title: Next generation adoptive immunotherapy--human T cells as carriers of therapeutic nanoparticles.
abstract: An important step in adoptive immunotherapy in general and specifically with respect to cancer treatment is the initiation of an inflammatory T cell response at the tumor site. Here we suggest a new concept for a controlled inflammatory response in which the intrinsic cytotoxic properties of T cells are upgraded with the properties of nanoparticles transfected into the T cells during the ex vivo expansion process. We report in vitro upgrading of human T cells using PEGylated boron carbide nanoparticles functionalised with a translocation peptide aimed at Boron Neutron Capture Therapy (BNCT). A key finding is that the metabolism of such upgraded human T cells were not affected by a payload of 0.13 pg boron per cell and that the nanoparticles were retained in the cell population after several cell divisions. This is vital for transporting nanoparticles by T cells to the tumor site.
authors.affiliation: Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark.
authors.forename: M W
authors.initials: MW
authors.lastname: Mortensen
authors.forename: L
authors.initials: L
authors.lastname: Kahns
authors.forename: T
authors.initials: T
authors.lastname: Hansen
authors.forename: P G
authors.initials: PG
authors.lastname: Sorensen
authors.forename: O
authors.initials: O
authors.lastname: Björkdahl
authors.forename: M R
authors.initials: MR
authors.lastname: Jensen
authors.forename: H J G
authors.initials: HJ
authors.lastname: Gundersen
authors.forename: T
authors.initials: T
authors.lastname: Bjørnholm
mesh_terms: D000595:Amino Acid Sequence; D004337:Drug Carriers; D005434:Flow Cytometry; D006801:Humans; D016219:Immunotherapy, Adoptive; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000627:therapeutic use*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D004337:Drug Carriers