[The genetic effects of IGF2 gene intron3 variance in pigs].
A mispairing PCR-RFLP technique was applied in this study to determine the Insulin-like Growth Factor 2(IGF2) gene intron3 G3072A mutation in an outbred Landrace and Large White, and the gelded boars from Landrace x Large White cross. The difference of corresponding traits and the genetic effects of the boars inherited from parental A allele and inherited from parental G allele were analyzed. The results indicated that comparing with the boars inherited from parental G allele, the boars inherited from parental A allele increased significantly in the circumference 3.06% (P< 0.05) and index of body 3.01% (P< 0.05), respectively. The boars inherited from parental A allele had a significantly less average buttock fat thickness (15.31%, P< 0.01), thorax-waist fat thickness (23.74%, P< 0.01), skin thickness 9.38% (P< 0.01), fiber density (20.03%, P< 0.01) and had more less 6th-7th rib fat thickness (20.27%, P< 0.05), tendernce (17.32%, P< 0.05), and had more thick shoulder fat thickness (7.97%, P< 0.05), and had bigger the loin eye area (22.58%, P< 0.01) and fiber cross-sectional area (32.70%, P< 0.01) and fiber diameter (15.38%, P< 0.01) and lean meat (2.18%, P< 0.01) than the boars inherited from parental G allele. The results were suggested that the parental A allele has highly significant genetic effects in improving pig body development and carcass lean percent by increasing fiber diameter and the loin eye area, and decreasing the skin thickness and fat percent.
Yu
De-Bing
DB
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. yudb79@163.com
He
Zong-Liang
ZL
Zhang
Wei-Feng
WF
Jia
Xiao-Xu
XX
Qiu
Xin-Shen
XS
Wang
Lin-Yun
LY
Huang
Rui-Hua
RH
Liu
Hong-Lin
HL
D000483:Alleles; D000818:Animals; D049628:Body Size / Q000235:genetics; D005260:Female; D005838:Genotype; D007438:Introns / Q000235:genetics*; D008297:Male; D008460:Meat; D009132:Muscles / Q000378:metabolism; D009154:Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013002:Somatomedins / Q000235:genetics*; D013552:Swine / Q000033:anatomy & histology* / Q000235:genetics* / Q000502:physiology
D013002:Somatomedins