Intensified biochip system using chemiluminescence for the detection of Bacillus globigii spores.
This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact intensified biochip detection system. The enzymatic amplification was found to be an attractive method for detection of low spore concentrations when combined with the intensified biochip device. This system was capable of detecting approximately 1 x 10(5) Bacillus globigii spores. Moreover, the chemiluminescence method, combined with the self-contained biochip design, allows for a simple, compact system that does not require laser excitation and is readily adaptable to field use.
Stratis-Cullum
Dimitra N
DN
US Army Research Laboratory, AMSRD-ARL-SE-EO, 2800 Powder Mill Road, Adelphi, MD, 20783, USA. dimitra.stratiscullum1@us.army.mil
Griffin
Guy D
GD
Mobley
Joel
J
Vo-Dinh
Tuan
T
D001407:Bacillus / Q000254:growth & development / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D046208:Microchip Analytical Procedures / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000254:growth & development / Q000302:isolation & purification*