title: Cytotoxic activity of an octadecenoic acid extract from Euphorbia kansui (Euphorbiaceae) on human tumour cell strains.
abstract: We have investigated the cytotoxic and antitumour activity of an octadecenoic acid extract, mainly containing oleic and linoleic acids, from Euphorbia kansui on human gastric (SGC-7901), hepatocellular carcinoma (BEL-7402), and leukaemia (HL-60) tumour cell strains. Significant and dose-dependent antiproliferation effects were observed on tumour cells from the dose of 3.2 microg mL(-1), which were comparable with or better than those of the common antitumour agent 5-fluorouracil. Results from the clone formation assay and flow cytometry indicated that the mixture of octadecenoic acids resulted in a dose-dependent reduction in the number of tumour cells and significantly inhibited cell proliferation, with induced apoptosis and G(0)/G(1) phase cell cycle arrest. Also, the octadecenoic acids could not only cause cell apoptosis/necrosis but also functionally and structurally damage the tumour cell membrane and cell ultra-structures. These observations encourage further clinical evaluation of the inhibitory effects of octadecenoic acids on various forms of cancer.
authors.affiliation: School of Public Security, Gansu Institute of Political Science and Law, Lanzhou 730070, China. fyu@ufl.edu
authors.forename: Farong
authors.initials: F
authors.lastname: Yu
authors.forename: Shunqing
authors.initials: S
authors.lastname: Lu
authors.forename: Fahong
authors.initials: F
authors.lastname: Yu
authors.forename: Junnian
authors.initials: J
authors.lastname: Shi
authors.forename: Peter M
authors.initials: PM
authors.lastname: McGuire
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D028481:Euphorbia / Q000737:chemistry*; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D008041:Linoleic Acids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D009829:Oleic Acids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D008041:Linoleic Acids; D009829:Oleic Acids; D010936:Plant Extracts; D005472:Fluorouracil