Assessment of sera for chromatin-immunoprecipitation.
Chromatin-immunoprecipitation (ChIP) is a powerful technique for mapping the protein-DNA interactions that occur in living cells. The critical technical determinant for successful ChIP is the availability of an appropriate, "ChIP-grade" serum. Here we present a technique designed to assess whether sera are suitable for ChIP, and to quantify their efficiency relative to a positive internal reference. This approach is useful as a first step toward ChIP-on-chip or ChIP-sequencing, especially in the case of recently identified proteins for which no binding sites are yet known.
Nguyen
Juliette
J
Centre National de la Recherche Scientifique, Paris, France.
Yamada
Daisuke
D
Schultz
David C
DC
Defossez
Pierre-Antoine
PA
D002460:Cell Line; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004268:DNA-Binding Proteins / Q000378:metabolism; D017930:Genes, Reporter; D006801:Humans; D012097:Repressor Proteins / Q000378:metabolism; D044967:Serum
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C508974:ZBTB4 protein, human