title: IgG deposition and activation of the classical complement pathway involvement in the activation of human granulocytes by decellularized porcine heart valve tissue.
abstract: Decellularization treatment of heart valves has been thought to eliminate tissue immunogenicity. Early failure of tissue-engineered xenogeneic heart valves was seen in children and has been a major drawback in this promising field of research. This study was designed to characterize the effects of acellular porcine heart valve tissue on immune activation in vitro. Incubation of decellularized porcine tissue with human plasma led to adsorption of IgG, activation of the classical complement pathway and adhesion of activated polymorphonuclear leukocytes (PMN). This inflammatory response was strongly inhibited by proteins extracted from native porcine tissue which might indicate that inhibitors of PMN activation present in the extracellular matrix (ECM) are lost during the decellularization process.
authors.affiliation: Department of Cardiothoracic Surgery, Medical University Vienna, Vienna, Austria.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Bastian
authors.forename: Marie-Elisabeth
authors.initials: ME
authors.lastname: Stelzmüller
authors.forename: Klaus
authors.initials: K
authors.lastname: Kratochwill
authors.forename: Marie-Theres
authors.initials: MT
authors.lastname: Kasimir
authors.forename: Paul
authors.initials: P
authors.lastname: Simon
authors.forename: Guenter
authors.initials: G
authors.lastname: Weigel
mesh_terms: D000368:Aged; D000818:Animals; D002474:Cell-Free System / Q000276:immunology; D002478:Cells, Cultured; D003167:Complement Activation / Q000276:immunology*; D003171:Complement Pathway, Classical / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006351:Heart Valves / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D013552:Swine
chemical_list: D007074:Immunoglobulin G