Production, characterization and (co-)immobilization of dextranase from Penicillium aculeatum.
Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stabilities. The larger enzyme was 3-fold more active (turnover number: 2,230 +/- 97 s(-1)). Pre-treatment of bentonite with H(2)O(2) did not affect adsorption characteristics of dextranase. Enzyme to bentonite ratios above 0.5:1 (w/w) resulted in a high conservation of activity upon adsorption. Furthermore, dextranase could be used in co-immobilizates for the direct conversion of sucrose into isomalto-oligosaccharides (e.g. isomaltose). Yields of co-immobilizates were 2-20 times that of basic immobilizates, which consist of dextransucrase without dextranase.
Erhardt
Frank A
FA
Department for Carbohydrate Technology, Institute for Technical Chemistry, Technical University Braunschweig, Hans-Sommer-Strasse 10, 38106, Braunschweig, Germany.
Stammen
Simon
S
Jördening
Hans-Joachim
HJ
D000327:Adsorption; D001546:Bentonite / Q000493:pharmacokinetics*; D002850:Chromatography, Gel; D003910:Dextranase / Q000096:biosynthesis* / Q000378:metabolism*; D003911:Dextrans / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005285:Fermentation; D005964:Glucosyltransferases / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000493:pharmacokinetics; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D007534:Isomaltose / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology*; D012965:Sodium Chloride / Q000493:pharmacokinetics; D013395:Sucrose / Q000378:metabolism
D003911:Dextrans; D004800:Enzymes, Immobilized; D007527:Isoenzymes; D001546:Bentonite; D012965:Sodium Chloride; D013395:Sucrose; D007534:Isomaltose; D006861:Hydrogen Peroxide; D005964:Glucosyltransferases; C020025:dextransucrase; D003910:Dextranase