title: An up-to-date anti-cancer treatment strategy focusing on HIF-1alpha suppression: its application for refractory ovarian cancer.
abstract: Hypoxia inducible factor-1alpha (HIF-1alpha) predominantly determines the transcriptional activity of HIF-1, which induces the certain genetic expressions to participate in the proliferation and progression of the tumor. It is supposed that HIF-1alpha is also an extremely important factor in cancer treatment. Based on the results of our recent analyses using ovarian tumors, which indicated the close association of HIF-1alpha expression with the acquisition of malignancy and the characterization of histology, we further investigated the possibility of a new strategy of cancer therapy that targeted HIF-1alpha inhibition in the ovarian carcinoma. The cell line HUOCA-II, which originates from the refractory ovarian clear cell adenocarcinoma, was treated with rapamycin. The inhibitory effect of HIF-1alpha was analyzed by immunohistochemistry and western blotting. It was demonstrated that inhibition of HIF-1alpha and vascular endothelial growth factor (VEGF) expressions would lead to the down-regulation of tumor cell proliferation. Interestingly, there was little or no change in GLUT-1 expression by rapamycin administration. Thus, the inhibition of GLUT-1 may also be a key for the new strategy of cancer therapy as well as HIF-1alpha and VEGF.
authors.affiliation: Department of Pathology, Tokai University School of Medicine, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Fujita
authors.forename: Masanori
authors.initials: M
authors.lastname: Yasuda
authors.forename: Kanae
authors.initials: K
authors.lastname: Kitatani
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Hirabayashi
authors.forename: Susumu
authors.initials: S
authors.lastname: Takekoshi
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Iida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hirasawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.forename: Mikio
authors.initials: M
authors.lastname: Mikami
authors.forename: Isamu
authors.initials: I
authors.lastname: Ishiwata
authors.forename: Michio
authors.initials: M
authors.lastname: Shimizu
authors.forename: R Yoshiyuki
authors.initials: RY
authors.lastname: Osamura