title: Microsatellite-based phylogeny of Indian domestic goats.
abstract: BACKGROUND
  The domestic goat is one of the important livestock species of India. In the present study we assess genetic diversity of Indian goats using 17 microsatellite markers. Breeds were sampled from their natural habitat, covering different agroclimatic zones.
  
  
  RESULTS
  The mean number of alleles per locus (NA) ranged from 8.1 in Barbari to 9.7 in Jakhrana goats. The mean expected heterozygosity (He) ranged from 0.739 in Barbari to 0.783 in Jakhrana goats. Deviations from Hardy-Weinberg Equilibrium (HWE) were statistically significant (P < 0.05) for 5 loci breed combinations. The DA measure of genetic distance between pairs of breeds indicated that the lowest distance was between Marwari and Sirohi (0.135). The highest distance was between Pashmina and Black Bengal. An analysis of molecular variance indicated that 6.59% of variance exists among the Indian goat breeds. Both a phylogenetic tree and Principal Component Analysis showed the distribution of breeds in two major clusters with respect to their geographic distribution.
  
  
  CONCLUSIONS
  Our study concludes that Indian goat populations can be classified into distinct genetic groups or breeds based on the microsatellites as well as mtDNA information.
authors.affiliation: Centre for cellular and Molecular biology, Uppal Road, Hyderabad-500007, India. rout_ctc@hotmail.com
authors.forename: Pramod K
authors.initials: PK
authors.lastname: Rout
authors.forename: Manjunath B
authors.initials: MB
authors.lastname: Joshi
authors.forename: Ajoy
authors.initials: A
authors.lastname: Mandal
authors.forename: D
authors.initials: D
authors.lastname: Laloe
authors.forename: Lalji
authors.initials: L
authors.lastname: Singh
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Thangaraj
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000829:Animals, Domestic / Q000145:classification* / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D006041:Goats / Q000145:classification* / Q000235:genetics*; D007194:India; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D025341:Principal Component Analysis; D013045:Species Specificity
chemical_list: D004272:DNA, Mitochondrial