title: Phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-DCPIP assay.
abstract: Thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using long-chain hydrocarbons (waste car engine oil, base oil or the c-alkane fraction of base oil) as the sole carbon and energy source. A phylogenetic tree of the isolates constructed using their 16S rDNA sequences revealed that the isolates were divided into six genera plus one family (Acinetobacter, Rhodococcus, Gordonia, Pseudomonas, Ralstonia, Bacillus and Alcaligenaceae, respectively). Furthermore, most of the isolates (27 of 36) were classified into the genera Acinetobacter, Rhodococcus or Gordonia. The hydrocarbon-degradation similarity in each strain was confirmed by the 2,6-dichlorophenol indophenol (2,6-DCPIP) assay. Isolates belonging to the genus Acinetobacter degraded long-chain normal alkanes (n-alkanes) but did not degrade short-chain n-alkanes or cyclic alkanes (c-alkanes), while isolates belonging to the genera Rhodococcus and Gordonia degraded both long-chain n-alkanes and c-alkanes.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Nojihigashi 1-1-1, Kusatsu, Shiga, Japan.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Kubota
authors.forename: Daisuke
authors.initials: D
authors.lastname: Koma
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Matsumiya
authors.forename: Seon-Yong
authors.initials: SY
authors.lastname: Chung
authors.forename: Motoki
authors.initials: M
authors.lastname: Kubo
mesh_terms: D015086:2,6-Dichloroindophenol / Q000737:chemistry*; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D006838:Hydrocarbons / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D006838:Hydrocarbons; D015086:2,6-Dichloroindophenol