Effects of oseltamivir phosphate (Tamiflu) in human sera on results of microneutralization and hemagglutinin-inhibition tests for H5N2 avian influenza virus.
To determine the influence of oseltamivir phosphate (Tamiflu) on the results of microneutralization and hemagglutinin-inhibition (HI) tests in human sera with H5N2 influenza virus, ten volunteers were administered Tamiflu and blood samples were collected. In the microneutralization test, no consistent effects were observed. However, in the HI test, specimens from all volunteers taken at 4 and 7 h after drug administration showed a higher titer as compared to 0 and 24 h after administration when mammalian cells (horse, guinea pig, and human) were used. These results suggest that the administration of Tamiflu may affect the results of HI tests for H5N2 virus.
Yamazaki
Yoshinao
Y
Department of Genetic Science, Ibaraki prefectural Institute for Public Health, 993-2, Kasahara-cho, Mito, Ibaraki 310-0852, Japan. petitmadam@power.odn.ne.jp
Doy
M
M
Yamato
S
S
Kawada
Y
Y
Ogata
T
T
D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000998:Antiviral Agents / Q000097:blood*; D005260:Female; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006736:Horses; D006801:Humans; D053125:Influenza A Virus, H5N2 Subtype / Q000187:drug effects* / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D007564:Japan; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests; D053139:Oseltamivir / Q000097:blood*
D000914:Antibodies, Viral; D000998:Antiviral Agents; D053139:Oseltamivir