title: Retinoic acid-inducible gene-I is induced by interferon-gamma and regulates CXCL11 expression in HeLa cells.
abstract: Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family proteins, which have diverse roles in the regulation of gene expression and cellular functions. RIG-I is one of the factors regulated by interferon (IFN)-gamma and regarded as an intracellular signaling molecule for IFN-gamma. IFN-gamma is a major cytokine and also suggested to be involved in embryonal implantation and pregnancy. It is demonstrated that IFN-gamma stimulates endometrial epithelial cells to produce CXCL11, which is implicated in implantation. The aim of the present study was to investigate the effect of IFN-gamma on RIG-I expression in HeLa cells, a cell line derived from human uterine carcinoma. We found that RIG-I mRNA and protein were expressed in HeLa cells stimulated with IFN-gamma. The effect of IFN-gamma was observed in concentration- and time-dependent manners. The RNA interference against RIG-I resulted in the suppression of the IFN-gamma-induced CXCL11 expression. Immunohistochemical studies revealed the RIG-I expression in the normal human endometrium, suggesting a possible role of RIG-I in human reproductive organs.
authors.affiliation: Department of Obstetrics and Gynecology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562, Japan. yuzawa@cc.hirosaki-u.ac.jp
authors.forename: Ei
authors.initials: E
authors.lastname: Yuzawa
authors.forename: Tadaatsu
authors.initials: T
authors.lastname: Imaizumi
authors.forename: Tomoh
authors.initials: T
authors.lastname: Matsumiya
authors.forename: Hidemi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Rie
authors.initials: R
authors.lastname: Fukuhara
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Kimura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukui
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tanji
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Mori
authors.forename: Koichi
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Syunsaku
authors.initials: S
authors.lastname: Fujii
authors.forename: Hideki
authors.initials: H
authors.lastname: Mizunuma
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
mesh_terms: D054371:Chemokine CXCL11 / Q000235:genetics / Q000378:metabolism*; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D011247:Pregnancy; D034622:RNA Interference; D011971:Receptors, Immunologic
chemical_list: C515202:CXCL11 protein, human; D054371:Chemokine CXCL11; D011971:Receptors, Immunologic; D007371:Interferon-gamma; C452820:RIGI protein, human; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases