title: The nucleocapsid protein of SARS-associated coronavirus inhibits B23 phosphorylation.
abstract: Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is responsible for SARS infection. Nucleocapsid (N) protein of SARS-CoV encapsidates the viral RNA and plays an important role in virus particle assembly and release. In this study, the N protein of SARS-CoV was found to associate with B23, a phosphoprotein in nucleolus, in vitro and in vivo. Mapping studies localized the critical N sequences for this interaction to amino acid residues 175-210, which included a serine/arginine (SR)-rich domain. In vitro phosphorylation assay showed that the N protein inhibited the B23 phosphorylation at Thr199.
authors.affiliation: State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan 430072, PR China.
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Linbai
authors.initials: L
authors.lastname: Ye
authors.forename: Shengli
authors.initials: S
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Weijia
authors.initials: W
authors.lastname: Cai
authors.forename: Liya
authors.initials: L
authors.lastname: Su
authors.forename: Yinglong
authors.initials: Y
authors.lastname: She
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Wu
mesh_terms: D001665:Binding Sites; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D019590:Nucleocapsid Proteins / Q000737:chemistry* / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; D019590:Nucleocapsid Proteins