title: A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis.
abstract: The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.
authors.affiliation: Center for Microbial Genetics and Genomics, Northern Arizona University, Flagstaff, AZ 86011-5640, United States.
authors.forename: L J
authors.initials: LJ
authors.lastname: Kenefic
authors.forename: J
authors.initials: J
authors.lastname: Beaudry
authors.forename: C
authors.initials: C
authors.lastname: Trim
authors.forename: L
authors.initials: L
authors.lastname: Huynh
authors.forename: S
authors.initials: S
authors.lastname: Zanecki
authors.forename: M
authors.initials: M
authors.lastname: Matthews
authors.forename: J
authors.initials: J
authors.lastname: Schupp
authors.forename: M
authors.initials: M
authors.lastname: Van Ert
authors.forename: P
authors.initials: P
authors.lastname: Keim
mesh_terms: D000483:Alleles; D001408:Bacillus anthracis / Q000145:classification* / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D019075:Electrophoresis, Capillary; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004269:DNA, Bacterial