Novel roles for murine complement receptors type 1 and 2 II. Expression and function of CR1/2 on murine mesenteric lymph node T cells.
In mice CR1 and CR2 are encoded at a single locus and the two alternatively spliced RNA transcripts of the CD21 gene generate murine CR1 and CR2 (CR1/2). While the function of CD21 has been extensively studied on murine B lymphocytes, much less is known about its appearance and role on T cells. Earlier we had demonstrated the expression of CR1/2 on activated murine T cells by cytofluorymetry and immunoprecipitation and proposed its role in the enhancement of antigen presentation by B cells and macrophages bearing C3-fragments. In this study we analyze the expression-profile and further possible roles of CR1/2 on T lymphocytes. We describe a CR1/2 expressing CD4+ T cell subpopulation present in the mesenteric lymph nodes of mice. We show that these cells can be activated by the CD21-specific 7G6 single chain antibody, and demonstrate that activation via this complement receptor results in the translocation of NF-kappaB to the nucleus. Interestingly we found a substantial elevation of CD21+ T cells during both spontaneous and ceramide-induced apoptosis.
Molnár
Eszter
E
Department of Immunology, Loránd Eötvös University, Pázmány Péter s. 1/C, 1117 Budapest, Hungary.
Prechl
József
J
Erdei
Anna
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation; D015870:Gene Expression / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D008643:Mesentery / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D021381:Protein Transport / Q000276:immunology; D017463:Receptors, Complement 3b / Q000276:immunology*; D017464:Receptors, Complement 3d / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016328:NF-kappa B; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; D002118:Calcium