title: A highly efficient construction of GM1 epitope tetrasaccharide and its conjugation with KLH.
abstract: GM1 epitope tetrasaccharide was synthesized by a condensation of sialyl-alpha(2-3)-gal acceptor and gal-beta(1-3)-GalN donor in a highly efficient manner. After introduction of mercaptohexanol to the tetrasaccharide, it was coupled to maleimide-activated KLH carrier protein to give the desired GM1 epitope-KLH conjugate.
authors.affiliation: Department of Applied Bio-organic Chemistry, Gifu University, Gifu 501-1193, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kato
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Yuki
authors.forename: Tomio
authors.initials: T
authors.lastname: Yabe
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ishida
authors.forename: Makoto
authors.initials: M
authors.lastname: Kiso
mesh_terms: D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006433:Hemocyanins / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D008070:Lipopolysaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology
chemical_list: D000939:Epitopes; D008070:Lipopolysaccharides; D009844:Oligosaccharides; C023023:lipid-linked oligosaccharides; C037563:G(M1)-oligosaccharide; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin