title: Cis-9, trans-11-conjugated linoleic acid but not its precursor trans-vaccenic acid attenuate inflammatory markers in the human colonic epithelial cell line Caco-2.
abstract: Trans-vaccenic acid (TVA) is a natural trans fatty acid found in ruminant food produce. It is converted to the cis-9, trans-11 isomer of conjugated linoleic acid (c9, t11-CLA) by the action of stearoyl-CoA desaturase (SCD) in tissue. c9, t11-CLA has been associated with anti-inflammatory effects and also affects lipid metabolism. The aim of the present study was to determine if TVA is bioconverted to c9, t11-CLA in intestinal epithelial cells and to ascertain whether TVA has effects similar to c9, t11-CLA on markers of inflammation relevant to inflammatory bowel disease. The present study demonstrated that TVA treatment led to significant bioconversion into c9, t11-CLA in Caco-2 cells. Treatment with both TVA and c9, t11-CLA resulted in alteration of cellular fatty acid profile and SCD activity in the Caco-2 cell line. However, CLA, but not TVA, significantly modulated transcription of TNF-alpha, IL-12, IL-6 and production of IL-12 by these cells. Thus the present study established that TVA treatment can alter SCD desaturation indices and induce compositional changes in the fatty acid profile of the Caco-2 cell model of the human intestinal epithelium but this is not associated with functional effects on markers of the inflammatory response.
authors.affiliation: Nutrigenomics Research Group, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin, Republic of Ireland.
authors.forename: Clare M
authors.initials: CM
authors.lastname: Reynolds
authors.forename: Christine E
authors.initials: CE
authors.lastname: Loscher
authors.forename: Aidan P
authors.initials: AP
authors.lastname: Moloney
authors.forename: Helen M
authors.initials: HM
authors.lastname: Roche
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D018938:Caco-2 Cells; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D009829:Oleic Acids / Q000378:metabolism / Q000494:pharmacology*; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D005227:Fatty Acids; D018836:Inflammation Mediators; D044243:Linoleic Acids, Conjugated; D009829:Oleic Acids; C503589:cis-9, trans-11-conjugated linoleic acid; D013230:Stearoyl-CoA Desaturase; C050413:11-octadecenoic acid