title: Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols--a proof-of-concept study.
abstract: Three typical glycosidase-inhibiting iminoalditols were attached to a polyamine surface displayed on a silicon chip. Exposure to a representative beta-glucosidase revealed selective binding events reflecting the different structural features of the inhibitors probed in this study. This provides a proof-of-concept for the successful exploitation of microarrays of typical reversible glycosidase inhibitors of the iminosugar family.
authors.affiliation: Glycogroup, Institut für Organische Chemie,Technische Universität Graz, Stremayrgasse 16, A-8010 Graz, Austria.
authors.forename: Andreas J
authors.initials: AJ
authors.lastname: Steiner
authors.forename: Arnold E
authors.initials: AE
authors.lastname: Stütz
authors.forename: Tanja M
authors.initials: TM
authors.lastname: Wrodnigg
authors.forename: Chris A
authors.initials: CA
authors.lastname: Tarling
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Withers
authors.forename: Albin
authors.initials: A
authors.lastname: Hermetter
authors.forename: Hannes
authors.initials: H
authors.lastname: Schmidinger
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D050111:Imino Sugars / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D046228:Microarray Analysis; D015394:Molecular Structure; D012231:Rhizobium / Q000201:enzymology
chemical_list: D004791:Enzyme Inhibitors; D050111:Imino Sugars; D006026:Glycoside Hydrolases