Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos.
The monoclonal antibody 6E2 raised against the embryonal carcinoma cell line NCR-G3 had been shown to also react with human germ cells. Thin-layer chromatography (TLC) immunostaining revealed that 6E2 specifically reacts with sialosylglobopenta osylceramide (sialylGb5), which carries an epitope of stage-specific embryonic antigen-4 (SSEA-4), known as an important cell surface marker of embryogenesis. The immunostaining of mouse preimplantation embryos without fixation showed that the binding of 6E2 caused the clustering and consequent accumulation of sialylGb5 at the interface between blastomeres. These results suggest that SSEA-4 actively moves on the cell surface and readily accumulates between blastomeres after binding of 6E2.
Sato
Ban
B
Department of Developmental Biology, National Research Institute for Child Health and Development, 2-1 0-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.
Katagiri
Yohko U
YU
Miyado
Kenji
K
Akutsu
Hidenori
H
Miyagawa
Yoshitaka
Y
Horiuchi
Yasuomi
Y
Nakajima
Hideki
H
Okita
Hajime
H
Umezawa
Akihiro
A
Hata
Jun-Ichi
J
Fujimoto
Junichiro
J
Toshimori
Kiyotaka
K
Kiyokawa
Nobutaka
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism*; D001757:Blastomeres / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006028:Glycosphingolipids / Q000378:metabolism*; D000882:Haplorhini; D006801:Humans; D008297:Male; D051379:Mice; D055746:Stage-Specific Embryonic Antigens
D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4