Quantitative analysis of metabolic gases by multichannel Raman spectroscopy: use of a newly designed elliptic-spherical integration type of cell holder.
We describe the quantitative analysis of some metabolic gases bymultichannel Raman spectroscopy. Raman spectra were measured forair, acetone, ammonia, carbon dioxide, and mixed gas consisting ofacetone, ammonia, and air. We designed a new elliptic-sphericalintegration type of cell holder to obtain the Raman spectra of gaseswith a high signal-to-noise ratio. Concentrations of acetone, ammonia, and carbon dioxide were determined by the peak intensities ofRaman bands at 2940, 3228, and 1385 cm(-1), respectively. To compensate for the fluctuations of Ramanintensities caused by several factors, such as the fluctuations oflaser power, the peak intensity of a band at 2324 cm(-1) dueto nitrogen gas was used as an internal intensity standard. Thecorrelation coefficient between the corrected Raman intensity at 2940cm(-1) and the concentration of acetone was calculated to be0.984 for a concentration range of 2-12 ppm. The detection limitof acetone gas was found to be 2 ppm.
Yamamoto
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H
Uenoyama
H
H
Hirai
K
K
Dou
X
X
Ozaki
Y
Y