Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liquid chromatography.
Two novel methods for the analysis of ellagic acid in pomegranate (Punica granatum) rinds are proposed. Capillary electrophoresis (CE) was performed in a bare fused-silica capillary using a buffer solution of tri(hydroxymethyl)aminomethane:potassium dihydrogen phosphate (pH 8.4) with an applied voltage of 20 kV and UV detection at 254 nm. HPLC analysis was performed with a Zobax SB C(18) column and a mobile phase consisting of methanol:ethyl acetate:potassium dihydrogen phosphate: phosphoric acid at a flow rate of 1.0 mL/min. Under optimised conditions, the HPLC retention and the CE migration times for ellagic acid were 10.32 and 12.23 min, respectively. Calibration curves of peak area vs. concentration gave correlation coefficients of 0.9999 for HPLC and 0.9990 for CE. The detection limits for HPLC and CE were 2.8 and 2.2 microg/mL, respectively. Average recoveries were 98.32 +/- 1.2% for HPLC and 96.52 +/- 2.8% for CE. Both methods were shown to be suitable for the determination of ellagic acid in pomegranate rinds extraction; however, the CE method required less solvent and gave better column efficiency, whilst the HPLC provided superior precision.
Zhou
Benhong
B
Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuhan City, Hubei Province, People's Republic of China. benhongz@yahoo.com.cn
Wu
Zhenhua
Z
Li
Xiaojun
X
Zhang
Jie
J
Hu
Xianming
X
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004610:Ellagic Acid / Q000032:analysis*; D029561:Lythraceae / Q000737:chemistry*; D015203:Reproducibility of Results
D004610:Ellagic Acid