Determination of abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine concentration in human plasma by MALDI-TOF/TOF.
The interest in therapeutic drug monitoring (TDM) of antiretroviral drugs has grown significantly since highly active antiretroviral therapy (HAART) became a standard of care in clinical practice. TDM is useful to determine the best dosage regimen adapted to each patient. Here, we apply MALDI-TOF/TOF technology to quantify abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine in the plasma of HIV-infected patients, by standard additions analysis. Regression of standard additions was linear over the whole anti-HIV concentration range explored (1.00 x 10(-2)-1.00 pmol/microL). The absolute recovery ranged between 80% and 110%. Values of the drug concentration determined by MALDI-TOF/TOF were in the range of 1.00 x 10(-2)-1.00 pmol/microL. The limit of quantification value was 1.00 x 10(-2)pmol/microL for abacavir, amprenavir, didanosine, efavirenz, nevirapine, and stavudine.
Notari
Stefania
S
Istituto Nazionale per le Malattie Infettive IRCCS Lazzaro Spallanzani, Via Portuense 292, I-00149 Roma, Italy. notari@inmi.it <notari@inmi.it>
Mancone
Carmine
C
Alonzi
Tonino
T
Tripodi
Marco
M
Narciso
Pasquale
P
Ascenzi
Paolo
P
D000480:Alkynes; D019380:Anti-HIV Agents / Q000097:blood* / Q000627:therapeutic use; D023241:Antiretroviral Therapy, Highly Active; D048588:Benzoxazines / Q000097:blood; D002219:Carbamates / Q000097:blood; D003521:Cyclopropanes; D016049:Didanosine / Q000097:blood; D015224:Dideoxynucleosides / Q000097:blood; D016903:Drug Monitoring / Q000379:methods*; D005240:Feasibility Studies; D005663:Furans; D006801:Humans; D015394:Molecular Structure; D019829:Nevirapine / Q000097:blood; D018894:Reverse Transcriptase Inhibitors / Q000097:blood; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D018119:Stavudine / Q000097:blood; D013449:Sulfonamides / Q000097:blood; D000099227:Abacavir
D000480:Alkynes; D019380:Anti-HIV Agents; D048588:Benzoxazines; D002219:Carbamates; D003521:Cyclopropanes; D016049:Didanosine; D015224:Dideoxynucleosides; D005663:Furans; D019829:Nevirapine; D018894:Reverse Transcriptase Inhibitors; D018119:Stavudine; D013449:Sulfonamides; D000099227:Abacavir; C095108:amprenavir; C098320:efavirenz