title: [The pilot study of the effect of paclitaxel by local slow delivery on intimal hyperplasia of vascular anastomosis after vascular graft bypass].
abstract: OBJECTIVE
  To develop a new way to prevent restenosis in the anastomotic site due to intimal hyperplasia after vascular graft bypass (VGB) in peripheral arteries.
  
  
  METHODS
  Five mongrel dogs received bilateral iliac-femoral VGB and their arteries between the graft were ligated and cut off under general anaesthesia. The mixture of the paclitaxel and fibrin gel (FG) were randomly sprayed onto one side of grafts including distal and proximal anastomotic site, and the fibrin gel served as control were sprayed onto the other one. The bilateral grafts including distal and proximal anastomotic site were harvested four weeks postoperationally and the anastomotic sites were observed grossly, pathologically and by electron microscopy. The intimal thickness and area of each anastomotic site were measured, then the data were analysed statistically.
  
  
  RESULTS
  The bilateral grafts of all dogs were patent and the neointima of all anastomotic sites have been seen grossly. The neointimal thickness and area of the experimental side were significantly reduced compared with the control side (P < 0.05). Scanning electron microscopy showed that the anastomotic intima of the experimental side was covered with one layer of intact and regular endothelium cells with deposition of little blood components, but the anastomotic intima of the control side was covered with irregular endothelium cells and deposited with a lot of blood cells and fibrins. Transmission electron microscopy showed the anastomotic intima of the control side that rich in vascular smooth muscle cells and the matrix of the intima was composed of regular collagenous fibers, and that of the experimental side consisted of several types of cells with a lot of foreign particles in the matrix.
  
  
  CONCLUSIONS
  It is safe and effective to locally use low dose of paclitaxel carried by FG in the prevention of vascular anastomotic site intimal hyperplasia. Paclitaxel molecules can penetrate the graft wall and stay in the anastomotic intima more than four weeks postoperationally.
authors.affiliation: Department of Vascular Surgery, Peking University People's Hospital, Beijing 100044, China. yl710908@yahoo.com.cn
authors.forename: Lian
authors.initials: L
authors.lastname: Yuan
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zhang
authors.forename: Yan-Kui
authors.initials: YK
authors.lastname: Li
authors.forename: Qing-le
authors.initials: QL
authors.lastname: Li
authors.forename: Ke-Qiang
authors.initials: KQ
authors.lastname: Zhao
authors.forename: Kun-Kun
authors.initials: KK
authors.lastname: Sun
authors.forename: Da-He
authors.initials: DH
authors.lastname: Xie
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D004285:Dogs; D006965:Hyperplasia; D017239:Paclitaxel / Q000494:pharmacology*; D010865:Pilot Projects; D011184:Postoperative Period; D054047:Surgical Stomas / Q000473:pathology*; D017539:Tunica Intima / Q000187:drug effects* / Q000473:pathology / Q000601:surgery
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel