title: Oncogenic hypophosphataemic osteomalacia: biomarker roles of fibroblast growth factor 23, 1,25-dihydroxyvitamin D3 and lymphatic vessel endothelial hyaluronan receptor 1.
abstract: Oncogenic osteomalacia (OOM) is characterised by tumour production of fibroblast growth factor 23 (FGF23) that results in hypophosphataemia and renal phosphate wasting, reduced 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) synthesis and osteomalacia. Here, we demonstrate the roles of serum FGF23 and 1,25(OH)2D3, together with the lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), as biomarkers for OOM. A previously well 52-year-old man presented with a 2-year history of generalised musculoskeletal pain and proximal myopathy. He had hypophosphataemia, elevated serum alkaline phosphatase activity, low serum 1,25(OH)2D3 and a reduced tubular maximum of phosphate/glomerular filtration rate. These findings indicated a diagnosis of OOM, but magnetic resonance imaging (MRI) and octreotide scintigraphy did not identify any tumours. Treatment with oral phosphate and calcitriol resolved the symptoms and biochemical abnormalities within 6 months. Four years later, he relapsed whilst on treatment with oral phosphate and calcitriol. Serum FGF23 concentration was elevated and MRI identified a 2 cm tumour within Hoffa's fat pad of the left knee. Removal of the tumour resulted in a complete resolution of symptoms and normalisation of the serum biochemical abnormalities including serum FGF23. Histology demonstrated a phosphaturic mesenchymal tumour, mixed connective tissue variant (PMTMCT), which revealed immunostaining with anti-LYVE-1 antibody and hence the presence of lymphatic vessels. Serum FGF23 and 1,25(OH)2D3 were found to be reliable biomarkers for OOM. In addition, the demonstration of lymphatics in the PMTMCT helps to distinguish this tumour from most typical benign haemangiomas.
authors.affiliation: Academic Endocrine Unit, Nuffield Department of Clinical Medicine, Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, University of Oxford, Headington, Oxford, OX3 7LJ, UK.
authors.forename: Fadil M
authors.initials: FM
authors.lastname: Hannan
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Athanasou
authors.forename: James
authors.initials: J
authors.lastname: Teh
authors.forename: Christopher L M H
authors.initials: CL
authors.lastname: Gibbons
authors.forename: Brian
authors.initials: B
authors.lastname: Shine
authors.forename: Rajesh V
authors.initials: RV
authors.lastname: Thakker
mesh_terms: D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D001859:Bone Neoplasms / Q000097:blood / Q000175:diagnosis / Q000601:surgery; D002117:Calcitriol / Q000008:administration & dosage; D018211:Chondrosarcoma, Mesenchymal / Q000097:blood / Q000175:diagnosis / Q000601:surgery; D004730:Endothelium, Vascular; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000097:blood*; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood*; D017674:Hypophosphatemia / Q000097:blood / Q000150:complications* / Q000188:drug therapy; D007717:Knee; D042601:Lymphatic Vessels; D008297:Male; D008875:Middle Aged; D010018:Osteomalacia / Q000097:blood / Q000175:diagnosis* / Q000209:etiology*; D010257:Paraneoplastic Syndromes / Q000097:blood / Q000175:diagnosis; D010710:Phosphates / Q000008:administration & dosage; D011237:Predictive Value of Tests; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D015415:Biomarkers; D014408:Biomarkers, Tumor; C000717427:FGF23 protein, human; D018960:Hyaluronan Receptors; D010710:Phosphates; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23; D000469:Alkaline Phosphatase; D002117:Calcitriol