Generation of continuously growing B cell lines by epstein-barr virus transformation.
Primary cultures of cells tend to have a limited life span, which in turn limits the availability of a consistent population of cells to study. In this unit, Epstein-Barr virus produced by a marmoset cell line is used to transform B cells into a continuously growing cell line that produces no virus. These cell lines can be used as a constant source of cells for further study.
Biddison
W E
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National Institute of Neurological Disorders and Stroke, NIH, Bethesda, Maryland, USA.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000821:virology*; D002144:Callithrix; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000502:physiology*; D020031:Epstein-Barr Virus Infections; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans