Designing a whole-cell biotransformation system in Escherichia coli using cytochrome P450 from Streptomyces peucetius.
A biotransformation system was designed to co-express CYP107P3 (CSP4), cytochrome P450, from Streptomyces peuceticus, along with CamA (putidaredoxin reductase) and CamB (putidaredoxin) from Pseudomonas putida, the necessary reducing equivalents, in a class I type electron-transfer system in E. coli BL21 (DE3). This was carried out using two plasmids with different selection markers and compatible origins of replication. The study results showed that this biotransformation system was able to mediate the O-dealkylation of 7-ethoxycumarin.
Shrestha
Pramod
P
Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction (iBR), SunMoon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam, 336-708, Republic of Korea.
Oh
Tae-Jin
TJ
Sohng
Jae Kyung
JK
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism; D002384:Catalysis; D003374:Coumarins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003640:Dealkylation; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D005288:Ferredoxins / Q000378:metabolism; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010957:Plasmids; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D003374:Coumarins; D005288:Ferredoxins; C017299:7-ethoxycoumarin; C100137:putidaredoxin; D003577:Cytochrome P-450 Enzyme System; D009247:NADH, NADPH Oxidoreductases; C022486:putidaredoxin reductase