Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion.
The aim of this study was to compare the outcomes of nerve autografts (GRF) and venous grafts containing mononuclear bone marrow cells (BMCs) in sciatic nerve-lesioned rats. Control animals underwent sham operations (SHAM), received empty venous grafts (EPV), or received venous grafts containing BMC vehicle (AGR). Outcome was evaluated through sciatic functional index (SFI), morphometric and morphologic analyses of the nerve distal to the lesion, and the number of spinal cord motor neurons positive for the retrograde tracer, Fluoro-Gold. All groups exhibited poor results in SFI when compared to SHAM animals throughout the postoperative period. All groups also had a significantly greater fiber density, decreased fiber diameter, and decreased motor neuron number than the SHAM group. No significant difference between the GRF and BMC groups was observed in any of these parameters. On the other hand, vessel density was significantly higher in BMC than all other groups. BMC-containing venous grafts are superior to nerve autografts in increasing vessel density during sciatic nerve regeneration.
Fernandes
Marcela
M
Department of Orthopedics and Traumatology, Federal University of São Paulo, Rua Borges Lagoa 786, São Paulo, Brazil. fernandesmarcela@click21.com
Valente
Sandra Gomes
SG
Fernandes
Maria José da Silva
MJ
Félix
Evandro Penteado Villar
EP
Mazzacoratti
Maria da Graça Naffah
Mda G
Scerni
Débora Amado
DA
dos Santos
João Baptista Gomes
JB
Leite
Vilnei Mattioli
VM
Faloppa
Flávio
F
D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000637:transplantation; D001854:Bone Marrow Cells / Q000502:physiology*; D002452:Cell Count; D005456:Fluorescent Dyes; D008297:Male; D009186:Myelin Sheath / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009412:Nerve Fibers / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D012584:Sciatic Nerve / Q000293:injuries* / Q000502:physiology; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines