Identification of RNA binding proteins by UV cross-linking.
One of the major focuses of modern biology is to understand the dynamics of RNA-protein interactions, including factors that interact with mRNA, rRNA, tRNA, snRNA, hnRNA, siRNA, and viral RNA. Identification the direct interactions between proteins and RNA has greatly advanced our knowledge about the function of RNA-protein machines. UV crosslinking is a straightforward and powerful tool in this endeavor.
Luo
Ming-Juan
MJ
Harvard Medical School, Boston, Massachusetts, USA.
Reed
Robin
R
D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D010761:Phosphorus Radioisotopes / Q000032:analysis; D011485:Protein Binding; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism / Q000528:radiation effects; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D017381:Spliceosomes / Q000737:chemistry / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D016601:RNA-Binding Proteins