Epithelial cell rests of Malassez and OX6-immunopositive cells in the periodontal ligament of rat molars: a light and transmission electron microscope study.
It has been shown that human and cat epithelial cell rests of Malassez (ERM) consist of heterogeneous cell populations. Immunohistochemical and immunoelectron microscopic analyses have verified the presence of neuroendocrine and Merkel-like cells in both of these epithelia. During experimental orthodontic tooth movement, immunocompetent cells have also been found in the vicinity of ERM in rat periodontal ligament (PDL), but have not been characterized in normal rat PDL. The aim of this study was to investigate the presence and distribution of MHC class II antigen presenting cells by using OX6 antibody in ERM of rat molars by light and transmission electron microscopy. Immunohistochemical and immunoelectron microscopic observations of rat maxillary molars confirmed the presence of OX6-positive cells in contact with ERM. Some immunopositive cytoplasmic processes containing vesicles interdigitated with cells of the Malassez epithelial clusters. Based on these findings it can be concluded that immunocompetent cells are localized close to Malassez epithelial clusters in normal rat PDL. Furthermore, the ultrastructural evidences indicate a possible interaction between the epithelial and immunocompent cells and suggest morphological and functional properties for ERM.
Tadokoro
Osamu
O
Department of Oral Anatomy 1, Matsumoto Dental University School of Dentistry, Nagano, Japan. osamut@po.mdu.ac.jp
Vandevska-Randunovic
Vaska
V
Radunovic
Vaska
V
Inoue
Katsuhiro
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000648:ultrastructure; D002450:Cell Communication; D022162:Cytoplasmic Vesicles / Q000276:immunology / Q000648:ultrastructure; D004847:Epithelial Cells / Q000276:immunology / Q000648:ultrastructure; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D008963:Molar / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D010513:Periodontal Ligament / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; C116337:OX-6 monoclonal antibody