title: Antimicrobial activity of Rheedia brasiliensis and 7-epiclusianone against Streptococcus mutans.
abstract: This in vitro study evaluated the antimicrobial activity of extracts obtained from Rheedia brasiliensis fruit (bacupari) and its bioactive compound against Streptococcus mutans. Hexane, ethyl-acetate and ethanolic extracts obtained (concentrations ranging from 6.25 to 800 microg/ml) were tested against S. mutans UA159 through MIC/MBC assays. S. mutans 5-days-old biofilms were treated with the active extracts (100 x MIC) for 0, 1, 2, 3 and 4h (time-kill) and plated for colony counting (CFU/ml). Active extracts were submitted to exploratory chemical analyses so as to isolate and identify the bioactive compound using spectroscopic methods. The bioactive compound (concentrations ranging from 0.625 to 80 microg/ml) was then tested through MIC/MBC assays. Peel and seed hexane extracts showed antimicrobial activity against planktonic cells at low concentrations and were thus selected for the time kill test. These hexane extracts reduced S. mutans biofilm viability after 4h, certifying of the bioactive compound presence. The bioactive compound identified was the polyprenylated benzophenone 7-epiclusianone, which showed a good antimicrobial activity at low concentrations (MIC: 1.25-2.5 microg/ml; MBC: 10-20 microg/ml). The results indicated that 7-epiclusianone may be used as a new agent to control S. mutans biofilms; however, more studies are needed to further elucidate the mechanisms of action and the anticariogenic potential of such compound found in R. brasiliensis.
authors.affiliation: Department of Physiological Sciences, Faculty of Dentistry of Piracicaba, State University of Campinas, SP, Brazil.
authors.forename: L S B
authors.initials: LS
authors.lastname: Almeida
authors.forename: R M
authors.initials: RM
authors.lastname: Murata
authors.forename: R
authors.initials: R
authors.lastname: Yatsuda
authors.forename: M H
authors.initials: MH
authors.lastname: Dos Santos
authors.forename: T J
authors.initials: TJ
authors.lastname: Nagem
authors.forename: S M
authors.initials: SM
authors.lastname: Alencar
authors.forename: H
authors.initials: H
authors.lastname: Koo
authors.forename: P L
authors.initials: PL
authors.lastname: Rosalen
mesh_terms: D001577:Benzophenones / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D018441:Biofilms; D028084:Clusiaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: C118110:7-epiclusianone; D001577:Benzophenones; D016227:Benzoquinones; D010936:Plant Extracts