Plastid targeting strategies for cyanophycin synthetase to achieve high-level polymer accumulation in Nicotiana tabacum.
The production of biodegradable polymers in transgenic plants is an important challenge in plant biotechnology; nevertheless, it is often accompanied by reduced plant fitness. In order to decrease the phenotypic abnormalities caused by cytosolic production of the biodegradable polymer cyanophycin, and to increase polymer accumulation, four translocation pathway signal sequences for import into chloroplasts were individually fused to the coding region of the cyanophycin synthetase gene (cphA(Te)) of Thermosynechococcus elongatus BP-1, resulting in the constructs pRieske-cphA(Te), pCP24-cphA(Te), pFNR-cphA(Te) and pPsbY-cphA(Te). These constructs were expressed in Nicotiana tabacum var. Petit Havana SRI under the control of the constitutive cauliflower mosaic virus (CaMV) 35S promoter. Three of the four constructs led to polymer production. However, only the construct pPsbY-cphA(Te) led to cyanophycin accumulation exclusively in chloroplasts. In plants transformed with the pCP24-cphA(Te) and pFNR-cphA(Te) constructs, water-soluble and water-insoluble forms of cyanophycin were only located in the cytoplasm, which resulted in phenotypic changes similar to those observed in plants transformed with constructs lacking a targeting sequence. The plants transformed with pPsbY-cphA(Te) produced predominantly the water-insoluble form of cyanophycin. The polymer accumulated to up to 1.7% of dry matter in primary (T(0)) transformants. Specific T(2) plants produced 6.8% of dry weight as cyanophycin, which is more than five-fold higher than the previously published value. Although all lines tested were fertile, the progeny of the highest cyanophycin-producing line showed reduced seed production compared with control plants.
Hühns
Maja
M
Agrobiotechnology, University of Rostock, Justus-von-Liebig-Weg 8, 18059 Rostock, Germany.
Neumann
Katrin
K
Hausmann
Tina
T
Ziegler
Karl
K
Klemke
Friederike
F
Kahmann
Uwe
U
Staiger
Dorothee
D
Lockau
Wolfgang
W
Pistorius
Elfriede K
EK
Broer
Inge
I
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001704:Biopolymers / Q000096:biosynthesis / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000648:ultrastructure; D010940:Plant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D018517:Plant Roots / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D018087:Plastids / Q000235:genetics / Q000378:metabolism*; D012098:Reproduction; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D001704:Biopolymers; D010940:Plant Proteins; C024250:cyanophycin; D010453:Peptide Synthases; C113257:cyanophycin synthase, bacteria