Association between the -2518G/A polymorphism in the monocyte chemoattractant protein-1 (MCP-1) gene and myocardial infarction in Tunisian patients.
BACKGROUND
Monocyte chemoattractant protein-1 (MCP-1; gene name CCL2) has been suggested to play an important role in the initiation of atherosclerosis by recruiting monocytes to sites of injured endothelium. Recently, single nucleotide polymorphisms (SNPs) in the MCP-1 regulatory region have been identified. Controversial results regarding the association of the -2518G/A polymorphism of the MCP-1 gene with coronary artery disease (CAD) have been reported. In the present study, we examined a possible association between the -2518G/A polymorphism of the MCP-1 gene and myocardial infarction (MI) in a sample of the Tunisian population.


METHODS
A total of 319 Tunisian patients with MI and 467 healthy controls were included in the study. The SNP of the MCP-1 gene was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.


RESULTS
Patients with MI had significantly higher frequency of the AG+GG genotypes compared to controls [42.9% vs. 35.8%; OR (95%CI), 1.34 (1.00-1.79); p=0.04]. The MI patient group showed a significant higher frequency of the G allele compared to the controls [0.242 vs. 0.195; OR (95%CI), 1.31(1.02-1.68), p=0.03]. The association between the -2518G/A polymorphism of the MCP-1 gene and MI was no longer significant after adjustment for other well-established risk factors.


CONCLUSIONS
The present study showed a significant but not independent association between the -2518G/A polymorphism of the MCP-1 gene (presence of G allele) and MI in the Tunisian population.
Jemaa
Riadh
R
Research Laboratory LAB-SM-01, Department of Biochemistry, Hospital la Rabta, Tunis, Tunisia. jemaa_riadh@yahoo.fr
Rojbani
Hajer
H
Kallel
Amani
A
Ben Ali
Samir
S
Feki
Moncef
M
Chabrak
Sonia
S
Elasmi
Monia
M
Taieb
Samah Haj
SH
Sanhaji
Haïfa
H
Souheil
Omar
O
Mechmeche
Rachid
R
Kaabachi
Naziha
N
D001483:Base Sequence; D018932:Chemokine CCL2 / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014416:Tunisia
D018932:Chemokine CCL2; D017931:DNA Primers