title: Validation of the BrockTB stat-pak assay for detection of tuberculosis in Eurasian badgers (Meles meles) and influence of disease severity on diagnostic accuracy.
abstract: A lateral-flow immunoassay (BrockTB Stat-Pak) for detecting tuberculosis in Eurasian badgers was 49% sensitive and 93% specific against culture for M. bovis (n = 1,464) at necropsy. However, the sensitivity was significantly higher (66 to 78%) in animals with more severe tuberculosis, indicating that the BrockTB Stat-Pak may be useful for the detection of badgers with the greatest risk of transmitting disease.
authors.affiliation: TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency Weybridge, New Haw, Surrey KT15 3NB, United Kingdom. m.a.chambers@vla.defra.gsi.gov.uk.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Chambers
authors.forename: Tim
authors.initials: T
authors.lastname: Crawshaw
authors.forename: Sue
authors.initials: S
authors.lastname: Waterhouse
authors.forename: Richard
authors.initials: R
authors.lastname: Delahay
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
authors.forename: Konstantin P
authors.initials: KP
authors.lastname: Lyashchenko
mesh_terms: D000818:Animals; D003470:Culture Media; D007118:Immunoassay / Q000379:methods*; D045969:Mustelidae / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014376:Tuberculosis / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*
chemical_list: D003470:Culture Media; D011933:Reagent Kits, Diagnostic