Patterned immobilization of antibodies in mechanically induced cracks.
A new approach of chemically immobilizing antibody within a pattern based on thin-film cracking is presented. An adjustable pattern width is achieved with resolutions varied from nano- to microscale by using loading stress on thin-film coated elastomer substrate in both one and two dimensions. By introduction of solution or chemical vapor deposition approaches, antibodies were covalently immobilized in the channels. To demonstrate the bioactivity, specificity, and response rate of antibody patterned structure, scanning electron microscopy was used to enumerating bacteria. The chemically coupled antibody is found to retain its specificity when incubated with different bacteria solutions. Trichloro(1H,1H,2H,2H-perfluoroctyl)silane coating on nonsensing regions exhibits a distinguished bacteria-resistant function that is beneficial for providing a low intrinsic background signal in detection. This technique shows a great potential for applications in the fields of sensing and tissue engineering.
Cao
Ting
T
Department of Chemical Engineering and Materials Science and Department of Electrical and Computer Engineering, Wayne State University, 5050 Anthony Wayne Drive, Detroit, Michigan 48202, USA.
Wang
Anfeng
A
Liang
Xuemei
X
Tang
Haiying
H
Auner
Gregory W
GW
Salley
Steven O
SO
Ng
K Y Simon
KY
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001427:Bacterial Toxins / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D004926:Escherichia coli / Q000166:cytology / Q000276:immunology; D007700:Kinetics; D019563:Mechanics; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D012826:Silicone Elastomers / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001427:Bacterial Toxins; C031429:K99 antigen; D012826:Silicone Elastomers