title: Extraction of essential oil from Pimpinella anisum using supercritical carbon dioxide and comparison with hydrodistillation.
abstract: Supercritical fluid extraction (SFE) of essential oil from Pimpinella anisum, using carbon dioxide as a solvent is presented in this work. An orthogonal array design OA9 (3(4)) was applied to select the optimum extraction condition. The effects of pressure, temperature, dynamic extraction time and methanol volume on the extraction efficiency were investigated by the three-level orthogonal array design. Results show that pressure has a significant effect on the extraction efficiency. The extract obtained from P. anisum by using supercritical fluid extraction was compared with the essential oil obtained by hydrodistillation, considering both quantity and quality of the product. SFE products were found to be of markedly different composition, compared with the corresponding hydrodistilated oil. The total amount of extractable substances obtained in SFE (7.5%) is higher than that obtained by hydrodistillation (3.1%) and SFE is faster than hydrodistillation method.
authors.affiliation: Department of Chemistry, Tarbiat Modares University, Tehran, Iran. yyamini@modares.ac.ir
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Yamini
authors.forename: Nader
authors.initials: N
authors.lastname: Bahramifar
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Sefidkon
authors.forename: Mohamad Jamal
authors.initials: MJ
authors.lastname: Saharkhiz
authors.forename: Ehsan
authors.initials: E
authors.lastname: Salamifar
mesh_terms: D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D028042:Pimpinella / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D002245:Carbon Dioxide