title: RNAi in transgenic plants.
abstract: RNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus-vectors. This unit provides Basic Protocols for transgene and virus induced silencing. It also includes a Basic Protocol for protein overexpression using viral proteins that suppress silencing.
authors.affiliation: Iowa State University, Ames, Iowa, USA.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Yin
authors.forename: Joanne
authors.initials: J
authors.lastname: Chory
authors.forename: David
authors.initials: D
authors.lastname: Baulcombe
mesh_terms: D035843:Biomedical Research / Q000379:methods*; D020868:Gene Silencing; D007202:Indicators and Reagents; D030821:Plants, Genetically Modified / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D007202:Indicators and Reagents; D012330:RNA, Double-Stranded; D018749:RNA, Plant; D034741:RNA, Small Interfering