Cytokine profiling using monocytes/macrophages cultured on common biomaterials with a range of surface chemistries.
Cytokines, chemokines, and growth factors were assayed from the supernatants of monocytes and macrophages cultured on common biomaterials with a range of surface chemistries. TNF-alpha, MCP-1, MIP-1alpha, IL-8, IL-6, IL-1beta, VEGF, IL-1ra, and IL-10 were measured from monocyte/macrophage cultures at different stages of activation and differentiation seeded onto polyethylene, polyurethane, expanded polytetrafluoroethylene, polymethyl methacrylate, and a hydrogel copolymer of 2-hydroxyethyl methacrylate, 1-vinyl-2-pyrrolidinone, and polyethylene glycol acrylate in tissue culture polystyrene (TCPS) plates. Empty TCPS wells and organo-tin polyvinyl chloride served as "blanks" and positive controls, respectively. Results showed an overall increase in cytokine, chemokine, and growth factor production as monocytes are activated or differentiated into macrophages and that proinflammatory and anti-wound healing cytokines and chemokines dominate this profile. However, cytokine production was only modestly affected by the surface chemistry of these four stable and noncytotoxic biomaterials.
Schutte
Robert J
RJ
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
Parisi-Amon
Andreina
A
Reichert
W Monty
WM
D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D018925:Chemokines / Q000032:analysis / Q000378:metabolism; D016207:Cytokines / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D008722:Methods; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D011108:Polymers; D013499:Surface Properties
D001672:Biocompatible Materials; D018925:Chemokines; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D011108:Polymers