Serodiagnosis of recently acquired Toxoplasma gondii infection in pregnant women using enzyme-linked immunosorbent assays with a recombinant dense granule GRA6 protein.
Indirect immunoglobulin G (IgG) and IgM enzyme-linked immunosorbent assays (ELISAs) with a recombinant GRA6 protein of Toxoplasma gondii were developed and evaluated for accurate diagnosis of recently acquired infection in pregnant women. According to the results from Toxoplasma serodiagnostic tests, women were classified into 3 groups representing acute (group I), chronic (group II), or no Toxoplasma infection (group III). To discriminate group I from group II sera, the GRA6-IgG-ELISA reached sensitivity and specificity of 87.5% and 94.1%, respectively. Although 22 (91.7%) of 24 group I sera were positive by the GRA6-IgM-ELISA, only 1 (2.9%) of 34 group II sera scored positive. The GRA6-IgM-ELISA displayed a meaningful correlation with Vidas Toxo IgM and exhibited higher specificity (97.1%) than Euroimmun IgM ELISA (88.2%) (Euroimmun, Lübeck, Germany) for detection of recent infection. These results demonstrate that IgG and IgM ELISA with rGRA6 are useful to identify and discriminate recent from past Toxoplasma infection in pregnant women.
Golkar
Majid
M
Molecular Parasitology Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran 1316943551, Iran. golkar@pasteur.ac.ir
Azadmanesh
Kayhan
K
Khalili
Ghader
G
Khoshkholgh-Sima
Baharak
B
Babaie
Jalal
J
Mercier
Corinne
C
Brenier-Pinchart
Marie-Pierre
MP
Fricker-Hidalgo
Hélène
H
Pelloux
Hervé
H
Cesbron-Delauw
Marie-France
MF
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000175:diagnosis*; D037841:Pregnant People; D015800:Protozoan Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C095047:GRA6 protein, Toxoplasma gondii; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015800:Protozoan Proteins