Two-stage glycerol feeding for enhancement of recombinant hG-CSF production in a fed-batch culture of Pichia pastoris.
Recombinant hG-CSF was expressed in Pichia pastoris under the control of the AOX1 promoter. In this study, the glycerol feeding rate was adjusted to achieve the maximum attainable specific growth rate before induction. Using a two-stage glycerol feeding method, the specific growth rate was changed from a maximum value of 0.21 h(-1) (at the beginning of feeding) to 0.15 h(-1) prior to induction. With this approach, the final dry cell wt and rhG-CSF yield achieved was close to 120 g l(-1) and 320 mg l(-1), respectively. Our study found that the two-stage feeding method allowed the overall productivity of rhG-CSF to increase 2.9 times that of the conventional fed-batch method.
Bahrami
A
A
Biotechnology Group, Department of Chemical Engineering, Faculty of Engineering, Tarbiat Modares University, Tehran, P.O. Box 14155-143, I.R. Iran.
Shojaosadati
S A
SA
Khalilzadeh
R
R
Farahani
E Vasheghani
EV
D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D010843:Pichia / Q000378:metabolism*; D011994:Recombinant Proteins
D003470:Culture Media; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D005990:Glycerol