title: New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator.
abstract: In the present study, we report the comparative proteome profiles of proteins solubilized from 37 breast cancer surgical tissues, normalized for the actin content. Blood-derived proteins were excluded from the analysis. Among the tumor-derived protein spots, a large proportion (39%) was found present in all patients. These included several glycolytic enzymes, detox and heat shock proteins, members of annexin and S100 protein families, cathepsin D, and two "rare" proteins, DDAH2 involved in the angiogenesis control, and the oncogene PARK7. Other proteins, such as psoriasin, galectin1, cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression level, which suggests their possible role for patient stratification.
authors.affiliation: Dipartimento di Oncologia Sperimentale e Applicazioni Cliniche (DOSAC), Università di Palermo, Patermo, Italy. idapucci@unipa.it
authors.forename: Ida
authors.initials: I
authors.lastname: Pucci-Minafra
authors.forename: Patrizia
authors.initials: P
authors.lastname: Cancemi
authors.forename: Nadia Ninfa
authors.initials: NN
authors.lastname: Albanese
authors.forename: Gianluca
authors.initials: G
authors.lastname: Di Cara
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Marabeti
authors.forename: Antonio
authors.initials: A
authors.lastname: Marrazzo
authors.forename: Salvatore
authors.initials: S
authors.lastname: Minafra
mesh_terms: D000199:Actins / Q000032:analysis*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000199:Actins; D020543:Proteome