title: Recombineering: genetic engineering in bacteria using homologous recombination.
abstract: The bacterial chromosome and plasmids can be engineered in vivo by homologous recombination using PCR products and synthetic oligonucleotides as substrates. This is possible because bacteriophage-encoded recombination functions efficiently to recombine sequences with homologies as short as 35 to 40 bases. This recombineering allows DNA sequences to be inserted or deleted without regard to location of restriction sites. This unit first describes preparation of electrocompetent cells expressing the recombineering functions and their transformation with dsDNA or ssDNA. Support protocols describe a two-step method of making genetic alterations without leaving any unwanted changes, and a method for retrieving a genetic marker (cloning) from the E. coli chromosome or a co-electroporated DNA fragment and moving it onto a plasmid. A method is also given to screen for unselected mutations. Additional protocols describe removal of defective prophage, methods for recombineering.
authors.affiliation: National Cancer Institute at Frederick, Frederick, Maryland, USA.
authors.forename: Lynn
authors.initials: L
authors.lastname: Thomason
authors.forename: Donald L
authors.initials: DL
authors.lastname: Court
authors.forename: Mikail
authors.initials: M
authors.lastname: Bubunenko
authors.forename: Nina
authors.initials: N
authors.lastname: Costantino
authors.forename: Helen
authors.initials: H
authors.lastname: Wilson
authors.forename: Simanti
authors.initials: S
authors.lastname: Datta
authors.forename: Amos
authors.initials: A
authors.lastname: Oppenheim
mesh_terms: D001419:Bacteria / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D018390:Gene Targeting / Q000379:methods; D005818:Genetic Engineering / Q000379:methods* / Q000639:trends*; D011995:Recombination, Genetic / Q000502:physiology*; D014169:Transformation, Bacterial / Q000235:genetics