Rapid and quantitative DNA analysis of genetic mutations for polycystic kidney disease (PKD) using magnetic/luminescent nanoparticles.
Rapid and accurate detection of genetic mutations based on nanotechnology would provide substantial advances in detection of polycystic kidney disease (PKD), a disease whose current methods of detection are cumbersome due to the large size and duplication of the mutated gene. In this study, a nanotechnology-based DNA assay was developed for detection of SNPs (single nucleotide polymorphisms) in a feline autosomal dominant PKD (ADPKD) model which can readily be adapted to diagnosis of human ADPKD type 1. Europium and terbium phosphors were doped into gadolinium crystal hosts with a magnetic core, providing stable luminescence and the possibility of magnetic manipulations in a solution-based assay. A hybridization-in-solution DNA assay was optimized for feline PKD gene SNP detection using genomic DNA extracted from feline kidney tissue and blood. This assay showed a substantial differentiation between PKD and control specimens. The nanotechnology-based DNA assay is attractive from the viewpoint of rapid availability, simple methodology, and cost reduction for clinical use to detect mutations involved in human ADPKD and other genetic diseases.
Son
Ahjeong
A
Department of Land, Air, and Water Resources, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA.
Dhirapong
Amy
A
Dosev
Dosi K
DK
Kennedy
Ian M
IM
Weiss
Robert H
RH
Hristova
Krassimira R
KR
D000818:Animals; D002415:Cats; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry; D004195:Disease Models, Animal; D005799:Genes, Dominant; D049449:Luminescence; D008280:Magnetics; D009154:Mutation; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D009693:Nucleic Acid Hybridization; D007690:Polycystic Kidney Diseases / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
D015342:DNA Probes; D004247:DNA