[Phosphorescent microanalysis as a new technical platform for molecular diagnostics].
The authors developed a method of phosphorescent multiplex microanalysis (PHOSPHAN) as a new technological platform for a wide scope of molecular diagnostic tasks, and consider the prospects of its application in the article. PHOSPHAN combines the potential of solid-phase microplate analysis with the principle of microarray laser scanning of microzonules with biospecifically bound analyte on the surface of the bottom of microplate holes with consequent real-time registration of the phosphorescent signal. The sensitivity of the instrumental detection system is approximately 1000 molecules of Pt coproporphyrin mark in the illuminated area of scanning of 30 microns in diameter. PHOSPHAN makes it possible to detect separate microbial cells in samples and increases the sensitivity of analyte detection in microaliquots eluted from blood spots dried on paper blanks. All the key elements of this technology are protected with Russian patents.
Osin
N S
NS
Pomelova
V G
VG
Sokolov
A S
AS
Bychenkova
T A
TA
Bekman
N I
NI
Sharafudinova
T Iu
TIu
Asliian
S K
SK
Ivanovskaia
N P
NP
Laricheva
S Iu
SIu
Kanaeva
T A
TA
D006801:Humans; D007239:Infections / Q000175:diagnosis*; D008163:Luminescent Measurements / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity