Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study.
It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in biopsies of dermis from young and old human donors. TGF-beta1 was up-regulated in the dermis of old donors as well as the TGF-beta1-regulated genes. The anti-oxidant enzymes Selenium-dependent Glutathione peroxidase and Glutatione S-Transferase Theta 1 were also up-regulated in old dermis as well as Tumor Necrosis Factor Receptor Superfamily 1A. None of these genes had altered expression level in skin fibroblasts embedded in a collagen matrix and exposed to sublethal doses of UVB, suggesting their involvement in intrinsic aging. This study represents a proof-of-concept of larger whole transcriptome studies where all avenues should be used to subtract changes in gene expression due to extrinsic aging from changes potentially due to intrinsic aging.
Borlon
Céline
C
Research Unit on Cellular Biology (URBC), Department of Biology, Faculty of Sciences, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium.
Weemaels
Geoffroy
G
Godard
Patrice
P
Debacq-Chainiaux
Florence
F
Lemaire
Pierre
P
Deroanne
Christophe
C
Toussaint
Olivier
O
D000375:Aging / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D017931:DNA Primers; D012333:RNA, Messenger; D005982:Glutathione Transferase