[Effects of glucocorticoid and histamine on MUC5AC mRNA and protein expression in human nasal polyps].
OBJECTIVE
To study the effects of dexamethasone and histamine on the expression of MUC5AC mRNA and protein in human nasal polyps.


METHODS
All samples were randomly divided into control group, histamine stimulating group and dexamethasone group. The expression of MUC5AC mRNA and MUC5AC protein in nasal polyps were respectively detected by the methods of reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assay.


RESULTS
The expression of MUC5AC mRNA (0.6389 +/- 0.0526) and protein (0.1934 +/- 0.0137) in histamine stimulating group were significantly higher than those of control group (0.3495 +/- 0.0357 and 0.1172 +/- 0.0173, respectively) and reduced (0.4988 +/- 0.0603 and 0.1444 +/- 0.0075, respectively) after treatment with dexamethasone.


CONCLUSIONS
Histamine upregulates MUC5AC mRNA and protein expression. Dexamethasone downregulates MUC5AC mRNA and protein expression, which may be one of the mechanisms of downregulating mucus overproduction by dexamethasone in nasal polyps.
Peng
Lu
L
Department of Otolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
Zhen
Hongtao
H
Long
Xiaobo
X
Zhang
Hanfu
H
Jin
Si
S
D000293:Adolescent; D000328:Adult; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055271:Mucin 5AC / Q000235:genetics / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D009298:Nasal Polyps / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation; D055815:Young Adult
D005938:Glucocorticoids; C524678:MUC5AC protein, human; D055271:Mucin 5AC; D012333:RNA, Messenger; D003907:Dexamethasone; D006632:Histamine