title: In vitro propagation and chemical and biological studies of the essential oil of Salvia przewalskii Maxim.
abstract: The procedure of Salvia przewalskii shoot multiplication and the ability of regenerated plants to produce essential oil is reported. The essential oil was obtained by hydrodistillation from leaves and flowering stems of field-grown plants, and their chemical composition was examined by GC, GC-MS and 1H NMR. The differences in yield as well as qualitative and quantitative composition between the oils isolated from in vitro and in vivo plants were observed. S. przewalskii essential oil was tested for its antimicrobial and cytotoxic properties. It was found that cytotoxicity against human leukemia HL-60 cells and antimicrobial activity (especially, against Staphylococcus aureus and S. epidermidis strains) of oils isolated from in vitro plants were higher than those for oils from in vivo S. przewalskii plants.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Łódz, Poland. botanika@pharm.am.lodz.pl
authors.forename: Ewa
authors.initials: E
authors.lastname: Skala
authors.forename: Danuta
authors.initials: D
authors.lastname: Kalemba
authors.forename: Anna
authors.initials: A
authors.lastname: Wajs
authors.forename: Marek
authors.initials: M
authors.lastname: Rózalski
authors.forename: Urszula
authors.initials: U
authors.lastname: Krajewska
authors.forename: Barbara
authors.initials: B
authors.lastname: Rózalska
authors.forename: Marzena
authors.initials: M
authors.lastname: Wieckowska-Szakiel
authors.forename: Halina
authors.initials: H
authors.lastname: Wysokińska
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002849:Chromatography, Gas; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000302:isolation & purification* / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D009822:Oils, Volatile; D010938:Plant Oils