GD3 nuclear localization after apoptosis induction in HUT-78 cells.
Glycosphingolipids are essential components of plasma membrane and act as antigens, mediators of cell adhesion, and modulators of signal transduction. Following activation of the Fas receptor, gangliosides are recuited in various intracellular compartments. We have evaluated whether the pro-apoptotic anti-CD95 antibody induces a nuclear localization of GD3 in HUT-78 cells. Our data shows that GD3 translocation from cytosol to nuclei is strongly correlated to concomitant rapid phosphorylation of histone H1 shortly after induction of apoptosis. This work advances the hypothesis that GD3 induces a post-translational modification of histone H1 thus influencing the apoptosis process through transcriptional activation/repression of specific genes.
Tempera
Italo
I
Department of Biochemical Sciences, University "La Sapienza", p.le A. Moro, 5, 00185 Roma, Italy.
Buchetti
Barbara
B
Lococo
Emanuela
E
Gradini
Roberto
R
Mastronardi
Annalisa
A
Mascellino
Maria Teresa
MT
Sale
Patrizio
P
Mosca
Luciana
L
d'Erme
Maria
M
Lenti
Luisa
L
D021581:Active Transport, Cell Nucleus / Q000187:drug effects / Q000502:physiology*; D000906:Antibodies / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D005732:Gangliosides / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D019014:fas Receptor / Q000276:immunology
D000906:Antibodies; D005732:Gangliosides; D006657:Histones; D019014:fas Receptor; C026226:ganglioside, GD3