title: Neuroprotective effect of hydrogen peroxide on an in vitro model of brain ischaemia.
abstract: OBJECTIVE
  Reactive oxygen species (ROS) have been postulated to play a crucial role in the pathogenesis of ischaemia-reperfusion injury. Among these, hydrogen peroxide (H(2)O(2)) is known to be a toxic compound responsible for free-radical-dependent neuronal damage. In recent years, however, the 'bad reputation' of H(2)O(2) and other ROS molecules has changed. The aim of this study was to assess the protective role of H(2)O(2) and modification in its endogenous production on the electrophysiological and morphological changes induced by oxygen/glucose deprivation (OGD) on CA1 hippocampal neurons.
  
  
  METHODS
  Neuroprotective effects of exogenous and endogenous H(2)O(2) were determined using extracellular electrophysiological recordings of field excitatory post synaptic potentials (fEPSPs) and morphological studies in a hippocampal slice preparation. In vitro OGD was delivered by switching to an artificial cerebrospinal fluid solution with no glucose and with oxygen replaced by nitrogen.
  
  
  RESULTS
  Neuroprotection against in vitro OGD was observed in slices treated with H(2)O(2) (3 mM). The rescuing action of H(2)O(2) was mediated by catalase as pre-treatment with the catalase inhibitor 3-amino-1,2,4-triazole blocked this effect. More interestingly, we showed that an increase of the endogenous levels of H(2)O(2), due to a combination of an inhibitor of the glutathione peroxidase enzyme and addition of Cu,Zn-superoxide dismutase in the tissue bath, prevented the OGD-induced irreversible depression of fEPSPs.
  
  
  CONCLUSIONS
  Taken together, our results suggest new possible strategies to lessen the damage produced by a transient brain ischaemia by increasing the endogenous tissue level of H(2)O(2).
authors.affiliation: C.E.R.C.-S Lucia Foundation IRCCS, Rome, Italy. robert.nistico@gmail.com
authors.forename: R
authors.initials: R
authors.lastname: Nisticò
authors.forename: S
authors.initials: S
authors.lastname: Piccirilli
authors.forename: M L
authors.initials: ML
authors.lastname: Cucchiaroni
authors.forename: M
authors.initials: M
authors.lastname: Armogida
authors.forename: E
authors.initials: E
authors.lastname: Guatteo
authors.forename: C
authors.initials: C
authors.lastname: Giampà
authors.forename: F R
authors.initials: FR
authors.lastname: Fusco
authors.forename: G
authors.initials: G
authors.lastname: Bernardi
authors.forename: G
authors.initials: G
authors.lastname: Nisticò
authors.forename: N B
authors.initials: NB
authors.lastname: Mercuri
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy* / Q000503:physiopathology; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D004594:Electrophysiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000473:pathology; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018696:Neuroprotective Agents; D006861:Hydrogen Peroxide; D002374:Catalase