Array-CGH reveals hidden gene dose changes in children with acute lymphoblastic leukaemia and a normal or failed karyotype by G-banding.
A tiling path 33K BAC array was used to study 28 children with acute lymphoblastic leukaemia (ALL) who had normal or failed G-banded karyotypes. Twenty-two patients (79%) had a total of 135 copy number alterations (CNA) (69 gains and 66 losses); most of these patients showed CNA that were below the resolution of G-banding. Molecular cytogenetic and array comparative genomic hybridization results enabled the division of B-precursor ALL patients into five groups: high hyperdiploidy, intrachromosomal amplification of 21q, ETV6/RUNX1 rearrangement, others and no CNA. Apart from a shared deletion of 9p21.3, T-ALL patients had additional small CNA, with no region in common.
Kuchinskaya
Ekaterina
E
Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden. Ekaterina.Kuchinskaya@ki.se
Heyman
Mats
M
Nordgren
Ann
A
Schoumans
Jacqueline
J
Staaf
Johan
J
Borg
Ake
A
Söderhäll
Stefan
S
Grandér
Dan
D
Nordenskjöld
Magnus
M
Blennow
Elisabeth
E
D002648:Child; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*