16S rRNA gene sequences from bacteria associated with adult Anopheles darlingi (Diptera: Culicidae) mosquitoes.
The microbial flora associated with Anopheles darlingi Root (Diptera: Culicidae), a major Neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in Brazil. Female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. The controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial DNA from all blood-fed mosquitoes. The 16S sequences of bacteria recovered, include some closely related to those found in other vector mosquitoes, including Aeromonas, Pantoea and Pseudomonas species.
Terenius
Olle
O
Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, CA 92697, USA.
de Oliveira
Caroline Dantas
CD
Pinheiro
Waleria Dasso
WD
Tadei
Wanderli Pedro
WP
James
Anthony Amade
AA
Marinotti
Osvaldo
O
D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D008297:Male; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S