[Study on extraction technology of Mosla chinensis volatile oil].
OBJECTIVE
To study the techniques of organic solvent extraction method and supercritical CO2 extraction for extracting Mosla chinensis Maxim volatile oil (MCMVO), and to analyze its chemical compositions.


METHODS
Organic solvent extraction method and supercritical CO2 extraction were used to extract the oil and GC-MS was used to analyze its chemical compositions.


RESULTS
The best extraction techniques of organic solvent method to extract volatile oil should be divided into two steps, total time was 210 minutes, the total proportions of input of the solvent and raw materials was 8:1 (ml:g), and temprature was 65 degrees C, its extraction rate was 1.83%. The MCMVO was extracted by supercritical CO2, and its extraction rate was 3.4%. GC-MS was used to analyze volatile oil, the results suggested MCMVO contain thymol, carvacrol, P-cycmene, humulene etc. The thymol had the highest content accounts for 56.25%, and the carvacrol was 19.21%. The content of thymol and carvacrol of MCMVO extracted by organic solvent was 58.33% and 22.54%.


CONCLUSIONS
the content of thymol and carvacrol extracted by organic solvent extraction method is higher than that extracted by supercritical CO2 extraction method. Chemical compositions extracted by organic solvent extraction method are less than extracted by supercritical CO2 extraction method. 10 compositions are firstly extracted by supercritical CO2 extraction method.
Jiang
Hong-mei
HM
College of Science, Hunan Agricultural University, Changsha 410028, China. jianghongmei730423@yahoo.com.cn
Lu
Xiang-yang
XY
Fang
Jun
J
Xu
Xiang-li
XL
Yi
Ke
K
Ge
Bing
B
D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods; D000081007:Cymenes; D008401:Gas Chromatography-Mass Spectrometry; D019686:Lamiaceae / Q000737:chemistry*; D015394:Molecular Structure; D039821:Monoterpenes / Q000032:analysis / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013943:Thymol / Q000032:analysis / Q000737:chemistry; D013997:Time Factors
D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D012997:Solvents; D002245:Carbon Dioxide; D013943:Thymol; C073316:carvacrol