[Study on the serological diagnosis of Epstein-Barr virus among patients with mononucleosis].
OBJECTIVE
To explore the relationship of Epstein-Barr virus (EBV) specific antibodies detection and the diagnoses of infectious mononucleosis (IM) caused by EBV.


METHODS
Single serum samples were collected from 220 inpatients with the diagnosis of IM between January 2005 and December 2006 in Beijing Children's hospital. The samples were detected for EBV-CA-IgM, EBV-CA-IgG, EBV-EA-IgG, EBV-NA-IgG and the avidity of EBV-CA-IgG by indirect immunofluorescent assay.


RESULTS
The positive rate of EBV-CA-IgG, EBV-CA-IgM, EBV-EA-IgG and EBV-NA-IgG were 100%, 95.9%, 79.5% and 4.1% respectively. Low-avidity EBV-CA-IgG was detected in 204 patients (92.7%) with positive EBV-CA-IgM and EBV-CA-IgG,negative EBV-NA-IgG, low-avidity EBV-CA-IgG were the main pattern of EBV antibody in IM patients (84.6%).


CONCLUSIONS
The existence of EBV specific antibody (CA-IgG, CA-IgM, EA-IgG, NA-IgG and avidity of CA-IgG) could add more information to identify the stage of EBV infection so as to provide more reliable serological evidence for the diagnosis of IM.
Liu
Chun-Yan
CY
Laboratory of Virology, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
Yan
Jing
J
Liu
Ya-Yi
YY
Xie
Zheng-De
ZD
D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood; D000915:Antibody Affinity; D002648:Child; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007244:Infectious Mononucleosis / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D008297:Male
D000914:Antibodies, Viral; D007074:Immunoglobulin G