title: BART is essential for nuclear retention of STAT3.
abstract: Signal transducers and activators of transcription (STATs) mediate cell proliferation, differentiation and survival in immune responses, hematopoiesis, neurogenesis and other biological processes. STAT3, for example, is involved in the epithelial-mesenchymal transition during gastrulation, organogenesis, wound healing and cancer progression. STAT activity is regulated by a variety of mechanisms, including nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT activity, we performed yeast two-hybrid screening. Here, we identified binder of ADP-ribosylation factor-like two (BART) as a novel STAT-binding partner. Importantly, we showed that BART is essential for the transcriptional activity and nuclear retention of STAT3. Furthermore, an effector of BART, ADP-ribosylation factor-like 2 (ARL2) was also involved in nuclear retention of STAT3. These results indicate that BART plays an essential role in the nuclear retention of STAT3 through interaction with ARL2.
authors.affiliation: Department of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-Ku Kita 12 Nishi 6, Sapporo, Hokkaido 060-0812, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Muromoto
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Sekine
authors.forename: Seiyu
authors.initials: S
authors.lastname: Imoto
authors.forename: Osamu
authors.initials: O
authors.lastname: Ikeda
authors.forename: Taichiro
authors.initials: T
authors.lastname: Okayama
authors.forename: Noriko
authors.initials: N
authors.lastname: Sato
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; C485819:Arl2 protein, mouse; D019204:GTP-Binding Proteins