A novel approach obtaining intron-containing hairpin RNA constructs.
Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target genes efficiently in a variety of species. Here we designed a new recombinant-PCR mediated method called direct amplification of ihpRNA from genomic DNA. This approach has proved to be easy, stable, and efficient.
Wen
Chun-Miao
CM
Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066, PR China.
Cai
Ping-Zhong
PZ
Xu
Zheng-Jun
ZJ
Zhong
Wan-Fang
WF
D001483:Base Sequence; D020868:Gene Silencing; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*
D012313:RNA