title: Determination of icariin in rat plasma by reverse-phase high-performance liquid chromatography after oral administration of a lipid-based suspension of Epimedium koreanum extract.
abstract: A simple, specific and sensitive method for quantitative determination of icariin in rat plasma using reverse-phase high-performance liquid chromatography with UV-detection was developed and applied to an animal study of a lipid-based suspension of the Epimedium koreanum extract in rats. Rutin was selected as the internal standard and methanol was found to be the best solvent for extraction of icariin from the plasma. Linearity was observed between 0.030 and 100 microg/mL (r > 0.99). The extraction recoveries of icariin and rutin were approximately 75 and 80%, respectively, in plasma. The intra- and inter-day coefficients of variation were less than 5%. The limit of detection was 6 ng/mL and the limit of quantification was 18 ng/mL.
authors.affiliation: Interregional Center 'Adaptogen', 47/5, Piskarevsky pr., 195067, St Petersburg, Russia.
authors.forename: Olga N
authors.initials: ON
authors.lastname: Pozharitskaya
authors.forename: Marina V
authors.initials: MV
authors.lastname: Karlina
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Shikov
authors.forename: Vera M
authors.initials: VM
authors.lastname: Kosman
authors.forename: Marina N
authors.initials: MN
authors.lastname: Makarova
authors.forename: Valery G
authors.initials: VG
authors.lastname: Makarov
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000097:blood*; D008055:Lipids / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005419:Flavonoids; D008055:Lipids; D010936:Plant Extracts; C056599:icariin