title: [Progress in the derivation, culture of human embryonic stem cells].
abstract: Human embryonic stem (hES) cells are considered to be a valuable resource for research in regenerative medicine, drug screening, and developmental studies. However, hES cells are usually established and maintained on mouse embryonic fibroblast feeder layers, and the risk of animal origin contamination from feeder layer generally excludes the clinical use of these hES cells. The main emphasis over the last several years has been in finding defined serum-and feeder layer-free system for derivation and culture of hES cells to enable the clinical use of hES cell for cell transplantation.
authors.affiliation: Department of Reproductive Endocrinology, The Obstetrics and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Chen-ming
authors.initials: CM
authors.lastname: Xu
authors.forename: He-feng
authors.initials: HF
authors.lastname: Huang
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D018920:Coculture Techniques; D016895:Culture Media, Serum-Free; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology; D013234:Stem Cells / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D019737:Transplants
chemical_list: D016895:Culture Media, Serum-Free; D014157:Transcription Factors