title: A study on mRNA expressions of interleukin 10 during fracture healing for wound age determination.
abstract: We investigated mRNA expressions of interleukin 10 (IL-10) during fracture healing for wound age determination. Macroscopically, calluses were formed at 144 h post-injury, and thickening of the fracture edge was observed at 240 h post-injury. Slight infiltration of neutrophils and macrophages was detected at 3 h post-injury, and moderate infiltration of T and B lymphocytes was detected at 72 h post-injury. In addition, osteoblasts proliferated at 24 h post-injury. IL-10 mRNA increased immediately after the intervention and peaked at 24 h, and the expressions were detected in osteocytes before and after injury, and in osteoblasts at 3 h post-injury. Although the intensity was stable in osteocytes, that in osteoblasts was strongest at 24 h post-injury. Among inflammatory cells, the expressions were exclusively detected in macrophages. These results indicated that examinations of macroscopic and microscopic changes in fracture edges, and mRNA expressions of IL-10, would be informative for fracture healing and dating.
authors.affiliation: Department of Legal Medicine, Iwate Medical University School of Medicine, 19-1 Uchimaru, Morioka, Iwate 020-8505, Japan. mtakamiy@iwate-med.ac.jp
authors.forename: Masataka
authors.initials: M
authors.lastname: Takamiya
authors.forename: Sachiko
authors.initials: S
authors.lastname: Fujita
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Saigusa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Aoki
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D049109:Cell Proliferation; D049429:Forensic Pathology; D017102:Fracture Healing; D050723:Fractures, Bone / Q000378:metabolism* / Q000473:pathology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D009504:Neutrophils / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10