Imprinting effect of protein-imprinted polymers composed of chitosan and polyacrylamide: a re-examination.
In this paper the imprinting effects recently reported with protein-imprinted polymers based on chitosan and polyacrylamide are re-assessed. Molecularly imprinted polymers (MIPs) from which the embedded template molecules were removed by washing with a solution of sodium dodecyl sulfate and acetic acid were prepared. In batch template rebinding experiments, the MIPs displayed quite high template binding capacity as reported previously. However, the non-imprinted polymers (NIPs), after washing with the same solution, also showed large binding capacity nearly equal to that of the MIPs. X-ray diffraction and scanning electronic microscope investigations confirmed remarkable property changes of the NIPs after the washing process. These findings indicate that the non-specific adsorption resulting from the template removing process rather than the imprinted sites generated on the MIPs themselves may account for the high template rebinding capacity of the reported protein-imprinted polymers.
Fu
Guo-Qi
GQ
Key Laboratory of Functional Polymers of Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, PR China. gqfu@nankai.edu.cn <gqfu@nankai.edu.cn>
Yu
Hao
H
Zhu
Jing
J
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006454:Hemoglobins / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D012709:Serum Albumin / Q000737:chemistry* / Q000648:ultrastructure; D014961:X-Ray Diffraction; D000099166:Polyacrylamides
D000180:Acrylic Resins; D048271:Chitosan; D003432:Cross-Linking Reagents; D006454:Hemoglobins; D012709:Serum Albumin; D000099166:Polyacrylamides