title: Novel monofunctional histidinol-phosphate phosphatase of the DDDD superfamily of phosphohydrolases.
abstract: The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In this study, we functionally assessed for the first time the monofunctional DDDD-type HolPase, which is organized in the gene cluster.
authors.affiliation: Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Republic of Korea.
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
authors.forename: Yona
authors.initials: Y
authors.lastname: Cho
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Sung Gyun
authors.initials: SG
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006642:Histidinol-Phosphatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019712:Thermococcus / Q000201:enzymology* / Q000235:genetics
chemical_list: D019843:Archaeal Proteins; D019641:DNA, Archaeal; D006642:Histidinol-Phosphatase