Fractionation and analysis of neutral oligosaccharides by HPLC.
This unit describes the fractionation and analysis of neutral oligosaccharides by high-performance liquid chromatography (HPLC) on bonded amine columns. Separation is based upon hydrogen bonding between the NH2 groups of the column and the hydroxyl groups of the oligosaccharides. A support protocol describes the reduction and desalting of neutral oligosaccharides with sodium borohydride.
Hayes
B K
BK
University of Alabama at Birmingham, Birmingham, Alabama, USA.
Varki
A
A
D000588:Amines / Q000737:chemistry; D000818:Animals; D001894:Borohydrides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007477:Ions / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D012492:Salts / Q000737:chemistry
D000588:Amines; D001894:Borohydrides; D007477:Ions; D009844:Oligosaccharides; D012492:Salts; C025364:sodium borohydride