title: Depressed and anxious mood and T-cell cytokine expressing populations in ovarian cancer patients.
abstract: The adaptive immune response of ovarian cancer patients has been linked to survival, and is known to be impaired in the tumor microenvironment. Little is known about relationships between biobehavioral factors such as depressed mood and anxiety and the adaptive immune response in ovarian cancer. Thirty-seven patients with epithelial ovarian cancer and 14 patients with benign ovarian neoplasms completed psychosocial questionnaires pre-surgery. Lymphocytes from peripheral blood, tumor, and ascites (fluid around the tumor), were obtained on the day of surgery. Expression of the Type-1 cytokine interferon-gamma (IFN gamma), and the Type-2 cytokine interleukin-4 (IL-4) by T-helper (CD4(+)) and T-cytotoxic (CD8(+)) cells was measured under autologous tumor-stimulated, polyclonally-stimulated, or unstimulated conditions. Links with mood were examined. Among cancer patients, marked elevations in unstimulated and tumor-stimulated Type-2 responses were seen, particularly in ascites and tumor-infiltrating lymphocytes (P values<0.01). With polyclonal stimulation, lymphocytes from all compartments expressed elevated Type-1 cytokines (P values<0.014). Depressed and anxious mood were both associated with significantly lower ratios of polyclonally-stimulated CD4(+) cells producing IFN gamma (TH(1) cells) vs. IL-4 (TH(2) cells) in all compartments (depressed mood: P=0.012; anxiety: P=0.038) and depressed mood was also related to lower ratios of polyclonally-stimulated CD8(+) cells producing IFN gamma (TC(1)) vs. IL-4 (TC(2)) (P=0.035). Although effects of polyclonal stimulation should be generalized with caution to the in vivo immune response, findings suggest that depressed and anxious mood are associated with greater impairment of adaptive immunity in peripheral blood and in the tumor microenvironment among ovarian cancer patients.
authors.affiliation: Department of Psychology, E11 Seashore Hall, University of Iowa, Iowa City, IA 52242, USA. susan-lutgendorf@uiowa.edu
authors.forename: Susan K
authors.initials: SK
authors.lastname: Lutgendorf
authors.forename: Donald M
authors.initials: DM
authors.lastname: Lamkin
authors.forename: Koen
authors.initials: K
authors.lastname: DeGeest
authors.forename: Barrie
authors.initials: B
authors.lastname: Anderson
authors.forename: Minh
authors.initials: M
authors.lastname: Dao
authors.forename: Stephanie
authors.initials: S
authors.lastname: McGinn
authors.forename: Bridget
authors.initials: B
authors.lastname: Zimmerman
authors.forename: Heena
authors.initials: H
authors.lastname: Maiseri
authors.forename: Anil K
authors.initials: AK
authors.lastname: Sood
authors.forename: David M
authors.initials: DM
authors.lastname: Lubaroff
mesh_terms: D000368:Aged; D001007:Anxiety / Q000209:etiology / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism*; D003863:Depression / Q000209:etiology / Q000276:immunology*; D005260:Female; D006801:Humans; D007109:Immunity / Q000502:physiology; D007370:Interferon Type I / Q000096:biosynthesis / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000150:complications / Q000276:immunology* / Q000523:psychology*; D011795:Surveys and Questionnaires; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000378:metabolism
chemical_list: D016207:Cytokines; D007370:Interferon Type I; D015847:Interleukin-4