Fungal laccase, cellobiose dehydrogenase, and chemical mediators: combined actions for the decolorization of different classes of textile dyes.
Dyes belonging to the mono-, di-, tri- and poly-azo as well as anthraquinonic and mono-azo Cr-complexed classes, chosen among the most utilized in textile applications, were employed for a comparative enzymatic decolorization study using the extracellular crude culture extracts from the white rot fungus Funalia (Trametes) trogii grown on different culture media and activators able to trigger different levels of expression of oxidizing enzymes: laccase and cellobiose dehydrogenase. Laccase containing extracts were capable to decolorize some dyes from all the different classes analyzed, whereas the recalcitrant dyes were subjected to the combined action of laccase and the chemical mediator HBT, or laccase plus cellobiose dehydrogenase. Correlations among the decolorization degree of the various dyes and their electronic and structural diversities were rationalized and discussed. The utilization of cellobiose dehydrogenase in support to the activity of laccase for the decolorization of azo textile dyes resulted in substantial increases in decolorization for all the refractory dyes proving to be a valid alternative to more expensive and less environmentally friendly chemical treatments of textile dyes wastes.
Ciullini
Ilaria
I
Laboratorio di Chimica Bioinorganica, Dipartimento di Chimica, Università degli Studi di Firenze, Via Della Lastruccia 3, 50019 Firenze, Italy.
Tilli
Silvia
S
Scozzafava
Andrea
A
Briganti
Fabrizio
F
D001487:Basidiomycota / Q000201:enzymology*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D013783:Textile Industry
D004396:Coloring Agents; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D042845:Laccase