Editing through multiple bonds: threonine detection.
In in vivo (1)H spectroscopy, the signal at 1.32 ppm is usually assigned to lactate. This resonance position is shared with threonine at physiological pH. The similarity of spectral patterns of lactate and threonine renders the separate measurement of either threonine or lactate without and even with editing technically challenging. In this study, the threonine signal was detected using a single-shot multiple-bond editing technique and quantified in vivo in both rat and human brains. A threonine concentration was estimated at 0.8 +/- 0.3 mM (mean +/- SD, n = 6) in the rat brain and at approximately 0.33 mM in the human brain.
Marjanska
Malgorzata
M
Center for Magnetic Resonance Research and Department of Radiology, University of Minnesota, Minneapolis, MN 55455, USA. gosia@cmrr.umn.edu
Henry
Pierre-Gilles
PG
Uğurbil
Kâmil
K
Gruetter
Rolf
R
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D019047:Phantoms, Imaging; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013912:Threonine / Q000378:metabolism*
D007773:Lactates; D013912:Threonine