title: A biomedically enriched collection of 7000 human ORF clones.
abstract: We report the production and availability of over 7000 fully sequence verified plasmid ORF clones representing over 3400 unique human genes. These ORF clones were derived using the human MGC collection as template and were produced in two formats: with and without stop codons. Thus, this collection supports the production of either native protein or proteins with fusion tags added to either or both ends. The template clones used to generate this collection were enriched in three ways. First, gene redundancy was removed. Second, clones were selected to represent the best available GenBank reference sequence. Finally, a literature-based software tool was used to evaluate the list of target genes to ensure that it broadly reflected biomedical research interests. The target gene list was compared with 4000 human diseases and over 8500 biological and chemical MeSH classes in approximately 15 Million publications recorded in PubMed at the time of analysis. The outcome of this analysis revealed that relative to the genome and the MGC collection, this collection is enriched for the presence of genes with published associations with a wide range of diseases and biomedical terms without displaying a particular bias towards any single disease or concept. Thus, this collection is likely to be a powerful resource for researchers who wish to study protein function in a set of genes with documented biomedical significance.
authors.affiliation: Harvard Institute of Proteomics, Harvard Medical School, Cambridge, Massachusetts, USA.
authors.forename: Andreas
authors.initials: A
authors.lastname: Rolfs
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Hu
authors.forename: Lars
authors.initials: L
authors.lastname: Ebert
authors.forename: Dietmar
authors.initials: D
authors.lastname: Hoffmann
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zuo
authors.forename: Niro
authors.initials: N
authors.lastname: Ramachandran
authors.forename: Jacob
authors.initials: J
authors.lastname: Raphael
authors.forename: Fontina
authors.initials: F
authors.lastname: Kelley
authors.forename: Seamus
authors.initials: S
authors.lastname: McCarron
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Jepson
authors.forename: Binghua
authors.initials: B
authors.lastname: Shen
authors.forename: Munira M A
authors.initials: MM
authors.lastname: Baqui
authors.forename: Joseph
authors.initials: J
authors.lastname: Pearlberg
authors.forename: Elena
authors.initials: E
authors.lastname: Taycher
authors.forename: Craig
authors.initials: C
authors.lastname: DeLoughery
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoerlein
authors.forename: Bernhard
authors.initials: B
authors.lastname: Korn
authors.forename: Joshua
authors.initials: J
authors.lastname: LaBaer
mesh_terms: D003001:Cloning, Molecular; D020022:Genetic Predisposition to Disease; D006801:Humans; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA