Transduction of the rat brain by Bovine Herpesvirus 4.
Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFP Delta TK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat glioma cells. BoHV-4EGFP Delta TK was injected into the lateral ventricle of the rat brain. Histology and immunohistochemistry showed that ependymal and rostral migratory stream cells were transduced while neurons were not. Clinical scores, evaluated for 90 days, indicated that the virus was non neuropathogenic, suggesting this virus is a suitable vector for brain tumor gene therapy.
Redaelli
Marco
M
Department of Human Anatomy and Physiology, University of Padova, 35131 Padova, Italy. marco.redaelli@unipd.it
Cavaggioni
Andrea
A
Mucignat-Caretta
Carla
C
Cavirani
Sandro
S
Caretta
Antonio
A
Donofrio
Gaetano
G