title: Dominant suppression of repeat-induced point mutation in Neurospora crassa by a variant catalytic subunit of DNA polymerase zeta.
abstract: Crosses involving the Adiopodoumé strain of Neurospora crassa are defective for repeat-induced point mutation (RIP), a genome defense mechanism of fungi. We show here that the Adiopodoumé strain possesses an incompletely penetrant and variably expressive dominant suppressor of RIP (Srp) that maps to an approximately 34-kbp genome segment that is approximately 26 kbp proximal to mat on linkage group IL. Gene disruption experiments revealed that Srp is the upr-1 allele of Adiopodoumé (upr-1(Ad)) that is contained within this segment. The upr-1 gene codes for the catalytic subunit of the translesion DNA polymerase-zeta (Pol-zeta) and it is unusually polymorphic in Neurospora. That the upr-1 gene contains upstream ORFs that overlap with the main ORF is potentially relevant to the incomplete penetrance and variable expressivity of the suppressor. Crosses between heterokaryons that contain upr-1(Ad) and strains that prevent mating events involving nuclei that contain upr-1(Ad) yielded no progeny in which RIP had occurred, consistent with the idea that the suppressor encoded by upr-1(Ad) is diffusible. The potential involvement of the Pol-zeta subunit in two functions, translesion DNA synthesis and RIP regulation, might account for the rapid evolution of its gene in Neurospora.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
authors.forename: Ranjan
authors.initials: R
authors.lastname: Tamuli
authors.forename: Durgadas P
authors.initials: DP
authors.lastname: Kasbekar
mesh_terms: D000483:Alleles; D020134:Catalytic Domain / Q000235:genetics*; D015825:Chromosomes, Fungal / Q000235:genetics; D003433:Crosses, Genetic; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005799:Genes, Dominant; D016153:Genes, Suppressor; D006720:Homozygote; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics*; D019683:Penetrance; D010641:Phenotype; D017354:Point Mutation / Q000235:genetics*; D013489:Suppression, Genetic
chemical_list: C425929:DNA polymerase zeta; D004259:DNA-Directed DNA Polymerase