The effect of gelatin incorporation into electrospun poly(L-lactide-co-epsilon-caprolactone) fibers on mechanical properties and cytocompatibility.
Very elastic poly(L-lactide-co-epsilon-caprolactone) (PLCL) (50:50) copolymer blended with gelatin was electrospun into microfibers from a hexafluoroisopropanol solution. PLCL fiber sheet exhibited the unique soft and flexible behavior while gelatin fiber was hard and brittle. As the gelatin content of PLCL/gelatin fibers increased, Young's modulus was increased, but the elongation was decreased compared to those of PLCL. However, fibers containing 10-30 wt% gelatin demonstrated an enhanced tensile strength with still high elongation to be beneficial for tissue engineering scaffolds. The cytocompatibility of electrospun fiber sheets was evaluated by fibroblasts (NIH-3T3) cell culture. The initial cell adhesion on various fibers after 5h was somewhat similar, but in the order of PLCL>PLCL70/gelatin30 approximately PLCL50/gelatin50>PLCL90/gelatin10 approximately gelatin>PLCL30/gelatin70. However, the cell proliferation exhibited a completely different and strong dependence on the fiber composition: a very high proliferation rate on PLCL90/gelatin10, followed by PLCL>gelatin>PLCL70/gelatin30. Such an enhanced effect of gelatin, especially at 10 wt% content, on strength and cytocompatibility of PLCL/gelatin fibers would be very preferable for tissue engineering scaffolds.
Lee
Jongman
J
Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea.
Tae
Giyoong
G
Kim
Young Ha
YH
Park
In Su
IS
Kim
Sang-Heon
SH
Kim
Soo Hyun
SH
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage* / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects*; D004548:Elasticity; D004563:Electrochemistry / Q000379:methods; D005780:Gelatin / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000008:administration & dosage* / Q000737:chemistry*; D016062:Porosity; D012399:Rotation; D013314:Stress, Mechanical; D013718:Tensile Strength
D001672:Biocompatible Materials; D011091:Polyesters; C062968:lactide-caprolactone copolymer; D005780:Gelatin