title: Interactions of acetylcholinesterase with caveolin-1 and subsequently with cytochrome c are required for apoptosome formation.
abstract: Acetylcholinesterase (AChE) is emerging as an important component in leading to apoptosis. Our previous study demonstrated that silencing of the AChE gene blocked the interaction between cytochrome c and apoptotic protease-activating factor-1 (Apaf-1) in etoposide-induced apoptosis of HT-29 cells. We undertook this study to further dissect the molecular role of AChE in apoptosome formation. The present study elicited that small interfering RNA (siRNA) to cytochrome c gene blocked the interaction of AChE with Apaf-1, whereas siRNA to Apaf-1 gene did not block the interaction of AChE with cytochrome c, indicating that the interaction of AChE with cytochrome c is required for the interaction between cytochrome c and protease-activating factor-1. We further observed that AChE is localized to caveolae via interacting with caveolin-1 during apoptosis and that the disruption of caveolae prevented apoptosome formation. These data indicate that the interactions of AChE with caveolin-1 and subsequently with cytochrome c appear to be indispensable for apoptosome formation.
authors.affiliation: Department of Anatomy and Cell Biology, Dong-A University College of Medicine and Medical Science Research Center, Busan 602-714, South Korea.
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Park
authors.forename: Seung Hun
authors.initials: SH
authors.lastname: Jeong
authors.forename: Soo-Bog
authors.initials: SB
authors.lastname: Yee
authors.forename: Tae Hyun
authors.initials: TH
authors.lastname: Kim
authors.forename: Young Hwa
authors.initials: YH
authors.lastname: Soung
authors.forename: Nam Chul
authors.initials: NC
authors.lastname: Ha
authors.forename: Nam Deuk
authors.initials: ND
authors.lastname: Kim
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Hae Rahn
authors.initials: HR
authors.lastname: Bae
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Park
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Yoo
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D001483:Base Sequence; D051242:Caveolin 1 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms; D045304:Cytochromes c / Q000235:genetics*; D017931:DNA Primers; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D013467:Sulindac / Q000494:pharmacology; D014162:Transfection
chemical_list: D051242:Caveolin 1; D017931:DNA Primers; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D013467:Sulindac; D045304:Cytochromes c; D000110:Acetylcholinesterase