Analysis of antibody response by temperature-sensitive measles vaccine strain in the cotton rat model.
Measles virus (MeV) vaccine strain, AIK-C, is temperature sensitive (ts), which is thought to be associated with attenuation of virus pathogenicity. In this study, replication and antibody response were examined in cotton rats using viruses carrying different forms of the P gene, which is responsible for the ts phenotype of strain AIK-C and its parental Edmonston strain. When cotton rats were inoculated intranasally, ts viruses neither replicated in lungs, nor reproducibly generated an antibody response. When inoculated intramusculary (i.m.), however, ts strains raised an antibody titer in all animals. This response was not observed when ultraviolet-inactivated virus was used. ts virus, inoculated i.m., was recovered from cotton rat drainage lymph nodes. These results suggest that ts virus, inoculated i.m., could replicate in the cotton rat, presumably at the superficial lymph node, and induce an antibody response. Therefore, cotton rats can serve as a small-animal model for investigating immune responses to safer ts vaccine, as well as recombinant vaccine using AIK-C as a vector for protection against other infectious agents.
Haga
Takeshi
T
Department of Veterinary Microbiology, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. a0d518u@cc.miyazaki-u.ac.jp
Murayama
Niho
N
Shimizu
Yuya
Y
Saito
Akatsuki
A
Sakamoto
Takumi
T
Morita
Tetsuo
T
Komase
Katsuhiro
K
Nakayama
Tetsuo
T
Uchida
Kazuyuki
K
Katayama
Tetsuro
T
Shinohara
Akio
A
Koshimoto
Chihiro
C
Sato
Hiroshi
H
Miyata
Hironori
H
Katahira
Kiyoaki
K
Goto
Yoshitaka
Y
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005260:Female; D008168:Lung / Q000276:immunology / Q000821:virology; D008198:Lymph Nodes / Q000276:immunology* / Q000821:virology; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D016205:Sigmodontinae / Q000276:immunology* / Q000821:virology; D013696:Temperature; D014779:Virus Replication / Q000276:immunology
D008458:Measles Vaccine