Effects of hyperbaric oxygen on energy production and xanthine oxidase levels in striated muscle tissue of healthy rats.
We investigated the effects of hyperbaric oxygen (HBO) treatment on striated muscle tissue in healthy rats. The treatment group of rats (n=16) was given HBO daily on weekdays for 2 h over a 4-week period while a control group (n=8) was not treated. Tissue samples were taken from the left and right vastus lateralis before and after the HBO treatment period, respectively, for all rats in both groups. Levels of adenosine monophosphate (AMP), adenosine diphosphate, andenosine triphosphate (ATP) and xanthine oxidase in the muscle tissue were determined. HBO treatment caused a statistically significant increase in ATP (p=0.001) and decrease in AMP (p=0.02) in the HBO-treated group, while there were no significant differences in metabolites in the control group. These results suggest that HBO treatment induces an increase in the ATP levels of muscle tissue with normal mitochondria. Thus, HBO might have some beneficial effects in the treatment of heteroplasmic mitochondrial disease, where normal and defective mitochondria coexist.
Kurt
Bülent
B
Department of Pathology, Gulhane Military Medical Academy and Medical School, Etlik, Ankara, Turkey. bkurt_md@yahoo.com
Kurt
Yasemin
Y
Karslioğlu
Yildirim
Y
Topal
Turgut
T
Erdamar
Hüsamettin
H
Korkmaz
Ahmet
A
Türközkan
Nurten
N
Yaman
Halil
H
Odabaşi
Zeki
Z
Günhan
Omer
O
D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D006931:Hyperbaric Oxygenation; D066298:In Vitro Techniques; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors; D014969:Xanthine Oxidase / Q000378:metabolism*
D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D014969:Xanthine Oxidase; D013385:Succinate Dehydrogenase