[Different effect of galanin on the proliferation of PC12 and B104 cells].
The effect of galanin (GAL) on neural proliferation was studied in this article using PC12 and B104 cells. RT-PCR was used to determine the expression of galanin and its receptors in both cells. MTT analysis method was employed to detect the effects of galanin and the agonist, antagonist of galanin receptors on the proliferation of both cells. Results showed that PC12 cells expressed mRNAs for all the three galanin receptors (GalRs) but not for galanin, while B104 cells expressed galanin, GalR2, GalR3 except GalR1. In addition, galanin and two receptor agonists, GAL-11 and GAL2-11, could inhibit the proliferation of PC12 cells but activated the proliferation of B104 cells significantly. Moreover, the influences could be blocked by M35, a nonspecific antagonist of galanin receptors. It suggested that GAL can affect cell proliferation via GalRs, but the different galanin receptors might mediate different cell functions.
Cheng
Shuang
S
School of Life Science of East China Normal University, Shanghai 200062, China.
Yuan
Chong Gang
CG
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019004:Galanin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D016716:PC12 Cells; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D044088:Receptors, Galanin / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
D012333:RNA, Messenger; D044088:Receptors, Galanin; D019004:Galanin