title: [Effect of TTRAP expression on apoptosis induced by hydroquinone in HL-60 cells in vitro.].
abstract: OBJECTIVE
  To study the effect of TTRAP expression on apoptosis induced by hydroquinone in HL-60 cells in vitro, and explore the relationship between TTRAP expression and the apoptosis.
  
  
  METHODS
  Apoptotic and necrotic rate was examined by flow cytometer with Anti-AnnexinV/FITC Plus PI staining. The mRNA expression of TTRAP was detected by RT-PCR. The differences in different treated groups were compared.
  
  
  RESULTS
  After different concentrations of hydroquinone to the cells for 0, 4, 8, 12 h culture, were added, the cell apoptotic rate in different concentrations of hydroquinone groups was significantly higher than that in blank control groups. The optimal concentration of hydroquinone was 200 micromol/L, lasting for 8 h. When it was 250 micromol/L, the necrotic rate increased significantly. The apoptosis induced by hydroquinone was associated with the culture time at the concentration of 200 micromol/L, and the peak apoptotic time was 8 h. Then the apoptotic rate decreased and necrotic rate increased. Furthermore, with the concentrations of hydroquinone increased and time lasted for 8 h, the apoptotic rate of cells increased, the amount of TTRAP expression in the mRNA level also increased accordingly. When the concentrations of hydroquinone was above 250 micromol/L, necrotic rate increased sharply, and the amount of TTRAP expression decreased.
  
  
  CONCLUSIONS
  Hydroquinone could induce apoptosis of HL-60 cells. The up-regulation of TTRAP expression may promote hydroquinone to induce HL-60 cells to go into apoptosis in vitro with dose-effect and time-effect relationship.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan 430071, China.
authors.forename: Zheng-Bin
authors.initials: ZB
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.forename: Yong-Yi
authors.initials: YY
authors.lastname: Bi
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhao
authors.forename: Ning
authors.initials: N
authors.lastname: Tao
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Zhong-Ling
authors.initials: ZL
authors.lastname: Zhu
authors.forename: Yang-Ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D006873:Hydroquinones