Experimental study on the relation between the noninvasive ultrasonography quantitative scoring system and the degree of the hepatic fibrosis.
OBJECTIVE
The fibrosis models were constructed in the experimental rats which underwent complex fibrosis models methods, and the aim was to assess the value of noninvasive ultrasonography quantitative scoring system in the diagnosis of hepatic fibrosis using high-frequency ultrasonography unit.


METHODS
Thirty-five male rats were included in this study. The fibrosis models were constructed in the 35 experimental rats which underwent complex fibrosis models methods. Ultrasonography, serum fibrosis markers were submitted in week 0, week 2, week 4, and week 6, during the construction of fibrosis models, which were also detected with pathological diagnosis. Ultrasonography quantitative scoring system was used to discriminate the degree of hepatic fibrosis, and the results were compared with pathological diagnosis.


RESULTS
The complex fibrosis models methods can be used to construct hepatic fibrosis model. The ultrasonography quantitative scoring system had a higher relativity to pathological stage (r=0.86), when it was combined with serum fibrosis markers, the sensitivity (96.5%) and specificity (95.8%) were increased.


CONCLUSIONS
High-frequency ultrasonography appears to be an efficient tool allowing new possibilities to use this animal model, ultrasonography quantitative scoring system, especially when it was combined with CT, serum fibrosis markers, had a higher value in noninvasive quantitative diagnosis of hepatic fibrosis in longitudinal studies, which are much more powerful.
Yan
Guozhen
G
Department of Ultrasound Diagnostics, Tangdu Hospital, The Fourth Military Medical University Xi'an, China. ygz670708@163.com
Duan
Yunyou
Y
Ruan
Litao
L
Yang
YiLin
Y
Cao
TieSheng
T
D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000145:classification / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012372:ROC Curve; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014463:Ultrasonography
D018809:Proliferating Cell Nuclear Antigen