Production of polygalacturonase from Coriolus versicolor grown on tomato pomace and its chromatographic behaviour on immobilized metal chelates.
Tomato pomace and pectin were used as the sole carbon sources for the production of polygalacturonase from a strain of Coriolus versicolor in submerged culture. The culture of C. versicolor grown on tomato pomace exhibited a peak of polygalacturonase activity (1,427 U/l) on the third day of culture with a specific activity of 14.5 U/mg protein. The production of polygalacturonase by C. versicolor grown on pectin as a sole carbon source increased with the time of cultivation, reaching a maximum activity of 3,207 U/l of fermentation broth with a specific activity of 248 U/mg protein. The levels of different isoenzymes of polygalacturonase produced during the culture growth were analysed by native PAGE. Differential chromatographic behaviour of lignocellulosic enzymes produced by C. versicolor (i.e. polygalacturonase, xylanase and laccase) was studied on immobilized metal chelates. The effect of ligand concentration, pH, the length of spacer arm and the nature of metal ion were studied for enzyme adsorption on immobilized metal affinity chromatography (IMAC). The adsorption of these lignocellulosic enzymes onto immobilized metal chelates was pH-dependent since an increase in protein adsorption was observed as the pH was increased from 6.0 to 8.0. The adsorption of polygalacturonase as well as other enzymes to immobilized metal chelates was due to coordination of histidine residues which are available at the protein surface since the presence of imidazole in the equilibration buffer abolished the adsorption of the enzyme to immobilized metal chelates. A one-step purification of polygalacturonase from C. versicolor was devised by using a column of Sepharose 6B-EPI 30-IDA-Cu(II) and purified enzyme exhibited a specific activity of about 150 U/mg protein, final recovery of enzyme activity of 100% and a purification factor of about 10. The use of short spacer arm and the presence of imidazole in equilibration buffer exhibited a higher selectivity for purification of polygalacturonase on this column with a high purification factor. The purified enzyme preparation was analysed by SDS-PAGE as well as by "in situ" detection of enzyme activity.
do Rosário Freixo
Maria
M
Department of Chemistry, Universidade de Evora, Rua Ramalho Ortigão N 59, Evora, Portugal.
Karmali
Amin
A
Arteiro
José Maria
JM
D002241:Carbohydrates / Q000032:analysis; D002614:Chelating Agents / Q000737:chemistry*; D002847:Chromatography, Agarose; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D018551:Solanum lycopersicum / Q000378:metabolism*; D008670:Metals / Q000737:chemistry*; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000737:chemistry; D011124:Polyporaceae / Q000201:enzymology*; D011506:Proteins / Q000032:analysis
D002241:Carbohydrates; D002614:Chelating Agents; D003470:Culture Media; D008670:Metals; D011506:Proteins; D011096:Polygalacturonase