[Combined assay of soluble CD30 and hepatocyte growth factor for diagnosis of acute renal allograft rejection].
OBJECTIVE
To study the value of detection of both preoperative soluble CD30 (sCD30) and hepatocyte growth factor (HGF) level 5 days after transplantation in the diagnosis of acute rejection of renal allograft.


METHODS
Preoperative serum sCD30 levels and HGF level 5 days after transplantation were determined in 65 renal-transplant recipients using enzyme-linked immunosorbent assay. The recipients were divided according to the sCD30 levels positivity. Receiver operating characteristic (ROC) curves were used to assess the value of HGF level on day 5 posttransplantation for diagnosis of acute renal allograft rejection, and the value of combined assay of the sCD30 and HGF levels was also estimated.


RESULTS
After transplantation, 26 recipients developed graft rejection and 39 had uneventful recovery without rejection. With the cut-off value of sCD30 of 120 U/ml, the positivity rate of sCD30 was significantly higher in recipients with graft rejection than in those without (61.5% vs 17.9%, P<0.05). Recipients with acute rejection showed also significantly higher HGF levels on day 5 posttransplantation than those without rejection (P<0.05). ROC curve analysis indicated that HGF levels on day 5 posttransplantation was a good marker for diagnosis of acute renal allograft rejection, and at the cut-off value of 90 ug/L, the diagnostic sensitivity was 84.6% and specificity 76.9%. Evaluation of both the sCD30 and HGF levels significantly enhanced the diagnostic accuracy of acute graft rejection.


CONCLUSIONS
Combined assay of serum sCD30 and HGF levels offers a useful means for diagnosis of acute renal allograft rejection.
Li
Chuan-jiang
CJ
Department of Organ Transplantation, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. licj76@hotmail.com
Yu
Li-xin
LX
Xu
Jian
J
Fu
Shao-jie
SJ
Deng
Wen-feng
WF
Du
Chuan-fu
CF
Wang
Yi-bin
YB
D004797:Enzyme-Linked Immunosorbent Assay; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis*; D017228:Hepatocyte Growth Factor / Q000097:blood*; D006801:Humans; D017730:Ki-1 Antigen / Q000097:blood*; D016030:Kidney Transplantation; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous
D017730:Ki-1 Antigen; D017228:Hepatocyte Growth Factor