title: Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis.
abstract: QueD (previously named ykvK) is one of several enzymes involved in the biosynthesis of the hypermodified nucleoside queuosine. Queuosine is incorporated into tRNA at position 34 of four tRNAs: tRNA(His), tRNA(Asp), tRNA(Asn) and tRNA(Tyr). The crystallization and preliminary X-ray crystallographic studies of queD are described here. The recombinant protein from Bacillus subtilis was overproduced in Escherichia coli and crystallized using the hanging-drop vapor-diffusion method from 25% PEG 600, 100 mM NaCl and sodium citrate buffer pH 5.5 at 291 K. The crystals diffract to 3.6 A resolution and belong to the cubic space group F4(1)32, with unit-cell parameter a = 240.88 A.
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. cicmil@uiuc.edu
authors.forename: Nenad
authors.initials: N
authors.lastname: Cicmil
authors.forename: Lu
authors.initials: L
authors.lastname: Shi
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D009704:Nucleoside Q / Q000096:biosynthesis*; D011487:Protein Conformation
chemical_list: D017931:DNA Primers; D009704:Nucleoside Q