title: Overexpression of VvWRKY2 in tobacco enhances broad resistance to necrotrophic fungal pathogens.
abstract: WRKY genes encode proteins belonging to a large family of transcription factors that are involved in various developmental and physiological processes and in plant responses to pathogen infections. In the present work, a full-length cDNA from a Vitis vinifera L. cv. Cabernet Sauvignon grape berry library was isolated and characterized. The cDNA, designated VvWRKY2, encodes a polypeptide of 536 amino acids that shows the structural features of group I of WRKY protein family. VvWRKY2 is expressed in the different organs of healthy grapevine plants. In leaves, VvWRKY2 is induced by wounding and after infection with Plasmopara viticola. Constitutive expression of VvWRKY2 in tobacco reduced the susceptibility of transgenic tobacco to three types of fungal pathogens infecting different parts of the plant: Botrytis cinerea (leaves), Pythium spp. (roots) and Alternaria tenuis (seeds). The results indicate that VvWRKY2 may be involved in the resistance of grapevine against the pathogens.
authors.affiliation: Unité Mixte de Recherche 1287, Ecophysiologie et Génomique Fonctionnelle de la Vigne, Université Bordeaux 1, Institut des Sciences de la Vigne et du Vin, BP 81, 33883 Villenave d'Ornon, France.
authors.forename: Rim
authors.initials: R
authors.lastname: Mzid
authors.forename: Chloé
authors.initials: C
authors.lastname: Marchive
authors.forename: Dominique
authors.initials: D
authors.lastname: Blancard
authors.forename: Laurent
authors.initials: L
authors.lastname: Deluc
authors.forename: François
authors.initials: F
authors.lastname: Barrieu
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Corio-Costet
authors.forename: Nourredine
authors.initials: N
authors.lastname: Drira
authors.forename: Saïd
authors.initials: S
authors.lastname: Hamdi
authors.forename: Virginie
authors.initials: V
authors.lastname: Lauvergeat
mesh_terms: D000528:Alternaria / Q000254:growth & development; D000595:Amino Acid Sequence; D001483:Base Sequence; D020171:Botrytis / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000235:genetics; D003904:Mitosporic Fungi / Q000254:growth & development*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology; D027843:Vitis / Q000235:genetics*
chemical_list: D010940:Plant Proteins