Antimutagenic effects of piperine on cyclophosphamide-induced chromosome aberrations in rat bone marrow cells.
Piperine is a major pungent substance and active component of black pepper (Piper nigrum Linn.) and long pepper (Piper longum Linn.). Both plants are used worldwide as household spices and condiments. They are also used as important ingredients in folklore medicine in many Asian countries. Therefore, it is of interest to study antimutagenic effects of piperine. In this study, its influence on chromosomes was investigated in rat bone marrow cells. Male Wistar rats were orally administered piperine at the doses of 100, 400 and 800 mg/kg body weight for 24 hours then challenged with cyclophosphamide at a dose of 50 mg/kg body weight by intraperitoneal injection. Twenty-four hours thereafter, all animals were sacrificed and bone marrow samples were collected for chromosomal analysis. The results demonstrated that piperine at a dose of 100 mg/kg body weight gave a statistically significant reduction in cyclophosphamide-induced chromosomal aberrations. In conclusion, piperine may have antimutagenic potential. The underlying molecular mechanisms now require attention.
Wongpa
Sareeya
S
Toxicology Graduate Programme, Faculty of Science, Mahidol University, Bangkok, Thailand. noo_toxmu@hotmail.com
Himakoun
Lakana
L
Soontornchai
Sarisak
S
Temcharoen
Punya
P
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity; D052117:Benzodioxoles / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects*; D003520:Cyclophosphamide / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D008297:Male; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index; D029222:Piper nigrum / Q000737:chemistry; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D000470:Alkaloids; D016587:Antimutagenic Agents; D018906:Antineoplastic Agents, Alkylating; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D003520:Cyclophosphamide; C008922:piperine