Inhibitory effect of triclosan and nonylphenol on respiration rates and ammonia removal in activated sludge systems.
The toxic effects of triclosan (TCS) and nonylphenol (4-n-NP) on activated sludge heterotrophic and autotrophic microorganisms were evaluated. Toxicity experiments with specific oxygen uptake rate (SOUR) and ammonia uptake rate (AUR) revealed that TCS was much more toxic to heterotrophic and autotrophic microorganisms than 4-n-NP. In experiments with heterotrophic biomass, increase of sludge age (theta(c)) from 5 to 15 days resulted in a decrease of median effective concentrations (EC(50)) of TCS from 38.2 to 9.97 mg l(-1) and in an increase of EC(50) values of 4-n-NP from 441 to 649 mg l(-1). In experiments with autotrophic biomass and sludge age of 15 days, significantly lower EC(50) values were obtained for both compounds, indicating the higher sensitivity of nitrifiers to TCS and 4-n-NP. To compare toxicity of TCS and 4-n-NP towards single species and mixed wastewater cultures, experiments were performed using marine bacterium Vibrio fischeri. EC(50) values of 0.22 and 3.51 mg l(-1) were estimated for TCS and 4-n-NP, respectively, indicating the higher sensitivity of this bioassay to toxicants. According to the levels of tested compounds commonly found in influent wastewater and the results of this study, there is a possible risk for deterioration of nitrification in activated sludge systems due to the presence of TCS.
Stasinakis
Athanasios S
AS
Department of Environment, Water and Air Quality Laboratory, University of the Aegean, University Hill, Mytilene 81100, Greece. astas@env.aegean.gr
Mamais
Daniel
D
Thomaidis
Nikolaos S
NS
Danika
Elena
E
Gatidou
Georgia
G
Lekkas
Themistokles D
TD
D048908:Aliivibrio fischeri / Q000187:drug effects / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000891:Anti-Infective Agents, Local / Q000633:toxicity*; D018533:Biomass; D019149:Bioreactors; D049449:Luminescence; D010636:Phenols / Q000633:toxicity*; D012722:Sewage / Q000382:microbiology*; D014260:Triclosan / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000891:Anti-Infective Agents, Local; D010636:Phenols; D012722:Sewage; D014874:Water Pollutants, Chemical; D014260:Triclosan; D000641:Ammonia; C025256:nonylphenol