title: The 20-minute rapid MART-1 immunostain for malignant melanoma frozen sections.
abstract: BACKGROUND
  Immunohistochemical staining has been used to help detect malignant melanoma on Mohs surgery frozen sections. Previous investigators have developed protocols for reliable MART-1 immunostaining of frozen sections, but these protocols are time-consuming.
  
  
  OBJECTIVE
  The objective was to report a rapid 20-minute MART-1 immunostaining protocol for frozen sections.
  
  
  METHODS
  The protocol was utilized on 30 melanomas treated with Mohs micrographic surgery.
  
  
  RESULTS
  The stain clearly highlighted normal background melanocytes, as well as melanocytic hyperplasia and malignant melanoma.
  
  
  CONCLUSIONS
  The 20-minute protocol provides a rapid and reliable method for immunostaining of malignant melanoma. The availability of more rapid immunostaining methods improves efficiency of the Mohs laboratory and significantly reduces patient and physician waiting time. The authors have indicated no significant interest with commercial supporters.
authors.affiliation: DermSurgery Associates, Houston, Texas 77030, USA. akimyai@yahoo.com
authors.forename: Arash
authors.initials: A
authors.lastname: Kimyai-Asadi
authors.forename: Gabriel B
authors.initials: GB
authors.lastname: Ayala
authors.forename: Leonard H
authors.initials: LH
authors.lastname: Goldberg
authors.forename: Justin
authors.initials: J
authors.lastname: Vujevich
authors.forename: Ming H
authors.initials: MH
authors.lastname: Jih
mesh_terms: D000951:Antigens, Neoplasm; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D058965:MART-1 Antigen; D008545:Melanoma / Q000473:pathology* / Q000601:surgery; D015580:Mohs Surgery; D009363:Neoplasm Proteins; D015203:Reproducibility of Results; D012878:Skin Neoplasms / Q000473:pathology* / Q000601:surgery; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D000951:Antigens, Neoplasm; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins