title: Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis.
abstract: Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A(1) activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.
authors.affiliation: National Research Laboratory of Plant Developmental Genetics, Department of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Youbong
authors.initials: Y
authors.lastname: Hyun
authors.forename: Sungwook
authors.initials: S
authors.lastname: Choi
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Hwang
authors.forename: Jihyeon
authors.initials: J
authors.lastname: Yu
authors.forename: Sang-Jip
authors.initials: SJ
authors.lastname: Nam
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Ko
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Park
authors.forename: Young Sam
authors.initials: YS
authors.lastname: Seo
authors.forename: Eun Yu
authors.initials: EY
authors.lastname: Kim
authors.forename: Stephen Beungtae
authors.initials: SB
authors.lastname: Ryu
authors.forename: Woo Taek
authors.initials: WT
authors.lastname: Kim
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Lee
authors.forename: Heonjoong
authors.initials: H
authors.lastname: Kang
authors.forename: Ilha
authors.initials: I
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D002736:Chloroplasts / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D014644:Genetic Variation; D009928:Organ Specificity; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010739:Phospholipase D / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054466:Phospholipases A1 / Q000378:metabolism; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000648:ultrastructure; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D002265:Carboxylic Ester Hydrolases; C019262:galactolipase; C445094:DAD1 protein, Arabidopsis; D010741:Phospholipases A; D054466:Phospholipases A1; D010739:Phospholipase D