Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries.
This unit describes the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides fused to either a minor or major capsid protein. Direct affinity selection uses target protein bound to a microtiter plate followed by purification of selected phage by ELISA. Alternatively, there is a bead-based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic databases for putative interacting proteins.
Kay
Brian K
BK
Argonne National Laboratory, Argonne, Illinois, USA.
Castagnoli
Luisa
L
D000818:Animals; D017104:Bacteriophage M13 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019151:Peptide Library; D025941:Protein Interaction Mapping / Q000379:methods*
D019151:Peptide Library