title: Alkaline phosphatase, glutathione-S-transferase-P, and cofilin-1 distinguish multipotent mesenchymal stromal cell lines derived from the bone marrow versus peripheral blood.
abstract: Multipotent mesenchymal stromal cells (MSCs) can be isolated from bone marrow or peripheral blood. To identify phenotypical and functional differences between MSCs derived from these sources, the human bone marrow-derived, fibroblast-like cell line L87/4 was compared with the peripheral blood-derived, fibroblast-like cell line V54/2. Both cell lines expressed similar levels of SH3+, CD45(-), CD68(-), CD133(-), and HLA-DR(-). The bone marrow-derived cells expressed higher surface levels of CD105, CD10, and CD117 and preferentially expressed alkaline phosphatase, glutathione S-transferase P, and cofilin-1. The peripheral blood-derived line showed a higher number of CD34+/CD105+ double-positive and side population (SP) cells. The results demonstrate the more multipotent, yet quiescent, stromal phenotype of bone marrow MSCs, whereas MSCs isolated from the circulation display more hematopoietic-lineage characteristics. Importantly, potential marker genes that distinguish the two stages of MSCs are defined.
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA. cconrad1@partners.org
authors.forename: Claudius
authors.initials: C
authors.lastname: Conrad
authors.forename: Evelyn
authors.initials: E
authors.lastname: Zeindl-Eberhart
authors.forename: Sabine
authors.initials: S
authors.lastname: Moosmann
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Nelson
authors.forename: Christiane J
authors.initials: CJ
authors.lastname: Bruns
authors.forename: Ralf
authors.initials: R
authors.lastname: Huss
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D001773:Blood Cells; D001854:Bone Marrow Cells; D002460:Cell Line; D051338:Cofilin 1 / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D059630:Mesenchymal Stem Cells; D039902:Multipotent Stem Cells; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D015415:Biomarkers; D051338:Cofilin 1; D005982:Glutathione Transferase; D000469:Alkaline Phosphatase