[Impact of up-regulating Ezrin expression by Epstein-Barr virus latent membrane protein 1 on metastasis ability of nasopharyngeal carcinoma cells].
OBJECTIVE
Ezrin, a cytoskeleton-related protein, contributes to the invasion and metastasis of tumors; our previous study had also shown that Epstein-Barr virus latent membrane protein 1 (EBV-LMP1) can promote the metastasis of nasopharyngeal carcinoma (NPC) cells. This study was to investigate whether EBV-LMP1 could change the expression of Ezrin and the role of Ezrin on the metastasis of NPC cells.


METHODS
The expression of EBV-LMP1 and Ezrin in NPC cell lines CNE1 (well differentiated NPC cell line) and CNE1-GL (CNE1 cells stably transfected with EBV-LMP1) were detected by SP immunohistochemistry and Western blot. The adhesive abilities of CNE1, CNE1-GL and AntiEzrin-CNE1-GL (CNE1-GL cells pretreated with Ezrin antibody) cells were assessed by cell-matrix adhesion assay. The invasion and migration of these cell lines were investigated by Transwell method.


RESULTS
Ezrin was not expressed in CNE1 cells while strongly expressed in CNE1-GL cells. The adhesive ability of CNE1-GL cells with matrix was significantly higher than those of CNE1 and AntiEzrin-CNE1-GL cells [adhesive rate: (89.38+/-6.12)% vs. (49.42+/-5.37)% and (56.94+/-4.08)%, P<0.001, P<0.05]. The invasion and migration of CNE1-GL cells were significantly higher than those of CNE1 and AntiEzrin-CNE1-GL cells (invasive cells: 107+/-11 vs. 27+/-3 and 38+/-4, P<0.001; migratory cells: 179+/-25 vs. 46+/-6 and 51+/-5, P<0.001).


CONCLUSIONS
EBV-LMP1 contributes to the metastasis ability of CNE1 cells by up-regulating the expression of Ezrin.
Shen
Zhi-Hua
ZH
Department of Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong, 524023, P. R. China.
Chen
Xiao-Yi
XY
Chen
Jin
J
D045744:Cell Line, Tumor; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000235:genetics*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D015854:Up-Regulation; D014763:Viral Matrix Proteins / Q000502:physiology*; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D014763:Viral Matrix Proteins; D000099149:Ezrin; C037662:EBV-associated membrane antigen, Epstein-Barr virus