Complexes of DOTA-bisphosphonate conjugates: probes for determination of adsorption capacity and affinity constants of hydroxyapatite.
The adsorption on hydroxyapatite of three conjugates of a bisphosphonate and a macrocycle having C1, C2, and C3 spacers and their terbium complexes was studied by the radiotracer method using 160Tb as the label. The radiotracer-containing complex of the conjugate with the C3 spacer was used as a probe for the determination of the adsorption parameters of other bisphosphonates that lack a DOTA unit. A physicochemical model describing the competitive adsorption was successfully applied in the fitting of the obtained data. The maximum adsorption capacity of bisphosphonates containing bulky substituents is determined mainly by their size. For bisphosphonates having no DOTA moiety, the maximum adsorption capacity is determined by the electrostatic repulsion between negatively charged bisphosphonate groups. Compounds with a hydroxy or amino group attached to the alpha-carbon atom show higher affinities. Macrocyclic compounds containing a short spacer between the different bisphosphonic acid groups and the macrocyclic unit exhibit high affinities, indicating a synergic effect of the bisphosphonic and the macrocyclic groups during adsorption. The competition method described uses a well-characterized complex and allows a simple evaluation of the adsorption behavior of bisphosphonates. The application of the macrocycle-bisphosphonate conjugates allows easy radiolabeling via complexation of a suitable metal isotope.
Vitha
Tomas
T
Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL Delft, The Netherlands.
Kubícek
Vojtech
V
Hermann
Petr
P
Kolar
Zvonimir I
ZI
Wolterbeek
Hubert Th
HT
Peters
Joop A
JA
Lukes
Ivan
I
D000327:Adsorption; D001667:Binding, Competitive; D004164:Diphosphonates / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D015394:Molecular Structure
D004164:Diphosphonates; D006573:Heterocyclic Compounds, 1-Ring; D046911:Macromolecular Substances; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D017886:Durapatite