Preparation of cytogenetic specimens from tissue samples.
Cytogenetic specimens are prepared from short-term primary cultures established from tissue samples or from established cell lines. Cultures that are (80% confluent are treated with Colcemid to enrich for cells in mitosis. Cells are then swollen hypotonically and fixed before slides are prepared. Preparing good metaphase spreads is technically challenging, This unit provides a good discussion of the factors that go into making a good preparation.
Bayani
Jane
J
University of Toronto, Ontario, Canada.
Squire
Jeremy A
JA
D001773:Blood Cells / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002877:Chromosomes, Human / Q000737:chemistry; D020732:Cytogenetic Analysis / Q000379:methods*; D006801:Humans; D008677:Metaphase / Q000502:physiology; D008954:Models, Biological; D013048:Specimen Handling / Q000379:methods*