Encoded silica colloidal crystal beads as supports for potential multiplex immunoassay.
We developed a new kind of suspension array for multiplexed immunoassays using silica colloidal crystal beads (SCCBs) as coding carriers. The monodisperse and size-controlled SCCBs were fabricated by a microfluidic device. Calcination was employed to improve the mechanical stability and lower the fluorescent background of the SCCBs. Immobilization of protein molecules on the surface of the SCCBs through chemical bonds was studied, and the modification condition was optimized to increase the detection sensitivity. Results indicated that the SCCBs as supports were more sensitive (0.92 ng/mL IgG) than the glass beads (27 ng/mL IgG) and the planar carriers (140 ng/mL IgG). A multiplex immunoassay showed the flexibility and feasibility of SCCBs array in clinical applications.
Zhao
Yuanjin
Y
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Zhao
Xiangwei
X
Sun
Cheng
C
Li
Juan
J
Zhu
Rong
R
Gu
Zhongze
Z
D003102:Colloids / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D008855:Microscopy, Electron, Scanning; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
D003102:Colloids; D007074:Immunoglobulin G; D012822:Silicon Dioxide