Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis.
Fingerprinting analysis of fresh ginseng according to root age was performed using 1H-NMR spectroscopy and multivariate analysis techniques. Various peaks were detected in the aliphatic (0-3 ppm), sugar (3-6 ppm), and aromatic (6-9 ppm) regions of the 1H-NMR spectra of the water extracts of fresh ginseng root. The use of principal components (PCs) analysis (PCA) for metabolomic profiling allowed the large 1H-NMR data set obtained for various metabolites to be reduced to PC1, PC2, and PC3. Two dimensional score plots showed clear separations with these three components at different roots ages, and explained 89.6% of the total variance. Canonical discriminant analysis identified the ginseng roots at various ages from the NMR results with over 89.9% discrimination accuracy. These results indicate that the combination of 1H-NMR and PCA provides a very promising tool for the authentication and quality control of fresh ginseng roots at different ages.
Shin
Yoo-Soo
YS
National Institute of Crop Science, RDA, Suwon 441-857, Korea.
Bang
Kyong-Hwan
KH
In
Dong-Soo
DS
Kim
Ok-Tae
OT
Hyun
Dong-Yoon
DY
Ahn
In-Ok
IO
Ku
Bon Cho
BC
Kim
Suk-Weon
SW
Seong
Nak-Sul
NS
Cha
Seon-Woo
SW
Lee
Dongho
D
Choi
Hyung-Kyoon
HK
D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D025341:Principal Component Analysis / Q000379:methods*