Age-related reduction in retinal deimination levels in the F344BN rat.
Increased deimination and peptidyl arginine deiminase type 2 (PAD2) expression has been observed in age-related neurodegenerative diseases without discrimination between their aging and disease component. Here, we describe reduced levels of deimination commensurate with reduced protein, mRNA and activity of peptidylarginine deiminase type 2 in the retina, optic nerve and plasma of aged rats when compared to young rats. The decrease was significant in the ganglion cell layer, inner plexiform layer and inner nuclear layer. Because our observations suggest reduced deimination is a consequence of aging, we conclude that increased deimination must be a consequence of disease. Our findings are important to understand late-onset and progressive diseases such as glaucoma, pseudoexfoliation syndrome, age-related macular degeneration and Oguchi's disease.
Bhattacharya
Sanjoy K
SK
Bascom Palmer Eye Institute, 1638 NW 10th Avenue, 706, University of Miami, Miami, FL 33136, USA. sbhattacharya@med.miami.edu
Sinicrope
Brent
B
Rayborn
Mary E
ME
Hollyfield
Joe G
JG
Bonilha
Vera L
VL
D000375:Aging / Q000502:physiology*; D000818:Animals; D006867:Hydrolases / Q000235:genetics / Q000378:metabolism*; D009900:Optic Nerve / Q000502:physiology*; D000080064:Protein-Arginine Deiminase Type 2; D000076342:Protein-Arginine Deiminases; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D012160:Retina / Q000166:cytology / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000502:physiology*
D012333:RNA, Messenger; D006867:Hydrolases; C461539:Padi2 protein, rat; D000080064:Protein-Arginine Deiminase Type 2; D000076342:Protein-Arginine Deiminases