Protective effect of resveratrol against naphthalene-induced oxidative stress in mice.
OBJECTIVE
This investigation confirms the role of free radicals in naphthalene-induced toxicity and elucidates the mechanism of resveratrol (RVT).


METHODS
Both male and female BALB-c mice were administered with naphthalene (100 mg/kg, i.p.) for 30 days, either along with saline or along with RVT (10mg/kg, orally). At the end of the experiment, following treatment and sacrifice of animals by decapitation, lung, liver and kidney tissue samples were taken for histological examination or determination of malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO) activity and collagen contents. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine levels and lactate dehydrogenase (LDH) activity were measured in the serum samples, while TNF-alpha, IL-beta, IL-6 and total antioxidant capacity (AOC) were assayed in plasma samples.


RESULTS
Naphthalene administration caused a significant decrease in tissue GSH and plasma AOC, which was accompanied with significant increases in tissue MDA and collagen levels and MPO activity. Moreover, the pro-inflammatory mediators (TNF-alpha, IL-beta, IL-6), LDH activity, AST, ALT, creatinine and BUN levels were significantly increased in the naphthalene group. On the other hand, RVT treatment reversed all these biochemical indices as well as histopathological alterations induced by naphthalene.


CONCLUSIONS
Oxidative mechanisms play an important role in naphthalene-induced tissue damage, and RVT, by inhibiting neutrophil infiltration, balancing oxidant-antioxidant status, and regulating the generation of inflammatory mediators, ameliorates oxidative organ injury due to naphthalene toxicity.
Sehirli
Ozer
O
Department of Pharmacology, Marmara University School of Pharmacy, Istanbul, Turkey.
Tozan
Ayfer
A
Omurtag
Gülden Z
GZ
Cetinel
Sule
S
Contuk
Gazi
G
Gedik
Nursal
N
Sener
Göksel
G
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009281:Naphthalenes / Q000633:toxicity*; D009504:Neutrophils / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
D000975:Antioxidants; D004785:Environmental Pollutants; D009281:Naphthalenes; D013267:Stilbenes; C031721:naphthalene; D008315:Malondialdehyde; D005978:Glutathione; D000077185:Resveratrol