A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis.
The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.
Kenefic
L J
LJ
Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
Beaudry
J
J
Trim
C
C
Huynh
L
L
Zanecki
S
S
Matthews
M
M
Schupp
J
J
Van Ert
M
M
Keim
P
P
D000483:Alleles; D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D019075:Electrophoresis, Capillary; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid
D004269:DNA, Bacterial