Studies on the exchange of early pellicle proteins by mucin and whole saliva.
Adsorption of small pellicle proteins statherin or proline-rich protein 1 (PRP1), respectively, and subsequent adsorption of human whole saliva (HWS) or salivary mucin MUC5B, respectively, was studied using ellipsometry and total internal reflectance fluorescence. Differences in elution (using sodium dodecyl sulphate (SDS) solutions) between mixed and single protein films were also investigated. On both hydrophilic and hydrophobized surfaces HWS and MUC5B were found to adsorb to pre-adsorbed layers of statherin and PRP1, respectively. Statherin adsorption on both substrate types showed no or minor exchange by HWS or MUC5B and no change in SDS elution between mixed and single protein films. Small amounts of PRP1 were exchanged by HWS on both surface types and the SDS elutable fractions were similar or larger for mixed films compared to single protein films. PRP1 and MUC5B in sequence showed minor exchange of PRP1 on hydrophilic surfaces, while no exchange could be established on hydrophobized substrates. SDS elutable fractions decreased for PRP1 and MUC5B mixed films compared to single protein films. In conclusion, minor amounts of statherin and PRP1 are exchanged during the time course of the experiments, which indicates that these proteins may to a large extent remain incorporated in the pellicle.
Svendsen
Ida E
IE
Biomedical Laboratory Science and Technology, Faculty of Health and Society, Malmö University, SE-206 05 Malmö, Sweden. Ida.Svendsen@mah.se
Lindh
Liselott
L
Elofsson
Ulla
U
Arnebrant
Thomas
T
D000327:Adsorption; D005453:Fluorescence; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055257:Mucin-5B; D009077:Mucins / Q000737:chemistry*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
C524578:MUC5B protein, human; D055257:Mucin-5B; D009077:Mucins; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D012967:Sodium Dodecyl Sulfate