title: A sensitive rutin assay using a simple probe manganese sulfate based on its novel resonance light scattering decrease phenomenon.
abstract: A novel resonance light scattering (RLS) decrease method was developed to determine rutin with a simple probe manganese sulfate. At pH 7.5, the strong RLS intensity of manganese sulfate was remarkably decreased by the addition of rutin with the maximum peak located at 389.0 nm. Under the optimum conditions, a good linear relationship between the changes of RLS intensities of manganese sulfate with and without rutin and the concentrations of rutin was obtained over the range of 0.49-24.4 microg ml(-1) and a low detection limit (3sigma) 0.42 microg ml(-1) was achieved in the mean time. Based on this approach, a novel method for quantitative analysis of rutin is proposed in this paper. The method proposed was also applied successfully to the determination of rutin in commercial pharmaceutical preparations of compound rutin tablets and human urine samples. The assay is sensitive, rapid, inexpensive, practical and relatively free from interference generated by coexisting substances.
authors.affiliation: Department of Chemistry, Shantou University, Shantou 515063, China. kqlu@stu.edu.cn
authors.forename: Zhanguang
authors.initials: Z
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Junhui
authors.initials: J
authors.lastname: Chen
authors.forename: Jinbin
authors.initials: J
authors.lastname: Liu
authors.forename: Yali
authors.initials: Y
authors.lastname: Han
mesh_terms: D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D017895:Manganese Compounds / Q000032:analysis* / Q000737:chemistry*; D015336:Molecular Probe Techniques; D015394:Molecular Structure; D012431:Rutin / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013431:Sulfates / Q000032:analysis* / Q000737:chemistry*; D013997:Time Factors; D014556:Urine / Q000737:chemistry
chemical_list: D017895:Manganese Compounds; D013431:Sulfates; D012431:Rutin; C039798:manganese sulfate