title: COL1A2 gene polymorphisms (Pvu II and Rsa I), serum calciotropic hormone levels, and dental fluorosis.
abstract: OBJECTIVE
  To investigate the relationship between dental fluorosis, polymorphisms in the COL1A2 gene, and serum calciotropic hormone levels.
  
  
  METHODS
  We conducted a case-control study among children between 8 and 12 years of age with (n = 75) and without (n = 165) dental fluorosis in two counties in Henan Province, China. The PvuII and RsaI polymorphisms in the COL1A2 gene were genotyped using the PCR-RFLP procedure. Calcitonin and osteocalcin levels in the serum were measured using radioimmunassays.
  
  
  RESULTS
  Children carrying the homozygous genotype PP of COL1A2 PvuII had a significantly increased risk of dental fluorosis (OR =4.85, 95% CI: 1.22-19.32) compared to children carrying the homozygous genotype pp in an endemic fluorosis village (EFV). However, the risk (OR = 1.07, 95% CI: 0.45-2.52) was not elevated when the control population was recruited from a non-endemic fluorosis village. Additionally, fluoride levels in urine and osteocalcin levels in serum were found to be significantly lower in controls from non-endemic villages compared to cases. However, the differences in fluoride and osteocalcin levels were not observed when cases were compared to a control population from endemic fluorosis villages.
  
  
  CONCLUSIONS
  This study provides the first evidence of an association between polymorphisms in the COL1A2 gene with dental fluorosis in high fluoride exposed populations. Future studies are needed to confirm the association.
authors.affiliation: Department of Environmental Health, Zhengzhou University School of Public Health, Zhengzhou, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ba
authors.forename: Liuxin
authors.initials: L
authors.lastname: Cui
authors.forename: Xuemin
authors.initials: X
authors.lastname: Cheng
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Zhu
authors.forename: Yuling
authors.initials: Y
authors.lastname: Zhang
authors.forename: Pingping
authors.initials: P
authors.lastname: Yan
authors.forename: Cairong
authors.initials: C
authors.lastname: Zhu
authors.forename: Briseis
authors.initials: B
authors.lastname: Kilfoy
authors.forename: Yawei
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002116:Calcitonin / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002681:China / Q000453:epidemiology; D003094:Collagen / Q000235:genetics*; D024042:Collagen Type I; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D009050:Fluorosis, Dental / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D015675:Osteocalcin / Q000097:blood; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011347:Procollagen / Q000235:genetics
chemical_list: D024042:Collagen Type I; D005819:Genetic Markers; D011347:Procollagen; D015675:Osteocalcin; D002116:Calcitonin; D003094:Collagen; C047084:DNA modification methylase PvuII; D015257:DNA-Cytosine Methylases; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases