title: Overexpression of ADH1 and HXT1 genes in the yeast Saccharomyces cerevisiae improves the fermentative efficiency during tequila elaboration.
abstract: This work assessed the effect of the overexpression of ADH1 and HXT1 genes in the Saccharomyces cerevisiae AR5 strain during fermentation of Agave tequilana Weber blue variety must. Both genes were cloned individually and simultaneously into a yeast centromere plasmid. Two transformant strains overexpressing ADH1 and HXT1 individually and one strain overexpressing both genes were randomly selected and named A1, A3 and A5 respectively. Overexpression effect on growth and ethanol production of the A1, A3 and A5 strains was evaluated in fermentative conditions in A. tequilana Weber blue variety must and YPD medium. During growth in YPD and Agave media, all the recombinant strains showed lower cell mass formation than the wild type AR5 strain. Adh enzymatic activity in the recombinant strains A1 and A5 cultivated in A. tequilana and YPD medium was higher than in the wild type. The overexpression of both genes individually and simultaneously had no significant effect on ethanol formation; however, the fermentative efficiency of the A5 strain increased from 80.33% to 84.57% and 89.40% to 94.29% in YPD and Agave medium respectively.
authors.affiliation: Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors.forename: Melesio
authors.initials: M
authors.lastname: Gutiérrez-Lomelí
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Torres-Guzmán
authors.forename: Gloria Angélica
authors.initials: GA
authors.lastname: González-Hernández
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Cira-Chávez
authors.forename: Carlos
authors.initials: C
authors.lastname: Pelayo-Ortiz
authors.forename: Jose de Jesús
authors.initials: Jde J
authors.lastname: Ramírez-Córdova
mesh_terms: D027882:Agave / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000434:Alcoholic Beverages / Q000382:microbiology*; D018533:Biomass; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D051246:Glucose Transport Proteins, Facilitative; D005990:Glycerol / Q000378:metabolism; D007218:Industrial Microbiology; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D051246:Glucose Transport Proteins, Facilitative; C090594:HXT1 protein, S cerevisiae; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D000431:Ethanol; C516801:ADH1 protein, S cerevisiae; D000426:Alcohol Dehydrogenase; D005990:Glycerol