Unnatural translation initiation.
Protein translation in nature always begins with an initiator transfer RNA (tRNA) carrying the amino acid methionine. This was circumvented in vitro with a reconstituted translation system utilizing initiator tRNA synthetically mischarged with the other natural amino acids. In addition, it was determined that this system could accommodate these non-methionine amino acids containing various N-alpha-acyl groups, many of which are useful for post-translational modification such as peptide cyclization.
Tomsho
John W
JW
The Pennsylvania State University, Department of Chemistry, 414 Wartik Laboratory, University Park, Pennsylvania 16802, USA.
Benkovic
Stephen J
SJ
D049148:Aminoacylation; D018387:Codon, Initiator; D010442:Peptide Chain Initiation, Translational; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry* / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000737:chemistry / Q000378:metabolism*; D046249:Transfer RNA Aminoacylation
D018387:Codon, Initiator; C509376:G7-18NATE; D010456:Peptides, Cyclic; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met