Immunostaining for p16INK4a used as a conjunctive tool improves interobserver agreement of the histologic diagnosis of cervical intraepithelial neoplasia.
The quality of cervical histopathology is critical to cervical cancer prevention, cancer treatment, and research programs. On the basis of the histology results further patient management is determined. However, the diagnostic interpretation of histologic hematoxylin-eosin (H&E)-stained slides is affected by substantial rates of discordance among pathologists. Overexpression of the cyclin-dependent kinase inhibitor p16INK4a, a cell cycle regulating protein, has been shown to be strongly correlated with dysplastic lesions of the cervix uteri. In this study, we assessed whether p16INK4a immunohistochemistry may increase the performance of pathologists in diagnosing squamous lesions in cervical punch and cone biopsies. When using a consecutive p16INK4a-stained slide in conjunction to the H&E-stained slide, interobserver agreement between 6 pathologists improved significantly for both cervical punch and cone biopsies (P < 0.001). For punch biopsies (n = 247), kappa value increased from 0.49 (moderate agreement) to 0.64 indicating substantial agreement, and interobserver agreement for cone biopsies (n = 249) improved from 0.63 (conventional H&E slide reading) to 0.70 when H&E-stained slides were read conjunctively with p16INK4a-stained slides. In comparison to a common consensus diagnosis established by 3 independent experts, 4 pathologists reached an improvement with the conjunctive p16INK4a test, 2 of them showing significantly better agreement (P < 0.001 and P = 0.002, respectively), p16INK4a immunohistochemistry as an adjunct to conventional H&E-stained specimens thus contributes to a more reproducible diagnosis of cervical intraepithelial neoplasia and may be a valuable aid for the interpretation of cervical histology.
Horn
Lars-Christian
LC
Institute of Pathology, University of Leipzig, Germany.
Reichert
Anja
A
Oster
Anne
A
Arndal
Sanne Frost
SF
Trunk
Marcus J
MJ
Ridder
Ruediger
R
Rassmussen
Ole Feldballe
OF
Bjelkenkrantz
Kaj
K
Christiansen
Pernille
P
Eck
Matthias
M
Lorey
Thomas
T
Skovlund
Vibeke Ravn
VR
Ruediger
Thomas
T
Schneider
Volker
V
Schmidt
Dietmar
D
D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D004396:Coloring Agents; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D004801:Eosine Yellowish-(YS); D005260:Female; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry; D015588:Observer Variation; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000737:chemistry / Q000175:diagnosis* / Q000473:pathology
D014408:Biomarkers, Tumor; D004396:Coloring Agents; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin