Analysis of RNA export using Xenopus oocytes.
This unit describes a procedure for monitoring RNA export in Xenopus oocytes. The technique involves synthesizing labeled RNA in vitro and microinjecting the RNA into oocyte nuclei. Following incubation the oocytes are dissected into nuclear and cytoplasmic fractions. These samples are then processed for RNA analysis, allowing the extent of export to be quantitatively assessed.
Duricka
D
D
University of Utah, Salt Lake City, Utah, USA.
Ullman
K S
KS
D021581:Active Transport, Cell Nucleus / Q000502:physiology*; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003584:Cytological Techniques; D005260:Female; D008845:Microinjections; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000378:metabolism / Q000493:pharmacokinetics*; D014981:Xenopus
D012313:RNA