[Expression of recombinant bovine beta-interferon by baculovirus system and evaluation of its bioactivity].
In this paper, a recombinant baculovirus containing the ORF of bovine interferon-beta (BoIFN-beta) gene, rBac-BoIFN-beta, was generated to express recombinant BoIFN-beta (rBoIFN-beta) in sf9 insect cells. The expression of rBoIFN-beta in rBac-BoIFN-beta infecting sf9 cells and its supernatants was confirmed by indirect immunofluorescence assay and Western blot. The antiviral activity of rBoIFN-beta in the supernatant can reach 10(6.0) AU/mL evaluated by the antiviral assay with VSV * GFP that expressed green fluorescence protein, and rBoIFN-beta could stimulate the expression of luciferase reporter gene controlled by chicken Mx promoter. All the results showed that rBac-BoIFN-beta constructed here could express high level recombinant BoIFN-beta in secreted form that had the bioactivity of natural type I IFN.
Chen
Wei-Ye
WY
College of Veterinary Medicine, Nanjing agricultural University, Nanjing 210095, China. chenweiye1980@yahoo.com.cn
Zhao
Hui-Jun
HJ
Wang
Xi-Jun
XJ
Huang
Ke-He
KH
Bu
Zhi-Gao
ZG
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D015153:Blotting, Western; D002417:Cattle; D019084:Fluorescent Antibody Technique, Indirect; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis*; D018411:Spodoptera
D011994:Recombinant Proteins; D016899:Interferon-beta