Novel acetylated alpha-cyclosophorotridecaose produced by Ralstonia solanacearum.
Alpha-cyclosophorotridecaose (alpha-C13) produced by Ralstonia solanacearum is isolated by trichloroacetic acid treatment and subjected to various chromatographic techniques. Here, we report for the first time that R. solanacearum produces acetylated alpha-C13. Structural analyses of the acetylated alpha-C13 were performed with 1D or 2D NMR spectroscopy, MALDI-TOF MS and HPLC. The results show that the alpha-C13 is substituted by mainly one acetyl residue at the C-6 position of the glucose unit.
Cho
Eunae
E
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul, Republic of Korea.
Lee
Sanghoo
S
Jung
Seunho
S
D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D043368:Ralstonia solanacearum / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003505:Cyclodextrins