[Optimization of cell growth and 20-hydroxyecdysone production in cell suspension culture of Vitex glabrata R. Br].
The effects of the cultivation media, plant growth regulators and inoculum size on the cell growth and 20-hydroxyecdysone production in suspension cultures of Vitex glabrata R. Br. were investigated. The cell growth and 20-hydroxyecdysone formation reach the highest when cells are cultured in the Gamborg's B5 medium supplemented with 2.0 mg/L BAP (6-benzylaminopurine) and 1.0 mg/L 2,4-D. The maximum 20-hydroxyecdysone productivity, of about 1.l mg/L/day, was observed in the culture with 20% PCV (packed cell volume) of inoculum size. These data also show that the increment of the inoculum size to 20% PCV could increase the productivity in 7-folds.
Sinlaparaya
Duangjai
D
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002 Thailand.
Duanghaklang
Preeyada
P
Panichajakul
Sanha
S
D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D004441:Ecdysterone / Q000096:biosynthesis*; D020950:Vitex / Q000166:cytology* / Q000378:metabolism*
D003470:Culture Media; D004441:Ecdysterone