title: Growth in liquid media.
abstract: The procedure for inoculating overnight (starter) cultures of E. coli from a single colony is described along with considerations for growing larger cultures. Also included are two methods for monitoring cell growth using a spectrophotometer or a hemacytometer.
authors.affiliation: Clark & Elbing LLP, Boston, Massachusetts, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Elbing
authors.forename: Roger
authors.initials: R
authors.lastname: Brent
mesh_terms: D001431:Bacteriological Techniques; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012996:Solutions; D013053:Spectrophotometry / Q000379:methods
chemical_list: D003470:Culture Media; D012996:Solutions