title: A novel role for PA28gamma-proteasome in nuclear speckle organization and SR protein trafficking.
abstract: In eukaryotic cells, proteasomes play an essential role in intracellular proteolysis and are involved in the control of most biological processes through regulated degradation of key proteins. Analysis of 20S proteasome localization in human cell lines, using ectopic expression of its CFP-tagged alpha7 subunit, revealed the presence in nuclear foci of a specific and proteolytically active complex made by association of the 20S proteasome with its PA28gamma regulator. Identification of these foci as the nuclear speckles (NS), which are dynamic subnuclear structures enriched in splicing factors (including the SR protein family), prompted us to analyze the role(s) of proteasome-PA28gamma complexes in the NS. Here, we show that knockdown of these complexes by small interfering RNAs directed against PA28gamma strongly impacts the organization of the NS. Further analysis of PA28gamma-depleted cells demonstrated an alteration of intranuclear trafficking of SR proteins. Thus, our data identify proteasome-PA28gamma complexes as a novel regulator of NS organization and function, acting most likely through selective proteolysis. These results constitute the first demonstration of a role of a specific proteasome complex in a defined subnuclear compartment and suggest that proteolysis plays important functions in the precise control of splicing factors trafficking within the nucleus.
authors.affiliation: Centre de Recherche de Biochimie Macromoléculaire (CRBM-CNRS UMR 5237) and Institut de Génétique Moléculaire de Montpellier (IGMM-CNRS UMR 5535), IFR122, Universités Montpellier 1 et 2, Montpellier, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Baldin
authors.forename: Muriel
authors.initials: M
authors.lastname: Militello
authors.forename: Yann
authors.initials: Y
authors.lastname: Thomas
authors.forename: Christine
authors.initials: C
authors.lastname: Doucet
authors.forename: Weronika
authors.initials: W
authors.lastname: Fic
authors.forename: Stephanie
authors.initials: S
authors.lastname: Boireau
authors.forename: Isabelle
authors.initials: I
authors.lastname: Jariel-Encontre
authors.forename: Marc
authors.initials: M
authors.lastname: Piechaczyk
authors.forename: Edouard
authors.initials: E
authors.lastname: Bertrand
authors.forename: Jamal
authors.initials: J
authors.lastname: Tazi
authors.forename: Olivier
authors.initials: O
authors.lastname: Coux
mesh_terms: D021581:Active Transport, Cell Nucleus; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D046912:Multiprotein Complexes; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D061988:Proteasome Inhibitors; D021122:Protein Subunits; D034741:RNA, Small Interfering / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001324:Autoantigens; C485182:Cyan Fluorescent Protein; C099558:Ki antigen; D046912:Multiprotein Complexes; D061988:Proteasome Inhibitors; D021122:Protein Subunits; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D046988:Proteasome Endopeptidase Complex