Collagen-binding peptide interaction with retinal tissue surfaces.
One of the current challenges in treating age-related macular degeneration (AMD) is the surface modification of the retinal Bruch membrane. In this study, the collagen fibers of the inner collagenous zone of the Bruch membrane were identified as type I and type III. Subsequently, the adsorption of a collagen-binding peptide onto the inner collagenous zone surface was investigated. The collagen-binding peptide was able to bind specifically to the collagen fibers while maintaining the biological activity of the N-terminus biotin tag. These results indicate that the collagen-binding peptide may be used as an anchor to immobilize bioactive molecules on the inner collagenous zone surface of the Bruch membrane.
Sistiabudi
Rizaldi
R
Weldon School of Biomedical Engineering, and Department of Chemistry, Indiana, USA.
Ivanisevic
Albena
A
D000327:Adsorption; D000818:Animals; D001667:Binding, Competitive; D016570:Bruch Membrane / Q000737:chemistry* / Q000378:metabolism; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties; D013552:Swine
D010455:Peptides; D003094:Collagen