A strategy for the covalent functionalization of resorbable polymers with heparin and osteoinductive growth factor.
The chemical strategy presented herein is the nondestructive preparation of resorbable polymer scaffolds with heparin covalently bonded to the surface and an osteoinductive growth factor, recombinant human bone morphogenetic protein-2, immobilized in the heparin layer. The coupling scheme involves functionalization of surfaces by grafting in the vapor phase with poly( l-lactide) and poly(-caprolactone) films chosen as representative substrates. The biocompatibility of functionalized surfaces was verified by a much improved attachment and proliferation of mesenchymal stem cells (MSC).
Edlund
Ulrica
U
Fibre and Polymer Technology, Royal Institute of Technology, SE-100 44 Stockholm, Sweden.
Dånmark
Staffan
S
Albertsson
Ann-Christine
AC
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry* / Q000494:pharmacology; D018786:Bone Substitutes; D002460:Cell Line; D049109:Cell Proliferation; D006493:Heparin / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D051379:Mice; D011091:Polyesters / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000494:pharmacology
C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D011091:Polyesters; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C016240:polycaprolactone; C033616:poly(lactide); D006493:Heparin