title: Beveled fiber-optic probe couples a ball lens for improving depth-resolved fluorescence measurements of layered tissue: Monte Carlo simulations.
abstract: In this study, we evaluate the feasibility of designing a beveled fiber-optic probe coupled with a ball lens for improving depth-resolved fluorescence measurements of epithelial tissue using Monte Carlo (MC) simulations. The results show that by using the probe configuration with a beveled tip collection fiber and a flat tip excitation fiber associated with a ball lens, discrimination of fluorescence signals generated in different tissue depths is achievable. In comparison with a flat-tip collection fiber, the use of a large bevel angled collection fiber enables a better differentiation between the shallow and deep tissue layers by changing the excitation-collection fiber separations. This work suggests that the beveled fiber-optic probe coupled with a ball lens has the potential to facilitate depth-resolved fluorescence measurements of epithelial tissues.
authors.affiliation: Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore.
authors.forename: Franck
authors.initials: F
authors.lastname: Jaillon
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Huang
mesh_terms: D004848:Epithelium; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005453:Fluorescence; D007909:Lenses; D008954:Models, Biological; D009010:Monte Carlo Method; D055100:Optical Fibers; D011522:Protons
chemical_list: D011522:Protons