Transfection of cultured eukaryotic cells using cationic lipid reagents.
The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. This unit describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using cationic lipid reagents.
Hawley-Nelson
Pamela
P
Centocor, Inc., Radnor, Pennsylvania, USA.
Ciccarone
Valentina
V
Moore
Meredith L
ML
D000818:Animals; D016367:Baculoviridae; D002412:Cations / Q000737:chemistry; D002448:Cell Adhesion; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D005057:Eukaryotic Cells / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D007313:Insecta / Q000166:cytology; D019169:Jurkat Cells; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D002412:Cations; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering; D004247:DNA