Micronuclei assessment in the urothelial cells of women using hair dyes and its modulation by genetic polymorphisms.
Increases in the frequency of micronuclei (MN) in exposed cells can be used as a measure of genotoxicity. Hair dyes contain chemicals that are eliminated by urine and could be genotoxic to urothelial cells. To address this question, we evaluated whether hair dye use is associated with an increase in the frequency of MN in urothelial cells, and whether this association is modified by NAT1 (N-acetyltransferase 1), NAT2 (N-acetyltransferase 2) and GSTM1 (glutathione-S-transferase M1) genotypes. We included 92 women participating as controls in a bladder cancer case-control study in Spain. Of those, 72 had adequate number of cells to be included in the MN analysis. There were no significant differences in the mean MN frequency in women using hair dyes in the last month (9.88 MN/1000 cells), in comparison with the MN in unexposed women (9.50 MN/1000 cells). No statistically significant differences in MN frequency were observed by type of hair dye or color of the hair dye. Comparison of subjects in the highest quartile of MN frequency (> or = 12 MN/1000 cells) and those in the lowest quartile (< or = 4 MN/1000 cells) suggested an association between hair dye use and elevated MN frequency (OR 14.2 (95% CI 0.81-247.8; P=0.069)). None of the polymorphisms examined significantly modified association between hair dye use and frequency of MN. Findings of an increased frequency of MN in urothelial cells of hair dye users suggest a possible genotoxic effect of hair dye compounds and need confirmation in larger studies.
Espinoza
Felicidad
F
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Silverman
Debra
D
Kogevinas
Manolis
M
Creus
Amadeu
A
Fernández
Francisco
F
García-Closas
Montserrat
M
Tardon
Adonina
A
García-Closas
Reina
R
Serra
Consol
C
Carrato
Alfredo
A
Rothman
Nathaniel
N
Dosemeci
Mustafa
M
Malats
Nuria
N
Marcos
Ricard
R
D000368:Aged; D001191:Arylamine N-Acetyltransferase / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D006202:Hair Dyes / Q000633:toxicity*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D015162:Micronucleus Tests; D011110:Polymorphism, Genetic; D001749:Urinary Bladder Neoplasms / Q000235:genetics*; D019459:Urothelium / Q000166:cytology
D001191:Arylamine N-Acetyltransferase; D005982:Glutathione Transferase; D006202:Hair Dyes; D007527:Isoenzymes; C089384:N-acetyltransferase 1; C478900:NAT2 protein, human