Adult olfactory bulbs from primates provide reliable ensheathing glia for cell therapy.
Olfactory bulb ensheathing glia (OB-OEG) from adult rodents promote functional and morphological repair after grafting into injured spinal cords. To provide insight into the feasibility of using OB-OEG in human therapy, we studied OB-OEG in primates to determine their suitability for spinal cord transplantation. Here, we show that OEG can be obtained from olfactory bulbs of adult macaca mulatta and nemestrina monkeys and compare their characteristics to those obtained from rats. In contrast to rodent OB-OEG, primate OB-OEG are nonsenescent, exhibit a longer lifespan, are less sensitive to high oxygen culture environment, and maintain a phenotype suitable for grafting for up to 2.5 months in vitro. Three-week cultures (short term) derived from a single macaca olfactory bulb provide enough OEG for autologous transplantation at the acute stage of injury, and after long-term cultures (2.5 months) may yield an additional 20 billion. OEG can be frozen for later use. Therefore, primate adult olfactory bulbs constitute a reliable source of OEG for cell therapy, and successful culture of these cells make autologous transplantation feasible.
Rubio
Mari-Paz
MP
Laboratory of Neural Regeneration, Institute of Biomedicine of Valencia, Spanish Council for Scientific Research (CSIC), Valencia, Spain.
Muñoz-Quiles
Cintia
C
Ramón-Cueto
Almudena
A
D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured / Q000502:physiology*; D005260:Female; D007797:Laminin / Q000378:metabolism; D008253:Macaca mulatta; D008254:Macaca nemestrina; D008297:Male; D009414:Nerve Growth Factors / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D019081:O Antigens / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology*; D010641:Phenotype; D011323:Primates / Q000033:anatomy & histology*; D051381:Rats; D017208:Rats, Wistar; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013997:Time Factors
D007797:Laminin; D009414:Nerve Growth Factors; D019081:O Antigens; D020800:Receptor, Nerve Growth Factor