title: Trichostatin A and nuclear reprogramming of cloned rabbit embryos.
abstract: To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Shi
authors.forename: J S
authors.initials: JS
authors.lastname: Ai
authors.forename: Y C
authors.initials: YC
authors.lastname: Ouyang
authors.forename: J C
authors.initials: JC
authors.lastname: Huang
authors.forename: Z L
authors.initials: ZL
authors.lastname: Lei
authors.forename: Q
authors.initials: Q
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Yin
authors.forename: Z M
authors.initials: ZM
authors.lastname: Han
authors.forename: Q Y
authors.initials: QY
authors.lastname: Sun
authors.forename: D Y
authors.initials: DY
authors.lastname: Chen
mesh_terms: D000107:Acetylation; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D019976:Cloning, Organism; D004622:Embryo, Mammalian / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D056572:Histone Deacetylase Inhibitors; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011817:Rabbits / Q000196:embryology*
chemical_list: D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; D008239:Lysine