Structural characterization of the cell wall D-glucans isolated from the mycelium of Botryosphaeria rhodina MAMB-05.
Three D-glucans were isolated from the mycelium of the fungus Botryosphaeria rhodina MAMB-05 by sequential extraction with hot-water and hot aqueous KOH (2% w/v) followed by ethanol precipitation. Following their purification by gel permeation chromatography on Sepharose CL-4B, the structural characteristics of the D-glucans were determined by FT-IR and 13C NMR spectroscopy and, after methylation, by GC-MS. The hot-water extract produced a fraction designated Q1A that was a beta-(1-->6)-D-glucan with the following structure: [Formula: see text] The alkaline extract, when subjected to repeated freeze-thawing, yielded two fractions: K1P (insoluble) that comprised a beta-(1-->3)-D-glucan with beta-D-glucose branches at C-6 with the structure: [Formula: see text] and K1SA (soluble) consisting of a backbone chain of alpha-(1-->4)-linked D-glucopyranosyl residues substituted at O-6 with alpha-D-glucopyranosyl residues: [Formula: see text]
de Lourdes Corradi da Silva
Maria
M
Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CP 467, CEP 19060-900, Presidente Prudente, São Paulo, Brazil. corradi@fct.unesp.br
Fukuda
Eliane K
EK
Vasconcelos
Ana Flora D
AF
Dekker
Robert F H
RF
Matias
Andreza C
AC
Monteiro
Nilson K
NK
Cardoso
Marilsa S
MS
Barbosa
Aneli M
AM
Silveira
Joana L M
JL
Sassaki
Guilherme L
GL
Carbonero
Elaine R
ER
D001203:Ascomycota / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D025282:Mycelium / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D005936:Glucans