title: The effects of panduratin A isolated from Kaempferia pandurata on the expression of matrix metalloproteinase-1 and type-1 procollagen in human skin fibroblasts.
abstract: Exposure of ultraviolet (UV) light on the skin induces photoaging associated with up-regulated matrix metalloproteinase (MMP) activities and decreased collagen synthesis. We investigated the effects of panduratin A isolated from Kaempferia pandurata Roxb. on the expression of matrix metalloproteinase-1 (MMP-1) and type-1 procollagen in UV-irradiated human skin fibroblasts. Cultured human fibroblasts were irradiated with UV (20 mJ/cm (2)) and panduratin A was added into the medium of the fibroblast culture. The expressions of MMP-1 and type-1 procollagen levels were measured using Western blot analysis and RT-RCR. Panduratin A in the range of 0.001 - 0.1 microM significantly reduced the expression of MMP-1 and induced the expression of type-1 procollagen at the protein and mRNA gene levels. Panduratin A showed stronger activity than epigallocatechin 3- O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can be a potential candidate for the prevention and treatment of skin aging brought about by UV.
authors.affiliation: Department of Biomaterials Science and Engineering, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul, Korea.
authors.forename: Jae-Seok
authors.initials: JS
authors.lastname: Shim
authors.forename: Yi-Young
authors.initials: YY
authors.lastname: Kwon
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Hwang
mesh_terms: D002460:Cell Line; D047188:Chalcones / Q000302:isolation & purification / Q000494:pharmacology*; D024042:Collagen Type I / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D011347:Procollagen / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects*; D014466:Ultraviolet Rays; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D047188:Chalcones; D024042:Collagen Type I; D011347:Procollagen; C482884:panduratin A; D020781:Matrix Metalloproteinase 1