title: Mechanisms related to the genotoxicity of particles in the subway and from other sources.
abstract: Negative health effects of airborne particles have clearly been shown in epidemiological studies. People get exposed to particles from various sources such as the combustion of, for example, diesel and wood and also from particles arising from tire-road wear. Another source of importance for certain populations is exposure to particles in subway systems. We recently reported that these particles were more genotoxic when compared to that of several other particle types. The aim of this study was to further investigate and compare the toxicity of subway particles and particles from other sources as well as investigate some mechanisms behind the genotoxicity of subway particles. This was done by comparing the ability of subway particles and particles from a street, pure tire-road wear particles, and particles from wood and diesel combustion to cause mitochondrial depolarization and to form intracellular reactive oxygen species (ROS). Furthermore, the genotoxicity and ability to cause oxidative stress was compared to magnetite particles since this is a main component in subway particles. It was concluded that the subway particles and also street particles and particles from wood and diesel combustion caused mitochondrial depolarization. The ability to damage the mitochondria is thus not the only explanation for the high genotoxicity of subway particles. Subway particles also formed intracellular ROS. This effect may be part of the explanation as to why subway particles show such high genotoxicity when compared to that of other particles. Genotoxicity can, however, not be explained by the main component, magnetite, by water-soluble metals, or by intracellular mobilized iron. The genotoxicity is most likely caused by highly reactive surfaces giving rise to oxidative stress.
authors.affiliation: Unit for Analytical Toxicology, Department of Biosciences and Nutrition at Novum, Karolinska Institutet, SE-141 57 Huddinge, Stockholm, Sweden.
authors.forename: Hanna L
authors.initials: HL
authors.lastname: Karlsson
authors.forename: Asa
authors.initials: A
authors.lastname: Holgersson
authors.forename: Lennart
authors.initials: L
authors.lastname: Möller
mesh_terms: D000395:Air Pollutants, Occupational / Q000633:toxicity*; D002460:Cell Line; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D052203:Ferrosoferric Oxide / Q000633:toxicity; D005374:Filtration; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000633:toxicity; D008670:Metals / Q000737:chemistry / Q000633:toxicity; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity*; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D011890:Railroads; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000395:Air Pollutants, Occupational; D008670:Metals; D009153:Mutagens; D052638:Particulate Matter; D017382:Reactive Oxygen Species; D007501:Iron; D052203:Ferrosoferric Oxide