title: Expression of Ki-67, p53 and p63 proteins in keratocyst odontogenic tumours: an immunohistochemical study.
abstract: OBJECTIVE
  To investigate the immunohistochemical expression of Ki-67, p53 and p63 in Keratocyst Odontogenic Tumours (KOTs) in order to contribute to the biological profile of this tumor.
  
  
  METHODS
  Immunohistochemical technique was performed using the EnVision System in 37 cases of KOTs.
  
  
  RESULTS
  Ki-67- and p53-immunostained cells were mainly located in the suprabasal layers. p63-positive cells were found throughout the lining cystic epithelium. No difference in the immunostaining for these proteins was observed between primary and recurrent KOTs (Ki-67: P = 0.5591; p53: P = 0.9847; p63: P = 0.9127), or between KOTs associated with Nevoid Basal Cell Carcinoma Syndrome (NBCCS) and sporadic KOTs (Ki-67: P = 0.7013; p53: P = 0.3197; p63: P = 0.2427).
  
  
  CONCLUSIONS
  It is possible that biological behavior of KOTs may be related to suprabasal proliferative compartment in the cystic epithelium as observed by high levels of Ki-67, p53 and p63. In addition, p63 immunostaining may represent immaturity of keratinocytes in KOTs, and suggests that this protein may participate in the regulation of epithelial cell differentiation. Taken together, these data may favor tumorigenesis on KOTs.
authors.affiliation: Laboratory of Histopathology of the Gonçalo Moniz Research Center-Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.
authors.forename: Clarissa Araújo Silva
authors.initials: CA
authors.lastname: Gurgel
authors.forename: Eduardo Antônio Gonçalves
authors.initials: EA
authors.lastname: Ramos
authors.forename: Roberto Almeida
authors.initials: RA
authors.lastname: Azevedo
authors.forename: Viviane Almeida
authors.initials: VA
authors.lastname: Sarmento
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: da Silva Carvalho
authors.forename: Jean Nunes
authors.initials: JN
authors.lastname: dos Santos
mesh_terms: D000906:Antibodies; D002452:Cell Count; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000473:pathology; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D000906:Antibodies; D019394:Ki-67 Antigen; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins