title: Associations of interleukin-6 with interleukin-1beta, interleukin-8 and macrophage inflammatory protein-1beta in midlife women.
abstract: OBJECTIVE
  The aim of the present study was to determine the associations of interleukin (IL)-6 with other cytokines and chemokines and to compare these associations in peri- and postmenopausal women.
  
  
  METHODS
  Ninety-nine perimenopausal and 92 postmenopausal women were enrolled in this study. Serum concentrations of IL-6, IL-1beta, IL-2, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, tumor necrosis factor (TNF)-alpha, interferon gamma, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage (GM)-CSF, macrophage inflammatory protein (MIP)-1beta and monocyte chemotactic protein (MCP)-1 were measured simultaneously using a multiplexed cytokine assay.
  
  
  RESULTS
  Among the 17 cytokines, IL-6, IL-1beta, IL-5, IL-7, IL-8, IL-10, MCP-1 and MIP-1beta were detected in serum in more than 50% of the women. Serum levels of IL-4 and MCP-1 in postmenopausal women were significantly higher than those in perimenopausal women. Serum IL-6 concentrations showed significant and positive correlations with serum concentrations of IL-1beta, IL-8, MIP-1beta, IL-7 and MCP-1 in women regardless of menopausal status, and these correlations were still significant after adjustment for age and body mass index.
  
  
  CONCLUSIONS
  Serum IL-6 concentration was found to be closely associated with serum concentrations of IL-1beta, IL-8, MIP-1beta, IL-7 and MCP-1 in women regardless of menopausal status, suggesting that these cytokines act in concert with the progression of several symptoms and various diseases.
authors.affiliation: Department of Obstetrics and Gynecology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan. yasui@clin.med.tokushima-u.ac.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yasui
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Uemura
authors.forename: Masayo
authors.initials: M
authors.lastname: Yamada
authors.forename: Toshiya
authors.initials: T
authors.lastname: Matsuzaki
authors.forename: Naoko
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Masamichi
authors.initials: M
authors.lastname: Noguchi
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Yuzurihara
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kase
authors.forename: Minoru
authors.initials: M
authors.lastname: Irahara
mesh_terms: D054407:Chemokine CCL4 / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood; D008875:Middle Aged; D047648:Perimenopause / Q000097:blood / Q000276:immunology*; D017698:Postmenopause / Q000097:blood / Q000276:immunology*
chemical_list: D054407:Chemokine CCL4; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8