Effects of a combined application of potassium oxalate gel/adhesive agent on dentin permeability in vitro.
OBJECTIVE
To test the effects of sequential application of potassium oxalate gel/adhesive agent on in vitro dentin permeability.


METHODS
Full crown preparations were made in extracted human molars to expose deep coronal dentin. The roots and pulp were removed and the resulting crown segments were connected to a special device (Flodec) to permit the measurement of the permeability of the specimens before and after treatments. Minimum and maximum permeability were recorded after smear layer and phosphoric acid treatment. A new smear layer was created and the permeability measured after the crowns were bonded with Single Bond (3M ESPE), One-Up Bond F (Tokuyama), and AdheSE (Ivoclar Vivadent), either according to manufacturer's instructions or after treating the acid-etched dentin with a 3 wt% potassium oxalate gel. The results were expressed as a percentage of maximum permeability values. Impressions and epoxy resin replicas from the crown segments were produced for SEM examination.


RESULTS
None of the adhesives were able to eliminate the fluid flow through dentin. Two-way ANOVA revealed that the application of potassium oxalate prior to the bonding procedures was the most effective technique in reducingthe dentin permeability (p < 0.05), regardless of the adhesive used. SEM micrographs showed that transudation of dentinal fluid could be identified on the surfaces of all replicas.


CONCLUSIONS
The use of potassium oxalate gel was effective in reducing the permeability of bonded dentin.
de Andrade e Silva
Safira Marques
SM
Department of Restorative Dentistry, University of Campinas, Piracicaba, Brazil.
Marquezini
Luiz
L
Manso
Adriana Pigozzo
AP
Garcia
Fernanda Pimentel
FP
Carrilho
Marcela Rocha de Oliveira
MR
Pashley
David Henry
DH
Tay
Franklin Russel
FR
Carvalho
Ricardo Marins
RM
D000134:Acid Etching, Dental; D000180:Acrylic Resins / Q000737:chemistry; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D003442:Crowns; D001840:Dental Bonding; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D003806:Dentin Permeability / Q000187:drug effects*; D017220:Dentin-Bonding Agents / Q000494:pharmacology*; D019234:Dentinal Fluid / Q000187:drug effects; D005782:Gels; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010070:Oxalates / Q000494:pharmacology*; D010756:Phosphoric Acids / Q000494:pharmacology; D019163:Reducing Agents / Q000494:pharmacology*; D016369:Smear Layer; D013499:Surface Properties; D019231:Tooth Preparation, Prosthodontic
D000180:Acrylic Resins; C489662:AdheSE cement; D017220:Dentin-Bonding Agents; D005782:Gels; D008689:Methacrylates; C446260:One-Up-Bond F; D010070:Oxalates; D010756:Phosphoric Acids; D019163:Reducing Agents; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C030242:phosphoric acid