title: SNP-finding in pig mitochondrial ESTs.
abstract: The Sino-Danish pig genome project produced 685 851 ESTs (Gorodkin et al. 2007), of which 41 499 originated from the mitochondrial genome. In this study, the mitochondrial ESTs were assembled, and 374 putative SNPs were found. Chromatograms for the ESTs containing SNPs were manually inspected, and 112 total (52 non-synonymous) SNPs were found to be of high confidence (five of them are close to disease-causing SNPs in humans). Nine of the high-confidence SNPs were tested experimentally, and eight were confirmed. The SNPs can be accessed online at http://pigest.ku.dk/more/mito.
authors.affiliation: Division of Genetics and Bioinformatics, IBHV, University of Copenhagen, Grønnegårdsvej 3, DK-1870 Frederiksberg, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Scheibye-Alsing
authors.forename: S
authors.initials: S
authors.lastname: Cirera
authors.forename: M J
authors.initials: MJ
authors.lastname: Gilchrist
authors.forename: M
authors.initials: M
authors.lastname: Fredholm
authors.forename: J
authors.initials: J
authors.lastname: Gorodkin
mesh_terms: D000818:Animals; D016001:Confidence Intervals; D020224:Expressed Sequence Tags; D005787:Gene Frequency; D016678:Genome; D006801:Humans; D008928:Mitochondria / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013045:Species Specificity; D013552:Swine / Q000235:genetics*