title: Interaction trap/two-hybrid system to identify interacting proteins.
abstract: This unit presents protocols designed to detect interacting proteins. Using yeast as a "test tube" and transcriptional activation of a reporter system, interacting proteins can be identified. The system can also be used to test complex formation for proteins for which there exists a reason to expect interaction.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Golemis
authors.forename: I
authors.initials: I
authors.lastname: Serebriiskii
authors.forename: R L
authors.initials: RL
authors.lastname: Finley
authors.forename: M G
authors.initials: MG
authors.lastname: Kolonin
authors.forename: J
authors.initials: J
authors.lastname: Gyuris
authors.forename: R
authors.initials: R
authors.lastname: Brent
mesh_terms: D000818:Animals; D015723:Gene Library; D006801:Humans; D010957:Plasmids; D011506:Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D015003:Yeasts
chemical_list: D011506:Proteins