title: Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease.
abstract: This study determined the effects of pcDNA3-beta-nerve growth factor (NGF) gene-modified bone marrow stromal cells (BMSC) on the rat model of Parkinson's disease (PD). The recombinant plasmid pcDNA3-beta-NGF was transfected into BMSC, and NGF expression and its biological activity in vitro were detected. BMSC modified by the NGF gene were then grafted into the corpus striatum of PD rats, and the rotation behavior was evaluated at 1, 2, 4, and 6 weeks post-transplantation. A significant improvement in rotation behavior was observed in PD rats subjected to cell transplantation, especially in PD rats receiving NGF-modified BMSC. The genetically modified BMSC survived and expressed beta-NGF but did not differentiate into tyrosine hydroxylase-positive cells in vivo. The present findings suggested that genetically modified BMSC could be effective for PD treatment, and the mechanisms might involve the neuroprotective effects of beta-NGF.
authors.affiliation: Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China. tinghua_neuron@263.net
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
authors.forename: Zhong-Tang
authors.initials: ZT
authors.lastname: Feng
authors.forename: Peng
authors.initials: P
authors.lastname: Wei
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Shi
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D001522:Behavior, Animal; D016026:Bone Marrow Transplantation / Q000379:methods*; D002454:Cell Differentiation; D003342:Corpus Striatum / Q000166:cytology / Q000502:physiology / Q000601:surgery; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D006085:Graft Survival; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009043:Motor Activity; D020932:Nerve Growth Factor / Q000235:genetics*; D020734:Parkinsonian Disorders / Q000235:genetics* / Q000628:therapy*; D010957:Plasmids; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000235:genetics; D017154:Stromal Cells / Q000637:transplantation*; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D020932:Nerve Growth Factor