[Analysis of pde6b mRNA sequences of Kunming mice and C57BL/6J mice].
OBJECTIVE
To clone and sequence the phosphodiesterase 6 beta subunit (pde6b) gene of Kunming mice, and to compare it with counterpart sequences in the GenBank database.


METHODS
The primers were designed covering the CDS region of the pde6b gene, and the corresponding fragments were amplified using RT-PCR. The fragments were cloned into plasmids, amplified in E.coli, and sequenced. Bioinformatics programs and online tools were used to analyze the sequences.


RESULTS
The CDS of the pde6b gene of Kunming mice was cloned and sequenced. Compared with the inbred mice C57BL/6J, pde6b CDS sequence of Kunming mice was different in some points: a G-->A transition at +706, a C-->T transversion at +1149. The protein sequence was of little difference: only a glycine-->serine at +236.


CONCLUSIONS
The pde6b CDS region of Kunming mice was successfully cloned. Pde6b sequences are of some level difference in different kinds of mice, but they are most conserved in protein level.
Yan
Guo-lin
GL
Department of Clinical Aerospace Medicine Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.
Wu
You-sheng
YS
Guo
Qun
Q
An
Jing
J
Liu
Xin-ping
XP
Zhang
Zuo-ming
ZM
D000818:Animals; D001483:Base Sequence; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000235:genetics*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D021122:Protein Subunits / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
D021122:Protein Subunits; D012333:RNA, Messenger; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6