Statistical optimization of medium for the production of pyruvate oxidase by the recombinant Escherichia coli.
Pyruvate oxidase (PyOD) is a very useful enzyme for clinical diagnostic applications and environmental monitor. Optimization of the fermentation medium for maximization of PyOD constitutively, production by Escherichia coli DH5alpha/pSMLPyOD was carried out. Response surface methodology (RSM) was used to optimize the medium constituents. A 2(6-2) fractional factorial design (first order model) was carried out to identify the significant effect of medium components towards PyOD production. Statistical analysis of results shows that yeast extract, ammonium sulfate and composite phosphate were significant factors on PyOD production. The optimized values of these three factors were obtained by RSM based on the result of a 2(3) central composite rotatable design. Under these proposed optimized medium, the model predicted a PyOD activity of 610 U/L and via experimental rechecking the model, an activity of 670 U/L was attained.
Zhao
Jie
J
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237 Shanghai, People's Republic of China.
Wang
Yonghong
Y
Chu
Ju
J
Zhang
Siliang
S
Zhuang
Yingping
Y
Yuan
Zhongyi
Z
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005818:Genetic Engineering; D007778:Lactobacillus / Q000201:enzymology; D015233:Models, Statistical; D011771:Pyruvate Oxidase / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D011994:Recombinant Proteins; D011771:Pyruvate Oxidase