title: Detection of Chronic bee paralysis virus (CBPV) genome and its replicative RNA form in various hosts and possible ways of spread.
abstract: Detection of Chronic bee paralysis virus (CBPV) is reported for the first time in two species of ants (Camponotus vagus and Formica rufa) and in Varroa destructor. A quantitative real-time PCR (qPCR) method was used to detect and quantify CBPV in infected bees, ants and mites. A minus-strand-specific RT-PCR was used to assess viral replication. These results suggest a new way by which the infection may be spread and other sites of viral persistence in the close apiary environment.
authors.affiliation: Agence Française de Sécurité Sanitaire des Aliments, Les Templiers, Route des Chappes, BP 111, Sophia Antipolis 06902, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Celle
authors.forename: Philippe
authors.initials: P
authors.lastname: Blanchard
authors.forename: Violaine
authors.initials: V
authors.lastname: Olivier
authors.forename: Frank
authors.initials: F
authors.lastname: Schurr
authors.forename: Nicolas
authors.initials: N
authors.lastname: Cougoule
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Faucon
authors.forename: Magali
authors.initials: M
authors.lastname: Ribière
mesh_terms: D000818:Animals; D001000:Ants / Q000821:virology*; D001516:Bees / Q000821:virology*; D002908:Chronic Disease; D016679:Genome, Viral; D007304:Insect Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008925:Mites / Q000821:virology*; D010243:Paralysis / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D012367:RNA, Viral