title: Transduced human PEP-1-heat shock protein 27 efficiently protects against brain ischemic insult.
abstract: Reactive oxygen species contribute to the development of various human diseases. Ischemia is characterized by both significant oxidative stress and characteristic changes in the antioxidant defense mechanism. Heat shock protein 27 (HSP27) has a potent ability to increase cell survival in response to oxidative stress. In the present study, we have investigated the protective effects of PEP-1-HSP27 against cell death and ischemic insults. When PEP-1-HSP27 fusion protein was added to the culture medium of astrocyte and primary neuronal cells, it rapidly entered the cells and protected them against cell death induced by oxidative stress. Immunohistochemical analysis revealed that, when PEP-1-HSP27 fusion protein was intraperitoneally injected into gerbils, it prevented neuronal cell death in the CA1 region of the hippocampus in response to transient forebrain ischemia. Our results demonstrate that transduced PEP-1-HSP27 protects against cell death in vitro and in vivo, and suggest that transduction of PEP-1-HSP27 fusion protein provides a potential strategy for therapeutic delivery in various human diseases in which reactive oxygen species are implicated, including stroke.
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chunchon, Korea.
authors.forename: Jae J
authors.initials: JJ
authors.lastname: An
authors.forename: Yeom P
authors.initials: YP
authors.lastname: Lee
authors.forename: So Y
authors.initials: SY
authors.lastname: Kim
authors.forename: Sun H
authors.initials: SH
authors.lastname: Lee
authors.forename: Min J
authors.initials: MJ
authors.lastname: Lee
authors.forename: Min S
authors.initials: MS
authors.lastname: Jeong
authors.forename: Dae W
authors.initials: DW
authors.lastname: Kim
authors.forename: Sang H
authors.initials: SH
authors.lastname: Jang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Moo H
authors.initials: MH
authors.lastname: Won
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Kil S
authors.initials: KS
authors.lastname: Lee
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Won S
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Y
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects; D020520:Brain Infarction / Q000517:prevention & control*; D016923:Cell Death; D002470:Cell Survival; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D005822:Genetic Vectors / Q000235:genetics; D005849:Gerbillinae; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D006624:Hippocampus; D006801:Humans; D015227:Lipid Peroxidation; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D009474:Neurons / Q000187:drug effects; D018384:Oxidative Stress; D010455:Peptides / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000494:pharmacology / Q000627:therapeutic use*; D014161:Transduction, Genetic
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; C503924:Pep-1 peptide; D010455:Peptides; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D003543:Cysteamine