Chromatin assembly using Drosophila systems.
To successfully study chromatin structure and activity in vitro, it is essential to have a chromatin assembly system that will prepare extended nucleosome arrays with highly defined protein content that resemble bulk chromatin isolated from living cell nuclei in terms of periodicity and nucleosome positioning. The Drosophila ATP-dependent chromatin assembly system described in this unit meets these requirements. The end product of the reaction described here has highly periodic extended arrays with physiologic spacing and positioning of the nucleosomes.
Fyodorov
Dmitry V
DV
University of California, San Diego, La Jolla, California, USA.
Levenstein
Mark E
ME
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D020134:Catalytic Domain; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology; D004247:DNA / Q000302:isolation & purification / Q000502:physiology; D004264:DNA Topoisomerases, Type I / Q000737:chemistry / Q000302:isolation & purification; D004270:DNA, Circular / Q000737:chemistry; D029721:Drosophila Proteins / Q000302:isolation & purification / Q000502:physiology; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000737:chemistry; D005822:Genetic Vectors / Q000235:genetics; D006657:Histones / Q000302:isolation & purification / Q000502:physiology; D007202:Indicators and Reagents; D018411:Spodoptera
D002457:Cell Extracts; D004270:DNA, Circular; D029721:Drosophila Proteins; D006657:Histones; D007202:Indicators and Reagents; D004247:DNA; D004264:DNA Topoisomerases, Type I