title: Molecular identification of Trypanosoma cruzi I tropism for central nervous system in Chagas reactivation due to AIDS.
abstract: Trypanosoma cruzi lineages, microsatellite allelic polymorphism, and mithocondrial gene haplotypes were directly typified from peripheral blood and cerebrospinal fluid specimens of a Bolivian patient with Chagas disease with accompanying AIDS and central nervous system severe involvement. Of note, the patient's blood was infected by a mixture of T. cruzi I and T. cruzi IId/e polyclonal populations while the cerebrospinal fluid showed only a monoclonal T. cruzi I population. Our findings do not corroborate the original assumption of innocuity for T. cruzi I in the southern cone of the Americas and highlight lineage I tropism for central nervous system causing lethal Chagas reactivation.
authors.affiliation: Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Burgos
authors.forename: Sandra
authors.initials: S
authors.lastname: Begher
authors.forename: Helder M Valadares
authors.initials: HM
authors.lastname: Silva
authors.forename: Margarita
authors.initials: M
authors.lastname: Bisio
authors.forename: Tomas
authors.initials: T
authors.lastname: Duffy
authors.forename: Mariano J
authors.initials: MJ
authors.lastname: Levin
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Macedo
authors.forename: Alejandro G
authors.initials: AG
authors.lastname: Schijman
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000469:parasitology*; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D000818:Animals; D001838:Bolivia; D002490:Central Nervous System / Q000469:parasitology; D020808:Central Nervous System Protozoal Infections / Q000175:diagnosis / Q000209:etiology / Q000469:parasitology*; D014355:Chagas Disease / Q000209:etiology* / Q000469:parasitology; D016054:DNA, Protozoan / Q000097:blood / Q000134:cerebrospinal fluid; D003576:Electron Transport Complex IV / Q000235:genetics; D017809:Fatal Outcome; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012008:Recurrence; D018523:Tropism / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D016054:DNA, Protozoan; D003576:Electron Transport Complex IV