A method for the rapid isolation of virus from cultured cells.
A simple and efficient collection method using hypotonic burst to isolate virions from infected cultured cells is described. Distilled water treatment of avian metapneumovirus (AMPV)-infected cells with thorough mixing and repeated pipeting was considerably faster for virion collection in avian cells compared to the widely used freeze-thaw (F-T) method (30 min vs. 3-4 h). This method was also more effective for virion collection. The total number of virions recovered from AMPV-infected immortal turkey turbinate cells by the novel water lysis method was 3-fold higher than by the F-T method. This simple water lysis method can be applied to virion collection for other RNA viruses such as the paramyxoviruses that are used to infect cultured cells.
Kong
Byung-Whi
BW
Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, USA.
Foster
Linda K
LK
Foster
Douglas N
DN
D000818:Animals; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D005615:Freezing; D007038:Hypotonic Solutions; D029121:Metapneumovirus / Q000302:isolation & purification*; D014709:Vero Cells; D014771:Virion / Q000302:isolation & purification; D014867:Water
D007038:Hypotonic Solutions; D014867:Water