title: miR-125b inhibits osteoblastic differentiation by down-regulation of cell proliferation.
abstract: Although various microRNAs regulate cell differentiation and proliferation, no miRNA has been reported so far to play an important role in the regulation of osteoblast differentiation. Here we describe the role of miR-125b in osteoblastic differentiation in mouse mesenchymal stem cells, ST2, by regulating cell proliferation. The expression of miR-125b was time-dependently increased in ST2 cells, and the increase in miR-125b expression was attenuated in osteoblastic-differentiated ST2 cells induced by BMP-4. The transfection of exogenous miR-125b inhibited proliferation of ST2 cells and caused inhibition of osteoblastic differentiation. In contrast, when the endogenous miR-125b was blocked by transfection of its antisense RNA molecule, alkaline phosphatase activity after BMP-4 treatment was elevated. These results strongly suggest that miR-125b is involved in osteoblastic differentiation through the regulation of cell proliferation.
authors.affiliation: Division of Functional Genomics & Systems Medicine, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka City, Saitama 350-1241, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Ken
authors.initials: K
authors.lastname: Yagi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Tokuzawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kanesaki-Yatsuka
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Suda
authors.forename: Takenobu
authors.initials: T
authors.lastname: Katagiri
authors.forename: Toru
authors.initials: T
authors.lastname: Fukuda
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Maruyama
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuda
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Amemiya
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Kondoh
authors.forename: Hideo
authors.initials: H
authors.lastname: Tashiro
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D015536:Down-Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*
chemical_list: D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse