title: Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient.
abstract: In order to investigate the potential of a Sanguisorba officinalis root extract as an active ingredient for wrinkle-care cosmetics, we measured its free radical scavenging activity, elastase inhibitory activity, expression of MMP-1 (matrix metalloprotease-1) in vitro, and type I collagen synthesis in normal human fibroblast cells. To isolate the main components from the S. officinalis root extract, we purified the extract by solvent fractionation, column chromatography, and recrystallization. The active component was identified as ziyuglycoside I by a spectroscopic analysis. Ziyuglycoside I increased the expression of type I collagen in a dose-dependent manner (by up to 71.3% at 50 muM). A clinical study of a formulation containing ziyuglycoside I, which involved visual evaluation and image analysis, showed a significantly different effect (p<0.05) of the test formulation from that of the placebo. This result suggests that ziyuglycoside I isolated from S. officinalis root extract could be used as an active ingredient for cosmetics.
authors.affiliation: R&D Center, Bioland Ltd., Songjeong, Byongchon, Cheonan, Chungnam 330-863, Republic of Korea.
authors.forename: Young Heui
authors.initials: YH
authors.lastname: Kim
authors.forename: Chan Bok
authors.initials: CB
authors.lastname: Chung
authors.forename: Jin Guk
authors.initials: JG
authors.lastname: Kim
authors.forename: Kang Il
authors.initials: KI
authors.lastname: Ko
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Jong-Heon
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Yong
authors.initials: SY
authors.lastname: Eom
authors.forename: Young Sil
authors.initials: YS
authors.lastname: Kim
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Hwang
authors.forename: Ki Ho
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D003358:Cosmetics; D003460:Crystallization; D017931:DNA Primers; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D008875:Middle Aged; D010919:Placebos; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D024042:Collagen Type I; D003358:Cosmetics; D017931:DNA Primers; D016166:Free Radical Scavengers; D010919:Placebos; D010936:Plant Extracts; D012503:Saponins; C484848:ziyuglycoside I; D020781:Matrix Metalloproteinase 1