Diagnostic potential of oxidative stress markers in children and adolescents with type 1 diabetes.
OBJECTIVE
To validate the diagnostic utility of oxidative stress markers in the evaluation of young type 1 diabetics, as suggested elsewhere.


METHODS
Advanced oxidation protein products (AOPP), thiobarbituric acid-reactive substances (TBARS) and total antioxidant status (TAS) were measured in sera from diabetics, their siblings and controls, with diagnostic potential evaluated by ROC analysis, and related to diabetes clinical parameters.


RESULTS
In diabetics AOPP and TBARS were elevated, TAS decreased. Similar alterations were observed for AOPP and TAS in their siblings. AOPP and TAS were good indicators of diabetes. AOPP and TBARS correlated with HbA1C (independent predictor), but were poor markers of non-adequate glycemic control. The cardiovascular disease risk factors were independent predictors of TBARS concentrations.


CONCLUSIONS
AOPP accumulation and TAS reduction seem to precede diabetes and might be considered as susceptibility indicators in relatives, but not as diabetes markers in general population (no diabetes specificity has been shown). Application in monitoring of metabolic control is not validated.
Krzystek-Korpacka
Malgorzata
M
Department of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland. krzystek@bioch.am.wroc.pl
Salmonowicz
Barbara
B
Boehm
Dorota
D
Berdowska
Izabela
I
Zielinski
Bogdan
B
Patryn
Eliza
E
Noczynska
Anna
A
Gamian
Andrzej
A
D000293:Adolescent; D015415:Biomarkers / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000209:etiology; D016022:Case-Control Studies; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000378:metabolism*; D003925:Diabetic Angiopathies / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000097:blood / Q000378:metabolism; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011236:Prediabetic State / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D012307:Risk Factors; D012680:Sensitivity and Specificity; D035781:Siblings; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D017392:Thiobarbituric Acid Reactive Substances; C517652:hemoglobin A1c protein, human