Screening for novel binding proteins interacting with human papillomavirus type 18 E6 oncogene in the Hela cDNA library by yeast two-hybrid system.
To screen for novel binding proteins interacting with high-risk HPV 18 E6 oncogene, the strain AH109 was transformed with pGBKT7-HPV18 E6 plasmid, and subsequent transference was utilized to screen for interacting proteins with HPV 18 E6 in human Hela cDNA library. HPV18 E6 mRNA was expressed in yeast and there was no self-activation and toxicity in AH109. Seven proteins that interacted with HPV18 E6, including transmembrane protein 87B, phosphonoformate immuno-associated protein 5, vimentin, KM-HN-1 protein, dedicator of cytokinesis 7, vaccinia related kinase 2 and a hypothetical protein, were identified. It was suggested that yeast two-hybrid system is an efficient for screening interacting proteins. The high-risk HPV 18 E6 oncogene may interact with the proteins, which may be associated with signal transduction and transcriptional control, epithelial cell invasion and migration, as well as humoral and cellular immune etc. This investigation provides functional clues for further exploration of potential oncogenesis targets for cancer biotherapy.
Li
Shuang
S
Cancer Biology Center, Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. lee5190008@126.com
Liu
Ping
P
Xi
Ling
L
Jiang
Xuefeng
X
Zhou
Jianfeng
J
Wang
Shixuan
S
Meng
Li
L
Lu
Yunping
Y
Ma
Ding
D
D002352:Carrier Proteins / Q000235:genetics; D002465:Cell Movement; D048749:Cytokinesis; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D005821:Genetic Techniques; D006367:HeLa Cells; D052161:Human papillomavirus 18 / Q000235:genetics*; D006801:Humans; D009361:Neoplasm Invasiveness; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis* / Q000235:genetics*; D011485:Protein Binding; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral