[Expression of the zinc finger transcriptional factor Snail in colorectal carcinoma and its significance].
OBJECTIVE
To study expression of the zinc finger transcriptional factor Snail in colorectal carcinoma and its significance.


METHODS
Expressions of Snail in colorectal carcinoma SW480 and SW620 cells were assayed by immunocytochemistry and immunofluorescent cytochemistry. The paraffin-embedded specimens from 68 cases of colorectal carcinoma and its corresponding adjacent tissues, 33 cases of adenoma and 35 cases of metastatic lymph nodes were also examined for Snail expressions using immunohistochemistry.


RESULTS
Snail protein was located mainly in the cell nucleus of SW480 and SW620 cells. The expressions of PRL-3 protein in the specimens of colorectal carcinoma, normal mucosa, adenoma and metastatic lymph nodes were significantly different (Chi(2)=92.852, P=0.000). In the adenoma tissues, the expression was significantly higher than that in normal mucosa (Z=-2.902, P=0.004), the metastatic lymphnodes had significantly higher expressions than the primary colorectal carcinomas (Z=-4.951, P=0.000), which, in turn, showed significantly higher expression than the adenoma tissues (Z=-3.572, P=0.000). Significant correlation of Snail expression was found to the progression and metastasis of colorectal carcinoma (Z=-2.043, P=0.041).


CONCLUSIONS
The expression of Snail is significantly correlated to genesis, progression and metastasis of colorectal carcinoma.
Gao
Wei-zhe
WZ
Department of Pathology, Nanfang Hospital/Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Ministry of Education/Guangdong Province, Southern Medical University, Guangzhou 510515, China. gzhgwz@126.com
Li
Jian-ming
JM
Yang
Fa-da
FD
Zhou
Jun
J
Ding
Yan-qing
YQ
D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000378:metabolism*; D016335:Zinc Fingers
D000071250:Snail Family Transcription Factors; D014157:Transcription Factors