Analysis of disaccharides and tetrasaccharides released from glycosaminoglycans.
Glycosaminoglycans (GAGs) are converted to disaccharides by various methods. This unit describes separation of individual disaccharides by paper chromatography or paper electrophoresis or high-performance liquid chromatography (HPLC). Lyase-released disaccharides can also be monitored by UV absorbance. Support protocols describe mild conditions for reduction of alkali-labile disaccharides obtained by cleavage of GAGs with lyases, scintillation counting of samples obtained from HPLC separation of radiolabeled saccharides, and calculations for the quantitation of disaccharides.
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University of Illinois, Urbana, Illinois, USA.
D000468:Alkalies; D001894:Borohydrides; D002845:Chromatography; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction
D000468:Alkalies; D001894:Borohydrides; D004187:Disaccharides; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D008190:Lyases