title: Anti-listerial activity of a polymeric film coated with hybrid coatings doped with Enterocin 416K1 for use as bioactive food packaging.
abstract: In this study, Enterocin 416K1, a bacteriocin produced by Enterococcus casseliflavus IM 416K1, was entrapped in an organic-inorganic hybrid coating applied to a LDPE (low-density polyethylene) film for its potential use in the active food packaging field. The antibacterial activity of the coated film was evaluated against Listeria monocytogenes NCTC 10888 by qualitative modified agar diffusion assay, quantitative determination in listeria saline solution suspension and direct contact with artificially contaminated food samples (frankfurters and fresh cheeses) stored at room and refrigeration temperatures. All investigations demonstrated that enterocin-activated coatings have a good anti-listeria activity. Qualitative tests showed a clear zone of inhibition in the indicator lawn in contact with and around the coated film. During the quantitative antibacterial evaluation the L. monocytogenes viable counts decreased to 1.5 log units compared to the control. The inhibitory capability was confirmed also in food-contact assays. In all food samples packed with coated films we observed a significant decrease in L. monocytogenes viable counts in the first 24 h compared to the control. This difference was generally maintained up to the seventh day and then decreased, with the exception of the cheese samples stored at refrigeration temperature.
authors.affiliation: Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41100 Modena, Italy.
authors.forename: Ramona
authors.initials: R
authors.lastname: Iseppi
authors.forename: Francesco
authors.initials: F
authors.lastname: Pilati
authors.forename: Michele
authors.initials: M
authors.lastname: Marini
authors.forename: Maurizio
authors.initials: M
authors.lastname: Toselli
authors.forename: Simona
authors.initials: S
authors.lastname: de Niederhäusern
authors.forename: Elisa
authors.initials: E
authors.lastname: Guerrieri
authors.forename: Patrizia
authors.initials: P
authors.lastname: Messi
authors.forename: Carla
authors.initials: C
authors.lastname: Sabia
authors.forename: Giuliano
authors.initials: G
authors.lastname: Manicardi
authors.forename: Immacolata
authors.initials: I
authors.lastname: Anacarso
authors.forename: Moreno
authors.initials: M
authors.lastname: Bondi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D016983:Enterococcus / Q000378:metabolism*; D005506:Food Contamination / Q000517:prevention & control; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods*; D005519:Food Preservation / Q000379:methods; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D001952:Bridged-Ring Compounds; C012306:enterocin