title: Expression and purification of recombinant proteins using the baculovirus system.
abstract: This unit describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production , how to maximize and scale up recombinant protein production, and how to purify recombinant proteins.
authors.affiliation: Aquila Biopharmaceuticals, Worcester, Massachusetts, USA.
authors.forename: Cheryl Isaac
authors.initials: CI
authors.lastname: Murphy
authors.forename: Helen
authors.initials: H
authors.lastname: Piwnica-Worms
authors.forename: Stefan
authors.initials: S
authors.lastname: Grünwald
authors.forename: William G
authors.initials: WG
authors.lastname: Romanow
authors.forename: Nicole
authors.initials: N
authors.lastname: Francis
authors.forename: Hua-Ying
authors.initials: HY
authors.lastname: Fan
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D002460:Cell Line / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics*; D005982:Glutathione Transferase / Q000032:analysis; D006639:Histidine / Q000032:analysis; D007202:Indicators and Reagents; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004274:DNA, Recombinant; D007202:Indicators and Reagents; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C033223:polyhistidine; D006639:Histidine; D005982:Glutathione Transferase