title: Origin and evolution of candidate mental retardation genes on the human X chromosome (MRX).
abstract: BACKGROUND
  The human X chromosome has a biased gene content. One group of genes that is over-represented on the human X are those expressed in the brain, explaining the large number of sex-linked mental retardation (MRX) syndromes.
  
  
  RESULTS
  To determine if MRX genes were recruited to the X, or whether their brain-specific functions were acquired after relocation to the mammalian X chromosome, we examined the location and expression of their orthologues in marsupials, which diverged from human approximately 180 million years ago. We isolated and mapped nine tammar wallaby MRX homologues, finding that six were located on the tammar wallaby X (which represents the ancient conserved mammal X) and three on chromosome 5, representing the recently added region of the human X chromosome. The location of MRX genes within the same synteny groups in human and wallaby does not support the hypothesis that genes with an important function in the brain were recruited in multiple independent events from autosomes to the mammalian X chromosome. Most of the tammar wallaby MRX homologues were more widely expressed in tammar wallaby than in human. Only one, the tammar wallaby ARX homologue (located on tammar chromosome 5p), has a restricted expression pattern comparable to its pattern in human. The retention of the brain-specific expression of ARX over 180 million years suggests that this gene plays a fundamental role in mammalian brain development and function.
  
  
  CONCLUSIONS
  Our results suggest all the genes in this study may have originally had more general functions that became more specialised and important in brain function during evolution of humans and other placental mammals.
authors.affiliation: Comparative Genomics Group, Research School of Biological Sciences, The Australian National University, Canberra, ACT 2601, Australia. Margaret.Delbridge@anu.edu.au
authors.forename: Margaret L
authors.initials: ML
authors.lastname: Delbridge
authors.forename: Daniel A
authors.initials: DA
authors.lastname: McMillan
authors.forename: Ruth J
authors.initials: RJ
authors.lastname: Doherty
authors.forename: Janine E
authors.initials: JE
authors.lastname: Deakin
authors.forename: Jennifer A Marshall
authors.initials: JA
authors.lastname: Graves
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D041321:Chromosomes, Human, X / Q000235:genetics*; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D050172:Genes, X-Linked / Q000235:genetics*; D006801:Humans; D007614:Macropodidae / Q000235:genetics; D038901:X-Linked Intellectual Disability / Q000235:genetics*; D051379:Mice; D012689:Sequence Homology, Nucleic Acid