title: Evidence for GC preference by monocot Dicer-like proteins.
abstract: Dicot Dicer-like (DCL) enzymes operate preferably on GC rich regions when producing small interfering (si)RNA and micro (mi)RNA. This GC bias, however, is not generic in monocot miRNA productions. From wild Dactylis glomerata naturally infected by Cocksfoot streak potyvirus (CSV), CSV-siRNAs had a greater GC% than the virus genome, indicating that GC rich regions were also preferred by the grass DCLs. This supports the notion that GC preference is an ancient feature for plant DCLs, and suggests that monocot miRNA genes might have evolved to a high GC% resulting in GC bias being not detectable during mature miRNA production.
authors.affiliation: NERC/Centre for Ecology and Hydrology (CEH) Oxford, Mansfield Road, Oxford OX1 3SR, UK.
authors.forename: Thien
authors.initials: T
authors.lastname: Ho
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Rusholme Pilcher
authors.forename: Mary-Lou
authors.initials: ML
authors.lastname: Edwards
authors.forename: Ian
authors.initials: I
authors.lastname: Cooper
authors.forename: Tamas
authors.initials: T
authors.lastname: Dalmay
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D001482:Base Composition / Q000235:genetics*; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics*; D031745:Dactylis / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D043244:Ribonuclease III / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D034741:RNA, Small Interfering; D043244:Ribonuclease III