Control of Aspergillus section Flavi growth and aflatoxin accumulation by plant essential oils.
OBJECTIVE
The antifungal effect of Pimpinella anisum (anise), Pëumus boldus (boldus), Mentha piperita (peppermint), Origanum vulgare (oregano) and Minthosthachys verticillata (peperina) essential oils against Aspergillus section Flavi (two isolates of Aspergillus parasiticus and two isolates of Aspergillus flavus) was evaluated in maize meal extract agar at 0.982 and 0.955 water activities, at 25 degrees C.


RESULTS
The percentage of germination, germ-tube elongation rate, growth rate and aflatoxin B(1) (AFB(1)) accumulation at different essential oils concentrations were evaluated. Anise and boldus essential oils were the most inhibitory at 500 mg kg(-1) to all growth parameters of the fungus. These essential oils inhibited the percentage of germination, germ-tube elongation rate and fungal growth. AFB(1) accumulation was completely inhibited by anise, boldus and oregano essential oils. Peperina and peppermint essential oils inhibited AFB(1) production by 85-90% in all concentrations assayed.


CONCLUSIONS
Anise and boldus essential oils could be considered as effective fungitoxicans for Aspergillus section flavi.


CONCLUSIONS
Our results suggest that these phytochemical compounds could be used alone or in conjunction with other substances to control the presence of aflatoxigenic fungi in stored maize.
Bluma
R
R
Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales. Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
Amaiden
M R
MR
Daghero
J
J
Etcheverry
M
M
D000348:Aflatoxins / Q000378:metabolism*; D001231:Aspergillus flavus / Q000187:drug effects / Q000254:growth & development*; D004305:Dose-Response Relationship, Drug; D005516:Food Microbiology; D005659:Fungicides, Industrial / Q000494:pharmacology*; D018525:Germination / Q000187:drug effects; D036142:Mentha piperita; D009822:Oils, Volatile / Q000494:pharmacology*; D031341:Origanum; D031611:Peumus; D028042:Pimpinella; D010938:Plant Oils / Q000494:pharmacology*; D013172:Spores, Fungal / Q000187:drug effects; D003313:Zea mays / Q000382:microbiology
D000348:Aflatoxins; D005659:Fungicides, Industrial; D009822:Oils, Volatile; D010938:Plant Oils