Conjugation of (E)-5-[2-(Methoxycarbonyl)ethenyl]cytidine to hydrophilic microspheres: development of a mobile microscale UV light actinometer.
( E)-5-[2-(Methoxycarbonyl)ethenyl]cytidine was biotinylated through a diisopropylsilylacetal linkage and attached to the surface of hydrophilic streptavidin-coated microspheres through the high-affinity noncovalent interaction between biotin and streptavidin. The functionalized microspheres form a stable suspension in water. Upon UV irradiation, the nonfluorescent ( E)-5-[2-(methoxycarbonyl)ethenyl]cytidine on the microspheres undergoes photocyclization to produce highly fluorescent 3-beta-D-ribofuranosyl-2,7-dioxopyrido[2,3-d]pyrimidine. The fluorescence intensity of the microspheres can be correlated to the particle-specific UV doses applied at different suspension concentrations. The microspheres allow one to measure the UV dose (fluence) distribution in high-throughput water disinfection systems.
Fang
Shiyue
S
Department of Medicinal Chemistry and Molecular Pharmacology, School of Civil Engineering, Purdue University, West Lafayette, IN 47907-2051, USA.
Guan
Yousheng
Y
Blatchley
Ernest R
ER
Shen
Chengyue
C
Bergstrom
Donald E
DE
D001710:Biotin / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003562:Cytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D005453:Fluorescence; D007202:Indicators and Reagents; D008863:Microspheres; D009705:Nucleosides / Q000737:chemistry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D014466:Ultraviolet Rays
C503549:5-(2-(methoxycarbonyl)ethenyl)cytidine; D007202:Indicators and Reagents; D009705:Nucleosides; D012997:Solvents; D003562:Cytidine; D001710:Biotin; D019809:Streptavidin