title: Optimization and modeling of phenanthrene degradation by Mycobacterium sp. 6PY1 in a biphasic medium using response-surface methodology.
abstract: In the present paper, the degradation of phenanthrene, a model polycyclic aromatic hydrocarbon compound, by the Mycobacterium strain 6PY1 was optimized in a biphasic culture medium. The optimization and modeling were performed using the design of experiments methodology. The temperature, the silicone oil/mineral salts medium volume ratio, and the initial cell concentration, were used as the central composite design parameters. In all experiments, the phenanthrene was degraded to undetectable levels. Response surface methodology was successfully employed to derive an empirical model describing the rate and time of degradation and to deduce the optimal degradation conditions. As a result of the optimization processes, the optimal responses for the degradation rate, the volumetric degradation rate, and the 90% degradation time were estimated to be 0.172 mg h(-1), 22 mg l(-1) h(-1), and 18 h, respectively.
authors.affiliation: Laboratoire d'Electrochimie at de Physico-chimie des Matériaux et des Interfaces (LEPMI), Institut National Polytechnique de Grenoble, ENSEEG, Domaine Universitaire 38402 Saint-Martin d'Heres Cedex, France. arwa.abdelhay@lepmi.inpg.fr
authors.forename: Arwa
authors.initials: A
authors.lastname: Abdelhay
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Magnin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gondrexon
authors.forename: Stéphane
authors.initials: S
authors.lastname: Baup
authors.forename: John
authors.initials: J
authors.lastname: Willison
mesh_terms: D001673:Biodegradation, Environmental; D003470:Culture Media / Q000737:chemistry*; D008954:Models, Biological; D009161:Mycobacterium / Q000378:metabolism*; D010616:Phenanthrenes / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010616:Phenanthrenes; C031181:phenanthrene