title: DNA damage and cell necrosis induced by naphthalene due to the modulation of biotransformation enzymes in an estuarine crab Scylla serrata.
abstract: The sublethal effect of naphthalene (2.5, 5, and 10 mg L(-1)) was studied in an estuarine crab Scylla serrata with reference to macromolecular changes. Biotransformation enzymes such as cytochrome P450, cytochrome b(5), NADPH cytochrome P450 reductase, aryl hydrocarbon hydroxylase, glutathione-S-transferase, and UDP-glucuronyl transferase were elevated in the hepatopancreas of naphthalene-exposed crabs in comparison with control. Remarkable amount of DNA damage and cell necrosis was observed in hepatopancreas, hemolymph, and ovary of the crabs exposed to naphthalene, when compared with control. For all the parameters studied, a concentration-dependent gradient of the changes was observed. The expression of DNA damage and cell necrosis suggests an increased production of oxidants during naphthalene metabolism.
authors.affiliation: Department of Pharmacology and Environmental Toxicology, Dr. A. L. Mudaliar Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India. viji222@rediffmail.com
authors.forename: K
authors.initials: K
authors.lastname: Vijayavel
authors.forename: M P
authors.initials: MP
authors.lastname: Balasubramanian
mesh_terms: D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D003386:Brachyura / Q000187:drug effects* / Q000201:enzymology*; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D043143:Hepatopancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D009281:Naphthalenes / Q000633:toxicity*; D009336:Necrosis; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D009281:Naphthalenes; C031721:naphthalene