Synthesis and assessment of fertility-regulating potential of 2-(2''-chloroacetamidobenzyl)-3-(3'-indolyl) quinoline in adult rats as a male contraceptive agent.
BACKGROUND
The purpose of this study was to investigate the fertility-regulating potential of the compound 2-(2''-chloroacetamidobenzyl)-3-(3'-indolyl) quinoline in male rats.


METHODS
Rats of proven fertility were treated with the compound by oral gavage for 1 to 8 consecutive weeks. Functional fertility, testicular, epididymal and seminal vesicular weight, epididymal sperm count and spermatogenesis were quantitated. Reproductive hormones and some biochemical parameters were measured.


RESULTS
Functional fertility was reduced significantly as revealed by a fall in fertility and pregnancy rate. The weight of the reproductive organs was reduced significantly. A reduction of sperm count and number of different types of testicular cells was observed. The treatment with the compound resulted in decline of testosterone and an increase of FSH hormone levels. The compound effectively reduced testicular protein, glycogen and epididymal glyceryl phosphorylcholine. Increase in testicular alkaline phosphatase and cholesterol was also observed. Fertility and other effects were regained gradually after cessation of treatment.


CONCLUSIONS
The results revealed from the study indicate that the compound has reversible antifertility activity and can be explored as male contraceptive agent.
Bhowal
Sujit K
SK
Department of Reproductive Biology Research, Indian Institute of Chemical Biology 4, Jadavpur, Kolkata 700 032, India.
Lala
Sanchita
S
Hazra
Abhijit
A
Paira
Priyankar
P
Banerjee
Sukdeb
S
Mondal
Nirup B
NB
Chakraborty
Smritinath
S
D000284:Administration, Oral; D000818:Animals; D003272:Contraceptive Agents, Male / Q000138:chemical synthesis* / Q000494:pharmacology*; D004822:Epididymis / Q000033:anatomy & histology; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007211:Indoles / Q000138:chemical synthesis* / Q000494:pharmacology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D018873:Pregnancy Rate; D011804:Quinolines / Q000138:chemical synthesis* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012669:Seminal Vesicles / Q000033:anatomy & histology; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism
C443758:2-(2''-dichloroacetamidobenzyl)-3-(3'-indolylquinoline); D003272:Contraceptive Agents, Male; D007211:Indoles; D011804:Quinolines