title: Evaluation of the developmental competence and chromosomal compliment of mouse oocytes derived from in-vitro growth and maturation of preantral follicles.
abstract: OBJECTIVE
  To evaluate the developmental potential and aneuploidy rates of in-vitro versus in-vivo grown and matured mouse oocytes.
  
  
  METHODS
  Mice were superovulated to obtain in-vivo matured oocytes. Mouse preantral follicles were also mechanically isolated and cultured in-vitro. In-vitro fertilization (IVF) was performed and fertilization, cleavage, and morula/blastocyst formation rates were compared between groups. Cytogenetic analysis was used to compare oocyte aneuploidy rates and aneuploidy characteristics in the developing embryos.
  
  
  RESULTS
  In-vivo oocyte maturation resulted in higher IVF fertilization, cleavage, and morula/blastocyst formation rates versus in-vitro follicle culture (96.4% versus 78.5%, p<0.001; 95.3% versus 77.4%, p<0.001; 94.1% versus 76.9%, p<0.001). Total aneuploidy rates were higher in embryos derived from in-vitro matured oocytes versus those grown in-vivo (4.0% versus 1.3%, p<0.05).
  
  
  CONCLUSIONS
  Results indicate a reduced developmental competency of in-vitro matured oocytes. The data also highlight an increased susceptibility to meiotic errors in early stage follicles undergoing in vitro culture.
authors.affiliation: Andrology and IVF Laboratories, University of Utah School of Medicine, 675 Arapeen Dr. Ste 205, Salt Lake City, UT 84117, USA.
authors.forename: Lihua
authors.initials: L
authors.lastname: Liu
authors.forename: Vincent W
authors.initials: VW
authors.lastname: Aoki
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Carrell
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle; D011247:Pregnancy; D046509:Tissue Culture Techniques