Metabolic flux analysis in biotechnology processes.
Metabolic flux analysis (MFA) has become a fundamental tool of metabolic engineering to elucidate the metabolic state of the cell and has been applied to various biotechnological processes. In recent years, considerable technical advances have been made. Developments of analytical instruments allow us to determine (13)C labeling distribution of intracellular metabolites with high accuracy and sensitivity. Moreover, kinetic information of intracellular label distribution during isotopic instationary enables us to calculate metabolic fluxes with shortened experimental time and decreased amount of labeled substrate. The (13)C MFA may be one of the most promising approaches for the target estimation to improve strain performances and production processes.
Iwatani
Shintaro
S
Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc, Kawasaki-shi, Kanagawa, 210-8681, Japan. shintaro_iwatani@ajinomoto.com
Yamada
Yohei
Y
Usuda
Yoshihiro
Y
D000596:Amino Acids / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D002247:Carbon Isotopes / Q000378:metabolism*; D019295:Computational Biology; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D010969:Plastics / Q000378:metabolism; D014815:Vitamins / Q000096:biosynthesis
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D002247:Carbon Isotopes; D010969:Plastics; D014815:Vitamins; D000431:Ethanol; D005990:Glycerol