In vitro analysis of endoplasmic-reticulum-to-golgi transport in mammalian cells.
A temperature-sensitive mutant of vesicular stomatitis G protein is used to follow the movement of that protein from the endoplasmic reticulum to transport vesicles to cis-Golgi and finally medial/trans-Golgi by assessing the maturation of two asparagine-linked oligosaccharides. These assays can be used to identify the factors that are required for and regulate protein trafficking through these compartments.
Allan
B B
BB
The Scripps Research Institute, La Jolla, California, USA.
Balch
W E
WE
D020823:ADP-Ribosylation Factor 1; D000818:Animals; D001216:Asparagine / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000821:virology; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism* / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D033921:Vesicular Transport Proteins; D018116:Vesiculovirus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D033921:Vesicular Transport Proteins; D014759:Viral Envelope Proteins; D001216:Asparagine; D020823:ADP-Ribosylation Factor 1