title: Gene synthesis: assembly of target sequences using mutually priming long oligonucleotides.
abstract: This protocol uses pairs of oligonucleotides annealed at a short duplex segment at their 3' ends as both templates and primers (mutually primed synthesis) to generate desired sequences up to 400 bp in a single step. The procedure is divided into three sections: design of the oligonucleotides, extension by mutually primed synthesis, and cloning of the extension products. The strategy for design of oligonucleotides is discussed in the commentary.
authors.affiliation: Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Moore
mesh_terms: D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D005813:Genes, Synthetic; D005821:Genetic Techniques; D008967:Molecular Biology / Q000379:methods*
chemical_list: D004247:DNA