Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.
Hamady
Micah
M
Department of Computer Science, UCB 430, University of Colorado, Boulder, Colorado 80309, USA.
Walker
Jeffrey J
JJ
Harris
J Kirk
JK
Gold
Nicholas J
NJ
Knight
Rob
R
D017931:DNA Primers / Q000737:chemistry; D005815:Genetic Code; D012329:RNA, Bacterial / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000737:chemistry*; D017422:Sequence Analysis, DNA / Q000379:methods*
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S