Enantioselective gas chromatographic analysis of aqueous samples by on-line derivatisation. Application to enzymatic reactions.
A new gas chromatography (GC) method is presented for analysing both the conversion and the enantiomeric excess (e.e.) of samples from alcohol dehydrogenase reactions. The chiral compounds studied were a series of saturated, straight chain alcohols, ranging from 2-butanol to 2-heptanol. The alcohols were converted to the corresponding trifluoroacetylated derivatives by injecting trifluoroacetic anhydride onto the column shortly after injection of the aqueous samples in split-injection mode (1:100) onto a Chiraldex G-TA capillary GC column. Injecting seven hundred aqueous enzymatic reaction mixtures according to the above-mentioned procedure revealed no noticeable loss of column performance. Using the new GC method, conventional sample work-up procedures such as extraction and off-line derivatisation are eliminated and throughput of samples is significantly enhanced.
Mommers
John H M
JH
DSM Resolve, 6160 MD Geleen, The Netherlands. John.Mommers@dsm.com
de Wildeman
Stefaan M A
SM
Koolen
Wilma A F
WA
Duchateau
Alexander L L
AL
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism*; D002849:Chromatography, Gas / Q000379:methods*; D013237:Stereoisomerism
D000438:Alcohols; D000426:Alcohol Dehydrogenase