[A static magnetic field loading system for in vitro cultured cells].
Magnetic attachments have flux leakages, thus they will exert certain magnetic fields on the adjacent tissues when used in the patients' oral cavities. There are few research reports on the biological effects of the magnetic fields generated by magnetic attachments on human body. A cellular static magnetic field (SMF) loading system was developed in this study. By using this system, in vitro cultured human periodontal ligament cells (HPDLCs) were loaded with SMF simulating those of magnetic attachments. The cellular SMF loading system could produce constant SMF and the strength of the SMF is adjustable. The system is small and is able to exert SMF to cells cultured in different culture vessels such as culture dishes and culture plates, thus is suitable to researches in multiple biological items of cells. The results of the SMF loading experiment on HPDLCs showed that this cellular SMF loading system could effectively load cells with SMF of different strengths for different time in vitro. The development of this system has provided a useful tool for the researches on the cellular hiologioal effects of SMF.
Xu
Chun
C
Department of Prosthodontics, Affiliated Ninth People's Hospital, Medical College, Shanghai Jiaotong University/Shanghai Research Institute of Stomatology, Shanghai 200011, China. imxuchun@163.com
Chao
Yonglie
Y
Fan
Zhen
Z
Du
Li
L
Zhang
Fuqiang
F
D002478:Cells, Cultured; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D008280:Magnetics; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*