title: [Bactericidal activity of GLL-37, a novel derivative of the human antimicrobial peptide LL-37].
abstract: To develop and investigate GLL-37, a substitution analogue of the human antimicrobial peptide LL-37 with anti-enzymatic degradation activity and improved efficacy.
  
  
  
  The bactericidal activities of LL-37 and newly developed GLL-37 against 6 Gram-negative and -positive bacteria were determined by Broth microdilution assays. The minimum inhibitory concentrations of LL-37 and GLL-37 against E.coli ATCC 25922 in different NaCl concentration medium were also detected. Both peptides were co-incubated with elastase, and then analyzed by PAGE electrophoresis and bactericidal activity determination.
  
  
  
  GLL-37 showed a stronger elastase resistance ability than LL-37, and was significantly more effective than LL-37 under high-salt condition.
  
  
  
  The antimicrobial peptide GLL-37 derived form LL-37 has the potential as a new therapeutic agent for bacterial infections.
authors.affiliation: Department of Immunology and Microbiology, Xioan Jiaotong University School of Medinice, Xioan 710061, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shi
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Gui-xiang
authors.initials: GX
authors.lastname: Fan
authors.forename: Yu-kang
authors.initials: YK
authors.lastname: Yuan
authors.forename: Long
authors.initials: L
authors.lastname: Mei
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology* / Q000627:therapeutic use; D001770:Blood Bactericidal Activity / Q000187:drug effects*; D054804:Cathelicidins; D002463:Cell Membrane Permeability / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects; D011552:Pseudomonas Infections / Q000188:drug therapy
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D008565:Membrane Proteins