title: Antinociceptive and anti-inflammatory effects of saponin and iridoid glycosides from Verbascum pterocalycinum var. mutense Hub.-Mor.
abstract: The anti-inflammatory and antinociceptive properties of four major compounds from the flowers of Verbascum pterocalycinum var. mutense were investigated. Saponin glycosides called ilwensisaponin A and C and iridoid glycosides known as ajugol and picroside IV were isolated from the methanolic extract. A dose-related anti-inflammatory and antinociceptive response were obtained in this study at doses of 100 and 200 mg/kg. The results of the evaluation of the anti-inflammatory activity induced by carrageenan and PGE1 showed that this species possesses active constituents that could diminish the cyclooxygenase activitiy. No effects were observed in the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model. Our results support the anti-inflammatory and analgesic effects of Verbascum pterocalycinum var. mutense. Ilwensisaponins A and C could explain in part the anti-inflammatory and analgesic activities of this species. Although antinociceptive and anti-inflammatory activities of ajugol and picroside IV were found insignificant in the statistical analysis, ilwensisaponin A and C showed notable activity without inducing any apparent acute toxicity as well as gastric damage.
authors.affiliation: Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, Etiler, 06330, Ankara, Turkey. esrak@gazi.edu.tr
authors.forename: Esra Küpeli
authors.initials: EK
authors.lastname: Akkol
authors.forename: I Irem
authors.initials: II
authors.lastname: Tatli
authors.forename: Zeliha S
authors.initials: ZS
authors.lastname: Akdemir
mesh_terms: D000700:Analgesics / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001241:Aspirin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D057889:Iridoid Glycosides; D008297:Male; D000432:Methanol; D051379:Mice; D008958:Models, Molecular; D009043:Motor Activity / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D032265:Verbascum / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D006027:Glycosides; D057889:Iridoid Glycosides; D010936:Plant Extracts; D028321:Plant Preparations; D011714:Pyrans; D012503:Saponins; C033250:harpagide; D001241:Aspirin; D000432:Methanol