Microalgal photosynthetic activity measurement system for rapid toxicity assessment.
A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.
Cho
Chul-Woong
CW
Department of Bioprocess Engineering, Chonbuk National University, Chonbuk, Republic of Korea.
Pham
Thi Phuong Thuy
TP
Jeon
You-Chul
YC
Min
Jiho
J
Jung
Ho Young
HY
Lee
Dae Sung
DS
Yun
Yeoung-Sang
YS
D015374:Biosensing Techniques / Q000379:methods; D001881:Borates / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D000460:Chlorophyta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D007093:Imidazoles / Q000633:toxicity*; D000432:Methanol / Q000633:toxicity*; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D018675:Toxicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*
C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D001881:Borates; D007093:Imidazoles; D014874:Water Pollutants, Chemical; D010100:Oxygen; D000432:Methanol