Expression of the translation initiation factors eIF-4E and eIF-2* is frequently increased in neoplastic cells of Hodgkin lymphoma.
The rate of protein synthesis is regulated in part by 2 key translation initiation factors, eIF-4E and eIF-2*. The expression and activity of both factors are increased transiently when normal resting cells are stimulated to proliferate, but they are constitutively elevated in oncogene-transformed cultured cells. Overexpression of either initiation factor induces a tumorigenic phenotype in rodent cells. We have shown earlier that expression of both eIF-4E and eIF-2* is increased in non-Hodgkin lymphomas (non-HLs). In this study, we performed an immunohistochemical survey of these translation initiation factors in neoplastic cells of HL. We also used Western blot to addressed the possibility that eIF-4E increases expression of NFkappaB. Our results indicate that both eIF-4E and eIF-2* are strongly expressed in neoplastic cells of HL in most cases examined as compared with weak or undetectable expression in most surrounding lymphocytes. An increase in eIF-4E expression may lead to constitutively high expression of NFkappaB, a transcription factor implicated in resistance to apoptosis and induction of cytokine gene expression in various cells, including neoplastic cells of HL.
Rosenwald
Igor B
IB
Department of Pathology, New England Medical Center, Boston, MA 02111, USA. irozenvald@tufts-nemc.org
Koifman
Larisa
L
Savas
Lou
L
Chen
Jane-Jane
JJ
Woda
Bruce A
BA
Kadin
Marshall E
ME
D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D041681:NIH 3T3 Cells
D014408:Biomarkers, Tumor; D015852:Eukaryotic Initiation Factor-2; D039561:Eukaryotic Initiation Factor-4E; D016328:NF-kappa B