Determination of IGF-I in horse plasma by LC electrospray ionisation mass spectrometry.
The insulin-like-growth factor (IGF-I) peptide is considered to be the main indirect marker for growth hormone administration (GH) in a horse. Further to a previous investigation on measurement of IGF-I in plasma samples by mass spectrometry, this study focuses on quantitative and qualitative analysis of intact IGF-I in horse plasma. First, protein-transposing software has been developed for IGF-I to facilitate its quantification by HPLC-electrospray-ion-trap mass spectrometry. Second, product-ion scan experiments on IGF-I have been conducted on standard samples, non-fortified equine plasma samples, fortified plasma samples, and equine GH post-administration samples. This "top-down" approach method enables characterisation of fragment ions corresponding to the carboxy terminal end, which can be useful for the confirmation of the presence of IGF-I in plasma samples.
Popot
Marie-Agnes
MA
LCH, Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370, Verrières le Buisson, France. mariepopot@wanadoo.fr.
Woolfitt
Adrian R
AR
Garcia
Patrice
P
Tabet
Jean-Claude
JC
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006736:Horses; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D007334:Insulin-Like Growth Factor I