Effects of interleukin-6, leukemia inhibitory factor, and ciliary neurotrophic factor on the proliferation and differentiation of adult human myoblasts.
Our previous studies have demonstrated that ciliary neurotrophic factor, a member of the interleukin-6-type cytokine superfamily, could inhibit the differentiation of myoblasts into mature myotubes at a certain concentration. In this study, another two members, interleukin-6 and leukemia inhibitory factor, together with ciliary neurotrophic factor were tested their roles in the proliferation and differentiation of myoblasts derived from the adult human skeletal muscles, in order to confirm that these cytokines might be a new type of regulatory factors on the myoblasts. The results showed that the effects of interleukin-6, leukemia inhibitory factor, and ciliary neurotrophic factor on the proliferation and differentiation of adult human myoblasts were different. Leukemia inhibitory factor in the dose of 10 ng/ml could accelerate the cell proliferation. Leukemia inhibitory factor in the dose of 10 or 50 ng/ml and ciliary neurotrophic factor in the dose of 10 or 50 ng/ml could inhibit the myoblast differentiation. The inhibition mechanism might be that leukemia inhibitory factor and ciliary neurotrophic factor inhibited the expressions of transcription factor MyoD/myf5, which could regulate the myoblast differentiation. This study will provide the experimental and theoretic foundations for the basic and clinical researches about human myoblasts.
Wang
Xuan
X
Department of Physiology, Capital Medical University, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, 10# You An Men, Fengtai District, Beijing, 100069, P.R. China.
Wu
Haitao
H
Zhang
Zhenxing
Z
Liu
Shuhong
S
Yang
Jian
J
Chen
Xiaoping
X
Fan
Ming
M
Wang
Xiaomin
X
D000328:Adult; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology*; D002999:Clone Cells; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology*; D008561:Membrane Fusion / Q000187:drug effects; D017570:MyoD Protein / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5 / Q000378:metabolism; D018008:Myogenin / Q000378:metabolism; D009218:Myosins / Q000378:metabolism
D020934:Ciliary Neurotrophic Factor; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C497417:MYF5 protein, human; D017570:MyoD Protein; D051787:Myogenic Regulatory Factor 5; D018008:Myogenin; D009218:Myosins