Interaction trap/two-hybrid system to identify interacting proteins.
This unit presents protocols designed to detect interacting proteins. Using yeast as a "test tube" and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.
Golemis
E A
EA
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
Serebriiskii
I
I
Finley
R L
RL
Kolonin
M G
MG
Gyuris
J
J
Brent
R
R
D000818:Animals; D015723:Gene Library; D006801:Humans; D010957:Plasmids; D011506:Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
D011506:Proteins