Fluorescent staining of subcellular organelles: ER, Golgi complex, and mitochondria.
The ability to distinguish and identify specific subcellular compartments is essential to understanding organelle function, biogenesis, and maintenance within cells and to defining protein trafficking pathways. Fluorescent dyes and/or fluorescently labeled lipid derivatives can be used to identify ER, Golgi complex, and mitochondria. Specific conditions for labeling each of these compartments are described.
Terasaki
M
M
University of Connecticut Health Center, Farmington, Connecticut, USA.
Loew
L
L
Lippincott-Schwartz
J
J
Zaal
K
K
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D000818:Animals; D002232:Carbocyanines / Q000032:analysis / Q000737:chemistry; D002478:Cells, Cultured / Q000648:ultrastructure; D002518:Ceramides / Q000032:analysis / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D008928:Mitochondria / Q000737:chemistry / Q000648:ultrastructure*; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods; D013110:Sphingosine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
D002232:Carbocyanines; D002518:Ceramides; D004396:Coloring Agents; D005456:Fluorescent Dyes; C527864:N-(5-(5,7-dimethyl Bodipy)-1-pentanoyl)-erythrosphingosine; D012235:Rhodamines; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; C005358:tetramethylrhodamine; C037974:N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl sphingosine; D009327:4-Chloro-7-nitrobenzofurazan; D013110:Sphingosine