[Effects of amitrole on thyroid hormone-associated gene transcription in FRTL-5 cells].
OBJECTIVE
To observe the effects of amitrole on the transcription of thyroglobulin (tg), thyroid peroxidase (tpo), Na(+)/I- symporter (nis), Na(+)/I- symporter (nis), thyroid-stimulating hormone receptor (tshr), thyroid transcription factor 1 (ttf-1) and paired-domain protein-8 (pax-8) genes in FRTL-5 cells and investigate the mechanism of amitrole for intervening in thyroid hormone activity.


METHODS
FRTL-5 cells were treated with amitrole at 0.001, 0.01 and 0.1 mg/ml for 24 h, respectively, after which the cells were collected for extraction of the total RNA. RT-PCR was used to examine the effects of amitrole on the transcription of tg, tpo, nis, tshr, pax-8 and ttf-1 genes in FRTL-5 cells.


RESULTS
Amitrole significantly induced tg gene transcription at all the doses, but produced no obvious effects on tpo and nis gene transcription. At the concentration of 0.1 mg/ml, amitrole significantly reduced pax-8 and tshr gene transcription but increased ttf-1 gene transcription.


CONCLUSIONS
The effects of amitrole on thyroid hormone activity may be related with its actions on tg, ttf-1, tshr and pax-8 gene transcription.
Pan
Hong-Mei
HM
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China. happyphm@163.com
Zhang
Li-Shi
LS
Wu
De-Sheng
DS
D000640:Amitrole / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000633:toxicity; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics*; D051381:Rats; D011916:Rats, Inbred F344; D011989:Receptors, Thyrotropin / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013954:Thyroglobulin / Q000235:genetics*; D013961:Thyroid Gland / Q000166:cytology; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D004791:Enzyme Inhibitors; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; D011989:Receptors, Thyrotropin; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D013954:Thyroglobulin; D000640:Amitrole