title: Characterization and expression of an nsLTPs-like antimicrobial protein gene from motherwort (Leonurus japonicus).
abstract: In screening for potent antimicrobial proteins (AMPs) from plant seeds, we had purified a heat-stable AMP, LJAMP2, from the seeds of a medicine herb, motherwort (Leonurus japonicus Houtt). In an in vitro assay, the protein can inhibit the growth of both fungi and bacteria. Then a cDNA encoding LJAMP2 was cloned by the rapid amplification of cDNA ends based on the N-terminal amino acid sequence determined. The deduced amino acid sequences of this cDNA show similarity to plant non-specific lipid transfer proteins. Northern blotting assay revealed that this nsLTP-like gene, designated LJAMP2, was expressed in seeds. Overexpression of LJAMP2 in tobacco enhanced resistance to the fungal pathogen Alternaria alternata and the bacterial pathogen Ralstonia solanacearum, significantly, while no visible alteration in plant growth and development. Our data confirm the antifungal and antibacterial function of LJAMP2 from motherwort seeds and suggest the potential of LJAMP2 in improving disease resistance in plants.
authors.affiliation: Biotechnology Research Center, Southwest University, Chongqing, 400716, China. yangxy94@swu.edu.cn
authors.forename: Xingyong
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Wang
authors.forename: Xianbi
authors.initials: X
authors.lastname: Li
authors.forename: Beibei
authors.initials: B
authors.lastname: Zhang
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Xiao
authors.forename: Demou
authors.initials: D
authors.lastname: Li
authors.forename: Chengjian
authors.initials: C
authors.lastname: Xie
authors.forename: Yan
authors.initials: Y
authors.lastname: Pei
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D031332:Leonurus / Q000378:metabolism*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology / Q000502:physiology*
chemical_list: D010940:Plant Proteins