Prolactin and growth hormone induce differential cytokine and chemokine profile in murine peritoneal macrophages in vitro: involvement of p-38 MAP kinase, STAT3 and NF-kappaB.
The role of immune-neuroendocrine interactions in the autoimmune diseases is well recognized. Autoimmune rheumatoid diseases in their active phase have been characterized by high levels of prolactin (PRL) as well as proinflammatory cytokines which suggest a co-relationship between them. In the present study, we have investigated the profile of cytokines secreted by macrophages on treatment with PRL and growth hormone (GH) in vitro. Significantly enhanced production of cytokines IL-1beta, IL-12p40 and IFN-gamma was observed on treatment of macrophages with PRL or GH. However, higher doses of PRL (1000 ng/ml) induced the production of anti-inflammatory cytokine IL-10, with significant abrogation in production of proinflammatory cytokines. It is further observed that PRL and GH induced the production of chemokines MIP-1alpha and RANTES. PRL but not GH selectively induced significantly enhanced production of MCP-1 and IP-10. It is further shown that p38 MAP kinase, STAT3 and NF-kappaB could play a differential regulatory role in PRL or GH induced production of cytokines by macrophages.
Sodhi
Ajit
A
School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, UP 221005, India. ajit.sodhi@lycos.com
Tripathi
Anurag
A
D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis; D054405:Chemokine CCL3 / Q000096:biosynthesis; D018946:Chemokine CCL5 / Q000096:biosynthesis; D054357:Chemokine CXCL10 / Q000096:biosynthesis; D018925:Chemokines / Q000096:biosynthesis*; D003474:Curcumin / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D020672:I-kappa B Proteins / Q000096:biosynthesis; D007093:Imidazoles / Q000494:pharmacology; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D053762:Interleukin-12 Subunit p40 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000502:physiology*; D010766:Phosphorylation; D011112:Polymyxin B / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000502:physiology*; D020032:Tyrphostins / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology*
C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D054405:Chemokine CCL3; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018925:Chemokines; D016207:Cytokines; D020672:I-kappa B Proteins; D007093:Imidazoles; D053762:Interleukin-12 Subunit p40; D053583:Interleukin-1beta; D016328:NF-kappa B; D011725:Pyridines; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D016753:Interleukin-10; D007371:Interferon-gamma; D011388:Prolactin; D013006:Growth Hormone; D048051:p38 Mitogen-Activated Protein Kinases; D003474:Curcumin; D011112:Polymyxin B; C090942:4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole