title: Effects of endogenous ligands on the biological role of human serum albumin in S-nitrosylation.
abstract: Many proteins have been identified as targets for S-nitrosylation, including structural and signaling proteins, a nd ion channels. S-nitrosylation plays an important role in regulating their activity and function. We used human serum albumin (HS A), a major endogenous NO traffic protein, and studied the effect of mediators on S-nitrosylation processes which control NO bioactivity. By using NOC-7, S-nitrosoglutathione, and activated RAW264.7 cells as NO-donors we found that high-affinity binding of endogenous ligands (Cu(2+), bilirubin and fatty acid) can affect these processes. It is likely that the same effects take place in many clinical situations characterized by increased fatty acid concentrations in plasma such as type II diabetes and the metabolic syndrome. Thus, endogenous ligands, changing their plasma concentrations, could be a novel type of mediator of S-nitrosylation not only in the case of HSA but also for other target proteins.
authors.affiliation: Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 O e-honmachi, Kumamoto 862-0973, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ishima
authors.forename: Takaaki
authors.initials: T
authors.lastname: Akaike
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kragh-Hansen
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hiroyama
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Sawa
authors.forename: Toru
authors.initials: T
authors.lastname: Maruyama
authors.forename: Toshiya
authors.initials: T
authors.lastname: Kai
authors.forename: Masaki
authors.initials: M
authors.lastname: Otagiri
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology; D008024:Ligands; D051379:Mice; D008958:Models, Molecular; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009603:Nitroso Compounds / Q000737:chemistry*; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*
chemical_list: C115239:3-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-propanamine; D006834:Hydrazines; D008024:Ligands; D009603:Nitroso Compounds; D012709:Serum Albumin; C001702:mercaptoalbumin