Metabolic labeling and immunoprecipitation of Drosophila proteins.
The genetics of Drosophila is a powerful tool in the analysis of mutants and mutant proteins. Cultures of cells derived from wild-type or mutant flies can be pulse labeled to biosynthetically label the proteins made by the cells. Immunoprecipitation (and subcellular fractionation) are used to characterize the expression of specific proteins.
Radhakrishna
Harish
H
Georgia Institute of Technology, Atlanta, Georgia.
D000818:Animals; D019411:Clinical Laboratory Techniques; D029721:Drosophila Proteins / Q000302:isolation & purification*; D047468:Immunoprecipitation; D007202:Indicators and Reagents; D007553:Isotope Labeling; D008660:Metabolism; D012107:Research Design
D029721:Drosophila Proteins; D007202:Indicators and Reagents