title: Spatial dissection of the cis- and trans-Golgi compartments in the malaria parasite Plasmodium falciparum.
abstract: The Golgi apparatus forms the heart of the secretory pathway in eukaryotic cells where proteins are modified, processed and sorted. The transport of proteins from the endoplasmic reticulum (ER) to the cis-side of the Golgi complex takes place at specialized ER sub-domains known as transitional ER (tER). We used the Plasmodium falciparum orthologue of Sec13p to analyse tER organization. We show that the distribution of PfSec13p is restricted to defined areas of the ER membrane. These foci are juxtaposed to the Golgi apparatus and might represent tER sites. To further analyse cis- to trans-Golgi architecture, we generated a double transfectant parasite line that expresses the Golgi marker Golgi reassembly stacking protein (GRASP) as a green fluorescent protein fusion and the trans-Golgi marker Rab6 as a DsRed fusion protein. Our data demonstrate that Golgi multiplication is closely linked to tER multiplication, and that parasite maturation is accompanied by the spatial separation of the cis- and trans- face of this organelle.
authors.affiliation: Bernhard-Nocht-Institute for Tropical Medicine, Department of Molecular Parasitology, Malaria II, 20359 Hamburg, Germany.
authors.forename: Nicole S
authors.initials: NS
authors.lastname: Struck
authors.forename: Susann
authors.initials: S
authors.lastname: Herrmann
authors.forename: Iris
authors.initials: I
authors.lastname: Schmuck-Barkmann
authors.forename: Suzana
authors.initials: S
authors.lastname: de Souza Dias
authors.forename: Silvia
authors.initials: S
authors.lastname: Haase
authors.forename: Ana L
authors.initials: AL
authors.lastname: Cabrera
authors.forename: Moritz
authors.initials: M
authors.lastname: Treeck
authors.forename: Caroline
authors.initials: C
authors.lastname: Bruns
authors.forename: Christine
authors.initials: C
authors.lastname: Langer
authors.forename: Alan F
authors.initials: AF
authors.lastname: Cowman
authors.forename: Matthias
authors.initials: M
authors.lastname: Marti
authors.forename: Tobias
authors.initials: T
authors.lastname: Spielmann
authors.forename: Tim W
authors.initials: TW
authors.lastname: Gilberger
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins