title: Primary naive and interleukin-2-activated natural killer cells do not support efficient ectromelia virus replication.
abstract: Natural killer (NK) cells are known for their ability to lyse tumour cell targets. Studies of infections by a number of viruses, including poxviruses and herpesviruses, have demonstrated that NK cells are vital for recovery from these infections. Little is known of the ability of viruses to infect and complete a productive replication cycle within NK cells. Even less is known concerning the effect of infection on NK cell biology. This study investigated the ability of ectromelia virus (ECTV) to infect NK cells in vitro and in vivo. Following ECTV infection, NK cell gamma interferon (IFN-gamma) production was diminished and infected cells ceased proliferating and lost viability. ECTV infection of NK cells led to early and late virus gene expression and visualization of immature and mature virus particles, but no detectable increase in viable progeny virus. It was not unexpected that early gene expression occurred in infected NK cells, as the complete early transcription system is packaged within the virions. The detection of the secreted early virus-encoded immunomodulatory proteins IFN-gamma-binding protein and ectromelia inhibitor of complement enzymes (EMICE) in NK cell culture supernatants suggests that even semi-permissive infection may permit immunomodulation of the local environment.
authors.affiliation: Department of Molecular Microbiology and Immunology, Saint Louis University Health Sciences Center, 1402 South Grand Blvd, St Louis, MO 63104, USA.
authors.forename: April Keim
authors.initials: AK
authors.lastname: Parker
authors.affiliation: Howard Hughes Medical Institute, Rheumatology Division, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.
authors.forename: Wayne M
authors.initials: WM
authors.lastname: Yokoyama
authors.affiliation: Department of Biochemistry and Molecular Biology, Saint Louis University Health Sciences Center, 1402 South Grand Blvd, St Louis, MO 63104, USA.|Department of Molecular Microbiology and Immunology, Saint Louis University Health Sciences Center, 1402 South Grand Blvd, St Louis, MO 63104, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Corbett
authors.affiliation: Genelux Corporation, San Diego Science Center, 3030 Bunker Hill Street, Suite 310, San Diego, CA 92109, USA.
authors.forename: Nanhai
authors.initials: N
authors.lastname: Chen
authors.affiliation: Department of Molecular Microbiology and Immunology, Saint Louis University Health Sciences Center, 1402 South Grand Blvd, St Louis, MO 63104, USA.
authors.forename: R Mark L
authors.initials: RML
authors.lastname: Buller
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004481:Ectromelia virus / Q000472:pathogenicity* / Q000502:physiology; D004482:Ectromelia, Infectious / Q000821:virology; D005260:Female; D007376:Interleukin-2 / Q000276:immunology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014779:Virus Replication
chemical_list: D007376:Interleukin-2