title: Radiographic and histological evaluation of bisphosphonate alendronate and metotrexate effects on rat mandibles inoculated with Walker 256 carcinosarcoma.
abstract: OBJECTIVE
  To investigate the effects of bisphosphosnate alendronate (ALD) and metotrexate (MTX) on an experimental model of Walker 256 carcinosarcoma developed in the oral cavity of rats. METHODS Walker 256 carcinosarcoma cell suspension (0,1 mL) containing 10(6) cell/mL was implanted in the alveoli of the first and second molars. The animals were divided and treated with saline, MTX, ALD, and MTX plus ALD. Later, the animals were sacrificed, the tumors were measured and the mandibles were removed for radiographic and histological analysis.
  
  
  RESULTS
  In the control group, the radiographic images demonstrated radioluscency with poorly defined borders, and the microscopic examination revealed tumor infiltration into the peripheral and central regions of the bone. Areas of necrosis were commonly seen. In the treated groups with ALD, associated or not with MTX, the radiographic analysis revealed circumscribed tumor-induced osteolysis and various degrees of radiotransparence; while, histologically, preserved bone trabeculae with osteoid formation was observed among malignant cells.
  
  
  CONCLUSIONS
  The bisphosphonate alendronate exherted an osteoprotective effect and induced bone neoformation on the Walker 256 carcinosarcoma inoculated in rat mandibles. The combination of metotrexate with bisphosphonate alendronate is more successful than treatment with the agents alone in controlling the growth of neoplastic cells and in stimulating reactive new bone. Therefore, this may be an alternative treatment to malignant lesions of maxillaries with osteolysis.
authors.affiliation: Department of Oral Pathology, UFC, Brazil. ananune@terra.com.br
authors.forename: Ana Paula Negreiros Nunes
authors.initials: AP
authors.lastname: Alves
authors.forename: Cláudia do O
authors.initials: Cdo O
authors.lastname: Pessoa
authors.forename: Letícia
authors.initials: L
authors.lastname: Costa-Lotufo
authors.forename: Manoel Odorico
authors.initials: MO
authors.lastname: Moraes Filho
mesh_terms: D019386:Alendronate / Q000627:therapeutic use*; D000818:Animals; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D002279:Carcinoma 256, Walker / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000473:pathology; D004359:Drug Therapy, Combination; D005260:Female; D008339:Mandibular Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000473:pathology; D008727:Methotrexate / Q000627:therapeutic use*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050071:Bone Density Conservation Agents; D019386:Alendronate; D008727:Methotrexate