title: Structure and estrogenic activity of new lignans from Iryanthera lancifolia.
abstract: Five new dibenzylbutane type lignans (1-5) were isolated from the stem bark of Iryanthera lancifolia. Their structures were determined by extensive 1D and 2D NMR spectroscopic studies and chemical evidence. Seventeen of the isolated compounds were tested for their estrogenic activities in the estrogen responsive human breast cancer cell line MCF-7 BUS using the E-Screen proliferation assay. Cell proliferation was evaluated by the SRB assay to calculate the estrogenic parameters. The majority of the compounds induced a mitogenic response. This effect, given as Relative Proliferative Effect (RPE) to reference estrogen 17beta-estradiol (E(2)), ranged between 14% and 84%.
authors.affiliation: Instituto Universitario de Bio-Orgánica Antonio González, Avda. Astrofísico Fco, Sánchez 2, 38206 La Laguna, Tenerife, Spain.
authors.forename: Dulce
authors.initials: D
authors.lastname: Mesa-Siverio
authors.forename: Rubén P
authors.initials: RP
authors.lastname: Machín
authors.forename: Ana
authors.initials: A
authors.lastname: Estévez-Braun
authors.forename: Angel G
authors.initials: AG
authors.lastname: Ravelo
authors.forename: Olga
authors.initials: O
authors.lastname: Lock
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D048789:Phytoestrogens / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D017705:Lignans; D048789:Phytoestrogens