Genome-wide identification of NBS resistance genes in Populus trichocarpa.
As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins ("NBS-LRR genes") play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of approximately 400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64 TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- and NBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in rust-infected and non-infected leaves using a whole-genome oligoarray. None showed an altered expression two days post inoculation.
Kohler
Annegret
A
Unité Mixte de Recherche INRA/UHP 1136 Interactions Arbres/Microorganismes, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, 54280 Champenoux, France. kohler@nancy.inra.fr
Rinaldi
Cécile
C
Duplessis
Sébastien
S
Baucher
Marie
M
Geelen
Danny
D
Duchaussoy
Frédéric
F
Meyers
Blake C
BC
Boerjan
Wout
W
Martin
Francis
F
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007113:Immunity, Innate / Q000235:genetics; D000090263:Leucine-Rich Repeat Proteins; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012275:Oryza / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics*; D011506:Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000235:genetics
D000090263:Leucine-Rich Repeat Proteins; D010940:Plant Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins