Failure to detect measles virus ribonucleic acid in bone cells from patients with Paget's disease.
BACKGROUND
Paget's disease is a condition of focal accelerated bone turnover. Electron-microscopy investigations of osteoclasts from pagetic lesions have identified nuclear inclusion bodies that have a similar appearance to viral nucleocapsid particles. Subsequently, RNA from several paramyxoviruses has been detected in pagetic tissue, and it was suggested that these viruses, in particular measles, might play a role in the etiology of Paget's disease. We have tested for measles virus sequences in osteoblasts and bone marrow cells collected from pagetic lesions and healthy bone.


METHODS
Bone and bone marrow samples were taken from Paget's patients and control subjects, and cells were cultured from each of these tissues. RNA was extracted from 13 osteoblast cultures and 13 cultures of bone marrow cells derived from pagetic lesions, and from 26 and 23 control osteoblast and bone marrow cultures, respectively. These samples were sourced from 22 patients with Paget's disease and 31 controls. RT-PCR-nested PCR amplification was used for the detection of the genes for the measles nucleocapsid and matrix proteins.


RESULTS
Measles virus sequences were not detected in any of the pagetic or control samples. However, measles virus sequences were identified in samples of a measles virus culture isolate included as a positive control, and in a brain sample from a patient with subacute sclerosing panencephalitis, a condition associated with chronic measles infection.


CONCLUSIONS
The results of the study do not support the hypothesis that measles virus plays a role in the pathogenesis of Paget's disease.
Matthews
Brya G
BG
Department of Medicine, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
Afzal
Muhammad A
MA
Minor
Philip D
PD
Bava
Usha
U
Callon
Karen E
KE
Pitto
Rocco P
RP
Cundy
Tim
T
Cornish
Jill
J
Reid
Ian R
IR
Naot
Dorit
D
D000135:Acid Phosphatase / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001854:Bone Marrow Cells / Q000821:virology*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008459:Measles virus / Q000302:isolation & purification*; D008875:Middle Aged; D010001:Osteitis Deformans / Q000821:virology*; D010006:Osteoblasts / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis*; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D012367:RNA, Viral; D053246:Receptor Activator of Nuclear Factor-kappa B; C505686:TNFRSF11A protein, human; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase