title: Human CyP33 binds specifically to mRNA and binding stimulates PPIase activity of hCyP33.
abstract: Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity of hCyP33 to different cellular RNA using ion-exchange chromatography and affinity adsorption. Furthermore, the influence of different cellular RNAs to the PPIase activity of hCyP33 was investigated using a protease-coupled method. The results show that hCyP33 binds specifically to mRNA, namely poly(A)(+)RNA, and that binding stimulates the PPIase activity of hCyP33.
authors.affiliation: Biochemical Section of Key Laboratory of Functional Polymer Materials, The Ministry of Education of China, Institute of Polymer Chemistry, Chemical School of Nankai University, Tianjin, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruifang
authors.initials: R
authors.lastname: Han
authors.forename: Wanqi
authors.initials: W
authors.lastname: Zhang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yuan
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Long
authors.forename: Huaifeng
authors.initials: H
authors.lastname: Mi
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002852:Chromatography, Ion Exchange; D021983:Cyclophilins / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013552:Swine
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D016572:Cyclosporine; D021983:Cyclophilins; C114522:PPIE protein, human; D019696:Peptidylprolyl Isomerase