title: Inner membrane lipids of Escherichia coli form domains.
abstract: In prokaryotic cells, the hypothesis of the existence of lipid domains was considered. In order to test this hypothesis and study the organization of lipids in the inner membrane of Escherichia coli, we elaborated Langmuir films mimicking the inner leaflet of this membrane by considering lipids extracted from the inner membrane of E coli by Folch protocol. Lipid monolayers were elaborated by using these extracts (Langmuir technique); the organization of the resulting films was studied at the air-water interface by Brewster angle microscopy and after transfer onto muscovite by atomic force microscopy. The existence of domains was demonstrated for different interfacial pressures of biological interest, and their stability was studied.
authors.affiliation: Polymères, Biopolymères, Membranes, UMR 6522 CNRS, Université de Rouen, UFR des Sciences, 76821 Mont Saint Aignan Cedex, France.
authors.forename: Zakia
authors.initials: Z
authors.lastname: Zerrouk
authors.forename: Stéphane
authors.initials: S
authors.lastname: Alexandre
authors.forename: Céline
authors.initials: C
authors.lastname: Lafontaine
authors.forename: Vic
authors.initials: V
authors.lastname: Norris
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Valleton
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D008853:Microscopy / Q000379:methods; D018625:Microscopy, Atomic Force
chemical_list: D008563:Membrane Lipids