RNA interference in Caenorhabditis elegans.
RNAi has become an essential tool in C. elegans research. Procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA and soaking in dsRNA solution are described in this unit.
Conte
Darryl
D
University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Mello
Craig C
CC
D000284:Administration, Oral; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D003433:Crosses, Genetic; D012734:Disorders of Sex Development; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics; D007202:Indicators and Reagents; D008297:Male; D008845:Microinjections; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000235:genetics; D018510:RNA, Helminth / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D012869:Skin Absorption
D029742:Caenorhabditis elegans Proteins; D018398:Homeodomain Proteins; D007202:Indicators and Reagents; D012330:RNA, Double-Stranded; D018510:RNA, Helminth; D034741:RNA, Small Interfering; C122185:rde-1 protein, C elegans; C119682:unc-42 protein, C elegans