Reactive oxygen species metabolism during the cadmium hyperaccumulation of a new hyperaccumulator Sedum alfredii (Crassulaceae).
Sedum alfredii Hance, a newly discovered hyperaccumulator, could serve as a good material for phytoremediation of Cd polluted sites. Malondialdehyde (MDA), reactive oxygen species (ROS) and antioxidases (catalase (CAT); superoxide dismutase (SOD); peroxidase (POD)) in the leaf were determined when S. alfredii was treated for 15 d with various CdCl2 concentrations ranging from 0 to 800 micromol/L. The results showed that the production rate of 2',7'-dichlorofluorescein (DCF), which is an indicator of ROS level, reached up to the maximum at 400 micromol/L CdCl2 and then declined with the increase of CdCl2 concentration, while MDA accumulation tended to increase. CAT activity was significantly inhibited at all tested CdCl2 concentrations and SOD activity was sharply suppressed at 800 micromol/L CdCl2. However, the enhancement of POD activity was observed when CdCl2 concentration was higher than 400 micromol/L. In addition, its activity increased when treated with 600 micromol/L CdCl2 for more than 5 d. When sodium benzoate, a free radical scavenger, was added, S. alfredii was a little more sensitive to Cd toxicity than that exposed to Cd alone, and the Cd accumulation tended to decline with the increase of sodium benzoate concentration. It came to the conclusions that POD played an important role during Cd hyperaccumulation, and the accumulation of ROS induced by Cd treatment might be involved in Cd hyperaccumulation.
Zhang
Zhong-chun
ZC
College of Life Sciences, Huazhong Normal University, Wuhan 430079, China. zzchun19820102@126.com
Qiu
Bao-Sheng
BS
D001673:Biodegradation, Environmental; D019256:Cadmium Chloride / Q000378:metabolism* / Q000633:toxicity; D002374:Catalase / Q000037:antagonists & inhibitors / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D031963:Sedum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D020160:Sodium Benzoate / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013997:Time Factors
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D019256:Cadmium Chloride; D020160:Sodium Benzoate