Intranuclear nucleolin distribution during cell cycle progression in human invasive ductal breast carcinomas in relation to estrogen receptor status.
BACKGROUND
Nucleolin (NU) can be found in the nucleus in aggregates and in a diffused form. The expression and distribution of these two intranuclear pools of nucleolin was compared in estrogen receptor (ER)-positive and -negative invasive ductal breast carcinomas in relation to cell cycle phases.


METHODS
Using a laser scanning cytometer NU was measured within the whole nucleus, within nucleolin aggregates (NUA) and within the NUA-free karyoplasm. The G1-, S- and G2M-phase cell subpopulations were distinguished on DNA histograms.


RESULTS
The nuclei of ER-positive and ER-negative breast carcinomas in the G2M-phase differed in the expression of NU in the NUA and in the NUA-free karyoplasm, whereas in the S-phase they only differed in the NUA-free karyoplasm expression. In ER-positive carcinomas NU levels rose from G1 to G2M in both the NUA and the NUA-free karyoplasm, whereas in ER-negative carcinomas the NU levels increased only between G1/S in the NUA and between S/G2M in the NUA-free karyoplasm.


CONCLUSIONS
There are significant differences in nucleolin expression and localization during cell cycle progression depending on ER status.
Masiuk
Marek
M
Department of Pathology, Pomeranian Medical University, 71-252 Szczecin, Poland. mmasiuk@med.pam.szczecin.pl
Urasinska
Elzbieta
E
Domagala
Wenancjusz
W
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology*; D002453:Cell Cycle / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D006801:Humans; D010750:Phosphoproteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000096:biosynthesis*; D000097796:Nucleolin
D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D011960:Receptors, Estrogen; D000097796:Nucleolin