Requirement of B2-type cyclin-dependent kinases for meristem integrity in Arabidopsis thaliana.
To maintain proper meristem function, cell division and differentiation must be coordinately regulated in distinct subdomains of the meristem. Although a number of regulators necessary for the correct organization of the shoot apical meristem (SAM) have been identified, it is still largely unknown how their function is integrated with the cell cycle machinery to translate domain identity into correct cellular behavior. We show here that the cyclin-dependent kinases CDKB2;1 and CDKB2;2 are required both for normal cell cycle progression and for meristem organization. Consistently, the CDKB2 genes are highly expressed in the SAM in a cell cycle-dependent fashion, and disruption of CDKB2 function leads to severe meristematic defects. In addition, strong alterations in hormone signaling both at the level of active hormones and with respect to transcriptional and physiological outputs were observed in plants with disturbed CDKB2 activity.
Andersen
Stig Uggerhøj
SU
Max Planck Institute for Developmental Biology, AG Lohman, D-72076, Tübingen, Germany.
Buechel
Sabine
S
Zhao
Zhong
Z
Ljung
Karin
K
Novák
Ondrej
O
Busch
Wolfgang
W
Schuster
Christoph
C
Lohmann
Jan U
JU
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018519:Meristem / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D015398:Signal Transduction / Q000187:drug effects
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D010937:Plant Growth Regulators; C518664:CDKB2;1 protein, Arabidopsis; C435713:CDKb2;2 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases