Single-walled carbon nanotubes can induce pulmonary injury in mouse model.
Carbon nanotubes are a nanomaterial that is extensively used in industry. The potential health risk of chronic carbon nanotubes exposure has been raised as of great public concern. In the present study, we have demonstrated that intratracheal instillation of 0.5 mg of single-walled carbon nanotubes (SWCNT) into male ICR mice (8 weeks old) induced alveolar macrophage activation, various chronic inflammatory responses, and severe pulmonary granuloma formation. We then used Affymetrix microarrays to investigate the molecular effects on the macrophages when exposed to SWCNT. A biological pathway analysis, a literature survey, and experimental validation suggest that the uptake of SWCNT into the macrophages is able to activate various transcription factors such as nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1), and this leads to oxidative stress, the release of proinflammatory cytokines, the recruitment of leukocytes, the induction of protective and antiapoptotic gene expression, and the activation of T cells. The resulting innate and adaptive immune responses may explain the chronic pulmonary inflammation and granuloma formation in vivo caused by SWCNT.
Chou
Cheng-Chung
CC
Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi, Taiwan 621, ROC.
Hsiao
Hsiang-Yun
HY
Hong
Qi-Sheng
QS
Chen
Chun-Houh
CH
Peng
Ya-Wen
YW
Chen
Huei-Wen
HW
Yang
Pan-Chyr
PC
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D008168:Lung / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008171:Lung Diseases / Q000139:chemically induced* / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D037742:Nanotubes, Carbon / Q000633:toxicity* / Q000648:ultrastructure
D016207:Cytokines; D037742:Nanotubes, Carbon