title: Comparison of nanoscale and microscale bioactive glass on the properties of P(3HB)/Bioglass composites.
abstract: This study compares the effects of introducing micro (m-BG) and nanoscale (n-BG) bioactive glass particles on the various properties (thermal, mechanical and microstructural) of poly(3hydroxybutyrate) (P(3HB))/bioactive glass composite systems. P(3HB)/bioactive glass composite films with three different concentrations of m-BG and n-BG (10, 20 and 30 wt%, respectively) were prepared by a solvent casting technique. The addition of n-BG particles had a significant stiffening effect on the composites, modulus when compared with m-BG. However, there were no significant differences in the thermal properties of the composites due to the addition of n-BG and m-BG particles. The systematic addition of n-BG particles induced a nanostructured topography on the surface of the composites, which was not visible by SEM in m-BG composites. This surface effect induced by n-BG particles considerably improved the total protein adsorption on the n-BG composites compared to the unfilled polymer and the m-BG composites. A short term in vitro degradation (30 days) study in simulated body fluid (SBF) showed a high level of bioactivity as well as higher water absorption for the P(3HB)/n-BG composites. Furthermore, a cell proliferation study using MG-63 cells demonstrated the good biocompatibility of both types of P(3HB)/bioactive glass composite systems. The results of this investigation confirm that the addition of nanosized bioactive glass particles had a more significant effect on the mechanical and structural properties of a composite system in comparison with microparticles, as well as enhancing protein adsorption, two desirable effects for the application of the composites in tissue engineering.
authors.affiliation: Department of Materials, Imperial College London, London, UK.
authors.forename: Superb K
authors.initials: SK
authors.lastname: Misra
authors.forename: Dirk
authors.initials: D
authors.lastname: Mohn
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Brunner
authors.forename: Wendelin J
authors.initials: WJ
authors.lastname: Stark
authors.forename: Sheryl E
authors.initials: SE
authors.lastname: Philip
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
authors.forename: Vehid
authors.initials: V
authors.lastname: Salih
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Knowles
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
mesh_terms: D001826:Body Fluids / Q000737:chemistry; D002460:Cell Line; D002516:Ceramics; D020770:Compomers / Q000737:chemistry / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000494:pharmacology*; D008420:Manufactured Materials; D008422:Materials Testing; D008863:Microspheres; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties; D000094122:Polyhydroxybutyrates
chemical_list: D020770:Compomers; D006885:Hydroxybutyrates; D011091:Polyesters; C425330:bioactive glass 45S5; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates