[Extract process of cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba].
OBJECTIVE
To study the extract process of Cyclic adenosinemonophosphate (cAMP) in Ziziphus jujuba.


METHODS
CAMP was extracted with water, separated and purificated by ion exchange and silica gel G column chromatography.


RESULTS
Thin layer chromatography (TLC) of the extracts and control cAMP showed the same Rf 0.75; The UV-absorption of the extracts and control cAMP had maximal absorb peak at 260 nm and the least absorb value at 230 nm; Infra-red spectrum of the extracts was indistinguishable from that of control cAMP; Retention time of the extracts by high-performance liquid chromatography (HPLC) determinate was 5.237 minute,unsettled impurity peak,content was 97%. Control cAMP was 5.350 minute,their retention time were of little difference.


CONCLUSIONS
Chemistry structure of this extract with that of control cAMP is the same.
Li
Ming
M
Tianjin Institute of Medical and Pharmaceutical Sciences, Tianjin 300020, China.
Yang
Guo-lin
GL
Mi
Sha
S
Gao
Xiang-yun
XY
Wang
Ying
Y
Li
Ming-run
MR
D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000737:chemistry / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*; D005782:Gels; D007475:Ion Exchange Resins; D015394:Molecular Structure; D010945:Plants, Edible / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D031957:Ziziphus / Q000737:chemistry*
D005782:Gels; D007475:Ion Exchange Resins; D000242:Cyclic AMP