IgG deposition and activation of the classical complement pathway involvement in the activation of human granulocytes by decellularized porcine heart valve tissue.
Decellularization treatment of heart valves has been thought to eliminate tissue immunogenicity. Early failure of tissue-engineered xenogeneic heart valves was seen in children and has been a major drawback in this promising field of research. This study was designed to characterize the effects of acellular porcine heart valve tissue on immune activation in vitro. Incubation of decellularized porcine tissue with human plasma led to adsorption of IgG, activation of the classical complement pathway and adhesion of activated polymorphonuclear leukocytes (PMN). This inflammatory response was strongly inhibited by proteins extracted from native porcine tissue which might indicate that inhibitors of PMN activation present in the extracellular matrix (ECM) are lost during the decellularization process.
Bastian
Fabienne
F
Department of Cardiothoracic Surgery, Medical University Vienna, Vienna, Austria.
Stelzmüller
Marie-Elisabeth
ME
Kratochwill
Klaus
K
Kasimir
Marie-Theres
MT
Simon
Paul
P
Weigel
Guenter
G
D000368:Aged; D000818:Animals; D002474:Cell-Free System / Q000276:immunology; D002478:Cells, Cultured; D003167:Complement Activation / Q000276:immunology*; D003171:Complement Pathway, Classical / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006351:Heart Valves / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008297:Male; D013552:Swine
D007074:Immunoglobulin G