Preparation of mouse feeder cells for fusion and cloning.
To maximize the yield of hybrids from fusion and cloning procedures, feeder cells are required to be cocultured with the hybrids, while hybrid cell density is low. Mouse peritoneal cells, most of which are macrophages, have been found to be convenient and effective feeder cells which are a source of soluble growth factors for hybridoma cells.
Fuller
S A
SA
Allelix Inc., Mississauga, Ontario.
Takahashi
M
M
Hurrell
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification*; D018920:Coculture Techniques; D006825:Hybridomas / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal