title: C-reactive protein (CRP) increases VEGF-A expression in monocytic cells via a PI3-kinase and ERK 1/2 signaling dependent pathway.
abstract: C-reactive protein (CRP) is an independent predictor of atherosclerosis and its complications. Monocytes/macrophages are implicated in this complex disease which is, among other mechanisms, characterised by angiogenesis. The aim of this study was to analyse whether CRP plays a role in VEGF-A regulation by monocytic cells. Our findings show that CRP up-regulates VEGF-A mRNA expression and protein excretion in THP-1 cells in a concentration- and time-dependent manner. Furthermore, we studied the signaling pathway underlying this effect. CRP increases VEGF-A expression via a PI3-kinase and an extracellular-signal-regulated kinase (ERK) 1/2 dependent pathway. Our results suggest that CRP could play a role in the angiogenesis process via immune cells such as monocytes.
authors.affiliation: INSERM, U684, Vandoeuvre les Nancy, F-54500 France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Bello
authors.forename: Frédéric
authors.initials: F
authors.lastname: Cailotto
authors.forename: Didier
authors.initials: D
authors.lastname: Hanriot
authors.forename: Marie-Nathalie
authors.initials: MN
authors.lastname: Kolopp-Sarda
authors.forename: Véronique
authors.initials: V
authors.lastname: Latger-Cannard
authors.forename: Ketsia
authors.initials: K
authors.lastname: Hess
authors.forename: Faiez
authors.initials: F
authors.lastname: Zannad
authors.forename: Dan
authors.initials: D
authors.lastname: Longrois
authors.forename: Armelle
authors.initials: A
authors.lastname: Ropars
mesh_terms: D002097:C-Reactive Protein / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D020935:MAP Kinase Signaling System; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D002097:C-Reactive Protein; D019869:Phosphatidylinositol 3-Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D048051:p38 Mitogen-Activated Protein Kinases