Measuring protein interactions by optical biosensors.
In recent years, optical evanescent wave biosensors have been used to characterize protein-protein interactions, including determination of equilibrium binding constants and bimolecular rate constants. This surface binding technique can provide information about chemical on-rate constants, the lifetimes of complexes formed, and the time course of the signal. This unit provides a thorough, well-illustrated discussion of the principles of optical biosensors, experimental design, ligand immobilization, experimental data analysis, and common obstacles and possible solutions.
Schuck
Peter
P
National Institutes of Health, Bethesda, Maryland, USA.
Boyd
Lisa F
LF
Andersen
Peter S
PS
D000906:Antibodies / Q000737:chemistry / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D015374:Biosensing Techniques / Q000379:methods*; D007118:Immunoassay / Q000379:methods; D047468:Immunoprecipitation; D007700:Kinetics; D008954:Models, Biological; D008962:Models, Theoretical; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism* / Q000493:pharmacokinetics
D000906:Antibodies; D011506:Proteins