[Genetic variation in RXRG gene and its relationship with twinning trait in cattle].
Retinoic X receptor-gamma (RXRG) gene was studied as a candidate gene for the twinning trait of bovine. A new SNP A1941G was detected by sequencing at 3'UTR. Different genotypes were determined in Luxi monotocous cows, Luxi twinning cows, Chinese Simmental cows, Angus cows and Simmental x Mongolia cows by restriction fragment length polymorphism (RFLP). The value of polymorphism information content indicated that this was a moderate polymorphism in Luxi monotocous cows and Luxi twinning cows. The chi(2) test indicated that the polymorphic locus in Luxi twinning cows did not fit Hardy-Weinberg equilibrium (Plt;0.05). The chi(2) test of associational analysis between genotypic distribution and twinning or monotocous trait in Luxi cows showed that the difference was very significant (Plt;0.01).
Huang
Meng
M
College of Animal Science-technology, Northwest A &F University, Yangling 712100, China. huangmeng82@yahoo.com.cn
Xu
Shang-Zhong
SZ
Zan
Lin-Sen
LS
Zhang
Lu-Pei
LP
Gao
Xue
X
Chen
Jin-Bao
JB
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000502:physiology*; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D006579:Heterozygote; D016013:Likelihood Functions; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D047491:Retinoid X Receptor gamma / Q000235:genetics* / Q000378:metabolism; D014427:Twins / Q000235:genetics
D047491:Retinoid X Receptor gamma; D004247:DNA