title: Assembly of nucleosomal templates by salt dialysis.
abstract: Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient), which are accompanied by protocols for purification of core histones from bacteria and preparation of DNA for the nucleosomal arrays. Electrophoretic procedures to analyze the reconstituted complexes are also included.
authors.affiliation: Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Lee
authors.forename: G
authors.initials: G
authors.lastname: Narlikar
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D003956:Dialysis / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D006657:Histones / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry*; D011189:Potassium Chloride; D012965:Sodium Chloride
chemical_list: D006657:Histones; D009707:Nucleosomes; D012965:Sodium Chloride; D011189:Potassium Chloride; D004247:DNA