Interactions of acetylcholinesterase with caveolin-1 and subsequently with cytochrome c are required for apoptosome formation.
Acetylcholinesterase (AChE) is emerging as an important component in leading to apoptosis. Our previous study demonstrated that silencing of the AChE gene blocked the interaction between cytochrome c and apoptotic protease-activating factor-1 (Apaf-1) in etoposide-induced apoptosis of HT-29 cells. We undertook this study to further dissect the molecular role of AChE in apoptosome formation. The present study elicited that small interfering RNA (siRNA) to cytochrome c gene blocked the interaction of AChE with Apaf-1, whereas siRNA to Apaf-1 gene did not block the interaction of AChE with cytochrome c, indicating that the interaction of AChE with cytochrome c is required for the interaction between cytochrome c and protease-activating factor-1. We further observed that AChE is localized to caveolae via interacting with caveolin-1 during apoptosis and that the disruption of caveolae prevented apoptosome formation. These data indicate that the interactions of AChE with caveolin-1 and subsequently with cytochrome c appear to be indispensable for apoptosome formation.
Park
Sang Eun
SE
Department of Anatomy and Cell Biology, Dong-A University College of Medicine and Medical Science Research Center, Busan 602-714, South Korea.
Jeong
Seung Hun
SH
Yee
Soo-Bog
SB
Kim
Tae Hyun
TH
Soung
Young Hwa
YH
Ha
Nam Chul
NC
Kim
Nam Deuk
ND
Park
Jae-Yong
JY
Bae
Hae Rahn
HR
Park
Bong Soo
BS
Lee
Hye Jeong
HJ
Yoo
Young Hyun
YH
D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001483:Base Sequence; D051242:Caveolin 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms; D045304:Cytochromes c / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D013467:Sulindac / Q000494:pharmacology; D014162:Transfection
D051242:Caveolin 1; D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D013467:Sulindac; D045304:Cytochromes c; D000110:Acetylcholinesterase