title: [Serology as a diagnostic tool for surveillance and control of schistosomiasis in Holambra, São Paulo State, Brazil].
abstract: The present study aimed to evaluate the incorporation of the immunofluorescence test (IFT) with adult parasite paraffin sections as antigen substrate for the detection of IgM antibodies (IgM-IFT), as a diagnostic method in the schistosomiasis control program in the county (municipality) of Holambra, São Paulo State, Brazil. This city was selected for this study based on its low endemicity for schistosomiasis, the first cases having been reported in 1993, and because of the need to implement a control program with more sensitive diagnostic techniques. 202 individuals underwent IgM-IFT, with 48 serologically positive cases; of these, 28 were tested with the Kato-Katz technique, using three stool samples. Schistosoma mansoni eggs were found in 14 individuals, with egg counts varying from 2.7 to 224 per gram of stool. The results indicate the potential usefulness of IgM-IFT as a screening test, subject to subsequent confirmation using a parasitological method, in low-endemic areas for schistosomiasis.
authors.affiliation: Instituto Adolfo Lutz, São Paulo, Brasil. cgargion@ial.sp.gov.br
authors.forename: Cybele
authors.initials: C
authors.lastname: Gargioni
authors.forename: Rita Maria da
authors.initials: RM
authors.lastname: Silva
authors.forename: Célia Maria
authors.initials: CM
authors.lastname: Thomé
authors.forename: Celma Maria da Silva
authors.initials: CM
authors.lastname: Quadros
authors.forename: Hermínia Yohko
authors.initials: HY
authors.lastname: Kanamura
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D001938:Brazil / Q000453:epidemiology; D002648:Child; D005243:Feces / Q000469:parasitology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control; D012698:Serologic Tests
chemical_list: D000909:Antibodies, Helminth; D007075:Immunoglobulin M