[Effects of house dust mite allergen DNA vaccines on the allergic airway inflammation in murine models].
OBJECTIVE
To investigate the effects of allergen DNA vaccines on the airway allergic inflammation in C57BL/6 mouse models sensitized and challenged with house dust mite extracts.


METHODS
C57BL/6 mice were sensitized and challenged with house dust mite extract (HDME). After vaccinated with Der p1 or Der p2 DNA vaccine or both, the mice were rechallenged with HDME. All mice underwent pulmonary lavage in 24h after the last time of rechallenge, and the pathological manifestations of the lung, cell counts and differentials, and IL-4, IL-5, IL-10, IFNgamma, IgE and IgG1 in broncho-alveolar fluid (BALF) and serum were detected.


RESULTS
Airway eosinophilic inflammation was evident in mice sensitized/challenged with HDME. Total cell count, eosinophil count and the levels of IL-4, IL-5, IgE and IgG1 in BALF increased, and IL-10 decreased significantly, while the IFNgamma level showed no change, as compared with the control group. In the Der p1 group, the Der p2 group, and the Der p1 and p2 group, the infiltration of inflammatory cells and the levels of IL-4, IL-5, IgE and IgG1 decreased; the Der p1 and p2 group showed no significant difference compared with the control group. The effect was more significant in the Der p1 and p2 group compared to the Der p1 group and the Der p2. IFNgamma level showed no significant change in three DNA vaccine groups.


CONCLUSIONS
Der p1 and p2 DNA vaccine, Der p1 DNA vaccine, and Der p2 DNA vaccine could efficiently reverse established allergic inflammation. Der p1 and p2 DNA vaccine was more effective than Der p1 DNA vaccine and Der p2 DNA vaccine.
Bi
Yu-tian
YT
Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China.
Wu
Kui
K
Wang
Yan
Y
Zhuo
Wen-lei
WL
Wang
Chang-zheng
CZ
Qian
Gui-sheng
GS
D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D001985:Bronchial Provocation Tests; D006967:Hypersensitivity / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D007074:Immunoglobulin G / Q000097:blood; D007249:Inflammation; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D051379:Mice; D008810:Mice, Inbred C57BL; D011014:Pneumonia / Q000097:blood / Q000188:drug therapy / Q000473:pathology; D039981:Pyroglyphidae / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology / Q000627:therapeutic use*
D039741:Antigens, Dermatophagoides; D007074:Immunoglobulin G; D015848:Interleukin-5; D019444:Vaccines, DNA; D016753:Interleukin-10; D015847:Interleukin-4