Discovering mechanisms of signaling-mediated cysteine oxidation.
Accumulating evidence reveals hydrogen peroxide as a key player both as a damaging agent and, from emerging evidence over the past decade, as a second messenger in intracellular signaling. This rather mild oxidant acts upon downstream targets within signaling cascades to modulate the activity of a host of enzymes (e.g. phosphatases and kinases) and transcriptional regulators through chemoselective oxidation of cysteine residues. With the recent development of specific detection reagents for hydrogen peroxide and new chemical tools to detect the generation of the initial oxidation product, sulfenic acid, on reactive cysteines within target proteins, the scene is set to gain a better understanding of the mechanisms through which hydrogen peroxide acts as a second messenger in cell signaling.
Poole
Leslie B
LB
Department of Biochemistry, Center for Structural Biology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, United States. lbpoole@wfubmc.edu
Nelson
Kimberly J
KJ
D003545:Cysteine / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction
D006861:Hydrogen Peroxide; D054464:Peroxiredoxins; D003545:Cysteine