Beta-elimination for release of O-GalNAc-linked oligosaccharides from glycoproteins and glycopeptides.
This unit describes release of oligosaccharides that are attached to polypeptides through an N-acetylgalactosamine (GalNAc) linkage to the hydroxyl groups of serine or threonine. The beta-elimination procedures described here can be used to recover the oligosaccharide chains (also called glycans) and/or identify the serine or threonine residues involved in the linkage. A beta-elimination method employing sodium borohydride (NaBH4) and alkaline conditions is described, and an alternative method is also presented in which only alkaline conditions are used without a reducing agent. Another alternative protocol uses sodium sulfite.
Fukuda
M
M
La Jolla Cancer Research Foundation, La Jolla, California, USA.
D000818:Animals; D001894:Borohydrides / Q000737:chemistry; D002854:Chromatography, Paper; D004121:Dimethyl Sulfoxide; D005690:Galactose / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D012996:Solutions; D013997:Time Factors
D001894:Borohydrides; D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D012996:Solutions; C025364:sodium borohydride; D005690:Galactose; D004121:Dimethyl Sulfoxide