Polony DNA sequencing.
Polony DNA sequencing provides an inexpensive, accurate, high-throughput way to resequence genomes of interest by comparison to a reference genome. Mate-paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on a microscope slide. Each sequencing run results in millions of 26-bp reads that can be aligned to the reference genome, allowing the identification of differences between sequences.
Porreca
Gregory J
GJ
Harvard Medical School, Boston, Massachusetts, USA.
Shendure
Jay
J
Church
George M
GM
D000818:Animals; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods / Q000639:trends; D017422:Sequence Analysis, DNA / Q000379:methods* / Q000639:trends; D054928:Validation Studies as Topic