title: Impact of lux gene insertion on bacterial surface properties and transport.
abstract: Genetic markers have been in popular use for tracing microbial movement in the environment. However, the impact of genetic marker insertion on microbial surface properties and consequent transport is often ignored. For this research, we investigated the impact of luminescence-based genetic marker insertion on bacterial surface properties and transport. Typical Gram-positive bacterial strains of Lactobacillus casei, Streptococcus mitis and Micrococcus luteus were used as model bacterial strains in this research. We manipulated gene transfer to observe the impact of lux gene insertion on bacterial surface properties based on contact angle measurements, and we conducted column experiments to evaluate the impact of lux gene insertion on bacterial transport. After lux gene insertion, bacterial interactions with the porous media increased, demonstrating stronger deposition potential in the porous media. Accordingly, retention of the daughter strains increased. Lux gene insertion also resulted in an increase in bacterial dispersion and equilibrium adsorption in the porous media. The bacterial deposition coefficient was found to correlate with the free energy of interactions between bacteria and the porous media.
authors.affiliation: Department of Civil and Environmental Engineering, FAMU-FSU College of Engineering, 2525 Pottsdamer Street, Tallahassee, FL 32310, USA. gchen@eng.fsu.edu
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Vijay Penagonda
authors.initials: VP
authors.lastname: Srinivasa Ranga
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Mao
authors.forename: Kevin
authors.initials: K
authors.lastname: Chen
authors.forename: Hanzi
authors.initials: H
authors.lastname: Qiao
mesh_terms: D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D019015:Geologic Sediments / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016982:Micrococcus luteus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008954:Models, Biological; D016254:Mutagenesis, Insertional; D034361:Streptococcus mitis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013816:Thermodynamics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C085427:LuxI protein, Bacteria; D014157:Transcription Factors