Deposits of transforming growth factor-beta-induced protein in granular corneal dystrophy type II after LASIK.
OBJECTIVE
To analyze components of the deposits in the corneal flap interface of granular corneal dystrophy type II (GCD II) patients after laser in situ keratomileusis (LASIK).


METHODS
Four corneal GCD II specimens displaying disease exacerbation after LASIK were analyzed. Three of these specimens included the recipient corneal button after penetrating keratoplasty or deep lamellar keratoplasty for advanced GCD II after LASIK. The fourth specimen, a similar case of GCD II after LASIK, included the amputated corneal flap. Specimens were processed for histopathologic and immunohistochemical analyses.


RESULTS
Corneal stromal deposits in the LASIK flaps of all specimens were stained with 3 anti-transforming growth factor-beta-induced protein (TGFBIp) antibodies. The deposits displayed bright red color staining with Masson trichrome; however, negative staining was seen with Congo red, suggesting that hyaline is the main component localizing to the TGFBIp deposits rather than amyloid.


CONCLUSIONS
Amorphous granular material deposited along the interface of the LASIK flap in GCD II corneas is composed mainly of hyaline deposits.
Kim
Tae-Im
TI
Corneal Dystrophy Research Institute, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
Roh
Mi In
MI
Grossniklaus
Hans E
HE
Kang
Shin Jeong
SJ
Hamilton
Stephen M
SM
Schorderet
Daniel F
DF
Lee
W Barry
WB
Kim
Eung Kweon
EK
D000328:Adult; D001391:Azo Compounds; D004396:Coloring Agents; D003224:Congo Red; D003317:Corneal Dystrophies, Hereditary / Q000378:metabolism* / Q000601:surgery; D003319:Corneal Stroma / Q000378:metabolism*; D004801:Eosine Yellowish-(YS); D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D020731:Keratomileusis, Laser In Situ; D015948:Keratoplasty, Penetrating; D008297:Male; D008739:Methyl Green; D008875:Middle Aged; D013194:Staining and Labeling / Q000379:methods; D013524:Surgical Flaps; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D000099222:betaIG-H3 Protein
D001391:Azo Compounds; D004396:Coloring Agents; D003224:Congo Red; D004801:Eosine Yellowish-(YS); D016326:Extracellular Matrix Proteins; D008739:Methyl Green; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C017768:trichrome stain