In vitro propagation and chemical and biological studies of the essential oil of Salvia przewalskii Maxim.
The procedure of Salvia przewalskii shoot multiplication and the ability of regenerated plants to produce essential oil is reported. The essential oil was obtained by hydrodistillation from leaves and flowering stems of field-grown plants, and their chemical composition was examined by GC, GC-MS and 1H NMR. The differences in yield as well as qualitative and quantitative composition between the oils isolated from in vitro and in vivo plants were observed. S. przewalskii essential oil was tested for its antimicrobial and cytotoxic properties. It was found that cytotoxicity against human leukemia HL-60 cells and antimicrobial activity (especially, against Staphylococcus aureus and S. epidermidis strains) of oils isolated from in vitro plants were higher than those for oils from in vivo S. przewalskii plants.
Skala
Ewa
E
Department of Biology and Pharmaceutical Botany, Medical University of Łódz, Poland. botanika@pharm.am.lodz.pl
Kalemba
Danuta
D
Wajs
Anna
A
Rózalski
Marek
M
Krajewska
Urszula
U
Rózalska
Barbara
B
Wieckowska-Szakiel
Marzena
M
Wysokińska
Halina
H
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002849:Chromatography, Gas; D035264:Flowers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000302:isolation & purification* / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*
D000970:Antineoplastic Agents; D009822:Oils, Volatile; D010938:Plant Oils