title: A single oral sensitization to peanut without adjuvant leads to anaphylaxis in mice.
abstract: BACKGROUND
  A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response.
  
  
  METHODS
  C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis.
  
  
  RESULTS
  Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels.
  
  
  CONCLUSIONS
  Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.
authors.affiliation: EA 3999 Allergic Diseases: Diagnosis and Therapeutics, Department of Internal Medicine, Clinical Immunology and Allergology, University Hospital, Central Hospital, Nancy, France.
authors.forename: Barbara
authors.initials: B
authors.lastname: Proust
authors.forename: Catherine
authors.initials: C
authors.lastname: Astier
authors.forename: Sandrine
authors.initials: S
authors.lastname: Jacquenet
authors.forename: Virginie
authors.initials: V
authors.lastname: Ogier
authors.forename: Erwan
authors.initials: E
authors.lastname: Magueur
authors.forename: Olivier
authors.initials: O
authors.lastname: Roitel
authors.forename: Christelle
authors.initials: C
authors.lastname: Belcourt
authors.forename: Martine
authors.initials: M
authors.lastname: Morisset
authors.forename: Denise A
authors.initials: DA
authors.lastname: Moneret-Vautrin
authors.forename: Bernard E
authors.initials: BE
authors.lastname: Bihain
authors.forename: Gisèle
authors.initials: G
authors.lastname: Kanny
mesh_terms: D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D001831:Body Temperature / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D021183:Peanut Hypersensitivity / Q000276:immunology*; D012119:Respiration / Q000276:immunology; D012882:Skin Tests; D013047:Specific Pathogen-Free Organisms
chemical_list: D007073:Immunoglobulin E