title: In vitro transcription.
abstract: In vitro analysis of transcription and the factors that play a role in transcription require preparation of an extract that faithfully reproduces in vivo transcription. This unit describes protocols for generating extracts of mammalian cells and Drosophila embryos that are competent for in vitro transcription reactions using naked DNA or chromatin templates and for primer extension analysis of transcripts.
authors.affiliation: NICHD, Bethesda, Maryland, USA.
authors.forename: R T
authors.initials: RT
authors.lastname: Kamakaka
authors.forename: W L
authors.initials: WL
authors.lastname: Kraus
mesh_terms: D000818:Animals; D002457:Cell Extracts; D004330:Drosophila; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008967:Molecular Biology / Q000379:methods*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D002457:Cell Extracts