title: A novel approach obtaining intron-containing hairpin RNA constructs.
abstract: Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target genes efficiently in a variety of species. Here we designed a new recombinant-PCR mediated method called direct amplification of ihpRNA from genomic DNA. This approach has proved to be easy, stable, and efficient.
authors.affiliation: Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, PR China.
authors.forename: Chun-Miao
authors.initials: CM
authors.lastname: Wen
authors.forename: Ping-Zhong
authors.initials: PZ
authors.lastname: Cai
authors.forename: Zheng-Jun
authors.initials: ZJ
authors.lastname: Xu
authors.forename: Wan-Fang
authors.initials: WF
authors.lastname: Zhong
mesh_terms: D001483:Base Sequence; D020868:Gene Silencing; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*
chemical_list: D012313:RNA