Biomarker responses in caged rockfish (Sebastes schlegeli) from Masan Bay and Haegeumgang, South Korea.
The purpose of this study was to compare enzymatic biomarker activities in fish caged at two sites, Masan Bay (contaminated) and Haeguemgang (reference). In the present study, ethoxyresorufin O-deethylase (EROD), brain acetyl cholinesterase (bAChE), muscle acetyl cholinesterase (mAChE) and butyryl cholinesterase (mBChE) in caged rockfish (Sebastes schlegeli) were measured 0, 1, 3, 7, 14, 21 and 30 days after caging. The level of CYP1A mRNA and Protein expression was induced higher in Masan Bay at 1, 3, 7, 14 and 30 days after caging. EROD activity in the caged fish was significantly higher in Masan Bay than in Haeguemgang 3 and 7 days after caging, but not at 14 and 30 days after caging. bAChE activity was significantly inhibited at 7 and 14 days after caging in Masan Bay. However, mBChE activity was not significantly inhibited during the experiment. Taken together, the data suggest that the caged fish were exposed, at least transiently, to CYP1A inducers and ChE inhibitors, which is consistent with our previous observations.
Jung
Jee-Hyun
JH
South Sea Institute, Korean Ocean Research and Development Institute (KORDI), 391 Jangbuk-Ri Jangmonk-myon, Geoje 656-830, Republic of Korea.
Kim
So-Jueng
SJ
Lee
Taek-Kyun
TK
Shim
Won Joon
WJ
Woo
Seonock
S
Kim
Dae-Jung
DJ
Han
Chang-Hee
CH
D000110:Acetylcholinesterase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D019363:Cytochrome P-450 CYP1A1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005398:Fisheries / Q000295:instrumentation; D005399:Fishes / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007723:Korea; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment
D015415:Biomarkers; D012333:RNA, Messenger; D019363:Cytochrome P-450 CYP1A1; D000110:Acetylcholinesterase