title: High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA).
abstract: A multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9 avian reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical origins. Comparative analysis of the MLVA results and those obtained from currently available methods including serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of discrimination, with 20 distinct patterns identified to date. The newly developed MLVA system therefore provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different origins that is suitable for molecular epidemiological studies.
authors.affiliation: Bacterial Zoonoses Unit, French Food Safety Agency (AFSSA), 94706 Maisons-Alfort cedex, France. k.laroucau@afssa.fr
authors.forename: Karine
authors.initials: K
authors.lastname: Laroucau
authors.forename: Simon
authors.initials: S
authors.lastname: Thierry
authors.forename: Fabien
authors.initials: F
authors.lastname: Vorimore
authors.forename: Kinndle
authors.initials: K
authors.lastname: Blanco
authors.forename: Erhard
authors.initials: E
authors.lastname: Kaleta
authors.forename: Richard
authors.initials: R
authors.lastname: Hoop
authors.forename: Simone
authors.initials: S
authors.lastname: Magnino
authors.forename: Daisy
authors.initials: D
authors.lastname: Vanrompay
authors.forename: Konrad
authors.initials: K
authors.lastname: Sachse
authors.forename: Garry S A
authors.initials: GS
authors.lastname: Myers
authors.forename: Patrik M
authors.initials: PM
authors.lastname: Bavoil
authors.forename: Gilles
authors.initials: G
authors.lastname: Vergnaud
authors.forename: Christine
authors.initials: C
authors.lastname: Pourcel
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D001715:Bird Diseases / Q000382:microbiology; D001717:Birds; D002691:Chlamydophila psittaci / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D016680:Genome, Bacterial; D006801:Humans; D018598:Minisatellite Repeats; D010802:Phylogeny; D009956:Psittacosis / Q000382:microbiology; D017422:Sequence Analysis, DNA / Q000379:methods*