[Impact of the hCG on epidermal growth factor in the phallic tissues of hypospadiac mice].
OBJECTIVE
To investigate the impact of hCG on the epidermal growth factor (EGF) in the phallic tissues of hypospadiac mice.


METHODS
The Kunming mouse model of congenital hypospadias was established. As controls, 10 three-week-old normal male mice and 10 3-week-old congenital hypospadiac mice were injected with isometric sodium chloride intraperitoneally. Another 40 mice with hypospadias were divided into 4 groups and injected intraperitoneally with 50 IU, 100 IU, 150 IU and 200 IU of hCG respectively per day for 7 days. Then the concentration of ECG in the phallus and in the serum were assayed by ELISA.


RESULTS
The EGF level in the phallus of the hypospadiac mice was much lower than that of the controls statistically (P < 0. 05). After the injection of exogenous hCG, the concentration of ECG increased with the dose of hCG, 50 IU (59.57 +/- 22.16) pg/ml, 100 IU (57.97 +/- 9.59) pg/ml, 150 IU (73.02 +/- 31.35) pg/ml and 200 IU (92.45 +/- 35.74) pg/ml. The concentration of ECG showed no significant difference between the control group and the 150 IU and 2000 IU groups (P > 0.05).


CONCLUSIONS
Decreased concentration of EGF in the mouse phallus may be associated with the pathogeny of hypospadias. A proper dose of exogenous hCG may increase the concentration of EGF in the mouse phallus.
Yang
Xiao-Feng
XF
Department of Urology, the First Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China. yxfylq@163.com
Han
Xue-Bing
XB
Rong
Dong-Wen
DW
Li
De-Biao
DB
D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004815:Epidermal Growth Factor / Q000096:biosynthesis* / Q000097:blood; D007021:Hypospadias / Q000097:blood / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D010413:Penis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
D006063:Chorionic Gonadotropin; D004815:Epidermal Growth Factor