Distribution of L-azetidine-2-carboxylate N-acetyltransferase in yeast.
The budding yeast Saccharomyces cerevisiae Sigma1278b contains the MPR1 gene encoding N-acetyltransferase, which detoxifies the L-proline analog L-azetidine-2-carboxylate (AZC). Of 131 yeasts tested, AZC acetyltransferase activity was detected in 17 strains of 41 strains that showed AZC resistance. Degenerate-PCR analysis revealed that two strains, i.e., Candida saitoana AKU4533 and Wickerhamia fluorescens AKU4722, contained a DNA fragment highly homologous to MPR1. This indicates that AZC acetyltransferases are widely distributed in yeasts.
Wada
Masaru
M
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. wada@chem.agr.hokudai.ac.jp
Okabe
Kae
K
Kataoka
Michihiko
M
Shimizu
Sakayu
S
Yokota
Atsushi
A
Takagi
Hiroshi
H
D001383:Azetidinecarboxylic Acid / Q000378:metabolism*; D005800:Genes, Fungal; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D001383:Azetidinecarboxylic Acid