Translocation of VP1686 upregulates RhoB and accelerates phagocytic activity of macrophage through actin remodeling.
Here, we report that Vibrio parahaemolyticus induces a rapid remodeling of macrophage actin and activates RhoB GTPase. Mutational analysis revealed that the effects depend on type III secretion system 1 regulated translocation of a V. parahaemolyticus effector protein, VP1686, into the macrophages. Remodeling of actin is shown to be necessary for increased bacterial uptake followed by initiation of apoptosis in macrophages. This provides evidence for functional association of the VP1686 in triggering an eat-me-and-die signal to the host.
Bhattacharjee
Rabindra N
RN
Akira Innate Immunity Project, Exploratory Research for Advance Technology, Japan Science and Technology Agency, Osaka University, Osaka 565-0871, Japan.
Park
Kwon-sam
KS
Chen
Xiuhao
X
Iida
Tetsuya
T
Honda
Takeshi
T
Takeuchi
Osamu
O
Akira
Shizuo
S
D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D010587:Phagocytosis; D015854:Up-Regulation; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D020743:rhoB GTP-Binding Protein / Q000378:metabolism*
D000199:Actins; D001426:Bacterial Proteins; D020743:rhoB GTP-Binding Protein