Rapid and sensitive on-line liquid chromatographic/tandem mass spectrometric determination of an ethylene oxide-DNA adduct, N7-(2-hydroxyethyl)guanine, in urine of nonsmokers.
Ethylene oxide (EtO) is classified as a known human carcinogen. The formation of EtO-DNA adducts is considered as an important early event in the EtO carcinogenic process. An isotope-dilution on-line solid-phase extraction and liquid chromatography coupled with tandem mass spectrometry method was then developed to analyze one of the EtO-DNA adducts, N7-(2-hydroxyethyl)guanine (N7-HEG), in urine of 46 nonsmokers with excellent accuracy, sensitivity and specificity. The merits of this method include small sample volume (only 120 microL urine required), automated sample cleanup, and short total run time (12 minutes per sample). This method demonstrates its high-throughput capacity for future molecular epidemiology studies on the potential health effects resulting from the low-dose EtO exposure.
Huang
Chih-Chun Jean
CC
Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 35 Keyan Rd., Zhunan Town, Miaoli County 35053, Taiwan.
Shih
Wei-Chung
WC
Wu
Chia-Fang
CF
Chen
Ming-Feng
MF
Chen
Yi-Lung
YL
Lin
Ying-Huei
YH
Wu
Kuen-Yuh
KY
D000393:Air Pollutants / Q000737:chemistry*; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000652:urine*; D005027:Ethylene Oxide / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D012016:Reference Values; D012907:Smoking / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000393:Air Pollutants; D015415:Biomarkers; D018736:DNA Adducts; C035018:N(7)-hydroxyethylguanine; D006147:Guanine; D004247:DNA; D005027:Ethylene Oxide