title: New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples.
abstract: Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.
authors.affiliation: Center for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne 1011, Switzerland. gilbert.greub@chuv.ch.
authors.forename: Nicola
authors.initials: N
authors.lastname: Casson
authors.forename: Klara M
authors.initials: KM
authors.lastname: Posfay-Barbe
authors.forename: Alain
authors.initials: A
authors.lastname: Gervaix
authors.forename: Gilbert
authors.initials: G
authors.lastname: Greub
mesh_terms: D000048:Acanthamoeba / Q000382:microbiology*; D000293:Adolescent; D000818:Animals; D001988:Bronchiolitis / Q000175:diagnosis* / Q000382:microbiology; D002675:Child, Preschool; D002695:Chlamydiales / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial