Structural analysis of the O-polysaccharide from the lipopolysaccharide of Azospirillum brasilense S17.
A mixture of two structurally distinct neutral O-polysaccharides was obtained by mild acid degradation of the lipopolysaccharide isolated by the phenol/water extraction from the asymbiotic diazotrophic rhizobacterium Azospirillum brasilense S17. The following structures of the O-polysaccharides were established by composition and methylation analyses, Smith degradation, and 1H and 13C NMR spectroscopy, including a 2D NOESY experiment: [Formula: see text] where L-Rha2Me stands for 2-O-methyl-L-rhamnose and SHb for the (S)-3-hydroxybutanoyl group. The occurrence of two distinct polysaccharides is reported for the first time in Azospirillum spp.
Fedonenko
Yuliya P
YP
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.
Konnova
Olga N
ON
Zdorovenko
Evelina L
EL
Konnova
Svetlana A
SA
Zatonsky
George V
GV
Shashkov
Alexander S
AS
Ignatov
Vladimir V
VV
Knirel
Yuriy A
YA
D015806:Azospirillum brasilense / Q000737:chemistry*; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data
D008070:Lipopolysaccharides