title: Profiling of environmental Legionella pneumophila strains by randomly amplified polymorphic DNA method isolated from geographically nearby buildings.
abstract: Legionella pneumophila (L. pneumophila) which is also known as etiologic agent Legionnaires Disease lives in natural water and man made water systems. These bacteria belonging to Legionellaceae family are divided 15 serogroups. Phenotypical methods used for the identification of Legionella isolates are not very discriminatory. In this study we investigated genotypic features of eight L. pneumophila serogroup 1 and 18 L. pneumophila serogroup 2-14 strains isolated from different buildings in Istanbul by randomly amplified polymorphic DNA (RAPD) method. Eight L. pneumophila serogroup 1 strains (37.5%) were similar RAPD profile and they were isolated from buildings located in a short distance (about 500 m). Four L. pneumophila serogroup 2-14 strains (22%) were identical genotypically. Three of these strains were isolated from buildings located in a short distance.
authors.affiliation: Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey. zzeybek@yahoo.com
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Zeybek
authors.forename: Irfan
authors.initials: I
authors.lastname: Türetgen
authors.forename: Ayten
authors.initials: A
authors.lastname: Kimiran Erdem
authors.forename: Gönül
authors.initials: G
authors.lastname: Filoğlu
authors.forename: Ayşin
authors.initials: A
authors.lastname: Cotuk
mesh_terms: D001108:Architecture; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics*; D007877:Legionnaires' Disease / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D014881:Water Supply