Ovine anti-rabies antibody production and evaluation.
In view of the disadvantages of human and equine rabies immunoglobulin still there is urgent needs for safe and cost-control anti-rabies immunoglobulins especially for person who have been severely exposed (categories III) to the virus. Our attempt to produce a less immunogenic and cheaper anti-rabies immunoglobulin affordable for those people living in developing countries, has been harnessed the ovine as a bioreactor instead the horse. The animals have been intramuscular immunized, and the plasma processed with 5% caprylic acid to yield IgG with purity of 95%. Moreover, antibody apparently indicated that the titer and neutralizing indexes were harmonized, especially at higher antibody dilution. The results showed that three immunized sheep were produced about 7000 IU of purified anti-rabies antibody. Sheep's IgG has low immunogenic effect than human and horse antibodies when injected into the mouse. Pure concentrated ovine antibody may serve as a possible alternative to currently available anti-rabies human or equine immunoglobulin.
Redwan
El-Rashdy M
el-RM
Protein Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt. redwan1961@yahoo.com
Fahmy
Ali
A
El Hanafy
Amr
A
Abd El-Baky
Nawal
N
Sallam
Sobhy M A
SM
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D002162:Camelus / Q000276:immunology; D002522:Chlorocebus aethiops; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D006736:Horses / Q000276:immunology; D006801:Humans; D007114:Immunization; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D011818:Rabies / Q000517:prevention & control*; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D034561:Sheep, Domestic / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011819:Rabies Vaccines