Bioremediation of PCDD/Fs-contaminated municipal solid waste incinerator fly ash by a potent microbial biocatalyst.
Removal of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) from fly ash poses a serious problem. In the study presented here, we used a microbial biocatalyst which is a mixture of 4 bacterial and 5 fungal dioxin-degrading strains. The ability of this biocatalyst to bioremediate PCDD/Fs from contaminated municipal solid waste incinerator (MSWI) fly ash was examined by solid-state fermentation under laboratory conditions. Treatment of MSWI fly ash with the microbial biocatalyst for 21 days resulted in a 68.7% reduction in total toxic PCDD/Fs. Further analyses revealed that the microbial biocatalyst also removed 66.8% of the 2,3,7,8-substituted congeners from the fly ash. During the treatment period, the presence of the individual strains composing the microbial biocatalyst was monitored by the amplification of strain-specific DNA sequences followed by denaturing gradient gel electrophoresis (DGGE). This analysis showed that all of the bacterial and fungal strains composing this dioxin-degrading microbial mixture maintained under the dioxin treatment conditions. These results demonstrate that this microbial biocatalyst could potentially be used in the bioremediation of PCDD/Fs from contaminated fly ash.
Nam
In-Hyun
IH
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.
Kim
Young-Mo
YM
Murugesan
Kumarasamy
K
Jeon
Jong-Rok
JR
Chang
Yoon-Young
YY
Chang
Yoon-Seok
YS
D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D001572:Benzofurans / Q000032:analysis*; D001673:Biodegradation, Environmental; D002244:Carbon / Q000737:chemistry*; D002947:Cities; D060729:Coal Ash; D000072338:Dibenzofurans, Polychlorinated; D005658:Fungi / Q000254:growth & development / Q000302:isolation & purification; D006256:Hazardous Waste / Q000032:analysis*; D017745:Incineration; D052638:Particulate Matter / Q000737:chemistry*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis
D001572:Benzofurans; D060729:Coal Ash; D000072338:Dibenzofurans, Polychlorinated; D006256:Hazardous Waste; D052638:Particulate Matter; D000072317:Polychlorinated Dibenzodioxins; D002244:Carbon