title: Simple HPLC-UV determination of nucleosides and its application to the authentication of Cordyceps and its allies.
abstract: A simple HPLC-UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C(18) column by isocratic elution with acetonitrile-water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine in Cordyceps were 0.28-14.15, 0.006-6.36, 0.01-0.14, 0.68-14.79 and 0.19-20.29 mg/g, respectively. Among the nucleosides studied, cordycepin was characteristically included in Cordyceps militaris (L.) Link. (CM), which is one of key Cordyceps allies, and might be a good marker for authenticating CM. The ratio of nucleosides to adenosine contents in Cordyceps seemed to be a useful marker for authentication and quality control of Cordyceps.
authors.affiliation: Division of Analytical Research for Pharmacoinformatics, Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Ikeda
authors.forename: Miho
authors.initials: M
authors.lastname: Nishimura
authors.forename: Yen
authors.initials: Y
authors.lastname: Sun
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Wada
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry*; D009705:Nucleosides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D009705:Nucleosides