[Cloning and analysis of adenine nucleotide translocase gene in Helicoverpa armigera].
A cDNA clone encoding the ADP/ATP translocase in Helicoverpa armigera has been identified by RT-PCR, 5'and 3'RACE methods. Sequence analysis shows that it is 1,190 bp long and contains a single open reading frame (ORF, 133-1,033 bp) encoding a protein of 300 amino acids (GenBank submission number, AY253868). The protein has a 22 aa signal peptide on its N-terminal, which leads the protein locating onto the inner membrane of the mitochondria. It also has three conserved domains of the mitochondrial carrier protein forming a channel to exchange ATP and ADP energy molecule through the inner membrane of the mitochondria. It shows extensive similarities to the known ADP/ATP translocase poly-peptides. The ADP/ATP translocase similarity was up to 90% in the Lepidoptera.
Niu
Bao-Long
BL
Laboratory of Entomo-molecular Biology, Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. niubaolong@126.com
Weng
Hong-Biao
HB
He
Li-Hua
LH
Shen
Wei-Feng
WF
Qi
Xiao-Peng
XP
Liu
Yan
Y
Meng
Zhi-Qi
ZQ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000235:genetics; D007915:Lepidoptera / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D000226:Mitochondrial ADP, ATP Translocases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D000226:Mitochondrial ADP, ATP Translocases