title: Pathways change in expression during replicative aging in Saccharomyces cerevisiae.
abstract: Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair-related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.
authors.affiliation: Biology Department, Pomona College, Claremont, CA 91711, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Yiu
authors.forename: Alejandra
authors.initials: A
authors.lastname: McCord
authors.forename: Alison
authors.initials: A
authors.lastname: Wise
authors.forename: Rishi
authors.initials: R
authors.lastname: Jindal
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hardee
authors.forename: Allen
authors.initials: A
authors.lastname: Kuo
authors.forename: Michelle Yuen
authors.initials: MY
authors.lastname: Shimogawa
authors.forename: Laty
authors.initials: L
authors.lastname: Cahoon
authors.forename: Michelle
authors.initials: M
authors.lastname: Wu
authors.forename: John
authors.initials: J
authors.lastname: Kloke
authors.forename: Johanna
authors.initials: J
authors.lastname: Hardin
authors.forename: Laura L
authors.initials: LL
authors.lastname: Mays Hoopes
mesh_terms: D000375:Aging / Q000235:genetics*; D015536:Down-Regulation; D015966:Gene Expression Regulation, Fungal; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D012333:RNA, Messenger