title: Thrombopoietin inhibits murine mast cell differentiation.
abstract: We have recently shown that Mpl, the thrombopoietin receptor, is expressed on murine mast cells and on their precursors and that targeted deletion of the Mpl gene increases mast cell differentiation in mice. Here we report that treatment of mice with thrombopoietin or addition of this growth factor to bone marrow-derived mast cell cultures severely hampers the generation of mature cells from their precursors by inducing apoptosis. Analysis of the expression profiling of mast cells obtained in the presence of thrombopoietin suggests that thrombopoietin induces apoptosis of mast cells by reducing expression of the transcription factor Mitf and its target antiapoptotic gene Bcl2.
authors.affiliation: Hematology, Oncology and Molecular Medicine, Istituto Superiore Sanità, Rome, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Martelli
authors.forename: Barbara
authors.initials: B
authors.lastname: Ghinassi
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Lorenzini
authors.forename: Alessandro M
authors.initials: AM
authors.lastname: Vannucchi
authors.forename: Rosa Alba
authors.initials: RA
authors.lastname: Rana
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Sandra
authors.initials: S
authors.lastname: Partamian
authors.forename: Giovanni
authors.initials: G
authors.lastname: Migliaccio
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Migliaccio
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D013926:Thrombopoietin / Q000502:physiology*
chemical_list: D013926:Thrombopoietin