Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease.
This study determined the effects of pcDNA3-beta-nerve growth factor (NGF) gene-modified bone marrow stromal cells (BMSC) on the rat model of Parkinson's disease (PD). The recombinant plasmid pcDNA3-beta-NGF was transfected into BMSC, and NGF expression and its biological activity in vitro were detected. BMSC modified by the NGF gene were then grafted into the corpus striatum of PD rats, and the rotation behavior was evaluated at 1, 2, 4, and 6 weeks post-transplantation. A significant improvement in rotation behavior was observed in PD rats subjected to cell transplantation, especially in PD rats receiving NGF-modified BMSC. The genetically modified BMSC survived and expressed beta-NGF but did not differentiate into tyrosine hydroxylase-positive cells in vivo. The present findings suggested that genetically modified BMSC could be effective for PD treatment, and the mechanisms might involve the neuroprotective effects of beta-NGF.
Wang
Ting-Hua
TH
Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China. tinghua_neuron@263.net
Feng
Zhong-Tang
ZT
Wei
Peng
P
Li
Hui
H
Shi
Zhen-Jiang
ZJ
Li
Li-Yan
LY
D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D003342:Corpus Striatum / Q000166:cytology / Q000502:physiology / Q000601:surgery; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D006085:Graft Survival; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009043:Motor Activity; D020932:Nerve Growth Factor / Q000235:genetics*; D020734:Parkinsonian Disorders / Q000235:genetics* / Q000628:therapy*; D010957:Plasmids; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000235:genetics; D017154:Stromal Cells / Q000637:transplantation*; D014162:Transfection
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D020932:Nerve Growth Factor