Expression of an activated form of integrin beta2 chain CD18 in cardiac surgical operations.
BACKGROUND
Myeloid cells are extensively activated in patients undergoing cardiosurgical operations. It is supposed that this activation is more profound in patients operated with cardiopulmonary bypass (CPB) ("on-pump") in comparison with patients operated without CPB ("off-pump").


OBJECTIVE
To evaluate changes in the expression of a novel activation marker expressed on myeloid cells recognized by MEM-148 antibody.


METHODS
The expression of MEM-148 positive myeloid cells was evaluated by flow cytometry in 40 patients who underwent coronary artery bypass surgery (CABG) randomly assigned to "on-pump" or "off-pump" technique.


RESULTS
The relative and absolute number of MEM-148 positive myeloid cells is significantly diminished during "on-pump" surgery. A significant increase in their number in postoperative period in both "on-pump" and "off-pump" patients was found. There were no significant differencies between "on-pump" and "off-pump" patients.


CONCLUSIONS
The very trauma of surgery seems to be more relevant in starting on activation of myeloid cells them CPB itself.
Kudlová
Manuela
M
Charles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Clinical Immunology and Allergology, Czech Republic.
Kolácková
Martina
M
Kunes
Pavel
P
Lonský
Vladimír
V
Mand'ák
Jirí
J
Ctirad
Andrýs
A
Krejsek
Jan
J
D000368:Aged; D018821:CD18 Antigens / Q000097:blood*; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D047549:Coronary Artery Bypass, Off-Pump; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008297:Male
D018821:CD18 Antigens