Glucose-6-phosphate dehydrogenase deficiency enhances human coronavirus 229E infection.
The host cellular environment is a key determinant of pathogen infectivity. Viral gene expression and viral particle production of glucose-6-phosphate dehydrogenase (G6PD)-deficient and G6PD-knockdown cells were much higher than their counterparts when human coronavirus (HCoV) 229E was applied at 0.1 multiplicity of infection. These phenomena were correlated with increased oxidant production. Accordingly, ectopic expression of G6PD in G6PD-deficient cells or addition of antioxidant (such as alpha-lipoic acid) to G6PD-knockdown cells attenuated the increased susceptibility to HCoV 229E infection. All experimental data indicated that oxidative stress in host cells is an important factor in HCoV 229E infectivity.
Wu
Yi-Hsuan
YH
Graduate Institute of Basic Medical Sciences, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan.
Tseng
Ching-Ping
CP
Cheng
Mei-Ling
ML
Ho
Hung-Yao
HY
Shih
Shin-Ru
SR
Chiu
Daniel Tsun-Yee
DT
D002478:Cells, Cultured; D003139:Common Cold / Q000201:enzymology / Q000821:virology*; D028941:Coronavirus 229E, Human / Q000254:growth & development* / Q000378:metabolism; D018352:Coronavirus Infections / Q000201:enzymology* / Q000821:virology*; D005347:Fibroblasts / Q000201:enzymology / Q000821:virology; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000378:metabolism / Q000821:virology*; D006801:Humans; D018384:Oxidative Stress; D008063:Thioctic Acid / Q000378:metabolism; D014771:Virion / Q000378:metabolism
D008063:Thioctic Acid; D005954:Glucosephosphate Dehydrogenase