Strongyloides venezuelensis: the antigenic identity of eight strains for the immunodiagnosis of human strongyloidiasis.
The antigens of eight strains of Strongyloides venezuelensis were identified by means of the indirect immunofluorescence antibody (IFAT), enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) tests. Infective larvae (L3) from these strains were obtained from Rattus norvegicus feces cultures. For IFAT, sections of L3 were used while the ELISA and IB, tests were conducted with alkaline extract. Ninety serum samples were tested: 30 from patients with S. stercoralis, 30 from patients with other parasitic diseases, and 30 from healthy subjects (free of parasites). Average sensitivity and specificity among all eight strains, both for IFAT and ELISA, were, respectively, 93% and 100%. In the IB, anti-S. stercoralis IgG recognized a single antigenic fraction with 45 kDa. Serum samples from patients with S. stercoralis revealed antigens from different strains of S. venezuelensis, indicating antigenic identity for possible use in the synthesis of recombinant antigen that could be useful in immunodiagnosis and vaccine against this parasite.
Machado
Eleuza Rodrigues
ER
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz s/n. CEP. 13083-970, Caixa Postal 6109, Campinas, SP, Brazil. ermachado@hotmail.com <ermachado@hotmail.com>
Faccioli
Lúcia Helena
LH
Costa-Cruz
Julia Maria
JM
Lourenço
Elaine Vicente
EV
Roque-Barreira
Maria Cristina
MC
Gonçalves-Pires
Maria do Rosário de Fátima
Mdo R
Ueta
Marlene Tiduko
MT
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007814:Larva / Q000145:classification / Q000276:immunology; D051381:Rats; D012377:Rodentia; D016205:Sigmodontinae; D013321:Strongyloides / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G