Study of antiapoptotic gene of oral carcinoma by using Bcl-2 oncogene.
The present study was addressed to find out the expression of Bcl-2 proto-oncogene in tumor tissue derived from 24 patients with malignant oral carcinoma and oral mucosa from the same patients served as control and showed a cytoplasmic pattern of Bcl-2 immunoreactivity in basal cell layer. Fourteen of 24 (58.3%) of oral carcinoma and four adenocystic carcinoma expressed positive Bcl-2 oncogene. Well-differentiated squamous cell carcinoma showed absence of immunoreactivity. No statistically significant correlation could be demonstrated between Bcl-2 immunoreactivity and the age or sex of the patients, tumor size, and lymph node metastasis. A direct correlation between Bcl-2 immunoreactivity in G2 and G3 was statistically significant (P < 0.05). Patients with absence of or low (scores 0 or 1) Bcl-2 immunoreactive tumors manifested poorer overall survival rate in comparison with patients with moderate or high (scores 2 and 3) Bcl-2 expression, but the difference was not statistically significant. Tumors exhibited three different expressions of Bcl-2 (weak, moderate, and strong positive), compared to the mucosa of some patients affected by these tumors. No correlation was found between the histopathology of the tumors, mucosal expression, and degree of Bcl-2 expression. We propose from this study that overexpression of Bcl-2 proto-oncogene acts as a strong antiapoptotic in both squamous cells and adenocystic carcinoma may be an important molecular event on oral carcinoma to make these tumors resistant to radiotherapy and chemotherapy.
Kummoona
Raja
R
Maxillofacial Surgery, Surgical Specialties Hospital, Medical City, Baghdad, Iraq. dr_raja_kummoona@yahoo.com
Mohammad Sámi
Suha
S
Al-Kapptan
Ikpal
I
Al-Muala
Header
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics*; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D000090063:Proto-Oncogene Mas; D051025:bcl-Associated Death Protein / Q000235:genetics*
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D051025:bcl-Associated Death Protein