title: Metabolism-dependent taxis towards (methyl)phenols is coupled through the most abundant of three polar localized Aer-like proteins of Pseudomonas putida.
abstract: Comparatively little is known about directed motility of environmental bacteria to common aromatic pollutants. Here, by expressing different parts of a (methyl)phenol-degradative pathway and the use of specific mutants, we show that taxis of Pseudomonas putida towards (methyl)phenols is dictated by its ability to catabolize the aromatic compound. Thus, in contrast to previously described chemoreceptor-mediated chemotaxis mechanisms towards benzoate, naphthalene and toluene, taxis in response to (methyl)phenols is mediated by metabolism-dependent behaviour. Here we show that P. putida differentially expresses three Aer-like receptors that are all polar-localized through interactions with CheA, and that inactivation of the most abundant Aer2 protein significantly decreases taxis towards phenolics. In addition, the participation of a sensory signal transduction protein composed of a PAS, a GGDEF and an EAL domain in motility towards these compounds is demonstrated. The results are discussed in the context of the versatility of metabolism-dependent coupling and the necessity for P. putida to integrate diverse metabolic signals from its native heterogeneous soil and water environments.
authors.affiliation: Department of Molecular Biology, Umeå University, SE-901 87 Umeå, Sweden.
authors.forename: Inga
authors.initials: I
authors.lastname: Sarand
authors.forename: Sofia
authors.initials: S
authors.lastname: Osterberg
authors.forename: Sofie
authors.initials: S
authors.lastname: Holmqvist
authors.forename: Per
authors.initials: P
authors.lastname: Holmfeldt
authors.forename: Eleonore
authors.initials: E
authors.lastname: Skärfstad
authors.forename: Rebecca E
authors.initials: RE
authors.lastname: Parales
authors.forename: Victoria
authors.initials: V
authors.lastname: Shingler
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016764:Cell Polarity; D002633:Chemotaxis; D015964:Gene Expression Regulation, Bacterial; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D000072236:Methyl-Accepting Chemotaxis Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D010636:Phenols / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D010636:Phenols