RecO is essential for DNA damage repair in Deinococcus radiodurans.
Here we present direct evidence for the vital role of RecO in Deinococcus radiodurans's radioresistance. A recO null mutant was constructed using a deletion replacement method. The mutant exhibited a growth defect and extreme sensitivity to irradiation with gamma rays and UV light. These results suggest that DNA repair in this organism occurs mainly via the RecF pathway.
Xu
Guangzhi
G
Key Laboratory of Chinese Ministry of Agriculture and Zhejiang Province for Nuclear-Agricultural Sciences, Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, China.
Wang
Liangyan
L
Chen
Huan
H
Lu
Huiming
H
Ying
Nanjiao
N
Tian
Bing
B
Hua
Yuejin
Y
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D004249:DNA Damage; D004260:DNA Repair; D034301:Deinococcus / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D005720:Gamma Rays; D009154:Mutation; D014466:Ultraviolet Rays
D001426:Bacterial Proteins