title: FTIR microspectroscopic analysis of the effects of certain drugs on oxidative stress and brain protein structure.
abstract: This study reports the changes in lipids and proteins of different brain areas of nicotine, D+-amphetamine, and nicotine and D+-amphetamine treated rats by monitoring lipid peroxidation and protein beta-sheet formation using infrared microspectroscopy. Compared with the untreated brain samples, the peroxide level is relatively higher in the amphetamine-treated brain sections, both in the cortex and hippocampus area. However, this peroxide increase is attenuated when administering amphetamine plus nicotine. Analogous drug-dependent trends for protein beta-sheet content are observed, which suggests a connection between lipid oxidation involved in oxidative stress and beta-sheet protein structure generally present in neurodegenerative diseases. The above property of nicotine is of interest, in the sense that it might reduce the production of beta-amyloid proteins in Alzheimer's disease.
authors.affiliation: Instituto de Estructura de la Materia (CSIC), Serrano 121, 28006 Madrid, Spain. p.carmona@iem.cfmac.csic.es
authors.forename: P
authors.initials: P
authors.lastname: Carmona
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez-Casado
authors.forename: I
authors.initials: I
authors.lastname: Alvarez
authors.forename: E
authors.initials: E
authors.lastname: de Miguel
authors.forename: A
authors.initials: A
authors.lastname: Toledano
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000661:Amphetamine / Q000494:pharmacology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D005731:Ganglionic Stimulants / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009538:Nicotine / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D017433:Protein Structure, Secondary; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D016229:Amyloid beta-Peptides; D000697:Central Nervous System Stimulants; D005731:Ganglionic Stimulants; D009538:Nicotine; D000661:Amphetamine