title: Chromosome instability in industrial strains of Saccharomyces cerevisiae batch cultivated under laboratory conditions.
abstract: Industrial ethanol fermentation is a complex microbiological process to which yeast cells must adapt for survival. One of the mechanisms for adaptation is thought to involve chromosome rearrangements. We found that changes in chromosome banding patterns measured by pulsed-field gel electrophoresis can also be produced in laboratory media under simulated industrial conditions. Based on analysis of their generational variation, we found that these chromosome changes were specific to the genetic backgrounds of the initial strains. We conclude that chromosome rearrangements could be one of the factors involved in yeast cell adaptation to the industrial environment.
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: B T L
authors.initials: BT
authors.lastname: Lucena
authors.forename: E A
authors.initials: EA
authors.lastname: Silva-Filho
authors.forename: M R M
authors.initials: MR
authors.lastname: Coimbra
authors.forename: J O F
authors.initials: JO
authors.lastname: Morais
authors.forename: D A
authors.initials: DA
authors.lastname: Simões
authors.forename: M A
authors.initials: MA
authors.lastname: Morais
mesh_terms: D000222:Adaptation, Physiological; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology; D043171:Chromosomal Instability; D015825:Chromosomes, Fungal / Q000235:genetics*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D007621:Karyotyping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
chemical_list: D004271:DNA, Fungal; D000431:Ethanol