[Biological effect of (60)Cogamma ray on alveolar type II cells and interstitial cells of alveoliar septum in radiation pulmonary injury].
OBJECTIVE
To explore the biological effect of (60)Cogamma ray on alveolar type II cells and interstitial cells of alveoliar septum.


METHODS
Alveolar type II cells(AT-II) and interstitial cells including interstitial macrophages and fibroblasts were irradiated by 0, 3, 5, 7 Gy of gamma ray respectively. The effect of irradiation on AT-II proliferation was observed by argentation against nucleus. The activity of MMP-2, -9 in supernatants from AT-II and interstitial cells after irradiation was determined by zymography. The levels of TGF-beta1 and collagen type IV in supernatant from interstitial cells after irradiation were measured by ELISA.


RESULTS
The nucleolus number of AT-II was increased with the increase of irradiation dose and group 7 Gy reached the highest level. The activity of MMP-2, -9 in supernatant from AT-II after irradiation increased at first and then decreased gradually. The activity of MMP-2, -9 and the content of TGF-beta1 in interstitial cells increased step by step, but collagen type IV decreased at first and then increased.


CONCLUSIONS
AT-II, macrophages and fibroblasts are all involved in pulmonary invalid remodeling course in early radiation pulmonary injury, which is related to the initiation of pulmonary fibrosis in late period.
Diao
Rui-ying
RY
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China. diaodiao005@163.com
Song
Liang-wen
LW
Wang
Shao-xia
SX
Li
Ming
M
D056809:Alveolar Epithelial Cells / Q000378:metabolism* / Q000528:radiation effects*; D000818:Animals; D002478:Cells, Cultured; D003037:Cobalt Radioisotopes / Q000009:adverse effects*; D024141:Collagen Type IV / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D055370:Lung Injury / Q000378:metabolism / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D003037:Cobalt Radioisotopes; D024141:Collagen Type IV; D053773:Transforming Growth Factor beta1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9