Mapping by multiple endonuclease digestions.
In this unit, a protocol is provided in which the DNA of interest is cleaved with a variety of restriction endonucleases, either individually or in combination, and the resulting products are separated by agarose or acrylamide gel electrophoresis. By determining the sizes of DNA fragments produced by endonuclease cleavage, the restriction map is deduced progressively from simple situations where enzymes cleave the DNA once or twice to more complex situations where cleavage occurs more frequently.
Bloch
K D
KD
Harvard Medical School, Boston, Massachusetts, USA.
D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis
D004262:DNA Restriction Enzymes