title: PAS-FTIR and FT-Raman qualitative characterization of sodium dodecyl sulfate interaction with an alternative stratum corneum model membrane.
abstract: The interaction of the surfactant sodium dodecyl sulfate with the stratum corneum (SC) of shed snake skin from Bothrops jararaca, used as a model membrane, was characterized qualitatively by FT-Raman and infrared photoacoustic (PAS-FTIR) spectroscopy, used as analytical tools. Surfactant solutions were 50.0 g x l(-1) and 2.34 g x l(-1) with treatment intervals of 4, 8 and 12 h. The employment of FT-Raman and PAS-FTIR indicated increased hydration of the SC with alteration of the tissue topography. The interaction of the SC with surfactant was increased by the tape-stripping process. The consequent exposure of the internal layers of the tissue intensified the effect of the anionic surfactant, indicating that this layer acted as an additional barrier.
authors.affiliation: Laboratory of Pharmaceutical Technology, Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, Brazil. andrerb@usp.br
authors.forename: A R
authors.initials: AR
authors.lastname: Baby
authors.forename: A C L
authors.initials: AC
authors.lastname: Lacerda
authors.forename: Y
authors.initials: Y
authors.lastname: Kawano
authors.forename: M V R
authors.initials: MV
authors.lastname: Velasco
authors.forename: T M
authors.initials: TM
authors.lastname: Kaneko
mesh_terms: D000818:Animals; D017837:Bothrops / Q000378:metabolism*; D066298:In Vitro Techniques; D007633:Keratins / Q000737:chemistry; D008566:Membranes / Q000737:chemistry; D012867:Skin / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013501:Surface-Active Agents / Q000737:chemistry*; D014732:Vibration
chemical_list: D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D007633:Keratins