title: The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus.
abstract: The p24 family members are transmembrane proteins assembled into heteromeric complexes that continuously cycle between the ER and the Golgi apparatus. These cargo proteins were assumed to play a structural role in COPI budding because of their major presence in mammalian COPI vesicles. However, this putative function has not been proved conclusively so far. Furthermore, deletion of all eight yeast p24 family members does not produce severe transport phenotypes, suggesting that the p24 complex is not essential for COPI function. In this paper we provide direct evidence that the yeast p24 complex plays an active role in retrograde transport from Golgi to ER by facilitating the formation of COPI-coated vesicles. Therefore, our results demonstrate that p24 proteins are important for vesicle formation instead of simply being a passive traveler, supporting the model in which cargo together with a small GTPase of the ARF superfamily and coat subunits act as primer for vesicle formation.
authors.affiliation: Department of Cell Biology. University of Seville, 41012 Seville, Spain.
authors.forename: Auxiliadora
authors.initials: A
authors.lastname: Aguilera-Romero
authors.forename: Joanna
authors.initials: J
authors.lastname: Kaminska
authors.forename: Anne
authors.initials: A
authors.lastname: Spang
authors.forename: Howard
authors.initials: H
authors.lastname: Riezman
authors.forename: Manuel
authors.initials: M
authors.lastname: Muñiz
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism; D022181:COP-Coated Vesicles / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D017353:Gene Deletion; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances / Q000378:metabolism; D008958:Models, Molecular; D010641:Phenotype; D021381:Protein Transport / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C093262:EMP24 protein, S cerevisiae; C102179:ERV25 protein, S cerevisiae; D046911:Macromolecular Substances; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D020823:ADP-Ribosylation Factor 1