Minipreps of plasmid DNA.
Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.
Engebrecht
J
J
State University of New York, Stony Brook, New York, USA.
Brent
R
R
Kaderbhai
M A
MA
D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D005822:Genetic Vectors / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods*; D010957:Plasmids / Q000302:isolation & purification*
D004247:DNA