title: BDNF preserves the dendritic morphology of alpha and beta ganglion cells in the cat retina after optic nerve injury.
abstract: OBJECTIVE
  To examine whether brain-derived neurotrophic factor (BDNF), a potent neuroprotectant in the mammalian retina, also plays a role in preserving the dendritic integrity of the surviving ganglion cells after optic nerve injury.
  
  
  METHODS
  Single ganglion cells from cats that underwent unilateral optic nerve crush and received no treatment or nerve crush combined with intravitreous treatment of the affected eye with BDNF were labeled intracellularly, reconstructed using confocal microscopy, and compared quantitatively.
  
  
  RESULTS
  Optic nerve injury produced a significant decrease in the soma, dendritic field size, and dendritic complexity of alpha cells. beta Cells also displayed a significant decrease in soma size, but their dendritic fields were not affected as severely as those of alpha cells. Intravitreous treatment of the eye with BDNF at the time of injury preserved the normal somal and dendritic morphologies of both alpha and beta cells.
  
  
  CONCLUSIONS
  BDNF, in addition to promoting ganglion cell survival, plays an important role in preserving the somal and dendritic morphologies of the surviving ganglion cells, a necessary precursor to maintaining normal visual function. Ganglion cells, however, are not created equal with respect to their responses to nerve injury or to treatment of the eye with BDNF. Thus, development of effective treatment strategies for preserving ganglion cell function in optic nerve-related diseases mandates a clearer understanding of the cellular response characteristics of the specific neurons involved.
authors.affiliation: Department of Physiology, Michigan State University, East Lansing, Michigan 48824, USA. weberar@msu.edu
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Weber
authors.forename: Christine D
authors.initials: CD
authors.lastname: Harman
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002415:Cats; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D003712:Dendrites / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020221:Optic Nerve Injuries / Q000150:complications / Q000188:drug therapy*; D011994:Recombinant Proteins / Q000494:pharmacology; D012162:Retinal Degeneration / Q000209:etiology / Q000517:prevention & control; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000473:pathology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D011994:Recombinant Proteins