Screening and breeding of high taxol producing fungi by genome shuffling.
To apply the fundamental principles of genome shuffling in breeding of taxol-producing fungi, Nodulisporium sylviform was used as starting strain in this work. The procedures of protoplast fusion and genome shuffling were studied. Three hereditarily stable strains with high taxol production were obtained by four cycles of genome shuffling. The qualitative and quantitative analysis of taxol produced was confirmed using thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and LC-MS. A high taxol producing fungus, Nodulisporium sylviform F4-26, was obtained, which produced 516.37 microg/L taxol. This value is 64.41% higher than that of the starting strain NCEU-1 and 31.52%-44.72% higher than that of the parent strains.
Zhao
Kai
K
Laboratory of Microbiology, College of Life Science, Heilongjiang University, Harbin, 150080, China.
Ping
WenXiang
W
Zhang
LiNa
L
Liu
Jun
J
Lin
Yan
Y
Jin
Tao
T
Zhou
DongPo
D
D002118:Calcium / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D016681:Genome, Fungal / Q000235:genetics*; D018021:Lithium Chloride / Q000494:pharmacology; D013058:Mass Spectrometry; D015394:Molecular Structure; D017239:Paclitaxel / Q000096:biosynthesis* / Q000737:chemistry; D011523:Protoplasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013997:Time Factors; D013098:Xylariales / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects
D018021:Lithium Chloride; D017239:Paclitaxel; D002118:Calcium