Fluconazole release from hydrogels including acrylamide-acrylic acid-itaconic acid, and their microbiological interactions.
Polyacrylamide (PAAm), polyacrylic acid (PAA), poly(acrylamide-co-itaconic acid) (PAAmIA) and poly(acrylic acid-co-itaconic acid) (PAAIA) hydrogels were prepared via free-radical polymerization using ethylene glycol dimethacrylate (EGDMA) as cross-linker. The variations of swelling percentages with time and pH were determined for these hydrogels at 37 degrees C. PAAmIA was found as the most swollen hydrogel at pH 4.0. SEM micrographs were taken to observe the morphology of the hydrogels. The less swollen hydrogel, PAAIA, displays less porosity relative to PAAmIA hydrogel. Fluconazole was entrapped into PAAmIA and PAAIA hydrogels and the release was investigated in Britton-Robinson buffer solution (BR) at pH 4.0 and 37 degrees C. The kinetic release parameters of the hydrogels, n and k, were calculated and Fickian-type diffusion was established for PAAmIA, which releases Fluconazole faster than PAAIA hydrogel. Therapeutic range was reached in the first hour for both hydrogels. Microbiological interactions of hydrogels were also studied in vitro in vaginal medium. It is found that Fluconazole entrapped in hydrogels inhibited the growth of Candida albicans.
Pulat
Mehlika
M
Gazi Universitesi Fen, Edebiyat Fakültesi, Kimya Bölümü, Teknikokullar, Ankara, Turkey. mpulat@gazi.edu.tr
Eksi
Haslet
H
Abbasoglu
Ufuk
U
D020106:Acrylamide / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D004337:Drug Carriers; D015725:Fluconazole / Q000737:chemistry* / Q000494:pharmacology*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D013386:Succinates / Q000737:chemistry*
D000179:Acrylates; D000180:Acrylic Resins; D000935:Antifungal Agents; D004337:Drug Carriers; D020100:Hydrogels; D007202:Indicators and Reagents; D013386:Succinates; D020106:Acrylamide; C006903:carbopol 940; D015725:Fluconazole; C036658:acrylic acid; C005229:itaconic acid