title: Importance of the high-molecular-mass isoform of adiponectin in improved insulin sensitivity with rosiglitazone treatment in HIV disease.
abstract: The present study was designed to investigate the relationship of isoforms of adiponectin to insulin sensitivity in subjects with HIV-associated insulin resistance in response to treatment with the thiazolidinedione, rosiglitazone. The two isoforms of adiponectin, HMW (high-molecular-mass) and LMW (low-molecular-mass), were separated by sucrose-gradient-density centrifugation. The amount of adiponectin in gradient fractions was determined by ELISA. Peripheral insulin sensitivity (Rd) was determined with hyperinsulinaemic-euglycaemic clamp, whereas hepatic sensitivity [HOMA (Homoeostasis Model Assessment) %S] was based on basal glucose and insulin values. Treatment with rosiglitazone for 3 months resulted in a significant improvement in the index of hepatic insulin sensitivity (86.4+/-15% compared with 139+/-23; P=0.007) as well as peripheral insulin sensitivity (4.04+/-0.23 compared with 6.17+/-0.66 mg of glucose/kg of lean body mass per min; P<0.001). Improvement in HOMA was associated with increased levels of HMW adiponectin (r=0.541, P=0.045), but not LMW adiponectin. The present study suggests that the HMW isoform of adiponectin is important in the regulation of rosiglitazone-mediated improvement in insulin sensitivity in individuals with HIV-associated insulin resistance, particularly in the liver.
authors.affiliation: Department of Medicine, Stony Brook University, Stony Brook, NY 11794, USA.
authors.forename: Saima
authors.initials: S
authors.lastname: Qurashi
authors.forename: Dennis C
authors.initials: DC
authors.lastname: Mynarcik
authors.forename: Margaret A
authors.initials: MA
authors.lastname: McNurlan
authors.forename: Hongshik
authors.initials: H
authors.lastname: Ahn
authors.forename: Robert
authors.initials: R
authors.lastname: Ferris
authors.forename: Marie C
authors.initials: MC
authors.lastname: Gelato
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000502:physiology; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D018791:CD4 Lymphocyte Count; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy* / Q000821:virology; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008099:Liver / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D020033:Protein Isoforms / Q000097:blood / Q000502:physiology; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000627:therapeutic use*; D019562:Viral Load
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D020033:Protein Isoforms; D045162:Thiazolidinediones; D000077154:Rosiglitazone