Splenomegaly and modified erythropoiesis in KLF13-/- mice.
To study the function of the Krüppel-like transcription factor KLF13 in vivo, we generated mice with a disrupted Klf13 allele. Although Klf13(-/-) mice are viable, fewer mice were present at 3 weeks than predicted by Mendelian inheritance. Viable Klf13(-/-) mice had reduced numbers of circulating erythrocytes and a larger spleen. The spleen contained an increased number of Ter119(med)CD71(hi), Ter119(hi)CD71(hi), and Ter119(hi)CD71(med) cells but not Ter119(hi)CD71(-) cells, indicating an increase in less mature erythroblasts. A higher proportion of the Ter119(med)CD71(hi) cells were proliferating, indicating that the mice were under a degree of erythropoietic stress. These data indicate that KLF13 is involved in the normal control of erythropoiesis.
Gordon
Adele R
AR
Section of Cardiovascular Biology, Department of Biochemistry, University of Cambridge, Downing Site, Cambridge, CB2 1QW, United Kingdom.
Outram
Susan V
SV
Keramatipour
Mohammad
M
Goddard
Catherine A
CA
Colledge
William H
WH
Metcalfe
James C
JC
Hager-Theodorides
Ariadne L
AL
Crompton
Tessa
T
Kemp
Paul R
PR
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D001772:Blood Cell Count; D001853:Bone Marrow / Q000473:pathology; D018797:Cell Cycle Proteins / Q000235:genetics; D002454:Cell Differentiation; D049109:Cell Proliferation; D004900:Erythroblasts / Q000473:pathology; D004920:Erythropoiesis; D017353:Gene Deletion; D005786:Gene Expression Regulation; D018390:Gene Targeting; D005838:Genotype; D051741:Kruppel-Like Transcription Factors / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009929:Organ Size; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D013163:Splenomegaly / Q000473:pathology / Q000503:physiopathology*
D018797:Cell Cycle Proteins; C486422:Klf13 protein, mouse; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D012097:Repressor Proteins