Infrared laser light further improves bone healing when associated with bone morphogenic proteins: an in vivo study in a rodent model.
OBJECTIVE
This study assessed histologically the effect of laser photobiomodulation (LPBM) on the repair of surgical defects created in the femurs of Wistar rats treated or not treated with bone morphogenic proteins (BMPs) and organic bovine bone graft.


BACKGROUND
This paper is part of an ongoing series of works in which biomaterials are used in association with LPBM. Several previous reports by our group have shown that the use of laser photobiomodulation improves the treatment of bone defects.


METHODS
Forty-eight adult male Wistar rats were divided into four randomized groups: group I (control, n = 12); group II (LPBM, n = 12); group III (BMPs + organic bovine bone graft, n = 12); and group IV (BMPs + organic bovine bone graft + LPBM, n = 12). The irradiated groups received seven irradiations every 48 h, beginning immediately after the surgical procedure. The laser therapy (lambda = 830 nm, 40 mW CW, varphi = 0.6 mm) consisted of 16 J/cm(2) per session divided equally over four points (4 J/cm(2) each) around the defect. The subjects were sacrificed after 15, 21, and 30 d, and the specimens were routinely embedded in wax, stained with hematoxylin and eosin and sirius red, and analyzed under light microscopy.


RESULTS
The results showed histological evidence of increased deposition of collagen fibers (at 15 and 21 d), as well as an increased amount of well-organized bone trabeculae at the end of the experimental period (30 d) in the irradiated animals versus the non-irradiated controls.


CONCLUSIONS
The use of LPBM with BMPs and organic bovine bone grafts increases the positive biomodulating effects of laser light.
Gerbi
Marleny E Martinez
ME
School of Dentistry, Federal University of Pernambuco, Recife, PE, Salvador.
Marques
Aparecida M C
AM
Ramalho
Luciana Maria Pedreira
LM
Ponzi
Elizabeth Arruda Carneiro
EA
Carvalho
Carolina Montagn
CM
Santos
Rafael De Carneiro
Rde C
Oliveira
Priscila Chagas
PC
Nóia
Manuela
M
Pinheiro
Antonio Luiz B
AL
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage*; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects* / Q000528:radiation effects*; D005269:Femur / Q000187:drug effects / Q000503:physiopathology / Q000528:radiation effects; D007259:Infrared Rays / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins