title: [Preparation and identification of monoclonal antibodies against human DcR3].
abstract: OBJECTIVE
  To prepare monoclonal antibodies(mAbs) against human DcR3 and identify their characterization.
  
  
  METHODS
  BALB/c mice were immunized with purified His-DcR3 protein, and mAbs against DcR3 which prepared by hybridoma technique were purified and identified by their specificity, subtype, titers via ELISA and Western blot.
  
  
  RESULTS
  Five hybridoma cell lines secreting mAbs against human DcR3 were obtained, which were determined as IgG1 subtype and ascites titers of five mAbs against DcR3 reached 1x10(-5)-1x10(-7). Five mAbs were proved to recognize His-DcR3 protein specifically, one of which (1B1) could recognize SW480 cell.
  
  
  CONCLUSIONS
  mAbs against DcR3 with high titers and specificity have been prepared and purified successfully, which laid a foundation for the study of DcR3 expression, distribution in tissu and development of ELISA kit.
authors.affiliation: Department of Respiratory Diseases, Jilin University, Changchun 130041, China. feng1218@sohu.com
authors.forename: Chun-feng
authors.initials: CF
authors.lastname: Wu
authors.forename: Zhong-sen
authors.initials: ZS
authors.lastname: Ma
authors.forename: Xiu-li
authors.initials: XL
authors.lastname: Wang
authors.forename: Jun-ling
authors.initials: JL
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wu-li-ji
authors.initials: WL
authors.lastname: Tumen
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Qiao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053323:Receptors, Tumor Necrosis Factor, Member 6b / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D053323:Receptors, Tumor Necrosis Factor, Member 6b; C505911:TNFRSF6B protein, human