[Two-dimensional gel electrophoresis profiles of human normal prostate cells successfully established by 2-DE coupled with laser capture microdissection].
OBJECTIVE
To establish a technological system of proteomics research for displaying the full-scale protein expression spectrum of the prostate cells.


METHODS
We obtained the epithelial cells from normal prostate tissues by laser capture microdissection (LCM) technology, extracted the proteins from them and established two-dimensional gel electrophoresis (2-DE) profiles of the proteins by immobilized pH gradient (IPG) two-dimensional electrophoresis. Then protein spot detection and matching were performed with the scanner and the ImageMaster 2D Elite analysis software. With the help of the Ettanspotter and digester, protein spots that matched well across the gels were extracted, digested and further identified by assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).


RESULTS
The 2-DE profiles of prostate cells were established, and one protein spot from normal prostate cells identified successfully.


CONCLUSIONS
The 2-DE profiles of normal prostate cells have good reproducibility. We have tentatively established the proteomics research technology of normal prostate cells and demonstrated the feasibility of proteomics research with the model of human normal prostate cells.
Li
Qun
Q
Key Laboratory of Pathobiology, Ministry of Education, Basic Medical School, Jilin 130021, China.
Li
Yi-Lei
YL
Yang
Xin-Yu
XY
Zhang
Hai-Ying
HY
Li
Hui-Min
HM
Li
Yu-Lin
YL
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007834:Lasers; D008297:Male; D042282:Microdissection / Q000379:methods; D008875:Middle Aged; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
D020543:Proteome