title: Preparing and using M13-derived vectors.
abstract: Many vectors in current use are derived from filamentous phages. These vectors are used because DNA inserted into them can be recovered in two forms: double-stranded circles and single-stranded circles. This overview unit describes the lifecycle of filamentous phages along with the development and use of filamentous phage vectors.
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: D
authors.initials: D
authors.lastname: Greenstein
authors.forename: C
authors.initials: C
authors.lastname: Besmond
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics* / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D008967:Molecular Biology / Q000379:methods*