title: Influence of the stationary phase on the stability of thalidomide and comparison of different methods for the quantification of thalidomide in tablets using high-temperature liquid chromatography.
abstract: In this paper, three different HPLC methods for the quantification of thalidomide in tablets were developed and compared. The comparison of a conventional method at 30 degrees C with two high-temperature methods at 180 degrees C showed equal results. Using high-temperature HPLC (HT-HPLC), faster analysis times could be achieved. We have also focused on analyte stability and could show that the stationary phase has a pronounced effect on the on-column degradation of thalidomide at high temperatures. Virtually no degradation occurs if a polystyrene divinylbenzene column is used, whereas thalidomide is completely degraded at 180 degrees C when a carbon clad zirconium dioxide column is used.
authors.affiliation: Institute of Energy and Environmental Technology e. V. (IUTA), Bliersheimer Str. 60, D-47229 Duisburg, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Giegold
authors.forename: Maren
authors.initials: M
authors.lastname: Holzhauser
authors.forename: Thekla
authors.initials: T
authors.lastname: Kiffmeyer
authors.forename: Jochen
authors.initials: J
authors.lastname: Tuerk
authors.forename: Thorsten
authors.initials: T
authors.lastname: Teutenberg
authors.forename: Maike
authors.initials: M
authors.lastname: Rosenhagen
authors.forename: Dorothea
authors.initials: D
authors.lastname: Hennies
authors.forename: Torsten
authors.initials: T
authors.lastname: Hoppe-Tichy
authors.forename: Bernd
authors.initials: B
authors.lastname: Wenclawiak
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D013607:Tablets; D013792:Thalidomide / Q000032:analysis* / Q000737:chemistry*
chemical_list: D013607:Tablets; D013792:Thalidomide