title: Crystals of DhaA mutants from Rhodococcus rhodochrous NCIMB 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis.
abstract: The enzyme DhaA from Rhodococcus rhodochrous NCIMB 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. The haloalkane dehalogenase DhaA and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (TCP). Three mutants named DhaA04, DhaA14 and DhaA15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. All protein mutants were crystallized using the sitting-drop vapour-diffusion method. The crystals of DhaA04 belonged to the orthorhombic space group P2(1)2(1)2(1), while the crystals of the other two mutants DhaA14 and DhaA15 belonged to the triclinic space group P1. Native data sets were collected for the DhaA04, DhaA14 and DhaA15 mutants at beamline X11 of EMBL, DESY, Hamburg to the high resolutions of 1.30, 0.95 and 1.15 A, respectively.
authors.affiliation: Institute of Physical Institute of Physical Biology, University of South Bohemia Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Stsiapanava
authors.forename: Tana
authors.initials: T
authors.lastname: Koudelakova
authors.forename: Mikalai
authors.initials: M
authors.lastname: Lapkouski
authors.forename: Martina
authors.initials: M
authors.lastname: Pavlova
authors.forename: Jiri
authors.initials: J
authors.lastname: Damborsky
authors.forename: Ivana Kuta
authors.initials: IK
authors.lastname: Smatanova
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D009154:Mutation; D011487:Protein Conformation; D012240:Rhodococcus / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers