title: Purification, crystallization and preliminary crystallographic analysis of a 6-pyruvoyltetrahydropterin synthase homologue from Esherichia coli.
abstract: 6-Pyruvoyltetrahydropterin synthase from E. coli (ePTPS) has been crystallized using the hanging-drop vapour-diffusion method. Hexagonal- and rectangular-shaped crystals were obtained. Diffraction data were collected from the hexagonal and rectangular crystals to 3.0 and 2.3 A resolution, respectively. The hexagonal plate-shaped crystals belonged to space group P321, with unit-cell parameters a = b = 112.59, c = 68.82 A , and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I222, with unit-cell parameters a = 112.76, b = 117.66, c = 153.57 A , and contained six molecules in the asymmetric unit. The structure of ePTPS in both crystal forms has been determined by molecular replacement.
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-711, Republic of Korea.
authors.forename: Kyung Hye
authors.initials: KH
authors.lastname: Seo
authors.lastname: Supangat
authors.forename: Hye Lim
authors.initials: HL
authors.lastname: Kim
authors.forename: Young Shik
authors.initials: YS
authors.lastname: Park
authors.forename: Che  Ok
authors.initials: C
authors.lastname: Jeon
authors.forename: Kon Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D019761:Phosphorus-Oxygen Lyases; C043497:6-pyruvoyltetrahydropterin synthase