Genetic portrait of osteoblast-like cells cultured on PerioGlas.
PerioGlas (PG) is an alloplastic material used for grafting periodontal osseous defects since 1995. In animal models it has been histologically proven that PG achieves good repair of surgically created defects. In clinical trials, PG has been shown to be effective as an adjunct to conventional surgery in treating intrabony defects. Because the molecular events by which PG is able to alter osteoblast activity to promote bone formation are poorly understood, we investigated genes that are differently regulated in osteoblast-like cells exposed to PG. Bone formation can be attributable to ostegenesis (ie, direct stimulation of osteoblast to produce new bone), osteoconduction (which operates like a scaffold), or both processes. By using DNA microarrays containing 20 000 oligonucleotides, we identified several genes in which expression was significantly downregulated in a MG63 cell line cultured with PerioGlas (US Biomaterials Corp, Alachua, Fla). Specifically, PG is able to downregulate some functional activities of osteoblast-like cells: it acts on signal transduction, especially on the transforming growth factor beta (TGFB) paracrine network; it inhibits apoptosis; it decreases cell adhesion with consequent enhancement of cell mobility and migration; and it acts on bone marrow stem cells (ie, CD34). In conclusion, PG acts on bone formation by determining both osteoconduction (as demonstrated by the reduced cell adhesion) and ostegenesis (as shown by TGFB-related proteins and stem cell markers).
Carinci
Francesco
F
Department of Embryology and Morphology, University of Ferrara.
Palmieri
Annalisa
A
Martinelli
Marcella
M
Perrotti
Vittoria
V
Piattelli
Adriano
A
Brunelli
Giorgio
G
Arlotti
Marzia
M
Pezzetti
Furio
F
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D018786:Bone Substitutes / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D002516:Ceramics / Q000494:pharmacology*; D016172:DNA Fingerprinting; D018076:DNA, Complementary / Q000032:analysis; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D012313:RNA / Q000032:analysis; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
C022107:Bioglass; D018786:Bone Substitutes; D018076:DNA, Complementary; D012313:RNA