title: Molecular characterization of group A human rotaviruses in Bangkok and Buriram, Thailand during 2004-2006 reveals the predominance of G1P[8], G9P[8] and a rare G3P[19] strain.
abstract: The aim of this study has been to determine the incidence of diverse human rotavirus strains circulating in Thailand between October 2004 and April 2006 by means of molecular characterization. Pediatric patients aged between 2 months and 5 years diagnosed with acute diarrhea (n=307) in Bangkok and Buriram, Thailand were tested for human rotavirus A (RV-A) by RT-PCR. A total of 130 specimens (42.3%) were found RV-A positive and 126 were characterized by direct sequencing of the capsid glycoproteins VP7 and VP4. BLAST/FASTA analysis and phylogenetic analysis revealed genotypes G1P[8] (85.7%), G2P[4] (2.4%), G2P[8] (0.8%), G3P[8] (1.6%), G9P[8] (8.7%), and the uncommon strain G3P[19] (0.8%). Varying sites of polymorphism over time imply dependence on geographical location along with seasonal variation of relative incidence and distribution of rotavirus types. Thus, continuous molecular monitoring of human rotavirus epidemiology is essential for adjusting vaccine development.
authors.affiliation: Faculty of Medicine, Department of Pediatrics, Viral Hepatitis Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand.
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Parvapan
authors.initials: P
authors.lastname: Bhattarakosol
authors.forename: Voranush
authors.initials: V
authors.lastname: Chongsrisawat
authors.forename: Thrissawan
authors.initials: T
authors.lastname: Sungkapalee
authors.forename: Norra
authors.initials: N
authors.lastname: Wutthirattanakowit
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology; D012621:Seasons; D016415:Sequence Alignment; D013785:Thailand / Q000453:epidemiology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus