[Role of Rac1 activation in migration and invasion of colorectal cancer cell line SW480].
OBJECTIVE
To study the role of activation of Rac1 in colorectal cancer cell migration and invasion.


METHODS
Rac1 L61 plasmid and control plasmid were transfected into colorectal cancer cell line SW480 cells. Pull down assay by Western blotting was carried out to measure the amount of activited Rac1, and transwell permeable supports were used to assess the migration and invasion of SW480 cells with different activitivity of Rac1.


RESULTS
The transfection ratio of SW480 cells was more than 80%. Pull down assay showed that the activited Rac1 was significantly higher in the SW480 cells transfected with Rac1 L61 plasmid than that in the control, and the amount of migrating and invasing SW480 cells transfected with Rac1 L61 plasmid in the Transwell permeable supports were significantly more than those in controls (migrating cell numbers: 43 +/- 9 vs. 22 +/- 5, P < 0.01; invasing cell numbers: 73 +/- 13 vs. 38 +/- 1, P < 0.01).


CONCLUSIONS
The activation of Rac1 plays an important role in the migration and invasion of colorectal cancer cells.
Nan
Qing-Zhen
QZ
Institute of Digestive Diseases, Nanfang Hospital, South Medical University, Guangzhou 510515, China. nanqingzhen@126.com
Gao
Lei
L
Zhang
Zhen-Shu
ZS
D045744:Cell Line, Tumor; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004789:Enzyme Activation; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009361:Neoplasm Invasiveness; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D020830:rac1 GTP-Binding Protein