title: Human embryonic stem cell derivation from poor-quality embryos.
abstract: During in vitro fertilization, embryos deemed clinically useless based on poor morphology are typically discarded. Here we demonstrate a statistical correlation between the developmental stage of such poor-quality embryos and the yield of human embryonic stem (hES) cell lines. Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells.
authors.affiliation: Division of Newborn Medicine, Brigham & Women's Hospital, Boston, Massachusetts 02115, USA.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Lerou
authors.forename: Akiko
authors.initials: A
authors.lastname: Yabuuchi
authors.forename: Hongguang
authors.initials: H
authors.lastname: Huo
authors.forename: Ayumu
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Jessica
authors.initials: J
authors.lastname: Shea
authors.forename: Tina
authors.initials: T
authors.lastname: Cimini
authors.forename: Tan A
authors.initials: TA
authors.lastname: Ince
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ginsburg
authors.forename: Catherine
authors.initials: C
authors.lastname: Racowsky
authors.forename: George Q
authors.initials: GQ
authors.lastname: Daley
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006801:Humans