title: Enhanced subtyping scheme for Salmonella enteritidis.
abstract: To improve pulsed-field gel electrophoresis-based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis.
authors.affiliation: University of Maryland, College Park, Maryland, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.forename: Christine E
authors.initials: CE
authors.lastname: Keys
authors.forename: Shaohua
authors.initials: S
authors.lastname: Zhao
authors.forename: Jianghong
authors.initials: J
authors.lastname: Meng
authors.forename: Eric W
authors.initials: EW
authors.lastname: Brown
mesh_terms: D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D010802:Phylogeny; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics