Dynamic adsorption and characterization of phospholipid and mixed phospholipid/protein layers at liquid/liquid interfaces.
Drop profile analysis tensiometry is applied to study the adsorption dynamics of phospholipids, proteins and phospholipid/protein mixtures at liquid/liquid interfaces. Measurements of the dynamic interfacial tension of phospholipid layers give information on the adsorption mechanism and the structure of the adsorption layer. The equilibrium and dynamic adsorption of pure protein solutions, i.e. human serum album (HSA), beta-lactoglobulin (beta-LG), beta-casein (beta-CA), can be explained well by the thermodynamic model of Frumkin and the diffusion-controlled adsorption theory. The adsorption behavior from mixed phospholipid/protein solutions was also investigated in terms of dynamic interfacial tensions. Interestingly, a "skin-like" folded film of pure protein or phospholipid/protein complex layers can be observed at curved surfaces at the water/oil interfaces. The addition of phospholipids accelerates the formation of the folded structure at the drop surface through co-adsorption of proteins.
He
Qiang
Q
Beijing National Laboratory for Molecular Sciences (BNLMS), International Joint Lab, CAS Key Lab of Colloid and Interface Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Yi
Y
Lu
Gang
G
Miller
Reinhard
R
Möhwald
Helmuth
H
Li
Junbai
J
D000327:Adsorption; D000818:Animals; D002364:Caseins / Q000737:chemistry; D002417:Cattle; D006801:Humans; D007700:Kinetics; D007782:Lactoglobulins / Q000737:chemistry; D009821:Oils / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011312:Pressure; D011506:Proteins / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D002364:Caseins; D007782:Lactoglobulins; D009821:Oils; D010743:Phospholipids; D011506:Proteins; D012709:Serum Albumin; D014867:Water