[Genetic diversity and genetic effects of Myf6 gene in chickens].
The population genetic information about genetic distribution, variation and heterozygosity of Myf6 gene in five purelines and three crossbreds of chicken was analyzed using PCR-SSCP. And the genetic effects of Myf6 gene on fiber density and partial carcass traits were investigated. Only one mutation G/A at base position 47 of the whole CDS was found among individuals in each line. The distribution of Myf6 genotype strayed off the Hardy-Weinberg equilibrium and the heterozygosity was low in two purelines but not in three crossbreds. The wild-type allele A had significant positive effects on live-weight, carcass-weight, breast-muscle-weight and leg-muscle-weight (P<0.05), but had negative effects on most of meat quality traits. The A allele had additive effects of increasing 45.54 g of the live-weight, 41.03 g of the carcass-weight, 6 g of the breast-muscle-weight, 9.775 g of the leg-muscle-weight. The A allele had dominant effects of increasing 1.025 mm of the diameter of muscle fiber, -29.99 mm2 of the density of muscle fiber.
Sun
Wen-Hao
WH
College of Animal Science, Sichuan Agricultural University, Ya'an 625014, China. sun_xi_wen@163.com
Zhu
Qing
Q
Jiang
Xiao-Song
XS
Du
Hua-Rui
HR
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D014644:Genetic Variation; D006579:Heterozygote; D008460:Meat; D009132:Muscles / Q000378:metabolism; D018006:Myogenic Regulatory Factors / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational
D018006:Myogenic Regulatory Factors; C063403:myogenic factor 6