Isolation of ckit-positive cardiosphere-forming cells from human atrial biopsy.
There is increasing interest in developing cell-based therapies to regenerate functional muscle and blood vessels in infarcted dysfunctional myocardium, using stem cells resident in the adult heart. The objective of our study was to identify an easy and cost-effective method for the isolation and expansion of human adult cardiac-resident stem cells. The cells were isolated from right atrial biopsy samples obtained from patients with ischemic heart disease, who were undergoing coronary artery bypass grafting. Two different isolation methods, enzymatic and nonenzymatic, were employed. The cell yield and cluster formation were not significantly different with either of the techniques used for cell isolation. The nonenzymatic method is recommended because of its simplicity and lower cost compared to the enzymatic method.
Aghila Rani
Koippallil G
KG
Division of Cellular & Molecular Cardiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, India.
Jayakumar
Karunakaran
K
Srinivas
Gopal
G
Nair
Renuka R
RR
Kartha
Chandrasekharan C
CC
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000328:Adult; D053687:Adult Stem Cells / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D001706:Biopsy; D002448:Cell Adhesion; D002449:Cell Aggregation; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D006325:Heart Atria / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism / Q000473:pathology*; D018995:Myosin Heavy Chains / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D018874:Spheroids, Cellular; D013997:Time Factors; D019210:Troponin I / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018952:Antigens, CD34; D019210:Troponin I; D014841:von Willebrand Factor; D019009:Proto-Oncogene Proteins c-kit; D018995:Myosin Heavy Chains