title: AQP1 expression analysis in human diseases: implications for proteomic characterization.
abstract: Aquaporin (AQP)1 belongs to a ubiquitous family of water channel proteins characterized by sequence similarity and the presence of two NPA (Asp-Pro-Ala) motifs existing in almost all organs and tissues. Currently, 13 human AQPs are known and they are divided into two subgroups according to their ability to transport only water molecules, such as AQP1, or also glycerol and other small solutes. The genomic, structural and functional aspects of AQP1 are briefly described. An in-depth discussion is devoted to proteomic approaches that are useful for identifying and characterizing AQP1, mainly through electrophoretic techniques combined with different extraction procedures followed by mass spectrometry analysis. Moreover, the relevance of AQP1 in human diseases is also explained. Its role in human tumors and, in particular, those of the kidney (e.g., clear cell renal carcinoma) is discussed.
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, Via Cadore 48, 20052 Monza, Italy. fulvio.magni@unimib.it
authors.forename: Fulvio
authors.initials: F
authors.lastname: Magni
authors.forename: Clizia
authors.initials: C
authors.lastname: Chinello
authors.forename: Francesca
authors.initials: F
authors.lastname: Raimondo
authors.forename: Paolo
authors.initials: P
authors.lastname: Mocarelli
authors.forename: Marzia Galli
authors.initials: MG
authors.lastname: Kienle
authors.forename: Marina
authors.initials: M
authors.lastname: Pitto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051398:Aquaporin 1 / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D001921:Brain / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods; D006031:Glycosylation; D006801:Humans; D007672:Kidney Cortex / Q000737:chemistry; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology; D007680:Kidney Neoplasms / Q000378:metabolism; D008322:Mammals; D013058:Mass Spectrometry / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis; D009369:Neoplasms / Q000378:metabolism; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D014867:Water / Q000378:metabolism
chemical_list: C496020:AQP1 protein, human; D009363:Neoplasm Proteins; D014867:Water; D051398:Aquaporin 1