Amyloid fibril formation and chaperone-like activity of peptides from alphaA-crystallin.
AlphaA-crystallin (alphaAC), a major component of eye lens, exhibits chaperone-like activity and is responsible for maintaining eye lens transparency. Synthetic peptides which corresponded to the putative substrate-binding site of alphaAC have been reported to prevent aggregation of proteins [Sharma, K. K., et al. (2000) J. Biol. Chem. 275, 3767-3771]. In this study, we found that these peptides, alphaAC(70-88), the peptide corresponding to amino acids 70-88 of alphaAC (KFVIFLDVKHFSPEDLTVK), and alphaAC(71-88), suppressed the amyloid fibril formation of amyloid beta protein (Abeta). On the other hand, while alphaAC(71-88) exhibited chaperone-like activity toward insulin, alphaAC(70-88) and alphaAC(70-88)K70D promoted rapid growth of aggregates consisting of insulin and these peptides in their solution mixtures. Interestingly, we found that alphaAC(71-88) itself can also form amyloid fibrils. It is possible that the chaperone-like activity of the alphaAC peptides is potentially related to their propensity for amyloid fibril formation. Analysis of variants of the alphaAC peptides suggested that F71 is important for amyloid formation, and interestingly, this same residue has previously been found to be essential for chaperone-like activity. Amyloid fibril formation was also observed with the shorter peptide, alphaAC(70-76)K70D, showing that the ability to form amyloid fibrils is maintained even with significant deletion of the C-terminal sequence. The formation of amyloid fibril was suppressed in alphaAC(70-88), suggesting that the K70 in the substrate binding site may play a role in suppressing the amyloid fibril formation of alphaAC, which agreed with recent proposals about the presence of an aggregation suppressor in the region flanking aggregation-prone hydrophobic sequences.
Tanaka
Naoki
N
Department of Bio-molecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan. tanaka@kit.ac.jp
Tanaka
Ryoji
R
Tokuhara
Mutsumi
M
Kunugi
Shigeru
S
Lee
Yin-Fai
YF
Hamada
Daizo
D
D000682:Amyloid / Q000737:chemistry*; D002942:Circular Dichroism; D007328:Insulin / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010446:Peptide Fragments / Q000737:chemistry*; D011485:Protein Binding; D038202:alpha-Crystallin A Chain / Q000737:chemistry*
D000682:Amyloid; D007328:Insulin; D010446:Peptide Fragments; D038202:alpha-Crystallin A Chain