Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis.
Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A(1) activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.
Hyun
Youbong
Y
National Research Laboratory of Plant Developmental Genetics, Department of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
Choi
Sungwook
S
Hwang
Hyun-Ju
HJ
Yu
Jihyeon
J
Nam
Sang-Jip
SJ
Ko
Jaeyoung
J
Park
Ju-Young
JY
Seo
Young Sam
YS
Kim
Eun Yu
EY
Ryu
Stephen Beungtae
SB
Kim
Woo Taek
WT
Lee
Yong-Hwan
YH
Kang
Heonjoong
H
Lee
Ilha
I
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002736:Chloroplasts / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D014644:Genetic Variation; D009928:Organ Specificity; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010739:Phospholipase D / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054466:Phospholipases A1 / Q000378:metabolism; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000648:ultrastructure; D015533:Transcriptional Activation / Q000235:genetics
D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D002265:Carboxylic Ester Hydrolases; C019262:galactolipase; C445094:DAD1 protein, Arabidopsis; D010741:Phospholipases A; D054466:Phospholipases A1; D010739:Phospholipase D