[Prokaryotic expression and detective application of the main antigenic region of VP2 protein of Aleutian mink disease parvovirus].
To research safer diagnosis antigen for ADV, the main antigenic region VP2a and VP2b gene of ADV were obtained by restriction digestion of the recombinant plasmids pMD-VP2a and pMD-VP2b. Then the genes were respectively cloned into pMAL-c2 to get two prokaryotic recombinant plasmids pMAL-VPa and pMAL-VPb. The target genes were successfully expressed in the host cell TB1 when induced by IPTG. The Western blot analysis proved the recombinant proteins have good antigenic. The recombinant proteins were purified by KCL dyeing method, and were used as antigen to establish VP2-CIEP for AD diagnoses. The detection result shared 94.3% identity with that of CIEP. The results reported here show that VP2-CIEP is highly sensitive and specific and can benefit the research on the serodiagnosis to AD.
Zeng
Xiang-Wei
XW
Key Laboratory of Wildlife Conservation, Northeast Forestry University, Harbin 150040, China. xiangwei_zeng@163.com
Hua
Yu-Ping
YP
Liang
Dong-Ying
DY
D000453:Aleutian Mink Disease / Q000175:diagnosis; D000454:Aleutian Mink Disease Virus / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D003378:Counterimmunoelectrophoresis; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
D000956:Antigens, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C442555:VP2 protein, Aleutian mink disease virus