URP2SF, a FERM and PH domain containing protein, regulates NF-kappaB and apoptosis.
The transcription factor NF-kappaB plays an important role in inflammation and cell survival. Here, we report the functional characterization of URP2SF (UNC-112 related protein 2 short form), which is a novel protein containing FERM and PH domains. The protein is conserved in evolution across different species. Northern blot and RT-PCR analysis revealed that the expression of URP2SF appeared to be primarily confined to immune system. URP2SF protein localized to the whole cell. Over-expression of URP2SF strongly repressed NF-kappaB activity in the absence or presence of NF-kappaB stimulus. Importantly, URP2SF over-expression also induced cell apoptosis. Furthermore, suppression of URP2SF expression by small interference RNA significantly activated the NF-kappaB reporter gene activities, as well as attenuated TNF-alpha induced apoptosis. Therefore, our data show for the first time that URP2SF may act as a transcriptional repressor in NF-kappaB signaling pathway and regulate cell apoptotic pathway.
Wang
Lan
L
Chinese National Human Genome Center, No. 3-707 North YongChang Road BDA, Beijing 100176, PR China.
Deng
Weiwei
W
Shi
Taiping
T
Ma
Dalong
D
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D008565:Membrane Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000096:biosynthesis*; D009363:Neoplasm Proteins / Q000502:physiology*; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D020298:U937 Cells
C477322:FERMT3 protein, human; D008565:Membrane Proteins; D016328:NF-kappa B; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D014409:Tumor Necrosis Factor-alpha