title: [Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene].
abstract: OBJECTIVE
  To construct the virus-like parcel expressing hepatitis B virus (HBV) C gene and identify its immunogenicity.
  
  
  METHODS
  HBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara (MVA).
  
  
  RESULTS
  pSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immunogenicity.
  
  
  CONCLUSIONS
  The MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
authors.affiliation: Postgraduate Medical School of PLA, Beijing 100853, China. luanxiangling@sohu.com
authors.forename: Xiang-ling
authors.initials: XL
authors.lastname: Luan
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.forename: Su-jun
authors.initials: SJ
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Lei
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Shen
authors.forename: Yi-chen
authors.initials: YC
authors.lastname: Wu
authors.forename: Shao-li
authors.initials: SL
authors.lastname: You
authors.forename: Pan-yong
authors.initials: PY
authors.lastname: Mao
authors.forename: Shao-jie
authors.initials: SJ
authors.lastname: Xin
mesh_terms: D005814:Genes, Viral; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000235:genetics*; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D006512:Hepatitis B Core Antigens