Defences against oxidative stress in vibrios associated with corals.
Bacteria colonizing healthy coral tissue may produce enzymes capable of overcoming the toxic effects of reactive oxygen species, including superoxide dismutase (SOD) and catalase. Significant differences in the activities of these enzymes were observed in cultures of Vibrio campbellii, Vibrio coralliilyticus, Vibrio harveyi, Vibrio mediterranei, Vibrio pelagius, Vibrio rotiferanus, Vibrio tasmaniensis, and Photobacterium eurosenbergii isolated from healthy, bleached or necrotic tropical and cold water corals. Levels of SOD in exponential phase cultures of V. coralliilyticus grown at 28 degrees C were only slightly higher than those grown at 16 degrees C whereas the levels in stationary phase cultures at 28 degrees C were 7.3 x higher than those at 16 degrees C. The increase in catalase activity of V. coralliilyticus and V. harveyi upon entry to stationary phase conferred protection against killing by oxidative stress. Increased temperature affected up-regulation of enzymes in stationary phase cultures, but pretreatment of cultures with hydrogen peroxide had no significant effect on induction of catalase or SOD. The increased activities appear to be due to up-regulation of gene expression rather than induction of different forms of the enzymes. We suggest that SOD and catalase are unlikely to be major factors in the virulence of these bacteria for corals and that their main function may be to protect against endogenous superoxide.
Munn
Colin B
CB
School of Biological Sciences, University of Plymouth, Plymouth, UK. cmunn@plymouth.ac.uk
Marchant
Hannah K
HK
Moody
A John
AJ
D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D002374:Catalase / Q000378:metabolism; D015169:Colony Count, Microbial; D015964:Gene Expression Regulation, Bacterial; D006861:Hydrogen Peroxide / Q000633:toxicity; D050296:Microbial Viability; D018384:Oxidative Stress; D010100:Oxygen / Q000633:toxicity*; D010776:Photobacterium / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D015854:Up-Regulation; D014733:Vibrio / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification
D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen