Mouse colony management.
The management of mouse colonies created by gene targeting is described in this unit. It defines guidelines necessary to establish the colony, and describes screening for germline transmission, marking the mice for easy and consistent identification, and genotyping DNA by PCR or Southern blotting analysis. It also reviews the software currently available for colony data management.
Conner
David A
DA
Harvard Medical School, Cambridge, Massachusetts, USA.
D000822:Animal Husbandry / Q000379:methods*; D000823:Animal Identification Systems / Q000295:instrumentation / Q000379:methods; D000818:Animals; D015139:Blotting, Southern / Q000379:methods; D001947:Breeding / Q000379:methods; D004247:DNA / Q000032:analysis / Q000235:genetics; D053595:Embryonic Stem Cells; D005260:Female; D005568:Forms and Records Control; D005838:Genotype; D018095:Germ-Line Mutation; D006200:Hair Color / Q000235:genetics; D006799:Housing, Animal / Q000592:standards; D008297:Male; D051379:Mice / Q000145:classification / Q000235:genetics*; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D012984:Software; D013047:Specific Pathogen-Free Organisms
D004247:DNA