Amplification of a bacteriophage library.
This protocol may be used for genomic DNA or cDNA libraries. A freshly packaged and titered library is adsorbed to log phase plating bacteria. The mixture is then plated at high density and allowed to grow until the plaques are just subconfluent. The phage are eluted from the plate by overnight incubation with phage buffer and the library is titered and stored.
Klickstein
L B
LB
Brigham and Women's Hospital, Boston, Massachusetts, USA.
D001435:Bacteriophages / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification
D018076:DNA, Complementary; D004247:DNA