[Toluene diisocyanate increases vascular endothelial growth factor expression in human bronchial epithelial cells].
OBJECTIVE
To investigate the effect of toluene diisocyanate (TDI) on the expression of vascular endothelial growth factor (VEGF) in human bronchial epithelial (HBE) cells.


METHODS
TDI-human serum albumin (TDI- HSA) conjugate was prepared using a modified Son's method. MTT assay was used to examine the viability of HBE135-E6E7 cells cultured in serum-free medium after treatment with HSA or TDI-HSA at different concentrations. VEGF mRNA expression of the HBE cells treated with HSA or TDI-HSA at 10, 20, 30 and 40 microg/ ml, respectively, was detected using semi-quantitative RT-PCR.


RESULTS
Treatment with 40 microg/ml HSA and 40 microg/ml TDI-HSA did not result in significant changes in the viability of HBE135-E6E7 cells. RT-PCR revealed the constitutive expression of two VEGF isoforms, namely VEGF189 and VEGF165, in cultured HBE135-E6E7 cells. After exposure to TDI-HSA at the different concentrations (except for 10 microg/ml), a significant increase occurred in both VEGF189 and VEGF165 mRNA expressions in HBE135-E6E7 cells as compared with the expressions in the control group and the HSA-treated cells (P<0.05), and significant dose dependence was noted in the effect of TDI-HSA (P<0.05). No significant difference was found in the expressions between the control cells and the HAS-treated cells (P>0.05).


CONCLUSIONS
TDI induces significant increase in VEGF expression in HBE cells, and VEGF overexpression may play an important role in the pathogenesis of TDI-induced asthma.
Peng
Hong-juan
HJ
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lisiyiabc@tom.com
Cai
Shao-xi
SX
Zhao
Hai-jin
HJ
Li
Wen-jun
WJ
Tong
Wan-cheng
WC
D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D002470:Cell Survival; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D020033:Protein Isoforms; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D014051:Toluene 2,4-Diisocyanate