title: Agarose gel electrophoresis.
abstract: Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5- to 25-kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample wells and the gel is run at a voltage and for a time period that will achieve optimal separation; and (3) the gel is stained or, if ethidium bromide has been incorporated into the gel and electrophoresis buffer, visualized directly upon illumination with UV light.
authors.affiliation: Iowa State University, Ames, Iowa, USA.
authors.forename: D
authors.initials: D
authors.lastname: Voytas
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D007202:Indicators and Reagents; D010781:Photography; D012685:Sepharose / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D007202:Indicators and Reagents; D004247:DNA; D012685:Sepharose; D004996:Ethidium