title: Two-photon excitation microscopy for the study of living cells and tissues.
abstract: Two-photon excitation microscopy is an alternative to confocal microscopy that provides advantages in three-dimensional and deep tissue imaging. This unit will describe the basic physical principles of two-photon excitation and discuss the advantages and limitations of its use in laser-scanning microscopy. The advantages of two-photon microscopy are reduced phototoxicity, increased imaging depth, and the ability to initiate localized photochemistry.
authors.affiliation: Vanderbilt University, Nashville, Tennessee, USA.
authors.forename: Jonathan V
authors.initials: JV
authors.lastname: Rocheleau
authors.forename: David W
authors.initials: DW
authors.lastname: Piston
mesh_terms: D000818:Animals; D002470:Cell Survival; D008853:Microscopy / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D009249:NADP / Q000378:metabolism; D038761:Photobleaching; D017785:Photons; D012754:Sharks
chemical_list: D009249:NADP