Application of atomic force microscopy in morphological observation of antisense probe labeled with magnetism.
OBJECTIVE
To explore the possibility of the c-erbB2 oncogene antisense probe labeled with superparamagnetic iron oxide (SPIO) nanoparticles as a target contrast agent for magnetic resonance (MR) imaging whose morphology was observed with atomic force microscopy (AFM), and its efficiency was examined by MR imaging.


METHODS
The c-erbB2 oncogene antisense probe labeled with SPIO was synthesized by a chemical cross-linking approach. Its morphology was observed with AFM.


RESULTS
The chemical constitution of c-erbB2 oncogene antisense probes can be observed with AFM. The molecular structure of probes is easily visualized under AFM. Probes with diameters of 25-40 nm are in order, follow uniformity and the arrangement rule, can be separated from each other, and appear as cubes with a rugged surface morphology. Strong, low signals of the probes in transfected cells were observed by MR cellular imaging.


CONCLUSIONS
AFM is ideal for morphological observation and for analyzing the molecular structure of synthesized c-erbB2 oncogene antisense probes.
Wen
Ming
M
Department of Radiology, The First Affiliated Hospital, College, Chongqing Medical University, Chongqing, China.
Li
Bibo
B
Bai
Wei
W
Li
Shaolin
S
Yang
Xueheng
X
D016375:Antisense Elements (Genetics) / Q000235:genetics; D045744:Cell Line, Tumor; D003287:Contrast Media; D018734:Genes, erbB-2; D006801:Humans; D058085:Iron Compounds; D008279:Magnetic Resonance Imaging; D008280:Magnetics; D018625:Microscopy, Atomic Force; D053758:Nanoparticles; D010087:Oxides; D024862:Oxyphil Cells / Q000648:ultrastructure*; D014162:Transfection
D016375:Antisense Elements (Genetics); D003287:Contrast Media; D058085:Iron Compounds; D010087:Oxides