Purification of vitronectin.
This unit describes the purification of extracellular vitronectin from plasma by a series of affinity chromatography steps. First plasma is depleted of fibronectin and other heparin-binding proteins; the sample is then treated with urea to activate the heparin-binding activity of vitronectin. The resulting vitronectin is approximately 98% pure.
Akiyama
S K
SK
Chapel Hill, North Carolina, USA.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D019096:Vitronectin / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*
D019096:Vitronectin