title: The role of prestin in the generation of electrically evoked otoacoustic emissions in mice.
abstract: Electrically evoked otoacoustic emissions are sounds emitted from the inner ear when alternating current is injected into the cochlea. Their temporal structure consists of short- and long-delay components and they have been attributed to the motile responses of the sensory-motor outer hair cells of the cochlea. The nature of these motile responses is unresolved and may depend on either somatic motility, hair bundle motility, or both. The short-delay component persists after almost complete elimination of outer hair cells. Outer hair cells are thus not the sole generators of electrically evoked otoacoustic emissions. We used prestin knockout mice, in which the motor protein prestin is absent from the lateral walls of outer hair cells, and Tecta(Delta ENT/Delta ENT) mice, in which the tectorial membrane, a structure with which the hair bundles of outer hair cells normally interact, is vestigial and completely detached from the organ of Corti. The amplitudes and delay spectra of electrically evoked otoacoustic emissions from Tecta(Delta ENT/Delta ENT) and Tecta(+/+) mice are very similar. In comparison with prestin(+/+) mice, however, the short-delay component of the emission in prestin(-/-) mice is dramatically reduced and the long-delay component is completely absent. Emissions are completely suppressed in wild-type and Tecta(Delta ENT/Delta ENT) mice at low stimulus levels, when prestin-based motility is blocked by salicylate. We conclude that near threshold, the emissions are generated by prestin-based somatic motility.
authors.affiliation: University of Sussex, School of Life Sciences, Brighton, UK.
authors.forename: Markus
authors.initials: M
authors.lastname: Drexl
authors.forename: Marcia M Mellado
authors.initials: MM
authors.lastname: Lagarde
authors.forename: Jian
authors.initials: J
authors.lastname: Zuo
authors.forename: Andrei N
authors.initials: AN
authors.lastname: Lukashkin
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Russell
mesh_terms: D000465:Algorithms; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D004558:Electric Stimulation; D005260:Female; D018072:Hair Cells, Auditory, Outer / Q000502:physiology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020409:Molecular Motor Proteins / Q000235:genetics / Q000502:physiology*; D009925:Organ of Corti / Q000502:physiology; D017084:Otoacoustic Emissions, Spontaneous / Q000502:physiology*; D012980:Sodium Salicylate / Q000494:pharmacology
chemical_list: D020409:Molecular Motor Proteins; C498720:Pres protein, mouse; D012980:Sodium Salicylate