A rapid and efficient determination of natural estrogens in soils by pressurised liquid extraction and gas chromatography-mass spectrometry.
We present a new analytical procedure for the extraction and determination of natural estrogens in soils based on pressurised liquid extraction and GC-MS determination. After testing twelve solvents, acetone proved to be the most efficient extractant. The optimum extraction temperature is 60 degrees C. Soil extracts have to be purified and concentrated by C-18 solid phase extraction. The dried extracts are derivatised by N-methyl-N-(trimethylsilyl)trifluoro-acetamide before measurement by GC-MS. Recoveries of 79-103% with relative standard deviations <or=13% document reliability of this method. Further, rapidity of extraction and a low detection limit (1 ng kg(-1)) represent an improvement of estrogen analysis in soils compared to established procedures.
Beck
Josefine
J
Josefine Beck, Lehrstuhl für Bodenkunde, Department für Okologie, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Technische Universität München, D-85350 Freising-Weihenstephan, Germany. beckj@wzw.tum.de
Totsche
Kai Uwe
KU
Kögel-Knabner
Ingrid
I
D000096:Acetone / Q000737:chemistry; D004967:Estrogens / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D012987:Soil / Q000032:analysis*
D004967:Estrogens; D012987:Soil; D000096:Acetone