title: The role of electrosprayed apatite nanocrystals in guiding osteoblast behaviour.
abstract: Apatite nanocrystals, which mimic the dimensions of natural bone mineral, were electrosprayed on glass substrates, as a suitable synthetic biomedical material for osteoblast outgrowth was explored. A variety of topographic patterns were deposited and the influence of these designs on osteoblast alignment and cell differentiation was investigated. Patterned cell growth and enhanced cell differentiation were seen. Osteoblasts were also cultured on apatite nanocrystals chemically modified with either carbonate or silicon ions. Enhanced cell proliferation and early formation of mineral nodules were observed on apatite nanocrystals with silicon addition. This work highlights the importance of the combined effects of surface topography and surface chemistry in the guidance of cell behaviour.
authors.affiliation: Department of Materials Science and Metallurgy, University of Cambridge, Cambridge, UK. est23@cam.ac.uk
authors.forename: Eng
authors.initials: E
authors.lastname: San Thian
authors.forename: Zeeshan
authors.initials: Z
authors.lastname: Ahmad
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.forename: Mohan J
authors.initials: MJ
authors.lastname: Edirisinghe
authors.forename: Suwan N
authors.initials: SN
authors.lastname: Jayasinghe
authors.forename: Deborah C
authors.initials: DC
authors.lastname: Ireland
authors.forename: Roger A
authors.initials: RA
authors.lastname: Brooks
authors.forename: Neil
authors.initials: N
authors.lastname: Rushton
authors.forename: William
authors.initials: W
authors.lastname: Bonfield
authors.forename: Serena M
authors.initials: SM
authors.lastname: Best
mesh_terms: D000336:Aerosols / Q000737:chemistry; D001031:Apatites / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D010316:Particle Size; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000336:Aerosols; D001031:Apatites