title: Preparation of human epidermal keratinocyte cultures.
abstract: Cultured keratinocytes have been used by a number of investigators in studies investigating wound repair and carcinogenesis, and they have also proven useful as a model for differentiation. This unit describes a protocol for establishing human keratinocytes in tissue culture. Human newborn foreskins are proteolytically digested to separate the epidermis and the dermis, and keratinocytes are obtained from the epidermis.
authors.affiliation: National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Susan S
authors.initials: SS
authors.lastname: Yamada
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D003470:Culture Media; D000078404:Epidermal Cells; D052816:Foreskin / Q000166:cytology; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000166:cytology*; D008297:Male; D014357:Trypsin
chemical_list: D003470:Culture Media; D014357:Trypsin