Dissemination of bacteria labeled with technetium-99m after laparotomy and abdominal insufflation with different CO2 pressures on rats.
OBJECTIVE
To asses the dissemination of bacteria labeled with technetium-99m (99mTc) from peritoneal cavity after different surgical procedures.


METHODS
Bacteria of the Escherichia coli species labeled with 99mTc were used in a concentration of 10(8) units of colony-makers for ml (UFC/ml) and 1 ml was inoculated through intra-peritoneal via. Forty-eight rats were divided into four groups: control, laparotomy, pneumoperitoneum with 10 mm Hg and pneumoperitoneum with 20 mm Hg of CO2. Procedures were performed 20 min after injection of the inoculum and lasted 30 min. Animals were sacrificed after six hours (Group 1) and 24 hours (Group 2). Samples of blood, liver and spleen were collected for radioactivity counting.


RESULTS
After six hours, indirect detection of the bacteria in different organs was uniform in all groups. After 24 hours, a larger detection of technetium was observed in the livers of animals of the group insufflated with 20 mm Hg of CO2, when compared with those of control group (p<0.01). The other groups did not present statistically significant variations.


CONCLUSIONS
The use of a higher intra-abdominal pressure was associated with a higher bacterial dissemination to the liver. The application of lower intra-abdominal pressures may be associated with a lower dissemination of the infectious status during laparoscopic approach of peritonitis status.
Pitombo
Marcos Bettini
MB
Medical Sciences School, UERJ, Rio de Janeiro, Brazil. mpitombo@urbi.com.br
Faria
Clarice Abreu Dos Santos Albuquerque de
CA
Bernardo
Luciana Camargo
LC
Steinbruck
Klaus
K
Bernardo Filho
Mario
M
D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D004927:Escherichia coli Infections / Q000000981:diagnostic imaging* / Q000382:microbiology; D007327:Insufflation / Q000009:adverse effects; D010535:Laparoscopy / Q000009:adverse effects; D007813:Laparotomy / Q000009:adverse effects; D008099:Liver / Q000382:microbiology / Q000528:radiation effects; D008297:Male; D010529:Peritoneal Cavity / Q000382:microbiology; D010538:Peritonitis / Q000000981:diagnostic imaging / Q000382:microbiology / Q000601:surgery*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects* / Q000379:methods; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000000981:diagnostic imaging* / Q000382:microbiology; D013154:Spleen / Q000382:microbiology / Q000528:radiation effects; D013667:Technetium
D013667:Technetium