title: Differential expression and dynamic changes of murine NEDD9 in progenitor cells of diverse tissues.
abstract: NEDD9 is a scaffolding protein in the integrin signaling pathway that is involved in cell adhesion dynamics. Little is known of the cellular localization of NEDD9 expression during embryonic development. In the present study, we have analyzed NEDD9 mRNA expression in the mouse and identified new relevant expression sites. In addition, we have characterized NEDD9 protein expression pattern for the first time in mammals. At E9.5-E10.5, high levels of Nedd9 and the neurogenic transcription factor neurogenin-2 (Ngn2) were found to largely overlap in two discrete domains of the trunk neural tube along its dorso-ventral axis, with Nedd9 extending to more ventral regions of the ventricular zone and Ngn2 differentially expressed in neuronally committed progenitors of the intermediate zone. At encephalic and trunk levels of the neural tube, NEDD9 was present in Sox2(+) progenitor cell populations mostly generating Ngn2(+) and/or Nurr1(+) cells. A sharp down-regulation of NEDD9 expression was found in cells upon lineage commitment, as observed in Nurr1(+) and Ngn2(+) mesencephalic dopaminergic and brainstem neuronal progenitors. In other tissues/organs, i.e. prospective heart, retina, olfactory epithelium, gonads, cartilage, gut and pituitary gland, NEDD9 was found to be co-expressed with Sox2, RXR alpha and/or Nurr1-like proteins, suggesting that NEDD9 expression is confined to early progenitors involved in diverse organogenesis and that it may depend on the repertoire and levels of retinoic acid co-receptors expressed by those cells.
authors.affiliation: Unit of Molecular Neurobiology, Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles vag 1 A1:2, 171 77 Stockholm, Sweden.
authors.forename: Jorge B
authors.initials: JB
authors.lastname: Aquino
authors.forename: Frédéric
authors.initials: F
authors.lastname: Marmigère
authors.forename: François
authors.initials: F
authors.lastname: Lallemend
authors.forename: T Kalle
authors.initials: TK
authors.lastname: Lundgren
authors.forename: Marcelo J
authors.initials: MJ
authors.lastname: Villar
authors.forename: Michael
authors.initials: M
authors.lastname: Wegner
authors.forename: Patrik
authors.initials: P
authors.lastname: Ernfors
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000378:metabolism; D015870:Gene Expression; D024002:HMGB Proteins / Q000032:analysis; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D054259:Neural Tube / Q000166:cytology / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D055748:SOXB1 Transcription Factors; D013234:Stem Cells / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; D024002:HMGB Proteins; C505592:NEDD9 protein, mouse; D011506:Proteins; D012333:RNA, Messenger; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors