title: Fluorescent staining of subcellular organelles: ER, Golgi complex, and mitochondria.
abstract: The ability to distinguish and identify specific subcellular compartments is essential to understanding organelle function, biogenesis, and maintenance within cells and to defining protein trafficking pathways. Fluorescent dyes and/or fluorescently labeled lipid derivatives can be used to identify ER, Golgi complex, and mitochondria. Specific conditions for labeling each of these compartments are described.
authors.affiliation: University of Connecticut Health Center, Farmington, Connecticut, USA.
authors.forename: M
authors.initials: M
authors.lastname: Terasaki
authors.forename: L
authors.initials: L
authors.lastname: Loew
authors.forename: J
authors.initials: J
authors.lastname: Lippincott-Schwartz
authors.forename: K
authors.initials: K
authors.lastname: Zaal
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D000818:Animals; D002232:Carbocyanines / Q000032:analysis / Q000737:chemistry; D002478:Cells, Cultured / Q000648:ultrastructure; D002518:Ceramides / Q000032:analysis / Q000737:chemistry; D004396:Coloring Agents / Q000032:analysis / Q000737:chemistry; D004721:Endoplasmic Reticulum / Q000737:chemistry / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006056:Golgi Apparatus / Q000737:chemistry / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000737:chemistry / Q000648:ultrastructure; D008928:Mitochondria / Q000737:chemistry / Q000648:ultrastructure*; D012235:Rhodamines / Q000032:analysis / Q000737:chemistry; D013048:Specimen Handling / Q000379:methods; D013110:Sphingosine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D002232:Carbocyanines; D002518:Ceramides; D004396:Coloring Agents; D005456:Fluorescent Dyes; C527864:N-(5-(5,7-dimethyl Bodipy)-1-pentanoyl)-erythrosphingosine; D012235:Rhodamines; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; C005358:tetramethylrhodamine; C037974:N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl sphingosine; D009327:4-Chloro-7-nitrobenzofurazan; D013110:Sphingosine