Identifying chromosomal targets of DNA-binding proteins by Sequence Tag Analysis of Genomic Enrichment (STAGE).
Sequence Tag Analysis of Genomic Enrichment (STAGE) is a method for experimentally identifying the in vivo chromosomal targets of DNA-binding proteins in any sequenced genome. STAGE generates 21-bp tags derived from DNA isolated by chromatin immunoprecipitation (ChIP; UNIT 21.3). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA-binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA-binding protein in vivo. STAGE can be applied to any sequenced genome to identify targets of DNA-binding proteins without requiring extensive microarray resources.
Kim
Jonghwan
J
University of Texas at Austin, Austin, Texas, USA.
Iyer
Vishwanath R
VR
D035843:Biomedical Research / Q000379:methods*; D047369:Chromatin Immunoprecipitation; D002874:Chromosome Mapping / Q000379:methods*; D002875:Chromosomes / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D020224:Expressed Sequence Tags; D016678:Genome; D007202:Indicators and Reagents; D016324:Sequence Tagged Sites
D004268:DNA-Binding Proteins; D007202:Indicators and Reagents