[Application prospect of new site-specific recombination systems in gene transformation and gene stacking].
Site-specific recombination technology provides an important means for regulating transgene expression and improving efficiency of gene transformation. This technique can lead to the translocation, inversion, deletion, and integration, and the expression of the affected genes can be manipulated in different tissues and organs at different developmental periods. Using site-specific recombination system in gene transformation, we can not only acquire plenty of transformants with precise structural fidelity and faithful expression, but also understand the function of new genes efficiently. Gene stacking technology based on the site-specific recombination can produce transgenic crops with good complex traits, which will accelerate the process of research and development of elite cultivars and provide technical support for the exploration of transgenic crops in China.
Zhou
Yin
Y
Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China. ripplet0931@163.com
Wang
Yue-Ju
YJ
Wang
Ying
Y
D001483:Base Sequence; D001665:Binding Sites; D005818:Genetic Engineering / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics*; D013379:Substrate Specificity; D014170:Transformation, Genetic / Q000235:genetics; D019076:Transgenes / Q000235:genetics