Purification of fibronectin.
This unit describes the purification of the multifunctional adhesive glycoprotein fibronectin from plasma or of cell-derived fibronectin from cell surfaces and from conditioned medium. Fibronectin can be used in cell adhesion and migration assays, and can be obtained in relatively high purity using simple affinity chromatography techniques.
Akiyama
S K
SK
Chapel Hill, North Carolina, USA.
D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism*; D003584:Cytological Techniques; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000097:blood* / Q000302:isolation & purification*; D006801:Humans
D017077:Culture Media, Conditioned; D005353:Fibronectins