title: Gene silencing by RNAi in mammalian cells.
abstract: This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways for siRNA generation and optimisation, as well as recommendations for cell transfection are presented.
authors.affiliation: Ribopharma AG, Kulmbach, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: John
authors.forename: Anke
authors.initials: A
authors.lastname: Geick
authors.forename: Philipp
authors.initials: P
authors.lastname: Hadwiger
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Vornlocher
authors.forename: Olaf
authors.initials: O
authors.lastname: Heidenreich
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D017930:Genes, Reporter; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D008081:Liposomes; D008322:Mammals / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics*; D014162:Transfection / Q000379:methods*
chemical_list: D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D034741:RNA, Small Interfering