title: Inhibition of species of the Aspergillus section Nigri and ochratoxin a production in grapes by fusapyrone.
abstract: Fusapyrone (FP), an antifungal natural compound, was tested against the three main ochratoxigenic species of the Aspergillus section Nigri. The MICs at 24 h were 6.0, 11.6, and 9.9 mug/ml for Aspergillus carbonarius, Aspergillus tubingensis, and Aspergillus niger, respectively. Strong inhibition of growth and morphological changes were still observed at half the MIC after 7 days. The application of a 100 mug/ml FP solution in a laboratory assay on artificially inoculated grapes resulted in a significant reduction (up to 6 orders of magnitude) of A. carbonarius CFU counts. Dramatic reductions of the ochratoxin A (OTA) content, compared to the content of the positive control (average amount of OTA, 112.5 ng/g of grape; three experiments), were obtained with the application of either 100 or 50 mug/ml of FP (0.6 or 5.1 ng/g of grape, respectively).
authors.affiliation: Istituto di Scienze delle Produzioni Alimentari, CNR, Via Giovanni Amendola 122/O, 70125 Bari, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Favilla
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Pascale
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ricelli
authors.forename: Antonio
authors.initials: A
authors.lastname: Evidente
authors.forename: Carmine
authors.initials: C
authors.lastname: Amalfitano
authors.forename: Claudio
authors.initials: C
authors.lastname: Altomare
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009793:Ochratoxins / Q000096:biosynthesis*; D011753:Pyrones / Q000494:pharmacology*; D027843:Vitis / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D009793:Ochratoxins; D011753:Pyrones; C088397:fusapyrone