Cytotoxic activity of an octadecenoic acid extract from Euphorbia kansui (Euphorbiaceae) on human tumour cell strains.
We have investigated the cytotoxic and antitumour activity of an octadecenoic acid extract, mainly containing oleic and linoleic acids, from Euphorbia kansui on human gastric (SGC-7901), hepatocellular carcinoma (BEL-7402), and leukaemia (HL-60) tumour cell strains. Significant and dose-dependent antiproliferation effects were observed on tumour cells from the dose of 3.2 microg mL(-1), which were comparable with or better than those of the common antitumour agent 5-fluorouracil. Results from the clone formation assay and flow cytometry indicated that the mixture of octadecenoic acids resulted in a dose-dependent reduction in the number of tumour cells and significantly inhibited cell proliferation, with induced apoptosis and G(0)/G(1) phase cell cycle arrest. Also, the octadecenoic acids could not only cause cell apoptosis/necrosis but also functionally and structurally damage the tumour cell membrane and cell ultra-structures. These observations encourage further clinical evaluation of the inhibitory effects of octadecenoic acids on various forms of cancer.
Yu
Farong
F
School of Public Security, Gansu Institute of Political Science and Law, Lanzhou 730070, China. fyu@ufl.edu
Lu
Shunqing
S
Yu
Fahong
F
Shi
Junnian
J
McGuire
Peter M
PM
Wang
Rui
R
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D028481:Euphorbia / Q000737:chemistry*; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D008041:Linoleic Acids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D009829:Oleic Acids / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle / Q000187:drug effects
D000970:Antineoplastic Agents; D008041:Linoleic Acids; D009829:Oleic Acids; D010936:Plant Extracts; D005472:Fluorouracil