title: Direct capping of human pulps with a dentin bonding system and calcium hydroxide: an immunohistochemical analysis.
abstract: Pulp capping is a treatment where a protective agent is applied to an exposed pulp to allow the maintenance of its vitality and function. The present study analyzed the immunohistochemical expression of fibronectin and type III collagen in human dental pulps submitted to direct pulp capping with calcium hydroxide [Ca(OH)2] or the Single Bond adhesive system (SBAS). The results demonstrated that both proteins were not expressed in the SBAS group, although in the group capped with Ca(OH)2 a diffuse labeling in the extracellular matrix was initially observed, followed by a late expression in the odontoblast-like layer and beneath the dentin bridge. It seems that application of adhesive systems in direct contact with healthy pulps will not lead to expression of proteins that are believed to be essential for pulpal repair. Moreover, Ca(OH)2 showed good biocompatibility properties with the dental pulp tissue, inducing the expression of reparative molecules, and therefore remains the material of choice for the treatment of accidental pulp exposures.
authors.affiliation: Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Fernandes
authors.forename: Gerluza A B
authors.initials: GA
authors.lastname: Silva
authors.forename: Nelson
authors.initials: N
authors.lastname: Lopes
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Napimoga
authors.forename: Bruno B
authors.initials: BB
authors.lastname: Benatti
authors.forename: José B
authors.initials: JB
authors.lastname: Alves
mesh_terms: D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000627:therapeutic use; D002126:Calcium Hydroxide / Q000627:therapeutic use*; D024061:Collagen Type III / Q000096:biosynthesis; D003782:Dental Pulp / Q000378:metabolism*; D003785:Dental Pulp Capping / Q000379:methods*; D003809:Dentin, Secondary / Q000378:metabolism; D017220:Dentin-Bonding Agents / Q000627:therapeutic use*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005353:Fibronectins / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D019279:Resin Cements / Q000627:therapeutic use
chemical_list: D024061:Collagen Type III; D017220:Dentin-Bonding Agents; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019279:Resin Cements; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D002126:Calcium Hydroxide