Whole-mount histochemical detection of beta-galactosidase activity.
This unit describes fixation and staining for b-galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants. To help achieve consistent results with larger samples, an alternate protocol details preparation of thick sections. Support protocols are provided for clearing of tissues for better resolution and paraffin-embedding the whole embryo.
Pereira
F A
FA
Baylor College of Medicine, Houston, Texas, USA.
D000818:Animals; D004622:Embryo, Mammalian; D016591:Histocytological Preparation Techniques; D006801:Humans; D014021:Tissue Preservation; D001616:beta-Galactosidase / Q000032:analysis* / Q000378:metabolism*
D001616:beta-Galactosidase