title: Molecular homogeneity in diverse geographical populations of Phlebotomus papatasi (Diptera, Psychodidae) inferred from ND4 mtDNA and ITS2 rDNA Epidemiological consequences.
abstract: An intraspecific study on Phlebotomus papatasi, the main proven vector of Leishmania major among the members of the subgenus Phlebotomus, was performed. The internal transcribed spacer 2 (ITS 2) of rDNA and the ND4 gene of mt DNA were sequenced from 26 populations from 18 countries (Albania, Algeria, Cyprus, Egypt, Greece, India, Iran, Israel, Italy, Lebanon, Morocco, Saudi Arabia, Spain, Syria, Tunisia, Turkey, Yugoslavia and Yemen), and compared. Samples also included three other species belonging to the subgenus Phlebotomus: P. duboscqi, a proven vector of L. major in the south of Sahara (three populations from Burkina Faso, Kenya and Senegal), P. bergeroti, a suspected vector of L. major (three populations from Oman Sultanate, Iran and Egypt), and one population of P. salehi from Iran. A phylogenetic study was carried out on the subgenus Phlebotomus. Our results confirm the validity of the morphologically characterized taxa. The position of P. salehi is doubtful. Variability in P. papatasi contrasts with that observed within other species having a wide distribution like P. (Paraphlebotomus) sergenti in the Old World or Lutzomyia (Lutzomyia) longipalpis in the New World. Consequently, it could be hypothesized that all populations of P. papatasi over its distribution area have similar vectorial capacities. The limits of the distribution area of L. major are correlated with the distribution of common rodents acting as hosts of the parasites.
authors.affiliation: USC "VECPAR", AFSSA, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, Reims, France. jerome.depaquit@univ-reims.fr
authors.forename: Jérôme
authors.initials: J
authors.lastname: Depaquit
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Lienard
authors.forename: Astrid
authors.initials: A
authors.lastname: Verzeaux-Griffon
authors.forename: Hubert
authors.initials: H
authors.lastname: Ferté
authors.forename: Azzedine
authors.initials: A
authors.lastname: Bounamous
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Gantier
authors.forename: Hanafi A
authors.initials: HA
authors.lastname: Hanafi
authors.forename: Raymond L
authors.initials: RL
authors.lastname: Jacobson
authors.forename: Michele
authors.initials: M
authors.lastname: Maroli
authors.forename: Vahideh
authors.initials: V
authors.lastname: Moin-Vaziri
authors.forename: Frédérique
authors.initials: F
authors.lastname: Müller
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Ozbel
authors.forename: Milena
authors.initials: M
authors.lastname: Svobodova
authors.forename: Petr
authors.initials: P
authors.lastname: Volf
authors.forename: Nicole
authors.initials: N
authors.lastname: Léger
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000032:analysis*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D006239:Haplotypes; D006801:Humans; D009245:NADH Dehydrogenase / Q000235:genetics*; D010691:Phlebotomus / Q000235:genetics*; D010217:Phlebotomus Fever / Q000453:epidemiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; C098483:NADH dehydrogenase subunit 4; D009245:NADH Dehydrogenase