title: Cryosectioning.
abstract: This unit describes sample preparation and sectioning methods for frozen tissue. Sections of this type are used in a variety of light microscopic procedures including in situ hybridization, immunohistochemistry, and enzyme histochemistry.
authors.affiliation: Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
authors.forename: S
authors.initials: S
authors.lastname: Watkins
mesh_terms: D000818:Animals; D016616:Cryoultramicrotomy / Q000379:methods*; D017403:In Situ Hybridization; D013395:Sucrose; D016707:Tissue Fixation
chemical_list: D013395:Sucrose