title: Unique and recurrent mutations in the filaggrin gene in Singaporean Chinese patients with ichthyosis vulgaris.
abstract: Filaggrin is an abundant protein of the outer epidermis that is essential for terminal differentiation of keratinocytes and formation of an effective barrier against water loss and pathogen/allergen/irritant invasion. Recent investigations in Europe and Japan have revealed null mutations in the filaggrin gene (FLG) as the underlying cause of ichthyosis vulgaris (IV), a common skin disorder characterised by dry skin, palmar hyperlinearity and keratosis pilaris. Following the development of a strategy for the comprehensive analysis of FLG, we have identified five unique mutations and one recurrent mutation in Singaporean Chinese IV patients. Mutation 441delA is located in the profilaggrin S100 domain, whereas two additional frameshift mutations, 1249insG and 7945delA, occur in the first partial filaggrin repeat ("repeat 0") and in filaggrin repeat 7, respectively. Both nonsense mutations Q2147X and E2422X are found in filaggrin repeat 6, whereas R4307X was found on one of the longer size variant alleles of FLG, within duplicated repeat 10.2. Mutation E2422X, previously found in a single Dutch patient, was found in one Singaporean IV patient and at a low frequency in Asian population controls. Our study confirms the presence of population-specific as well as recurrent FLG mutations in Singapore.
authors.affiliation: Epithelial Genetics Group, Human Genetics Unit, Division of Pathology and Neuroscience, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK.
authors.forename: Huijia
authors.initials: H
authors.lastname: Chen
authors.forename: Jean C C
authors.initials: JC
authors.lastname: Ho
authors.forename: Aileen
authors.initials: A
authors.lastname: Sandilands
authors.forename: Yuin Chew
authors.initials: YC
authors.lastname: Chan
authors.forename: Yoke Chin
authors.initials: YC
authors.lastname: Giam
authors.forename: Alan T
authors.initials: AT
authors.lastname: Evans
authors.forename: E Birgitte
authors.initials: EB
authors.lastname: Lane
authors.forename: W H Irwin
authors.initials: WH
authors.lastname: McLean
mesh_terms: D044466:Asian People / Q000235:genetics; D000091344:Filaggrin Proteins; D006801:Humans; D016112:Ichthyosis Vulgaris / Q000235:genetics*; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000235:genetics*; D009154:Mutation; D012846:Singapore
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins