ChIP-chip for genome-wide analysis of protein binding in mammalian cells.
ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. UNIT 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells.
Kim
Tae Hoon
TH
Yale University School of Medicine, New Haven, Connecticut, USA.
Barrera
Leah O
LO
Ren
Bing
B
D000818:Animals; D002460:Cell Line; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D023281:Genomics / Q000379:methods; D006801:Humans; D008322:Mammals / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics / Q000379:methods
D011506:Proteins; D004247:DNA