title: Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model.
abstract: Measles virus (MeV) vaccine strain, AIK-C, is temperature sensitive (ts), which is thought to be associated with attenuation of virus pathogenicity. In this study, replication and antibody response were examined in cotton rats using viruses carrying different forms of the P gene, which is responsible for the ts phenotype of strain AIK-C and its parental Edmonston strain. When cotton rats were inoculated intranasally, ts viruses neither replicated in lungs, nor reproducibly generated an antibody response. When inoculated intramusculary (i.m.), however, ts strains raised an antibody titer in all animals. This response was not observed when ultraviolet-inactivated virus was used. ts virus, inoculated i.m., was recovered from cotton rat drainage lymph nodes. These results suggest that ts virus, inoculated i.m., could replicate in the cotton rat, presumably at the superficial lymph node, and induce an antibody response. Therefore, cotton rats can serve as a small-animal model for investigating immune responses to safer ts vaccine, as well as recombinant vaccine using AIK-C as a vector for protection against other infectious agents.
authors.affiliation: Department of Veterinary Microbiology, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. a0d518u@cc.miyazaki-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haga
authors.forename: Niho
authors.initials: N
authors.lastname: Murayama
authors.forename: Yuya
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Akatsuki
authors.initials: A
authors.lastname: Saito
authors.forename: Takumi
authors.initials: T
authors.lastname: Sakamoto
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Morita
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Komase
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakayama
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Uchida
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Katayama
authors.forename: Akio
authors.initials: A
authors.lastname: Shinohara
authors.forename: Chihiro
authors.initials: C
authors.lastname: Koshimoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sato
authors.forename: Hironori
authors.initials: H
authors.lastname: Miyata
authors.forename: Kiyoaki
authors.initials: K
authors.lastname: Katahira
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Goto
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000276:immunology / Q000821:virology; D008198:Lymph Nodes / Q000276:immunology* / Q000821:virology; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D016205:Sigmodontinae / Q000276:immunology* / Q000821:virology; D013696:Temperature; D014779:Virus Replication / Q000276:immunology
chemical_list: D008458:Measles Vaccine