title: Delivery of nitric oxide for analysis of the function of cytochrome c'.
abstract: On delivery of nitric oxide (NO) to protein samples (e.g., cytochrome c'), for spectroscopic experiments it is important to avoid exposure to oxygen and to remove contaminants from the NO gas. We describe a number of techniques for steady-state UV/Vis spectrophotometry and pre-steady-state stopped-flow spectrophotometry analysis of cytochrome c'.
authors.affiliation: Department of Biology, University of York, Heslington, York, United Kingdom.
authors.forename: Lindsay J
authors.initials: LJ
authors.lastname: Cole
authors.forename: Wilhelmina M
authors.initials: WM
authors.lastname: Huston
authors.forename: James W B
authors.initials: JW
authors.lastname: Moir
mesh_terms: D045305:Cytochromes c' / Q000737:chemistry* / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism; D006418:Heme / Q000737:chemistry; D009345:Neisseria meningitidis / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000378:metabolism; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D045305:Cytochromes c'; D005296:Ferrous Compounds; D009569:Nitric Oxide; D006418:Heme