PATZ1 gene has a critical role in the spermatogenesis and testicular tumours.
PATZ1 is a recently discovered zinc finger protein that, due to the presence of the POZ domain, acts as a transcriptional repressor affecting the basal activity of different promoters. To gain insights into its biological role, we generated mice lacking the PATZ1 gene. Male PATZ1(-/-) mice were unfertile, suggesting a crucial role of this gene in spermatogenesis. Consistently, most of adult testes from these mice showed only few spermatocytes, associated with increased apoptosis, and complete absence of spermatids and spermatozoa, with the subsequent loss of tubular structure. The analysis of PATZ1 expression, by northern blot, western blot and immunohistochemistry, revealed its presence in Sertoli cells and, among the germ cells, exclusively in the spermatogonia. Since PATZ1 has been indicated as a potential tumour suppressor gene, we also looked at its expression in tumours deriving from testicular germ cells (TGCTs). Although expression of PATZ1 protein was increased in these tumours, it was delocalized in the cytoplasm, suggesting an impaired function. These results indicate that PATZ1 plays a crucial role in normal male gametogenesis and that its up-regulation and mis-localization could be associated to the development of TGCTs.
Fedele
M
M
IEOS, CNR and Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli 'Federico II, 80131 Naples, Italy.
Franco
R
R
Salvatore
G
G
Paronetto
M P
MP
Barbagallo
F
F
Pero
R
R
Chiariotti
L
L
Sette
C
C
Tramontano
D
D
Chieffi
G
G
Fusco
A
A
Chieffi
P
P
D000328:Adult; D000818:Animals; D017209:Apoptosis; D015152:Blotting, Northern / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051741:Kruppel-Like Transcription Factors / Q000032:analysis / Q000235:genetics*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics; D012097:Repressor Proteins / Q000032:analysis / Q000235:genetics*; D018239:Seminoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D012708:Sertoli Cells / Q000737:chemistry / Q000473:pathology; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000737:chemistry / Q000473:pathology; D013736:Testicular Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D013737:Testis / Q000737:chemistry
D051741:Kruppel-Like Transcription Factors; D009363:Neoplasm Proteins; C412784:PATZ1 protein, human; D012097:Repressor Proteins; C485875:Zfp278 protein, mouse