title: Deposits of transforming growth factor-beta-induced protein in granular corneal dystrophy type II after LASIK.
abstract: OBJECTIVE
  To analyze components of the deposits in the corneal flap interface of granular corneal dystrophy type II (GCD II) patients after laser in situ keratomileusis (LASIK).
  
  
  METHODS
  Four corneal GCD II specimens displaying disease exacerbation after LASIK were analyzed. Three of these specimens included the recipient corneal button after penetrating keratoplasty or deep lamellar keratoplasty for advanced GCD II after LASIK. The fourth specimen, a similar case of GCD II after LASIK, included the amputated corneal flap. Specimens were processed for histopathologic and immunohistochemical analyses.
  
  
  RESULTS
  Corneal stromal deposits in the LASIK flaps of all specimens were stained with 3 anti-transforming growth factor-beta-induced protein (TGFBIp) antibodies. The deposits displayed bright red color staining with Masson trichrome; however, negative staining was seen with Congo red, suggesting that hyaline is the main component localizing to the TGFBIp deposits rather than amyloid.
  
  
  CONCLUSIONS
  Amorphous granular material deposited along the interface of the LASIK flap in GCD II corneas is composed mainly of hyaline deposits.
authors.affiliation: Corneal Dystrophy Research Institute, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Tae-Im
authors.initials: TI
authors.lastname: Kim
authors.forename: Mi In
authors.initials: MI
authors.lastname: Roh
authors.forename: Hans E
authors.initials: HE
authors.lastname: Grossniklaus
authors.forename: Shin Jeong
authors.initials: SJ
authors.lastname: Kang
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hamilton
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Schorderet
authors.forename: W Barry
authors.initials: WB
authors.lastname: Lee
authors.forename: Eung Kweon
authors.initials: EK
authors.lastname: Kim
mesh_terms: D000328:Adult; D001391:Azo Compounds; D004396:Coloring Agents; D003224:Congo Red; D003317:Corneal Dystrophies, Hereditary / Q000378:metabolism* / Q000601:surgery; D003319:Corneal Stroma / Q000378:metabolism*; D004801:Eosine Yellowish-(YS); D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D020731:Keratomileusis, Laser In Situ; D015948:Keratoplasty, Penetrating; D008297:Male; D008739:Methyl Green; D008875:Middle Aged; D013194:Staining and Labeling / Q000379:methods; D013524:Surgical Flaps; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D000099222:betaIG-H3 Protein
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D003224:Congo Red; D004801:Eosine Yellowish-(YS); D016326:Extracellular Matrix Proteins; D008739:Methyl Green; D016212:Transforming Growth Factor beta; D000099222:betaIG-H3 Protein; C017768:trichrome stain