title: Design and construction of a preparative-scale dynamic field gradient focusing apparatus.
abstract: A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, approximately O (10 mgs). This paper explains how the preparative-scale DFGF apparatus was designed and fabricated. Scaled-down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative-scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein.
authors.affiliation: School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
authors.forename: Noah I
authors.initials: NI
authors.lastname: Tracy
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.forename: Cornelius F
authors.initials: CF
authors.lastname: Ivory
mesh_terms: D002021:Buffers; D003201:Computers; D003625:Data Collection; D003627:Data Interpretation, Statistical; D004566:Electrodes; D004581:Electronics; D004867:Equipment Design; D006454:Hemoglobins / Q000302:isolation & purification; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000295:instrumentation*; D016014:Linear Models; D008567:Membranes, Artificial
chemical_list: D002021:Buffers; D006454:Hemoglobins; D007202:Indicators and Reagents; D008567:Membranes, Artificial