[Determination of glutathione in a single human hepatocarcinoma cell using a microfluidic device coupled with electrochemical detection].
A method for the determination of glutathione (GSH) in a single human hepatocarcinoma cell, using a microfluidic chip coupled with electrochemical detection, was developed. In this method, the cell injection, loading, and cytolysis, as well as the transportation and detection of intracellular GSH were integrated in a microfluidic chip with a double-T injector and an end-channel amperometric detector. A single cell was loaded in the double-T injector on the microfluidic chip, using liquid pressure. The docked cell was perforated in the electrophoretic buffer containing digitonin and was lysed under a direct current electric field. The GSH from the single cell was electrokinetically transported to the separation channel and was electrochemically detected at an Au/Hg electrode. The GSH in the single cell was quantitatively determined using a calibration curve.
Wang
Wenlei
W
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China. wenleiwang@eyou.com
Jin
Wenrui
W
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D004563:Electrochemistry / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*
D005978:Glutathione