[Preparation and anti-oxidative activity of liposomal ferulic acid].
Ferulic acid (FA) was loaded into liposomes via calcium acetate gradient with (80.2 +/- 5.2)% entrapment efficiency. The average sizes of blank liposome and FA liposome were about 155 nm and 154 nm, respectively. The zeta potential of blank liposome and FA liposome were (13.14 +/- 1.67) mV and (4.12 +/- 0.05) mV, respectively. Unilamellar vesicles were present in freeze-fracture electron microscopy. In the pharmacodynamic studies, the protective effect of liposomal ferulic acid on tBHP-challenged U937 cells was measured with the morphology of cell injury, mitochondrial transmembrane potential alternation and cell viability assay used as index. The results of MTT assay, microscopy indicated that FA liposomes exhibited greater antioxidant activity than FA solution on U937 cell.
Qin
Jing
J
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. qinjingx@sina.com
Chen
Da-wei
DW
Cui
Qiao
Q
Qiao
Ming-xi
MX
Hu
Hai-yang
HY
Zhao
Xiu-li
XL
Wang
Wei
W
D000975:Antioxidants / Q000008:administration & dosage* / Q000494:pharmacology; D002784:Cholesterol / Q000737:chemistry; D003373:Coumaric Acids / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000502:physiology; D010316:Particle Size; D020298:U937 Cells
D000975:Antioxidants; D003373:Coumaric Acids; D004337:Drug Carriers; D008081:Liposomes; D002784:Cholesterol; C004999:ferulic acid