title: [Cytotoxicity of MTA, Dycal and GIC on human periodontal ligament cells in vitro].
abstract: OBJECTIVE
  To compare the cytotoxicity of three endodontic materials (MTA, Dycal and GIC) on human periodontal ligament cells (HPDLCs) in vitro.
  
  
  METHODS
  HPDLCs proliferation was evaluated using methylthiazol tetrazolium (MTT) assay for three endodontic materials in different concentration. SAS6.12 software package was used to analyse the results.
  
  
  RESULTS
  There was no statistically significant difference in three subgroups of MTA group (P>0.05). Statistically significant differences were found in Dycal group (P<0.05). In GIC group, there was no statistically significant difference between the control and 10 mg/ml subgroup (P>0.05). At the same time, statistically significant differences were found in between the control and 20 mg/ml or 40 mg/ml subgroup (P<0.05).
  
  
  CONCLUSIONS
  Dycal and GIC are more cytotoxic than MTA. MTA is superior to Dycal and GIC in biocompatibility.
authors.affiliation: Department of Operative Dentistry & Endodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Lie
authors.initials: L
authors.lastname: Xia
authors.forename: Long
authors.initials: L
authors.lastname: Jiang
authors.forename: Ya-qin
authors.initials: YQ
authors.lastname: Zhu
mesh_terms: D002126:Calcium Hydroxide / Q000633:toxicity*; D049109:Cell Proliferation; D005971:Glutamates / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives* / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D008903:Minerals / Q000633:toxicity*; D000068437:Pemetrexed; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects; D013778:Tetrazolium Salts
chemical_list: D005971:Glutamates; D008903:Minerals; D013778:Tetrazolium Salts; D000068437:Pemetrexed; C025702:Dycal; D006147:Guanine; D002126:Calcium Hydroxide