title: Yeast Polo-like kinase substrates are nailed with the right tools.
abstract: A platform has been built combining chemical genetics and bioinformatics to screen the proteome for physiological substrates of the Polo-like kinase in budding yeast. A novel role for this kinase in regulating the mitotic spindle is revealed.
authors.affiliation: Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK. va228@cam.ac.uk
authors.forename: Vincent
authors.initials: V
authors.lastname: Archambault
authors.forename: David M
authors.initials: DM
authors.lastname: Glover
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D005656:Fungal Proteins; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D004718:Saccharomycetales / Q000201:enzymology*; D008941:Spindle Apparatus; D013379:Substrate Specificity; D000097869:Polo-Like Kinase 1
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D017346:Protein Serine-Threonine Kinases; D020543:Proteome; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1