title: Sin Nombre virus shedding patterns in naturally infected deer mice (Peromyscus maniculatus) in relation to duration of infection.
abstract: A 2-year capture-mark-recapture study was conducted in southern Manitoba, Canada, to test for an association between the duration of Sin Nombre virus (SNV) infection in deer mice (Peromyscus maniculatus) and virus shedding. Hantavirus-specific IgG antibodies were detected in 22.2% of captured deer mice, and recently infected deer mice were identified based on the detection of low-avidity IgG antibodies. SNV RNA was detected in blood samples from the majority of seropositive deer mice with no significant difference in the association of SNV RNA between the low- and high-avidity groups (57.8% and 52.1%, respectively). A small subset of seropositive mice (11.6%) had detectable SNV RNA in oropharyngeal fluids (OPF) or urine. A greater proportion of deer mice with low-avidity antibodies had SNV RNA in OPF or urine compared with rodents with high-avidity antibodies (21% versus 6.8%, respectively). This is the first study of naturally infected deer mice to provide evidence that recently infected mice are more likely to shed SNV and thus might represent a greater risk of human infection.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Safronetz
authors.forename: Michael A
authors.initials: MA
authors.lastname: Drebot
authors.forename: Harvey
authors.initials: H
authors.lastname: Artsob
authors.forename: Tyler
authors.initials: T
authors.lastname: Cote
authors.forename: Kai
authors.initials: K
authors.lastname: Makowski
authors.forename: L Robbin
authors.initials: LR
authors.lastname: Lindsay
mesh_terms: D000818:Animals; D000915:Antibody Affinity / Q000276:immunology*; D005260:Female; D018804:Hantavirus Pulmonary Syndrome / Q000635:transmission* / Q000662:veterinary; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008350:Manitoba / Q000453:epidemiology; D010542:Peromyscus / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification; D029261:Sin Nombre virus / Q000302:isolation & purification*; D017201:Virus Shedding
chemical_list: D007074:Immunoglobulin G; D012367:RNA, Viral