title: Assessing the bioavailability of phenanthrene to soil microorganisms using the Tenax extraction method.
abstract: The Tenax TA extraction technique followed by gas chromatography mass spectrometry (GC-MS) determinations was used to assess the actually bioavailable fraction of phenanthrene (ABF-Phe) in three different soils freshly contaminated with this compound at levels of 10, 100, and 1,000 mg kg(-1). The results were related to the ecotoxic effect of phenanthrene on soil nitrifying bacteria. Nitrification potential measurements were applied as an ecotoxicity end point. A strong positive linear relationship (r2 = 0.95) was obtained between the content of the actually bioavailable phenanthrene fraction and the inhibition of nitrifying bacteria activity.
authors.affiliation: Institute of Soil Science and Plant Cultivation, National Research Institute, ul. Czartoryskich 8, 24-100, Pulawy, Poland. bozenas@iung.pulawy.pl
authors.forename: Bozena
authors.initials: B
authors.lastname: Smreczak
authors.forename: Barbara
authors.initials: B
authors.lastname: Maliszewska-Kordybach
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Klimkowicz-Pawlas
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010616:Phenanthrenes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D011108:Polymers / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity
chemical_list: D009573:Nitrites; D010616:Phenanthrenes; D011108:Polymers; D012989:Soil Pollutants; C010514:tenax; C031181:phenanthrene