Restriction-mediated differential display (RMDD).
A variation on differential display, restriction-mediated differential display (RMDD) presents an alternative approach to the fragment display technologies. It touts high sensitivity (detection of mRNAs at a dilution of 1:100,000 and of regulation factors lower than two-fold), a strategy for avoiding false positives, nonradioactive detection, and universal applicability to any polyadenylated RNA.
Fischer
A
A
F. Hoffmann-La Roche AG, Basel, Switzerland.
D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D009580:Nitroblue Tetrazolium; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
D018076:DNA, Complementary; D012333:RNA, Messenger; D009580:Nitroblue Tetrazolium