the assayed enzymes can work as good biomarkers of contamination.
authors.affiliation: Environmental Science and Biotechnology Research Unit, Department of Zoology, Loyola College, Chennai 600 034, Tamil Nadu, India.
authors.forename: Babu
authors.initials: B
authors.lastname: Velmurugan
authors.forename: Mariadoss
authors.initials: M
authors.lastname: Selvanayagam
authors.forename: Elif I
authors.initials: EI
authors.lastname: Cengiz
authors.forename: Ersin
authors.initials: E
authors.lastname: Uysal
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D019256:Cadmium Chloride / Q000493:pharmacokinetics / Q000633:toxicity*; D005399:Fishes; D005618:Fresh Water; D005880:Gills / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007306:Insecticides / Q000493:pharmacokinetics / Q000633:toxicity; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D000637:Transaminases / Q000032:analysis / Q000378:metabolism*
chemical_list: D007306:Insecticides; D011506:Proteins; D000637:Transaminases; D000469:Alkaline Phosphatase; D019256:Cadmium Chloride