Diagnostic role of biliary pancreatic elastase for cholangiocarcinoma in patients with cholestasis.
BACKGROUND
A wide array of proteins is secreted into the bile and may be associated with biliary tract diseases. We attempted to discover novel biomarker in bile for cholangiocarcinoma.


METHODS
Bile was collected from patients with bile duct obstruction. Proteins were separated by 2-dimensional electrophoresis and identified by mass spectrometry. Levels of mRNA and protein expression of the candidate biomarker were analyzed by real-time PCR and Western blotting, respectively, whereas enzyme activity was measured by a kinetic method. The diagnostic efficacy was assessed by receiver operating characteristic (ROC) curve analysis.


RESULTS
Pancreatic elastase (PE) 3B was identified as a biomarker for cholangiocarcinoma. The mRNA of PE 3B was up-regulated in cancerous tissues, compared to non-cancerous tissues. The protein expression and enzyme activity of PE in bile were increased in patients with cholangiocarcinoma, compared to gallstone patients. Biliary amylase activity was used to correct the presence of pancreaticobiliary reflux. Significantly higher PE/amylase ratios in bile were found in patients with cholangiocarcinoma (0.214+/-0.045) than those with gallstone (0.023+/-0.005, p<0.001). The area under the ROC curve of the ratio was 0.877 (95% CI: 0.765 to 0.988). Using 0.065 as a cutoff value, the ratio distinguished malignant from benign causes of biliary obstruction with a sensitivity of 82% and a specificity of 89%.


CONCLUSIONS
PE in bile is a biomarker for cholangiocarcinoma and the combination measurement of PE and amylase enhances diagnostic efficacy.
Chen
Chiung-Yu
CY
Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Tsai
Wei-Lun
WL
Wu
Hui-Chun
HC
Syu
Mei-Jywan
MJ
Wu
Cheng-Chung
CC
Shiesh
Shu-Chu
SC
D001483:Base Sequence; D001659:Biliary Tract / Q000201:enzymology*; D018281:Cholangiocarcinoma / Q000150:complications / Q000175:diagnosis*; D002779:Cholestasis / Q000150:complications*; D017931:DNA Primers; D006801:Humans; D010196:Pancreatic Elastase / Q000378:metabolism*
D017931:DNA Primers; D010196:Pancreatic Elastase