title: Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos.
abstract: The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopenta osylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.
authors.affiliation: Department of Developmental Biology, National Research Institute for Child Health and Development, 2-1 0-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
authors.forename: Ban
authors.initials: B
authors.lastname: Sato
authors.forename: Yohko U
authors.initials: YU
authors.lastname: Katagiri
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyado
authors.forename: Hidenori
authors.initials: H
authors.lastname: Akutsu
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Miyagawa
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakajima
authors.forename: Hajime
authors.initials: H
authors.lastname: Okita
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Hata
authors.forename: Junichiro
authors.initials: J
authors.lastname: Fujimoto
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Toshimori
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Kiyokawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism*; D001757:Blastomeres / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006028:Glycosphingolipids / Q000378:metabolism*; D000882:Haplorhini; D006801:Humans; D008297:Male; D051379:Mice; D055746:Stage-Specific Embryonic Antigens
chemical_list: D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4