Cellular localization of insulin-like growth factor-II protein in the sea bass (Dicentrarchus labrax) from hatching to adult.
The cellular localization of IGF-II protein was investigated during larval and postlarval developmental stages of sea bass (Dicentrarchus labrax) by immunohistochemistry using antisera raised against Sparus aurata IGF-II. At hatching, IGF-II immunoreactivity was already present in the skin, developing intestine and skeletal muscle. During larval life IGF-II protein was also observed in heart musculature, in kidney and gill epithelia as well as in liver. In fry skeletal muscle a moderate IGF-II immunostaining was detected in red fibres, whereas white muscle fibres exhibited a faint immunoreactivity. In adults, a marked IGF-II immunostaining was observed in red muscle fibres. A moderate immunoreactivity was also present in white fibres as well as in heart striated myocardial fibres. These results are in agreement with previous findings on the spatial localization of IGF-II and IGF type 1 receptor in S. aurata and Umbrina cirrosa, confirming the role of IGF system during development and growth of fish.
Radaelli
G
G
Department of Experimental Veterinary Sciences, University of Padua, Italy. giuseppe.radaelli@unipd.it
Poltronieri
C
C
Bertotto
D
D
Funkenstein
B
B
Simontacchi
C
C
D000818:Animals; D001492:Bass / Q000254:growth & development / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D007124:Immunoenzyme Techniques; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000378:metabolism*; D008018:Life Cycle Stages
D015415:Biomarkers; D007335:Insulin-Like Growth Factor II