Inhibition of glycolipid biosynthesis.
Adequate inhibition of glycolipid biosynthesis allows the study of their biological functions. The method presented in this unit employs a synthetic analog of ceramide, PDMP (1-phenyl-2-decanoylamino-3-morpholino-1-propanol), that inhibits glycolipid biosynthesis in cultured cells. Optimum conditions for inhibition of glycolipid biosynthesis are determined, glycolipids extracted from cultures grown with and without inhibitor, and the patterns of glycolipids analyzed by HPTLC. Detection is achieved using colorimetric reactions, or by monitoring radioactivity when cells have been metabolically radiolabeled.
Manzi
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AE
University of California, San Diego, La Jolla, California, USA.
D000818:Animals; D001345:Autoradiography; D002851:Chromatography, High Pressure Liquid; D006017:Glycolipids / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*
D006017:Glycolipids