Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection.
To obtain insight in mechanisms of action of extracellular adenosine triphosphate (ATP) and adenosine, a simple HPLC method has been optimized and applied to investigate ATP metabolism in human whole blood ex vivo. This method provided good chromatographic resolution and peak shape for all eight compounds within a 19 min run time. The baseline was clean, the lower limit of quantification was below 0.3 micromol/L for all adenine nucleotides and the method demonstrated good linearity. Within-day precision ranged from 0.7 to 5.9% and between-days from 2.6 to 15.3%. Simplicity and simultaneous detection of ATP and its metabolites make this method suitable for clinical pharmacokinetic studies.
Coolen
Erik J C M
EJ
Department of Epidemiology, NUTRIM, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. erik.coolen@epid.unimaas.nl <erik.coolen@epid.unimaas.nl>
Arts
Ilja C W
IC
Swennen
Els L R
EL
Bast
Aalt
A
Stuart
Martien A Cohen
MA
Dagnelie
Pieter C
PC
D000227:Adenine Nucleotides / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate