Cytogenetic sensitivity of G0 lymphocytes of Fanconi anemia patients and obligate carriers to mitomycin C and ionizing radiation.
It is well known that Fanconi anemia (FA) patients show a hypersensitivity to the effect of cross-linking agents such as mitomycin C (MMC) and diepoxybutane (DEB), while the sensitivity of these patients to ionizing radiation is still controversial. Fanconi anemia heterozygotes do not show a hypersensitivity to the above mentioned agents compared to normal individuals. To examine the radio-sensitivity of Fanconi anemia patients and heterozygotes, ten patients and 13 heterozygotes were enrolled in this study. Standard metaphase analysis for detection and verification of radio-sensitivity was used to establish the relationship between gamma-ray and chromosome breakages in these groups. Statistical analysis was used for the assessment of aberrations including chromatid and chromosome breaks and exchanges. Results of chromosome aberration yield that: (i) differentiation between obligate carriers and the control group after MMC treatment and gamma irradiation was not possible; (ii) homozygotes were clearly distinguishable from heterozygotes and controls after MMC treatment; (iii) FA patients don't show hypersensitivity to gamma irradiation compared to normal controls and heterozygous carriers.
Mohseni-Meybodi
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A
Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mozdarani
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H
Vosough
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P
D000328:Adult; D016022:Case-Control Studies; D002648:Child; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D003582:Cytogenetics; D005199:Fanconi Anemia / Q000235:genetics* / Q000473:pathology*; D005260:Female; D006579:Heterozygote; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D011839:Radiation, Ionizing
D016685:Mitomycin