title: Determination of Akt/PKB signaling.
abstract: This unit provides a basic protocol for assaying the level of activity, as well as the activation capacity and dynamics of inhibition of Akt/PKB in cultured cells. All these data can be obtained in a single nonradioactive experiment by standard techniques including immunoblotting and immunodetection with phosphospecific antibodies. This unit also provides a support protocol for assaying the membrane translocation of Akt.
authors.affiliation: National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Roumen
authors.initials: R
authors.lastname: Pankov
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002470:Cell Survival; D002478:Cells, Cultured; D008722:Methods; D051379:Mice; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D051057:Proto-Oncogene Proteins c-akt