Isolation of proteins for microsequence analysis.
This unit presents a method for determining the amino-terminal sequence of polypeptides or proteins. It is particularly appropriate for large fragments of insoluble or hydrophobic proteins or proteins that cannot be purified to >90% molar purity without electrophoresis. If the protein is blocked at the amino terminus, chemical cleavage or partial enzymatic digestion must be performed prior to electrophoresis. Upon isolation, the internal amino acid sequence is analyzed as described.
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Center for Biologics Evaluation & Research, Food and Drug Administration, Bethesda, Maryland, USA.
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D020539:Sequence Analysis, Protein / Q000379:methods*; D005465:Fluorocarbon Polymers
D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers