Novel promoter sequence required for inductive expression of the Aspergillus nidulans endoglucanase gene eglA.
Expression of the eglA gene, encoding for a major endoglucanse EG A in Aspergillus nidulans, is induced by cellulose and cellobiose, but not by xylose. This suggests that induction is independent of XlnR, a transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus. Mutational analysis of the eglA promoter was performed to identify the novel cis-element responsible for XlnR-independent induction. The region spanning -153 to -138 (CCGTACCTTTTTAGGA), designated CeRE(Cellulose Responsive Element), was found to be the upstream activating element essential to inductive expression of the eglA gene.
Endo
Yoshikazu
Y
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya-shi, Aichi 464-8601, Japan.
Yokoyama
Misako
M
Morimoto
Munenori
M
Shirai
Kengo
K
Chikamatsu
Go
G
Kato
Naoki
N
Tsukagoshi
Norihiro
N
Kato
Masashi
M
Kobayashi
Tetsuo
T
D001233:Aspergillus nidulans / Q000201:enzymology*; D001483:Base Sequence; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D002482:Cellulose / Q000378:metabolism; D017931:DNA Primers; D004271:DNA, Fungal; D004790:Enzyme Induction; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D014990:Xylans / Q000378:metabolism
D017931:DNA Primers; D004271:DNA, Fungal; D014990:Xylans; D002482:Cellulose; D002480:Cellulase