Production of a complete cDNA library.
A complete cDNA library is one that contains at least one cDNA clone representing each mRNA in the cell. A method is provided in this unit for calculating the required base of a cDNA library such that the library should contain at least one copy of every mRNA. Either a phage or plasmid vector can be used in constructing a complete cDNA library, and protocols are described for both. Procedures for evaluating these cDNA libraries are also provided along with a procedure for evaluating the quality of the lambda arms used in constructing complete phage cDNA libraries.
Klickstein
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LB
Brigham and Women's Hospital, Boston, Massachusetts, USA.
D010582:Bacteriophage lambda / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger