Recognition of T.A interruption by 2',4'-BNAs bearing heteroaromatic nucleobases through parallel motif triplex formation.
2'-O,4'-C-methylene bridged nucleic acid (2',4'-BNA) monomers bearing novel unnatural nucleobases, 4-(3-benzamidophenyl)-2-pyridone and 2-(N-methylbenzamido)thiazole, were synthesized and successfully incorporated into oligonucleotides. UV melting experiments showed that the corresponding oligonucleotide derivatives formed stable triplexes with dsDNA targets even in the presence of a T.A interruption.
Obika
Satoshi
S
Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Inohara
Hiroyasu
H
Hari
Yoshiyuki
Y
Imanishi
Takeshi
T
D020029:Base Pairing; D001549:Benzamides / Q000737:chemistry*; D001952:Bridged-Ring Compounds; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*
D001549:Benzamides; D001952:Bridged-Ring Compounds; D009696:Nucleic Acids; D009841:Oligonucleotides