The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells.
In this study we present the enzymatic and biological analysis of polysialic acid (polySia) based hydrogel in terms of its degradation and cytocompatibility. PolySia based hydrogel is completely degradable by endosialidase enzyme which may avoid second surgery after tissue recovery. Viability assay showed that soluble components of polySia hydrogel did not cause any toxic effect on cultured Schwann cells. Moreover, green fluorescence protein transfected neonatal and adult Schwann cells, neural stem cells and dorsal root ganglionic cells (unlabelled) were seeded on polySia hydrogel modified with poly-L-lysine (Pll), poly-L-ornithine-laminin (porn-laminin) or collagen. Water soluble tetrazolium salt assay revealed that modification of the hydrogel significantly improved cell adhesion and viability. These results infer that polySia based scaffolds in combination with cell adhesion molecules and cells genetically modified to express growth factors would potentially be promising alternative in reconstructive therapeutic strategies.
Haile
Yohannes
Y
Hannover Medical School, Institute of Neuroanatomy, OE-4140, Carl-Neuberg-Strasse 1, Hannover, Germany.
Berski
Silke
S
Dräger
Gerald
G
Nobre
Andrè
A
Stummeyer
Katharina
K
Gerardy-Schahn
Rita
R
Grothe
Claudia
C
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020100:Hydrogels / Q000008:administration & dosage* / Q000737:chemistry; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012794:Sialic Acids / Q000008:administration & dosage* / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D020100:Hydrogels; D012794:Sialic Acids; C021319:polysialic acid