Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode.
Simultaneous determination of lactic acid, pyruvic acid, 3-hydroxybutyric acid and acetoacetic acid for clinical monitoring of lactic acidosis and ketone body formation in human plasma (20 microL) was performed by gas chromatography-mass spectrometry in selected ion monitoring (SIM) mode after generating methoxime/tert-butyldimethylsilyl derivatives. All of the targeted carboxylic acids were detected by characteristic fragment ions, which permitted sensitive and selective identification in the presence of co-extracted free fatty acids and other acidic metabolites at much higher levels. The method was linear (r>or=0.9991), reproducible (% relative standard deviation=1.2-5.8), and accurate (% relative error=-7.2-7.6), with detection limits of 0.05-1.7 ng/mL. This rapid, accurate and selective method using minimal plasma samples (20 microL) is useful in the clinical monitoring of lactic acidosis and ketone body formation in plasma.
Paik
Man-Jeong
MJ
Metabolomic Analysis Laboratory, Institute for Neuroregeneration and Stem Cell Research, Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, South Korea.
Cho
Eun-Young
EY
Kim
Hoon
H
Kim
Kyoung-Rae
KR
Choi
Sangdun
S
Ahn
Young-Hwan
YH
Lee
Gwang
G
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007657:Ketone Bodies / Q000097:blood* / Q000737:chemistry; D019344:Lactic Acid / Q000097:blood* / Q000737:chemistry; D008297:Male; D017646:Organosilicon Compounds / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D019289:Pyruvic Acid / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results
D007657:Ketone Bodies; D017646:Organosilicon Compounds; D010091:Oximes; C009660:t-butyldimethylsilyl compounds; D019344:Lactic Acid; C005514:N,N'-monomethylenebis(pyridiniumaldoxime); D019289:Pyruvic Acid