title: Purification, crystallization and preliminary crystallographic study of a recombinant plant aminoaldehyde dehydrogenase from Pisum sativum.
abstract: Aminoaldehydes are products of polyamine degradation and are known to be reactive metabolites that are toxic to living cells at high concentrations. These compounds are catabolized by aminoaldehyde dehydrogenases, which are enzymes that contain a nicotinamide adenine dinucleotide coenzyme. Aminoaldehyde dehydrogenase from Pisum sativum was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop method. A complete data set was collected to 2.8 A resolution at 100 K. Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 86.4, b = 216.6, c = 205.4 A, beta = 98.1 degrees. Molecular replacement was performed and led to the identification of six dimers per asymmetric unit.
authors.affiliation: Department of Biochemistry, Faculty of Science, Palacký University, Slechtitelů 11, CZ-78371 Olomouc, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Tylichová
authors.forename: Pierre
authors.initials: P
authors.lastname: Briozzo
authors.forename: David
authors.initials: D
authors.lastname: Kopecný
authors.forename: Julien
authors.initials: J
authors.lastname: Ferrero
authors.forename: Solange
authors.initials: S
authors.lastname: Moréra
authors.forename: Nathalie
authors.initials: N
authors.lastname: Joly
authors.forename: Jacques
authors.initials: J
authors.lastname: Snégaroff
authors.forename: Marek
authors.initials: M
authors.lastname: Sebela
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D018532:Pisum sativum / Q000201:enzymology*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D000444:Aldehyde Dehydrogenase