Genomovar status, virulence markers and genotyping of Burkholderia cepacia complex strains isolated from Brazilian cystic fibrosis patients.
Burkholderia cepacia complex isolates obtained by microbiological culture of respiratory samples from Brazilian CF patients were studied by recA based PCR, screened by specific PCR for virulence markers and genotyped by RAPD. Forty-one isolates of B. cepacia complex were identified by culture and confirmation of identity and genomovar determination obtained in 32 isolates, with predominance of B. cenocepacia (53.1%). Virulence markers were not consistently found among isolates. Genotyping did not identify identical patterns among different patients. B. cenocepacia was the most prevalent B. cepacia complex member among our patients, and cross-infection does not seem to occur among them.
Martins
Kátia Maia
KM
Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil.
Fongaro
Giuliana F
GF
Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil.
Dutra Rodrigues
André B
AB
Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil.
Tateno
Adriana F
AF
Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil.
Azzuz-Chernishev
Ana Carolina
AC
Bacteriology Section, Adolfo Lutz Institute, São Paulo, Brazil.
Oliveira-Garcia
Doroti de
D
Bacteriology Section, Adolfo Lutz Institute, São Paulo, Brazil.
Rodrigues
Joaquim C
JC
Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil.
da Silva Filho
Luiz Vicente F
LVF
Pediatric Pulmonology Unit, Instituto da Criança, HC-FMUSP, São Paulo, Brazil; Laboratory of Virology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, São Paulo, Brazil. Electronic address: vicres@usp.br.
D001938:Brazil / Q000453:epidemiology; D019121:Burkholderia Infections / Q000453:epidemiology / Q000235:genetics*; D042602:Burkholderia cepacia complex / Q000235:genetics* / Q000302:isolation & purification*; D015331:Cohort Studies; D003550:Cystic Fibrosis / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D015995:Prevalence; D011938:Rec A Recombinases / Q000235:genetics; D014774:Virulence / Q000235:genetics
D011938:Rec A Recombinases