title: Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.).
abstract: Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 microM 2,4-D and 1 microM Kinetin or 5 microM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 microM 2,4,5-T or the combination of 10 microM 2,4-D and 1 microM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry.
authors.affiliation: Laboratoire de physiologie végétale, département de biologie, faculté des sciences, BP 1014, Rabat, Maroc.
authors.forename: Zine
authors.initials: Z
authors.lastname: El Abidine Triqui
authors.forename: Abdelkarim
authors.initials: A
authors.lastname: Guédira
authors.forename: Averil
authors.initials: A
authors.lastname: Chlyah
authors.forename: Hassane
authors.initials: H
authors.lastname: Chlyah
authors.forename: Vongthip
authors.initials: V
authors.lastname: Souvannavong
authors.forename: Robert
authors.initials: R
authors.lastname: Haïcour
authors.forename: Darasinh
authors.initials: D
authors.lastname: Sihachakr
mesh_terms: D000362:Agar / Q000494:pharmacology*; D003470:Culture Media; D047108:Embryonic Development; D005838:Genotype; D007210:Indoleacetic Acids / Q000494:pharmacology*; D027723:Ipomoea batatas / Q000196:embryology* / Q000235:genetics*; D011003:Ploidies; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D007210:Indoleacetic Acids; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D000362:Agar