In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging. Laboratory investigation.
OBJECTIVE
Mesenchymal stem cells (MSCs) have been shown to migrate toward tumors, but their distribution pattern in gliomas has not been completely portrayed. The primary purpose of the study was to assay the tropism capacity of MSCs to gliomas, to delineate the pattern of MSC distribution in gliomas after systemic injection, and to track the migration and incorporation of magnetically labeled MSCs using 1.5-T magnetic resonance (MR) imaging.


METHODS
The MSCs from Fischer 344 rats were colabeled with superparamagnetic iron oxide nanoparticles (SPIO) and enhanced green fluorescent protein (EGFP). The tropism capacity of MSCs was quantitatively assayed in vitro using the Transwell system. To track the migration of MSCs in vivo, MR imaging was performed both 7 and 14 days after systemic administration of labeled MSCs. After MR imaging, the distribution patterns of MSCs in rats with gliomas were examined using Prussian blue and fluorescence staining.


RESULTS
The in vitro study showed that MSCs possessed significantly greater migratory capacity than fibroblast cells (p<0.001) and that lysis of F98 glioma cells and cultured F98 cells showed a greater capacity to induce migration of cells than other stimuli (p<0.05). Seven days after MSC transplantation, the SPIO-EGFP colabeled cells were distributed throughout the tumor, where a well-defined dark hypointense region was represented on gradient echo sequences. After 14 days, most of the colabeled MSCs were found at the border between the tumor and normal parenchyma, which was represented on gradient echo sequences as diluted amorphous dark areas at the edge of the tumors.


CONCLUSIONS
This study demonstrated that systemically transplanted MSCs migrate toward gliomas with high specificity in a temporal-spatial pattern, which can be tracked using MR imaging.
Wu
Xing
X
Department of Neurosurgery, Huashan Hospital, Fudan University, China. bttnirvina@gmail.com
Hu
Jin
J
Zhou
Liangfu
L
Mao
Ying
Y
Yang
Bojie
B
Gao
Liang
L
Xie
Rong
R
Xu
Feng
F
Zhang
Dong
D
Liu
Jun
J
Zhu
Jianhong
J
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D004396:Coloring Agents; D003287:Contrast Media; D003911:Dextrans; D005295:Ferrocyanides; D052203:Ferrosoferric Oxide; D005347:Fibroblasts / Q000473:pathology; D005456:Fluorescent Dyes; D005910:Glioma / Q000473:pathology*; D049452:Green Fluorescent Proteins; D007501:Iron; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000473:pathology*; D053758:Nanoparticles; D009368:Neoplasm Transplantation; D010087:Oxides; D051381:Rats; D011916:Rats, Inbred F344; D018523:Tropism / Q000502:physiology
D004396:Coloring Agents; D003287:Contrast Media; D003911:Dextrans; D005295:Ferrocyanides; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D010087:Oxides; C485184:enhanced green fluorescent protein; C097921:ferumoxtran-10; D049452:Green Fluorescent Proteins; D007501:Iron; C000170:ferric ferrocyanide; D052203:Ferrosoferric Oxide