title: Cyclodextrin production and genetic characterization of cyclodextrin glucanotranferase of Paenibacillus graminis.
abstract: Paenibacillus graminis strains were described recently as cyclodextrin (CD) producers. Cyclodextrins are produced by cyclodextrin glucanotransferase (CGTase) which has not been characterized in P. graminis. Similar amounts of alpha- and beta-CDs were produced by P. graminis (MC22.13) and P. macerans (LMD24.10(T)). Primers were designed to sequence the gene encoding CGTase from P. graminis. A phylogenetic tree was constructed and P. graminis CGTase protein showed to be closer (79.4% protein identity) to P. macerans |P31835|. Hybridization studies suggested that the gene encoding CGTase is located in different positions in the genomes of P. macerans and P. graminis.
authors.affiliation: Laboratório de Genética Microbiana, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brasil.
authors.forename: Renata Estebanez
authors.initials: RE
authors.lastname: Vollú
authors.forename: Fabio Faria
authors.initials: FF
authors.lastname: da Mota
authors.forename: Eliane Aparecida
authors.initials: EA
authors.lastname: Gomes
authors.forename: Lucy
authors.initials: L
authors.lastname: Seldin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003505:Cyclodextrins / Q000096:biosynthesis*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics*; D016937:Gram-Positive Endospore-Forming Rods / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D003505:Cyclodextrins; D017931:DNA Primers; D004269:DNA, Bacterial; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase