Electrophoresis PDMS/glass chips with continuous on-chip derivatization and analysis of amino acids using naphthalene-2,3-dicarboxaldehyde as fluorogenic agent.
In this work, we developed a PDMS electrophoresis device able to carry out on-chip derivatization and quantification of amino acids (AAs) using naphthalene-2,3-dicarboxaldehyde (NDA) as a fluorogenic agent. A chemical modification of the PDMS surface was found compulsory to achieve the derivatization of AAs with NDA and a limit of detection (LOD) of 40nM was reached for glycine. Finally, we suggested the applicability of this microdevice for the analysis of real biological samples such as a rat hippocampus microdialysate.
Yassine
O
O
Institut des Nanotechnologies de Lyon, INL, CNRS UMR5270, Université de Lyon, F-69003 Lyon, France.
Morin
P
P
Dispagne
O
O
Renaud
L
L
Denoroy
L
L
Kleimann
P
P
Faure
K
K
Rocca
J-L
JL
Ouaini
N
N
Ferrigno
R
R
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D005898:Glass; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism; D009281:Naphthalenes / Q000032:analysis* / Q000737:chemistry; D051381:Rats
D000596:Amino Acids; D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde