title: Immunization of mice.
abstract: In the protocols in this unit, antigen is prepared for injection either by emulsifying an antigen solution with Freunds adjuvant or by homogenizing a polyacrylamide gel slice containing the protein antigen. Mice are immunized at 2- to 3-week intervals. Test bleeds are collected 7 days after each booster immunization to monitor serum antibody levels. Mice are chosen for hybridoma fusions when a sufficient antibody titer is reached.
authors.affiliation: ADI Diagnostics, Rexdale, Ontario.
authors.forename: S A
authors.initials: SA
authors.lastname: Fuller
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: J G
authors.initials: JG
authors.lastname: Hurrell
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood*; D007114:Immunization / Q000379:methods*; D051379:Mice
chemical_list: D000906:Antibodies