title: Glycosylation of S-RNases may influence pollen rejection thresholds in Solanum chacoense.
abstract: A survey of Solanum chacoense plants expressing an authentic S(11)-RNase transgene identified a line with partial compatibility to S(11) pollen. By comparing fruit set to the S-RNase levels determined immunologically in single styles, the minimum level of S(11)-RNase required for full rejection of S(11) pollen was estimated to be 18 ng per style. The S(11)-RNase threshold levels are thus considerably lower than those previously reported for the S(12)-RNase. Interestingly, these two allelic S-RNases differ dramatically in the extent of glycosylation, with the number of glycosylation sites varying from one (S(11)-RNase) to four (S(12)-RNase). It is suggested that reduced glycosylation of the S(11)-RNase may be related to the lower threshold for pollen rejection.
authors.affiliation: IRBV, Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke est, Montréal, Québec, Canada H1X 2B2.
authors.forename: Bolin
authors.initials: B
authors.lastname: Liu
authors.forename: David
authors.initials: D
authors.lastname: Morse
authors.forename: Mario
authors.initials: M
authors.lastname: Cappadocia
mesh_terms: D035264:Flowers / Q000201:enzymology*; D005838:Genotype; D006031:Glycosylation; D011058:Pollen / Q000502:physiology*; D054817:Pollination / Q000502:physiology*; D012260:Ribonucleases / Q000378:metabolism*; D032322:Solanum / Q000201:enzymology* / Q000235:genetics
chemical_list: D012260:Ribonucleases