Production of papillomavirus-based gene transfer vectors.
Papillomaviruses are a diverse group of pathogens that infect the skin and mucosal tissues of humans and various animal species. The viral genome is a circular, double-stranded DNA molecule approximately 8-kb in length. The non-enveloped papillomavirus capsid is composed of a virally encoded major coat protein, L1, and a minor coat protein, L2. L1 and L2 co-assemble when expressed in mammalian cells, and can promiscuously encapsidate essentially any <8-kb plasmid present in the cell nucleus. In the last several years, there has been rapid development of techniques for intracellular production of papillomavirus-based gene transfer vectors (also known as pseudoviruses). This unit outlines the production and propagative amplification of papillomaviral vectors. The system represents a highly tractable method for converting pre-existing mammalian expression plasmids into infectious virus stocks. The resulting vectors have utility for in vitro, as well as in vivo gene delivery applications.
Buck
Christopher B
CB
National Cancer Institute, Center for Cancer Research, Bethesda, Maryland, USA.
Thompson
Cynthia D
CD
D000818:Animals; D004279:DNA, Viral; D018014:Gene Transfer Techniques / Q000639:trends; D015316:Genetic Therapy / Q000639:trends; D005822:Genetic Vectors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000235:genetics
D004279:DNA, Viral