Evaluation of the Abbott RealTime HBV DNA assay and comparison to the Cobas AmpliPrep/Cobas TaqMan 48 assay in monitoring patients with chronic cases of hepatitis B.
The new Abbott RealTime hepatitis B virus (HBV) assay was compared to the Cobas AmpliPrep/Cobas TaqMan assay with 128 serum samples from patients with chronic hepatitis B. There was an excellent correlation (r = 0.961) between the two assays, with the Abbott RealTime test showing at least equivalent sensitivity and a slightly wider dynamic range than the Cobas TaqMan assay. By coupling high sensitivity with a large dynamic range, the Abbott RealTime HBV assay is useful in monitoring the response to antiviral therapy.
Ciotti
Marco
M
Laboratory of Clinical Microbiology and Virology, University Hospital Tor Vergata, Viale Oxford, 81-00133 Rome, Italy. marco.ciotti@ptvonline.it
Marcuccilli
Fabbio
F
Guenci
Tania
T
Prignano
Maria Grazia
MG
Perno
Carlo Federico
CF
D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D016014:Linear Models; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019914:Taq Polymerase
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic; D019914:Taq Polymerase