Haplotype analysis of ABCA3: association with respiratory distress in very premature infants.
BACKGROUND
Adenosine triphosphate (ATP)-binding cassette transporter A3 (ABCA3) gene mutations cause fatal respiratory failure in term infants, but common ABCA3 polymorphisms have remained uncharacterized at the population level.


OBJECTIVE
To define a subset of tagging single-nucleotide polymorphisms (tSNPs) which capture most of the variation within the ABCA3 gene, and to assess ABCA3 as a novel candidate gene for susceptibility to respiratory distress syndrome (RDS) in preterm infants.


METHODS
Based on an initial screen, nine tSNPs were selected. These 9 tSNPs and a length variation, representing > 90% of haplotypic variation of the gene, and 5 nonsynonymous coding SNPs were genotyped in 267 preterm infants. SNP rs13332514 was genotyped in an additional 48 infants.


RESULTS
The fourth common haplotype was overrepresented in very premature infants with RDS, being accounted for by SNP rs13332514 (F353F), with an increased minor allele frequency in RDS. Furthermore, rs13332514 associated significantly with chronic lung disease defined as a requirement for supplemental O2 at 28 postnatal days in very premature infants.


CONCLUSIONS
The results are suggestive of an association of a synonymous SNP in the ABCA3 gene with a prolonged course of respiratory distress syndrome in very premature infants and serve as a reference for further population-based studies of ABCA3.
Karjalainen
Minna K
MK
Department of Pediatrics and Biocenter Oulu, University of Oulu, Finland.
Haataja
Ritva
R
Hallman
Mikko
M
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D001997:Bronchopulmonary Dysplasia / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D012127:Respiratory Distress Syndrome, Newborn / Q000097:blood / Q000235:genetics*; D012189:Retrospective Studies
C441524:ABCA3 protein, human; D018528:ATP-Binding Cassette Transporters