Herpesvirus saimiri transformation may help disclose inherent functional defects of mucosal T lymphocytes in patients with gastric adenocarcinoma.
To dissect the phenotypic and functional features of mucosal T lymphocytes in patients with gastric adenocarcinoma, we have used the Herpesvirus saimiri transformation procedure to achieve T-cell lines from gastric origin. Once achieved, cell function was assessed by in vitro stimulation with mitogens. CD2-specific monoclonal antibodies (alpha-CD2), alone or in combination with interleukin (IL)-2, rendered fewer counts in patients (34 408+/-3965 and 52 157+/-6473 c.p.m., respectively) than in controls (67 471+/-11 755 c.p.m., P<0.01 and 77 864+/-12 545 c.p.m., P<0.05, respectively). Likewise, CD3-based responses were defective in cancer cell lines: alpha-CD3 (54 794+/-9269 vs 86 104+/-10 341 c.p.m., P<0.01), alpha-CD3+IL-2 (57 789+/-8590 vs 88855+/-8516 c.p.m., P<0.01) and alpha-CD3+alpha-CD2 (52 130+/-7559 vs 120 852+/-16 552 c.p.m., P<0.01). Finally, IL-2 failed to adequately stimulate patient cell lines (39 310+/-4023 vs 60 945+/-9463 c.p.m., P<0.05). These results suggest that mucosal T lymphocytes in cancer patients are inherently impaired in their proliferative ability. This may be crucial in the control of tumour growth.
Valeri
Anna P
AP
Inmunología, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Aguilera-Montilla
Noemí
N
López-Santalla
Mercedes
M
Mencía
Angeles
A
Rodríguez-Juan
Cristina
C
Gutiérrez-Calvo
Alberto
A
Martín
Javer
J
Lasa
Inmaculada
I
García-Sancho
Luis
L
Granell
Javier
J
Pérez-Blas
Mercedes
M
Martín-Villa
José M
JM
D000230:Adenocarcinoma / Q000276:immunology* / Q000473:pathology; D000368:Aged; D015703:Antigens, CD / Q000276:immunology; D002461:Cell Line, Transformed; D049109:Cell Proliferation; D002472:Cell Transformation, Viral; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000473:pathology*; D006567:Herpesvirus 2, Saimiriine; D006801:Humans; D007111:Immunity, Cellular; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology* / Q000821:virology
D015703:Antigens, CD