title: Analysis of disaccharides and tetrasaccharides released from glycosaminoglycans.
abstract: Glycosaminoglycans (GAGs) are converted to disaccharides by various methods. This unit describes separation of individual disaccharides by paper chromatography or paper electrophoresis or high-performance liquid chromatography (HPLC). Lyase-released disaccharides can also be monitored by UV absorbance. Support protocols describe mild conditions for reduction of alkali-labile disaccharides obtained by cleavage of GAGs with lyases, scintillation counting of samples obtained from HPLC separation of radiolabeled saccharides, and calculations for the quantitation of disaccharides.
authors.affiliation: University of Illinois, Urbana, Illinois, USA.
authors.forename: H E
authors.initials: HE
authors.lastname: Conrad
mesh_terms: D000468:Alkalies; D001894:Borohydrides; D002845:Chromatography; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D009844:Oligosaccharides / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D000468:Alkalies; D001894:Borohydrides; D004187:Disaccharides; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D008190:Lyases