title: Investigation of microencapsulated BSH active lactobacillus in the simulated human GI tract.
abstract: This study investigated the use of microencapsulated bile salt hydrolase (BSH) overproducing Lactobacillus plantarum 80 cells for oral delivery applications using a dynamic computer-controlled model simulating the human gastrointestinal (GI) tract. Bile salt deconjugation rates for microencapsulated BSH overproducing cells were 4.87 +/- 0.28 mumol/g microcapsule/h towards glycoconjugates and 0.79 +/- 0.15 mumol/g microcapsule/h towards tauroconjugates in the simulated intestine, a significant (P< .05) increase over microencapsulated wild-type cells. Microcapsules protected the encased cells in the simulated stomach prior to intestinal release, maintaining cell viability above 109 cfu/mL at pH 2.5 and 3.0 and above 106 cfu/mL at pH 2.0 after 2-hour residence times. In the simulated intestine, encased cell viability was maintained above 1010 cfu/mL after 3, 6, and 12-hour residence times in bile concentrations up to 1.0%. Results show that microencapsulation has potential in the oral delivery of live BSH active bacterial cells. However, in vivo testing is required.
authors.affiliation: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, PQ, Canada H3A 2B4.
authors.forename: Christopher
authors.initials: C
authors.lastname: Martoni
authors.forename: Jasmine
authors.initials: J
authors.lastname: Bhathena
authors.forename: Mitchell Lawrence
authors.initials: ML
authors.lastname: Jones
authors.forename: Aleksandra Malgorzata
authors.initials: AM
authors.lastname: Urbanska
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash