[Comparison of the antigenic spectrum A1, A2, and Ax erythrocytes: inhibition of mab with glucoconjugates of lipid and protein nature with different isoelectric properties].
The inhibition of monoclonal antibodies (MAbs) (anti-A1 2-24, anti-A 2-22, anti- 2-19, and anti- A 2-18; Workshop IV) by glycotopes (glycoconjugates) obtained by the enzymatic treatment of A1, A2, and Ax erythrocytes and by chloroform-methanol method from the membranes of these erythrocytes, followed by ion-exchange gel chromatography was evaluated. According to specificity and isoelectric properties, glycoconjugates (in the sequence of desorption from an anion exchanger) of glycoprotein (A(pr-00), A(pr-0), A(pr-1), A(pr-3)) and glycosphingolipid (A1p,00, A(1p-0), A(1p-1), A(1p-3), A(1p-3)) origin were identified. A1 erythrocytes showed the broad-spectrum inhibiting activity of glycoconjugates, which being at maximum with acid A(1p-00) and A(pr-3) (the ionic points of pH 6.55 and 6.45), as well as with A(lp-00) and A(1p-00) 1 The inhibition of A(pr-3) from A2 was significantly weaker that that from A1. An A(pr-3) fraction from Ax erythrocytes inhibited as strongly as in A1 and A2. The inhibition of A(pr-3p) from Ax either failed to develop at all (MAb 2-19) or turned out be weakened (MAbs 2-22 and 2-18). Thus, the cascade decrease in agglutinogenicity in the order of A1, A2, and Ax correlated with the reduction in the inhibiting capacity of A(pr-3) in all the study groups, including Ax.
Delevskiĭ
Iu P
IuP
Zinchenko
A A
AA
D000017:ABO Blood-Group System / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002849:Chromatography, Gas; D000939:Epitopes / Q000276:immunology*; D004912:Erythrocytes / Q000737:chemistry / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007526:Isoelectric Point
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids