Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay.
A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 microM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity.
Hwang
Yu-Chen
YC
Department of Environmental Sciences, University of California, Riverside, CA 92521, USA.
Chu
Justin Jang-Hann
JJ
Yang
Priscilla L
PL
Chen
Wilfred
W
Yates
Marylynn V
MV
D047151:Amaryllidaceae Alkaloids / Q000494:pharmacology; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity*; D002460:Cell Line; D002522:Chlorocebus aethiops; D031541:Fluorescence Resonance Energy Transfer; D019787:Linoleic Acid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008856:Microscopy, Fluorescence; D010447:Peptide Hydrolases / Q000378:metabolism; D010617:Phenanthridines / Q000494:pharmacology; D017955:Poliovirus / Q000187:drug effects*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D047151:Amaryllidaceae Alkaloids; D000998:Antiviral Agents; D010617:Phenanthridines; D014764:Viral Proteins; D000841:Anisomycin; D019787:Linoleic Acid; D010447:Peptide Hydrolases; C015330:lycorine