Recurrent pregnancy loss and frequency of eight antiphospholipid antibodies and genetic thrombophilic factors in Czech women.
OBJECTIVE
The aim of this study was to investigate frequencies of eight antiphospholipid antibodies (aPLs) in serum, four genetic thrombophilic factors and their mutual relation in 206 patients with repeated pregnancy loss (RPL).


METHODS
Enzyme-linked immunosorbent assay was used for detection of aPLs against ph-serine, ph-ethanolamine, ph-inositol, DL-glycerol, phosphatidic acid, anti-annexin V, cardiolipin, and beta2-GPI. FV 1691G>A (Leiden mutation), FII 20210G>A mutation, MTHFR 677C>T and MTHFR 1298A>C variant genotypes were determined using a melting curve analysis of the PCR amplification product detected by the fluorescence resonance energy transfer. Genotypic distribution and allelic frequencies were calculated. Correlation between aPLs and thrombophilic factors was tested by chi-square and Fisher exact test.


RESULTS
Our results show significantly increased prevalence of aPLs against ph-inositol (17-19.6% dependent on number of spontaneous miscarriages) and against ph-serine (18-25%). aPLs in IgG prevail. In 96% of the studied group, at least one risk factor was found (either aPLs positivity or thrombophilic factor). Both aPLs and thrombophilic factors were present in 43%. In the group of women with three or more RPLs, strong positive correlation of aPLs positivity and thrombophilic risk factors was observed.


CONCLUSIONS
Antiphospholipide antibodies and genetic thrombophilic factors are important risk factors in the pathogenesis of RPL. Both autoantibodies against various kinds of phospholipides and genetic thrombophilic factors must be studied together in diagnosis of RPL for appropriate treatment.
Subrt
Ivan
I
Institute of Medical Genetics, Department of Obstetrics and Gynecology, Charles University and Faculty Hospital, Pilsen, Czech Republic. subrt@fnplzen.cz
Ulcova-Gallova
Zdenka
Z
Bibkova
Katarina
K
Micanova
Zdenka
Z
Hejnalova
Marketa
M
Cerna
Monika
M
Hradecky
Libor
L
Novotny
Zdenek
Z
D000026:Abortion, Habitual / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000096:biosynthesis / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D015984:Causality; D003430:Cross-Sectional Studies; D018153:Czech Republic; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency / Q000276:immunology; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D011247:Pregnancy; D011256:Pregnancy Outcome; D012307:Risk Factors; D019851:Thrombophilia / Q000235:genetics* / Q000276:immunology*
D017152:Antibodies, Antiphospholipid; D015342:DNA Probes