Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions.
Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.
Westblade
Lars F
LF
The Rockefeller University, New York, New York 10065, USA.
Minakhin
Leonid
L
Kuznedelov
Konstantin
K
Tackett
Alan J
AJ
Chang
Emmanuel J
EJ
Mooney
Rachel A
RA
Vvedenskaya
Irina
I
Wang
Qing Jun
QJ
Fenyö
David
D
Rout
Michael P
MP
Landick
Robert
R
Chait
Brian T
BT
Severinov
Konstantin
K
Darst
Seth A
SA
D000595:Amino Acid Sequence; D017122:Bacteriophage T4 / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000821:virology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D012321:DNA-Directed RNA Polymerases