Delivery of nitric oxide for analysis of the function of cytochrome c'.
On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.
Cole
Lindsay J
LJ
Department of Biology, University of York, Heslington, York, United Kingdom.
Huston
Wilhelmina M
WM
Moir
James W B
JW
D045305:Cytochromes c' / Q000737:chemistry* / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D006418:Heme / Q000737:chemistry; D009345:Neisseria meningitidis / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000378:metabolism; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D045305:Cytochromes c'; D005296:Ferrous Compounds; D009569:Nitric Oxide; D006418:Heme