title: Molecular diversity of bacterial communities from subseafloor rock samples in a deep-water production basin in Brazil.
abstract: The deep subseafloor rock in oil reservoirs represents a unique environment in which a high oilcontamination and very low biomass can be observed. Sampling this environment has been a challenge owing to the techniques used for drilling and coring. In this study, the facilities developed by the Brazilian oil company PETROBRAS for accessing deep subsurface oil reservoirs were used to obtain rock samples at 2,822-2,828 m below the ocean floor surface from a virgin field located in the Atlantic Ocean, Rio de Janeiro. To address the bacterial diversity of these rock samples, PCR amplicons were obtained using the DNA from four core sections and universal primers for 16S rRNA and for APS reductase (aps) genes. Clone libraries were generated from these PCR fragments and 87 clones were sequenced. The phylogenetic analyses of the 16S rDNA clone libraries showed a wide distribution of types in the domain bacteria in the four core samples, and the majority of the clones were identified as belonging to Betaproteobacteria. The sulfate-reducing bacteria community could only be amplified by PCR in one sample, and all clones were identified as belonging to Gammaproteobacteria. For the first time, the bacterial community was assessed in such deep subsurface environment.
authors.affiliation: Instituto de Microbiologia Prof. Paulo de Goes, UFRJ, CCS, Bloco I, Ilha do Fundao, Rio de Janeiro - RJ, Brazil.
authors.forename: Irene
authors.initials: I
authors.lastname: von der Weid
authors.forename: Elisa
authors.initials: E
authors.lastname: Korenblum
authors.forename: Diogo
authors.initials: D
authors.lastname: Jurelevicius
authors.forename: Alexandre Soares
authors.initials: AS
authors.lastname: Rosado
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Dino
authors.forename: Gina Vasquez
authors.initials: GV
authors.lastname: Sebastian
authors.forename: Lucy
authors.initials: L
authors.lastname: Seldin
mesh_terms: D001267:Atlantic Ocean; D001938:Brazil; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D017753:Ecosystem; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics; D010578:Petroleum; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000378:metabolism; D016999:Sulfur-Reducing Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase