Matrix metalloproteinases.
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type I collagen fibrils, direct and reverse zymography, enzyme capture based on a-2 macroglubulin and TIMP-1 and -2, and demonstration of crytic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.
Birkedal-Hansen
Henning
H
National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, USA.
Yamada
Susan
S
Windsor
Jack
J
Poulsen
Anne Havemose
AH
Lyons
Guy
G
Stetler-Stevenson
William
W
Birkedal-Hansen
Bente
B
D000818:Animals; D002364:Caseins / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004792:Enzyme Precursors / Q000737:chemistry / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000737:chemistry* / Q000302:isolation & purification*; D008722:Methods; D051381:Rats; D013379:Substrate Specificity; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000737:chemistry*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000737:chemistry*; D000511:alpha-Macroglobulins / Q000737:chemistry*
D002364:Caseins; D024042:Collagen Type I; D004792:Enzyme Precursors; D005456:Fluorescent Dyes; D061965:Matrix Metalloproteinase Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D000511:alpha-Macroglobulins; D019716:Tissue Inhibitor of Metalloproteinase-2; D005780:Gelatin; D020782:Matrix Metalloproteinases; D003545:Cysteine