title: Detection and analysis of proteins modified by O-linked N-acetylglucosamine.
abstract: The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.
authors.affiliation: The Johns Hopkins University Medical School, Baltimore, Maryland, USA.
authors.forename: Natasha E
authors.initials: NE
authors.lastname: Zachara
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Hart
authors.forename: Robert N
authors.initials: RN
authors.lastname: Cole
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000032:analysis*; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002236:Carbohydrate Conformation; D002478:Cells, Cultured / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D005700:Galactosyltransferases; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D006031:Glycosylation; D007202:Indicators and Reagents; D008322:Mammals / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D017351:N-Acetylglucosaminyltransferases / Q000032:analysis; D011499:Protein Processing, Post-Translational; D014909:Wheat Germ Agglutinins
chemical_list: D006023:Glycoproteins; D007202:Indicators and Reagents; C528213:N-acetylglucosaminylserine; C528214:N-acetylglucosaminylthreonine; D014909:Wheat Germ Agglutinins; C030717:succinylated wheat germ agglutinin; D005700:Galactosyltransferases; D017351:N-Acetylglucosaminyltransferases; C449880:O-GlcNAc transferase; D000117:Acetylglucosamine