Induction of broad cross-subtype-specific HIV-1 immune responses by a novel multivalent HIV-1 peptide vaccine in cynomolgus macaques.
One of the major obstacles in the design of an effective vaccine against HIV-1 is its antigenic variation, which results in viral escape from the immune system. Through a bioinformatics approach, we developed an innovative multivalent HIV-1 vaccine comprised of a pool of 176 lipidated and nonlipidated peptides representing variable regions of Env and Gag proteins. The potency and breadth of the candidate vaccine against a panel of HIV-1 subtypes was evaluated in nonhuman primate (cynomolgus macaques) and humanized mouse (HLA-A2.1) models. The results demonstrate strong immunogenicity with both breadth (humoral and cellular immunity) and depth (immune recognition of widely divergent viral sequences) against heterologous HIV-1 subtypes A-F.
Azizi
Ali
A
Variation Biotechnologies, Ottawa, Ontario, Canada.
Anderson
David E
DE
Torres
José V
JV
Ogrel
Andrei
A
Ghorbani
Masoud
M
Soare
Catalina
C
Sandstrom
Paul
P
Fournier
Jocelyne
J
Diaz-Mitoma
Francisco
F
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology*; D015683:Gene Products, gag / Q000008:administration & dosage / Q000276:immunology; D015483:HIV Antibodies / Q000096:biosynthesis*; D015699:HIV Envelope Protein gp120 / Q000008:administration & dosage / Q000276:immunology; D015497:HIV-1 / Q000145:classification* / Q000276:immunology*; D050356:Lipid Metabolism / Q000276:immunology; D008252:Macaca fascicularis; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000138:chemical synthesis / Q000276:immunology*
D016915:AIDS Vaccines; D000939:Epitopes; D015683:Gene Products, gag; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D014613:Vaccines, Attenuated; D022223:Vaccines, Subunit