title: Imprinting effect of protein-imprinted polymers composed of chitosan and polyacrylamide: a re-examination.
abstract: In this paper the imprinting effects recently reported with protein-imprinted polymers based on chitosan and polyacrylamide are re-assessed. Molecularly imprinted polymers (MIPs) from which the embedded template molecules were removed by washing with a solution of sodium dodecyl sulfate and acetic acid were prepared. In batch template rebinding experiments, the MIPs displayed quite high template binding capacity as reported previously. However, the non-imprinted polymers (NIPs), after washing with the same solution, also showed large binding capacity nearly equal to that of the MIPs. X-ray diffraction and scanning electronic microscope investigations confirmed remarkable property changes of the NIPs after the washing process. These findings indicate that the non-specific adsorption resulting from the template removing process rather than the imprinted sites generated on the MIPs themselves may account for the high template rebinding capacity of the reported protein-imprinted polymers.
authors.affiliation: Key Laboratory of Functional Polymers of Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, PR China. gqfu@nankai.edu.cn <gqfu@nankai.edu.cn>
authors.forename: Guo-Qi
authors.initials: GQ
authors.lastname: Fu
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D012709:Serum Albumin / Q000737:chemistry* / Q000648:ultrastructure; D014961:X-Ray Diffraction; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D048271:Chitosan; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D012709:Serum Albumin; D000099166:Polyacrylamides