miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation.
Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.
Mizuno
Yosuke
Y
Division of Functional Genomics & Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka City, Saitama 350-1241, Japan.
Yagi
Ken
K
Tokuzawa
Yoshimi
Y
Kanesaki-Yatsuka
Yukiko
Y
Suda
Tatsuo
T
Katagiri
Takenobu
T
Fukuda
Toru
T
Maruyama
Masayoshi
M
Okuda
Akihiko
A
Amemiya
Tomoyuki
T
Kondoh
Yasumitsu
Y
Tashiro
Hideo
H
Okazaki
Yasushi
Y
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D015536:Down-Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*
D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse