Isolation of T-tubules from skeletal muscle.
The transverse tubules (T-tubules) of mammalian cardiac and skeletal muscles are invaginations of the sarcolemma. They play a crucial role in excitation-contraction coupling as well as in intracellular signaling and in regulation of glucose transport. The biochemical purification of T-tubule membranes is a difficult task, and membrane fractions enriched in transverse tubules are usually contaminated with other cell-surface and intracellular membranes. This unit includes methods that permit the isolation and purification of T-tubules from skeletal muscle.
Zorzano
Antonio
A
Departament de Bioquimica i Biologia, Molecular, Universitat de Barcelona, and Institute for Research in Biomedicine, Barcelona Science Park, Barcelona, Spain.
Camps
Marta
M
D000818:Animals; D019411:Clinical Laboratory Techniques; D008297:Male; D018482:Muscle, Skeletal / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure