Interleukin 8 exhibits a pro-mitogenic and pro-survival role in radiation induced genomically unstable cells.
Radiation induced genomic instability can be perpetuated over time by the transmission of soluble factors. This can occur via cell-to-cell gap junction communication or the secretion/shedding of soluble factors. We have investigated whether our radiation induced chromosomally unstable GM10115 human-hamster hybrid clones secrete factors that can perpetuate the instability phenotype over time. These clones do not have functional gap junctions, but do secrete significant amounts of Interleukin 8 (IL-8) into the culture medium. We then determined whether IL-8 could initiate and or perpetuate genomic instability over time in parental GM10115 cells. Contrary to our hypothesis, IL-8 could induce DNA damage, but was not responsible for the unstable phenotype. Instead it appears that IL-8 secretion provides a pro-survival function in cells that are chromosomally unstable and generally fail to thrive.
Laiakis
Evagelia C
EC
Radiation Oncology Research Laboratory, 655 West Baltimore Street, Bressler Research Building, Room 7-002, University of Maryland School of Medicine, Baltimore, MD 21201, United States. elaiakis@gmail.com
Baulch
Janet E
JE
Morgan
William F
WF
D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D002470:Cell Survival; D006224:Cricetinae; D004249:DNA Damage; D017629:Gap Junctions; D042822:Genomic Instability / Q000528:radiation effects*; D006801:Humans; D006822:Hybrid Cells; D016209:Interleukin-8 / Q000502:physiology*; D011839:Radiation, Ionizing; D017382:Reactive Oxygen Species / Q000032:analysis; D015398:Signal Transduction / Q000528:radiation effects
D016209:Interleukin-8; D017382:Reactive Oxygen Species