Nuclease tolerant FRET probe based on DNA-quantum dot conjugation.
We have developed a fluorescence resonance energy transfer (FRET) probe based on the conjugation of a quantum dot (QD) with dye (YOYO-3) intercalated DNA. The FRET-inducing electrostatic coupling of DNA and the QD made structural changes to the QD-DNA conjugates, which significantly prevented an enzymatic reaction between the DNA and a conventional restriction endonuclease (EcoRI).
Onoshima
Daisuke
D
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya, Japan. onoshima.daisuke@d.mbox.nagoya-u.ac.jp
Kaji
Noritada
N
Tokeshi
Manabu
M
Baba
Yoshinobu
Y
D004247:DNA / Q000737:chemistry*; D004720:Endonucleases / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D010080:Oxazoles / Q000737:chemistry*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D055672:Static Electricity
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D010080:Oxazoles; C412625:YoYo-3; D004247:DNA; D004720:Endonucleases