title: C242T polymorphism of NADPH oxidase p22phox and recurrence of cardiovascular events in coronary artery disease.
abstract: OBJECTIVE
  The common C242T polymorphism in the gene for the p22phox subunit of NADPH oxidase has been reported to be negatively associated with oxidative stress, but whether it confers prognostic information is not yet clear.
  
  
  RESULTS
  The incidence of major adverse cardiovascular events (MACE) were determined in 237 patients with coronary stenosis during a median follow-up of 7.8 years. The p22phox genotypes were evaluated in 213 patients (89.9%) by polymerase chain reaction and RsaI. digestion. Plasma levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker of oxidative stress, were also measured. In the univariate analysis, patients with CT/TT genotypes showed reduced recurrence of cardiovascular deaths, nonfatal MI, and revascularization procedures compared with homozygous carriers of the C allele. After controlling for confounders, a significantly lower risk of new revascularization procedures (HR=0.31, 95% CI 0.12 to 0.70; P=0.014) remained associated with the T allele. The Kaplan-Meier analysis showed a longer survival free from fatal and nonfatal MI in carriers of T allele (P<0.001). The presence of the 242T allele was associated with significantly reduced plasma concentrations of 8-OHdG.
  
  
  CONCLUSIONS
  The 242T allele was a predictor of lower risk of recurrence of cardiovascular events in high-risk patients and was associated with reduced systemic oxidative stress.
authors.affiliation: Department of Clinical and Medical Therapy, Unit of Medical Therapy, La Sapienza University, Rome, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Arca
authors.forename: Beatrice
authors.initials: B
authors.lastname: Conti
authors.forename: Anna
authors.initials: A
authors.lastname: Montali
authors.forename: Pasquale
authors.initials: P
authors.lastname: Pignatelli
authors.forename: Filomena
authors.initials: F
authors.lastname: Campagna
authors.forename: Francesco
authors.initials: F
authors.lastname: Barillà
authors.forename: Gaetano
authors.initials: G
authors.lastname: Tanzilli
authors.forename: Roberto
authors.initials: R
authors.lastname: Verna
authors.forename: Annarita
authors.initials: A
authors.lastname: Vestri
authors.forename: Carlo
authors.initials: C
authors.lastname: Gaudio
authors.forename: Francesco
authors.initials: F
authors.lastname: Violi
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000483:Alleles; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000201:enzymology* / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012008:Recurrence; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D019255:NADPH Oxidases; C086986:CYBA protein, human; D003849:Deoxyguanosine