title: Alveolar bone regeneration using absorbable poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblast growth factor.
abstract: The aim of this study was to evaluate the effects of combining a porous poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblastic growth factor (bFGF) on bone regeneration in mandibular ridges. Four full-thickness saddle-type defects (10 mm long x 5 mm deep) were symmetrically created in both edentulous mandibular alveolar ridges of 6 beagles. The dome-shaped membrane was secured to each defect site, and a gelatin sponge containing 200 microg bFGF was implanted on the left side of each defect (experimental group). Only the membranes (control group) were secured to the defect sites on the right. Three and 6 months later, 3 animals were killed. Bone regeneration was analyzed by soft X-ray photographs, micro-computed tomography (CT) images, and peripheral quantitative CT (pQCT), and then examined histologically. Soft X-ray examination revealed an increase in new bone volume in the experimental group 6 months postoperatively. pQCT showed that immature bone density was higher in the experimental group. Micro-CT images revealed well formed new bone along the original contour of the dome-shaped membrane in the experimental group. Histologically, inflammatory infiltration of tissue surrounding the membranes was slight. These results suggest that combining the poly(L-lactide-co-epsilon-caprolactone)/beta-tricalcium phosphate membrane and bFGF-gelatin sponge is promising for alveolar ridge reconstruction.
authors.affiliation: Institute for Frontier Oral Science, Kanagawa Dental College, Yokosuka, Kanagawa, Japan. kinoshit@kdcnet.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kinoshita
authors.forename: M
authors.initials: M
authors.lastname: Matsuo
authors.forename: K
authors.initials: K
authors.lastname: Todoki
authors.forename: S
authors.initials: S
authors.lastname: Ozono
authors.forename: S
authors.initials: S
authors.lastname: Fukuoka
authors.forename: H
authors.initials: H
authors.lastname: Tsuzuki
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Tomihata
authors.forename: T
authors.initials: T
authors.lastname: Shimamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Ikada
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D000539:Alveolar Process / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D015519:Bone Density / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology*; D002130:Calcium Phosphates / Q000737:chemistry; D004285:Dogs; D016222:Fibroblast Growth Factor 2 / Q000627:therapeutic use*; D005781:Gelatin Sponge, Absorbable / Q000627:therapeutic use*; D048091:Guided Tissue Regeneration / Q000379:methods; D006490:Hemostatics / Q000627:therapeutic use*; D007575:Jaw, Edentulous / Q000503:physiopathology / Q000601:surgery; D008334:Mandible / Q000473:pathology / Q000503:physiopathology; D008336:Mandibular Diseases / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000502:physiology; D011091:Polyesters / Q000737:chemistry; D013526:Surgical Mesh; D014057:Tomography, X-Ray Computed / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D006490:Hemostatics; D008567:Membranes, Artificial; D011091:Polyesters; C471130:beta-tricalcium phosphate-poly-L-lactide copolymer; C523703:poly(L-lactide-co-glycolide-co-epsilon-caprolactone); D016222:Fibroblast Growth Factor 2