title: A rapid, microplate SNP genotype assay for the leptinob allele.
abstract: A nonsense mutation in the mouse leptin gene causes genetic obesity. As a result of extensive research in the field of obesity, the use of leptinob mice is widespread. This mutation renders mice sterile, creating the need to breed heterozygous mice. For this reason, leptinob genotyping is necessary. To date, gel-based assays have been used for genotyping. Using the Invader Plus assay for single nucleotide polymorphism (SNP) detection, we have developed a gel-free microplate SNP assay for genotyping leptinwt and leptinob alleles.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
authors.forename: Angie T
authors.initials: AT
authors.lastname: Oler
authors.forename: Alan D
authors.initials: AD
authors.lastname: Attie
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D020738:Leptin / Q000235:genetics*; D051379:Mice; D008820:Mice, Obese / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D020738:Leptin; D004247:DNA