title: Fine mapping of posttranslational modifications of the linker histone H1 from Drosophila melanogaster.
abstract: The linker histone H1 binds to the DNA in between adjacent nucleosomes and contributes to chromatin organization and transcriptional control. It is known that H1 carries diverse posttranslational modifications (PTMs), including phosphorylation, lysine methylation and ADP-ribosylation. Their biological functions, however, remain largely unclear. This is in part due to the fact that most of the studies have been performed in organisms that have several H1 variants, which complicates the analyses. We have chosen Drosophila melanogaster, a model organism, which has a single H1 variant, to approach the study of the role of H1 PTMs during embryonic development. Mass spectrometry mapping of the entire sequence of the protein showed phosphorylation only in the ten N-terminal amino acids, mostly at S10. For the first time, changes in the PTMs of a linker H1 during the development of a multicellular organism are reported. The abundance of H1 monophosphorylated at S10 decreases as the embryos age, which suggests that this PTM is related to cell cycle progression and/or cell differentiation. Additionally, we have found a polymorphism in the protein sequence that can be mistaken with lysine methylation if the analysis is not rigorous.
authors.affiliation: Munich Center for Integrated Protein Science CIPSM, Histone Modifications Group, Adolf-Butenandt Institute, Ludwig-Maximilians University of Munich, Munich, Germany.
authors.forename: Ana
authors.initials: A
authors.lastname: Villar-Garea
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
mesh_terms: D000818:Animals; D001665:Binding Sites; D002453:Cell Cycle; D002454:Cell Differentiation; D029721:Drosophila Proteins / Q000737:chemistry; D004331:Drosophila melanogaster / Q000737:chemistry* / Q000196:embryology; D047108:Embryonic Development; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D013058:Mass Spectrometry; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D029721:Drosophila Proteins; D006657:Histones