title: Direct evidence for epithelial-mesenchymal transitions in breast cancer.
abstract: We developed stromal- and epithelial-specific cre-transgenic mice to directly visualize epithelial-mesenchymal transition (EMT) during cancer progression in vivo. Using three different oncogene-driven mouse mammary tumor models and cell-fate mapping strategies, we show in vivo evidence for the existence of EMT in breast cancer and show that myc can specifically elicit this process. Hierarchical cluster analysis of genome-wide loss of heterozygosity reveals that the incidence of EMT in invasive human breast carcinomas is rare, but when it occurs it is associated with the amplification of MYC. These data provide the first direct evidence for EMT in breast cancer and suggest that its development is favored by myc-initiated events.
authors.affiliation: Department of Molecular Genetics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Trimboli
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukino
authors.forename: Alain
authors.initials: A
authors.lastname: de Bruin
authors.forename: Guo
authors.initials: G
authors.lastname: Wei
authors.forename: Lei
authors.initials: L
authors.lastname: Shen
authors.forename: Stephan M
authors.initials: SM
authors.lastname: Tanner
authors.forename: Nicholas
authors.initials: N
authors.lastname: Creasap
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Rosol
authors.forename: Michael L
authors.initials: ML
authors.lastname: Robinson
authors.forename: Charis
authors.initials: C
authors.lastname: Eng
authors.forename: Michael C
authors.initials: MC
authors.lastname: Ostrowski
authors.forename: Gustavo
authors.initials: G
authors.lastname: Leone
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D016259:Genes, myc; D006801:Humans; D019656:Loss of Heterozygosity; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000473:pathology*; D008648:Mesoderm / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009361:Neoplasm Invasiveness; D011247:Pregnancy