Pathways change in expression during replicative aging in Saccharomyces cerevisiae.
Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild-type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and quantitative real-time reverse-transcription polymerase chain reaction (qPCR) has shown reproducible, statistically significant changes in messenger RNA (mRNA) of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial environmental stress response. Our findings include a pseudostationary phase, downregulation of methylation-related metabolism, increased nucleotide excision repair-related mRNA, and a strong upregulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging.
Yiu
Gloria
G
Biology Department, Pomona College, Claremont, CA 91711, USA.
McCord
Alejandra
A
Wise
Alison
A
Jindal
Rishi
R
Hardee
Jennifer
J
Kuo
Allen
A
Shimogawa
Michelle Yuen
MY
Cahoon
Laty
L
Wu
Michelle
M
Kloke
John
J
Hardin
Johanna
J
Mays Hoopes
Laura L
LL
D000375:Aging / Q000235:genetics*; D015536:Down-Regulation; D015966:Gene Expression Regulation, Fungal; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012333:RNA, Messenger