[Effect of Mer overexpression on HMEC-1 cell angiogenesis and its mechanism].
OBJECTIVE
To explore anti-angiogenesis effect of Mer, a member of tyrosine kinase receptor family, and its mechanism.


METHODS
Human Mer full length plasmid was transfected into HMEC-1 cells through liposome. G418 was used to select positive clone. Expression of Mer at mRNA and protein level was detected by real-time PCR and Western-blot, respectively. Transwell and Matrigel were used to evaluate the effect of overexpressed Mer on migration and angiogenesis of HMEC-1 cells. Primary angiogenesis associated factor VEGF-A, VEGF-B, VEGF-C, VEGF-D and VEGFR-1, VEGFR-2 were screened by real-time PCR.


RESULTS
After G418 selection, the Mer expression in transfected HMEC-1 cells was increased 3.61- and 2.12 fold at mRNA and protein level, respectively. Compared with negative control, the migration of Mer-HMEC-1 was decreased (21 +/- 6 vs 36 +/- 11), and angiogenesis capability on Matrigel significantly decreased. By real-time PCR, the expression of VEGF-C and VEGFR-2 was down-regulated to 44.7% and 25.6% of the negative control.


CONCLUSIONS
Overexpressed Mer tyrosine kinase receptor can inhibit the migration and angiogenesis of HMEC-1 cells through VEGF-C/VEGFR-2 signal pathway.
Fan
Lei
L
Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Zhou
Meng-Yun
MY
Shen
Fei
F
Xie
Li-Qian
LQ
Ruan
Chang-Geng
CG
D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000378:metabolism* / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D018919:Neovascularization, Physiologic; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism; D014162:Transfection; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism; D000076202:c-Mer Tyrosine Kinase
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D042442:Vascular Endothelial Growth Factors; C097072:MERTK protein, human; D020794:Receptor Protein-Tyrosine Kinases; D040262:Receptors, Vascular Endothelial Growth Factor; D000076202:c-Mer Tyrosine Kinase