[Application of spindle-view in the enucleation porcine of oocytes].
The Spindle-view, a specialized instrument for observing spindle image, was applied to observe the meiotic spindles of vitro matured porcine oocytes at 36, 42, 44, 48h, and enucleation from porcine, comparing to the previously methods (McGrath-Solter's method and two-step-squeezing method) in the enucleated. The results showed that: (1) there was no noticeable differences at vicinity of spindle images and 1st polar body among in vitro matured porcine oocytes at 40-48 h under the instrument; (2) Spindle-view is suitable for the observation of meiotic spindles of matured oocytes and enucleation from porcine; the modified Spindle-view method for enucleation is significantly better than McGrath-Solter' s method and two-step-squeezing method in the enucleated rates (95.5%, 42.1%, 74.2%, P < 0.0l) of absolutely removing nuclei matter; (3) the spindle images could be used to monitor the oocyte qualities.
Yang
Yu
Y
Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Dai
Jian-Jun
JJ
Zhang
Ting-Yu
TY
Wu
Hua-Li
HL
Chen
Xue-Jin
XJ
Zhang
De-Fu
DF
Ma
Heng-Dong
HD
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D003584:Cytological Techniques; D005260:Female; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D008941:Spindle Apparatus / Q000648:ultrastructure*; D013552:Swine