[Expression of Survivin mRNA and Caspase-3 mRNA and their correlation in tongue squamous cell carcinoma].
OBJECTIVE
To investigate the expression of Survivin mRNA and Caspase-3 mRNA in tongue squamous cell carcinoma(TSCC), and clarify their significance and correlation in TSCC.


METHODS
In situ hybridization technique was employed to detect the expression of Survivin mRNA and Caspase-3 mRNA in 10 normal lingual mucous membranes (NLMM) and 45 TSCCs. Data was analyzed with SPSS13.0 software package for Chi-square test and Fisher's exact test, and Spearman rank correlation was used for analysis of their correlation.


RESULTS
The positive staining rate of Survivin mRNA in TSCC was 55.6%, and significantly higher than that in normal control group (P<0.01). The positive staining rate of Caspase-3 mRNA in TSCC was 42.2%, and significantly lower than that in normal control group 80.0%(P<0.05). Overexpression of Survivin mRNA and underexpression of Caspase-3 mRNA were related to the pathological grade, cervical lymphatic metastasis of TSCC (P<0.05) and closely related to TNM stage (P<0.01). There was a negative relation between them, and the Spearman correlation coefficient r was -0.412, P=0.005.


CONCLUSIONS
The higher expression of Survivin mRNA takes part in inhibiting Caspase-3 mRNA expression, which correlates with carcinogenesis and progress of TSCC.
Wang
Zhi-ming
ZM
Department of Stomatology, Shengjing Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. wangzhiming_cmu@yahoo.com.cn
Zhao
Zhi-guo
ZG
Guan
Xin-ming
XM
Liu
Fang
F
Zhang
Li-ping
LP
D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D053148:Caspase 3 / Q000096:biosynthesis*; D006801:Humans; D008207:Lymphatic Metastasis; D009061:Mouth Mucosa; D011379:Prognosis; D012333:RNA, Messenger; D014062:Tongue Neoplasms / Q000378:metabolism*
D012333:RNA, Messenger; D053148:Caspase 3