title: Generation of transgenic quail through germ cell-mediated germline transmission.
abstract: Here, we describe the production of transgenic quail via a germline transmission system using postmigratory gonadal primordial germ cells (gPGCs). gPGCs retrieved from the embryonic gonads of 5-day-old birds were transduced with a lentiviral vector and subsequently transferred into recipient embryos. Testcross and genetic analyses revealed that among three germline chimeric G0 quail, one male produced transgenic offspring; of 310 hatchlings from the transgenic germline chimera, 24 were identified as donor-derived offspring, and 6 were transgenic (6/310, 1.9%). Conventional transgenesis using stage X blastodermal embryos was also conducted, but the efficiency of transgenesis was similar between the two systems (<1.6 vs. 1.9% for the conventional and gPGC-mediated systems, respectively). However, substantial advantages can be gained from gPGC-mediated method in that it enables an induced germline modification, whereas direct retroviral transfer to stage X embryos causes mosaic integration. The use of gonadal PGCs for transgenesis may lead to the production of bioreactors.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Korea.
authors.forename: Sang Su
authors.initials: SS
authors.lastname: Shin
authors.forename: Tae Min
authors.initials: TM
authors.lastname: Kim
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Tae Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Hee Won
authors.initials: HW
authors.lastname: Seo
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Lee
authors.forename: Se Chang
authors.initials: SC
authors.lastname: Kwon
authors.forename: Gwan Sun
authors.initials: GS
authors.lastname: Lee
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001756:Blastoderm / Q000502:physiology; D003370:Coturnix / Q000235:genetics / Q000502:physiology*; D003433:Crosses, Genetic; D004625:Embryo, Nonmammalian / Q000502:physiology; D005241:Feathers / Q000502:physiology; D005260:Female; D005854:Germ Cells / Q000502:physiology; D018095:Germ-Line Mutation; D008297:Male; D010063:Ovum / Q000502:physiology