title: Fluorescent labeling of yeast.
abstract: This unit describes the use of several different fluorescence methods for labeling yeast cells. It includes methods to label the vacuole, the actin cytoskeleton, and chitin deposits on cell walls (bud scars), as well as methods for visualizing specific proteins in live cells with GFP chimeras and in fixed cells by immunofluorescence.
authors.affiliation: Johns Hopkins University, Baltimore, Maryland, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Baggett
authors.forename: J D
authors.initials: JD
authors.lastname: Shaw
authors.forename: C J
authors.initials: CJ
authors.lastname: Sciambi
authors.forename: H A
authors.initials: HA
authors.lastname: Watson
authors.forename: B
authors.initials: B
authors.lastname: Wendland
mesh_terms: D000199:Actins / Q000378:metabolism; D001557:Benzenesulfonates / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004705:Endocytosis; D005452:Fluoresceins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D050296:Microbial Viability; D010590:Phalloidine / Q000378:metabolism; D021381:Protein Transport; D011726:Pyridinium Compounds / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D011796:Quinacrine / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014617:Vacuoles / Q000378:metabolism
chemical_list: D000199:Actins; D001557:Benzenesulfonates; C092350:FM 4-64; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007546:Isoquinolines; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011993:Recombinant Fusion Proteins; D012235:Rhodamines; D029701:Saccharomyces cerevisiae Proteins; D002686:Chitin; D049452:Green Fluorescent Proteins; D010590:Phalloidine; C029569:diacetyldichlorofluorescein; C007061:C.I. Fluorescent Brightening Agent 28; C017475:lucifer yellow; D011796:Quinacrine