Analysis of cell-cell contact mediated by Ig superfamily cell adhesion molecules.
This unit provides protocols to assay cell-cell adhesion and adhesive-dependent cellular functions mediated by calcium-independent adhesion molecules. These protocols have been developed for neural cell adhesion molecules of the Ig superfamily. However, most of the protocols allow a more general application to other categories of adhesion molecules and non-neural cells.
Sonderegger
P
P
University of Zurich, Zurich, Switzerland.
Kunz
S
S
Rader
C
C
Suter
D M
DM
Stoeckli
E T
ET
D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000145:classification / Q000302:isolation & purification / Q000502:physiology*; D002460:Cell Line; D045744:Cell Line, Tumor; D002642:Chick Embryo; D002846:Chromatography, Affinity / Q000379:methods*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D005803:Genes, Immunoglobulin; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007136:Immunoglobulins / Q000502:physiology*; D007152:Immunologic Capping; D008863:Microspheres; D005810:Multigene Family; D009101:Multiple Myeloma / Q000473:pathology; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D014162:Transfection / Q000379:methods
D015815:Cell Adhesion Molecules; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D007136:Immunoglobulins; D011993:Recombinant Fusion Proteins