title: Development of R4 gateway binary vectors (R4pGWB) enabling high-throughput promoter swapping for plant research.
abstract: We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction of chimeric genes between any promoter and any cDNA. The structure of R4pGWBs is almost the same as the promoterless type of improved pGWBs (ImpGWBs), except that the attR1 site is replaced with attR4, which enables tripartite recombination of these vectors with promoter- and cDNA-entry clones. While ImpGWBs are suitable for promoter analysis and constitutive expression of cDNAs in higher plants, R4pGWBs have a great advantage in expressing a cDNA under the regulation of desired promoters.
authors.affiliation: Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue 690-8504, Japan. tnakagaw@life.shimane-u.ac.jp
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Shinya
authors.initials: S
authors.lastname: Nakamura
authors.forename: Katsunori
authors.initials: K
authors.lastname: Tanaka
authors.forename: Makoto
authors.initials: M
authors.lastname: Kawamukai
authors.forename: Takamasa
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kenzo
authors.initials: K
authors.lastname: Nakamura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kimura
authors.forename: Sumie
authors.initials: S
authors.lastname: Ishiguro
mesh_terms: D018076:DNA, Complementary; D005822:Genetic Vectors; D010944:Plants / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D018076:DNA, Complementary