title: Plasma and gastric mucosal 5-hydroxytryptamine concentrations following cold water intake in patients with diarrhea-predominant irritable bowel syndrome.
abstract: OBJECTIVE
  The purpose of the present paper was to investigate the effects of cold water intake on 5-hydroxytryptamine (5-HT) and its metabolite 5-hydroxyindole acetic acid (5-HIAA) in diarrhea-predominant irritable bowel syndrome (d-IBS) patients, and to observe the relationship between 5-HT and symptomatology.
  
  
  METHODS
  The plasma 5-HT/5-HIAA concentrations at 0, 30 min, 60 min, 90 min, 120 min, 150 min and 180 min following cold or warm water intake were investigated in 32 female subjects with d-IBS and 21 healthy female subjects. Gastric mucosal 5-HT under fasting conditions and following water intake were further investigated in 15 d-IBS patients and nine healthy subjects. Symptomatology was assessed throughout the study.
  
  
  RESULTS
  The plasma 5-HT concentrations in IBS patients were significantly higher than those of controls at 30 min (P = 0.022), 60 min (P < 0.001), 90 min (P < 0.001), 120 min (P < 0.001) and 150 min (P = 0.001) after cold water intake. The peak plasma 5-HT/5-HIAA and area under the curve for 5-HT/5-HIAA were also higher in d-IBS patients (P < 0.001). Gastric mucosal 5-HT in d-IBS patients and controls did not show any significant differences both under fasting condition (P = 0.596) and after cold water intake (P = 0.426). Last, the d-IBS patients with symptoms had higher 5-HT concentration (P < 0.001) and there was a positive correlation (r = 0.714, P = 0.001)between the symptomatology and plasma 5-HT level.
  
  
  CONCLUSIONS
  These data suggest that symptomatology following cold water intake may be associated with increased plasma 5-HT concentrations in female subjects with d-IBS.
authors.affiliation: Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: Xiu Li
authors.initials: XL
authors.lastname: Zuo
authors.forename: Yan Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Xiao Zhong
authors.initials: XZ
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.forename: Yu Ting
authors.initials: YT
authors.lastname: Guo
authors.forename: Xue Feng
authors.initials: XF
authors.lastname: Lu
authors.forename: Jun Man
authors.initials: JM
authors.lastname: Li
authors.forename: Paul V
authors.initials: PV
authors.lastname: Desmond
mesh_terms: D000328:Adult; D019540:Area Under Curve; D001792:Blood Platelets; D003080:Cold Temperature; D003967:Diarrhea / Q000097:blood* / Q000150:complications; D004326:Drinking; D005215:Fasting; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D043183:Irritable Bowel Syndrome / Q000097:blood* / Q000150:complications*; D008875:Middle Aged; D012701:Serotonin / Q000097:blood*
chemical_list: D012701:Serotonin; D006897:Hydroxyindoleacetic Acid