title: Germline expression of mammalian CTF18, an evolutionarily conserved protein required for germ cell proliferation in the fly and sister chromatid cohesion in yeast.
abstract: Cutlet/CTF18 encodes an evolutionarily conserved protein that is crucial for germline development in Drosophila melanogaster. Loss of function of cutlet in the fly results in a sterile phenotype due to the failure of germline stem cells to proliferate. CTF18 was first identified in Saccharomyces cerevisiae as a sister chromatid cohesion factor that is essential for the faithful transmission of chromosomes during DNA replication. We have cloned and characterized the human and mouse CTF18 orthologs of the D. melanogaster gene, cutlet. We have demonstrated that CTF18 mRNA is expressed in human and mouse testis and ovary, and that CTF18 protein is expressed throughout the male and female germline of the mouse. We suggest a unique biological role for CTF18 in mammalian germ cell development based on its mammalian germline expression, high degree of evolutionary conservation, and role in DNA replication and chromosomal stability in yeast.
authors.affiliation: Center for Research on Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6160, USA. kberkowitz@obgyn.upenn.edu
authors.forename: Karen M
authors.initials: KM
authors.lastname: Berkowitz
authors.forename: Klaus H
authors.initials: KH
authors.lastname: Kaestner
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Jongens
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D002842:Chromatids / Q000235:genetics; D004330:Drosophila; D019143:Evolution, Molecular; D005260:Female; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid; D012854:Sister Chromatid Exchange; D013737:Testis / Q000378:metabolism
chemical_list: C477290:CHTF18 protein, human; D002352:Carrier Proteins; D009687:Nuclear Proteins; D000074183:ATPases Associated with Diverse Cellular Activities