A systematic library for comprehensive overexpression screens in Saccharomyces cerevisiae.
Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.
Jones
Grace Marie
GM
Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
Stalker
Jim
J
Humphray
Sean
S
West
Anthony
A
Cox
Tony
T
Rogers
Jane
J
Dunham
Ian
I
Prelich
Gregory
G
D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*