Triterpenoid saponins with neuroprotective effects from the roots of Polygala tenuifolia.
The methanol fraction of an ethanolic extract from the roots of Polygala tenuifolia Willd. showed antagonistic action on neurotoxicity induced by glutamate and serum deficiency in PC12 cells. Bioassay-guided fractionation led to the isolation of six new triterpenoid saponins, onjisaponins V - Z, and Vg ( 1 - 6), together with ten known saponins ( 7 - 16). The structures of 1 - 6 were elucidated by spectroscopic and chemical methods. Screening results indicated that compounds 1 - 16 showed neuroprotective effects against serum deficiency and glutamate at the concentration of 10 (-5) mol/L.
Li
Chuangjun
C
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College (Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education), Beijing, China.
Yang
Jingzhi
J
Yu
Shishan
S
Chen
Naihong
N
Xue
Wei
W
Hu
Jingfeng
J
Zhang
Dongming
D
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D006868:Hydrolysis; D008516:Medicine, Chinese Traditional; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D018517:Plant Roots / Q000737:chemistry*; D031801:Polygala / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D003470:Culture Media; D018696:Neuroprotective Agents; D012503:Saponins; D014315:Triterpenes