title: cDNA libraries.
abstract: This unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double-stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be necessary to include the clone of interest, and choices of linkers or adaptors for insertion into the vector.
authors.affiliation: Massachusetts General Hospital And Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Moore
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger