title: Preparation of gelled substrates.
abstract: Gelled substrates can be used for a variety of in vitro and in vivo experiments. A type I collagen gelled substrate will promote cell growth and differentiation. Gelled Matrigel substrate promotes the survival of explanted cells and tissues and the differentiation of a variety of epithelial and endothelial cell types in vitro and to assess angiogenesis and increase tumor growth in vivo. Preparation of the matrices and their use are described in this unit.
authors.affiliation: National Institute of Dental Research/NIH, Bethesda, Maryland, USA.
authors.forename: H K
authors.initials: HK
authors.lastname: Kleinman
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000302:isolation & purification* / Q000378:metabolism*; D003094:Collagen / Q000302:isolation & purification* / Q000378:metabolism*; D024042:Collagen Type I / Q000302:isolation & purification / Q000378:metabolism; D003584:Cytological Techniques; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005782:Gels; D006801:Humans; D007797:Laminin / Q000302:isolation & purification* / Q000378:metabolism*; D011509:Proteoglycans / Q000302:isolation & purification* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D004338:Drug Combinations; D005782:Gels; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen