title: Differential display of mRNA by PCR.
abstract: Formerly UNIT , this unit describes how differential display techniques allow the identification and subsequent isolation of differentially expressed genes that requires no knowledge of sequences, but rather PCR amplification using arbitrary oligonucleotides and high resolution polyacrylamide gel electrophoresis.
authors.affiliation: Vanderbilt-Ingram Cancer Center, Nashville, Tennessee, USA.
authors.forename: P
authors.initials: P
authors.lastname: Liang
authors.forename: A B
authors.initials: AB
authors.lastname: Pardee
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger / Q000235:genetics*; D048348:Reverse Transcription
chemical_list: D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); D004247:DNA