[Surface markers and functions of human dendritic cells exposed to mobile phone 1800 MHz electromagnetic fields].
To investigate the effects of mobile phone 1800 MHz electromagnetic fields (EMF) on the surface markers and the functions of human dendritic cells (DC).



Human DCs were exposed to intermittent 5 min on/10 min off EMF with specific absorption rates (SAR) 4 W/kg for 0 h, 1 h, 12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CD80, CD86, CD40 and CD11c. CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction (allo-MLR) of DC, and enzyme linked immunosorbent assay (ELISA) to identify the levels of IL-12p70 and TNF-alpha secreted by DC.



Compared with the sham radiation group, after exposure to the electromagnetic fields for 1 h, 12 h, or 24 h, HLA-DR, CD80,CD86 and CD40 were all declined except CD11c. The ability of DC allo-MLR in each exposure group was decreased significantly (P<0.05), especially in the 24 h exposure group. However, the secreted levels of IL-12p70 and TNF-alpha of DC in each exposure group remained no changed.



The study showed that EMF exposure could down-regulate the surface molecules and stimulation ability of human DC.
Zhou
Zhi-dong
ZD
Institute of Immunology, College of Medicine, Zhejiang University, Hangzhou 310058, China.
Zeng
Qun-li
QL
Zheng
Yun
Y
Zhang
Jian-bin
JB
Chen
Hai-yang
HY
Lu
De-qiang
DQ
Shao
Chuan-sen
CS
Xia
Da-jing
DJ
D018122:B7-1 Antigen; D051940:B7-2 Antigen / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D039521:CD11c Antigen / Q000276:immunology; D040421:Cell Phone; D002478:Cells, Cultured; D003712:Dendrites / Q000473:pathology; D003713:Dendritic Cells / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*; D004574:Electromagnetic Fields; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D018664:Interleukin-12 / Q000276:immunology
D018122:B7-1 Antigen; D051940:B7-2 Antigen; D015415:Biomarkers; D039521:CD11c Antigen; D006684:HLA-DR Antigens; D018664:Interleukin-12