title: Influence of subclinical tumor spreading on survival after curative surgery for colorectal cancer.
abstract: OBJECTIVE
  To determine epithelial cell dissemination in patients with localized colorectal cancer.
  
  
  METHODS
  Prospective observational study.
  
  
  METHODS
  Academic hospital.
  
  
  METHODS
  Two hundred twenty-two patients operated on for colorectal cancer.
  
  
  METHODS
  Epithelial cell dissemination was determined using immunohistochemistry or cytology in histologically negative lymph nodes, the peritoneal cavity, and bone marrow. Prognostic significance was determined in relation to 140 clinicopathological variables. Median follow-up was 61 months.
  
  
  RESULTS
  Of 140 patients who underwent curative surgery; 25 (17.9%) died of cancer-related causes; 10 (7.1%), of other causes; and 11 (7.8%) developed local recurrence. Tumor cells were present in the peritoneal cavity of 22% of patients, but this finding had only borderline influence on disease-free survival (P = .07). Lymph node micrometastases correlated with T category but not with survival. The presence of epithelial cells in the bone marrow was detected in 64% of patients but was not associated with tumor stage or survival. Multivariate analysis failed to identify occult tumor cell dissemination into any body compartment as an independent prognostic factor of disease-free survival.
  
  
  CONCLUSIONS
  Tumor cells disseminate into various body compartments in early stages of disease. In about two-thirds of patients, tumor cells are left in the body after so-called curative surgery. However, the presence of minimal residual disease has no independent prognostic significance in relation to established risk factors for tumor progression. Thus, other factors, such as the presence of a cellular metastatic phenotype and/or ineffective immunological response, must play an important role.
authors.affiliation: Department of Surgery, Evangelic Hospital Bielefeld, Burgsteig 13, 33617 Bielefeld, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Steinert
authors.forename: Marco
authors.initials: M
authors.lastname: Hantschick
authors.forename: Michael
authors.initials: M
authors.lastname: Vieth
authors.forename: Ingo
authors.initials: I
authors.lastname: Gastinger
authors.forename: Frank
authors.initials: F
authors.lastname: Kühnel
authors.forename: Hans
authors.initials: H
authors.lastname: Lippert
authors.forename: Marc A
authors.initials: MA
authors.lastname: Reymond
mesh_terms: D000046:Academic Medical Centers; D000328:Adult; D000368:Aged; D002423:Cause of Death; D015331:Cohort Studies; D003082:Colectomy; D015179:Colorectal Neoplasms / Q000401:mortality* / Q000473:pathology* / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D005858:Germany; D006801:Humans; D007150:Immunohistochemistry; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011336:Probability; D016016:Proportional Hazards Models; D011446:Prospective Studies; D018570:Risk Assessment; D016019:Survival Analysis; D016896:Treatment Outcome