[Genetic diversity analysis of the developed Qingke (hulless barley) varieties from the plateau regions of Sichuan Province in China revealed by SRAP markers].
Genetic diversity of 25 accessions of Qingke (hulless barley) varieties from the plateau regions of Sichuan Province, China, was analyzed by using SRAP (Sequence-related Amplified Polymorphism) markers. The results showed that 64 pairs of primer combinations produced 999 clear bands, of which 62 primer pairs (96.9%) amplified 225 polymorphic bands (22.5%). Three hundred and thirty three allelic phenotypes were amplified with an average of 5.20 alleles/primer pair. The genetic diversity ranged from 0 (me9/em14, me9/em15) to 0.8928 (me6/em18) with an average of 0.5126. The 25 accessions were classified into three major groups: A, B, and C by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Thus, it was suggested that the Sichuan hulless barleys could be used as elite germplasms to enhance the genetic background for super-hulless barley breeding.
Yang
Ping
P
College of Agronomy, Key Laboratory of Southwestern Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Ya'an 625014, China. fans1984yp@yahoo.com.cn
Liu
Xian-Jun
XJ
Liu
Xin-Chun
XC
Li
Jun
J
Wang
Xi-Wen
XW
He
Shou-Pu
SP
Li
Gang
G
Yang
Wu-Yun
WY
Feng
Zong-Yun
ZY
D000483:Alleles; D002681:China; D016000:Cluster Analysis; D005819:Genetic Markers / Q000235:genetics; D001467:Hordeum / Q000235:genetics*; D011110:Polymorphism, Genetic
D005819:Genetic Markers