Analysis of the essential DNA region for OsEBP-89 promoter in response to methyl jasmonic acid.
In rice, the characterization of OsEBP-89 is inducible by various stress-or hormone-stimuli, including ethylene, abscisic acid (ABA), jasmonate acid (JA), drought and cold. Here, we report the investigation of essential DNA region within OsEBP-89 promoter for methyl jasmonic acid (MeJA) induction. PLACE analysis indicates that this promoter sequence contains multiple potential elements in response to various stimuli. First, we fused this promoter with GUS gene and analyzed its expression under MeJA treatment through Agrobacterium infiltration mediating transient expression in tobacco leaves. Our results revealed that this chimeric gene could be inducible by MeJA in tobacco leaves. To further determine the crucial sequences responsible for MeJA induction, we generated a series of deletion promoters which were fused with GUS reporter gene respectively. The results of transient expression of GUS gene driven by these mutant promoters show that the essential region for MeJA induction is positioned in the region between -1200 and -800 in OsEBP-89 promoter containing a G-box (-1127), which is distinct from the essential region containing ERE (-562) for ACC induction. In all, our finding is helpful in understanding the molecular mechanism of OsEBP-89 expression under different stimuli.
Li
Ang
A
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Chen
LiangLiang
L
Ren
HaiYun
H
Wang
XueChen
X
Zhang
HaiWen
H
Huang
Rong-Feng
RF
D000085:Acetates / Q000378:metabolism / Q000494:pharmacology*; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology*; D018744:DNA, Plant / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018745:Genome, Plant / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D000085:Acetates; D003517:Cyclopentanes; D018744:DNA, Plant; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate