title: Effects of triploidy after intracytoplasmic sperm injection on in vitro fertilization cycle outcome.
abstract: OBJECTIVE
  To evaluate the impact on the rates of clinical pregnancy and live birth of polyploidy after intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  University-based IVF center.
  
  
  METHODS
  One hundred forty-three patients undergoing their first IVF-embryo transfer cycle requiring ICSI.
  
  
  METHODS
  None.
  
  
  METHODS
  Patients were divided into two groups on the basis of the proportion of post-ICSI triploid fertilization that was observed at the time of fertilization assessment: group 1 included patients with <or=20% three-pronuclear (3PN) zygotes, and group 2 included patients with >20% 3PN zygotes. The primary outcomes compared between groups were pregnancy and implantation rates; secondary outcome measures included clinical-pregnancy rate and live-birth rate per embryo transfer.
  
  
  RESULTS
  Pregnancy, implantation, clinical-pregnancy, and live-birth rates were significantly higher in the cohort of patients who had <or=20% of embryos appearing triploid, compared with the group who had >20% of zygotes appearing triploid (relative risk [RR] for pregnancy, 2.4 [95% confidence interval {CI}, 1.22-4.77]; RR for implantation, 2.6 [95% CI, 1.17-5.56]; RR for clinical pregnancy, 2.8 [95% CI, 1.16-6.85]; and RR for live birth, 2.6 [95% CI, 1.06-6.38]). The proportion of 3PN zygotes after ICSI had a statistically significant inverse relationship to clinical-pregnancy rate.
  
  
  CONCLUSIONS
  The proportion of triploid zygotes after ICSI serves as a negative prognostic indicator for IVF cycle outcome. This finding suggests that triploidy proportion is a surrogate marker of oocyte competence that represents the integrity of the oocytes in the entire recruited cohort. Such findings therefore may influence recommendations for embryo transfer number and freezing of supernumerary embryos.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, The George Washington University, Washington, District of Columbia 20037, USA. mdayal@mfa.gwu.edu
authors.forename: Molina B
authors.initials: MB
authors.lastname: Dayal
authors.forename: Paul R
authors.initials: PR
authors.lastname: Gindoff
authors.forename: Abbaa
authors.initials: A
authors.lastname: Sarhan
authors.forename: Anil
authors.initials: A
authors.lastname: Dubey
authors.forename: Douglas
authors.initials: D
authors.lastname: Peak
authors.forename: David
authors.initials: D
authors.lastname: Frankfurter
mesh_terms: D010064:Embryo Implantation / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods* / Q000592:standards; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011123:Polyploidy; D011247:Pregnancy; D011256:Pregnancy Outcome; D011272:Pregnancy, Multiple / Q000706:statistics & numerical data; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods* / Q000592:standards; D014427:Twins