title: Localization in stereocilia, plasma membrane, and mitochondria suggests diverse roles for NMHC-IIa within cochlear hair cells.
abstract: NMHC-IIa, a nonmuscle myosin heavy chain isoform encoded by MYH9, is expressed in sensory hair cells and its dysfunction is associated with syndromic and nonsyndromic hearing loss. In this study, we investigate the ultrastructural distribution of NMHC-IIa within murine hair cells to elucidate its potential role in hair cell function. Using previously characterized anti-mouse NMHC-IIa antibody detected with immunogold labelling, NMHC-IIa was observed in the stereocilia, in the cytosol along the plasma membrane, and within mitochondria. Within stereocilia, presence of NMHC-IIa is observed throughout its length along the actin core, from the center to the periphery and at its base in the cuticular plate, suggesting a potential role in structural support. Within the sensory hair cells, NMHC-IIa was distributed throughout the cytoplasm and along the plasma membrane. A novel finding of this study is the localization of NMHC-IIa within the mitochondria, with the majority of the label along its inner membrane folds. The presence of NMHC-IIa within heterogeneous areas of the hair cell suggests that it may play different functional roles in these distinct regions. Thus, mutant NMHC-IIa may cause hearing loss by affecting hair cell dysfunction through structural and or functional disruption of its stereocilia, plasma membrane, and/or mitochondria.
authors.affiliation: Laboratory of Molecular Otology, Department of Otolaryngology, New York University School of Medicine, New York, NY 10016, USA.anil.lalwani@nyumc.org
authors.forename: Anil K
authors.initials: AK
authors.lastname: Lalwani
authors.forename: Graham
authors.initials: G
authors.lastname: Atkin
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jennifer Y
authors.initials: JY
authors.lastname: Lee
authors.forename: Dean E
authors.initials: DE
authors.lastname: Hillman
authors.forename: Anand N
authors.initials: AN
authors.lastname: Mhatre
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D015870:Gene Expression; D006198:Hair Cells, Auditory / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D018995:Myosin Heavy Chains; D024507:Nonmuscle Myosin Type IIA / Q000096:biosynthesis*
chemical_list: C525088:Myh9 protein, mouse; D024507:Nonmuscle Myosin Type IIA; D018995:Myosin Heavy Chains