[Investigation of canine mesenchymal stem cells differentiation to vascular endothelial cell in vitro].
To induce endothelial cell, canine bone marrow-derived mesenchymal stem cells (MSCs) were separated from bone marrow by density gradient centrifugation. The isolated MSCs were induced to form endothelial-like cell in the presence of vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and so on. These results showed that the cells uniformly took on a cobblestone morphology under the light microscope, and cell nucleolus was in the middle of the cells. The cells displayed Weibel-Palade bodies under the transmission electron microscope. vWF, a specific marker of endothelial cell was positive in the cells. The above results demonstrate that MSCs may be differentiated into endothelial cells in vitro.
Li
Qinggang
Q
Department of Nephrology, Beijing Friendship Hospital Affliating Capital University of Medical Sciences, Beijing 100050, China.
Xu
Xiuhong
X
Wang
Zhigang
Z
Liu
Wenhu
W
Li
Zhexian
Z
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D004285:Dogs; D042783:Endothelial Cells / Q000166:cytology*; D016228:Endothelial Growth Factors / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology
D016228:Endothelial Growth Factors; D042461:Vascular Endothelial Growth Factor A