AtGRX4, an Arabidopsis chloroplastic monothiol glutaredoxin, is able to suppress yeast grx5 mutant phenotypes and respond to oxidative stress.
Arabidopsis monothiol glutaredoxin (Grx), AtGRX4, was targeted to chloroplasts/plastids and had high similarity to yeast Grx5. In yeast expression assays, AtGRX4 localized to the mitochondria and suppressed the sensitivity of grx5 cells to oxidants. In addition, AtGRX4 reduced iron accumulation and rescued the lysine auxotrophy of grx5 cells. In planta, AtGRX4 RNA transcripts accumulated in growing tissues. Furthermore, AtGRX4expression was altered under various stresses. Genetic analysis revealed that seedlings of atgrx4 mutants were sensitive to oxidants. Taken together, these results suggest that AtGRX4 may have important functions in plant growth and development under extreme environments.
Cheng
Ning-Hui
NH
Plant Physiology Group, USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, 1100 Bates Street, Houston, TX 77030, USA. ncheng@bcm.tmc.edu
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D002736:Chloroplasts / Q000201:enzymology / Q000235:genetics*; D005816:Genetic Complementation Test; D054477:Glutaredoxins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008239:Lysine / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009154:Mutation; D018384:Oxidative Stress / Q000235:genetics*; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013489:Suppression, Genetic
D029681:Arabidopsis Proteins; C528771:GRX4 protein, Arabidopsis; D054477:Glutaredoxins; C528773:Grx5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide; D008239:Lysine