title: Cytokine regulation of intercellular adhesion molecule-1 expression on trophoblasts in preeclampsia.
abstract: In normal pregnancy, trophoblast (TR) invasion plays a crucial role in remodeling the spiral arteries to develop uteroplacental circulation. Disruption of this invasion is associated with deficient uteroplacental circulation, which can lead to the development of preeclampsia (PE) through abnormal expression of adhesion molecules in the placenta and high serum causative factors such as cytokines. We aimed to evaluate whether serum factors in PE influence intercellular adhesion molecule-1 (ICAM-1) expression of TRs. ICAM-1 expression of TRs was measured using flow cytometry and immunohistochemistry was performed to examine the localization of tumor necrosis factor-alpha (TNFalpha) and ICAM-1 in placentas derived from women with normal pregnancies and women with PE. Sera from PE patients significantly increased ICAM-1 expression on TRs compared to sera from normal pregnant women; this increase was blocked with an antibody to TNFalpha. TNFalpha also enhanced ICAM-1 expression on TRs through nuclear factor-kappaB activation. We conclude that ICAM-1 expressed on TRs is involved in PE pathogenesis and is regulated by cytokines.
authors.affiliation: Department of Obstetrics and Gynecology, Ehime University School of Medicine, Toon, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Abe
authors.forename: Keiichi
authors.initials: K
authors.lastname: Matsubara
authors.forename: Keizo
authors.initials: K
authors.lastname: Oka
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Kusanagi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ito
mesh_terms: D000328:Adult; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1