title: A cell surface 230 kDa protein from murine melanoma involved with tumor malignancy.
abstract: Because melanoma incidence has increased at a dramatic rate, it is relevant to identify novel melanoma antigens for diagnosis and develop monoclonal antibodies recognizing such molecules. Some monoclonal antibodies (mAbs), raised against murine melanoma, identify molecules correlated with carcinogenesis. Herein, we describe a murine melanoma-associated 230 kDa molecule, expressed only in tumorigenic cell lines. Moreover, its expression is higher in more metastatic than less metastatic cells. G12F2 mAb, produced against this antigen, inhibited in vitro proliferation of both murine and human melanoma cells and enhanced in vitro complement activity. It also affected in vivo tumor growth and lung metastases formation. This 230kDa molecule represents an important target for experimental melanoma studies and may become a potential diagnostic marker for malignancy as well as a useful tool for immunotherapeutic approaches.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo 04023-901, Brazil.
authors.forename: Priscila Fraga Penteado
authors.initials: PF
authors.lastname: Mendes
authors.forename: Patrícia
authors.initials: P
authors.lastname: Xander
authors.forename: Ronni Rômulo
authors.initials: RR
authors.lastname: Novaes e Brito
authors.forename: Renato Arruda
authors.initials: RA
authors.lastname: Mortara
authors.forename: Miriam Galvonas
authors.initials: MG
authors.lastname: Jasiulionis
authors.forename: José Daniel
authors.initials: JD
authors.lastname: Lopes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003165:Complement System Proteins / Q000494:pharmacology; D006801:Humans; D008297:Male; D008545:Melanoma / Q000276:immunology*; D008546:Melanoma, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003165:Complement System Proteins