Polyunsaturated fatty acids production with a solid-state column reactor.
To investigate the potential production of polyunsaturated fatty acids (PUFAs), a solid-state column reactor of rice bran with Mortierella alpina was used. The optimal conditions for PUFAs production were rice bran supplementation with 3.75% (ww(-1)) nitrogen source at initial moisture content 57%, initial pH 6-7, aeration, and incubation at 20 degrees C for 5 days and then at 12 degrees C for 7 days. Each gram of substrate carbon yielded 127 mg of total PUFAs, 12 mg of eicosapentaenoic acid (EPA), 6 mg of arachidonic acid (AA), 5mg of alpha-linolenic acid (ALA), and 117 mg of linoleic acid (LA) after 12 days incubation. Aeration enhanced the productions of AA, EPA, and total PUFAs. Supplementation of the nitrogen source on the fourth day and then a shift to lower temperature on the fifth day increased EPA production.
Jang
Hung-Der
HD
Department of Food Science, Yuanpei University, Hsinchu 300, Taiwan.
Yang
Shang-Shyng
SS
D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D020094:Mortierella / Q000378:metabolism*; D009584:Nitrogen / Q000008:administration & dosage; D012275:Oryza / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D009584:Nitrogen