title: A microfluidics-based turning assay reveals complex growth cone responses to integrated gradients of substrate-bound ECM molecules and diffusible guidance cues.
abstract: Neuronal growth cones contain sophisticated molecular machinery precisely regulating their migration in response to complex combinatorial gradients of diverse external cues. The details of this regulation are still largely unknown, in part due to limitations of the currently available experimental techniques. Microfluidic devices have been shown to be capable of generating complex, stable and precisely controlled chemical gradients, but their use in studying growth cone migration has been limited in part due to the effects of shear stress. Here we describe a microfluidics-based turning-assay chip designed to overcome this issue. In addition to generating precise gradients of soluble guidance cues, the chip can also fabricate complex composite gradients of diffusible and surface-bound guidance cues that mimic the conditions the growth cones realistically counter in vivo. Applying this assay to Xenopus embryonic spinal neurons, we demonstrate that the presence of a surface-bound laminin gradient can finely tune the polarity of growth cone responses (repulsion or attraction) to gradients of brain-derived neurotrophic factor (BDNF), with the guidance outcome dependent on the mean BDNF concentration. The flexibility inherent in this assay holds significant potential for refinement of our understanding of nervous system development and regeneration, and can be extended to elucidate other cellular processes involving chemotaxis of shear sensitive cells.
authors.affiliation: Department of Biomedical Engineering and Whitaker Institute of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, MD 21218, USA.
authors.forename: C
authors.initials: C
authors.lastname: Joanne Wang
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.forename: Benjamin
authors.initials: B
authors.lastname: Lin
authors.forename: Sangwoo
authors.initials: S
authors.lastname: Shim
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Ming
authors.forename: Andre
authors.initials: A
authors.lastname: Levchenko
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000737:chemistry / Q000494:pharmacology*; D004058:Diffusion; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005109:Extracellular Matrix / Q000737:chemistry*; D020439:Growth Cones / Q000187:drug effects*; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D012995:Solubility; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology; D013499:Surface Properties; D014981:Xenopus / Q000196:embryology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004129:Dimethylpolysiloxanes; D007797:Laminin