[Cloning and analyzing of rice blast resistance gene Pi-ta+ allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan].
A 4,672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast resistance gene Pi-ta+ has been cloned from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan by polymerase chain reaction method. The coding region shares 99.86% and 98.78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively. There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta+ gene,compared with Pi-ta from Yashiro-mochi. Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele, because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA. Pi-ta+ allele can cause disease resistance response to rice blast pathogens in plant cells. Differences in DNA sequence, deduced amino acid sequence and antibacterial spectrum may make the Pi-ta+ allele new resistant characteristics. Finding and cloning of Pi-ta+ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources.
Geng
Xian-Sheng
XS
School of Life Science, Yunnan University, Kunming 650091, China.
Yang
Ming-Zhi
MZ
Huang
Xing-Qi
XQ
Cheng
Zai-Quan
ZQ
Fu
Jian
J
Sun
Tao
T
Li
Jun
J
D000483:Alleles; D000595:Amino Acid Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D007438:Introns / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D012275:Oryza / Q000235:genetics* / Q000502:physiology*; D010935:Plant Diseases; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D020506:Untranslated Regions / Q000235:genetics
D018076:DNA, Complementary; D020506:Untranslated Regions