title: Preparation of myeloma cells.
abstract: In this unit, myeloma cells are cultured to ensure their sensitivity to the HAT selection medium used after cell fusion. Cell culture conditions are adjusted such that the Sp2/0 cells are in the log phase of growth and exhibit high viability at the time of collection for fusion. A support protocol is provided to determine the number of viable cells present in the cell culture.
authors.affiliation: Allelix Inc., Mississauga, Ontario.
authors.forename: S A
authors.initials: SA
authors.lastname: Fuller
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: J G
authors.initials: JG
authors.lastname: Hurrell
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification*; D018929:Cell Culture Techniques; D042381:Granulocyte Precursor Cells / Q000276:immunology*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal