Plating lambda phage to generate plaques.
This unit provides detailed protocols for isolating a single plaque by titering serial dilutions or streaking on a lawn of cells. A discussion of phage transfection and in vitro packaging is also included.
Lech
K
K
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Brent
R
R
D010582:Bacteriophage lambda / Q000254:growth & development*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000821:virology*; D008967:Molecular Biology / Q000379:methods*; D010948:Viral Plaque Assay / Q000379:methods*