Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR.
We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of approximately 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.
Desai
Prerak T
PT
Utah State University, Center for Integrated BioSystems, 4700 Old Main Hill, Logan, UT 84322-4700, USA.
Walsh
Marie K
MK
Weimer
Bart C
BC
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D005732:Gangliosides / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D005732:Gangliosides