[Study on the application of pulsed-field gel electrophoresis regarding infection sources identification during an outbreak of Vibrio cholerae in Jiangxi Province].
OBJECTIVE
To study the correlation between Vibrio cholerae strains isolated from natural enviroment and fishery products and the source of infection during V. cholerae outbreaks.


METHODS
Cholera toxin gene was detected by polymerase chain reaction (PCR) amplification. Pulsed-field gel electrophoresis (PFGE) was used to subtype the isolates. Results of PFGE were analyzed and clustered by BioNumerics software (Version 4.0).


RESULTS
During the outbreaks, a total number of thirty O139 V. cholerae related serogroups were collected from patients, carriers, sewage and fishery products were identified and proved to be toxigenic. They could be clustered into four PFGE patterns when digested by Not I. These two V. cholerae outbreaks were caused by the same source of infection because of the following reasons: (1) PFGE patterns of the predominant strains isolated from two outbreaks were identical; (2) they were identical to the PFGE patterns of the strains isolated from the green turtle and rana catesbiana which were bought from the same wholesale store.


CONCLUSIONS
Green turtle and rana catesbiana that were contaminated by toxigenic O139 V. cholerae strains seemed to be the source of infection causing the O139 V. cholerae outbreaks in Jiangxi province. Rapid laboratory surveillance and epidemiologic investigation were important in identifying the source of infection during the outbreaks of V. cholera.
Yang
Meng
M
Jiangxi Center for Disease Control and Provintion, Nanchang 330029, China.
Diao
Bao-Wei
BW
Cheng
Hui-Jian
HJ
Ding
Sheng
S
Cui
Zhi-Gang
ZG
Chen
Fu-Hui
FH
Xu
Xiao-Qian
XQ
Kan
Biao
B
Yuan
Hui
H
D000818:Animals; D002681:China / Q000453:epidemiology; D002771:Cholera / Q000453:epidemiology*; D016000:Cluster Analysis; D004196:Disease Outbreaks; D004197:Disease Reservoirs / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005398:Fisheries; D006801:Humans; D011898:Ranidae / Q000382:microbiology; D012722:Sewage / Q000382:microbiology; D014426:Turtles / Q000382:microbiology; D041242:Vibrio cholerae O139 / Q000302:isolation & purification*
D012722:Sewage