title: Conjugation of enzymes to antibodies.
abstract: Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor the active site of the enzyme is functionally altered. This unit describes procedures for cross-linking HRPO, urease or alkaline phosphatase to immunoaffinity-purified monoclonal or polyclonal antibodies (IgG).
authors.affiliation: ADI Diagnostics, Rexdale, Ontario.
authors.forename: S E
authors.initials: SE
authors.lastname: Winston
authors.forename: S A
authors.initials: SA
authors.lastname: Fuller
authors.forename: M J
authors.initials: MJ
authors.lastname: Evelegh
authors.forename: J G
authors.initials: JG
authors.lastname: Hurrell
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000378:metabolism*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D007124:Immunoenzyme Techniques / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000906:Antibodies; D004798:Enzymes