Acid hydrolysis for release of monosaccharides.
The first step in obtaining a compositional analysis of a glycoconjugate is the release of the individual monosaccharide constituents. Mild acid hydrolysis to release fucosyl residues from glycoconjugates is described in this unit, in addition to mild acid hydrolysis to release sialic acids from glycoconjugates, along with dialysis and column chromatography procedures to purify the sialic acids. Strong acid hydrolysis to release all monosaccharides from glycoconjugates is also detailed.
Manzi
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University of California San Diego, La Jolla, California, USA.
D000107:Acetylation; D000143:Acids / Q000737:chemistry*; D000818:Animals; D006001:Glycoconjugates / Q000737:chemistry; D006868:Hydrolysis; D009005:Monosaccharides / Q000737:chemistry*; D012794:Sialic Acids / Q000737:chemistry
D000143:Acids; D006001:Glycoconjugates; D009005:Monosaccharides; D012794:Sialic Acids