[Effect of berberine on insulin secretion and glucokinase activity of NIT-1 cells].
To investigate the effect of berberine on insulin secretion of NIT-1 cells stimulated by glucose and the possible molecular mechanism, we used radioimmunoassay, scintillation counting technique, enzymatic method and Western blotting to measure the effects of berberine on insulin secretion, glucose utilization, the activity of glucokinase (GK) and protein level of GK and GK regulation protein (GKRP). Compared with untreated group, insulin secretion level, glucose utilization, the activity and protein level of GK in NIT-1 cells stimulated by high concentration of glucose were increased significantly in berberine group (P < 0.05), while the protein level of GKRP in berberine group decreased markedly. In conclusion, berberine can promote insulin secretion of NIT-1 cells induced by high concentration of glucose. The possible molecular mechanism may be associated with berberine acting as a GK activator, improving glucose utilization, enhancing the activity and protein expression level of GK, as well as decreased the protein level of GKRP.
Wang
Zeng-si
ZS
Institute of Integrative Traditional and Western Medicine, Tongli Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Lu
Fu-er
FE
Chen
Guang
G
Xu
Li-jun
LJ
Wang
Kai-fu
KF
Zou
Xin
X
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D002460:Cell Line; D005941:Glucokinase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D051379:Mice
D048868:Adaptor Proteins, Signal Transducing; C485100:GCKR protein, human; D007328:Insulin; D001599:Berberine; D005941:Glucokinase; D005947:Glucose