[Effect of hepatitis C virus core protein on cholangiocarcinoma tissues' epithelial-mesenchymal transition].
OBJECTIVE
To explore the role of hepatitis C virus core protein on the infiltration and metastasis of cholangiocarcinoma tissues.


METHODS
From January 2001 to November 2006, 34 patients with cholangiocarcinoma who had intact follow-up data randomly were chosen. The expression of HCVc protein, epithelium markers and mesenchymal markers in cholangiocarcinoma tissues were examined by SP methods of immunohistochemistry, clinical-pathological data were recorded and analyzed.


RESULTS
The positive expression rate was observed in 47.1% for HCVc protein, 50% for N-cadherin, 44.1% for Vimentin, 55.9% for Fibronectin and the decreased expression rate was E-cadherin for 55.9%, alpha-catenin for 70.6%, beta-catenin for 55.9%. The positive expression of HCVc protein was associated with the decreased expression of E-cadherin, alpha-catenin and the positive expression of N-cadherin, Vimentin, Fibronectin (chi(2) = 4.480, 4.163, 4.250, 7.438, 12.260, P < 0.05). A positive-correlation between the expression of HCVc protein and metastasis of lymph nodes and other organs were found (chi(2) = 5.708, 4.163, P < 0.05).


CONCLUSIONS
HCVc protein might promote cholangiocarcinoma tissues' infiltration and metastasis by inducing it's epithelial-mesenchymal transition.
Li
Tian-yu
TY
Hepatobiliary Surgery Institute of People Liberation Army, South-west Hospital of the Third Military Medical University, Chongqing 400038, China.
Wang
Shu-guang
SG
Li
Da-jiang
DJ
Gao
Zhan-feng
ZF
Gao
Ying-hong
YH
Jiang
Wei-wei
WW
D000328:Adult; D000368:Aged; D002471:Cell Transformation, Neoplastic; D018281:Cholangiocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D005260:Female; D016174:Hepacivirus / Q000378:metabolism; D006526:Hepatitis C / Q000378:metabolism / Q000473:pathology / Q000821:virology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014758:Viral Core Proteins / Q000378:metabolism / Q000502:physiology*
D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus