title: Mapping IgE-binding epitopes of Ric c 1 and Ric c 3, allergens from Ricinus communis, by mast cell degranulation assay.
abstract: Ric c 1 and Ric c 3 are the major castor bean allergens. In order to identify continuous IgE-epitopes in Ric c 1 and Ric c 3, pools of sera from rats immunized with a pool of 2S albumin from these seeds, Ric c 1 and Ric c 3 overlapping synthetic peptides, were used to screen for IgE-binding epitopes. The allergenic properties were monitored by mast cell degranulation assays, histamine quantification and human-IgE binding. Large and small chains isolated from these proteins present allergenic properties. Four continuous epitopes were identified in Ric c 3 and two in Ric c 1. This knowledge may allow the induction of protective antibody responses to antagonize the IgE recognition.
authors.affiliation: Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Darcy Ribeiro, Brazil.
authors.forename: S P
authors.initials: SP
authors.lastname: Felix
authors.forename: R O
authors.initials: RO
authors.lastname: Mayerhoffer
authors.forename: R A
authors.initials: RA
authors.lastname: Damatta
authors.forename: M A
authors.initials: MA
authors.lastname: Verícimo
authors.forename: V V
authors.initials: VV
authors.lastname: Nascimento
authors.forename: O L T
authors.initials: OL
authors.lastname: Machado
mesh_terms: D055516:2S Albumins, Plant; D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D015550:Cell Degranulation; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D010940:Plant Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012278:Ricinus / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D055516:2S Albumins, Plant; C541551:2S storage protein, Ricinus communis; D000485:Allergens; D052179:Antigens, Plant; D000939:Epitopes; D010455:Peptides; D010940:Plant Proteins; D007073:Immunoglobulin E