The VP7 genes of two G9 rotaviruses isolated in 1980 from diarrheal stool samples collected in Washington, DC, are unique molecularly and serotypically.
In a retrospective study of archival diarrheal stool samples collected from 1974 to 1991 at Children's Hospital National Medical Center, Washington, DC, we detected three genotype G9P[8] viruses in specimens collected in 1980, which represented the earliest human G9 viruses ever isolated. The VP7 genes of two culture-adapted 1980 G9 viruses were phylogenetically related closely to the lineage 2 G9 virus VP7 gene. Unexpectedly, however, the VP7s of the 1980 G9 viruses were more closely related serotypically to lineage 3 VP7s than to lineage 2 VP7, which may be supported by amino acid sequence analyses of the VP7 proteins.
Cao
Dianjun
D
Epidemiology Section, Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6308, Bethesda, MD 20902, USA.
Santos
Norma
N
Jones
Ronald W
RW
Tatsumi
Masatoshi
M
Gentsch
Jon R
JR
Hoshino
Yasutaka
Y
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology*; D003967:Diarrhea / Q000821:virology; D004219:District of Columbia; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012703:Serotyping
D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus