Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector.
Plant innate immunity relies on the recognition of pathogen effector molecules by nucleotide-binding-leucine-rich repeat (NB-LRR) immune receptor families. Previously we have shown the N immune receptor, a member of TIR-NB-LRR family, indirectly recognizes the 50 kDa helicase (p50) domain of Tobacco mosaic virus (TMV) through its TIR domain. We have identified an N receptor-interacting protein, NRIP1, that directly interacts with both N's TIR domain and p50. NRIP1 is a functional rhodanese sulfurtransferase and is required for N to provide complete resistance to TMV. Interestingly, NRIP1 that normally localizes to the chloroplasts is recruited to the cytoplasm and nucleus by the p50 effector. As a consequence, NRIP1 interacts with N only in the presence of the p50 effector. Our findings show that a chloroplastic protein is intimately involved in pathogen recognition. We propose that N's activation requires a prerecognition complex containing the p50 effector and NRIP1.
Caplan
Jeffrey L
JL
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Mamillapalli
Padmavathi
P
Burch-Smith
Tessa M
TM
Czymmek
Kirk
K
Dinesh-Kumar
S P
SP
D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D000956:Antigens, Viral / Q000276:immunology; D002467:Cell Nucleus / Q000737:chemistry; D002736:Chloroplasts / Q000737:chemistry; D003593:Cytoplasm / Q000737:chemistry; D007113:Immunity, Innate; D009687:Nuclear Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D000078723:Nuclear Receptor Interacting Protein 1; D010935:Plant Diseases / Q000276:immunology*; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism; D014026:Nicotiana / Q000276:immunology* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000276:immunology*; D020798:Two-Hybrid System Techniques
D048868:Adaptor Proteins, Signal Transducing; D000956:Antigens, Viral; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D010940:Plant Proteins; D011991:Receptors, Virus; D013884:Thiosulfate Sulfurtransferase