title: Effect of temperature on pharmacokinetics of enrofloxacin in mud crab, Scylla serrata (Forsskål), following oral administration.
abstract: The study was conducted to evaluate the pharmacokinetics of enrofloxacin following a single oral gavage (10 mg kg(-1)) in mud crab, Scylla serrata, at water temperatures of 19 and 26 degrees C. Enrofloxacin concentration in haemolymph was determined using high-performance liquid chromatography (HPLC). A multiple and repeated haemolymph sampling from the articular cavity of crab periopods was developed. The haemolymph of an individual crab was successfully sampled up to 11 times from the articular cavity. The profile of haemolymph enrofloxacin concentration of an individual crab versus time was thus achieved. The mean haemolymph enrofloxacin concentration versus time was described by a two-compartment model with first-order absorption at two water temperatures. The peak concentrations of haemolymph enrofloxacin at 19 and 26 degrees C were 7.26 and 11.03 mug mL(-1), at 6 and 2 h, respectively. The absorption and distribution half-life time ( and t(1/2alpha)) at 19 degrees C were 3.7 and 4.5 h, respectively, which were markedly larger than the corresponding values (1.1 and 1.5 h) at 26 degrees C; the elimination half-life time (t(1/2beta)) was 79.1 and 56.5 h at 19 and 26 degrees C, respectively. The area under curve (AUC), total body clearance (Cl) and mean residence time (MRT(0-infinity)) at 19 degrees C were 636.0 mg L(-1) h, 0.016 L h(-1) kg(-1) and 102.5 h, respectively; the corresponding values at 26 degrees C were 583.4 mg L(-1) h, 0.018 L h(-1) kg(-1)and 63.7 h. These results indicate that enrofloxacin is absorbed and eliminated more rapidly in mud crab at 26 degrees C than at 19 degrees C.
authors.affiliation: East China Sea Fisheries Research Institute, Chinese Academy of Fisheries Science, Key and Open Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, 300 Jungong Road, Shanghai, China. whfang@sh163.net
authors.forename: W H
authors.initials: WH
authors.lastname: Fang
authors.forename: L L
authors.initials: LL
authors.lastname: Hu
authors.forename: X L
authors.initials: XL
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: Hu
authors.forename: S C
authors.initials: SC
authors.lastname: Liang
authors.forename: S
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003386:Brachyura / Q000502:physiology*; D000077422:Enrofloxacin; D024841:Fluoroquinolones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D006207:Half-Life; D006458:Hemolymph / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D000077422:Enrofloxacin