title: U-HO1, a new cell line derived from a primary refractory classical Hodgkin lymphoma.
abstract: The Hodgkin cell line U-HO1 was established from a malignant pleural effusion of a 23-year-old male patient during the end stage of refractory nodular sclerosing classical Hodgkin lymphoma (cHL). Since its establishment in 2005, U-HO1 has maintained stable characteristics in vitro and has a doubling time of about 4 days under standard culture conditions. U-HO1 forms typical Reed-Sternberg cells in suspension, is EBV negative, lacks HLA-A, -B, -C but expresses HLA-D proteins/CD74 and exposes CD15 together with CD30 in the absence of CD19 and CD20 on the cell surface. Karyotype analysis of U-HO1 revealed a hyperdiploid karyotype with multiple clonal aberrations. Most significant is an elongated chromosome 2, der(2)t(2;10)(q35; q16.1)add(2)(p13). CGH analysis revealed the following imbalances: ish cgh dim(1)(p13p31)(p12q21), enh(2)(p13p23), dim(4)(q31.3qter), enh(6)(q22q27), enh(12), enh(18), enh(20) (q13.1pter). FISH analysis showed about six-fold amplification of REL and BCL11A, thus, U-HO1 is prototypical for cHL in every aspect tested so far. As an outstanding feature compared to the existing HL cell lines, U-HO1 has high levels of microRNA transcripts of MIRN216 and MIRN217 located in the amplicon 2p16. Compared to other HL cell lines, U-HO1 proved far less genetically aberrant suggesting that U-HO1's imbalances suffice to cause the full-blown phenotype of primary refractory cHL.
authors.affiliation: Happareute, Rothenbach, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Mader
authors.forename: S
authors.initials: S
authors.lastname: Bruderlein
authors.forename: S
authors.initials: S
authors.lastname: Wegener
authors.forename: I
authors.initials: I
authors.lastname: Melzner
authors.forename: S
authors.initials: S
authors.lastname: Popov
authors.forename: H K
authors.initials: HK
authors.lastname: Muller-Hermelink
authors.forename: T F
authors.initials: TF
authors.lastname: Barth
authors.forename: A
authors.initials: A
authors.lastname: Viardot
authors.forename: P
authors.initials: P
authors.lastname: Moller
mesh_terms: D000328:Adult; D002460:Cell Line; D002871:Chromosome Banding; D002889:Chromosomes, Human, Pair 2; D015972:Gene Expression Regulation, Neoplastic; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006689:Hodgkin Disease / Q000235:genetics / Q000473:pathology*; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000235:genetics
chemical_list: D012322:RNA Precursors