Effective penetration of cell-permeable peptide mimic of tyrosine residue 654 domain of beta-catenin into human renal tubular epithelial cells.
Phosphorylation of beta-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition (EMT). Introducing mimic peptide of tyrosine residue 654 domain of beta-catenin into cells may influence phosphorylation of beta-catenin tyrosine residue 654. To deliver this mimic peptide into renal epithelial cells, we used penetratin as a vector, which is a novel cell permeable peptide, to deliver hydrophilic molecules into cells. A tyrosine 654 residue domain mimic peptide of beta-catenin (PM) with fused penetratin was constructed, purified and then detected for the penetration of the mimic peptide into human renal tubular epithelial cells (HK-2). The results showed that purified fusion mimic peptide could efficiently and rapidly translocate into human renal tubular epithelial cells. It is concluded that a cell-permeable peptides mimic of tyrosine residue 654 domain of beta-catenin was successfully obtained, which may provide a useful reagent for interfering the human renal tubular epithelial-mesenchymal transition.
Zeng
Rui
R
Division of Nephrology, Department of Internal Medicine, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, 430030, China.
Xu
Gang
G
Han
Min
M
D002352:Carrier Proteins / Q000378:metabolism*; D057846:Cell-Penetrating Peptides; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology*; D010455:Peptides / Q000378:metabolism; D010539:Permeability; D010766:Phosphorylation; D014443:Tyrosine / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D010455:Peptides; D051176:beta Catenin; D014443:Tyrosine; C414409:penetratin