Site-specific covalent labeling of proteins inside live cells using small molecule probes.
The study of dynamic movement and interactions of proteins inside living cells in real time is critical for a better understanding of cellular mechanisms and functions in molecular detail. Genetically encoded fusions to fluorescent protein(s) (FP) have been widely used for this purpose [Annu. Rev. Biochem. 1998, 67, 509-544]. To obviate some of the drawbacks associated with the use of FPs [Curr. Opin. Biotechnol. 2005, 16, 1-6; Nat. Methods2006, 3, 591-596], we report a small molecule-based approach that exploits the unique reactivity between the cysteine residue at the N-terminus of a target protein and cell-permeable, thioester-based small molecule probes resulting in site-specific, covalent tagging of proteins. This approach has been demonstrated by the in vivo labeling of proteins in both bacterial and mammalian systems thereby making it potentially useful for future bioimaging applications.
Chattopadhaya
Souvik
S
Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore.
Srinivasan
Rajavel
R
Yeo
Dawn S Y
DS
Chen
Grace Y J
GY
Yao
Shao Q
SQ
D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D047668:Inteins; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D005456:Fluorescent Dyes; D015335:Molecular Probes; D011993:Recombinant Fusion Proteins; D003545:Cysteine