SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway.
While severe acute respiratory syndrome coronavirus (SARS-CoV) was initially thought to enter cells through direct fusion with the plasma membrane, more recent evidence suggests that virus entry may also involve endocytosis. We have found that SARS-CoV enters cells via pH- and receptor-dependent endocytosis. Treatment of cells with either SARS-CoV spike protein or spike-bearing pseudoviruses resulted in the translocation of angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV, from the cell surface to endosomes. In addition, the spike-bearing pseudoviruses and early endosome antigen 1 were found to colocalize in endosomes. Further analyses using specific endocytic pathway inhibitors and dominant-negative Eps15 as well as caveolin-1 colocalization study suggested that virus entry was mediated by a clathrin- and caveolae-independent mechanism. Moreover, cholesterol- and sphingolipid-rich lipid raft microdomains in the plasma membrane, which have been shown to act as platforms for many physiological signaling pathways, were shown to be involved in virus entry. Endocytic entry of SARS-CoV may expand the cellular range of SARS-CoV infection, and our findings here contribute to the understanding of SARS-CoV pathogenesis, providing new information for anti-viral drug research.
Wang
Hongliang
H
National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College, Tsinghua University and Chinese Academy of Medical Sciences, Beijing 100005, China.
Yang
Peng
P
Liu
Kangtai
K
Guo
Feng
F
Zhang
Yanli
Y
Zhang
Gongyi
G
Jiang
Chengyu
C
D048868:Adaptor Proteins, Signal Transducing; D000085962:Angiotensin-Converting Enzyme 2; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D021941:Caveolae / Q000378:metabolism / Q000821:virology; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002966:Clathrin / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis / Q000235:genetics; D011992:Endosomes / Q000378:metabolism* / Q000821:virology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D045169:Severe Acute Respiratory Syndrome / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
D048868:Adaptor Proteins, Signal Transducing; C495540:CAV1 protein, human; D002135:Calcium-Binding Proteins; D051242:Caveolin 1; D002966:Clathrin; C486206:EPS15 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D010750:Phosphoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; D007703:Peptidyl-Dipeptidase A; C000705307:ACE2 protein, human; D000085962:Angiotensin-Converting Enzyme 2