title: Isolation and identification of phospholipid molecular species in alpha wild marine shrimp Penaeus kerathurus muscle and cephalothorax.
abstract: The concentration of TL in Penaeus kerathurus muscle and cephalothorax was 1.03+/-0.04 (75.9+/-0.8% of which was PhL) and 2.36+/-0.07% (45.5+/-0.8% of which was PhL) of the wet tissue, respectively. The phosphatidylethanolamine represented 26.4+/-0.6% (85.6% diacyl- and 14.4% alkyl-acyl- or alkenyl-acyl-analogues) of muscle and 24.7+/-0.2% (90.7% diacyl- and 9.3% alkyl-acyl- or 1-alkenyl-acyl-analogues) of cephalothorax phospholipids while the phosphatidylcholine represented 57.1+/-0.6% (86.9% diacyl- and 13.1% alkyl-acyl- or alkenyl-acyl-analogues) of muscle and 47.2+/-0.4% (89.1% diacyl- and 10.9% alkyl-acyl- or 1-alkenyl-acyl-analogues) of cephalothorax phospholipids, respectively. The main fatty acids of phosphatidylethanolamine were C16:0, C18:0, C18:1 omega-9, C20:4 omega-6, C20:5 omega-3, C22:6 omega-3 and of phosphatidylcholine were C16:0, C18:0, C18:1 omega-9, C20:4 omega-6, C20:5 omega-3. Low percentages of 2-OH C14:0 and cyclo-17:0 fatty acids were also determined. Phosphatidylethanolamine were found to contain a significantly (P<0.05) higher percentage of polyunsaturated fatty acids compared to phosphatidylcholine. The omega-3/omega-6 ratio in muscle phosphatidylethanolamine and phosphatidylcholine was significantly (P<0.05) higher to the ones of cephalothorax.
authors.affiliation: Food Chemistry Laboratory, Department of Chemistry, University of Athens, Panepistimioupolis Zographou, 15701 Athens, Greece. miniadou@chem.uoa.gr
authors.forename: Sofia
authors.initials: S
authors.lastname: Miniadis-Meimaroglou
authors.forename: Lintida
authors.initials: L
authors.lastname: Kora
authors.forename: Vassilia J
authors.initials: VJ
authors.lastname: Sinanoglou
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods; D002627:Chemistry, Physical / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008055:Lipids / Q000737:chemistry; D015233:Models, Statistical; D009132:Muscles / Q000378:metabolism*; D033561:Penaeidae; D010713:Phosphatidylcholines / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013909:Thorax / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C483858:phosphatidylethanolamine