Efficient purification of small unsaturated oligoglucuronides by reversed-phase chromatography.
Ion-exchange chromatography has been applied to purification of unsaturated oligoglucuronans. After an isocratic elution on a strong anion-exchange column, the collected fractions were desalted by low pressure size exclusion chromatography. However, this efficient separation was limited by the time required to desalt. So, we developed a reversed-phase chromatography method using back ionization of oligomers. Two C18 columns were tested with trifluoroacetic acid (TFA 0.7%) as eluent. Different selectivities and column stabilities were observed in this acidic condition. The scale up for semi-preparative applications enabled us to recover pure unsaturated oligoglucuronans without desalting step.
Tavernier
Marie-Laure
ML
Laboratoire des Glucides, UMR CNRS 6219, Université de Picardie Jules Verne, 33 rue Saint-Leu, F-80039 Amiens, France.
Petit
Emmanuel
E
Lesur
David
D
Wadouachi
Anne
A
Courtois
Bernard
B
Courtois
Josiane
J
Michaud
Philippe
P
D000837:Anion Exchange Resins / Q000737:chemistry*; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D007475:Ion Exchange Resins / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000837:Anion Exchange Resins; D005965:Glucuronates; D007475:Ion Exchange Resins; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase