[A simple, rapid and high-resolution banding method in polyacrylamide gels].
Although high-resolution picture can be obtained using the conventional banding method of DNA in PAGE gel, this method has complicated steps and will take very long time. In this paper, we reported an improved banding method of DNA in PAGE gel. DNA development in PAGE gel and comparison were performed by using two methods, the conventional banding method and the improved banding method, respectively. And the results indicated that the improved method has higher contrast between DNA band and the background in PAGE gel. So this improved method has higher resolution than the conventional one. Compared with the conventional banding method, this improved method has fewer steps, less time took and fewer reagents used. This improved method has replaced the conventional banding method completely in our laboratory.
Zhang
Yu-Shan
YS
Univesity of Electronic Science and Technology of China Zhongshan Institude, Guangdong, Zhongshan 528402, China. class2007ok@163.com
Bai
Xu-Feng
XF
D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000592:standards; D011110:Polymorphism, Genetic; D012680:Sensitivity and Specificity; D013997:Time Factors
D017931:DNA Primers; D004247:DNA