Atbf1 is required for the Pit1 gene early activation.
Enhancers have been functionally described for >35 years, but the molecular principles underlying the integration of regulatory inputs to alternate gene enhancers used during mammalian organogenesis remain incompletely understood. Using a combination of in vivo enhancer mapping and proteomics approaches, we have established that two distant and distinct early enhancers, each requiring different transcription complexes, are required for full activation of the gene encoding the pituitary lineage determining factor, Pit1. A transcription factor belonging to the "giant, multiple-homeodomain and zinc finger family," Atbf1, serves as a novel pituitary regulator for one of the two required enhancers as shown by genetic and in vitro analysis.
Qi
Yingchuan
Y
Howard Hughes Medical Institute and Department of Medicine, School of Medicine, University of California at San Diego, La Jolla, CA 92093, USA.
Ranish
Jeffrey A
JA
Zhu
Xiaoyan
X
Krones
Anna
A
Zhang
Jie
J
Aebersold
Ruedi
R
Rose
David W
DW
Rosenfeld
Michael G
MG
Carrière
Catherine
C
D000818:Animals; D001483:Base Sequence; D019070:Cell Lineage; D053595:Embryonic Stem Cells / Q000378:metabolism; D004843:Epistasis, Genetic; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D016678:Genome / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010902:Pituitary Gland / Q000378:metabolism; D011485:Protein Binding; D040901:Proteomics; D013997:Time Factors; D050817:Transcription Factor Pit-1 / Q000235:genetics / Q000378:metabolism*
D018398:Homeodomain Proteins; D050817:Transcription Factor Pit-1; C489511:Zfhx3 protein, mouse