title: Identification and characterization of novel mammalian spermatogenic genes conserved from fly to human.
abstract: Spermatogenesis is a complex and highly regulated developmental process by which round spermatogonial stem cells undergo mitotic proliferation and meiosis, followed by extraordinary differentiation into highly specialized elongated mature sperm. Extensive differences in terms of sperm production such as testicular structure and organization, hormonal regulation are reported between humans and insects, yet it is not known to what extent components of the process could be conserved and furthermore to what extent the underlying genetic regulators could be shared from insects to mammals. We hence take a genomic approach to identify genes which are expressed in the testes of both fly and mouse through in silico analysis and are phylogenetically conserved across metazoans. Fifty eight testis-enriched, phylogenetically conserved from fly to mouse genes were identified. Among them, 12 genes are novel. Detailed characterization of their murine and human homologs indicate most of them are testis-restricted or enriched and developmentally regulated, thus suggesting that they are important regulators of sperm development in mammals and potential human fertility factors. Our results reveal the existence of spermatogenic homologs with similar testicular expression across a large evolutionary distance, further functional study will be needed to explore the functional conservation among those spermatogenic orthologs.
authors.affiliation: Divison of Reproductive Biology Research, Department of Obstetrics and Gynecology, Center for Genetic Medicine, Northwestern University, Feinberg School of Medicine, Lurie 7-117, Chicago, IL 60611, USA.
authors.forename: Edmundo
authors.initials: E
authors.lastname: Bonilla
authors.forename: Eugene Yujun
authors.initials: EY
authors.lastname: Xu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D004330:Drosophila; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D051379:Mice; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*