[Study of the effect of hepatitis C virus core protein on interferon-induced antiviral genes expression and its mechanisms].
To study the effect of HCV core protein on the interferon-induced antiviral genes expression and its mechanisms. Methods HepG2 cells were transiently transfected with HCV core protein expression plasmid and the blank plasmid respectively. RT-PCR was used to analyze the effect of HCV core protein on PKR and 2'-5'OAS expression. The effect of HCV core protein on ISRE-medicated gene expression was detected by luciferase activity assay. Western-blot assay was performed to observe the change of mRNA and protein levels of SOCS3, STAT1 and p-STAT1 following HCV core expression. In the presence of HCV core protein, the transcription of PKR and 2'-5' OAS are down-regulated. ISRE-medicated reporter gene expression and STAT1 phosphorylation were inhibited. The transcription and expression of SOCS3 were induced compared with blank plasmid-transfected group. In HepG2 cells, HCV core protein can down-regulate the expression of some interferon-induced antiviral genes, which involves the induction of SOCS3 and the inhibition of STAT1 phosphorylation.
Chang
Yan-Zi
YZ
Department of Microbiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Lei
Yan-Chang
YC
Hao
You-Hua
YH
Chen
Shan-Shan
SS
Wu
Wen
W
Yang
Dong-Liang
DL
Lu
Meng-Ji
MJ
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D015536:Down-Regulation; D016174:Hepacivirus / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D050832:Interferon-Stimulated Gene Factor 3 / Q000235:genetics / Q000378:metabolism*; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology*; D008113:Liver Neoplasms / Q000473:pathology; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000378:metabolism; D050795:STAT2 Transcription Factor / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D050832:Interferon-Stimulated Gene Factor 3; D016898:Interferon-alpha; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D050795:STAT2 Transcription Factor; D014758:Viral Core Proteins; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase