title: Nedd1 expression as a marker of dynamic centrosomal localization during mouse embryonic development.
abstract: As the primary microtubule-organizing centre of the mammalian cell, the centrosome plays many important roles during cell growth and organization. This is evident across a broad range of cell types and processes, such as the proliferation, differentiation and polarity of neural cells. Additionally, given its localization and function, there are likely to be many more processes that rely on the centrosome that have not yet been characterized. Currently, little is known about centrosomal dynamics during mammalian development. In this study, we have analyzed Nedd1 protein expression to characterize the localization of the centrosome during some aspects of mouse embryogenesis. Using a Nedd1 antibody we have demonstrated the colocalization of Nedd1 with centrosomal markers. We found strong expression of Nedd1, and therefore the centrosome, in highly proliferating cells during neural development. Additionally, Nedd1 was found to have high expression in the cytoplasm of a subset of cells in the dorsal root ganglia. We have also shown a distinct, polarized centrosomal localization of Nedd1 in the developing lens, retina and other polarized tissues. This study reveals the localization of Nedd1 and the centrosome during important processes in mouse embryogenesis and provides a basis for further study into its role in development.
authors.affiliation: Hanson Institute, Institute of Medical and Veterinary Science, PO Box 14, Rundle Mall, Adelaide, SA, 5000, Australia.
authors.forename: Jantina A
authors.initials: JA
authors.lastname: Manning
authors.forename: Paul A
authors.initials: PA
authors.lastname: Colussi
authors.forename: Simon A
authors.initials: SA
authors.lastname: Koblar
authors.forename: Sharad
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D016764:Cell Polarity; D002490:Central Nervous System / Q000196:embryology / Q000378:metabolism*; D018385:Centrosome / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism; D047108:Embryonic Development; D005123:Eye / Q000196:embryology / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000196:embryology / Q000378:metabolism; D006801:Humans; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009928:Organ Specificity
chemical_list: D008869:Microtubule-Associated Proteins; C509189:NEDD1 protein, human; C086844:Nedd1 protein, mouse