title: Direct multiplexed measurement of gene expression with color-coded probe pairs.
abstract: We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels.
authors.affiliation: NanoString Technologies Inc., 201 Elliott Ave West, Suite 300, Seattle, Washington 98119, USA. ggeiss@nanostring.com
authors.forename: Gary K
authors.initials: GK
authors.lastname: Geiss
authors.forename: Roger E
authors.initials: RE
authors.lastname: Bumgarner
authors.forename: Brian
authors.initials: B
authors.lastname: Birditt
authors.forename: Timothy
authors.initials: T
authors.lastname: Dahl
authors.forename: Naeem
authors.initials: N
authors.lastname: Dowidar
authors.forename: Dwayne L
authors.initials: DL
authors.lastname: Dunaway
authors.forename: H Perry
authors.initials: HP
authors.lastname: Fell
authors.forename: Sean
authors.initials: S
authors.lastname: Ferree
authors.forename: Renee D
authors.initials: RD
authors.lastname: George
authors.forename: Tammy
authors.initials: T
authors.lastname: Grogan
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: James
authors.forename: Malini
authors.initials: M
authors.lastname: Maysuria
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Mitton
authors.forename: Paola
authors.initials: P
authors.lastname: Oliveri
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Osborn
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Amber L
authors.initials: AL
authors.lastname: Ratcliffe
authors.forename: Philippa J
authors.initials: PJ
authors.lastname: Webster
authors.forename: Eric H
authors.initials: EH
authors.lastname: Davidson
authors.forename: Leroy
authors.initials: L
authors.lastname: Hood
authors.forename: Krassen
authors.initials: K
authors.lastname: Dimitrov
mesh_terms: D002460:Cell Line; D003116:Color; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D015723:Gene Library; D017930:Genes, Reporter; D006801:Humans; D007091:Image Processing, Computer-Assisted; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D017955:Poliovirus; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger