Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1.
Brucella is an intracellular pathogen able to persist for long periods of time within the host and establish a chronic disease. We show that soon after Brucella inoculation in intestinal loops, dendritic cells from ileal Peyer's patches become infected and constitute a cell target for this pathogen. In vitro, we found that Brucella replicates within dendritic cells and hinders their functional activation. In addition, we identified a new Brucella protein Btp1, which down-modulates maturation of infected dendritic cells by interfering with the TLR2 signaling pathway. These results show that intracellular Brucella is able to control dendritic cell function, which may have important consequences in the development of chronic brucellosis.
Salcedo
Suzana P
SP
Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Faculté de Sciences de Luminy, Marseille, France.
Marchesini
María Ines
MI
Lelouard
Hugues
H
Fugier
Emilie
E
Jolly
Gilles
G
Balor
Stephanie
S
Muller
Alexandre
A
Lapaque
Nicolas
N
Demaria
Olivier
O
Alexopoulou
Lena
L
Comerci
Diego J
DJ
Ugalde
Rodolfo A
RA
Pierre
Philippe
P
Gorvel
Jean-Pierre
JP
D000818:Animals; D002003:Brucella abortus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D004195:Disease Models, Animal; D015536:Down-Regulation; D054884:Host-Pathogen Interactions; D007082:Ileum / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000382:microbiology; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
D016207:Cytokines; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2