Subcellular location of horseradish peroxidase in horseradish leaves treated with La(III), Ce(III) and Tb(III).
The agricultural application of rare-earth elements (REEs) would promote REEs inevitably to enter in the environment and then to threaten the environmental safety and human health. Therefore, the distribution of the REEs ion, (141)Ce(III) and effects of La(III), Ce(III) and Tb(III) on the distribution of horseradish peroxidase (HRP) in horseradish mesophyll cells were investigated with electron microscopic radioautography and transmission electron microscopic cytochemistry. It was found for the first time that REEs ions can enter into the mesophyll cells, deposit in both extra and intra-cellular. Compared to the normal condition, after the horseradish leaves treated with La(III) or Tb(III), HRP located on the tonoplast is decreased and HRP is mainly located on the cell wall, while HRP is mainly located on the plasma membrane after the horseradish leaves were treated with Ce(III). This also indicated that REEs ions may regulate the plant growth through changing the distribution of enzymes.
Ye
Yaxin
Y
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
Wang
Lihong
L
Huang
Xiaohua
X
Lu
Tianhong
T
Ding
Xiaolan
X
Zhou
Qing
Q
Guo
Shaofen
S
D031215:Armoracia / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002563:Cerium / Q000378:metabolism / Q000633:toxicity; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D007811:Lanthanum / Q000378:metabolism / Q000633:toxicity; D058503:Mesophyll Cells / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008674:Metals, Rare Earth / Q000378:metabolism / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism*; D013725:Terbium / Q000378:metabolism / Q000633:toxicity
D004785:Environmental Pollutants; D008674:Metals, Rare Earth; D013725:Terbium; D002563:Cerium; D007811:Lanthanum; D006735:Horseradish Peroxidase