title: Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes.
abstract: Hydroperoxide lyase activity was found in sugar beet leaves. Its optimum pH and temperature were, respectively, 6.7 and 22 degrees C. Under these conditions, conversion of linolenic acid 13-hydroperoxide to cis-3-hexenal with a maximum yield of 80% was reached after only 2 min. The stability of cis-3-hexenal was improved by acidifying the reaction medium. Based on these studies, a bioprocess producing green-note aldehydes in a laboratory-scale was achieved.
authors.affiliation: General and Organic Chemistry Unit, Gembloux Agricultural University, 5030, Gembloux, Belgium. rabetafika.h@fsagx.ac.be
authors.forename: Holy N
authors.initials: HN
authors.lastname: Rabetafika
authors.forename: Cédric
authors.initials: C
authors.lastname: Gigot
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fauconnier
authors.forename: Marc
authors.initials: M
authors.lastname: Ongena
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Destain
authors.forename: Patrick
authors.initials: P
authors.lastname: du Jardin
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Wathelet
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
mesh_terms: D000446:Aldehyde-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D027461:Beta vulgaris / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D006591:Hexobarbital / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008041:Linoleic Acids / Q000378:metabolism; D008042:Linolenic Acids / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology
chemical_list: D000447:Aldehydes; D008041:Linoleic Acids; D008042:Linolenic Acids; D008054:Lipid Peroxides; C025998:13-hydroperoxylinolenic acid; D003577:Cytochrome P-450 Enzyme System; D006591:Hexobarbital; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase; C032890:13-hydroperoxylinoleic acid