title: Canine distemper virus strains circulating among North American dogs.
abstract: Canine distemper virus (CDV) is a highly contagious virus that causes multisystemic disease in dogs. We received seven samples from dogs with CD from the United States during 2007. CDV isolates from these samples formed large, multinucleated syncytia in a Vero cell line expressing canine signaling lymphocyte activation molecule (SLAM). Based on the hemagglutinin gene sequences, the CDV isolates from three states (California, Missouri, and Oklahoma) formed two CDV genetic groups: group I (major; six of seven isolates) consisted of CDV isolates closely related to the European wildlife lineage of CDV, and group II (minor; one of seven isolates) was genetically related to the Arctic-like lineage of CDV. However, both CDV groups were genetically different from the current vaccine strains that belong to the American-1 lineage of the old (1930 to 1950) CDV isolates.
authors.affiliation: Oklahoma Animal Disease Diagnostic Laboratory, Center for Veterinary Health Sciences, Farm and Ridge Road, Stillwater, OK 74078, USA. sanjay.kapil@okstate.edu
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kapil
authors.forename: Robin W
authors.initials: RW
authors.lastname: Allison
authors.forename: Larry
authors.initials: L
authors.lastname: Johnston
authors.forename: Brandy L
authors.initials: BL
authors.lastname: Murray
authors.forename: Steven
authors.initials: S
authors.lastname: Holland
authors.forename: Jim
authors.initials: J
authors.lastname: Meinkoth
authors.forename: Bill
authors.initials: B
authors.lastname: Johnson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004216:Distemper / Q000453:epidemiology / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs; D005838:Genotype; D009656:North America / Q000453:epidemiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014709:Vero Cells