title: In vitro translation.
abstract: The two most frequently used systems for in vitro translation are the rabbit reticulocyte system and the wheat germ extract. These systems are useful for mRNAs isolated from cells, tissues, and capped or uncapped mRNA produced in vitro by transcription of cDNA. In a combined system, mRNA can be transcribed and translated in a single reaction. In addition these systems can be used for translation reactions with biotinylated amino acids; this allows capture of the newly synthesized protein using streptavidin immobilized on agarose.
authors.affiliation: Center of Marine Biotechnology and Greenebaum Cancer Center, Baltimore, Maryland, USA.
authors.forename: R
authors.initials: R
authors.lastname: Jagus
authors.forename: B
authors.initials: B
authors.lastname: Joshi
authors.forename: S
authors.initials: S
authors.lastname: Miyamoto
authors.forename: G S
authors.initials: GS
authors.lastname: Beckler
mesh_terms: D000818:Animals; D002474:Cell-Free System; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008967:Molecular Biology / Q000379:methods*; D010936:Plant Extracts; D012333:RNA, Messenger / Q000502:physiology; D011817:Rabbits; D012156:Reticulocytes / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*; D014908:Triticum
chemical_list: D010936:Plant Extracts; D012333:RNA, Messenger