Metabolic labeling with amino acids.
Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This update focuses on pulse-labeling and pulse-chase experiments done with [(35)S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.
Bonifacino
J S
JS
National Institute of Child Health and Human Development, Bethesda, Maryland, USA.
D000596:Amino Acids; D000818:Animals; D002478:Cells, Cultured; D011232:Chemical Precipitation; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008715:Methionine; D011506:Proteins / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013997:Time Factors; D014238:Trichloroacetic Acid
D000596:Amino Acids; D011506:Proteins; D013462:Sulfur Radioisotopes; D014238:Trichloroacetic Acid; D008715:Methionine