Magnetic particle-based sandwich sensor with DNA-modified carbon nanotubes as recognition elements for detection of DNA hybridization.
In this contribution, we design a visual sensor for DNA hybridization with DNA probe-modified magnetic particles (MPs) and multiwalled carbon nanotubes (MWNTs) without involving a visual recognition element such as fluorescent/chemiluminescent reagents. It was found that DNA probe-modified MWNTs, which could be dispersed in aqueous medium and have strong light scattering signals under the excitation of a light beam in the UV-vis region, could connect with DNA probe-modified MPs together in the presence of perfectly complementary target DNA and form a sandwich structure. In a magnetic field, the formed MP-MWNT species can easily be removed from the solution, resulting in a decrease of light scattering signals. Thus, a magnetic particle-based sandwich sensor could be developed to detect DNA hybridization by measuring the light scattering signals with DNA-modified MWNTs as recognition elements. Experiments showed that the DNA-modified MPs sensor could be reused at least 17 times and was stable for more than 6 months.
Hu
Po
P
College of Chemistry and Chemical Engineering, CQKL-LRTA, College of Life Sciences, Southwest University, Chongqing 400715, People's Republic of China.
Huang
Cheng Zhi
CZ
Li
Yuan Fang
YF
Ling
Jian
J
Liu
Yu Ling
YL
Fei
Liang Run
LR
Xie
Jian Ping
JP
D015374:Biosensing Techniques / Q000295:instrumentation*; D015342:DNA Probes / Q000737:chemistry*; D008280:Magnetics; D037742:Nanotubes, Carbon / Q000737:chemistry*; D045162:Thiazolidinediones / Q000737:chemistry*
D015342:DNA Probes; D037742:Nanotubes, Carbon; D045162:Thiazolidinediones; C114240:DN 108