Purification of monoclonal antibodies.
The uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A-Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion-exchange chromatography for the preparation of contaminant-free antibody.
Fuller
S A
SA
Allelix Inc., Mississauga, Ontario.
Takahashi
M
M
Hurrell
J G
JG
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology; D002846:Chromatography, Affinity; D007158:Immunologic Techniques
D000911:Antibodies, Monoclonal