RNAi in transgenic plants.
RNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus-vectors. This unit provides Basic Protocols for transgene and virus induced silencing. It also includes a Basic Protocol for protein overexpression using viral proteins that suppress silencing.
Yin
Yanhai
Y
Iowa State University, Ames, Iowa, USA.
Chory
Joanne
J
Baulcombe
David
D
D035843:Biomedical Research / Q000379:methods*; D020868:Gene Silencing; D007202:Indicators and Reagents; D030821:Plants, Genetically Modified / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000096:biosynthesis*; D018749:RNA, Plant / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics*; D011995:Recombination, Genetic
D007202:Indicators and Reagents; D012330:RNA, Double-Stranded; D018749:RNA, Plant; D034741:RNA, Small Interfering