title: Growth and manipulation of yeast.
abstract: Preparation of sterile media of consistently high quality is essential for the genetic manipulation of yeast. Recipes for media needed in the protocols in this chapter are provided in this unit. Specific suppliers are recommended for specific ingredients.
authors.affiliation: Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Treco
authors.forename: F
authors.initials: F
authors.lastname: Winston
mesh_terms: D003470:Culture Media; D004171:Diploidy; D049770:Genes, Mating Type, Fungal; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013172:Spores, Fungal / Q000502:physiology
chemical_list: D003470:Culture Media