title: Assessment of biotechnologically relevant characteristics of heterologous hemoglobins in E. coli.
abstract: The use of the heterologous bacterial hemoglobin (VHb) from Vitreoscilla to enhance growth and productivity of Escherichia coli under conditions of oxygen limitation has been one of the foremost examples of metabolic engineering. Although VHb has earned its merits during the last two decades by providing enhanced physiological enhancements to organisms from all kingdoms of life, it has been the candidate of choice primarily for historical reasons. Findings made during the last years, however, suggest that hemoglobin and flavohemoglobin proteins from bacterial species other than Vitreoscilla or artificially generated mutant proteins or fusion variants of hemoglobins and flavohemoglobins may be better suited for use in biotechnological processes. This account provides guidelines for the assessment of biotechnologically relevant characteristics conferred by such novel heterologous hemoglobins and flavohemoglobins in E. coli.
authors.affiliation: Institute of Microbiology, ETH Zürich, Zürich, Switzerland.
authors.forename: Pauli T
authors.initials: PT
authors.lastname: Kallio
authors.forename: Christian J T
authors.initials: CJ
authors.lastname: Bollinger
authors.forename: Taija
authors.initials: T
authors.lastname: Koskenkorva
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Frey
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D019149:Bioreactors; D001709:Biotechnology; D015153:Blotting, Western; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D006418:Heme / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D013053:Spectrophotometry; D054793:Truncated Hemoglobins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D020589:Vitreoscilla / Q000737:chemistry / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D005182:Flavin-Adenine Dinucleotide; D006418:Heme; D010100:Oxygen