title: Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode.
abstract: Simultaneous determination of lactic acid, pyruvic acid, 3-hydroxybutyric acid and acetoacetic acid for clinical monitoring of lactic acidosis and ketone body formation in human plasma (20 microL) was performed by gas chromatography-mass spectrometry in selected ion monitoring (SIM) mode after generating methoxime/tert-butyldimethylsilyl derivatives. All of the targeted carboxylic acids were detected by characteristic fragment ions, which permitted sensitive and selective identification in the presence of co-extracted free fatty acids and other acidic metabolites at much higher levels. The method was linear (r>or=0.9991), reproducible (% relative standard deviation=1.2-5.8), and accurate (% relative error=-7.2-7.6), with detection limits of 0.05-1.7 ng/mL. This rapid, accurate and selective method using minimal plasma samples (20 microL) is useful in the clinical monitoring of lactic acidosis and ketone body formation in plasma.
authors.affiliation: Metabolomic Analysis Laboratory, Institute for Neuroregeneration and Stem Cell Research, Ajou University, Wonchon-dong, Yeongtong-gu, Suwon 443-721, South Korea.
authors.forename: Man-Jeong
authors.initials: MJ
authors.lastname: Paik
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Cho
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Kyoung-Rae
authors.initials: KR
authors.lastname: Kim
authors.forename: Sangdun
authors.initials: S
authors.lastname: Choi
authors.forename: Young-Hwan
authors.initials: YH
authors.lastname: Ahn
authors.forename: Gwang
authors.initials: G
authors.lastname: Lee
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007657:Ketone Bodies / Q000097:blood* / Q000737:chemistry; D019344:Lactic Acid / Q000097:blood* / Q000737:chemistry; D008297:Male; D017646:Organosilicon Compounds / Q000737:chemistry*; D010091:Oximes / Q000737:chemistry*; D019289:Pyruvic Acid / Q000097:blood* / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D007657:Ketone Bodies; D017646:Organosilicon Compounds; D010091:Oximes; C009660:t-butyldimethylsilyl compounds; D019344:Lactic Acid; C005514:N,N'-monomethylenebis(pyridiniumaldoxime); D019289:Pyruvic Acid