title: RNase domains determine the functional difference between IRE1alpha and IRE1beta.
abstract: Endoplasmic reticulum (ER) stress is associated with the functional disorder of the ER. During conditions of ER stress, cells induce at least two responses to maintain ER function: transcriptional upregulation of ER quality control genes, and translational attenuation of protein synthesis. Induction of ER quality control proteins is mediated by IRE1alpha, which activates the transcription factor XBP1 via an unconventional splicing event, while a partial translational attenuation is mediated by IRE1beta. Here, we show by both in vivo and in vitro analyses that the RNase domain of IRE1 determines the functional specificities of each of these isoforms.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Imagawa
authors.forename: Akira
authors.initials: A
authors.lastname: Hosoda
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Sasaka
authors.forename: Akio
authors.initials: A
authors.lastname: Tsuru
authors.forename: Kenji
authors.initials: K
authors.lastname: Kohno
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D004722:Endoribonucleases / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D009690:Nucleic Acid Conformation; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012326:RNA Splicing / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D000071701:Regulatory Factor X Transcription Factors; D012260:Ribonucleases / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1
chemical_list: D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605543:XBP1 protein, human; C403982:ERN2 protein, human; C528229:ERN1 protein, human; D017346:Protein Serine-Threonine Kinases; D004722:Endoribonucleases; D012260:Ribonucleases