[Proteomics of follicular fluid in mature human follicles and antral follicles: a comparative study with laser desorption/ionization-time of flight-mass spectrometry].
OBJECTIVE
To study the differentially expressed proteins between mature and antral follicles and identify the proteins crucial for follicle development and oocyte fertilization.


METHODS
Mature follicular fluids were obtained from 48 women after oocyte collection during in vitro fertilization (IVF), and antral follicular fluids were collected from 21 women by follicular puncture. The proteins in the follicular fluids were detected by surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) and weak cation-exchange protein chip (WCX-2). The data were read with PBSII-C type protein chip reader and analyzed with Biomarker Wizard and Biomarker Patterns Software of Ciphergen Company.


RESULTS
In comparison with those in the antral follicular fluid, the concentration of 4 proteins were significantly different in mature follicular fluid, including two up-regulated and two down-regulated proteins.


CONCLUSIONS
Significant variation occurs in the proteomics of mature follicular fluid, and the differentially expressed proteins may have close relation to follicle development and oocyte fertilization.
Li
Li
L
Department of Obstetrics and Gynecology, Kunming General Hospital of Chengdu Command, Kunming 650032, China. wangsanbinlili@163.net
Xing
Fu-qi
FQ
Chen
Shi-ling
SL
Sun
Ling
L
Li
Hong
H
D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D006080:Ovarian Follicle / Q000378:metabolism*; D040081:Protein Array Analysis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
D020543:Proteome