Non-radioactive quantification of fibronectin matrix assembly.
This unit provides a basic protocol for non-radioactive determination of the rate of incorporation of biotinylated fibronectin into the insoluble matrix organized by cultured cells. The protocol provides quantification of changes in matrix assembly due to different experimental conditions with concomitant determinations of the activation state of various signaling molecules that may be involved in the process of matrix assembly. This unit also provides a support protocol for biotinylation of purified fibronectin.
Pankov
Roumen
R
National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.
Yamada
Kenneth M
KM
D000818:Animals; D019921:Biotinylation / Q000379:methods; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis* / Q000378:metabolism*; D006801:Humans
D005353:Fibronectins