Mitochondria are involved in stress response of A549 alveolar cells to halothane toxicity.
During inhalation anaesthesia lung epithelial cells are directly exposed to halogenated hydrocarbons such as halothane. Information about the effects of volatile anaesthetics on lung cells is rather limited although their noxious effect on the A549 alveolar cells has been shown recently. The present study indicated that halothane decreases cell viability, impairs DNA integrity and provokes stress-induced apoptosis in A549 cells when applied at clinically relevant concentrations. Data obtained clearly demonstrated intensive expression of anti-apoptotic Bcl-2 protein during treatment with all tested concentrations. In post-treatment periods the increased DNA injury was accompanied by reduction of Bcl-2 expression. We concluded that the in vitro effect of halothane on lung cells involved alteration in the expression of proteins of the mitochondrial apoptotic pathway.
Stephanova
Elena
E
Department of Cytology, Histology and Embryology, Faculty of Biology, University of Sofia St. Kliment Ohridski, 8 Dragan Tzankov Str., 1164 Sofia, Bulgaria. stephanova@biofac.uni-sofia.bg
Topouzova-Hristova
Tanya
T
Konakchieva
Rossitza
R
D018685:Anesthetics, Inhalation / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D019254:Genes, bcl-2 / Q000187:drug effects; D006221:Halothane / Q000633:toxicity*; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D011650:Pulmonary Alveoli / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological / Q000139:chemically induced / Q000503:physiopathology*
D018685:Anesthetics, Inhalation; D006221:Halothane