A novel small antifungal peptide from Bacillus strain B-TL2 isolated from tobacco stems.
A novel small antifungal peptide produced by a Bacillus strain B-TL2 isolated from tobacco stems was purified. The purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on CM-Sepharose Fast Flow column and reverse-phase HPLC on SOURCE 5RPC column. After the final isolation step, one peptide with antifungal activity, designated as BTL, was obtained. The molecular mass of the purified BTL was determined as 2500 Da and 2237.7 Da by SDS-PAGE and matrix-assisted laser desorption/ionization time of flight mass spectrometry, respectively. The N-amino acid sequence of BTL was determined to be NH(2)-KQQLATEAESAGPIL, which shows relatively low identity to other antimicrobial peptides from bacteria. The peptide exhibited strong inhibitory activity against mycelial growth of Bipolaris maydis, Alternaria brassicae, Aspergillus niger, Cercospora personata. The purified BTL displayed thermostability, almost retaining 100% activity at 100 degrees C for 15 min.
Zhang
Beibei
B
Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture of China and Biotechnology Research Center, Southwest University, Chongqing, China.
Xie
Chengjian
C
Yang
Xingyong
X
D000528:Alternaria / Q000187:drug effects / Q000254:growth & development; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001234:Aspergillus niger / Q000187:drug effects / Q000254:growth & development; D001407:Bacillus / Q000378:metabolism*; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D018547:Plant Stems / Q000382:microbiology*; D014026:Nicotiana / Q000382:microbiology*
D000935:Antifungal Agents; D010455:Peptides