title: Active pharmaceutical ingredients and mechanisms underlying phasic myometrial contractions stimulated with the saponin extract from Paris polyphylla Sm. var. yunnanensis used for abnormal uterine bleeding.
abstract: BACKGROUND
  Total steroidal saponins of Paris polyphylla Sm. var. yunnanensis (TSSP) have been widely used in China for the treatment of abnormal uterine bleeding (AUB). But until now, the main active constituents and the mechanisms underlying the pharmacological actions on uterine activity have not been described.
  
  
  METHODS
  Total steroidal saponins were extracted with EtOH and purified by chromatography. In vitro isometric contraction studies were performed using myometrial strips from estrogen-primed or pregnant rats. Intracellular calcium was monitored under a confocal microscope using Fluo-3 AM-loaded myometrial cells.
  
  
  RESULTS
  TSSP dose-dependently induced phasic myometrial contractions in vitro. Experiments with calcium channel blockers or kinase inhibitors demonstrated that the TSSP-stimulated myometrial contraction was mediated by an increase in [Ca(2+)](i) via influx of extracellular calcium and release of intracellular calcium. Through bioassay-guided separation, it was found that total spirostanol saponins exhibited contractile activity in myometrium and Pennogenin-3-O-alpha-L-arabinofuranosyl(1-->4)[alpha-L-rhamnopyranosyl(1-->2)]-beta-D-glucopyranoside (PARG) was identified as the active ingredient of TSSP. Furthermore, the contractile response of rat myometrium to PARG was significantly enhanced with advancing pregnancy.
  
  
  CONCLUSIONS
  These data provide evidence that myometrial contractility stimulated by TSSP results from [Ca(2+)](i) increase and supports the possibility that some spirostanol gylcosides may represent a new type of contractile agonist for the uterus.
authors.affiliation: Department of Pathophysiology, Beijing Institute of Radiation Medicine, No. 27 Taiping Road, Beijing 100850, China.
authors.forename: L
authors.initials: L
authors.lastname: Guo
authors.forename: J
authors.initials: J
authors.lastname: Su
authors.forename: B W
authors.initials: BW
authors.lastname: Deng
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Yu
authors.forename: L P
authors.initials: LP
authors.lastname: Kang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Y J
authors.initials: YJ
authors.lastname: Shan
authors.forename: J P
authors.initials: JP
authors.lastname: Chen
authors.forename: B P
authors.initials: BP
authors.lastname: Ma
authors.forename: Y W
authors.initials: YW
authors.lastname: Cong
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D008796:Metrorrhagia / Q000188:drug therapy*; D010120:Oxytocics / Q000494:pharmacology*; D010936:Plant Extracts; D028321:Plant Preparations; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D014590:Uterine Contraction / Q000187:drug effects*
chemical_list: D010120:Oxytocics; D010936:Plant Extracts; D028321:Plant Preparations; D012503:Saponins