A sensitive resonance scattering spectral assay for the determination of trace H2O2 based on the hrp catalytic reaction and nanogold aggregation.
Gold nanoparticles in size of 15 nm exhibit a resonance scattering (RS) peak at 580 nm, in pH 6.4 citrate buffer solutions. Horseradish peroxidase (HRP) strongly catalyzed the H2O2 oxidation of o-phenylenediamine to form an intergradation of cyclohexa-3, 5-diene-1, 2-diylidenediamine and the product of 2, 3-diaminophenazin. Both cause gold nanoparticles aggregations to enhance the RS intensity at 580 nm greatly. Under the optimal conditions, the concentration of H2O2 (C) in the range of 0.08-2.2 micromol/l was proportional to the enhanced RS intensity at 580 nm (DeltaI580 nm). Its regression equation was DeltaI580 nm=46.8C+3.4, with a correlation coefficient of 0.9983 and a detection limit of 0.03 micromol/l H2O2. This new RS spectral method was applied to the determination of H2O2 in water samples with satisfactory results.
Liang
Aihui
A
Key Laboratory of New Processing Technology for Nonferrous Metals and Materials of Education Ministry, Guilin University of Technology, Guilin, 541004, China. ahliang@glite.edu.cn
Zhang
Nannan
N
Jiang
Zhiliang
Z
Wang
Sumei
S
D002384:Catalysis; D017612:Gold Compounds / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D010619:Phenazines / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry; D012542:Scattering, Radiation; D013053:Spectrophotometry
D017612:Gold Compounds; D010619:Phenazines; D010655:Phenylenediamines; C054312:2,3-diaminophenazine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase