title: Application of polymerase chain reaction-based assays for rapid identification and antibiotic resistance screening of Helicobacter pylori in gastric biopsies.
abstract: The benefits of using a multiplex detection polymerase chain reaction (PCR) assay for Helicobacter pylori speciation and 2 real-time probe hybridization assays determining clarithromycin and tetracycline susceptibilities in gastric biopsies from 171 dyspeptic patients were investigated. Overall, 70 of 71 H. pylori culture-positive biopsies were PCR positive. For the 100 culture-negative biopsies, PCR identified a further 29 H. pylori positives (17% overall) and presence of resistance markers for clarithromycin (20/28) and tetracycline (2/28). The results demonstrated that PCR testing was valuable in providing improved detection rates and antibiotic susceptibility information when H. pylori culture was unsuccessful.
authors.affiliation: Gastrointestinal, Emerging, and Zoonotic Infections Department, Centre for Infections, Health Protection Agency, London NW9 5HT, UK.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Chisholm
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Owen
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001706:Biopsy; D017291:Clarithromycin / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004415:Dyspepsia / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D013270:Stomach / Q000473:pathology; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013752:Tetracycline; D017291:Clarithromycin