Direct evidence of mast cell participation in acute acid-induced esophageal inflammation in mice.
OBJECTIVE
Mast cells have been implicated in the pathogenesis of esophagitis resulting from gastroesophageal acid reflux, but their precise role has been difficult to define. We proposed to directly examine the contribution of mast cells to neutrophil infiltration in a mouse model of acid-induced esophageal injury.


METHODS
Normal and mast cell-deficient (Kit) mice underwent either a surgical procedure to induce acute acid reflux injury of the esophagus or sham surgery. Neutrophil infiltration in the esophagus was determined by morphometrical quantification. To further delineate the involvement of mast cells, acid-induced esophageal injury was elicited in mast cell-deficient mice that had undergone mast cell reconstitution by bone marrow transplantation.


RESULTS
Normal mice exhibited significant neutrophil infiltration into the esophagus as a result of acid-induced injury. The neutrophil accumulation was significantly diminished in mast cell-deficient mice. However, the neutrophil infiltration that resulted from acid-induced injury in mast cell-reconstituted Kit mice was similar to that seen in normal mice.


CONCLUSIONS
Our findings provide direct evidence that mast cells participate in the recruitment of neutrophils during acid-induced esophageal injury in mice.
Morganstern
Jeffrey A
JA
The Children's Hospital at Montefiore and Albert Einstein College of Medicine, Bronx, NY 10467, USA.
Wang
Ming-Yu
MY
Wershil
Barry K
BK
D000818:Animals; D004195:Disease Models, Animal; D004942:Esophagitis, Peptic / Q000276:immunology* / Q000473:pathology*; D019317:Evidence-Based Medicine; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D018375:Neutrophil Activation; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology*