CREB-binding proteins (CBP) as a transcriptional coactivator of GATA-2.
The GATA family consists of six members, GATA 1-6. In this study, we focused on GATA-2, which is expressed predominantly in hematopoietic progenitor cells and plays the key role in keeping these cells in the undifferentiated status. CREB-binding proteins (CBP) are essential transcriptional coactivators for a large number of regulated DNA-binding transcription factors, including GATA-1. But there have been no reports on whether CBP is still a co-activator of GATA-2. Here, we used the immunoprecipitation and pull-down experiments to show that the GATA-2 and CBP were physically binding together, and clarified the binding sites CH1, CH3, CH452 and CT1430 in CBP and N-finger, C-finger and N-C-finger in GATA-2. Luciferase assay results in our experiment indicated that CBP could increase GATA-2 transcriptional activity in the dose-dependent manner. GATA-1 is mainly expressed in differentiated hematopoietic cells, but still has overlap expression with GATA-2. CBP is a coactivator of GATA-2 and GATA-1. The investigation on the mechanism that could decide whether CBP binds to GATA-2 to keep hematopoietic cells in the progenitor status or to GATA-1 to start differentiation will be a very interesting and very meaningful project in the near future.
Jiang
HuiJie
H
College of Life Sciences, Ludong University, Yantai, 264025, China.
Liu
LinDe
L
Yang
ShuDe
S
Tomomi
Takahashi
T
Toru
Nakano
N
D000818:Animals; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D050989:GATA2 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011485:Protein Binding
D050989:GATA2 Transcription Factor; C494712:Gata2 protein, mouse; D050882:CREB-Binding Protein