title: Mechanisms of intron gain and loss in Cryptococcus.
abstract: BACKGROUND
  Genome comparisons across deep phylogenetic divergences have revealed that spliceosomal intron gain and loss are common evolutionary events. However, because of the deep divergences involved in these comparisons, little is understood about how these changes occur, particularly in the case of intron gain. To ascertain mechanisms of intron gain and loss, we compared five relatively closely related genomes from the yeast Cryptococcus.
  
  
  RESULTS
  We observe a predominance of intron loss over gain and identify a relatively slow intron loss rate in Cryptococcus. Some genes preferentially lose introns and a large proportion of intron losses occur in the middle of genes (so called internal intron loss). Finally, we identify a gene that displays a differential number of introns in a repetitive DNA region.
  
  
  CONCLUSIONS
  Based the observed patterns of intron loss and gain, population resequencing and population genetic analysis, it appears that recombination causes the widely observed but poorly understood phenomenon of internal intron loss and that DNA repeat expansion can create new introns in a population.
authors.affiliation: Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, CA 94720, USA. sharpton@berkeley.edu
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Sharpton
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Neafsey
authors.forename: James E
authors.initials: JE
authors.lastname: Galagan
authors.forename: John W
authors.initials: JW
authors.lastname: Taylor
mesh_terms: D001483:Base Sequence; D003454:Cryptococcus / Q000235:genetics*; D042622:DNA Repeat Expansion; D019143:Evolution, Molecular; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D016415:Sequence Alignment