Yeast Polo-like kinase substrates are nailed with the right tools.
A platform has been built combining chemical genetics and bioinformatics to screen the proteome for physiological substrates of the Polo-like kinase in budding yeast. A novel role for this kinase in regulating the mitotic spindle is revealed.
Archambault
Vincent
V
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK. va228@cam.ac.uk
Glover
David M
DM
D018797:Cell Cycle Proteins / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D005656:Fungal Proteins; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D004718:Saccharomycetales / Q000201:enzymology*; D008941:Spindle Apparatus; D013379:Substrate Specificity; D000097869:Polo-Like Kinase 1
D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D017346:Protein Serine-Threonine Kinases; D020543:Proteome; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1