Physiological characterization and interactions of isolates in phenol-degrading aerobic granules.
This study isolated nine strains of aerobic phenol-degrading granules. These isolates (I1-I9) were characterized using 16S rRNA gene sequencing, with gamma-Proteobacteria as the dominant strains in the aerobic granules. While most strains demonstrated either high phenol-degrading capabilities or auto-aggregation capabilities, three isolates, I2, I6, and I8 showed both features. These findings contradict the previous view that auto-aggregation and phenol degradation are mutually exclusive in aerobic granules. Strains I2 and I8 independently formed single-culture aerobic granules except for I3. Anti-microbial activity test results indicated that strains I2 and I8 inhibited growth of strain I3. However, co-culturing I3 with I2 or I8 helped to form granules.
Adav
Sunil S
SS
Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Lee
Duu-Jong
DJ
D001422:Bacterial Adhesion; D001673:Biodegradation, Environmental; D018533:Biomass; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D019800:Phenol / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019800:Phenol