Mouse embryonic stem (ES) cell culture.
Mouse embryonic stem (ES) cells are used to generate mouse mutants by gene targeting and blastocyst-mediated transgenesis. ES cells must be cultured under conditions that prevent differentiation to maintain their ability to transmit altered alleles by contributing to the germ line. This unit describes one of the most common methods for culturing ES cells using mouse embryo fibroblast (MEF) feeder layers and recombinant leukemia inhibitory factor (LIF) to prevent differentiation.
Conner
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Harvard Medical School, Boston, Massachusetts, USA.
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D007202:Indicators and Reagents; D051379:Mice; D013234:Stem Cells / Q000166:cytology*
D003470:Culture Media; D007202:Indicators and Reagents