title: VEGF-enhanced proliferation under hypoxia by an autocrine mechanism in human vascular smooth muscle cells.
abstract: OBJECTIVE
  Atherosclerotic lesions are reported to be hypoxic. Since hypoxia is known to induce the production of growth factors, such as vascular endothelial growth factor (VEGF), we examined the implication of hypoxia-induced VEGF in the proliferation of human coronary artery smooth muscle cells (CASMCs).
  
  
  METHODS
  Cells were cultured under hypoxic conditions (1% O(2), 5% CO(2)) and several responses were measured.
  
  
  RESULTS
  Under hypoxic conditions, the mRNA and protein levels of VEGF, and the mRNA level of VEGF receptor-1 (VEGFR-1) increased with an increase in hypoxia-inducible factor-1alpha (HIF-1alpha) protein, and considerable amounts of VEGF were secreted. Hypoxia enhanced the incorporation of [(3)H]-thymidine by CASMCs, which was completely inhibited by a neutralizing antibody against VEGF. A neutralizing antibody against NADPH-cytochrome P-450 reductase (NPR), which contributes to the stabilization of HIF-1alpha, also attenuated hypoxia-stimulated proliferation. In NPR-knockdown cells, the expression of VEGF, proliferation, and transcriptional activity were attenuated, whereas in NPR-overexpressing cells, they were enhanced.
  
  
  CONCLUSIONS
  Hypoxia-induced proliferation of CASMCs is mediated through the expressions of VEGF and VEGFR-1 in an autocrine mechanism. Their expressions are dependent on the stabilization of HIF-1alpha, which is regulated by NPR. We suggest that hypoxia and hypoxia-induced VEGF expression are involved in the pathogenesis of progressive atherosclerosis.
authors.affiliation: Department of Pathological Biochemistry, Kyoto Pharmaceutical University, Kyoto, Japan. mayuko@mb.kyoto-phu.ac.jp
authors.forename: Mayuko
authors.initials: M
authors.lastname: Osada-Oka
authors.forename: Takako
authors.initials: T
authors.lastname: Ikeda
authors.forename: Susumu
authors.initials: S
authors.lastname: Imaoka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Akiba
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
mesh_terms: D019898:Autocrine Communication; D015153:Blotting, Western; D018929:Cell Culture Techniques; D003331:Coronary Vessels / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D006801:Humans; D000860:Hypoxia; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000503:physiopathology*; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; D040262:Receptors, Vascular Endothelial Growth Factor