Fabrication, characterization, and application of potentiometric immunosensor based on biocompatible and controllable three-dimensional porous chitosan membranes.
A novel three-dimensional porous chitosan membrane material was prepared as a matrix to encapsulate hepatitis B surface antibody (HBsAb) for fabrication of immunosensors. The porous chitosan matrix was prepared by electrodepositing a designer nanocomposite solution of chitosan-encapsulated silica nanoparticle hybrid film on an ITO electrode, and then removing the silica nanoparticles with HF solution. Using HBsAb as a model, the potentiometric immunosensor was constructed by linking HBsAb molecules to the three-dimensional porous chitosan film using glutaraldehyde as a cross-linker. Scanning electron microscopy was used to investigate the surface morphology of the three-dimensional porous chitosan films. Cyclic voltammograms and electrochemical impedance spectroscopy were used to probe the interfacial properties of the immunosensor. Results showed that the fabricated immunosensor with three-dimensional porous structure possessed high surface area, good mechanical stability, and good hydrophilicity, which provided a biocompatible microenvironment for maintaining the bioactivity of the immobilized protein and increased the protein loading. Therefore, the present immunosensor exhibits a wide linear range from 6.85 to 708 ng mL(-1) with a low detection limit of 3.89 ng mL(-1) for the detection of hepatitis B surface antigen (HBsAg). This work implied that the biocompatible and controllable three-dimensional porous chitosan membrane possessed potential applications for biosensing.
Liang
Ruping
R
Department of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang 330031, People's Republic of China. rpliang@ncu.edu.cn
Peng
Hongzhen
H
Qiu
Jianding
J
D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan; D004355:Drug Stability; D004563:Electrochemistry; D006510:Hepatitis B Antibodies / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D008567:Membranes, Artificial; D053758:Nanoparticles; D011199:Potentiometry / Q000379:methods*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D008567:Membranes, Artificial; D048271:Chitosan