title: Expression, purification, and crystallization of neuro- and cytoglobin.
abstract: Neuroglobin and cytoglobin, members of the globin family, are present in vertebrate cells at very low concentrations. As the function of both proteins is still a matter of debate, it is very important to be able to produce and purify these proteins, and in general all members of the globin family, to homogeneity. For this purpose, this chapter describes the expression of neuro- and cytoglobin by E. coli and its preparative purification. These proteins are then used in crystallization experiments. Also an analytical purification strategy is discussed in detail.
authors.affiliation: Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dewilde
authors.forename: Kirsten
authors.initials: K
authors.lastname: Mees
authors.forename: Laurent
authors.initials: L
authors.lastname: Kiger
authors.forename: Christophe
authors.initials: C
authors.lastname: Lechauve
authors.forename: Michael C
authors.initials: MC
authors.lastname: Marden
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pesce
authors.forename: Martino
authors.initials: M
authors.lastname: Bolognesi
authors.forename: Luc
authors.initials: L
authors.lastname: Moens
mesh_terms: D000180:Acrylic Resins; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003460:Crystallization; D000078842:Cytoglobin; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D005914:Globins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D000078787:Neuroglobin; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012685:Sepharose
chemical_list: D000180:Acrylic Resins; C000627265:CYGB protein, human; D000078842:Cytoglobin; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D011994:Recombinant Proteins; C023049:sephacryl S 200; D005914:Globins; D012685:Sepharose; C049225:Superose 12