Antiplatelet aggregation coumarins from the leaves of Murraya omphalocarpa.
Using a bioactivity-guided fractionation method, two coumarins: minumicroline acetonide (1) and epimurpaniculol senecioate (2), were isolated from the leaves of Murraya omphalocarpa Hayata (Rutaceae). Compound 1 had been previously synthesized and was now isolated from natural sources for the first time, and compound 2, possessing a negative optical rotation value, is new. The structures and their stereochemistry were fully elucidated on the basis of spectroscopic and X-ray crystallographic techniques. Both compounds 1 and 2 are active in the antiplatelet aggregation assay. Interestingly, the possible acetonide artifact 1 displayed significant antiplatelet aggregation induced not only by AA and collagen but also by platelet activating factor (PAF).
Chia
Yi-Chen
YC
Department of Food Science and Technology, Ta-Jen University, Ping Tung Hsien, Taiwan.
Chang
Fang-Rong
FR
Wang
Jinn-Chyi
JC
Wu
Chin-Chung
CC
Chiang
Michael Y-N
MY
Lan
Yu-Hsuan
YH
Chen
Keh-Shaw
KS
Wu
Yang-Chang
YC
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D008958:Models, Molecular; D032101:Murraya / Q000737:chemistry* / Q000187:drug effects; D018515:Plant Leaves / Q000737:chemistry* / Q000187:drug effects; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D011817:Rabbits; D013917:Thrombin / Q000494:pharmacology; D014961:X-Ray Diffraction
D003374:Coumarins; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D016718:Arachidonic Acid; D003094:Collagen; D013917:Thrombin