Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread.
Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment.
Celle
Olivier
O
Agence Française de Sécurité Sanitaire des Aliments, Les Templiers, Route des Chappes, BP 111, Sophia Antipolis 06902, France.
Blanchard
Philippe
P
Olivier
Violaine
V
Schurr
Frank
F
Cougoule
Nicolas
N
Faucon
Jean-Paul
JP
Ribière
Magali
M
D000818:Animals; D001000:Ants / Q000821:virology*; D001516:Bees / Q000821:virology*; D002908:Chronic Disease; D016679:Genome, Viral; D007304:Insect Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008925:Mites / Q000821:virology*; D010243:Paralysis / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014779:Virus Replication
D012367:RNA, Viral