title: Overview of cell fractionation.
abstract: Cell fractionation is a useful preparative and analytical method in cell biology. It is essential for analysis of composition and function of cellular compartments and it is used to prepare materials for in vitro reconstitution studies This overview discusses the basic principles of centrifugation, the instruments available, choice of media, evaluation of fractionation, and procedure optimization.
authors.affiliation: University of Virginia, Charlottesville, Virginia, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Castle
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002498:Centrifugation / Q000295:instrumentation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D004867:Equipment Design; D005057:Eukaryotic Cells / Q000648:ultrastructure; D015388:Organelles