Guanidine methods for total RNA preparation.
Three different methods for RNA preparation using guanidine are presented in this unit--a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.
Kingston
R E
RE
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Chomczynski
P
P
Sacchi
N
N
D000818:Animals; D002122:Calcium Chloride; D002478:Cells, Cultured / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification; D019791:Guanidine; D007202:Indicators and Reagents; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification*; D012996:Solutions
D007202:Indicators and Reagents; D011506:Proteins; D012996:Solutions; D012313:RNA; D004247:DNA; D019791:Guanidine; D002122:Calcium Chloride