Luteolytic effect of prostaglandin F 2 alpha on bovine corpus luteum depends on cell composition and contact.
Prostaglandin F(2 alpha) (PGF(2 alpha)) is a main luteolytic factor in vivo; however, its direct luteolytic influence on steroidogenic cells of bovine corpus luteum (CL) is controversial and not fully understood. The aim of the study was to clarify PGF(2 alpha) action on bovine CL in different in vivo and in vitro conditions and to examine whether the contact among all main types of CL cells is necessary for luteolytic PGF(2 alpha) action. In experiment 1, the bovine CL (day 15 of the oestrous cycle) was perfused using in vivo microdialysis system with dinoprost (an analogue of PGF(2 alpha)) for 0.5 h. Dinoprost caused a short-time increase in progesterone (P4), whose concentration decreased thereafter (at 6-, 10-, 12- and 24-h after treatment). In experiment 2, the direct effect of PGF(2 alpha) on P4 accumulation in CL steroidogenic cells cultured in monolayer (day 15 of the cycle) was determined. PGF(2 alpha) after 24 h of incubation increased P4 accumulation in steroidogenic CL cells. In experiment 3 steroidogenic, endothelial CL and immune cells (day 15 of the cycle) were incubated with PGF(2 alpha) in cocultures for 24 h in glass tubes and the levels of P4, stable metabolites of nitric oxide (NO) and leukotriene (LT) C(4) were determined. Although PGF(2 alpha) treatment increased P4 secretion in homogeneous steroidogenic CL cell culture, the decrease in P4 secretion in cocultures of all types of CL cells was observed. The secretion of NO and LTC(4) increased after the treatment of PGF(2 alpha) both in pure cultures of CL cells and in cocultures. The interactions between endothelial and immune cells with steroidogenic CL cells are needed for luteolytic PGF(2 alpha) action within the bovine CL. Our results indicate that the cell coculture model, including the main types of CL cells, is the most approximate to study PGF(2 alpha) role in vitro.
Korzekwa
A J
AJ
Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research, PAS, Olsztyn, Poland.
Jaroszewski
J J
JJ
Woclawek-Potocka
I
I
Bah
M M
MM
Skarzynski
D J
DJ
D000818:Animals; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000662:veterinary; D003338:Corpus Luteum / Q000187:drug effects*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000494:pharmacology*; D005260:Female; D017997:Leukotriene C4 / Q000378:metabolism; D003341:Luteolysis / Q000502:physiology*; D017551:Microdialysis / Q000662:veterinary; D009569:Nitric Oxide / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*
D017997:Leukotriene C4; D009569:Nitric Oxide; D011374:Progesterone; D015237:Dinoprost