[Cloning and sequence analysis of SOCS-2 gene in pig].
Total RNA was isolated from kidney of BaMei pig, a local strain of Chinese pig, and then the cDNA sequence of SOCS-2 gene was cloned by RT-PCR (GenBank accepted number is EF121242). Then the cloned SOCS-2 gene was inserted into PMD19-T vector by T/A cloning, transformed into DH-5alpha, tested by PCR and sequenced. The data show that the homology of the cloned porcine SOCS-2, including 822 bp, is more than 93% and that of the deduced amino acid sequence is 89% when compared with human, rat and mice. And the molecular weight of SOCS-2 protein is about 22.25 kD and PI is 8.03. The cloning of SOCS-2 gene is useful for the further research on the molecular mechanism by which regulating growth and development of organism.
Du
Bao-Wen
BW
Northwest A&F University, Laboratory of Animal Fat Deposition and Muscle Development, Yang Ling 712100, China.
Yang
Gong-She
GS
Sun
Chao
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis; D017385:Sequence Homology; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D018076:DNA, Complementary; D050826:Suppressor of Cytokine Signaling Proteins