title: Plating lambda phage to generate plaques.
abstract: This unit provides detailed protocols for isolating a single plaque by titering serial dilutions or streaking on a lawn of cells. A discussion of phage transfection and in vitro packaging is also included.
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Lech
authors.forename: R
authors.initials: R
authors.lastname: Brent
mesh_terms: D010582:Bacteriophage lambda / Q000254:growth & development*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000821:virology*; D008967:Molecular Biology / Q000379:methods*; D010948:Viral Plaque Assay / Q000379:methods*