title: Rapid and sensitive on-line liquid chromatographic/tandem mass spectrometric determination of an ethylene oxide-DNA adduct, N7-(2-hydroxyethyl)guanine, in urine of nonsmokers.
abstract: Ethylene oxide (EtO) is classified as a known human carcinogen. The formation of EtO-DNA adducts is considered as an important early event in the EtO carcinogenic process. An isotope-dilution on-line solid-phase extraction and liquid chromatography coupled with tandem mass spectrometry method was then developed to analyze one of the EtO-DNA adducts, N7-(2-hydroxyethyl)guanine (N7-HEG), in urine of 46 nonsmokers with excellent accuracy, sensitivity and specificity. The merits of this method include small sample volume (only 120 microL urine required), automated sample cleanup, and short total run time (12 minutes per sample). This method demonstrates its high-throughput capacity for future molecular epidemiology studies on the potential health effects resulting from the low-dose EtO exposure.
authors.affiliation: Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 35 Keyan Rd., Zhunan Town, Miaoli County 35053, Taiwan.
authors.forename: Chih-Chun Jean
authors.initials: CC
authors.lastname: Huang
authors.forename: Wei-Chung
authors.initials: WC
authors.lastname: Shih
authors.forename: Chia-Fang
authors.initials: CF
authors.lastname: Wu
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Chen
authors.forename: Yi-Lung
authors.initials: YL
authors.lastname: Chen
authors.forename: Ying-Huei
authors.initials: YH
authors.lastname: Lin
authors.forename: Kuen-Yuh
authors.initials: KY
authors.lastname: Wu
mesh_terms: D000393:Air Pollutants / Q000737:chemistry*; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000652:urine*; D005027:Ethylene Oxide / Q000737:chemistry*; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D012016:Reference Values; D012907:Smoking / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D018736:DNA Adducts; C035018:N(7)-hydroxyethylguanine; D006147:Guanine; D004247:DNA; D005027:Ethylene Oxide