Improvement of biodiesel production by lipozyme TL IM-catalyzed methanolysis using response surface methodology and acyl migration enhancer.
The process of biodiesel production from corn oil catalyzed by lipozyme TL IM, an inexpensive 1,3-position specific lipase from Thermomyces lanuginosus was optimized by response surface methodology (RSM) and a central composite rotatable design (CCRD) was used to study the effects of enzyme dosage, ratio of t-butanol to oil (v/v) and ratio of methanol to oil (mol/mol) on the methyl esters (ME) yield of the methanolysis. The optimum combinations for the reaction were 25.9U/goil of enzyme, 0.58 volume ratio of t-butanol to oil and 0.5, 0.5, 2.8 molar equivalent of methanol to oil added at the reaction time of 0, 2, and 4h, respectively, by which a ME yield of 85.6%, which was very close to the predicted value of 85.0%, could be obtained after reaction for 12h. Waste oil was found to be more suitable feedstock, and could give 93.7% ME yield under the optimum conditions described above. Adding triethylamine (TEA), an acyl migration enhancer, could efficiently improve the ME yield of the methanolysis of corn oil, giving a ME yield of 92.0%.
Wang
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Y
Laboratory of Applied Biocatalysis, South China University of Technology, Guangzhou 510640, PR China.
Wu
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H
Zong
M H
MH
D001674:Bioelectric Energy Sources; D002384:Catalysis; D002849:Chromatography, Gas; D003314:Corn Oil / Q000737:chemistry; D008049:Lipase / Q000737:chemistry; D008697:Methane / Q000737:chemistry*; D013499:Surface Properties
D003314:Corn Oil; C093666:Lipozyme; D008049:Lipase; D008697:Methane