Two-photon fluorescence microscopy with a diode-pumped Cr:LiSAF laser.
We demonstrate the acquisition of high-quality two-photon fluorescence microscopy images using an all-solid-state self-mode-locked Cr:LiSAF laser. We contrast the performance of the two-photon technique with single-photon confocal fluorescence microscopy images taken with an argon-ion laser. Examples of improved depth penetration and reduced dye bleaching are presented.
Robertson
G
G
Armstrong
D
D
Dymott
M J
MJ
Ferguson
A I
AI
Hogg
G L
GL