[RAPD and SCAR molecular markers linked to the sexuality of cycads (Cycas tanqingii D. Y. Wang)].
The random amplified polymorphic DNA (RAPD) technique was used to amplify DNA fragment, aiming at finding markers linked to the sex trait in Cycas tanqingii D. Y. Wang. A total number of 160 random primers were screened in the RAPD-PCR and more than 2500 RAPD fragments were generated from the male or the female plants. One fragment of about 500 bp was amplified steadily and repeatedly by the S0465 (CCCCGGTAAC) primer only from female plants but not male plants. The RAPD marker was then converted into female-linked dominant SCAR (Sequence Characterized Amplified Regions) marker named STQC-S465-483. The development of this sex-linked SCAR marker provides a possibility of identifying the sex of Cycas tanqingii before sexual maturation, which is very important to in situ or ex situ conservation.
Jing
Jian-Zhou
JZ
College of Food and Biology Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China. jzjing@zzuli.edu.cn
Jin
Hong
H
Li
Dong-Liang
DL
Chen
Xiao-Ke
XK
Zhang
Yong
Y
D001483:Base Sequence; D029784:Cycas / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D019849:Sex Determination Processes
D005819:Genetic Markers