Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (Pisum sativum L.).
Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as in biotic and abiotic stress responses. They are classified according to their sequence homology into four major groups (A-D). A large amount of information about MAPKs in groups A and B is available but few data of the C group have been reported. In this study, a C1 subgroup MAP kinase cDNA, PsMPK2, was isolated from Pisum sativum. PsMPK2 is expressed in vegetative (root and leaf) and reproductive (stamen, pistil and fruit) organs. Expression of PsMPK2 in Arabidopsis thaliana shows that mechanical injury and other stress signals as abscisic acid, jasmonic acid and hydrogen peroxide increase its kinase activity, extending previous results indicating that C1 subgroup MAPKs may be involved in the response to stress.
Ortiz-Masia
Dolores
D
Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andrés Estellés s/n, 46100, Burjassot, Spain.
Perez-Amador
Miguel A
MA
Carbonell
Pablo
P
Aniento
Fernando
F
Carbonell
Juan
J
Marcote
Maria J
MJ
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007700:Kinetics; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000201:enzymology*; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D020928:Mitogen-Activated Protein Kinases