[Genetic diversity in 19 Chinese populations of Oncomelania hupensis (Gastropoda: Rissooidea) detected by simple sequence repeat-anchored polymerase chain reaction amplification].
OBJECTIVE
To explore the genetic diversity among populations of Oncomelania hupensis.


METHODS
Simple sequence repeat-anchored PCR method was used to amplify the genomic DNA of nineteen snail populations from 8 provinces, and the genetic diversities among these snail populations were analyzed.


RESULTS
The genetic distance D among nineteen snail populations ranged from 0 to 0.73 with average genetic distance as D 0.22 +/- 0.013. All the 19 snail populations were clustered into four groups: one group included 16 snail populations distributed throughout the Yangtze River drainage system below the Three Gorges of the river (Hunan, Hubei, Jiangxi, Anhui, and Jiangsu provinces); Snail populations from Yizhou city, Guangxi province, Fuqing city from Fujian province, and Dali city from Yunnan province belonged to an independent group respectively.


CONCLUSIONS
The distribution of Oncomelania hupensis in mainland China could be divided into four subspecies, i.e. Oncomelania hupensis hupensis (O. h. hupensis ), O. h. robertsoni, O. h. tangi , and O. h. guangxiensis.
Zhou
Yi-Biao
YB
Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China.
Zhao
Gen-Ming
GM
Wei
Jian-Guo
JG
Jiang
Qing-Wu
QW
D000818:Animals; D016000:Cluster Analysis; D049851:Gastropoda / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA