Effect of Bushenantai recipe on the expression of endometrial LIF in mice with embryonic implantation dysfunction.
In order to observe the effect of Bushenantai recipe on the expression of endometrial leukemia-inhibitory factor (LIF) in mice with embryonic implantation dysfunction (EID), 120 Kunming mice post coition were randomized into three groups: normal control group, model group and traditional Chinese medicine group (TCM group) (n=40 in each group). Uterus was collected on the pregnancy day (Pd) 4, 5, 6 after an intravenous injection of Evan's blue. The endometrium was dyed by Evan's blue and the mean points of response were observed on Pd 5. The expression of LIF mRNA and protein was detected by RT-PCR and immunohistochemistry respectively and analyzed statistically by image system. The results showed that the number of implantation sites in model group was remarkably less than in normal control group and TCM group. There was no significant difference between normal control group and TCM group. The expression of LIF mRNA and protein in model group was delayed. Bushenantai recipe could increase the expression of LIF mRNA and protein in endometria of mice with EID. It was suggested that Bushenantai recipe could improve embryo implantation of mice with EID by promoting the endometrial LIF expression and endometrial decidualization.
Zhang
Mingmin
M
Department of Integrated Traditional Chinese and Western Medicine, Huazhong University of Science and Technology, Wuhan 430030, China. mmzhang@tjh.tjmu.edu.cn
Huang
Yuqin
Y
Zhu
Guijin
G
Huang
Guangying
G
Dong
Liping
L
Zhang
Jinjin
J
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D053605:Leukemia Inhibitory Factor / Q000096:biosynthesis* / Q000235:genetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008954:Models, Biological; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D010936:Plant Extracts; D012333:RNA, Messenger