title: Production of a complete cDNA library.
abstract: A complete cDNA library is one that contains at least one cDNA clone representing each mRNA in the cell. A method is provided in this unit for calculating the required base of a cDNA library such that the library should contain at least one copy of every mRNA. Either a phage or plasmid vector can be used in constructing a complete cDNA library, and protocols are described for both. Procedures for evaluating these cDNA libraries are also provided along with a procedure for evaluating the quality of the lambda arms used in constructing complete phage cDNA libraries.
authors.affiliation: Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors.forename: L B
authors.initials: LB
authors.lastname: Klickstein
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger