title: Prevention of the neurocristopathy Treacher Collins syndrome through inhibition of p53 function.
abstract: Treacher Collins syndrome (TCS) is a congenital disorder of craniofacial development arising from mutations in TCOF1, which encodes the nucleolar phosphoprotein Treacle. Haploinsufficiency of Tcof1 perturbs mature ribosome biogenesis, resulting in stabilization of p53 and the cyclin G1-mediated cell-cycle arrest that underpins the specificity of neuroepithelial apoptosis and neural crest cell hypoplasia characteristic of TCS. Here we show that inhibition of p53 prevents cyclin G1-driven apoptotic elimination of neural crest cells while rescuing the craniofacial abnormalities associated with mutations in Tcof1 and extending life span. These improvements, however, occur independently of the effects on ribosome biogenesis; thus suggesting that it is p53-dependent neuroepithelial apoptosis that is the primary mechanism underlying the pathogenesis of TCS. Our work further implies that neuroepithelial and neural crest cells are particularly sensitive to cellular stress during embryogenesis and that suppression of p53 function provides an attractive avenue for possible clinical prevention of TCS craniofacial birth defects and possibly those of other neurocristopathies.
authors.affiliation: Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA.
authors.forename: Natalie C
authors.initials: NC
authors.lastname: Jones
authors.forename: Megan L
authors.initials: ML
authors.lastname: Lynn
authors.forename: Karin
authors.initials: K
authors.lastname: Gaudenz
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sakai
authors.forename: Kazushi
authors.initials: K
authors.lastname: Aoto
authors.forename: Jean-Phillipe
authors.initials: JP
authors.lastname: Rey
authors.forename: Earl F
authors.initials: EF
authors.lastname: Glynn
authors.forename: Lacey
authors.initials: L
authors.lastname: Ellington
authors.forename: Chunying
authors.initials: C
authors.lastname: Du
authors.forename: Jill
authors.initials: J
authors.lastname: Dixon
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dixon
authors.forename: Paul A
authors.initials: PA
authors.lastname: Trainor
mesh_terms: D000818:Animals; D017209:Apoptosis; D019521:Body Patterning; D001842:Bone and Bones / Q000002:abnormalities; D002453:Cell Cycle; D002645:Chickens; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000378:metabolism; D004622:Embryo, Mammalian / Q000002:abnormalities; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008342:Mandibulofacial Dysostosis / Q000517:prevention & control*; D051379:Mice; D008810:Mice, Inbred C57BL; D009432:Neural Crest / Q000002:abnormalities*; D046569:Neuroepithelial Cells / Q000166:cytology; D009687:Nuclear Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: C534702:CCNG1 protein, human; C534703:Ccng1 protein, mouse; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; C491352:Tcof1 protein, mouse; D016159:Tumor Suppressor Protein p53