title: Purification of vitronectin.
abstract: This unit describes the purification of extracellular vitronectin from plasma by a series of affinity chromatography steps. First plasma is depleted of fibronectin and other heparin-binding proteins; the sample is then treated with urea to activate the heparin-binding activity of vitronectin. The resulting vitronectin is approximately 98% pure.
authors.affiliation: Chapel Hill, North Carolina, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Akiyama
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D019096:Vitronectin / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D019096:Vitronectin