[Microbiological and clinical diagnosis of acute endophthalmitis].
The microbiological study identifies the bacterial spectrum after surgery, in acute, delayed-onset, or chronic endophthalmitis. DNA amplification of eubacterium-specific sequences in DNA extracted from ocular samples is a new tool for the etiological diagnosis of endophthalmitis. The most successful way to identify bacteria in endophthalmitis is the association of conventional cultures and panbacterial PCR on vitreous samples. Both techniques are complementary. The efficacy of these new molecular techniques should modify our future therapeutic strategies.
Chiquet
C
C
Service d'Ophtalmologie, Hôpital Michallon, Université J. Fourier, CHU de Grenoble, Grenoble, France. cchiquet@chu-grenoble.fr
Benito
Y
Y
Croize
J
J
Romanet
J-P
JP
Vandenesch
F
F
Maurin
M
M
D000208:Acute Disease; D001082:Aqueous Humor / Q000382:microbiology; D001424:Bacterial Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001431:Bacteriological Techniques; D009877:Endophthalmitis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D005051:Eubacterium / Q000235:genetics / Q000302:isolation & purification; D015807:Eye Injuries, Penetrating / Q000150:complications* / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013290:Streptococcal Infections / Q000175:diagnosis / Q000382:microbiology; D013530:Surgical Wound Infection / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D014821:Vitrectomy; D014822:Vitreous Body / Q000382:microbiology