title: Hydroxyl radical generation and oxidative stress in Carassius auratus liver, exposed to pyrene.
abstract: This paper studied the hydroxy radical generation and oxidative stress in the liver of goldfish Carassius auratus under the effect of pyrene. Fish were exposed to different concentrations (0.001, 0.005, 0.01, 0.05 and 0.1 mg/L) of pyrene for 10 days, with one group assigned as control. Based on the hyperfine splitting constants and shape of the electron paramagnetic resonance (EPR) spectrum, the free radical which was generated in fish liver was identified as hydroxyl radical ((*)OH). The (*)OH signal intensity showed a significant increase compared with the control. The changes of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST) were detected. The reduced glutathione (GSH) level decreased significantly while oxidized glutathione (GSSG) level was increased at higher concentration (0.005-0.1 mg/L), resulting in a decreased GSH/GSSG ratio, and the malondialdehyde (MDA) content increased significantly at 0.005-0.1 mg/L pyrene. The results clearly showed that C. auratus was subjected to oxidative stress and damage when exposed to pyrene.
authors.affiliation: Department of Hydrosciences, Nanjing University, Nanjing 210093, China. sunyy1999@163.com
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Yin
authors.forename: Jingfei
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Wang
authors.forename: Jichun
authors.initials: J
authors.lastname: Wu
authors.forename: Yuqun
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D011721:Pyrenes / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D011721:Pyrenes; D014874:Water Pollutants, Chemical; D017665:Hydroxyl Radical; C030984:pyrene; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione; D019803:Glutathione Disulfide