S. pombe strain maintenance and media.
Fission yeast can be grown and maintained using similar culture methods to those employed for budding yeast. The optimal media for S. pombe is somewhat different than that employed for S. cerevisiae, although budding yeast media can be used if necessary. Good sterile technique is essential to prevent contamination by airborne molds or bacteria.
Forsburg
Susan L
SL
Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA.
D015925:Cryopreservation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology*; D004171:Diploidy; D007202:Indicators and Reagents; D009172:Mycology / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000254:growth & development*
D003470:Culture Media; D007202:Indicators and Reagents