title: Estimation of hydroxymethylfurfural availability in breakfast cereals. Studies in Caco-2 cells.
abstract: The transport and availability of hydroxymethylfurfural (HMF), an intermediary product of the Maillard reaction, was investigated in the Caco-2 cell line after in vitro gastrointestinal digestion. The study was carried out at two levels; (a) an HMF-spiked culture medium, and (b) digested commercial breakfast cereals (BC). In both assays, the higher the amount of HMF offered to the cells, the higher the absolute value of transported HMF. However, HMF availability and transport are not directly proportional to the initial HMF content since HMF is partly retained in the non-soluble fraction after digestion. In addition, HMF is degraded to some extent during the gastrointestinal digestion of both HMF-spiked cell medium and BC. Average HMF availability from three commercial breakfast cereals was 9.1% (4.98-12.99%). Variations in HMF availability may be related to the particular composition of each BC, where fibre could play an important role. On the other hand, possible metabolization into the cell should also be considered.
authors.affiliation: Unidad de Nutrición, Estación Experimental del Zaidín (CSIC), Camino del Jueves, Granada, Spain. cdelgado@eez.csic.es
authors.forename: Cristina
authors.initials: C
authors.lastname: Delgado-Andrade
authors.forename: Isabel
authors.initials: I
authors.lastname: Seiquer
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Navarro
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Morales
mesh_terms: D001693:Biological Transport, Active; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D002523:Edible Grain / Q000737:chemistry*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D011506:Proteins / Q000737:chemistry; D014357:Trypsin
chemical_list: D003470:Culture Media; D011506:Proteins; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D014357:Trypsin