Purification of immunoglobulin G fraction from antiserum, ascites fluid, or hybridoma supernatant.
This unit describes the isolation of the immunoglobulin G (IgG) fraction (containing antibodies of all specificities) from a complex protein mixture such as antiserum, ascites fluid, or hybridoma supernatant. The basic procedure utilizes saturated ammonium sulfate solution to precipitate the IgG fraction, while an alternate protocol describes fractionation of IgG by chromatography on DEAE-Affi-Gel Blue resin.
Cooper
H M
HM
Walter and Eliza Hall Institute, Melbourne, Australia.
Paterson
Y
Y
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology*; D005591:Chemical Fractionation; D002845:Chromatography; D003470:Culture Media / Q000737:chemistry*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D044967:Serum / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007074:Immunoglobulin G