title: Dietary lipids alter the effect of steroids on the transport of fructose following intestinal resection in rats.
abstract: BACKGROUND
  Glucocorticosteroids alter intestinal morphology and transport. We tested the hypothesis that the desired intestinal adaptive response following intestinal resection may be enhanced further by the locally active steroid budesonide, and by feeding a saturated as compared with a polyunsaturated fatty acid diet.
  
  
  METHODS
  An in-vitro uptake method was used to assess intestinal fructose uptake by rats of semisynthetic diets enriched in saturated or polyunsaturated fatty acids, and injected with budesonide or control solution.
  
  
  RESULTS
  Budesonide increased ileal fructose uptake in chow and PUFA-fed animals, but reduced jejunal fructose uptake in rats fed SFA. GLUT5 and GLUT2 protein and mRNA did not correlate with changes in fructose uptake. Steroids reduced jejunal proglucagon expression in animals fed chow. Animals fed SFA and given budesonide had a reduction in jejunal ODC mRNA compared with those fed PUFA or chow.
  
  
  CONCLUSIONS
  (1) budesonide increases ileal fructose uptake following intestinal resection, and this beneficial effect is prevented by feeding SFA rather than PUFA; (2) fructose uptake does not correlate with GLUT5 and GLUT2 protein and mRNA; (3) ODC and proglucagon may be involved in this adaptive response.
authors.affiliation: Nutrition and Metabolism Research Group, Division of Gastroenterology, Department of Medicine, University of Alberta, Edmonton, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Thiesen
authors.forename: K A
authors.initials: KA
authors.lastname: Tappenden
authors.forename: M I
authors.initials: MI
authors.lastname: McBurney
authors.forename: M T
authors.initials: MT
authors.lastname: Clandinin
authors.forename: M
authors.initials: M
authors.lastname: Keelan
authors.forename: B K A
authors.initials: BK
authors.lastname: Thomson
authors.forename: L A
authors.initials: LA
authors.lastname: Drozdowski
authors.forename: G
authors.initials: G
authors.lastname: Wild
authors.forename: A B R
authors.initials: AB
authors.lastname: Thomson
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D019819:Budesonide / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005632:Fructose / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology*; D051273:Glucose Transporter Type 2 / Q000235:genetics / Q000378:metabolism; D051296:Glucose Transporter Type 5 / Q000235:genetics / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects*; D007421:Intestine, Small / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011184:Postoperative Period; D052336:Proglucagon / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005938:Glucocorticoids; D051273:Glucose Transporter Type 2; D051296:Glucose Transporter Type 5; D012333:RNA, Messenger; C495683:Slc2a2 protein, rat; C495727:Slc2a5 protein, rat; D005632:Fructose; D019819:Budesonide; D052336:Proglucagon