The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors.
It has been reported that genes encoding antigens of bacterial and viral pathogens can be expressed in plants and are shown to induce protection antibodies. The structural protein E2 of classical swine fever virus (CSFV), which has been shown to carry critical epitopes, has been expressed in different systems. Here, we report the expression of CFSV E2 gene in tobacco chloroplasts. Mice immunized with leaf extracts elicited specific antibodies. This indicated that the expressed E2 proteins had a certain degree of immunogenicity. To our knowledge, this is the first report showing induction of protective antibody in response to classical swine fever virus (CSFV) by immunization with antigen protein E2 expressed in tobacco chloroplasts, which will open a new way to protection from CSFV by plant chloroplasts as bioreactors.
Shao
Hong-Bo
HB
Binzhou University, Binzhou 256603, China; Institute of Soil and Water Conservation, Chinese Academy of Sciences & Water Resources Ministry (CAS&WRM), Yangling 712100, China. shaohongbochu@126.com
He
Dong-Mei
DM
Qian
Kai-Xian
KX
Shen
Gui-Fang
GF
Su
Zhong-Liang
ZL
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D019149:Bioreactors; D015139:Blotting, Southern; D015153:Blotting, Western; D002736:Chloroplasts / Q000378:metabolism*; D006692:Classical Swine Fever Virus / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D005822:Genetic Vectors; D007114:Immunization; D051379:Mice; D010936:Plant Extracts / Q000276:immunology; D018515:Plant Leaves / Q000276:immunology; D016133:Polymerase Chain Reaction; D014026:Nicotiana / Q000648:ultrastructure*; D014170:Transformation, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D010936:Plant Extracts; D014759:Viral Envelope Proteins