title: The histone deacetylase inhibitor butyrate inhibits melanoma cell invasion of Matrigel.
abstract: BACKGROUND
  Histone deacetylase (HDAC) inhibitors have anticancer effects. Their effects on expression of cell adhesion molecules might be related to their effects on tumor cell invasion.
  
  
  METHODS
  Murine B16-BL6 cells were treated with the HDAC inhibitors, butyrate or trichostatin A. Melanoma cell invasion of the artificial basement membrane, Matrigel, was examined by Transwell chamber assay.
  
  
  RESULTS
  Butyrate as well as trichostatin A inhibited the cell growth mainly by arresting the cell cycle. The cell invasion of Matrigel was inhibited by butyrate and trichostatin A. The butyrate treatment increased the cell-cell aggregation, although neither E-cadherin nor N-cadherin mRNA were up-regulated. Both mRNA expression and protein levels of the immunoglobulin superfamily cell adhesion molecules, Mel-CAM and L1-CAM, were increased in the butyrate-treated cells.
  
  
  CONCLUSIONS
  The HDAC inhibitor butyrate blocked the B16-BL6 melanoma cell invasion of Matrigel, although it increased the expression of Mel-CAM and L1-CAM which are important to the metastatic potential.
authors.affiliation: Molecular Biology Laboratory, Akita Prefectural University, Akita 010-0195, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kuwajima
authors.forename: Jun
authors.initials: J
authors.lastname: Iwashita
authors.forename: Jun
authors.initials: J
authors.lastname: Murata
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Abe
mesh_terms: D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D003094:Collagen; D004338:Drug Combinations; D004791:Enzyme Inhibitors / Q000494:pharmacology; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids / Q000494:pharmacology; D007797:Laminin; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D051379:Mice; D009361:Neoplasm Invasiveness; D011509:Proteoglycans
chemical_list: D002087:Butyrates; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; C012589:trichostatin A; D003094:Collagen