title: Activation of protein splicing with light in yeast.
abstract: Spatiotemporal regulation of protein function is a key feature of living systems; experimental tools that provide such control are of great utility. Here we report a genetically encoded system for controlling a post-translational process, protein splicing, with light. Studies in Saccharomyces cerevisiae demonstrate that fusion of a photodimerization system from Arabidopsis thaliana to an artificially split intein permits rapid activation of protein splicing to yield a new protein product.
authors.affiliation: Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Ave., New York, New York 10065, USA.
authors.forename: Amy B
authors.initials: AB
authors.lastname: Tyszkiewicz
authors.forename: Tom W
authors.initials: TW
authors.lastname: Muir
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D019281:Dimerization; D008027:Light; D051939:Phytochrome B / Q000235:genetics; D011499:Protein Processing, Post-Translational; D019154:Protein Splicing / Q000528:radiation effects*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D051939:Phytochrome B; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C116162:PIF3 protein, Arabidopsis