title: Identifying chromosomal targets of DNA-binding proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE).
abstract: Sequence Tag Analysis of Genomic Enrichment (STAGE) is a method for experimentally identifying the in vivo chromosomal targets of DNA-binding proteins in any sequenced genome. STAGE generates 21-bp tags derived from DNA isolated by chromatin immunoprecipitation (ChIP; UNIT 21.3). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA-binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA-binding protein in vivo. STAGE can be applied to any sequenced genome to identify targets of DNA-binding proteins without requiring extensive microarray resources.
authors.affiliation: University of Texas at Austin, Austin, Texas, USA.
authors.forename: Jonghwan
authors.initials: J
authors.lastname: Kim
authors.forename: Vishwanath R
authors.initials: VR
authors.lastname: Iyer
mesh_terms: D035843:Biomedical Research / Q000379:methods*; D047369:Chromatin Immunoprecipitation; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D020224:Expressed Sequence Tags; D016678:Genome; D007202:Indicators and Reagents; D016324:Sequence Tagged Sites
chemical_list: D004268:DNA-Binding Proteins; D007202:Indicators and Reagents