[Indication of extremely dangerous infectious pathogens using immunochromatography and digital video analysis].
The use of immunochromatographic indicatory elements based on antibody conjugates and colloidal gold was suggested to detect cells and the antigens of extremely dangerous infectious pathogens. The specificity and specific activity (sensitivity) of the mentioned elements were studied on vaccinal strains of plague, anthrax, and tularemia pathogens. The researchers studied a possibility to increase the sensitivity of immunochromatographic analysis using computed scanning and Reflecom, a specialized digital video recorder of immunochromatogramms. The study demonstrated that the use of electronic devices to record immunochromatogramms increased the sensitivity to microbial cells and antigens 1.5 to 2 times vs. visual registration and simplified documentation of the results.
Iarkov
S P
SP
Tret'iakov
S I
SI
Basharova
L A
LA
Zlobin
V N
VN
D000881:Anthrax / Q000175:diagnosis / Q000382:microbiology; D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000276:immunology*; D002845:Chromatography / Q000295:instrumentation*; D004867:Equipment Design; D005604:Francisella tularensis / Q000276:immunology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D010930:Plague / Q000175:diagnosis / Q000382:microbiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014406:Tularemia / Q000175:diagnosis / Q000382:microbiology; D014741:Video Recording / Q000295:instrumentation*; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial