Alkaline phosphatase, glutathione-S-transferase-P, and cofilin-1 distinguish multipotent mesenchymal stromal cell lines derived from the bone marrow versus peripheral blood.
Multipotent mesenchymal stromal cells (MSCs) can be isolated from bone marrow or peripheral blood. To identify phenotypical and functional differences between MSCs derived from these sources, the human bone marrow-derived, fibroblast-like cell line L87/4 was compared with the peripheral blood-derived, fibroblast-like cell line V54/2. Both cell lines expressed similar levels of SH3+, CD45(-), CD68(-), CD133(-), and HLA-DR(-). The bone marrow-derived cells expressed higher surface levels of CD105, CD10, and CD117 and preferentially expressed alkaline phosphatase, glutathione S-transferase P, and cofilin-1. The peripheral blood-derived line showed a higher number of CD34+/CD105+ double-positive and side population (SP) cells. The results demonstrate the more multipotent, yet quiescent, stromal phenotype of bone marrow MSCs, whereas MSCs isolated from the circulation display more hematopoietic-lineage characteristics. Importantly, potential marker genes that distinguish the two stages of MSCs are defined.
Conrad
Claudius
C
Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA. cconrad1@partners.org
Zeindl-Eberhart
Evelyn
E
Moosmann
Sabine
S
Nelson
Peter J
PJ
Bruns
Christiane J
CJ
Huss
Ralf
R
D000469:Alkaline Phosphatase / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells; D001854:Bone Marrow Cells; D002460:Cell Line; D051338:Cofilin 1 / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D059630:Mesenchymal Stem Cells; D039902:Multipotent Stem Cells; D017154:Stromal Cells / Q000166:cytology*
D015415:Biomarkers; D051338:Cofilin 1; D005982:Glutathione Transferase; D000469:Alkaline Phosphatase