title: Influence of human dentine on the antibacterial activity of self-etching adhesive systems against cariogenic bacteria.
abstract: OBJECTIVE
  The incorporation of antibacterial agents into adhesive systems has been proposed to eliminate residual bacteria from dentine. This study used the agar diffusion method to evaluate the antibacterial activity of Clearfil Protect Bond (CPB), Clearfil SE Bond (CSEB), Clearfil Tri-S Bond (C3SB) and Xeno-III (XIII) self-etching adhesive systems, with or without light-activation, against cariogenic bacteria, and to assess the influence of human dentine on the antibacterial activity of these materials.
  
  
  METHODS
  An aliquot of 10 microl per material (and individual components) were pipetted onto paper and dentine discs distributed in Petri dishes containing bacterial culture in BHI agar. Positive control was 0.2% chlorhexidine digluconate (CHX).
  
  
  RESULTS
  After incubation, the adhesive components of CPB and CSEB, liquid A of XIII and C3SB did not present antibacterial activity when applied to paper discs. The non-light-activated CPB primer+adhesive promoted the greatest inhibition of Streptococcus mutans (p<0.05), whereas with light-activation, there was no significant difference between primer+adhesive and primer alone. For Lactobacillus acidophilus, CPB primer presented the greatest antibacterial activity in both light-activation conditions (p<0.05). Regarding the dentine discs, only CHX promoted an inhibitory effect, though less intense than on paper discs (p<0.05). CHX presented greater antibacterial activity against S. mutans than against L. acidophilus (p<0.05).
  
  
  CONCLUSIONS
  Light-activation significantly reduced the antibacterial activity of the self-etching adhesive systems; MDPB incorporation contributed to the effect of adhesive systems against cariogenic bacteria; the components eluted from the adhesive systems were not capable to diffuse through 400 microm-thick dentine disc to exert their antibacterial activity against cariogenic bacteria.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Araraquara, São Paulo State University, UNESP, Rua Humaitá, 1680 Araraquara, SP, Brazil.
authors.forename: Juliana O
authors.initials: JO
authors.lastname: Gondim
authors.forename: Cristiane
authors.initials: C
authors.lastname: Duque
authors.forename: Josimeri
authors.initials: J
authors.lastname: Hebling
authors.forename: Elisa M A
authors.initials: EM
authors.lastname: Giro
mesh_terms: D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D003804:Dentin / Q000382:microbiology; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D005779:Immunodiffusion; D007779:Lactobacillus acidophilus / Q000187:drug effects; D008027:Light; D011726:Pyridinium Compounds / Q000494:pharmacology*; D019279:Resin Cements / Q000737:chemistry / Q000494:pharmacology*; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D017220:Dentin-Bonding Agents; D011726:Pyridinium Compounds; D019279:Resin Cements; C089392:methacryloyloxydodecylpyridinium bromide