Effect of antibiotics, alone and in combination, on Panton-Valentine leukocidin production by a Staphylococcus aureus reference strain.
The capacity of Staphylococcus aureus strain LUG855 to release Panton-Valentine leukocidin (PVL) in the presence of sub-inhibitory concentrations of anti-staphylococcal drugs was examined. Oxacillin enhanced PVL release 2.5-fold, while clindamycin, linezolid, fusidic acid and rifampicin were inhibitory, and vancomycin, pristinamycin, tetracycline, ofloxacin and co-trimoxazole had no effect. In combination with oxacillin, sub-inhibitory concentrations of clindamycin or rifampicin inhibited PVL induction significantly, linezolid was less inhibitory, and fusidic acid did not inhibit PVL induction by oxacillin. These data support the use of oxacillin in combination with clindamycin, rifampicin or linezolid for the treatment of PVL-positive S. aureus infections.
Dumitrescu
O
O
INSERM U851 and Université Lyon 1, Centre National de Référence des Staphylocoques, Faculté Laennec, Lyon, France.
Badiou
C
C
Bes
M
M
Reverdy
M-E
ME
Vandenesch
F
F
Etienne
J
J
Lina
G
G
D000081:Acetamides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D002981:Clindamycin / Q000494:pharmacology; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005098:Exotoxins / Q000096:biosynthesis* / Q000235:genetics; D005672:Fusidic Acid / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007956:Leukocidins / Q000096:biosynthesis* / Q000235:genetics; D000069349:Linezolid; D008826:Microbial Sensitivity Tests / Q000379:methods; D010068:Oxacillin / Q000494:pharmacology; D023303:Oxazolidinones / Q000494:pharmacology; D012015:Reference Standards; D012293:Rifampin / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
D000081:Acetamides; D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; D023303:Oxazolidinones; C078284:Panton-Valentine leukocidin; D002981:Clindamycin; D005672:Fusidic Acid; D000069349:Linezolid; D010068:Oxacillin; D012293:Rifampin