Ibuprofen metabolite profiling using a combination of SPE/column-trapping and HPLC-micro-coil NMR.
Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC-nuclear magnetic resonance (HPLC-NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold.
Djukovic
Danijel
D
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Appiah-Amponsah
Emmanuel
E
Shanaiah
Narasimhamurthy
N
Gowda
G A Nagana
GA
Henry
Ian
I
Everly
Mike
M
Tobias
Brian
B
Raftery
Daniel
D
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007052:Ibuprofen / Q000378:metabolism* / Q000652:urine; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D013607:Tablets; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D013607:Tablets; D007052:Ibuprofen