Interleukin-4 (C-590T) and interferon-gamma (G5644A) gene polymorphisms in patients with periodontitis.
OBJECTIVE
Periodontitis is a multifactorial disease and immunologic and genetic factors have an important role in its pathogenesis. Mutation in the promoter regions of the interleukin-4 and interferon-gamma genes has been reported to modify the protein expression. The objective of this study was to evaluate the possible role of interleukin-4 (C-590T) and interferon-gamma (G5644A) polymorphisms in the susceptibility to periodontitis.


METHODS
In this case-control study, 53 patients (36 women and 17 men), comprising 27 patients with aggressive periodontitis and 26 patients with chronic periodontitis, and 56 healthy volunteers, were enrolled. DNA was isolated from all subjects, and the polymerase chain reaction-sequence specific primer method was used to study the interleukin-4 (C-590T) and interferon-gamma (G5644A) gene polymorphisms.


RESULTS
Our results showed no significant difference in the allele and genotype frequencies of interleukin-4 (C-590T) and interferon-gamma (G5644A) gene polymorphisms between patients with periodontal disease and controls.


CONCLUSIONS
The results suggest that the interleukin-4 (C-590T) and interferon-gamma (G5644A) gene polymorphisms may not be associated with the susceptibility of Iranian individuals to periodontitis.
Hooshmand
B
B
Department of Peridontology, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
Hajilooi
M
M
Rafiei
A
A
Mani-Kashani
K H
KH
Ghasemi
R
R
D000293:Adolescent; D000328:Adult; D000483:Alleles; D002908:Chronic Disease; D004812:Epidemiologic Methods; D005260:Female; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000235:genetics*; D015847:Interleukin-4 / Q000097:blood / Q000235:genetics*; D007492:Iran; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*
D015847:Interleukin-4; D007371:Interferon-gamma