title: 5' ins/del and 3' VNTR polymorphisms in the apolipoprotein B gene in relation to lipids and coronary artery disease.
abstract: BACKGROUND
  Studies that considered apolipoprotein B (APOB) gene polymorphisms as risk factors for coronary artery disease (CAD) have reported conflicting results. We sought to analyze the association between 5' ins/del and 3' VNTR polymorphisms of APOB, lipid parameters and CAD risk.
  
  
  METHODS
  We recruited 251 patients with CAD, documented by coronary angiography, and 94 controls. Genotyping was performed by PCR. Lipids and apolipoproteins were measured.
  
  
  RESULTS
  5' ins/del (ins/ins, ins/del, del/del) and 3' VNTR (LL, SS, LS) polymorphism frequencies were significantly (p<0.05) different between controls and CAD patients. LL and del/del were significantly associated with higher levels of apolipoprotein B (apoB), total cholesterol/high-density lipoprotein cholesterol ratio and apoB/apoA-I ratio (p<0.05) and with increased risk of CAD. The odds ratio for significant coronary stenosis associated with del/del was 3.2 (95% CI 1.6-36.42) (p=0.032) and with LL was 2.2 (95% CI 1.1-5.1) (p=0.042).
  
  
  CONCLUSIONS
  The two polymorphisms exert an impact on lipid levels and contribute to the susceptibility to the development of CAD.
authors.affiliation: Biochemistry Department, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Lamia
authors.initials: L
authors.lastname: Rebhi
authors.forename: Asma
authors.initials: A
authors.lastname: Omezzine
authors.forename: Kaouther
authors.initials: K
authors.lastname: Kchok
authors.forename: Radhia
authors.initials: R
authors.lastname: Belkahla
authors.forename: Ibtihel
authors.initials: I
authors.lastname: Ben Hadjmbarek
authors.forename: Jihen
authors.initials: J
authors.lastname: Rejeb
authors.forename: Nabila
authors.initials: N
authors.lastname: Ben Rejeb
authors.forename: Naoufel
authors.initials: N
authors.lastname: Nabli
authors.forename: Amina
authors.initials: A
authors.lastname: Bibi
authors.forename: Taieb
authors.initials: T
authors.lastname: Massoud
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abdelaziz
authors.forename: Essia
authors.initials: E
authors.lastname: Boughzala
authors.forename: Ali
authors.initials: A
authors.lastname: Bouslama
mesh_terms: D000328:Adult; D001055:Apolipoproteins B / Q000097:blood / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D003324:Coronary Artery Disease / Q000097:blood / Q000235:genetics*; D023921:Coronary Stenosis / Q000097:blood / Q000235:genetics; D005838:Genotype; D006801:Humans; D054643:INDEL Mutation / Q000235:genetics*; D008055:Lipids / Q000097:blood*; D018598:Minisatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012306:Risk
chemical_list: D001055:Apolipoproteins B; D008055:Lipids