Transgenic mouse production by zygote injection.
This unit describes methods for the production of transgenic mice by injection of DNA into zygotes, including fertilized-egg isolation, zygote injection, and oviduct reimplantation. Methods for the preparation of plasmid and BAC DNA suitable for microinjection are also presented.
Conner
David A
DA
Harvard Medical School, Boston, Massachusetts, USA.
D000818:Animals; D004247:DNA / Q000235:genetics; D004624:Embryo Transfer; D005260:Female; D018014:Gene Transfer Techniques; D051379:Mice; D008822:Mice, Transgenic; D008845:Microinjections / Q000379:methods; D019076:Transgenes; D015053:Zygote / Q000254:growth & development / Q000637:transplantation*
D004247:DNA