title: Optimal method of collection of first-void urine for diagnosis of Chlamydia trachomatis infection in men.
abstract: First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup by quantitative PCR (32,533 versus 5,271 plasmids/ml; P < 0.0001). Consequently, the use of FirstBurst to collect a urine sample improved the sensitivity of a rapid test for Chlamydia over testing of samples collected with a urine cup (82 versus 47% sensitivity using PCR as a reference; P < 0.0015).
authors.affiliation: Department of Haematology, University of Cambridge, EABC Site, Long Road, Cambridge CB2 2PT, United Kingdom.
authors.forename: Craig A
authors.initials: CA
authors.lastname: Wisniewski
authors.forename: John A
authors.initials: JA
authors.lastname: White
authors.forename: Claude-Edouard C
authors.initials: CE
authors.lastname: Michel
authors.forename: Lourdes
authors.initials: L
authors.lastname: Mahilum-Tapay
authors.forename: Jose Paolo V
authors.initials: JP
authors.lastname: Magbanua
authors.forename: Elpidio Cesar B
authors.initials: EC
authors.lastname: Nadala
authors.forename: Penelope J
authors.initials: PJ
authors.lastname: Barber
authors.forename: Beng T
authors.initials: BT
authors.lastname: Goh
authors.forename: Helen H
authors.initials: HH
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods*; D014556:Urine / Q000382:microbiology*