A microfluidic system for dynamic yeast cell imaging.
The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.
Lee
Philip J
PJ
CellASIC Corporation, San Leandro, CA 94577, USA. pjlee@cellasic.com
Helman
Noah C
NC
Lim
Wendell A
WA
Hung
Paul J
PJ
D049452:Green Fluorescent Proteins / Q000378:metabolism; D008565:Membrane Proteins; D044085:Microfluidics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
C490024:FUS1 protein, S cerevisiae; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins