Immunization of mice.
In the protocols in this unit, antigen is prepared for injection either by emulsifying an antigen solution with Freunds adjuvant or by homogenizing a polyacrylamide gel slice containing the protein antigen. Mice are immunized at 2- to 3-week intervals. Test bleeds are collected 7 days after each booster immunization to monitor serum antibody levels. Mice are chosen for hybridoma fusions when a sufficient antibody titer is reached.
Fuller
S A
SA
ADI Diagnostics, Rexdale, Ontario.
Takahashi
M
M
Hurrell
J G
JG
D000818:Animals; D000906:Antibodies / Q000097:blood*; D007114:Immunization / Q000379:methods*; D051379:Mice
D000906:Antibodies