Development of an immunochromatographic assay for the rapid detection of bromoxynil in water.
A rapid immunochromatographic one-step strip test was developed to specifically determine bromoxynil in surface and drinking water by competitive inhibition with the nano colloidal gold-conjugated monoclonal antibody (mAb). Bromoxynil standard samples of 0.01-10 mg L(-1) in water were tested by this method and the visual limit was 0.06 mg L(-1). The assay only required 5 min and one-step by dispensing a drop of sample solution onto a strip. Parallel analysis of water samples with bromoxynil showed comparable results from one-step strip test and ELISA. Therefore, the one-step strip test is very useful as a screening method for qualitative detection of bromoxynil in water.
Zhu
Jiang
J
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Chen
Wenchao
W
Lu
Yitong
Y
Cheng
Guohua
G
D000911:Antibodies, Monoclonal; D002845:Chromatography / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D017979:Gold Colloid; D006540:Herbicides / Q000032:analysis*; D007118:Immunoassay / Q000379:methods; D009570:Nitriles / Q000032:analysis* / Q000276:immunology; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D006540:Herbicides; D009570:Nitriles; D011934:Reagent Strips; D014874:Water Pollutants, Chemical; C058457:Aurodye; C006826:bromoxynil