Characterization of B/C recombinants of near full-length HIV type 1 from northeastern India with mosaics identical to ARE195FL but with a different ancestral origin.
We have characterized near full-length genomes of three B/C recombinants of HIV-1 from the northeastern state of India. The recombinant viruses showed a backbone of subtype C virus with a single insertion of the subtype B genome in the envelope region. While all of them were distinct from B/C recombinants CRF_07 and CRF_08 circulating in China and CRF_04BR137 circulating in Brazil, two of them presented with break-points identical to the Argentinean B/C recombinant ARE195FL. However, neighbor-joining analysis followed by phylogenetic clustering showed that gp120 belonging to subtype B of all the recombinants clustered with Thai B sequences, while subtype C gag clustered with an Indian subtype C sequence, suggesting a unique ancestral origin of these recombinants.
Lakhashe
Samir
S
Division of Molecular Virology, National AIDS Research Institute, Indian Council of Medical Research, Bhosari, Pune-411026, India.
Tripathy
Srikanth
S
Paranjape
Ramesh
R
Bhattacharya
Jayanta
J
D019143:Evolution, Molecular; D015683:Gene Products, gag / Q000235:genetics*; D016679:Genome, Viral; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D009030:Mosaicism; D010802:Phylogeny; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
D015683:Gene Products, gag; D015699:HIV Envelope Protein gp120