Treatment of hyperlipoproteinemia with low density lipoprotein antibodies in rats.
OBJECTIVE
To examine whether the vaccination with low density lipoprotein (LDL) antibodies could have a protective effect on rats developed atherosclerosis through the action of atherogenic diet.


METHODS
Four groups of rats (ranged 8 and 14) were treated for 8 weeks (13 January 2007 to 9 March 2007) at the animal house, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia, with normal control diet (NC), normal control diet+LDL-Ab (NC +I), atherogenic diet (AT), and atherogenic diet + LDL-Ab (AT+I). Lipid concentrations and other different parameters were measured in serum.


RESULTS
The concentrations of total cholesterol in atherogenic rats treated with LDL-Ab (AT+I) (n=9) were significantly decreased from 5.72 +/- 0.21 mmol/l to 1.81 +/- 0.08 mmol/l (p<0.001). Low density lipoprotein-cholesterol concentrations were also significantly decreased in AT+I group (p<0.001), as it dropped by 73% from 3.06 +/- 0.09 mmol/l to 0.84 +/- 0.04 mmol/l. Phospholipids concentrations were also decreased in AT+I group (p<0.01). The concentrations of both HDL-cholesterol and triacylglycerol were not significantly different from their matched groups.


CONCLUSIONS
Results of the present study showed that treatment of atherogenic rats with LDL-Ab significantly decreased the serum concentrations of TC and LDL-C. Our findings suggest that immunization with LDL-Ab has a protective effect on artherogenic rats.
Abuelgassim
Abuelgassim O
AO
Department of Biochemistry, King Saud University, PO Box 2455, Riyadh 11451, Kingdom of Saudi Arabia. gassim@ksu.edu.sa
D000818:Animals; D050197:Atherosclerosis / Q000517:prevention & control*; D001323:Autoantibodies / Q000276:immunology*; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003627:Data Interpretation, Statistical; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D006951:Hyperlipoproteinemias / Q000628:therapy*; D007114:Immunization; D008077:Lipoproteins, LDL / Q000276:immunology*; D008297:Male; D010743:Phospholipids / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D014611:Vaccination
D001323:Autoantibodies; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol