Lineage-specific amino acid substitutions in region 2 of the RNA polymerase sigma subunit affect the temperature of promoter opening.
Highly conserved amino acid residues in region 2 of the RNA polymerase sigma subunit are known to participate in promoter recognition and opening. We demonstrated that nonconserved residues in this region collectively determine lineage-specific differences in the temperature of promoter opening.
Barinova
N
N
Institute of Molecular Genetics, Kurchatov Sq. 2, Moscow 123182, Russia.
Zhilina
E
E
Bass
I
I
Nikiforov
V
V
Kulbachinskiy
A
A
D019943:Amino Acid Substitution / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism; D034301:Deinococcus / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics* / Q000378:metabolism*; D013696:Temperature; D013824:Thermus / Q000201:enzymology / Q000235:genetics; D014158:Transcription, Genetic
D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases