title: [Effects of "surrounding needling" on hydroxyproline content and ultrastructures in the dermis of aged rats].
abstract: OBJECTIVE
  To probe the mechanism of acupuncture in anti-skin aging.
  
  
  METHODS
  A blank control group, an operation control group, an 8-month young group and an 18-month aged group were set up. Soluble and total hydroxyproline contents of the abdominal skin were compared, and appearance of fibroblast and arrangement of collagenous fibers were observed with transmission electron microscope, effects of surrounding needling on contents of collagenous fibers and ultrastructures of the dermis of the aged rats were observed, and the results were compared with results reported.
  
  
  RESULTS
  After surrounding needling, soluble hydroxyproline content of the skin significantly increased and the total hydroxyproline content did not change in the rats as compared with the aged control group; and cellular organs in dermis fibroblast decreased and cellular structures retrograded, and the space between collagenous fibers widened, and aging frame-bridge increased in the aged rats, but after surrounding needling fibroblast activity strengthened.
  
  
  CONCLUSIONS
  Surrounding needling can change aging state of skin possibly by strengthening the activity of fibroblast in skin and increasing the content of soluble collagen.
authors.affiliation: Affiliated Hospital of Liaoning University of TCM, Shenyang 110032, China. tcm-luyuan@hotmail.com
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D003094:Collagen / Q000032:analysis; D005260:Female; D006909:Hydroxyproline / Q000032:analysis*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry* / Q000648:ultrastructure*; D015595:Skin Aging
chemical_list: D003094:Collagen; D006909:Hydroxyproline