Autoregulation of XTcf-4 depends on a Lef/Tcf site on the XTcf-4 promoter.
The restricted expression of XTcf-4 in the anterior midbrain is regulated via an active wnt/beta-catenin pathway (Kunz et al.,2004, Dev Biol 273:390-401). The molecular mechanism of this autoregulatory loop, however, remained elusive. Here we show that the activity of a 1,775 bp promoter fragment containing a consensus Lef/Tcf binding site at position -1,437 to -1,428 is upregulated by activating transcription factors of the Lef/Tcf family. Furthermore, chromatin immunoprecipitation revealed that endogenous beta-catenin is bound to the Lef/Tcf site on the promoter. Thus, regulation of XTcf-4 by canonical wnt-signaling is directly controlled by binding to and activating a consensus Lef/Tcf binding site within its own promoter.
Koenig
Sabrina F
SF
Lattanzio
Roberto
R
Mansperger
Katrin
K
Rupp
Ralph A W
RA
Wedlich
Doris
D
Gradl
Dietmar
D
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D051820:TCF Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D058118:Transcription Factor 7-Like 2 Protein; D014162:Transfection; D051153:Wnt Proteins / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis
D051820:TCF Transcription Factors; C544894:TCF7L2 protein, human; D058118:Transcription Factor 7-Like 2 Protein; D051153:Wnt Proteins; D029867:Xenopus Proteins; C544896:tcf7l2 protein, Xenopus