title: The 3-hydroxyacyl-ACP dehydratase of mitochondrial fatty acid synthesis in Trypanosoma brucei.
abstract: The trypanosomatid parasite Trypanosoma brucei synthesizes fatty acids in the mitochondrion using the type II fatty acid synthesis (FAS) machinery. When mitochondrial FAS was characterized in T. brucei, all of the enzymatic components were identified based on their homology to yeast mitochondrial FAS enzymes, except for 3-hydroxyacyl-ACP dehydratase. Here we describe the characterization of T. brucei mitochondrial 3-hydroxyacyl-ACP dehydratase (TbHTD2), which was identified by its similarity to the human mitochondrial dehydratase. TbHTD2 can rescue the respiratory deficient phenotype of the yeast knock-out strain and restore the lipoic acid content, is localized in the mitochondrion and exhibits hydratase 2 activity.
authors.affiliation: Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland. kaija.autio@oulu.fi
authors.forename: Kaija J
authors.initials: KJ
authors.lastname: Autio
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Guler
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Kastaniotis
authors.forename: Paul T
authors.initials: PT
authors.lastname: Englund
authors.forename: J Kalervo
authors.initials: JK
authors.lastname: Hiltunen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004586:Electrophoresis; D005227:Fatty Acids / Q000096:biosynthesis*; D005816:Genetic Complementation Test; D006801:Humans; D006836:Hydro-Lyases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism; D016415:Sequence Alignment; D008063:Thioctic Acid / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000201:enzymology*
chemical_list: D005227:Fatty Acids; D011993:Recombinant Fusion Proteins; D008063:Thioctic Acid; D006836:Hydro-Lyases