Immunoprecipitation.
Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody.
Bonifacino
J S
JS
National Institute of Child Health and Human Development, Bethesda, Maryland, USA.
Dell'Angelica
E C
EC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D002478:Cells, Cultured / Q000737:chemistry; D003902:Detergents; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D047468:Immunoprecipitation / Q000295:instrumentation / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*; D015531:Radioimmunoprecipitation Assay / Q000379:methods; D011868:Radioisotopes / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D013048:Specimen Handling
D000911:Antibodies, Monoclonal; D003902:Detergents; D005656:Fungal Proteins; D011506:Proteins; D011868:Radioisotopes