Effects of daidzein on messenger ribonucleic Acid expression of gonadotropin receptors in chicken ovarian follicles.
Effects of daidzein on expression of mRNA of gonadotropin receptors [follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR)] were evaluated in ovarian follicles of ISA laying hens that were 13 mo old in the postpeak period of egg laying. The hens were randomly allocated as control and daidzein-treated groups, with daidzein supplemented to the basal diet at the level of 10 mg/kg for 7 wk. The granulosa layers of preovulatory follicles (F1, F2, F3, F4, F5) and follicular layers of the small yellow follicle (SYF), large white follicle (LWF), and atretic follicle were collected. The mRNA expression of related genes was measured by semiquantitative reverse transcription PCR. Results showed that daidzein significantly increased the egg-laying rate (P < 0.05) and the number of SYF and LWF (P < 0.05). The relative abundance of the FSHR mRNA decreased in the granulosa layers from F5 to F1, but LHR mRNA displayed the opposite trend in developmental changes. Treatment with daidzein resulted in increased expression of FSHR mRNA in LWF, SYF, and granulosa layers of F4 to F2 and LHR mRNA in granulosa layers of F4 and F1 (P < 0.05). These results indicated that dietary supplementation of daidzein upregulated mRNA expression of gonadotropin receptors to improve follicle development in chicken developing follicles and laying performance after the peak laying period.
Liu
H Y
HY
College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China.
Zhang
C Q
CQ
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D010058:Oviposition; D012333:RNA, Messenger / Q000378:metabolism*; D011897:Random Allocation; D011962:Receptors, FSH / Q000235:genetics; D011967:Receptors, Gonadotropin / Q000235:genetics*; D011974:Receptors, LH / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D007529:Isoflavones; D012333:RNA, Messenger; D011962:Receptors, FSH; D011967:Receptors, Gonadotropin; D011974:Receptors, LH; C004742:daidzein