Overview of subcellular fractionation procedures for the yeast Saccharomyces cerevisiae.
This unit provides an overview of centrifugation-based fractionation procedures adapted for the yeast Saccharomyces cerevisiae. The goals, merits, limitations, and critical parameters of are discussed in order to facilitate the development of subcellular fractionation strategies. Topics include yeast cell lysate preparation, differential velocity centrifugation, density gradient centrifugation, and the analysis of subcellular fractions.
Rieder
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SE
University of California, San Diego, La Jolla, California, USA.
Emr
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SD
D015415:Biomarkers; D002021:Buffers; D002241:Carbohydrates; D002458:Cell Fractionation / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D002501:Centrifugation, Zonal / Q000379:methods; D003470:Culture Media; D005362:Ficoll; D007425:Intracellular Membranes; D007472:Iohexol; D009172:Mycology / Q000379:methods*; D015388:Organelles / Q000502:physiology / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000254:growth & development / Q000648:ultrastructure*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification; D013104:Spheroplasts / Q000648:ultrastructure; D013347:Subcellular Fractions
D015415:Biomarkers; D002021:Buffers; D002241:Carbohydrates; D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D005362:Ficoll; D007472:Iohexol