Synthesis of biodegradable and electroactive multiblock polylactide and aniline pentamer copolymer for tissue engineering applications.
To obtain one biodegradable and electroactive polymer as the scaffold for tissue engineering, the multiblock copolymer PLAAP was designed and synthesized with the condensation polymerization of hydroxyl-capped poly( l-lactide) (PLA) and carboxyl-capped aniline pentamer (AP). The PLAAP copolymer exhibited excellent electroactivity, solubility, and biodegradability. At the same time, as one scaffold material, PLAAP copolymer possesses certain mechanical properties with the tensile strength of 3 MPa, tensile Young 's modulus of 32 MPa, and breaking elongation rate of 95%. We systematically studied the compatibility of PLAAP copolymer in vitro and proved that the electroactive PLAAP copolymer was innocuous, biocompatible, and helpful for the adhesion and proliferation of rat C6 cells. Moreover, the PLAAP copolymer stimulated by electrical signals was demonstrated as accelerating the differentiation of rat neuronal pheochromocytoma PC-12 cells. This biodegradable and electroactive PLAAP copolymer thus possessed the properties in favor of the long-time application in vivo as nerve repair scaffold materials in tissue engineering.
Huang
Lihong
L
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Zhuang
Xiuli
X
Hu
Jun
J
Lang
Le
L
Zhang
Peibiao
P
Wang
Yu
Y
Chen
Xuesi
X
Wei
Yen
Y
Jing
Xiabin
X
D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001673:Biodegradation, Environmental; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004558:Electric Stimulation; D006358:Hot Temperature; D008422:Materials Testing; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D016716:PC12 Cells; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D012995:Solubility; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D000814:Aniline Compounds; D001672:Biocompatible Materials; C528060:PLAAP polymer; D011091:Polyesters; C033616:poly(lactide); C023650:aniline; D004121:Dimethyl Sulfoxide