Crystallization and preliminary crystallographic analysis of human Ca 2+-loaded calbindin-D28k.
Calbindin-D28k is a calcium-binding protein that belongs to the troponin C superfamily. It is expressed in many tissues, including brain, intestine, kidney and pancreas, and performs roles as both a calcium buffer and a calcium sensor and carries out diverse physiological functions of importance. In order to resolve the crystal structure of human calbindin-D28k and to gain a better understanding of its biological functions, recombinant human calbindin-D28k was crystallized at 291 K using PEG 3350 as precipitant and a 2.4 A resolution X-ray data set was collected from a single flash-cooled crystal (100 K). The crystal belonged to space group C2, with unit-cell parameters a = 108.1, b = 28.2, c = 70.6 A, beta = 107.8 degrees . The presence of one molecule per asymmetric unit is presumed, corresponding to a Matthews coefficient of 1.75 A(3) Da(-1).
Zhang
Chang
C
Tsinghua-Nankai-IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.
Sun
Yuna
Y
Wang
Wei
W
Zhang
Yan
Y
Ma
Ming
M
Lou
Zhiyong
Z
D001483:Base Sequence; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000737:chemistry*; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D064030:S100 Calcium Binding Protein G / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
C576498:CALB1 protein, human; D064092:Calbindin 1; D064026:Calbindins; D017931:DNA Primers; D011994:Recombinant Proteins; D064030:S100 Calcium Binding Protein G; D002118:Calcium