Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes.
Hydroperoxide lyase activity was found in sugar beet leaves. Its optimum pH and temperature were, respectively, 6.7 and 22 degrees C. Under these conditions, conversion of linolenic acid 13-hydroperoxide to cis-3-hexenal with a maximum yield of 80% was reached after only 2 min. The stability of cis-3-hexenal was improved by acidifying the reaction medium. Based on these studies, a bioprocess producing green-note aldehydes in a laboratory-scale was achieved.
Rabetafika
Holy N
HN
General and Organic Chemistry Unit, Gembloux Agricultural University, 5030, Gembloux, Belgium. rabetafika.h@fsagx.ac.be
Gigot
Cédric
C
Fauconnier
Marie-Laure
ML
Ongena
Marc
M
Destain
Jacqueline
J
du Jardin
Patrick
P
Wathelet
Jean-Paul
JP
Thonart
Philippe
P
D000446:Aldehyde-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D027461:Beta vulgaris / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D006591:Hexobarbital / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology
D000447:Aldehydes; D008041:Linoleic Acids; D008042:Linolenic Acids; D008054:Lipid Peroxides; C025998:13-hydroperoxylinolenic acid; D003577:Cytochrome P-450 Enzyme System; D006591:Hexobarbital; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase; C032890:13-hydroperoxylinoleic acid