title: Expression of an activated form of integrin beta2 chain CD18 in cardiac surgical operations.
abstract: BACKGROUND
  Myeloid cells are extensively activated in patients undergoing cardiosurgical operations. It is supposed that this activation is more profound in patients operated with cardiopulmonary bypass (CPB) ("on-pump") in comparison with patients operated without CPB ("off-pump").
  
  
  OBJECTIVE
  To evaluate changes in the expression of a novel activation marker expressed on myeloid cells recognized by MEM-148 antibody.
  
  
  METHODS
  The expression of MEM-148 positive myeloid cells was evaluated by flow cytometry in 40 patients who underwent coronary artery bypass surgery (CABG) randomly assigned to "on-pump" or "off-pump" technique.
  
  
  RESULTS
  The relative and absolute number of MEM-148 positive myeloid cells is significantly diminished during "on-pump" surgery. A significant increase in their number in postoperative period in both "on-pump" and "off-pump" patients was found. There were no significant differencies between "on-pump" and "off-pump" patients.
  
  
  CONCLUSIONS
  The very trauma of surgery seems to be more relevant in starting on activation of myeloid cells them CPB itself.
authors.affiliation: Charles University in Prague, Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Department of Clinical Immunology and Allergology, Czech Republic.
authors.forename: Manuela
authors.initials: M
authors.lastname: Kudlová
authors.forename: Martina
authors.initials: M
authors.lastname: Kolácková
authors.forename: Pavel
authors.initials: P
authors.lastname: Kunes
authors.forename: Vladimír
authors.initials: V
authors.lastname: Lonský
authors.forename: Jirí
authors.initials: J
authors.lastname: Mand'ák
authors.forename: Andrýs
authors.initials: A
authors.lastname: Ctirad
authors.forename: Jan
authors.initials: J
authors.lastname: Krejsek
mesh_terms: D000368:Aged; D018821:CD18 Antigens / Q000097:blood*; D002315:Cardiopulmonary Bypass; D001026:Coronary Artery Bypass; D047549:Coronary Artery Bypass, Off-Pump; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008297:Male
chemical_list: D018821:CD18 Antigens