title: Micro RT-PCR.
abstract: This unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 microg).
authors.affiliation: National Cancer Institute/NIH, Bethesda, Maryland, USA.
authors.forename: C P
authors.initials: CP
authors.lastname: Paweletz
authors.forename: L
authors.initials: L
authors.lastname: Charboneau
authors.forename: L A
authors.initials: LA
authors.lastname: Liotta
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D006801:Humans; D042282:Microdissection; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification* / Q000592:standards; D012194:RNA-Directed DNA Polymerase; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods
chemical_list: D012313:RNA; D012194:RNA-Directed DNA Polymerase