Single nucleotide polymorphisms in the human progesterone receptor gene and spontaneous preterm birth.
Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by chi(2) test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m(2) (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P = .02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.
Guoyang Luo
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut, USA.
Morgan
Thomas
T
Bahtiyar
Mert O
MO
Snegovskikh
Victoria V
VV
Schatz
Frederick
F
Kuczynski
Edward
E
Funai
Edmund F
EF
Dulay
Antonette T
AT
Huang
Se-Te Joseph
ST
Buhimschi
Catalin S
CS
Buhimschi
Irina A
IA
Fortunato
Stephen J
SJ
Menon
Ramkumar
R
Lockwood
Charles J
CJ
Norwitz
Errol R
ER
D000328:Adult; D005260:Female; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D047928:Premature Birth / Q000235:genetics*; D011980:Receptors, Progesterone / Q000235:genetics*
D011980:Receptors, Progesterone