title: The netrin receptor UNC5B promotes angiogenesis in specific vascular beds.
abstract: There is emerging evidence that the canonical neural guidance factor netrin can also direct the growth of blood vessels. We deleted the gene encoding UNC5B, a receptor for the netrin family of guidance molecules, specifically within the embryonic endothelium of mice. The result is a profound structural and functional deficiency in the arterioles of the placental labyrinth, which leads first to flow reversal in the umbilical artery and ultimately to embryonic death. As this is the only detectable site of vascular abnormality in the mutant embryos, and because the phenotype cannot be rescued by a wild-type trophectoderm, we propose that UNC5B-mediated signaling is a specific and autonomous component of fetal-placental angiogenesis. Disruption of UNC5B represents a unique example of a mutation that acts solely within the fetal-placental vasculature and one that faithfully recapitulates the structural and physiological characteristics of clinical uteroplacental insufficiency. This pro-angiogenic, but spatially restricted requirement for UNC5B is not unique to murine development, as the knock-down of the Unc5b ortholog in zebrafish similarly results in the specific and highly penetrant absence of the parachordal vessel, the precursor to the lymphatic system.
authors.affiliation: Program in Human Molecular Biology and Genetics, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Sutip
authors.initials: S
authors.lastname: Navankasattusas
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Whitehead
authors.forename: Arminda
authors.initials: A
authors.lastname: Suli
authors.forename: Lise K
authors.initials: LK
authors.lastname: Sorensen
authors.forename: Amy H
authors.initials: AH
authors.lastname: Lim
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.forename: Kye Won
authors.initials: KW
authors.lastname: Park
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Wythe
authors.forename: Kirk R
authors.initials: KR
authors.lastname: Thomas
authors.forename: Chi-Bin
authors.initials: CB
authors.lastname: Chien
authors.forename: Dean Y
authors.initials: DY
authors.lastname: Li
mesh_terms: D000818:Animals; D001160:Arterioles / Q000002:abnormalities / Q000473:pathology; D001808:Blood Vessels / Q000196:embryology* / Q000378:metabolism; D020964:Embryo Loss; D004622:Embryo, Mammalian / Q000002:abnormalities / Q000098:blood supply / Q000473:pathology; D004625:Embryo, Nonmammalian / Q000166:cytology; D004727:Endothelium / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D000860:Hypoxia; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic; D000075386:Netrin Receptors; D009928:Organ Specificity; D010641:Phenotype; D010920:Placenta / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D012039:Regional Blood Flow; D015398:Signal Transduction; D014470:Umbilical Cord / Q000098:blood supply; D015027:Zebrafish / Q000196:embryology
chemical_list: D000075386:Netrin Receptors; D011956:Receptors, Cell Surface