Expression of proopiomelanocortin, proenkephalin and prodynorphin genes in porcine luteal cells.
The objective of the study was to examine the expression of the genes coding for proopiomelanocortin (POMC), proenkephalin (PENK) and prodynorphin (PDYN) in porcine luteal cells isolated from corpora lutea (CL) collected on days 3-6, 8-10 and 13-16 of the oestrous cycle. Total RNA was purified from non-incubated cells and from cells incubated for 48 h in the absence or presence of luteinising hormone (LH). The semi-quantitative RT-PCR technique, involving coamplification of the target and control cDNA (beta-actin or 18S rRNA), was used to examine gene expression. It was found that the genes coding for opioid precursors are expressed in both non-incubated and incubated porcine luteal cells representing the early, mid- and late luteal phase. In non-incubated cells, only POMC mRNA content changed during CL development, whereas the expression of PENK and PDYN genes remained relatively constant. Additionally, the treatment of cells with LH markedly affected the expression of POMC and PENK, but no influence on PDYN expression was observed. The present study indicates that porcine luteal cells may produce opioid peptides and that gene expression of their precursors (except for PDYN) may be modulated in these cells by LH. Moreover, the present results support the involvement of opioid peptides in local regulation within the CL of the pig.
Staszkiewicz
J
J
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Oczapowskiego 1A, Poland. jaroslaw.staszkiewicz@uwm.edu.pl
Skowronski
M T
MT
Siawrys
Gabriela
G
Kaminski
T
T
Krazinski
B E
BE
Plonka
K J
KJ
Wylot
B
B
Przala
Jadwiga
J
Okrasa
S
S
D000818:Animals; D002478:Cells, Cultured; D003338:Corpus Luteum / Q000166:cytology*; D003470:Culture Media / Q000737:chemistry; D004745:Enkephalins / Q000235:genetics*; D030762:Estrous Cycle; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D011333:Pro-Opiomelanocortin / Q000235:genetics*; D011374:Progesterone / Q000032:analysis; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine
D003470:Culture Media; D004745:Enkephalins; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin; D011374:Progesterone; D011333:Pro-Opiomelanocortin; C033660:preproenkephalin