[Simvastatin downregulates CD40L induced vascular cell adhesion molecule-1 expression and adhesive function in human umbilical vein endothelial cells].
OBJECTIVE
To investigate the effects of simvastatin on vascular cell adhesion molecule-1 (VCAM-1) expression and adhesive function in ECV-304 cells treated with CD40L.


METHODS
Human umbilical vein endothelial cell (HUVEC) was cultured and treated with various concentrations CD40L alone or in combination with various concentrations simvastatin in the absence or presence of mevalonic acid (400 micromol/L). RT-PCR and FCM analysis were used to determine VCAM-1 expression and lymphocytes adhesion to endothelial cells.


RESULTS
Simvastatin (0 - 10 micromol/L) decreased in a concentration-dependent manner the expression of VCAM-1 induced by CD40L and this effect could be blocked by cotreatment with mevalonic acid. Moreover, Simvastatin also significantly decreased adhesion capacity of lymphocytes to endothelial cells induced by CD40L.


CONCLUSIONS
Simvastatin downregulates VCAM-1 expression and adhesive capacity of lymphocytes to endothelial cells induced by CD40L.
Zhang
Min
M
Department of Cardiology, Chest Hospital, Shanghai Jiaotong University, Shanghai 200030, China.
Fang
Wei-Yi
WY
Yuan
Fang
F
D023201:CD40 Ligand / Q000378:metabolism*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004727:Endothelium / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D019821:Simvastatin / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D023201:CD40 Ligand; D019821:Simvastatin