title: Fluorescent speckle microscopy (FSM) of microtubules and actin in living cells.
abstract: Fluorescent speckle microscopy (FSM), a combination of conventional wide-field fluorescent light microscopy and digital imaging with a low-noise, charge-coupled device (CCD) camera, has been developed to allow visualization of assembly/disassembly dynamics, movement, and turnover of macromolecule assemblies in vivo and in vitro. FSM uses a low level of fluorescent subunits to avoid high background. This produces an image of speckled molecules that co-assemble with endogenous molecules and are followed to characterize dynamic events in living cells.
authors.affiliation: The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Clare
authors.initials: C
authors.lastname: Waterman-Storer
mesh_terms: D000199:Actins / Q000648:ultrastructure*; D000818:Animals; D002477:Cells / Q000648:ultrastructure; D019411:Clinical Laboratory Techniques; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D008870:Microtubules / Q000648:ultrastructure*; D012107:Research Design; D012815:Signal Processing, Computer-Assisted
chemical_list: D000199:Actins