title: Influence of hemoglobin vesicles, cellular-type artificial oxygen carriers, on human umbilical cord blood hematopoietic progenitor cells in vitro.
abstract: Hemoglobin vesicles (HbVs), liposomal oxygen carriers containing human hemoglobin, are candidates for development as clinically useful blood substitutes. Although HbVs are shown to distribute transiently into the bone marrow in animal models, the influence of HbVs on human hematopoietic stem/progenitor cells has not yet been studied. Therefore, we investigated the influence of HbVs at a concentration of up to 3 vol/vol % on the clonogenic activity (in semisolid culture) and proliferative activity (in liquid culture) of human hematopoietic progenitor cells derived from umbilical cord blood (CB) in vitro. Continuous exposure of CB mononuclear cells to HbVs tended to decrease the number and size of mature-committed colonies and most notably reduced the number of colonies of high-proliferative potential colony-forming cells (HPP-CFC). In contrast, exposure to HbVs for 20 h or 3 days, which is more relevant to the clinical setting, had no effect on the number of mature-committed colonies and only modestly decreased the number of HPP-CFC. Continuous exposure (10 days) to HbVs significantly suppressed the cellular proliferation and differentiation of both the erythroid and myeloid lineages in liquid culture. Again, short exposure (20 h or 3 days) did not affect these parameters. Thus, our results show that HbVs, under conditions relevant to the clinical setting, have no adverse effect on human CB hematopoietic progenitor activity in vitro.
authors.affiliation: Japanese Red Cross, Hokkaido Red Cross Blood Center, Yamanote 2-2, Nishi-Ku, Sapporo 063-0002, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Fujihara
authors.forename: Shinobu
authors.initials: S
authors.lastname: Wakamoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sakai
authors.forename: Shinji
authors.initials: S
authors.lastname: Takeoka
authors.forename: Eishun
authors.initials: E
authors.lastname: Tsuchida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Azuma
authors.forename: Hisami
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D001802:Blood Substitutes / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D049109:Cell Proliferation / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology* / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006454:Hemoglobins / Q000494:pharmacology*; D006801:Humans; D008081:Liposomes; D010100:Oxygen / Q000378:metabolism; D013997:Time Factors
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D010100:Oxygen