Isolation of epidermal cells and cDNA cloning of TNF decoy receptor 3 of conger eel, Conger myriaster.
By using EDTA and a trypsin solution, we established a method for isolating the epidermal cells of the conger eel, Conger myriaster. We then identified TNF decoy receptor (DcR) cDNA in the species from a suppression subtractive hybridization library prepared from the epidermal cells stimulated with LPS. The full-length cDNA of conger TNF DcR (conDcR) consisted of 1479 base pairs, and the protein comprised 286 amino acid residues. Phylogenetic analysis indicated that conDcR was clustered into a DcR3 branch. ConDcR is likely to act as an important immune-regulating factor in inhibiting the apoptosis-inducing effect of TNF in the skin of conger eel.
Tsutsui
Shigeyuki
S
School of Fisheries Science, Kitasato University, Okirai, Sanriku, Iwate 022-0101, Japan. tsutsui@kitasato-u.ac.jp <tsutsui@kitasato-u.ac.jp>
Yoshino
Yuko
Y
Matsui
Saho
S
Nakamura
Osamu
O
Muramoto
Koji
K
Watanabe
Tasuku
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004524:Eels / Q000235:genetics*; D000078404:Epidermal Cells; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D053319:Tumor Necrosis Factor Decoy Receptors / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D053319:Tumor Necrosis Factor Decoy Receptors