Molecular characterization of tree peony germplasm using sequence-related amplified polymorphism markers.
This study examined 63 tree peony specimens, consisting of 3 wild species and 63 cultivars, using sequence-related amplified polymorphism (SRAP) markers for the purpose of detecting genomic polymorphisms. Bulk DNA samples from each specimen were evaluated with 23 SRAP primer pairs. Among the 296 different amplicons, 262 were polymorphic. The maximum parsimony, neighbor-joining, and unweighted pair-group method using arithmetic average trees were largely in congruence. In the three trees, the wild species Paeonia ludlowii and P. delavayi formed separate clusters with strong bootstrap support, and P. ostii was closely related to all cultivars. The cultivars were divided into groups with various corresponding bootstrap values. The genetic similarity among the genotypes ranged from 0.02 to 0.73. These results demonstrate that SRAP markers are effective in detecting genomic polymorphisms in the tree peony and should be useful for linkage map construction and molecular marker assisted selection breeding.
Han
Xiao Yan
XY
Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxin Cun, Xiangshan, Haidian District, Beijing, 100093, P.R. China.
Wang
Liang Sheng
LS
Shu
Qing Yan
QY
Liu
Zheng An
ZA
Xu
Su Xia
SX
Tetsumura
Takuya
T
D001947:Breeding; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D029596:Paeonia / Q000145:classification / Q000166:cytology* / Q000235:genetics*; D011110:Polymorphism, Genetic
D018744:DNA, Plant; D005819:Genetic Markers