Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer.
A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.
Stevenson
Ross
R
School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, Scotland EH9 3JJ, UK.
Baxter
Helen C
HC
Aitken
Alastair
A
Brown
Tom
T
Baxter
Robert L
RL
D048948:14-3-3 Proteins / Q000134:cerebrospinal fluid / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D001483:Base Sequence; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D052156:SELEX Aptamer Technique; D012608:Scrapie / Q000635:transmission; D012756:Sheep
D048948:14-3-3 Proteins; D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D011994:Recombinant Proteins; D004247:DNA