[Extraction and content determination of polysaccharides in Viscum coloratum].
OBJECTIVE
To establish a method for extraction and content determination of polysaccharide in Viscum coloratum.


METHODS
Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content determination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent.


RESULTS
The content of polyaccharide in V. coloratum, CVPS-III, and CVPS-III-C were respectively 4.93% (RSD 1.04%, n = 3), 43.28% (RSD 1.39%, n =3), 69.55% (RSD 1.62%, n = 3), and the average recovery was 96.07% (RSD 2.54%, n = 5).


CONCLUSIONS
The method was simple, rapid, and accurate.
Wang
Jun
J
College of Biology and Environmental Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018, China. wangjunjs@tom.com
Zhu
Yi-fan
YF
D002852:Chromatography, Ion Exchange / Q000379:methods; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D028181:Viscum / Q000737:chemistry*
D011134:Polysaccharides