RecJ, ExoI and RecG are required for genome maintenance but not for generation of genetic diversity by repeat-mediated phase variation in Haemophilus influenzae.
High levels of genetic diversity are generated in Haemophilus influenzae populations through DNA repeat-mediated phase variation and recombination with DNA fragments acquired by uptake from the external milieu. Conversely, multiple pathways for maintenance of the genome sequence are encoded in H. influenzae genomes. In Escherichia coli, mutations in single-stranded-DNA exonucleases destabilise tandem DNA repeats whilst inactivation of recG can stabilise repeat tracts. These enzymes also have varying effects on recombination. Deletion mutations were constructed in H. influenzae genes encoding homologs of ExoI, RecJ and RecG whilst ExoVII was refractory to mutation. Inactivation of RecJ and RecG, but not ExoI, increased sensitivity to irradiation with ultraviolet light. An increase in spontaneous mutation rate was not observed in single mutants but only when both RecJ and ExoI were mutated. None of the single- or double-mutations increased or decreased the rates of slippage in tetranucleotide repeat tracts. Furthermore, the exonuclease mutants did not exhibit significant defects in horizontal gene transfer. We conclude that RecJ, ExoI and RecG are required for maintenance of the H. influenzae genome but none of these enzymes influence the generation of genetic diversity through mutations in the tetranucleotide repeat tracts of this species.
Kumar
Gaurav A
GA
Molecular Infectious Diseases Group, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, UK.
Woodhall
Mark R
MR
Hood
Derek W
DW
Moxon
E Richard
ER
Bayliss
Christopher D
CD
D001426:Bacterial Proteins / Q000235:genetics*; D004249:DNA Damage; D004265:DNA Helicases / Q000235:genetics*; D005090:Exodeoxyribonucleases / Q000235:genetics*; D022761:Gene Transfer, Horizontal; D014644:Genetic Variation; D042822:Genomic Instability; D006193:Haemophilus influenzae / Q000235:genetics* / Q000254:growth & development; D016296:Mutagenesis; D017384:Sequence Deletion; D020080:Tandem Repeat Sequences
D001426:Bacterial Proteins; D005090:Exodeoxyribonucleases; C070765:recJ protein, Bacteria; C028450:exodeoxyribonuclease I; D004265:DNA Helicases