title: Simultaneous determination by UPLC-ESI-MS of scoparone, capillarisin, rhein, and emodin in rat urine after oral administration of Yin Chen Hao Tang preparation.
abstract: A simple, sensitive, and validated method was developed for simultaneous determination of scoparone, capillarisin, rhein, and emodin in rat urine by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC-MS). The urinary samples were analyzed on an Acquity UPLC BEH C18 1.7 microm 2.1x50 mm column. Scoparone, capillarisin, rhein, and emodin in rat urine were simultaneously analyzed with good separation. The lower limits of detection were 6.0, 9.0, 7.0, and 3.0 ng/mL, and the lower limits of quantification were 20.0, 33.0, 24.0, and 12.0 ng/mL for scoparone, capillarisin, rhein, and emodin, respectively. The intra- and inter-day precisions (RSD) were less than 9%. The intra- and inter-accuracies were found to be in the range of 94.14-104.54% for scoparone, 101.72-107.34% for capillarisin, 95.24-103.59% for rhein, and 101.32-107.82% for emodin at three concentration levels. The absolute recoveries for scoparone, capillarisin, rhein, and emodin were not less than 77.0%. The developed method has been applied to determine scoparone, capillarisin, rhein, and emodin in rat urine after oral administration of Yin Chen Hao Tang preparation, a traditional Chinese medicine formulation widely used in China for treatment of jaundice and liver disorders.
authors.affiliation: Department of Pharmacognosy, Heilongjiang University of Chinese Medicine, Harbin, China. Fax: 86-451-82110818.
authors.forename: Haitao
authors.initials: H
authors.lastname: Lv
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Sun
authors.forename: Guozheng
authors.initials: G
authors.lastname: Jiao
authors.forename: Dixin
authors.initials: D
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Hongxin
authors.initials: H
authors.lastname: Cao
authors.forename: Guangmei
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000880:Anthraquinones / Q000652:urine*; D002853:Chromatography, Liquid; D002867:Chromones / Q000652:urine*; D003374:Coumarins / Q000652:urine*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000378:metabolism*; D004642:Emodin / Q000652:urine*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000880:Anthraquinones; D002867:Chromones; D003374:Coumarins; D004365:Drugs, Chinese Herbal; C011185:capillarisin; C018145:scoparone; D004642:Emodin; C020491:rhein