Increased levels of serum matrix metalloproteinase-3 in haemodialysis patients with dialysis-related amyloidosis.
BACKGROUND
It is recognized that matrix metalloproteinase-3 (MMP-3) is abundantly expressed in active rheumatoid synovium, and that serum level of MMP-3 is a useful marker for diagnosis of rheumatoid arthritis and for evaluation of prognosis in joint destruction. Little is known about serum MMP-3 levels in haemodialysis (HD) patients, and thus, the association between serum MMP-3 and dialysis-related amyloidosis (DRA) has yet to be elucidated.


METHODS
Serum levels of MMP-3 were measured by enzyme immunoassay in 150 HD patients, 90 without DRA and 60 with DRA, before HD. Simple regression analysis was performed to investigate the relationship between serum level of MMP-3 and clinical parameters, including age, HD duration, C-reactive protein and beta2 microglobulin (BMG).


RESULTS
Serum levels of MMP-3 were significantly higher in HD patients with DRA than in HD patients without DRA (258.2 +/- 118.1 vs 201.5 +/- 98.4 pg/mL, P = 0.0017), and both levels were significantly higher than those of healthy subjects (45.6 +/- 13.4 pg/mL, P < 0.0001). Serum MMP-3 levels significantly correlated with serum levels of BMG (r = 0.197, P = 0.0164) and HD duration (r = 0.168, P = 0.0427). Moreover, serum MMP-3 levels significantly correlated with serum BMG levels in HD patients without DRA (r = 0.341, P = 0.0012), but not in HD patients with DRA.


CONCLUSIONS
Our results suggest that matrix metalloproteinase activity increases in HD patients, which may be associated with BMG and DRA.
Naganuma
Toshihide
T
Department of Urology, Osaka City University Medical School, Osaka, Japan. m9643361@msic.med.osaka-cu.ac.jp
Sugimura
Kazunobu
K
Uchida
Junji
J
Tashiro
Koichiro
K
Yoshimura
Rikio
R
Takemoto
Yoshiaki
Y
Nakatani
Tatsuya
T
D000368:Aged; D000686:Amyloidosis / Q000201:enzymology* / Q000209:etiology; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology / Q000628:therapy*; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000097:blood*; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects*; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D001613:beta 2-Microglobulin / Q000097:blood
D015415:Biomarkers; D001613:beta 2-Microglobulin; D002097:C-Reactive Protein; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3