Microscopic-observation drug susceptibility assay provides rapid and reliable identification of MDR-TB.
BACKGROUND
Liquid culture systems are more rapid and sensitive for both the detection and drug susceptibility testing (DST) of Mycobacterium tuberculosis.


METHODS
St Peter's TB Specialised Hospital and public health laboratory, Addis Ababa.


OBJECTIVE
To compare the microscopic-observation drug susceptibility (MODS) assay with the BACTEC-MGIT 960 system for isoniazid and rifampicin DST (i.e., multidrug-resistant tuberculosis [MDR-TB] identification) of M. tuberculosis.


METHODS
The evaluation was based on 58 smear- and culture-positive sputum samples from patients diagnosed in Addis Ababa, Ethiopia. BACTEC-MGIT was used as the reference standard.


RESULTS
For the detection of MDR-TB, MODS has a sensitivity, specificity and accuracy rate of respectively 95%, 100% and 98.3% (kappa 0.981, concordance 98.3%). Concurrent culture detection and DST results are obtained in a median of 9 days with MODS, while indirect DST results with BACTEC-MGIT are obtained in a median of 8 days (this does not include time to primary isolate).


CONCLUSIONS
MODS is an accurate, rapid and relatively inexpensive method for the identification of MDR-TB.
Ejigu
G S
GS
Medical Faculty, College of Health Sciences, Hawassa University, Awassa, Ethiopia. girumdvm@yahoo.com
Woldeamanuel
Y
Y
Shah
N S
NS
Gebyehu
M
M
Selassie
A
A
Lemma
E
E
D000293:Adolescent; D000328:Adult; D000995:Antitubercular Agents / Q000494:pharmacology*; D002648:Child; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis*
D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin