Sensing single base incorporation with nanopore micromanipulation.
Using single-molecule techniques, scientists can routinely investigate the action of a single enzyme. The goal of such studies is usually to gain accurate information unachievable in ensemble assays, such as the maximum instantaneous rate of reaction, the existence of pauses or backward steps, etc. In the article discussed here, the authors have increased their experimental sensitivity so that they can detect a single enzymatic cycle. This improvement makes possible the use of a polymerase enzyme to sequence a single DNA molecule.
Croquette
Vincent
V
Laboratoire de Physique Statistique, Ecole Normale Supérieure, CNRS UMR8550, Université P. M. Curie et Paris Diderot, 24 rue Lhomond, 75005 Paris, France. Vincent.Croquette@lps.ens.fr
D001427:Bacterial Toxins / Q000737:chemistry*; D002384:Catalysis; D017931:DNA Primers / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000096:biosynthesis* / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D006460:Hemolysin Proteins / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D009711:Nucleotides / Q000737:chemistry*; D013208:Staphylococcal Toxoid / Q000737:chemistry
D001427:Bacterial Toxins; D017931:DNA Primers; D004277:DNA, Single-Stranded; D006460:Hemolysin Proteins; D008051:Lipid Bilayers; D009711:Nucleotides; D013208:Staphylococcal Toxoid; C021091:staphylococcal alpha-toxin; D004259:DNA-Directed DNA Polymerase