Structural interaction between DsrE-DsrF-DsrH proteins involved in the transport of electrons in the dsr operon.
Biological redox reactions of inorganic sulfur compounds are important for the proper maintenance of environmental sulfur balance. These reactions are mediated by phylogeneticaly diverse set of microorganisms. The protein complex that is involved in such redox reactions of sulfur compounds is the complex encoded by dsr operon. The ecological and industrial importance of these microorganisms led us to investigate the structural details of the mechanism of the process of electron transport during such redox reactions performed by the dsr operon. Among the gene products of the operon, the proteins DsrE, DsrF, and DsrH are small soluble cytoplasmic proteins acting as alpha2beta2gamma2 heterohexamer and are involved in the process of electron transport in these ecologically as well as industrially important microorganisms. Since no structural details of the proteins were available we employed homology modeling to construct the three-dimensional structures of the DsrE, DsrF, and DsrH from Chlorobium tepidum. The putative three dimensional structures of the proteins were predicted from the models. Since DsrE, DsrF, and DsrH proteins act as a hetero-hexameric complex, the modeled proteins were subjected to molecular docking analyses to generate the model of the biochemically active complex. This allowed us to predict the probable binding modes of the proteins as well as the biochemical and the structural basis of the mechanism of the electron transport process by this complex. The hexamerization of the proteins would help to bring the Cys residues in close proximity, which enables the complex to actively take part electron transport process.
Bagchi
Angshuman
A
Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, Nadia, West Bengal, India. angshuman_bagchi@yahoo.com
Ghosh
Tapash Chandra
TC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D041883:Chlorobium / Q000235:genetics / Q000378:metabolism; D004579:Electron Transport / Q000502:physiology*; D004583:Electrons; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009876:Operon; D010084:Oxidation-Reduction; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D001426:Bacterial Proteins