Extracellular ATP increases L-carnitine transport and content in C2C12 cells.
Extracellular ATP regulates cell proliferation, muscle contraction and myoblast differentiation. ATP present in the muscle interstitium can be released from contracting skeletal muscle cells. L-Carnitine is a key element in muscle cell metabolism, as it serves as a carrier for fatty acid through mitochondrial membranes, controlling oxidation and energy production. Treatment of C2C12 cells with 1 mmol/l of ATP induced a marked increase in L-carnitine uptake that was associated with an increase in L-carnitine content in these cells. These effects were found to be dependent on the density of the cultured cells and on the dose of ATP. The use of specific inhibitors of P2X and P2Y receptors abolished the effect of ATP on L-carnitine metabolism. As ATP can be released from stressed or exercising cells, it can be hypothesized that ATP acts as a messenger in the muscle. ATP will be released to recruit the next cells and increase their metabolism.
Rigault
Caroline
C
INSERM U866, Laboratoire de Biochimie Métabolique et Nutritionnelle, Faculté Gabriel, Université de Bourgogne, Dijon, France.
Bernard
Arnaud
A
Georges
Béatrice
B
Kandel
Adeline
A
Pfützner
Elisabeth
E
Le Borgne
Françoise
F
Demarquoy
Jean
J
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002331:Carnitine / Q000378:metabolism*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D051379:Mice; D032446:Myoblasts / Q000378:metabolism; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D014803:Vitamin B Complex / Q000378:metabolism*
D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D014803:Vitamin B Complex; D000255:Adenosine Triphosphate; D002331:Carnitine