title: Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts.
abstract: Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L(6)E(9) myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors.forename: Boumediene
authors.initials: B
authors.lastname: Bouzahzah
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Yurchenko
authors.forename: Fnu
authors.initials: F
authors.lastname: Nagajyothi
authors.forename: James
authors.initials: J
authors.lastname: Hulit
authors.forename: Moshe
authors.initials: M
authors.lastname: Sadofsky
authors.forename: Vicki L
authors.initials: VL
authors.lastname: Braunstein
authors.forename: Shankar
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Hannah
authors.initials: H
authors.lastname: Weiss
authors.forename: Fabiana S
authors.initials: FS
authors.lastname: Machado
authors.forename: Richard G
authors.initials: RG
authors.lastname: Pestell
authors.forename: Michael P
authors.initials: MP
authors.lastname: Lisanti
authors.forename: Herbert B
authors.initials: HB
authors.lastname: Tanowitz
authors.forename: Chris
authors.initials: C
authors.lastname: Albanese
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000378:metabolism*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008156:Luciferases; D032446:Myoblasts / Q000378:metabolism* / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D019938:Cyclin D1; D008156:Luciferases