title: A cloned toy poodle produced from somatic cells derived from an aged female dog.
abstract: To date, dogs have been cloned with somatic cell nuclear transfer (SCNT), using donor cells derived from large-breed dogs 2 months to 3 years of age. The objective of the present study was to use SCNT to produce a small-breed dog from ear fibroblasts of an aged poodle, using large-breed oocyte donors and surrogate females, and to determine the origin of its mitochondrial DNA (mtDNA) and the length of its telomeres. Oocytes were derived from large-breed donors, matured in vivo, collected by flushing oviducts, and reconstructed with somatic cells derived from an aged (14-year-old) female toy poodle. Oocytes and donor cells were fused by electric stimuli, activated chemically, and transferred into the oviducts of large-breed recipient females. Overall, 358 activated couplets were surgically transferred into the oviducts of 20 recipient dogs. Two recipients became pregnant; only one maintained pregnancy to term, and a live puppy (weighing 190 g) was delivered by Caesarean section. The cloned poodle was phenotypically and genetically identical to the nuclear donor dog; however, its mtDNA was from the oocyte donor, and its mean telomere length was not significantly different from that of the nuclear donor. In summary, we demonstrated that a small-breed dog could be cloned by transferring activated couplets produced by fusion of somatic cells from a small-breed, aged donor female with enucleated in-vivo-matured oocytes of large-breed females, and transferred into the oviduct of large-breed recipient female dogs.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: G
authors.initials: G
authors.lastname: Jang
authors.forename: S G
authors.initials: SG
authors.lastname: Hong
authors.forename: H J
authors.initials: HJ
authors.lastname: Oh
authors.forename: M K
authors.initials: MK
authors.lastname: Kim
authors.forename: J E
authors.initials: JE
authors.lastname: Park
authors.forename: H J
authors.initials: HJ
authors.lastname: Kim
authors.forename: D Y
authors.initials: DY
authors.lastname: Kim
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002585:Cesarean Section / Q000662:veterinary; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D004285:Dogs / Q000235:genetics / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D018895:Microsatellite Repeats / Q000235:genetics; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009865:Oocytes / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics / Q000502:physiology
chemical_list: D004272:DNA, Mitochondrial