Adhesion of MRP8/14 to amastigotes in skin lesions of Leishmania major-infected mice.
In murine experimental cutaneous leishmaniasis, parasite infection induces an accumulation of macrophages expressing migration inhibitory factor-related protein 8 (MRP8) and MRP14, two members of the S100 calcium-binding protein family. Although MRP8 and MRP14 are cytoplasmic proteins expressed by myeloid cells, recent studies have demonstrated that MRP8 and MRP14 have extracellular functions such as chemotactic activities. In this study, we examined whether extracellular MRP8 and MRP14 interact with Leishmania parasites during infection. By immunohistochemistry, positive staining by MRP8 and MRP14 was detected on amastigotes in skin lesions of Leishmania major-infected mice. Western blot analysis with amastigotes purified from the skin lesions demonstrated that both of these proteins adhered to amastigotes. The adhesion of MRP14 to amastigotes was reproduced in vitro and enhanced in the presence of Ca2+ and Zn2+. MRP14 adhered to not only amastigotes, but also promastigotes, suggesting receptor molecules for MRP14 are expressed commonly in both developmental stages.
Goto
Yasuyuki
Y
Department of Molecular Immunology, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113 8657, Japan.
Sanjoba
Chizu
C
Asada
Masahito
M
Saeki
Keiichi
K
Onodera
Takashi
T
Matsumoto
Yoshitsugu
Y
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D040501:Calgranulin A / Q000378:metabolism*; D040502:Calgranulin B / Q000378:metabolism*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D007150:Immunohistochemistry; D018320:Leishmania major / Q000378:metabolism*; D016773:Leishmaniasis, Cutaneous / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012867:Skin / Q000469:parasitology* / Q000473:pathology; D015032:Zinc / Q000378:metabolism
D040501:Calgranulin A; D040502:Calgranulin B; D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse; D015032:Zinc; D002118:Calcium