Overview of the vaccinia virus expression system.
The vaccinia virus expression system differs from others in that transcription occurs in the cytoplasm of mammalian cells rather than in the nucleus. As a vector, vaccinia virus has a number of useful characteristics, including a capacity that permits cloning large fragments of foreign DNA (20+ kbp) with retention of infectivity, a wide host range, a relatively high level of protein synthesis, and "appropriate" transport, secretion, processing, and posttranslational modifications as dictated by the primary structure of the expressed protein and the cell type used. This overview discusses the life cycle of the vaccinia virus along with effects of vaccinia infection. The vaccinia vector expression system is described along with specific steps for expressing genes using these vectors. Important safety considerations are also presented.
Moss
Bernard
B
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
Earl
Patricia L
PL
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008322:Mammals; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D017751:Safety Management / Q000379:methods; D014161:Transduction, Genetic / Q000379:methods; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication
D011993:Recombinant Fusion Proteins