Osteogenic abilities of bone marrow stromal cells are not defective in patients with osteonecrosis.
We investigated whether the proliferation activities and osteogenic capacities of bone marrow stromal cells (BMSCs) are depressed in patients with osteonecrosis of the femoral head (ONFH). BMSCs were isolated from fresh bone marrow of the iliac crests of 54 donors and were differentiated into osteogenic lineage in vitro. The results of 27 consecutive patients with ONFH (16 idiopathic and 11 alcohol-induced) were compared with those of 27 patients with a nonnecrotic hip disorder. The proliferative activities of BMSCs in patients with ONFH were not found to be reduced and their osteogenic capacities (alkaline phosphatase activity and calcium deposition amount) were unaltered during in vitro differentiation. Results from patients with idiopathic or alcohol-induced ONFH were similar to those of matched patients with nonnecrotic disorder. These findings suggest that the osteogenic potentials of BMSCs are not defective in patients with ONFH.
Yoo
Jeong Joon
JJ
Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul, South Korea.
Song
Won Seok
WS
Koo
Kyung-Hoi
KH
Yoon
Kang Sup
KS
Kim
Hee Joong
HJ
D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000378:metabolism; D001854:Bone Marrow Cells / Q000473:pathology* / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005271:Femur Head Necrosis / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis / Q000502:physiology*; D017154:Stromal Cells / Q000473:pathology / Q000502:physiology; D055815:Young Adult
D000469:Alkaline Phosphatase; D002118:Calcium