Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.
A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.
Nozawa-Inoue
Mamie
M
Department of Land, Air, and Water Resources, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. minoue@ucdavis.edu
Jien
Mercy
M
Hamilton
Nicholas S
NS
Stewart
Valley
V
Scow
Kate M
KM
Hristova
Krassimira R
KR
D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010472:Perchlorates / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010472:Perchlorates; D010088:Oxidoreductases; C494474:perchlorate