Immunostimulatory activities of polysaccharides from liquid culture of pine-mushroom Tricholoma matsutake.
Mushrooms are regarded as one of the well-known foods and biopharmaceutical materials with a great deal of interest. Polysaccharide beta-glucan is the major component of mushrooms that displays various biological activities such as antidiabetic, anticancer, and antihyperlipidemic effects. In this study, we compared the immunostimulatory potency of polysaccharide fractions, prepared from liquid culture of pinemushroom Tricholoma matsutake, with a potent immunogen lipopolysaccharide (LPS), and their molecular mechanisms on the functional activation of macrophages. We found that fraction II (TMF-II) was able to comparably upregulate or highly enhance the phenotypic functions of macrophages such NO production and cytokine (IL-1beta, IL-6, IL-12, and TNF-alpha) expression, to LPS. TMF-II triggered the phosphorylation of IkappaBalpha, a critical step for NF-kappaB activation and translocation. Of the upstream signaling enzymes tested, Src and Akt were thought to be the responsible upstream signaling components in induction of NO production, although TMF-II strongly upregulated the phosphorylation of all MAPK pathways. Therefore, our data suggest that T. matsutake-derived beta-glucan may exert its immunostimulating activities with similar potency to LPS via activation of multiple signaling pathways linked to NF-kappaB activation.
Kim
Joo Young
JY
School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Byeon
Se Eun
SE
Lee
Yong Gyu
YG
Lee
Ji Yeon
JY
Park
Jongsun
J
Hong
Eock Ki
EK
Cho
Jae Youl
JY
D000363:Agaricales / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D015398:Signal Transduction; D020298:U937 Cells; D015854:Up-Regulation; D047071:beta-Glucans / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology
D016207:Cytokines; D016328:NF-kappa B; D011134:Polysaccharides; D047071:beta-Glucans; D009569:Nitric Oxide