title: High Mycoplasma genitalium organism burden is associated with shedding of HIV-1 DNA from the cervix.
abstract: We assessed the relationship between infection with Mycoplasma genitalium, an emerging sexually transmitted pathogen, and cervical shedding of human immunodeficiency virus (HIV)-1 DNA among 303 HIV-1-positive Kenyan women. HIV-1 shedding was detected by qualitative polymerase chain reaction (PCR) in 154 women (51%); M. genitalium was detected by qualitative PCR in 52 (17%), and organism burden was determined by quantitative PCR. Women with high M. genitalium organism burdens (more than the median of 3195 genomes/mL) were 3-fold more likely to shed HIV-1 DNA than were M. genitalium-negative women (adjusted OR, 2.9 [95% confidence interval, 1.1-7.6]), yet this did not appear to be mediated by traditional measures of cervical inflammation (elevated polymorphonuclear leukocyte count).
authors.affiliation: Department of Epidemiology, University of Washington, Seattle, WA 98104-2499, USA. lmanhart@u-washington.edu
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Manhart
authors.forename: Sara B
authors.initials: SB
authors.lastname: Mostad
authors.forename: Jared M
authors.initials: JM
authors.lastname: Baeten
authors.forename: Sabina G
authors.initials: SG
authors.lastname: Astete
authors.forename: Kishorchandra
authors.initials: K
authors.lastname: Mandaliya
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Totten
mesh_terms: D000293:Adolescent; D000328:Adult; D002584:Cervix Uteri / Q000821:virology*; D004279:DNA, Viral; D005260:Female; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1; D006801:Humans; D007630:Kenya; D008875:Middle Aged; D009175:Mycoplasma Infections / Q000150:complications*; D045704:Mycoplasma genitalium / Q000472:pathogenicity*; D016017:Odds Ratio; D017201:Virus Shedding
chemical_list: D004279:DNA, Viral