Liquid chromatography multi-stage mass spectrometry for the analysis of 5-hydroxymethylfurfural in foods.
A new, simple and selective method for the analysis of 5-hydroxymethylfurfural (HMF) in foods by liquid chromatography coupled to ion trap multi-stage mass spectrometry (LC-MS(n)) is proposed in the present study. Several chromatographic columns were tested and the best results were obtained using a phenyl fluorinated column. MS conditions were established using an atmospheric pressure chemical ionisation (APCI) source in the positive ionisation mode. MS/MS was used for quantitative analysis while MS(3) was required for confirmation purposes. Quality parameters such as day-to-day and run-to-run precision (RSD<10%) and detection limit (133 ng g(-1), 333 pg injected) were established. This method was successfully applied to the analysis of HMF content in several Spanish food samples from a local market.
Teixidó
Erika
E
Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
Moyano
Encarnación
E
Santos
F Javier
FJ
Galceran
M Teresa
MT
D002853:Chromatography, Liquid / Q000379:methods*; D005502:Food; D005504:Food Analysis; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry / Q000379:methods*
C008046:5-hydroxymethylfurfural; D005662:Furaldehyde