title: Mouse embryonic stem (ES) cell isolation.
abstract: Building on the technology presented in earlier units of Chapter 23, this unit describes the basic method for the isolation of mouse embryonic (ES) stem cells. ES cells from wild-type mice may be used for the production of mouse mutants by homologous recombination and blastocyst-mediated transgenesis. ES cells from mutant mouse lines may be used in the analysis of mutant phenotypes.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Conner
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D005780:Gelatin; D007202:Indicators and Reagents; D051379:Mice; D013234:Stem Cells / Q000166:cytology*
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D005780:Gelatin