Prophages in Listeria monocytogenes contain single-nucleotide polymorphisms that differentiate outbreak clones within epidemic clones.
A fragment end ligation-mediated PCR strategy was used to analyze the AscI pulsed-field gel electrophoresis patterns of Listeria monocytogenes epidemic clone II (ECII), which led to the identification of single-nucleotide polymorphisms (SNPs) in prophage regions that differentiated the two ECII outbreak clones. SNPs in prophages that differentiated the outbreak clones of ECIII and -IV were also identified.
Chen
Yi
Y
Center for Food Safety and Applied Nutrition, FDA, HFS-712, 5100 Paint Branch Parkway, College Park, MD 20740, USA. yi.chen@fda.hhs.gov
Knabel
Stephen J
SJ
D015373:Bacterial Typing Techniques; D004196:Disease Outbreaks; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000821:virology; D008088:Listeriosis / Q000453:epidemiology* / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide; D039002:Prophages / Q000235:genetics*; D014774:Virulence