Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient.
In order to investigate the potential of a Sanguisorba officinalis root extract as an active ingredient for wrinkle-care cosmetics, we measured its free radical scavenging activity, elastase inhibitory activity, expression of MMP-1 (matrix metalloprotease-1) in vitro, and type I collagen synthesis in normal human fibroblast cells. To isolate the main components from the S. officinalis root extract, we purified the extract by solvent fractionation, column chromatography, and recrystallization. The active component was identified as ziyuglycoside I by a spectroscopic analysis. Ziyuglycoside I increased the expression of type I collagen in a dose-dependent manner (by up to 71.3% at 50 muM). A clinical study of a formulation containing ziyuglycoside I, which involved visual evaluation and image analysis, showed a significantly different effect (p<0.05) of the test formulation from that of the placebo. This result suggests that ziyuglycoside I isolated from S. officinalis root extract could be used as an active ingredient for cosmetics.
Kim
Young Heui
YH
R&D Center, Bioland Ltd., Songjeong, Byongchon, Cheonan, Chungnam 330-863, Republic of Korea.
Chung
Chan Bok
CB
Kim
Jin Guk
JG
Ko
Kang Il
KI
Park
Sun Hee
SH
Kim
Jong-Heon
JH
Eom
Sang Yong
SY
Kim
Young Sil
YS
Hwang
Yong-Il
YI
Kim
Ki Ho
KH
D000328:Adult; D001483:Base Sequence; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D003358:Cosmetics; D003460:Crystallization; D017931:DNA Primers; D004311:Double-Blind Method; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D008875:Middle Aged; D010919:Placebos; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D015595:Skin Aging / Q000187:drug effects*; D013056:Spectrophotometry, Ultraviolet
D024042:Collagen Type I; D003358:Cosmetics; D017931:DNA Primers; D016166:Free Radical Scavengers; D010919:Placebos; D010936:Plant Extracts; D012503:Saponins; C484848:ziyuglycoside I; D020781:Matrix Metalloproteinase 1