title: Lentivirus transduction of bone marrow hemopoietic precursor cells with Lin-c-kit+ phenotype ex vivo using a genetic construct containing green fluorescent protein gene.
abstract: We developed a technology of labeling bone marrow precursor cells with the Lin-c-kit+ phenotype in culture by green fluorescent protein gene using a lentivirus vector. The proposed system provides effective transduction of bone marrow precursor cells and high transgene expression level in vitro (27%). The integration of the transgene into the transduced cell genome in vivo was verified by the method of splenic colonies.
authors.affiliation: Department of Cell Biology, Institute of Experimental Cardiology, Cardiology Research-and-Production Complex, Federal Agency for Health Care and Social Development, Moscow. cardiocell@cardio.ru
authors.forename: N V
authors.initials: NV
authors.lastname: Radyukhina
authors.forename: P N
authors.initials: PN
authors.lastname: Rutkevich
authors.forename: T I
authors.initials: TI
authors.lastname: Aref'yeva
authors.forename: T Kh
authors.initials: TKh
authors.lastname: Gurskaya
authors.forename: I N
authors.initials: IN
authors.lastname: Rybalkin
authors.forename: A Ya
authors.initials: AY
authors.lastname: Shevelyov
authors.forename: M A
authors.initials: MA
authors.lastname: Slinkin
authors.forename: T N
authors.initials: TN
authors.lastname: Vlasik
authors.forename: O P
authors.initials: OP
authors.lastname: Il'yinskaya
authors.forename: E M
authors.initials: EM
authors.lastname: Tararak
mesh_terms: D000818:Animals; D005260:Female; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000821:virology*; D016086:Lentivirus / Q000502:physiology*; D008297:Male; D051379:Mice; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins