Secondary metabolite profiling of Alternaria dauci, A. porri, A. solani, and A. tomatophila.
Chemotaxonomy (secondary metabolite profiling) has been shown to be of great value in the classification and differentiation in Ascomycota. However, few studies have investigated the use of metabolite production for classification and identification purposes of plant pathogenic Alternaria species. The purpose of the present study was to describe the methodology behind metabolite profiling in chemotaxonomy using A. dauci, A. porri, A. solani, and A. tomatophila strains as examples of the group. The results confirmed that A. dauci, A. solani, and A. tomatophila are three distinct species each with their own specific metabolite profiles, and that A. solani and A. tomatophila both produce altersolanol A, altertoxin I, and macrosporin. By using automated chemical image analysis and other multivariate statistic analyses, three sets of species-specific metabolites could be selected, one each for A. dauci, A. solani, and A. tomatophila.
Andersen
Birgitte
B
The Mycology Group, Center for Microbial Biotechnology, BioCentrum-DTU, Building 221, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. ba@biocentrum.dtu.dk
Dongo
Anita
A
Pryor
Barry M
BM
D000528:Alternaria / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D016000:Cluster Analysis; D015999:Multivariate Analysis; D016533:Mycological Typing Techniques; D009183:Mycotoxins / Q000032:analysis / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D013045:Species Specificity; D014675:Vegetables / Q000382:microbiology
D015415:Biomarkers; D009183:Mycotoxins