Influence of the stationary phase on the stability of thalidomide and comparison of different methods for the quantification of thalidomide in tablets using high-temperature liquid chromatography.
In this paper, three different HPLC methods for the quantification of thalidomide in tablets were developed and compared. The comparison of a conventional method at 30 degrees C with two high-temperature methods at 180 degrees C showed equal results. Using high-temperature HPLC (HT-HPLC), faster analysis times could be achieved. We have also focused on analyte stability and could show that the stationary phase has a pronounced effect on the on-column degradation of thalidomide at high temperatures. Virtually no degradation occurs if a polystyrene divinylbenzene column is used, whereas thalidomide is completely degraded at 180 degrees C when a carbon clad zirconium dioxide column is used.
Giegold
Sascha
S
Institute of Energy and Environmental Technology e. V. (IUTA), Bliersheimer Str. 60, D-47229 Duisburg, Germany.
Holzhauser
Maren
M
Kiffmeyer
Thekla
T
Tuerk
Jochen
J
Teutenberg
Thorsten
T
Rosenhagen
Maike
M
Hennies
Dorothea
D
Hoppe-Tichy
Torsten
T
Wenclawiak
Bernd
B
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D013607:Tablets; D013792:Thalidomide / Q000032:analysis* / Q000737:chemistry*
D013607:Tablets; D013792:Thalidomide