title: In vitro analysis of SV40 DNA replication.
abstract: This unit describes a method for studying cellular factors that affect eukaryotic replication by using a plasmid that contains an SV40 origin of replication and supplying the viral helicase T antigen. Replication factors are supplied by S100 extracts of 293 cells. The unit also contains protocols for expression and purification of T antigen and analysis of the products of replication.
authors.affiliation: Genetics Institute, Cambridge, Massachusetts, USA.
authors.forename: Y L
authors.initials: YL
authors.lastname: Lin
authors.forename: A
authors.initials: A
authors.lastname: Dutta
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D004261:DNA Replication / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000235:genetics
chemical_list: D000952:Antigens, Polyomavirus Transforming