Immunohistochemistry.
Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided.
Goldstein
Marsha
M
Bio-Reference Laboratories, Inc., Elmwood Park, New Jersey, USA.
Watkins
Simon
S
D000096:Acetone; D000818:Animals; D001710:Biotin / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding; D009195:Peroxidase / Q000378:metabolism; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism
D000096:Acetone; D001710:Biotin; D019809:Streptavidin; D009195:Peroxidase