title: Functional interactions between Sae2 and the Mre11 complex.
abstract: The Mre11 complex functions in double-strand break (DSB) repair, meiotic recombination, and DNA damage checkpoint pathways. Sae2 deficiency has opposing effects on the Mre11 complex. On one hand, it appears to impair Mre11 nuclease function in DNA repair and meiotic DSB processing, and on the other, Sae2 deficiency activates Mre11-complex-dependent DNA-damage-signaling via the Tel1-Mre11 complex (TM) pathway. We demonstrate that SAE2 overexpression blocks the TM pathway, suggesting that Sae2 antagonizes Mre11-complex checkpoint functions. To understand how Sae2 regulates the Mre11 complex, we screened for sae2 alleles that behaved as the null with respect to Mre11-complex checkpoint functions, but left nuclease function intact. Phenotypic characterization of these sae2 alleles suggests that Sae2 functions as a multimer and influences the substrate specificity of the Mre11 nuclease. We show that Sae2 oligomerizes independently of DNA damage and that oligomerization is required for its regulatory influence on the Mre11 nuclease and checkpoint functions.
authors.affiliation: Laboratory of Chromosome Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
authors.forename: Hee-Sook
authors.initials: HS
authors.lastname: Kim
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Vijayakumar
authors.forename: Mike
authors.initials: M
authors.lastname: Reger
authors.forename: Jacob C
authors.initials: JC
authors.lastname: Harrison
authors.forename: James E
authors.initials: JE
authors.lastname: Haber
authors.forename: Clifford
authors.initials: C
authors.lastname: Weil
authors.forename: John H J
authors.initials: JH
authors.lastname: Petrini
mesh_terms: D004249:DNA Damage; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004720:Endonucleases; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D017353:Gene Deletion; D005800:Genes, Fungal; D008540:Meiosis / Q000235:genetics; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D004271:DNA, Fungal; C107849:SAE2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D008741:Methyl Methanesulfonate; D004706:Endodeoxyribonucleases; D004720:Endonucleases; D005090:Exodeoxyribonucleases; C094191:MRE11 protein, S cerevisiae