title: Complete genomic sequence and phylogenetic relatedness of hepatitis B virus isolates in Cambodia.
abstract: Although it is known that Cambodia is one of the high endemic area of hepatitis B virus (HBV) infection, molecular characterization of HBV circulating in this country has not been reported. In this study, pre-S gene of HBV from 12 Cambodian patients was sequenced. Phylogenetic analysis based on the pre-S gene sequence revealed that 8 out of 12 isolates (66.7%) belonged to HBV/C1 and remaining four (33.3%) were HBV/B4. Furthermore, complete genomic sequences were also determined for three Cambodian HBV isolates. They all comprised of 3,215 bp long and two of them belonged to subgenotype B4, which had recombination event with genotype C in the precore/core gene confirmed by SimPlot and BootScanning analyses. Our results showed that both HBV strains belonged to subgenotypes B4 and C1, which are circulating in this country. This is the first report on molecular characterization of the HBV prevalent in Cambodia.
authors.affiliation: Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
authors.forename: Tran Thien Tuan
authors.initials: TT
authors.lastname: Huy
authors.forename: Amadou Alpha
authors.initials: AA
authors.lastname: Sall
authors.forename: Jean Marc
authors.initials: JM
authors.lastname: Reynes
authors.forename: Kenji
authors.initials: K
authors.lastname: Abe
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002160:Cambodia / Q000453:epidemiology; D016679:Genome, Viral; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics*; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen