Generation of HIV-1-based lentiviral vector particles.
This unit presents protocols outlining the methodology and techniques involved in the construction and application of HIV-1-based lentiviral vector systems. Also described are procedures that can be used to concentrate and purify high-titer recombinant lentiviral vector preparations, as well as protocols for transduction of adherent and suspension cells.
Ramezani
Ali
A
American Red Cross, Rockville, Maryland, USA.
Hawley
Robert G
RG
D002460:Cell Line; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange / Q000379:methods; D003673:Defective Viruses / Q000235:genetics; D015750:Genes, gag; D015756:Genes, tat; D005822:Genetic Vectors / Q000235:genetics* / Q000302:isolation & purification; D054303:HIV Reverse Transcriptase / Q000032:analysis; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D007668:Kidney; D011107:Polylysine; D014161:Transduction, Genetic / Q000379:methods; D014461:Ultracentrifugation / Q000379:methods
D007202:Indicators and Reagents; D011107:Polylysine; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase