title: Homogenous demineralized dentin matrix for application in cranioplasty of rabbits with alloxan-induced diabetes: histomorphometric analysis.
abstract: OBJECTIVE
  The aim of this work was to evaluate the bone-repair process after implantation of homogenous demineralized dentin matrix (HDDM) slices in surgical defects created in the parietal bones of rabbits with alloxan-induced diabetes.
  
  
  METHODS
  Forty-eight rabbits were selected and divided into 4 groups of 12 rabbits: the control group, diabetic rabbits (D), diabetic rabbits with a PTFE barrier (D-PTFE), and diabetic rabbits with a PTFE barrier and with slices of homogenous demineralized dentin matrix (D-PTFE+HDDM). The diabetic animals received a single dose of alloxan monohydrate (90 mg/kg) intravenously on the marginal ear vein, and their blood glucose was verified daily. The rabbits were sacrificed after 15, 30, 60, and 90 days. The histologic findings show both better bone structure and significantly greater bone density, as determined by histomorphometric analysis, for the D-PTFE + HDDM group than for the other 3 groups (P < .01). It was also observed that the mean bone density increased gradually from 15 to 90 days (except in the D-PTFE group).
  
  
  CONCLUSIONS
  It was concluded that the HDDM was biocompatible with the bone repair of diabetic rabbits and that HDDM slices stimulated bone tissue formation. Facilitation of bone repair with HDDM could be useful in diabetic patients.
authors.affiliation: Department of Biosciences and Oral Diagnosis, São José dos Campos Dental School, São Paulo State University, Brazil. mfgomes@fosjc.unesp.br
authors.forename: Mônica Fernandes
authors.initials: MF
authors.lastname: Gomes
authors.forename: Efani Caroline de Freitas
authors.initials: EC
authors.lastname: Banzi
authors.forename: Maria Fernanda de Souza Setúbal
authors.initials: MF
authors.lastname: Destro
authors.forename: Valmir
authors.initials: V
authors.lastname: Lavinicki
authors.forename: Maria das Graças Vilela
authors.initials: Md
authors.lastname: Goulart
mesh_terms: D000496:Alloxan; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism / Q000627:therapeutic use; D001843:Bone Cements / Q000378:metabolism*; D001861:Bone Regeneration / Q000502:physiology*; D003804:Dentin / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000601:surgery; D005500:Follow-Up Studies; D048091:Guided Tissue Regeneration / Q000379:methods*; D008567:Membranes, Artificial; D010294:Parietal Bone / Q000378:metabolism* / Q000601:surgery; D011138:Polytetrafluoroethylene / Q000627:therapeutic use; D011817:Rabbits; D014945:Wound Healing / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; C093073:Demineralized Dentin Matrix; D008567:Membranes, Artificial; D000496:Alloxan; D011138:Polytetrafluoroethylene