What makes centromeric cohesion resistant to separase cleavage during meiosis I but not during meiosis II?
Segregation of chromosomes during meiosis I is triggered by separase cleavage of the cohesin's Rec8 subunit along chromosome arms. Centromeric cohesin is protected from separase cleavage during meiosis I by Sgo1/MEI-S332 proteins in complex with protein phosphatase 2A (PP2A). This retention of centromeric sister chromatid cohesion is essential for faithful segregation of chromatids during the second meiotic division. While Sgo1/PP2A complex is required for protecting centromeric sister chromatid cohesion during meiosis I, it is not known what renders the centromeric cohesion sensitive to separase cleavage during meiosis II. Our data suggest that the absence of Sgo1 and PP2A from meiosis II centromeres is not sufficient to render centromeric cohesion sensitive to cleavage by separase and additional factors are required to ensure the removal of centromeric cohesion during meiosis II.
Gregan
Juraj
J
Max F. Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria. juraj.gregan@univie.ac.at
Rumpf
Cornelia
C
Li
Zhang
Z
Cipak
Lubos
L
D020413:3' Untranslated Regions / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002503:Centromere / Q000737:chemistry / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D020090:Chromosome Segregation; D010450:Endopeptidases / Q000378:metabolism*; D008540:Meiosis; D009687:Nuclear Proteins / Q000378:metabolism; D054648:Protein Phosphatase 2 / Q000378:metabolism; D012568:Schizosaccharomyces / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D064247:Separase; D000097722:Cohesins
D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D010450:Endopeptidases; D009687:Nuclear Proteins; D054648:Protein Phosphatase 2; D029702:Schizosaccharomyces pombe Proteins; D064247:Separase; D000097722:Cohesins; C485973:Sgo1 protein, S pombe