title: Purification of monoclonal antibodies.
abstract: The uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A-Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion-exchange chromatography for the preparation of contaminant-free antibody.
authors.affiliation: Allelix Inc., Mississauga, Ontario.
authors.forename: S A
authors.initials: SA
authors.lastname: Fuller
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: J G
authors.initials: JG
authors.lastname: Hurrell
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001201:Ascites / Q000276:immunology; D002846:Chromatography, Affinity; D007158:Immunologic Techniques
chemical_list: D000911:Antibodies, Monoclonal