Human somatic cell gene targeting.
Human somatic cell gene targeting provides a powerful tool to scientists studying gene function in cultured human cells. This technology allows scientists to knock out genes in human somatic cells in a fashion analogous to the creation of knockout mice. Human somatic cell gene targeting brings the power of genetics to the study of human genes in human cells by making it possible to compare cells or individuals that are genetically identical except for a single, well-defined mutation in an endogenous gene. These modified cells can be studied both in vitro and in vivo. This unit presents protocols for human somatic cell gene targeting.
Waldman
Todd
T
Georgetown University School of Medicine, Washington, D.C, USA.
Lee
Carolyn
C
Nishanian
Tagvor G
TG
Kim
Jung-Sik
JS
D000483:Alleles; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D004351:Drug Resistance / Q000235:genetics; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D006579:Heterozygote; D006801:Humans; D019426:Integrases / Q000378:metabolism; D019868:Kanamycin Kinase / Q000235:genetics; D014162:Transfection / Q000379:methods
D005839:Gentamicins; C010680:antibiotic G 418; D019868:Kanamycin Kinase; C045073:Cre recombinase; D019426:Integrases