title: [Cloning and analyzing of rice blast resistance gene Pi-ta+ allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan].
abstract: A 4,672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast resistance gene Pi-ta+ has been cloned from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan by polymerase chain reaction method. The coding region shares 99.86% and 98.78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively. There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta+ gene,compared with Pi-ta from Yashiro-mochi. Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele, because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA. Pi-ta+ allele can cause disease resistance response to rice blast pathogens in plant cells. Differences in DNA sequence, deduced amino acid sequence and antibacterial spectrum may make the Pi-ta+ allele new resistant characteristics. Finding and cloning of Pi-ta+ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources.
authors.affiliation: School of Life Science, Yunnan University, Kunming 650091, China.
authors.forename: Xian-Sheng
authors.initials: XS
authors.lastname: Geng
authors.forename: Ming-Zhi
authors.initials: MZ
authors.lastname: Yang
authors.forename: Xing-Qi
authors.initials: XQ
authors.lastname: Huang
authors.forename: Zai-Quan
authors.initials: ZQ
authors.lastname: Cheng
authors.forename: Jian
authors.initials: J
authors.lastname: Fu
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics*; D007438:Introns / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques; D012275:Oryza / Q000235:genetics* / Q000502:physiology*; D010935:Plant Diseases; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D020506:Untranslated Regions / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D020506:Untranslated Regions