Phenotypic and functional analysis of murine resident and induced peritoneal macrophages.
Primary macrophages from the peritoneal cavities of mice are commonly used ex vivo to produce inflammatory cytokines and test anti-inflammatory agents. Although approximately 1 million peritoneal macrophages can be obtained from an untreated mouse, more than twice that number can be collected 48 to 72 h after intraperitoneal injection of sterile inducing agents such as Brewer thioglycollate broth, casein, and proteose peptone. However, whether 'induced' macrophages are functionally equivalent to 'resident' peritoneal macrophages has been unclear. Flow cytometric analysis revealed significant phenotypic differences between these 2 macrophage types. Resident and induced peritoneal macrophages also demonstrated markedly different capacities to produce the inflammatory cytokines interleukins 6 and 1beta in response to lipopolysaccharide stimulation in vitro. Increased understanding of the differences between resident and induced peritoneal macrophages likely will help investigators decide which macrophage type is appropriate for their in vitro assay needs.
Turchyn
Lyubomyr R
LR
Department of Comparative Medicine, Pfizer Global Research and Development, Ann Arbor, MI, USA. compdvm@yahoo.com
Baginski
Theodore J
TJ
Renkiewicz
Richard R
RR
Lesch
Charles A
CA
Mobley
James L
JL
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D018189:Immunomagnetic Separation; D066298:In Vitro Techniques; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000145:classification* / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D019463:Mice, Inbred MRL lpr; D016688:Mice, Inbred NOD; D008815:Mice, Inbred Strains; D010641:Phenotype; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015054:Zymosan / Q000494:pharmacology
D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015054:Zymosan