Generating somatic mosaicism with a Cre recombinase-microsatellite sequence transgene.
Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.
Akyol
Aytekin
A
Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher, Ann Arbor, Michigan 48109, USA.
Hinoi
Takao
T
Feng
Ying
Y
Bommer
Guido T
GT
Glaser
Thomas M
TM
Fearon
Eric R
ER
D000236:Adenoma / Q000209:etiology; D000818:Animals; D003110:Colonic Neoplasms / Q000209:etiology; D003111:Colonic Polyps / Q000209:etiology; D005260:Female; D019426:Integrases / Q000235:genetics*; D007422:Intestines / Q000201:enzymology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D018895:Microsatellite Repeats; D009030:Mosaicism; D019076:Transgenes; D001616:beta-Galactosidase / Q000378:metabolism
C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase