Enzymatic permeabilization of the thecate dinoflagellate Alexandrium minutum (Dinophyceae) yields detection of intracellularly associated bacteria via catalyzed reporter deposition-fluorescence in situ hybridization.
The enzymatic permeabilization procedure described here allows the detection of intracellular bacteria in the thecate dinoflagellate Alexandrium minutum by using catalyzed reporter deposition-fluorescence in situ hybridization. The combined use of propidium iodide and calcofluor for confocal laser scanning microscopy, together with general and specific fluorescent bacterial probes, demonstrated the intracellular presence of bacteria, including members of the phylum Bacteroidetes.
Palacios
Lucía
L
Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Cantoblanco, Madrid, Spain. imarin@cbm.uam.es
Marín
Irma
I
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D004141:Dinoflagellida / Q000737:chemistry / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D018613:Microscopy, Confocal