Plaque-based competitive hybridization.
The authors have developed a simple, cost-saving experimental design, plaque-based competitive hybridization (PBCH), for genome-wide identification of genes differentially expressed in different tissues. PBCH offers advantages in comparison with other methods used in comparative genomics by combining the principles of differential hybridization with the subtractive hybridization. PBCH is particularly advantageous when libraries with few differences are to be analyzed. The authors demonstrate the use of PBCH by identifying 3 genes, up-regulated in the developing velvet antler of red deer (Cervus elaphus): ApoD, C011A2, and S100a1. The fidelity and sensitivity of PBCH is also shown: 1 specific clone among a library sample of 15,000 can be recognized. Possibilities for further utilizations are discussed.
Villányi
Zoltán
Z
Institute of Genetics, Agricultural Biotechnology Center, Gödöllö, Hungary.
Gyurján
István
I
Stéger
Viktor
V
Orosz
László
L
D000818:Animals; D000999:Antlers / Q000254:growth & development / Q000378:metabolism; D001345:Autoradiography; D010582:Bacteriophage lambda / Q000235:genetics; D003670:Deer / Q000235:genetics / Q000254:growth & development; D020869:Gene Expression Profiling / Q000379:methods; D015723:Gene Library; D023281:Genomics / Q000379:methods; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D010948:Viral Plaque Assay / Q000379:methods
D012333:RNA, Messenger