Androgens modulate testicular androgen production in African catfish (Clarias gariepinus) depending on the stage of maturity and type of androgen.
Previous work showed that androgen treatment suppressed testicular steroidogenesis in juvenile African catfish Clarias gariepinus. Similar to other vertebrates, however, circulating androgen levels increase during puberty in catfish. We therefore studied if androgen-induced inhibition of androgen production decreases during sexual maturation. As in other vertebrates, testosterone (T) is found in the circulation in fish but typically, 11-ketotestosterone (11-KT) is the quantitatively dominating androgen. In previous studies with juvenile catfish, these two androgens showed different biological activities as regards spermatogenesis or pituitary hormone production, but were equally effective in suppressing testicular steroidogenesis. Hence, the second question we studied was if the two types of androgens show distinct effects on the steroidogenic system in pubertal or adult males. The inhibitory effect of 11-KT on the testicular steroidogenic capacity waned with progressing sexual maturation, while T-mediated inhibition remained strong until adulthood reducing the in vitro steroid production 4- to 10-fold. However, the gonadotropin responsiveness of testicular tissue was not compromised and expression of testicular gonadotropin receptors did not respond differently to the two androgens. We conclude that the selective disappearance of the inhibitory effect of 11-KT contributes to allowing the pubertal increase of the plasma level of this androgen.
Schulz
Rüdiger W
RW
Utrecht University, Science Faculty, Department of Biology, Endocrinology & Metabolism Section, Kruyt Building Room Z-203, Padualaan 8, NL-3584 CH Utrecht, The Netherlands. r.w.schulz@uu.nl
Liemburg
Maurice
M
García-López
Angel
A
Dijk
Wytske van
Wv
Bogerd
Jan
J
D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000494:pharmacology; D000818:Animals; D002397:Catfishes / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D006065:Gonadotropins, Pituitary; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011967:Receptors, Gonadotropin / Q000378:metabolism; D012741:Sexual Maturation / Q000187:drug effects / Q000502:physiology*; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000728:Androgens; D006065:Gonadotropins, Pituitary; D012333:RNA, Messenger; D011967:Receptors, Gonadotropin; C077886:gonadotropin II beta subunit, catfish; D005576:Colforsin; D013739:Testosterone; D000735:Androstenedione; D007986:Luteinizing Hormone; C003600:11-ketotestosterone