The POU homeobox protein Oct-1 regulates radial glia formation downstream of Notch signaling.
Radial glia cells function as guide cells for neuronal migration and a source of neural progenitor cells, and play a crucial role for the development of the central nervous system. To date, several signals have been demonstrated to promote the formation of radial glia cells and Notch signaling is one such signal. However, the mechanism of the signaling hierarchy of radial glia developmental cascade promoted by Notch signaling still remains incomplete. Here we show that Notch signaling promotes Xenopus radial glia formation and that the Notch activation is sufficient for radial glia formation prior to neural tube closure. Moreover, we have identified Oct-1 (POU2f1), a POU transcription factor, as a downstream target of Notch signaling by microarray based screen. We demonstrate that the expression of Oct-1 in the brain is regulated by Notch signaling and that Oct-1 is sufficient and necessary for radial glia formation. Together, Oct-1 is a downstream effector of Notch signaling during radial glia formation.
Kiyota
Tomomi
T
Department of Biomedical Science, Florida State University College of Medicine, 1115 W. Call St., Tallahassee, FL 32306, USA.
Kato
Akiko
A
Altmann
Curtis R
CR
Kato
Yoichi
Y
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D054259:Neural Tube / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D050811:Octamer Transcription Factor-1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D051880:Receptors, Notch / Q000378:metabolism*; D012249:Rhombencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D015398:Signal Transduction; D014746:Vimentin / Q000235:genetics / Q000378:metabolism; D029867:Xenopus Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D050811:Octamer Transcription Factor-1; D020319:Oligodeoxyribonucleotides, Antisense; C494169:POU2F1 protein, Xenopus; D051880:Receptors, Notch; D014746:Vimentin; D029867:Xenopus Proteins