title: Effects of methanol extract of Alisma orientale rhizome and its major component, alisol B 23-acetate, on hepatic drug metabolizing enzymes in rats treated with bromobenzene.
abstract: In the course of screening for hepatoprotective agents from natural products, the effects of the methanol extract (ME) of the rhizome of Alisma orientale (Alismataceae) and its major component, alisol B 23-acetate (ALB) on hepatic lipid peroxidation and drug-metabolizing enzymes were evaluated in rats intoxicated with bromobenzene (BB). Pretreatment with ME and ALB had no effect on hepatic antioxidant enzymes such as glutathione reductase and a-glutamylcysteine synthetase. ME and ALB had also no effect on the reduction in glutathione content caused by BB. In contrast, ME recovered the BB-induced decrease in epoxide hydrolase and glutathione S-transferase, enzymes that remove toxic epoxides. ME also attenuated the BB-induced increase in aminopyrine N-demethylase and aniline hydroxylase, enzymes that produce toxic intermediates. This effect was greater than that seen with ascorbic acid, which was used as a positive control. ALB had similar effects on the activities of antioxidant enzymes to ME, and may be partly responsible for the effects of ME.
authors.affiliation: Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Suncheon, Jeonnam 540-742, Korea.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Hur
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Park
mesh_terms: D034902:Alisma / Q000737:chemistry*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001969:Bromobenzenes / Q000633:toxicity*; D002783:Cholestenones / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification*; D004851:Epoxide Hydrolases / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001969:Bromobenzenes; D002783:Cholestenones; D004224:Diterpenes; D010936:Plant Extracts; C526957:alisol B 23-acetate; C526955:hemsleyanine C; C032036:bromobenzene; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases; D005978:Glutathione; D001205:Ascorbic Acid