Metagenomic retrieval of a ribosomal DNA repeat array from an uncultured marine alveolate.
The aim of this study was to explore the use of large-scale sequencing to better describe the genome content of naturally occurring, uncultured protists. We constructed a metagenomic fosmid library from a picoplanktonic assemblage (0.2-3 mum size cells) collected at the Blanes Bay Microbial Observatory (Western Mediterranean). Seven clones contained a small-subunit ribosomal RNA gene (SSU rDNA) affiliating with prasinophytes and uncultured alveolates. One clone (FBB25; 35 kb in size) was completely sequenced and found to be a tandem repeat array (5.5 times) of the rDNA operon, including three rRNA genes (SSU, large-subunit and 5.8S rDNAs) and three spacer regions (internal transcribed spacers 1, 2 and intergenic spacer). The SSU rDNA of FBB25 affiliated with the marine alveolates group I, cluster 1, and was almost identical to sequences retrieved only in marine surveys from a wide geographic and ecological range. Phylogenetic trees using the different rRNA genes showed FBB25 as an independent branch among the main alveolate groups, but their closest affiliation varied between the SSU tree (dinoflagellates) and the large-subunit and 5.8S trees (perkinsids). The spacer regions of FBB25 were particularly short when compared with other eukaryotes, indicating a possible genome streamlining in this picoeukaryote. Finally, not a single polymorphism was found in the rDNA repeat array, suggesting that the high SSU rDNA variability typically found in molecular surveys derives from organismal and not intragenomic diversity. This first report on the rDNA genomic structure of an uncultured marine alveolate improves their phylogenetic position and helps interpreting data generated during picoeukaryotic molecular surveys.
Massana
Ramon
R
Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC. Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain. ramonm@icm.csic.es
Karniol
Baruch
B
Pommier
Thomas
T
Bodaker
Idan
I
Béjà
Oded
O
D000818:Animals; D003470:Culture Media; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004141:Dinoflagellida / Q000235:genetics; D056890:Eukaryota / Q000235:genetics* / Q000254:growth & development; D023281:Genomics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012623:Seawater / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences / Q000235:genetics*
D003470:Culture Media; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer