An aquaporin-like protein from the ovaries and gut of American dog tick (Acari: Ixodidae).
A cDNA encoding a putative aquaporin was cloned from the ovaries of the American dog tick, Dermacentor variabilis (Say) (Acari: Ixodidae). The encoded protein is most similar to the vertebrate aquaporin 9 protein family. Localization by reverse transcription-polymerase chain reaction (RT-PCR) shows expression in the gut and ovaries of adult females but not in the synganglion, Malpighian tubules, or salivary glands. Quantitative RT-PCR indicates that it is primarily expressed in the ovaries, with approximately 146 times more transcript than in the gut. When expressed in Xenopus oocytes, the aquaporin-like protein localized to the plasma membrane.
Holmes
Steven P
SP
Naval Facilities Engineering Command, Norfolk, VA 23508, USA. steven.p.holmes@navy.mil
Li
Dimin
D
Ceraul
Shane M
SM
Azad
Abdu F
AF
D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins / Q000032:analysis / Q000145:classification / Q000302:isolation & purification*; D001483:Base Sequence; D005260:Female; D041981:Gastrointestinal Tract / Q000737:chemistry*; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000032:analysis / Q000145:classification / Q000302:isolation & purification*; D026863:Ixodidae / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes; D010053:Ovary / Q000737:chemistry*; D021381:Protein Transport; D014981:Xenopus
D020346:Aquaporins; D019476:Insect Proteins