Integration of various stacking processes in carrier ampholyte-based capillary electrophoresis.
Field-enhanced sample stacking, field-enhanced sample injection as well as electrokinetic supercharging have been successfully integrated in carrier ampholyte-based capillary electrophoresis. Through the analysis of different test sample mixtures, it has been shown that the carrier ampholyte-based background electrolytes, in spite of their very low conductivity, allow efficient online preconcentration of analytes by field-amplified techniques. Sensitivity enhancement factors of the same magnitude as those usually encountered with classical conductive background electrolytes have been obtained in such carrier ampholyte-based buffers. Depending on the online preconcentration method that has been integrated, sensitivity enhancement factors between 50 and several thousands have been reached.
Busnel
Jean-Marc
JM
Laboratoire Environnement et Chimie Analytique, UMR CNRS 7121, ESPCI, 10 rue Vauquelin, 75005 Paris, France. jean-marc.busnel@epfl.ch
Le Saux
Thomas
T
Descroix
Stéphanie
S
Girault
Hubert H
HH
Hennion
Marie-Claire
MC
Terabe
Shigeru
S
Peltre
Gabriel
G
D000665:Ampholyte Mixtures; D000818:Animals; D002021:Buffers; D045304:Cytochromes c / Q000032:analysis*; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006321:Heart; D006736:Horses; D007525:Isoelectric Focusing / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity
D000665:Ampholyte Mixtures; D002021:Buffers; D004573:Electrolytes; D010455:Peptides; D045304:Cytochromes c