title: One-generation reproductive toxicity study of dietary 17beta-estradiol (E2; CAS No. 50-28-2) in CD-1 (Swiss) mice.
abstract: There is no information on reproductive/developmental effects in mice from dietary estrogen. Therefore, 10 adult CD-1 mice/sex/group were administered dietary 17beta-estradiol (E2) at 0, 0.005, 0.05, 0.5, 2.5, 5, 10, and 50 ppm for 2-week prebreed, mating, gestation, lactation. F1 weanlings (3/sex/litter) were necropsied and 2/sex/litter were retained, with exposure, until vaginal patency (VP) or preputial separation (PPS) and then necropsied. Results included complete infertility at 2.5-50 ppm with normal mating indices. At 0.5 ppm (and above), F0 adult female uterus plus cervix plus vagina weights (UCVW) were increased. At 0.5 ppm: prolonged gestational length; increased F1 stillbirth index; reduced live birth index and litter size; decreased testes and epididymides weights at weaning; unaffected AGD on pnd 0 and 21; delayed PPS; increased undescended testes; unaffected prostate weight; accelerated VP; enlarged vaginas; fluid-filled uteri. At 0.05 ppm: no F0 reproductive effects, increased F1 weanling UCVW; delayed PPS. The NOEL was 0.005 ppm ( approximately 1 microg/kg/day).
authors.affiliation: Health Sciences Unit, RTI International, Research Triangle Park, NC 27709-2194, United States. rwt@rti.org
authors.forename: Rochelle W
authors.initials: RW
authors.lastname: Tyl
authors.forename: Christina B
authors.initials: CB
authors.lastname: Myers
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Marr
authors.forename: Nora P
authors.initials: NP
authors.lastname: Castillo
authors.forename: M Michael
authors.initials: MM
authors.lastname: Veselica
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Joiner
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Dimond
authors.forename: John P
authors.initials: JP
authors.lastname: Van Miller
authors.forename: Gisela D
authors.initials: GD
authors.lastname: Stropp
authors.forename: John M
authors.initials: JM
authors.lastname: Waechter
authors.forename: Steven G
authors.initials: SG
authors.lastname: Hentges
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects*
chemical_list: D004958:Estradiol