Combined use of sodium borocaptate and buthionine sulfoximine in boron neutron capture therapy enhanced tissue boron uptake and delayed tumor growth in a rat subcutaneous tumor model.
We have previously reported that buthionine sulfoximine (BSO) enhances sodium borocaptate (BSH) uptake by down regulating glutathione (GSH) synthesis in cultured cells. This study investigated the influence of BSO on tissue BSH uptake in vivo and the efficacy of BSH-BSO-mediated boron neutron capture therapy (BNCT) on tumor growth using a Fisher-344 rat subcutaneous tumor model. With BSO supplementation, boron uptake in subcutaneous tumor, blood, skin, muscle, liver, and kidney was significantly enhanced and maintained for 12h. Tumor growth was significantly delayed by using BSO. With further improvement in experimental conditions, radiation exposure time, together with radiation damage to normal tissues, could be reduced.
Yoshida
Fumiyo
F
University of Tsukuba, Graduate School of Comprehensive Human Sciences, Functional and Regulatory Medical Sciences, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan. yoshi234@sakura.cc.tsukuba.ac.jp
Yamamoto
Tetsuya
T
Nakai
Kei
K
Kumada
Hiroaki
H
Shibata
Yasushi
Y
Tsuruta
Wataro
W
Endo
Kiyoshi
K
Tsurubuchi
Takao
T
Matsumura
Akira
A
D000818:Animals; D001894:Borohydrides / Q000008:administration & dosage / Q000494:pharmacology*; D001895:Boron / Q000378:metabolism*; D016754:Boron Neutron Capture Therapy / Q000379:methods*; D019328:Buthionine Sulfoximine / Q000008:administration & dosage / Q000494:pharmacology*; D007554:Isotopes / Q000378:metabolism; D008297:Male; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000532:radiotherapy*; D051381:Rats; D011916:Rats, Inbred F344; D013438:Sulfhydryl Compounds / Q000008:administration & dosage / Q000494:pharmacology*
D001894:Borohydrides; D007554:Isotopes; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate; D019328:Buthionine Sulfoximine; D001895:Boron