Detection of carbohydrate-binding proteins by oligosaccharide-modified polypyrrole interfaces using electrochemical surface plasmon resonance.
This paper reports on the use of electrochemical surface plasmon resonance (E-SPR) for the detection of carbohydrate-binding proteins. The generation of an SPR sensor specific to lectins Arachis hypogaea (PNA) and Maackia amurensis (MAA) is based on the electrochemical polymerization of oligosaccharide derivatives functionalized by pyrrole groups. The resulting thin conducting polymer films were characterized using E-SPR and atomic force microscopy (AFM). The specific binding of PNA to polypyrrole-lactosyl and of MAA to polypyrrole-3'-sialyllactosyl films was investigated using SPR. The detection limit was 41 nM for PNA and 83 nM for MAA. Through Scatchard analysis and linear transformation of the SPR sensorgram data, association (k(ass)) and dissociation rate constants (k(diss)) could be determined.
Gondran
Chantal
C
Département de Chimie moléculaire (UMR-CNRS-5250), Institut de Chimie Moléculaire de Grenoble (FR-CNRS 2607), Université Joseph Fourier, BP 53, Grenoble Cedex 9, France.
Dubois
Marie-Pierre
MP
Fort
Sébastien
S
Cosnier
Serge
S
Szunerits
Sabine
S
D010367:Arachis; D037102:Lectins / Q000032:analysis*; D029901:Maackia; D009844:Oligosaccharides; D011108:Polymers; D011758:Pyrroles; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*
D037102:Lectins; D009844:Oligosaccharides; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole