[Simultaneous identification of fifteen glucocorticoids in Chinese traditional medicine preparations by liquid chromatography-mass spectrometry].
To monitoring illegal abuse of these drugs, a liquid chromatography-mass spectrometry (LC-MS) method for identification of glucocorticoids in Chinese traditional medicine preparations was developed. Samples were extracted using different methods according to the nature of products. The analyses were performed on a Diamond C18 column (5 microm, 250 mm x 4.6 mm) with gradient elution using water-tetrahydrofuran (100 : 1, v/v) as mobile phase A and acetonitrile-water-tetrahydrofuran (80 : 20 : 1, v/v) as mobile phase B. Prednisone acetate in the preparation used to cure dermatitis was identified by comparing the retention time, full scan MS, and full scan MS2.
Xia
Rui
R
Beijing Municipal Institute of Drug Control, Beijing 100035, China. xia_rui@yahoo.com
Che
Baoquan
B
Zhang
Zhe
Z
D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005938:Glucocorticoids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal; D005938:Glucocorticoids