Mitotic spindle assembly in vitro.
The protocols in this unit describe the preparation of materials for an in vitro assay of mitotic spindle assembly in Xenopus egg extracts. Fluorochrome-labeled tubulin is used to visualize microtubule asters and spindles.
Merlie
J
J
University of California, Berkeley, Berkeley, Carolina, USA.
Heald
R
R
D000818:Animals; D002457:Cell Extracts; D003584:Cytological Techniques; D005260:Female; D066298:In Vitro Techniques; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D014981:Xenopus
D002457:Cell Extracts; D014404:Tubulin