Analysis of toll-like receptor-dependent production of proinflammatory cytokines in vitro by human peripheral blood mononuclears of donors and patients with primary immunodeficiency.
The production of TNF-alpha and IFN-alpha cytokines by peripheral blood mononuclears in response to stimulation by TLR2/6, TLR4, TLR5, TLR9 ligands (zymosan, LPS, flagellin, and CpG-oligodeoxynucleotide, respectively) was studied in donors and patients with common variable immunodeficiency. Individual characteristics of TNF-alpha production by mononuclears were revealed in donors. Reduced stimulated production of TNF-alpha in response to stimulation with TLR4 and TLR5 ligands in vitro was detected in patients with common variable immunodeficiency.
Koval'chuk
L V
LV
Russian State Medical University, Moscow. immun@rsmu.ru
Khoreva
M V
MV
Varivoda
A S
AS
Pashchenko
O E
OE
Gracheva
L A
LA
Bykova
L P
LP
Kondratenko
I V
IV
Bologov
A A
AA
D000293:Adolescent; D000328:Adult; D001782:Blood Donors; D002648:Child; D017074:Common Variable Immunodeficiency / Q000097:blood / Q000503:physiopathology*; D005260:Female; D005408:Flagellin / Q000494:pharmacology; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D051193:Toll-Like Receptors / Q000187:drug effects / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D015054:Zymosan / Q000494:pharmacology
C408982:CPG-oligonucleotide; D016898:Interferon-alpha; D008070:Lipopolysaccharides; D009838:Oligodeoxyribonucleotides; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D005408:Flagellin; D015054:Zymosan; D011070:Poly I-C