title: Dehydrothermal treatment of collagen influences on bone regeneration by octacalcium phosphate (OCP) collagen composites.
abstract: We have engineered a scaffold constructed of synthetic octacalcium phosphate (OCP) and collagen composites (OCP-collagen) and report that OCP-collagen significantly enhanced bone regeneration more than the implantation of OCP. We hypothesized that the dehydrothermal treatment (DHT) during the fabrication of OCP-collagen might influence bone regeneration by OCP-collagen. To examine this hypothesis, bone regeneration by the implantation of OCP-collagen with DHT [OCP/Col(+)] was compared with that by OCP-collagen without DHT [OCP/Col(-)]. It was confirmed that both OCP/Col(+) and OCP/Col(-) contained the characteristics of OCP structure in X-ray diffraction. Before implantation, calcium deposition derived from OCP was observed within the collagen of both OCP/Col(+) and OCP/Col(-) by undecalcified histological sections. OCP/Col(+) or OCP/Col(-) was implanted into the critical-sized defects in rat crania. Radiographic and histological examination was performed and the percentage of newly formed bone (n-Bone%) in the defect was determined by a histomorphometrical analysis. N-Bone% treated with OCP/Col(+) was significantly higher than that with OCP/Col(-) at 4 and 12 weeks after implantation, because fast degradation of the implanted collagen of OCP/Col(-) elicited disappearance of the scaffold for bone regeneration. The stiffness of the calcified collagen in OCP-collagen would be more important than the existence of calcified collagen to enhance the bone regeneration by OCP-collagen composites. The present study suggests that the dehydrothermal treatment would influence effective bone regeneration by OCP-collagen.
authors.affiliation: Division of Clinical Cell Therapy, Department of Translational Research, Center for Translational and Advanced Animal Research (CTAAR), Tohoku University School of Medicine, Aoba-Ku, Sendai 980-8574, Japan. kamakura@mail.tains.tohoku.ac.jp
authors.forename: Shinji
authors.initials: S
authors.lastname: Kamakura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sasaki
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Honda
authors.forename: Takahisa
authors.initials: T
authors.lastname: Anada
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsui
authors.forename: Seishi
authors.initials: S
authors.lastname: Echigo
authors.forename: Osamu
authors.initials: O
authors.lastname: Suzuki
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; C022045:octacalcium phosphate; D003094:Collagen; D002118:Calcium