Microelectrode array (MEA) platform for targeted neuronal transfection and recording.
Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.
Jain
Tilak
T
Department of Bioengineering, Arizona State University, Tempe, AZ 85287 USA.
Muthuswamy
Jit
J
D000818:Animals; D018929:Cell Culture Techniques; D018274:Electroporation; D015316:Genetic Therapy / Q000379:methods; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D040081:Protein Array Analysis; D034741:RNA, Small Interfering; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
D034741:RNA, Small Interfering