title: Phosphate availability affects the tonoplast localization of PLDzeta2, an Arabidopsis thaliana phospholipase D.
abstract: Under phosphate deprivation, higher plants change their lipid composition and recycle phosphate from phospholipids. A phospholipase D, PLDzeta2, is involved in this recycling and in other cellular functions related to plant development. We investigated the localization of Arabidopsis PLDzeta2 by cell fractionation and in vivo GFP confocal imaging. AtPLDzeta2 localizes to the tonoplast and the Nter regulatory domain is sufficient for its sorting. Under phosphate deprivation, AtPLDzeta2 remains located in the tonoplast but its distribution is uneven. We observed PLDzeta2-enriched tonoplast domains preferentially positioned close to mitochondria and beside chloroplasts. In absence of PLDzeta2, membrane developments were visualized inside vacuoles.
authors.affiliation: Laboratoire de Physiologie Cellulaire Végétale, CNRS, CEA, INRA, Université J. Fourier, CEA-Grenoble, iRTSV/LPCV, 17 Rue des Martyrs, Grenoble, France.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaryo
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Dubots
authors.forename: Catherine
authors.initials: C
authors.lastname: Albrieux
authors.forename: Barbara
authors.initials: B
authors.lastname: Baldan
authors.forename: Maryse A
authors.initials: MA
authors.lastname: Block
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000378:metabolism*; D019470:Biolistics; D015153:Blotting, Western; D018548:Cotyledon / Q000201:enzymology / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007425:Intracellular Membranes / Q000201:enzymology; D018613:Microscopy, Confocal; D009154:Mutation / Q000235:genetics; D018532:Pisum sativum / Q000166:cytology; D010710:Phosphates / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D019441:Plant Epidermis / Q000166:cytology / Q000201:enzymology; D054046:Plant Stomata / Q000166:cytology; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014617:Vacuoles / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D029681:Arabidopsis Proteins; D010710:Phosphates; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D010739:Phospholipase D; C510354:phospholipase Dzeta2, Arabidopsis