Micrococcal nuclease analysis of chromatin structure.
This unit describes methodology for using micrococcal nuclease to investigate the presence of nucleosomes at a particular location in chromatin and to map the positions of nucleosomes at various levels of resolution. The approaches are readily adaptable to other probes of chromatin structure that cause DNA cleavage. Results obtained from such chromatin studies provide a structural view of the molecular environment of gene in their native context in cells.
Zaret
Ken
K
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D053837:DNA Cleavage; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009707:Nucleosomes / Q000737:chemistry
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease