New bioactive biomaterials based on quaternized chitosan.
Chitosan was chemically modified to produce quaternary ammonium salts in order to improve its antimicrobial activity and physicochemical properties. Quaternization of N-alkyl chitosan derivatives was carried out using alkyl iodide to elaborate water-soluble cationic polyelectrolytes ( N, N, N-trimethylchitosan, TMC). TMC was characterized by (1)H NMR spectroscopy; the quaternization degree was determined from (1)H NMR spectra and by titration of iodide ion. The antibacterial activity of hydroxypropylcellulose (HPC) films or coatings associated with chitosan or TMC as biocide was evaluated against the growth of Listeria monocytogenes and Salmonella typhimurium. The HPC-chitosan and HPC-TMC coatings exhibited a total inhibition on solid medium of both bacterial strains. Experiments conducted in liquid medium showed that the inhibitory activity against the growth of Listeria innocua was improved after chemical modification. Moreover, physicochemical properties of films were evaluated to determine their potential for food applications. The addition of the antibacterial agents showed a significant impact on the moisture barrier and mechanical properties of HPC films.
Belalia
Rachid
R
Université Bordeaux 1, INRA, CNRS, UMR 5103 US2B, 351 cours de la liberation, 33405 Talence, France.
Grelier
Stéphane
S
Benaissa
Mohammed
M
Coma
Véronique
V
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D018857:Food Packaging; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development; D013329:Structure-Activity Relationship
D000900:Anti-Bacterial Agents; D000644:Quaternary Ammonium Compounds; D048271:Chitosan