Detection and quantitation of radiolabeled proteins in gels and blots.
Permanent records of the results of electrophoretic separations of radiolabeled proteins and membrane-bound proteins (and RNA and DNA) can be made using autoradiography, fluorography, and phosphor imaging. These images can subsequently be quantified using densitometry to obtain a relative measure of the amount of radioactivity in a sample. This unit also includes descriptions of image-enhancement techniques and guidelines for selection of appropriate recording media.
Voytas
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D
Iowa State University, Ames, Iowa, USA.
Ke
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N
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001965:Bromides / Q000528:radiation effects; D003720:Densitometry / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005470:Fluorometry / Q000379:methods; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D011856:Radiographic Image Enhancement / Q000295:instrumentation / Q000379:methods; D011868:Radioisotopes / Q000032:analysis*; D011874:Radiometry / Q000379:methods; D018030:Silver Compounds / Q000528:radiation effects
D001965:Bromides; D011506:Proteins; D011868:Radioisotopes; D018030:Silver Compounds; C047751:silver bromide