Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis.
Cloning vectors derived from filamentous phage are extremely useful because they allow cloned DNA to be isolated as either single- or double-stranded DNA. This unit contains protocols for preparing both forms of DNA and for characterizing inserts in M13-derived vectors. A protocol is also presented for preparing single-stranded DNA from plasmids using superinfection with helper phage. This method is advantageous because it allows cloned DNA to be maintained in the form of a plasmid while permitting single-stranded DNA to be isolated for DNA sequencing.
Ellington
A
A
University of Texas, Austin, Texas, USA.
Pollard
J D
JD
D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000302:isolation & purification*
D009841:Oligonucleotides