Bactericidal and virucidal activity of the alkalophilic P395D/L241V/T343A mutant of vanadium chloroperoxidase.
OBJECTIVE
Vanadium chloroperoxidase and its directed evolution mutant P395D/L241V/T343A were investigated for their antibacterial and antiviral potential at slightly alkaline pH and at a H(2)O(2) concentration that is low compared to current nonenzymatic formulations.


RESULTS
Two bacteria (the Gram-negative Pseudomonas aeruginosa and the Gram-positive Staphylococcus aureus) and two viruses (the enveloped Herpes Simplex Virus and the nonenveloped Coxsackievirus B4) were incubated with the P395D/L241V/T343A mutant, 10 mmol l(-1) H(2)O(2) and 100 mmol l(-1) Br(-) at pH 8. Strong microbial reduction was observed and bactericidal and virucidal activities of the mutant were three to six orders of magnitude higher than for the wild-type enzyme.


CONCLUSIONS
The P395D/L241V/T343A mutant of vanadium chloroperoxidase has a broad antimicrobial activity at alkaline conditions.


CONCLUSIONS
For many disinfection formulations, antimicrobial activity at slightly alkaline pH values is required. To date, only the wild-type vanadium chloroperoxidase has been studied for its antibacterial activity, and only at acidic to neutral pH values. Its antiviral activity (e.g. useful for the cleaning of medical equipment) was not studied before. The observed activity for the alkalophilic P395D/L241V/T343A mutant is an important step forward in the application of this robust enzyme as a component in disinfection formulations.
Renirie
R
R
LCOM, Equipe Oxydation et Formulation, UMR CNRS 8009, ENSCL, Villeneuve d'Ascq, France. rokus100@gmail.com
Dewilde
A
A
Pierlot
C
C
Wever
R
R
Hober
D
D
Aubry
J-M
JM
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology; D002711:Chloride Peroxidase / Q000235:genetics / Q000494:pharmacology*; D019020:Directed Molecular Evolution; D004203:Disinfection / Q000379:methods*; D004770:Enterovirus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D016296:Mutagenesis; D011549:Pseudomonas / Q000187:drug effects; D018139:Simplexvirus / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D038301:Virus Inactivation
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000998:Antiviral Agents; C418625:vanadium chloroperoxidase; D002711:Chloride Peroxidase