[Association between cardiac troponin I phosphorylation/degradation and cardiomyopathies].
OBJECTIVE
To investigate the association between cTnI phosphorylation/degradation and cardiomyopathies in extransplanted myocardium.


METHODS
cTnI phosphorylation and degradation as well as PKC (beta1, beta2) expressions were determined in extransplanted hearts from patients with cardiomyopathies (n = 8) and from traffic accidents (n = 6) by Western blot.


RESULTS
The cTnI bands were observed in LV myocardium of cardiomyopathy patients and normal myocardium while and cTnI degradation bands were only detected in LV myocardium from patients with cardiomyopathies. The phosphorylated cTnI bands were significantly upregulated in LV myocardium of cardiomyopathy patients compared to normal myocardium (P < 0.05). There was no myocardial PKCbeta1, PKCbeta2 expression in all examined hearts.


CONCLUSIONS
The cTnI degradation products and increased phosphorylated cTnI expression are likely involved in the pathogenesis and development of cardiomyopathy.
Zhu
Chun-Xia
CX
Institute of Cardiovascular Diseases, Clinical Bio Diagnostic & Bio Therapeutic Laboratory, Department of Cardiology, First Afilliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Wu
Heng-Fang
HF
Chen
Xiang-Jian
XJ
D000328:Adult; D009202:Cardiomyopathies / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D064546:Protein Kinase C beta; D015398:Signal Transduction; D019210:Troponin I / Q000378:metabolism*
D019210:Troponin I; D011493:Protein Kinase C; D064546:Protein Kinase C beta