title: Promotion of immunity of mice to Pasteurella multocida and hog cholera vaccine by pig interleukin-6 gene and CpG motifs.
abstract: A novel oligodeoxynuleotides containing 11 CpG motifs was synthesized and inserted into the VR1020 plasmid containing pig interleukin-6 (IL-6) gene (VPIL6) to construct recombinant plasmid, VPIL6C. The chitosan nanoparticles (CNP) were prepared by ionic cross linkage to entrap the VPIL6C (VPIL6C-CNP), VPIL6 (VPIL6-CNP) and CpG (CpG-CNP). 42-Day old female mice were divided into four groups and intramuscularly injected respectively with 6 pmol VPIL6C-CNP, VPIL6-CNP, CpG-CNP and VR1020-CNP along with the bivalent vaccines against the Pasteurellosis and hog cholera. The blood was weekly collected from mice after vaccination to detect the changes of immunoglobulins, specific antibodies, IL-2, IL-4, IL-6 and immune cells. 28 days after vaccination, the mice were orally challenged with virulent Pasteurella multocida. The results showed that in comparison with those of the control VR1020 group, the content of immunoglobulins, specific antibodies and interleukins significantly increased in the sera from the treated two groups (P<0.05). Meanwhile, the number of lymphocytes and monocytes also remarkably elevated in the treated groups (P<0.05). The immune responses of VPIL6C mice were notably stronger than those of VPIL6 and CpG group. The challenge results proved that the overall immunity was further promoted in the treated mice which resisted the challenge infection; while the control mice manifested evident symptoms and lesions, and died of infection. These suggested that VPIL6C-CNP could better promote the immunity and resistance of mice against Pasteurellosis than VPIL6-CNP and CpG-CNP, and facilitate the development of effective adjuvant to enhance the immunity of animal against infection.
authors.affiliation: Key Laboratory for Bio-Resource and Eco-Environment of Ministry Education, Bioengineering Research Center for Animal Disease Prevention and Control, Life Science College, Sichuan University, Sichuan, PR China.
authors.forename: Zhong-Zhong
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Hua-Bing
authors.initials: HB
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xie
authors.forename: Ying-Yu
authors.initials: YY
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Ze-Zhou
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Jiang-Ling
authors.initials: JL
authors.lastname: Li
authors.forename: Kai-Yuan
authors.initials: KY
authors.lastname: Wu
authors.forename: Hong-Ning
authors.initials: HN
authors.lastname: Wang
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Meng
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D048271:Chitosan / Q000008:administration & dosage; D006691:Classical Swine Fever / Q000517:prevention & control*; D006692:Classical Swine Fever Virus / Q000276:immunology; D005260:Female; D007136:Immunoglobulins / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000276:immunology*; D010326:Pasteurella Infections / Q000382:microbiology / Q000517:prevention & control*; D016979:Pasteurella multocida / Q000276:immunology*; D010957:Plasmids / Q000235:genetics / Q000276:immunology; D013552:Swine / Q000276:immunology / Q000821:virology; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; C408982:CPG-oligonucleotide; D007136:Immunoglobulins; D007376:Interleukin-2; D015850:Interleukin-6; D009838:Oligodeoxyribonucleotides; D019444:Vaccines, DNA; D014765:Viral Vaccines; D015847:Interleukin-4; D048271:Chitosan