PAS-FTIR and FT-Raman qualitative characterization of sodium dodecyl sulfate interaction with an alternative stratum corneum model membrane.
The interaction of the surfactant sodium dodecyl sulfate with the stratum corneum (SC) of shed snake skin from Bothrops jararaca, used as a model membrane, was characterized qualitatively by FT-Raman and infrared photoacoustic (PAS-FTIR) spectroscopy, used as analytical tools. Surfactant solutions were 50.0 g x l(-1) and 2.34 g x l(-1) with treatment intervals of 4, 8 and 12 h. The employment of FT-Raman and PAS-FTIR indicated increased hydration of the SC with alteration of the tissue topography. The interaction of the SC with surfactant was increased by the tape-stripping process. The consequent exposure of the internal layers of the tissue intensified the effect of the anionic surfactant, indicating that this layer acted as an additional barrier.
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Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, Brazil. andrerb@usp.br
Lacerda
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AC
Kawano
Y
Y
Velasco
M V R
MV
Kaneko
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TM
D000818:Animals; D017837:Bothrops / Q000378:metabolism*; D066298:In Vitro Techniques; D007633:Keratins / Q000737:chemistry; D008566:Membranes / Q000737:chemistry; D012867:Skin / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013501:Surface-Active Agents / Q000737:chemistry*; D014732:Vibration
D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D007633:Keratins