title: Behaviour and biomarkers of oxidative stress in Gambusia holbrooki after acute exposure to widely used pharmaceuticals and a detergent.
abstract: Pharmaceuticals are continuously dispersed into the environment, as a result of human and veterinary use, and have become a relevant environmental concern. In the present study, the acute toxicity of three therapeutic agents (diazepam, clofibrate, and clofibric acid) and a detergent, sodium dodecylsulphate (SDS), to the euryhaline fish Gambusia holbrooki was evaluated. Special attention was devoted to oxidative stress parameters. G. holbrooki males, captured in the estuary of the Minho River (NW Portugal), were exposed for 96 h to the selected compounds. The following oxidative stress biomarkers were evaluated in gills and liver tissues: reduced and oxidised glutathione, lipid peroxidation, and several antioxidant enzymes, namely (1) total and selenium-dependent glutathione peroxidase (GPx), (2) glutathione reductase (GRed), (3) copper-zinc superoxide dismutase (Cu-ZnSOD) and manganese superoxide dismutase (MnSOD), and (4) glutathione-S-transferases (GSTs). In the particular case of diazepam, swimming behaviour was also evaluated. The obtained results indicate an overall diminished oxidative stress response caused by SDS and diazepam. Oxidative-based alterations were observed after exposure to clofibrate and clofibric acid, with modifications of several enzymatic activities. Diazepam caused evident behavioural changes: animals showed dark pigmentation and also abnormal postures, namely lethargy and anomalous movement.
authors.affiliation: ICBAS, Instituto de Ciências Biomédicas Abel Salazar, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia, Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal. bruno@ufp.pt
authors.forename: B
authors.initials: B
authors.lastname: Nunes
authors.forename: A R
authors.initials: AR
authors.lastname: Gaio
authors.forename: F
authors.initials: F
authors.lastname: Carvalho
authors.forename: L
authors.initials: L
authors.lastname: Guilhermino
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000009:adverse effects; D000924:Anticholesteremic Agents / Q000009:adverse effects; D001522:Behavior, Animal / Q000187:drug effects*; D015415:Biomarkers; D002995:Clofibric Acid / Q000009:adverse effects*; D003532:Cyprinodontiformes / Q000378:metabolism*; D003902:Detergents / Q000009:adverse effects; D003975:Diazepam / Q000009:adverse effects*; D005880:Gills / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects*; D014874:Water Pollutants, Chemical / Q000009:adverse effects
chemical_list: D014151:Anti-Anxiety Agents; D000924:Anticholesteremic Agents; D015415:Biomarkers; D003902:Detergents; D014874:Water Pollutants, Chemical; D012967:Sodium Dodecyl Sulfate; D002995:Clofibric Acid; D003975:Diazepam