title: Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping.
abstract: Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all studied box C/D snoRNA mutants we uncovered phenotypes to extraribosomal growth inhibitors, most frequently reflected in alteration in growth lag (adaptation time). A number of strains were highly pleiotropic and displayed a great number of sensitive phenotypes, e.g., deletion mutants of snR70 and snR71, which both have clear human homologues, and deletion mutants of snR65 and snR68. Our data indicate that individual rRNA ribose methylations can play either distinct or general roles in the workings of the ribosome.
authors.affiliation: Department of Cell and Molecular Biology, Göteborg University, 405 30 Göteborg, Sweden.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Esguerra
authors.forename: Jonas
authors.initials: J
authors.lastname: Warringer
authors.forename: Anders
authors.initials: A
authors.lastname: Blomberg
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D005800:Genes, Fungal; D008745:Methylation; D009690:Nucleic Acid Conformation / Q000187:drug effects; D010641:Phenotype; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020537:RNA, Small Nucleolar / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012266:Ribose / Q000737:chemistry*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D000900:Anti-Bacterial Agents; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D020537:RNA, Small Nucleolar; D012266:Ribose