Regulation of host cell cyclin D1 by Trypanosoma cruzi in myoblasts.
Infection with the parasite Trypanosoma cruzi causes Chagas disease. In this study we demonstrated that there was an increase in cyclin D1 expression in T. cruzi (Tulahuen strain)-infected myoblasts. To examine a possible mechanism for the increased cyclin D1 expression we transfected L(6)E(9) myoblasts with cyclin D1 luciferase reporter constructs and infected with T. cruzi. There was no evidence of an increase in promoter activity. Additionally, quantitative PCR did not demonstrate any change in cyclin D1 message during infection. Moreover, we demonstrated that the cyclin D1 protein was significantly stabilized after infection. Collectively, these data indicate that infection with T. cruzi increases cyclin D1 protein abundance post-translationally.
Bouzahzah
Boumediene
B
Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Yurchenko
Vyacheslav
V
Nagajyothi
Fnu
F
Hulit
James
J
Sadofsky
Moshe
M
Braunstein
Vicki L
VL
Mukherjee
Shankar
S
Weiss
Hannah
H
Machado
Fabiana S
FS
Pestell
Richard G
RG
Lisanti
Michael P
MP
Tanowitz
Herbert B
HB
Albanese
Chris
C
D000818:Animals; D014355:Chagas Disease / Q000378:metabolism*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008156:Luciferases; D032446:Myoblasts / Q000378:metabolism* / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000378:metabolism*
D017931:DNA Primers; D019938:Cyclin D1; D008156:Luciferases