A new ischemic model using a radiofrequency wire electrode in a rabbit hindlimb.
The purpose of this study was to establish an ischemic rabbit hindlimb model using a radiofrequency (RF) wire electrode. We inserted a polytetrafluoroethylene-coated wire with a 2-cm exposed tip into the left superficial femoral artery of seven New Zealand white rabbits and performed RF ablation (RFA) while pulling the wire back. We assessed the clinical findings, angiography, computed tomography perfusion, and permeability surface until 6 weeks after RFA. The angiography demonstrated complete obstruction from the proximal external iliac artery to the distal superficial femoral artery and showed a gradual increment in the angiogenic score, which represents the degree of angiogenesis (r = 0.86, p < 0.0001). The left-to-right ratios of the computed tomography perfusion and permeability surface were significantly reduced after 4 days (p < 0.05), and then they gradually increased with time. We conclude that endovascular RFA using an RF wire electrode is a reproducible and measurable way to create an ischemic rabbit hindlimb model.
Baik
Hye Won
HW
Department of Radiology, Chung-Ang University Hospital, Chung-Ang University College of Medicine, 224-1 Heukseok-dong, Dongjak-gu, Seoul, 156-755, South Korea. hyewonbaik@hanmail.net
Kwak
Byung Kook
BK
Shim
Hyung Jin
HJ
Kim
Yang Soo
YS
Lee
Jong Beom
JB
Kim
Kun Sang
KS
D000792:Angiography / Q000379:methods; D000818:Animals; D001707:Biopsy, Needle; D017115:Catheter Ablation / Q000379:methods*; D004195:Disease Models, Animal; D004567:Electrodes, Implanted; D006614:Hindlimb / Q000098:blood supply* / Q000000981:diagnostic imaging; D007150:Immunohistochemistry; D007511:Ischemia / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery*; D008297:Male; D011336:Probability; D011817:Rabbits; D011897:Random Allocation; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D036542:Tomography, Spiral Computed / Q000379:methods