Ribosomal proteins are targets for the NEDD8 pathway.
Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.
Xirodimas
Dimitris P
DP
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK. d.xirodimas@dundee.ac.uk
Sundqvist
Anders
A
Nakamura
Akihiro
A
Shen
Linnan
L
Botting
Catherine
C
Hay
Ronald T
RT
D010450:Endopeptidases / Q000378:metabolism; D006207:Half-Life; D006367:HeLa Cells; D006801:Humans; D000075687:NEDD8 Protein; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D014162:Transfection; D014452:Ubiquitins / Q000378:metabolism*
D000075687:NEDD8 Protein; C082860:NEDD8 protein, human; D012269:Ribosomal Proteins; D014452:Ubiquitins; D010450:Endopeptidases; C477008:SENP8 protein, human