In field-grown coffee trees source-sink manipulation alters photosynthetic rates, independently of carbon metabolism, via alterations in stomatal function.
Perturbations of the source-sink balances were performed in field-grown coffee (Coffea arabica) trees to investigate the possible role of carbohydrates in feedback regulation of photosynthesis. Four treatments were applied at the whole-plant level: (i) complete defruiting and maintenance of the full leaf area, (ii) the half crop load and full leaf area, (iii) the full crop load and full leaf area and (iv) the full crop load and half leaf area. Sampling and measurements were performed twice during the phase of dry matter accumulation of fruits. Gas exchange, chlorophyll a fluorescence, carbon isotope labelling and steady-state metabolite measurements were assessed in source leaves. The average rate of net photosynthetic rate (A) and stomatal conductance (g(s)) were larger (> 50%), and carbon isotope composition ratio was lower, in trees with a full crop load and half leaf area than in defruited trees, with individuals of the other two treatments showing intermediate values. However, differences in A seem unlikely to have been caused either by photochemical impairments or a direct end-product-mediated feedback down-regulation of photosynthesis. It is proposed that the decreased A in defruited coffee trees was independent of carbon metabolism and was rather directly related to a lower CO(2) availability coupled to lower g(s).
DaMatta
Fábio M
FM
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
Cunha
Roberto L
RL
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
Antunes
Werner C
WC
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
Martins
Samuel C V
SCV
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
Araujo
Wagner L
WL
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
Fernie
Alisdair R
AR
Max-Planck-Institute for Molecular Plant Physiology, Am Muelenberg 1, 14476 Potsdam-Golm, Germany.
Moraes
Gustavo A B K
GABK
Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-000 Viçosa, MG, Brasil.
D002244:Carbon / Q000378:metabolism*; D040503:Coffea / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology; D005638:Fruit / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology*; D054046:Plant Stomata / Q000502:physiology*; D018526:Plant Transpiration / Q000502:physiology; D012621:Seasons; D013997:Time Factors
D002244:Carbon