RNA interference in mammalian cell systems.
The use of RNA interference (RNAi) to evoke gene silencing in mammalian cells has almost become routine laboratory practice. Through refinement of double-stranded RNA (dsRNA) triggers of RNAi and creation of genome-scale libraries, the first genome-wide loss of function screens have been carried out in mammals. This review discusses some of the key features of RNAi in mammalian systems.
Paddison
Patrick J
PJ
Cold Spring Harbor Fellows Program, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11771, USA. paddison@cshl.edu
D000818:Animals; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D015723:Gene Library; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D008322:Mammals / Q000235:genetics*; D034622:RNA Interference; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism
D039642:Eukaryotic Initiation Factors; D043244:Ribonuclease III