title: Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
abstract: We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
authors.affiliation: Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.
authors.forename: Micah
authors.initials: M
authors.lastname: Hamady
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Walker
authors.forename: J Kirk
authors.initials: JK
authors.lastname: Harris
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Gold
authors.forename: Rob
authors.initials: R
authors.lastname: Knight
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D005815:Genetic Code; D012329:RNA, Bacterial / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S