title: DNA-independent RNA polymerases.
abstract: Poly(A) polymerase, purified from E. coli, catalyzes the incorporation of AMP residues onto the free 3'-hydroxyl terminus of RNA, utilizing ATP as a precursor. This unit describes specific reaction conditions as well as some applications including labeling the 3' ends of RNA with alpha32ATP for hybridization probes, and cloning RNA that lacks a poly(A) tail. The poly(A) tail is synthesized by poly(A) polymerase, and cDNA is synthesized from the resulting RNA using oligo(dT) as a primer.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: S
authors.initials: S
authors.lastname: Tabor
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D009693:Nucleic Acid Hybridization
chemical_list: D012321:DNA-Directed RNA Polymerases