Evaluation of an ELISA to assess the intensity of Fasciola hepatica infection in cattle.
An elisa with a diagnostic sensitivity of 98 per cent and specificity of 96 per cent was evaluated as a means of assessing the intensity of Fasciola hepatica infection in cattle. A total of 294 blood samples were collected from infected cattle at a local abattoir, and the level of infection in each animal was assessed on the basis of the extent of liver pathology and the presence of flukes; 120 blood samples were also collected from uninfected cattle kept on a farm known to be free of F hepatica. The results indicated that there was a significant correlation (P<0.001) between the elisa values and the intensity of infection. Values between 15 and 28 per cent of a positive control sample indicated a low intensity of infection, values between 28 and 50 per cent indicated a medium intensity of infection and values above 50 per cent indicated a high intensity of infection.
Salimi-Bejestani
M R
MR
School of Veterinary Medicine, University of Semnan, Semnan, Iran.
Cripps
P
P
Williams
D J L
DJ
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005210:Fasciola hepatica / Q000276:immunology* / Q000302:isolation & purification; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008297:Male; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth