title: Production of monoclonal antibody supernatant and ascites fluid.
abstract: Procedures detailing the production of monoclonal antibody supernatants, including the production of larger amounts (liters) are presented in this unit. An alternate protocol for large-scale production of hybridomas or cells (e.g., for isolation of cellular proteins) involves a similar procedure. A method for producing and obtaining ascites fluid containing the monoclonal antibody is also presented.
authors.affiliation: University of California School of Medicine, San Francisco, California, USA.
authors.forename: W M
authors.initials: WM
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000378:metabolism*; D001201:Ascites / Q000276:immunology*; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D006825:Hybridomas; D007158:Immunologic Techniques
chemical_list: D000911:Antibodies, Monoclonal