Osmotic pressure and aggregate shape in BSA/poly(ethylene glycol)-lipid/Dextran solutions.
In this work we study the colloidal osmotic pressure (COP) and aggregate shape in phosphate saline buffer solutions (pH 7.4) containing bovine serum albumin (BSA), poly(ethylene glycol) lipid (PEG(2000)-PE) and Dextran (Dx). Dx was added to the BSA/PEG(2000)-PE system in order to increase the COP of the solution to levels comparable to the COP of healthy adults, with the aim of using the solution as a blood COP regulator. Dynamic light scattering and small angle X-ray scattering results shown the formation of BSA/PEG(2000)-PE/Dx aggregates in the solution. Osmometry results shown that the addition of Dx to the BSA/PEG(2000)-PE system could successfully increase the COP, through the formation of BSA/PEG(2000)-PE/Dx aggregates. The BSA/PEG(2000)-PE/Dx solutions attained COP=15 mm Hg, representing 60% of COP measured for healthy adults.
Castelletto
Valeria
V
School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, P.O. Box 226, Whiteknights, Reading, UK. V.Castelletto@reading.ac.uk
Hamley
Ian W
IW
Clifton
Luke A
LA
Green
Rebecca J
RJ
D000818:Animals; D002417:Cattle; D003102:Colloids; D003911:Dextrans / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D009997:Osmotic Pressure; D011092:Polyethylene Glycols / Q000737:chemistry*; D053838:Scattering, Small Angle; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012996:Solutions; D014961:X-Ray Diffraction
D003102:Colloids; D003911:Dextrans; D008055:Lipids; D012996:Solutions; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols