title: A cell-repellent sulfonated PEG comb-like polymer for highly resolved cell micropatterns.
abstract: This paper investigates the chemical modification of a cell-repellent poly(ethylene glycol) (PEG)-based polymer to enhance its hydrophilicity with sulfonate groups, and its application in the fabrication of a cell microarray. First, a polymer comprised of a methyl methacrylate (MMA) backbone with PEG side-chains (PMMA-b-PEG) was synthesized from three monomers by radical polymerization and purified. Despite the hydrophilic side-groups in the amphiphilic polymer, the backbone structure's hydrophobicity allows for local adsorption of biomolecules in incubation media with or without serum. To enhance the hydrophilicity of the polymer, we tethered sulfonate groups to the hydroxyl groups on the PEG side chains (PMMA-b-PEG-SO3). The sulfate groups' physical and mechanical movement competitively repels biomolecules approaching the PMMA-b-PEG surface. Polymers modified with sulfonate were characterized by contact angle measurement, FT-IR, NMR, AFM and GPC. PMMA-b-PEG and PMMA-b-PEG-SO3 were successfully micropatterned on polystyrene and glass surfaces, and cell attachment was performed in either serum-free or serum-containing media, resulting in highly resolved cell micropatterns.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-742, South Korea.
authors.forename: Jaeyeon
authors.initials: J
authors.lastname: Jung
authors.forename: Kyunga
authors.initials: K
authors.lastname: Na
authors.forename: Byungcheol
authors.initials: B
authors.lastname: Shin
authors.forename: Okgene
authors.initials: O
authors.lastname: Kim
authors.forename: Jonghwan
authors.initials: J
authors.lastname: Lee
authors.forename: Kyusik
authors.initials: K
authors.lastname: Yun
authors.forename: Jinho
authors.initials: J
authors.lastname: Hyun
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D001704:Biopolymers; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry*; D013461:Sulfur Oxides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D013450:Sulfones; D013461:Sulfur Oxides; D011092:Polyethylene Glycols; D019904:Polymethyl Methacrylate