title: Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using naphthalene-2,3-dicarboxaldehyde as fluorogenic agent.
abstract: In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatization of AAs with NDA and a limit of detection (LOD) of 40nM was reached for glycine. Finally, we suggested the applicability of this microdevice for the analysis of real biological samples such as a rat hippocampus microdialysate.
authors.affiliation: Institut des Nanotechnologies de Lyon, INL, CNRS UMR5270, Université de Lyon, F-69003 Lyon, France.
authors.forename: O
authors.initials: O
authors.lastname: Yassine
authors.forename: P
authors.initials: P
authors.lastname: Morin
authors.forename: O
authors.initials: O
authors.lastname: Dispagne
authors.forename: L
authors.initials: L
authors.lastname: Renaud
authors.forename: L
authors.initials: L
authors.lastname: Denoroy
authors.forename: P
authors.initials: P
authors.lastname: Kleimann
authors.forename: K
authors.initials: K
authors.lastname: Faure
authors.forename: J-L
authors.initials: JL
authors.lastname: Rocca
authors.forename: N
authors.initials: N
authors.lastname: Ouaini
authors.forename: R
authors.initials: R
authors.lastname: Ferrigno
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D005898:Glass; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism; D009281:Naphthalenes / Q000032:analysis* / Q000737:chemistry; D051381:Rats
chemical_list: D000596:Amino Acids; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde