[Mitochondrial DNA A1555G mutation analysis in 802 nonsyndromic hearing impairment patients].
OBJECTIVE
To investigate the prevalence of the mitochondrial DNA (mtDNA) A1555G mutation in nonsyndromic hearing impairment (NSHI) patients from Gansu province.


METHODS
Subjects included 802 students selected from five Deaf-Mute Schools in Gansu. DNA was extracted from peripheral blood of all patients. The mitochondrial DNA target fragments were amplified by polymerase chain reaction (PCR). The Mutations were detected by AIw26I digestion and sequence analysis.


RESULTS
The homoplasmic A1555G mutation was found in 67 individuals from 802 patients (8.4%). Fifteen of these 67 patients had family histories.


CONCLUSIONS
The mtDNA A1555G mutation had a higher incidence in Gansu population with nonsyndromic hearing impairment than other studies. The data not only gaven more evidences that the prevalence of mtDNA A1555G mutation in china was higher than that in Europe and America, but also gaven valuable information for gene diagnosis, genetic counseling and would improve the safety of aminoglycoside antibiotic therapy.
Liu
Xiao-wen
XW
Department of Otorhinolaryngology Head Neck Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, China.
Guo
Yu-fen
YF
Han
Dong-yi
DY
Zhao
Ya-li
YL
Lan
Lan
L
Zhao
Cui
C
Wang
Qiu-ju
QJ
D000293:Adolescent; D044466:Asian People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D055815:Young Adult
D004272:DNA, Mitochondrial