title: Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler.
abstract: BACKGROUND
  5382insC BRCA1 frameshift mutation is a common founder mutation for many populations worldwide and a high-risk allele for the development of hereditary breast and/or ovarian cancer. Our goal was to develop a novel, reliable and rapid method for its detection.
  
  
  METHODS
  We developed an asymmetric real-time PCR method with hybridization probes in the LightCycler. Genotyping was performed by melting curve analysis.
  
  
  CONCLUSIONS
  The developed method was in concordance with reference methods when tested in 85 peripheral blood and 107 tumor DNA samples from Greek breast and/or ovarian cancer patients. The described method proved to be simple, cost-effective, easy to perform and rapid enough for routine use as a screening method in high-risk families and especially in the Greek, Slavic and Jewish populations where 5382insC mutation is the most common BRCA1 mutation.
authors.affiliation: Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece.
authors.forename: Christos
authors.initials: C
authors.lastname: Kroupis
authors.forename: Kostantinos
authors.initials: K
authors.lastname: Christopoulos
authors.forename: Myrto
authors.initials: M
authors.lastname: Devetzoglou
authors.forename: Ioannis
authors.initials: I
authors.lastname: Tsiagas
authors.forename: Evi S
authors.initials: ES
authors.lastname: Lianidou
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics; D017931:DNA Primers; D005260:Female; D006801:Humans; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D019313:BRCA1 Protein; D017931:DNA Primers