title: Effect of meat cooking on physicochemical state and in vitro digestibility of myofibrillar proteins.
abstract: The effect of meat cooking was measured on myofibrillar proteins from bovine M. Rectus abdominis. The heating treatment involved two temperatures (100 degrees C during 5, 15, 30, and 45 min and 270 degrees C during 1 min). Protein oxidation induced by cooking was evaluated by the level of carbonyl and free thiol groups. Structural modifications of proteins were assessed by the measurement of their surface hydrophobicity and by their aggregation state. With the aim of evaluating the impact of heat treatment on the digestive process, myofibrillar proteins were then exposed to proteases of the digestive tract (pepsin, trypsin, and alpha-chymotrypsin) in conditions of pH and temperature that simulate stomach and duodenal digestion. Meat cooking affected myofibrillar protein susceptibility to proteases, with increased or decreased rates, depending on the nature of the protease and the time/temperature parameters. Results showed a direct and quantitative relationship between protein carbonylation (p<0.01) and aggregation (p<0.05) induced by cooking and proteolytic susceptibility to pepsin. However, no such correlations have been observed with trypsin and alpha-chymotrypsin.
authors.affiliation: INRA, UR370 QuaPA, F-63122 Saint-Genès Champanelle, France.
authors.forename: Veronique
authors.initials: V
authors.lastname: Santé-Lhoutellier
authors.forename: Thierry
authors.initials: T
authors.lastname: Astruc
authors.forename: Penka
authors.initials: P
authors.lastname: Marinova
authors.forename: Eleonore
authors.initials: E
authors.lastname: Greve
authors.forename: Philippe
authors.initials: P
authors.lastname: Gatellier
mesh_terms: D000818:Animals; D002417:Cattle; D003296:Cooking; D004063:Digestion; D005511:Food Handling / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000032:analysis*; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry*; D009210:Myofibrils; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000378:metabolism; D013696:Temperature
chemical_list: D009124:Muscle Proteins; D010447:Peptide Hydrolases