title: Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions.
abstract: Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.
authors.affiliation: The Rockefeller University, New York, New York 10065, USA.
authors.forename: Lars F
authors.initials: LF
authors.lastname: Westblade
authors.forename: Leonid
authors.initials: L
authors.lastname: Minakhin
authors.forename: Konstantin
authors.initials: K
authors.lastname: Kuznedelov
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Tackett
authors.forename: Emmanuel J
authors.initials: EJ
authors.lastname: Chang
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Mooney
authors.forename: Irina
authors.initials: I
authors.lastname: Vvedenskaya
authors.forename: Qing Jun
authors.initials: QJ
authors.lastname: Wang
authors.forename: David
authors.initials: D
authors.lastname: Fenyö
authors.forename: Michael P
authors.initials: MP
authors.lastname: Rout
authors.forename: Robert
authors.initials: R
authors.lastname: Landick
authors.forename: Brian T
authors.initials: BT
authors.lastname: Chait
authors.forename: Konstantin
authors.initials: K
authors.lastname: Severinov
authors.forename: Seth A
authors.initials: SA
authors.lastname: Darst
mesh_terms: D000595:Amino Acid Sequence; D017122:Bacteriophage T4 / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000821:virology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012321:DNA-Directed RNA Polymerases