title: Cutting edge: CD4 T cell-mast cell interactions alter IgE receptor expression and signaling.
abstract: Mast cell activation is associated with atopic and inflammatory diseases, but the natural controls of mast cell homeostasis are poorly understood. We hypothesized that CD4(+)CD25(+) regulatory T cells (Treg) could function in mast cell homeostasis. In this study, we demonstrate that mast cells can recruit both Treg and conventional CD4(+) T cells (Tconv). Furthermore, Treg, but not Tconv, suppress mast cell FcepsilonRI expression. Despite the known inhibitory functions of IL-10 and TGFbeta1, FcepsilonRI suppression was independent of IL-10 and TGF-beta1 and required cell contact. Surprisingly, coculture with either Treg or Tconv cells suppressed IgE-mediated leukotriene C(4) production but enhanced cytokine production by mast cells. This was accompanied by a selective increase in FcepsilonRI-mediated Stat5 phosphorylation, which is a critical mediator of IgE-mediated cytokine secretion. These data are the first direct demonstration that mast cells can recruit Treg and illustrate that T cell interactions can alter the mast cell response.
authors.affiliation: Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA.
authors.forename: Mohit
authors.initials: M
authors.lastname: Kashyap
authors.forename: Angela M
authors.initials: AM
authors.lastname: Thornton
authors.forename: Sarah Kennedy
authors.initials: SK
authors.lastname: Norton
authors.forename: Brian
authors.initials: B
authors.lastname: Barnstein
authors.forename: Matthew
authors.initials: M
authors.lastname: Macey
authors.forename: Jennifer
authors.initials: J
authors.lastname: Brenzovich
authors.forename: Ethan
authors.initials: E
authors.lastname: Shevach
authors.forename: Warren J
authors.initials: WJ
authors.lastname: Leonard
authors.forename: John J
authors.initials: JJ
authors.lastname: Ryan
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002450:Cell Communication / Q000235:genetics / Q000276:immunology*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D005786:Gene Expression Regulation / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis / Q000502:physiology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D017455:Receptors, IgE / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism
chemical_list: D053645:Interleukin-2 Receptor alpha Subunit; D017455:Receptors, IgE