A subset of mucosa-associated Escherichia coli isolates from patients with colon cancer, but not Crohn's disease, share pathogenicity islands with urinary pathogenic E. coli.
Adherent and invasive mucosa-associated Escherichia coli have been implicated in the pathogenesis of colon cancer and inflammatory bowel diseases. It has been reported that such isolates share features of extraintestinal E. coli (ExPEC) and particularly uropathogenic E. coli (UPEC). We used suppression subtractive hybridization (SSH) to subtract the genome of E. coli K-12 from that of a colon cancer mucosal E. coli isolate. Of the subtracted sequences, 53 % were present in the genomes of one or more of three sequenced UPEC strains but absent from the genome of an enterohaemorrhagic E. coli (EHEC) strain. Of the subtracted sequences, 80 % matched at least one UPEC genome, whereas only 4 % were absent from the UPEC genomes but present in the genome of the EHEC strain. A further genomic subtraction against the UPEC strain 536 enriched for sequences matching mobile genetic elements, other ExPEC strains, and other UPEC strains or commensals, rather than strains associated with gastrointestinal disease. We analysed the distribution of selected subtracted sequences and UPEC-associated pathogenicity islands (PAIs) amongst a panel of mucosa-associated E. coli isolated from colonoscopic biopsies of patients with colon cancer, patients with Crohn's disease and controls. This enabled us to identify a group of isolates from colon cancer (30-40 %) carrying multiple genes previously categorized as UPEC-specific and implicated in virulence.
Bronowski
Christina
C
Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
Smith
Shirley L
SL
Division of Gastroenterology, School of Clinical Science, University of Liverpool, Crown Street, Liverpool L69 3BX, UK.|Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
Yokota
Kyoko
K
Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
Corkill
John E
JE
Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
Martin
Helen M
HM
Division of Gastroenterology, School of Clinical Science, University of Liverpool, Crown Street, Liverpool L69 3BX, UK.
Campbell
Barry J
BJ
Division of Gastroenterology, School of Clinical Science, University of Liverpool, Crown Street, Liverpool L69 3BX, UK.
Rhodes
Jonathan M
JM
Division of Gastroenterology, School of Clinical Science, University of Liverpool, Crown Street, Liverpool L69 3BX, UK.
Hart
C Anthony
CA
Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
Winstanley
Craig
C
Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Daulby Street, Liverpool L69 3GA, UK.
D003110:Colonic Neoplasms / Q000382:microbiology*; D003424:Crohn Disease / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D044404:Genomic Islands; D006801:Humans; D008969:Molecular Sequence Data; D009092:Mucous Membrane / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014552:Urinary Tract Infections / Q000382:microbiology*
D004269:DNA, Bacterial