title: Spatial interplay between PIASy and FIP200 in the regulation of signal transduction and transcriptional activity.
abstract: The members of the protein inhibitor of activated STAT (PIAS) family of proteins are implicated in fundamental cellular processes, including transcriptional regulation, either through action as E3 SUMO ligases or through SUMO-independent effects. We report here the identification of FIP200 (focal adhesion kinase family-interacting protein of 200 kDa) as a new PIASy-interacting protein. We show that the interaction depends on the integrity of the RING finger of PIASy and the carboxy terminus of FIP200. Both in vitro and in vivo sumoylation assays failed to reveal any sumoylation of FIP200, suggesting that FIP200 is not a bona fide SUMO substrate. Immunofluorescence microscopy and subcellular fractionation, either upon forced PIASy expression or in the absence of PIASy, revealed that interaction with PIASy redistributes FIP200 from the cytoplasm to the nucleus, correlating with abrogation of FIP200 regulation of TSC/S6K signaling. Conversely, FIP200 enhances the transcriptional activation of the p21 promoter by PIASy whereas PIASy transcription activity is severely reduced upon FIP200 depletion by RNA interference. Chromatin immunoprecipitation analysis demonstrates that endogenous PIASy and FIP200 are corecruited to the p21 promoter. Altogether, these results provide the first evidence for the existence of a close-spatially controlled-mode of regulation of FIP200 and PIASy nucleocytoplasmic functions.
authors.affiliation: Nuclear Organization and Oncogenesis Unit, INSERM U579, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Martin
authors.forename: Klaus
authors.initials: K
authors.lastname: Schwamborn
authors.forename: Henning
authors.initials: H
authors.lastname: Urlaub
authors.forename: Boyi
authors.initials: B
authors.lastname: Gan
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: Guan
authors.forename: Anne
authors.initials: A
authors.lastname: Dejean
mesh_terms: D000818:Animals; D000071183:Autophagy-Related Proteins; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D000075223:Poly-ADP-Ribose Binding Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000378:metabolism; D015398:Signal Transduction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D000071183:Autophagy-Related Proteins; D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C485891:PIAS4 protein, human; C414349:Piasy protein, mouse; D000075223:Poly-ADP-Ribose Binding Proteins; D050828:Protein Inhibitors of Activated STAT; C407704:RB1CC1 protein, human; C428743:Rb1cc1 protein, mouse; D025841:Small Ubiquitin-Related Modifier Proteins; C486009:TESC protein, human; D011505:Protein-Tyrosine Kinases; D038762:Ribosomal Protein S6 Kinases, 70-kDa