Function of the iron-binding chelator produced by Coriolus versicolor in lignin biodegradation.
An ultrafiltered low-molecular-weight preparation of chelating compounds was isolated from a wood-containing culture of the white-rot basidiomycete Coriolus versicolor. This preparation could chelate Fe3+ and reduce Fe3+ to Fe2+, demonstrating that the substance may serve as a ferric chelator, oxygen-reducing agent, and redox-cycling molecule, which would include functioning as the electron transport carrier in Fenton reaction. Lignin was treated with the iron-binding chelator and the changes in structure were investigated by 1H-NMR, 13C-NMR, difference spectrum caused by ionization under alkaline conditions and nitrobenzene oxidation. The results indicated that the iron-binding chelator could destroy the beta-O-4 bonds in etherified lignin units and insert phenolic hydroxyl groups. The low-molecular-weight chelator secreted by C. versicolor resulted in new phenolic substructures in the lignin polymer, making it susceptible to attack by laccase or manganese peroxidase. Thus, the synergic action of the iron-binding chelator and the lignocellulolytic enzymes made the substrate more accessible to degradation.
Wang
Lu
L
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Yan
WenChao
W
Chen
JiaChuan
J
Huang
Feng
F
Gao
PeiJi
P
D000447:Aldehydes / Q000737:chemistry; D005658:Fungi / Q000737:chemistry* / Q000378:metabolism*; D007501:Iron / Q000737:chemistry; D007502:Iron Chelating Agents / Q000737:chemistry* / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009578:Nitrobenzenes / Q000737:chemistry; D010084:Oxidation-Reduction; D019800:Phenol / Q000737:chemistry
D000447:Aldehydes; D007502:Iron Chelating Agents; D009578:Nitrobenzenes; D019800:Phenol; D008031:Lignin; D007501:Iron; C036077:nitrobenzene