Electrospray mass spectrometry to study drug-nucleic acids interactions.
We present here a tutorial review on the electrospray mass spectrometry technique and its applications to the study of drug-nucleic acid non-covalent complexes. Particular emphasis has been made on the basic principles of the technique, to allow even the non-specialist to design fit-for-purpose mass spectrometry experiments and interpret the results. Standard applications will be described in detail, including the determination of stoichiometries and equilibrium binding constants of non-covalent complexes, the study of binding kinetics, and the development of ligand screening assays. We also outline the potentials of more advanced and/or more recent MS-based techniques (tandem mass spectrometry, ion mobility spectrometry and gas-phase spectroscopy) for the study of the nucleic acid-ligand complexes.
Rosu
Frédéric
F
Department of Chemistry, GIGA-R, Sart-Tilman Campus B6c, University of Liège, B-4000 Liège, Belgium. f.rosu@ulg.ac.be
De Pauw
Edwin
E
Gabelica
Valérie
V
D008024:Ligands; D046911:Macromolecular Substances / Q000737:chemistry; D009696:Nucleic Acids / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
D008024:Ligands; D046911:Macromolecular Substances; D009696:Nucleic Acids; D004364:Pharmaceutical Preparations