Experimental rat lung tumor model with intrabronchial tumor cell implantation.
OBJECTIVE
The objective of this study was to develop a rat lung tumor model for anticancer drug testing.


METHODS
Sixty-two female Wistar rats weighing 208 +/- 20 g were anesthetized intraperitoneally with 2.5% tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5 x 10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival.


RESULTS
The tumor take rate was 94.7% for implants with 4 x 10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8%.


CONCLUSIONS
The present rat lung tumor model was shown to be feasible: the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.
Gomes Neto
Antero
A
Federal University of Ceará, Brazil. anterogn@terra.com.br
Simão
Antônio Felipe Leite
AF
Miranda
Samuel de Paula
Sde P
Mourão
Lívia Talita Cajaseiras
LT
Bezerra
Nilfácio Prado
NP
Almeida
Paulo Roberto Carvalho de
PR
Ribeiro
Ronaldo de Albuquerque
Rde A
D000818:Animals; D002279:Carcinoma 256, Walker / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D016014:Linear Models; D008175:Lung Neoplasms / Q000473:pathology* / Q000556:secondary; D051381:Rats; D014057:Tomography, X-Ray Computed / Q000379:methods; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods