title: [15-deoxy-Delta(12,14)-prostaglandin J(2) induces anoikis of hepatocellular carcinoma cells: an in vitro experiment].
abstract: OBJECTIVE
  To investigate the effect of 15-deoxy-Delta(12,14)-prostaglandin J(2) (15-d-PGJ(2)) on the anoikis of hepatocellular carcinoma (HC) cells and mechanisms thereof.
  
  
  METHODS
  Fibronectin or polyhydroxyethyl methacrylate (poly-HEMA) were coated onto tissue culture plates, cell growth status and morphological changes were detected by optical microscope. DNA fragmentation analysis and Flow cytometry were used to measure cell apoptotic activity. Western blotting analysis was performed to detect the levels of focal adhesion kinase (FAK) and phosphorylated FAK(p-FAK). Furthermore, small interfering RNA (siRNA) was used to suppress FAK expression.
  
  
  RESULTS
  The adhesion rate of the BEL-7402 cells treated with 15-d-PGJ(2) began to decrease 12 h after the treatment, time- and dose-dependently compared with the HC cell control group (all P < 0.05); when the concentration of 15-d-PGJ2 was 20 micromol/L, the adherent cells ratio at 24 h and 48 h later were (66.0 +/- 3.6)% and (35.0 +/- 5.0)% respectively. Anoikis of BEL-7402 cells was observed by flow cytometry and DNA fragmentation analysis. Western blotting showed that the p-FAK level of the BEL-7402 cells treated with 15-d-PGJ2 for 24 h decreased dose-dependently, however, the total FAK protein did not change.
  
  
  CONCLUSIONS
  15-d-PGJ(2) induces anoikis and decreases the phosphorylated FAK expression of the hepatocellular carcinoma cells.
authors.affiliation: Department of Hepatobiliary Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Liang-Qi
authors.initials: LQ
authors.lastname: Cao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Chen
authors.forename: Mao-Chuan
authors.initials: MC
authors.lastname: Zhen
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Fu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Huang
mesh_terms: D023102:Anoikis / Q000187:drug effects*; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: C097240:15-deoxy-delta(12,14)-prostaglandin J2; D034741:RNA, Small Interfering; D051416:Focal Adhesion Protein-Tyrosine Kinases; D015230:Prostaglandin D2