Diffusion coefficient of fluorescent phosphatidylinositol 4,5-bisphosphate in the plasma membrane of cells.
Phosphatidylinositol 4,5-bisphosphate (PIP(2)) controls a surprisingly large number of processes in cells. Thus, many investigators have suggested that there might be different pools of PIP(2) on the inner leaflet of the plasma membrane. If a significant fraction of PIP(2) is bound electrostatically to unstructured clusters of basic residues on membrane proteins, the PIP(2) diffusion constant, D, should be reduced. We microinjected micelles of Bodipy TMR-PIP(2) into cells, and we measured D on the inner leaflet of fibroblasts and epithelial cells by using fluorescence correlation spectroscopy. The average +/- SD value from all cell types was D = 0.8 +/- 0.2 microm(2)/s (n = 218; 25 degrees C). This is threefold lower than the D in blebs formed on Rat1 cells, D = 2.5 +/- 0.8 microm(2)/s (n = 26). It is also significantly lower than the D in the outer leaflet or in giant unilamellar vesicles and the diffusion coefficient for other lipids on the inner leaflet of these cell membranes. The simplest interpretation is that approximately two thirds of the PIP(2) on inner leaflet of these plasma membranes is bound reversibly.
Golebiewska
Urszula
U
Department of Physiology and Biophysics, Health Science Center, Stony Brook University, Stony Brook, NY 11794-8661, USA.
Nyako
Marian
M
Woturski
William
W
Zaitseva
Irina
I
McLaughlin
Stuart
S
D000818:Animals; D001896:Boron Compounds; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D004847:Epithelial Cells / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D008567:Membranes, Artificial; D018613:Microscopy, Confocal; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C / Q000378:metabolism; D051381:Rats; D012235:Rhodamines; D013050:Spectrometry, Fluorescence
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D008567:Membranes, Artificial; D019269:Phosphatidylinositol 4,5-Diphosphate; D012235:Rhodamines; D054801:Phosphoinositide Phospholipase C