Assessment of bovine herpesvirus 4 based vector in chicken.
The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the ability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, such as sheep, goats, swine, cats, dogs, rabbits, mink, horses, turkeys, ferrets, monkeys, hamsters, rats, mice, and chickens. In this report, the feasibility to use BoHV-4 based vector in chicken was investigated. Although BoHV-4 was able to replicate, leading to a cytopathic effect in a chicken cell line and infect the chorion allantoic membrane of embryonated eggs, however it was not pathogenic even when a large dose of virus was injected into the chicken. An immune response could be produced against heterologous antigen delivered by a recombinant BoHV-4. These data suggest the feasibility of using BoHV-4 based vector for vaccination purposes in chickens.
Donofrio
Gaetano
G
Dipartimento di Salute Animale, Sezione di Malattie Infettive degli Animali, Facoltà di Medicina Veterinaria, 43100 Parma, Italy. gaetano.donofrio@unipr.it <gaetano.donofrio@unipr.it>
Manarolla
Giovanni
G
Ravanetti
Lara
L
Sironi
Giuseppe
G
Cavirani
Sandro
S
Cabassi
Clotilde Silvia
CS
Flammini
Cesidio Filippo
CF
Rampin
Tiziana
T
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D005822:Genetic Vectors; D026021:Herpesvirus 4, Bovine / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D014611:Vaccination / Q000379:methods*; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology
D014765:Viral Vaccines