title: Differential control of isocitrate lyase gene transcription by non-fermentable carbon sources in the milk yeast Kluyveromyces lactis.
abstract: The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription factor and KlSip4p binds to the promoter elements. Glycerol diminishes KlICL1 expression and a single carbon source responsive element (CSRE) sequence is both necessary and sufficient to mediate this regulation. The glycerol effect is less pronounced in Saccharomyces cerevisiae than in K. lactis. Mutants lacking KlGUT2 (which encodes the glycerol 3-phosphate dehydrogenase) still show reduced expression in glycerol, whereas mutants deficient in glycerol kinase (Klgut1) do not. We conclude that a metabolite of glycerol is required for this regulation.
authors.affiliation: Departamento de Bioquímica y Biología Molecular and Instituto Universitario de Biotecnología de Asturias, Facultad de Medicina, Universidad de Oviedo, Campus del Cristo, Oviedo, Spain. Rosaura@fq.uniovi.es
authors.forename: Rosaura
authors.initials: R
authors.lastname: Rodicio
authors.forename: Maria Luz
authors.initials: ML
authors.lastname: López
authors.forename: Sara
authors.initials: S
authors.lastname: Cuadrado
authors.forename: Alejandra F
authors.initials: AF
authors.lastname: Cid
authors.forename: Begoña
authors.initials: B
authors.lastname: Redruello
authors.forename: Fernando
authors.initials: F
authors.lastname: Moreno
authors.forename: Jürgen J
authors.initials: JJ
authors.lastname: Heinisch
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Hegewald
authors.forename: Karin D
authors.initials: KD
authors.lastname: Breunig
mesh_terms: D000818:Animals; D001483:Base Sequence; D002244:Carbon / Q000494:pharmacology*; D047369:Chromatin Immunoprecipitation; D005285:Fermentation / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D005991:Glycerol Kinase / Q000378:metabolism; D007522:Isocitrate Lyase / Q000096:biosynthesis / Q000235:genetics*; D007716:Kluyveromyces / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D012333:RNA, Messenger; D002244:Carbon; D005991:Glycerol Kinase; D007522:Isocitrate Lyase; D005990:Glycerol