Mouse embryonic stem (ES) cell isolation.
Building on the technology presented in earlier units of Chapter 23, this unit describes the basic method for the isolation of mouse embryonic (ES) stem cells. ES cells from wild-type mice may be used for the production of mouse mutants by homologous recombination and blastocyst-mediated transgenesis. ES cells from mutant mouse lines may be used in the analysis of mutant phenotypes.
Conner
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Harvard Medical School, Boston, Massachusetts, USA.
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D003470:Culture Media; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D005780:Gelatin; D007202:Indicators and Reagents; D051379:Mice; D013234:Stem Cells / Q000166:cytology*
D003470:Culture Media; D007202:Indicators and Reagents; D005780:Gelatin