title: Biokinetic parameters and behavior of Aeromonas hydrophila during anaerobic growth.
abstract: The biokinetics of glucose metabolism were evaluated in Aeromonas hydrophila during growth in an anaerobic biosystem. After approx 34 h growth, A. hydrophila metabolized 5,000 mg glucose l(-1) into the end-products ethanol, acetate, succinate and formate. The maximum growth rate, mu(m), half saturation coefficients, K(s), microbial yield coefficient, Y, cell mass decay rate coefficient, k(d), and substrate inhibition coefficient, K(si) were 0.25 +/- 0.03 h(-1), 118 +/- 31 mg glucose l(-1), 0.12 microg DNA mg glucose(-1), 0.01 h(-1), and 3,108 +/- 1,152 mg glucose l(-1), respectively. These data were used to predict the performance of a continuous growth system with an influent glucose concentration of 5,000 mg l(-1). Results of the analysis suggest that A. hydrophila will metabolize glucose at greater than 95% efficiency when hydraulic retention times (HRTs) exceed 7 h, whereas the culture is at risk of washing out at an HRT of 6.7 h.
authors.affiliation: School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk, 790-784, Korea.
authors.forename: Seungyeop
authors.initials: S
authors.lastname: Lee
authors.forename: Jaai
authors.initials: J
authors.lastname: Kim
authors.forename: Seung Gu
authors.initials: SG
authors.lastname: Shin
authors.forename: Seokhwan
authors.initials: S
authors.lastname: Hwang
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D016980:Aeromonas hydrophila / Q000254:growth & development* / Q000378:metabolism*; D000693:Anaerobiosis; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D000431:Ethanol / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis; D005285:Fermentation; D005561:Formates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005232:Fatty Acids, Volatile; D005561:Formates; C030544:formic acid; D000431:Ethanol; D019802:Succinic Acid; D005947:Glucose; D019342:Acetic Acid