In situ delivery of bone marrow cells and mesenchymal stem cells improves cardiovascular function in hypertensive rats submitted to myocardial infarction.
This work aimed to evaluate cardiac morphology/function and histological changes induced by bone marrow cells (BMCs) and cultured mesenchymal stem cells (MSCs) injected at the myocardium of spontaneously hypertensive rats (SHR) submitted to surgical coronary occlusion. Female syngeneic adult SHR, submitted (MI) or not (C) to coronary occlusion, were treated 24 h later with in situ injections of normal medium (NM), or with MSCs (MSC) or BMCs (BM) from male rats. The animals were evaluated after 1 and 30 days by echocardiography, histology of heart sections and PCR for the Y chromosome. Improved ejection fraction and reduced left ventricle infarcted area were observed in MSC rats as compared to the other experimental groups. Treated groups had significantly reduced lesion tissue score, increased capillary density and normal (not-atrophied) myocytes, as compared to NM and C groups. The survival rate was higher in C, NM and MSC groups as compared to MI and BM groups. In situ injection of both MSCs and BMCs resulted in improved cardiac morphology, in a more physiological model of myocardial infarction represented by surgical coronary occlusion of spontaneously hypertensive rats. Only treatment with MSCs, however, ameliorated left ventricle dysfunction, suggesting a positive role of these cells in heart remodeling in infarcted hypertensive subjects.
de Macedo Braga
Luisa Maria Gomes
LM
Department of Genetics, Universidade Federal do Rio Grande do Sul, Av Bento Goncalves 9500, 91501-970, Porto Alegre, RS, Brazil.
Lacchini
Silvia
S
Schaan
Beatriz D'Agord
BD
Rodrigues
Bruno
B
Rosa
Kaleizu
K
De Angelis
Kátia
K
Borges
Luciano Figueiredo
LF
Irigoyen
Maria Cláudia
MC
Nardi
Nance Beyer
NB
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology*; D002319:Cardiovascular System / Q000503:physiopathology; D017690:Cell Transplantation; D017931:DNA Primers; D004195:Disease Models, Animal; D004452:Echocardiography; D005260:Female; D006973:Hypertension / Q000601:surgery*; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000601:surgery*; D016133:Polymerase Chain Reaction; D051381:Rats; D011918:Rats, Inbred SHR
D017931:DNA Primers