title: Matrix metalloproteinases.
abstract: Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type I collagen fibrils, direct and reverse zymography, enzyme capture based on a-2 macroglubulin and TIMP-1 and -2, and demonstration of crytic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.
authors.affiliation: National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Henning
authors.initials: H
authors.lastname: Birkedal-Hansen
authors.forename: Susan
authors.initials: S
authors.lastname: Yamada
authors.forename: Jack
authors.initials: J
authors.lastname: Windsor
authors.forename: Anne Havemose
authors.initials: AH
authors.lastname: Poulsen
authors.forename: Guy
authors.initials: G
authors.lastname: Lyons
authors.forename: William
authors.initials: W
authors.lastname: Stetler-Stevenson
authors.forename: Bente
authors.initials: B
authors.lastname: Birkedal-Hansen
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004792:Enzyme Precursors / Q000737:chemistry / Q000302:isolation & purification; D005456:Fluorescent Dyes / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000737:chemistry* / Q000302:isolation & purification*; D008722:Methods; D051381:Rats; D013379:Substrate Specificity; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000737:chemistry*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000737:chemistry*; D000511:alpha-Macroglobulins / Q000737:chemistry*
chemical_list: D002364:Caseins; D024042:Collagen Type I; D004792:Enzyme Precursors; D005456:Fluorescent Dyes; D061965:Matrix Metalloproteinase Inhibitors; D019715:Tissue Inhibitor of Metalloproteinase-1; D000511:alpha-Macroglobulins; D019716:Tissue Inhibitor of Metalloproteinase-2; D005780:Gelatin; D020782:Matrix Metalloproteinases; D003545:Cysteine