Tissue collection for systematic phenotyping in the mouse.
In this unit, a procedure for post-mortem examination of mice and tissue collection is provided. This procedure is performed for post-mortem analysis of anatomical defects (necropsy) and histological analysis and/or tissue collection destined for molecular biology applications. In both cases, tissue preservation is the major issue, but the way to achieve it depends on the objective. When histological analysis is the aim, tissue preservation is achieved by rapid transfer into fixative solutions. In contrast, molecular biology applications require rapid freezing of tissue samples to preserve mRNA integrity. Consequently, performing both procedures simultaneously may be at the expense of the final product quality.
Antal
Cristina
C
Institut Clinique de la Souris, Illkirch Cedex, France.
Teletin
Marius
M
Wendling
Olivia
O
Dgheem
Mounzer
M
Auwerx
Johan
J
Mark
Manuel
M
D000818:Animals; D005260:Female; D005404:Fixatives / Q000737:chemistry; D005615:Freezing; D008297:Male; D051379:Mice; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016707:Tissue Fixation / Q000379:methods; D014021:Tissue Preservation / Q000379:methods*; D020858:Tissue and Organ Harvesting / Q000379:methods
D005404:Fixatives; D012333:RNA, Messenger