Systems for applied gene control in Saccharomyces cerevisiae.
Saccharomyces cerevisiae is frequently used in biotechnology, including fermentative processes in food production, heterologous protein production and high throughput developments for biomedicine. Accurate expression of selected genes is essential for all these areas. Systems that can be regulated are particularly useful because they allow controlling the timing and levels of gene expression. We examine here new expression systems that have been described, including improvements of classical ones and new strategies of artificial gene control that have been applied in functional genomics.
Maya
Douglas
D
Departamento de Genética, Universidad de Sevilla, Avda. Reina Mercedes 6, 41012, Seville, Spain.
Quintero
María José
MJ
de la Cruz Muñoz-Centeno
María
M
Chávez
Sebastián
S
D000693:Anaerobiosis; D002352:Carrier Proteins / Q000235:genetics; D005686:Galactokinase / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005818:Genetic Engineering; D023281:Genomics; D008668:Metallothionein; D009875:Operator Regions, Genetic; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014158:Transcription, Genetic; D019076:Transgenes
C500769:CUP1-1 protein, S cerevisiae; D002352:Carrier Proteins; D029701:Saccharomyces cerevisiae Proteins; D008668:Metallothionein; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase