title: Evaluation of polymerase chain reaction (PCR) method and hybrid capture II (HCII) assay for the detection of human papillomavirus in cervical scrapings.
abstract: In order to investigate the reliability of detecting HPV DNA in cervical smears, we compared the performance of nested MY/GP PCR and FDA approved-Hybrid Capture II (HCII) using clinical cervical scrapings from 40 patients. It was found that PCR was more sensitive (81.8%) in comparison to HCII (36.4%) in detecting HPV although specificity of HCII was much higher (96.6%) than PCR (58.6%). The Negative Predictive Value (NPV) of both the techniques were quite similar but Positive Predictive Value (PPV) of HCII was much higher (80.0%) compared to PCR (42.9%). While the HCII method showed good specificity for HPV detection, its lack of sensitivity as compared to PCR may be a drawback for diagnostic use.
authors.affiliation: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan.
authors.forename: R
authors.initials: R
authors.lastname: Saini
authors.forename: T H
authors.initials: TH
authors.lastname: Shen
authors.forename: N H
authors.initials: NH
authors.lastname: Othman
authors.forename: J
authors.initials: J
authors.lastname: Santhanam
authors.forename: N
authors.initials: N
authors.lastname: Othman
authors.forename: T H
authors.initials: TH
authors.lastname: Tang
mesh_terms: D003581:Cytodiagnosis; D005260:Female; D006801:Humans; D008296:Malaysia; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000821:virology; D014626:Vaginal Smears