Preparing and using M13-derived vectors.
Many vectors in current use are derived from filamentous phages. These vectors are used because DNA inserted into them can be recovered in two forms: double-stranded circles and single-stranded circles. This overview unit describes the lifecycle of filamentous phages along with the development and use of filamentous phage vectors.
Greenstein
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Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Besmond
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D017104:Bacteriophage M13 / Q000235:genetics* / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D008967:Molecular Biology / Q000379:methods*