title: Temporal evolution of soluble Fas and Fas ligand in patients with orthotopic liver transplantation.
abstract: OBJECTIVE
  The aim of this study was to analyze the expression levels of plasma soluble Fas (sFas) and soluble Fas ligand (sFasL) in patients with orthotopic liver transplantation (OLT) procedures routinely performed without venovenous bypass.
  
  
  METHODS
  The sFas and sFasL were analyzed in the blood of 20 consecutive patients who underwent transplantation. Blood samples were drawn from the radial artery at serial time points before, during, and after surgery. Plasma levels of sFas and sFasL were detected by Enzyme Linked-Immuno-Sorbent Assay. Plasma aspartate transaminase (AST) and alanine transaminase (ALT) were assayed by routine clinical chemistry testing.
  
  
  RESULTS
  Marked elevation of plasma AST and ALT were detected at the reperfusion and postoperation time points (P<0.001), with a peak on the first postoperative day. The mean plasma concentration of sFas and sFasL remained unchanged from preoperative to anhepatic phase (T1 to T3) (P> or =0.268). The sFas and sFasL concentrations were significantly higher at 15 and 60 min after reperfusion compared to the preoperative value (P< or =0.048). Postoperatively, sFas and sFasL concentration were decreased to preoperative levels on the first postoperative day (P> or =0.127).
  
  
  CONCLUSIONS
  The sFas and sFasL seem to be involved in reperfusion injury during OLT. The understanding of Fas may provide new insights into the mechanisms of ischemia/reperfusion injury during OLT.
authors.affiliation: Orient Organ Transplant Center, Tianjin First Central Hospital, Nankai District, Fukang Road, No. 24, 300192 Tianjin, PR China. wang_yu_l@yahoo.com.cn
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yawu
authors.initials: Y
authors.lastname: Liu
authors.forename: Ruifa
authors.initials: R
authors.lastname: Han
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Shen
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D053222:Fas Ligand Protein / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D015427:Reperfusion Injury / Q000175:diagnosis / Q000209:etiology*; D019014:fas Receptor / Q000097:blood / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase