title: NHE10, an osteoclast-specific member of the Na+/H+ exchanger family, regulates osteoclast differentiation and survival [corrected].
abstract: Bone homeostasis is tightly regulated by the balanced actions of osteoblasts (OBs) and osteoclasts (OCs). We previously analyzed the gene expression profile of OC differentiation using a cDNA microarray, and identified a novel osteoclastogenic gene candidate, clone OCL-1-E7 [J. Rho, C.R. Altmann, N.D. Socci, L. Merkov, N. Kim, H. So, O. Lee, M. Takami, A.H. Brivanlou, Y. Choi, Gene expression profiling of osteoclast differentiation by combined suppression subtractive hybridization (SSH) and cDNA microarray analysis, DNA Cell Biol. 21 (2002) 541-549]. In this study, we have isolated full-length cDNAs corresponding to this clone from mice and humans to determine the functional roles of this gene in osteoclastogenesis. The full-length cDNA of OCL-1-E7 encodes 12 membrane-spanning domains that are typical of isoforms of the Na(+)/H(+) exchangers (NHEs), indicating that this clone is a novel member of the NHE family (hereafter referred to as NHE10). Here, we show that NHE10 is highly expressed in OCs in response to receptor activator of nuclear factor-kappaB ligand signaling and is required for OC differentiation and survival.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Seoung Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Taesoo
authors.initials: T
authors.lastname: Kim
authors.forename: Eui-Soon
authors.initials: ES
authors.lastname: Park
authors.forename: Sujeong
authors.initials: S
authors.lastname: Yang
authors.forename: Daewon
authors.initials: D
authors.lastname: Jeong
authors.forename: Yongwon
authors.initials: Y
authors.lastname: Choi
authors.forename: Jaerang
authors.initials: J
authors.lastname: Rho
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010010:Osteoclasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*
chemical_list: C513111:Slc9a2 protein, mouse; D017923:Sodium-Hydrogen Exchangers