Ultraviolet resonance Raman microprobe spectroscopy of photosystem II.
Photosystem II (PSII) carries out photosynthetic oxygen production and is responsible for the maintenance of aerobic, heterotrophic life. In PSII, protein amino acid residues play an important role in the light-driven electron transfer reactions. Here, we describe an approach to enhancing vibrational signals from PSII proteins through ultraviolet resonance Raman (UVRR) and a microprobe jet flow technique. Our work shows that pump-probe UVRR can be used to monitor intermediates during photosynthetic oxygen evolution.
Chen
Jun
J
School of Chemistry and Biochemistry and the Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA.
Barry
Bridgette A
BA
D045332:Photosystem II Protein Complex / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods
D045332:Photosystem II Protein Complex