title: Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria.
abstract: We describe the development of a miniaturised microarray for the detection of antimicrobial resistance genes in Gram-negative bacteria. Included on the array are genes encoding resistance to aminoglycosides, trimethoprim, sulphonamides, tetracyclines and beta-lactams, including extended-spectrum beta-lactamases. Validation of the array with control strains demonstrated a 99% correlation between polymerase chain reaction and array results. There was also good correlation between phenotypic and genotypic results for a large panel of Escherichia coli and Salmonella isolates. Some differences were also seen in the number and type of resistance genes harboured by E. coli and Salmonella strains. The array provides an effective, fast and simple method for detection of resistance genes in clinical isolates suitable for use in diagnostic laboratories, which in future will help to understand the epidemiology of isolates and to detect gene linkage in bacterial populations.
authors.affiliation: Department of Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey, UK.
authors.forename: Miranda
authors.initials: M
authors.lastname: Batchelor
authors.forename: Katie L
authors.initials: KL
authors.lastname: Hopkins
authors.forename: Ernesto
authors.initials: E
authors.lastname: Liebana
authors.forename: Peter
authors.initials: P
authors.lastname: Slickers
authors.forename: Ralf
authors.initials: R
authors.lastname: Ehricht
authors.forename: Muriel
authors.initials: M
authors.lastname: Mafura
authors.forename: Frank
authors.initials: F
authors.lastname: Aarestrup
authors.forename: Dik
authors.initials: D
authors.lastname: Mevius
authors.forename: Felicity A
authors.initials: FA
authors.lastname: Clifton-Hadley
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: Rob H
authors.initials: RH
authors.lastname: Davies
authors.forename: E John
authors.initials: EJ
authors.lastname: Threlfall
authors.forename: Muna F
authors.initials: MF
authors.lastname: Anjum
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013223:Statistics as Topic
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial