Antitumor effect of all-trans retinoic acid-encapsulated nanoparticles of methoxy poly(ethylene glycol)-conjugated chitosan against CT-26 colon carcinoma in vitro.
All-trans retinoic acid (ATRA)-incorporated nanoparticles of methoxy poly(ethylene glycol) (MPEG)-grafted chitosan were prepared through ion-complex formation between ATRA and chitosan. This nanoparticle has around 100 nm of diameter and favorable reconstitution properties. ATRA-incorporated nanoparticles has almost similar cytotoxicity against CT-26 tumor cells when compared to free ATRA. But nanoparticles was more effective to inhibit invasion of tumor cells than free ATRA at invasion test using matrigel. These results can be explained by apoptosis analysis using flow cytometer. When free ATRA or ATRA-incorporated nanoparticles were treated, tumor cells were slight progressed apoptosis. Furthermore, apoptosis was also progressed by treated with MPEG-grafted chitosan.
Park
Jung-Sun
JS
Cancer Vaccine Team, Chonnam National University Hwasun Hospital, Jeonnam, South Korea. sun00719@hotmail.com
Koh
Yang-Seok
YS
Bang
Je-Yong
JY
Jeong
Young-Il
YI
Lee
Je-Jung
JJ
D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006801:Humans; D053758:Nanoparticles; D011092:Polyethylene Glycols / Q000737:chemistry*; D014212:Tretinoin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D011092:Polyethylene Glycols; D014212:Tretinoin; D048271:Chitosan