title: Peripheral blood cytogenetic studies in myelofibrosis: overall yield and comparison with bone marrow cytogenetic studies.
abstract: Among 59 consecutive patients with myelofibrosis (MF) in whom peripheral blood (PB) cytogenetic studies were performed, at least two analyzable metaphases (median 20, range 2-31) were obtained in 49 (81%) patients and in all 37 (100%) cases with PB myeloid progenitor cell count of 0.1 x 10(9)L(-1) or above (p=0.02). Twenty-two patients had concomitant PB and bone marrow (BM) cytogenetic studies; 6 showed similarly abnormal findings in both BM and PB. In another 2 cases, results were abnormal in BM but normal in PB; the opposite was seen in 1 case. These results suggest that PB can be considered as an alternative to BM for cytogenetic studies as currently used in MF but additional prospective studies are needed to support change in practice.
authors.affiliation: Division of Hematology, Rochester, MN, United States.
authors.forename: Kebede
authors.initials: K
authors.lastname: Hussein
authors.forename: Rhett P
authors.initials: RP
authors.lastname: Ketterling
authors.forename: Gordon W
authors.initials: GW
authors.lastname: Dewald
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Van Dyke
authors.forename: Ruben
authors.initials: R
authors.lastname: Mesa
authors.forename: Curtis A
authors.initials: CA
authors.lastname: Hanson
authors.forename: Ayalew
authors.initials: A
authors.lastname: Tefferi
mesh_terms: D000328:Adult; D001773:Blood Cells / Q000648:ultrastructure*; D001854:Bone Marrow Cells / Q000648:ultrastructure*; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D008297:Male; D008677:Metaphase; D055728:Primary Myelofibrosis / Q000175:diagnosis*