Induction of COX-2 expression by acrolein in the rat model of hemorrhagic cystitis.
OBJECTIVE
Acrolein (ACR) is a urinary metabolite of cyclophosphamide (CPS) and ifosfamide (IFS), which has been demonstrated to be the causative agent of hemorrhagic cystitis (HC), induced by these compounds. In this study, we investigate the participation of cyclooxygenase-2 (COX-2) on ACR-induced HC.


METHODS
Male Wistar rats (150-200g; six rats per group) were treated with distilled water or intravesical ACR and analyzed by changes in bladder wet weight, macroscopic and microscopic parameters and COX-2 expression.


RESULTS
COX-2 immunohistochemical expression was significant 12h after ACR administration mainly in subepithelial cells. ACR injection also alters some macroscopic and microscopic parameters in bladder of rats analyzed by Gray's criteria.


CONCLUSIONS
COX-2 participates in the pathogenesis of ACR-induced HC first seen 12h after initial contact between ACR and urothelium.
Macedo
Francisco Yuri Bulcão
FY
Department of Physiology and Pharmacology, Federal University of Ceara, Rua Coronel Nunes de Melo, 1315, Rodolfo Teofilo, 60430-270 fortaleza, CE, Brazil. ribeiror@ufc.br
Baltazar
Fátima
F
Mourão
Lívia Cajaseiras
LC
Almeida
Paulo Roberto Carvalho
PR
Mota
José Mauricio S C
JM
Schmitt
Fernando C
FC
Ribeiro
Ronaldo A
RA
D000171:Acrolein / Q000378:metabolism / Q000633:toxicity*; D000283:Administration, Intravesical; D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D003556:Cystitis / Q000139:chemically induced* / Q000150:complications / Q000201:enzymology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D006470:Hemorrhage / Q000139:chemically induced* / Q000150:complications / Q000201:enzymology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
D000171:Acrolein; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat