Secondary DNA structure formation for Hoxb9 promoter and identification of its specific binding protein.
Hox genes determine anterior-posterior specificity of an animal body. In mammals, these genes map onto four chromosomal loci in a clustered manner, and their expression is regulated in a coordinated manner according to their chromosomal structure. In the present study, we analysed the Hoxb9 promoter and found that promoter activity in cultured cells is linked to secondary structure formation of promoter DNA. In nuclear extracts, we also detected binding activity specific for secondary-structured DNA. We successfully isolated a candidate gene encoding this specific DNA-binding protein, FBXL10, and demonstrated the effects of the gene product on Hoxb9 promoter activity. Our results suggest that DNA can regulate gene expression by other, non-sequence-specific modes of genetic coding.
Yamagishi
Takumi
T
Kondo Research Unit, Brain Development Research Group, Brain Science Institute, Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Hirose
Shigehisa
S
Kondo
Takashi
T
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D044783:F-Box Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases; D051379:Mice; D009690:Nucleic Acid Conformation; D010089:Oxidoreductases, N-Demethylating / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000502:physiology; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D034622:RNA Interference
D004268:DNA-Binding Proteins; D044783:F-Box Proteins; C491257:HOXB9 protein, human; D018398:Homeodomain Proteins; C104161:Hoxb9 protein, mouse; D004247:DNA; D056484:Jumonji Domain-Containing Histone Demethylases; C534524:KDM2A protein, human; C524747:Kdm2b protein, mouse; D010089:Oxidoreductases, N-Demethylating