Molecular cloning of PCR products.
It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.
Finney
M
M
MJ Research, Watertown, Massachusetts, USA.
Nisson
P E
PE
Rashtchian
A
A
D020029:Base Pairing; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005822:Genetic Vectors / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D051981:Uracil-DNA Glycosidase
D004247:DNA; D051981:Uracil-DNA Glycosidase