[Role of carbon substrates of culture media on Mycobacterium smegmatis susceptibility to antitubercular agents].
Influence of medium composition on Mycobacterium smegmatis growth and susceptibility to antituberculosis drugs (ATD)--isoniazid and rifabutin--was studied. It was shown that addition of phospholipids (PL) in form of liposomes to meat peptone broth resulted in activation of M. smegmatis growth and decrease of its susceptibility to ATD. Growth characteristics of M. smegmatis and its susceptibility to ATD were studied using variants of modified on source of carbon synthetic medium Sauton as growth substrate. It was revealed that presence of acetate or PL in growth medium results in significant decrease of M. smegmatis susceptibility to isoniazid and rifabutin. It was suggested that this phenomenon is determined by activation of glyoxylate cycle by PL and fatty acids, which, in its turn, can stimulate expression of a number of proteins, including cell membrane pumps excreting antibiotics out of microbial cell.
Shakina
Iu V
IuV
Troshkina
O A
OA
Petrova
E E
EE
Salina
E G
EG
Sorokoumova
G M
GM
Shvets
V I
VI
Kapel'iants
A S
AS
Selishcheva
A A
AA
D000085:Acetates; D000995:Antitubercular Agents / Q000494:pharmacology*; D003470:Culture Media; D005990:Glycerol; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000254:growth & development*; D010743:Phospholipids; D017828:Rifabutin / Q000494:pharmacology*
D000085:Acetates; D000995:Antitubercular Agents; D003470:Culture Media; D010743:Phospholipids; D017828:Rifabutin; D005990:Glycerol; D007538:Isoniazid