A solution for universal classification of species based on genomic DNA.
Traditionally, organisms have been classified on the basis of their phenotype. Recently, genotype-based classification has become possible through the development of sequencing technology. However, it is still difficult to apply sequencing approaches to the analysis of a large number of species due to the cost and labor. In most biological fields, the analysis of complex systems comprising various species has become an important theme, demanding an effective method for handling a vast number of species. In this paper, we have demonstrated, using plants, fish, and insects, that genome profiling, a compact technology for genome analysis, can classify organisms universally. Surprisingly, in all three of the domains of organisms tested, the phylogenetic trees generated from the phenotype topologically matched completely those generated from the genotype. Furthermore, a single probe was sufficient for the genome profiling, thereby demonstrating that this methodology is universal and compact.
Kouduka
Mariko
M
Department of Functional Materials Science, Saitama University, Japan.
Sato
Daisuke
D
Komori
Manabu
M
Kikuchi
Motohiro
M
Miyamoto
Kiyoshi
K
Kosaku
Akinori
A
Naimuddin
Mohammed
M
Matsuoka
Atsushi
A
Nishigaki
Koichi
K