title: Biodistribution of the radiophamarceutical sodium pertechnetate (Na99mTcO4) after massive small bowel resection in rats.
abstract: OBJECTIVE
  To evaluate the biodistribution of sodium pertecnetate (Na(99m)TcO(4)) in organs and tissues, the morphometry of remnant intestinal mucosa and ponderal evolution in rats subjected to massive resection of the small intestine.
  
  
  METHODS
  Twenty-one Wistar rats were randomly divided into three groups of 7 animals each. The short bowel (SB) group was subjected to massive resection of the small intestine; the control group (C) rats were not operated on, and soft intestinal handling was performed in sham rats. The animals were weighed weekly. On the 30th postoperative day, 0.l mL of Na(99m)TcO(4), with mean activity of 0.66 MBq was injected intravenously into the orbital plexus. After 30 minutes, the rats were killed with an overdose of anesthetic, and fragments of the liver, spleen, pancreas, stomach, duodenum, small intestine, thyroid, lung, heart, kidney, bladder, muscle, femur and brain were harvested. The biopsies were washed with 0.9% NaCl.,The radioactivity was counted using Gama Counter Wizard 1470, PerkinElmer. The percentage of radioactivity per gram of tissue (%ATI/g) was calculated. Biopsies of the remaining jejunum were analysed by HE staining to obtain mucosal thickness. Analysis of variance (ANOVA) and the Tukey test for multiple comparisons were used, considering p<0.05 as significant.
  
  
  RESULTS
  There were no significant differences in %ATI/g of the Na(99m)TcO(4) in the organs of the groups studied (p>0.05). An increase in the weight of the SB rats was observed after the second postoperative week. The jejunal mucosal thickness of the SB rats was significantly greater than that of C and sham rats (p<0.05).
  
  
  CONCLUSIONS
  In rats with experimentally-produced short bowel syndrome, an adaptive response by the intestinal mucosa reduced weight loss. The biodistribution of Na(99m)TcO(4) was not affected by massive intestinal resection, suggesting that short bowel syndrome is not the cause of misleading interpretation, if an examination using this radiopharmaceutical is indicated.
authors.affiliation: Postgraduate Program in Health Sciences, UFRN, Brazil. damasochacon@uol.com.br
authors.forename: Dâmaso de Araújo
authors.initials: Dde A
authors.lastname: Chacon
authors.forename: Irami
authors.initials: I
authors.lastname: Araújo-Filho
authors.forename: Arthur
authors.initials: A
authors.lastname: Villarim-Neto
authors.forename: Amália Cínthia Meneses
authors.initials: AC
authors.lastname: Rêgo
authors.forename: Italo Medeiros
authors.initials: IM
authors.lastname: Azevedo
authors.forename: Mário
authors.initials: M
authors.lastname: Bernardo-Filho
authors.forename: José
authors.initials: J
authors.lastname: Brandão-Neto
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007413:Intestinal Mucosa / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology / Q000601:surgery*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000378:metabolism*; D013670:Sodium Pertechnetate Tc 99m / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D019275:Radiopharmaceuticals; D013670:Sodium Pertechnetate Tc 99m