Expression and regulation of major histocompatibility complex on neural stem cells and their lineages.
The expression of major histocompatibility complex (MHC) antigens on neural stem cells (NSCs) and their lineages is tightly related to the fate of these cells as grafts in allogenic transplantation. In this study, we observed that NSCs derived from embryonic rat forebrain expressed MHC class I and class II molecules at a low level, whereas the cells differentiated from NSCs, including neurons, astrocytes, and oligodendrocytes, lost their MHC expression. However, a proinflammatory factor, interferon-gamma (IFN-gamma), could induce and up-regulate the expression of MHC in both NSCs and their differentiated lineages in vitro. These results suggest that predifferentiating NSCs into lineage-limited cells prior to transplantation combined with controlling the local production of proinflammatory cytokines moderately may potentially benefit the survival of transplants.
Yin
Lan
L
Department of Neurobiology, Shanghai Jiaotong University School of Medicine, Shanghai, PR China.
Fu
Sai-Li
SL
Shi
Gui-Ying
GY
Li
Ying
Y
Jin
Jian-Qiang
JQ
Ma
Zheng-Wen
ZW
Lu
Pei-Hua
PH
D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D005786:Gene Expression Regulation / Q000187:drug effects; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology; D008285:Major Histocompatibility Complex / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000276:immunology; D016548:Prosencephalon / Q000166:cytology / Q000196:embryology; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma