Obesity promotes injury induced femoral artery thrombosis in mice.
An FeCl(3) induced femoral arterial thrombosis model was applied to lean (47+/-1.4 g) and obese (64+/-1.7 g) mice (Swiss genetic background) in order to study the relation between obesity and thrombotic risk. As compared to lean mice, obese mice showed a significantly shorter occlusion time (9.9+/-1.0 min versus 13+/-0.5 min; p=0.04) and lower total blood flow (37+/-7.3% versus 69+/-6.7%; p=0.008). A significant negative correlation was observed between body weight and both occlusion time (r=-0.57; p=0.014) and blood flow (r=-0.57; p=0.028). Analysis of the coagulation profile revealed significantly higher levels of plasminogen activator inhibitor-1 (PAI-1), thrombin-antithrombin complex, Factor V activity and combined Factors II/VII/X activity, and moderately elevated Factor VIII activity in obese mice. The degree of arterial damage and the thrombus extension were, however, not significantly different. A significant positive correlation was observed between body weight and either PAI-1 (r=0.63; p=0.003), Factors II/VII/X levels (r=0.80; p<0.0001) or Factor V levels (r=0.65; p=0.003). Thus, this injury induced femoral artery thrombosis model in mice establishes experimentally a correlation between obesity and prothrombotic tendency.
Nagai
Nobuo
N
Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium.
Hoylaerts
Marc F
MF
Cleuren
Audrey C A
AC
Van Vlijmen
Bart J M
BJ
Lijnen
H Roger
HR
D000818:Animals; D000991:Antithrombins / Q000378:metabolism; D001777:Blood Coagulation; D001835:Body Weight; D002712:Chlorides; D005165:Factor V / Q000378:metabolism; D005263:Femoral Artery / Q000473:pathology*; D005290:Ferric Compounds / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D015233:Models, Statistical; D009765:Obesity / Q000097:blood* / Q000150:complications*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D012306:Risk; D013927:Thrombosis / Q000097:blood* / Q000150:complications*
D000991:Antithrombins; D002712:Chlorides; D005290:Ferric Compounds; D017395:Plasminogen Activator Inhibitor 1; D005165:Factor V; C024555:ferric chloride