Design and use of fluorescent fusion proteins in cell biology.
This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.
Snapp
Erik
E
Albert Einstein College of Medicine, Bronx, New York.
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D015415:Biomarkers / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis* / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins