title: Neonatal immunization with a single dose of recombinant BCG expressing subunit S1 from pertussis toxin induces complete protection against Bordetella pertussis intracerebral challenge.
abstract: The currently used pertussis vaccines are highly efficacious; however, neonates are susceptible to whooping cough up to the sixth month. In agreement, DTP-immunized neonate mice were not protected against intracerebral challenge with Bordetella pertussis. Neonate mice immunized with either DTP or a recombinant-BCG strain expressing the genetically detoxified S1 subunit of pertussis toxin do not show a humoral immune response against PT. On the other hand, rBCG-Pertussis induces higher PT-specific IFN-gamma production and an increase in both IFN-gamma(+) and TNF-alpha(+)-CD4(+)-T cells than the whole cell pertussis vaccine and confers protection against a lethal intracerebral challenge with B. pertussis.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, 05503-900 São Paulo, SP, Brazil.
authors.forename: Ivan P
authors.initials: IP
authors.lastname: Nascimento
authors.forename: Waldely O
authors.initials: WO
authors.lastname: Dias
authors.forename: Wagner
authors.initials: W
authors.lastname: Quintilio
authors.forename: Ana P
authors.initials: AP
authors.lastname: Christ
authors.forename: Josefina F
authors.initials: JF
authors.lastname: Moraes
authors.forename: Mary D C
authors.initials: MD
authors.lastname: Vancetto
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ribeiro-Dos-Santos
authors.forename: Isaias
authors.initials: I
authors.lastname: Raw
authors.forename: Luciana C C
authors.initials: LC
authors.lastname: Leite
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001886:Bordetella pertussis / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology; D006801:Humans; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D009163:Mycobacterium bovis / Q000235:genetics*; D037342:Pertussis Toxin / Q000235:genetics / Q000276:immunology*; D010567:Pertussis Vaccine / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014917:Whooping Cough / Q000276:immunology / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D010567:Pertussis Vaccine; D014409:Tumor Necrosis Factor-alpha; C083388:pertussis toxin, S1 subunit; D007371:Interferon-gamma; D037342:Pertussis Toxin