[Association of dendritic cell distribution with intima hyperplasia in rats with diabetes mellitus].
OBJECTIVE
To investigate the association of dendritic cell distribution in the peripheral blood, spleen and arterial wall with intimal hyperplasia in rats with diabetes mellitus.


METHODS
Diabetes mellitus was induced in rats by intraperitoneal injection of streptozotocin and high-fat feeding for 8 weeks. Peripheral blood, arterial wall and the spleen were collected from the rats to prepare cell suspensions, in which the proportions of dendritic cells and T cells were determined by flow cytometry.


RESULTS
The tunica intimal hyperplasia was more obvious in diabetic rats with or without high-fat feeding as compared with that of the control rats (P<0.05), and their dendritic cells decreased significantly in the peripheral blood (P<0.05) but increased in the arterial wall. The percentage of T cells was also increased in the peripheral blood and arterial wall of the diabetic rats.


CONCLUSIONS
Changes in the distribution of dendritic cells and T cells are closely associated with intimal hyperplasia in diabetic rats, suggesting the involvement of dendritic cells and T cells in the formation of atherosclerosis.
Fu
Qiang
Q
Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. fq-tong@sohu.com
Li
Zhi-liang
ZL
Fu
Xiao-hua
XH
Lei
Xiao
X
Miao
Fei
F
Wu
Hong-chao
HC
Liu
Ying-feng
YF
D000818:Animals; D003713:Dendritic Cells / Q000166:cytology*; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000473:pathology; D006965:Hyperplasia / Q000150:complications / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology; D013311:Streptozocin; D013601:T-Lymphocytes / Q000166:cytology; D017539:Tunica Intima / Q000473:pathology*
D013311:Streptozocin