Expression of endothelial cell-specific adhesion molecules in lungs after cardiac arrest.
OBJECTIVE
A method to compensate for donor shortages could be donation after cardiac death. In this study, we considered endothelial cell-specific molecules, claudin-5 and VE-cadherin, as possible biomarkers predicting lung injury against warm ischemia. We investigated how the expression of these molecules could change after cardiac arrest in a mouse lung, comparing other molecules presumably relating with ischemia.


METHODS
At given intervals after cardiac arrest, the lungs were harvested. Quantitative analysis of mRNA expression of claudin-5, VE-cadherin, IL-1beta, IL-10, HIF-alpha, Egr-1, VEGF, Ang-1 and Ang-2 genes in lung tissues with several periods of warm ischemia was performed.


RESULTS
Regarding endothelial cell-specific molecules, there were significant differences in both claudin-5 and VE-cadherin mRNA expression between 0 h and 4 h after cardiac arrest. IL-1beta mRNA expression 1 h, 2 h and 4 h after cardiac arrest increased significantly, compared with that at 0 h. There were no significant differences with the other genes.


CONCLUSIONS
We found that it took more time for claudin-5 and VE-cadherin mRNA expression to change significantly than IL-1beta mRNA expression; therefore, endothelial cell-specific molecules, claudin-5 and VE-cadherin, might be no better candidates for clinical use than IL-1beta.
Chen
Fengshi
F
Department of Organ Preservation Technology, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawahara-cho, Kyoto 606-8507, Japan.
Kondo
Nobuyuki
N
Sonobe
Makoto
M
Fujinaga
Takuji
T
Wada
Hiromi
H
Bando
Toru
T
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D062507:Claudin-5; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000378:metabolism*; D006323:Heart Arrest / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D008168:Lung / Q000098:blood supply / Q000601:surgery*; D008171:Lung Diseases / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D016040:Lung Transplantation; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D052096:Warm Ischemia / Q000009:adverse effects*; D000099152:Cadherin 5
D015703:Antigens, CD; D015415:Biomarkers; D015820:Cadherins; D015815:Cell Adhesion Molecules; D062507:Claudin-5; D053583:Interleukin-1beta; D008565:Membrane Proteins; D012333:RNA, Messenger; D000099152:Cadherin 5; C491511:Cldn5 protein, mouse