[Experiment study of PHI on histone methylation and acetylation in Molt-4 cells].
OBJECTIVE
To investigate the effect of phenyl-hexyl isothiocyanate (PHI) on acetylation and methylation of histone in acute lymphoblastic leukemia cell line Molt4.


METHODS
The inhibition of cell proliferation was observed by MTT method and clone suppression test. Apoptosis and cell cycle arrest were measured by flow cytometry. The alterations in histone acetyltransferase and acetylation and methylation of histones were detected by Western blot.


RESULTS
PHI could up-regulate the expression of acetyltransferase (P300/CBP), markedly induced the accumulation of acetylated histone H3, H4 and methylated histone H3 lysine 4 (H3K4), and inhibited methylation on lysine 9 of H3 (H3K9). The epigenetic regulation resulted in cell cycle arrest at G0/G1 phase, and induction of apoptosis.


CONCLUSIONS
PHI can modulate both histone methylation and acetylation. It may serve as a histone deacetylase inhibitor, and might be a potential novel anti-leukemia agent.
Huang
Yi-Qun
YQ
Department of Hematology, Zhangzhou Municipal Hospital, Fujian Medical University, Zhangzhou 363000, China.
Ma
Xu-Dong
XD
Zhen
Rui-Ji
RJ
Chiao
Jen-Wei
JW
Liu
De-Long
DL
D000107:Acetylation / Q000187:drug effects; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D044127:Epigenesis, Genetic; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008745:Methylation / Q000187:drug effects
D006657:Histones; D017879:Isothiocyanates; C073279:6-phenylhexyl isothiocyanate; D006655:Histone Deacetylases