Suppression of tobacco mosaic virus-induced hypersensitive-type necrotization in tobacco at high temperature is associated with downregulation of NADPH oxidase and superoxide and stimulation of dehydroascorbate reductase.
Tissue necroses and resistance during the hypersensitive response (HR) of tobacco to tobacco mosaic virus (TMV) are overcome at temperatures above 28 degrees C and the virus multiplies to high levels in the originally resistant N-gene expressing plants. We have demonstrated that chemical compounds that generate reactive oxygen species (ROS) or directly applied hydrogen peroxide (H(2)O(2)) are able to induce HR-type necroses in TMV-inoculated Xanthi-nc tobacco even at high temperatures (e.g. 30 degrees C). The amount of superoxide (O(2)(*-)) decreased, while H(2)O(2) slightly increased in TMV- and mock-inoculated leaves at 30 degrees C, as compared with 20 degrees C. Activity of NADPH oxidase and mRNA levels of genes that encode NADPH oxidase and an alternative oxidase, respectively, were significantly lower, while activity of dehydroascorbate reductase was significantly higher at 30 degrees C, as compared with 20 degrees C. It was possible to reverse or suppress the chemically induced HR-type necrotization at 30 degrees C by the application of antioxidants, such as superoxide dismutase and catalase, demonstrating that the development of HR-type necroses indeed depends on a certain level of superoxide and other ROS. Importantly, high TMV levels at 30 degrees C were similar in infected plants, whether the HR-type necrotization developed or not. Suppression of virus multiplication in resistant, HR-producing tobacco at lower temperatures seems to be independent of the appearance of necroses but is associated with temperatures below 28 degrees C.
Király
L
L
Plant Protection Institute, Hungarian Academy of Sciences, PO Box 102, H-1525 Budapest, Hungary.
Hafez
Y M
YM
Department of Botany, Plant Pathology Branch, Faculty of Agriculture, Kafr-El-Sheikh University, Kafr-El-Sheikh, Egypt.
Fodor
J
J
Plant Protection Institute, Hungarian Academy of Sciences, PO Box 102, H-1525 Budapest, Hungary.
Király
Z
Z
Plant Protection Institute, Hungarian Academy of Sciences, PO Box 102, H-1525 Budapest, Hungary.
D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D007113:Immunity, Innate; D019255:NADPH Oxidases / Q000378:metabolism*; D009336:Necrosis; D010088:Oxidoreductases / Q000378:metabolism*; D018515:Plant Leaves / Q000821:virology; D013481:Superoxides / Q000378:metabolism*; D013696:Temperature; D014026:Nicotiana / Q000201:enzymology / Q000276:immunology / Q000821:virology*; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity*; D015854:Up-Regulation
D013481:Superoxides; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D019255:NADPH Oxidases; C020666:glutathione dehydrogenase (ascorbate)