title: Functional involvement of the organic cation transporter 2 (rOct2) in the renal uptake of organic cations in rats.
abstract: This study examined the contribution made by organic cation transporters (hOCT/rOct) to the saturable component of the renal uptake of 1-methyl-4-phenylpyridinium, tetraethylammonium (TEA), cimetidine and metformin into rOct2-expressing HEK293 cells and rat kidney slices. All the test compounds accumulated in the rat kidney slices in a carrier-mediated manner. The Michaelis- Menten constant (K(m)) values for saturable uptake of TEA, cimetidine and metformin into rat kidney slices were relatively comparable with those for the rOct2-expressing HEK293 cells. In addition, the relative uptake activity values of TEA, cimetidine and metformin in rat kidney slices were similar to those in rOct2-expressing HEK293 cells. This suggests that the saturable components involved in the renal uptake of TEA, cimetidine and metformin are mediated mainly by rOct2. The saturable uptake profile of cationic compounds into rat kidney can be evaluated in both cDNA-expressing cells and rat kidney slices, as well as the transporter expression pattern. This approach can also be used to estimate the saturable uptake mechanism of cationic compounds into the human kidney when human kidney slices and hOCT2-expressing cells are used.
authors.affiliation: Drug Metabolism Research Laboratories, Drug Discovery Research, Astellas Pharma Inc., Itabashi-ku, Tokyo, Japan. kenichi.umehara@jp.astellas.com
authors.forename: K-I
authors.initials: KI
authors.lastname: Umehara
authors.forename: T
authors.initials: T
authors.lastname: Iwatsubo
authors.forename: K
authors.initials: K
authors.lastname: Noguchi
authors.forename: H
authors.initials: H
authors.lastname: Kamimura
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000378:metabolism; D000818:Animals; D002412:Cations / Q000378:metabolism; D002460:Cell Line; D002927:Cimetidine / Q000378:metabolism; D018076:DNA, Complementary; D004847:Epithelial Cells / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D008687:Metformin / Q000378:metabolism; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D000075102:Organic Cation Transporter 2; D009930:Organic Chemicals / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019789:Tetraethylammonium / Q000378:metabolism; D014162:Transfection
chemical_list: D002412:Cations; D018076:DNA, Complementary; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; D009930:Organic Chemicals; C100590:Slc22a2 protein, rat; D019789:Tetraethylammonium; D002927:Cimetidine; D008687:Metformin; D015655:1-Methyl-4-phenylpyridinium