Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria.
We describe the development of a miniaturised microarray for the detection of antimicrobial resistance genes in Gram-negative bacteria. Included on the array are genes encoding resistance to aminoglycosides, trimethoprim, sulphonamides, tetracyclines and beta-lactams, including extended-spectrum beta-lactamases. Validation of the array with control strains demonstrated a 99% correlation between polymerase chain reaction and array results. There was also good correlation between phenotypic and genotypic results for a large panel of Escherichia coli and Salmonella isolates. Some differences were also seen in the number and type of resistance genes harboured by E. coli and Salmonella strains. The array provides an effective, fast and simple method for detection of resistance genes in clinical isolates suitable for use in diagnostic laboratories, which in future will help to understand the epidemiology of isolates and to detect gene linkage in bacterial populations.
Batchelor
Miranda
M
Department of Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey, UK.
Hopkins
Katie L
KL
Liebana
Ernesto
E
Slickers
Peter
P
Ehricht
Ralf
R
Mafura
Muriel
M
Aarestrup
Frank
F
Mevius
Dik
D
Clifton-Hadley
Felicity A
FA
Woodward
Martin J
MJ
Davies
Rob H
RH
Threlfall
E John
EJ
Anjum
Muna F
MF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D013223:Statistics as Topic
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial