Oxidation by hypochlorite converts protective HDL into a potent platelet agonist.
High density lipoproteins (HDL) represent a protective factor of central importance that counteracts the development of cardiovascular disease, in part by normalizing platelet (hyper)reactivity. As HDL represent an efficient scavenger of the naturally occurring oxidant hypochlorite, this work was intended to investigate the influence of hypochlorite-oxidized HDL on platelet function. Addition of hypochlorite-oxidized HDL to human platelets results in an immediate and transient raise in intracellular calcium, surface expression of P-selectin and platelet aggregation. The observed effects are dose dependent and can be blocked by an antibody directed against the lipoprotein-binding domain of platelet thrombospondin- and scavenger receptor CD36.
Assinger
Alice
A
Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Schwarzspanierstrasse 17, Vienna, Austria.
Schmid
Werner
W
Eder
Sandra
S
Schmid
Diethart
D
Koller
Elisabeth
E
Volf
Ivo
I
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008075:Lipoproteins, HDL / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D019007:P-Selectin / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects
D008075:Lipoproteins, HDL; D019007:P-Selectin; D000244:Adenosine Diphosphate; D006997:Hypochlorous Acid; D002118:Calcium