title: A/G Gln20Arg (exon 1) and G/A Val156Met (exon 5) polymorphisms of the human orosomucoid 1 gene in Mexico.
abstract: The human orosomucoid 1 gene (ORM1) codes an alpha-1-acid glycoprotein that has been classified as an acute-phase reactive protein, and a major drug-binding serum component, as well as an immunomodulatory protein with genetic polymorphisms. Evaluation of ORM variation through isoelectric focusing and immunobloting has revealed a world-wide distribution of the ORM1 F and ORM1 S alleles. We evaluated and examined the genetic characteristics of two Mexican populations that have different anthropological and cultural antecedents, examining two ORM1 genotypes (exon 1 - A/G (Gln20Arg) and exon 5 G/A (Val156Met)) in 145 individuals, using nested polymerase chain reaction, sequencing, and restricted fragment length polymorphism. Mexican Mestizos had higher frequencies of the exon 1 A allele (P = 0.020) and AA genotype (P = 0.018) and lower frequency of the G allele (P = 0.020) when compared to Teenek Amerindians. When we examined exon 5 G/A (Val156Met) polymorphisms, we found significantly higher frequencies of the G allele (P = 0.0007) and the GG genotype (P = 0.0003) in the Mexican Mestizo population. The Teenek population had a significantly higher frequency of the A allele than has been reported for Chinese and African (P < 0.05) populations, and the G/A genotype was more frequently found in this Mexican population than in Chinese, African and European populations (P < 0.05).
authors.affiliation: Research Unit, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico. garortiz@yahoo.com
authors.forename: L
authors.initials: L
authors.lastname: García-Ortiz
authors.forename: G
authors.initials: G
authors.lastname: Vargas-Alarcón
authors.forename: J M
authors.initials: JM
authors.lastname: Fragoso
authors.forename: J
authors.initials: J
authors.lastname: Granados
authors.forename: L
authors.initials: L
authors.lastname: Maldonado Noriega
authors.forename: A
authors.initials: A
authors.lastname: Navas Pérez
authors.forename: E
authors.initials: E
authors.lastname: Huerta Reyes
authors.forename: J C
authors.initials: JC
authors.lastname: Zenteno-Ruiz
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Cordero
mesh_terms: D000483:Alleles; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons / Q000235:genetics*; D005787:Gene Frequency; D014644:Genetic Variation; D005828:Genetics, Population; D006801:Humans; D007198:Indians, North American / Q000235:genetics*; D008800:Mexico; D009961:Orosomucoid / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013223:Statistics as Topic
chemical_list: D009961:Orosomucoid; D004247:DNA