Optical microscopy-based migration assay for human neutrophils.
This unit describes an in vitro microscopy assay for examining the migration of human neutrophils in two dimensions to identify the underlying cause of a migration defect and to evaluate a variety of migration parameters that cannot be studied using migration through a porous filter. Freshly isolated human neutrophils a placed in the chamber, stimulated, and images are collected at various time points. The data can be used to determine the effects of a pharmacological treatment on the migration of individual cells.
Pierini
Lynda M
LM
Weill Medical College of Cornell University, New York, New York, USA.
Maxfield
Frederick R
FR
D054443:Cell Migration Assays / Q000379:methods*; D006801:Humans; D008857:Microscopy, Interference; D009504:Neutrophils / Q000276:immunology*