title: Cortactin expression predicts poor survival in laryngeal carcinoma.
abstract: Amplification of the 11q13 region is one of the most frequent aberrations in squamous cell carcinomas of the head and neck region (HNSCC). Amplification of 11q13 has been shown to correlate with the presence of lymph node metastases and decreased survival. The 11q13.3 amplicon carries numerous genes including cyclin D1 and cortactin. Recently, we reported that FADD becomes overexpressed upon amplification and that FADD protein expression predicts for lymph node positivity and disease-specific mortality. However, the gene within the 11q13.3 amplicon responsible for this correlation is yet to be identified. In this paper, we compared, using immunohistochemical analysis for cyclin D1, FADD and cortactin in a series of 106 laryngeal carcinomas which gene correlates best with lymph node metastases and increased disease-specific mortality. Univariate Cox regression analysis revealed that high expression of cyclin D1 (P=0.016), FADD (P=0.003) and cortactin (P=0.0006) predict for increased risk to disease-specific mortality. Multivariate Cox analysis revealed that only high cortactin expression correlates with disease-specific mortality independent of cyclin D1 and/or FADD. Of genes located in the 11q13 amplicon, cortactin expression is the best predictor for shorter disease-specific survival in late stage laryngeal carcinomas.
authors.affiliation: Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
authors.forename: J H
authors.initials: JH
authors.lastname: Gibcus
authors.forename: M F
authors.initials: MF
authors.lastname: Mastik
authors.forename: L
authors.initials: L
authors.lastname: Menkema
authors.forename: G H
authors.initials: GH
authors.lastname: de Bock
authors.forename: Ph M
authors.initials: PM
authors.lastname: Kluin
authors.forename: Ed
authors.initials: E
authors.lastname: Schuuring
authors.forename: J E
authors.initials: JE
authors.lastname: van der Wal
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002880:Chromosomes, Human, Pair 11; D051356:Cortactin / Q000032:analysis*; D019938:Cyclin D1 / Q000032:analysis; D053200:Fas-Associated Death Domain Protein / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000737:chemistry / Q000235:genetics / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012044:Regression Analysis
chemical_list: C495868:CTTN protein, human; D051356:Cortactin; C505533:FADD protein, human; D053200:Fas-Associated Death Domain Protein; D019938:Cyclin D1