Two-photon excitation of fluorescence from chicken tissue.
We have measured UV fluorescence excited through two-photon absorption from native chicken tissue, using 600-nm, 500-fs pulses from a R6G dye laser. The observed emission signal was found to depend quadratically on the excitation intensity. The two-photon excitation-induced fluorescence spectrum is attributed to tryptophan residues in proteins.
Guo
Y
Y
Department of Electrical Engineering, Institute for Ultrafast Spectroscopy and Lasers and New York State Center for Advanced Technology for Ultrafast Photonic Materials and Applications, The City College and Graduate School of The City University of New York, New York, New York 10031, USA.
Wang
Q Z
QZ
Zhadin
N
N
Liu
F
F
Demos
S
S
Calistru
D
D
Tirksliunas
A
A
Katz
A
A
Budansky
Y
Y
Ho
P P
PP
Alfano
R R
RR