Enhanced NMR signal detection of imino protons in RNA molecules containing 3' dangling nucleotides.
We present a method for improving the quality of nuclear magnetic resonance (NMR) spectra involving exchangeable protons near the base of the stem of RNA hairpin molecules. NMR spectra of five different RNA hairpins were compared. These hairpins consisted of a native RNA structure and four molecules each having different unpaired, or dangling, nucleotides at the 3' end. NMR experiments were acquired in water for each construct and the quality of the imino proton spectral regions were examined. The imino resonances near the base of the stem of the wild type RNA structure were not observed due to breathing motions. However, a significant increase in spectral quality for molecules with dangling 3' adenosine or guanosine nucleotides was observed, with imino protons detected in these constructs that were not observed in the wild type construct. A modest improvement in spectral quality was seen for the construct with a 3' unpaired uridine, whereas no significant improvement was observed for a 3' unpaired cytidine. This improvement in NMR spectral quality mirrors the increased thermodynamic stability observed for 3' unpaired nucleotides which is dependant on the stacking interactions of these nucleotides against the base of the stem. The use of a dangling 3' adenosine nucleotide represents an easy method to significantly improve the quality of NMR spectra of RNA molecules.
Amborski
Andrew N
AN
Department of Chemistry, York University, M3J 1P3, Toronto, ON, Canada.
Johnson
Philip E
PE
D020413:3' Untranslated Regions / Q000737:chemistry* / Q000502:physiology; D001483:Base Sequence; D007097:Imines / Q000032:analysis / Q000737:chemistry*; D008969:Molecular Sequence Data; D009068:Movement; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis / Q000737:chemistry; D011522:Protons; D012313:RNA / Q000737:chemistry*; D017423:Sequence Analysis, RNA / Q000379:methods; D012815:Signal Processing, Computer-Assisted
D020413:3' Untranslated Regions; D007097:Imines; D009711:Nucleotides; D011522:Protons; D012313:RNA