[Antioxidant effect of total flavonoids of Hedysarum polybotry on human umbilical vein endothelial cells injury induced by hydrogen peroxide].
OBJECTIVE
To investigate protective effect of total flavonoids of Hedysarum polybotry on human umbilical vein endothelial cells injury induced by hydrogen peroxide.


METHODS
Chemical light assay was used to detect the oxygen radicals scavenging capacity of total flavonoids of Hedysarum polybotry. Lactate dehydrogenase (LDH) in the culture medium, malondialdelyde (MDA) and superoxide dismutase (SOD) in the cell were measured by spectroscopy, respectively. The degress of DNA damage was evaluated with single cell gel electrophoresis (SCGE) technique.


RESULTS
Total flavonoids of Hedysarum polybortry could scavenge oxygen radicals. After being exposed to 100 micromol/L H2O2 for 4 hours, the level of LDH and MDA was increased significantly while SOD level was decreased. However, total flavonoids of Hedysarum polybotry could decrease the content of LDH, MDA and increase SOD activity significantly and inhibit the damage of DNA.


CONCLUSIONS
Total flavonoids of Hedysarum polybotry has protective effect on human umbilical vein endothelial cells injury induced by hydrogen peroxide,which may be related to increasing the antioxidant capacity of human umbilical vein endothelial cells.
Chen
Che
C
Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China. chen72123@163.com
Dong
Jian-yong
JY
Liu
Kai
K
Liu
Yong-qi
YQ
Wang
Ya-li
YL
D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D020552:Comet Assay; D004249:DNA Damage; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase