Determination of active ingredients in corn silk, leaf, and kernel by capillary electrophoresis with electrochemicaI detection.
Corn has been known for its accumulation of flavones and phenolic acids. However, many parts of corn, except kernel, have not drawn much attention. In this work, a method based on capillary zone electrophoresis with electrochemical detection has been used for the separation and determination of epicatechin, rutin, ascorbic acid (Vc), kaempferol, chlorogenic acid, and quercetin in corn silk, leaf, and kernel. The distribution comparison of the ingredients among silk, leaf, and kernel is discussed. Several important factors--including running buffer acidity, separation voltage, and working electrode potential--were evaluated to acquire the optimum analysis conditions. Under the optimum conditions, the analytes could be well separated within 19 min in a 40-mmol/L borate buffer (pH 9.2). The response was linear over three orders of magnitude with detection limits (S/N = 3) ranging from 4.97 x 10(-8) to 9.75 x 10(-8) g/mL. The method has been successfully applied for the analysis of corn silk, leaf, and kernel with satisfactory results.
Lin
Miao
M
Department of Chemistry, East China Normal University, Shanghai, PR China. linmiaoO224@hotmail.com
Chu
Qing-Cui
QC
Tian
Xiu-Hui
XH
Ye
Jian-Nong
JN
D001205:Ascorbic Acid / Q000032:analysis; D002021:Buffers; D002392:Catechin / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis; D004365:Drugs, Chinese Herbal; D004566:Electrodes; D019075:Electrophoresis, Capillary; D035264:Flowers / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D044949:Kaempferols / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis; D012680:Sensitivity and Specificity; D055672:Static Electricity; D013997:Time Factors; D003313:Zea mays / Q000737:chemistry*
D002021:Buffers; D004365:Drugs, Chinese Herbal; D044949:Kaempferols; D002726:Chlorogenic Acid; D012431:Rutin; C006552:kaempferol; D002392:Catechin; D011794:Quercetin; D001205:Ascorbic Acid