Proteomic analysis of methamphetamine-induced reinforcement processes within the mesolimbic dopamine system.
ABSTRACT Methamphetamine (MAP) is a commonly used, addictive drug, and a powerful stimulant that dramatically affects the central nervous system. In this study, we used the conditioned place preference (CPP) paradigm in order to study the reinforcing properties of MAP and the herewith associated changes in proteins within the mesolimbic dopamine system. A CPP was induced by MAP after three intermittent intraperitoneal injections (1 mg/kg) in rats and protein profiles in the nucleus accumbens, striatum, prefrontal cortex, cingulate cortex and hippocampus were compared with a saline-treated control group. In addition, a group of animals was run through extinction and protein profiles were compared with a non-extinguished group. Protein screening was conducted using two-dimensional electrophoresis analysis which identified 27 proteins in the group that showed MAP-induced CPP. Some of the proteins were confirmed by Western lot analysis. Identified proteins had functions related to the cytoskeleton, transport/endocytosis or exocytosis (e.g. profilin-2 and syntaxin-binding protein), and signal transduction, among others.
Yang
Moon Hee
MH
Department of Biological Science, Sookmyung Women's University, Korea.
Kim
Seyoon
S
Jung
Min-Suk
MS
Shim
Jung Hee
JH
Ryu
Na Kyung
NK
Yook
Yeon Joo
YJ
Jang
Choon-Gon
CG
Bahk
Young Yil
YY
Kim
Kee-Won
KW
Park
Jong Hoon
JH
D000818:Animals; D000697:Central Nervous System Stimulants / Q000008:administration & dosage / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008032:Limbic System / Q000187:drug effects*; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage / Q000494:pharmacology*; D009473:Neuronal Plasticity / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012054:Reinforcement, Psychology
D000697:Central Nervous System Stimulants; D008694:Methamphetamine; D004298:Dopamine