Preparation of basement membrane components from EHS tumors.
This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin-1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.
Kleinman
H K
HK
National Institute of Dental Research/NIH, Bethesda, Maryland, USA.
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D001672:Biocompatible Materials / Q000302:isolation & purification / Q000378:metabolism*; D003094:Collagen / Q000302:isolation & purification / Q000378:metabolism*; D024141:Collagen Type IV / Q000302:isolation & purification / Q000378:metabolism; D003584:Cytological Techniques; D004338:Drug Combinations; D007797:Laminin / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology*; D011509:Proteoglycans / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D001672:Biocompatible Materials; D024141:Collagen Type IV; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C121346:laminin 1; C059485:matrigel; D003094:Collagen