Rhizoremediation of cadmium soil using a cadmium-resistant plant growth-promoting rhizopseudomonad.
Three pseudomonad strains (MKRh1, MKRh3, and MKRh4) isolated from rhizospheres showed a high growth potential in the presence of cadmium, with a minimal inhibitory concentration of 7 mM for cadmium chloride (CdCl(2)). Among them, isolate MKRh3 was specifically chosen as the most favorable cadmium-resistant plant growth-promoting rhizobacterium based on its higher 1-aminocyclopropane carboxylic acid deaminase activity, siderophore production, phosphate solubilization, and auxin synthesis and the in vivo growth increment of black gram plants. 16S ribosomal RNA gene sequencing identified MKRh3 as Pseudomonas aeruginosa. The effect of cadmium on black gram plants was studied in soil amended with a gradient of CdCl(2) concentration and the toxicity was evident from stunted growth, poor rooting, and cadmium accumulation. Application of isolate MKRh3 by seed coating overcomes the cadmium toxicity; plants showed lessened cadmium accumulation, extensive rooting, and enhanced plant growth. Further research and development of this promising innate strategy for scale-up to higher-efficiency and large-scale application will be a potent tool to prevent accumulation of cadmium in plants, thereby ensuring food security for humans.
Ganesan
Velmurugan
V
Department of Biochemistry, Madurai Kamaraj University, Madurai, 625 021, India. oomvel@gmail.com
D000598:Amino Acids, Cyclic / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D024881:Drug Resistance, Bacterial; D052918:Environmental Restoration and Remediation / Q000379:methods*; D007887:Fabaceae / Q000187:drug effects / Q000254:growth & development* / Q000382:microbiology; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000096:biosynthesis / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D017262:Siderophores / Q000096:biosynthesis; D012988:Soil Microbiology
D000598:Amino Acids, Cyclic; D007210:Indoleacetic Acids; D010710:Phosphates; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; D002104:Cadmium; C023863:1-aminocyclopropane-1-carboxylic acid