title: 16S rRNA gene sequences from bacteria associated with adult Anopheles darlingi (Diptera: Culicidae) mosquitoes.
abstract: The microbial flora associated with Anopheles darlingi Root (Diptera: Culicidae), a major Neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in Brazil. Female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. The controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial DNA from all blood-fed mosquitoes. The 16S sequences of bacteria recovered, include some closely related to those found in other vector mosquitoes, including Aeromonas, Pantoea and Pseudomonas species.
authors.affiliation: Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, CA 92697, USA.
authors.forename: Olle
authors.initials: O
authors.lastname: Terenius
authors.forename: Caroline Dantas
authors.initials: CD
authors.lastname: de Oliveira
authors.forename: Waleria Dasso
authors.initials: WD
authors.lastname: Pinheiro
authors.forename: Wanderli Pedro
authors.initials: WP
authors.lastname: Tadei
authors.forename: Anthony Amade
authors.initials: AA
authors.lastname: James
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Marinotti
mesh_terms: D000818:Animals; D000852:Anopheles / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D008297:Male; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S