Hapten synthesis and monoclonal antibody-based immunoassay development for the detection of the fungicide kresoxim-methyl.
Strobilurin fungicides have been increasingly used for fungus pest control since they were introduced in 1996. For pesticide residue detection, immunoassays constitute nowadays a valuable approach. This paper describes the synthesis of functionalized haptens of kresoxim-methyl, the production of monoclonal antibodies, and the development of enzyme-linked immunosorbent assays. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.4 ng/mL for the extended assay and 0.3 ng/mL for the rapid assay. On the other hand, an immunoassay was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.4 ng/mL. All of these assays showed a similar performance, with sensitivities well below common maximum residue limits for this pesticide (50 microg/kg) and lower than the detection limits of the usual chromatographic detection methods.
Mercader
Josep V
JV
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, 46100 Burjassot, València, Spain.
Suárez-Pantaleón
Celia
C
Agulló
Consuelo
C
Abad-Somovilla
Antonio
A
Abad-Fuentes
Antonio
A
D000911:Antibodies, Monoclonal; D003257:Consumer Product Safety; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005659:Fungicides, Industrial / Q000032:analysis*; D006241:Haptens / Q000276:immunology; D006801:Humans; D008689:Methacrylates / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D010648:Phenylacetates / Q000032:analysis*; D012680:Sensitivity and Specificity; D000073739:Strobilurins
D000911:Antibodies, Monoclonal; D005659:Fungicides, Industrial; D006241:Haptens; D008689:Methacrylates; D010573:Pesticide Residues; D010648:Phenylacetates; D000073739:Strobilurins; C469328:kresoxim-methyl