Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated.
We investigated the presence of glycoproteins in Borrelia burgdorferi. We did not find any evidence for glycosylation of the major outer membrane proteins OspA and OspB or the structural flagellar proteins FlaB and FlaA. We suggest that glycoproteins present on the surface of B. burgdorferi may be tightly bound culture medium glycoproteins.
Sterba
Ján
J
Biology Center of Academy of Sciences of the Czech Republic, Institute of Parasitology, Branisovská 31, CZ-37005 Ceské Budejovice, Czech Republic. sterbaj@paru.cas.cz
Vancová
Marie
M
Rudenko
Nataliia
N
Golovchenko
Maryna
M
Tremblay
Tammy-Lynn
TL
Kelly
John F
JF
MacKenzie
C Roger
CR
Logan
Susan M
SM
Grubhoffer
Libor
L
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001428:Bacterial Vaccines / Q000235:genetics / Q000378:metabolism; D025065:Borrelia burgdorferi / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D004591:Electrophoresis, Polyacrylamide Gel; D005408:Flagellin / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D021241:Spectrometry, Mass, Electrospray Ionization
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; C072045:OspA protein; D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin; C077030:OspB protein, Borrelia burgdorferi