title: Growth and manipulation of S. pombe.
abstract: This unit presents aspects specific to genetic and cytological manipulation of fission yeast, including mating type testing, crosses, preparingmaking diploids, and analysis of meiotic products, and basic methods of cell cycle synchronization and analysis. These methods are different from those used in budding yeast because they depend upon the distinct biology of S. pombe, particularly its unwillingness to be a diploid in normal growth conditions. Similarly, the different cell shape and growth behavior of S. pombe require different approaches to basic cell cycle analysis.
authors.affiliation: The Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Susan L
authors.initials: SL
authors.lastname: Forsburg
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002499:Centrifugation, Density Gradient / Q000379:methods; D003433:Crosses, Genetic; D003470:Culture Media / Q000494:pharmacology; D004171:Diploidy; D007202:Indicators and Reagents; D007785:Lactose; D009172:Mycology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000254:growth & development*; D013172:Spores, Fungal; D016707:Tissue Fixation / Q000379:methods
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D007785:Lactose; D009584:Nitrogen