Preparation and culture of human lymphocytes.
This unit presents protocols for preparation of lymphocytes from peripheral (whole) blood or leucopherisis samples, first by differential centrifugation in Ficoll-Hypaque to separate them from erythrocytes and granulocytes. Repeated centrifugation removes the plasma and platelets. Monocyte/macrophage cells and dendritic cells can be purified from the T and B cell population by exploiting their adhesion to serum-coated plates in the presence of recombinant IL-3. Different subpopulations of lymphocytes can be isolated by specific antibodies bound to magnetic beads.
Biddison
W E
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National Institute of Neurological Disorders and Stroke/NIH, Bethesda, Maryland, USA.
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology