Evaluation of alpha-galactosidase biosynthesis by Streptomyces griseoloalbus in solid-state fermentation using response surface methodology.
OBJECTIVE
The present study was aimed at evaluating the effects of the three crucial factors, galactose concentration, inoculum size and moisture content, on alpha-galactosidase production by the filamentous actinobacterium Streptomyces griseoloalbus in solid-state fermentation.


RESULTS
Central Composite design was adopted to derive a statistical model for the optimization of fermentation conditions. Maximum alpha-galactosidase yield (117 U g(-1) of dry fermented substrate) was obtained when soya bean flour supplemented with 1.5% galactose and with initial moisture content of 40% was inoculated with 1.9 x 10(6) CFU g(-1) initial dry substrate.


CONCLUSIONS
The model was valid and could result in considerably enhanced enzyme yield.


CONCLUSIONS
The results indicated a cost effective method for the production of alpha-galactosidase using soya bean flour. This is the first report on exploitation of the potential of filamentous bacterium for the production of alpha-galactosidase, an enzyme having versatile applications.
Anisha
G S
GS
Biotechnology Division, National Institute for Interdisciplinary Science and Technology (Formerly Regional Research Laboratory), CSIR, Trivandrum, Kerala, India.
Sukumaran
R K
RK
Prema
P
P
D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D005285:Fermentation; D005690:Galactose / Q000378:metabolism; D006813:Humidity; D008954:Models, Biological; D013025:Glycine max / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development*; D000519:alpha-Galactosidase / Q000096:biosynthesis*
D003470:Culture Media; D000519:alpha-Galactosidase; D005690:Galactose