Immobilized proanthocyanidins for the capture of bacterial lipopolysaccharides.
Proanthocyanidins (PACs) are an abundant class of compounds found in a variety of plant materials. Here we demonstrate the application of these materials as capture molecules for the removal of bacterial lipopolysaccharide (LPS) from solution. PACs from whole cranberries, grape juice, black tea, and cranberry juice were purified and immobilized onto thiol-activated Sepharose beads. This material was used in pull-down type assays for the capture of LPS. The binding of LPS by PACs has been shown to compete with that of polymyxin B which is known to bind the lipid A component of LPS. Assays conducted in the presence of lipid A verified that at least some component of the LPS binding activity of the PACs is via the lipid A moiety. Molar comparison of polymyxin B to proanthocyanidins indicated that the Sepharose immobilized PACs have a binding affinity for LPS similar to that of polymyxin B.
Johnson
Brandy J
BJ
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, D.C. 20375-5348, USA. brandy.white@nrl.navy.mil
Delehanty
James B
JB
Lin
Baochuan
B
Ligler
Frances S
FS
D001419:Bacteria / Q000737:chemistry*; D005453:Fluorescence; D008070:Lipopolysaccharides / Q000737:chemistry*; D044945:Proanthocyanidins / Q000032:analysis*
D008070:Lipopolysaccharides; D044945:Proanthocyanidins