Investigation of NAD(P)H:quinone oxidoreductase 1 (NQO1) C609T polymorphism in prostate cancer.
BACKGROUND
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a part of the antioxidant defense system. NQO1 protects cells from oxidative stress by maintaining antioxidant forms of ubiquinone and vitamin E and this enzymatic activity can be sufficient to protect against carcinogenesis. Oxidative stress may contribute to carcinogenesis as an important risk factor. This study aimed to investigate the relationship between prostate cancer and NQO1 C609T polymorphism in a Turkish population.


METHODS
Forty-five patients with prostate cancer and fifty healthy controls were included in this study. Gene polymorphism was determined by a restricted fragment length polymorphism-polymerase chain reaction (PCR-RFLP) method.


RESULTS
The NQO1 TT genotype was demonstrated to be increased in patients, there were no significant differences between distributions of NQO1 C609T genotypes in the study groups. Serum PSA and alkaline phosphatase levels were elevated in patients carrying T alleles (TT > CT > CC). There were no correlations between tumor size, node classification, metastasis or stage and NQO1 genotypes.


CONCLUSIONS
The results of our study of Turkish prostate cancer patients suggests that mutation of the NQO1 gene may effect the serum PSA and alkaline phosphatase levels. However there were no differences between the NQO1 genotypes in the study groups.
Ergen
H Arzu
HA
Institute of Experimental Medicine, Department of Molecular Medicine, Istanbul University, Capa-Istanbul, Turkey.
Gormus
Uzay
U
Narter
Fehmi
F
Zeybek
Umit
U
Bulgurcuoglu
Sibel
S
Isbir
Turgay
T
D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D000483:Alleles; D015992:Body Mass Index; D003404:Creatinine / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017430:Prostate-Specific Antigen / Q000097:blood; D011471:Prostatic Neoplasms / Q000097:blood / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D014421:Turkey
D003404:Creatinine; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D000469:Alkaline Phosphatase; D017430:Prostate-Specific Antigen