Proteomic analysis of human amniotic fluid from Rh(-) pregnancy.
BACKGROUND
The objective of this study was to identify proteins from the amniotic fluid of the most common reproductive ailment, that is, Rh(-) incompatibility. In the present study, AF from Rh(-) pregnancy in the 29-35th week of gestation was analyzed by 2-DE, and our purpose was to identify the major spots on 2D gel.


METHODS
Proteins from an amniotic fluid sample was separated on 2D gel and visualized after silver staining. Most of the spots were analyzed by MALDI-TOF/MS, and were identified based on the NCBI database by a peptide mass fingerprinting method.


RESULTS
Albumin, serotransferrin, haptoglobin, alpha-fetoprotein and immunoglobulin were the major proteins detected. On comparison with 2D gel of normal AF, differences were observed at the level of albumin, and newly identified Calgranulins and Neutophil-defensins were observed. Many enzymes, growth factor binding proteins, protease inhibitors and proteins related to inflammation have also been identified.


CONCLUSIONS
This study demonstrates, for the first time, the potential utility of proteomics-based approaches to identify specific biomarkers like Calgranulins and Neutophil-defensins and diagnostic profiles for Rh(-) incompatibility processes and pathophysiologic conditions of pregnancy.
Hassan
Md Imtaiyaz
MI
Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
Kumar
Vijay
V
Singh
Tej P
TP
Yadav
Savita
S
D000418:Albumins / Q000032:analysis; D000653:Amniotic Fluid / Q000737:chemistry*; D015415:Biomarkers / Q000032:analysis; D001787:Blood Group Incompatibility / Q000503:physiopathology*; D023082:Defensins / Q000032:analysis; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D039841:Leukocyte L1 Antigen Complex / Q000032:analysis; D011247:Pregnancy; D011248:Pregnancy Complications / Q000503:physiopathology*; D011296:Prenatal Diagnosis; D040901:Proteomics; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000418:Albumins; D015415:Biomarkers; D023082:Defensins; D039841:Leukocyte L1 Antigen Complex; D012204:Rh-Hr Blood-Group System