Molecular homogeneity in diverse geographical populations of Phlebotomus papatasi (Diptera, Psychodidae) inferred from ND4 mtDNA and ITS2 rDNA Epidemiological consequences.
An intraspecific study on Phlebotomus papatasi, the main proven vector of Leishmania major among the members of the subgenus Phlebotomus, was performed. The internal transcribed spacer 2 (ITS 2) of rDNA and the ND4 gene of mt DNA were sequenced from 26 populations from 18 countries (Albania, Algeria, Cyprus, Egypt, Greece, India, Iran, Israel, Italy, Lebanon, Morocco, Saudi Arabia, Spain, Syria, Tunisia, Turkey, Yugoslavia and Yemen), and compared. Samples also included three other species belonging to the subgenus Phlebotomus: P. duboscqi, a proven vector of L. major in the south of Sahara (three populations from Burkina Faso, Kenya and Senegal), P. bergeroti, a suspected vector of L. major (three populations from Oman Sultanate, Iran and Egypt), and one population of P. salehi from Iran. A phylogenetic study was carried out on the subgenus Phlebotomus. Our results confirm the validity of the morphologically characterized taxa. The position of P. salehi is doubtful. Variability in P. papatasi contrasts with that observed within other species having a wide distribution like P. (Paraphlebotomus) sergenti in the Old World or Lutzomyia (Lutzomyia) longipalpis in the New World. Consequently, it could be hypothesized that all populations of P. papatasi over its distribution area have similar vectorial capacities. The limits of the distribution area of L. major are correlated with the distribution of common rodents acting as hosts of the parasites.
Depaquit
Jérôme
J
USC "VECPAR", AFSSA, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France. jerome.depaquit@univ-reims.fr
Lienard
Emmanuel
E
Verzeaux-Griffon
Astrid
A
Ferté
Hubert
H
Bounamous
Azzedine
A
Gantier
Jean-Charles
JC
Hanafi
Hanafi A
HA
Jacobson
Raymond L
RL
Maroli
Michele
M
Moin-Vaziri
Vahideh
V
Müller
Frédérique
F
Ozbel
Yusuf
Y
Svobodova
Milena
M
Volf
Petr
P
Léger
Nicole
N
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D006801:Humans; D009245:NADH Dehydrogenase / Q000235:genetics*; D010691:Phlebotomus / Q000235:genetics*; D010217:Phlebotomus Fever / Q000453:epidemiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; C098483:NADH dehydrogenase subunit 4; D009245:NADH Dehydrogenase