Characterisation of electron currents generated by the human neutrophil NADPH oxidase.
Electron transport by the human neutrophil NADPH oxidase is an important microbicidal weapon for phagocytes. The electron current (I(e)) generated by the neutrophil NADPH oxidase is poorly characterised due to the lack of appropriate electrophysiological data. In this study, I fully characterise the neutrophil generated I(e) when the NADPH oxidase is activated by NADPH and GTPgammaS. The neutrophil I(e) was markedly voltage-dependent in the entire voltage range in comparison to those electron currents measured after chloride was removed from the external bath solution. The difference in I(e) measured in chloride free conditions was not due to a change in the activation kinetics of voltage-gated proton channels. The I(e) depolarises the neutrophil plasma membrane at a rate of 2.3 Vs(-1) and this depolarisation was opposed when voltage-gated proton channels are activated. 3 mM ZnCl(2) depolarised the membrane potential to +97.8+/-2.5 mV (n=4), and this depolarisation was abolished after NADPH oxidase inhibition.
Ahluwalia
Jatinder
J
Leukocyte and Ion Channel Research Laboratory, School of Health and Biosciences, University of East London, Stratford Campus, London E15 4LZ, UK.
D002463:Cell Membrane Permeability / Q000502:physiology*; D002478:Cells, Cultured; D004553:Electric Conductivity; D004579:Electron Transport; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology*; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology*
D019255:NADPH Oxidases