title: A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair.
abstract: Representing one of the most fascinating RNA polymerases, the CCA-adding enzyme (tRNA nucleotidyltransferase) is responsible for synthesis and repair of the 3'-terminal CCA sequence in tRNA transcripts. As a consequence of this important function, this enzyme is found in all organisms analyzed so far. Here, it is shown that the closely related enzymes of Homo sapiens and Escherichia coli differ substantially in their substrate preferences for the incorporation of CTP and ATP. While both enzymes require helical structures (mimicking the upper part of tRNAs) for C addition, the data indicate that the E. coli enzyme--in contrast to the human version--is quite promiscuous concerning the incorporation of ATP, where any RNA ending with two C residues is accepted. This feature is consistent with the primary function of the E. coli protein as a repair enzyme. Furthermore, even if the amino acid motif that interacts with the incoming nucleotides in the NTP binding pocket of these enzymes is destroyed and does no longer discriminate between individual bases, both nucleotidyltransferases have a back-up mechanism that ensures CCA addition with considerable accuracy and efficiency in order to guarantee functional protein synthesis and, consequently, the survival of the cell.
authors.affiliation: University of Leipzig, Institute for Biochemistry, Brüderstrasse 34, D-04103 Leipzig, Germany.
authors.forename: Esther
authors.initials: E
authors.lastname: Lizano
authors.forename: Marion
authors.initials: M
authors.lastname: Scheibe
authors.forename: Christiane
authors.initials: C
authors.lastname: Rammelt
authors.forename: Heike
authors.initials: H
authors.lastname: Betat
authors.forename: Mario
authors.initials: M
authors.lastname: Mörl
mesh_terms: D020413:3' Untranslated Regions; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D010766:Phosphorylation; D012316:RNA Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; D012360:RNA, Transfer, Phe; D012316:RNA Nucleotidyltransferases; C023198:tRNA nucleotidyltransferase