Expression of EPO receptor in pancreatic cells and its effect on cell apoptosis.
In order to explore the expression of erythropoietin receptor (EPOR) in pancreatic cell line NIT-1 and its effect on cell apoptosis after binding with erythropoietin (EPO), NIT-1 cells were cultured and expanded. The expression of EPOR was detected using electrophoresis. NIT-1 apoptosis was induced by cytokines and their effects on cell apoptosis and cell insulin secretion were assayed after binding of EPO to EPOR. The results showed that EPOR was expressed in NIT-1 cells. Recombinant human EPO (rHuEPO) had no effect on cell apoptosis but significantly inhibited apoptosis induced by cytokines. rHuEPO had no effect on cell insulin secretion but significantly improved insulin secretion inhibited by cytokines. From these findings, it was concluded that EPOR was expressed in NIT-1 cells and EPO could protect NIT-1 cells from apoptosis induced by cytokines.
Shuai
Hongxia
H
Department of Endocrinology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. 61shx@163.com
Zhang
Ji
J
Yu
Yikai
Y
Zhang
Muxun
M
D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D016207:Cytokines / Q000378:metabolism; D004921:Erythropoietin / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D051379:Mice; D010179:Pancreas / Q000378:metabolism*; D017467:Receptors, Erythropoietin / Q000096:biosynthesis* / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry
D016207:Cytokines; D007328:Insulin; D017467:Receptors, Erythropoietin; D011994:Recombinant Proteins; D004921:Erythropoietin