Inhibitory effects of Piper betle on production of allergic mediators by bone marrow-derived mast cells and lung epithelial cells.
The leaves of the Piper betle Linn. (Piperaceae) are used in traditional medicine and possess anti-oxidant, anti-bacterial, anti-fungal, anti-diabetic and radioprotective activities. However, little is known about their anti-allergic activity. Therefore, the effects of P. betle ethanolic extract (PE) on the production of histamine and granulocyte macrophage-colony-stimulating factor (GM-CSF) by murine bone marrow mast cells (BMMCs) and on the secretion of eotaxin and IL-8 by the human lung epithelial cell line, BEAS-2B, were investigated in vitro. PE significantly decreased histamine and GM-CSF produced by an IgE-mediated hypersensitive reaction, and inhibited eotaxin and IL-8 secretion in a TNF-alpha and IL-4-induced allergic reaction. The results suggest that P. betle may offer a new therapeutic approach for the control of allergic diseases through inhibition of production of allergic mediators.
Wirotesangthong
Mali
M
Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. mali.w@chula.ac.th <mali.w@chula.ac.th>
Inagaki
Naoki
N
Tanaka
Hiroyuki
H
Thanakijcharoenpath
Witchuda
W
Nagai
Hiroichi
H
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D054413:Chemokine CCL11 / Q000096:biosynthesis; D054423:Chemokine CCL24 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D031721:Piper betle; D010936:Plant Extracts / Q000494:pharmacology*
D018926:Anti-Allergic Agents; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D016207:Cytokines; D016209:Interleukin-8; D010936:Plant Extracts; D016178:Granulocyte-Macrophage Colony-Stimulating Factor