Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine.
Periodontitis is a periodontal tissue infectious disease and the most common cause for tooth loss in adults. It has been linked to many systemic disorders, such as coronary artery disease, stroke, and diabetes. At present, there is no ideal therapeutic approach to cure periodontitis and achieve optimal periodontal tissue regeneration. In this study, we explored the potential of using autologous periodontal ligament stem cells (PDLSCs) to treat periodontal defects in a porcine model of periodontitis. The periodontal lesion was generated in the first molars area of miniature pigs by the surgical removal of bone and subsequent silk ligament suture around the cervical portion of the tooth. Autologous PDLSCs were obtained from extracted teeth of the miniature pigs and then expanded ex vivo to enrich PDLSC numbers. When transplanted into the surgically created periodontal defect areas, PDLSCs were capable of regenerating periodontal tissues, leading to a favorable treatment for periodontitis. This study demonstrates the feasibility of using stem cell-mediated tissue engineering to treat periodontal diseases.
Liu
Yi
Y
Salivary Gland Disease Center and the Molecular Laboratory for Gene Therapy, Capital Medical University School of Stomatology, Beijing 100050, China.
Zheng
Ying
Y
Ding
Gang
G
Fang
Dianji
D
Zhang
Chunmei
C
Bartold
Peter Mark
PM
Gronthos
Stan
S
Shi
Songtao
S
Wang
Songlin
S
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D010513:Periodontal Ligament / Q000166:cytology / Q000637:transplantation*; D010518:Periodontitis / Q000473:pathology / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology; D013552:Swine; D013556:Swine, Miniature; D016896:Treatment Outcome