Cascade of extracytoplasmic function sigma factors in Mycobacterium tuberculosis: identification of a sigmaJ-dependent promoter upstream of sigI.
A previously optimized Escherichia coli two-plasmid system was used to identify promoters recognized by RNA polymerase containing the Mycobacterium tuberculosis extracytoplasmic function (ECF) sigma factor sigma(J). The only positive clone revealed a promoter directing expression of the sigI gene encoding ECF sigma factor sigma(I). The transcriptional start point of the promoter was determined and the promoter was confirmed by an in vitro transcription assay with either E. coli or Mycobacterium smegmatis core RNA polymerase and isolated sigma(J).
Homerova
Dagmar
D
Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Halgasova
Lenka
L
Kormanec
Jan
J
D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases