Evaluation of [67Ga]-insulin for insulin receptor imaging.
BACKGROUND
Radiolabelled human recombinant insulin can be used for the imaging of insulin receptors in some tumours where FDG has natural uptake and diminishes the value of its imaging.


METHODS
Insulin was successively labelled with [(67)Ga]-gallium chloride after conjugation with freshly prepared cyclic DTPA-dianhydride (HPLC radiochemical purity assay > 96%) followed by biodistribution studies in normal rats, white blood cell labelling and preliminary SPECT studies.


RESULTS
In vitro studies demonstrated the retention of radiolabelled insulin receptor affinity using freshly prepared human white blood cells at different blood sugar conditions. Preliminary in vivo studies in a normal rat model was performed to determine the biodistribution of the radioimmunoconjugate at up to 44 h. SPECT images revealed high uptake of the liver.


CONCLUSIONS
Radiolabelled insulin is stable enough to be used in biological studies in order to image insulin receptors in diabetic conditions as well as possible tumour imaging applications. The data was consistent with other radiolabelled insulin studies.
Jalilian
Amir Reza
AR
Nuclear Medicine Group, Agriculture, Medicine and Industrial Research School, Karaj, Iran. ajalilian@nrcam.org
Garosi
Javad
J
Gholami
Ebrahim
E
Akhlaghi
Mehdi
M
Saddadi
Fariba
F
Bolourinovin
Fatemeh
F
Karimian
Alireza
A
D000818:Animals; D002478:Cells, Cultured; D005710:Gallium Radioisotopes / Q000737:chemistry / Q000493:pharmacokinetics; D007328:Insulin / Q000493:pharmacokinetics*; D007962:Leukocytes / Q000000981:diagnostic imaging* / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism*; D014018:Tissue Distribution
D005710:Gallium Radioisotopes; D007328:Insulin; D019275:Radiopharmaceuticals; D011972:Receptor, Insulin