Antiviral activity of the exopolysaccharide produced by Serratia sp. strain Gsm01 against Cucumber mosaic virus.
The potential of the exopolysaccharide (EPS) from a Serratia sp. strain Gsm01 as an antiviral agent against a yellow strain of Cucumber mosaic virus (CMV-Y) was evaluated in tobacco plants (Nicotiana tabacum cv. Xanthi-nc). The spray treatment of plants using an EPS preparation, 72 before CMV-Y inoculation, protected them against symptom appearance. Fifteen days after challenge inoculation with CMVY, 33.33% of plants showed mosaic symptoms in EPS-treated plants compared with 100% in the control plants. The EPS-treated plants, which showed mosaic symptoms, appeared three days later than the controls. The enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR) analyses of the leaves of the protected plants revealed that the EPS treatment affected virus accumulation in those plants. Analysis of phenylalanine ammonia lyase, peroxidase, and phenols in protected plants revealed enhanced accumulation of these substances. The pathogenesis-related (PR) genes expression represented by PR-1b was increased in EPS-treated plants. This is the first report of a systemic induction of protection triggered by EPS produced by Serratia sp. against CMV-Y.
Ipper
Nagesh S
NS
Laboratory of Bacterial Genetics and Biotechnology, Division of Bio-Resources Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Korea.
Cho
Saeyoull
S
Lee
Seon Hwa
SH
Cho
Jun Mo
JM
Hur
Jang Hyun
JH
Lim
Chun Keun
CK
D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology; D017799:Cucumovirus / Q000187:drug effects* / Q000472:pathogenicity; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000494:pharmacology*; D012705:Serratia / Q000378:metabolism*; D014026:Nicotiana / Q000821:virology*
D000998:Antiviral Agents; D011135:Polysaccharides, Bacterial