The colorimetric detection and quantitation of total protein.
Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. Colorimetric methods are fast, simple, and not laborious. This unit describes a number of assays able to detect protein concentrations in the low microgram to milligram per milliliter ranges in a variety of formats.
Krohn
Randall I
RI
Pierce Chemical, Rockford, Illinois, USA.
D001738:Biuret Reaction / Q000379:methods; D002138:Calibration / Q000592:standards; D003124:Colorimetry / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D011506:Proteins / Q000032:analysis*; D011804:Quinolines / Q000737:chemistry; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000592:standards
D011506:Proteins; D011804:Quinolines; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine; C047117:bicinchoninic acid; C004692:coomassie Brilliant Blue