Immunoscreening of fusion proteins produced in lambda plaques.
Screening large numbers of plaques containing particular proteins is accomplished by techniques that are analogous to those described for screening with radioactive DNA probes. However, in the basic protocol described here, the plaques are screened with antibodies specific to the desired proteins. An alternate protocol provides a method for increasing the amount of recombinant protein in each plaque by inducing expression from the lac promoter that directs its expression.
St John
T P
TP
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
D000906:Antibodies; D010582:Bacteriophage lambda / Q000235:genetics* / Q000254:growth & development; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D015723:Gene Library; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins / Q000032:analysis* / Q000276:immunology; D010948:Viral Plaque Assay / Q000379:methods
D000906:Antibodies; D015342:DNA Probes; D004279:DNA, Viral; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins