A highly efficient construction of GM1 epitope tetrasaccharide and its conjugation with KLH.
GM1 epitope tetrasaccharide was synthesized by a condensation of sialyl-alpha(2-3)-gal acceptor and gal-beta(1-3)-GalN donor in a highly efficient manner. After introduction of mercaptohexanol to the tetrasaccharide, it was coupled to maleimide-activated KLH carrier protein to give the desired GM1 epitope-KLH conjugate.
Yoshikawa
Takeru
T
Department of Applied Bio-organic Chemistry, Gifu University, Gifu 501-1193, Japan.
Kato
Yuki
Y
Yuki
Nobuhiro
N
Yabe
Tomio
T
Ishida
Hideharu
H
Kiso
Makoto
M
D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006433:Hemocyanins / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D008070:Lipopolysaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology
D000939:Epitopes; D008070:Lipopolysaccharides; D009844:Oligosaccharides; C023023:lipid-linked oligosaccharides; C037563:G(M1)-oligosaccharide; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin