title: Multilaboratory testing of antifungal combinations against a quality control isolate of Candida krusei.
abstract: Candida krusei ATCC 6258 was tested by eight laboratories using 96-well plates containing checkerboard pairwise combinations of amphotericin B (AMB), posaconazole (PSC), caspofungin (CSP), and voriconazole (VRC). The methodology led to reproducible results across the laboratories. All drug combinations yielded MICs lower than the MICs of any two drugs tested singly, and combinations of AMB, PSC, CSP, and VRC were indifferent (no antagonism) by summations of fractional inhibitory concentration.
authors.affiliation: Mycology Laboratory, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208, USA. vishnu@wadsworth.org
authors.forename: Vishnu
authors.initials: V
authors.lastname: Chaturvedi
authors.forename: Rama
authors.initials: R
authors.lastname: Ramani
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Ghannoum
authors.forename: Scott B
authors.initials: SB
authors.lastname: Killian
authors.forename: Nicole
authors.initials: N
authors.lastname: Holliday
authors.forename: Cindy
authors.initials: C
authors.lastname: Knapp
authors.forename: Luis
authors.initials: L
authors.lastname: Ostrosky-Zeichner
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Messer
authors.forename: Michael A
authors.initials: MA
authors.lastname: Pfaller
authors.forename: Naureen J
authors.initials: NJ
authors.lastname: Iqbal
authors.forename: Beth A
authors.initials: BA
authors.lastname: Arthington-Skaggs
authors.forename: Jose A
authors.initials: JA
authors.lastname: Vazquez
authors.forename: Tin
authors.initials: T
authors.lastname: Sein
authors.forename: John H
authors.initials: JH
authors.lastname: Rex
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Walsh
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D000077336:Caspofungin; D004359:Drug Therapy, Combination; D054714:Echinocandins / Q000494:pharmacology; D006801:Humans; D007753:Laboratories; D055666:Lipopeptides; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D011743:Pyrimidines / Q000494:pharmacology; D011786:Quality Control; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D011743:Pyrimidines; D014230:Triazoles; C101425:posaconazole; D000666:Amphotericin B; D000077336:Caspofungin; D065819:Voriconazole