title: Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.
abstract: A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.
authors.affiliation: Department of Land, Air, and Water Resources, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA. minoue@ucdavis.edu
authors.forename: Mamie
authors.initials: M
authors.lastname: Nozawa-Inoue
authors.forename: Mercy
authors.initials: M
authors.lastname: Jien
authors.forename: Nicholas S
authors.initials: NS
authors.lastname: Hamilton
authors.forename: Valley
authors.initials: V
authors.lastname: Stewart
authors.forename: Kate M
authors.initials: KM
authors.lastname: Scow
authors.forename: Krassimira R
authors.initials: KR
authors.lastname: Hristova
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010472:Perchlorates / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010472:Perchlorates; D010088:Oxidoreductases; C494474:perchlorate