Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media.
The enzymatic saccharification of a model cellulosic substrate, Avicel PH-101, using an ionic liquid (IL), 1-ethyl-3-methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer (pH 5.0), cellulase was directly added to the aqueous-IL mixture at 40 degrees C. When the volume of IL to water was greater than 3:2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1:4 (v/v) resulted in over 70% of the starting amount of cellulose (10 mg/ml) being converted to glucose and cellobiose.
Kamiya
Noriho
N
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan. noritcm@mbox.nc.kyushu-u.ac.jp
Matsushita
Yuichi
Y
Hanaki
Misa
M
Nakashima
Kazunori
K
Narita
Mamiko
M
Goto
Masahiro
M
Takahashi
Haruo
H
D002475:Cellobiose / Q000737:chemistry; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004789:Enzyme Activation; D005947:Glucose / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*
D007093:Imidazoles; D052578:Ionic Liquids; D002475:Cellobiose; D002482:Cellulose; D002480:Cellulase; D005947:Glucose; C109691:microcrystalline cellulose; C518739:1-ethyl-3-methylimidazolium