Cloning of hybridoma cell lines by limiting dilution.
Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.
Fuller
S A
SA
Allelix Inc., Mississauga, Ontario.
Takahashi
M
M
Hurrell
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D019976:Cloning, Organism / Q000379:methods*; D006825:Hybridomas / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal