title: Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth.
abstract: Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by chi(2) test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m(2) (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P = .02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.
authors.affiliation: Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.lastname: Guoyang Luo
authors.forename: Thomas
authors.initials: T
authors.lastname: Morgan
authors.forename: Mert O
authors.initials: MO
authors.lastname: Bahtiyar
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Snegovskikh
authors.forename: Frederick
authors.initials: F
authors.lastname: Schatz
authors.forename: Edward
authors.initials: E
authors.lastname: Kuczynski
authors.forename: Edmund F
authors.initials: EF
authors.lastname: Funai
authors.forename: Antonette T
authors.initials: AT
authors.lastname: Dulay
authors.forename: Se-Te Joseph
authors.initials: ST
authors.lastname: Huang
authors.forename: Catalin S
authors.initials: CS
authors.lastname: Buhimschi
authors.forename: Irina A
authors.initials: IA
authors.lastname: Buhimschi
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Fortunato
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Menon
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Lockwood
authors.forename: Errol R
authors.initials: ER
authors.lastname: Norwitz
mesh_terms: D000328:Adult; D005260:Female; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D047928:Premature Birth / Q000235:genetics*; D011980:Receptors, Progesterone / Q000235:genetics*
chemical_list: D011980:Receptors, Progesterone