An alloantibody to a high-prevalence MNS antigen in a person with a GP.JL/Mk phenotype.
The low-prevalence MNS blood group antigenTSEN is located at the junction of glycophorin A (GPA) to glycophorin B (GPB) in several hybrid glycophorin molecules. Extremely rare people have RBCs with a double dose of the TSEN antigen and have made an antibody to a high-prevalence MNS antigen. We report the first patient who is heterozygous for GYP.JL and Mk. During prenatal tests,an alloantibody to a high-prevalence antigen was detected in the serum of a 21-year-old Hispanic woman. The antibody detected an antigen resistant to treatment by papain, trypsin, alpha-chymotrypsin, or DTT. The antibody was strongly reactive by the IAT with all RBCs tested except those having the MkMk, GP.Hil/GP.Hil, or GP.JL/GP.JL phenotypes. The patient's RBCs typed M+N-S+/-s-U+, En(a+/-), Hut-, Mi(a-), Mur-, Vw-, Wr(a-b-), and were TSEN+, MINY+. Reactivity with Glycine soja suggested that her RBCs had a decreased level of sialic acid. Immunoblotting showed the presence of monomer and dimer forms of a GP(A-B) hybrid and an absence of GPA and GPB. Sequencing of DNA and PCR-RFLP using the restriction enzyme RsaI confirmed the presence of a hybrid GYP(AB). The patient's antibody was determined to be anti-EnaFR. She is the first person reported with the GP.JL phenotype associated with a deletion of GYPA and GYPB in trans to GYP.JL.
Ratliff
J
J
BloodSource, Sacramento, CA, USA.
Veneman
S
S
Ward
J
J
Lomas-Francis
C
C
Hue-Roye
K
K
Velliquette
R W
RW
Sausais
L
L
Maldonado
T
T
Miyamoto
J
J
Martin
Y
Y
Slater
D
D
Reid
M E
ME
D000328:Adult; D001483:Base Sequence; D001788:Blood Grouping and Crossmatching / Q000379:methods; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000276:immunology; D004912:Erythrocytes / Q000737:chemistry / Q000276:immunology; D005260:Female; D005838:Genotype; D006021:Glycophorins / Q000737:chemistry* / Q000276:immunology*; D006384:Hemagglutination / Q000276:immunology; D006801:Humans; D007231:Infant, Newborn; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D007519:Isoantigens / Q000737:chemistry*; D008951:MNSs Blood-Group System / Q000235:genetics*; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length / Q000276:immunology; D011247:Pregnancy; D013030:Spain
C409973:GYPB protein, human; D006021:Glycophorins; D007518:Isoantibodies; D007519:Isoantigens; D008951:MNSs Blood-Group System