Preparation of mannan modified anionic PCL-PEG-PCL nanoparticles at one-step for bFGF antigen delivery to improve humoral immunity.
In this article, blank anionic poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCEC) and anionic mannan modified PCEC (MPCEC) nanoparticles with nearly the same particle size and zeta potential were prepared by emulsion solvent evaporation method. Human basic fibroblast growth factor (bFGF) was absorbed onto anionic nanoparticles surface due to electrostatic interaction. The obtained bFGF-nanoparticles complexes were injected subcutaneously into C57BL/6 mice at 20 microg of bFGF/dose on week 0, 1, 2 and 3. The mice serum was collected on week 4, and bFGF-specific autoantibody total IgG, IgG1 and IgG2a titer in serum was determined by ELISA. The results indicated that the autoantibody IgG, IgG1 and IgG2a titer of the mice immunized by bFGF-MPCEC complexes were higher than that immunized by either bFGF-PCEC or bFGF-Alum. This phenomenon might be due to that mannan functionalized MPCEC nanoparticles could be targeted to dendritic cells (DCs) to improve humoral immunity. The prepared anionic mannan modified PCEC nanoparticles (MPCEC) might have great potential application in vaccine delivery systems.
Gou
Maling
M
State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Dai
Mei
M
Li
XingYi
X
Yang
Li
L
Huang
MeiJuan
M
Wang
YongShen
Y
Kan
Bing
B
Lu
You
Y
Wei
YuQuan
Y
Qian
ZhiYong
Z
D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D016503:Drug Delivery Systems; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007279:Injections, Subcutaneous; D008351:Mannans; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D011091:Polyesters / Q000138:chemical synthesis; D011092:Polyethylene Glycols / Q000138:chemical synthesis
D000941:Antigens; D007074:Immunoglobulin G; D008351:Mannans; D011091:Polyesters; C515340:poly(epsilon-caprolactone)-b-poly(ethyleneglycol)-b-poly(epsilon-caprolactone); D016222:Fibroblast Growth Factor 2; D011092:Polyethylene Glycols