title: Phase-contrast microscopy studies of early cisplatin-induced morphological changes of malignant mesothelioma cells and the correspondence to induced apoptosis.
abstract: Cisplatin treatment efficacy of malignant pleural mesothelioma (MPM) is aggravated by resistance and adverse effects. In P31 MPM cells, cisplatin induces morphological changes and apoptosis. To determine if very early (10 minutes) morphological responses corresponded to apoptosis-induction, cisplatin effects on P31 morphology were examined with phase-contrast microscopy (PCM), scanning electron microscopy (SEM), and flow cytometry (fluorescence-activated cell sorting [FACS]), and compared to apoptosis-induction over time. Increased membrane protrusions were identified with PCM and SEM, but these were not consistent with the induction of apoptosis. The authors concluded that very early morphological changes can be determined with PCM in MPM, but they did not convincingly correspond to apoptosis induction.
authors.affiliation: Department of Medical Biosciences, Clinical Chemistry, Umeå University, Umeå, Sweden.
authors.forename: Veronica
authors.initials: V
authors.lastname: Janson
authors.forename: Parviz
authors.initials: P
authors.lastname: Behnam-Motlagh
authors.forename: Roger
authors.initials: R
authors.lastname: Henriksson
authors.forename: Per
authors.initials: P
authors.lastname: Hörstedt
authors.forename: Karl Gunnar
authors.initials: KG
authors.lastname: Engström
authors.forename: Kjell
authors.initials: K
authors.lastname: Grankvist
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D020128:Inhibitory Concentration 50; D008654:Mesothelioma / Q000188:drug therapy* / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010997:Pleural Neoplasms / Q000188:drug therapy* / Q000473:pathology; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin