title: Principles and application of fluorescence microscopy.
abstract: This overview discusses the principle of fluorescence along with practical discussions of fluorescent molecular probes, filters and filter sets, multiband filters and multi-dye fluorescence, light sources, objective lenses, image resolution and the point-spread function, fluorescence microscopy of living cells, and immunolabeling.
authors.affiliation: University of California Berkeley, Berkeley, California, USA.
authors.forename: D
authors.initials: D
authors.lastname: Coling
authors.forename: B
authors.initials: B
authors.lastname: Kachar
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002470:Cell Survival; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes