Reduced white fat mass in adult mice bearing a truncated Patched 1.
Hedgehog (Hh) signaling emerges as a potential pathway contributing to fat formation during postnatal development. In this report, we found that Patched 1 (Ptc1), a negative regulator of Hh signaling, was expressed in the epididymal fat pad of adult mice. Reduced total white fat mass and epididymal adipocyte cell size were observed in naturally occurring spontaneous mesenchymal dysplasia (mes) adult mice (Ptc1(mes/mes)), which carry a deletion of Ptc1 at the carboxyl-terminal cytoplasmic region. Increased expression of truncated Ptc1, Ptc2 and Gli1, the indicators of ectopic activation of Hh signaling, was observed in epididymal fat pads of adult Ptc1(mes/mes) mice. In contrast, expression of peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein alpha, adipocyte P2 and adipsin were reduced in epididymal fat pads of adult Ptc1(mes/mes) mice. Taken together, our results indicate that deletion of carboxyl-terminal tail of Ptc1 can lead to the reduction of white fat mass during postnatal development.
Li
Zili
Z
Birth Defects Center, Department of Molecular, Cellular, Craniofacial Biology, University of Louisville Health Sciences Center, Louisville, KY 40202, USA.
Zhang
Heng
H
Denhard
Leslie A
LA
Liu
Lan-Hsin
LH
Zhou
Huaxin
H
Lan
Zi-Jian
ZJ
D050156:Adipogenesis / Q000235:genetics* / Q000502:physiology; D000273:Adipose Tissue / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000502:physiology*; D000818:Animals; D001823:Body Composition; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D011416:Complement Factor D / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D017353:Gene Deletion; D053823:Hedgehog Proteins / Q000378:metabolism; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*
D022763:CCAAT-Enhancer-Binding Protein-alpha; D017931:DNA Primers; D053823:Hedgehog Proteins; D047495:PPAR gamma; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; C000606240:Ptch1 protein, mouse; D011956:Receptors, Cell Surface; D011416:Complement Factor D; C493491:complement factor D, mouse