Glycosidase profiling with immobilised glycosidase-inhibiting iminoalditols--a proof-of-concept study.
Three typical glycosidase-inhibiting iminoalditols were attached to a polyamine surface displayed on a silicon chip. Exposure to a representative beta-glucosidase revealed selective binding events reflecting the different structural features of the inhibitors probed in this study. This provides a proof-of-concept for the successful exploitation of microarrays of typical reversible glycosidase inhibitors of the iminosugar family.
Steiner
Andreas J
AJ
Glycogroup, Institut für Organische Chemie,Technische Universität Graz, Stremayrgasse 16, A-8010 Graz, Austria.
Stütz
Arnold E
AE
Wrodnigg
Tanja M
TM
Tarling
Chris A
CA
Withers
Stephen G
SG
Hermetter
Albin
A
Schmidinger
Hannes
H
D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D050111:Imino Sugars / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D046228:Microarray Analysis; D015394:Molecular Structure; D012231:Rhizobium / Q000201:enzymology
D004791:Enzyme Inhibitors; D050111:Imino Sugars; D006026:Glycoside Hydrolases