title: A microfluidic system for dynamic yeast cell imaging.
abstract: The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.
authors.affiliation: CellASIC Corporation, San Leandro, CA 94577, USA. pjlee@cellasic.com
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Lee
authors.forename: Noah C
authors.initials: NC
authors.lastname: Helman
authors.forename: Wendell A
authors.initials: WA
authors.lastname: Lim
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Hung
mesh_terms: D049452:Green Fluorescent Proteins / Q000378:metabolism; D008565:Membrane Proteins; D044085:Microfluidics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: C490024:FUS1 protein, S cerevisiae; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins