title: Structural characterization of the cell wall D-glucans isolated from the mycelium of Botryosphaeria rhodina MAMB-05.
abstract: Three D-glucans were isolated from the mycelium of the fungus Botryosphaeria rhodina MAMB-05 by sequential extraction with hot-water and hot aqueous KOH (2% w/v) followed by ethanol precipitation. Following their purification by gel permeation chromatography on Sepharose CL-4B, the structural characteristics of the D-glucans were determined by FT-IR and 13C NMR spectroscopy and, after methylation, by GC-MS. The hot-water extract produced a fraction designated Q1A that was a beta-(1-->6)-D-glucan with the following structure: [Formula: see text] The alkaline extract, when subjected to repeated freeze-thawing, yielded two fractions: K1P (insoluble) that comprised a beta-(1-->3)-D-glucan with beta-D-glucose branches at C-6 with the structure: [Formula: see text] and K1SA (soluble) consisting of a backbone chain of alpha-(1-->4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-D-glucopyranosyl residues: [Formula: see text]
authors.affiliation: Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CP 467, CEP 19060-900, Presidente Prudente, São Paulo, Brazil. corradi@fct.unesp.br
authors.forename: Maria
authors.initials: M
authors.lastname: de Lourdes Corradi da Silva
authors.forename: Eliane K
authors.initials: EK
authors.lastname: Fukuda
authors.forename: Ana Flora D
authors.initials: AF
authors.lastname: Vasconcelos
authors.forename: Robert F H
authors.initials: RF
authors.lastname: Dekker
authors.forename: Andreza C
authors.initials: AC
authors.lastname: Matias
authors.forename: Nilson K
authors.initials: NK
authors.lastname: Monteiro
authors.forename: Marilsa S
authors.initials: MS
authors.lastname: Cardoso
authors.forename: Aneli M
authors.initials: AM
authors.lastname: Barbosa
authors.forename: Joana L M
authors.initials: JL
authors.lastname: Silveira
authors.forename: Guilherme L
authors.initials: GL
authors.lastname: Sassaki
authors.forename: Elaine R
authors.initials: ER
authors.lastname: Carbonero
mesh_terms: D001203:Ascomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005936:Glucans