ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn2+ ions.
A new fluorescent Zn2+ indicator, namely, ICPBCZin was synthesized and the spectral profile of its free and Zn2+ bound forms was studied. The newly synthesized zinc indicator incorporates as chromophore the chromeno [3',2':3,4]pyrido[1,2a] [1,3]benzimidazole moiety and belongs to the dicarboxylate-type of zinc probes. The compound is excited with visible light, exhibits high selectivity for zinc in the presence of calcium and other common biological ions, and its Zn2+ dissociation constant is 4.0 nM. Fluorescence spectra studies of ICPBCZin indicated a clear shift in its emission wavelength maxima upon Zn2+ binding, as it belongs to the class of Photoinduced Charge Transfer (PCT) indicators, along with changes in fluorescence intensity that enable the compound to be used as a ratiometric, visible-excitable Zn2+ probe.
Roussakis
Emmanuel
E
Department of Chemistry, University of Crete, Heraklion 71003, Crete, Greece.
Voutsadaki
Styliani
S
Pinakoulaki
Eftychia
E
Sideris
Dionisia P
DP
Tokatlidis
Kostas
K
Katerinopoulos
Haralambos E
HE
D001562:Benzimidazoles / Q000138:chemical synthesis / Q000737:chemistry*; D001578:Benzopyrans / Q000138:chemical synthesis / Q000737:chemistry*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D013050:Spectrometry, Fluorescence; D015032:Zinc / Q000737:chemistry*
C533500:2-((carboxymethyl)(6-cyano-7-imino-2-methoxy-7H-chromeno(3',2'-3,4)pyrido(1,2-a)(1,3)benzimidazol-3-yl)amino)acetic acid; D001562:Benzimidazoles; D001578:Benzopyrans; D002614:Chelating Agents; D005456:Fluorescent Dyes; C508760:ICPBC compound; D015032:Zinc