title: Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis.
abstract: During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient spermatocytes exhibit a failure in chromosomal synapsis. In mutant spermatocytes, DNA double-strand breaks (DSBs) are formed, but localization of the recombination proteins RAD51 and DMC1 to meiotic chromosomes is severely impaired. Based on these data, we propose that TEX15 regulates the loading of DNA repair proteins onto sites of DSBs and, thus, its absence causes a failure in meiotic recombination.
authors.affiliation: Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Sigrid
authors.initials: S
authors.lastname: Eckardt
authors.forename: N Adrian
authors.initials: NA
authors.lastname: Leu
authors.forename: K John
authors.initials: KJ
authors.lastname: McLaughlin
authors.forename: Peijing Jeremy
authors.initials: PJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D019313:BRCA1 Protein / Q000235:genetics; D024682:BRCA2 Protein / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D023902:Chromosome Pairing / Q000235:genetics*; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D004950:Esterases / Q000235:genetics; D005260:Female; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D028044:Phosphate-Binding Proteins; D051135:Rad51 Recombinase / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics; D012727:Sex Characteristics; D013087:Spermatids / Q000378:metabolism* / Q000648:ultrastructure; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D019313:BRCA1 Protein; D024682:BRCA2 Protein; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D004706:Endodeoxyribonucleases; D004950:Esterases; D009687:Nuclear Proteins; D028044:Phosphate-Binding Proteins; D051135:Rad51 Recombinase; D000099257:Meiotic Recombination Protein SPO11; C513032:Dmc1 protein, mouse; C527758:Tex15 protein, mouse