[Determination of chloropromazine residues in animals kidney and urine using LC-MS/MS method].
Chloropromazina (CP) is subjected to monitoring food animals products, with a minimum required performance limit (MPRL) set 5.0 microg/kg. Homogenized kidney and urine were extracted with acetonitrile. CP- d3 was used as internal standard. LC separation was done on Luna C18 150 x 2 mm, 5 microm column in mobile phase acetonitrile-acetic acid. CP was determination by LC-ESI-MS/MS negative mode. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 5.0 ng/g were in the range 84-102%. The limit of decision (CCalpha) and detection capability (CCbeta) CP in kidney were 1.19; 2.87 ng/g and urine 1.08; 2.61 ng/g.
Rodziewicz
Lech
L
Pracownia Badań Chemicznych Srodków Spozywczych, Zakład Higieny Weterynaryjnej 15-959 Białystok, ul. Zwyciestwa 26a. rodziewicz@wiw.bianet.com.pl
Zawadzka
Iwona
I
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000652:urine*; D002701:Chloramphenicol / Q000032:analysis* / Q000652:urine*; D004350:Drug Residues / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D007668:Kidney / Q000737:chemistry*; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol