title: Survivin overexpression alone does not alter megakaryocyte ploidy nor interfere with erythroid/megakaryocytic lineage development in transgenic mice.
abstract: The level of survivin was reported to be scarce in mouse megakaryocytes (MKs) compared with erythroid cells. Considering this finding and previously reported in vitro data showing decreased MK ploidy upon retroviral-mediated overexpression of survivin, we sought to examine whether ectopic survivin expression in the MK lineage might alter ploidy level in vivo. Here we report the generation of 2 tissue specific hematopoietic transgenic mouse models, one expressing survivin in both the erythroid and MK lineages and the other expressing survivin solely in the MK lineage. Survivin protein overexpression was confirmed in MKs and erythrocytes. Surprisingly, analysis of both transgenic mouse lines showed no detectable changes in MK number, ploidy level, and platelet and erythrocyte counts, as compared with control mice. We conclude that elevated survivin expression does not alter MK/erythroid lineage development and that elevated survivin, alone, does not interfere with MK ploidy in vivo.
authors.affiliation: Department of Biochemistry, 15 Stoughton St, K225, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Donald J
authors.initials: DJ
authors.lastname: McCrann
authors.forename: Todd
authors.initials: T
authors.lastname: Yezefski
authors.forename: Hao G
authors.initials: HG
authors.lastname: Nguyen
authors.forename: Nicholas
authors.initials: N
authors.lastname: Papadantonakis
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wen
authors.forename: John D
authors.initials: JD
authors.lastname: Crispino
authors.forename: Katya
authors.initials: K
authors.lastname: Ravid
mesh_terms: D000818:Animals; D002452:Cell Count; D019070:Cell Lineage; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D050982:GATA1 Transcription Factor / Q000378:metabolism; D005786:Gene Expression Regulation; D006400:Hematocrit; D051034:Inhibitor of Apoptosis Proteins; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000235:genetics; D011003:Ploidies; D012097:Repressor Proteins; D000077022:Survivin; D019076:Transgenes
chemical_list: C117749:Birc5 protein, mouse; D050982:GATA1 Transcription Factor; C494689:Gata1 protein, mouse; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D012097:Repressor Proteins; D000077022:Survivin; D010978:Platelet Factor 4