title: The effect of mechanical loading on the metabolism of growth plate chondrocytes.
abstract: It is well known that mechanical loading influences the endochondral bone formation essential for the growth and development of longitudinal bones. The question was, however, asked whether the effect of mechanical loading on the chondrocyte metabolism is dependent on the loading frequency. This study was aimed at evaluating the effect of tensile loadings with various frequencies on the proliferation of growth plate chondrocytes and extracellular matrix synthesis. The chondrocytes obtained from rib growth plate cartilage of 4-week-old male Wistar strain rats were cultured by day 4 and day 11 and used as proliferating and matrix-forming chondrocytes, respectively. Intermittent tensile stresses with different frequencies were applied to each stage chondrocyte. DNA syntheses were examined by measuring the incorporation of [(3)H]thymidine into the cells. Furthermore, the rates of collagen and proteoglycan syntheses were determined by measuring the incorporation of [2,3-(3)H]proline and [(35)S]sulfate into the cells, respectively. At the proliferating stage, intermittent tensions with the frequencies of 30 cycles/min and 150 cycles/min significantly (p < 0.05) upregulated the syntheses of DNA, which indicates the promotion of chondrocyte proliferation. At the matrix-forming stage, collagen, and proteoglycan syntheses also enhanced with increase of the loading frequency. In particular, the intermittent tension with the frequencies of 30 cycles/min and 150 cycles/min increased significantly (p < 0.05 or p < 0.01) both the collagen and proteoglycan syntheses. These results suggest that the proliferation and differentiation of growth plate chondrocytes are regulated by the mechanical loading and that the chondrocyte metabolism enhanced with increase of loading frequency. These may give more insight into the possible mechanism leading to endochondral bone formation.
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Hiroshima, 734-8553, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ueki
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Kotaro
authors.initials: K
authors.lastname: Tanimoto
authors.forename: Clarice
authors.initials: C
authors.lastname: Nishio
authors.forename: Kobun
authors.initials: K
authors.lastname: Honda
authors.forename: Yu-Yu
authors.initials: YY
authors.lastname: Lin
authors.forename: Yuki
authors.initials: Y
authors.lastname: Tanne
authors.forename: Satoru
authors.initials: S
authors.lastname: Ohkuma
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamiya
authors.forename: Eiji
authors.initials: E
authors.lastname: Tanaka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanne
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D003094:Collagen / Q000378:metabolism*; D005109:Extracellular Matrix / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006132:Growth Plate / Q000166:cytology / Q000502:physiology*; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013718:Tensile Strength / Q000502:physiology; D016474:Weight-Bearing / Q000502:physiology
chemical_list: D016326:Extracellular Matrix Proteins; D011509:Proteoglycans; D003094:Collagen