Changes of gamma-tubulin expression and distribution in the zebrafish (Danio rerio) ovary, oocyte and embryo.
The tubulin gene family is important for individual zebrafish development from the oocyte through to hatching. This involves often rapid, complex changes in the gametes and embryonic cells that are reflected in underlying gene expression changes. Tubulin dynamics, i.e., the interchange of polymeric and soluble forms in zebrafish oogenesis and embryogenesis, is important for microtubule (MT) cellular functions. Nevertheless, our understanding of how tubulin gene expression changes during zebrafish development is not clear. Previous data showed that soluble alpha-tubulin and gamma-tubulin are associated with large molecular weight complexes (>2MDa) which are reduced by the blastula stage, with a concomitant decrease in soluble tubulin amount. Complexes (<2MDa) then increased in the gastrula with an increase in soluble tubulin. Microarray revealed similar patterns of tubulin gene product expression for zebrafish ovary and eggs while both differed from day 4 larva. In situ hybridization with gamma-tubulin oligonucleotide probes revealed diffuse label in oocytes, with a marked localization to the primordial blastodisc upon maturation. These findings, together with recent work on gamma-tubulin ring complexes in other species, suggest that gamma-tubulin (protein complexes) may be involved in regulating tubulin dynamics, thus is important for zebrafish oogenesis and embryogenesis.
Liu
Jianxiong
J
Department of Biology, The University of Memphis, 201 Life Science Building, Memphis, TN 38152-3560, USA.
Lessman
Charles A
CA
D000818:Animals; D004625:Embryo, Nonmammalian / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism*; D010053:Ovary / Q000737:chemistry / Q000196:embryology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D014404:Tubulin; D029961:Zebrafish Proteins