Ferrocenyl glycopeptides as electrochemical probes to detect autoantibodies in multiple sclerosis patients' sera.
Glycopeptide analogues of CSF114(Glc), modified at N-terminus with new ferrocenyl carboxylic acid and a new ferrocenyl-thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these "electrochemical probes" did not affect autoantibody recognition both in SP-ELISA and in inhibition experiments. By electrochemical monitoring the interactions of the modified peptides Fc-CSF114(Glc) and 4-FcPhP(S)Abu-CSF114(Glc) with the autoantibodies, we demonstrated that autoantibodies could be detected with a sensitivity comparable to ELISA method. The new electrochemical probes can be proposed to characterize autoantibodies as biomarkers of multiple sclerosis by a simple, rapid, and reproducible cyclic voltammetry-based diagnostic methodology.
Real-Fernández
Feliciana
F
Laboratory of Peptide and Protein Chemistry and Biology, Polo Scientifico e Tecnologico, Department of Organic Chemistry Ugo Schiff and CNR ICCOM, University of Florence, and Azienda Ospedaliera Careggi, Firenze, Italy.
Colson
Amélie
A
Bayardon
Jérome
J
Nuti
Francesca
F
Peroni
Elisa
E
Meunier-Prest
Rita
R
Lolli
Francesco
F
Chelli
Mario
M
Darcel
Christophe
C
Jugé
Sylvain
S
Papini
Anna Maria
AM
D000941:Antigens; D001323:Autoantibodies / Q000097:blood*; D002853:Chromatography, Liquid; D004563:Electrochemistry; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism*; D006020:Glycopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D006046:Gold; D006801:Humans; D007124:Immunoenzyme Techniques; D000075163:Metallocenes; D015335:Molecular Probes / Q000378:metabolism*; D009103:Multiple Sclerosis / Q000097:blood*; D012996:Solutions
D000941:Antigens; D001323:Autoantibodies; D005296:Ferrous Compounds; D006020:Glycopeptides; D000075163:Metallocenes; D015335:Molecular Probes; D012996:Solutions; D006046:Gold; C004998:ferrocene