Rubimetide (Met-Arg-Trp) derived from Rubisco exhibits anxiolytic-like activity via the DP1 receptor in male ddY mice.
In this study, we found that Met-Arg-Trp (rubimetide), which had been isolated as a hypotensive peptide from a pepsin-pancreatin digest of spinach ribulose bisphosphate carboxylase/oxygenase (Rubisco), has anxiolytic-like activity in the elevated plus-maze test at a dose of 0.1mg/kg (i.p.) or 1.0mg/kg (p.o.) in mice with p<0.01 and p<0.05, respectively. The anxiolytic-like activity of rubimetide (0.1mg/kg, i.p.) was blocked by BW A868C (60microg/kg, i.p.), an antagonist for the DP1 receptor, suggesting the anxiolytic-like activity of rubimetide is mediated by prostaglandin D2 and the DP1 receptor.
Zhao
Hui
H
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
Ohinata
Kousaku
K
Yoshikawa
Masaaki
M
D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010446:Peptide Fragments / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry / Q000494:pharmacology*
D014151:Anti-Anxiety Agents; D010446:Peptide Fragments; D011982:Receptors, Prostaglandin; C527097:prostanoid D receptor 1, mouse; C530457:rubimetide; D012273:Ribulose-Bisphosphate Carboxylase; D015230:Prostaglandin D2