title: Quantitative measurement of cell adhesion using centrifugal force.
abstract: In order to study the biophysical forces involved in cell-substrate (or cell-cell) adhesion, it is necessary to measure the strength of adhesion. Two questions can be addressed using the centrifugal cell adhesion assay provided in this unit: what is the ligand-receptor affinity for cells adhering at 4 degrees C and what is the strength of the ATP-dependent processes that strengthen adhesion at 37 degrees C. In both cases, the strength of adhesion is measured as the resistance to disruption in the presence of a measured centrifugal force.
authors.affiliation: Duke University, Durham, North Carolina, USA.
authors.forename: D R
authors.initials: DR
authors.lastname: McClay
authors.forename: P L
authors.initials: PL
authors.lastname: Hertzler
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002498:Centrifugation / Q000379:methods*; D008024:Ligands; D013696:Temperature
chemical_list: D015815:Cell Adhesion Molecules; D008024:Ligands; D000255:Adenosine Triphosphate