[Effects of a novel gene NOR(1) on the protein expression of HepG2 cell line].
OBJECTIVE
To investigate the mechanism of NOR(1) gene, a novel gene associated with liver cancer.


METHODS
NOR(1) was introduced into HepG2 cells by liposome transfection. After staining and image analysis, 6 differential expression spots which were up-regulated in NOR(1) transfected cells were isolated and identified by two-dimensional polyacrylamide gel eletrophoresis (2D PAGE) and MALDI-TOF.


RESULTS
The proteins which included zinc finger protein, tumor necrosis factor receptor, and protein tyrosine phosphatase receptor were involved in gene transcription and signal transduction associated with cancer.


CONCLUSIONS
NOR(1) gene may have some effects on liver cancer by up-regulating the expression of these proteins.
Nie
Xin-min
XM
Clinical Laboratory, Central South University, Changsha 410013, China. niexinmin7440@hotmail.com
Zhou
Jian-da
JD
Huang
Zhu-ying
ZY
Gui
Rong
R
Li
Deng-qing
DQ
Tang
Hua
H
D015152:Blotting, Northern; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D026901:Membrane Transport Proteins; C479458:OSCP1 protein, human; D018124:Receptors, Tumor Necrosis Factor