title: [Mitochondrial DNA A1555G mutation analysis in 802 nonsyndromic hearing impairment patients].
abstract: OBJECTIVE
  To investigate the prevalence of the mitochondrial DNA (mtDNA) A1555G mutation in nonsyndromic hearing impairment (NSHI) patients from Gansu province.
  
  
  METHODS
  Subjects included 802 students selected from five Deaf-Mute Schools in Gansu. DNA was extracted from peripheral blood of all patients. The mitochondrial DNA target fragments were amplified by polymerase chain reaction (PCR). The Mutations were detected by AIw26I digestion and sequence analysis.
  
  
  RESULTS
  The homoplasmic A1555G mutation was found in 67 individuals from 802 patients (8.4%). Fifteen of these 67 patients had family histories.
  
  
  CONCLUSIONS
  The mtDNA A1555G mutation had a higher incidence in Gansu population with nonsyndromic hearing impairment than other studies. The data not only gaven more evidences that the prevalence of mtDNA A1555G mutation in china was higher than that in Europe and America, but also gaven valuable information for gene diagnosis, genetic counseling and would improve the safety of aminoglycoside antibiotic therapy.
authors.affiliation: Department of Otorhinolaryngology Head Neck Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, China.
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Liu
authors.forename: Yu-fen
authors.initials: YF
authors.lastname: Guo
authors.forename: Dong-yi
authors.initials: DY
authors.lastname: Han
authors.forename: Ya-li
authors.initials: YL
authors.lastname: Zhao
authors.forename: Lan
authors.initials: L
authors.lastname: Lan
authors.forename: Cui
authors.initials: C
authors.lastname: Zhao
authors.forename: Qiu-ju
authors.initials: QJ
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D004272:DNA, Mitochondrial / Q000235:genetics*; D003638:Deafness / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009154:Mutation; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial