Design, synthesis, and amplification of DNA pools for construction of combinatorial pools and libraries.
This unit describes the design, synthesis, and amplification of random-sequence DNA pools, from which functional nucleic acid-binding or catalytic species can be selected. Since it is an expensive and time-consuming process, the authors have provided an extensive strategic planning section to guide investigators in designing and constructing the pool.
Pollard
J
J
Mintz Levin Cohn Glovsky Ferris and Popeo, Boston, Massachusetts, USA.
Bell
S D
SD
Ellington
A D
AD
D001483:Base Sequence; D001665:Binding Sites; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004247:DNA / Q000138:chemical synthesis / Q000235:genetics*; D017931:DNA Primers; D015195:Drug Design; D005784:Gene Amplification; D015723:Gene Library; D012313:RNA / Q000235:genetics; D012107:Research Design
D017931:DNA Primers; D012313:RNA; D004247:DNA