title: In vitro analysis of endoplasmic-reticulum-to-golgi transport in mammalian cells.
abstract: A temperature-sensitive mutant of vesicular stomatitis G protein is used to follow the movement of that protein from the endoplasmic reticulum to transport vesicles to cis-Golgi and finally medial/trans-Golgi by assessing the maturation of two asparagine-linked oligosaccharides. These assays can be used to identify the factors that are required for and regulate protein trafficking through these compartments.
authors.affiliation: The Scripps Research Institute, La Jolla, California, USA.
authors.forename: B B
authors.initials: BB
authors.lastname: Allan
authors.forename: W E
authors.initials: WE
authors.lastname: Balch
mesh_terms: D020823:ADP-Ribosylation Factor 1; D000818:Animals; D001216:Asparagine / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000821:virology; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism* / Q000821:virology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D033921:Vesicular Transport Proteins; D018116:Vesiculovirus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D033921:Vesicular Transport Proteins; D014759:Viral Envelope Proteins; D001216:Asparagine; D020823:ADP-Ribosylation Factor 1