title: Induction of differentiation of myeloid leukemia cells in primary culture in response to lithocholic acid acetate, a bile acid derivative, and cooperative effects with another differentiation inducer, cotylenin A.
abstract: Lithocholic acid (LCA) acetate induced the differentiation of human leukemia cells. Treatment with a combination of LCA acetate and cotylenin A, an inducer of the differentiation of leukemia cells, was more effective than that with LCA acetate or cotylenin A alone at inducing monocytic differentiation. LCA acetate activated mitogen-activated protein kinase (MAPK) before inducing differentiation. Cotylenin A did not activate MAPK, suggesting that cotylenin A has a different mode of action. The cooperative effects of LCA acetate and cotylenin A on inducing differentiation were, at least partly, due to the enhancement of LCA acetate-induced MAPK activation by cotylenin A.
authors.affiliation: Department of Life Science, Faculty of Medicine, Shimane University, Izumo, Shimane 693-8501, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Horie
authors.forename: Miho
authors.initials: M
authors.lastname: Akimoto
authors.forename: Hiroto
authors.initials: H
authors.lastname: Tsumura
authors.forename: Makoto
authors.initials: M
authors.lastname: Makishima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Taketani
authors.forename: Seiji
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Honma
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D017931:DNA Primers; D004224:Diterpenes / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004224:Diterpenes; C423222:cotylenin A; C502354:lithocholic acid acetate; D008095:Lithocholic Acid; D020928:Mitogen-Activated Protein Kinases