Influenza detection and subtyping by reverse transcriptase polymerase chain reaction-restriction fragment length polymorphism for laboratory surveillance in Brazil.
A duplex reverse transcriptase polymerase chain reaction-restriction fragment length polymorphism for influenza virus subtyping was applied to 412 patient samples. The assay was able to discriminate all 47 influenza A H1N1 and H3N2 viruses. This rapid technique assessed if positive samples were current circulating strains or an emergent one and could be used as the 1st test in prepandemic stages.
Carraro
Emerson
E
Clinical Virology Laboratory, Infectious Diseases Unit, Medicine Department, Universidade Federal de São Paulo, São Paulo, SP 04039-032, Brazil.
Watanabe
Aripuanã Sakurada Aranha
AS
Neto
Daniel Ferreira Lima
DF
Granato
Celso Francisco Hernandes
CF
Bellei
Nancy Cristina Junqueira
NC
D001938:Brazil; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification* / Q000302:isolation & purification*; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification* / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*