title: Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1.
abstract: Brucella is an intracellular pathogen able to persist for long periods of time within the host and establish a chronic disease. We show that soon after Brucella inoculation in intestinal loops, dendritic cells from ileal Peyer's patches become infected and constitute a cell target for this pathogen. In vitro, we found that Brucella replicates within dendritic cells and hinders their functional activation. In addition, we identified a new Brucella protein Btp1, which down-modulates maturation of infected dendritic cells by interfering with the TLR2 signaling pathway. These results show that intracellular Brucella is able to control dendritic cell function, which may have important consequences in the development of chronic brucellosis.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Faculté de Sciences de Luminy, Marseille, France.
authors.forename: Suzana P
authors.initials: SP
authors.lastname: Salcedo
authors.forename: María Ines
authors.initials: MI
authors.lastname: Marchesini
authors.forename: Hugues
authors.initials: H
authors.lastname: Lelouard
authors.forename: Emilie
authors.initials: E
authors.lastname: Fugier
authors.forename: Gilles
authors.initials: G
authors.lastname: Jolly
authors.forename: Stephanie
authors.initials: S
authors.lastname: Balor
authors.forename: Alexandre
authors.initials: A
authors.lastname: Muller
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lapaque
authors.forename: Olivier
authors.initials: O
authors.lastname: Demaria
authors.forename: Lena
authors.initials: L
authors.lastname: Alexopoulou
authors.forename: Diego J
authors.initials: DJ
authors.lastname: Comerci
authors.forename: Rodolfo A
authors.initials: RA
authors.lastname: Ugalde
authors.forename: Philippe
authors.initials: P
authors.lastname: Pierre
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Gorvel
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D004195:Disease Models, Animal; D015536:Down-Regulation; D054884:Host-Pathogen Interactions; D007082:Ileum / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010581:Peyer's Patches / Q000382:microbiology; D051195:Toll-Like Receptor 2 / Q000378:metabolism*
chemical_list: D016207:Cytokines; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2