Evidence of a continuous endoplasmic reticulum in the protozoan parasite Entamoeba histolytica.
Entamoeba histolytica, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles. Here, using an ER-targeted green fluorescent protein fusion protein and fluorescence loss in photobleaching, we have unambiguously demonstrated the presence of a continuous ER compartment in living E. histolytica trophozoites.
Teixeira
Jose E
JE
Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05405, USA.
Huston
Christopher D
CD
D000818:Animals; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D004749:Entamoebiasis / Q000469:parasitology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D038761:Photobleaching; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins