Rhizopus delemar is the proper name for Rhizopus oryzae fumaric-malic acid producers.
The zygomycete Rhizopus oryzae currently is identified by sporangiophore morphology and growth temperature, but heterogeneity of the species has been reported. We examined the suitability of organic acid production as an effective taxonomic character for reclassification of the species. Strains were divided into two groups, LA (lactic acid producer) and FMA (fumaric-malic acid producers) according to organic acid production. These grouping were confirmed as phylogenetically distinct because analyses of rDNA ITS, lactate dehydrogenase B, actin, translation elongation factor-1alpha and genomewide AFLP resolved the same two exclusive clusters, corresponding with the organic acid grouping. Reclassification of strains in the FMA group as R. delemar was proposed.
Abe
Ayumi
A
Laboratory of Applied Microbiology, Research Faculty of Agriculture, Hokkaido University, Kita-9 Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
Oda
Yuji
Y
Asano
Kozo
K
Sone
Teruo
T
D000199:Actins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005650:Fumarates / Q000378:metabolism*; D045022:Lactate Dehydrogenases / Q000737:chemistry / Q000235:genetics; D019344:Lactic Acid / Q000378:metabolism*; D008293:Malates / Q000378:metabolism*; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012233:Rhizopus / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
D000199:Actins; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005650:Fumarates; D008293:Malates; D020648:Peptide Elongation Factor 1; D019344:Lactic Acid; C030298:malic acid; C032005:fumaric acid; D045022:Lactate Dehydrogenases