title: Simultaneous determination of adenosine triphosphate and its metabolites in human whole blood by RP-HPLC and UV-detection.
abstract: To obtain insight in mechanisms of action of extracellular adenosine triphosphate (ATP) and adenosine, a simple HPLC method has been optimized and applied to investigate ATP metabolism in human whole blood ex vivo. This method provided good chromatographic resolution and peak shape for all eight compounds within a 19 min run time. The baseline was clean, the lower limit of quantification was below 0.3 micromol/L for all adenine nucleotides and the method demonstrated good linearity. Within-day precision ranged from 0.7 to 5.9% and between-days from 2.6 to 15.3%. Simplicity and simultaneous detection of ATP and its metabolites make this method suitable for clinical pharmacokinetic studies.
authors.affiliation: Department of Epidemiology, NUTRIM, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. erik.coolen@epid.unimaas.nl <erik.coolen@epid.unimaas.nl>
authors.forename: Erik J C M
authors.initials: EJ
authors.lastname: Coolen
authors.forename: Ilja C W
authors.initials: IC
authors.lastname: Arts
authors.forename: Els L R
authors.initials: EL
authors.lastname: Swennen
authors.forename: Aalt
authors.initials: A
authors.lastname: Bast
authors.forename: Martien A Cohen
authors.initials: MA
authors.lastname: Stuart
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Dagnelie
mesh_terms: D000227:Adenine Nucleotides / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate