[Genetic polymorphism in cell cycle control genes and susceptibility of chromosomal damage in vinyl chloride monomer exposed workers.].
OBJECTIVE
To explore the relationship between genetic polymorphism of P53, P21, CCND1 and susceptibility of chromosomal damage induced by vinyl chloride monomer (VCM).


METHODS
183 workers occupationally exposed to VCM were involved in our study. Cytokinesis-block micronucleus (CB-MN) assay was used to detect chromosome damage in peripheral lymphocyte. PCR-RFLP technique was applied to detect polymorphisms in P53 gene (exon4, intron3 and intron6), P21 gene (exon2 and exon3) and CCND1 (exon4).


RESULTS
The risk of chromosomal damage for VCM-exposed workers with more than 30 yr was 1.2202 (95% CI: 1.0580 approximately 1.4072, P = 0.0062) compared with the younger workers, and the risk of female workers was 1.1491 (95% CI: 0.9841 approximately 1.3416, P = 0.0772) compared with male workers. The MN frequency in subjects with P53 intron6 mutant homozygous and heterozygous was higher than their wild-type homozygous counterparts (OR = 1.3032, 95% CI: 1.1285 approximately 1.6405, P = 0.0285). P53 exon4, intron3 and intron6 haplotype pairs of BBB/AAA and BAB/AAA were associated with the increased frequencies of micronucleus.


CONCLUSIONS
Among VCM-exposed workers, more than 30ys, female, carrying P53 intron6 mutated allele and BBB/AAA and BAB/AAA haplotype pairs have higher risk of chromosomal damage.
Qiu
Yu-Lan
YL
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Wang
Wei
W
Sun
Pin
P
Liu
Jing
J
Li
Jun
J
Chai
Shang-Jian
SJ
Xia
Zhao-Lin
ZL
D059447:Cell Cycle Checkpoints; D006801:Humans; D015162:Micronucleus Tests; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D014752:Vinyl Chloride
D014752:Vinyl Chloride