title: [Genetic diversity of wild Cynodon dactylon germplasm detected by SRAP markers].
abstract: Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity of 32 wild accessions of Cynodon dactylon collected from Sichuan, Chongqing, Guizhou and Tibet, China. The following results were obtained. (1) Fourteen primer pairs produced 132 polymorphic bands, averaged 9.4 bands per primer pair. The percentage of polymorphic bands in average was 79.8%. The Nei's genetic similarity coefficient of the tested accessions ranged from 0.591 to 0.957, and the average Nei's coefficient was 0.759. These results suggested that there was rich genetic diversity among the wild resources of Cynodon dactylon tested. (2) Thirty two wild accessions were clustered into four groups. Moreover, the accessions from the same origin frequently clustered into one group. The findings implied that a correlation among the wild resources, geographical and ecological environment. (3) Genetic differentiation between and within six eco-geographical groups of C. dactylon was estimated by Shannon's diversity index, which showed that 65.56% genetic variance existed within group, and 34.44% genetic variance was among groups. (4) Based on Nei's unbiased measures of genetic identity, UPGMA cluster analysis measures of six eco-geographical groups of Cynodon dactylon, indicated that there was a correlation between genetic differentiation and eco-geographical habits among the groups.
authors.affiliation: Department of Grassland, Sichuan Agricultural University, Ya'an 625014, China. yyj_914@yahoo.com.cn
authors.forename: Yang-Jie
authors.initials: YJ
authors.lastname: Yi
authors.forename: Xin-Quan
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Lin-Kai
authors.initials: LK
authors.lastname: Huang
authors.forename: Yao
authors.initials: Y
authors.lastname: Ling
authors.forename: Xiao
authors.initials: X
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D031744:Cynodon / Q000166:cytology / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D010375:Pedigree; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers