title: Effects of the myostatin F94L substitution on beef traits.
abstract: This study investigated the effects of a SNP in the myostatin gene (MSTN or growth differentiation factor 8, GDF8) on birth, growth, carcass, and beef quality traits in Australia (Aust.) and New Zealand (NZ). The SNP is a cytosine to adenine transversion in exon 1, causing an amino acid substitution of leucine for phenylalanine(94) (F94L). The experiment used crosses between the Jersey and Limousin breeds, with the design being a backcross using first-cross bulls of Jersey x Limousin or Limousin x Jersey breeding, mated to Jersey and Limousin cows. Progeny were genotyped for the myostatin SNP and phenotyped in Aust., with finishing on feedlot (366 calves, over 3 birth years) and in NZ with finishing on pasture (416 calves, over 2 birth years). The effect of the F94L allele (A allele) on birth and growth traits was not significant. The F94L allele in Limousin backcross calves was associated with an increase in meat weight (7.3 and 5.9% of the trait mean in Aust. and NZ, respectively, P < 0.001), and a reduction in fat depth (-13.9 and -18.7% of the trait means on live calves (600 d) and carcasses, respectively, Aust. only, P < 0.001), intramuscular fat content (-8.2% of the trait mean in Aust., P < 0.05; -7.1% in NZ, not significant), total carcass fat weight (-16.5 and -8.1% of the trait mean, Aust. and NZ; P < 0.001 and P < 0.05, respectively). Meat tenderness, pH, and cooking loss of the M. longissimus dorsi were not affected by the F94L variant. In the Jersey backcross calves, additive and dominance effects were confounded because the F94L allele was not segregating in the Jersey dams. The combined effects, however, were significant on LM area (4.4% in both Aust., P < 0.05, and NZ, P < 0.01), channel fat (-11.7%, NZ only, P < 0.01), rib fat depth (-11.2%, NZ only, P < 0.05), and carcass fat weight (-7.1%, NZ only, P < 0.05). The results provide strong evidence that this myostatin F94L variant provides an intermediate and more useful phenotype than the more severe double-muscling phenotype caused by knockout mutations in the myostatin gene.
authors.affiliation: School of Agriculture, Food and Wine, University of Adelaide, Roseworthy SA 5371 Australia.
authors.forename: A K
authors.initials: AK
authors.lastname: Esmailizadeh
authors.forename: C D K
authors.initials: CD
authors.lastname: Bottema
authors.forename: G S
authors.initials: GS
authors.lastname: Sellick
authors.forename: A P
authors.initials: AP
authors.lastname: Verbyla
authors.forename: C A
authors.initials: CA
authors.lastname: Morris
authors.forename: N G
authors.initials: NG
authors.lastname: Cullen
authors.forename: W S
authors.initials: WS
authors.lastname: Pitchford
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics*; D000818:Animals; D000831:Animals, Newborn / Q000235:genetics / Q000254:growth & development; D001823:Body Composition / Q000235:genetics*; D001947:Breeding; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D003433:Crosses, Genetic; D005260:Female; D005838:Genotype; D007930:Leucine / Q000235:genetics; D008297:Male; D008460:Meat / Q000592:standards; D018482:Muscle, Skeletal / Q000254:growth & development*; D055435:Myostatin; D010641:Phenotype; D010649:Phenylalanine / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: D055435:Myostatin; D016212:Transforming Growth Factor beta; D010649:Phenylalanine; D007930:Leucine