A mosaic of beach bean (Canavalia rosea) caused by an isolate of Cowpea aphid-borne mosaic virus (CABMV) in Brazil.
Beach bean (Canavalia rosea) plants showing mosaic symptoms were found at Massaguaçú beach, Caraguatatuba, Brazil. A potyvirus was found to be responsible for the symptoms, based on transmission assays and electron microscopy. A positive reaction in ELISA was obtained against cowpea aphid-borne mosaic (CABMV) antisera. Viral identity was confirmed by RT-PCR using specific primers to amplify part of the NIb and the entire CP coding region of the genome and the 3'NTR. Comparison of the amplified sequences with that of CABMV showed a nucleotide sequence identity of 97% for the CP coding region. Thus, the potyvirus from beach bean should be considered a CABMV isolate, referred to as CABMV-Cr.
Kitajima
E W
EW
Departamento de Entomologia, Fitopatologia & Zoologia Agrícola, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Caixa Postal 9, Piracicaba, 13418-900, Brazil. ewkitaji@esalq.usp.br
de Alcântara
B K
BK
Madureira
P M
PM
Alfenas-Zerbini
P
P
Rezende
J A M
JA
Zerbini
F M
FM
D001938:Brazil; D032449:Canavalia / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D046529:Microscopy, Electron, Transmission; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D017800:Potyvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D020133:Reverse Transcriptase Polymerase Chain Reaction