Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis.
Eighteen Salmonella isolates from both human and food (non-human) sources (fish, meat, and poultry) were characterized using conventional culture methods, biochemical, serological, and molecular analyses. REP-PCR and RAPD produced DNA profiles for differentiation purposes. Enterobacterial repetitive intergenic consensus (ERIC), repetitive extragenic palindronic (REP) and BOXAIR primers were selected for REP-PCR and two arbitrary primers, namely OPP-16 and OPS-11 were used for RAPD to generate DNA fingerprints from the Salmonella isolates. REP-PCR method showed greater discriminatory power in differentiating closely related strains of the related strains of Salmonella and produced more complex banding patterns as compared with RAPD. A dendogram was constructed with both sets of profiles using SPSS Version 13.0 computer software and showed that most human isolates were separately clustered from the non-human isolates. Two of the human isolates were closely related to some of the non-human isolates. A good correlation was also observed between the serogrouping of the O antigen and the molecular profiles obtained from REP-PCR and RAPD data of the Salmonella isolates. The results of a principal coordinate analysis (PCA) corresponded to the clustering in the dendrogram.
Albufera
U
U
Agricultural and Food Sciences Department, University of Mauritius, Reduit, Mauritius.
Bhugaloo-Vial
P
P
Issack
M I
MI
Jaufeerally-Fakim
Y
Y
D000818:Animals; D002417:Cattle; D002645:Chickens; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005516:Food Microbiology; D006801:Humans; D007223:Infant; D008297:Male; D008460:Meat / Q000382:microbiology; D010473:Perciformes; D019105:Random Amplified Polymorphic DNA Technique; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000382:microbiology*; D012703:Serotyping; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D004269:DNA, Bacterial