Nap1 links transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.
Chromatin remodeling is central to the regulation of transcription elongation. We demonstrate that the conserved Saccharomyces cerevisiae histone chaperone Nap1 associates with chromatin. We show that Nap1 regulates transcription of PHO5, and the increase in transcript level and the higher phosphatase activity plateau observed for Deltanap1 cells suggest that the net function of Nap1 is to facilitate nucleosome reassembly during transcription elongation. To further our understanding of histone chaperones in transcription elongation, we identified factors that regulate the function of Nap1 in this process. One factor investigated is an essential mRNA export and TREX complex component, Yra1. Nap1 interacts directly with Yra1 and genetically with other TREX complex components and the mRNA export factor Mex67. Additionally, we show that the recruitment of Nap1 to the coding region of actively transcribed genes is Yra1 dependent and that its recruitment to promoters is TREX complex independent. These observations suggest that Nap1 functions provide a new connection between transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.
Del Rosario
Brian C
BC
Center for Cell Signaling, University of Virginia, Charlottesville, VA 22908, USA.
Pemberton
Lucy F
LF
D000135:Acid Phosphatase; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D029543:Nucleocytoplasmic Transport Proteins / Q000502:physiology; D056490:Nucleosome Assembly Protein 1; D009707:Nucleosomes / Q000378:metabolism / Q000648:ultrastructure; D016366:Open Reading Frames; D025941:Protein Interaction Mapping; D012319:RNA Polymerase II / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism; D034443:RNA Transport / Q000235:genetics / Q000502:physiology*; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D012261:Ribonucleoproteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology*
D018797:Cell Cycle Proteins; C106898:MEX67 protein, S cerevisiae; D046912:Multiprotein Complexes; C533556:NAP1 protein, S cerevisiae; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D056490:Nucleosome Assembly Protein 1; D009707:Nucleosomes; D012322:RNA Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae; D012319:RNA Polymerase II; D000135:Acid Phosphatase; C488916:PHO5 protein, S cerevisiae