Preparation of myeloma cells.
In this unit, myeloma cells are cultured to ensure their sensitivity to the HAT selection medium used after cell fusion. Cell culture conditions are adjusted such that the Sp2/0 cells are in the log phase of growth and exhibit high viability at the time of collection for fusion. A support protocol is provided to determine the number of viable cells present in the cell culture.
Fuller
S A
SA
Allelix Inc., Mississauga, Ontario.
Takahashi
M
M
Hurrell
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification*; D018929:Cell Culture Techniques; D042381:Granulocyte Precursor Cells / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal