Quantitative chiral analysis of salsolinol in different brain regions of rats genetically predisposed to alcoholism.
A method to determine the catecholamine content in putamen (CPU) and midbrain (MB) regions of the brain of alcohol-preferring rats (P) is presented with a focus on the low-level detection of S,R-salsolinol, a metabolite of dopamine and a putative alcoholism marker. The developed strategy allows both quantitative profiling of related catecholamines and the enantiomeric separation and quantification of the S- and R-salsolinol isomers and their ratios. The described LC/MS strategy simplifies the current methodology that typically employs GC-MS by eliminating the need for derivatization. The data also suggest an increase in the non-enzymatic formation of salsolinol as a consequence of ethanol exposure.
Rojkovicova
Tatiana
T
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Mechref
Yehia
Y
Starkey
Jason A
JA
Wu
Guangxiang
G
Bell
Richard L
RL
McBride
William J
WJ
Novotny
Milos V
MV
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry; D002482:Cellulose / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D003505:Cyclodextrins / Q000737:chemistry; D004298:Dopamine / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D007546:Isoquinolines / Q000032:analysis* / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008636:Mesencephalon / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D011699:Putamen / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D003505:Cyclodextrins; D007546:Isoquinolines; C041953:cyclodextrin polymer; D002482:Cellulose; C036617:salsolinol; D004298:Dopamine