Molecular identification of Trypanosoma cruzi I tropism for central nervous system in Chagas reactivation due to AIDS.
Trypanosoma cruzi lineages, microsatellite allelic polymorphism, and mithocondrial gene haplotypes were directly typified from peripheral blood and cerebrospinal fluid specimens of a Bolivian patient with Chagas disease with accompanying AIDS and central nervous system severe involvement. Of note, the patient's blood was infected by a mixture of T. cruzi I and T. cruzi IId/e polyclonal populations while the cerebrospinal fluid showed only a monoclonal T. cruzi I population. Our findings do not corroborate the original assumption of innocuity for T. cruzi I in the southern cone of the Americas and highlight lineage I tropism for central nervous system causing lethal Chagas reactivation.
Burgos
Juan M
JM
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina.
Begher
Sandra
S
Silva
Helder M Valadares
HM
Bisio
Margarita
M
Duffy
Tomas
T
Levin
Mariano J
MJ
Macedo
Andrea M
AM
Schijman
Alejandro G
AG
D017088:AIDS-Related Opportunistic Infections / Q000469:parasitology*; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D000818:Animals; D001838:Bolivia; D002490:Central Nervous System / Q000469:parasitology; D020808:Central Nervous System Protozoal Infections / Q000175:diagnosis / Q000209:etiology / Q000469:parasitology*; D014355:Chagas Disease / Q000209:etiology* / Q000469:parasitology; D016054:DNA, Protozoan / Q000097:blood / Q000134:cerebrospinal fluid; D003576:Electron Transport Complex IV / Q000235:genetics; D017809:Fatal Outcome; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012008:Recurrence; D018523:Tropism / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
D016054:DNA, Protozoan; D003576:Electron Transport Complex IV