title: [Protective effect of NEP on Abeta-induced apoptosis in PC12 cells].
abstract: OBJECTIVE
  To identify the effect of NEP on Abeta -induced apoptosis in PC12 cells.
  
  
  METHODS
  PC12 cells that stably express NEP is generated and the effect of NEP on the process of apoptosis induced by Abeta is analyzed, including the viability of the cells, the production of LDH, ROS and ATP, the activity of Caspase-3.
  
  
  RESULTS
  NEP could improve the viability of cells and the production of ATP, inhibit the release of LDH and ROS. In the same time, the activity of caspase-3 descended (P < 0.05). But iNEP had not significant effect on cells apoptosis (P > 0.05).
  
  
  CONCLUSIONS
  NEP has the protective effect on Abeta-induced apoptosis in PC12 cells.
authors.affiliation: Biochemistry and Molecular Biology Department, School of Medicine, Shandong University, Jinan, 250012, China.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Zeng
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yang
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Kong
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Hu
authors.forename: Xing
authors.initials: X
authors.lastname: Cui
mesh_terms: D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D014162:Transfection
chemical_list: D016229:Amyloid beta-Peptides; D010450:Endopeptidases; C505410:Casp3 protein, rat; D053148:Caspase 3