Evaluation of the Premi Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica.
A new commercial system based on genetic profiling and aimed at identifying Salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. Within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. Results were obtained within 8 h, were reproducible and clear-cut. The system allowed single-tube processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing.
Wattiau
Pierre
P
VAR (Veterinary and Agrochemical Research Centre) Department of Bacteriology and Immunology, Groeselenberg 99, B-1180 Brussels, Belgium. Pierre.Wattiau@var.fgov.be
Weijers
Thijs
T
Andreoli
Peter
P
Schliker
Christine
C
Veken
Heidi Vander
HV
Maas
Henny M E
HM
Verbruggen
Anjo J
AJ
Heck
Max E O C
ME
Wannet
Wim J
WJ
Imberechts
Hein
H
Vos
Pieter
P
D015373:Bacterial Typing Techniques / Q000379:methods; D003257:Consumer Product Safety; D004269:DNA, Bacterial / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D020869:Gene Expression Profiling; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D019779:Salmonella enterica / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013045:Species Specificity
D004269:DNA, Bacterial