title: Analysis of eukaryotic translation in purified and semipurified systems.
abstract: Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.
authors.affiliation: Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Merrick
authors.forename: J O
authors.initials: JO
authors.lastname: Hensold
mesh_terms: D000818:Animals; D002457:Cell Extracts; D003584:Cytological Techniques; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis / Q000502:physiology*; D012270:Ribosomes / Q000502:physiology*
chemical_list: D002457:Cell Extracts