title: Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes.
abstract: The authors describe protocols for culture conditions in which mouse ES cells can be maintained in an undifferentiated state or committed to undergo adipocyte differentiation at a high rate and in a highly reproducible fashion. There is also a protocol for maintaining and differentiating human adult stem cells, isolated form adipose tissue and from bone marrow, into adipocytes. These culture systems provide a powerful means for studying the first step of adipose cell development and a means to investigate effects of drugs on the biology of adipocytes. There are also protocols for detection of adipocytes and analysis of their gene expression.
authors.affiliation: CNRS Université de Nice Sophia Antipolis, Nice, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Wdziekonski
authors.forename: Phi
authors.initials: P
authors.lastname: Villageois
authors.forename: Christian
authors.initials: C
authors.lastname: Dani
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D018920:Coculture Techniques; D003470:Culture Media / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D003470:Culture Media