Analysis of the cell cycle using Xenopus egg extracts.
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in the extract fuse to assemble nuclei, making the extract suitable for studies of DNA replication and nuclear transport.
Kornbluth
Sally
S
Duke University Medical Center, Durham, North Carolina, USA.
Yang
Jing
J
Powers
Maureen
M
D021581:Active Transport, Cell Nucleus / Q000235:genetics; D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D002457:Cell Extracts / Q000138:chemical synthesis*; D002458:Cell Fractionation / Q000379:methods*; D004261:DNA Replication / Q000235:genetics; D005260:Female; D007399:Interphase / Q000187:drug effects / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D022161:Transport Vesicles / Q000502:physiology / Q000648:ultrastructure; D014982:Xenopus laevis / Q000502:physiology*
D002457:Cell Extracts