Gene expression of Clonorchis sinensis metacercaria induced by gamma irradiation.
Gamma-rays are a form of ionizing radiation and produce serious cellular damage to nuclei and organelles. Gamma irradiation induces the expressions of genes involved in DNA repair. Clonorchis sinensis resides in and provokes pathophysiologic changes in the bile ducts of mammals. The C. sinensis metacercariae are unsusceptible or resistant to gamma irradiation with LD50 of 16.5 Gy. Using the annealing control primer-based polymerase chain reaction (PCR) method, 19 genes were found to be up-regulated in C. sinensis metacercariae exposed to gamma rays. Contigs of up-regulated genes (URGs) were retrieved in a C. sinensis expressed sequence tag pool and extended by DNA-walking. Of the 13 URGs annotated putatively as functional genes, five URGs were associated with energy metabolism, six with protein processing, and the other two with DNA repair protein RAD23 and inhibitor of apoptosis protein. Four URGs were confirmed up-regulated by gamma irradiation by quantitative real-time PCR. One unknown gene, which was up-regulated to the greatest extent, might contribute to early recovery from gamma-irradiation-induced damage. The up-regulations of genes encoding DNA repair, protein processing, and energy metabolism proteins suggests that increases in gene products orchestrate DNA lesion repair and recover cellular functions in gamma-irradiated C. sinensis metacercariae.
Kim
Tae Im
TI
Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul 156-756, Korea.
Cho
Pyo Yun
PY
Song
Kwang Jin
KJ
Li
Shunyu
S
Hong
Sung-Jong
SJ
Park
Suk Won
SW
Chai
Jong-Yil
JY
Shin
Eun-Hee
EH
D000818:Animals; D001483:Base Sequence; D003004:Clonorchis sinensis / Q000235:genetics* / Q000528:radiation effects*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D020224:Expressed Sequence Tags; D005720:Gamma Rays; D020869:Gene Expression Profiling; D015723:Gene Library; D017238:Genes, Helminth; D007928:Lethal Dose 50; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D015854:Up-Regulation
D018509:DNA, Helminth