Effects of long-term dietary intake of magnesium on rat liver transcriptome.
In the present study we investigated the effect of a two-year treatment period with a diet containing 3.2g, 0.8 g and 0.15 g Mg/kg, on the rat liver transcriptome. At the end of the study, a treatment-dependent decrease in plasmatic Mg concentration was found (0.86 +/- 0.02 mmol/L, 0.70 +/- 0.02 mmol/L and 0.52 +/- 0.03 mmol/L for groups receiving 3.2g, 0.8 g and 0.15 g Mg/kg diet, respectively). No significant treatment-related effect on body and liver weights was observed, however a dietary Mg intake-dependent increase in mortality rate occurred in animals (11%, 25% and 38% death of animals). Mg content in the diet affected gene expression in rat livers, as assessed by rat specific DNA microarrays. We identified 11 genes up-regulated and 39 genes down-regulated by at least two-fold by a decrease in Mg content and grouped them within five functional pathways: metabolism 20%, cytoarchitecture (connective tissue/cell adhesion/cytoskeleton) 12%, channels/ transporters 20%, turn-over (nucleic acid and protein) 16%, and homeostasis (stress/DNA damage/apoptosis/ageing) 32%. The results of the present study confirm the pleiotropic effects of Mg and provide further evidence that a Mg decrease in the diet may be considered as a promoting factor for pathologies, especially in the liver, during ageing.
Martin
Hélène
H
Laboratoire de biologie cellulaire, UFR des Sciences médicales et pharmaceutiques, EA 3921 OMC, Besançon, France. helene.martin@univ-fcomte.fr
Staedtler
Frank
F
Lamboley
Christelle
C
Adrian
Markus
M
Schumacher
Martin M
MM
Chibout
Salah-Dine
SD
Laurant
Pascal
P
Richert
Lysiane
L
Berthelot
Alain
A
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D020869:Gene Expression Profiling; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008274:Magnesium / Q000008:administration & dosage* / Q000097:blood / Q000494:pharmacology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D008274:Magnesium