title: Culture of subconfluent human fibroblasts and keratinocytes using biodegradable transfer membranes.
abstract: This study aims to assess the suitability of biodegradable membranes as transfer matrix materials for the culture of subconfluent fibroblasts and keratinocytes. The materials investigated were based on collagen, chitosan and enzyme-digestible cellulose. The proliferation and growth behaviour of human keratinocytes and dermal fibroblasts were analysed and morphology and distribution determined. Cultured fibroblasts exhibited no significant differences in proliferation for the different membrane types, whereas keratinocytes revealed significantly higher proliferation on collagen membranes compared with membranes based on cellulose and chitosan. Co-cultured fibroblasts and keratinocytes from the same donor on collagen membranes showed more homogenous cell distribution, but they segregated in heterologous co-cultures; this effect must be further investigated. Thus, collagen and collagen-coated chitosan membranes are suitable for the subconfluent transfer of human fibroblasts and keratinocytes.
authors.affiliation: Charité, Campus Virchow-Clinic, Department of Surgery, Universitätsmedizin Berlin, Germany. christa.johnen@charite.de
authors.forename: C
authors.initials: C
authors.lastname: Johnen
authors.forename: I
authors.initials: I
authors.lastname: Steffen
authors.forename: D
authors.initials: D
authors.lastname: Beichelt
authors.forename: K
authors.initials: K
authors.lastname: Bräutigam
authors.forename: T
authors.initials: T
authors.lastname: Witascheck
authors.forename: N
authors.initials: N
authors.lastname: Toman
authors.forename: V
authors.initials: V
authors.lastname: Moser
authors.forename: C
authors.initials: C
authors.lastname: Ottomann
authors.forename: B
authors.initials: B
authors.lastname: Hartmann
authors.forename: J C
authors.initials: JC
authors.lastname: Gerlach
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002482:Cellulose; D048271:Chitosan; D018920:Coculture Techniques; D003094:Collagen; D005347:Fibroblasts / Q000166:cytology* / Q000637:transplantation; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000637:transplantation; D008422:Materials Testing / Q000379:methods; D008567:Membranes, Artificial; D012867:Skin / Q000166:cytology*; D016038:Skin Transplantation / Q000379:methods; D017768:Skin, Artificial
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D002482:Cellulose; D003094:Collagen; D048271:Chitosan