title: Mapping of fruit allergens by 2D electrophoresis and immunodetection.
abstract: Proteomic analyses of fruits are confronted with a series of specific obstacles: a general low protein content in plant tissues, allergen extraction from highly complex matrices and protein determination in the presence of interfering compounds. Different methods are currently being introduced to achieve higher protein yields and a simultaneous removal of interfering substances, such as polyphenols and polysaccharides. However, no universal protocol suitable for protein purification from any given plant species is available. Protein profiling by 2DE-western blotting offers a powerful tool for the detection and characterization of known and novel plant allergens. Moreover, the detection of IgE-reactive proteins from fruits is improved by combining western blot and alternative visualization techniques. The recent developments in bioinformatics and databases facilitate the interpretation of profiling studies with regard to novel potential fruit allergens.
authors.affiliation: Institute of Applied Microbiology, Plant Biotechnology Unit, Department of Biotechnology, BOKU University, Muthgasse 18, House B Vienna, A-1190, Austria. gorji.marzban@boku.ac.at
authors.forename: Gorji
authors.initials: G
authors.lastname: Marzban
authors.forename: Anita
authors.initials: A
authors.lastname: Herndl
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Maghuly
authors.forename: Hermann
authors.initials: H
authors.lastname: Katinger
authors.forename: Margit
authors.initials: M
authors.lastname: Laimer
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D052179:Antigens, Plant / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western / Q000379:methods*; D003429:Cross Reactions; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D000939:Epitopes / Q000737:chemistry; D005512:Food Hypersensitivity / Q000175:diagnosis / Q000209:etiology; D005638:Fruit / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D007457:Iodine Radioisotopes / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D011058:Pollen / Q000276:immunology; D012698:Serologic Tests / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D000939:Epitopes; D007457:Iodine Radioisotopes; D010940:Plant Proteins; D007073:Immunoglobulin E