title: Dialysis and concentration of protein solutions.
abstract: Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. Dialysis is usually used to change the salt (small-molecule) composition of a macromolecule-containing solution. This unit describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A support protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.
authors.affiliation: Chester College, Chester, United Kingdom.
authors.forename: S M
authors.initials: SM
authors.lastname: Andrew
authors.forename: J A
authors.initials: JA
authors.lastname: Titus
authors.forename: L
authors.initials: L
authors.lastname: Zumstein
mesh_terms: D002482:Cellulose; D003956:Dialysis / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D015314:Dialysis Solutions; D004058:Diffusion; D008567:Membranes, Artificial; D008970:Molecular Weight; D010455:Peptides; D011506:Proteins; D018401:Sample Size; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods
chemical_list: D015314:Dialysis Solutions; D008567:Membranes, Artificial; D010455:Peptides; D011506:Proteins; D002482:Cellulose