[Bactericidal activity of GLL-37, a novel derivative of the human antimicrobial peptide LL-37].
To develop and investigate GLL-37, a substitution analogue of the human antimicrobial peptide LL-37 with anti-enzymatic degradation activity and improved efficacy.



The bactericidal activities of LL-37 and newly developed GLL-37 against 6 Gram-negative and -positive bacteria were determined by Broth microdilution assays. The minimum inhibitory concentrations of LL-37 and GLL-37 against E.coli ATCC 25922 in different NaCl concentration medium were also detected. Both peptides were co-incubated with elastase, and then analyzed by PAGE electrophoresis and bactericidal activity determination.



GLL-37 showed a stronger elastase resistance ability than LL-37, and was significantly more effective than LL-37 under high-salt condition.



The antimicrobial peptide GLL-37 derived form LL-37 has the potential as a new therapeutic agent for bacterial infections.
Shi
Lin
L
Department of Immunology and Microbiology, Xioan Jiaotong University School of Medinice, Xioan 710061, China.
Liu
Shan
S
Fan
Gui-xiang
GX
Yuan
Yu-kang
YK
Mei
Long
L
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology* / Q000627:therapeutic use; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D054804:Cathelicidins; D002463:Cell Membrane Permeability / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects; D011552:Pseudomonas Infections / Q000188:drug therapy
D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D008565:Membrane Proteins