The histone deacetylase inhibitor butyrate inhibits melanoma cell invasion of Matrigel.
BACKGROUND
Histone deacetylase (HDAC) inhibitors have anticancer effects. Their effects on expression of cell adhesion molecules might be related to their effects on tumor cell invasion.


METHODS
Murine B16-BL6 cells were treated with the HDAC inhibitors, butyrate or trichostatin A. Melanoma cell invasion of the artificial basement membrane, Matrigel, was examined by Transwell chamber assay.


RESULTS
Butyrate as well as trichostatin A inhibited the cell growth mainly by arresting the cell cycle. The cell invasion of Matrigel was inhibited by butyrate and trichostatin A. The butyrate treatment increased the cell-cell aggregation, although neither E-cadherin nor N-cadherin mRNA were up-regulated. Both mRNA expression and protein levels of the immunoglobulin superfamily cell adhesion molecules, Mel-CAM and L1-CAM, were increased in the butyrate-treated cells.


CONCLUSIONS
The HDAC inhibitor butyrate blocked the B16-BL6 melanoma cell invasion of Matrigel, although it increased the expression of Mel-CAM and L1-CAM which are important to the metastatic potential.
Kuwajima
Akiko
A
Molecular Biology Laboratory, Akita Prefectural University, Akita 010-0195, Japan.
Iwashita
Jun
J
Murata
Jun
J
Abe
Tatsuya
T
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D003094:Collagen; D004338:Drug Combinations; D004791:Enzyme Inhibitors / Q000494:pharmacology; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids / Q000494:pharmacology; D007797:Laminin; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D051379:Mice; D009361:Neoplasm Invasiveness; D011509:Proteoglycans
D002087:Butyrates; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; C012589:trichostatin A; D003094:Collagen