title: Generation of a conditional null allele for Dmp1 in mouse.
abstract: Dentin matrix protein1 (DMP1), highly conserved in humans and mice, is highly expressed in teeth, the skeleton, and to a lesser extent in nonskeletal tissues such as brain, kidney, and salivary gland. Pathologically, DMP1 is associated with several forms of cancers and with tumor-induced osteomalacia. Conventional disruption of the murine Dmp1 gene results in defects in dentin in teeth and in the skeleton, including hypophosphatemic rickets, and abnormalities in phosphate homeostasis. Human DMP1 mutations are responsible for the condition known as autosomal recessive hypophosphatemic rickets. For better understanding of the roles of DMP1 in different tissues at different stages of development and in pathological conditions, we generated Dmp1 floxed mice in which loxP sites flank exon 6 that encodes for over 80% of DMP1 protein. We demonstrate that Cre-mediated recombination using Sox2-Cre, a Cre line expressed in epiblast during early embryogenesis, results in early deletion of the gene and protein. These homozygous Cre-recombined null mice display an identical phenotype to conventional null mice. This animal model will be useful to reveal distinct roles of DMP1 in different tissues at different ages.
authors.affiliation: Department of Biomedical Sciences, Baylor College of Dentistry, Texas A&M Health Science Center, Dallas, Texas 75246, USA. jfeng@bcd.tamhsc.edu
authors.forename: Jian Q
authors.initials: JQ
authors.lastname: Feng
authors.forename: Greg
authors.initials: G
authors.lastname: Scott
authors.forename: Dayong
authors.initials: D
authors.lastname: Guo
authors.forename: Baichun
authors.initials: B
authors.lastname: Jiang
authors.forename: Marie
authors.initials: M
authors.lastname: Harris
authors.forename: Toni
authors.initials: T
authors.lastname: Ward
authors.forename: Manas
authors.initials: M
authors.lastname: Ray
authors.forename: Lynda F
authors.initials: LF
authors.lastname: Bonewald
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Harris
authors.forename: Yuji
authors.initials: Y
authors.lastname: Mishina
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000196:embryology* / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005091:Exons; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D017353:Gene Deletion; D005821:Genetic Techniques; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic
chemical_list: C513308:Dmp1 protein, mouse; D016326:Extracellular Matrix Proteins; C045073:Cre recombinase; D019426:Integrases