Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli.
Allophycocyanin (APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit (holo-ApcA) was constructed, resulting in over-expression of a fluorescent holo-ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 g l(-1), and the expression of holo-HT-ApcA was up to 1 g l(-1) broth. The recombinant protein showed similar spectral features to native APC.
Yang
Yu
Y
Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, Shandong, P.R. China.
Ge
Baosheng
B
Guan
Xiangyu
X
Zhang
Weijie
W
Qin
Song
S
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D004926:Escherichia coli / Q000378:metabolism; D005453:Fluorescence; D005822:Genetic Vectors; D010798:Phycocyanin / Q000096:biosynthesis* / Q000302:isolation & purification; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D053545:Spirulina / Q000378:metabolism; D046939:Synechocystis / Q000378:metabolism
D001426:Bacterial Proteins; D011994:Recombinant Proteins; C002497:allophycocyanin; D010798:Phycocyanin