title: HPLC of peptides and proteins: standard operating conditions.
abstract: The standard operating conditions for the eight basic modes of HPLC are presented in this unit. They include: size-exclusion chromatography (HP-SEC), ion-exchange chromatography (HP-IEX), normal phase chromatography (HP-NPC), hydrophobic interaction chromatography (HP-HIC), reversed-phase chromatography (RP-HPLC), hydrophilic interaction chromatography (HP-HILIC), immobilized metal ion affinity chromatography (HP-IMAC), and biospecific/biomimetic affinity chromatography (HP-BAC). In addition, some subsets of these chromatographic modes, e.g., mixed mode chromatography (HP-MMC), charge transfer chromatography (HP-CTC), or ligand-exchange chromatography (HP-LEC) are described. Procedures for multimodal column switching are also included, as are guidelines for a systematic approach to method development. Example separations help illustrate the procedures. The standard operating conditions for the eight basic modes of HPLC are presented in this unit.
authors.affiliation: Monash University, Victoria, Australia.
authors.forename: R I
authors.initials: RI
authors.lastname: Boysen
authors.forename: M T
authors.initials: MT
authors.lastname: Hearn
mesh_terms: D000818:Animals; D002021:Buffers; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D002852:Chromatography, Ion Exchange; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012107:Research Design; D012492:Salts; D013816:Thermodynamics
chemical_list: D002021:Buffers; D010455:Peptides; D011506:Proteins; D012492:Salts