Identification of phoslactomycin E as a metabolite inducing hyphal morphological abnormalities in Aspergillus fumigatus IFO 5840.
In our survey for antifungal compounds, a fermentation broth of Streptomyces sp. HA81-2 was found to inhibit the in vitro growth of Aspergillus fumigatus IFO 5840 accompanied by hyphal morphological abnormalities. One of the isolated antibiotics was identified as phoslactomycin E based on LC-MS and NMR spectral data. In a preliminary assay using the membrane fractions of A. fumigatus, phoslactomycin E was found to inhibit the activity of 1,3-beta glucan synthase.
Mizuhara
Naoko
N
Usuki
Yoshinosuke
Y
Ogita
Masaki
M
Fujita
Ken-Ichi
K
Kuroda
Manabu
M
Doe
Matsumi
M
Iio
Hideo
H
Tanaka
Toshio
T
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000166:cytology* / Q000187:drug effects* / Q000254:growth & development; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000037:antagonists & inhibitors; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors; D025301:Hyphae / Q000166:cytology* / Q000187:drug effects*; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013302:Streptomyces / Q000378:metabolism
D000935:Antifungal Agents; D003470:Culture Media; D005656:Fungal Proteins; D007783:Lactones; D009943:Organophosphorus Compounds; C060452:phoslactomycin E; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase