Metabolic labeling with sulfate.
Post-translational modifications of proteins make it possible to determine where a protein normally resides or to follow its transport through the cell. One such modification is addition of sulfate either to tyrosine residues or to carbohydrate side chains. Labeling studies with [(35)S] sulfate can be done as continuous or pulse-chase experiments, as described here.
Tooze
S A
SA
Imperial Cancer Research Fund, London, United Kingdom.
D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D013462:Sulfur Radioisotopes / Q000032:analysis*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D007202:Indicators and Reagents; D011506:Proteins; D013462:Sulfur Radioisotopes; C005662:tyrosine O-sulfate; D014443:Tyrosine