Src-Tyrosine kinases are major agents in mitochondrial tyrosine phosphorylation.
Mitochondrial tyrosine phosphorylation is emerging as an important mechanism in regulating mitochondrial function. This article, aimed at identifying which kinases are the major agents in mitochondrial tyrosine phosphorylation, shows that this role should be attributed to Src family members. Indeed, various members of this family, for example, Fgr, Fyn, Lyn, c-Src, are constitutively present in the internal structure of mitochondria as well as Csk, a key enzyme in the regulation of the activity of this family. By means of different approaches, biochemical fractioning, Western blotting and immunogold analysis "in situ" of phosphotyrosine signaling, evidence is reported on the existence of a signal transduction pathway from plasma membrane to mitochondria, resulting in increasing Src-dependent mitochondrial tyrosine phosphorylation. The activation of Src kinases at mitochondrial level is associated with the proliferative status where several mitochondrial proteins are specifically tyrosine-phosphorylated.
Tibaldi
Elena
E
Dipartimento di Chimica Biologica, Universita' di Padova, Istituto di Neuroscienze del C.N.R., Via G. Colombo 3, 35121 Padova, Italy.
Brunati
Anna Maria
AM
Massimino
Maria Lina
ML
Stringaro
Annarita
A
Colone
Marisa
M
Agostinelli
Enzo
E
Arancia
Giuseppe
G
Toninello
Antonio
A
D000818:Animals; D001921:Brain / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D015398:Signal Transduction; D014443:Tyrosine / Q000737:chemistry*; D019061:src-Family Kinases / Q000378:metabolism*
D014443:Tyrosine; D019061:src-Family Kinases