Technical note: improved ancient DNA purification for PCR using ion-exchange columns.
A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples.
Kim
Kijeong
K
Institute for Medical Sciences, Chung-Ang University, Seoul 156-756, South Korea.
Kim
Kyung-Yong
KY
Jeon
Eunhee
E
Togloom
Ariunaa
A
Cho
Youn-Ock
YO
Lee
Min-Soo
MS
Lkhagvasuren
Gavaachimed
G
Choi
Jee-Hye
JH
Tumen
Dashtseveg
D
Ja Park
Ae
A
Kim
Keun-Cheol
KC
Park
Ki-Won
KW
Kim
Jae-Hyun
JH
Noh
Maengseok
M
Yoo
Kwon-Jong
KJ
Lee
Kwang-Ho
KH
D001842:Bone and Bones; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000302:isolation & purification*; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D004247:DNA