Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites.
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtained demonstrate high heterozygosity (average of 0.69) in a Lower Amazon population. The multiplex system described can thus be considered a fast, efficient and inexpensive method for the investigation of genetic variability in Arapaima populations.
Hamoy
I G
IG
Laboratório de Genética Humana e Médica, Centro de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil. ighamoy@yahoo.com.br
Santos
E J M
EJ
Santos
S E B
SE
D000483:Alleles; D000818:Animals; D001938:Brazil; D017931:DNA Primers / Q000737:chemistry; D005399:Fishes / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods*; D011110:Polymorphism, Genetic; D015203:Reproducibility of Results; D013997:Time Factors
D017931:DNA Primers; D005819:Genetic Markers