Association between serum soluble membrane type matrix metalloproteinase-1 (MT1-MMP) levels and bone mineral density, and biochemical markers in postmenopausal women.
BACKGROUND
Recently, membrane type matrix metalloproteinase-1 (MT1-MMP) was found to participate in bone metabolism. We investigated the relationship between serum MT1-MMP and bone mineral density (BMD) as well as bone metabolic markers in 206 Chinese postmenopausal women aged 43-80 years.


METHODS
Western analysis and ELISA were performed to detect serum soluble MT1-MMP levels. BMD was measured by dual energy X-ray absorptiometry (DXA). Serum alkaline phosphatase (BAP) and N-telopeptides of type I collagen (NTX) were assayed using ELISA.


RESULTS
We found that soluble MT1-MMP abundantly existed in human serum as protein lack of transmembrane domain. Serum MT1-MMP levels were detectable in all participants and the range of value was 221.2-863.0 ng/ml (435.6+/-98.2 ng/ml). We found a significant negative weaker correlation between MT1-MMP and BMD at lumbar spine, total hip (Thip), and femoral neck (FN) (all P<0.05). After adjustment for age and BMI, the correlation with BMD at FN and Thip disappeared (all P>0.05). Multiple linear stepwise regression analysis showed that MT1-MMP was not a determinant factor for BMD. The significant positive correlations between MT1-MMP and BAP, NTX were found, and remained significant after adjustment for age and BMI (all P<0.05). Moreover, serum MT1-MMP, BAP, and NTX decreased in response to alendronate therapy.


CONCLUSIONS
Circulating MT1-MMP and bone turnover markers are correlated, and serum MT1-MMP levels may rise with increase in bone turnover.
Ren
Xiao-Hong
XH
Nursing College of Central South University, 168# Tong-Zi-Po Road, Changsha, Hunan 410013, PR China.
Peng
Xiao-Ding
XD
Wu
Xian-Ping
XP
Liao
Er-Yuan
EY
Sun
Zhen-Qiu
ZQ
D015502:Absorptiometry, Photon; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D015153:Blotting, Western; D015519:Bone Density; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000097:blood*; D008875:Middle Aged; D017698:Postmenopause; D012995:Solubility
D015415:Biomarkers; D053511:Matrix Metalloproteinase 14