Trichostatin A and nuclear reprogramming of cloned rabbit embryos.
To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.
Shi
L H
LH
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, China.
Ai
J S
JS
Ouyang
Y C
YC
Huang
J C
JC
Lei
Z L
ZL
Wang
Q
Q
Yin
S
S
Han
Z M
ZM
Sun
Q Y
QY
Chen
D Y
DY
D000107:Acetylation; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D019976:Cloning, Organism; D004622:Embryo, Mammalian / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D056572:Histone Deacetylase Inhibitors; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011817:Rabbits / Q000196:embryology*
D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; D008239:Lysine