Focusing on RISC assembly in mammalian cells.
RISC (RNA-induced silencing complex) is a central protein complex in RNAi, into which a siRNA strand is assembled to become effective in gene silencing. By using an in vitro RNAi reaction based on Drosophila embryo extract, an asymmetric model was recently proposed for RISC assembly of siRNA strands, suggesting that the strand that is more loosely paired at its 5' end is selectively assembled into RISC and results in target gene silencing. However, in the present study, we were unable to establish such a correlation in cell-based RNAi assays, as well as in large-scale RNAi data analyses. This suggests that the thermodynamic stability of siRNA is not a major determinant of gene silencing in mammalian cells. Further studies on fork siRNAs showed that mismatch at the 5' end of the siRNA sense strand decreased RISC assembly of the antisense strand, but surprisingly did not increase RISC assembly of the sense strand. More interestingly, measurements of melting temperature showed that the terminal stability of fork siRNAs correlated with the positions of the mismatches, but not gene silencing efficacy. In summary, our data demonstrate that there is no definite correlation between siRNA stability and gene silencing in mammalian cells, which suggests that instead of thermodynamic stability, other features of the siRNA duplex contribute to RISC assembly in RNAi.
Hong
Junmei
J
Institute of Molecular Medicine, Peking University, 100871 Beijing, PR China.
Wei
Na
N
Chalk
Alistair
A
Wang
Jue
J
Song
Yutong
Y
Yi
Fan
F
Qiao
Ren-Ping
RP
Sonnhammer
Erik L L
EL
Wahlestedt
Claes
C
Liang
Zicai
Z
Du
Quan
Q
D000818:Animals; D002478:Cells, Cultured; D003198:Computer Simulation; D004331:Drosophila melanogaster; D008956:Models, Chemical; D008957:Models, Genetic; D008958:Models, Molecular; D034622:RNA Interference / Q000502:physiology*; D034743:RNA-Induced Silencing Complex / Q000737:chemistry* / Q000235:genetics* / Q000648:ultrastructure
D034743:RNA-Induced Silencing Complex