title: Mutations in NALP12 cause hereditary periodic fever syndromes.
abstract: NALP proteins, also known as NLRPs, belong to the CATERPILLER protein family involved, like Toll-like receptors, in the recognition of microbial molecules and the subsequent activation of inflammatory and immune responses. Current advances in the function of NALPs support the recently proposed model of a disease continuum bridging autoimmune and autoinflammatory disorders. Among these diseases, hereditary periodic fevers (HPFs) are Mendelian disorders associated with sequence variations in very few genes; these variations are mostly missense mutations whose deleterious effect, which is particularly difficult to assess, is often questionable. The growing number of identified sporadic cases of periodic fever syndrome, together with the lack of discriminatory clinical criteria, has greatly hampered the identification of new disease-causing genes, a step that is, however, essential for appropriate management of these disorders. Using a candidate gene approach, we identified nonambiguous mutations in NALP12 (i.e., nonsense and splice site) in two families with periodic fever syndromes. As shown by means of functional studies, these two NALP12 mutations have a deleterious effect on NF-kappaB signaling. Overall, these data identify a group of HPFs defined by molecular defects in NALP12, opening up new ways to manage these disorders. The identification of these first NALP12 mutations in patients with autoinflammatory disorder also clearly demonstrates the crucial role of NALP12 in inflammatory signaling pathways, thereby assigning a precise function to this particular member of an emerging family of proteins whose putative biological properties are currently inferred essentially through in vitro means.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U.654, Université Paris 6 Pierre et Marie Curie, 75012 Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Jéru
authors.forename: P
authors.initials: P
authors.lastname: Duquesnoy
authors.forename: T
authors.initials: T
authors.lastname: Fernandes-Alnemri
authors.forename: E
authors.initials: E
authors.lastname: Cochet
authors.forename: J W
authors.initials: JW
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Lackmy-Port-Lis
authors.forename: E
authors.initials: E
authors.lastname: Grimprel
authors.forename: J
authors.initials: J
authors.lastname: Landman-Parker
authors.forename: V
authors.initials: V
authors.lastname: Hentgen
authors.forename: S
authors.initials: S
authors.lastname: Marlin
authors.forename: K
authors.initials: K
authors.lastname: McElreavey
authors.forename: T
authors.initials: T
authors.lastname: Sarkisian
authors.forename: G
authors.initials: G
authors.lastname: Grateau
authors.forename: E S
authors.initials: ES
authors.lastname: Alnemri
authors.forename: S
authors.initials: S
authors.lastname: Amselem
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002648:Child; D018389:Codon, Nonsense / Q000235:genetics; D010505:Familial Mediterranean Fever / Q000235:genetics*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D016328:NF-kappa B / Q000378:metabolism; D010375:Pedigree; D022821:RNA Splice Sites; D012326:RNA Splicing / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D018389:Codon, Nonsense; D047908:Intracellular Signaling Peptides and Proteins; D016328:NF-kappa B; C476731:NLRP12 protein, human; D022821:RNA Splice Sites