Anoxic preconditioning: a way to enhance the cardioprotection of mesenchymal stem cells.
OBJECTIVE
We hypothesized that anoxic preconditioning (AP) could enhance the cardioprotective effect of mesenchymal stem cells (MSCs).


METHODS
Myocardial infarction (MI) was set up in Sprague-Dawley rats and left ventricles were randomly injected with the following: DMEM, MSCs and AP-MSCs. Cardiac function was assessed by echocardiography 4 weeks after transplantation, hematoxylin-eosin staining and Masson trichrome were performed subsequently.


RESULTS
Increased fractional shortening, ejection fraction and decreased infarct size were observed most obviously in AP-MSCs group, accompanied by increased arteriole density and cell survival.


CONCLUSIONS
AP enhanced the capacity of MSCs to repair infarcted myocardium, attributable to increased cell survival and angiogenesis.
Wang
Jian-an
JA
He
Aina
A
Hu
Xinyang
X
Jiang
Yun
Y
Sun
Yong
Y
Jiang
Jun
J
Gui
Chun
C
Wang
Yaping
Y
Chen
Han
H
D000818:Animals; D000860:Hypoxia / Q000503:physiopathology; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology; D009203:Myocardial Infarction / Q000503:physiopathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley