The preparation and characterization of poly(o-phenylenediamine)/gold nanoparticles interface for immunoassay by surface plasmon resonance and electrochemistry.
The poly-o-phenylenediamine (PoPD) nonconducting film and gold nanoparticles (AuNPs) were combined to fabricate AuNPs/PoPD film, which is used as a novel biocompatible interface for the immobilization of antibody and develop a simple and sensitive label-free immunoassay for the detection of the related antigen (human immunoglobulin G (IgG)). Surface plasmon resonance (SPR) and electrochemical methods were used to provide the real-time information about the polymer film growth, assembling of various sizes of gold nanoparticles, anti-human IgG antibody (anti-hIgG) immobilization and the antigen-antibody interaction. The microstructures of the PoPD and AuNPs/PoPD films were characterized by atomic force microscopy (AFM). These results demonstrated that AuNPs were uniformly dispersed on the porous surface of PoPD film, which formed a nano-structure biocompatible AuNPs/PoPD interface. The use of gold nanoparticles and PoPD film could enhance the immunoassay sensitivity and anti-nonspecific property of the resulting immunoassay electrode. Additionally, the reproducibility and preliminary application of anti-hIgG/AuNPs/PoPD/Au electrode for SPR detection of hIgG was also evaluated.
Wang
Qiong
Q
Ministry of Education Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Tang
Hao
H
Xie
Qingji
Q
Jia
Xueen
X
Zhang
Youyu
Y
Tan
Liang
L
Yao
Shouzhuo
S
D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D053758:Nanoparticles; D010655:Phenylenediamines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
D010655:Phenylenediamines; D011108:Polymers; D006046:Gold; C034193:1,2-diaminobenzene