[Larrad biliopancreatic diversion. Description of an rat experimental model].
OBJECTIVE.: To validate the experimental model of Larrad-biliopancreatic diversion (LBPD) and to analyze weight gain and mortality in rats fed with non- supplemented diets.


METHODS
Control (6) and experimental (10) male Wistar rats were used. The experimental group was operated on using the human LBPD adapted for rats: Subcardial gastrectomy, a short biliopancreatic channel created at 5 cm from Treitz angle and common channel at 5 cm from ileocecal valve. After surgery recovery (7 days) the rats were fed ab libitum with a standard non-supplemented diet (without proteins, minerals or vitamins). Percentage of weight lost or gained up to the end of the experiment was analyzed.


RESULTS
The control animals gained weight progressively from 13.1 +/- 2.4% at day 7 to 58 +/- 9.2% at day 63, when the animals were sacrificed. After LBPD, mortality was 50% at day 25 +/- 17.5(range, 14-56), no significant differences in the percentage of weight lost being found between surviving (-38.9 +/- 14.2%) and non-surviving rats (-29 +/- 5.6%; p = 0.192). Of the surviving animals, 80% progressively lost weight reaching a maximum loss between day 63 (-42.3 +/- 8%) and 70 (-44.1 +/- 9.7%), and 20% lost weight until day 35 and gained over 7% of body weight until sacrifice (day 147).


CONCLUSIONS
An experimental model of LBPD in rats is technically feasible. Both mortality and percentage weight loss are not directly related. The bowel adaptation mechanism could mediate the percentage of weight regain in operated rats.
Larrad Jiménez
Alvaro
A
Servicio de Cirugía General y Digestiva. Unidad de Cirugía Endocrinometabólica. Hospital Quirón. Madrid. España. LARRAD@inicia.es
Alvarez
María Pilar
MP
Fernández
María Pilar
MP
Cano
Pilar
P
Jiménez Ortega
Vanesa
V
Esquifino
Ana Isabel
AI
D000818:Animals; D050110:Bariatric Surgery; D015904:Biliopancreatic Diversion; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D015430:Weight Gain; D015431:Weight Loss