Development of the reverse passive latex agglutination method for the detection and quantification of the genus Nitrospira in the wastewater treatment process.
This report describes a new immunological method for the detection and quantification of Nitrospira populations using the reverse passive latex agglutination (RPLA). The numbers of the genus Nitrospira have been quantified only by molecular biological techniques such as FISH and quantitative PCR to date. Using high-density latex particles and a specific polyclonal antibody, Nitrospira populations in the wastewater treatment process were quantified in the shortest 4 h of incubation. The minimum detectable number of Nitrospira cells was 7.0x10(5) (log(10) 5.85) cells/ml. It is thought that the RPLA method can quantify Nitrospira populations more simply, economically, and speedily than molecular biological techniques or the culture method, because this procedure has a simple protocol and does not require the use of specialized equipment, expensive reagents, or technical skill. Therefore it is applicable for use in the everyday control and maintenance of water quality in wastewater treatment facilities where equipment is not sufficient or in the field.
Okumura
Takekazu
T
Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186-8650, Japan. takekazu-okumura@yakult.co.jp
Nagai
Fumiko
F
Yamamoto
Shuta
S
Hashimoto
Toshikazu
T
Ito
Masahiko
M
Sawada
Haruji
H
D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers; D017404:In Situ Hybridization, Fluorescence; D007841:Latex Fixation Tests / Q000379:methods*; D016133:Polymerase Chain Reaction; D014871:Water Microbiology
D017931:DNA Primers