title: Minipreps of plasmid DNA.
abstract: Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.
authors.affiliation: State University of New York, Stony Brook, New York, USA.
authors.forename: J
authors.initials: J
authors.lastname: Engebrecht
authors.forename: R
authors.initials: R
authors.lastname: Brent
authors.forename: M A
authors.initials: MA
authors.lastname: Kaderbhai
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D005822:Genetic Vectors / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000302:isolation & purification*
chemical_list: D004247:DNA