title: [The preventive effects of intraperitoneal injection of perfluorocarbon on acute lung injury in rat].
abstract: OBJECTIVE
  To evaluate the preventive effects of intraperitoneal injection of perfluorocarbon (PFC) on acute lung injury (ALI) in rat.
  
  
  METHODS
  Sixty-three male Wistar rats were randomly divided into normal control (NC) group, lipopolysaccharide (LPS) group and C8F18 group. Rats in NC group were sacrificed 2 hours after anesthesia, and in LPS group and C8F18 group rats were either treated with normal saline or C8F18 15 ml/kg intraperitoneally 48 hours before LPS challenge. ALI was reproduced by intravenous injection of 7 ml/kg LPS, and the extent of lung injury was assessed by arterial partial pressure of oxygen (PaO(2)) level, histological examination, right lung wet weight/body weight (W/D) ratio, tumor necrosis factor-alpha (TNF-alpha) level in serum and broncho alveolar lavage fluid (BALF) at 2, 4, 6 hours after injury.
  
  
  RESULTS
  PaO(2) in LPS group and C8F18 group was significant lower than that in NC group (both P<0.05). There were no difference in PaO(2) between LPS group and C8F18 group at 2, 4, 6 hours after injury (all P>0.05). Compared with NC group, TNF-alpha level in blood and BALF increased obviously in LPS group and C8F18 group (both P<0.05). There was no significant change in content of TNF-alpha in C8F18 group BALF at 6 hours was significantly lower than that in LPS group (P<0.05).
  
  
  CONCLUSIONS
  Intraperitoneal administration of C8F18 (15 ml/kg) can not attenuate pathological changes or improve PaO(2) in rats with ALI induced by LPS in a short time.
authors.affiliation: General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China. fanhaojun999@126.com
authors.forename: Hao-Jun
authors.initials: HJ
authors.lastname: Fan
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
authors.forename: Jian-Peng
authors.initials: JP
authors.lastname: Zhang
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhao
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Gao
authors.forename: You-Ning
authors.initials: YN
authors.lastname: Liu
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D005466:Fluorocarbons / Q000008:administration & dosage* / Q000627:therapeutic use; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D005466:Fluorocarbons; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha