title: Overview of the baculovirus expression system.
abstract: Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.
authors.affiliation: Aquila Biopharmaceuticals, Worcester, Massachusetts, USA.
authors.forename: Cheryl Isaac
authors.initials: CI
authors.lastname: Murphy
authors.forename: Helen
authors.initials: H
authors.lastname: Piwnica-Worms
authors.forename: Stefan
authors.initials: S
authors.lastname: Grünwald
authors.forename: William G
authors.initials: WG
authors.lastname: Romanow
authors.forename: Nicole
authors.initials: N
authors.lastname: Francis
authors.forename: Hua-Ying
authors.initials: HY
authors.lastname: Fan
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D002460:Cell Line / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D007202:Indicators and Reagents; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology; D000077183:Occlusion Body Matrix Proteins; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D018411:Spodoptera / Q000166:cytology / Q000821:virology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral; D007202:Indicators and Reagents; D000077183:Occlusion Body Matrix Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus