Something from (almost) nothing: the impact of multiple displacement amplification on microbial ecology.
Microbial ecology is a field that applies molecular techniques to analyze genes and communities associated with a plethora of unique environments on this planet. In the past, low biomass and the predominance of a few abundant community members have impeded the application of techniques such as PCR, microarray analysis and metagenomics to complex microbial populations. In the absence of suitable cultivation methods, it was not possible to obtain DNA samples from individual microorganisms. Recently, a method called multiple displacement amplification (MDA) has been used to circumvent these limitations by amplifying DNA from microbial communities in low-biomass environments, individual cells from uncultivated microbial species and active organisms obtained through stable isotope probing incubations. This review describes the development and applications of MDA, discusses its strengths and limitations and highlights the impact of MDA on the field of microbial ecology. Whole genome amplification via MDA has increased access to the genomic DNA of uncultivated microorganisms and low-biomass environments and represents a 'power tool' in the molecular toolbox of microbial ecologists.
Binga
Erik K
EK
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
Lasken
Roger S
RS
Neufeld
Josh D
JD
D004247:DNA / Q000032:analysis; D004463:Ecology / Q000379:methods*; D017753:Ecosystem; D005827:Genetics, Microbial / Q000379:methods*; D023281:Genomics / Q000379:methods; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
D004247:DNA