title: Genomovar status, virulence markers and genotyping of Burkholderia cepacia complex strains isolated from Brazilian cystic fibrosis patients.
abstract: Burkholderia cepacia complex isolates obtained by microbiological culture of respiratory samples from Brazilian CF patients were studied by recA based PCR, screened by specific PCR for virulence markers and genotyped by RAPD. Forty-one isolates of B. cepacia complex were identified by culture and confirmation of identity and genomovar determination obtained in 32 isolates, with predominance of B. cenocepacia (53.1%). Virulence markers were not consistently found among isolates. Genotyping did not identify identical patterns among different patients. B. cenocepacia was the most prevalent B. cepacia complex member among our patients, and cross-infection does not seem to occur among them.
authors.affiliation: Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil.
authors.forename: Kátia Maia
authors.initials: KM
authors.lastname: Martins
authors.affiliation: Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil.
authors.forename: Giuliana F
authors.initials: GF
authors.lastname: Fongaro
authors.affiliation: Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil.
authors.forename: André B
authors.initials: AB
authors.lastname: Dutra Rodrigues
authors.affiliation: Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Tateno
authors.affiliation: Bacteriology Section, Adolfo Lutz Institute, São Paulo, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Azzuz-Chernishev
authors.affiliation: Bacteriology Section, Adolfo Lutz Institute, São Paulo, Brazil.
authors.forename: Doroti de
authors.initials: D
authors.lastname: Oliveira-Garcia
authors.affiliation: Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil.
authors.forename: Joaquim C
authors.initials: JC
authors.lastname: Rodrigues
authors.affiliation: Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil; Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil. Electronic address: vicres@usp.br.
authors.forename: Luiz Vicente F
authors.initials: LVF
authors.lastname: da Silva Filho
mesh_terms: D001938:Brazil / Q000453:epidemiology; D019121:Burkholderia Infections / Q000453:epidemiology / Q000235:genetics*; D042602:Burkholderia cepacia complex / Q000235:genetics* / Q000302:isolation & purification*; D015331:Cohort Studies; D003550:Cystic Fibrosis / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D015995:Prevalence; D011938:Rec A Recombinases / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D011938:Rec A Recombinases