[Protective effect of curcumin against methotrexate-induced small intestinal damage in rats].
OBJECTIVE
To observe the changes in intestinal mucosal permeability in rats with methotrexate (MTX)-induced small intestinal damage and investigate the protective effects of curcumin.


METHODS
The experiment was carried out using 4 groups of rats, namely the normal control group, enteritis model group, sulfasalazine (SASP) group and curcumin group. With the exception of the rats in the normal control group, all rats were subjected to intraperitoneal MTX injection to induce enteritis and received subsequent daily intragastric administration of SASP (100 mg/kg), curcumin (100 mg/kg), or normal saline for 5 days. The disease activity index (DAI), colonic mucosal damage index (CMDI) and histological score (HS) of the rats were evaluated. The levels of diamine oxidase (DAO) and D-lactate were assessed using spectrophotometric assay, and myeloperoxidase (MPO) activity and intracellular adhesion molecule-1 (ICAM-1) protein expression were measured by biochemical and immunohistochemical methods, respectively.


RESULTS
Compared with the normal control group, the rats in the model group showed significantly increased DAI, CMDI and HS and levels of DAO, D-lactate, ICAM-1 and MPO. Curcumin treatment resulted in significantly decreased DAI, CMDI, HS and lowered activities of D-lactate, ICAM-1 and MPO in comparison with the model group (P<0.01).


CONCLUSIONS
MTX induces increased mucosal permeability of the small intestines in rats, and curcumin may offer protective effects against MTX-induced rat enteritis by lowering the intestinal mucosal permeability.
Song
Wei-Bing
WB
Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. swb535@126.com
Zhang
Zhen-Shu
ZS
Xiao
Bing
B
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D004751:Enteritis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D005260:Female; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008727:Methotrexate; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry
D000894:Anti-Inflammatory Agents, Non-Steroidal; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase; D006631:Amine Oxidase (Copper-Containing); D003474:Curcumin; D008727:Methotrexate