In vitro analysis of peroxisomal protein import.
This unit describes a quantitative in vitro assay for peroxisomal protein import that accurately reconstitutes established properties of the pathway. The cell-free system is ELISA based and employs semi-permeabilized human cells with a biotinylated peroxisomal targeting signal-containing substrate.
Terlecky
Stanley R
SR
Wayne State University School of Medicine, Detroit, Michigan, USA.
D000818:Animals; D001710:Biotin / Q000378:metabolism; D002463:Cell Membrane Permeability; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism* / Q000502:physiology; D051381:Rats
D001710:Biotin; D008156:Luciferases