title: The genome landscape of ERalpha- and ERbeta-binding DNA regions.
abstract: In this article, we have applied the ChIP-on-chip approach to pursue a large scale identification of ERalpha- and ERbeta-binding DNA regions in intact chromatin. We show that there is a high degree of overlap between the regions identified as bound by ERalpha and ERbeta, respectively, but there are also regions that are bound by ERalpha only in the presence of ERbeta, as well as regions that are selectively bound by either receptor. Analysis of bound regions shows that regions bound by ERalpha have distinct properties in terms of genome landscape, sequence features, and conservation compared with regions that are bound by ERbeta. ERbeta-bound regions are, as a group, located more closely to transcription start sites. ERalpha- and ERbeta-bound regions differ in sequence properties, with ERalpha-bound regions having an overrepresentation of TA-rich motifs including forkhead binding sites and ERbeta-bound regions having a predominance of classical estrogen response elements (EREs) and GC-rich motifs. Differences in the properties of ER bound regions might explain some of the differences in gene expression programs and physiological effects shown by the respective estrogen receptors.
authors.affiliation: Department of Biosciences and Nutrition, Karolinska Institutet, Novum, SE-14157 Huddinge, Sweden.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Troels Torben
authors.initials: TT
authors.lastname: Marstrand
authors.forename: Anders
authors.initials: A
authors.lastname: Ström
authors.forename: Eivind
authors.initials: E
authors.lastname: Valen
authors.forename: Albin
authors.initials: A
authors.lastname: Sandelin
authors.forename: Jan-Ake
authors.initials: JA
authors.lastname: Gustafsson
authors.forename: Karin
authors.initials: K
authors.lastname: Dahlman-Wright
mesh_terms: D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000276:immunology / Q000378:metabolism*; D016678:Genome / Q000235:genetics*; D006801:Humans; D011485:Protein Binding
chemical_list: D000906:Antibodies; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004247:DNA