Expression of cellular isoform of prion protein on the surface of peripheral blood lymphocytes among women exposed to low doses of ionizing radiation.
Ionizing radiation affects the expression of adhesive and co-stimulatory molecules in lymphocytes. The physiological function of cellular isoform of prion protein (PrPc) is little known. Evidences indicate a link between lymphocytes activation and PrPc expression on their surface; however, no direct effect of radiation on PrPc level in these cells was investigated. The objective of this study was to determinate the effect of low doses of ionizing radiation on the expression of PrPc on the surface peripheral blood lymphocytes in the women operating X-ray equipment. In 36 female workers and 30 persons of the control group the PrPc expression on CD3 (T lymphocytes), CD4 (T helper), CD8 (T cytotoxic) and CD19 (B lymphocytes), as well as the percentage of lymphocytes with PrPc on their surface, were tested. Subgroups with respect to age and length of employment were selected. A significant increase was observed in PrPc expression on CD3 and CD4 with lowered PrPc level on CD8 and percentage of CD8 cells with PrPc in workers compared to control. The PrPc level did not show significant changes in subgroups in relation to age (below and over 40 years old) both in the investigated and control groups, whereas a lower percentage of PrPc expressing CD19 cells showed in employed women below 40 years of age. A significant decrease was found in PrPc expression on the surface of CD3, CD4 and CD8 cells in the subgroup employed for over 10 years than in the subgroup with less than 10 years of employment.
Kłuciński
Piotr
P
Department of Medical Microbiology, Medical University of Silesia, Katowice, Poland.
Mazur
Bogdan
B
Hrycek
Antoni
A
Maśluch
Ewa
E
Cieślik
Paweł
P
Kaufman
Jolanta
J
Martirosian
Gayane
G
D000328:Adult; D000367:Age Factors; D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000276:immunology* / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000528:radiation effects; D008875:Middle Aged; D011328:Prions / Q000032:analysis*; D011839:Radiation, Ionizing; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology
D018941:Antigens, CD19; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D011328:Prions