The influence of carbohydrate structures present in common allergen sources on specific IgE results.
BACKGROUND
Cross-reactive carbohydrate determinants (CCD) are well known interferants in specific IgE assays (sIgE). Glyco-epitopes are not restricted to CCD and extracts used to prepare in vitro tests contain many other glycoproteins able to bind glycan-specific IgE. The overall amounts of IgE-bindable glycan structures in allergen sources are unknown.


OBJECTIVE
We aimed at quantifying the influence of N-glycan structures on IgE reactivity to commonly tested allergen sources.


METHODS
IgE reactivity to 51 allergen extracts, one purified natural allergen and 10 recombinant allergens was measured on Phadia UniCAP system using 2 sera demonstrating significant levels of glycan-related IgE reactivity. Immobilized bromelain and horseradish peroxidase (HRP) were used to capture N-glycan-specific IgE from these sera. Residual IgE reactivity was measured for 42 allergen sources and 4 recombinant/purified allergens.


RESULTS
An obviously excessive number of positive CAP-results were obtained with both sera, especially for plant-based allergen sources. Capture of glycan-specific IgE led to a decrease of serum IgE ractivity, variable among allergen sources and between sera. Among others, peanut results were proven largely interfered by the presence of glycan-specific IgE. Unexpectedly some allergen sources showed a slight influence of glycan-related reactivity, such as cockroach, mosquito, mussel, shrimp and domestic mites.


CONCLUSIONS
In patients sensitized to pollens or to Hymenoptera venoms sIgE results should be interpreted with caution. One cannot substract the result of a glyco-reporter test (bromelain and/or HRP) in order to compute glycan-free slgE results for common allergen sources like peanuts. As long as the demonstration of a significant role for glycan structures in clinical allergic reactions is lacking, a simple pre-treatment able to discard glycan-specific IgE from serum would be useful to improve accuracy of in vitro diagnostic tests.
Malandain
H
H
Clinical Biochemistry Laboratory, Chubert Hospital, 56000 Vannes, France. henri.malandain@ch-bretagne-atlantique.fr
Giroux
F
F
Cano
Y
Y
D000485:Allergens / Q000737:chemistry* / Q000276:immunology*; D000918:Antibody Specificity; D002241:Carbohydrates / Q000737:chemistry* / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000175:diagnosis / Q000517:prevention & control; D007118:Immunoassay / Q000379:methods; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D013329:Structure-Activity Relationship
D000485:Allergens; D002241:Carbohydrates; D000939:Epitopes; D007073:Immunoglobulin E