Evaluation of telomerase activity in gingival fibroblasts of cyclosporine-treated patients.
Gingival overgrowth (GO) is a common side effect following administration of cyclosporin A (CsA). Various case reports have shown that squamous cell carcinomas could arise in GO induced by CsA and phenytoin. It is also known that human telomerase activated in about 90% of cancers is mainly composed of hTR, hTERT, and TPI. The aim of this study was to investigate the potential role of telomerase activity in the pathogenesis of CsA-induced GO. Included in the study were 9 patients on CsA: 4 with and 5 without GO. Gingival tissues were obtained during gingivectomy or flap procedures; gingival fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10,000 U/mL penicillin, 10 mg/mL streptomycin, 2 mmol/L l-glutamine, and 10% heat-inactivated fetal bovine serum at 37 degrees C under a humidified 95% air virgule 5% CO(2) atmosphere. Quantitative detection of hTERT mRNA was performed with the commercially available LightCycler Telo TAGGG hTERT Quantification Kit using real-time online PCR. The hTERT mRNA expression was positive in one patient, while hTERT mRNA expression was negative in the others. Because results indicated that there may be a relationship between CsA-induced GO and positive telomerase activity, detailed studies should be performed to confirm the present findings.
Biray
C
C
Ege University, School of Medicine, Department of Medical Biology, Bornova Izmir, Turkey. cigir.biray@ege.edu.tr
Nizam
N
N
Gunduz
C
C
Sonmez
S
S
Cavdar
C
C
Saglam
F
F
Atilla
K
K
Camsari
T
T
D000328:Adult; D018929:Cell Culture Techniques; D016572:Cyclosporine / Q000009:adverse effects*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology* / Q000473:pathology; D005881:Gingiva / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007166:Immunosuppressive Agents / Q000009:adverse effects; D008297:Male; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
D007166:Immunosuppressive Agents; D016572:Cyclosporine; D019098:Telomerase