title: Structural basis for ubiquitin recognition by the Otu1 ovarian tumor domain protein.
abstract: Ubiquitination of proteins modifies protein function by either altering their activities, promoting their degradation, or altering their subcellular localization. Deubiquitinating enzymes are proteases that reverse this ubiquitination. Previous studies demonstrate that proteins that contain an ovarian tumor (OTU) domain possess deubiquitinating activity. This domain of approximately 130 amino acids is weakly similar to the papain family of proteases and is highly conserved from yeast to mammals. Here we report structural and functional studies on the OTU domain-containing protein from yeast, Otu1. We show that Otu1 binds polyubiquitin chain analogs more tightly than monoubiquitin and preferentially hydrolyzes longer polyubiquitin chains with Lys(48) linkages, having little or no activity on Lys(63)- and Lys(29)-linked chains. We also show that Otu1 interacts with Cdc48, a regulator of the ER-associated degradation pathway. We also report the x-ray crystal structure of the OTU domain of Otu1 covalently complexed with ubiquitin and carry out structure-guided mutagenesis revealing a novel mode of ubiquitin recognition and a variation on the papain protease catalytic site configuration that appears to be conserved within the OTU family of ubiquitin hydrolases. Together, these studies provide new insights into ubiquitin binding and hydrolysis by yeast Otu1 and other OTU domain-containing proteins.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Troy Eugene
authors.initials: TE
authors.lastname: Messick
authors.forename: Nathaniel Scott
authors.initials: NS
authors.lastname: Russell
authors.forename: Ayaka Jennifer
authors.initials: AJ
authors.lastname: Iwata
authors.forename: Kathryn Lorenz
authors.initials: KL
authors.lastname: Sarachan
authors.forename: Ramin
authors.initials: R
authors.lastname: Shiekhattar
authors.forename: John R
authors.initials: JR
authors.lastname: Shanks
authors.forename: Francisca E
authors.initials: FE
authors.lastname: Reyes-Turcu
authors.forename: Keith D
authors.initials: KD
authors.lastname: Wilkinson
authors.forename: Ronen
authors.initials: R
authors.lastname: Marmorstein
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005656:Fungal Proteins / Q000737:chemistry; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013869:Thiolester Hydrolases / Q000737:chemistry*; D025801:Ubiquitin / Q000737:chemistry*; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D025801:Ubiquitin; C496399:OTUB2 protein, human; D013869:Thiolester Hydrolases; D003546:Cysteine Endopeptidases; D000251:Adenosine Triphosphatases; C000614177:CDC48 protein, S cerevisiae; D000074405:Valosin Containing Protein