title: Direct chemical analysis of glycoconjugates for carbohydrates.
abstract: This unit presents protocols for detection of different sugars bound to glycoconjugates. The solution containing the carbohydrate material is treated with a specific reagent, generating a colored reaction product that can be detected spectrophotometrically. For each assay, the absorbance of the colored solution is proportional to the amount of sugar present in the glycoconjugate.
authors.affiliation: University of California San Diego, La Jolla, California, USA.
authors.forename: A
authors.initials: A
authors.lastname: Manzi
authors.forename: J
authors.initials: J
authors.lastname: Esko
mesh_terms: D000107:Acetylation; D000818:Animals; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D019800:Phenol / Q000032:analysis / Q000737:chemistry; D012118:Resorcinols / Q000737:chemistry; D013464:Sulfuric Acids / Q000032:analysis / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D005290:Ferric Compounds; D012118:Resorcinols; D013464:Sulfuric Acids; D019800:Phenol; C005282:orcinol; D019158:N-Acetylneuraminic Acid; C033158:sulfuric acid