title: 15,16-dihydrotanshinone I, a major component from Salvia miltiorrhiza Bunge (Dansham), inhibits rabbit platelet aggregation by suppressing intracellular calcium mobilization.
abstract: Radix Salviae miltiorrhiza (RSM, 'Dansham' in Korea, 'Danshen' in Chinese), the root of Salviae miltiorrhiza Bunge (Labiate) has been used as Chinese fork medicine for the treatment of cardiovascular diseases such as angina pectoris, coronary heart disease, myocardial infarction, and hypertension. In the present study, we evaluated the inhibitory effects of 15,16-Dihydrotanshinone I, one of the major ingredients of Salvia miltiorrhiza Bunge, on platelet aggregation, with elucidation of its mechanisms of action. 15,16-Dihydrotanshinone I concentration-dependently inhibited collagen-induced aggregation of rabbit washed platelets with IC50 of 8.7+/-5.6 microM, the potency being about seven-fold greater than EGCG, an active Green tea catechin component (IC50: 56.6+/-48.7 microM). 15,16-Dihydrotanshinone I significantly inhibited the intracellular calcium ([Ca2+]i) mobilization in a concentration-dependent manner. 15,16-dihdydrotanshinone I also significantly suppressed collagen (50 microg/mL)-induced liberation of [3H]Arachidonic acid from [3H]Arachidonic acid-incorporated rabbit platelet. In addition, 15,16-Dihydrotanshinone I at 50 microM slightly but significantly inhibited collagen-induced production of thromboxane B2. These results indicate that 15,16-Dihydrotanshinone I exert potent anti-platelet activity via suppression of [Ca2+]i mobilization and arachidonic acid liberation.
authors.affiliation: Department of Applied Biochemistry, Division of Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea.
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Sung-Hun
authors.initials: SH
authors.lastname: Lee
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Yang
authors.forename: Jung-Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Min-Jin
authors.initials: MJ
authors.lastname: Song
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Ryu
authors.forename: Hun-Jong
authors.initials: HJ
authors.lastname: Chung
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Won
authors.forename: Chang-Soo
authors.initials: CS
authors.lastname: Lee
authors.forename: Suk-Hyung
authors.initials: SH
authors.lastname: Kwon
authors.forename: Yeo-Pyo
authors.initials: YP
authors.lastname: Yun
authors.forename: Wahn-Soo
authors.initials: WS
authors.lastname: Choi
authors.forename: Hwa-Sup
authors.initials: HS
authors.lastname: Shin
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D003094:Collagen / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005663:Furans; D066298:In Vitro Techniques; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors; D011809:Quinones; D011817:Rabbits; D027544:Salvia / Q000737:chemistry*; D013917:Thrombin / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood
chemical_list: D005663:Furans; D010616:Phenanthrenes; D010975:Platelet Aggregation Inhibitors; D011809:Quinones; D016718:Arachidonic Acid; D013929:Thromboxane B2; C000713095:dihydrotanshinone I; D003094:Collagen; D013917:Thrombin; D002118:Calcium