Microscopic measurement of circular dichroism spectra.
A microscope device to measure the circular dichroism (CD) spectra of a specified microscopic region of chiral samples was constructed by combining of a couple of objective lenses and a CCD camera, which was installed in a sample chamber of a commercially available CD spectropolarimeter. By using this apparatus, high quality micro-CD spectra in the 60 x 60 microm region of samples could be measured. Micro-CD spectra of thin film of chiral DNA samples on glass and a natural kidney bean leaf were measured, and the potential of the micro-CD apparatus was successfully demonstrated.
Matsugaki
Aira
A
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan.
Takechi
Hideaki
H
Monjushiro
Hideaki
H
Watarai
Hitoshi
H
D002942:Circular Dichroism / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004867:Equipment Design; D008567:Membranes, Artificial; D008859:Microscopy, Polarization / Q000295:instrumentation; D010316:Particle Size; D027805:Phaseolus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012680:Sensitivity and Specificity
D008567:Membranes, Artificial; D004247:DNA