Optimization of transfection.
When embarking upon any transfection procedure, a critical first step is to optimize conditions. Every mammalian cell type has a characteristic set of requirements for optimal introduction of foreign DNA; there is a tremendous degree of variability in the transfection conditions that work, even among cell types that are very similar to one another. Often, an experimenter must screen a wide variety of cell types for a desired regulatory trait, such as an appropriate response to a particular effector molecule. It is thus helpful to have a straightforward, systematic approach to optimizing transfection efficiency. This unit provides guidelines to optimizing transfection experiments.
Rose
John K
JK
Yale University School of Medicine, New Haven, Connecticut, USA.
D000818:Animals; D041702:BALB 3T3 Cells; D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D003637:DEAE-Dextran / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D018274:Electroporation / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D013997:Time Factors; D014162:Transfection / Q000379:methods*
D002130:Calcium Phosphates; D008081:Liposomes; D004247:DNA; D003637:DEAE-Dextran; C020243:calcium phosphate