title: Early postnatal stress alters the extinction of context-dependent conditioned fear in adult rats.
abstract: Fear extinction is hypothesized to be a learning process based on a new inhibitory memory. The present study was conducted to elucidate the effect of early postnatal stress on the extinction of context-dependent fear memory in adult rats, with a focus on the serotonergic system. Extinction was estimated by the expression of freezing behavior during repeated extinction trials (i.e., repeated exposure to contextual fear conditioning) on consecutive days. The decrease in fear expression was attenuated in adult rats that had been subjected to footshock (FS) at the third postnatal week (3wFS), but not in those exposed to footshock at the second postnatal week (2wFS). The decreased attenuation of freezing behavior observed in 3wFS was abolished by repeated treatment with the partial N-methyl-D-aspartate receptor agonist D-cycloserine (15 mg/kg, i.p., for 4 days), which has been shown to facilitate cue-dependent extinction. Repeated treatment with the serotonin 5-hydroxytryptamine-1A (5-HT(1A)) receptor agonist tandospirone (1 mg/kg, i.p., for 4 days) prevented the expression of freezing behavior in 3wFS, whereas diazepam treatment (1 mg/kg, i.p., for 4 days) in 3wFS did not. These results suggest that exposure to early postnatal stress at the third week is responsible for attenuating extinction of contextual fear conditioning and is mediated by a serotonergic 5-HT(1A) receptor mechanism. In other words, exposure to traumatic events during the early postnatal period might precipitate long-lasting alterations in synaptic function that underlie extinction processes of context-dependent fear memory.
authors.affiliation: Department of Pharmacological Science, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. mbird@hoku-iryo-u.ac.jp
authors.forename: Machiko
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Hiroko
authors.initials: H
authors.lastname: Togashi
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Konno
authors.forename: Hiroyo
authors.initials: H
authors.lastname: Koseki
authors.forename: Riki
authors.initials: R
authors.lastname: Hirata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Izumi
authors.forename: Taku
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yoshioka
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D003213:Conditioning, Psychological; D003523:Cycloserine / Q000494:pharmacology; D005108:Extinction, Psychological; D005239:Fear; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D044282:Receptor, Serotonin, 5-HT1A / Q000502:physiology; D013315:Stress, Psychological / Q000523:psychology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D014151:Anti-Anxiety Agents; D044282:Receptor, Serotonin, 5-HT1A; D005680:gamma-Aminobutyric Acid; D003523:Cycloserine