title: Ibuprofen metabolite profiling using a combination of SPE/column-trapping and HPLC-micro-coil NMR.
abstract: Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC-nuclear magnetic resonance (HPLC-NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold.
authors.affiliation: Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Danijel
authors.initials: D
authors.lastname: Djukovic
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Appiah-Amponsah
authors.forename: Narasimhamurthy
authors.initials: N
authors.lastname: Shanaiah
authors.forename: G A Nagana
authors.initials: GA
authors.lastname: Gowda
authors.forename: Ian
authors.initials: I
authors.lastname: Henry
authors.forename: Mike
authors.initials: M
authors.lastname: Everly
authors.forename: Brian
authors.initials: B
authors.lastname: Tobias
authors.forename: Daniel
authors.initials: D
authors.lastname: Raftery
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007052:Ibuprofen / Q000378:metabolism* / Q000652:urine; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D013607:Tablets; D013997:Time Factors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D013607:Tablets; D007052:Ibuprofen