title: Development and validation of a LC method for determination of genistein in topical nanoemulsions.
abstract: The aim of this study was to develop and validate an isocratic LC method for the quantification of genistein in topical nanoemulsions. The analyses were performed at room temperature on a reversed-phase C18 column using a mobile phase composed of methanol/water/acetonitrile (70:25:5, w/w/w) at 1.0 ml x min(-1). The detection was carried out on a UV detector at 327 nm. The linearity, in the range of 25-75 microg/ml, presented a determination coefficient (r2) higher than 0.999, calculated by the least square method. No interferences from the excipients (egg-lecithin, octyldodecanol or medium chain triglycerides) were detected. The R.S.D. values for intra- and inter-day precision experiments were lower than 2.3%. The recovery of genistein from nanoemulsions ranged from 96.6% to 106.6%. The excellent performance of the method, its linearity, accuracy and precision, demonstrate that it can be readily used to quantify genistein incorporated in nanoemulsions.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Cappra Silva
authors.forename: L S
authors.initials: LS
authors.lastname: Koester
authors.forename: P
authors.initials: P
authors.lastname: Mayorga
authors.forename: V
authors.initials: V
authors.lastname: Linck Bassani
authors.forename: H
authors.initials: H
authors.lastname: Teixeira
mesh_terms: D000287:Administration, Topical; D016588:Anticarcinogenic Agents / Q000008:administration & dosage* / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002853:Chromatography, Liquid; D004655:Emulsions; D005079:Excipients; D019833:Genistein / Q000008:administration & dosage* / Q000032:analysis*; D010316:Particle Size; D015203:Reproducibility of Results
chemical_list: D016588:Anticarcinogenic Agents; D004655:Emulsions; D005079:Excipients; D019833:Genistein