title: [Relationship between gastrointestinal dyskinesis and gastrointestinal neurons in diabetic mellitus: experiment with rats].
abstract: OBJECTIVE
  To investigate the relationship between gastrointestinal dyskinesis and histologic changes of gastrointestinal myenteric plexus cholinergic and nitrergic neurons in STZ-induced diabetic rats.
  
  
  METHODS
  45 SD rats were randomly divided into control group, diabetic group and insulin group. 16 weeks after diabetic model established, gastrointestinal motility of rats was measured and histologic changes of myenteric plexus cholinergic neuron and nitrergic neuron was observed.
  
  
  RESULTS
  Compared with control group, gastrointestinal motility of diabetic group was markedly slow (P < 0.01), the myenteric plexus cholinergic neuron counting of gastric antrum and small intestine were significantly decreased (6.6 +/- 2.9 vs 15.7 +/- 3.8 15.6 +/- 10.3 vs 22.6 +/- 7.4, P < 0.01), yet the number of nitrergic neuron only markedly reduced in gastric antrum (5.3 +/- 1.2 vs 11.8 +/- 2.2, P < 0.01). The gastrointestinal mobility, gastric antrum nitrergic neuron and small intestine cholinergic neuron counting of insulin group were markedly higher than that of diabetic group (P < 0.05), yet lower than that of control group (P < 0.01).
  
  
  CONCLUSIONS
  The gastrointestinal dyskinesis of STZ-induced diabetic rats might be associated with lesions of gastrointestinal myenteric plexus cholinergic neuron and nitrergic neuron. Insulin intensive therapy can partly ameliorate diabetic gastrointesternal dyskinesis.
authors.affiliation: Department of Endocrinology, General Hospital, Tianjin Medical College, Tianjin, China.
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Hou
authors.forename: Chao-Xun
authors.initials: CX
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Luo
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Jia
authors.forename: Ming-Cai
authors.initials: MC
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002799:Cholinergic Fibers / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005769:Gastrointestinal Motility / Q000502:physiology; D041981:Gastrointestinal Tract / Q000503:physiopathology*; D006651:Histocytochemistry; D007421:Intestine, Small / Q000294:innervation / Q000503:physiopathology; D008297:Male; D026602:Nitrergic Neurons / Q000502:physiology*; D011706:Pyloric Antrum / Q000294:innervation / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley