title: Simultaneous investigation of the influence of topography and charge on protein adsorption using artificial nanopatterns.
abstract: The combined influence of surface topography and charge of a polymer surface on the adsorption of the protein avidin has been investigated. Atomic force microscopy contact mode imaging and charge writing were used to create defined topographical roughness and electrostatic charge patterns on the surface of polystyrene. Increased avidin adsorption was found on nanometer-size topographical patterns, but the adsorption remained unaffected by electrostatic patterns.
authors.affiliation: London Centre for Nanotechnology and Department of Medicine, University College London, 5 University Street, London WC1E 6JJ, United Kingdom.
authors.forename: E Macarena
authors.initials: EM
authors.lastname: Blanco
authors.forename: Michael A
authors.initials: MA
authors.lastname: Horton
authors.forename: Patrick
authors.initials: P
authors.lastname: Mesquida
mesh_terms: D000327:Adsorption; D001360:Avidin / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D011137:Polystyrenes; D001360:Avidin