Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection.
Chronic active Epstein-Barr virus infection (CAEBV) is characterized by recurrent infectious mononucleosis-like symptoms and has high mortality and morbidity. To clarify the mechanisms of CAEBV, the gene-expression profiles of peripheral blood obtained from patients with CAEBV were investigated. Twenty genes were differentially expressed in 4 patients with CAEBV. This microarray result was verified using a real-time reverse-transcriptase polymerase chain reaction assay in a larger group of patients with CAEBV. Eventually, 3 genes were found to be significantly upregulated: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5. These genes may be associated with the inflammatory reaction or with cell proliferation.
Ito
Yoshinori
Y
Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Shibata-Watanabe
Yukiko
Y
Ushijima
Yoko
Y
Kawada
Jun-Ichi
J
Nishiyama
Yukihiro
Y
Kojima
Seiji
S
Kimura
Hiroshi
H
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D019204:GTP-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007244:Infectious Mononucleosis / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; C490287:GBP1 protein, human; C528873:GBP5 protein, human; C072273:TNFAIP6 protein, human; D019204:GTP-Binding Proteins