Production of biological active murine IFN-gamma by recombinant Lactococcus lactis.
IFN-gamma is a cytokine produced primarily by both T lymphocytes and natural killer cells and it is considered to be an attractive therapeutic molecule. In the present study, a DNA sequence encoding the mature murine IFN-gamma (muIFN-gamma) protein was cloned and expressed in the food-grade lactic acid bacterium Lactococcus lactis. The activity of recombinant muIFN-gamma produced by genetically engineered L. lactis was confirmed in an antiviral assay using MoV cells infected with Vesicular Stomatitis Virus. The data provide the first demonstration that a Gram-positive bacterium, L. lactis, is able to produce functional muIFN-gamma. This recombinant strain could lead to the development of a new, well-tolerated vector to deliver active muIFN-gamma at the mucosal level.
Bermúdez-Humarán
Luis G
LG
Unité d'Ecologie et de Physiologie du Système Digestif, Domaine de Vilvert, Jouy-en-Josas, France. luis.bermudez@jouy.inra.fr
Cortes-Perez
Naima G
NG
L'Haridon
René
R
Langella
Philippe
P
D000818:Animals; D002460:Cell Line; D005822:Genetic Vectors / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D011994:Recombinant Proteins; D007371:Interferon-gamma