title: Antibodies to S100 proteins have anxiolytic-like activity at ultra-low doses in the adult rat.
abstract: S100 proteins are small calcium-binding proteins interacting with numerous intra- and extra cellular targets involved in diverse physiological functions. In particular, S100 proteins may be involved in the regulation of anxiety-related behaviour. In the present study, the effects of affinity-purified antibodies to S100 proteins administered orally at ultra-low doses were evaluated in pre-clinical tests for anxiolytic-like activity in the adult rat. In the Vogel conflict test in the rat, antibodies to S100 proteins increased punished drinking (anti-conflict effect) at 5 and 7.5 mL kg(-1), but not at 2.5 or 10 mL kg(-1). Antibodies to S100 proteins increased the percentage of entries into the open arms of an elevated plus-maze at 10 mL kg(-1), but not at lower doses. Taken together, these results indicate the presence of anxiolytic-like activity for antibodies to S100 proteins over the dose range 5-10 mL kg(-1) in the adult rat.
authors.affiliation: Porsolt & Partners Pharmacology, 9 bis rue Henri Martin, 92100 Boulogne-Billancourt, France. vcastagne@porsolt.com
authors.forename: Vincent
authors.initials: V
authors.lastname: Castagne
authors.forename: Martine
authors.initials: M
authors.lastname: Lemaire
authors.forename: Irina
authors.initials: I
authors.lastname: Kheyfets
authors.forename: Julia L
authors.initials: JL
authors.lastname: Dugina
authors.forename: Svetlana A
authors.initials: SA
authors.lastname: Sergeeva
authors.forename: Oleg I
authors.initials: OI
authors.lastname: Epstein
mesh_terms: D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000906:Antibodies / Q000008:administration & dosage / Q000494:pharmacology*; D001007:Anxiety / Q000188:drug therapy*; D003220:Conflict, Psychological; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005106:Exploratory Behavior / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000276:immunology*
chemical_list: D014151:Anti-Anxiety Agents; D000906:Antibodies; D009418:S100 Proteins