Liquid chromatography-tandem mass spectrometry of I3,II8-biapigenin, the major biflavone in Hypericum perforatum extracts.
High-performance liquid chromatography method coupled with tandem mass spectrometry was developed for the quantitative determination of I3,II8-biapigenin. The procedure includes solid-phase extraction and separation on an XTerra MS C18. The assay was linear over a wide range; precision and accuracy were acceptable. Biapigenin was present in mouse and rat plasma after a standardized Hypericum perforatum extract. It was not detected in brain (<5ngg(-1)), suggesting poor brain-to-blood permeability. Biapigenin concentrations were measurable in mice after intraperitoneal biapigenin (10mgkg(-1)) but these amounted to about 2% of the equivalent systemic exposure, after correction for the contribution from residual blood.
Colovic
Milena
M
Istituto di Ricerche Farmacologiche Mario Negri, via La Masa 19, 20156 Milan, Italy.
Caccia
Silvio
S
D000818:Animals; D047310:Apigenin / Q000032:analysis* / Q000493:pharmacokinetics; D044946:Biflavonoids / Q000032:analysis* / Q000493:pharmacokinetics; D001923:Brain Chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003627:Data Interpretation, Statistical; D020902:Hypericum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000032:analysis; D051381:Rats; D012015:Reference Standards; D012996:Solutions; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D053719:Tandem Mass Spectrometry
D044946:Biflavonoids; D010936:Plant Extracts; D012996:Solutions; C446502:biapigenin; D047310:Apigenin