title: Using haplotype probabilities in categorical survival analysis: a case study with three candidate genes in an Iberian x Meishan F2 population of newborn piglets.
abstract: Haplotype probabilities have been implemented in association analyses performed between three candidate genes and the preweaning survival of F2 Iberian x Meishan piglets, under a categorical proportional hazard model. The additive effect of substitution of each haplotype was estimated using information of parental genotypes for non-genotyped piglets. Analysed loci were the bone morphogenetic protein type 1B receptor (BMPR1B) gene at 8q25 (three SNPs; haplotypes BMPR1B A, BMPR1B B, BMPR1B C and BMPR1B D), the dopamine beta-hydroxylase (DBH) gene at 1q2.13 (two SNPs; haplotypes DBH X, DBH Y and DBH Z), and the prolactin receptor (PRLR) gene at 16q1.4-q2.3 (six SNPs; haplotypes PRLR A, PRLR B, PRLR C, PRLR D and PRLR E). The number of analysed records were 899 (BMPR1B), 850 (DBH) and 822 (PRLR) with a censoring percentage ranged between 87 and 89%. Associations with preweaning survival were suggested for BMPR1B (p < 0.10) and associations were clearly significant for DBH haplotypes (p < 0.05), whereas the PRLR locus did not reach statistical significance (p > 0.10). The BMPR1B A haplotype could increase piglet survivability in relation to the BMPR1B B haplotype with a hazard ratio of 0.751 and a confidence interval (CI) between 0.550 and 1.025 (p < 0.10). The two remaining haplotypes did not differ significantly from the BMPR1B B haplotype, with a hazard ratio higher than 0.90 (p > 0.10). For the DBH locus, the DBH X and the DBH Z haplotypes were associated with an improved preweaning survival in relation to the DBH Y haplotype. The advantage for DBH X in terms of hazard ratio was 0.789 (CI = 0.625 to 0.994; p < 0.05) and differences between DBH Y and DBH Z reached a hazard ratio of 0.770 (CI = 0.606 to 0.977; p < 0.05). Although further researches are required, BMPR1B and DBH genes could be a useful tool to improve piglet survival under a marker assisted selection strategy.
authors.affiliation: Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, Barcelona, Spain. joaquim.casellas@irta.es
authors.forename: J
authors.initials: J
authors.lastname: Casellas
authors.forename: A
authors.initials: A
authors.lastname: Tomás
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: E
authors.initials: E
authors.lastname: Alves
authors.forename: J L
authors.initials: JL
authors.lastname: Noguera
authors.forename: J
authors.initials: J
authors.lastname: Piedrafita
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics; D001947:Breeding; D003433:Crosses, Genetic; D004299:Dopamine beta-Hydroxylase / Q000235:genetics; D005260:Female; D006239:Haplotypes; D008297:Male; D020641:Polymorphism, Single Nucleotide; D016016:Proportional Hazards Models; D011981:Receptors, Prolactin / Q000235:genetics; D016019:Survival Analysis; D034421:Sus scrofa / Q000235:genetics*
chemical_list: D011981:Receptors, Prolactin; D004299:Dopamine beta-Hydroxylase; D052005:Bone Morphogenetic Protein Receptors, Type I