[Origin of neointimal cells in autologous vein graft in rat model].
OBJECTIVE
To investigate the potential cell sources of neointimal cells in autologous vein graft in rat model.


METHODS
Vein graft neointimal cell origins were investigated using a model of vein-to-artery interposition modal. Slides were stained with hematoxylin and eosin, immunohistochemical staining was also performed with primary antibodies alpha-smooth actin or CD34.


RESULTS
Neointimal thickening was greater at the proximal ends (65.2 +/- 4.6) microm and, to a lesser extent, distal ends (64.7 +/- 5.3) microm, in comparison to the middle of the graft (63.5 +/- 5.6) microm. Vein-originating cells survived and make a contribution to neointimal formation within the vein graft, mostly adjacent to the lumen, suggesting an intimate association with endothelial cells, donor arterial smooth muscle cells or circulating progenitor cells.


CONCLUSIONS
Vein graft neointimal cells arise predominantly from vein-derived endothelial cells, donor arteria smooth muscle cells or circulating progenitor cells. It suggests clinical relevance of stenosis-inhibiting therapies directed at the vein graft or early system pharmacologic administration.
Deng
Yong-Zhi
YZ
Department of Cardiothoracic Surgery, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan, China. dengyongzhi@hotmail.com
Liu
Su-Jian
SJ
Ma
Li
L
Li
Hong-Fang
HF
Li
Yi-Fan
YF
Sun
Zong-Quan
ZQ
Chen
Jia-Jun
JJ
D000714:Anastomosis, Surgical; D000818:Animals; D017536:Carotid Artery, Common / Q000601:surgery; D006965:Hyperplasia; D007601:Jugular Veins / Q000473:pathology* / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000473:pathology*