title: Editing through multiple bonds: threonine detection.
abstract: In in vivo (1)H spectroscopy, the signal at 1.32 ppm is usually assigned to lactate. This resonance position is shared with threonine at physiological pH. The similarity of spectral patterns of lactate and threonine renders the separate measurement of either threonine or lactate without and even with editing technically challenging. In this study, the threonine signal was detected using a single-shot multiple-bond editing technique and quantified in vivo in both rat and human brains. A threonine concentration was estimated at 0.8 +/- 0.3 mM (mean +/- SD, n = 6) in the rat brain and at approximately 0.33 mM in the human brain.
authors.affiliation: Center for Magnetic Resonance Research and Department of Radiology, University of Minnesota, Minneapolis, MN 55455, USA. gosia@cmrr.umn.edu
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Marjanska
authors.forename: Pierre-Gilles
authors.initials: PG
authors.lastname: Henry
authors.forename: Kâmil
authors.initials: K
authors.lastname: Uğurbil
authors.forename: Rolf
authors.initials: R
authors.lastname: Gruetter
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D019047:Phantoms, Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013912:Threonine / Q000378:metabolism*
chemical_list: D007773:Lactates; D013912:Threonine