title: Assays of protein kinases using exogenous substrates.
abstract: In studies of the regulation of specific biochemical events by reversible phosphorylation, assaying the protein kinases themselves can often lead to significant progress in understanding the mechanistic details of a system under study. This unit describes assays for a variety of protein kinases that require different conditions to detect and measure their activities: cyclic nucleotide-dependent kinases, protein kinase C and isoforms, casein kinases, Ca(2+)/calmodulin-dependent kinases, and tyrosine kinase. A protocol for in-gel assays for specific kinase activity is also provided. The unit is not meant to be a catalog of individual protein kinase assays; however, the general principles of these assays should apply to most if not all known protein kinases.
authors.affiliation: The Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: A N
authors.initials: AN
authors.lastname: Carter
mesh_terms: D000818:Animals; D001681:Biological Assay; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D011232:Chemical Precipitation; D004586:Electrophoresis; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: D011506:Proteins; D014238:Trichloroacetic Acid; D002482:Cellulose; C008881:phosphocellulose; D011494:Protein Kinases; D010751:Phosphopyruvate Hydratase