title: Evidence of a continuous endoplasmic reticulum in the protozoan parasite Entamoeba histolytica.
abstract: Entamoeba histolytica, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (ER), with ER functions occurring in vesicles. Here, using an ER-targeted green fluorescent protein fusion protein and fluorescence loss in photobleaching, we have unambiguously demonstrated the presence of a continuous ER compartment in living E. histolytica trophozoites.
authors.affiliation: Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05405, USA.
authors.forename: Jose E
authors.initials: JE
authors.lastname: Teixeira
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Huston
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D004749:Entamoebiasis / Q000469:parasitology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D038761:Photobleaching; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins