DNA replication timing data corroborate in silico human replication origin predictions.
We develop a wavelet-based multiscale pattern recognition methodology to disentangle the replication- from the transcription-associated compositional strand asymmetries observed in the human genome. Comparing replication skew profiles to recent high-resolution replication timing data reveals that most of the putative replication origins that border the so-identified replication domains are replicated earlier than their surroundings whereas the central regions replicate late in the S phase. We discuss the implications of this first experimental confirmation of these replication origin predictions that are likely to be early replicating and active in most tissues.
Audit
B
B
Laboratoire Joliot-Curie, ENS-Lyon, CNRS, France.
Nicolay
S
S
Huvet
M
M
Touchon
M
M
d'Aubenton-Carafa
Y
Y
Thermes
C
C
Arneodo
A
A
D000818:Animals; D004261:DNA Replication; D015894:Genome, Human; D006801:Humans; D008957:Models, Genetic; D010363:Pattern Recognition, Automated / Q000379:methods*