[Research of the expression of PTEN in squamous cell carcinoma of larynx by the tissue chip technology].
OBJECTIVE
To evaluate the expression of PTEN in squamous cell carcinoma of larynx and its relationship with factors like pathologic fractionation, clinical TNM stage, and prognosis by the tissue chip technology.


METHODS
We studied the expression of PTEN gene and its mRNA on a series of 146 cases of primary laryngeal carcinoma patients, 40 cases of precancerous lesion and 26 cases of vocal fold polyp by tissue chip by, immunohistochemistry and in situ hybridization method. The observed data observed and some relevant clinical dada were statistically analyzed.


RESULTS
The expression of PTEN in vocal fold polyp was negative, and its positive expression in precancerous lesion and laryngeal carcinoma were 40% and 43.15% respectively by immunohistochemistry, and were 72.50 and 59.59% respectively in situ hybridization. The difference between the expression of PTEN in laryngeal carcinoma and its pathological fraction and prognosis was statistically significant, but was not significant between that and location, clinical stage and LN metastasis. The mRNA expression of PTEN was higher than that of the protein expression in precancerous lesion and cancer tissue.


CONCLUSIONS
The tissue microarray technique required shorter time and less expense, and showed higher consistency in our essays. And the present study suggests PTEN was a prognosis factor of the Laryngeal carcinoma.
Wang
Sang
S
ENT Department, Second Military Medical University, Shanghai, 200433, China.
Liu
Feng
F
Li
Xiaoyan
X
Dong
Pin
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D007830:Larynx / Q000378:metabolism / Q000473:pathology; D008297:Male; D046208:Microchip Analytical Procedures; D008875:Middle Aged; D009367:Neoplasm Staging; D051059:PTEN Phosphohydrolase / Q000378:metabolism*
D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human