dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.
ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase II to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.
Murawska
Magdalena
M
Institut für Molekularbiologie und Tumorforschung, University of Marburg, 35033 Marburg, Germany.
Kunert
Natascha
N
van Vugt
Joke
J
Längst
Gernot
G
Kremmer
Elisabeth
E
Logie
Colin
C
Brehm
Alexander
A
D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D002875:Chromosomes / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000201:enzymology; D010802:Phylogeny; D011485:Protein Binding; D014158:Transcription, Genetic / Q000235:genetics*
D001324:Autoantigens; D029721:Drosophila Proteins; D006657:Histones; C116095:Mi-2 protein, Drosophila; D009707:Nucleosomes; D000255:Adenosine Triphosphate; D004247:DNA; D000251:Adenosine Triphosphatases; C528126:CHD3 protein, Drosophila; D004265:DNA Helicases