title: Characterization of ion exchange stationary phases via pH transition profiles.
abstract: New non-destructive method for characterization of ion exchange chromatographic columns based on transient pH formed by a step change in ionic strength of buffer solutions was examined. The method was used to distinguish between cation and anion or weak and strong ion exchange chromatographic supports and to determine the capacity of the chromatographic resins. The general scheme to distinguish between most commonly used types of ion exchange chromatographic columns was proposed. The duration of pH transient was shown to be linearly proportional to the total ionic capacity and was used to estimate protein dynamic binding capacity of the resin. The effects of pH, concentration and temperature on transient pH duration were examined.
authors.affiliation: BIA Separations d.o.o., Teslova 30, Ljubljana, Slovenia.
authors.forename: Nika
authors.initials: N
authors.lastname: Lendero
authors.forename: Jana
authors.initials: J
authors.lastname: Vidic
authors.forename: Peter
authors.initials: P
authors.lastname: Brne
authors.forename: Vida
authors.initials: V
authors.lastname: Frankovic
authors.forename: Ales
authors.initials: A
authors.lastname: Strancar
authors.forename: Ales
authors.initials: A
authors.lastname: Podgornik
mesh_terms: D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D007475:Ion Exchange Resins; D014867:Water / Q000737:chemistry
chemical_list: D007475:Ion Exchange Resins; D014867:Water