In vitro selection of RNA aptamers to a protein target by filter immobilization.
In vitro selection of RNA aptamers that bind to a protein target is detailed in the protocols presented in this unit. Aptamers generated from these types of selection experiments can potentially function as protein inhibitors, and are often used as diagnostic or therapeutic reagents.
Jhaveri
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SD
University of Texas, Austin, Texas, USA.
Ellington
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AD
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005374:Filtration; D007202:Indicators and Reagents; D016296:Mutagenesis; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012995:Solubility; D014158:Transcription, Genetic
D018076:DNA, Complementary; D007202:Indicators and Reagents; D011506:Proteins; D012313:RNA; D004247:DNA