title: Characterization of plant XRCC1 and its interaction with proliferating cell nuclear antigen.
abstract: In plants, there are no DNA polymerase beta (Pol beta) and DNA ligase III (Lig3) genes. Thus, the plant short-patch base excision repair (short-patch BER) pathway must differ considerably from that in mammals. We characterized the rice (Oryza Sativa L. cv. Nipponbare) homologue of the mammalian X-ray repair cross complementing 1 (XRCC1), a well-known BER protein. The plant XRCC1 lacks the N-terminal domain (NTD) which is required for Pol beta binding and is essential for mammalian cell survival. The recombinant rice XRCC1 (OsXRCC1) protein binds single-stranded DNA (ssDNA) as well as double-stranded DNA (dsDNA) and also interacts with rice proliferating cell nuclear antigen (OsPCNA) in a pull-down assay. Through immunoprecipitation, we demonstrated that OsXRCC1 forms a complex with PCNA in vivo. OsXRCC1 mRNA was expressed in all rice organs and was induced by application of bleomycin, but not of MMS, H(2)O(2) or UV-B. Bleomycin also increased the fraction of OsXRCC1 associated with chromatin. These results suggest that OsXRCC1 contributes to DNA repair pathways that differ from the mammalian BER system.
authors.affiliation: Department of Applied Biological Science, Tokyo University of Science, Chiba, 278-8510, Japan.
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Uchiyama
authors.forename: Yuko
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
mesh_terms: D002470:Cell Survival; D002843:Chromatin / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018520:Plant Shoots / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000378:metabolism; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D018809:Proliferating Cell Nuclear Antigen; D011994:Recombinant Proteins; D000076105:X-ray Repair Cross Complementing Protein 1