Sesquiterpene quinones and related metabolites from Phyllosticta spinarum, a fungal strain endophytic in Platycladus orientalis of the Sonoran Desert.
Five new metabolites, (+)-(5 S,10 S)-4'-hydroxymethylcyclozonarone ( 1), 3-ketotauranin ( 3), 3alpha-hydroxytauranin ( 4), 12-hydroxytauranin ( 5), and phyllospinarone ( 6), together with tauranin ( 2), were isolated from Phyllosticta spinarum, a fungal strain endophytic in Platycladus orientalis. The structures of the new compounds were determined on the basis of their 1D and 2D NMR spectroscopic data and chemical interconversions. All compounds were evaluated for inhibition of cell proliferation in a panel of five cancer cell lines, and only tauranin ( 2) showed activity. When tested in a flow cytometry-based assay, tauranin induced apoptosis in PC-3M and NIH 3T3 cell lines.
Wijeratne
E M Kithsiri
EM
SW Center for Natural Products Research and Commercialization, Office of Arid Lands Studies, College of Agriculture and Life Sciences, University of Arizona, 250 E. Valencia Road, Tucson, Arizona 85706-6800, USA.
Paranagama
Priyani A
PA
Marron
Marilyn T
MT
Gunatilaka
Malkanthi K
MK
Arnold
A Elizabeth
AE
Gunatilaka
A A Leslie
AA
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001203:Ascomycota / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D029779:Cupressaceae / Q000382:microbiology; D003889:Desert Climate; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011809:Quinones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D000970:Antineoplastic Agents; D011809:Quinones; D012717:Sesquiterpenes; C528158:tauranin