HPLC methods for the fractionation and analysis of negatively charged oligosaccharides and gangliosides.
This unit describes the fractionation and analysis of anionic oligosaccharides and gangliosides using anion-exchange high-performance liquid chromatography (HPLC). Saccharides or gangliosides are eluted in order of the number of negative charges they possess, although the charge-to-mass ratio can also contribute to elution position.
Manzi
A E
AE
University of California San Diego, La Jolla, California, USA.
Hayes
B K
BK
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D007477:Ions / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry*; D013997:Time Factors
D005732:Gangliosides; D007477:Ions; D009844:Oligosaccharides