Preconditioning with endoplasmic reticulum stress ameliorates mesangioproliferative glomerulonephritis.
Accumulating evidence suggests a pathophysiologic role of endoplasmic reticulum (ER) stress in kidney disease. This study investigated the potential of therapeutic approaches targeting ER stress in the anti-Thy1 model of mesangioproliferative glomerulonephritis in rats. Immunohistochemistry and Western blotting showed a time-dependent increase in the expression of the ER stress-inducible chaperones glucose-regulated protein 78 (GRP78) and oxygen-related protein 150 in isolated glomeruli, especially in the glomerular epithelial cells and mesangial cells, after induction of anti-Thy1 nephritis. For evaluation of whether preconditioning with ER stress ameliorates the severity of disease, rats were pretreated with a subnephritogenic dose of the ER stress inducer tunicamycin or thapsigargin for 4 d before disease was induced. Although preconditioning with ER stress had no effect on the degree of disease induction, it strongly ameliorated the manifestations of disease, evidenced by marked reductions in microaneurysm formation, mesangial proliferation, and adhesion of Bowman's capsule to the glomerular tuft. This improvement in histologic damage was associated with reduced proteinuria (39.4 +/- 10.5 versus 126.1 +/- 18.1 mg/d; P < 0.01) and with attenuated increases in glucose-regulated protein 78 and oxygen-related protein 150 expression. Of note, pretreatment with tunicamycin or thapsigargin decreased the excessive ER stress-induced intracellular signaling observed in anti-Thy1 nephritis. In conclusion, preconditioning with ER stress ameliorates the severity of disease in rats with anti-Thy1 nephritis. These findings suggest the possibility of therapeutic approaches targeting ER stress in mesangioproliferative glomerulonephritis.
Inagi
Reiko
R
Division of Nephrology and Endocrinology, University of Tokyo School of Medicine, 7-3-1, Hongo Bunkyo-ku, Tokyo 113-8655, Japan. inagi-npr@umin.ac.jp
Kumagai
Takanori
T
Nishi
Hiroshi
H
Kawakami
Takahisa
T
Miyata
Toshio
T
Fujita
Toshiro
T
Nangaku
Masaomi
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D004195:Disease Models, Animal; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D004791:Enzyme Inhibitors / Q000633:toxicity; D015432:Glomerulonephritis, Membranoproliferative / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy*; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D019194:Ischemic Preconditioning; D007518:Isoantibodies / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018832:Molecular Chaperones / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology; D019284:Thapsigargin / Q000633:toxicity; D014415:Tunicamycin / Q000633:toxicity
D000900:Anti-Bacterial Agents; D000091342:Endoplasmic Reticulum Chaperone BiP; D004791:Enzyme Inhibitors; C051684:GRP78 protein, rat; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D007518:Isoantibodies; D018832:Molecular Chaperones; D011506:Proteins; C035678:anti-Thy antibody; C050087:oxygen-regulated proteins; D014415:Tunicamycin; D019284:Thapsigargin