Hypericum perforatum plant cells reduce Agrobacterium viability during co-cultivation.
Plant recalcitrance is the major barrier in developing Agrobacterium-mediated transformation protocols for several important plant species. Despite the substantial knowledge of T-DNA transfer process, very little is known about the factors leading to the plant recalcitrance. Here, we analyzed the basis of Hypericum perforatum L. (HP) recalcitrance to Agrobacterium-mediated transformation using cell suspension culture. When challenged with Agrobacterium, HP cells swiftly produced an intense oxidative burst, a typical reaction of plant defense. Agrobacterium viability started to decline and reached 99% mortality within 12 h, while the plant cells did not suffer apoptotic process. This is the first evidence showing that the reduction of Agrobacterium viability during co-cultivation with recalcitrant plant cells can affect transformation.
Franklin
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G
Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Conceição
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Kombrink
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Dias
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D016960:Agrobacterium tumefaciens / Q000166:cytology* / Q000502:physiology; D015152:Blotting, Northern; D002470:Cell Survival; D018744:DNA, Plant / Q000235:genetics; D020902:Hypericum / Q000235:genetics / Q000382:microbiology / Q000502:physiology*; D007700:Kinetics; D017382:Reactive Oxygen Species / Q000378:metabolism
D018744:DNA, Plant; D017382:Reactive Oxygen Species