title: Surface localization of the nuclear receptor CAR in influenza A virus-infected cells.
abstract: Constitutive active/androstane receptor CAR is a member of the nuclear receptors which regulate transcription of xenobiotic metabolism enzymes. CAR is usually localized in the cytosol and nucleus. Here, we found that CAR was localized at the cell surface of influenza A virus (IAV)-infected cells. Additionally, we demonstrated that expression of a viral envelope glycoprotein, either hemagglutinin (HA) or neuraminidase (NA), but not viral nucleoprotein (NP), was responsible for this localization. This report is the first demonstration of CAR at the surface of tissue culture cells, and suggests that CAR may exert the IAV infection mechanism.
authors.affiliation: Department of Pharmaco-Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and COE Program in the 21st Century, 52-1 Yada, Shizuoka 422-8526, Japan.
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Moriyama
authors.forename: Akira
authors.initials: A
authors.lastname: Ikari
authors.forename: Junko
authors.initials: J
authors.lastname: Sugatani
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Masao
authors.initials: M
authors.lastname: Miwa
mesh_terms: D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology*; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism*; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism*
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011991:Receptors, Virus