Detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque samples from Brazilian preschool children by polymerase chain reaction.
The purposes of this study were to detect S. mutans and S. sobrinus by polymerase chain reaction (PCR) amplification, and to relate their presence to the incidence of dental caries in 42 Brazilian preschool children. Dental plaque samples were collected from the cervical margin of all erupted teeth of 5-6 years old children with primary dentition, using a sterile explorer. Examination of the dmft (decayed, missing, filled teeth) index, performed following the World Health Organization (WHO) caries diagnostic criteria, showed a 2.71 score. Prevalence of S. mutans and S. sobrinus was respectively, of 85.7% and 14.3%; no dental plaque sample was either positive or negative for both bacterial species. Children harboring either S. mutans or S. sobrinus presented the same caries prevalence. PCR showed good discriminative ability for differentiation between these species, and suggested that it is a technique suitable for epidemiological studies on mutans streptococci.
Franco e Franco
Teresa Cristina Costa
TC
School of Dentistry, Educational Foundation of Barretos, Barretos, SP, Brazil.
Amoroso
Patrícia
P
Marin
José Moacir
JM
de Avila
Fernando Antonio
FA
D001938:Brazil; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D004245:DMF Index; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012737:Sex Factors; D013295:Streptococcus mutans / Q000235:genetics / Q000302:isolation & purification*; D016986:Streptococcus sobrinus / Q000235:genetics / Q000302:isolation & purification*; D019237:Tooth Cervix / Q000382:microbiology; D014094:Tooth, Deciduous / Q000382:microbiology
D004269:DNA, Bacterial