High-performance liquid chromatography spectrometric analysis of tripterin in rat plasma.
A quick, precise and reliable HPLC method has been developed to determine tripterin in rat plasma. After liquid-liquid extraction, the analytes was analyzed on a Discovery ODS C(18) column (5microm, 4.6mmx250mm) with an isocratic elution consisting of methanol-water-phosphoric acid (87:13:0.2, v/v/v). Ultraviolet detection was at 425nm. Using trioxymethylanthraquinone as an internal standard, the assay was linear over the concentration range of 0.025-1.60microg/mL (r(2)=0.9988). The extraction recovery of tripterin in rat plasma was more than 62%. The intra- and inter-day precision was less than 13% (CV). This validated method was successfully applied to the pharmacokinetics of tripterin in rats.
Wang
Wenyan
W
School of Pharmacy, Yantai University, Yantai 264005, China.
Liu
Ke
K
Dong
Hongwei
H
Liu
Wanhui
W
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D007275:Injections, Intravenous; D053978:Pentacyclic Triterpenes; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014315:Triterpenes / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics
D000975:Antioxidants; D007202:Indicators and Reagents; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; C050414:celastrol