Glycolipid binding epitopes involved in adherence of the periodontitis-associated bacterium Porphyromonas gingivalis.
The ability of the periodontal pathogen Porphyromonas gingivalis to use different glycolipid structures as receptors has previously been demonstrated. The bacterium adhered to acid and nonacid glycolipids originating from human organs and to nonacid glycolipids of porcine origin. The aim of the present study was to analyze these binding epitopes by structural characterization. Glycolipid fractions with positive bacterial binding from e.g. human and porcine origin, were purified by the high performance liquid chromatography technique and thereafter used in bacterial overlay assays with (35)S-labeled P. gingivalis. Purified fractions with positive binding were structurally characterized by proton nuclear magnetic resonance spectroscopy. Complementing thin-layer chromatograms and bacterial overlay assays with pure reference glycolipid fractions and competition experiments with lactose were performed to define potential receptors. The P. gingivalis binding epitopes, including cerebrosides with nonhydroxy fatty acids, lactosylceramide with hydroxy fatty acids, sulfatides, lacto-, neolacto- and gangliotetraosylceramides, are in several instances similar to those found for other bacteria, e.g. H. pylori, H. influenzae and N. meningitidis. In addition P. gingivalis also bound to the Galalpha4Gal epitope of the globo series of glycolipids. In the future these results may be valuable for development of new treatment strategies, such as anti-adhesion therapies and vaccines specifically directed against P. gingivalis infection.
Hallén
Ulrika
U
Department of Oral Pathology, Institute of Odontology, The Sahlgrenska Academy at Göteborg University, Box 450, SE 405 30 Göteborg, Sweden.
Angström
Jonas
J
Björkner
Annika E
AE
D018829:Adhesins, Bacterial / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002855:Chromatography, Thin Layer; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism*; D006017:Glycolipids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007785:Lactose / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D012469:Salivary Glands / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013552:Swine
D018829:Adhesins, Bacterial; D000939:Epitopes; D006017:Glycolipids; D007785:Lactose