[Site-directed mutagenesis of sugar-binding lectin fragments of legume plants with the help of inverse-PCR].
Method of site-directed mutagenesis that gave an opportunity to the site-independent manipulation with enough extended exposed mutagenesis fragments of gene cloning in plasmid vector was developed. With the help of this method hybrid constructions representing peas Pisum sativum (PSL) lectine with carbohydrate-binding fragment changing on that lectines from another legume plants were created. This method is suitable for obtaining the expression constructions in plasmid vectors, when is necessary to put a coding part under the control of promoter in a certain reading frame.
Baĭĭmiev
Al Kh
AKh
Gubaĭdullin
I I
II
Baĭmiev
An Kh
AKh
Chemeris
A V
AV
D017343:Genes, Plant; D016297:Mutagenesis, Site-Directed; D018532:Pisum sativum / Q000235:genetics*; D037121:Plant Lectins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D037121:Plant Lectins; D011994:Recombinant Proteins