Negative regulation of caspase-3 expression in the neonatal cerebral cortex by alpha2A-adrenoceptors.
Antisense oligonucleotide to alpha2A-adrenoceptors increased the levels of mRNA (reverse polymerase chain reaction) and protein for a key executioner protease of apoptosis caspase-3 (immunoblotting assay) in the cerebral cortex of newborn rats. The relationship between the observed effect and low expression of alpha2-adrenoceptors was confirmed by the possibility of correcting this phenomenon by clonidine (stimulator of alpha2-adrenoceptors).
Men'shanov
P N
PN
Laboratory of Functional Neurogenomics, Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk.
Bannova
A V
AV
Il'inykh
F A
FA
Dygalo
N N
NN
D058669:Adrenergic alpha-2 Receptor Antagonists; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D001483:Base Sequence; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D061945:Caspase Inhibitors; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018341:Receptors, Adrenergic, alpha-2 / Q000235:genetics / Q000378:metabolism
C547770:Adra2a protein, rat; D058669:Adrenergic alpha-2 Receptor Antagonists; D061945:Caspase Inhibitors; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D018341:Receptors, Adrenergic, alpha-2; C505410:Casp3 protein, rat; D053148:Caspase 3