title: Association between the -2518G/A polymorphism in the monocyte chemoattractant protein-1 (MCP-1) gene and myocardial infarction in Tunisian patients.
abstract: BACKGROUND
  Monocyte chemoattractant protein-1 (MCP-1; gene name CCL2) has been suggested to play an important role in the initiation of atherosclerosis by recruiting monocytes to sites of injured endothelium. Recently, single nucleotide polymorphisms (SNPs) in the MCP-1 regulatory region have been identified. Controversial results regarding the association of the -2518G/A polymorphism of the MCP-1 gene with coronary artery disease (CAD) have been reported. In the present study, we examined a possible association between the -2518G/A polymorphism of the MCP-1 gene and myocardial infarction (MI) in a sample of the Tunisian population.
  
  
  METHODS
  A total of 319 Tunisian patients with MI and 467 healthy controls were included in the study. The SNP of the MCP-1 gene was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.
  
  
  RESULTS
  Patients with MI had significantly higher frequency of the AG+GG genotypes compared to controls [42.9% vs. 35.8%; OR (95%CI), 1.34 (1.00-1.79); p=0.04]. The MI patient group showed a significant higher frequency of the G allele compared to the controls [0.242 vs. 0.195; OR (95%CI), 1.31(1.02-1.68), p=0.03]. The association between the -2518G/A polymorphism of the MCP-1 gene and MI was no longer significant after adjustment for other well-established risk factors.
  
  
  CONCLUSIONS
  The present study showed a significant but not independent association between the -2518G/A polymorphism of the MCP-1 gene (presence of G allele) and MI in the Tunisian population.
authors.affiliation: Research Laboratory LAB-SM-01, Department of Biochemistry, Hospital la Rabta, Tunis, Tunisia. jemaa_riadh@yahoo.fr
authors.forename: Riadh
authors.initials: R
authors.lastname: Jemaa
authors.forename: Hajer
authors.initials: H
authors.lastname: Rojbani
authors.forename: Amani
authors.initials: A
authors.lastname: Kallel
authors.forename: Samir
authors.initials: S
authors.lastname: Ben Ali
authors.forename: Moncef
authors.initials: M
authors.lastname: Feki
authors.forename: Sonia
authors.initials: S
authors.lastname: Chabrak
authors.forename: Monia
authors.initials: M
authors.lastname: Elasmi
authors.forename: Samah Haj
authors.initials: SH
authors.lastname: Taieb
authors.forename: Haïfa
authors.initials: H
authors.lastname: Sanhaji
authors.forename: Omar
authors.initials: O
authors.lastname: Souheil
authors.forename: Rachid
authors.initials: R
authors.lastname: Mechmeche
authors.forename: Naziha
authors.initials: N
authors.lastname: Kaabachi
mesh_terms: D001483:Base Sequence; D018932:Chemokine CCL2 / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D014416:Tunisia
chemical_list: D018932:Chemokine CCL2; D017931:DNA Primers