title: A method for the rapid isolation of virus from cultured cells.
abstract: A simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cells compared to the widely used freeze-thaw (F-T) method (30 min vs. 3-4 h). This method was also more effective for virion collection. The total number of virions recovered from AMPV-infected immortal turkey turbinate cells by the novel water lysis method was 3-fold higher than by the F-T method. This simple water lysis method can be applied to virion collection for other RNA viruses such as the paramyxoviruses that are used to infect cultured cells.
authors.affiliation: Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, USA.
authors.forename: Byung-Whi
authors.initials: BW
authors.lastname: Kong
authors.forename: Linda K
authors.initials: LK
authors.lastname: Foster
authors.forename: Douglas N
authors.initials: DN
authors.lastname: Foster
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D005615:Freezing; D007038:Hypotonic Solutions; D029121:Metapneumovirus / Q000302:isolation & purification*; D014709:Vero Cells; D014771:Virion / Q000302:isolation & purification; D014867:Water
chemical_list: D007038:Hypotonic Solutions; D014867:Water