title: Bulky DNA adducts, 4-aminobiphenyl-haemoglobin adducts and diet in the European Prospective Investigation into Cancer and Nutrition (EPIC) prospective study.
abstract: In contrast to some extensively examined food mutagens, for example, aflatoxins, N-nitrosamines and heterocyclic amines, some other food contaminants, in particular polycyclic aromatic hydrocarbons (PAH) and other aromatic compounds, have received less attention. Therefore, exploring the relationships between dietary habits and the levels of biomarkers related to exposure to aromatic compounds is highly relevant. We have investigated in the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort the association between dietary items (food groups and nutrients) and aromatic DNA adducts and 4-aminobiphenyl-Hb adducts. Both types of adducts are biomarkers of carcinogen exposure and possibly of cancer risk, and were measured, respectively, in leucocytes and erythrocytes of 1086 (DNA adducts) and 190 (Hb adducts) non-smokers. An inverse, statistically significant, association has been found between DNA adduct levels and dietary fibre intake (P = 0.02), vitamin E (P = 0.04) and alcohol (P = 0.03) but not with other nutrients or food groups. Also, an inverse association between fibre and fruit intake, and BMI and 4-aminobiphenyl-Hb adducts (P = 0.03, 0.04, and 0.03 respectively) was observed. After multivariate regression analysis these inverse correlations remained statistically significant, except for the correlation adducts v. fruit intake. The present study suggests that fibre intake in the usual range can modify the level of DNA or Hb aromatic adducts, but such role seems to be quantitatively modest. Fibres could reduce the formation of DNA adducts in different manners, by diluting potential food mutagens and carcinogens in the gastrointestinal tract, by speeding their transit through the colon and by binding carcinogenic substances.
authors.affiliation: Cancer Risk Factor Branch, Analytical and Biomolecular Cytology Unit, CSPO-Scientific Institute of Tuscany, Florence, Italy.
authors.forename: Marco
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authors.lastname: Peluso
authors.forename: Luisa
authors.initials: L
authors.lastname: Airoldi
authors.forename: Armelle
authors.initials: A
authors.lastname: Munnia
authors.forename: Alessandro
authors.initials: A
authors.lastname: Colombi
authors.forename: Fabrizio
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authors.lastname: Veglia
authors.forename: Herman
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authors.lastname: Autrup
authors.forename: Alison
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authors.lastname: Dunning
authors.forename: Seymour
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authors.forename: Christian
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authors.initials: O
authors.lastname: Raaschou-Nielsen
authors.forename: Francoise
authors.initials: F
authors.lastname: Clavel-Chapelon
authors.forename: Jacob
authors.initials: J
authors.lastname: Linseisen
authors.forename: Heiner
authors.initials: H
authors.lastname: Boeing
authors.forename: Antonia
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authors.lastname: Trichopoulou
authors.forename: Domenico
authors.initials: D
authors.lastname: Palli
authors.forename: Vittorio
authors.initials: V
authors.lastname: Krogh
authors.forename: Rosario
authors.initials: R
authors.lastname: Tumino
authors.forename: Salvatore
authors.initials: S
authors.lastname: Panico
authors.forename: Bas H
authors.initials: BH
authors.lastname: Bueno-De-Mesquita
authors.forename: Petra H
authors.initials: PH
authors.lastname: Peeters
authors.forename: Merethe
authors.initials: M
authors.lastname: Kumle
authors.forename: Antonio
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authors.lastname: Agudo
authors.forename: Carmen
authors.initials: C
authors.lastname: Martinez
authors.forename: Miren
authors.initials: M
authors.lastname: Dorronsoro
authors.forename: Aurelio
authors.initials: A
authors.lastname: Barricarte
authors.forename: Marìa Jose
authors.initials: MJ
authors.lastname: Tormo
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Quiros
authors.forename: Goran
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authors.lastname: Berglund
authors.forename: Bengt
authors.initials: B
authors.lastname: Jarvholm
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Day
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Key
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Saracci
authors.forename: Rudolf
authors.initials: R
authors.lastname: Kaaks
authors.forename: Elio
authors.initials: E
authors.lastname: Riboli
authors.forename: Shelia
authors.initials: S
authors.lastname: Bingham
authors.forename: Paolo
authors.initials: P
authors.lastname: Vineis
mesh_terms: D000368:Aged; D000393:Air Pollutants / Q000633:toxicity; D000428:Alcohol Drinking; D015415:Biomarkers / Q000032:analysis; D015992:Body Mass Index; D002273:Carcinogens / Q000032:analysis* / Q000378:metabolism; D003110:Colonic Neoplasms / Q000517:prevention & control; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D004043:Dietary Fiber / Q000008:administration & dosage*; D004912:Erythrocytes / Q000737:chemistry*; D005060:Europe; D007887:Fabaceae; D005260:Female; D005638:Fruit; D006454:Hemoglobins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009747:Nutritional Physiological Phenomena; D010126:Ozone / Q000633:toxicity; D011446:Prospective Studies; D014675:Vegetables
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D002273:Carcinogens; D018736:DNA Adducts; D004043:Dietary Fiber; D006454:Hemoglobins; C062091:hemoglobin, 4-aminobiphenyl-; D010126:Ozone