title: Immunohistochemistry.
abstract: Immunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright-field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided.
authors.affiliation: Bio-Reference Laboratories, Inc., Elmwood Park, New Jersey, USA.
authors.forename: Marsha
authors.initials: M
authors.lastname: Goldstein
authors.forename: Simon
authors.initials: S
authors.lastname: Watkins
mesh_terms: D000096:Acetone; D000818:Animals; D001710:Biotin / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D016612:Paraffin Embedding; D009195:Peroxidase / Q000378:metabolism; D013194:Staining and Labeling; D019809:Streptavidin / Q000378:metabolism
chemical_list: D000096:Acetone; D001710:Biotin; D019809:Streptavidin; D009195:Peroxidase