title: Nanocrystalline diamond--an excellent platform for life science applications.
abstract: Nanocrystalline diamond (NCD) has recently been successfully utilized in a variety of life science applications. NCD films are favorable and salubrious substrates for cells during cultivation. Therefore NCD has also been employed in tissue engineering strategies. NCD as reported in this contribution was grown by means of a modified hot-filament chemical vapor deposition technique, which results in less than 3% sp2-hybridization and yields grain sizes of 5-20 nm. After production the NCD surface was rather hydrophobic, however it could be efficiently refined to exhibit more hydrophilic properties. Changing of the surface structure was found to be an efficient means to influence growth and differentiation capacity of a variety of cells. The particular needs for any given cell type has to be proven empirically. Yet flexible features of NCD appear to be superior to plastic surfaces which can be hardly changed in quality. Besides its molecular properties, crystal structural peculiarities of NCD appear to influence cell growth as well. In our attempt to facilitate, highly specialized applications in biomedicine, we recently discovered that growth factors can be tightly bound to NCD by mere physisorption. Hence, combination of surface functionalization together with further options to coat NCD with any kind of three-dimensional structure opens up new avenues for many more applications. In fact, high through-put protein profiling of early disease stages may become possible from serum samples, because proteins bound to NCD can now be efficiently analyzed by MALDI/TOF-MS. Given these results, it is to be presumed that the physical properties and effective electrochemical characteristics of NCD will allow tailoring devices suitable for many more diagnostic as well as therapeutic applications.
authors.affiliation: Department for Cranio-Maxillofacial and Oral Surgery, Medical University of Innsbruck, Maximilianstr. 10, 6020 Innsbruck, Austria.
authors.forename: Frank R
authors.initials: FR
authors.lastname: Kloss
authors.forename: Muhammed
authors.initials: M
authors.lastname: Najam-Ul-Haq
authors.forename: Matthias
authors.initials: M
authors.lastname: Rainer
authors.forename: Robert
authors.initials: R
authors.lastname: Gassner
authors.forename: Günter
authors.initials: G
authors.lastname: Lepperdinger
authors.forename: Christian W
authors.initials: CW
authors.lastname: Huck
authors.forename: Günther
authors.initials: G
authors.lastname: Bonn
authors.forename: Frederik
authors.initials: F
authors.lastname: Klauser
authors.forename: Xianjie
authors.initials: X
authors.lastname: Liu
authors.forename: Norbert
authors.initials: N
authors.lastname: Memmel
authors.forename: Erminald
authors.initials: E
authors.lastname: Bertel
authors.forename: Jose A
authors.initials: JA
authors.lastname: Garrido
authors.forename: Doris
authors.initials: D
authors.lastname: Steinmüller-Nethl
mesh_terms: D002478:Cells, Cultured; D003460:Crystallization; D018360:Crystallography, X-Ray; D018130:Diamond / Q000737:chemistry*; D004563:Electrochemistry; D053758:Nanoparticles; D011506:Proteins / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D023822:Tissue Engineering
chemical_list: D011506:Proteins; D018130:Diamond