title: [Clinical value of human cytomegalovirus phosphoprotein 65 in the diagnosis of cytomegalovirus disease].
abstract: OBJECTIVE
  To establish a convenient method for diagnosis of active human cytomegalovirus (HCMV) infection in renal transplant recipients.
  
  
  METHODS
  The expression of HMCV phosphoprotein 65 (HCMV pp65) antigen in the peripheral blood leucocytes was detected by catalyzed signal amplification (CSA), and the results were compared with those by detection of the serum HCMV pp65 using anti-HCMV-IgM.
  
  
  RESULTS
  Of the 100 renal transplant recipients, 47 were positive for HCMV pp65 in the peripheral blood leucocytes, with the antigen-positive cell number of (71-/+45)/2x10(5) WBC, and 41 recipients were positive for anti-HCMV-IgM. In the 29 recipients presenting symptoms of HCMV infection, the antigen-positive cell number averaged (83-/+46)/2x 10(5) WBC. All the 50 healthy control subjects showed negative results of pp65 and anti-HCMV-IgM. The diagnostic sensitivity, specificity, positive predictive value and negative predictive value of this leukocyte-based HCMV pp65 detection were 100%, 74.6%, 61.7% and 100%, respectively.
  
  
  CONCLUSIONS
  This method provides a sensitive and convenient approach for diagnosis of HCMV infection, which may also provide evidence for adequate administration of the antiviral treatment.
authors.affiliation: Department of Clinical Laboratory, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. angieyangyang@sina.com.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010750:Phosphoproteins / Q000032:analysis*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014184:Transplantation, Homologous; D014763:Viral Matrix Proteins / Q000032:analysis*
chemical_list: D000956:Antigens, Viral; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa