title: Cellular and molecular determinants targeting the Caenorhabditis elegans PHR protein RPM-1 to perisynaptic regions.
abstract: Caenorhabditis elegans RPM-1 is a member of a conserved protein family, the PHR proteins, that includes human Pam, mouse Phr1, zebrafish Esrom, and Drosophila Highwire. PHR proteins play important roles in the development of the nervous system. In particular, mutations in rpm-1 cause a disruption of synaptic architecture, affecting the distribution of synaptic vesicles and the number of presynaptic densities. Using antibodies against RPM-1, we determined the localization of the endogenous RPM-1 protein in wild-type and in several mutants that affect synaptic development. Our analyses show that, in mature neurons, RPM-1 resides in a distinct region that is close to, but does not overlap with, the synaptic exo- and endocytosis domains. The localization of RPM-1 occurs independently of several proteins that function in the transport or assembly of synapse components, and its abundance is partially dependent on its binding partner the F-box protein FSN-1. RPM-1 has been shown to target the MAPKKK DLK-1 for degradation. We show that activated DLK-1 may be preferentially targeted for degradation. Furthermore, using transgene analysis, we identified a critical role of the conserved PHR domain of RPM-1 in its subcellular localization.
authors.affiliation: Department of Molecular, Cell and Developmental Biology, Sinsheimer Laboratories, University of California Santa Cruz, Santa Cruz, California, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Abrams
authors.forename: Brock
authors.initials: B
authors.lastname: Grill
authors.forename: Xun
authors.initials: X
authors.lastname: Huang
authors.forename: Yishi
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D034284:Dynamin I / Q000378:metabolism*; D044783:F-Box Proteins / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D020930:MAP Kinase Kinase Kinases / Q000378:metabolism*; D009154:Mutation; D009474:Neurons / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D013569:Synapses / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D044783:F-Box Proteins; D020662:Guanine Nucleotide Exchange Factors; C409634:RPM-1 protein, C elegans; C498050:DLK-1 protein, C elegans; D020930:MAP Kinase Kinase Kinases; D034284:Dynamin I