Growing lambda-derived vectors.
It is often necessary to grow large quantities of lambda-derived vectors, so that DNA can be made from them. This unit describes a procedure for making a phage stock by plate lysis, and an Alternate Protocol gives details on how to make a liquid lysate. Storage of phage lysates is described in a Support Protocol.
Lech
K
K
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
D010582:Bacteriophage lambda / Q000254:growth & development*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000821:virology*; D005822:Genetic Vectors; D008967:Molecular Biology / Q000379:methods*