title: A nonradioisotope, enzymatic microplate assay for in vivo evaluation of 2-deoxyglucose uptake in muscle tissue.
abstract: To determine 2-deoxy-D-glucose (2DG) and 2-deoxy-D-glucose 6-phosphate (DG6P) in mouse tissue after injection of 2DG, we have developed a novel assay. This assay is a simple procedure involving incubation of samples with four independent, single reaction mixtures followed by measurement of fluorescence. From differences between the values obtained with the four reactions, each of glucose, glucose 6-phosphate, 2DG and DG6P were able to be quantified in a sensitive manner. Using this assay system, glucose and 2DG in blood and DG6P-accumulation in muscle were easily determined. Therefore, this assay may be useful for measuring in vivo glucose uptake without the use of radioisotopes.
authors.affiliation: Research Section, Research and Development Department, House Wellness Foods Corporation, 3-20 Imoji, Itami, Hyogo 664-0011, Japan. yamamoto_norio@house-wf.co.jp
authors.forename: Norio
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Kengo
authors.initials: K
authors.lastname: Kawasaki
authors.forename: Takuya
authors.initials: T
authors.lastname: Sato
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hirose
authors.forename: Koutarou
authors.initials: K
authors.lastname: Muroyama
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003847:Deoxyglucose / Q000097:blood / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives / Q000378:metabolism; D008297:Male; D051379:Mice; D032342:Muscle Cells / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001786:Blood Glucose; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate; D003847:Deoxyglucose