Influence of cyclosporine A on mucociliary system after lung transplantation in rats.
OBJECTIVE
To investigate the function of the bronchial mucociliary system in transplanted rat lungs with and without the influence of immunosuppression.


METHODS
Thirty-six rats underwent single-lung transplantation and were divided into two groups, one of which received cyclosporine treatment, and the control group which did not. Cyclosporine was administered subcutaneously in doses of 10 mg/kg daily. The rats were sacrificed 2, 15 or 30 days after transplantation. In situ bronchial mucociliary transport (MCT) and ciliary beat frequency (CBF) were determined proximal and distal to the bronchial anastomosis.


RESULTS
Significant progressive improvement on MCT, proximal and distal to the anastomotic site, was also found in the cyclosporine-treated group at 15 and 30 days (p<0.01). No significant change in MCT was found in the control group. CBF behavior in the two groups. Histological analysis showed that rejection was significantly higher in the control group (p<0.05).


CONCLUSIONS
Cyclosporine has a positive influence on bronchial mucociliary transport but not on CBF due to the effect of the rejection mechanism.
Xavier
Alexandre Martins
AM
Thoracic Surgery Division, Department of Surgery, Federal University of São Paulo (UNIFESP)São Paulo, Brazil.
Pêgo-Fernandes
Paulo Manuel
PM
Correia
Aristides Tadeu
AT
Pazetti
Rogerio
R
Monteiro
Rosangela
R
Canzian
Mauro
M
Jatene
Fabio Biscegli
FB
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D016040:Lung Transplantation; D009079:Mucociliary Clearance / Q000187:drug effects*; D051381:Rats
D007166:Immunosuppressive Agents; D016572:Cyclosporine