In vitro translation using HeLa extract.
A HeLa cell extract can be used as an alternative to rabbit reticulocyte lysate (RRL) for in vitro translation of mRNAs. It is particularly suited for synthesis of proteins that cannot be translated by RRL, is more cap dependent, has a higher capacity for faithful initiation at the correct start codon, and is more likely representative of translation in intact mammalian cells. This unit provides a detailed protocol for production and use of an mRNA-dependent cell-free translation system from HeLa extract. A support protocol describes production and purification of the mRNA.
Witherell
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RiboGene Inc., Hayward, California.
D002457:Cell Extracts; D002474:Cell-Free System; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008967:Molecular Biology / Q000379:methods*; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification
D002457:Cell Extracts; D012333:RNA, Messenger