title: Measuring protein interactions by optical biosensors.
abstract: In recent years, optical evanescent wave biosensors have been used to characterize protein-protein interactions, including determination of equilibrium binding constants and bimolecular rate constants. This surface binding technique can provide information about chemical on-rate constants, the lifetimes of complexes formed, and the time course of the signal. This unit provides a thorough, well-illustrated discussion of the principles of optical biosensors, experimental design, ligand immobilization, experimental data analysis, and common obstacles and possible solutions.
authors.affiliation: National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Schuck
authors.forename: Lisa F
authors.initials: LF
authors.lastname: Boyd
authors.forename: Peter S
authors.initials: PS
authors.lastname: Andersen
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D015374:Biosensing Techniques / Q000379:methods*; D007118:Immunoassay / Q000379:methods; D047468:Immunoprecipitation; D007700:Kinetics; D008954:Models, Biological; D008962:Models, Theoretical; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics
chemical_list: D000906:Antibodies; D011506:Proteins