title: Technical note: improved ancient DNA purification for PCR using ion-exchange columns.
abstract: A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples.
authors.affiliation: Institute for Medical Sciences, Chung-Ang University, Seoul 156-756, South Korea.
authors.forename: Kijeong
authors.initials: K
authors.lastname: Kim
authors.forename: Kyung-Yong
authors.initials: KY
authors.lastname: Kim
authors.forename: Eunhee
authors.initials: E
authors.lastname: Jeon
authors.forename: Ariunaa
authors.initials: A
authors.lastname: Togloom
authors.forename: Youn-Ock
authors.initials: YO
authors.lastname: Cho
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Lee
authors.forename: Gavaachimed
authors.initials: G
authors.lastname: Lkhagvasuren
authors.forename: Jee-Hye
authors.initials: JH
authors.lastname: Choi
authors.forename: Dashtseveg
authors.initials: D
authors.lastname: Tumen
authors.forename: Ae
authors.initials: A
authors.lastname: Ja Park
authors.forename: Keun-Cheol
authors.initials: KC
authors.lastname: Kim
authors.forename: Ki-Won
authors.initials: KW
authors.lastname: Park
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Maengseok
authors.initials: M
authors.lastname: Noh
authors.forename: Kwon-Jong
authors.initials: KJ
authors.lastname: Yoo
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D001842:Bone and Bones; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000302:isolation & purification*; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA