Evaluation of olive oil mill wastewater toxicity on the mitochondrial bioenergetics after treatment with Candida oleophila.
In a previous work the ability of Candida oleophila to use phenolic compounds as sole carbon and energy source at high concentrations without an additional carbon source was reported. C. oleophila grown in bioreactor batch cultures in a diluted and sterilized olive oil mill wastewater (OMW) caused a significant decrease in the total tannins content but no significant alteration was observed in phenolic acid and fatty acid content. Both treated and untreated OMWs were tested to evaluate the capacity in interfering with mitochondrial bioenergetics. Mitochondrial respiration was not affected by treated OMW on the range of used concentrations, contrary to the untreated OMW. Furthermore, mitochondrial membrane potential and respiratory complexes were always significantly less affected by treated OMW in comparison with untreated OMW. However, supplementary treatment should be applied before OMW could be considered non-toxic.
Peixoto
F
F
Chemistry Department, CECAV, University of Trás-os-Montes and Alto Douro, 5001 Vila Real, Portugal. fpeixoto@utad.pt
Martins
F
F
Amaral
C
C
Gomes-Laranjo
J
J
Almeida
J
J
Palmeira
C M
CM
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001673:Biodegradation, Environmental; D002175:Candida / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D005227:Fatty Acids / Q000032:analysis; D005525:Food-Processing Industry; D007220:Industrial Waste; D008099:Liver / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000502:physiology*; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D008933:Mitochondrial Swelling; D000069463:Olive Oil; D010100:Oxygen / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010938:Plant Oils; D051381:Rats; D017208:Rats, Wistar; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013634:Tannins / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*
D005227:Fatty Acids; D007220:Industrial Waste; D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils; D013634:Tannins; D013384:Succinate Cytochrome c Oxidoreductase; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; D010100:Oxygen