Gene silencing by RNAi in mammalian cells.
This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways for siRNA generation and optimisation, as well as recommendations for cell transfection are presented.
John
Matthias
M
Ribopharma AG, Kulmbach, Germany.
Geick
Anke
A
Hadwiger
Philipp
P
Vornlocher
Hans-Peter
HP
Heidenreich
Olaf
O
D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D017930:Genes, Reporter; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D008081:Liposomes; D008322:Mammals / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D034741:RNA, Small Interfering