Characterization of a subset of bone marrow-derived natural killer cells that regulates T cell activation in rats.
We report that bone marrow-derived natural killer (BMNK) cells from DA or F344 rats inhibit PMA/ionomycin-induced T cell proliferation. These NK-regulatory cells are NKR-P1A(dim), whereas a minor subpopulation is NKR-P1A(bright). Only the NKR-P1A(dim) BMNK cells inhibit T cell proliferation. If activated with rat Con A supernatant, the NKR-P1A(dim) cells become NKR-P1A(bright) and lose the ability to inhibit T cell proliferation. In contrast to BMNK cells, all DA and F344 rat NK cells isolated from the blood, spleen, cervical, or mesenteric lymph nodes or Peyer's patches are NKR-P1A(bright) and lack the ability to inhibit T cell proliferation. Inhibition of T cell proliferation correlates with significant down-regulation of CD3, suggesting that this may be the mechanism through which the NKR-P1A(dim) cells mediate suppression. The nitric oxide synthase inhibitor N(G)-monomethyl-arginine acetate-abrogated NKR-P1A(dim) cell inhibition of T cell proliferation. We conclude that rat bone marrow NKR-P1A(dim) cells represent a unique population that may play a role in maintaining immune homeostasis by regulating the clonal expansion of activated T cells.
Kheradmand
Taba
T
Department of Immunology and Microbiology, Wayne State University School of Medicine, 540 East Canfield Ave., Detroit, MI 48201, USA.
Trivedi
Prachi P
PP
Wolf
Norbert A
NA
Roberts
Paul C
PC
Swanborg
Robert H
RH
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002455:Cell Division; D006706:Homeostasis; D015759:Ionomycin / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D015626:Tissue Expansion; D019323:omega-N-Methylarginine / Q000494:pharmacology
D019323:omega-N-Methylarginine; D015759:Ionomycin