title: Effect of carbon sources on the induction of xylanolytic-cellulolytic multienzyme complexes in Paenibacillus curdlanolyticus strain B-6.
abstract: The effect of polymeric substances such as alpha-cellulose, birchwood xylan, corn hull, and sugarcane bagasse, and of soluble sugars such as L-arabinose, D-galactose, D-glucose, D-xylose, and cellobiose, on the induction of multienzyme complexes in a facultatively anaerobic bacterium, Paenibacillus curdlanolyticus B-6, was investigated under aerobic conditions. Cells and culture supernatants of strain B-6 grown on different carbon sources were analyzed. Cells grown on each carbon source adhered to cellulose. Hence strain B-6 cells from all carbon sources must have an essential component responsible for anchoring the cells to the substrate surfaces. Native-polyacrylamide gel electrophoresis (native-PAGE), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), zymogram analysis, and enzymatic assays indicated that many proteins having xylanolytic and cellulolytic activities from P. curdlanolyticus B-6 grown on each carbon source were produced as two multienzyme complexes in the culture supernatants. These results indicate that P. curdlanolyticus B-6 produced multienzyme complexes when grown on both polymeric and soluble sugars. The multienzyme complexes of P. curdlanolyticus B-6 consisted of the main enzymes and non-enzymatic subunits and the production of some different subunits, depending on the carbon source.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok 10150, Thailand.
authors.forename: Rattiya
authors.initials: R
authors.lastname: Waeonukul
authors.forename: Khin Lay
authors.initials: KL
authors.lastname: Kyu
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.forename: Khanok
authors.initials: K
authors.lastname: Ratanakhanokchai
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000502:physiology; D001422:Bacterial Adhesion; D002244:Carbon / Q000378:metabolism*; D002480:Cellulase / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D009097:Multienzyme Complexes / Q000096:biosynthesis*
chemical_list: D009097:Multienzyme Complexes; D002244:Carbon; D002480:Cellulase