A promoter sequence variant of ZNF750 is linked with familial psoriasis.
We previously mapped a psoriasis-susceptibility gene to a 3.8-Mb region of the 17q terminus in a five-generation Chinese family with autosomal-dominant psoriasis. To identify the mutations responsible for the psoriasis in this family, we sequenced 78 genes within the region and found four gene variants, p.Ala201Val in CD7, c.-625A>C in zinc-finger protein 750 (ZNF750), p.Asp189Asn in C17orf56, and p.Ala568Thr in AATK cosegregated with the disease. The latter two variants were not studied further in the absence of disease segregation in other familial psoriasis and presence of variants in normal subjects. Functional analyses of CD7 did not support CD7 as a disease-causing gene. In contrast, the c.-625A>C mutation in ZNF750 resulted in a 42% reduction of the promoter activity, and the electrophoretic mobility shift assay showed binding of nuclear protein(s) to the mutant C allele. The c.-625A>C mutation was found in another sporadic psoriasis patient but was absent in 188 normal controls. Together, the mutation accounts for 1.7% (confidence interval: 0.2-5.84%) of psoriasis in the Chinese population. This report suggests that ZNF750 mutations could contribute to psoriasis susceptibility.
Yang
Chi-Fan
CF
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Hwu
Wuh-Liang
WL
Yang
Li-Cheng
LC
Chung
Wen-Hung
WH
Chien
Yin-Hsiu
YH
Hung
Chia-Fu
CF
Chen
Hung-Chih
HC
Tsai
Pei-Joung
PJ
Fann
Cathy S J
CS
Liao
Fang
F
Chen
Yuan-Tsong
YT
D019011:Antigens, CD7 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17; D020022:Genetic Predisposition to Disease; D006801:Humans; D009154:Mutation; D011401:Promoter Regions, Genetic; D011565:Psoriasis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D016335:Zinc Fingers / Q000235:genetics*
D019011:Antigens, CD7; D012333:RNA, Messenger