title: A strategy for the covalent functionalization of resorbable polymers with heparin and osteoinductive growth factor.
abstract: The chemical strategy presented herein is the nondestructive preparation of resorbable polymer scaffolds with heparin covalently bonded to the surface and an osteoinductive growth factor, recombinant human bone morphogenetic protein-2, immobilized in the heparin layer. The coupling scheme involves functionalization of surfaces by grafting in the vapor phase with poly( l-lactide) and poly(-caprolactone) films chosen as representative substrates. The biocompatibility of functionalized surfaces was verified by a much improved attachment and proliferation of mesenchymal stem cells (MSC).
authors.affiliation: Fibre and Polymer Technology, Royal Institute of Technology, SE-100 44 Stockholm, Sweden.
authors.forename: Ulrica
authors.initials: U
authors.lastname: Edlund
authors.forename: Staffan
authors.initials: S
authors.lastname: Dånmark
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Albertsson
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry* / Q000494:pharmacology; D018786:Bone Substitutes; D002460:Cell Line; D049109:Cell Proliferation; D006493:Heparin / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D051379:Mice; D011091:Polyesters / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D011091:Polyesters; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C016240:polycaprolactone; C033616:poly(lactide); D006493:Heparin