title: Biomarker responses in caged rockfish (Sebastes schlegeli) from Masan Bay and Haegeumgang, South Korea.
abstract: The purpose of this study was to compare enzymatic biomarker activities in fish caged at two sites, Masan Bay (contaminated) and Haeguemgang (reference). In the present study, ethoxyresorufin O-deethylase (EROD), brain acetyl cholinesterase (bAChE), muscle acetyl cholinesterase (mAChE) and butyryl cholinesterase (mBChE) in caged rockfish (Sebastes schlegeli) were measured 0, 1, 3, 7, 14, 21 and 30 days after caging. The level of CYP1A mRNA and Protein expression was induced higher in Masan Bay at 1, 3, 7, 14 and 30 days after caging. EROD activity in the caged fish was significantly higher in Masan Bay than in Haeguemgang 3 and 7 days after caging, but not at 14 and 30 days after caging. bAChE activity was significantly inhibited at 7 and 14 days after caging in Masan Bay. However, mBChE activity was not significantly inhibited during the experiment. Taken together, the data suggest that the caged fish were exposed, at least transiently, to CYP1A inducers and ChE inhibitors, which is consistent with our previous observations.
authors.affiliation: South Sea Institute, Korean Ocean Research and Development Institute (KORDI), 391 Jangbuk-Ri Jangmonk-myon, Geoje 656-830, Republic of Korea.
authors.forename: Jee-Hyun
authors.initials: JH
authors.lastname: Jung
authors.forename: So-Jueng
authors.initials: SJ
authors.lastname: Kim
authors.forename: Taek-Kyun
authors.initials: TK
authors.lastname: Lee
authors.forename: Won Joon
authors.initials: WJ
authors.lastname: Shim
authors.forename: Seonock
authors.initials: S
authors.lastname: Woo
authors.forename: Dae-Jung
authors.initials: DJ
authors.lastname: Kim
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Han
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D019363:Cytochrome P-450 CYP1A1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005398:Fisheries / Q000295:instrumentation; D005399:Fishes / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007723:Korea; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D019363:Cytochrome P-450 CYP1A1; D000110:Acetylcholinesterase