Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein.
BACKGROUND
The p49/STRAP (or SRFBP1) protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart.


RESULTS
In the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49/STRAP in multiple screenings using the yeast two-hybrid system. The p49/STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49/STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49/STRAP altered the intracellular level of NAD, and reduced the NAD/NADH ratio. Overexpression of p49/STRAP also induced the deacetylation of serum response factor.


CONCLUSIONS
These data suggest that p49/STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.
Zhang
Xiaomin
X
From the Donald W. Reynolds Department of Geriatrics, The University of Arkansas for Medical Sciences and Geriatric Research, Education, and Clinical Center, Little Rock, AR, USA. zhangxiaomin@uams.edu
Azhar
Gohar
G
Helms
Scott
S
Zhong
Ying
Y
Wei
Jeanne Y
JY
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D042967:Electron Transport Complex I / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D032383:Myocytes, Cardiac / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology*; D041681:NIH 3T3 Cells; D011485:Protein Binding; D021122:Protein Subunits / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology; D051381:Rats; D014018:Tissue Distribution; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology
C529063:NDUFAB1 protein, human; D021122:Protein Subunits; C496545:SRFBP1 protein, human; D014157:Transcription Factors; D009243:NAD; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I