Biosynthesis of [1-15N] L-tryptophan from 15N labeled anthranilic acid by fermentation of Candida utilis mutant.
A new method for synthesizing the labeled L-tryptophan is described in this work. L-tryptophan, labeled with 98% (15)N at position 1 was synthesized from the labeled anthranilic acid using Candida utilis mutants. The conversion ratio of (15)N of 50% was achieved. The labeled anthranilic acid was synthesized by [(15)N] phthalimide that was prepared by 99.34% [(15)N] urea and phthalic anhydride in ortho-xylene medium at 140 degrees C and under atmospheric pressure.
Liu
Zhanfeng
Z
College of Life Science and Technology, Beijing University of Chemical Technology, P. O. Box 75, 100029, Beijing, People's Republic of China.
Yuan
Qipeng
Q
Wang
Wenchuan
W
D002175:Candida / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D009587:Nitrogen Isotopes; D014364:Tryptophan / Q000096:biosynthesis* / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry / Q000378:metabolism*
D009587:Nitrogen Isotopes; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D014364:Tryptophan