Synthesis and purification of oligonucleotides.
This unit provides strategies on the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection of DNA and RNA oligonucleotides.
Ellington
A
A
University of Texas, Austin, Texas, USA.
Pollard
J D
JD
D009711:Nucleotides / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D014320:Triphenylmethyl Compounds / Q000737:chemistry
D009711:Nucleotides; D009841:Oligonucleotides; D014320:Triphenylmethyl Compounds