Enhanced subtyping scheme for Salmonella enteritidis.
To improve pulsed-field gel electrophoresis-based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations. One 3-enzyme subset, SfiI/PacI/NotI, was highly discriminatory. Five different indices, including the Simpson diversity index, supported this 3-enzyme combination for improved differentiation of S. Enteritidis.
Zheng
Jie
J
University of Maryland, College Park, Maryland, USA.
Keys
Christine E
CE
Zhao
Shaohua
S
Meng
Jianghong
J
Brown
Eric W
EW
D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D010802:Phylogeny; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics