Runx1 modulates developmental, but not injury-driven, hair follicle stem cell activation.
Aml1/Runx1 controls developmental aspects of several tissues, is a master regulator of blood stem cells, and plays a role in leukemia. However, it is unclear whether it functions in tissue stem cells other than blood. Here, we have investigated the role of Runx1 in mouse hair follicle stem cells by conditional ablation in epithelial cells. Runx1 disruption affects hair follicle stem cell activation, but not their maintenance, proliferation or differentiation potential. Adult mutant mice exhibit impaired de novo production of hair shafts and all temporary hair cell lineages, owing to a prolonged quiescent phase of the first hair cycle. The lag of stem cell activity is reversed by skin injury. Our work suggests a degree of functional overlap in Runx1 regulation of blood and hair follicle stem cells at an equivalent time point in the development of these two tissues.
Osorio
Karen M
KM
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA.
Lee
Song Eun
SE
McDermitt
David J
DJ
Waghmare
Sanjeev K
SK
Zhang
Ying V
YV
Woo
Hyun Nyun
HN
Tumbar
Tudorita
T
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D050676:Core Binding Factor Alpha 2 Subunit / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018390:Gene Targeting; D018859:Hair Follicle / Q000166:cytology* / Q000254:growth & development / Q000293:injuries / Q000378:metabolism*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D012038:Regeneration
D050676:Core Binding Factor Alpha 2 Subunit; C493726:Runx1 protein, mouse