Preparation of uniform rich cholesterol unilamellar nanovesicles using CO2-expanded solvents.
Nanoscopic and uniform unilamellar vesicles, rich in cholesterol, have been prepared by a new procedure, named "DELOS-SUSP", which is based on the depressurization of a cholesterol solution in CO2-expanded acetone into an aqueous solution containing a surfactant. The CO2 is used here as a cosolvent medium, allowing the straightforward preparation of vesicular systems with controlled size distribution, uniform shape, and stability unachievable by conventional mixing technologies. The resulting nanoscopic vesicular systems dispersed in water were characterized using dynamic light scattering, zeta potential, turbidity, and cryogenic transmission electron microscopy. The influence of operational parameters of this new methodology on the physicochemical characteristics of the vesicular systems is also reported.
Cano-Sarabia
Mary
M
Institut de Ciència de Materials de Barcelona (CSIC)-CIBER-BBN, Campus Universitario de Bellaterra, 08193 Cerdanyola del Vallés, Spain.
Ventosa
Nora
N
Sala
Santiago
S
Patiño
Cristina
C
Arranz
Rocío
R
Veciana
Jaume
J
D000096:Acetone / Q000737:chemistry; D002245:Carbon Dioxide / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D012996:Solutions / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D014867:Water / Q000737:chemistry
D012996:Solutions; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D000096:Acetone; D002245:Carbon Dioxide; D002784:Cholesterol