[Complex of recombinant heat shock protein with lipopolysaccharide induces rapid protection of mice against Salmonella typhimurium and avirulent for humans avian influenza virus H5N2].
Protective effect of immunization with heat shock protein (HSP) against bacterial and viral infections in mice was studied. Recombinant HSP 70 kDa of Mycobacterium tuberculosis contaminated with lypopolysaccharide (0.185 mcg/ml) was used for experiments. One intraperitoneal injection of 100 or 400 mcg of HSP induced rapid protection against intraperitoneal challen e with 125 LD50 of Salmonella typhimurium (on 3rd-6th day) and against intranasal challenge with 10 LD50 of avirulent for humans avian influenza virus H5N2 (A/ mallard/Pennsylvania/10218/84) (on 5th-8th day). Three daily injections with 10 mcg of HSP induced rapid, significant and long-term protection against S. typhimurium. Immunization with HSP protected 100% of mice during 3 days after the challenge, 50% of immunized animals survived during 21 days (duration of the study). All nonimmunized mice died on 6th day.
Semenova
I B
IB
Vorob'ev
D S
DS
Sveshnikov
P G
PG
Kurbatova
E A
EA
D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000737:chemistry; D006360:Heat-Shock Proteins / Q000008:administration & dosage* / Q000737:chemistry; D007115:Immunization Schedule; D053125:Influenza A Virus, H5N2 Subtype; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D051379:Mice; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D012486:Salmonella typhimurium; D014611:Vaccination; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000737:chemistry; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000737:chemistry
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D017778:Vaccines, Combined; D014614:Vaccines, Synthetic