Metabolic labeling of prenyl and carboxyl-methyl groups.
This unit provides protocols for prenylation and carboxy-methylation of proteins in cultured cells. These modifications often accompany fatty acid acylation. Cultured cells can be labeled biosynthetically using radiolabeled mevalonate, a precursor, to label intermediates that are incorporated as prenoids--e.g., farnesyl and geranylgeranyl. Carboxy-methylation often accompanies prenylation. The methyl group can be labeled using [(3)H-methyl]methionine.
Magee
A I
AI
National Institute of Medical Research, London, United Kingdom.
D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D008745:Methylation; D008798:Mevalonic Acid / Q000378:metabolism; D011497:Protein O-Methyltransferase / Q000378:metabolism; D017368:Protein Prenylation; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D014316:Tritium / Q000032:analysis / Q000378:metabolism
D007202:Indicators and Reagents; D011506:Proteins; D014316:Tritium; C108103:alpha-methylmethionine; D012436:S-Adenosylmethionine; D008715:Methionine; D011497:Protein O-Methyltransferase; D008798:Mevalonic Acid