title: Detection of mitotic figures and components of the mitotic machinery.
abstract: Mitotic figures have provided important information on the mechanisms behind observed chromosomal aberrations. Variations in cell culturing and fixation methods enhance the visualization of mitotic figures. Furthermore, immunohistochemistry using a host of antibodies against protein structures involved in the cell cycle, as well as against those components involved in anchoring chromosomal structures, can reveal changes in chromosomal segregation.
authors.affiliation: Princess Margaret Hospital and The Ontario Cancer Institute, Toronto, Ontario, Canada.
authors.forename: Jane
authors.initials: J
authors.lastname: Bayani
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Squire
mesh_terms: D018929:Cell Culture Techniques; D018797:Cell Cycle Proteins / Q000032:analysis / Q000648:ultrastructure; D002869:Chromosome Aberrations; D003584:Cytological Techniques; D007150:Immunohistochemistry; D008938:Mitosis; D008940:Mitotic Index; D016707:Tissue Fixation
chemical_list: D018797:Cell Cycle Proteins