title: Strongyloides venezuelensis: the antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis.
abstract: The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of L3 were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite.
authors.affiliation: Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz s/n. CEP. 13083-970, Caixa Postal 6109, Campinas, SP, Brazil. ermachado@hotmail.com <ermachado@hotmail.com>
authors.forename: Eleuza Rodrigues
authors.initials: ER
authors.lastname: Machado
authors.forename: Lúcia Helena
authors.initials: LH
authors.lastname: Faccioli
authors.forename: Julia Maria
authors.initials: JM
authors.lastname: Costa-Cruz
authors.forename: Elaine Vicente
authors.initials: EV
authors.lastname: Lourenço
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Roque-Barreira
authors.forename: Maria do Rosário de Fátima
authors.initials: Mdo R
authors.lastname: Gonçalves-Pires
authors.forename: Marlene Tiduko
authors.initials: MT
authors.lastname: Ueta
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007814:Larva / Q000145:classification / Q000276:immunology; D051381:Rats; D012377:Rodentia; D016205:Sigmodontinae; D013321:Strongyloides / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G