title: A novel method of prediction and optimization for preparative high-performance liquid chromatography separation.
abstract: The preparation of components from a complex sample is a difficult task. The optimization of the separation and subsequent scale-up is usually carried out by trial and error. In this study, the relationship between retention parameters a and c for analytical and preparative separations was developed when the same solid adsorbent and mobile phase were used. The prediction and optimization of the preparative separation of a complex sample could be achieved by direct and simple conversion of the experimentally determined data from an analytical level. The novel method was successfully applied to optimize the separation of target compounds in traditional Chinese medicine (TCM).
authors.affiliation: Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Xiao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D008962:Models, Theoretical; D013816:Thermodynamics
chemical_list: D004365:Drugs, Chinese Herbal