title: A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae.
abstract: Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.
authors.affiliation: Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
authors.forename: Grace Marie
authors.initials: GM
authors.lastname: Jones
authors.forename: Jim
authors.initials: J
authors.lastname: Stalker
authors.forename: Sean
authors.initials: S
authors.lastname: Humphray
authors.forename: Anthony
authors.initials: A
authors.lastname: West
authors.forename: Tony
authors.initials: T
authors.lastname: Cox
authors.forename: Jane
authors.initials: J
authors.lastname: Rogers
authors.forename: Ian
authors.initials: I
authors.lastname: Dunham
authors.forename: Gregory
authors.initials: G
authors.lastname: Prelich
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*