title: The toxicity of the quaternary ammonium compound benzalkonium chloride alone and in mixtures with other anionic compounds to bacteria in test systems with Vibrio fischeri and Pseudomonas putida.
abstract: Mixtures of chemicals are present in the aquatic environment but standard testing methods assess only single compounds. One aspect of this question is the importance of the formation of ionic pairs, for example from quaternary ammonium compounds with organic anions, and the significance of the ionic pairs for bacterial toxicity in the aquatic environment. The aim of the present study was to investigate the toxicity of the cationic quaternary ammonium compound benzalkonium chloride (BAC) against aquatic bacteria in the presence of substances commonly found in wastewater, such as the anionic surfactant linear alkylbenzene sulfonate (LAS), naphthalene sulfonic acid (NSA), sodium dodecylsulfonate (SDS), and benzene sulfonic acid (BSA). The growth inhibition test with Pseudomonas putida and the Vibrio fischeri luminescent inhibition test were used to determine the toxicity of single compounds and compound mixtures. The results found in this study indicate that ion pair formation is of minor significance under the test conditions applied here.
authors.affiliation: Institute of Environmental Medicine and Hospital Epidemiology, Freiburg University Hospital, Hugstetter Street 55, 79106 Freiburg, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Sütterlin
authors.forename: R
authors.initials: R
authors.lastname: Alexy
authors.forename: K
authors.initials: K
authors.lastname: Kümmerer
mesh_terms: D048908:Aliivibrio fischeri / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001548:Benzalkonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D016958:Pseudomonas putida / Q000187:drug effects*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D001548:Benzalkonium Compounds; D000644:Quaternary Ammonium Compounds