Injectable and sustained delivery of human growth hormone using chemically modified Pluronic copolymer hydrogels.
A new injectable biodegradable hydrogel system with thermosensitive sol-gel transition behavior was developed. A series of A-B-A triblock copolymers consisting of Pluronic copolymer end-capped with D- or L-lactic acid oligomers (PL-LA(n)) with various chain lengths (n = 5,12) was synthesized. It was assumed that a pair of two triblock copolymers with enantiomeric oligolactide chains, when blended in an equimolar mixture, would form more stable, self-assembled, and stereocomplexed (ST) hydrogels. A series of blend hydrogels encapsulating human growth hormone (hGH) was prepared by varying blend ratios between PL and stereocomplexed PL copolymers. They showed sustained release of hGH via an erosion-dependent mechanism. The hydrogel with a 5% blending ratio exhibited the most delayed mass erosion as well as sustained protein release patterns in vitro possibly due to the formation of a fish-net like 3-D mesh structure. The effect of incubation condition on hGH release and degradation behaviors was also assessed.
Park
Sung Young
SY
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
Chung
Hyun Jung
HJ
Lee
Yuhan
Y
Park
Tae Gwan
TG
D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry*; D004058:Diffusion; D004353:Drug Evaluation, Preclinical; D019382:Human Growth Hormone / Q000008:administration & dosage* / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D008422:Materials Testing; D020442:Poloxamer / Q000737:chemistry*
D003692:Delayed-Action Preparations; D020100:Hydrogels; D020442:Poloxamer; D019382:Human Growth Hormone