title: Detection of secondary biomarker of met-eGH as a strategy to screen for somatotropin misuse in horseracing.
abstract: Since the Australian commercialisation of the recombinant equine growth hormone (reGH) in 1998 (EquiGen-5), Bresagen), this reGH, which differs only from eGH by an additional methionine at the N-terminal end (met-eGH), is worldwide suspected to be administered to racehorses as a doping agent. Indeed, the use of this biological drug is considered as a threat to horseracing since it acts both on growth, development or reproductive functions, and on the improvement of performances. In this work, we describe two reliable techniques based on surface plasmon resonance biosensor immunoassay (SPR-BIA) and solid-phase enzyme-linked immunosorbent assay (ELISA) as new, rapid and efficient long-term screening methods applicable to horseracing antidoping analysis. The ELISA and SPR-BIA tests were applied to octanoic acid purified IgGs from serum/plasma samples collected on two thoroughbreds treated with recombinant equine growth hormone for a period of two weeks. The first kinetic study of serum/plasma antibodies raised as a consequence of recombinant equine growth hormone administrations, which allows the detection from eight days up to 200 days after the beginning of the treatment, was performed. In order to trace the occurrence of anti-reGH antibodies in routine analysis and to monitor the animal level exposure to this forbidden molecule, a random population study was conducted on 233 post-race horses.
authors.affiliation: LABERCA, Ecole Nationale Vétérinaire de Nantes, Nantes, France. laberca@vet-nantes.fr
authors.forename: Ludovic
authors.initials: L
authors.lastname: Bailly-Chouriberry
authors.forename: Emeline
authors.initials: E
authors.lastname: Chu-Van
authors.forename: Gaud
authors.initials: G
authors.lastname: Pinel
authors.forename: Patrice
authors.initials: P
authors.lastname: Garcia
authors.forename: Marie-Agnès
authors.initials: MA
authors.lastname: Popot
authors.forename: Geneviève
authors.initials: G
authors.lastname: André-Fontaine
authors.forename: Yves
authors.initials: Y
authors.lastname: Bonnaire
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques; D015153:Blotting, Western / Q000379:methods; D004300:Doping in Sports / Q000517:prevention & control*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D013006:Growth Hormone / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood*; D008715:Methionine / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C529911:methione-equine growth hormone; D013006:Growth Hormone; D008715:Methionine