Pentamidine binds to tRNA through non-specific hydrophobic interactions and inhibits aminoacylation and translation.
The selective and potent inhibition of mitochondrial translation in Saccharomyces cerevisiae by pentamidine suggests a novel antimicrobial action for this drug. Electrophoresis mobility shift assay, T1 ribonuclease footprinting, hydroxyl radical footprinting and isothermal titration calorimetry collectively demonstrated that pentamidine non-specifically binds to two distinct classes of sites on tRNA. The binding was driven by favorable entropy changes indicative of a large hydrophobic interaction, suggesting that the aromatic rings of pentamidine are inserted into the stacked base pairs of tRNA helices. Pentamidine binding disrupts the tRNA secondary structure and masks the anticodon loop in the tertiary structure. Consistently, we showed that pentamidine specifically inhibits tRNA aminoacylation but not the cognate amino acid adenylation. Pentamidine inhibited protein translation in vitro with an EC(50) equivalent to that binds to tRNA and inhibits tRNA aminoacylation in vitro, but drastically higher than that inhibits translation in vivo, supporting the established notion that the antimicrobial activity of pentamidine is largely due to its selective accumulation by the pathogen rather than by the host cell. Therefore, interrupting tRNA aminoacylation by the entropy-driven non-specific binding is an important mechanism of pentamidine in inhibiting protein translation, providing new insights into the development of antimicrobial drugs.
Sun
Tao
T
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China.
Zhang
Yi
Y
D049148:Aminoacylation / Q000187:drug effects*; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology; D000926:Anticodon / Q000737:chemistry; D001483:Base Sequence; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects; D010419:Pentamidine / Q000737:chemistry* / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D012343:RNA, Transfer / Q000737:chemistry / Q000187:drug effects*; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000187:drug effects
D000890:Anti-Infective Agents; D000926:Anticodon; D011500:Protein Synthesis Inhibitors; D012356:RNA, Transfer, Leu; D010419:Pentamidine; D012343:RNA, Transfer