title: Changes in pH of peritoneal fluid associated with carbon dioxide insufflation during laparoscopic surgery in dogs.
abstract: OBJECTIVE
  To evaluate changes in pH of peritoneal fluid associated with CO2 insufflation during laparoscopy in dogs.
  
  
  METHODS
  13 client-owned dogs and 10 purpose-bred teaching dogs.
  
  
  METHODS
  Laparotomy was performed on control dogs; peritoneal fluid pH was measured at time of incision of the abdominal cavity (time 0) and 30 minutes later. Laparoscopic insufflation with CO2 was performed and routine laparoscopic procedures conducted on the teaching dogs. Insufflation pressure was limited to 12 mm Hg. Intraperitoneal fluid pH was measured by use of pH indicator paper at 4 time points. Arterial blood gas analysis was performed at the same time points.
  
  
  RESULTS
  Peritoneal fluid pH did not change significantly between 0 and 30 minutes in the control dogs. For dogs with CO2 insufflation, measurements obtained were a mean of 8.5, 24.5, 44.5, and 72.0 minutes after insufflation. The pH of peritoneal fluid decreased significantly between the first (7.825 +/- 0.350) and second (7.672 +/- 0.366) time point. Blood pH decreased significantly between the first (7.343 +/- 0.078), third (7.235 +/- 0.042), and fourth (7.225 +/- 0.038) time points. The PaCO2 increased significantly between the first (39.9 +/- 9.8 mm Hg) and fourth (54.6 +/- 4.4 mm Hg) time points. Base excess decreased significantly between the first and all subsequent time points.
  
  
  CONCLUSIONS
  Pneumoperitoneum attributable to CO2 insufflation caused a mild and transient decrease in peritoneal fluid pH in dogs. Changes in peritoneal fluid associated with CO2 insufflation in dogs were similar to those in other animals.
authors.affiliation: Department of Clinical Sciences, Veterinary Medical Center, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
authors.forename: Felix M
authors.initials: FM
authors.lastname: Duerr
authors.forename: David C
authors.initials: DC
authors.lastname: Twedt
authors.forename: Eric
authors.initials: E
authors.lastname: Monnet
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry* / Q000187:drug effects*; D002245:Carbon Dioxide / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D006863:Hydrogen-Ion Concentration; D007044:Hysterectomy / Q000662:veterinary; D007327:Insufflation / Q000662:veterinary*; D010535:Laparoscopy / Q000662:veterinary*
chemical_list: D002245:Carbon Dioxide