title: Proteomic profiling of aging in the mouse heart: Altered expression of mitochondrial proteins.
abstract: Using two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometry, we have used a systems biology approach to study the molecular basis of aging of the mouse heart. We have identified 8 protein spots whose expression is up-regulated due to aging and 36 protein spots whose expression is down-regulated due to aging (p0.05 as judged by Wilcoxon Rank Sum test). Among the up-regulated proteins, we have characterized 5 protein spots and 2 of them, containing 3 different enzymes, are mitochondrial proteins. Among the down-regulated proteins, we have characterized 27 protein spots and 16 of them are mitochondrial proteins. Mitochondrial damage is believed to be a key factor in the aging process. Our current study provides molecular evidence at the level of the proteome for the alteration of structural and functional parameters of the mitochondria that contribute to impaired activity of the mouse heart due to aging.
authors.affiliation: Keck Graduate Institute of Applied Life Sciences, Proteomics Center, 535 Watson Drive, Claremont, CA 91711, USA. bulbul_chakravarti@kgi.edu
authors.forename: Bulbul
authors.initials: B
authors.lastname: Chakravarti
authors.forename: Melva
authors.initials: M
authors.lastname: Oseguera
authors.forename: Neville
authors.initials: N
authors.lastname: Dalal
authors.forename: Pamela
authors.initials: P
authors.lastname: Fathy
authors.forename: Buddhadeb
authors.initials: B
authors.lastname: Mallik
authors.forename: Alpan
authors.initials: A
authors.lastname: Raval
authors.forename: Deb N
authors.initials: DN
authors.lastname: Chakravarti
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008929:Mitochondria, Heart / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D040901:Proteomics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry