title: Human podocytes adhere to the KRGDS motif of the alpha3alpha4alpha5 collagen IV network.
abstract: Podocyte adhesion to the glomerular basement membrane is required for proper function of the glomerular filtration barrier. However, the mechanism whereby podocytes adhere to collagen IV networks, a major component of the glomerular basement membrane, is poorly understood. The predominant collagen IV network is composed of triple helical protomers containing the alpha3alpha4alpha5 chains. The protomers connect via the trimeric noncollagenous (NC1) domains to form hexamers at the interface. Because the NC1 domains of this network can potentially support integrin-dependent cell adhesion, it was determined whether individual NC1 monomers or alpha3alpha4alpha5 hexamers support podocyte adhesion. It was found that, although human podocytes did not adhere to NC1 domains proper, they did adhere via integrin alphavbeta3 to a KRGDS motif located adjacent to alpha3NC1 domains. Because the KRGDS motif is a site of phosphorylation, its interactions with integrin alphavbeta3 may play a critical role in cell signaling in physiologic and pathologic states.
authors.affiliation: Vanderbilt Medical Center, Nashville, TN 37232, USA.
authors.forename: Corina M
authors.initials: CM
authors.lastname: Borza
authors.forename: Dorin-Bogdan
authors.initials: DB
authors.lastname: Borza
authors.forename: Vadim
authors.initials: V
authors.lastname: Pedchenko
authors.forename: Moin A
authors.initials: MA
authors.lastname: Saleem
authors.forename: Peter W
authors.initials: PW
authors.lastname: Mathieson
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sado
authors.forename: Heather M
authors.initials: HM
authors.lastname: Hudson
authors.forename: Ambra
authors.initials: A
authors.lastname: Pozzi
authors.forename: Juan
authors.initials: J
authors.lastname: Saus
authors.forename: Dale R
authors.initials: DR
authors.lastname: Abrahamson
authors.forename: Roy
authors.initials: R
authors.lastname: Zent
authors.forename: Billy G
authors.initials: BG
authors.lastname: Hudson
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002448:Cell Adhesion; D024141:Collagen Type IV; D006801:Humans; D050199:Podocytes / Q000502:physiology*
chemical_list: D024141:Collagen Type IV