title: Simultaneous determination of mycophenolic acid and its acyl and phenol glucuronide metabolites in human serum by capillary zone electrophoresis.
abstract: A simple capillary electrophoretic method was developed for simultaneous determination of mycophenolic acid (MPA) and its metabolites, acyl glucuronide (AcMPAG) and phenol glucuronide (MPAG), in human serum. The method utilized only running buffer in the separation, prior acidification of serum, and extraction with ethyl acetate. Separation was performed by capillary zone electrophoresis using 20 mM sodium acetate-acetic acid (pH 4.9) as running buffer, applied voltage of 15 kV, and UV detection at 217 nm. Each electrophoretic run was completed within 14 min. The optimized method demonstrated good performance concerning specificity, linearity (r>0.998), sensitivity (limit of detection: for MPA, 0.10 microg/ml; for AcMPAG, 0.10 microg/ml; MPAG, 0.42 microg/ml), accuracy (87-108%) and precision (<9.3%). This method was successfully applied to measurements of MPA, AcMPAG, and MPAG in renal transplant patient samples and could be a useful alternative to HPLC-based methods.
authors.affiliation: Department of Hospital Pharmacy, Nagasaki University Hospital of Medicine and Dentistry, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. k-ohyama@nagasaki-u.ac.jp
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Hiroo
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
authors.forename: Masaharu
authors.initials: M
authors.lastname: Nishikido
authors.forename: Mugen
authors.initials: M
authors.lastname: Teshima
authors.forename: Hideto
authors.initials: H
authors.lastname: To
authors.forename: Takashi
authors.initials: T
authors.lastname: Kitahara
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005965:Glucuronates / Q000097:blood*; D006801:Humans; D009173:Mycophenolic Acid / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D005965:Glucuronates; D009173:Mycophenolic Acid