title: Rapid quantification of total genomic DNA methylation degree by short-end injection capillary zone electrophoresis.
abstract: We propose a new capillary zone electrophoresis method applying short-end injection technique for the fast evaluation of methylcystosine/total cytosine ratio after acidic DNA hydrolysis. By short-end injection and by using a 100 mmol/l Tris solution titrated with 1 mol/l phosphoric acid to pH 3.75 as background electrolyte, cytosine and methylcytosine were separated with a good resolution in less than 1.5 min. Stepwise multiple linear regression with DNA methylation degree as the dependent variable and age, cysteine, homocysteine and methionine as independent variables, showed a negative association with age and that total cysteine is the most important determinant of DNA methylation.
authors.affiliation: Department of Biomedical Sciences, Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43, Sassari, Italy. salvatore.sotgia@gmail.com
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sotgia
authors.forename: Ciriaco
authors.initials: C
authors.lastname: Carru
authors.forename: Flavia
authors.initials: F
authors.lastname: Franconi
authors.forename: Paolo Battista
authors.initials: PB
authors.lastname: Fiori
authors.forename: Salvatorica
authors.initials: S
authors.lastname: Manca
authors.forename: Silvestro
authors.initials: S
authors.lastname: Pettinato
authors.forename: Stefania
authors.initials: S
authors.lastname: Magliona
authors.forename: Renzo
authors.initials: R
authors.lastname: Ginanneschi
authors.forename: Luca
authors.initials: L
authors.lastname: Deiana
authors.forename: Angelo
authors.initials: A
authors.lastname: Zinellu
mesh_terms: D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D018744:DNA, Plant; D019075:Electrophoresis, Capillary / Q000379:methods*; D016678:Genome / Q000502:physiology*; D006868:Hydrolysis; D013696:Temperature
chemical_list: D018744:DNA, Plant; D004247:DNA