[Effect of low molecular heparin sodium cream on the content of serum nitric oxide in random pattern skin flap: experiment with rats].
OBJECTIVE
To explore the mechanism of improvement of blood circulation in random pattern skin flap by low molecular heparin sodium cream.


METHODS
48 Wistar rats underwent formation of random skin flap of the size of 2 cm x 8 cm on the back and then were randomly divided into 2 equal groups: experiment group with low molecular heparin sodium cream smeared on the skin flaps and control group with Vaseline smeared on the skin flaps. 24, 48, and 72 hours, and 7 days after the smearing blood samples were collected from 6 rats respectively to detect the content of serum nitric oxide a (NO). Seven days after the smearing specimens were collected from the upper, middle, and lower parts of the skin flaps to undergo pathological examination.


RESULTS
(1) The serum NO content of the experiment group was (53 +/- 15) micromol/L, significantly higher than that of the control group [(27 +/- 20) micromol/L, P < 0.05] 7 days after the operation. (2) The skin flap survival rate of the experiment group was (66 +/- 18)%, significantly higher than that of the control group [(22 +/- 16)%, P < 0.01]. (3) Histomorphology showed formation of neo-vessels with integrated endangium of capillary, stability of structure of mitochondria, and milder cell swelling in the flaps treated with heparin cream.


CONCLUSIONS
Low molecular heparin sodium cream increases the content of serum NO, thus increasing the survival of skin flaps.
He
Yin-Fei
YF
Department of Orthopedics, Seventh People's Hospital of Datong, Datong 037007, China.
Wang
Ya-Rong
YR
Li
Li-Fen
LF
Wang
Shu-Hua
SH
Ye
Jin-Wen
JW
D000818:Animals; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D008297:Male; D008954:Models, Biological; D009569:Nitric Oxide / Q000097:blood*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000098:blood supply / Q000187:drug effects*; D016038:Skin Transplantation; D013524:Surgical Flaps / Q000098:blood supply*
D006495:Heparin, Low-Molecular-Weight; D009569:Nitric Oxide