The 20-minute rapid MART-1 immunostain for malignant melanoma frozen sections.
BACKGROUND
Immunohistochemical staining has been used to help detect malignant melanoma on Mohs surgery frozen sections. Previous investigators have developed protocols for reliable MART-1 immunostaining of frozen sections, but these protocols are time-consuming.


OBJECTIVE
The objective was to report a rapid 20-minute MART-1 immunostaining protocol for frozen sections.


METHODS
The protocol was utilized on 30 melanomas treated with Mohs micrographic surgery.


RESULTS
The stain clearly highlighted normal background melanocytes, as well as melanocytic hyperplasia and malignant melanoma.


CONCLUSIONS
The 20-minute protocol provides a rapid and reliable method for immunostaining of malignant melanoma. The availability of more rapid immunostaining methods improves efficiency of the Mohs laboratory and significantly reduces patient and physician waiting time. The authors have indicated no significant interest with commercial supporters.
Kimyai-Asadi
Arash
A
DermSurgery Associates, Houston, Texas 77030, USA. akimyai@yahoo.com
Ayala
Gabriel B
GB
Goldberg
Leonard H
LH
Vujevich
Justin
J
Jih
Ming H
MH
D000951:Antigens, Neoplasm; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D058965:MART-1 Antigen; D008545:Melanoma / Q000473:pathology* / Q000601:surgery; D015580:Mohs Surgery; D009363:Neoplasm Proteins; D015203:Reproducibility of Results; D012878:Skin Neoplasms / Q000473:pathology* / Q000601:surgery; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D046509:Tissue Culture Techniques
D000951:Antigens, Neoplasm; D058965:MART-1 Antigen; C548667:MLANA protein, human; D009363:Neoplasm Proteins