Simultaneous investigation of the influence of topography and charge on protein adsorption using artificial nanopatterns.
The combined influence of surface topography and charge of a polymer surface on the adsorption of the protein avidin has been investigated. Atomic force microscopy contact mode imaging and charge writing were used to create defined topographical roughness and electrostatic charge patterns on the surface of polystyrene. Increased avidin adsorption was found on nanometer-size topographical patterns, but the adsorption remained unaffected by electrostatic patterns.
Blanco
E Macarena
EM
London Centre for Nanotechnology and Department of Medicine, University College London, 5 University Street, London WC1E 6JJ, United Kingdom.
Horton
Michael A
MA
Mesquida
Patrick
P
D000327:Adsorption; D001360:Avidin / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
D011137:Polystyrenes; D001360:Avidin