Prostaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL-HzAM1 cells).
Prostaglandins (PGs) and other eicosanoids exert important physiological actions in insects and other invertebrates, including influencing ion transport and mediating cellular immune defense functions. Although these actions are very well documented, we have no information on the mechanisms of PGs actions in insect cells. Here we report on the outcomes of experiments designed to test our hypothesis that PGs modulate gene expression in an insect cell line established from pupal ovarian tissue of the moth Helicoverpa zea (BCIRL-HzAM1 cells). We treated cells with either PGA(1) or PGE(1) for 12 or 24h then analyzed cell lysates by 2-D electrophoresis. Analysis of the gels by densitometry revealed substantial changes in protein expression in some of the protein spots we analyzed. These spots were processed for mass spectrometric analysis by MALDI TOF/TOF, which yielded in silico protein identities for all 34 spots. The apparent changes in three of the proteins were confirmed by semi-quantative PCR, showing that the changes in mRNA expression were reflected in changes in protein expression. The 34 proteins were sorted into six categories, protein actions, lipid metabolism, signal transduction, protection, cell functions and metabolism. The findings support the hypothesis that one mechanism of PG action in insect cells is the modulation of gene expression.
Stanley
David W
DW
USDA/Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Road, Columbia, MO 65203, USA. stanleyd@missouri.edu
Goodman
Cynthia
C
An
Shiheng
S
McIntosh
Arthur
A
Song
Qisheng
Q
D000527:Alprostadil / Q000276:immunology / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000276:immunology / Q000494:pharmacology*; D002460:Cell Line / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005343:Fibrinolytic Agents / Q000276:immunology / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D019476:Insect Proteins / Q000096:biosynthesis* / Q000276:immunology; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology; D009036:Moths; D010053:Ovary / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D011454:Prostaglandins A / Q000276:immunology / Q000494:pharmacology*; D011679:Pupa / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D000998:Antiviral Agents; D005343:Fibrinolytic Agents; D019476:Insect Proteins; D011454:Prostaglandins A; D000527:Alprostadil; C100573:prostaglandin A1