Optimal method of collection of first-void urine for diagnosis of Chlamydia trachomatis infection in men.
First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup by quantitative PCR (32,533 versus 5,271 plasmids/ml; P < 0.0001). Consequently, the use of FirstBurst to collect a urine sample improved the sensitivity of a rapid test for Chlamydia over testing of samples collected with a urine cup (82 versus 47% sensitivity using PCR as a reference; P < 0.0015).
Wisniewski
Craig A
CA
Department of Haematology, University of Cambridge, EABC Site, Long Road, Cambridge CB2 2PT, United Kingdom.
White
John A
JA
Michel
Claude-Edouard C
CE
Mahilum-Tapay
Lourdes
L
Magbanua
Jose Paolo V
JP
Nadala
Elpidio Cesar B
EC
Barber
Penelope J
PJ
Goh
Beng T
BT
Lee
Helen H
HH
D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods*; D014556:Urine / Q000382:microbiology*