title: Absence of apolipoprotein B-3500 mutation in Turkish patients with coronary and cerebrovascular atherosclerosis.
abstract: OBJECTIVE
  The arginine- to-glutamine change at codon 3500 of the apolipoprotein B-100 (apo B) is a well- known genetic cause of hypercholesterolemia. Since increased cholesterol levels lead to atherosclerosis, identification of the carries of the apo B-3500 mutation is an important step in the risk stratification of individuals and families with hypercholesterolemia. The prevalence of this mutation in Turkish population is not well known. We aimed to investigate the frequency of apo B-100 mutations (codon 3500) C9774T (Arg 3500-->Trp) and G9775A (Arg 3500-->Gln) in patients with atherosclerosis in comparison with healthy subjects.
  
  
  METHODS
  This cross-sectional study included 442 unrelated subjects living on the West coast of Turkey. Subgroups consisted of 165 patients with coronary artery disease, 163 patients with ischemic stroke, and 114 healthy control subjects.
  
  
  RESULTS
  We did not find any apo B-100 mutation both in the patient and control groups.
  
  
  CONCLUSIONS
  As it is hypothesized that this mutation arose within the Central European region from a common ancestor approximately 7000 years ago and spread across Europe, our result of the absence of the R3500Q mutation in Turkish patients give an important information about the geographical distribution of the apo B-R3500Q, that the mutation has not reached to Anatolia.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey.
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Eroğlu
authors.forename: Nur
authors.initials: N
authors.lastname: Selvi
authors.forename: Buket
authors.initials: B
authors.lastname: Kosova
authors.forename: Cigir
authors.initials: C
authors.lastname: Biray
authors.forename: Emre
authors.initials: E
authors.lastname: Kumral
authors.forename: Nejat
authors.initials: N
authors.lastname: Topçuoğlu
authors.forename: Meral
authors.initials: M
authors.lastname: Kayikçioğlu
mesh_terms: D000328:Adult; D053299:Apolipoprotein B-100 / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D003324:Coronary Artery Disease / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D003430:Cross-Sectional Studies; D004247:DNA / Q000032:analysis; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D002537:Intracranial Arteriosclerosis / Q000097:blood / Q000453:epidemiology / Q000235:genetics; D008297:Male; D008875:Middle Aged; D017354:Point Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D015995:Prevalence; D014280:Triglycerides / Q000097:blood; D014421:Turkey / Q000453:epidemiology; D044465:White People / Q000235:genetics
chemical_list: D053299:Apolipoprotein B-100; D008078:Cholesterol, LDL; D014280:Triglycerides; D004247:DNA; D002784:Cholesterol