Regulation of stretch-activated ANP secretion by chloride channels.
This study was aimed to define roles of stretch-activated ion channels (SACs), especially Cl(-) channels, in regulation of atrial natriuretic peptide (ANP) secretion using isolated perfused beating atria. The volume load was achieved by elevating height of outflow catheter connected to isolated rat atria and the pressure load was achieved by decreasing diameter of outflow catheter. Both methods increased atrial contractility similarly although volume load was different (736microl for volume load vs. 129microl for pressure load). Atrial stretch by volume load markedly increased ECF translocation and ANP secretion but the pressure load slightly increased. The ANP secretion was positively correlated to workload generated by volume or pressure load. Treatment of atria with gadolinium, a blocker for SACs, attenuated the ECF translocation and the ANP secretion induced by volume load. A blocker for Ca2+-activated Cl(-) channel, niflumic acid (NFA), accentuated the ANP secretion induced by volume load whereas a blocker for swelling-activated Cl(-) channel, diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS), attenuated the ANP secretion. The ANP secretion of hypertrophied atria by volume load was markedly reduced and the augmented effect of NFA on volume load-induced ANP secretion was not observed. These results indicate that Cl(-) channels may differently regulate stretch-activated ANP secretion.
Han
Jeong Hee
JH
2-20 Keum-Am-Dong-San, Department of Physiology, Institute for Medical Sciences, Chonbuk National University Medical School, Jeonju 561-180, Republic of Korea.
Bai
Guang Yi
GY
Park
Jae-Hyeong
JH
Yuan
Kuichang
K
Park
Woo Hyun
WH
Kim
Sung Zoo
SZ
Kim
Suhn Hee
SH
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005682:Gadolinium / Q000494:pharmacology; D006325:Heart Atria / Q000378:metabolism; D008297:Male; D009200:Myocardial Contraction; D009544:Niflumic Acid / Q000494:pharmacology; D051381:Rats
D018118:Chloride Channels; D009544:Niflumic Acid; D009320:Atrial Natriuretic Factor; D005682:Gadolinium