title: Microalgal photosynthetic activity measurement system for rapid toxicity assessment.
abstract: A systematic biosensor is constructed for the estimation of toxic compounds based on photosynthetic activity measurement in Selenastrum capricornutum cells. The photosynthetic response was evaluated as a function of light intensity, cell concentration and initial dissolved oxygen. The inhibitory effect of some toxicants (1-butyl-3-methylimidazolium tetrafluoroborate, methanol) on dissolved oxygen production was also determined. In all cases, a toxic response was detected (i.e. a dose-related inhibition of photosynthetic activity was observed). For the present system, a time of only 2 h was needed to predict EC50 values as compared to 96 h for a conventional algal assay based on algal growth rate. Thus, the developed biosensor was proved to be useful as a rapid and simple test method in environmental toxicity assessment.
authors.affiliation: Department of Bioprocess Engineering, Chonbuk National University, Chonbuk, Republic of Korea.
authors.forename: Chul-Woong
authors.initials: CW
authors.lastname: Cho
authors.forename: Thi Phuong Thuy
authors.initials: TP
authors.lastname: Pham
authors.forename: You-Chul
authors.initials: YC
authors.lastname: Jeon
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.forename: Ho Young
authors.initials: HY
authors.lastname: Jung
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Yeoung-Sang
authors.initials: YS
authors.lastname: Yun
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D001881:Borates / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D000460:Chlorophyta / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring; D007093:Imidazoles / Q000633:toxicity*; D000432:Methanol / Q000633:toxicity*; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D018675:Toxicity Tests / Q000379:methods; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D001881:Borates; D007093:Imidazoles; D014874:Water Pollutants, Chemical; D010100:Oxygen; D000432:Methanol