title: Cinnamic acid, an autoinducer of its own biosynthesis, is processed via Hca enzymes in Photorhabdus luminescens.
abstract: Photorhabdus luminescens, an entomopathogenic bacterium and nematode symbiont, has homologues of the Hca and Mhp enzymes. In Escherichia coli, these enzymes catalyze the degradation of the aromatic compounds 3-phenylpropionate (3PP) and cinnamic acid (CA) and allow the use of 3PP as sole carbon source. P. luminescens is not able to use 3PP and CA as sole carbon sources but can degrade them. Hca dioxygenase is involved in this degradation pathway. P. luminescens synthesizes CA from phenylalanine via a phenylalanine ammonia-lyase (PAL) and degrades it via the not-yet-characterized biosynthetic pathway of 3,5-dihydroxy-4-isopropylstilbene (ST) antibiotic. CA induces its own synthesis by enhancing the expression of the stlA gene that codes for PAL. P. luminescens bacteria release endogenous CA into the medium at the end of exponential growth and then consume it. Hca dioxygenase is involved in the consumption of endogenous CA but is not required for ST production. This suggests that CA is consumed via at least two separate pathways in P. luminescens: the biosynthesis of ST and a pathway involving the Hca and Mhp enzymes.
authors.affiliation: Unité de Génétique des Membranes Bactériennes, CNRS URA 2172, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France.
authors.forename: Sabina
authors.initials: S
authors.lastname: Chalabaev
authors.forename: Evelyne
authors.initials: E
authors.lastname: Turlin
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bay
authors.forename: Christelle
authors.initials: C
authors.lastname: Ganneau
authors.forename: Emma
authors.initials: E
authors.lastname: Brito-Fravallo
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Charles
authors.forename: Antoine
authors.initials: A
authors.lastname: Danchin
authors.forename: Francis
authors.initials: F
authors.lastname: Biville
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000737:chemistry / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D008954:Models, Biological; D008957:Models, Genetic; D015394:Molecular Structure; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D010666:Phenylpropionates / Q000737:chemistry / Q000378:metabolism; D020637:Photorhabdus / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D002934:Cinnamates; D010666:Phenylpropionates; C029010:cinnamic acid; D010650:Phenylalanine Ammonia-Lyase