GeneXpert enterovirus assay: one-year experience in a routine laboratory setting and evaluation on three proficiency panels.
A prospective unblinded comparative evaluation of three assays for the detection of enteroviral RNA performed on 83 positive and 79 negative cerebrospinal fluid samples showed initial and resolved sensitivities of 90.4% and 98.8%, respectively, for the Cepheid GeneXpert enterovirus assay; 94.0% and 97.6%, respectively, for the Argene enterovirus consensus kit; and 100% and 100%, respectively, for an in-house real-time PCR. The initial and resolved specificities were 100% for all three assays.
Seme
Katja
K
Institute of Microbiology and Immunology, Faculty of Medicine, Zaloska 4, Ljubljana 1000, Slovenia. mario.poljak@mf.uni-lj.si.
Mocilnik
Tina
T
Komlos
Kristina Fujs
KF
Doplihar
Ana
A
Persing
David H
DH
Poljak
Mario
M
D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000821:virology; D006801:Humans; D008587:Meningitis, Viral / Q000175:diagnosis* / Q000821:virology*; D012367:RNA, Viral / Q000134:cerebrospinal fluid*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic