title: Dynamic epigenetic states of ribosomal RNA promoters during the cell cycle.
abstract: It has previously been shown that the ribosomal RNA (rRNA) promoter is regulated through epigenetic mechanisms. It is unclear however whether epigenetic marks are stable in somatic cells or whether and how they vary with cell cycle dynamics. Here we present an analysis of epigenetic marks in cells positioned at different phases of the cell cycle following synchronization using a double thymidine block. We show that the levels of acetylated histone 4 are highest in early S phase, coinciding with the peak of binding of the transcriptional activators UBF and MBD3 to the rRNA promoter. Additionally, binding of the DNA methyltransferase DNMT1 is highest during mid-S phase, while DNMT3B binding peaks later in G2. Bisulfite mapping of the rRNA promoter reveals that the DNA methylation state varies during the cell cycle being lowest during early and late S phase. Interestingly, although the interaction of RNA polymerase I with the promoter and its progress along the gene coincides with epigenetic activation, the burst in levels of rRNA transcript did not occur until after DNA synthesis was complete. This suggests that although the rRNA promoter is poised for transcription early in the cell cycle, the accumulation of rRNA transcripts requires additional signals later in the cell cycle. This data is consistent with the idea that epigenetic states are dynamic in somatic cells and might participate in physiological cellular responses.
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, 3655 Sir William Osler Promenade, Montréal, Québec H3G 1Y6, Canada.
authors.forename: Shelley E
authors.initials: SE
authors.lastname: Brown
authors.forename: Moshe
authors.initials: M
authors.lastname: Szyf
mesh_terms: D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D019175:DNA Methylation; D044127:Epigenesis, Genetic / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology*; D012335:RNA, Ribosomal / Q000235:genetics / Q000502:physiology*
chemical_list: D012335:RNA, Ribosomal