title: Expression profiling of senescent-associated genes in human dermis from young and old donors. Proof-of-concept study.
abstract: It is often described that it is difficult to really discriminate the cause of intrinsic skin aging. The aim of this study was to compare the profiles of expression of senescence-associated genes in biopsies of dermis from young and old human donors. TGF-beta1 was up-regulated in the dermis of old donors as well as the TGF-beta1-regulated genes. The anti-oxidant enzymes Selenium-dependent Glutathione peroxidase and Glutatione S-Transferase Theta 1 were also up-regulated in old dermis as well as Tumor Necrosis Factor Receptor Superfamily 1A. None of these genes had altered expression level in skin fibroblasts embedded in a collagen matrix and exposed to sublethal doses of UVB, suggesting their involvement in intrinsic aging. This study represents a proof-of-concept of larger whole transcriptome studies where all avenues should be used to subtract changes in gene expression due to extrinsic aging from changes potentially due to intrinsic aging.
authors.affiliation: Research Unit on Cellular Biology (URBC), Department of Biology, Faculty of Sciences, University of Namur (FUNDP), Rue de Bruxelles, 61, 5000 Namur, Belgium.
authors.forename: Céline
authors.initials: C
authors.lastname: Borlon
authors.forename: Geoffroy
authors.initials: G
authors.lastname: Weemaels
authors.forename: Patrice
authors.initials: P
authors.lastname: Godard
authors.forename: Florence
authors.initials: F
authors.lastname: Debacq-Chainiaux
authors.forename: Pierre
authors.initials: P
authors.lastname: Lemaire
authors.forename: Christophe
authors.initials: C
authors.lastname: Deroanne
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
mesh_terms: D000375:Aging / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D005982:Glutathione Transferase