Surface-induced dissociation of peptides and protein complexes in a quadrupole/time-of-flight mass spectrometer.
A novel in-line surface-induced dissociation (SID) device was designed and implemented in a commercial QTOF instrument (Waters/Micromass QTOF II). This new setup allows efficient SID for a broad range of molecules. It also allows direct comparison with conventional collision-induced dissociation (CID) on the same instrument, taking advantage of the characteristics of QTOF instrumentation, including extended mass range, improved sensitivity, and better resolution compared with quadrupole analyzers and ion traps. Various peptides and a noncovalent protein complex have been electrosprayed and analyzed with the new SID setup. Here we present SID of leucine enkephalin, fibrinopeptide A, melittin, insulin chain-B, and a noncovalent protein complex from wheat, heat shock protein 16.9. The SID spectra were also compared to CID spectra. With the SID setup installed, ion transmission proved to be efficient. SID fragmentation patterns of peptides are, in general, similar to CID, with differences in the relative intensities of some peaks such as immonium ions, backbone cleavage b- versus y-type ions, and y- versus y-NH3 ions, suggesting enhanced accessibility to high-energy/secondary fragmentation channels with SID. Furthermore, these results demonstrate that the in-line SID setup is a valid substitute for CID, with potential advantages for activation of singly/multiply charged peptides and larger species such as noncovalent protein complexes.
Galhena
Asiri S
AS
Department of Chemistry, University of Arizona, 1306E University Boulevard, Tucson, Arizona 85721, USA.
Dagan
Shai
S
Jones
Christopher M
CM
Beardsley
Richard L
RL
Wysocki
Vicki H
VH
D000818:Animals; D002417:Cattle; D004743:Enkephalin, Leucine / Q000737:chemistry; D005344:Fibrinopeptide A / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation*; D008555:Melitten / Q000737:chemistry; D046912:Multiprotein Complexes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013499:Surface Properties
D007328:Insulin; D046912:Multiprotein Complexes; D010455:Peptides; D008555:Melitten; D005344:Fibrinopeptide A; D004743:Enkephalin, Leucine