title: Reversed-phase isolation of peptides.
abstract: In reversed-phase HPLC, peptides are separated on a hydrophobic stationary phase and eluted with a gradient of increasing organic solvent concentration. Protocols describing the separation of peptides in 5- to 500-pmol quantities via narrow-bore (2-mm-i.d.) or microbore (1-mm-i.d.) columns, as well as for the separation of peptides in quantities <5 pmol are provided in this unit. Capillary HPLC columns require a gradient flow rate of 3 to 5 omponents present in a small sample prior to automated sequencing is possible via the procedures for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry and capillary electrophoresis.
authors.affiliation: Genentech, Inc., South San Francisco, California.
authors.forename: W J
authors.initials: WJ
authors.lastname: Henzel
authors.forename: J T
authors.initials: JT
authors.lastname: Stults
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D010449:Peptide Mapping; D010455:Peptides / Q000302:isolation & purification*; D012997:Solvents; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D012997:Solvents