The occurrence of HR1b in the venom of the snake Okinawa habu (Protobothrops flavoviridis).
This study describes the first isolation of hemorrhagic metalloproteinase HR1b from the venom of Okinawa habu, and its cDNA cloning. The substrate specificity of isolated HR1b definitely differed from that of HR1a, further supporting the presence of a metalloproteinase distinguishable from HR1a in the venom of Okinawa habu. The deduced amino acid sequence of HR1b showed 99.67% identity with HR1b of Amami habu, with only two amino acid residue replacements.
Morine
Nobuya
N
United Graduate School of Agricultural Sciences, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan. morinenb@pref.okinawa.lg.jp
Matsuda
Seiko
S
Terada
Koki
K
Iwasaki
Hironori
H
Oku
Hirosuke
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017837:Bothrops; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000201:enzymology*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D045726:Metalloproteases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data
D003435:Crotalid Venoms; D017931:DNA Primers; D045726:Metalloproteases