Production of monoclonal antibody supernatant and ascites fluid.
Procedures detailing the production of monoclonal antibody supernatants, including the production of larger amounts (liters) are presented in this unit. An alternate protocol for large-scale production of hybridomas or cells (e.g., for isolation of cellular proteins) involves a similar procedure. A method for producing and obtaining ascites fluid containing the monoclonal antibody is also presented.
Yokoyama
W M
WM
University of California School of Medicine, San Francisco, California, USA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000378:metabolism*; D001201:Ascites / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D006825:Hybridomas; D007158:Immunologic Techniques
D000911:Antibodies, Monoclonal