title: Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa).
abstract: The genes insulin-like growth factor 2 (IGF2) and H19 express paternally and maternally, respectively, in humans, mice, sheep, and cattle. Additionally, IGF2 has been shown to be regulated by at least four promoters in a tissue- or development-specific manner. In the domestic pigs, the promoter- and tissue-specific imprinting pattern of IGF2 has not been well characterized, nor is the imprinting pattern of H19. In the present study, we identified two polymorphisms in each of IGF2 (exons 2 and 9) and H19 (exons 1 and 5) and determined the imprinting status of these two genes in 13 organs / tissues of week-old pigs. IGF2 P1 transcript is bi-allelically expressed (not imprinted) in all major organs studied, while the majority of IGF2 transcripts are expressed from promoters 2-4 and are imprinted. H19 is exclusively expressed from the maternal allele in all major organs, concurrent with observations in other species.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Bin
authors.forename: Carol
authors.initials: C
authors.lastname: Curchoe
authors.forename: Lan
authors.initials: L
authors.lastname: Yang
authors.forename: Dingyuan
authors.initials: D
authors.lastname: Feng
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Jiang
authors.forename: Michael
authors.initials: M
authors.lastname: O'Neill
authors.forename: X Cindy
authors.initials: XC
authors.lastname: Tian
authors.forename: Shouquan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016678:Genome; D018392:Genomic Imprinting; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated / Q000235:genetics*; D012661:Semen / Q000166:cytology / Q000502:physiology; D012867:Skin / Q000166:cytology; D013552:Swine / Q000235:genetics*
chemical_list: C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D007335:Insulin-Like Growth Factor II