P. gingivalis accelerates gingival epithelial cell progression through the cell cycle.
P. gingivalis, an opportunistic pathogen in periodontal disease, can reside within the epithelial cells that line the gingival crevice. A proteomic analysis revealed that infection of gingival epithelial cells with P. gingivalis induces broadly based changes in the level and phosphorylation status of proteins that exert multi-level control on the eukaryotic cell cycle. Pathways that were impacted by P. gingivalis included those involving cyclins, p53 and PI3K. The predicted infection-dependent phenotype was confirmed by cytofluorimetry that showed an enhanced proliferation rate of gingival epithelial cells infected with P. gingivalis associated with accelerated progression through the S-phase. Elevated cell proliferation was dependent on the presence of the long fimbriae of P. gingivalis. The ability of P. gingivalis, a common inhabitant of the subgingival crevice, to accelerate cell cycling could have biological consequences for barrier and signaling functions, and for physiological status, of the gingival epithelium.
Kuboniwa
Masae
M
Department of Preventive Dentistry, Osaka University Graduate School of Dentistry, Osaka, Japan.
Hasegawa
Yoshiaki
Y
Mao
Song
S
Shizukuishi
Satoshi
S
Amano
Atsuo
A
Lamont
Richard J
RJ
Yilmaz
Ozlem
O
D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000737:chemistry / Q000382:microbiology*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology; D006801:Humans; D016966:Porphyromonas gingivalis / Q000502:physiology*; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology; D020543:Proteome / Q000032:analysis
D011506:Proteins; D020543:Proteome