title: Antioxidants attenuate oxidative damage in rat skeletal muscle during mild ischaemia.
abstract: We have previously shown oxidative stress and oedema, caused by both xanthine oxidase-derived oxidants and infiltrating neutrophils, within skeletal muscle after contractile-induced claudication. The purpose of this study was to determine whether supplementation with antioxidant vitamins attenuates the oxidative stress, neutrophil infiltration and oedema associated with an acute bout of contractile-induced claudication. Rats received vehicle, vitamin C, vitamin E or vitamin C + E for 5 days prior to contractile-induced claudication. Force production was significantly reduced in the claudicant limbs of all groups compared with the control (sham) limb of control animals. Contractile-induced claudication caused a significant increase in protein oxidation, lipid peroxidation, neutrophil infiltration and oedema compared with sham muscles. Supplementation with vitamin C, E or C + E prevented the increases in each of these, and there were no differences between groups. These findings suggest that, in an animal model of exercise-induced claudication, neutrophil chemotaxis is caused by oxidizing species and that antioxidant supplementation can prevent oxidative damage, neutrophil infiltration and oedema following an acute bout of contractile-induced claudication.
authors.affiliation: Muscle Physiology Laboratory, Department of Applied Physiology, University of Florida, Gainesville, FL 32611, USA. ajudge@hhp.ufl.edu
authors.forename: A R
authors.initials: AR
authors.lastname: Judge
authors.forename: J T
authors.initials: JT
authors.lastname: Selsby
authors.forename: S L
authors.initials: SL
authors.lastname: Dodd
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004487:Edema / Q000209:etiology / Q000517:prevention & control; D004558:Electric Stimulation; D007383:Intermittent Claudication / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007511:Ischemia / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D020556:Neutrophil Infiltration / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000378:metabolism; D050050:Protein Carbonylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D014810:Vitamin E / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D009195:Peroxidase; D001205:Ascorbic Acid