CXCR3 mediates region-specific antiviral T cell trafficking within the central nervous system during West Nile virus encephalitis.
Regional differences in inflammation during viral infections of the CNS suggest viruses differentially induce patterns of chemoattractant expression, depending on their cellular targets. Previous studies have shown that expression of the chemokine CXCL10 by West Nile virus (WNV)-infected neurons is essential for the recruitment of CD8 T cells for the purpose of viral clearance within the CNS. In the current study we used mice deficient for the CXCL10 receptor, CXCR3, to evaluate its role in leukocyte-mediated viral clearance of WNV infection within various CNS compartments. WNV-infected CXCR3-deficient mice exhibited significantly enhanced mortality compared with wild-type controls. Immunologic and virologic analyses revealed that CXCR3 was dispensable for control of viral infection in the periphery and in most CNS compartments but, surprisingly, was required for CD8 T cell-mediated antiviral responses specifically within the cerebellum. WNV-specific, CXCR3-expressing T cells preferentially migrated into the cerebellum, and WNV-infected cerebellar granule cell neurons expressed higher levels of CXCL10 compared with similarly infected cortical neurons. These results indicate that WNV differentially induces CXCL10 within neuronal populations and suggest a novel model for nonredundancy in chemokine-mediated inflammation among CNS compartments.
Zhang
Bo
B
Division of Infectious Diseases, Children's Hospital of Boston, Boston, MA, USA.
Chan
Ying Kai
YK
Lu
Bao
B
Diamond
Michael S
MS
Klein
Robyn S
RS
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000276:immunology* / Q000821:virology; D054357:Chemokine CXCL10 / Q000172:deficiency / Q000235:genetics / Q000502:physiology; D054370:Chemokine CXCL9 / Q000172:deficiency / Q000235:genetics; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D018792:Encephalitis, Viral / Q000276:immunology* / Q000401:mortality / Q000473:pathology / Q000821:virology; D005260:Female; D015967:Gene Expression Regulation, Viral / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054367:Receptors, CXCR3 / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D019562:Viral Load; D014901:West Nile Fever / Q000276:immunology* / Q000401:mortality / Q000821:virology; D014902:West Nile virus / Q000276:immunology
D054357:Chemokine CXCL10; D054370:Chemokine CXCL9; C485433:Cxcl10 protein, mouse; C485969:Cxcl9 protein, mouse; C515188:Cxcr3 protein, mouse; D054367:Receptors, CXCR3