Sesamol attenuates diclofenac-induced acute gastric mucosal injury via its cyclooxygenase-independent antioxidative effect in rats.
We examined the protective effects of sesamol against acute gastric mucosal damage induced in rats by nonsteroidal anti-inflammatory drug diclofenac (DLF). We also measured the ulcer index, nitrite, iNOS, lipid peroxidation, hydroxyl radical, superoxide anion, reduced glutathione levels, prostaglandin E2, mucus, and cyclooxygenase activity in the rat mucosa. Sesamol attenuated gastric ulcer, nitrite, and iNOS in DLF-treated stomachs. Sesamol reduced mucosal lipid peroxidation and hydroxyl radical levels; however, neither DLF nor sesamol affected mucosal superoxide anion production. In addition, sesamol significantly maintained the reduced mucosal glutathione levels in DLF-treated stomachs of rats. Sesamol did not affect mucosal mucus production, but it further decreased DLF-induced mucosal prostaglandin E2 generation and cyclooxygenase activity. Therefore, sesamol might protect gastric mucosa against DLF-induced injury by inhibiting hydroxyl radical-associated lipid peroxidation. In addition, the cyclooxygenase pathway may not be involved in sesamol's gastric mucosal protection.
Hsu
Dur-Zong
DZ
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, National Cheng Kung University, Tainan, Taiwan.
Chu
Pei-Yi
PY
Li
Ya-Hui
YH
Liu
Ming-Yie
MY
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D052117:Benzodioxoles / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects*; D017665:Hydroxyl Radical; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010636:Phenols / Q000494:pharmacology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000293:injuries*
D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D052117:Benzodioxoles; D010636:Phenols; D004008:Diclofenac; D017665:Hydroxyl Radical; C025583:sesamol; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone