Enzymatic amplification of DNA by PCR: standard procedures and optimization.
This appendix describes a method for amplifying DNA enzymatically by the polymerase chain reaction (PCR), including procedures to quickly determine conditions for successful amplification of the sequence and primer sets of interest, and to optimize for specificity, sensitivity, and yield.
Kramer
M F
MF
Harvard Medical School, Boston, Massachusetts, USA.
Coen
D M
DM
D000818:Animals; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000592:standards; D045424:Complex Mixtures; D017931:DNA Primers; D004121:Dimethyl Sulfoxide; D006801:Humans; D007202:Indicators and Reagents; D015636:Magnesium Chloride; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D019914:Taq Polymerase; D013698:Templates, Genetic
D045424:Complex Mixtures; D017931:DNA Primers; D007202:Indicators and Reagents; D015636:Magnesium Chloride; D019914:Taq Polymerase; D004121:Dimethyl Sulfoxide