Alterations of organ histopathology and metallothionein mRNA expression in silver barb, Puntius gonionotus during subchronic cadmium exposure.
Common silver barb, Puntius gonionotus, exposed to the nominal concentration of 0.06 mg/L Cd for 60 d, were assessed for histopathological alterations (gills, liver and kidney), metal accumulation, and metallothionein (MT) mRNA expression. Fish exhibited pathological symptoms such as hypertrophy and hyperplasia of primary and secondary gill lamellae, vacuolization in hepatocytes, and prominent tubular and glomerular damage in the kidney. In addition, kidney accumulated the highest content of cadmium, more than gills and liver. Expression of MT mRNA was increased in both liver and kidney of treated fish. Hepatic MT levels remained high after fish were removed to Cd-free water. In contrast, MT expression in kidney was peaked after 28 d of treatment and drastically dropped when fish were removed to Cd-free water. The high concentrations of Cd in hepatic tissues indicated an accumulation site or permanent damage on this tissue.
Wangsongsak
Alisa
A
Departments of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Utarnpongsa
Suwimol
S
Kruatrachue
Maleeya
M
Ponglikitmongkol
Mathurose
M
Pokethitiyook
Prayad
P
Sumranwanich
Thitinun
T
D000818:Animals; D002104:Cadmium / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D003530:Cyprinidae / Q000235:genetics*; D004781:Environmental Exposure; D004784:Environmental Monitoring; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007928:Lethal Dose 50; D008668:Metallothionein / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D012333:RNA, Messenger; D002104:Cadmium; D008668:Metallothionein