Differential expression of glycosomal and mitochondrial proteins in the two major life-cycle stages of Trypanosoma brucei.
Label-free semi-quantitative differential three-dimensional liquid chromatography coupled to mass spectrometry (3D-LC-MS/MS) was used to compare the glycosomal and mitochondrial proteomes of the bloodstream- and insect-form of Trypanosoma brucei. The abundance of glycosomal marker proteins identified in the two life-cycle stages corresponded well with the relative importance of biochemical pathways present in the glycosomes of the two stages and the peptide spectral count ratios of selected enzymes were in good agreement with published data about their enzymatic specific activities. This approach proved extremely useful for the generation of large scale proteomics data for the comparison of different life-cycle stages. Several proteins involved in oxidative stress protection, sugar-nucleotide synthesis, purine salvage, nucleotide-monophosphate formation and purine-nucleotide cycle were identified as glycosomal proteins.
Vertommen
Didier
D
Hormone Research Unit, de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, B-1200 Brussels, Belgium.
Van Roy
Joris
J
Szikora
Jean-Pierre
JP
Rider
Mark H
MH
Michels
Paul A M
PA
Opperdoes
Fred R
FR
D000818:Animals; D002853:Chromatography, Liquid; D005786:Gene Expression Regulation; D008018:Life Cycle Stages; D013058:Mass Spectrometry; D008830:Microbodies / Q000378:metabolism* / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000378:metabolism*; D018512:Parasitemia / Q000469:parasitology; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D014353:Trypanosomiasis, African / Q000469:parasitology
D024101:Mitochondrial Proteins; D015800:Protozoan Proteins