[Optimization of the polymerization conditions of vinblastine imprinted polymer by uniform design].
OBJECTIVE
To optimize the polymerization conditions of vinblastine (VLB) imprinted polymer.


METHODS
The conditions were optimized by the method of uniform design. The major factors investigated included the amount of functional monomer (MAA) and the cross-linker (EDMA) and the progenic solvent (toluene or acetonitril). The adsorption rate of VLB on the solid-phase extraction (SPE) column packed with MIP was adopted as the response value.


RESULTS
The optimal conditions were MAA 0.4 mmol, EDMA 1.6 mmol and using acetonitrile as the solvent. Under the conditions,the VLB imprinted polymer was synthesized and the absorption rate of VLB was 88.20%. The characterizations of the optimal MIP were determined by IR spectrometry and scanning electron microscope (SEM) analysis.


CONCLUSIONS
It is possible to furtherly improve the nature of the polymer by optimizing the polymerization parameters with uniform design. The polymer synthesized under the optimal conditions exhibited high affinity to the target molecule VLB.
Zhu
Quan-hong
QH
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. zqhyar@163.com
Feng
Jian-yong
JY
D000327:Adsorption; D003432:Cross-Linking Reagents / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D008689:Methacrylates / Q000008:administration & dosage / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods; D011108:Polymers / Q000737:chemistry* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D014747:Vinblastine / Q000737:chemistry* / Q000302:isolation & purification
D003432:Cross-Linking Reagents; D004365:Drugs, Chinese Herbal; D008689:Methacrylates; D011108:Polymers; D012997:Solvents; D014747:Vinblastine