[Experimental study of portal hemodynamics after microcapsule transplantation by intraportal and transarterial approach].
OBJECTIVE
To compare the changes of portal hemodynamics after microcapsule transplantation by intraportal and transarterial approach with color doppler sonography.


METHODS
Thirty male dogs were divided into 2 groups randomly: Group PV (microcapsules implanted into the portal vein) and Group HA (microcapsules implanted into the hepatic artery). Both groups were divided into 3 subgroups(PV1, PV2, and PV3; HA1, HA2, and HA3) according to the number of implanted microcapsules (8,000/kg, 16,000/kg, and 32,000/kg). The internal diameter and flow velocity of the portal vein were measured with color doppler sonography before the transplantation,within 24 hours and 1, 2, and 4 weeks after microcapsule transplantation.


RESULTS
The flow velocity of the portal vein in Group PV1 and PV2 within 24 hours increased significantly (P<0.05); The meanwhile the internal diameters did not change significantly (P> 0.05). The flow velocity of the portal vein in Group PV3 within 24 hours decreased significantly (P<0.05), but the internal diameter increased significantly (P<0.05). Portal hemodynamics of different transarterial subgroups did not change significantly (P> 0.05). The level of ALT in Group PV and Group HA all increased significantly (P<0.05). And when the numbers of microcapsule transplantation were same, the level of ALT in subgroup PV were significantly higher than that in subgroup HA (P<0.05).


CONCLUSIONS
Microcapsule transplantation by transarterial approach is safer than by intraportal way, and the hepatic artery can contain more microcapsule than the portal vein.
Gao
Feng
F
Center of Cell Transplantation and Gene Therapy, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Wang
Wei
W
Li
Rui-zhen
RZ
Liu
Sheng
S
Zhou
Ping
P
Zheng
Wei
W
Ai
Shao-dong
SD
D000464:Alginates; D000818:Animals; D002214:Capsules; D004285:Dogs; D020723:Glucuronic Acid; D006439:Hemodynamics; D006499:Hepatic Artery; D006603:Hexuronic Acids; D007261:Infusions, Intra-Arterial / Q000379:methods*; D007262:Infusions, Intravenous / Q000379:methods; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D008297:Male; D011169:Portal Vein / Q000000981:diagnostic imaging / Q000503:physiopathology*; D011897:Random Allocation; D018615:Ultrasonography, Doppler, Color
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid