[Investigation on cellular uptake and cytotoxicity of plasmid DNA-chitosan nanoparticles].
Two kinds of chitosan of different molecular weight (50 kDa and 400 kDa) were employed to form nanoparticles with 32P-labeled plasmid DNA at different N/P ratios by complex coacervation method. The characteristics of chitosan gene nanoparticles (CGN) were measured. The cellular uptake of DNA nanoparticles was evaluated by A10 and K562 cells. The in vitro cytotoxicity of DNA nanoparticles was determined by the MTT assays. Cellular uptake of the DNA nanoparticles increased with increasing chitosan molecular weight and N/P ratio. It also correlated with the zeta potential of the DNA nanoparticles. Chitosan-DNA nanoparticles were much less cytotoxic when compared with Lipofectamine 2000-DNA nanoparticles.
Zhang
Hailing
H
Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China.
Wang
Qin
Q
Song
Liping
L
Yue
Jingyin
J
Leng
Xigang
X
D001704:Biopolymers / Q000737:chemistry / Q000633:toxicity*; D048271:Chitosan / Q000737:chemistry / Q000633:toxicity*; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000737:chemistry / Q000633:toxicity*; D018014:Gene Transfer Techniques; D006801:Humans; D020014:K562 Cells; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D010957:Plasmids / Q000737:chemistry / Q000633:toxicity*
D001704:Biopolymers; D004247:DNA; D048271:Chitosan