Pseudomonas fluorescens HYK0210-SK09 offers species-specific biological control of winter algal blooms caused by freshwater diatom Stephanodiscus hantzschii.
OBJECTIVE
The study of an algicidal activity and mechanism of the isolated Pseudomonas fluorescens HYK0210-SK09 (SK09) against a winter bloomed harmful diatom, Stephanodiscus hantzschii.


RESULTS
SK09 was isolated from the Paldang reservoir, Korea and used to biological control of S. hantzschii. The inoculation of SK09 at the final density of 5 x 10(6) cells ml(-1) caused degradation of >90% of S. hantzschii cells within 5 days. The algal cell lysis was achieved by a direct attack of the bacteria to the diatom cells, and the algicidal compound was located in the cytoplasm of the cell. As SK09 did not suppress Microcystis aeruginosa, Anabaena cylindrica, Coelastrum astroideum or Cyclotella meneghiniana, it appeared to attack S. hantzschii in a species-specific manner. Testing in an indoor mesocosms confirmed that SK09 effectively reduced S. hantzschii cells by 88% within 9 days.


CONCLUSIONS
This bacterium is useful in regulating blooms of S. hantzschii. However, it should be studied in the future that their impact in shaping phytoplankton community and their activity in natural environments.


CONCLUSIONS
The bacterium enabled us to develop a new strategy, to understand the interaction for anthropogenic control of harmful algal blooms in nature.
Jung
S W
SW
Department of Life Science, Hanyang University, Seoul, Korea.
Kim
B-H
BH
Katano
T
T
Kong
D-S
DS
Han
M-S
MS
D000898:Antibiosis / Q000502:physiology*; D056890:Eukaryota / Q000254:growth & development*; D005068:Eutrophication / Q000502:physiology*; D005618:Fresh Water; D011551:Pseudomonas fluorescens / Q000502:physiology*; D012621:Seasons; D013045:Species Specificity; D014871:Water Microbiology