Characterization of progressive periodontal lesions in chronic periodontitis patients: levels of chemokines, cytokines, matrix metalloproteinase-13, periodontal pathogens and inflammatory cells.
OBJECTIVE
Periodontitis is an infection with an episodic nature of tissue support destruction. The aim of this work was to determine the levels of chemokines, cytokines, matrix metalloproteinase-13, periodontal pathogens and inflammatory cells in periodontal sites characterized by active periodontal connective tissue destruction.


METHODS
Fifty-six patients with moderate or advanced severity of chronic periodontitis were selected. Periodontitis was characterized by at least six sites with probing depth > or =5 mm, clinical attachment level > or =3 mm and radiographic bone loss. Periodontitis progression was determined by the tolerance method. Receptor activator for nuclear factor kappa B-ligand (RANK-L), monocyte chemoattractant protein-1 (MCP-1), tumour necrosis factor-alpha (TNF-alpha), IL-1beta, MMP-13, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsithia and inflammatory cells levels were determined. Statistical analysis was performed using the Stata 7.0 software. Data were expressed as mean+/-SD and paired samples t-test and chi(2) tests were used.


RESULTS
Higher RANK-L, IL-1beta and MMP-13 activity levels were observed in active sites (p<0.05). The proportion of P. gingivalis, A. actinomycetemcomitans, T. forsythia and the number of CD4(+) T were higher in active than in inactive sites (p>0.05).


CONCLUSIONS
The detection of periodontopathic bacteria, host matrix metalloproteinases and cytokines in periodontitis patients with lesions undergoing episodic attachment loss could partially explain the mechanisms associated with the destruction of the supporting tissues of the tooth.
Silva
Nora
N
Area de Microbiologia, Dentistry School, University of Chile, Santiago, Chile.
Dutzan
Nicolas
N
Hernandez
Marcela
M
Dezerega
Andrea
A
Rivera
Oriana
O
Aguillon
Juan Carlos
JC
Aravena
Octavio
O
Lastres
Pedro
P
Pozo
Patricia
P
Vernal
Rolando
R
Gamonal
Jorge
J
D000328:Adult; D018932:Chemokine CCL2 / Q000032:analysis; D018925:Chemokines / Q000032:analysis*; D002908:Chronic Disease; D016207:Cytokines / Q000032:analysis*; D003773:Dental Plaque / Q000382:microbiology; D004812:Epidemiologic Methods; D005260:Female; D005881:Gingiva / Q000166:cytology / Q000601:surgery; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis*; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis; D010518:Periodontitis / Q000000981:diagnostic imaging / Q000201:enzymology / Q000382:microbiology; D053245:RANK Ligand / Q000032:analysis; D011859:Radiography; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D018932:Chemokine CCL2; D018925:Chemokines; D016207:Cytokines; D053583:Interleukin-1beta; D010446:Peptide Fragments; D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha; C508645:monocyte chemoattractant protein 1 (66-77); D053509:Matrix Metalloproteinase 13