A single oral sensitization to peanut without adjuvant leads to anaphylaxis in mice.
BACKGROUND
A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response.


METHODS
C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis.


RESULTS
Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels.


CONCLUSIONS
Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.
Proust
Barbara
B
EA 3999 Allergic Diseases: Diagnosis and Therapeutics, Department of Internal Medicine, Clinical Immunology and Allergology, University Hospital, Central Hospital, Nancy, France.
Astier
Catherine
C
Jacquenet
Sandrine
S
Ogier
Virginie
V
Magueur
Erwan
E
Roitel
Olivier
O
Belcourt
Christelle
C
Morisset
Martine
M
Moneret-Vautrin
Denise A
DA
Bihain
Bernard E
BE
Kanny
Gisèle
G
D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D001831:Body Temperature / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D021183:Peanut Hypersensitivity / Q000276:immunology*; D012119:Respiration / Q000276:immunology; D012882:Skin Tests; D013047:Specific Pathogen-Free Organisms
D007073:Immunoglobulin E