Adenoviral vector mediates high expression levels of human lactoferrin in the milk of rabbits.
limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3 mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.
Han
Zeng-Sheng
ZS
Department of Biological Engineering, College of Environment and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei Province 066004, People's Republic of China.
Li
Qing-Wang
QW
Zhang
Zhi-Ying
ZY
Yu
Yong-Sheng
YS
Xiao
Bo
B
Wu
Shu-Yun
SY
Jiang
Zhong-Liang
ZL
Zhao
Hong-Wei
HW
Zhao
Rui
R
Li
Jian
J
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002460:Cell Line; D002478:Cells, Cultured; D004847:Epithelial Cells; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007781:Lactoferrin / Q000235:genetics / Q000378:metabolism*; D008321:Mammary Glands, Animal / Q000166:cytology / Q000821:virology*; D008892:Milk / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic
D011994:Recombinant Proteins; D007781:Lactoferrin