title: Mechanical regulation of osteoclastic genes in human osteoblasts.
abstract: Bone adaptation to mechanical load is accompanied by changes in gene expression of bone-forming cells. Less is known about mechanical effects on factors controlling bone resorption by osteoclasts. Therefore, we studied the influence of mechanical loading on several key genes modulating osteoclastogenesis. Human osteoblasts were subjected to various cell stretching protocols. Quantitative RT-PCR was used to evaluate gene expression. Cell stretching resulted in a significant up-regulation of receptor activator of nuclear factor-kappaB ligand (RANKL) immediate after intermittent loading (3x3h, 3x6h, magnitude 1%). Continuous loading, however, had no effect on RANKL expression. The expression of osteoprotegerin (OPG), macrophage-colony stimulating factor (M-CSF), and osteoclast inhibitory lectin (OCIL) was not significantly altered. The data suggested that mechanical loading could influence osteoclasts recruitment by modulating RANKL expression in human osteoblasts and that the effects might be strictly dependent on the quality of loading.
authors.affiliation: Institute of Orthopaedic Research and Biomechanics, Centre of Musculoskeletal Research, University of Ulm, Helmholtzstrasse 14, D-89081 Ulm, Germany.
authors.forename: Ludwika
authors.initials: L
authors.lastname: Kreja
authors.forename: Astrid
authors.initials: A
authors.lastname: Liedert
authors.forename: Sofia
authors.initials: S
authors.lastname: Hasni
authors.forename: Lutz
authors.initials: L
authors.lastname: Claes
authors.forename: Anita
authors.initials: A
authors.lastname: Ignatius
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D005246:Feedback / Q000502:physiology; D005260:Female; D006801:Humans; D037181:Lectins, C-Type; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010010:Osteoclasts / Q000502:physiology*; D053244:Osteoprotegerin / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism*; D011956:Receptors, Cell Surface; D013314:Stress, Mechanical
chemical_list: C400041:CLEC2D protein, human; D037181:Lectins, C-Type; D053244:Osteoprotegerin; D053245:RANK Ligand; D011956:Receptors, Cell Surface; C505688:TNFSF11 protein, human; D016173:Macrophage Colony-Stimulating Factor