Mapping by partial endonuclease digestions.
In the procedure described in this unit, a DNA fragment radiolabeled at one of its two ends is purified by gel electrophoresis and subjected to partial cleavage by a restriction endonuclease. Analysis of the resulting products by polyacrylamide (or agarose) gel electrophoresis enables one to define the distance of restriction sites from the labeled end.
Bloch
K D
KD
Harvard Medical School, Boston, Massachusetts, USA.
D004247:DNA / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000378:metabolism*
D004247:DNA; D004720:Endonucleases