A novel metallobridged bis(beta-cyclodextrin)s fluorescent probe for the determination of glutathione.
A novel metallobridged bis(beta-cyclodextrin)s 2 [bis(beta-CD)s 2] was synthesized and characterized by means of (1)H NMR, IR, element analysis and redox iodometric titration. The fluorescence of metallobridged bis(beta-CD)s 2 was weak compared with bis(beta-CD)s 1 because of the paramagnetism of copper (II) ions. Glutathione was able to form complexes with copper (II) derived from the metallobridged bis(beta-CD)s 2. This competitive complexation with copper (II) may lead to a significant fluorescence recovery of the bis(beta-CD)s. Therefore, a rapid and simple spectrofluorimetric method was developed for the determination of glutathione. The analytical application for glutathione was investigated in NaCl/P(i) (pH 6.00) at room temperature. The linear range of the method was 0.30-20.0 micromol.L(-1) with a detection limit of 63.8 nmol.L(-1). There was no interference from the plasma constituents. The proposed method had been successfully used to determine glutathione in human plasma.
Tang
Bo
B
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
Liu
Fang
F
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
Xu
Kehua
K
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
Tong
Lili
L
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, China.
D001952:Bridged-Ring Compounds / Q000138:chemical synthesis*; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005978:Glutathione / Q000032:analysis / Q000097:blood*; D006801:Humans; D015336:Molecular Probe Techniques; D009942:Organometallic Compounds / Q000138:chemical synthesis*; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000138:chemical synthesis*
D001952:Bridged-Ring Compounds; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D047392:beta-Cyclodextrins; D003300:Copper; D005978:Glutathione