title: Whole-mount in situ hybridization and detection of RNAs in vertebrate embryos and isolated organs.
abstract: Nonisotopic in situ hybridization using intact embryos or organs is an important method for determining the spatial distribution of RNAs. Because it allows the analysis of large numbers of samples, it is amenable to temporal expression studies and comparison between different genotypes. It offers sensitivity and reproducibility. In addition, histological details are not lost during the staining process. The protocols in this unit can be used for whole-mount in situ hybridization in Xenopus, mouse, and chicken embryos, as well as dissected organs from mouse and chicken. Preparation of digoxigenin-labeled riboprobes is also described.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Pizard
authors.forename: Anna
authors.initials: A
authors.lastname: Haramis
authors.forename: Andrés E
authors.initials: AE
authors.lastname: Carrasco
authors.forename: Paula
authors.initials: P
authors.lastname: Franco
authors.forename: Silvia
authors.initials: S
authors.lastname: López
authors.forename: Alejandra
authors.initials: A
authors.lastname: Paganelli
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004076:Digoxigenin / Q000032:analysis / Q000276:immunology; D004622:Embryo, Mammalian / Q000737:chemistry* / Q000648:ultrastructure; D004625:Embryo, Nonmammalian / Q000737:chemistry* / Q000648:ultrastructure; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000276:immunology; D017403:In Situ Hybridization / Q000379:methods*; D007202:Indicators and Reagents; D051379:Mice; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D013045:Species Specificity; D013048:Specimen Handling / Q000379:methods; D014714:Vertebrates / Q000196:embryology; D014982:Xenopus laevis / Q000196:embryology
chemical_list: D007140:Immunoglobulin Fab Fragments; D007202:Indicators and Reagents; D015347:RNA Probes; D012333:RNA, Messenger; D004076:Digoxigenin