title: Studies of diarrhetic activity on pectenotoxin-6 in the mouse and rat.
abstract: Diarrhetic activity of pectenotoxin-6 (PTX6), a shellfish contaminant in Japanese scallops (Patinopecten yessoensis), was studied in vivo. Mice gavaged with 5mg/kg PTX6 did not show diarrhea or fluid secretion, and no prominent pathological changes were observed. There was no synergistic toxicity of PTX6 with okadaic acid (OA) or pectenotoxin-2 (PTX2) when toxins were given to mice by gavage. Synergistic activity of PTX6 with OA was also not confirmed under crude conditional simulation with oil. In contrast to the oral administration to mice, PTX6 at 500 microg/kg by i.p. was the lethal dose with bleeding in the liver, injuries at the gastric organs and the kidney. When rats were gavaged with PTX6 at a dose of 2 mg/kg, PTX6 did not have diarrhetic activity; however, the middle-lower small intestine (jejunum-ileum) was eroded at villi by edema. PTX6 is a potent toxin if administered by intraperitoneal injection to mice, or if administered orally to the rat. However, it is not clear if PTX6 passes through the intestinal barrier if given by the oral route.
authors.affiliation: Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Ito
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yasukatsu
authors.initials: Y
authors.lastname: Oshima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasumoto
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000009:adverse effects* / Q000737:chemistry / Q000378:metabolism; D007421:Intestine, Small / Q000473:pathology / Q000648:ultrastructure; D018942:Macrolides; D008297:Male; D008387:Marine Toxins / Q000009:adverse effects* / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D019319:Okadaic Acid / Q000494:pharmacology; D049897:Pectinidae / Q000378:metabolism; D011714:Pyrans / Q000009:adverse effects* / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D000095702:Polyether Toxins
chemical_list: D005663:Furans; D018942:Macrolides; D008387:Marine Toxins; D019319:Okadaic Acid; D011714:Pyrans; C114399:pectenotoxin 6; D000095702:Polyether Toxins; C098380:pectenotoxin 2