15,16-dihydrotanshinone I, a major component from Salvia miltiorrhiza Bunge (Dansham), inhibits rabbit platelet aggregation by suppressing intracellular calcium mobilization.
Radix Salviae miltiorrhiza (RSM, 'Dansham' in Korea, 'Danshen' in Chinese), the root of Salviae miltiorrhiza Bunge (Labiate) has been used as Chinese fork medicine for the treatment of cardiovascular diseases such as angina pectoris, coronary heart disease, myocardial infarction, and hypertension. In the present study, we evaluated the inhibitory effects of 15,16-Dihydrotanshinone I, one of the major ingredients of Salvia miltiorrhiza Bunge, on platelet aggregation, with elucidation of its mechanisms of action. 15,16-Dihydrotanshinone I concentration-dependently inhibited collagen-induced aggregation of rabbit washed platelets with IC50 of 8.7+/-5.6 microM, the potency being about seven-fold greater than EGCG, an active Green tea catechin component (IC50: 56.6+/-48.7 microM). 15,16-Dihydrotanshinone I significantly inhibited the intracellular calcium ([Ca2+]i) mobilization in a concentration-dependent manner. 15,16-dihdydrotanshinone I also significantly suppressed collagen (50 microg/mL)-induced liberation of [3H]Arachidonic acid from [3H]Arachidonic acid-incorporated rabbit platelet. In addition, 15,16-Dihydrotanshinone I at 50 microM slightly but significantly inhibited collagen-induced production of thromboxane B2. These results indicate that 15,16-Dihydrotanshinone I exert potent anti-platelet activity via suppression of [Ca2+]i mobilization and arachidonic acid liberation.
Park
Jung-Woo
JW
Department of Applied Biochemistry, Division of Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea.
Lee
Sung-Hun
SH
Yang
Min-Kyu
MK
Lee
Jung-Jin
JJ
Song
Min-Jin
MJ
Ryu
Si-Yong
SY
Chung
Hun-Jong
HJ
Won
Hyung-Sik
HS
Lee
Chang-Soo
CS
Kwon
Suk-Hyung
SH
Yun
Yeo-Pyo
YP
Choi
Wahn-Soo
WS
Shin
Hwa-Sup
HS
D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D003094:Collagen / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005663:Furans; D066298:In Vitro Techniques; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors; D011809:Quinones; D011817:Rabbits; D027544:Salvia / Q000737:chemistry*; D013917:Thrombin / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood
D005663:Furans; D010616:Phenanthrenes; D010975:Platelet Aggregation Inhibitors; D011809:Quinones; D016718:Arachidonic Acid; D013929:Thromboxane B2; C000713095:dihydrotanshinone I; D003094:Collagen; D013917:Thrombin; D002118:Calcium