Surface localization of the nuclear receptor CAR in influenza A virus-infected cells.
Constitutive active/androstane receptor CAR is a member of the nuclear receptors which regulate transcription of xenobiotic metabolism enzymes. CAR is usually localized in the cytosol and nucleus. Here, we found that CAR was localized at the cell surface of influenza A virus (IAV)-infected cells. Additionally, we demonstrated that expression of a viral envelope glycoprotein, either hemagglutinin (HA) or neuraminidase (NA), but not viral nucleoprotein (NP), was responsible for this localization. This report is the first demonstration of CAR at the surface of tissue culture cells, and suggests that CAR may exert the IAV infection mechanism.
Takahashi
Tadanobu
T
Department of Pharmaco-Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, and COE Program in the 21st Century, 52-1 Yada, Shizuoka 422-8526, Japan.
Moriyama
Yusuke
Y
Ikari
Akira
A
Sugatani
Junko
J
Suzuki
Takashi
T
Miwa
Masao
M
D002462:Cell Membrane / Q000378:metabolism* / Q000821:virology*; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism*; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology*; D011991:Receptors, Virus / Q000378:metabolism*
C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011991:Receptors, Virus