title: A PLA/calcium phosphate degradable composite material for bone tissue engineering: an in vitro study.
abstract: Biodegradable polymers reinforced with an inorganic phase such as calcium phosphate glasses may be a promising approach to fulfil the challenging requirements presented by 3D porous scaffolds for tissue engineering. Scaffolds' success depends mainly on their biological behaviour. This work is aimed to the in vitro study of polylactic acid (PLA)/CaP glass 3D porous constructs for bone regeneration. The scaffolds were elaborated using two different techniques, namely solvent-casting and phase-separation. The effect of scaffolds' micro and macrostructure on the biological response of these scaffolds was assayed. Cell proliferation, differentiation and morphology within the scaffolds were studied. Furthermore, polymer/glass scaffolds were seeded under dynamic conditions in a custom-made perfusion bioreactor. Results indicate that the final architecture of the solvent-cast or phase separated scaffolds have a significant effect on cells' behaviour. Solvent-cast scaffolds seem to be the best candidates for bone tissue engineering. Besides, dynamic seeding yielded a higher seeding efficiency in comparison with the static method.
authors.affiliation: Department of Materials Science, Technical University of Catalonia, Barcelona, Spain.
authors.forename: Montse
authors.initials: M
authors.lastname: Charles-Harris
authors.forename: Martin A
authors.initials: MA
authors.lastname: Koch
authors.forename: Melba
authors.initials: M
authors.lastname: Navarro
authors.forename: Damien
authors.initials: D
authors.lastname: Lacroix
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Engel
authors.forename: Josep A
authors.initials: JA
authors.lastname: Planell
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000378:metabolism*; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism; D010539:Permeability; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012997:Solvents / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; D012997:Solvents; D019344:Lactic Acid; C033616:poly(lactide); C020243:calcium phosphate