title: Effects of bioactive glass and beta-TCP containing three-dimensional laser sintered polyetheretherketone composites on osteoblasts in vitro.
abstract: Because of their excellent physical properties nonresorbable thermoplastic polymers have become more important for the field of reconstructive surgery. Aim of the present study was to investigate the effects of laser sintered polyetheretherketone (PEEK) with incorporated osteoconductive and bioactive bone substitution materials on osteoblasts in vitro. Human osteoblasts (hFOB 1.19) were seeded onto laser sintered PEEK samples containing nano-sized carbon black, beta-tricalciumphosphate (beta-TCP), and bioactive glass 45S5. Osteoblasts were investigated for cell viability, cell proliferation and cell morphology. A constant proliferation of osteoblasts could be observed on all samples with the highest values for bioactive glass containing samples at day 7 (OD 1.76 +/- 0.22) and day 14 (OD 3.75 +/- 0.31) and lowest values for beta-TCP containing probes throughout the study compared with the PEEK pure control group. Highest cell viability was observed for Bioglass containing probes (95.5 +/- 3.32)% whereas osteoblasts seeded on beta-TCP containing probes showed reduced viability (84.4 +/- 4.32)%. Laser sintered PEEK implants seem to be attractive candidates for use as bone substitutes for reconstructive surgery because of their biocompatibility, individual shape, and the possibility of compounding bioinert polymer powder with osteoconductive and bioactive materials which might benefit bone formation in vivo.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Friedrich-Alexander-University, Erlangen-Nuremberg, Erlangen, Germany. cornelius.von-wilmowsky@uk-erlangen.de
authors.forename: Cornelius
authors.initials: C
authors.lastname: von Wilmowsky
authors.forename: Eleftherios
authors.initials: E
authors.lastname: Vairaktaris
authors.forename: Dirk
authors.initials: D
authors.lastname: Pohle
authors.forename: Thomas
authors.initials: T
authors.lastname: Rechtenwald
authors.forename: Rainer
authors.initials: R
authors.lastname: Lutz
authors.forename: Helmut
authors.initials: H
authors.lastname: Münstedt
authors.forename: Garrit
authors.initials: G
authors.lastname: Koller
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
authors.forename: Friedrich Wilhelm
authors.initials: FW
authors.lastname: Neukam
authors.forename: Karl Andreas
authors.initials: KA
authors.lastname: Schlegel
authors.forename: Emeka
authors.initials: E
authors.lastname: Nkenke
mesh_terms: D001577:Benzophenones; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D002516:Ceramics; D005898:Glass / Q000737:chemistry*; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000494:pharmacology; D007834:Lasers; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers
chemical_list: D001577:Benzophenones; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D007659:Ketones; D011108:Polymers; C485817:beta-tricalcium phosphate; C425330:bioactive glass 45S5; C063834:polyetheretherketone; D011092:Polyethylene Glycols