title: Gene deletion and allelic replacement in the filamentous fungus Podospora anserina.
abstract: Gene replacement via homologous recombination is a fundamental tool for the analysis of gene function. However, this event is rare in organisms like the filamentous fungus Podospora anserina. We show here that deletion of the PaKu70 gene is an efficient strategy for improving gene manipulation in this organism. By using the DeltaPaKu70 strain, it is now possible (1) to produce deletion mutants with an efficiency of 100%, (2) to achieve allelic exchange by introducing a mutated allele associated with a selection cassette at the locus, (3) to introduce a mutation in a gene without co-insertion of a selectable marker and without any modification of the target locus.
authors.affiliation: CNRS, Centre de Génétique Moléculaire, UPR 2167, Avenue de la Terrasse, Bat. 26, 91198, Gif-sur-Yvette, France.
authors.forename: Riyad
authors.initials: R
authors.lastname: El-Khoury
authors.forename: Carole H
authors.initials: CH
authors.lastname: Sellem
authors.forename: Evelyne
authors.initials: E
authors.lastname: Coppin
authors.forename: Antoine
authors.initials: A
authors.lastname: Boivin
authors.forename: Marc F P M
authors.initials: MF
authors.lastname: Maas
authors.forename: Robert
authors.initials: R
authors.lastname: Debuchy
authors.forename: Annie
authors.initials: A
authors.lastname: Sainsard-Chanet
mesh_terms: D000483:Alleles; D034961:Antigens, Nuclear / Q000235:genetics; D015139:Blotting, Southern; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D017353:Gene Deletion; D018390:Gene Targeting; D005800:Genes, Fungal / Q000235:genetics*; D000072200:Ku Autoantigen; D048468:Podospora / Q000235:genetics*
chemical_list: D034961:Antigens, Nuclear; D004268:DNA-Binding Proteins; D000072200:Ku Autoantigen