title: Distribution of L-azetidine-2-carboxylate N-acetyltransferase in yeast.
abstract: The budding yeast Saccharomyces cerevisiae Sigma1278b contains the MPR1 gene encoding N-acetyltransferase, which detoxifies the L-proline analog L-azetidine-2-carboxylate (AZC). Of 131 yeasts tested, AZC acetyltransferase activity was detected in 17 strains of 41 strains that showed AZC resistance. Degenerate-PCR analysis revealed that two strains, i.e., Candida saitoana AKU4533 and Wickerhamia fluorescens AKU4722, contained a DNA fragment highly homologous to MPR1. This indicates that AZC acetyltransferases are widely distributed in yeasts.
authors.affiliation: Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. wada@chem.agr.hokudai.ac.jp
authors.forename: Masaru
authors.initials: M
authors.lastname: Wada
authors.forename: Kae
authors.initials: K
authors.lastname: Okabe
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kataoka
authors.forename: Sakayu
authors.initials: S
authors.lastname: Shimizu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yokota
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takagi
mesh_terms: D001383:Azetidinecarboxylic Acid / Q000378:metabolism*; D005800:Genes, Fungal; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D001383:Azetidinecarboxylic Acid