title: Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA.
abstract: Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning -153 to -138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.
authors.affiliation: Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya-shi, Aichi 464-8601, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Endo
authors.forename: Misako
authors.initials: M
authors.lastname: Yokoyama
authors.forename: Munenori
authors.initials: M
authors.lastname: Morimoto
authors.forename: Kengo
authors.initials: K
authors.lastname: Shirai
authors.forename: Go
authors.initials: G
authors.lastname: Chikamatsu
authors.forename: Naoki
authors.initials: N
authors.lastname: Kato
authors.forename: Norihiro
authors.initials: N
authors.lastname: Tsukagoshi
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology*; D001483:Base Sequence; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism; D017931:DNA Primers; D004271:DNA, Fungal; D004790:Enzyme Induction; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D014990:Xylans / Q000378:metabolism
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D014990:Xylans; D002482:Cellulose; D002480:Cellulase