Freezing and recovery of hybridoma cell lines.
Liquid nitrogen storage is the method of choice for long-term safekeeping of hybridoma cell lines. Frozen aliquots of originally isolated hybridomas provide insurance against loss of antibody production and vigor during culture. There are many variations of cell freezing methods in use. However, for freezing and recovering hybridomas and lymphoid cells in general, the protocol described in this unit is simple and has been successful.
Fuller
S A
SA
Allelix Inc., Mississauga, Ontario, Canada.
Takahashi
M
M
Hurrell
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002460:Cell Line; D015925:Cryopreservation / Q000379:methods*; D005615:Freezing; D006825:Hybridomas / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal