Cyclodextrin production and genetic characterization of cyclodextrin glucanotranferase of Paenibacillus graminis.
Paenibacillus graminis strains were described recently as cyclodextrin (CD) producers. Cyclodextrins are produced by cyclodextrin glucanotransferase (CGTase) which has not been characterized in P. graminis. Similar amounts of alpha- and beta-CDs were produced by P. graminis (MC22.13) and P. macerans (LMD24.10(T)). Primers were designed to sequence the gene encoding CGTase from P. graminis. A phylogenetic tree was constructed and P. graminis CGTase protein showed to be closer (79.4% protein identity) to P. macerans |P31835|. Hybridization studies suggested that the gene encoding CGTase is located in different positions in the genomes of P. macerans and P. graminis.
Vollú
Renata Estebanez
RE
Laboratório de Genética Microbiana, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brasil.
da Mota
Fabio Faria
FF
Gomes
Eliane Aparecida
EA
Seldin
Lucy
L
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003505:Cyclodextrins / Q000096:biosynthesis*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics*; D016937:Gram-Positive Endospore-Forming Rods / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D001426:Bacterial Proteins; D003505:Cyclodextrins; D017931:DNA Primers; D004269:DNA, Bacterial; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase