Anti-apoptotic seminal vesicle protein IV inhibits cell-mediated immunity.
The in vitro effect of seminal vesicle protein IV (SV-IV) on the cytotoxic activity of human natural or acquired cellular immunity has been investigated by standard immunological procedures, a (51)Cr-release cytotoxicity assay, and labeled-ligand binding experiments. The data obtained demonstrate that: (1) fluoresceinated or [(125)I]-labeled SV-IV binds specifically to the surface of human purified non-adherent mononuclear cells (NA-MNC); (2) SV-IV suppresses the cytotoxicity of natural killer (NK) cells against K562 target cells, that of IL-2-stimulated NK (LAK) cells against DAUDI target cells, and that of VEL antigen-sensitized cytotoxic T lymphocytes (CTLs) against VEL target cells; (3) treatment of K562 target cells alone with SV-IV decreases their susceptibility to NK-induced lysis. These findings indicate that the protein SV-IV has a marked in vitro inhibitory effect on NK, LAK and CTL cytotoxicity, providing a better understanding of its immune regulatory functions.
Fuggetta
M P
MP
Institute of Neurobiology and Molecular Medicine (INMM), CNR, Area Ricerca Roma Tor Vergata, Via del Fosso del Cavaliere 100, Rome, Italy.
Lanzilli
G
G
Cottarelli
A
A
Ravagnan
G
G
Cartenì
M
M
De Maria
S
S
Metafora
B M
BM
Metafora
V
V
Metafora
S
S
D045744:Cell Line, Tumor; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D020014:K562 Cells; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology* / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D029607:Seminal Vesicle Secretory Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
D029607:Seminal Vesicle Secretory Proteins