[Cytotoxicity of MTA, Dycal and GIC on human periodontal ligament cells in vitro].
OBJECTIVE
To compare the cytotoxicity of three endodontic materials (MTA, Dycal and GIC) on human periodontal ligament cells (HPDLCs) in vitro.


METHODS
HPDLCs proliferation was evaluated using methylthiazol tetrazolium (MTT) assay for three endodontic materials in different concentration. SAS6.12 software package was used to analyse the results.


RESULTS
There was no statistically significant difference in three subgroups of MTA group (P>0.05). Statistically significant differences were found in Dycal group (P<0.05). In GIC group, there was no statistically significant difference between the control and 10 mg/ml subgroup (P>0.05). At the same time, statistically significant differences were found in between the control and 20 mg/ml or 40 mg/ml subgroup (P<0.05).


CONCLUSIONS
Dycal and GIC are more cytotoxic than MTA. MTA is superior to Dycal and GIC in biocompatibility.
Xia
Lie
L
Department of Operative Dentistry & Endodontics, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
Jiang
Long
L
Zhu
Ya-qin
YQ
D002126:Calcium Hydroxide / Q000633:toxicity*; D049109:Cell Proliferation; D005971:Glutamates / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives* / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D008903:Minerals / Q000633:toxicity*; D000068437:Pemetrexed; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects; D013778:Tetrazolium Salts
D005971:Glutamates; D008903:Minerals; D013778:Tetrazolium Salts; D000068437:Pemetrexed; C025702:Dycal; D006147:Guanine; D002126:Calcium Hydroxide