title: Purification and analysis of thrombospondin-1.
abstract: Thromboapondin 1 (TSP-1) is a trimeric matricellular protein that is expressed by many cells. It contains several different domains that allow it to participate in cell adhesion, cell migration, and cell signaling. Recently TSP-1 has been shown to activate transforming growth factor beta (TGF-beta) and to inhibit both angiogenesis and tumor growth. This unit contains protocols for the purification of TSP-1 from platelet-rich plasma and the purification of TSP-1 proteolytic fragments.
authors.affiliation: Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
authors.forename: Karen O
authors.initials: KO
authors.lastname: Yee
authors.forename: Mark
authors.initials: M
authors.lastname: Duquette
authors.forename: Anna
authors.initials: A
authors.lastname: Ludlow
authors.forename: Jack
authors.initials: J
authors.lastname: Lawler
mesh_terms: D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000737:chemistry*; D006801:Humans; D008722:Methods; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000737:chemistry; D019700:Thrombospondin 1 / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010446:Peptide Fragments; D019700:Thrombospondin 1; D010447:Peptide Hydrolases