High levels of dietary unsaturated fat decrease alpha-tocopherol content of whole body, liver, and plasma of chickens without variations in intestinal apparent absorption.
An experiment was designed to assess the effect of dietary unsaturated fat inclusion level on alpha-tocopherol apparent absorption and deposition in broiler chickens at 2 ages (20 and 39 d). The dietary fat was a mixture of linseed and fish oil, rich in polyunsaturated fatty acids (PUFA). The experimental treatments were the result of 4 levels of supplementation with alpha-tocopheryl acetate (0, 100, 200, and 400 mg/kg; E0, E100, E200, and E400 treatments, respectively) and 4 dietary oil inclusion levels (2, 4, 6, and 8%; O2, O4, O6, and O8 treatments respectively). Almond husk was used as an energy dilutor in the high-fat diets. Apparent absorption of total fatty acids was high in all treatments averaging 88% and was higher with high fat dietary inclusion level. alpha-Tocopheryl acetate hydrolysis and apparent absorption of alpha-tocopherol were similar in both ages and were not affected by fat inclusion level, except for a reduction of the absorption in the low-fat diet (O2) in the E100 treatment at 20 d of age. Despite this lack of differences in hydrolysis and absorption, higher-fat PUFA diets induced lower concentrations of free alpha-tocopherol in the excreta, at high alpha-tocopherol doses, suggesting an increase in the destruction of alpha-tocopherol by lipid oxidation in the gastrointestinal tract. Similarly, total and hepatic alpha-tocopherol deposition was lower in the birds fed high-PUFA diets in the E200- and E400-supplemented birds, possibly due to a destruction of vitamin E when protecting these PUFA from lipid peroxidation. alpha-Tocopherol concentration in liver and, to a lesser extent, in plasma was a useful indicator of the degree of response of this vitamin to different factors that can affect its bioavailability; however, in the present experiment, CV were too high to use liver and plasma concentrations as estimators of total body vitamin E.
Villaverde
C
C
Grup de Recerca en Nutrició, Maneig i Benestar Animal, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain.
Baucells
M D
MD
Manzanilla
E G
EG
Barroeta
A C
AC
D000222:Adaptation, Physiological; D000375:Aging / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001682:Biological Availability; D002645:Chickens / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005395:Fish Oils; D006868:Hydrolysis; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology; D008043:Linseed Oil; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D011897:Random Allocation; D024502:alpha-Tocopherol / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics
D000975:Antioxidants; D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D008043:Linseed Oil; D024502:alpha-Tocopherol