Bulky DNA adducts, 4-aminobiphenyl-haemoglobin adducts and diet in the European Prospective Investigation into Cancer and Nutrition (EPIC) prospective study.
In contrast to some extensively examined food mutagens, for example, aflatoxins, N-nitrosamines and heterocyclic amines, some other food contaminants, in particular polycyclic aromatic hydrocarbons (PAH) and other aromatic compounds, have received less attention. Therefore, exploring the relationships between dietary habits and the levels of biomarkers related to exposure to aromatic compounds is highly relevant. We have investigated in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort the association between dietary items (food groups and nutrients) and aromatic DNA adducts and 4-aminobiphenyl-Hb adducts. Both types of adducts are biomarkers of carcinogen exposure and possibly of cancer risk, and were measured, respectively, in leucocytes and erythrocytes of 1086 (DNA adducts) and 190 (Hb adducts) non-smokers. An inverse, statistically significant, association has been found between DNA adduct levels and dietary fibre intake (P = 0.02), vitamin E (P = 0.04) and alcohol (P = 0.03) but not with other nutrients or food groups. Also, an inverse association between fibre and fruit intake, and BMI and 4-aminobiphenyl-Hb adducts (P = 0.03, 0.04, and 0.03 respectively) was observed. After multivariate regression analysis these inverse correlations remained statistically significant, except for the correlation adducts v. fruit intake. The present study suggests that fibre intake in the usual range can modify the level of DNA or Hb aromatic adducts, but such role seems to be quantitatively modest. Fibres could reduce the formation of DNA adducts in different manners, by diluting potential food mutagens and carcinogens in the gastrointestinal tract, by speeding their transit through the colon and by binding carcinogenic substances.
Peluso
Marco
M
Cancer Risk Factor Branch, Analytical and Biomolecular Cytology Unit, CSPO-Scientific Institute of Tuscany, Florence, Italy.
Airoldi
Luisa
L
Munnia
Armelle
A
Colombi
Alessandro
A
Veglia
Fabrizio
F
Autrup
Herman
H
Dunning
Alison
A
Garte
Seymour
S
Gormally
Emmanuelle
E
Malaveille
Christian
C
Matullo
Giuseppe
G
Overvad
Kim
K
Raaschou-Nielsen
Ole
O
Clavel-Chapelon
Francoise
F
Linseisen
Jacob
J
Boeing
Heiner
H
Trichopoulou
Antonia
A
Palli
Domenico
D
Krogh
Vittorio
V
Tumino
Rosario
R
Panico
Salvatore
S
Bueno-De-Mesquita
Bas H
BH
Peeters
Petra H
PH
Kumle
Merethe
M
Agudo
Antonio
A
Martinez
Carmen
C
Dorronsoro
Miren
M
Barricarte
Aurelio
A
Tormo
Marìa Jose
MJ
Quiros
José Ramón
JR
Berglund
Goran
G
Jarvholm
Bengt
B
Day
Nicholas E
NE
Key
Timothy J
TJ
Saracci
Rodolfo
R
Kaaks
Rudolf
R
Riboli
Elio
E
Bingham
Shelia
S
Vineis
Paolo
P
D000368:Aged; D000393:Air Pollutants / Q000633:toxicity; D000428:Alcohol Drinking; D015415:Biomarkers / Q000032:analysis; D015992:Body Mass Index; D002273:Carcinogens / Q000032:analysis* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000517:prevention & control; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D004043:Dietary Fiber / Q000008:administration & dosage*; D004912:Erythrocytes / Q000737:chemistry*; D005060:Europe; D007887:Fabaceae; D005260:Female; D005638:Fruit; D006454:Hemoglobins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009747:Nutritional Physiological Phenomena; D010126:Ozone / Q000633:toxicity; D011446:Prospective Studies; D014675:Vegetables
D000393:Air Pollutants; D015415:Biomarkers; D002273:Carcinogens; D018736:DNA Adducts; D004043:Dietary Fiber; D006454:Hemoglobins; C062091:hemoglobin, 4-aminobiphenyl-; D010126:Ozone