title: In vitro optimisation of topical negative pressure regimens for angiogenesis into synthetic dermal replacements.
abstract: BACKGROUND
  The use of synthetic dermal replacements (SDRs) in the treatment of large wounds, which have associated morbidity and mortality, has attracted great interest. However, because of poor outcome, SDRs have limited use. The addition of topical negative pressure (TNP) has increased their success, but little research has focused on the underlying mechanisms. This paper studies the in vitro effects of TNP on commonly used SDRs to identify the most effective TNP regimen and optimum SDR for encouraging endothelial cell ingress.
  
  
  METHODS
  Endothelial cells were co-cultured in vitro on four SDRs with or without TNP. Negative pressure (125mmHg) was applied intermittently, continuously, for 4h per day, or not at all. Endothelial ingress was measured for each condition.
  
  
  RESULTS
  In the collagen controls, cell migration was minimal. Integratrade mark gave the greatest endothelial cell migration (p<0.05, n=3). TNP increased endothelial cell migration, intermittent application being the optimum regimen.
  
  
  CONCLUSIONS
  Integratrade mark has an open sponge structure which may account for greater angiogenicity than Allodermtrade mark, Permacoltrade mark and Xenodermtrade mark. In vitro intermittent TNP stimulates the greatest angiogenic response. The majority of clinical studies investigating SDR success with TNP have used continuous regimens; this study suggests a change in clinical practice to intermittent application.
authors.affiliation: RAFT Institute, Mount Vernon Hospital, Northwood, London, UK. Mattpotters@hotmail.com
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Potter
authors.forename: Paul
authors.initials: P
authors.lastname: Banwell
authors.forename: Christopher
authors.initials: C
authors.lastname: Baldwin
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Clayton
authors.forename: Laurie
authors.initials: L
authors.lastname: Irvine
authors.forename: Claire
authors.initials: C
authors.lastname: Linge
authors.forename: Addrian O
authors.initials: AO
authors.lastname: Grobbelaar
authors.forename: Roy
authors.initials: R
authors.lastname: Sanders
authors.forename: Julian F
authors.initials: JF
authors.lastname: Dye
mesh_terms: D002056:Burns / Q000628:therapy*; D020405:Dermis / Q000098:blood supply*; D006801:Humans; D054843:Negative-Pressure Wound Therapy / Q000379:methods*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D017192:Skin Diseases, Bacterial / Q000517:prevention & control; D017768:Skin, Artificial; D013314:Stress, Mechanical