title: Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion.
abstract: The aim of this study was to compare the outcomes of nerve autografts (GRF) and venous grafts containing mononuclear bone marrow cells (BMCs) in sciatic nerve-lesioned rats. Control animals underwent sham operations (SHAM), received empty venous grafts (EPV), or received venous grafts containing BMC vehicle (AGR). Outcome was evaluated through sciatic functional index (SFI), morphometric and morphologic analyses of the nerve distal to the lesion, and the number of spinal cord motor neurons positive for the retrograde tracer, Fluoro-Gold. All groups exhibited poor results in SFI when compared to SHAM animals throughout the postoperative period. All groups also had a significantly greater fiber density, decreased fiber diameter, and decreased motor neuron number than the SHAM group. No significant difference between the GRF and BMC groups was observed in any of these parameters. On the other hand, vessel density was significantly higher in BMC than all other groups. BMC-containing venous grafts are superior to nerve autografts in increasing vessel density during sciatic nerve regeneration.
authors.affiliation: Department of Orthopedics and Traumatology, Federal University of São Paulo, Rua Borges Lagoa 786, São Paulo, Brazil. fernandesmarcela@click21.com
authors.forename: Marcela
authors.initials: M
authors.lastname: Fernandes
authors.forename: Sandra Gomes
authors.initials: SG
authors.lastname: Valente
authors.forename: Maria José da Silva
authors.initials: MJ
authors.lastname: Fernandes
authors.forename: Evandro Penteado Villar
authors.initials: EP
authors.lastname: Félix
authors.forename: Maria da Graça Naffah
authors.initials: Mda G
authors.lastname: Mazzacoratti
authors.forename: Débora Amado
authors.initials: DA
authors.lastname: Scerni
authors.forename: João Baptista Gomes
authors.initials: JB
authors.lastname: dos Santos
authors.forename: Vilnei Mattioli
authors.initials: VM
authors.lastname: Leite
authors.forename: Flávio
authors.initials: F
authors.lastname: Faloppa
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000637:transplantation; D001854:Bone Marrow Cells / Q000502:physiology*; D002452:Cell Count; D005456:Fluorescent Dyes; D008297:Male; D009186:Myelin Sheath / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009412:Nerve Fibers / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D012584:Sciatic Nerve / Q000293:injuries* / Q000502:physiology; D013266:Stilbamidines
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines