Parameters affecting electro membrane extraction of basic drugs.
Thirty-five different basic drugs were extracted by electro membrane extraction (EME), from acidified samples containing HCl as the BGE, through an organic solvent immobilized in the pores in the wall of a porous hollow fiber (supported liquid membrane, SLM), and into an acidified acceptor solution (HCl) in the lumen of the hollow fiber by the application of an electrical potential difference of 50 V. With 2-nitrophenyl pentyl ether (NPPE) as the SLM, and with 10 mM HCl as BGE in the sample and acceptor solution, singly charged basic drugs with log P >2 were extracted with recoveries in the range 30-81% within 5 min. For doubly charged basic drugs, extraction was effectively enhanced by decreasing the concentration of HCl in the sample from 10 to 0.1 mM, reducing the ionization of the analytes. For medium polar analytes (1 < log P < 2), an ion balance of 0.01 was combined with addition of tris-(2-ethylhexyl) phosphate (TEHP) to the SLM, and this provided recoveries in the range 36-70%. The ion balance was defined as the concentration ratio of BGE between the sample and the acceptor solution. For the most polar drugs (log P <1), EME was accomplished with an ion balance of 0.01 and with di-(2-ethylhexyl) phosphate (DEHP) added to the SLM, but in spite of this, recoveries were in the range of only 4-17%.
Middelthon-Bruer
Torunn M
TM
School of Pharmacy, University of Oslo, Blindern, Oslo, Norway.
Gjelstad
Astrid
A
Rasmussen
Knut E
KE
Pedersen-Bjergaard
Stig
S
D004563:Electrochemistry; D008567:Membranes, Artificial; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*
D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations