title: Effect of chengzai pill on the L-type Ca2+ channel currents of osteoblasts pretreated with methylprednisolone.
abstract: OBJECTIVE
  To observe the effect of the serum containing Chengzai Pill on the L-type voltage-sensitive calcium channels current (L-VSCCsC) of osteoblastic MC3T3-E1 cells pretreated with methylprednisolone (mPSL).
  
  
  METHODS
  A control group, a model group, a low dose group and a high dose group were set up. The whole cell patch clamp technique was used to record L-VSCCsC of 10 osteoblastic MC3T3-E1 cells in each group and their peak currents were determined.
  
  
  RESULTS
  The peak current of the control group was 0.2284 +/- 0.0209 nA; the peak current of the model group was 0.1839 +/- 0.0179 nA; decreased by 19.5% as compared with the control group (P<0.01); the peak current of the low and high dose groups was 0.2526 +/- 0.0093 nA and 0.2671 +/- 0.0120 nA respectively, increased by 37.4% and 45.2% as compared with the model group (P<0.01); the difference between the low and high dose groups was P<0.05.
  
  
  CONCLUSIONS
  1. mPSL inhibits L-VSCCsC of osteoblasts; and 2. The serum containing Chengzai Pill increases L-VSCCsC of osteoblasts pretreated with mPSL.
authors.affiliation: Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chen
authors.forename: Bingshen
authors.initials: B
authors.lastname: Jia
authors.forename: Keqin
authors.initials: K
authors.lastname: Huang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.forename: Chongguang
authors.initials: C
authors.lastname: Zhou
authors.forename: Wanqiang
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000502:physiology*; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004594:Electrophysiology; D008775:Methylprednisolone / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology*
chemical_list: D020746:Calcium Channels, L-Type; D004365:Drugs, Chinese Herbal; D002118:Calcium; D008775:Methylprednisolone