title: Secretion of inhibin in male Japanese quail (Coturnix japonica) from one week of age to sexual maturity.
abstract: The objective of this study was to investigate the changes in secretion of inhibin and cellular localization of the inhibin alpha and inhibin/activin (beta(A) and beta(B)) subunits in male Japanese quail from 1 to 7 weeks after hatching. The post-hatch profile of plasma luteinizing hormone (LH), immunoreactive (ir) inhibin and testosterone were measured by radioimmunoassay. Testes were immunostained by the avidin-biotin-peroxidase complex method (ABC) using polyclonal antisera raised against inhibin alpha, inhibin/activin beta(A) and inhibin/activin beta(B) from one week of age to sexual maturity. Testicular weight increased gradually until 4 weeks and abruptly increased from 5 weeks of age onwards. The plasma concentrations of LH and ir-inhibin increased significantly at 5 weeks of age, and the plasma concentration of testosterone increased significantly at 6 weeks of age. Pituitary contents of LH showed a steady increase until 6 weeks of age and then abruptly increased at 7 weeks of age. Coincident to the increase in plasma testosterone, the testicular contents of testosterone significantly increased from 5 weeks through sexual maturity. Immunohistochemically, localization of the inhibin/activin alpha, beta(A) and beta(B) subunits was found in the Sertoli and Leydig cells at all ages of development from one week of age to sexual maturity. These results suggest that Sertoli and Leydig cells are the major source of inhibin secretion during development in male Japanese quail.
authors.affiliation: Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
authors.forename: Manila
authors.initials: M
authors.lastname: Sedqyar
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Mohamed M M
authors.initials: MM
authors.lastname: Kandiel
authors.forename: Sinji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Akira K
authors.initials: AK
authors.lastname: Suzuki
authors.forename: Shinji
authors.initials: S
authors.lastname: Taneda
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D000818:Animals; D001360:Avidin / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D001835:Body Weight; D002417:Cattle; D003370:Coturnix; D028322:Inhibin-beta Subunits / Q000097:blood* / Q000378:metabolism; D007265:Inhibins / Q000097:blood* / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D008954:Models, Biological; D009929:Organ Size; D010544:Peroxidases / Q000378:metabolism; D013045:Species Specificity; D013737:Testis / Q000473:pathology
chemical_list: C103008:inhibin beta A subunit; C094377:inhibin-alpha subunit; D001360:Avidin; D007265:Inhibins; D001710:Biotin; D007986:Luteinizing Hormone; D028322:Inhibin-beta Subunits; D010544:Peroxidases