title: Assessment of how pregnancy modifies plasma lead and plasma/whole blood lead ratio in ALAD 1-1 genotype women.
abstract: Pregnant women are one of the most sensitive populations to the toxic effects associated with lead (Pb) exposure. These effects are primarily associated with plasma Pb (Pb-P), which reflects the most rapidly exchangeable fraction of Pb in the bloodstream, and elevated maternal Pb-P may be more relevant to foetal Pb exposure than whole blood Pb (Pb-B). We investigated how pregnancy affects Pb-B, Pb-P and %Pb-P/Pb-B ratios without the influence of the delta-aminolevulinic acid dehydratase (ALAD) G177C polymorphism, which is a major genetic factor influencing Pb-B, Pb-P and %Pb-P/Pb-B ratios. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion in nine pregnant and 20 non-pregnant women, aged 18-33, environmentally exposed to Pb. Here, we included only women with ALAD 1-1 genotype. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. We found no differences in Pb-B (P > 0.05). However, pregnant women had a 2-fold increase in Pb-P and a 3-fold increase in %Pb-P/Pb-B (both P < 0.01) compared to non-pregnant women. These alterations in Pb concentrations associated with pregnancy are similar to those associated with different ALAD gene variants. We can now better appreciate how pregnancy affects foetal exposure to Pb without the influence of this important genetic factor.
authors.affiliation: Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Marcelo Freitas
authors.initials: MF
authors.lastname: Montenegro
authors.forename: Fernando
authors.initials: F
authors.lastname: Barbosa
authors.forename: Jose E
authors.initials: JE
authors.lastname: Tanus-Santos
mesh_terms: D000328:Adult; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000097:blood* / Q000633:toxicity; D005260:Female; D005333:Fetus / Q000378:metabolism; D005838:Genotype; D006801:Humans; D007854:Lead / Q000097:blood* / Q000633:toxicity; D008431:Maternal-Fetal Exchange; D012150:Polymorphism, Restriction Fragment Length; D000623:Porphobilinogen Synthase / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy; D018570:Risk Assessment
chemical_list: D004785:Environmental Pollutants; D007854:Lead; D000623:Porphobilinogen Synthase