title: [Studies on quality standard of Yifu ointment].
abstract: OBJECTIVE
  To develop a method for the determination of Chonglou saponin I and Chonglou saponin II in Yifu ointment.
  
  
  METHODS
  The chromatographic separation was performed on Hypersil ODS2 C18 column (4.6 mm x 150 mm, 5 microm) and IBBM-612111 ODS C18 guard column. Acetonitrile-0.02% phosphoric (43:57) as mobile phase. The flow rate was 1 mL min(-1) and column temperature was set at 40 degrees C. The UV detection wavelength was set 203 nm.
  
  
  RESULTS
  Chonglou saponin I showed a good linear relationship at a range of 0.1024-3.2 microg, r =0.9998, the average recovery was 97.4%, and RSD was 1.8% (n = 6); Chonglou saponin II showed a good linear relationship at a range of 0.064-2.0 microg, r = 0.9999, the average recovery was 101.4%, and RSD was 1.0% (n =6).
  
  
  CONCLUSIONS
  The method is accurate with the good reproducibility and can be used for the quality control of Yifu Ointment.
authors.affiliation: Ethnomidicine Institute of Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
authors.forename: You-ming
authors.initials: YM
authors.lastname: Pu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Gu
authors.forename: Xian-rong
authors.initials: XR
authors.lastname: Lai
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Ren-chao
authors.initials: RC
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D019668:Liliaceae / Q000737:chemistry; D009824:Ointments; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D027824:Rosaceae / Q000737:chemistry; D012503:Saponins / Q000032:analysis*
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D009824:Ointments; D012503:Saponins