title: Expression and regulation of periostin/OSF-2 gene in rat uterus and human endometrium.
abstract: Periostin/OSF2 is a ligand for alphavbeta3 and alphavbeta5 integrins and activates the Akt/PKB pathway. Recent reports of periostin/OSF2 gene disrupted mice indicate that periostin/OSF-2 plays an important role in implantation. Quantitative RT-PCR revealed that the expression of periostin/OSF-2 mRNA in rat uteri was reduced to approximately 10% at 12 h after 17beta-estradiol (E2) injection, but was not changed after progesterone (P) injection. RT-PCR revealed the expression of periostin/OSF-2 in human endometrium, cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells. In ESCs, the expression of periostin/OSF-2 mRNA was reduced to approximately 50% at 6 h after E2 treatment. The amount of periostin/OSF2 mRNA in human endometrium significantly increased during mid-proliferative and early secretory phases of menstrual cycle, and decreased during late-proliferative, mid-secretory and late secretory phases. The expression of periostin/OSF2 mRNA significantly decreased in ESCs decidualized by treatment with E2 and P for 7 and 11 days. By immunohistochemistry, the expression of periostin/OSF-2 was strongly detected in endometrial stromal cells during early proliferative, mid-proliferative and early secretory phases, and was strongly detected in endometrial epithelial cells during late secretory phase. This study demonstrated that the expression of periostin/OSF-2 is regulated by ovarian steroid hormones in rat uterus and human endometrium.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Hongo, Japan.
authors.forename: Hisahiko
authors.initials: H
authors.lastname: Hiroi
authors.forename: Mikio
authors.initials: M
authors.lastname: Momoeda
authors.forename: Fumiko
authors.initials: F
authors.lastname: Nakazawa
authors.forename: Minako
authors.initials: M
authors.lastname: Koizumi
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsutsumi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Osuga
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yano
authors.forename: Osamu
authors.initials: O
authors.lastname: Tsutsumi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008597:Menstrual Cycle / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism; D013997:Time Factors; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D012333:RNA, Messenger; D011374:Progesterone