title: Caspase-3 activation and DNA damage in pig skin organ culture after solar irradiation.
abstract: In the present study, a convenient and easy-to-handle skin organ culture was developed from domestic pig ears using polycarbonate Transwell culture inserts in 12-well plate. This alternative model was then tested for its suitability in analyzing the short-term effects of a single solar radiation dose (from 55 to 275 kJ.m(-2)). Differentiation of the pig skin was maintained for up to 48 h in culture, and its morphology was similar to that of fresh human skin. Solar irradiation induced a significant release of the cytosolic enzymes lactate dehydrogenase and extracellular signal-related kinase 2 protein in the culture medium 24 h after exposure. These photocytotoxic effects were associated with the formation of sunburn cells, thymine dimers and DNA strand breaks in both the epidermis and dermis. Interestingly, cell death was dose dependent and associated with p53 protein upregulation and strong caspase-3 activation in the basal epidermis. None of these cellular responses was observed in non-irradiated skin. Finally, topical application of a broad-spectrum UVB + A sunfilter formulation afforded efficient photoprotection in irradiated explants. Thus, the ex vivo pig ear skin culture may be a useful tool in the assessment of solar radiation-induced DNA damage and apoptosis, and for evaluating the efficacy of sunscreen formulations.
authors.affiliation: Laboratoire de Pharmacocinétique Cutanée, Institut de Recherche Pierre Fabre, Vigoulet-Auzil, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bacqueville
authors.forename: Alain
authors.initials: A
authors.lastname: Mavon
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D053148:Caspase 3 / Q000378:metabolism* / Q000528:radiation effects*; D002454:Cell Differentiation / Q000528:radiation effects; D004249:DNA Damage; D004789:Enzyme Activation / Q000528:radiation effects; D006801:Humans; D007150:Immunohistochemistry; D009924:Organ Culture Techniques; D012867:Skin / Q000166:cytology / Q000473:pathology / Q000528:radiation effects*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014466:Ultraviolet Rays
chemical_list: D053148:Caspase 3