Lentivirus transduction of bone marrow hemopoietic precursor cells with Lin-c-kit+ phenotype ex vivo using a genetic construct containing green fluorescent protein gene.
We developed a technology of labeling bone marrow precursor cells with the Lin-c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies.
Radyukhina
N V
NV
Department of Cell Biology, Institute of Experimental Cardiology, Cardiology Research-and-Production Complex, Federal Agency for Health Care and Social Development, Moscow. cardiocell@cardio.ru
Rutkevich
P N
PN
Aref'yeva
T I
TI
Gurskaya
T Kh
TKh
Rybalkin
I N
IN
Shevelyov
A Ya
AY
Slinkin
M A
MA
Vlasik
T N
TN
Il'yinskaya
O P
OP
Tararak
E M
EM
D000818:Animals; D005260:Female; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000821:virology*; D016086:Lentivirus / Q000502:physiology*; D008297:Male; D051379:Mice; D014161:Transduction, Genetic / Q000379:methods*
D049452:Green Fluorescent Proteins