title: Effect of fish oil containing parenteral lipid emulsions on neutrophil chemotaxis and resident-macrophages' phagocytosis in rats.
abstract: OBJECTIVE
  There is scarce information about immune function and parenteral fish oil (FO). The influence of a new parenteral lipid emulsion (LE) containing fish oil (SMOF) was experimentally evaluated on neutrophils' chemotaxis and macrophages' phagocytosis.
  
  
  METHODS
  Adult male Lewis rats (n=40) were randomized into five groups; one non-surgical control and four to receive parenteral LE or saline infusion through jugular vein catheterization: SMOF (mixture of 30% medium-chain triglycerides, 30% soybean, 25% olive and 15% fish oils); MCT/LCT (physical mixture of 50% medium-chain triglycerides and 50% soybean oil); MCT/LCT/FO (80% MCT/LCT supplemented with 20% FO) and SS (saline). In the 5th experimental day and after intravenous colloidal carbon injection, blood and tissue (liver, lung and spleen) samples were collected and immunological analyses were performed.
  
  
  RESULTS
  LE didn't influence neutrophil chemotaxis. SMOF didn't influence phagocytosis (p>0.05) while MCT/LCT and MCT/LCT/FO LE increased the number of liver and lung resident macrophages that had engaged in phagocytosis compared with CO-NS and SS (p<0.05). Only MCT/LCT/FO increased the number of spleen resident macrophages that had engaged in phagocytosis (p<0.05).
  
  
  CONCLUSIONS
  LE, independently of composition, had no influence on neutrophils' chemotaxis, but showed different effect on phagocytosis by macrophages. SMOF LE had neutral effect while fish oil LE enriched with MCT/LCT LE increased resident-macrophages' phagocytosis.
authors.affiliation: University of São Paulo, School of Medicine, Department of Gastroenterology, Digestive Surgery Division, LIM 35, São Paulo, Brazil. leticia.denardi@gmail.com
authors.forename: Leticia
authors.initials: L
authors.lastname: De Nardi
authors.forename: Raquel
authors.initials: R
authors.lastname: Bellinati-Pires
authors.forename: Raquel S
authors.initials: RS
authors.lastname: Torrinhas
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Bacchi
authors.forename: Victor
authors.initials: V
authors.lastname: Arias
authors.forename: Dan L
authors.initials: DL
authors.lastname: Waitzberg
mesh_terms: D000818:Animals; D002633:Chemotaxis / Q000187:drug effects* / Q000276:immunology; D005217:Fat Emulsions, Intravenous / Q000737:chemistry / Q000494:pharmacology; D005395:Fish Oils / Q000737:chemistry / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000276:immunology; D008168:Lung / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D010288:Parenteral Nutrition; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D005217:Fat Emulsions, Intravenous; D005395:Fish Oils