title: Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304.
abstract: A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 A resolution and a data set was collected at 100 K using Cu K alpha radiation from a rotating-anode X-ray source. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 49.27, c = 106.61 A. The calculated Matthews coefficient was 3.16 A(3) Da(-1), suggesting the presence of one molecule in the asymmetric unit.
authors.affiliation: College of Life Science and Technology, Xinjiang University, Urumqi 830046, People's Republic of China.
authors.forename: Pazilat
authors.initials: P
authors.lastname: Bahti
authors.forename: Shunmei
authors.initials: S
authors.lastname: Chen
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Neil
authors.initials: N
authors.lastname: Shaw
authors.forename: Xuejun
authors.initials: X
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Chongyun
authors.initials: C
authors.lastname: Cheng
authors.forename: Gaojie
authors.initials: G
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Che
authors.forename: Abdulla
authors.initials: A
authors.lastname: Abbas
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Bi Cheng
authors.initials: BC
authors.lastname: Wang
authors.forename: Zhi Jie
authors.initials: ZJ
authors.lastname: Liu
mesh_terms: D001105:Archaea / Q000737:chemistry*; D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D016366:Open Reading Frames
chemical_list: D019843:Archaeal Proteins