title: Specific oxidized phospholipids inhibit scavenger receptor bi-mediated selective uptake of cholesteryl esters.
abstract: We have recently demonstrated that specific oxidized phospholipids (oxPC(CD36)) accumulate at sites of oxidative stress in vivo such as within atherosclerotic lesions, hyperlipidemic plasma, and plasma with low high-density lipoprotein levels. oxPC(CD36) serve as high affinity ligands for the scavenger receptor CD36, mediate uptake of oxidized low density lipoprotein by macrophages, and promote a pro-thrombotic state via platelet scavenger receptor CD36. We now report that oxPC(CD36) represent ligands for another member of the scavenger receptor class B, type I (SR-BI). oxPC(CD36) prevent binding to SR-BI of its physiological ligand, high density lipoprotein, because of the close proximity of the binding sites for these two ligands on SR-BI. Furthermore, oxPC(CD36) interfere with SR-BI-mediated selective uptake of cholesteryl esters in hepatocytes. Thus, oxidative stress and accumulation of specific oxidized phospholipids in plasma may have an inhibitory effect on reverse cholesterol transport.
authors.affiliation: Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.
authors.forename: Mohammad Z
authors.initials: MZ
authors.lastname: Ashraf
authors.forename: Niladri S
authors.initials: NS
authors.lastname: Kar
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Choi
authors.forename: Robert G
authors.initials: RG
authors.lastname: Salomon
authors.forename: Maria
authors.initials: M
authors.lastname: Febbraio
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Podrez
mesh_terms: D001692:Biological Transport; D018955:CD36 Antigens / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008075:Lipoproteins, HDL / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008954:Models, Biological; D018384:Oxidative Stress; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051116:Receptors, Scavenger / Q000378:metabolism*; D051122:Scavenger Receptors, Class B / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D002788:Cholesterol Esters; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010743:Phospholipids; D051116:Receptors, Scavenger; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D002784:Cholesterol