Runx1 is involved in primitive erythropoiesis in the mouse.
Targeted disruption of the Runx1/ AML1 gene in mice has demonstrated that it is required for the emergence of definitive hematopoietic cells but that it is not essential for the formation of primitive erythrocytes. These findings led to the conclusion that Runx1 is a stage-specific transcription factor acting only during definitive hematopoiesis. However, the zebrafish and Xenopus homologs of Runx1 have been shown to play roles in primitive hematopoiesis, suggesting that mouse Runx1 might also be involved in the development of primitive lineages. In this study, we show that primitive erythrocytes in Runx1(-/-) mice display abnormal morphology and reduced expression of Ter119, Erythroid Kruppel-like factor (EKLF, KLF1), and GATA-1. These results suggest that mouse Runx1 plays a role in the development of both primitive and definitive hematopoietic cells.
Yokomizo
Tomomasa
T
Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Japan.
Hasegawa
Kazuteru
K
Ishitobi
Hiroyuki
H
Osato
Motomi
M
Ema
Masatsugu
M
Ito
Yoshiaki
Y
Yamamoto
Masayuki
M
Takahashi
Satoru
S
D000818:Animals; D002454:Cell Differentiation; D050676:Core Binding Factor Alpha 2 Subunit / Q000172:deficiency / Q000378:metabolism*; D015536:Down-Regulation; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000378:metabolism; D004920:Erythropoiesis; D050982:GATA1 Transcription Factor / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000378:metabolism; D015017:Yolk Sac / Q000166:cytology / Q000378:metabolism
D050676:Core Binding Factor Alpha 2 Subunit; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D051741:Kruppel-Like Transcription Factors; C493726:Runx1 protein, mouse; C080774:erythroid Kruppel-like factor; D012313:RNA