Characteristic expression of twelve rice PR1 family genes in response to pathogen infection, wounding, and defense-related signal compounds (121/180).
Pathogenesis-related (PR) proteins have been used as markers of plant defense responses, and are classified into 17 families. However, precise information on the majority members in specific PR families is still limited. We were interested in the individual characteristics of rice PR1 family genes, and selected 12 putatively active genes using rice genome databases for expressed genes. All were upregulated upon compatible and/or incompatible rice-blast fungus interactions; three were upregulated in the early infection period and four in the late infection period. Upon compatible rice-bacterial blight interaction, four genes were upregulated, six were not affected, and one was downregulated. These results are in striking contrast to those among 22 Arabidopsis PR1 genes where only one gene was pathogen-inducible. The responses of individual genes to salicylic acid, jasmonic acid, and ethylene induced defense signaling pathways in rice are likely to be different from those in dicot plants. Transcript levels in healthy leaves, roots, and flowers varied according to each gene. Analysis of the partially overlapping expression patterns of rice PR1 genes in healthy tissues and in response to pathogens and other stresses would be useful to understand their possible functions and for use as characteristic markers for defense-related studies in rice.
Mitsuhara
Ichiro
I
National Institute of Agrobiological Sciences (NIAS), Kannon-dai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan. mituhara@affrc.go.jp
Iwai
Takayoshi
T
Seo
Shigemi
S
Yanagawa
Yuki
Y
Kawahigasi
Hiroyuki
H
Hirose
Sakino
S
Ohkawa
Yasunobu
Y
Ohashi
Yuko
Y
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D054884:Host-Pathogen Interactions / Q000235:genetics; D020082:Magnaporthe / Q000472:pathogenicity; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics; D014974:Xanthomonas / Q000472:pathogenicity
D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant