Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor.
By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor's resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0x10(2) colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.
Wang
Li-jiang
LJ
Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.
Wu
Chun-sheng
CS
Hu
Zhao-ying
ZY
Zhang
Yuan-fan
YF
Li
Rong
R
Wang
Ping
P
D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015169:Colony Count, Microbial; D003460:Crystallization; D004563:Electrochemistry; D004573:Electrolytes; D004867:Equipment Design; D019453:Escherichia coli O157 / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D010084:Oxidation-Reduction; D011791:Quartz; D012815:Signal Processing, Computer-Assisted; D012984:Software; D013997:Time Factors
D000906:Antibodies; D004573:Electrolytes; D011791:Quartz; D006046:Gold