Antimicrobial properties of avian eggshell-specific C-type lectin-like proteins.
C-type lectin-like proteins are major components of the calcified eggshell of multiple avian species. In this study, two representative avian C-type lectin-like proteins, ovocleidin-17 and ansocalcin, were purified from decalcified chicken and goose eggshell protein extracts and investigated for carbohydrate binding activity as well as antimicrobial activity. Purified ovocleidin-17 and ansocalcin were found to bind bacterial polysaccharides, and were bactericidal against Bacillus subtilis, Staphylococcus aureus and Pseudomona aeruginosa. Bactericidal activity was found to be enhanced in the presence of calcium but was not dependent on its presence. The results suggest that avian C-type lectin-like proteins may play an important antimicrobial role in defence of the avian embryo.
Wellman-Labadie
Olivier
O
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Lakshminarayanan
Rajamani
R
Hincke
Maxwell T
MT
D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology; D030161:Avian Proteins / Q000378:metabolism; D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D045424:Complex Mixtures; D004527:Egg Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D004528:Egg Shell / Q000187:drug effects / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005777:Geese / Q000378:metabolism*; D037181:Lectins, C-Type / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008837:Micrococcus / Q000166:cytology / Q000187:drug effects; D008958:Models, Molecular
D000890:Anti-Infective Agents; D030161:Avian Proteins; D045424:Complex Mixtures; D004527:Egg Proteins; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; C095273:ovocleidin 17