Nigella sativa oil for prevention of chronic cyclosporine nephrotoxicity: an experimental model.
Nephrotoxicity is the main secondary effect of cyclosporine A (CsA) treatment. The antioxidant action of Nigella sativa oil (NSO) may explain the protective effect of these agents against various hepatotoxic and nephrotoxic models in vivo and in vitro. This study was designed to investigate the possible protective effects of NSO, in prevention of chronic CsA-induced nephrotoxicity in rats. Animals were randomly divided into four experimental groups: the control group received sunflower oil, the other groups were treated with CsA (25 mg/kg/day b.w. orally) or NSO (2 ml/kg orally) or CsA + NSO, respectively. Urine and serum creatinine levels, tissue superoxide dismutase, glutathione peroxidase and catalase enzyme activities, and nitric oxide and malondialdehyde levels were measured, and histological examination was performed. In our study, CsA caused a significant deterioration in the renal function, morphology and gave rise to severe oxidative stress in the kidney. NSO significantly improved the functional and histological parameters and attenuated the oxidative stress induced by CsA. In conclusion, our study demonstrated for the first time that NSO protects kidney tissue against oxygen free radicals, preventing renal dysfunction and morphological abnormalities associated with chronic CsA administration.
Uz
Ebru
E
Department of Nephrology, Fatih University Medical School, Ankara, Turkey. ebruuz@yahoo.com
Bayrak
Omer
O
Uz
Efkan
E
Kaya
Arif
A
Bayrak
Reyhan
R
Uz
Burak
B
Turgut
Faruk Hilmi
FH
Bavbek
Nuket
N
Kanbay
Mehmet
M
Akcay
Ali
A
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D016572:Cyclosporine / Q000009:adverse effects*; D007166:Immunosuppressive Agents / Q000009:adverse effects*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D016877:Oxidants / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use; D010938:Plant Oils / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D029974:Carum
D000975:Antioxidants; D007166:Immunosuppressive Agents; D016877:Oxidants; D010936:Plant Extracts; D010938:Plant Oils; D016572:Cyclosporine; C104525:caraway oil