title: A controllable solid-state Ru(bpy)(3)(2+) electrochemiluminescence film based on conformation change of ferrocene-labeled DNA molecular beacon.
abstract: A controllable solid-state electrochemiluminescence (ECL) film based on efficient and stable quenching of ECL of ruthenium(II) tris-(bipyridine) (Ru(bpy)32+) by oxidizing ferrocene (Fc) at the electrode is developed. The ECL intensity is correlated to the distance which is controlled by the conformation of the ferrocene-labeled DNA molecular beacon (Fc-MB) between the Fc and Ru(bpy)32+ immobilized on the electrode. The conformation adjustment is conducted via complementary DNA hybridizing with the bases in the loop of the Fc-MB and changing the temperature of the Fc-MB and the resultant double-stranded DNA (dsDNA). Those events all result in change of the ECL intensity. With such characteristics, the solid-state Ru(bpy)32+-ECL film has the potential to be applied to reagentless DNA ECL biosensors and to calculate thermodynamic parameters of equilibrium constants of MB binding and the stem-loop formation.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, People's Republic of China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Yun
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Jingming
authors.initials: J
authors.lastname: Zhou
authors.forename: Pingang
authors.initials: P
authors.lastname: He
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Fang
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006358:Hot Temperature; D049449:Luminescence; D000075163:Metallocenes; D008956:Models, Chemical; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009942:Organometallic Compounds / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D009942:Organometallic Compounds; C469719:ruthenium (bpy)3(2+)-C2O4(2-); D015082:2,2'-Dipyridyl; D004247:DNA; C004998:ferrocene