Protein production and purification.
In selecting a method to produce a recombinant protein, a researcher is faced with a bewildering array of choices as to where to start. To facilitate decision-making, we describe a consensus 'what to try first' strategy based on our collective analysis of the expression and purification of over 10,000 different proteins. This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Karolinska Institutet, Schéeles väg 2, 171 77 Stockholm, Sweden.
Gräslund
Susanne
S
Nordlund
Pär
P
Weigelt
Johan
J
Hallberg
B Martin
BM
Bray
James
J
Gileadi
Opher
O
Knapp
Stefan
S
Oppermann
Udo
U
Arrowsmith
Cheryl
C
Hui
Raymond
R
Ming
Jinrong
J
dhe-Paganon
Sirano
S
Park
Hee-won
HW
Savchenko
Alexei
A
Yee
Adelinda
A
Edwards
Aled
A
Vincentelli
Renaud
R
Cambillau
Christian
C
Kim
Rosalind
R
Kim
Sung-Hou
SH
Rao
Zihe
Z
Shi
Yunyu
Y
Terwilliger
Thomas C
TC
Kim
Chang-Yub
CY
Hung
Li-Wei
LW
Waldo
Geoffrey S
GS
Peleg
Yoav
Y
Albeck
Shira
S
Unger
Tamar
T
Dym
Orly
O
Prilusky
Jaime
J
Sussman
Joel L
JL
Stevens
Ray C
RC
Lesley
Scott A
SA
Wilson
Ian A
IA
Joachimiak
Andrzej
A
Collart
Frank
F
Dementieva
Irina
I
Donnelly
Mark I
MI
Eschenfeldt
William H
WH
Kim
Youngchang
Y
Stols
Lucy
L
Wu
Ruying
R
Zhou
Min
M
Burley
Stephen K
SK
Emtage
J Spencer
JS
Sauder
J Michael
JM
Thompson
Devon
D
Bain
Kevin
K
Luz
John
J
Gheyi
Tarun
T
Zhang
Fred
F
Atwell
Shane
S
Almo
Steven C
SC
Bonanno
Jeffrey B
JB
Fiser
Andras
A
Swaminathan
Sivasubramanian
S
Studier
F William
FW
Chance
Mark R
MR
Sali
Andrej
A
Acton
Thomas B
TB
Xiao
Rong
R
Zhao
Li
L
Ma
Li Chung
LC
Hunt
John F
JF
Tong
Liang
L
Cunningham
Kellie
K
Inouye
Masayori
M
Anderson
Stephen
S
Janjua
Heleema
H
Shastry
Ritu
R
Ho
Chi Kent
CK
Wang
Dongyan
D
Wang
Huang
H
Jiang
Mei
M
Montelione
Gaetano T
GT
Stuart
David I
DI
Owens
Raymond J
RJ
Daenke
Susan
S
Schütz
Anja
A
Heinemann
Udo
U
Yokoyama
Shigeyuki
S
Büssow
Konrad
K
Gunsalus
Kristin C
KC
D005591:Chemical Fractionation / Q000379:methods*; D002627:Chemistry, Physical / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D011994:Recombinant Proteins / Q000302:isolation & purification* / Q000378:metabolism*
D011994:Recombinant Proteins