[Formation of superoxide radicals in isolated cardiac mitochondria: effect of adriamycin].
The effect of adriamycin (doxorubicin) on superoxide radical formation in isolated rat heart mitochondria was studied by the spin trapping technique. The samples were placed into the cavity of EPR spectrometer in thin - wall gas - permeable capillary tubes, which allowed keeping the mitochondria of suspension in aerobic conditions. TIRON was used as a spin trap. We demonstrated that the rate of superoxide generation by isolated mitochondria depended radically on the presence of 1-150 microM adriamycin in incubation medium and was considerably higher than in control. The effect of adriamycin could be observed in the presence of both complex I (succinate) or complex II (glutamate and malate) substrates. The results obtained let to conclude that isolated cardiac mitochondria modified by adriamycin have a higher rate of production of superoxide radicals, which can react with spin traps not penetrating through the internal membrane.
Sviriaeva
I V
IV
Ruuge
E K
EK
Shumaev
K B
KB
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D004317:Doxorubicin / Q000633:toxicity*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D013481:Superoxides; D004317:Doxorubicin