title: PACAP is transiently expressed in anterior pituitary gland of rats: in situ hybridization and cell immunoblot assay studies.
abstract: In this work the expression of PACAP (pituitary adenylate cyclase activating polypeptide) in rat anterior pituitary was demonstrated for the first time using in situ hybridization. The number of cells showing PACAP signal in intact male rats was negligible similarly to that of diestrous rats. In proestrous rats sacrificed at 10h there was a moderate increase in the expression and after a decrease at 16 h and 18 h, there was a transient peak at 20 h and then the number of labeled cells was declined again (22 h). In the cell immunoblot assay study it was observed that the number of PACAP blot forming (PACAP releasing) cells in an anterior pituitary cell culture changed according to a similar pattern as the number of PACAP expressing cells. The number of blots was also the highest when the animals were sacrificed in the evening of proestrus at 20h. The results obtained by in situ hybridization and cell immunoblot assay well correlate with each other. The above-mentioned results support our hypothesis that the enhanced expression and secretion of PACAP in the pituitary gland may be involved in ceasing the LH surge.
authors.affiliation: Department of Human Morphology and Developmental Biology, Faculty of Medicine, Semmelweis University, Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Heinzlmann
authors.forename: Eszter
authors.initials: E
authors.lastname: Kirilly
authors.forename: Kinga
authors.initials: K
authors.lastname: Meltzer
authors.forename: Eniko
authors.initials: E
authors.lastname: Szabó
authors.forename: Akemichi
authors.initials: A
authors.lastname: Baba
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Katalin
authors.initials: K
authors.lastname: Köves
mesh_terms: D000818:Animals; D004971:Estrus / Q000502:physiology; D005260:Female; D015151:Immunoblotting; D017403:In Situ Hybridization; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D007986:Luteinizing Hormone