Synthesis of fluorous tags for incorporation of reducing sugars into a quantitative microarray platform.
Carbohydrate microarrays can map out key interactions of carbohydrates and proteins in a high-throughput manner, but require the inclusion of a range of sugars for their optimal use. Here we present the synthesis and use of a new hydroxylamine-modified fluorous tag that allows the facile incorporation of reducing sugars into a noncovalent fluorous-based microarray after simple purification by fluorous solid-phase extraction (FSPE). The microarray supports quantitative screening against carbohydrate-binding proteins.
Chen
Guo-Song
GS
Department of Chemistry and the Plant Sciences Institute, Gilman Hall, Iowa State University, Ames, Iowa 50011-3111, USA.
Pohl
Nicola L
NL
D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry*; D006031:Glycosylation; D006845:Hydrocarbons, Fluorinated / Q000138:chemical synthesis* / Q000737:chemistry; D019811:Hydroxylamine / Q000737:chemistry; D046228:Microarray Analysis / Q000379:methods*; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D013237:Stereoisomerism; D013499:Surface Properties
D002241:Carbohydrates; D006845:Hydrocarbons, Fluorinated; D011506:Proteins; D019811:Hydroxylamine