title: Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17.
abstract: A mixture of two structurally distinct neutral O-polysaccharides was obtained by mild acid degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense S17. The following structures of the O-polysaccharides were established by composition and methylation analyses, Smith degradation, and 1H and 13C NMR spectroscopy, including a 2D NOESY experiment: [Formula: see text] where L-Rha2Me stands for 2-O-methyl-L-rhamnose and SHb for the (S)-3-hydroxybutanoyl group. The occurrence of two distinct polysaccharides is reported for the first time in Azospirillum spp.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.
authors.forename: Yuliya P
authors.initials: YP
authors.lastname: Fedonenko
authors.forename: Olga N
authors.initials: ON
authors.lastname: Konnova
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Konnova
authors.forename: George V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Ignatov
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D015806:Azospirillum brasilense / Q000737:chemistry*; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
chemical_list: D008070:Lipopolysaccharides