Profiling of differentially expressed genes in porcine epithelial cells derived from periodontal ligament and gingiva by DNA microarray.
OBJECTIVE
It is unknown which genes are differentially expressed in cultured epithelial cells derived from the epithelial rests of Malassez (ERM) in periodontal ligament and oral gingival epithelium (OGE). This study analysed the different gene expression of OGE and ERM cells using a DNA microarray technique.


METHODS
Epithelial cells from ERM and OGE were isolated from porcine periodontal ligament and oral gingival epithelium. Each RNA sample extracted from the cells was reverse transcribed into cDNA and labelled with either cytidine 5-dUTP (Cy5) or cytidine 3-dUTP (Cy3). These labelled cDNA probes were then mixed and simultaneously hybridised to the Pig 13K microarray plate bearing 13,295 different genes (Operon, AL). Cellular enzyme-linked immunosorbent assay (CELISA) was performed to confirm the expression at protein level.


RESULTS
There were nine genes common to the triplicate microarrays in ERM cells and one in OGE cells. Four of the nine genes including tissue factor (TF), FAT cadherin (FAT) and two unknown genes were expressed at levels more than threefold higher in ERM cells than in OGE cells. The protein levels of both TF and FAT in ERM cells were significantly higher than those in OGE.


CONCLUSIONS
TF and FAT may act as markers to distinguish ERM cells from OGE cells in vitro.
Kurashige
Yoshihito
Y
Division of Pediatric Dentistry, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan.
Saitoh
Masato
M
Nishimura
Michiko
M
Noro
Daisuke
D
Kaku
Tohru
T
Igarashi
Seiji
S
Takuma
Taishin
T
Arakawa
Toshiya
T
Inoue
Takashi
T
Abiko
Yoshihiro
Y
D000818:Animals; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D046228:Microarray Analysis; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*; D013925:Thromboplastin / Q000235:genetics* / Q000378:metabolism
D015820:Cadherins; D013925:Thromboplastin