title: Sex identification of owls (family Strigidae) using oligonucleotide microarrays.
abstract: Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species.
authors.affiliation: Graduate Institute of Veterinary Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Lih Chiann
authors.initials: LC
authors.lastname: Wang
authors.forename: Lucia Liu
authors.initials: LL
authors.lastname: Severinghaus
authors.forename: Chi Tsong
authors.initials: CT
authors.lastname: Chen
authors.forename: Lu Yuan
authors.initials: LY
authors.lastname: Liu
authors.forename: Chu Hsiang
authors.initials: CH
authors.lastname: Pan
authors.forename: Dean
authors.initials: D
authors.lastname: Huang
authors.forename: Hsiao Yuan
authors.initials: HY
authors.lastname: Lee
authors.forename: Jihn Tsair
authors.initials: JT
authors.lastname: Lir
authors.forename: Shih Chien
authors.initials: SC
authors.lastname: Chin
authors.forename: Chang En
authors.initials: CE
authors.lastname: Pu
authors.forename: Ching Ho
authors.initials: CH
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D006579:Heterozygote; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020305:Strigiformes / Q000235:genetics*