Radioiodination of cellular proteins.
Despite the advances of non-radioactive methods of protein labeling, radioiodination remains an important tool for studying proteins. This unit discusses several commonly used methods for radioiodination of proteins for various purposes. Included are several simple, reliable protocols for radioiodination of cell surface proteins on live cells, radioiodination of purified, solubilized membrane proteins, and radioiodination of purified soluble proteins.
Caplan
Steve
S
NICHD, National Institutes of Health, Bethesda, Maryland, USA.
Baniyash
Michal
M
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism*
D007457:Iodine Radioisotopes; D008565:Membrane Proteins