[Change of neurocytes in acute intoxicated encephalopathy induced by 1, 2-dichloroethane after intervention with antagonists].
OBJECTIVE
To study the role of N-methyl-D-aspartate receptor and Ca(2+) in acute intoxicated encephalopathy induced by 1, 2-dichloroethane (1, 2-DCE) in vitro.


METHODS
Neurocytes of new born rats were cultured in vitro, which were administered with different doses of 1, 2-DCE, and NMDAR and Ca(2+) antagonists including Ketamine and Nimodiping respectively. The cell morphologic structures were observed under light microscope, and its proliferation was detected by Cell Counting Kit-VIII.


RESULTS
1, 2-DCE could damage the normal morphological structure of neurocytes: the cell body swelled and broke down, the karyon slurred or disappeared, the axone became shorten and thick, connection of neurocytes was reduced, the cell membrane was half-baked, injury of neurocytes became severer with the increase of the dose of 1, 2-DCE. There was no statistical difference in the proliferation of neurocytes between every 1, 2-DCE groups (P > 0.05), but there was significantly statistical difference between 1, 2-DCE groups, the control group, and the retarder groups (P < 0.01).


CONCLUSIONS
1, 2-DCE can damage the normal morphological structure of neurocytes, and the damage will become severer with the increase of the dose of 1, 2-DCE. However, the cell morphologic structures and proliferation of antagonist groups are much better than those in the 1, 2-DCE groups.
Wang
Jing
J
Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Gao
Mao-Long
ML
Shi
Ying-Tao
YT
Niu
Qiao
Q
D000818:Animals; D002118:Calcium / Q000037:antagonists & inhibitors / Q000502:physiology*; D002478:Cells, Cultured; D005025:Ethylene Dichlorides / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology*
D005025:Ethylene Dichlorides; D016194:Receptors, N-Methyl-D-Aspartate; C024565:ethylene dichloride; D002118:Calcium