Antibacterial activity of pyrrolidine dithiocarbamate.
Pyrrolidine dithiocarbamate (PDTC), an antioxidant with a metal-chelating activity, has been used widely to inhibit the expression of inflammatory genes in vitro and in vivo. This study investigated whether PDTC has an antimicrobial activity against various bacteria. The antibacterial activity of PDTC and other compounds was evaluated in vitro by the broth microdilution method against Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Staphylococcus aureus, and Escherichia coli. Bacterial growth was inhibited by PDTC, where a wide range of sensitivity was demonstrated among the tested bacteria. The antibacterial activity of PDTC was reduced by the addition of copper chloride; in contrast, it was enhanced considerably by zinc chloride. Two different zinc chelators, Ca-saturated EDTA (Ca-EDTA) and N,N,N',N'-tetrakis (2-pyridylmethyl) ethylenediamine, blocked the antibacterial activity of PDTC, whereas Zn-EDTA failed to reduce the activity of PDTC. These results demonstrate for the first time that PDTC possesses an antibacterial activity, for which zinc is required, and suggest that PDTC, possessing a dual anti-inflammatory and antibacterial activity, may be considered for topical use for inflammatory diseases of bacterial origin.
Kang
Mi-Sun
MS
Brain Korea 21 Program, Chonnam National University, Kwangju, South Korea.
Choi
Eun-Kyoung
EK
Choi
Do-Hyun
DH
Ryu
Sun-Youl
SY
Lee
Hye-Hyang
HH
Kang
Ho-Cheol
HC
Koh
Jeong-Tae
JT
Kim
Ok-Su
OS
Hwang
Yun-Chan
YC
Yoon
Suk-Ja
SJ
Kim
Seon-Mi
SM
Yang
Kyu-Ho
KH
Kang
In-Chol
IC
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D002614:Chelating Agents / Q000493:pharmacokinetics*; D002712:Chlorides / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology*; D013859:Thiocarbamates / Q000494:pharmacology*; D017967:Zinc Compounds / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D002712:Chlorides; D011759:Pyrrolidines; D013859:Thiocarbamates; D017967:Zinc Compounds; C020972:pyrrolidine dithiocarbamic acid; D003300:Copper; C016837:zinc chloride