Imaging tumor cell movement in vivo.
This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used to provide a mammalian system for studying human tumor cells as xenografts in immunocompromised mice, as well as for following the development of tumors from a specific tissue type in transgenic lines. The Basic Protocol describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included.
Kedrin
Dmitriy
D
Albert Einstein College of Medicine, Bronx, New York, USA.
Wyckoff
Jeffrey
J
Sahai
Erik
E
Condeelis
John
J
Segall
Jeffrey E
JE
D000230:Adenocarcinoma / Q000098:blood supply / Q000556:secondary*; D000818:Animals; D002408:Catheters, Indwelling; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D008264:Macrophages / Q000166:cytology; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection; D014966:Xanthenes / Q000032:analysis
C485182:Cyan Fluorescent Protein; D014966:Xanthenes; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C034657:Texas red