title: Intravenous infusion of PAF affects ovulation, fertilization and preimplantation embryonic development in NZB x NZW F1 hybrid mice.
abstract: Platelet Activating Factor (PAF) is a bioactive phospholipid, which exhibits a variety of biological activities and plays a significant role in all aspects of reproduction. In this work, a single intravenous injection of various concentrations of PAF shortly after Human Chorionic Gonadotropin (HCG) administration as well as 24 and 48 h before HCG administration was studied in NZB x NZW F1 hybrid mice. Optimum results were observed when PAF was injected just after the administration of HCG. In this protocol, the concentrations of PAF exhibited bell-shaped response to every stage of development. Any concentration of PAF between 5.5 x 10(-11) and 5.5 x 10(-15)g/g b.w., caused an improved ovulation rate, an increased fertilization rate, an increased rate of cell cycle and an enhanced hatching blastocyst rate (P<0.05 for all stages). Injection of lyso-PAF had no effect in any stage. Our data show that the effect of PAF on early stages of embryo development in vitro is dependent on its way of administration, on the concentrations used as well as on the time PAF is injected.
authors.affiliation: 1st Department of Obstetrics and Gynecology, Athens University Medical School, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Sakellariou
authors.forename: Peter
authors.initials: P
authors.lastname: Drakakis
authors.forename: Smaragdi
authors.initials: S
authors.lastname: Antonopoulou
authors.forename: Elli
authors.initials: E
authors.lastname: Anagnostou
authors.forename: Dimitris
authors.initials: D
authors.lastname: Loutradis
authors.forename: Theoxaris
authors.initials: T
authors.lastname: Patargias
mesh_terms: D000818:Animals; D047108:Embryonic Development / Q000187:drug effects*; D005306:Fertilization / Q000187:drug effects*; D006824:Hybridization, Genetic; D007262:Infusions, Intravenous; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D010060:Ovulation / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D013480:Superovulation
chemical_list: C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor