title: Impact of different bone marrow cell preparations on left ventricular remodelling after experimental myocardial infarction.
abstract: OBJECTIVE
  Bone marrow (BM)-derived haematopoietic stem cells have been proposed as a potential cell source to functionally engraft the myocardium and to improve cardiac function after myocardial infarction (MI). However, experimental and clinical data are inconsistent. Since the specific characteristics of different BM cell subsets could influence their therapeutic potential we determined the effect of different BM cell populations on left ventricular remodelling after MI.
  
  
  RESULTS
  MI was induced in female mice by coronary artery ligation. Surviving mice were randomised to receive either: total BM, mature Lin(+) or primitive Lin(-) cells from male mice, or saline, via intracardiac injection. Injected cells were detected in the infarct and border zone by PCR for Y-chromosomal sequences. Serial transthoracic echocardiography was performed 1, 21, and 42 days after MI. Over a period of 6 weeks, mortality was not different between the groups. After MI, animals exhibited left ventricular dilatation, as expected. Left ventricular remodelling was not influenced by Lin(+) or Lin(-) BM cells but was partially improved by unfractionated BM cell injection. Paracrine secretion of cytokines (e.g. IL-6, GM-CSF) was differentially regulated in supernatants of cultured BM cells.
  
  
  CONCLUSIONS
  Treatment with unfractionated BM cells, but not Lin(+), or Lin(-) cells partially improved cardiac remodelling and function after MI. This may be mediated by paracrine effects.
authors.affiliation: Medizinische Klinik und Poliklinik I, Universitätsklinikum, Julius-Maximilians-Universität Würzburg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Frantz
authors.forename: Duttu
authors.initials: D
authors.lastname: Vallabhapurapu
authors.forename: Jochen
authors.initials: J
authors.lastname: Tillmanns
authors.forename: Nikos
authors.initials: N
authors.lastname: Brousos
authors.forename: Helga
authors.initials: H
authors.lastname: Wagner
authors.forename: Kristina
authors.initials: K
authors.lastname: Henig
authors.forename: Georg
authors.initials: G
authors.lastname: Ertl
authors.forename: Albrecht M
authors.initials: AM
authors.lastname: Müller
authors.forename: Johann
authors.initials: J
authors.lastname: Bauersachs
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D005260:Female; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000401:mortality / Q000503:physiopathology / Q000628:therapy*; D019899:Paracrine Communication / Q000502:physiology; D016019:Survival Analysis; D020257:Ventricular Remodeling