title: Intracellular alpha-keto acid quantification by fluorescence-HPLC.
abstract: Procedures for the analysis of free alpha-keto acids in human fluids (i.e. plasma, cerebrospinal fluid, urine, etc.) as well as for studying the dynamic free alpha-keto acid pools in differentiated tissues and organ cells have been the subject of growing clinical interest in the study of metabolic regulatory and pathophysiological phenomena. Due to the high instability and polarity of the alpha-keto acids being examined, the development of a quantitative and reproducible analysis of metabolically relevant intracellular alpha-keto acids still presents a substantial methodological challenge. The aim of small sample size, rapid, non-damaging and "metabolism-neutral" cell isolation, careful sample preparation and stability, as well as reproducible analytics technology is not often achieved. Only few of the methods described can satisfy the rigorous demands for an ultra-sensitive, comprehensive and rapid intracellular alpha-keto acid analysis.
authors.affiliation: Dr. Ing. Herbert Knauer GmbH, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Fuchs
authors.forename: J
authors.initials: J
authors.lastname: Engel
authors.forename: M
authors.initials: M
authors.lastname: Campos
authors.forename: R
authors.initials: R
authors.lastname: Matejec
authors.forename: M
authors.initials: M
authors.lastname: Henrich
authors.forename: H
authors.initials: H
authors.lastname: Harbach
authors.forename: M
authors.initials: M
authors.lastname: Wolff
authors.forename: K
authors.initials: K
authors.lastname: Weismüller
authors.forename: T
authors.initials: T
authors.lastname: Menges
authors.forename: M C
authors.initials: MC
authors.lastname: Heidt
authors.forename: I D
authors.initials: ID
authors.lastname: Welters
authors.forename: M
authors.initials: M
authors.lastname: Krüll
authors.forename: G
authors.initials: G
authors.lastname: Hempelmann
authors.forename: J
authors.initials: J
authors.lastname: Mühling
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007651:Keto Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D007651:Keto Acids