Reptin and Pontin function antagonistically with PcG and TrxG complexes to mediate Hox gene control.
Pontin (Pont) and Reptin (Rept) are paralogous ATPases that are evolutionarily conserved from yeast to human. They are recruited in multiprotein complexes that function in various aspects of DNA metabolism. They are essential for viability and have antagonistic roles in tissue growth, cell signalling and regulation of the tumour metastasis suppressor gene, KAI1, indicating that the balance of Pont and Rept regulates epigenetic programmes critical for development and cancer progression. Here, we describe Pont and Rept as antagonistic mediators of Drosophila Hox gene transcription, functioning with Polycomb group (PcG) and Trithorax group proteins to maintain correct patterns of expression. We show that Rept is a component of the PRC1 PcG complex, whereas Pont purifies with the Brahma complex. Furthermore, the enzymatic functions of Rept and Pont are indispensable for maintaining Hox gene expression states, highlighting the importance of these two antagonistic factors in transcriptional output.
Diop
Soda Balla
SB
Institut de Biologie du Développement de Marseille-Luminy, CNRS UMR6216/Université de la Méditerranée, Parc Scientifique de Luminy, Case 907, 13288 Marseille Cedex 9, France.
Bertaux
Karine
K
Vasanthi
Dasari
D
Sarkeshik
Ali
A
Goirand
Benjamin
B
Aragnol
Denise
D
Tolwinski
Nicholas S
NS
Cole
Michael D
MD
Pradel
Jacques
J
Yates
John R
JR
Mishra
Rakesh K
RK
Graba
Yacine
Y
Saurin
Andrew J
AJ
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000302:isolation & purification / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004265:DNA Helicases / Q000302:isolation & purification / Q000378:metabolism*; D029721:Drosophila Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D063146:Polycomb-Group Proteins; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D015534:Trans-Activators / Q000302:isolation & purification / Q000378:metabolism
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; C086469:brm protein, Drosophila; C486546:rept protein, Drosophila; C512571:trx protein, Drosophila; D004265:DNA Helicases; C504804:pont protein, Drosophila