Altered blood-brain barrier integrity in adult aquaporin-4 knockout mice.
To investigate the role of astroglial water channel aquaporin-4 (AQP4) in maintaining blood-brain barrier integrity, structure and permeability of the brain microvessels were investigated in adult AQP4 knockout mice. Altered ultrastructure of brain microvessels, including open tight junctions and swollen perivascular astrocytic endfeet, were frequently observed in the AQP4 null mice. Likewise, AQP4 deficiency significantly downregulated expression of glial fibrillary acidic protein in perivascular processes of astrocytes. Furthermore, the horseradish peroxidase analysis demonstrated hyperpermeability of the blood-brain barrier in AQP4 knockout mice. These findings provide direct evidence that AQP4 is essential for the maintenance of blood-brain barrier integrity.
Zhou
Jianping
J
Department of Anatomy, Histology and Pharmacology of Nanjing Medical University, Jiangsu Province Key Laboratory of Neurodegeneration, Nanjing, Jiangsu, PR China.
Kong
Hui
H
Hua
Xiangdong
X
Xiao
Ming
M
Ding
Jiong
J
Hu
Gang
G
D000818:Animals; D051401:Aquaporin 4 / Q000172:deficiency*; D001808:Blood Vessels / Q000378:metabolism / Q000648:ultrastructure; D001812:Blood-Brain Barrier / Q000502:physiology* / Q000648:ultrastructure; D001929:Brain Edema / Q000235:genetics; D002199:Capillary Permeability / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000493:pharmacokinetics; D051379:Mice; D018345:Mice, Knockout
D051401:Aquaporin 4; D005904:Glial Fibrillary Acidic Protein; D006735:Horseradish Peroxidase