title: Ribonucleases.
abstract: Ribonucleases (RNases) with different sequence specificities are used for a variety of analytical purposes, including RNA sequencing, mapping, and quantitation. One very common application for RNase A is presented in this unit and involves hydrolyzing RNA that contaminates DNA preparations. Two other commonly used RNases, RNase H and RNase T1, are also described. In addition, many commercially available RNases are sequence-specific endoribonucleases, a property has been used for enzymatic sequencing of RNA.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Struhl
mesh_terms: D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D012260:Ribonucleases