Anticonvulsive effects of intracerebroventricular administration of rutin in rats.
Various synthetic derivatives of natural flavonoids are known to have neuroactive properties. The aim of the present study was to investigate the anticonvulsant effects of rutin (3, 3', 4', 5, 7-pentahydrohyflavone-3-rhamnoglucoside), a flavonoid which is an important dietary constituent of food and plant-based beverages. To this end, we assessed the anticonvulsant effects of rutin in rats treated with pentylenetetrazole (PTZ) (90 mg/kg, i.p.) and sought to clarify this mechanism. Intracerebroventricular (i.c.v.) injection of rutin dose-dependently affected minimal clonic seizures (MCS) and generalized tonic-clonic seizures (GTCS) induced by PTZ, through increments in seizure onset. Additionally, pretreatment with flumazenil (5 nM, i.c.v.) abolished the anticonvulsant effects of rutin during the onset of both seizures. These results indicate that rutin has anticonvulsant effects in the brain, possibly through positive allosteric modulation of the GABA(A) receptor complex via interaction at the benzodiazepine site.
Nassiri-Asl
Marjan
M
Department of Pharmacology, School of Medical Sciences, Qazvin University, Qazvin, Iran. marjannassiriaslm@gmail.com
Shariati-Rad
Schwann
S
Zamansoltani
Farzaneh
F
D000818:Animals; D000927:Anticonvulsants; D001921:Brain / Q000473:pathology; D003292:Convulsants; D004305:Dose-Response Relationship, Drug; D004830:Epilepsy, Tonic-Clonic / Q000139:chemically induced / Q000503:physiopathology; D005442:Flumazenil / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D018757:GABA Modulators / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D010433:Pentylenetetrazole; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000008:administration & dosage / Q000494:pharmacology*; D012640:Seizures / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D000927:Anticonvulsants; D003292:Convulsants; D018756:GABA Antagonists; D018757:GABA Modulators; D005442:Flumazenil; D005680:gamma-Aminobutyric Acid; D012431:Rutin; D010433:Pentylenetetrazole