Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus).
In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, expected heterozygosity was 0.869 and the mean polymorphic information content value was 0.847. Paternity exclusion probabilities over loci were NE-1P = 0.01336 and NE-2P = 0.00135, and combined non-exclusion probability of identity was P(ID) = 3 x 10(-8).
Cosse
M
M
Departamento de Genética - IIBCE Unidad Asociada de Facultad de Ciencias, Montevideo, Uruguay. mcosse@iibce.edu.uy
González
S
S
Maldonado
J E
JE
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003670:Deer / Q000145:classification / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005828:Genetics, Population; D006041:Goats / Q000235:genetics; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D012756:Sheep / Q000235:genetics; D013045:Species Specificity
D017931:DNA Primers