title: Molecular identification and characterization of peptide: N-glycanase from Schizosaccharomyces pombe.
abstract: Peptide:N-glycanase (PNGase) is an enzyme responsible for deglycosylation of misfolded glycoproteins in so-called endoplasmic reticulum-associated degradation (ERAD) system. In this study, we reported the molecular identification and characterization of SpPNGase (Schizosaccharomyces pombe PNGase). Enzymatic analysis revealed that SpPNGase deglycosylated the misfolded glycoproteins and distinguished native and denatured high-mannose glycoproteins in vitro. The deglycosylation activity was lost with the addition of chelating agent EDTA and was not restored by re-addition of metal ions. By construction of deletion mutant, we confirmed that N-terminal alpha-helix of SpPNGase was responsible for the protein-protein interaction. Combining the results from ternary structure prediction and dendrogram analysis, we suggested that the N-terminal alpha-helices of PNGase are derived from evolutionary motif/peptide fusion.
authors.affiliation: State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Shanda Nanlu 27, Jinan, Shandong 250100, China.
authors.forename: Fengxue
authors.initials: F
authors.lastname: Xin
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.forename: Quanfeng
authors.initials: Q
authors.lastname: Liang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D019143:Evolution, Molecular; D008958:Models, Molecular; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D012260:Ribonucleases / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology*; D020349:Surface Plasmon Resonance
chemical_list: D012260:Ribonucleases; C029600:ribonuclease B; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase