title: Nitric oxide synthase inhibitor attenuates the anorexigenic effect of corticotropin-releasing hormone in neonatal chicks.
abstract: Nitric oxide (NO) is known as an orexigenic factor in the brain of mammals and mediates the feeding-stimulatory effect of other factors such as neuropeptide Y (NPY). In neonatal chicks, however, we recently reported that NO might have an anorexigenic effect and suggested that the feeding-regulatory mechanism in chicks might be different from that in mammals regarding NO. In the present study, we investigated the involvement of NO in the effect of other orexigenic and anorexigenic factors in neonatal chicks. Intracerebroventricular co-injection of N(G)-nitro-l-arginine methyl ester (l-NAME), a NO synthase inhibitor, did not affect NPY- and prolactin-releasing peptide-induced feeding behavior. On the other hand, the co-injection of l-NAME significantly attenuated the anorexigenic effect of corticotropin-releasing hormone (CRH). The anorexigenic effects of glucagon-like peptide-1, alpha-melanocyte-stimulating hormone and ghrelin were not affected by the l-NAME treatment. These results suggest that NO might mediate the anorexigenic effect of CRH in the brain of neonatal chicks.
authors.affiliation: Department of Bioresource Production Science, United Graduate School of Agricultural Sciences, Ehime University, Matsuyama 790-8566, Japan.
authors.forename: Md Sakirul Islam
authors.initials: MS
authors.lastname: Khan
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Nakano
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tachibana
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000855:Anorexia / Q000139:chemically induced*; D002645:Chickens / Q000502:physiology*; D003346:Corticotropin-Releasing Hormone; D004338:Drug Combinations; D054439:Ghrelin / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology; D007028:Hypothalamic Hormones / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009478:Neuropeptide Y / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D056690:Prolactin-Releasing Hormone; D000521:alpha-MSH / Q000494:pharmacology
chemical_list: D004338:Drug Combinations; D054439:Ghrelin; D007028:Hypothalamic Hormones; D009478:Neuropeptide Y; D009479:Neuropeptides; D056690:Prolactin-Releasing Hormone; D000521:alpha-MSH; D052216:Glucagon-Like Peptide 1; D003346:Corticotropin-Releasing Hormone; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester