Evaluation of different phenol hydroxylase-possessing phenol-degrading pseudomonads by kinetic parameters.
Phenol-degrading pseudomonads possessing different phenol hydroxylases (PH) were evaluated by the values of apparent half-saturation constant for phenol-oxygenating activity (K ( S )), maximum specific growth rate (mu (max)), lag-time length (lambda), inhibition constant (K ( I )) and growth yield factor (Y ( X/S )). Strains of the same PH type showed similar kinetic parameters: single-component PH (sPH) harbouring strains had higher values of K ( S ) and lower values of mu (max) than the strains having multicomponent PH (mPH). However, the values of K ( I ) and the dependencies of the lag-time length on initial phenol concentration were strain-specific. The elevated ratio between specific activities of catechol 1,2-dioxygenase (C12O) and muconate cycloisomerase in sPH-strains caused irreversible accumulation of a high amount of exogenous cis,cis-muconate (CCM) which resulted in decreased Y ( X/S ) values. Co-presence of sPH and mPH genes did not give the strains PC16 and P69 any extra advantage and according to determined kinetic parameters only one PH was active during phenol degradation. At the same time simultaneous functioning of catechol ortho and meta cleavage pathways (strain PC20) resulted in higher mu (max) and Y ( X/S ) values. Evaluation of strains showed that the type of PH determined the efficiency of phenol degradation, whereas the tolerance to elevated phenol concentrations was strain-specific.
Viggor
Signe
S
Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, Tartu, Estonia. signe.viggor@ut.ee
Heinaru
Eeva
E
Künnapas
Allan
A
Heinaru
Ain
A
D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D010636:Phenols; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase