[Effect of Betamethasone on the cAMP of olfactory receptor neurons in rat].
OBJECTIVE
To observe the effects of Betamethasone on the cyclic adenosine monophosphate (cAMP) in olfactory receptor neurons (ORNs) and explore the possible mechanisms of the recovery of olfactory disorders by steroid treatment


METHODS
ORNs membrane was extracted and incubated with 0.1 mg/ml and 1.0 mg/ml Betamethasone. The concentrations of cAMP were measured by enzyme-linked immunosorbent assay at different times.


RESULTS
Compared with the control group, all Betamethasone groups showed differences, indicating 0.1 mg/ml (P < 0.05) and 1.0 mg/ml (P < 0.01) Betamethasone could rise the concentration of cAMP. The Betamethasone had obvious effects on cAMP production in rat ORNs and there was a dose-dependent effect. There was no difference between 5 minutes groups and 30 minutes groups.


CONCLUSIONS
Steroid hormone could enhance the production of cAMP of ORNs. Steroid hormone may thus contribute to the recovery of olfactory disorders partially, at least, through the effect on AC-cAMP in olfactory transduction.
Zhang
Cong
C
Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital University of Medical Science, Beijing 100730, China.
Wei
Yong-xiang
YX
Miao
Xu-tao
XT
Xing
Fei-hong
FH
Wang
Yang
Y
Zhan
Xiao-jun
XJ
D000818:Animals; D001623:Betamethasone / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D008564:Membrane Potentials; D018034:Olfactory Receptor Neurons / Q000187:drug effects* / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction
D001623:Betamethasone; D000242:Cyclic AMP