title: Molecular typing of Australian Scedosporium isolates showing genetic variability and numerous S. aurantiacum.
abstract: One hundred clinical isolates from a prospective nationwide study of scedosporiosis in Australia (2003-2005) and 46 additional isolates were genotyped by internal transcribed spacer-restriction fragment length polymorphism (ITS-RFLP) analysis, ITS sequencing, and M13 PCR fingerprinting. ITS-RFLP and PCR fingerprinting identified 3 distinct genetic groups. The first group corresponded to Scedosporium prolificans (n = 83), and the other 2 comprised isolates previously identified as S. apiospermum: one of these corresponded to S. apiospermum (n = 33) and the other to the newly described species S. aurantiacum (n = 30). Intraspecies variation was highest for S. apiospermum (58%), followed by S. prolificans (45%) and S. aurantiacum (28%) as determined by PCR fingerprinting. ITS sequence variation of 2.2% was observed among S. apiospermum isolates. No correlation was found between genotype of strains and their geographic origin, body site from which they were cultured, or colonization versus invasive disease. Twelve S. prolificans isolates from 2 suspected case clusters were examined by amplified fragment length polymorphism analysis. No specific clusters were confirmed.
authors.affiliation: Westmead Hospital, Westmead, New South Wales, Australia.
authors.forename: Laurence
authors.initials: L
authors.lastname: Delhaes
authors.forename: Azian
authors.initials: A
authors.lastname: Harun
authors.forename: Sharon C A
authors.initials: SC
authors.lastname: Chen
authors.forename: Quoc
authors.initials: Q
authors.lastname: Nguyen
authors.forename: Monica
authors.initials: M
authors.lastname: Slavin
authors.forename: Christopher H
authors.initials: CH
authors.lastname: Heath
authors.forename: Krystyna
authors.initials: K
authors.lastname: Maszewska
authors.forename: Catriona
authors.initials: C
authors.lastname: Halliday
authors.forename: Vincent
authors.initials: V
authors.lastname: Robert
authors.forename: Tania C
authors.initials: TC
authors.lastname: Sorrell
authors.forename: Wieland
authors.initials: W
authors.lastname: Meyer
mesh_terms: D001315:Australia / Q000453:epidemiology; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008271:Mycetoma / Q000453:epidemiology* / Q000382:microbiology; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D021681:Scedosporium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer