Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms.
Alloxazine can bind to adenine selectively over other nucleobases opposite an abasic site in DNA duplexes (5'-TCC AGX GCA AC-3'/3'-AGG TCN CGT TG-5', X=AP site, N=A, T, C, G) with a dissociation constant of 0.82 microM (pH 7.0, I=0.11 M, at 5 degrees C), and it is applicable to SNPs typing of PCR amplification products based on the binding-induced fluorescence response.
Rajendar
Burki
B
Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai 980-8578, Japan.
Nishizawa
Seiichi
S
Teramae
Norio
N
D000225:Adenine / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005415:Flavins / Q000737:chemistry*; D005838:Genotype; D007700:Kinetics; D008024:Ligands; D020641:Polymorphism, Single Nucleotide; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D013816:Thermodynamics
D005415:Flavins; D008024:Ligands; C008173:isoalloxazine; D004247:DNA; D000225:Adenine