title: Expressed sequence tags (ESTs) from the salivary glands of the tick Amblyomma cajennense (Acari: Ixodidae).
abstract: The neotropical tick Amblyomma cajennense is a significant pest to domestic animals, the most frequently human-biting tick in South America and the main vector of Brazilian spotted fever (caused by Rickettsia rickettsii), a deadly human disease. The purpose of this study is to characterize the adult A. cajennense salivary gland transcriptome by expressed sequence tags (ESTs). We report the analysis of 1754 clones obtained from a cDNA library, which reveal mainly transcripts related to proteins involved in the hemostatic processes, especially proteases and their inhibitors. Remarkably, five types of possible serine protease inhibitors were found, including a molecule with a distinguished structure that contains repeats of the active motif of hirudin inhibitors. Besides, other components that may be active over the host immune system or acting as defensins against infecting microorganisms were also described, including a molecule similar to insect venom allergens. The conjunction of components from this transcriptome suggests a diverse strategy of A. cajennense tick during feeding, but emphasized in the coagulation system.
authors.affiliation: Laboratório de Bioquímica e Biofísica-Instituto Butantan, Av. Vital Brazil, 1500, CEP 05503-900, Sâo Paulo, SP, Brazil.
authors.forename: Isabel F C
authors.initials: IF
authors.lastname: Batista
authors.forename: Ana M
authors.initials: AM
authors.lastname: Chudzinski-Tavassi
authors.forename: Fernanda
authors.initials: F
authors.lastname: Faria
authors.forename: Simone M
authors.initials: SM
authors.lastname: Simons
authors.forename: Darci M
authors.initials: DM
authors.lastname: Barros-Batestti
authors.forename: Marcelo B
authors.initials: MB
authors.lastname: Labruna
authors.forename: Luciana I
authors.initials: LI
authors.lastname: Leão
authors.forename: Paulo L
authors.initials: PL
authors.lastname: Ho
authors.forename: Inácio L M
authors.initials: IL
authors.lastname: Junqueira-de-Azevedo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001096:Arachnid Vectors / Q000378:metabolism*; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D026863:Ixodidae / Q000378:metabolism*; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
chemical_list: D012471:Salivary Proteins and Peptides; D010447:Peptide Hydrolases