title: Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy.
abstract: This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L(-1) culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.
authors.affiliation: Department of Analytical Chemistry, Ghent University, Proeftuinstraat 86, 9000 Ghent, Belgium. Raman@UGhent.be
authors.forename: Joke De
authors.initials: JD
authors.lastname: Gelder
authors.forename: Diana
authors.initials: D
authors.lastname: Willemse-Erix
authors.forename: Maarten J
authors.initials: MJ
authors.lastname: Scholtes
authors.forename: Jorge I
authors.initials: JI
authors.lastname: Sanchez
authors.forename: Kees
authors.initials: K
authors.lastname: Maquelin
authors.forename: Peter
authors.initials: P
authors.lastname: Vandenabeele
authors.forename: Patrick De
authors.initials: PD
authors.lastname: Boever
authors.forename: Gerwin J
authors.initials: GJ
authors.lastname: Puppels
authors.forename: Luc
authors.initials: L
authors.lastname: Moens
authors.forename: Paul De
authors.initials: PD
authors.lastname: Vos