Generation of a conditional null allele for Dmp1 in mouse.
Dentin matrix protein1 (DMP1), highly conserved in humans and mice, is highly expressed in teeth, the skeleton, and to a lesser extent in nonskeletal tissues such as brain, kidney, and salivary gland. Pathologically, DMP1 is associated with several forms of cancers and with tumor-induced osteomalacia. Conventional disruption of the murine Dmp1 gene results in defects in dentin in teeth and in the skeleton, including hypophosphatemic rickets, and abnormalities in phosphate homeostasis. Human DMP1 mutations are responsible for the condition known as autosomal recessive hypophosphatemic rickets. For better understanding of the roles of DMP1 in different tissues at different stages of development and in pathological conditions, we generated Dmp1 floxed mice in which loxP sites flank exon 6 that encodes for over 80% of DMP1 protein. We demonstrate that Cre-mediated recombination using Sox2-Cre, a Cre line expressed in epiblast during early embryogenesis, results in early deletion of the gene and protein. These homozygous Cre-recombined null mice display an identical phenotype to conventional null mice. This animal model will be useful to reveal distinct roles of DMP1 in different tissues at different ages.
Feng
Jian Q
JQ
Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, Texas 75246, USA. jfeng@bcd.tamhsc.edu
Scott
Greg
G
Guo
Dayong
D
Jiang
Baichun
B
Harris
Marie
M
Ward
Toni
T
Ray
Manas
M
Bonewald
Lynda F
LF
Harris
Stephen E
SE
Mishina
Yuji
Y
D000818:Animals; D001842:Bone and Bones / Q000196:embryology* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005091:Exons; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D017353:Gene Deletion; D005821:Genetic Techniques; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic
C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; C045073:Cre recombinase; D019426:Integrases