title: Basic techniques in mammalian cell tissue culture.
abstract: Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.
authors.affiliation: Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Phelan
mesh_terms: D000818:Animals; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured / Q000166:cytology; D015925:Cryopreservation / Q000379:methods; D003470:Culture Media; D006801:Humans; D007202:Indicators and Reagents; D008322:Mammals / Q000033:anatomy & histology*; D013535:Suspensions; D014186:Transportation
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D013535:Suspensions