title: Techniques for mammalian cell tissue culture.
abstract: This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
authors.affiliation: Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Phelan
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D005615:Freezing; D007202:Indicators and Reagents; D007246:Infertility; D014357:Trypsin
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D014357:Trypsin