A temperature-regulated Campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization.
Campylobacter jejuni is a gastrointestinal pathogen of humans but can asymptomatically colonize the avian gut. C. jejuni therefore grows at both 37 degrees C and 42 degrees C, the internal temperatures of humans and birds respectively. Microarray and proteomic studies on temperature regulation in C. jejuni strain 81-176 revealed the upregulation at 42 degrees C of two proteins, Cj0414 and Cj0415, orthologous to gluconate dehydrogenase (GADH) from Pectobacterium cypripedii. 81-176 demonstrated GADH activity, converting d-gluconate to 2-keto-d-gluconate, that was higher at 42 degrees C than at 37 degrees C. In contrast, cj0414 and cj0415 mutants lacked GADH activity. Wild-type but not cj0415 mutant bacteria exhibited gluconate-dependent respiration. Neither strain grew in defined media with d-gluconate or 2-keto-d-gluconate as a sole carbon source, revealing that gluconate was used as an electron donor rather than as a carbon source. When administered to chicks individually or in competition with wild-type, the cj0415 mutant was impaired in establishing colonization. In contrast, there were few significant differences in colonization of BALB/c-ByJ mice in single or mixed infections. These results suggest that the ability of C. jejuni to use gluconate as an electron donor via GADH activity is an important metabolic characteristic that is required for full colonization of avian but not mammalian hosts.
Pajaniappan
Mohanasundari
M
Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta, Georgia, USA.
Hall
Johanna E
JE
Cawthraw
Shaun A
SA
Newell
Diane G
DG
Gaynor
Erin C
EC
Fields
Joshua A
JA
Rathbun
Kimberly M
KM
Agee
Willie A
WA
Burns
Christopher M
CM
Hall
Stephen J
SJ
Kelly
David J
DJ
Thompson
Stuart A
SA
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002169:Campylobacter Infections / Q000382:microbiology; D016123:Campylobacter jejuni / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D015169:Colony Count, Microbial; D015180:Electrophoresis, Gel, Two-Dimensional; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005942:Gluconates / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D044043:Pectobacterium / Q000201:enzymology / Q000235:genetics; D020543:Proteome / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D005942:Gluconates; D020543:Proteome; D037521:Virulence Factors; C008229:2-ketogluconate; D010088:Oxidoreductases; C030691:gluconic acid; D010100:Oxygen