A simple and fast method for quantification of ertapenem using meropenem as internal standard in human plasma in a clinical setting.
A high-performance liquid chromatography method for the determination of ertapenem in human plasma using ultraviolet detection (300 nm) was developed and validated. The method involved a simple organic protein precipitation that guarantees rapid sample preparation. By using meropenem as internal standard, it also guarantees improved precision (relative standard deviation <3.70) and accuracy (deviation from true value <6.58%). The method was linear from 0.59 to 150 microg/mL and reproducible. In conclusion, this method allows the measurement of ertapenem in human plasma and may be used for both clinical routine applications and pharmacokinetic studies.
D'Avolio
Antonio
A
Department of Infectious Diseases, University of Torino, Amedeo di Savoia Hospital, Corso Svizzera, Turin, Italy. antodav@inwind.it
Baietto
Lorena
L
De Rosa
Francesco Giuseppe
FG
Garazzino
Silvia
S
Sciandra
Mauro
M
Siccardi
Marco
M
Bonora
Stefano
S
Di Perri
Giovanni
G
D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D000077727:Ertapenem; D006801:Humans; D000077731:Meropenem; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000097:blood*; D014466:Ultraviolet Rays; D047090:beta-Lactams / Q000097:blood*
D000900:Anti-Bacterial Agents; D013845:Thienamycins; D047090:beta-Lactams; D000077731:Meropenem; D000077727:Ertapenem