Phenotypic and genetic characterization of Edwardsiella tarda isolated from pond sediments.
The extent of genotypic and phenotypic diversity of Edwardsiella tarda isolated from pond sediment was assessed by SDS-PAGE, Plasmid Profiling and ERIC-PCR. SDS-PAGE of whole cell protein extracts reveals 20-23 discrete bands with molecular wt of 14-110 kDa. Several bands with molecular weight range of 38-83 kDa were present in all the isolates. Numerical analysis of protein electrophoregram delineated the isolates into four clusters. Two different types of plasmids having molecular mass of 23 kDa and 29 kDa were obtained by plasmid profiling. About 51% of the isolates carried both the plasmids. ERIC-PCR generates 3-7 bands with molecular mass of 14-1013 bp. Numerical analysis differentiated the ERIC pattern into 5 clusters at 60% similarly level. It was concluded that out of three methods ERIC-PCR was found to be more sensitive for intraspecific typing of E. tarda and can be used as a potential tool for epidemiological studies in the future.
Maiti
N K
NK
Microbiology section, Central Institute of Freshwater Aquaculture, Kaushalyaganga, Bhubaneswar 751002, Orissa, India. maitink@yahoo.co.in
Mandal
Arunika
A
Mohanty
S
S
Mandal
R N
RN
D015373:Bacterial Typing Techniques; D020609:Edwardsiella tarda / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005618:Fresh Water / Q000382:microbiology*; D014644:Genetic Variation; D019015:Geologic Sediments / Q000382:microbiology; D010641:Phenotype; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D040081:Protein Array Analysis; D014871:Water Microbiology