title: A recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken Mycoplasma gallisepticum infections.
abstract: Mycoplasma gallisepticum is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Serological monitoring is essential to maintain mycoplasma-free breeder flocks and often complicated by the cross-reactions between different mycoplasma species. To overcome serological cross-reactions, a large fragment of the M. gallisepticum PvpA cytadhesin, species-specific surface-exposed protein, was produced in E. coli as a recombinant protein (rPvpA336) and used as a potential diagnostic antigen. The rPvpA336 protein possesses 336 mycoplasma-specific amino acids with relative molecular weight of 44 kDa. A deletion region of 37 amino acids was identified when compared to the wild-type PvpA protein. Immunoreactivity of the rPvpA336 protein has been demonstrated by Western blot analysis with M. gallisepticum-positive and -negative chicken sera. Furthermore, an enzymatic rapid immunofiltration assay (ERIFA) prototype based on the rPvpA336 protein has been developed and its species-specific detection capability has been demonstrated by using M. gallisepticum and/or M. synoviae-positive and -negative chicken sera. In addition to its species-specificity, the ERIFA prototype presents certain advantages such as rapidity, field-applicability and cost-effectiveness. Therefore, these advantages would make the prototype a species-specific rapid diagnostic tool of choice in the field and limited laboratory conditions for screening M. gallisepticum infections.
authors.affiliation: Ondokuz Mayis University, Faculty of Veterinary Medicine, Department of Microbiology, 55139 Kurupelit, Samsun, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Büyüktanir
authors.forename: Tuba
authors.initials: T
authors.lastname: Yildirim
authors.forename: Cengiz
authors.initials: C
authors.lastname: Yakicier
authors.forename: Oktay
authors.initials: O
authors.lastname: Genç
authors.forename: Nevzat
authors.initials: N
authors.lastname: Yurdusev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D002645:Chickens; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D045686:Mycoplasma gallisepticum; D011201:Poultry Diseases / Q000175:diagnosis*; D011994:Recombinant Proteins
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins