title: Isolation of lipid droplets from cells by density gradient centrifugation.
abstract: Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are embedded. This unit provides protocols for isolating lipid droplets from mammalian cells by discontinuous density gradient centrifugation.
authors.affiliation: Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA.
authors.forename: Dawn L
authors.initials: DL
authors.lastname: Brasaemle
authors.forename: Nathan E
authors.initials: NE
authors.lastname: Wolins
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005057:Eukaryotic Cells / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000302:isolation & purification*; D008322:Mammals
chemical_list: D008055:Lipids