[Protective effect of NEP on Abeta-induced apoptosis in PC12 cells].
OBJECTIVE
To identify the effect of NEP on Abeta -induced apoptosis in PC12 cells.


METHODS
PC12 cells that stably express NEP is generated and the effect of NEP on the process of apoptosis induced by Abeta is analyzed, including the viability of the cells, the production of LDH, ROS and ATP, the activity of Caspase-3.


RESULTS
NEP could improve the viability of cells and the production of ATP, inhibit the release of LDH and ROS. In the same time, the activity of caspase-3 descended (P < 0.05). But iNEP had not significant effect on cells apoptosis (P > 0.05).


CONCLUSIONS
NEP has the protective effect on Abeta-induced apoptosis in PC12 cells.
Zeng
Ji-Ping
JP
Biochemistry and Molecular Biology Department, School of Medicine, Shandong University, Jinan, 250012, China.
Yang
Ling-Ling
LL
Wu
Wei-Fang
WF
Kong
Feng
F
Hu
Xiao-Yan
XY
Cui
Xing
X
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D014162:Transfection
D016229:Amyloid beta-Peptides; D010450:Endopeptidases; C505410:Casp3 protein, rat; D053148:Caspase 3