title: A genetic screen for Saccharomyces cerevisiae mutants affecting proteasome function, using a ubiquitin-independent substrate.
abstract: The great majority of proteasome substrates are marked for degradation by the attachment of polyubiquitin chains. Ornithine decarboxylase is degraded by the proteasome in the absence of this modification. We previously showed that this mechanism of degradation was conserved in eukaryotic cells. Here we use a reporter destabilized by mouse ornithine decarboxylase to screen non-essential Saccharomyces cerevisiae deletion mutants. We identified novel mutants that affect both ubiquitin-dependent and -independent proteasome degradation pathways. YLR021W (IRC25/POC3) and YPL144W (POC4) encode interacting proteins that function in proteasome assembly, with putative homologues widespread among eukaryotes. Several additional mutants suffered from defects in proteasome-mediated proteolysis. These included mutants in the urmylation pathway of protein modification (but not the Urm1 modifier itself) and the Reg1 regulatory subunit of protein phosphatase 1. Finally, we noted increased rates of ornithine decarboxylase turnover in an rpn10Delta mutant in which the degradation of certain ubiquitinated substrates is impaired. Together, these results highlight the utility of a ubiquitin-independent degron in uncovering novel factors affecting general and substrate-specific proteasome function.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, CA, 94143, USA.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Hoyt
authors.forename: Stephen
authors.initials: S
authors.lastname: McDonough
authors.forename: Stephan A
authors.initials: SA
authors.lastname: Pimpl
authors.forename: Hartmut
authors.initials: H
authors.lastname: Scheel
authors.forename: Kay
authors.initials: K
authors.lastname: Hofmann
authors.forename: Philip
authors.initials: P
authors.lastname: Coffino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051379:Mice; D018832:Molecular Chaperones / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion; D013379:Substrate Specificity; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex; D009955:Ornithine Decarboxylase