title: Determination of phenolic compounds and ascorbic acid in different fractions of tomato by capillary electrophoresis with electrochemical detection.
abstract: Tomato (Lycopersicon esculentum Mill.), one of the most important crops worldwide, contains different classes of substances with antioxidant properties such as carotenoids, vitamin C, and phenolics. A method based on capillary electrophoresis with electrochemical detection has been developed to analyze ascorbic acid and phenolics in the peel, pulp, and seeds of tomatoes. Operating in a wall-jet configuration, a 300 microm diameter carbon disk electrode was used as the working electrode, which exhibits a good response at +0.90 V (vs saturated calomel electrode) for the analytes. Under optimum conditions, the analytes were baseline separated within 20 min in a 50 mmol/L borate buffer (pH 8.7). Notably, excellent linearity was obtained over 3 orders of magnitude with detection limits (S/N=3) ranging from 1x10(-8) to 2x10(-7) g/mL for all analytes. This proposed method has been successfully applied to monitor the content of ascorbic acid and phenolics in real samples, and the assay results were satisfactory.
authors.affiliation: Department of Chemistry, Quanzhou Normal University, Quanzhou, China. youyuanpeng@hotmail.com
authors.forename: Youyuan
authors.initials: Y
authors.lastname: Peng
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiannong
authors.initials: J
authors.lastname: Ye
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002021:Buffers; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D018551:Solanum lycopersicum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D002021:Buffers; D010636:Phenols; D001205:Ascorbic Acid