Expression and regulation of periostin/OSF-2 gene in rat uterus and human endometrium.
Periostin/OSF2 is a ligand for alphavbeta3 and alphavbeta5 integrins and activates the Akt/PKB pathway. Recent reports of periostin/OSF2 gene disrupted mice indicate that periostin/OSF-2 plays an important role in implantation. Quantitative RT-PCR revealed that the expression of periostin/OSF-2 mRNA in rat uteri was reduced to approximately 10% at 12 h after 17beta-estradiol (E2) injection, but was not changed after progesterone (P) injection. RT-PCR revealed the expression of periostin/OSF-2 in human endometrium, cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells. In ESCs, the expression of periostin/OSF-2 mRNA was reduced to approximately 50% at 6 h after E2 treatment. The amount of periostin/OSF2 mRNA in human endometrium significantly increased during mid-proliferative and early secretory phases of menstrual cycle, and decreased during late-proliferative, mid-secretory and late secretory phases. The expression of periostin/OSF2 mRNA significantly decreased in ESCs decidualized by treatment with E2 and P for 7 and 11 days. By immunohistochemistry, the expression of periostin/OSF-2 was strongly detected in endometrial stromal cells during early proliferative, mid-proliferative and early secretory phases, and was strongly detected in endometrial epithelial cells during late secretory phase. This study demonstrated that the expression of periostin/OSF-2 is regulated by ovarian steroid hormones in rat uterus and human endometrium.
Hiroi
Hisahiko
H
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Hongo, Japan.
Momoeda
Mikio
M
Nakazawa
Fumiko
F
Koizumi
Minako
M
Tsutsumi
Ryo
R
Hosokawa
Yumi
Y
Osuga
Yutaka
Y
Yano
Tetsu
T
Tsutsumi
Osamu
O
Taketani
Yuji
Y
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008597:Menstrual Cycle / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism*
D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D012333:RNA, Messenger; D011374:Progesterone