title: Silanizing glassware.
abstract: This unit presents a procedure to silanize (siliconize) glassware to prevent adsorption of solute to the glass surface or to increase its hydrophobicity. This is particularly important when dealing with low concentrations of particularly "sticky" solutes such as single-stranded nucleic acids or proteins.
authors.affiliation: Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: B
authors.initials: B
authors.lastname: Seed
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008967:Molecular Biology / Q000379:methods*; D011506:Proteins / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D011506:Proteins; D012821:Silanes