title: A mouse model for Chikungunya: young age and inefficient type-I interferon signaling are risk factors for severe disease.
abstract: Chikungunya virus (CHIKV) is a re-emerging arbovirus responsible for a massive outbreak currently afflicting the Indian Ocean region and India. Infection from CHIKV typically induces a mild disease in humans, characterized by fever, myalgia, arthralgia, and rash. Cases of severe CHIKV infection involving the central nervous system (CNS) have recently been described in neonates as well as in adults with underlying conditions. The pathophysiology of CHIKV infection and the basis for disease severity are unknown. To address these critical issues, we have developed an animal model of CHIKV infection. We show here that whereas wild type (WT) adult mice are resistant to CHIKV infection, WT mouse neonates are susceptible and neonatal disease severity is age-dependent. Adult mice with a partially (IFN-alpha/betaR(+/-)) or totally (IFN-alpha/betaR(-/-)) abrogated type-I IFN pathway develop a mild or severe infection, respectively. In mice with a mild infection, after a burst of viral replication in the liver, CHIKV primarily targets muscle, joint, and skin fibroblasts, a cell and tissue tropism similar to that observed in biopsy samples of CHIKV-infected humans. In case of severe infections, CHIKV also disseminates to other tissues including the CNS, where it specifically targets the choroid plexuses and the leptomeninges. Together, these data indicate that CHIKV-associated symptoms match viral tissue and cell tropisms, and demonstrate that the fibroblast is a predominant target cell of CHIKV. These data also identify the neonatal phase and inefficient type-I IFN signaling as risk factors for severe CHIKV-associated disease. The development of a permissive small animal model will expedite the testing of future vaccines and therapeutic candidates.
authors.affiliation: Groupe "Microorganismes et Barrières de l'Hôte", Institut Pasteur, Paris, France.
authors.forename: Thérèse
authors.initials: T
authors.lastname: Couderc
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chrétien
authors.forename: Clémentine
authors.initials: C
authors.lastname: Schilte
authors.forename: Olivier
authors.initials: O
authors.lastname: Disson
authors.forename: Madly
authors.initials: M
authors.lastname: Brigitte
authors.forename: Florence
authors.initials: F
authors.lastname: Guivel-Benhassine
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Touret
authors.forename: Georges
authors.initials: G
authors.lastname: Barau
authors.forename: Nadège
authors.initials: N
authors.lastname: Cayet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schuffenecker
authors.forename: Philippe
authors.initials: P
authors.lastname: Desprès
authors.forename: Fernando
authors.initials: F
authors.lastname: Arenzana-Seisdedos
authors.forename: Alain
authors.initials: A
authors.lastname: Michault
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Albert
authors.forename: Marc
authors.initials: M
authors.lastname: Lecuit
mesh_terms: D000328:Adult; D000367:Age Factors; D018354:Alphavirus Infections / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D000831:Animals, Newborn; D021461:Animals, Outbred Strains; D045744:Cell Line, Tumor; D002646:Chikungunya virus / Q000472:pathogenicity / Q000502:physiology*; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007370:Interferon Type I / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008136:Longevity; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015398:Signal Transduction; D014709:Vero Cells; D019562:Viral Load; D014779:Virus Replication
chemical_list: D007370:Interferon Type I