Micafungin combined with fluconazole, an effective therapy for murine blastoschizomycosis.
OBJECTIVE
To study the efficacy of micafungin combined with fluconazole in a murine model of disseminated blastoschizomycosis.


METHODS
Mice were treated with fluconazole at 40 or 80 mg/kg/day given orally, with micafungin at 10 mg/kg/day given subcutaneously or with a combination of micafungin with fluconazole at the doses described above. Treatment began 1 day after infection and continued for 6 days post-infection. Tissue burden studies were performed 1 day after the treatment finished. The experiments were performed with three different strains.


RESULTS
The two combinations tested significantly improved the survival of mice with respect to the control group and reduced the tissue burden significantly with respect to the control and their respective monotherapies in most organs tested. All animals that received the combination of micafungin with fluconazole at 80 mg/kg/day survived up to the end of the experiment.


CONCLUSIONS
The combination of micafungin with fluconazole is promising for the treatment of disseminated blastoschizomycosis.
Serena
Carolina
C
Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
Rodríguez
M Mar
MM
Mariné
Marçal
M
Pastor
F Javier
FJ
Guarro
Josep
J
D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001759:Blastomycosis / Q000188:drug therapy*; D015169:Colony Count, Microbial; D004359:Drug Therapy, Combination; D054714:Echinocandins / Q000008:administration & dosage / Q000627:therapeutic use*; D015725:Fluconazole / Q000008:administration & dosage / Q000627:therapeutic use*; D007668:Kidney / Q000382:microbiology; D055666:Lipopeptides; D008074:Lipoproteins / Q000008:administration & dosage / Q000627:therapeutic use*; D008099:Liver / Q000382:microbiology; D008297:Male; D000077551:Micafungin; D051379:Mice; D008826:Microbial Sensitivity Tests; D013154:Spleen / Q000382:microbiology; D016019:Survival Analysis
D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D008074:Lipoproteins; D015725:Fluconazole; D000077551:Micafungin