Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304.
A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 A resolution and a data set was collected at 100 K using Cu K alpha radiation from a rotating-anode X-ray source. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 49.27, c = 106.61 A. The calculated Matthews coefficient was 3.16 A(3) Da(-1), suggesting the presence of one molecule in the asymmetric unit.
Bahti
Pazilat
P
College of Life Science and Technology, Xinjiang University, Urumqi 830046, People's Republic of China.
Chen
Shunmei
S
Li
Yang
Y
Shaw
Neil
N
Zhang
Xuejun
X
Zhang
Min
M
Cheng
Chongyun
C
Song
Gaojie
G
Yin
Jie
J
Zhang
Hua
H
Che
Dongsheng
D
Abbas
Abdulla
A
Xu
Hao
H
Wang
Bi Cheng
BC
Liu
Zhi Jie
ZJ
D001105:Archaea / Q000737:chemistry*; D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D016366:Open Reading Frames
D019843:Archaeal Proteins