[Determination of seven biogenic amines in fish using micellar electrokinetic capillary chromatography].
A method for the determination of histamine, 2-phenylethylamine, tryptamine, putrescine, cadaverine, spermidine and spermine in fish using micellar electrokinetic capillary chromatography (MECC) was developed. Seven biogenic amines (BAs) were extracted from a sample with 6% perchloric acid, then derivatized with benzoyl chloride. 0.02 mol/L borate buffer (pH 9.2) containing 0.06 mol/L sodium deoxycholate-methanol (95 : 5, v/v) was used as a running buffer. The detection wavelength was set at 214 nm. The separation was achieved within 12 min. Good linearities were observed from the calibration plots in the concentration ranges. The detection limit of histamine was 15 microg/g and those of the other amines were 5 microg/g. The developed method was successfully employed for the simultaneous determination of 7 BAs in fish sample with satisfactory results.
Gan
Ning
N
Faculty of Material Science and Engineering, Ningbo University, Ningbo 315211, China. ganning@nbu.edu.cn
Li
Tianhua
T
Wang
Luyan
L
Jiang
Qianli
Q
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005399:Fishes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015203:Reproducibility of Results
D001679:Biogenic Amines