Bioremediation potential of basidiomycetes isolated from compost.
The potential of a consortium of three basidiomycete mycelia isolated from compost to degrade polycyclic aromatic hydrocarbons (PAH) was first evaluated using a test based on decolorization of Poly R-478 dye. When pre-grown on straw, the consortium decolorized the dye by 83% in 7 days and generated a laccase activity of 663 IU l(-1). Its ability to degrade naphthalene was investigated in soil microcosms specially suited for this volatile PAH. The kinetic study was conducted at a maximal naphthalene concentration of 500 mg kg(-1) of soil. Naphthalene concentration, CO(2) evolution and phytotoxicity (germination index, GI%) on Lepidium sativum seeds were monitored. The naphthalene concentration decreased by about 70% in three weeks in the presence of metabolic activity, while the GI% increased indicating reduced phytotoxicity.
Anastasi
Antonella
A
Dipartimento di Biologia Vegetale, Università degli Studi di Torino, Viale Mattioli 25, 10125 Torino, Italy.
Varese
Giovanna C
GC
Bosco
Francesca
F
Chimirri
Fabiana
F
Marchisio
Valeria Filipello
VF
D001487:Basidiomycota / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D003116:Color; D052918:Environmental Restoration and Remediation / Q000379:methods*; D007700:Kinetics; D042845:Laccase / Q000378:metabolism; D031220:Lepidium sativum / Q000196:embryology; D009281:Naphthalenes / Q000032:analysis / Q000633:toxicity; D011083:Polycyclic Compounds / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects; D012987:Soil; D014835:Volatilization
D009281:Naphthalenes; D011083:Polycyclic Compounds; D012987:Soil; C031721:naphthalene; D042845:Laccase