In vitro effects of alloxan/copper combinations on lipid peroxidation, protein oxidation and antioxidant enzymes.
The in vitro effects of alloxan and the product of its reduction dialuric acid (alone or in combination with copper ions) on lipid peroxidation, carbonyl content, GSH level and antioxidant enzyme activities in rat liver and kidney have been studied. The effects of Cu2+/alloxan and Cu2+/dialuric acid were compared with those of Fe3+/alloxan and Fe3+/dialuric acid. Unlike alloxan, dialuric acid increased liver and kidney lipid peroxidation; similar effects were registered in the presence of Fe3+. In the presence of Cu2+/dialuric acid, the lipid peroxidation was strongly inhibited and vice versa--the liver protein oxidation was increased. Alloxan and dialuric acid, as well as their combinations with Fe3+ had no effect on the total GSH level. Both substances did not affect the Cu2+-induced changes in GSH level, glucose-6-phosphate dehydrogenase and gluthatione reductase activities. In contrast, Cu2+ had no effect on dialuric-acid induced changes in gluthatione peroxidase and superoxide dismutase activities. The present in vitro results, concerning the metal dependence of the effects of alloxan and dialuric acid, are a premise for in vivo study of alloxan effects in metal-loaded animals.
Alexandrova
Albena
A
Institute of Physiology, Bulgarian Academy of Sciences, 23 Acad. G. Bonchev St., 1113 Sofia, Bulgaria. a_alexandrova_bas@yahoo.com
Petrov
L
L
Kessiova
Mila
M
Kirkova
Margarita
M
D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001463:Barbiturates / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology*; D004338:Drug Combinations; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D001463:Barbiturates; D004338:Drug Combinations; D011506:Proteins; C004718:dialuric acid; D000496:Alloxan; D003300:Copper; D007501:Iron; D010088:Oxidoreductases; D005978:Glutathione