Bioproduction of ascorbic acid in root nodule and root of the legume pulse Phaseolus mungo.
The root nodules of Phaseolus mungo (L.), a herbaceous leguminous pulse, contain high amounts of ascorbic acid (AsA). A glucose pool present in the nodule might serve as precursor for AsA production. From root nodule, a Rhizobium sp. was isolated. The symbiont produced a large amount of AsA (290.5 microg/ml) from glucose-supplemented basal medium. The production of AsA by the symbiont was much greater than that of the control when the glucose (0.5%)-supplemented mineral medium was enriched with thiamine hydrochloride (20 microg/100 ml), biotin (20 microg/100 ml), and L-asparagine (0.2%). The possible role of the rhizobial production of AsA on rhizobia-legume symbiosis is discussed.
Ghosh
S
S
Department of Botany, Burdwan University, Golapbag, Burdwan, 713104 West Bengal, India.
Maiti
T K
TK
Basu
P S
PS
D001205:Ascorbic Acid / Q000378:metabolism*; D027805:Phaseolus / Q000378:metabolism / Q000382:microbiology; D012231:Rhizobium / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000378:metabolism / Q000382:microbiology; D013559:Symbiosis
D001205:Ascorbic Acid