[Effect of TLR4 on expressions of p-IRAK1 and p-IkappaB-alpha in HPDLCs stimulated by lipopolysaccharide].
OBJECTIVE
To evaluate the effect of TLR4 on expressions of p-IRAK1 and p-IkappaB-alpha in HPDLCs stimulated by lipopolysaccharide.


METHODS
Western blot and image analysis were used to detect the levels of p-IRAK1 and p-IkappaB-alpha in HPDLCs stimulated by 1 microg/ml LPS after 2.5,5,10 and 15 minutes, and to detect the levels of these proteins in HPDLCs stimulated by 1 microg/ml LPS after pretreatment with monoclonal antibody of TLR4. Statistical analysis was performed with SPSS10.0 software package for one-way ANOVA.


RESULTS
The expressions of p-IRAK1 and p-IkappaB-alpha in HPDLCs were enhanced after stimulation of LPS. The gray scale values of protein band at 0 minute were 0.054, 0.19 respectively, significantly increased to 0.785, 0.809 after 5 minutes (P<0.05). After pretreatment with monoclonal antibody of TLR4, the expressions of these proteins weakened compared with those without pretreatment. The gray scale values of protein band significantly decreased from 0.82,0.874 to 0.099,0.201, respectively (P<0.05).


CONCLUSIONS
TLR4 is involved in the signaling transduction in HPDLCs stimulated with LPS, and might be involved in the progression of periodontis.
Sun
Ying
Y
Department of Periodontology, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, College of Stomatology, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Shu
Rong
R
Zhang
Ming-zhu
MZ
Li
Chao-lun
CL
D000911:Antibodies, Monoclonal; D006801:Humans; D020672:I-kappa B Proteins / Q000096:biosynthesis*; D053592:Interleukin-1 Receptor-Associated Kinases / Q000096:biosynthesis; D008070:Lipopolysaccharides; D000072000:NF-KappaB Inhibitor alpha; D010518:Periodontitis / Q000378:metabolism*; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D020672:I-kappa B Proteins; D008070:Lipopolysaccharides; C000606204:NFKBIA protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; C507768:IRAK1 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases