title: Tractable Cre-lox system for stochastic alteration of genes in mice.
abstract: We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.
authors.affiliation: Molecular and Medical Genetics, L103, Oregon Health & Science University, 3181 Sam Jackson Park Road, Portland, Oregon 97239, USA.
authors.forename: Ashleigh J
authors.initials: AJ
authors.lastname: Miller
authors.forename: Sandra D
authors.initials: SD
authors.lastname: Dudley
authors.forename: Jen-Lan
authors.initials: JL
authors.lastname: Tsao
authors.forename: Darryl
authors.initials: D
authors.lastname: Shibata
authors.forename: R Michael
authors.initials: RM
authors.lastname: Liskay
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000375:Aging / Q000235:genetics; D000818:Animals; D053843:DNA Mismatch Repair; D045643:DNA Repair Enzymes / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D016368:Frameshift Mutation; D011905:Genes, ras / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D007422:Intestines / Q000201:enzymology; D051379:Mice; D000070976:Mismatch Repair Endonuclease PMS2; D009369:Neoplasms / Q000209:etiology / Q000235:genetics; D011995:Recombination, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase; D000251:Adenosine Triphosphatases; C499533:Pms2 protein, mouse; D000070976:Mismatch Repair Endonuclease PMS2; D045643:DNA Repair Enzymes