Retrovirus infection of cells in vitro and in vivo.
There are many applications in which retrovirus vectors are used as transduction agents. In some cases, the vector carries a gene that one wishes to express in a target cell in order to study the function of that gene. In other cases, the virus is used to introduce a histochemical marker gene into cells in order to follow their fate. Retrovirus vectors can also be used in a variety of cells type to investigate regulatory sequences in which a reporter gene and regulatory sequences are carried by the vector and to immortalize or transform primary cells by transduction of oncogenes. For each application, the infection protocol may vary and must often be optimized. Guidelines for infection of cells in some typical in vivo and in vitro experiments are presented in this overview.
Cepko
C
C
Harvard Medical School, Boston, Massachusetts, USA.
Pear
W
W
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D015723:Gene Library; D017930:Genes, Reporter; D005822:Genetic Vectors; D007668:Kidney; D051379:Mice; D011247:Pregnancy; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D012192:Retroviridae Infections / Q000196:embryology / Q000635:transmission; D012377:Rodentia; D014161:Transduction, Genetic / Q000379:methods
D018076:DNA, Complementary; D004279:DNA, Viral