A simple kinetic model for myeloma cell culture with consideration of lysine limitation.
A simple kinetic model is developed to describe the dynamic behavior of myeloma cell growth and cell metabolism. Glucose, glutamine as well as lysine are considered as growth limiting substrates. The cell growth was restricted as soon as the extracellular lysine is exhausted and then intracellular lysine becomes a growth limiting substrate. In addition, a metabolic regulator model together with the Monod model is used to deal with the growth lag phase after inoculation or feeding. By using these models, concentrations of substrates and metabolites, as well as densities of viable and dead cells are quantitatively described. One batch cultivation and two fed-batch cultivations with pulse feeding of nutrients are used to validate the model.
Liu
Yuan-Hua
YH
Department of Automation, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Bi
Jing-Xiu
JX
Zeng
An-Ping
AP
Yuan
Jing-Qi
JQ
D001671:Biochemistry / Q000379:methods*; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D005947:Glucose / Q000737:chemistry; D005973:Glutamine / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008239:Lysine / Q000737:chemistry*; D008956:Models, Chemical; D015233:Models, Statistical; D008962:Models, Theoretical; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D005973:Glutamine; D005947:Glucose; D008239:Lysine