Use of polystyrene spin-coated compact discs for microimmunoassaying.
The analytical potential of polystyrene (PS) spin-coated modified compact discs (CDs) surface as platforms for the development of microarray immunoassays is presented. The surface maintained the optical characteristics of compact discs, obtaining a transparent and smooth film polymer of 70 nm thickness, the track being read (lambda 780 nm) without errors in a commercial CD reader/writer. The analytical capability of the methodology was demonstrated through an analysis of a neurotoxic compound (2560 spots per disc), reaching 0.08 microg L(-1) as limit of detection. These figures demonstrate the enormous potential of using PS spin-coated compact discs in combination with CD players as an easy-to-operate and portable device to develop lab-on-a-disc analytical applications.
Tamarit-López
Jesús
J
Departamento de Química, Instituto de Química Molecular Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n, 46071 Valencia, Spain.
Morais
Sergi
S
Puchades
Rosa
R
Maquieira
Angel
A
D002021:Buffers; D016250:Compact Disks; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D018625:Microscopy, Atomic Force; D011137:Polystyrenes / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002021:Buffers; D011137:Polystyrenes