title: Guanidine methods for total RNA preparation.
abstract: Three different methods for RNA preparation using guanidine are presented in this unit--a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Kingston
authors.forename: P
authors.initials: P
authors.lastname: Chomczynski
authors.forename: N
authors.initials: N
authors.lastname: Sacchi
mesh_terms: D000818:Animals; D002122:Calcium Chloride; D002478:Cells, Cultured / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification; D019791:Guanidine; D007202:Indicators and Reagents; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification*; D012996:Solutions
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D012996:Solutions; D012313:RNA; D004247:DNA; D019791:Guanidine; D002122:Calcium Chloride