Purification of immunoglobulin G.
While unpurified antibodies are suitable for a number of applications, purified antibodies are required for assays based on known concentration of antibody, for chemical modifications such as radiolabeling or conjugation with fluorochromes, or for structural modifications such as production of F(ab')2 or monvalent Fab fragments. This unit contains protocols for purification of IgG by ammonium sulfate precipitation coupled with size-exclusion chromatography, Protein A- and Protein G-affinity chromatography, immunoaffinity chromatography, and ion-exchange chromatography.
Andrew
S M
SM
Chester College, Chester, United Kingdom.
Titus
J A
JA
D000818:Animals; D002845:Chromatography / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007158:Immunologic Techniques
D007074:Immunoglobulin G