Photosystem II damage induced by chemically generated singlet oxygen in tobacco leaves.
In the present work, we investigated the role of chemically generated singlet oxygen, produced by photodynamic effect of rose bengal, in damaging the PSII complex in tobacco leaves in which protein synthesis-dependent repair was inhibited by infiltration with lincomycin. A 30-min exposure to low-intensity (150 micromol m(-2) s(-1)) photosynthetically active radiation (PAR) induced singlet oxygen production as detected by quenching of 3-[N-(beta-diethylaminoethyl)-N-dansyl]aminomethyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrole fluorescence in leaves infiltrated with both lincomycin and rose bengal. This light treatment caused photoinhibition of PSII, as revealed by the marked loss both of the photochemical yield and the amount of D1 protein in PSII reaction center. When rose bengal was not present in the leaves, these symptoms of photodamage were not induced by the same low-intensity PAR. However, when excitation pressure on PSII was increased to 1500 micromol m(-2) s(-1), irreversible photodamage of PSII was also observed, showing that the lincomycin treatment applied in vivo was sufficiently inhibiting protein repair. Our results show that singlet oxygen is able to cause oxidative damage in PSII directly, as suggested earlier and argue against its recently hypothesized role exclusive to inhibiting PSII protein repair (Nishiyama et al. 2006).
Hideg
Eva
E
Institute of Plant Biology , Biological Research Center, 6726 Szeged, Temesvári. krt. 62, Hungary.
Kós
Péter B
PB
Vass
Imre
I
D015151:Immunoblotting; D008027:Light; D008034:Lincomycin / Q000494:pharmacology; D010788:Photosynthesis / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D010940:Plant Proteins / Q000378:metabolism; D012395:Rose Bengal / Q000494:pharmacology; D026082:Singlet Oxygen / Q000378:metabolism*; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects
D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D026082:Singlet Oxygen; D012395:Rose Bengal; D008034:Lincomycin