The pap1(+) gene of fission yeast is transcriptionally regulated by nitrosative and nutritional stress.
In the current work, regulation of the pap1(+) gene was investigated by the use of the pap1(+)-lacZ fusion gene and semi-quantitative reverse transcriptase-PCR. The synthesis of beta-galactosidase from the pap1(+)-lacZ fusion gene was significantly enhanced by nitric oxide (NO)-generating sodium nitroprusside (SNP) and nitrogen starvation. However, the induction by SNP and nitrogen starvation was observed to be much less in the Pap1p-negative cells harboring the fusion gene. Exogenous NO was more effectively scavenged in the Pap1p-positive cells than in the Pap1p-negative cells. Oxidative stress such as superoxide anion, hydrogen peroxide and cadmium could not give rise to an effect on the synthesis of beta-galactosidase from the fusion gene. The pap1(+) mRNA level was elevated in the wild-type cells by SNP and nitrogen starvation. Catalase activity, a major enzyme positively regulated by Pap1p, was significantly increased only in the Pap1p-positive cells by SNP. In brief, it is demonstrated that transcription of the Schizosaccharomyces pombe pap1(+) gene is positively regulated by nitrosative and nutritional stress in a Pap1p-dependent manner.
Kim
Hyeon-Jung
HJ
Division of Life Sciences and Research Institute of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon, Korea.
Jung
Ha-Yun
HY
Lim
Chang-Jin
CJ
D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D009566:Nitrates / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D015538:Nitrosation; D015596:Nutrition Assessment; D000073718:Pancreatitis-Associated Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
D050976:Basic-Leucine Zipper Transcription Factors; D009566:Nitrates; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D029702:Schizosaccharomyces pombe Proteins; D009584:Nitrogen