Osterix overexpression enhances osteoblast differentiation of muscle satellite cells in vitro.
Muscle satellite cells have long been considered a distinct myogenic lineage responsible for postnatal growth, repair and maintenance of skeletal muscle. Recent studies have demonstrated that they are multi-potential. Osterix (Osx), a novel zinc-finger-containing transcription factor of the sp family, is required for osteoblast differentiation and bone formation. It was hypothesized that Osx overexpression would enhance osteoblast differentiation of muscle satellite cells in vitro. Recombinant adenovirus-mediated Osx gene (Ad-Osx) was constructed and used to transfect muscle satellite cells. Osx overexpression inhibited myogenesis, as demonstrated by suppression of MyoD and myogenin mRNA levels and reduced myotube formation. Muscle satellite cells transduced with Ad-Osx exhibited apparent osteoblast differentiation as determined by the expression of related osteoblastic genes, increased activity of alkaline phosphatase and the formation of mineralized nodules. These results confirmed the ability of Osx to enhance osteoblast differentiation of muscle satellite cells in vitro, and the competence of muscle satellite cells as promising seed cells for bone tissue engineering.
Sun
S
S
Department of Oral and Maxillofacial Surgery, School of Stomatology, Tongji University, Shanghai, China.
Wang
Z
Z
Hao
Y
Y
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D015870:Gene Expression; D058574:Integrin-Binding Sialoprotein; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D024510:Muscle Development; D017570:MyoD Protein / Q000037:antagonists & inhibitors; D018008:Myogenin / Q000037:antagonists & inhibitors; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000235:genetics*; D053495:Osteopontin / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism; D012795:Sialoglycoproteins / Q000096:biosynthesis; D023822:Tissue Engineering / Q000379:methods*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D014162:Transfection; D015854:Up-Regulation
D024042:Collagen Type I; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D017570:MyoD Protein; D018008:Myogenin; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; C495181:Sp7 protein, rat; D014157:Transcription Factors; D015675:Osteocalcin; D053495:Osteopontin