title: Mutant bovine odorant-binding protein: Temperature affects the protein stability and dynamics as revealed by infrared spectroscopy and molecular dynamics simulations.
abstract: Bovine odorant-binding protein (bOBP), a member of the lipocalin family, presents the so-called 3D "domain-swapped" protein structure. In fact, in solution, it appears as a dimer in which each monomer is composed by the classical lipocalin fold, with a central beta-barrel followed by a stretch of residues and the alpha-helix domain protruding out of the barrel and crossing the dimer interface. Recently, a deswapped mutant form of bOBP was obtained, in which a Gly residue was inserted after position 121 and the two residues in position 64 and 156 were replaced by Cys residues for restoring the disulfide bridge common to the lipocalin family. In this work, we used Fourier transform infrared spectroscopy and molecular dynamics simulations to investigate the effect of temperature on the structural stability and conformational dynamics of the mutant bOBP. The spectroscopic and molecular simulation data pointed out that the hydrophobic regions of the protein matrix appear to be an important factor for the protein stability and integrity. In addition, it was also found that the mutant bOBP is significantly stabilized by the binding of the ligand, which may have an impact on the biological function of bOBP. The obtained results will allow for a better use of this protein as probe for the design of advanced protein-based biosensors for the detection of compounds used in the fabrication of explosive powders.
authors.affiliation: Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Marabotti
authors.forename: Thierry
authors.initials: T
authors.lastname: Lefèvre
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.forename: Roberta
authors.initials: R
authors.lastname: Crescenzo
authors.forename: Antonio
authors.initials: A
authors.lastname: Varriale
authors.forename: Mosè
authors.initials: M
authors.lastname: Rossi
authors.forename: Michel
authors.initials: M
authors.lastname: Pézolet
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
mesh_terms: D000818:Animals; D002417:Cattle; D009154:Mutation; D018035:Receptors, Odorant / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013055:Spectrophotometry, Infrared / Q000379:methods*
chemical_list: D018035:Receptors, Odorant; C045648:odorant-binding protein