Development of recombinant canine adenovirus type-2 expressing the Gn glycoprotein of Seoul virus.
Seoul virus glycoprotein Gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. In this study, a replication-competent recombinant canine adenovirus type-2 expressing Gn was constructed by the in vitro ligation method. The Gn expression cassette, including the human cytomegalovirus (hCMV) promoter/enhancer and the SV40 early mRNA polyadenylation signal, was cloned into the SspI site of the E3 region which is not essential for proliferation of CAV-2. Expression of Gn was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.
Yuan
Ziguo
Z
Laboratory of Epidemiology, Veterinary Institute, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun 130062, PR China.
Zhang
Xiuxiang
X
Zhang
Shoufeng
S
Liu
Ye
Y
Gao
Shengyan
S
Zhang
Fei
F
Xu
Huijuan
H
Wang
Xiaohu
X
Hu
Rongliang
R
D006516:Adenoviruses, Canine / Q000378:metabolism*; D000818:Animals; D001671:Biochemistry / Q000379:methods; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics; D004285:Dogs; D005821:Genetic Techniques; D005822:Genetic Vectors; D006023:Glycoproteins / Q000378:metabolism*; D006476:Orthohantavirus / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029263:Seoul virus / Q000378:metabolism*
D006023:Glycoproteins