title: A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells.
abstract: Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication.
authors.affiliation: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana 59840, USA.
authors.forename: Diane C
authors.initials: DC
authors.lastname: Cockrell
authors.forename: Paul A
authors.initials: PA
authors.lastname: Beare
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Fischer
authors.forename: Dale
authors.initials: D
authors.lastname: Howe
authors.forename: Robert A
authors.initials: RA
authors.lastname: Heinzen
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002462:Cell Membrane / Q000187:drug effects; D002522:Chlorocebus aethiops; D016997:Coxiella burnetii / Q000166:cytology / Q000302:isolation & purification* / Q000648:ultrastructure; D004072:Digitonin / Q000378:metabolism*; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D014709:Vero Cells
chemical_list: D004072:Digitonin