title: Dissociation from the oligomeric state is the rate-limiting step in fibril formation by kappa-casein.
abstract: Amyloid fibrils are aggregated and precipitated forms of protein in which the protein exists in highly ordered, long, unbranching threadlike formations that are stable and resistant to degradation by proteases. Fibril formation is an ordered process that typically involves the unfolding of a protein to partially folded states that subsequently interact and aggregate through a nucleation-dependent mechanism. Here we report on studies investigating the molecular basis of the inherent propensity of the milk protein, kappa-casein, to form amyloid fibrils. Using reduced and carboxymethylated kappa-casein (RCMkappa-CN), we show that fibril formation is accompanied by a characteristic increase in thioflavin T fluorescence intensity, solution turbidity, and beta-sheet content of the protein. However, the lag phase of RCMkappa-CN fibril formation is independent of protein concentration, and the rate of fibril formation does not increase upon the addition of seeds (preformed fibrils). Therefore, its mechanism of fibril formation differs from the archetypal nucleation-dependent aggregation mechanism. By digestion with trypsin or proteinase K and identification by mass spectrometry, we have determined that the region from Tyr(25) to Lys(86) is incorporated into the core of the fibrils. We suggest that this region, which is predicted to be aggregation-prone, accounts for the amyloidogenic nature of kappa-casein. Based on these data, we propose that fibril formation by RCMkappa-CN occurs through a novel mechanism whereby the rate-limiting step is the dissociation of an amyloidogenic precursor from an oligomeric state rather than the formation of stable nuclei, as has been described for most other fibril-forming systems.
authors.affiliation: School of Chemistry and the Physics, University of Adelaide, Adelaide, South Australia. heath.ecroyd@adelaide.edu.au
authors.forename: Heath
authors.initials: H
authors.lastname: Ecroyd
authors.forename: Tomas
authors.initials: T
authors.lastname: Koudelka
authors.forename: David C
authors.initials: DC
authors.lastname: Thorn
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Williams
authors.forename: Glyn
authors.initials: G
authors.lastname: Devlin
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
authors.forename: John A
authors.initials: JA
authors.lastname: Carver
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D052160:Benzothiazoles; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D019286:Endopeptidase K / Q000737:chemistry*; D013058:Mass Spectrometry; D008956:Models, Chemical; D009391:Nephelometry and Turbidimetry; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry*
chemical_list: D000682:Amyloid; D052160:Benzothiazoles; D002364:Caseins; D013844:Thiazoles; C009462:thioflavin T; D014357:Trypsin; D019286:Endopeptidase K