Clinical uses of anti-Müllerian hormone assays: pitfalls and promises.
OBJECTIVE
To investigate whether the controversy about fluctuations of anti-Müllerian hormone (AMH) levels during the menstrual cycle results from differences between the immunoassays currently available: the Beckman Coulter Immunotech kit (Fullerton, CA) and the Diagnostic Systems Laboratories kit (Webster, TX).


METHODS
Prospective trial.


METHODS
Fertility clinics of two tertiary university hospitals.


METHODS
One hundred sixty-eight blood samples from three different populations. Serial samples at set intervals from the LH surge were taken in a fourth population of 10 volunteers.


METHODS
We remeasured AMH levels by using the Diagnostic Systems Laboratories kit in 168 blood samples in which AMH initially had been measured by using the Beckman Coulter assay. We also conducted serial AMH measurements (n = 7) during the menstrual cycle of 10 women.


METHODS
Linear regression of AMH levels determined by using 2 different assays and analysis of variance of serial measurements in the menstrual cycle.


RESULTS
We found a linear relationship between the 2 methods, with a correlation coefficient of 0.88. When repeated individual AMH measures were longitudinally analyzed in relation to the LH surge, a slight but significant decrease was observed after ovulation.


CONCLUSIONS
Differences in AMH fluctuations during the menstrual cycle reported in recent publications do not result from the use of different AMH assays. The changes in AMH levels after ovulation are slight, yet statistically significant. However, the fluctuations observed are smaller than intercycle variability and therefore are not clinically relevant as far as AMH measurements for clinical purposes are concerned. In daily practice, AMH therefore can be measured anytime during the menstrual cycle.
Streuli
Isabelle
I
Joint Division of Reproductive Endocrinology and Infertility, University Hospitals of Geneva and Lausanne, Geneva, Switzerland.
Fraisse
Timothee
T
Chapron
Charles
C
Bijaoui
Gérard
G
Bischof
Paul
P
de Ziegler
Dominique
D
D000328:Adult; D054304:Anti-Mullerian Hormone / Q000097:blood*; D005260:Female; D006801:Humans; D007118:Immunoassay; D008137:Longitudinal Studies; D008597:Menstrual Cycle / Q000502:physiology*; D010060:Ovulation / Q000502:physiology; D011446:Prospective Studies; D012016:Reference Values; D012044:Regression Analysis; D015203:Reproducibility of Results
D054304:Anti-Mullerian Hormone