Novel SNPs of the mtDNA ND5 gene and their associations with several growth traits in the Nanyang cattle breed.
Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six breeds were 0.041-0.167. Polymorphism of the ND5 gene was shown to be associated with growth in the Nanyang breed. Individuals with haplotype B had greater hucklebone width than those with haplotype A when 6 months old (P < 0.01), as well as better body height, body length, body weight, and average daily gain at 6 months (P < 0.05). Therefore, haplotype B of the ND5 gene was likely to have a positive effect on growth traits at 6 months in the Nanyang breed.
Zhang
Bao
B
College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
Chen
Hong
H
Hua
Liushuai
L
Zhang
Chunlei
C
Kang
Xiangtao
X
Wang
Xinzhuang
X
Pan
Chuanying
C
Lan
Xianyong
X
Lei
Chuzhao
C
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development*; D004272:DNA, Mitochondrial / Q000235:genetics*; D006239:Haplotypes; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics
D004272:DNA, Mitochondrial; D021122:Protein Subunits; D009245:NADH Dehydrogenase