Determination of Akt/PKB signaling.
This unit provides a basic protocol for assaying the level of activity, as well as the activation capacity and dynamics of inhibition of Akt/PKB in cultured cells. All these data can be obtained in a single nonradioactive experiment by standard techniques including immunoblotting and immunodetection with phosphospecific antibodies. This unit also provides a support protocol for assaying the membrane translocation of Akt.
Pankov
Roumen
R
National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
D000818:Animals; D001693:Biological Transport, Active; D002470:Cell Survival; D002478:Cells, Cultured; D008722:Methods; D051379:Mice; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction
D051057:Proto-Oncogene Proteins c-akt