title: The occurrence of HR1b in the venom of the snake Okinawa habu (Protobothrops flavoviridis).
abstract: This study describes the first isolation of hemorrhagic metalloproteinase HR1b from the venom of Okinawa habu, and its cDNA cloning. The substrate specificity of isolated HR1b definitely differed from that of HR1a, further supporting the presence of a metalloproteinase distinguishable from HR1a in the venom of Okinawa habu. The deduced amino acid sequence of HR1b showed 99.67% identity with HR1b of Amami habu, with only two amino acid residue replacements.
authors.affiliation: United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan. morinenb@pref.okinawa.lg.jp
authors.forename: Nobuya
authors.initials: N
authors.lastname: Morine
authors.forename: Seiko
authors.initials: S
authors.lastname: Matsuda
authors.forename: Koki
authors.initials: K
authors.lastname: Terada
authors.forename: Hironori
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Oku
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017837:Bothrops; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000201:enzymology*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D045726:Metalloproteases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data
chemical_list: D003435:Crotalid Venoms; D017931:DNA Primers; D045726:Metalloproteases