Mucin degradation by Bifidobacterium strains isolated from the human intestinal microbiota.
The presence of the genes engBF (endo-alpha-N-acetylgalactosaminidase) and afcA (1,2-alpha-L-fucosidase) was detected in several intestinal Bifidobacterium isolates. Two strains of Bifidobacterium bifidum contained both genes, and they were able to degrade high-molecular weight porcine mucin in vitro. The expression of both genes was highly induced in the presence of mucin.
Ruas-Madiedo
Patricia
P
Departamento de Microbiología y Bioquímica de Productos Lácteos, Instituto de Productos Lácteos de Asturias, CSIC, Carretera de Infiesto s/n, 33300 Villaviciosa, Asturias, Spain.
Gueimonde
Miguel
M
Fernández-García
María
M
de los Reyes-Gavilán
Clara G
CG
Margolles
Abelardo
A
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008969:Molecular Sequence Data; D009077:Mucins / Q000378:metabolism*; D010802:Phylogeny; D013552:Swine; D005644:alpha-L-Fucosidase / Q000235:genetics / Q000378:metabolism; D048809:alpha-N-Acetylgalactosaminidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D009077:Mucins; D048809:alpha-N-Acetylgalactosaminidase; D005644:alpha-L-Fucosidase