Multilaboratory testing of antifungal combinations against a quality control isolate of Candida krusei.
Candida krusei ATCC 6258 was tested by eight laboratories using 96-well plates containing checkerboard pairwise combinations of amphotericin B (AMB), posaconazole (PSC), caspofungin (CSP), and voriconazole (VRC). The methodology led to reproducible results across the laboratories. All drug combinations yielded MICs lower than the MICs of any two drugs tested singly, and combinations of AMB, PSC, CSP, and VRC were indifferent (no antagonism) by summations of fractional inhibitory concentration.
Chaturvedi
Vishnu
V
Mycology Laboratory, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208, USA. vishnu@wadsworth.org
Ramani
Rama
R
Ghannoum
Mahmoud A
MA
Killian
Scott B
SB
Holliday
Nicole
N
Knapp
Cindy
C
Ostrosky-Zeichner
Luis
L
Messer
Shawn A
SA
Pfaller
Michael A
MA
Iqbal
Naureen J
NJ
Arthington-Skaggs
Beth A
BA
Vazquez
Jose A
JA
Sein
Tin
T
Rex
John H
JH
Walsh
Thomas J
TJ
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D000077336:Caspofungin; D004359:Drug Therapy, Combination; D054714:Echinocandins / Q000494:pharmacology; D006801:Humans; D007753:Laboratories; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D011743:Pyrimidines / Q000494:pharmacology; D011786:Quality Control; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D011743:Pyrimidines; D014230:Triazoles; C101425:posaconazole; D000666:Amphotericin B; D000077336:Caspofungin; D065819:Voriconazole