title: Preliminary crystallographic data of the three homologues of the thiol-disulfide oxidoreductase DsbA in Neisseria meningitidis.
abstract: Bacterial virulence depends on the correct folding of surface-exposed proteins, a process that is catalyzed by the thiol-disulfide oxidoreductase DsbA, which facilitates the synthesis of disulfide bonds in Gram-negative bacteria. Uniquely among bacteria, the Neisseria meningitidis genome possesses three genes encoding active DsbAs: DsbA1, DsbA2 and DsbA3. DsbA1 and DsbA2 have been characterized as lipoproteins involved in natural competence and in host-interactive biology, while the function of DsbA3 remains unknown. In an attempt to shed light on the reason for this multiplicity of dsbA genes, the three enzymes from N. meningitidis have been purified and crystallized in the presence of high concentrations of ammonium sulfate. The best crystals were obtained using DsbA1 and DsbA3; they belong to the orthorhombic and tetragonal systems and diffract to 1.5 and 2.7 A resolution, respectively.
authors.affiliation: Laboratoire des Protéines Membranaires, Institut de Biologie Structurale, CEA/CNRS/Université Joseph Fourier, 41 Rue Jules Horowitz, 38027 Grenoble CEDEX 01, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Lafaye
authors.forename: Thomas
authors.initials: T
authors.lastname: Iwema
authors.forename: Thomas
authors.initials: T
authors.lastname: Iwena
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Ferrer
authors.forename: J Simon
authors.initials: JS
authors.lastname: Kroll
authors.forename: Mickael
authors.initials: M
authors.lastname: Griat
authors.forename: Laurence
authors.initials: L
authors.lastname: Serre
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000201:enzymology*; D011487:Protein Conformation; D019704:Protein Disulfide-Isomerases / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D019704:Protein Disulfide-Isomerases