Differentiation of the traditional Chinese medicinal plants Euphorbia humifusa and E. maculata from adulterants by TaqMan real-time polymerase chain reaction.
DNA sequence analysis of the rDNA internal transcribed spacer 1 (ITS1) and TaqMan real-time polymerase chain reaction were exploited for their applications in the differentiation of the traditional chinese medicinal plants euphorbia humifusa and e. maculata from three related adulterants e. hypericifolia, E. atoto and E. prostrata. The data demonstrated that variations in the ITS1 regions were very low at the intra-species level but extremely high at the inter-species level, so that they could be easily distinguished at the DNA level. The sequence difference allowed an effective and reliable differentiation of E. humifusa and E. maculata from the adulterants by TaqMan real-time PCR.
Xue
Heng-Gang
HG
People's Liberation Army General Hospital, Beijing, People's Republic of China.
Wang
Hong
H
Li
De-Zhu
DZ
Xue
Chun-Ying
CY
Wang
Qing-Zhong
QZ
D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal / Q000145:classification; D028481:Euphorbia / Q000145:classification* / Q000235:genetics; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction
D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal