title: Physiological changes and molluscicidal effects of crude latex and Milin on Biomphalaria glabrata.
abstract: Euphorbian latex is commonly used as molluscicides and the Euphorbia milii latex was reported as most powerful molluscicidal agents. The physiological and lethal effects of the latex components of Euphorbia milii, on the intermediate host Biomphalaria spp., of the human liver parasite Schistosoma mansoni were described in this study. The standard methodologies for testing plant derived molluscicides formulated by World Health Organisation (WHO) were followed with some modifications. The young specimen of fresh water snails showed altered physiological and physical response towards latex components. The working concentration of non-proteinaceous fraction (up to 0.1%) of the latex reduced the active physiological behaviour but was non-lethal to young specimen of snails. However, proteinaceous fractions (0.1mg/l) of the latex were found lethal to snail population, and lethality was enhanced with small amount of the non-proteinaceous fraction (0.01%) of the latex. Milin, a serine protease(up to 0.1mg/l), isolated from the latex of Euphorbia milii significantly reduced the growth and feeding activity but was not lethal to young specimen of snails. With an addition of 0.01% of non-proteinaceous fractions to Milin, lethality result was similar to that of crude latex. Milin is likely to be responsible for alteration of normal physiological functions and lethality of snails, thus it may be used as a molluscicide to control transmission of the endemic disease schistosomiasis.
authors.affiliation: Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India.
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Yadav
authors.forename: M V
authors.initials: MV
authors.lastname: Jagannadham
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000187:drug effects / Q000469:parasitology; D028481:Euphorbia / Q000737:chemistry* / Q000201:enzymology; D007840:Latex / Q000737:chemistry / Q000494:pharmacology*; D008975:Molluscacides / Q000494:pharmacology*; D010571:Pest Control / Q000379:methods*; D012550:Schistosoma mansoni / Q000187:drug effects / Q000502:physiology; D012555:Schistosomiasis mansoni / Q000517:prevention & control; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D007840:Latex; D008975:Molluscacides; D012697:Serine Endopeptidases