Effects of letrozole and clomiphene citrate on the expression of HOXA10 and integrin alpha v beta 3 in uterine epithelium of rats.
OBJECTIVE
To compare the effects of letrozole and clomiphene citrate on the expression of HOXA10 and integrin alpha(v)beta(3) in the uterine epithelium in rats.


METHODS
Controlled prospective study.


METHODS
Teaching hospital and university research laboratory.


METHODS
Sixty sexually mature female Wistar-Albino rats that were 20 weeks of age.


METHODS
Letrozole, 5 mg/kg of body weight daily (20 rats); clomiphene citrate, 100 microg/kg daily (20 rats); and saline solution, 2 mL/day (20 rats). After 2 days, rats were killed and hysterectomized.


METHODS
Expression of integrin alpha(v)beta(3) and HOXA10.


RESULTS
The expression of integrin alpha(v)beta(3) in the clomiphene citrate group was statistically significantly lower than in the letrozole and saline solution groups. The expression of HOXA10 was statistically significantly higher in the saline solution group than in the letrozole group, and the letrozole group showed a statistically significantly higher expression of HOXA10 compared with the clomiphene citrate group.


CONCLUSIONS
In rats, letrozole affects the expression of HOXA10 in uterine epithelium but has no effect on the expression of integrin alpha(v)beta(3), which suggests that clomiphene suppresses endometrial receptivity more than letrozole. In the future, letrozole may be an appropriate drug for ovulation induction.
Bao
Shi Hua
SH
Department of Obstetrics and Gynecology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Sheng
Shi Le
SL
Peng
Yi Feng
YF
Lin
Qi De
QD
D000818:Animals; D002996:Clomiphene / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins / Q000187:drug effects / Q000235:genetics*; D007044:Hysterectomy; D039302:Integrin alphaVbeta3 / Q000187:drug effects / Q000235:genetics*; D000077289:Letrozole; D009570:Nitriles / Q000494:pharmacology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014230:Triazoles / Q000494:pharmacology*; D014599:Uterus / Q000187:drug effects / Q000502:physiology*
C534680:HOXA10 protein, rat; D000080905:Homeobox A10 Proteins; D018398:Homeodomain Proteins; D039302:Integrin alphaVbeta3; D009570:Nitriles; D014230:Triazoles; D002996:Clomiphene; D012313:RNA; D000077289:Letrozole