title: Evolved CYP102A1 (P450BM3) variants oxidise a range of non-natural substrates and offer new selectivity options.
abstract: The evolution of CYP102A1 variants with enhanced activity and altered specificity characteristics.
authors.affiliation: Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, Oxford, UK OX1 3QR.
authors.forename: Christopher J C
authors.initials: CJ
authors.lastname: Whitehouse
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Bell
authors.forename: Henry G
authors.initials: HG
authors.lastname: Tufton
authors.forename: Richard J P
authors.initials: RJ
authors.lastname: Kenny
authors.forename: Lydia C I
authors.initials: LC
authors.lastname: Ogilvie
authors.forename: Luet-Lok
authors.initials: LL
authors.lastname: Wong
mesh_terms: D001410:Bacillus megaterium / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001555:Benzene Derivatives / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D019020:Directed Molecular Evolution; D004789:Enzyme Activation; D006899:Mixed Function Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D015394:Molecular Structure; D016296:Mutagenesis; D009251:NADPH-Ferrihemoprotein Reductase; D009281:Naphthalenes / Q000737:chemistry; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D001555:Benzene Derivatives; D009281:Naphthalenes; C024268:n-propylbenzene; C031721:naphthalene; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases