Sex identification of owls (family Strigidae) using oligonucleotide microarrays.
Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species.
Wang
Lih Chiann
LC
Graduate Institute of Veterinary Medicine, National Taiwan University, 1 Sec. 4, Roosevelt Road, Taipei 106, Taiwan.
Severinghaus
Lucia Liu
LL
Chen
Chi Tsong
CT
Liu
Lu Yuan
LY
Pan
Chu Hsiang
CH
Huang
Dean
D
Lee
Hsiao Yuan
HY
Lir
Jihn Tsair
JT
Chin
Shih Chien
SC
Pu
Chang En
CE
Wang
Ching Ho
CH
D000818:Animals; D005260:Female; D006579:Heterozygote; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020305:Strigiformes / Q000235:genetics*