Trf4 is a useful gene for discrimination of Candida tropicalis from other medically important Candida species.
Comparative studies of random amplified polymorphic DNA (RAPD) band patterns of Candida tropicalis with those of clinically important Candida species have shown the presence of specific RAPD bands for C. tropicalis. A band specific to C. tropicalis strains (ca. 400 bp) was extracted and sequenced. It was found to belong to a fragment of the Trf4 gene, which is essential for growth of these strains and has a characteristic sequence of C. tropicalis. A PCR primer was designed specifically for C. tropicalis which amplifies the 324 bp band. The PCR primer amplified DNA products for all C. tropicalis strains tested, but did not amplify any PCR bands from C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, C. kefer, C. krusei, C. parapsilosis, or C. zeylanoides. Usefulness of the PCR primer in differentiating from clinical isolates of other fungal species is discussed.
Kang
Yingqian
Y
Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Japan.
Iida
Soji
S
Yamamoto
Setsuya
S
Kogure
Takahisa
T
Tanaka
Reiko
R
Mikami
Yuzuru
Y
D041022:Candida tropicalis / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D005800:Genes, Fungal / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D004259:DNA-Directed DNA Polymerase; C487545:PAP2 protein, S cerevisiae