Heme-iron oxygenases: powerful industrial biocatalysts?
Are cytochrome P450 enzymes powerful industrial biocatalysts? Next to market demands, well-defined enzyme functionalities and process parameters allow generalizations on the basis of process windows. These can provide useful guidelines for the design of improved biocatalysts. Oxygenase-catalyzed reactions are of special interest for selective C-H bond oxidation. The versatile class of cytochrome P450 mono-oxygenases attracts particular attention, and impressive advances have been achieved with respect to mechanistic insight, enzyme activity, stability, and specificity. Recent major achievements include significant increases in productivities, yields, and rates of catalytic turnover as well as modification of substrate specificity and efficient multistep reactions in whole-cell biocatalysts. For some biocatalysts, these parameters are already of an industrially useful magnitude.
Julsing
Mattijs K
MK
Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Strasse 66, D-44221 Dortmund, Germany.
Cornelissen
Sjef
S
Bühler
Bruno
B
Schmid
Andreas
A
D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002617:Chemical Industry / Q000379:methods*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015202:Protein Engineering
D003577:Cytochrome P-450 Enzyme System