title: Centromere anatomy in the multidrug-resistant pathogen Enterococcus faecium.
abstract: Multidrug-resistant variants of the opportunistic human pathogen Enterococcus have recently emerged as leading agents of nosocomial infection. The acquisition of plasmid-borne resistance genes is a driving force in antibiotic-resistance evolution in enterococci. The segregation locus of a high-level gentamicin-resistance plasmid, pGENT, in Enterococcus faecium was identified and dissected. This locus includes overlapping genes encoding PrgP, a member of the ParA superfamily of segregation proteins, and PrgO, a site-specific DNA binding homodimer that recognizes the cenE centromere upstream of prgPO. The centromere has a distinctive organization comprising three subsites, CESII separates CESI and CESIII, each of which harbors seven TATA boxes spaced by half-helical turns. PrgO independently binds both CESI and CESIII, but with different affinities. The topography of the complex was probed by atomic force microscopy, revealing discrete PrgO foci positioned asymmetrically at the CESI and CESIII subsites. Bending analysis demonstrated that cenE is intrinsically curved. The organization of the cenE site and of certain other plasmid centromeres mirrors that of yeast centromeres, which may reflect a common architectural requirement during assembly of the mitotic apparatus in yeast and bacteria. Moreover, segregation modules homologous to that of pGENT are widely disseminated on vancomycin and other resistance plasmids in enterococci. An improved understanding of segrosome assembly may highlight new interventions geared toward combating antibiotic resistance in these insidious pathogens.
authors.affiliation: Faculty of Life Sciences and Manchester Interdisciplinary Biocentre, University of Manchester, Manchester M1 7DN, United Kingdom.
authors.forename: Andrew
authors.initials: A
authors.lastname: Derome
authors.forename: Christian
authors.initials: C
authors.lastname: Hoischen
authors.forename: Malte
authors.initials: M
authors.lastname: Bussiek
authors.forename: Ruth
authors.initials: R
authors.lastname: Grady
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Adamczyk
authors.forename: Barbara
authors.initials: B
authors.lastname: Kedzierska
authors.forename: Stephan
authors.initials: S
authors.lastname: Diekmann
authors.forename: Daniela
authors.initials: D
authors.lastname: Barillà
authors.forename: Finbarr
authors.initials: F
authors.lastname: Hayes
mesh_terms: D002503:Centromere; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D016984:Enterococcus faecium / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data