Effects of cooling and warming on 5-hydroxytryptamine- and acetylcholine-induced contractions of human umbilical vessels: role of nitric oxide.
The effects of cooling (to 28 degrees C) and warming (to 41 degrees C) on the vasoconstrictions induced by 5-hydroxytryptamine (5-HT) and acetylcholine (ACh) and the role of nitric oxide in these effects were analyzed in human umbilical artery and vein. 5-HT (10(-9)-10(-4) M) and ACh (10(-9)-10(-4) M) induced concentration-dependent contractions at 37, 28 and 41 degrees C. During cooling, the sensitivity, but not the maximal response, of 5-HT and ACh was significantly higher than at 37 degrees C; and during warming, again the sensitivity, but not the maximal response, of both contractile agents was significantly lower than at 37 degrees C. Neither cooling to 28 degrees C nor warming to 41 degrees C, after treatment with N(G)-nitro-L-arginine methyl esther (L-NAME, 10(-4) M), modify the effect of temperature in both vessels. These results suggest that cooling- and warming-induced responses in human umbilical artery and vein are independent of nitric oxide.
Atalik
K E
KE
Department of Pharmacology, Faculty of Meram Medicine, University of Selçuk, Konya 42080, Turkey. esraatalik@hotmail.com
Kiliç
M
M
Nurullahoğlu
Z U
ZU
Doğan
N
N
D000109:Acetylcholine; D003080:Cold Temperature; D003404:Creatinine; D004338:Drug Combinations; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors; D012701:Serotonin; D014469:Umbilical Arteries / Q000187:drug effects / Q000502:physiology*; D014471:Umbilical Veins / Q000187:drug effects / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects
D004338:Drug Combinations; C014050:creatinine sulfate, serotonin drug combination; D009569:Nitric Oxide; D012701:Serotonin; D003404:Creatinine; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester