title: Immunofluorescence staining.
abstract: This unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. This provides both a visible signal and amplification of the signal and the results are observed with a fluorescent microscope.
authors.affiliation: National Heart, Lung, and Blood Institute/NIH, Bethesda, Maryland, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Donaldson
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D002478:Cells, Cultured / Q000737:chemistry / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence / Q000379:methods*; D013048:Specimen Handling / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents