Regulation of interferon signaling by the C and V proteins from attenuated and wild-type strains of measles virus.
The C and V proteins of the measles virus (MV) have been shown to block the signaling of type I and II interferon (IFN-alpha/beta and IFN-gamma). The relative contribution of the C and V proteins to the inhibition of IFN signaling and the extent to which this activity differs in attenuated or wild-type strains of MV remains undefined. This study presents a comparison of the IFN-antagonist activities of C and V proteins from four attenuated and two wild-type strains of MV. The V proteins were more potent inhibitors of IFN-inducible reporter gene expression than the C proteins, and this effect was unrelated to whether the protein originated from an attenuated or wild-type strain. The results also demonstrated the importance of the tyrosine at position 110 in the inhibition of IFN-alpha/beta and IFN-gamma signaling by the V protein, and identified a non-recombinant MV expressing a V protein that was impaired due to a mutation at this residue.
Fontana
Judith M
JM
Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Centers for Disease Control and Prevention, Mailstop C-22, 1600 Clifton Road, Atlanta, Georgia 30333, USA.
Bankamp
Bettina
B
Bellini
William J
WJ
Rota
Paul A
PA
D019943:Amino Acid Substitution; D019677:Artificial Gene Fusion; D017930:Genes, Reporter; D007372:Interferons / Q000037:antagonists & inhibitors*; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010750:Phosphoproteins / Q000276:immunology; D017422:Sequence Analysis, DNA; D014443:Tyrosine / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D010750:Phosphoproteins; C069181:V protein, measles virus; D014764:Viral Proteins; D014443:Tyrosine; D007372:Interferons; D008156:Luciferases