[Dose-effect relationship between premature chromosome and irradiation dose].
OBJECTIVE
To explore the dose-effect relationship between premature chromosome condensation induced by Calyculin A and irradiation dose.


METHODS
The human peripheral blood was irradiated by (137)Cs gamma radial. The irradiation dose included 0, 0.5, 1.0, 2.0, 3.0, 4.0, 5.0 Gy. The premature chromosome condensation induced by Calyculin A was observed, and dyed by centromeric banding.


RESULTS
There was the quadratic relation between the total aberration, fragment, dicentric+centric ring (dic+r) ration and irradiation dose.


CONCLUSIONS
Premature chromosome condensation induced by Calyculin A can be used as a biodosimetry.
Jiang
Bo
B
Institute of Radiation Medicine, Chinese Academy of Medical Science, Tianjin 300020, China.
Liu
Qiang
Q
Jiang
En-Hai
EH
Xing
Zhi-Wei
ZW
Zhao
Xin-Ran
XR
D002460:Cell Line; D002869:Chromosome Aberrations / Q000528:radiation effects*; D002871:Chromosome Banding; D002875:Chromosomes / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D008387:Marine Toxins; D010080:Oxazoles / Q000494:pharmacology; D055815:Young Adult
D008387:Marine Toxins; D010080:Oxazoles; C059041:calyculin A