Cell wound assays.
Many mammalian and invertebrate cells that reside in mechanically active tissue environments normally and frequently experience disruptions in plasma membrane integrity which are rapidly repaired and do not lead to cellular death. This unit describes methods for identifying "wounded" cells, often present as a minority in culture or tissues. The use of these methods can provide information about the frequency and extent of cellular wounding.
McNeil
P L
PL
Medical College of Georgia, Augusta, Georgia, USA.
Clarke
M F
MF
Miyake
K
K
D000418:Albumins; D000818:Animals; D001681:Biological Assay / Q000379:methods* / Q000592:standards; D002462:Cell Membrane / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D051381:Rats; D012038:Regeneration / Q000502:physiology*; D046509:Tissue Culture Techniques; D014945:Wound Healing / Q000502:physiology*
D000418:Albumins; D005456:Fluorescent Dyes