title: Preparing lambda DNA from phage lysates.
abstract: DNA extracted from lambda-derived vectors is typically subcloned into plasmid or filamentous phage vectors. The first two protocols describe methods for isolating phage DNA from large- and medium-scale liquid lysates. These two methods use either density-gradient centrifugation or ion-exchange chromatography to purify the phage particles. The third protocol describes a rapid procedure for isolating phage DNA, suitable for small-scale liquid lysates.
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Lech
authors.forename: K J
authors.initials: KJ
authors.lastname: Reddy
authors.forename: L A
authors.initials: LA
authors.lastname: Sherman
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004279:DNA, Viral / Q000302:isolation & purification*; D005822:Genetic Vectors / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*
chemical_list: D004279:DNA, Viral