title: Osmotic pressure and aggregate shape in BSA/poly(ethylene glycol)-lipid/Dextran solutions.
abstract: In this work we study the colloidal osmotic pressure (COP) and aggregate shape in phosphate saline buffer solutions (pH 7.4) containing bovine serum albumin (BSA), poly(ethylene glycol) lipid (PEG(2000)-PE) and Dextran (Dx). Dx was added to the BSA/PEG(2000)-PE system in order to increase the COP of the solution to levels comparable to the COP of healthy adults, with the aim of using the solution as a blood COP regulator. Dynamic light scattering and small angle X-ray scattering results shown the formation of BSA/PEG(2000)-PE/Dx aggregates in the solution. Osmometry results shown that the addition of Dx to the BSA/PEG(2000)-PE system could successfully increase the COP, through the formation of BSA/PEG(2000)-PE/Dx aggregates. The BSA/PEG(2000)-PE/Dx solutions attained COP=15 mm Hg, representing 60% of COP measured for healthy adults.
authors.affiliation: School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, P.O. Box 226, Whiteknights, Reading, UK. V.Castelletto@reading.ac.uk
authors.forename: Valeria
authors.initials: V
authors.lastname: Castelletto
authors.forename: Ian W
authors.initials: IW
authors.lastname: Hamley
authors.forename: Luke A
authors.initials: LA
authors.lastname: Clifton
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Green
mesh_terms: D000818:Animals; D002417:Cattle; D003102:Colloids; D003911:Dextrans / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D009997:Osmotic Pressure; D011092:Polyethylene Glycols / Q000737:chemistry*; D053838:Scattering, Small Angle; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D014961:X-Ray Diffraction
chemical_list: D003102:Colloids; D003911:Dextrans; D008055:Lipids; D012996:Solutions; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols