Overview of the retrovirus transduction system.
A retrovirus vector is an infectious virus used to introduce a nonviral gene into mitotic cells in vivo or in vitro. The efficient and precise integration machinery of naturally occurring retroviruses is utilized to produce either a single copy or a few copies of the viral genome stably integrated into a host chromosome. This unit presents an overview of the retrovirus life cycle and a description of vector designs and packaging cell lines.
Cepko
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Harvard Medical School, Boston, Massachusetts, USA.
Pear
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D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D018014:Gene Transfer Techniques; D005814:Genes, Viral; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D008018:Life Cycle Stages; D051379:Mice; D010957:Plasmids; D011784:Quail; D012367:RNA, Viral / Q000235:genetics; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development; D014161:Transduction, Genetic / Q000379:methods*; D014771:Virion / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral