Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy.
This study explored the potential of Raman spectroscopy for the analysis of poly(3-hydroxybutyrate) (PHB) in bacteria. PHB can be formed in large amounts by certain bacteria as a storage material and is of high importance for industrial biodegradable plastic production. Raman spectra were collected from Cupriavidus necator DSM 428 (H16), from its non-PHB-producing mutant strain C. necator DSM 541, and from pure PHB, in order to determine at which Raman shifts a contribution of PHB in bacterial spectra can be expected. The Raman band intensity at ca. 1734 cm(-1) appeared to be suitable for the monitoring of PHB production and consumption. These intensities were linearly related to the PHB concentration (mg L(-1) culture) determined by parallel HPLC analysis. Therefore, Raman spectroscopy is considered as a fast and noninvasive technique for the determination and monitoring of the PHB content in bacteria.
Gelder
Joke De
JD
Department of Analytical Chemistry, Ghent University, Proeftuinstraat 86, 9000 Ghent, Belgium. Raman@UGhent.be
Willemse-Erix
Diana
D
Scholtes
Maarten J
MJ
Sanchez
Jorge I
JI
Maquelin
Kees
K
Vandenabeele
Peter
P
Boever
Patrick De
PD
Puppels
Gerwin J
GJ
Moens
Luc
L
Vos
Paul De
PD