Determination of 4-ethylcatechol in wine by high-performance liquid chromatography-coulometric electrochemical array detection.
A HPLC method using a coulometric electrode array detector (CEAD) to analyse 4-ethylcatechol in wine was established. The procedure does not require any sample preparation or analyte derivatisation and performs chromatographic separation in a short time. The assay method is linear up to 1520microgL(-1) and precise (R.S.D.<3%), with limits of detection and quantitation of 1.34microgL(-1) and 2.2microgL(-1), respectively. Recoveries in spiked wine samples ranged from 95% to 104% with a median value of 102% and matrix effects were not observed. The method was applied to the evaluation of the concentration of 4-EC in 250 commercial Italian wines. The red wines analysed had median, 75 degrees percentile and maximum values of 37microgL(-1), 89microgL(-1) and 1610microgL(-1), respectively. For Sangiovese-based wines the mean ratios of 4-EP and 4-EG to 4-EC were 3.7:1 and 0.7:1, respectively. The feasibility of a cheaper fluorimetric approach to 4-EC quantification was investigated.
Larcher
R
R
IASMA Research Center, Agrifood Quality Department, via Mach 1, 38010 San Michele all'Adige (TN), Italy. roberto.larcher@ismaa.it
Nicolini
G
G
Bertoldi
D
D
Nardin
T
T
D002396:Catechols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D010636:Phenols / Q000032:analysis; D014835:Volatilization; D014867:Water; D014920:Wine / Q000032:analysis*
D002396:Catechols; D010636:Phenols; D014867:Water; C527200:4-ethylcatechol