title: Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated.
abstract: We investigated the presence of glycoproteins in Borrelia burgdorferi. We did not find any evidence for glycosylation of the major outer membrane proteins OspA and OspB or the structural flagellar proteins FlaB and FlaA. We suggest that glycoproteins present on the surface of B. burgdorferi may be tightly bound culture medium glycoproteins.
authors.affiliation: Biology Center of Academy of Sciences of the Czech Republic, Institute of Parasitology, Branisovská 31, CZ-37005 Ceské Budejovice, Czech Republic. sterbaj@paru.cas.cz
authors.forename: Ján
authors.initials: J
authors.lastname: Sterba
authors.forename: Marie
authors.initials: M
authors.lastname: Vancová
authors.forename: Nataliia
authors.initials: N
authors.lastname: Rudenko
authors.forename: Maryna
authors.initials: M
authors.lastname: Golovchenko
authors.forename: Tammy-Lynn
authors.initials: TL
authors.lastname: Tremblay
authors.forename: John F
authors.initials: JF
authors.lastname: Kelly
authors.forename: C Roger
authors.initials: CR
authors.lastname: MacKenzie
authors.forename: Susan M
authors.initials: SM
authors.lastname: Logan
authors.forename: Libor
authors.initials: L
authors.lastname: Grubhoffer
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000235:genetics / Q000378:metabolism; D025065:Borrelia burgdorferi / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D004591:Electrophoresis, Polyacrylamide Gel; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; C072045:OspA protein; D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin; C077030:OspB protein, Borrelia burgdorferi