title: Effects of cochlear ablation on amino acid concentrations in the chinchilla posteroventral cochlear nucleus, as compared to rat.
abstract: Using a microchemical approach, we measured changes of amino acid concentrations in the chinchilla caudal posteroventral cochlear nucleus (PVCN) after cochlear ablation to determine to what extent slow decreases of glutamate and aspartate concentrations after carboplatin treatment resulted from slower effects of cochlear damage in chinchillas than in rats and guinea pigs, as opposed to effects of carboplatin treatment being slower than those of cochlear ablation. Our results indicate that both factors are involved: decreases of glutamate and aspartate concentrations after cochlear ablation are much slower in chinchillas than in rats and guinea pigs, but they are much faster than the decreases after carboplatin treatment. Further, aspartate and glutamate concentrations in the chinchilla caudal PVCN decreased by larger amounts after cochlear ablation than in rats or guinea pigs, and there was a transient increase of aspartate concentration at short survival times. Detailed mapping of amino acid concentrations in the PVCN of a chinchilla with 1 month survival after cochlear ablation and a rat with 7 days' survival indicated that the reductions of glutamate and aspartate occurred throughout the PVCN but were somewhat larger in ventral and caudal parts in chinchilla. Any decreases in the adjacent granular region were very small. There were also sustained bilateral decreases in concentrations of other amino acids, notably GABA and glycine, in the caudal PVCN of cochlea-ablated chinchillas but not rats. The effects of cochlear ablation on the concentrations of most of these other amino acids in chinchilla caudal PVCN differed from those of carboplatin treatment. Thus, although a major effect of auditory nerve damage on the cochlear nucleus-decreases of glutamate and aspartate concentrations-occurs across species and types of lesions, the details of timing and magnitude and the effects on other amino acids can vary greatly.
authors.affiliation: Division of Otolaryngology and Dentistry, Department of Surgery, Mail Stop 1092, University of Toledo Health Science Campus, 3000 Arlington Avenue, Toledo, OH 43614, USA. donald.godfrey@utoledo.edu
authors.forename: D A
authors.initials: DA
authors.lastname: Godfrey
authors.forename: K
authors.initials: K
authors.lastname: Chen
authors.forename: M A
authors.initials: MA
authors.lastname: Godfrey
authors.forename: Y-M
authors.initials: YM
authors.lastname: Jin
authors.forename: K T
authors.initials: KT
authors.lastname: Robinson
authors.forename: C
authors.initials: C
authors.lastname: Hair
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects; D016190:Carboplatin / Q000494:pharmacology; D002682:Chinchilla / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003051:Cochlea / Q000293:injuries*; D017626:Cochlear Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004563:Electrochemistry; D007839:Functional Laterality; D008297:Male; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D013997:Time Factors
chemical_list: D000596:Amino Acids; D000970:Antineoplastic Agents; D018377:Neurotransmitter Agents; D016190:Carboplatin