Induction of oxidative DNA damage by Helicobacter pylori in HT29 cells.
Infection with Helicobacter pylori has been associated with the development of gastric adenocarcinoma in humans. Several routes have been implicated, the main one being oxidative DNA damage resulting from chronic inflammation, which accompanies infection. However, DNA has been demonstrated in human cells after in vitro incubation with H. pylori sonicates. Using the fragment length analysis using restriction enzymes (FLARE) assay, this study investigates the DNA damaging potential of three clinical isolates of H. pylori on cultured HT29 cells. Significant amounts of oxidative DNA damage were detected in HT29 cells following a 72-hour incubation with each H. pylori isolate. As tumour induction is a known consequence of oxidative DNA damage, chronic infection with the organism may lead to the development of adenocarcinoma of the stomach.
Coffils
M
M
School of Life Science, Kingston University, Penrhyn Road, Kingston upon Thames, UK.
Jewell
A P
AP
Jones
L A
LA
Fielder
M D
MD
D000230:Adenocarcinoma / Q000150:complications / Q000235:genetics / Q000382:microbiology*; D004249:DNA Damage / Q000235:genetics / Q000502:physiology*; D053938:DNA Fragmentation; D019073:HT29 Cells; D016481:Helicobacter Infections / Q000150:complications* / Q000235:genetics / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D013274:Stomach Neoplasms / Q000150:complications / Q000235:genetics / Q000382:microbiology*