Methods for selection of aptamers to protein targets.
Aptamers are synthetic single-stranded RNA or DNA molecules capable of specific binding to other target molecules. In this review, the main aptamer properties are considered and methods for selection of aptamers against various protein targets are described. Special attention is given to the methods for directed selection of aptamers, which allow one to obtain ligands with specified properties.
Kulbachinskiy
A V
AV
Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, 123182 Moscow, Russia. akulb@img.ras.ru
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry*; D000939:Epitopes; D015723:Gene Library; D005821:Genetic Techniques; D008024:Ligands; D009841:Oligonucleotides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D052156:SELEX Aptamer Technique / Q000379:methods*; D012641:Selection, Genetic
D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D000939:Epitopes; D008024:Ligands; D009841:Oligonucleotides; D011506:Proteins; D012313:RNA