[Intergenus natural genetic transformation between Escherichia coli and Bacillus subtilis at different growth phase].
The culture fluids of Escheriachia coli with shuttle plasmid and Bacillus subtilis strains were mixed and coincubated for 40 minutes after culturing respectively in LB and minimal media. The steadily plasmid transfer by natural genetic transformation between these two gram-negative and gram-positive bacteria has been demonstrated by the methods of selective medium screening, DNase I sensitivity test and plasmid detection. In contrast to MM culture B. subtilis LB culture can be competent and has equivalent transformation frequency. Furthermore, the maximal transformation frequency was obtained when cells in exponential phase served as donors or recipients. It is suggested that B. subtilis solid transformation is different from liquid plasmid transformation including the whole process of DNA plasmid competence producing. Understanding the mechanisms of gene transfer between bacteria may aid in assessing the potential risk associated with the release of recombinant organisms into the environment.
Wang
Xiao-Juan
XJ
College of Life Sciences, Wuhan University, Wuhan 430072, China. xiaoheyuou80@yahoo.com.cn
Li
Mei-Ju
MJ
Chen
Xiang-Dong
XD
Xie
Zhi-Xiong
ZX
Shen
Ping
P
D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development; D003850:Deoxyribonuclease I / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014169:Transformation, Bacterial
D003850:Deoxyribonuclease I