title: The effect of hFVIII transgene on the chromosomal aneuploidy rate in rabbits.
abstract: The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no. 1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 microg/ml colcemide. No significant difference in chromosomal aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate. In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.
authors.affiliation: Slovak Agricultural Research Centre, Hlohovska 2, 949 92 Nitra, Slovak Republic.
authors.forename: Jozef
authors.initials: J
authors.lastname: Curlej
authors.forename: Vladimir
authors.initials: V
authors.lastname: Parkanyi
authors.forename: Jozef
authors.initials: J
authors.lastname: Bulla
authors.forename: Rastislav
authors.initials: R
authors.lastname: Jurcik
authors.forename: Peter
authors.initials: P
authors.lastname: Chrenek
mesh_terms: D000782:Aneuploidy; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006801:Humans; D011817:Rabbits; D013925:Thromboplastin / Q000235:genetics*; D019076:Transgenes
chemical_list: D017931:DNA Primers; D013925:Thromboplastin