Transfection of cultured eukaryotic cells using cationic lipid reagents.
The development of high-efficiency methods for the introduction of functional genetic material into eukaryotic cells using cationic lipids has accelerated biological research in the studies of gene expression, control of cell growth, and cell lineage. Transfection mediated by cationic lipids is commonly used in industrial protein production as well as in some clinical gene therapy protocols. Replacing our previous unit on this topic, this new version describes how to perform transfection of adherent and suspension cells, insect cells, and RNA transfection using the cationic lipid system.
Hawley-Nelson
Pamela
P
Life Technologies, Inc., Rockville, Maryland, USA.
Ciccarone
Valentina
V
D000818:Animals; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D015203:Reproducibility of Results; D014162:Transfection / Q000379:methods*
D002412:Cations; D008055:Lipids; D004247:DNA