title: Periodontal tissue regeneration by recombinant human transforming growth factor-beta 3 in Papio ursinus.
abstract: OBJECTIVE
  Osteogenic proteins of the transforming growth factor-beta superfamily induce periodontal tissue regeneration in animal models, including primates. To our knowledge, no studies have been performed in periodontal regeneration using the transforming growth factor-beta 3 isoform. In the present study, recombinant human transforming growth factor-beta 3 was examined for its ability to induce periodontal tissue regeneration in the nonhuman primate, Papio ursinus.
  
  
  METHODS
  Class II furcation defects were surgically created bilaterally in the maxillary and mandibular molars of four adult baboons. Heterotopic ossicles, for transplantation to selected furcation defects, were induced within the rectus abdominis muscle by recombinant human transforming growth factor-beta 3. Forty days later, the periodontal defects were implanted with recombinant human transforming growth factor-beta 3 in Matrigel as the delivery system, with recombinant human transforming growth factor-beta 3 plus minced muscle tissue in Matrigel, or with the harvested recombinant human transforming growth factor-beta 3-induced ossicles. Sixty days after periodontal implantation, the animals were killed and the specimens harvested. Histological analysis on undecalcified sections measured the area and volume of new alveolar bone and the coronal extension of newly formed alveolar bone and cementum.
  
  
  RESULTS
  Morphometric analyses showed pronounced periodontal regeneration in experimental defects compared with controls. Substantial regeneration was observed in defects implanted with fragments of heterotopically induced ossicles and with recombinant human transforming growth factor-beta 3 plus minced muscle tissue.
  
  
  CONCLUSIONS
  Recombinant human transforming growth factor-beta 3 in Matrigel significantly enhanced periodontal tissue regeneration in the nonhuman primate, P. ursinus.
authors.affiliation: Bone Research Unit, Medical Research Council/University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: J A
authors.initials: JA
authors.lastname: Teare
authors.forename: L N
authors.initials: LN
authors.lastname: Ramoshebi
authors.forename: U
authors.initials: U
authors.lastname: Ripamonti
mesh_terms: D000539:Alveolar Process / Q000033:anatomy & histology / Q000601:surgery; D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects*; D017823:Furcation Defects / Q000188:drug therapy*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D006801:Humans; D008963:Molar / Q000601:surgery*; D009999:Ossification, Heterotopic / Q000139:chemically induced; D048549:Papio ursinus; D020033:Protein Isoforms; D017568:Rectus Abdominis / Q000601:surgery; D016212:Transforming Growth Factor beta / Q000627:therapeutic use; D053782:Transforming Growth Factor beta3 / Q000627:therapeutic use*
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D020033:Protein Isoforms; D016212:Transforming Growth Factor beta; D053782:Transforming Growth Factor beta3