[Fluorometric determination of fructose in the presence of glucose using zirconyl chloride-ammonium chloride].
According to the principle that Zn(OH)+ produced by ZrOCl2 upon hydrolysis in water can react to form a fluorescent derivative with fructose, a method for the quantitative determination of fructose has been proposed. The fluorescence of the derivative enhanced at 408 nm in NH4Cl medium has a direct ratio with fructose in the linear range of 0.5-25 microg x mL(-1). When reaction conditions such as a temperature of 70 degrees C and a time of 15 min are used, the detection limits is 0.071 mg x mL(-1). The proposed method was also applied successfully to the determination of fruit, satisfaction with high accuracy and good reproduction.
Tong
Lei
L
School of Environmental Studies and Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education, China University of Geosciences, Wuhan 430074, China. tonglei0710@163.com
Wang
Yan-Xin
YX
Zhao
Zhong-Yi
ZY
Jin
Ji-Hong
JH
Guo
Xiao-Hui
XH
D000643:Ammonium Chloride / Q000737:chemistry; D005632:Fructose / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000643:Ammonium Chloride; D005632:Fructose; D005947:Glucose