title: The influence of 2,4,6-tribromophenyl isocyanate on the cellular and nuclear uptake of the SV 40 T antigen nuclear localization sequence.
abstract: The objective of the present study was to evaluate the influence of 2,4,6-tribromophenyl isocyanate (TBPI) on the cellular and nuclear uptake of the fluorescein isothiocyanate (FITC) labeled SV 40 T antigen nuclear localization sequence in human LN18 and U373 glioma cells. Therefore, the FITC-labeled nuclear localization sequence (NLS) of the SV 40 T antigen was coupled to 2,4,6-TBPI. This TBPI-NLS conjugate was taken up by the cell nuclei of more than 90% of human malignant glioma cells. The nuclearly stained cells showed clear signs of cell death. However only up to 10% of the cells were stained after incubation with the TBPI-lacking NLS of the SV 40 T antigen together with free, unbound TBPI. These cells stayed alive. TBPI, when bound to small peptides, may be an important component for future drugs against gliomas.
authors.affiliation: Department of Neuroradiology, University of Tübingen, Germany. stefan.heckl@med.uni-tuebingen.de <stefan.heckl@med.uni-tuebingen.de>
authors.forename: Stefan
authors.initials: S
authors.lastname: Heckl
authors.forename: Alexander
authors.initials: A
authors.lastname: Sturzu
authors.forename: Alireza
authors.initials: A
authors.lastname: Gharabaghi
authors.forename: Hartmut
authors.initials: H
authors.lastname: Echner
authors.forename: Thomas
authors.initials: T
authors.lastname: Nagele
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D001693:Biological Transport, Active; D001932:Brain Neoplasms / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005910:Glioma / Q000378:metabolism; D006801:Humans; D017953:Isocyanates / Q000494:pharmacology*; D018613:Microscopy, Confocal; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C529030:2,4,6-tribromophenyl isocyanate; D000952:Antigens, Polyomavirus Transforming; D005456:Fluorescent Dyes; D017953:Isocyanates; D016650:Fluorescein-5-isothiocyanate