Excretion of catecholamines in rats, mice and chicken.
Stress assessment favours methods, which do not interfere with an animal's endocrine status. To develop such non-invasive methods, detailed knowledge about the excretion of hormone metabolites in the faeces and urine is necessary. Our study was therefore designed to generate basic information about catecholamine excretion in rats, mice and chickens. After administration of (3)H-epinephrine or (3)H-norepinephrine to male and female rats, mice and chickens, all voided excreta were collected for 4 weeks, 3 weeks or for 10 days, respectively. Peak concentrations of radioactivity appeared in one of the first urinary samples of mice and rats and in the first droppings in chickens 0.2-7.2 h after injection. In rats, between 77.3 and 95.6% of the recovered catecholamine metabolites were found in the urine, while in mice, a mean of 76.3% were excreted in the urine. Peak concentrations in the faeces were found 7.4 h post injection in mice, and after about 16.4 h in rats (means). Our study provides valuable data about the route and the profile of catecholamine excretion in three frequently used species of laboratory animals. This represents the first step in the development of a reliable, non-invasive quantification of epinephrine and norepinephrine to monitor sympatho-adrenomedullary activity, although promising results for the development of a non-invasive method were found only for the chicken.
Lepschy
M
M
Institute of Biochemistry, Department of Natural Sciences, University of Veterinary Medicine, 1210 Vienna, Austria. michael.lepschy@vu-wien.ac.at
Rettenbacher
S
S
Touma
C
C
Palme
R G
RG
D000818:Animals; D002395:Catecholamines / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine*; D002645:Chickens; D004837:Epinephrine / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine; D005243:Feces / Q000737:chemistry*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine; D051381:Rats; D011916:Rats, Inbred F344; D012737:Sex Factors; D013045:Species Specificity; D014316:Tritium
D002395:Catecholamines; D014316:Tritium; D009638:Norepinephrine; D004837:Epinephrine