Preparing lambda DNA from phage lysates.
DNA extracted from lambda-derived vectors is typically subcloned into plasmid or filamentous phage vectors. The first two protocols describe methods for isolating phage DNA from large- and medium-scale liquid lysates. These two methods use either density-gradient centrifugation or ion-exchange chromatography to purify the phage particles. The third protocol describes a rapid procedure for isolating phage DNA, suitable for small-scale liquid lysates.
Lech
K
K
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Reddy
K J
KJ
Sherman
L A
LA
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004279:DNA, Viral / Q000302:isolation & purification*; D005822:Genetic Vectors / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*
D004279:DNA, Viral