title: Trichostatin A (TSA) improves the development of rabbit-rabbit intraspecies cloned embryos, but not rabbit-human interspecies cloned embryos.
abstract: The interspecies somatic cell nuclear transfer (iSCNT) technique for therapeutic cloning gives great promise for treatment of many human diseases. However, the incomplete nuclear reprogramming and the low blastocyst rate of iSCNT are still big problems. Herein, we observed the effect of TSA on the development of rabbit-rabbit intraspecies and rabbit-human interspecies cloned embryos. After treatment with TSA for 6 hr during activation, we found that the blastocyst rate of rabbit-rabbit cloned embryos was more than two times higher than that of untreated embryos; however, the blastocyst rate of TSA-treated rabbit-human interspecies cloned embryos decreased. We also found evident time-dependent histone deacetylation-reacetylation changes in rabbit-rabbit cloned embryos, but not in rabbit-human cloned embryos from fusion to 6 hr after activation. Our results suggest that TSA-treatment does not improve blastocyst development of rabbit-human iSCNT embryos and that abnormal histone deacetylation-reacetylation changes in iSCNT embryos may account for their poor blastocyst development.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Shi
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Miao
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.forename: Jun-Cheng
authors.initials: JC
authors.lastname: Huang
authors.forename: Zi-Li
authors.initials: ZL
authors.lastname: Lei
authors.forename: Ji-Wen
authors.initials: JW
authors.lastname: Yang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Han
authors.forename: Xiang-Fen
authors.initials: XF
authors.lastname: Song
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
mesh_terms: D000107:Acetylation; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D019976:Cloning, Organism; D047108:Embryonic Development; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D011817:Rabbits
chemical_list: D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A