title: Regulation of magnesium homeostasis in Salmonella: Mg(2+) targets the mgtA transcript for degradation by RNase E.
abstract: Extracellular Mg(2+) controls the activation of the Salmonella PhoP/PhoQ regulatory system. One of the adaptive responses driven by PhoP/PhoQ includes the transcriptional induction of mgtA and mgtCB, which encode two P-type Mg(2+) transporters. Mg(2+) also controls mgtA expression by a riboswitch located in its 5'-untranslated region (5'UTR). In this work, it was shown that the 5'UTR of both mgtA and mgtCB is responsible for a fine-tuned Mg(2+)-dependent regulation of these genes. Evidence was also provided that the Mg(2+) riboswitch targets the mgtA transcript for degradation by RNase E when cells are grown in high Mg(2+) environments.
authors.affiliation: Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
authors.forename: Silvana V
authors.initials: SV
authors.lastname: Spinelli
authors.forename: Lucas B
authors.initials: LB
authors.lastname: Pontel
authors.forename: Eleonora
authors.initials: E
authors.lastname: García Véscovi
authors.forename: Fernando C
authors.initials: FC
authors.lastname: Soncini
mesh_terms: D020121:5' Untranslated Regions; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins; D004722:Endoribonucleases / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006706:Homeostasis; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D012475:Salmonella / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D004722:Endoribonucleases; C022537:ribonuclease E; D000251:Adenosine Triphosphatases; C490561:MgtA protein, E coli; C083836:MgtB protein, Salmonella typhimurium; C093586:MgtC protein, Salmonella typhimurium; D008274:Magnesium