Collagen membranes loaded with collagen-binding human PDGF-BB accelerate wound healing in a rabbit dermal ischemic ulcer model.
Studies have shown that exogenous platelet-derived growth factor-BB (PDGF-BB) could accelerate the ulcer healing, but the lack of efficient growth factor delivery system limits its clinical application. Our previous work has demonstrated that the native human PDGF-BB was added a collagen-binding domain (CBD), TKKTLRT, to develop a collagen-based PDGF targeting delivery system. Here, we showed that this CBD-fused PDGF-BB (CBD-PDGF) could bind to collagen membrane efficiently. We used the rabbit dermal ischemic ulcer model to study the effects of CBD-PDGF loaded on collagen membranes. Results revealed that this system maintained a higher concentration and stronger bioactivity of PDGF-BB on the collagen membranes and promoted the re-epithelialization of dermal ulcer wounds, the collagen deposition, and the formation of capillary lumens within the newly formed tissue area. It demonstrated that collagen membranes loaded with collagen-targeting human PDGF-BB could effectively promote ulcer healing.
Sun
Wenjie
W
Key laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Lin
Hang
H
Xie
Hua
H
Chen
Bing
B
Zhao
Wenxue
W
Han
Qianqian
Q
Zhao
Yannan
Y
Xiao
Zhifeng
Z
Dai
Jianwu
J
D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage* / Q000378:metabolism; D000818:Animals; D000077214:Becaplermin; D003094:Collagen / Q000008:administration & dosage* / Q000378:metabolism; D016503:Drug Delivery Systems; D004848:Epithelium / Q000254:growth & development; D006801:Humans; D008567:Membranes, Artificial; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage* / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis; D011817:Rabbits; D012867:Skin / Q000098:blood supply; D012883:Skin Ulcer / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects*
D043925:Angiogenesis Inducing Agents; D008567:Membranes, Artificial; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D003094:Collagen