Overview of the baculovirus expression system.
Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.
Murphy
Cheryl Isaac
CI
Aquila Biopharmaceuticals, Worcester, Massachusetts, USA.
Piwnica-Worms
Helen
H
Grünwald
Stefan
S
Romanow
William G
WG
Francis
Nicole
N
Fan
Hua-Ying
HY
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D002460:Cell Line / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D007202:Indicators and Reagents; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology; D000077183:Occlusion Body Matrix Proteins; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D015678:Viral Structural Proteins / Q000235:genetics
D004274:DNA, Recombinant; D004279:DNA, Viral; D007202:Indicators and Reagents; D000077183:Occlusion Body Matrix Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus