A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides.
We have developed a reversed-phase high-performance liquid chromatography pulsed amperometric detection (RP-HPLC-PAD) method for the determination of glycosides. It is sensitive, repeatable, and selective without the pretreatment step. Ginsenosides were separated completely in 50 min using an water-acetonitrile gradient as the eluent and detected by PAD under NaOH alkaline conditions. The ginsenoside detection limit (S/N=3) was 0.02-0.07 ng and the quantification limit (S/N=10) was 0.1-0.2 ng. The coefficient of linear regression was 0.9984-0.9998 for concentrations between 1 and 50 microg/mL. The intra- and inter-day precision (RSD) was less than 6.35% in Ginseng Radix and Shy-jiun-tzyy-tang extracts. The average recoveries from Ginseng Radix and Shy-jiun-tzyy-tang extracts were 98.19-105.45% and 96.89-102.22%, respectively.
Kwon
Ha-Jeong
HJ
Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, 1 Hoegi-dong, Dongdaemoon-gu, Seoul 130-701, South Korea.
Jeong
Ji-Seon
JS
Lee
Yong-Moon
YM
Hong
Seon-Pyo
SP
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D005894:Panax / Q000737:chemistry; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides