title: Scanning electron microscopy of cell surface morphology.
abstract: The surface of metazoan cells is a landscape not clearly visualized by light microscopy. Many cells elaborate protrusive structures such as microvilli, filopodia, lamellipodia, and surface ruffles that play important roles in the interaction between the cell and its environment. The high resolution of scanning electron microscopy makes it an ideal technique for studies of the cell surface; however, preservation of fine surface structure can be problematic. Here we highlight the critical factors in sample preparation to ensure optimal high-resolution imaging of the surface of mammalian cells and tissues.
authors.affiliation: Mammalian Cell Biology Laboratory, Department of Biochemistry, School of Medical Sciences, University of Bristol, United Kingdom.
authors.forename: Samantha
authors.initials: S
authors.lastname: Passey
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Pellegrin
authors.forename: Harry
authors.initials: H
authors.lastname: Mellor
mesh_terms: D000818:Animals; D002450:Cell Communication; D022081:Cell Surface Extensions / Q000648:ultrastructure*; D004847:Epithelial Cells / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods* / Q000639:trends; D008871:Microvilli / Q000648:ultrastructure; D011554:Pseudopodia; D014021:Tissue Preservation / Q000295:instrumentation / Q000379:methods* / Q000639:trends