[To study the effect of oral mucosa cell transfected with IFN-gamma recover denuded hard palate to the growth of maxilla].
OBJECTIVE
To study the effect of oral mucosa cell transfected with IFN-gamma recover denuded hard palate to the growth of maxilla in rats.


METHODS
Divide 3-week-age 80 female rats with left denuded hard palate into 4 groups randomly, depends on different methods recovered the denuded hard palate, granulation tissue growing itselves (I), transplanting amnion (II), transplanting oral mucous cell loaded by amnion (III), or oral mucous cell transfected with IFN-gamma (IV), 20 rats in one group. After 9 weeks, measure the width of left hard palate and the right, and analyse the width of hard palate by different methods.


RESULTS
The antisymmetry rate of hard palate width of groups I, II, III and IV are (68.64 +/- 9.03)%, (58.53 +/- 7.40)%, (53.12 +/- 4.92)% and (52.25 +/- 4.61)%, statistical analysis through SNK of SPSS 11.0 show group I is different from groups II, III, IV (P < 0.05), group II is different from groups III, IV (P < 0.05), but group III is not different from group IV (P > 0.05).


CONCLUSIONS
It has energetic significance to prevent maxillary secondary deformity with amnion and tissue-engineered oral mucosa recover denuded hard palate, the tissue-engineered oral mucosa is better than amnion, the tissue-engineered oral mucosa transfected with IFN-gamma is better than not transfected.
Song
Qing-gao
QG
Department of Oral Maxillofacial Surgery of Zunyi Medical College, Zunyi 563003, China.
Shi
Bing
B
Huang
Lei
L
Zheng
Qian
Q
Wang
Yan
Y
D000818:Animals; D002972:Cleft Palate / Q000601:surgery*; D005260:Female; D007371:Interferon-gamma / Q000235:genetics*; D008437:Maxilla / Q000002:abnormalities / Q000601:surgery; D008445:Maxillofacial Development; D009061:Mouth Mucosa / Q000637:transplantation*; D019647:Oral Surgical Procedures; D021362:Palate, Hard / Q000254:growth & development; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology; D013524:Surgical Flaps; D023822:Tissue Engineering; D014162:Transfection
D007371:Interferon-gamma