title: Dehydroabietic acid, a phytochemical, acts as ligand for PPARs in macrophages and adipocytes to regulate inflammation.
abstract: Obesity is characterized by an enhanced infiltration of macrophages to adipose tissues, which is closely associated with the low-grade inflammatory state and obesity-related pathologies such as type 2 diabetes and cardiovascular diseases. We showed here that dehydroabietic acid (DAA) is a potent PPARalpha/gamma dual activator. Furthermore, we examined the anti-inflammatory effects of DAA in stimulated macrophages and in the coculture of macrophages and adipocytes. DAA significantly suppressed the production of proinflammatory mediators such as MCP-1, TNF-alpha, and NO in stimulated RAW 264 macrophages and in the coculture of RAW 264 macrophages and 3T3-L1 adipocytes. These results suggest that DAA is a valuable medicinal and food component for improving inflammatory changes associated with obesity-related diabetes.
authors.affiliation: Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Min-Sook
authors.initials: MS
authors.lastname: Kang
authors.forename: Shizuka
authors.initials: S
authors.lastname: Hirai
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.forename: Kayo
authors.initials: K
authors.lastname: Kuroyanagi
authors.forename: Joo-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Taku
authors.initials: T
authors.lastname: Uemura
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ezaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
mesh_terms: D041721:3T3-L1 Cells; D045784:Abietanes / Q000008:administration & dosage*; D017667:Adipocytes / Q000187:drug effects / Q000276:immunology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D002460:Cell Line; D007249:Inflammation / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D008024:Ligands; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D047493:PPAR alpha / Q000276:immunology*; D047495:PPAR gamma / Q000276:immunology*
chemical_list: D045784:Abietanes; D000893:Anti-Inflammatory Agents; D008024:Ligands; D047493:PPAR alpha; D047495:PPAR gamma; C013913:dehydroabietic acid