Development of a five ChX STRs loci typing system.
We investigated the polymorphism of five X-chromosomal short tandem repeat markers (ChrX STRs) loci (DXS7132, DXS981, DXS6803, DXS6809, and DXS6789) and their value for forensic applications. A fluorescent multiplex polymerase chain reaction (PCR) for amplifying five ChrX STRs loci in the same PCR reaction was set up. A total of 827 unrelated individuals of the Han nationality in China were tested. The results show that the five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing, particularly for difficult paternity-deficient cases.
Liu
Qiu-ling
QL
Faculty of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, No.74 ZhongShan Road II, Guangzhou 510089, China. liuql@mail.sysu.edu.cn
Lv
De-jian
DJ
Wu
Xiang-lin
XL
Sun
Hong-yu
HY
Wu
Xin-yao
XY
Lu
Hui-ling
HL
D002681:China; D041321:Chromosomes, Human, X; D016172:DNA Fingerprinting / Q000379:methods*; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences
D005819:Genetic Markers