Detection of mitotic figures and components of the mitotic machinery.
Mitotic figures have provided important information on the mechanisms behind observed chromosomal aberrations. Variations in cell culturing and fixation methods enhance the visualization of mitotic figures. Furthermore, immunohistochemistry using a host of antibodies against protein structures involved in the cell cycle, as well as against those components involved in anchoring chromosomal structures, can reveal changes in chromosomal segregation.
Bayani
Jane
J
Princess Margaret Hospital and The Ontario Cancer Institute, Toronto, Ontario, Canada.
Squire
Jeremy A
JA
D018929:Cell Culture Techniques; D018797:Cell Cycle Proteins / Q000032:analysis / Q000648:ultrastructure; D002869:Chromosome Aberrations; D003584:Cytological Techniques; D007150:Immunohistochemistry; D008938:Mitosis; D008940:Mitotic Index; D016707:Tissue Fixation
D018797:Cell Cycle Proteins