title: Strategies for the synthesis of fluorescently labelled PNA.
abstract: A simple and effective strategy for preparing fluorophore-labelled PNA is described. A C-terminal S-t-butylmercaptocysteine-derivatized PNA was prepared on solid-phase using Fmoc chemistry. Selective deprotection of the S-t-butylmercapto group on-bead, allowed the free thiol to be reacted with a fluorophore derivatized via an iodoacetamido or maleimido linker. Subsequent cleavage and sidechain deprotection yielded C-terminal labelled PNA in good yield and purity. Dual labelled PNA was also prepared by using both C-terminal (-SH) and N-terminal (-NH(2)) labelling chemistries.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield, Cambridge CB2 1EW, UK.
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Liu
authors.forename: Shankar
authors.initials: S
authors.lastname: Balasubramanian