title: Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects.
abstract: To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappaB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4+ lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4+ cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4+ cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.
authors.affiliation: VINCA Institute of Nuclear Sciences, Belgrade, Serbia.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Adzić
authors.forename: Ana
authors.initials: A
authors.lastname: Nićiforović
authors.forename: Bozidarka
authors.initials: B
authors.lastname: Zarić
authors.forename: Zora
authors.initials: Z
authors.lastname: Nesković-Konstantinović
authors.forename: Snezana D
authors.initials: SD
authors.lastname: Spasić
authors.forename: David R
authors.initials: DR
authors.lastname: Jones
authors.forename: Marija B
authors.initials: MB
authors.lastname: Radojcić
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D002374:Catalase / Q000378:metabolism / Q000528:radiation effects; D018929:Cell Culture Techniques / Q000379:methods*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007962:Leukocytes / Q000528:radiation effects*; D016328:NF-kappa B / Q000378:metabolism / Q000528:radiation effects; D018384:Oxidative Stress; D011839:Radiation, Ionizing; D012016:Reference Values; D013482:Superoxide Dismutase / Q000378:metabolism / Q000528:radiation effects; D000099156:Superoxide Dismutase 2
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D016328:NF-kappa B; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2