Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15.
We analyzed 43 CTX-M-15-producing Escherichia coli isolates and 6 plasmids encoding the blaCTX-M-15 gene from Canada, India, Kuwait, France, Switzerland, Portugal, and Spain. Most isolates belonged to phylogroups B2 (50%) and D (25%). An EC-B2 strain of clonal complex sequence type (ST) 131 was detected in all countries; other B2 isolates corresponded to ST28, ST405, ST354, and ST695 from specific areas. EC-D strains were clonally unrelated but isolates from 3 countries belonged to ST405. All CTX-M-15 plasmids corresponded to IncFII group with overrepresentation of 3 HpaI-digested plasmid DNA profiles (A, B and C; 85-120kb, similarity > or =70%). Plasmid A was detected in EC-B2 strains (ST131, ST354, or ST405), plasmid C was detected in B2 and D strains, and plasmid B was confined to worldwide-disseminated ST131. Most plasmids contained blaOXA-1, aac(6')-Ib-cr, and blaTEM-1. Worldwide dissemination of CTX-M-15 seems to be determined by clonal complexes ST131 and ST405 and multidrug-resistant IncFII plasmids.
Coque
Teresa M
TM
Hospital Universitario Ramón y Cajal, Madrid, Spain. mcoque.hrc@salud.madrid.org
Novais
Angela
A
Carattoli
Alessandra
A
Poirel
Laurent
L
Pitout
Johann
J
Peixe
Luísa
L
Baquero
Fernando
F
Cantón
Rafael
R
Nordmann
Patrice
P
D002170:Canada / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D029968:Escherichia coli Proteins / Q000235:genetics; D005060:Europe / Q000453:epidemiology; D006801:Humans; D007194:India / Q000453:epidemiology; D007730:Kuwait / Q000453:epidemiology; D010957:Plasmids / Q000235:genetics*; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
D029968:Escherichia coli Proteins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases