Agarose gel electrophoresis of proteins.
This unit describes electrophoretic separation and identification of large proteins from a complex protein mixture. Agarose gel is utilized for the electrophoretic matrix, and detection of proteins is accomplished by transfer of the proteins to a membrane that is probed with specific antibodies and chemiluminescence reagents. Alternatively the protein can be detected in the gel using radiolabeled antibodies and autoradiography.
Krizek
Dennis M
DM
National Institutes of Health, Bethesda, Maryland, USA.
Rick
Margaret E
ME
D001345:Autoradiography / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D049449:Luminescence; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D040901:Proteomics / Q000379:methods*; D014841:von Willebrand Factor / Q000032:analysis / Q000276:immunology
D011506:Proteins; D014841:von Willebrand Factor