title: Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice.
abstract: We report a considerable number of cells in the ventricular and the subventricular zones (SVZ) of newborn mice to stain positive for the PDGF beta-receptor (PDGFRB). Many of them also stained for nestin and/or GFAP but less frequently for the neuroblast marker doublecortin and for the mitotic marker Ki-67. The SVZ of mice with nestin-Cre conditional deletion of PDGFRB expressed the receptor only on blood vessels and was devoid of any morphological abnormality. PDGFRB(-/-) neurospheres showed a higher rate of apoptosis without any significant decrease in proliferation. They demonstrated reduced capacities of migration and neuronal differentiation in response to not only PDGF-BB but also bFGF. Furthermore, the PDGFR kinase inhibitor STI571 blocked the effects of bFGF in control neurosphere cultures. bFGF increased the activity of the PDGFRB promoter as well as the expression and phosphorylation of PDGFRB. These results suggest the presence of the signaling convergence between PDGF and FGF. PDGFRB is needed for survival, and the effects of bFGF in migration and neural differentiation of the cells may be potentiated by induction of PDGFRB.
authors.affiliation: Institute of Biomedicine, Department of Medical Chemistry and Cell Biology, Göteborg University, Box 420, SE 405 30 Gothenburg, Sweden.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ishii
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Rie
authors.initials: R
authors.lastname: Watanabe
authors.forename: Muna
authors.initials: M
authors.lastname: Elmi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Fujimori
authors.forename: Johan
authors.initials: J
authors.lastname: Nissen
authors.forename: Yihai
authors.initials: Y
authors.lastname: Cao
authors.forename: Yo-Ichi
authors.initials: Y
authors.lastname: Nabeshima
authors.forename: Masakiyo
authors.initials: M
authors.lastname: Sasahara
authors.forename: Keiko
authors.initials: K
authors.lastname: Funa
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001549:Benzamides; D001973:Bromodeoxyuridine / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D000068877:Imatinib Mesylate; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D020547:Lateral Ventricles / Q000166:cytology* / Q000254:growth & development*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin; D009474:Neurons / Q000502:physiology*; D010879:Piperazines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D014162:Transfection / Q000379:methods
chemical_list: D001549:Benzamides; D004791:Enzyme Inhibitors; D007381:Intermediate Filament Proteins; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate; D020797:Receptor, Platelet-Derived Growth Factor beta; D001973:Bromodeoxyuridine