Marked differences in differentiation propensity among human embryonic stem cell lines.
The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.
Osafune
Kenji
K
Harvard Stem Cell Institute, 42 Church Street, Cambridge, Massachusetts 02138, USA.
Caron
Leslie
L
Borowiak
Malgorzata
M
Martinez
Rita J
RJ
Fitz-Gerald
Claire S
CS
Sato
Yasunori
Y
Cowan
Chad A
CA
Chien
Kenneth R
KR
Melton
Douglas A
DA
D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans