title: Expression of chemokine receptor CX3CR1 in infants with respiratory syncytial virus bronchiolitis.
abstract: Respiratory syncytial virus (RSV) glycoprotein G mimics fractalkine, a CX(3)C chemokine, which mediates chemotaxis of leukocytes expressing its receptor, CX(3)CR1. The aim of this study was to examine the relationship between RSV infection and expression of perforin and IFN-gamma in CX(3)CR1-expressing peripheral blood CD8(+) T cells. Samples were collected from infants with RSV bronchiolitis, both in the acute and convalescence phase (n = 12), and from their age- and sex-matched healthy controls (n = 15). Perforin expression and IFN-gamma secretion in CX(3)CR1(+) CD8(+) T cells were assessed by four-color flow cytometry. The NF-kappaB p50 and p65 subunit levels were also determined as markers of RSV-induced inflammation. Study results showed perforin and CX(3)CR1 expression to be significantly lower in the convalescent phase of infected infants than in healthy controls. There was no significant difference in IFN-gamma secretion and NF-kappaB binding activity between two time-points in RSV-infected infants, or when compared with healthy controls. Infants with prolonged wheezing had lower acute-phase CX(3)CR1 levels in peripheral blood. These data indicate existence of an event persisting after acute RSV infection that is able to modulate effector functions of cytotoxic T cells, and also link disease severity with CX(3)CR1 expression.
authors.affiliation: Institute of Immunology, Zagreb University Hospital Center, Zagreb, Croatia.
authors.forename: Alma-Martina
authors.initials: AM
authors.lastname: Cepika
authors.forename: Alenka
authors.initials: A
authors.lastname: Gagro
authors.forename: Ana
authors.initials: A
authors.lastname: Bace
authors.forename: Dorian
authors.initials: D
authors.lastname: Tjesic-Drinkovic
authors.forename: Jadranka
authors.initials: J
authors.lastname: Kelecic
authors.forename: Tamara
authors.initials: T
authors.lastname: Baricic-Voskresensky
authors.forename: Mladen
authors.initials: M
authors.lastname: Matic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Drazenovic
authors.forename: Igor
authors.initials: I
authors.lastname: Marinic
authors.forename: Gordana
authors.initials: G
authors.lastname: Mlinaric-Galinovic
authors.forename: Duska
authors.initials: D
authors.lastname: Tjesic-Drinkovic
authors.forename: Zvonimir
authors.initials: Z
authors.lastname: Vrtar
authors.forename: Sabina
authors.initials: S
authors.lastname: Rabatic
mesh_terms: D000208:Acute Disease; D015415:Biomarkers / Q000378:metabolism; D001990:Bronchiolitis, Viral / Q000097:blood / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D002467:Cell Nucleus / Q000378:metabolism; D003289:Convalescence; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D054353:Perforin / Q000096:biosynthesis; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D018357:Respiratory Syncytial Virus Infections / Q000097:blood / Q000276:immunology*
chemical_list: D015415:Biomarkers; D000073883:CX3C Chemokine Receptor 1; C095235:CX3CR1 protein, human; D019707:Receptors, Chemokine; D054353:Perforin; D007371:Interferon-gamma