Expression of Etk/Bmx tyrosine kinase in intrahepatic cholangiocarcinoma.
BACKGROUND
Epithelial and endothelial tyrosine kinase (Etk), also known as bone marrow X kinase (Bmx) plays an important role in the growth, differentiation, apoptosis, proliferation, and tumorigenicity of epithelial cells. The purpose of this study was to investigate the expression of Etk/Bmx in intrahepatic cholangiocarcinoma (ICC) and correlated the expression with clinicopathological parameters.


METHODS
Fifty-seven cases of ICC were immunostained for Etk/Bmx, HCV NS(5), PCNA, bcl-2, and NF-kappa B p65.


RESULTS
Etk/Bmx expression was present in 19 (33.3%) of 57 ICC specimens and correlated with cell differentiation and survival rate but not closely with tumor size and lymph node metastasis. There was a significant difference of expression of either PCNA-LI or Bcl-2 between Etk/Bmx-positive and -negative cases (P < 0.05). However, no statistically significant association was found between Etk/Bmx expression and presence of HCV-NS5 or NF-kappa B p65.


CONCLUSIONS
Our results indicate that Etk/Bmx may be involved in the development of ICC and be a predictor of poor prognosis for ICC. HCV infection and NF-kappa B appears unrelated to Etk/Bmx expression.
Guo
Linlang
L
Department of Pathology, Zhujiang Hospital, NanFang Medical University, Guangzhou, China.
Guo
Ying
Y
Xiao
Sha
S
D001650:Bile Duct Neoplasms / Q000378:metabolism* / Q000401:mortality; D001653:Bile Ducts, Intrahepatic; D002454:Cell Differentiation; D018281:Cholangiocarcinoma / Q000378:metabolism* / Q000401:mortality; D019254:Genes, bcl-2; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D016019:Survival Analysis; D051996:Transcription Factor RelA / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase
D018809:Proliferating Cell Nuclear Antigen; D011505:Protein-Tyrosine Kinases; D051996:Transcription Factor RelA; D017361:Viral Nonstructural Proteins; C492367:BMX protein, human; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase