[The use of fluorescent liposomal immunoanalysis for the diagnostics and indication of glanders and melioidosis].
The authors have developed a homogenous method for the detection of the cells, surface antigen, and Ag8 of glanders and melioidosis pathogens and antibodies to them, based on the compliment-dependent lysis of Ag8-sensitized liposomes. The method is highly specific; its sensitivity to antibodies to glanders and melioidosis pathogens is 24 to 440 times higher than that of RID: its sensitivity to Ag8 is 100 ng/ml corresponding to 1.25x10-10 M, and its sensitivity to cells is 10(5) to 10(6) cells/ml).
Skopinskaia
S N
SN
Iarkov
S P
SP
Khramov
E N
EN
Khrapova
N P
NP
Piven'
N N
NN
Prokhvatilova
E V
EV
D000942:Antigens, Bacterial / Q000032:analysis*; D042726:Burkholderia mallei / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D015200:Fluoroimmunoassay / Q000379:methods*; D005896:Glanders / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis* / Q000382:microbiology; D015203:Reproducibility of Results
D000942:Antigens, Bacterial