[Fabrication of alginate microsphere for controlled release and investigation of its release characteristics in vitro].
This study sought to producte alginate sodium microsphere for controlled release bovine serum albumin(BSA) and to investigate the protein release profile of the BSA-alginate sodium microsphere in vitro, which threw some light on the angiogenesis of tissue engineering bone with vascular endothelial growth factor (VEGF) controlled release under stress. The BSA-alginate sodium microsphere was fabricated with W/O emulsification and ion cross-linking method using alginate sodium. The appearance, microsphere diameter and envelopment rate were detected, and the release characteristics of the BSA-alginate sodium microsphere in vitro was investigated. The alginate microsphere was found to be spherical in shape and evenly distributed. Its mean grain diameter was determined to be 230 +/- 60 microm, carrying capacity 80.3 microg/mg and envelopment rate 61%. Smooth controlled release in BSA-alginate sodium microsphere was shown to last more than 2 weeks. Alginate sodium proved an excellent biodegradable material for protein or polypeptide controlled release. The emulsification and ion cross-linking method was noted to be simple; it was propitious to the structural and functional stablility of protein or polypeptide, thus leading to the prolonged efficacious time of the microsphere.
He
Qingyi
Q
Department of Orthopedic, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. hqy1225@yahoo.com.cn
Wen
Lisheng
L
Li
Qiang
Q
Xu
Jianzhong
J
Luo
Fei
F
D000464:Alginates / Q000008:administration & dosage* / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003692:Delayed-Action Preparations / Q000138:chemical synthesis*; D004337:Drug Carriers; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry; D008863:Microspheres; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*
D000464:Alginates; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006603:Hexuronic Acids; D012710:Serum Albumin, Bovine; D020723:Glucuronic Acid