title: Subcutaneous adipose tissue-derived stem cells facilitate colonic mucosal recovery from 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats.
abstract: BACKGROUND
  Adipose tissue-derived stem cells (ADSCs) can be easily obtained from subcutaneous adipose tissue, and ADSCs can be demonstrated to display multilineage developmental plasticity. In this study, using TNBS-induced colitis rats, we show the feasibility of repairing injured intestinal mucosa with adipose tissue-derived stem cells.
  
  
  METHODS
  The subcutaneous adipose tissue of F344 rats was obtained and digested by collagenase. The digested tissue was cultured in DMEM containing 10% FBS for 1 month. ADSCs were confirmed to differentiate under appropriate conditions into various lineages of cells, including bone, neural cells, adipocytes, and epithelial cells. HGF, VEGF, TGF-beta, and adiponectin in the culture supernatants of ADSCs were determined by ELISA. ADSCs (10(7) cells) were injected into the submucosa of the colon to examine their capacity to repair intestinal mucosa injured by TNBS.
  
  
  RESULTS
  In the experimental colitis model, the injection of ADSCs facilitated colonic mucosal repair and reduced the infiltration of inflammatory cells. High levels of HGF, VEGF, and adiponectin were detected in the culture supernatants of ADSCs. Moreover, injected ADSCs distributed to several layers of the colon, and some of them differentiated into mesodermal lineage cells.
  
  
  CONCLUSIONS
  ADSCs can accelerate the regeneration of injured regions in experimental colitis. HGF, VEGF, and adiponectin might be responsible for the regeneration of injured regions in the colon.
authors.affiliation: First Department of Pathology, Kansai Medical University, Osaka, Japan.
authors.forename: Yugo
authors.initials: Y
authors.lastname: Ando
authors.forename: Muneo
authors.initials: M
authors.lastname: Inaba
authors.forename: Yutaku
authors.initials: Y
authors.lastname: Sakaguchi
authors.forename: Masanobu
authors.initials: M
authors.lastname: Tsuda
authors.forename: Guo Ke
authors.initials: GK
authors.lastname: Quan
authors.forename: Mariko
authors.initials: M
authors.lastname: Omae
authors.forename: Kazuichi
authors.initials: K
authors.lastname: Okazaki
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D052242:Adiponectin / Q000032:analysis; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D003106:Colon / Q000473:pathology*; D003424:Crohn Disease / Q000473:pathology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D017228:Hepatocyte Growth Factor / Q000032:analysis; D007413:Intestinal Mucosa / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000032:analysis; D014302:Trinitrobenzenesulfonic Acid; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D052242:Adiponectin; C066855:HGF protein, human; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; D014302:Trinitrobenzenesulfonic Acid