Isolation of rat hepatocyte plasma membrane sheets and plasma membrane domains.
This unit describes a method for isolation of plasma membrane sheets from rat liver. It also includes protocols for preparation of plasma membrane domains isolated from plasma membrane sheets and indirect immunofluorescence localization of marker proteins associated with plasma membrane sheets. The unit has been updated with assays for the marker enzymes alkaline phosphodiesterase I, 5' nucleotidase, and K+-stimulated.
Tuma
P L
PL
Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Hubbard
A L
AL
D009597:4-Nitrophenylphosphatase / Q000032:analysis; D015720:5'-Nucleotidase / Q000032:analysis; D000818:Animals; D015415:Biomarkers; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000201:enzymology*; D019084:Fluorescent Antibody Technique, Indirect; D022781:Hepatocytes / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D051381:Rats; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D008562:Membrane Glycoproteins; D009597:4-Nitrophenylphosphatase; D015720:5'-Nucleotidase; D010727:Phosphoric Diester Hydrolases; C524442:Enpp3 protein, rat; D011755:Pyrophosphatases