title: Strategies for systems-level metabolic engineering.
abstract: Bio-based production of chemicals, fuels and materials is becoming more and more important due to the increasing environmental problems and sharply increasing oil price. To make these biobased processes economically competitive, the biotechnology industry explores new ways to improve the performance of microbial strains in fermentation processes. In contrast to the random mutagenesis and/or intuitive local metabolic engineering practiced in the past, we are now moving towards global-scale metabolic engineering, aided by various experimental and computational tools. This has recently led to some remarkable achievements for the overproduction of valueadded products. In this review, we highlight several relevant gene manipulation tools and computational tools using genome-scale stoichiometric models, and provide useful strategies for successful metabolic engineering along with selected exemplary studies.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Kim
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Sohn
authors.forename: Hyun Uk
authors.initials: HU
authors.lastname: Kim
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D024861:Genetic Enhancement / Q000379:methods*; D015202:Protein Engineering / Q000639:trends*; D020543:Proteome / Q000378:metabolism*; D049490:Systems Biology / Q000379:methods* / Q000639:trends*
chemical_list: D020543:Proteome