title: [Experiment study of PHI on histone methylation and acetylation in Molt-4 cells].
abstract: OBJECTIVE
  To investigate the effect of phenyl-hexyl isothiocyanate (PHI) on acetylation and methylation of histone in acute lymphoblastic leukemia cell line Molt4.
  
  
  METHODS
  The inhibition of cell proliferation was observed by MTT method and clone suppression test. Apoptosis and cell cycle arrest were measured by flow cytometry. The alterations in histone acetyltransferase and acetylation and methylation of histones were detected by Western blot.
  
  
  RESULTS
  PHI could up-regulate the expression of acetyltransferase (P300/CBP), markedly induced the accumulation of acetylated histone H3, H4 and methylated histone H3 lysine 4 (H3K4), and inhibited methylation on lysine 9 of H3 (H3K9). The epigenetic regulation resulted in cell cycle arrest at G0/G1 phase, and induction of apoptosis.
  
  
  CONCLUSIONS
  PHI can modulate both histone methylation and acetylation. It may serve as a histone deacetylase inhibitor, and might be a potential novel anti-leukemia agent.
authors.affiliation: Department of Hematology, Zhangzhou Municipal Hospital, Fujian Medical University, Zhangzhou 363000, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Huang
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Ma
authors.forename: Rui-Ji
authors.initials: RJ
authors.lastname: Zhen
authors.forename: Jen-Wei
authors.initials: JW
authors.lastname: Chiao
authors.forename: De-Long
authors.initials: DL
authors.lastname: Liu
mesh_terms: D000107:Acetylation / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D044127:Epigenesis, Genetic; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008745:Methylation / Q000187:drug effects
chemical_list: D006657:Histones; D017879:Isothiocyanates; C073279:6-phenylhexyl isothiocyanate; D006655:Histone Deacetylases