title: Is meconium from healthy newborns actually sterile?
abstract: In a previous study, bacteria were able to be isolated from umbilical cord blood of healthy neonates and from murine amniotic fluid obtained by caesarean section. This suggested that term fetuses are not completely sterile and that a prenatal mother-to-child efflux of commensal bacteria may exist. Therefore, the presence of such bacteria in meconium of 21 healthy neonates was investigated. The identified isolates belonged predominantly to the genuses Enterococcus and Staphylococcus. Later, a group of pregnant mice were orally inoculated with a genetically labelled E. fecium strain previously isolated from breast milk of a healthy woman. The labelled strain could be isolated and PCR-detected from meconium of the inoculated animals obtained by caesarean section one day before the predicted date of labor. In contrast, it could not be detected in samples obtained from a non-inoculated control group.
authors.affiliation: Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Jiménez
authors.forename: María L
authors.initials: ML
authors.lastname: Marín
authors.forename: Rocío
authors.initials: R
authors.lastname: Martín
authors.forename: Juan M
authors.initials: JM
authors.lastname: Odriozola
authors.forename: Mónica
authors.initials: M
authors.lastname: Olivares
authors.forename: Jordi
authors.initials: J
authors.lastname: Xaus
authors.forename: Leonides
authors.initials: L
authors.lastname: Fernández
authors.forename: Juan M
authors.initials: JM
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D016984:Enterococcus faecium / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008431:Maternal-Fetal Exchange; D008470:Meconium / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D013030:Spain