DNA-independent RNA polymerases.
Poly(A) polymerase, purified from E. coli, catalyzes the incorporation of AMP residues onto the free 3'-hydroxyl terminus of RNA, utilizing ATP as a precursor. This unit describes specific reaction conditions as well as some applications including labeling the 3' ends of RNA with alpha32ATP for hybridization probes, and cloning RNA that lacks a poly(A) tail. The poly(A) tail is synthesized by poly(A) polymerase, and cDNA is synthesized from the resulting RNA using oligo(dT) as a primer.
Tabor
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Harvard Medical School, Boston, Massachusetts, USA.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D009693:Nucleic Acid Hybridization
D012321:DNA-Directed RNA Polymerases