Changes of high mobility group box 1 in serum of pig acute hepatic failure model and significance.
The role of the high mobility group box 1 (HMGB-1) in acute hepatic failure and the effect of artificial liver support system treatment on HMGB-1 level were investigated. Pig models of acute hepatic failure were induced by D-galactosamine and randomly divided into two groups with or without artificial liver support system treatment. Tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) levels were detected by the enzyme linked immunosorbent assay (ELISA), the expression of HMGB-1 by Western blot, and serum levels of HMGB-1, liver function and hepatic pathology were observed after artificial liver support system treatment. The levels of TNF-alpha and IL-1 beta were increased and reached the peak at 24th h in the acute hepatic failure group, then quickly decreased. The serum level of HMGB-1 was increased at 24th h in the acute hepatic failure group and reached the peak at 48th h, then kept a stable high level. Significant liver injury appeared at 24th h and was continuously getting worse in the pig models of acute hepatic failure. In contrast, the liver injury was significantly alleviated and serum level of HMGB-1 was significantly decreased in the group treated with artificial liver support system (P<0.05). It was suggested that HMGB-1 may participate in the inflammatory response and liver injury in the late stage of the acute liver failure. Artificial liver support system treatment can reduce serum HMGB-1 level and relieve liver pathological damage.
Zhang
Fan
F
Department of Infectious Disease, Union Hospital, Tongji Medical Colleage, Huazhong University of Science and Technology, Wuhan 430022, China. zf77361@yahoo.com.cn
He
Yongwen
Y
Duan
Zhongping
Z
D000818:Animals; D002779:Cholestasis; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D024243:HMGB1 Protein / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D053583:Interleukin-1beta / Q000097:blood; D008099:Liver / Q000378:metabolism*; D017093:Liver Failure / Q000097:blood* / Q000378:metabolism*; D019164:Liver, Artificial; D009336:Necrosis; D013552:Swine; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D024243:HMGB1 Protein; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha