title: Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation.
abstract: Microspheres have been prepared from the resorbable linear polyester of beta-hydroxybutyric acid (polyhydroxybutyrate, PHB) by the solvent evaporation technique and investigated in vitro and in vivo. Biocompatibility of the microspheres has been proved in tests in the culture of mouse fibroblast cell line NIH 3T3 and in experiments on intramuscular implantation of the microspheres to Wistar rats for 3 months. Tissue response to the implantation of polymeric microspheres has been found to consist in a mild inflammatory reaction, pronounced macrophage infiltration that increases over time, involving mono- and poly-nuclear foreign body giant cells that resorb the polymeric matrix. No fibrous capsules were formed around polymeric microparticles; neither necrosis nor any other adverse morphological changes and tissue transformation in response to the implantation of the PHB microparticles were recorded. The results of the study suggest that polyhydroxybutyrate is a good candidate for fabricating prolonged-action drugs in the form of microparticles intended for intramuscular injection.
authors.affiliation: Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics SB RAS (Siberian Branch Russian Academy of Sciences), Akademgorodok, 50, Krasnoyarsk 660036, Russia. shishatskaya@inbox.ru
authors.forename: Ekaterina Igorevna
authors.initials: EI
authors.lastname: Shishatskaya
authors.forename: Olga N
authors.initials: ON
authors.lastname: Voinova
authors.forename: Anastasiya V
authors.initials: AV
authors.lastname: Goreva
authors.forename: Olga A
authors.initials: OA
authors.lastname: Mogilnaya
authors.forename: Tatiana G
authors.initials: TG
authors.lastname: Volova
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002470:Cell Survival; D066298:In Vitro Techniques; D007249:Inflammation; D008422:Materials Testing; D051379:Mice; D008863:Microspheres; D041681:NIH 3T3 Cells; D009336:Necrosis; D011108:Polymers / Q000737:chemistry; D000091402:Prohibitins; D051381:Rats; D017208:Rats, Wistar; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; C000717332:Phb protein, rat; D011108:Polymers; D000091402:Prohibitins; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D020155:3-Hydroxybutyric Acid