title: Magnetic particle-based sandwich sensor with DNA-modified carbon nanotubes as recognition elements for detection of DNA hybridization.
abstract: In this contribution, we design a visual sensor for DNA hybridization with DNA probe-modified magnetic particles (MPs) and multiwalled carbon nanotubes (MWNTs) without involving a visual recognition element such as fluorescent/chemiluminescent reagents. It was found that DNA probe-modified MWNTs, which could be dispersed in aqueous medium and have strong light scattering signals under the excitation of a light beam in the UV-vis region, could connect with DNA probe-modified MPs together in the presence of perfectly complementary target DNA and form a sandwich structure. In a magnetic field, the formed MP-MWNT species can easily be removed from the solution, resulting in a decrease of light scattering signals. Thus, a magnetic particle-based sandwich sensor could be developed to detect DNA hybridization by measuring the light scattering signals with DNA-modified MWNTs as recognition elements. Experiments showed that the DNA-modified MPs sensor could be reused at least 17 times and was stable for more than 6 months.
authors.affiliation: College of Chemistry and Chemical Engineering, CQKL-LRTA, College of Life Sciences, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Po
authors.initials: P
authors.lastname: Hu
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
authors.forename: Yuan Fang
authors.initials: YF
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Ling
authors.forename: Yu Ling
authors.initials: YL
authors.lastname: Liu
authors.forename: Liang Run
authors.initials: LR
authors.lastname: Fei
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Xie
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D015342:DNA Probes / Q000737:chemistry*; D008280:Magnetics; D037742:Nanotubes, Carbon / Q000737:chemistry*; D045162:Thiazolidinediones / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D037742:Nanotubes, Carbon; D045162:Thiazolidinediones; C114240:DN 108