[Comparative proteomics of the serum in patients with nasopharyngeal carcinoma: a study with two-dimensional electrophoresis and MALDI-TOF-MS].
OBJECTIVE
To screen the tumor specific antigens of nasopharyngeal carcinoma (NPC) in the serum of the patients.


METHODS
Two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was employed to screen the specific biomarkers of NPC in the sera from 42 healthy volunteers, 37 NPC patients with lymph node metastasis and 27 NPC patients without lymphatic metastasis.


RESULTS
After pretreatment including albumin and immunoglobulin (IgG) depletion and desalting, the sera were subjected to 2-DE and image analysis. The differentially expressed protein spots were identified by MALDI-TOF-MS. Sera pretreatment resulted in better repeatability and resolution on the reference gel because of albumin and IgG depletion. From optimized 2-DE gel images, 29 spots indicating differential protein expression were identified by MALDI-TOF-MS to represent 23 proteins. Transferrin, ZNF544 protein, transthyretin, FAD-synthetase and NM23-H1 proteins were down-regulated and 12-lipoxygenase, serum amyloid A1 protein precursor, cytochrome P450, sICAM-1, cathepsin G and lysine-specific histone demethylase 1 were upregulated in the two groups of NPC patients as compared with the healthy group. Increased expressions of 12-lipoxygenase, sICAM-1, cathepsin G and lysine-specific histone demethylase 1 were detected in NPC patients with lymph node metastasis as compared with the patients without lymph node metastasis, but heat shock protein 70 was expressed only in NPC patients with lymph node metastasis.


CONCLUSIONS
The 2-DE-based serum proteome analysis can be useful in detecting the protein expression alterations due to carcinogenesis and development of NPC, and the newly discovered biomarkers might help in the diagnosis of NPC.
Liao
Qiu-Lin
QL
Department of Pathology, Southern Medical University/Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, China. lin_success@tom.com.cn
Chen
Xiao-Dong
XD
Zhao
Liang
L
Ding
Yan-Qing
YQ
D000328:Adult; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome