Biotransformation of estrogens in nitrifying activated sludge under aerobic and alternating anoxic/aerobic conditions.
Natural and synthetic estrogens present in municipal wastewater can be biodegraded during treatment, particularly in activated sludge. The objective was to assess the extent of transformation of 17-beta-estradiol (E2) and 17-alpha-ethinylestradiol (EE2) by nitrifying activated sludge and evaluate potential relationships between availability of oxygen, nitrification rate, and estrogen removal. For each batch experiment, two reactors were set up--aerobic and alternating anoxic/aerobic-which were then amended with E2 and EE2 from methanolic stock solutions. The EE2 was persistent under anoxic conditions; under aerobic conditions, the observed level of its removal was 22%. The E2 was readily converted to estrone (El)--faster under aerobic (nitrifying) than anoxic (denitrifying) conditions. During the initial anoxic conditions, a metabolite consistent with 17-alpha-estradiol transiently accumulated and was subsequently removed when the reactor was aerated. Higher removal rates of estrogens were associated with higher nitrification rates, which supports the contention that the nitrifying biomass was responsible for their removal.
Dytczak
M A
MA
Department of Civil Engineering, University of Manitoba, Winnipeg, Canada.
Londry
K L
KL
Oleszkiewicz
J A
JA
D000332:Aerobiosis; D000693:Anaerobiosis; D001711:Biotransformation; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000302:isolation & purification / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D012722:Sewage / Q000737:chemistry*; D014865:Waste Disposal, Fluid / Q000379:methods*
D004967:Estrogens; D012722:Sewage; D004970:Estrone; D004997:Ethinyl Estradiol; D004958:Estradiol; D009584:Nitrogen