Highlights of glucosamine-6P synthase catalysis.
L-Glutamine:d-fructose-6-phosphate amidotransferase, also known as glucosamine-6-phosphate synthase (GlcN6P synthase), which catalyzes the first step in a pathway leading to the formation of uridine 5'-diphospho-N-acetyl-d-glucosamine (UDP-GlcNAc), is a key point in the metabolic control of the biosynthesis of amino sugar-containing macromolecules. The molecular mechanism of the reaction catalyzed by GlcN6P synthase is complex and involves amide bond cleavage followed by ammonia channeling and sugar isomerization. This article provides a comprehensive overview of the present knowledge on this multi-faceted enzyme emphasizing the progress made during the last five years.
Durand
Philippe
P
Institut de Chimie des Substances Naturelles-CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
Golinelli-Pimpaneau
Béatrice
B
Mouilleron
Stéphane
S
Badet
Bernard
B
Badet-Denisot
Marie-Ange
MA
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002176:Candida albicans / Q000201:enzymology; D002384:Catalysis; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007536:Isomerism; D007700:Kinetics; D008958:Models, Molecular; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*
D001426:Bacterial Proteins; D005656:Fungal Proteins; D014537:Uridine Diphosphate N-Acetylglucosamine; D000641:Ammonia; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D005947:Glucose