Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies.
A simple HPLC-UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C(18) column by isocratic elution with acetonitrile-water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine in Cordyceps were 0.28-14.15, 0.006-6.36, 0.01-0.14, 0.68-14.79 and 0.19-20.29 mg/g, respectively. Among the nucleosides studied, cordycepin was characteristically included in Cordyceps militaris (L.) Link. (CM), which is one of key Cordyceps allies, and might be a good marker for authenticating CM. The ratio of nucleosides to adenosine contents in Cordyceps seemed to be a useful marker for authentication and quality control of Cordyceps.
Ikeda
Rie
R
Division of Analytical Research for Pharmacoinformatics, Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Nishimura
Miho
M
Sun
Yen
Y
Wada
Mitsuhiro
M
Nakashima
Kenichiro
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry*; D009705:Nucleosides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D009705:Nucleosides