Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus.
Trichomonas gallinae and Tritrichomonas foetus are flagellated parasitic protozoa of the upper digestive tract of birds and the urogenital tract of cattle, respectively. Both of these species are important in the veterinary field, due to the fact that they cause significant economic losses. Therefore, we investigated the morphology of these parasites by studying microtubule cytoskeleton organization. FLUTAX-2, an active fluorescent derivative of Taxol, was used in this study. This fluorescent taxoid binds to polymerized alphabeta-tubulin dimers. Our results showed that FLUTAX-2 was able to bind to and stabilize microtubules of intact T. gallinae and T. foetus trophozoites, allowing the microtubular cytoskeleton to be easily observed by fluorescence microscopy. T. foetus and T. gallinae had no differences in their FLUTAX-2 binding profiles. Further studies may allow this technique to be improved, and it may possibly be used as a routine laboratory method for the diagnosis of avian and bovine trichomonosis.
Vieira
P B
PB
Departamento de Análises, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul. Avenida Ipiranga, 2752, 90610-000 Porto Alegre, RS, Brazil.
Borges
F P
FP
Gottardi
B
B
Stuepp
C
C
Larré
A B
AB
Tasca
T
T
De Carli
G A
GA
D000818:Animals; D002417:Cattle; D010856:Columbidae; D003599:Cytoskeleton / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure*; D043823:Taxoids / Q000378:metabolism; D014244:Trichomonas / Q000648:ultrastructure*; D016835:Tritrichomonas foetus / Q000648:ultrastructure*
D005456:Fluorescent Dyes; C413450:Flutax 2; D043823:Taxoids