[Tissue culture and regeneration of Sedum hybridum].
The cotyledons and hypecotyl of Sedum hybridum were used as explant to induce calluse on MS media supplemented with different concentration of hormone. There were formed two kind of calluses, one was red while other was green. The cotyledon was the ideal explant for the callus induction, its induction rate could be reached 80% - 82% either on MS medium with 6-BA 1 mg/L and 2,4-D 0.5 mg/L or with 6-BA 1 mg/L and NAA 0.5 mg/L. Numerous adventitious buds could formed from calluses on the MS medium with 6-BA 2 mg/L and NAA 0.5 mg/L. The medium for the root growth was 1/2 MS. The tube seedling which can be successfully transplanted at 80% survival.
Zhao
Yin-ping
YP
Department of Life Science, Xi' an College of Arts and Sciences, Xi' an 710065, China. yinping019@163.com
Hu
Jie
J
Zhang
Jiu-dong
JD
D029627:Crassulaceae / Q000187:drug effects / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D010946:Plants, Medicinal / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000379:methods
D003470:Culture Media; D010937:Plant Growth Regulators