title: Investigation of NAD(P)H:quinone oxidoreductase 1 (NQO1) C609T polymorphism in prostate cancer.
abstract: BACKGROUND
  NAD(P)H:quinone oxidoreductase 1 (NQO1) is a part of the antioxidant defense system. NQO1 protects cells from oxidative stress by maintaining antioxidant forms of ubiquinone and vitamin E and this enzymatic activity can be sufficient to protect against carcinogenesis. Oxidative stress may contribute to carcinogenesis as an important risk factor. This study aimed to investigate the relationship between prostate cancer and NQO1 C609T polymorphism in a Turkish population.
  
  
  METHODS
  Forty-five patients with prostate cancer and fifty healthy controls were included in this study. Gene polymorphism was determined by a restricted fragment length polymorphism-polymerase chain reaction (PCR-RFLP) method.
  
  
  RESULTS
  The NQO1 TT genotype was demonstrated to be increased in patients, there were no significant differences between distributions of NQO1 C609T genotypes in the study groups. Serum PSA and alkaline phosphatase levels were elevated in patients carrying T alleles (TT > CT > CC). There were no correlations between tumor size, node classification, metastasis or stage and NQO1 genotypes.
  
  
  CONCLUSIONS
  The results of our study of Turkish prostate cancer patients suggests that mutation of the NQO1 gene may effect the serum PSA and alkaline phosphatase levels. However there were no differences between the NQO1 genotypes in the study groups.
authors.affiliation: Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul University, Capa-Istanbul, Turkey.
authors.forename: H Arzu
authors.initials: HA
authors.lastname: Ergen
authors.forename: Uzay
authors.initials: U
authors.lastname: Gormus
authors.forename: Fehmi
authors.initials: F
authors.lastname: Narter
authors.forename: Umit
authors.initials: U
authors.lastname: Zeybek
authors.forename: Sibel
authors.initials: S
authors.lastname: Bulgurcuoglu
authors.forename: Turgay
authors.initials: T
authors.lastname: Isbir
mesh_terms: D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D000483:Alleles; D015992:Body Mass Index; D003404:Creatinine / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D014421:Turkey
chemical_list: D003404:Creatinine; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D000469:Alkaline Phosphatase; D017430:Prostate-Specific Antigen