title: [Construction of a genetic map based on ILP markers in rice].
abstract: Intron length polymorphism (ILP) is a new type of PCR-based molecular markers with many advantages. We had previously developed 172 ILP markers in rice using the published genome sequence data of indica cultivar 93-11 and ja-ponica cultivar Nipponbare. In order to examine the reliability and the applicability of these ILP markers to genetic map-ping, we constructed a rice genetic map consisting of 172 ILP and 13 SSR markers with a total length of 1 905.7 cM using a BC1F1 (Nipponbare/93-11//Nipponbare) population. Comparison showed that the order of the markers in this map was ex-actly the same as their physical order, verifying the feasibility and efficiency of using ILP markers for genetic mapping. We also investigated the phenomenon of marker segregation distortion and found a region of serious segregation distortion in the short arm of chromosome 6.
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China. forwardzhao@yahoo.com
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Wei-Ren
authors.initials: WR
authors.lastname: Wu
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics; D005819:Genetic Markers; D007438:Introns / Q000235:genetics*; D012275:Oryza / Q000166:cytology / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers