title: Design, synthesis, and amplification of DNA pools for construction of combinatorial pools and libraries.
abstract: This unit describes the design, synthesis, and amplification of random-sequence DNA pools, from which functional nucleic acid-binding or catalytic species can be selected. Since it is an expensive and time-consuming process, the authors have provided an extensive strategic planning section to guide investigators in designing and constructing the pool.
authors.affiliation: Mintz Levin Cohn Glovsky Ferris and Popeo, Boston, Massachusetts, USA.
authors.forename: J
authors.initials: J
authors.lastname: Pollard
authors.forename: S D
authors.initials: SD
authors.lastname: Bell
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004247:DNA / Q000138:chemical synthesis / Q000235:genetics*; D017931:DNA Primers; D015195:Drug Design; D005784:Gene Amplification; D015723:Gene Library; D012313:RNA / Q000235:genetics; D012107:Research Design
chemical_list: D017931:DNA Primers; D012313:RNA; D004247:DNA