title: Adsorption of a statherin peptide fragment on the surface of nanocrystallites of hydroxyapatite.
abstract: Statherin is an active inhibitor of calcium phosphate precipitation in the oral cavity. For many studies of the interaction between statherin and hydroxyapatite (HAp), the samples are prepared by a direct mixing of statherin or its fragment with well-crystalline HAp crystals. In this work, the HAp sample is precipitated in the presence of peptide fragment derived from the N-terminal 15 amino acids of statherin (SN-15). The in situ prepared HAp crystallites are nanosized, leading to a significant increase of the peptide amount adsorbed on the HAp surface. The enhancement in NMR sensitivity allows, for the first time, the measurement of a two-dimensional 13C-13C correlation spectrum for a 13C uniformly labeled peptide sample adsorbed on mineral surface. The measurement time is about 18.5 h at a field strength of 7.05 T. Preliminary results suggest that there may exist two different mechanisms for the interaction between SN-15 and HAp. In addition to the one which will cause a conformational change near the N-terminal, SN-15 may also be absorbed on the HAp surface by simple electrostatic interaction, without any significant conformational changes of the peptides.
authors.affiliation: Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
authors.forename: Peng-Huan
authors.initials: PH
authors.lastname: Chen
authors.forename: Yao-Hung
authors.initials: YH
authors.lastname: Tseng
authors.forename: Yun
authors.initials: Y
authors.lastname: Mou
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Tsai
authors.forename: Syuan-Ming
authors.initials: SM
authors.lastname: Guo
authors.forename: Shing-Jong
authors.initials: SJ
authors.lastname: Huang
authors.forename: Steve S-F
authors.initials: SS
authors.lastname: Yu
authors.forename: Jerry C C
authors.initials: JC
authors.lastname: Chan
mesh_terms: D000327:Adsorption; D002247:Carbon Isotopes; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods / Q000592:standards; D049329:Nanostructures / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry*; D012015:Reference Standards; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D002247:Carbon Isotopes; D010446:Peptide Fragments; C018149:STATH protein, human; D012471:Salivary Proteins and Peptides; D017886:Durapatite