title: Synthesis of fluorous tags for incorporation of reducing sugars into a quantitative microarray platform.
abstract: Carbohydrate microarrays can map out key interactions of carbohydrates and proteins in a high-throughput manner, but require the inclusion of a range of sugars for their optimal use. Here we present the synthesis and use of a new hydroxylamine-modified fluorous tag that allows the facile incorporation of reducing sugars into a noncovalent fluorous-based microarray after simple purification by fluorous solid-phase extraction (FSPE). The microarray supports quantitative screening against carbohydrate-binding proteins.
authors.affiliation: Department of Chemistry and the Plant Sciences Institute, Gilman Hall, Iowa State University, Ames, Iowa 50011-3111, USA.
authors.forename: Guo-Song
authors.initials: GS
authors.lastname: Chen
authors.forename: Nicola L
authors.initials: NL
authors.lastname: Pohl
mesh_terms: D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry*; D006031:Glycosylation; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis* / Q000737:chemistry; D019811:Hydroxylamine / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D013237:Stereoisomerism; D013499:Surface Properties
chemical_list: D002241:Carbohydrates; D006845:Hydrocarbons, Fluorinated; D011506:Proteins; D019811:Hydroxylamine