Redox state and gender differences in vascular smooth muscle cells.
Vascular smooth muscle cells (VSMC) have been isolated from male and female rat aorta and studied to assess their susceptibility to ultraviolet radiation-induced oxidative stress. Interestingly, a gender difference, in terms of reactive oxygen species production, was detected in both basal and irradiated VSMC. Namely, VSMC from male rats were more susceptible to radiation-induced stress and easier underwent apoptosis in comparison to cells from female rats. Conversely, the latter, in the same experimental conditions, clearly displayed signs of premature senescence. These results indicate that a sort of "gender memory" can be conserved in VMSC in primary culture.
Malorni
Walter
W
Department of Drug Research and Evaluation, Section of Cell Aging and Degeneration, Istituto Superiore di Sanita', Viale Regina Elena 299, Rome, Italy. malorni@iss.it
Straface
Elisabetta
E
Matarrese
Paola
P
Ascione
Barbara
B
Coinu
Rita
R
Canu
Silvia
S
Galluzzo
Paola
P
Marino
Maria
M
Franconi
Flavia
F
D000199:Actins / Q000378:metabolism; D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D015153:Blotting, Western; D019070:Cell Lineage; D016922:Cellular Senescence; D003599:Cytoskeleton / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D008297:Male; D008856:Microscopy, Fluorescence; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011419:Propidium / Q000378:metabolism; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D011944:Receptors, Androgen / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D012727:Sex Characteristics
D000199:Actins; D017304:Annexin A5; D017382:Reactive Oxygen Species; D011944:Receptors, Androgen; D011960:Receptors, Estrogen; D011419:Propidium