title: Characterization of the promoter region of ftsZ from Corynebacterium glutamicum and controlled overexpression of FtsZ.
abstract: Of the five promoters detected for the ftsZ gene in Corynebacterium glutamicum, three were located within the coding region of the upstream ftsQ gene and two within the intergenic ftsQ-ftsZ region. The most distant ftsZ promoter showed activity in Escherichia coli and controlled high-level transcriptional expression of ftsZ in C. glutamicum. Quantitative Western blotting showed that all five promoters were active during the exponential growth phase and down-regulated during stationary phase. This tightly controlled expression of ftsZ in C. glutamicum indicated that small changes in the amount of FtsZ protein strongly affect bacterial cell viability. The controlled overexpression of ftsZ in C. glutamicum, using the promoter of the gntK gene (PgntK), resulted in approximately 5-fold overproduction of FtsZ, an increase in cell diameter, and a highly variable localization of the protein as spirals or tangles throughout the cell. These results suggest that the intracellular concentration of FtsZ is critical for productive septum formation in C. glutamicum. Our observations provide insight into the mechanisms used by the coryneform group, which lacks actin homologs and many regulators of cell division, to control cell morphology.
authors.affiliation: Department of Molecular Biology, Area of Microbiology, Faculty of Biology, University of León, Spain.
authors.forename: Michal
authors.initials: M
authors.lastname: Letek
authors.forename: Efrén
authors.initials: E
authors.lastname: Ordóñez
authors.forename: María
authors.initials: M
authors.lastname: Fiuza
authors.forename: Pilar
authors.initials: P
authors.lastname: Honrubia-Marcos
authors.forename: José
authors.initials: J
authors.lastname: Vaquera
authors.forename: José A
authors.initials: JA
authors.lastname: Gil
authors.forename: Dolores
authors.initials: D
authors.lastname: Castro
authors.forename: Luís M
authors.initials: LM
authors.lastname: Mateos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000502:physiology*; D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria