Effect of beta-adrenoceptor blockers on growth of neurites in spinal ganglia in organotypic tissue culture.
We studied the effects of norepinephrine and beta1-adrenoblockers atenolol and metoprolol on the growth of neuritis in organotypic tissue culture of spinal ganglia from 10-12-day chicken embryos. Atenolol (10(-4) M) accelerated the growth of neurites in spinal ganglia by 45%, while metoprolol inhibited the growth of the explants almost in all examined concentrations (10(-10)-10(-4) M). Thus, beta1-adrenoblockers can produce opposite effects on neurite growth. Both atenolol and metoprolol abolished the neurite-stimulating effect of norepinephrine, probably, because this effect is mediated via beta1-adrenoreceptors of spinal ganglia.
Penniyainen
V A
VA
Laboratory of Excitable Membranes, I P Pavlov Institute of Physiology, Russian Academy of Sciences, St Petersburg, Russia.
Lopatina
E V
EV
Tsyrlin
V A
VA
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D001262:Atenolol / Q000494:pharmacology; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D005727:Ganglia, Spinal / Q000187:drug effects*; D008790:Metoprolol / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects*; D046509:Tissue Culture Techniques
D000319:Adrenergic beta-Antagonists; D001262:Atenolol; D008790:Metoprolol