Inner membrane lipids of Escherichia coli form domains.
In prokaryotic cells, the hypothesis of the existence of lipid domains was considered. In order to test this hypothesis and study the organization of lipids in the inner membrane of Escherichia coli, we elaborated Langmuir films mimicking the inner leaflet of this membrane by considering lipids extracted from the inner membrane of E coli by Folch protocol. Lipid monolayers were elaborated by using these extracts (Langmuir technique); the organization of the resulting films was studied at the air-water interface by Brewster angle microscopy and after transfer onto muscovite by atomic force microscopy. The existence of domains was demonstrated for different interfacial pressures of biological interest, and their stability was studied.
Zerrouk
Zakia
Z
Polymères, Biopolymères, Membranes, UMR 6522 CNRS, Université de Rouen, UFR des Sciences, 76821 Mont Saint Aignan Cedex, France.
Alexandre
Stéphane
S
Lafontaine
Céline
C
Norris
Vic
V
Valleton
Jean-Marc
JM
D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D008853:Microscopy / Q000379:methods; D018625:Microscopy, Atomic Force
D008563:Membrane Lipids