title: Synthesis and purification of oligonucleotides.
abstract: This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.
authors.affiliation: University of Texas, Austin, Texas, USA.
authors.forename: A
authors.initials: A
authors.lastname: Ellington
authors.forename: J D
authors.initials: JD
authors.lastname: Pollard
mesh_terms: D009711:Nucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D014320:Triphenylmethyl Compounds / Q000737:chemistry
chemical_list: D009711:Nucleotides; D009841:Oligonucleotides; D014320:Triphenylmethyl Compounds