title: Basophil sensitivity in patients not responding to venom immunotherapy.
abstract: BACKGROUND
  Treatment failure of venom immunotherapy (VIT) is not rare and the risk and pathogenic factors for those failures are so far poorly understood. For that reason we evaluated allergen-specific basophil sensitivity in patients who did not tolerate field re-stings after completed VIT treatment.
  
  
  METHODS
  Basophil responsiveness was evaluated by flow cytometry analyses of basophil CD63 surface expression induced by different concentrations of bee or wasp venom (1, 0.1 and 0.01 microg/ml) in 14 treated patients who had experienced systemic allergic reactions (Muller grades II-III) and 17 treated patients who had no reactions after the field re-stings. We also included a group of 28 Hymenoptera venom-allergic patients who had not received VIT.
  
  
  RESULTS
  In 14 patients who still reacted to bee or wasp sting, basophil response at a venom concentration of 0.1 microg/ml was significantly higher than in patients who tolerated field re-stings (p = 0.03; t test). Basophil response was also slightly higher at a concentration of 1 microg/ml, but not to statistical significance (p = 0.12; t test). There was no difference in the response to direct cross-linking of the IgE and in venom-specific IgE and IgG4 serum concentrations between those 2 groups (p > 0.8; Fisher's exact test, t test). Patients who tolerated field re-stings have also significantly lower basophil response in comparison to patients who had not received VIT, both at 0.1 and 1 microg/ml of venom concentrations (p < 0.001; t test).
  
  
  CONCLUSIONS
  The results suggest that basophil venom-specific sensitivity is associated with the efficiency of VIT.
authors.affiliation: University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia. andreja.mr_peternelj@klinika-golnik.si
authors.forename: Andreja
authors.initials: A
authors.lastname: Peternelj
authors.forename: Mira
authors.initials: M
authors.lastname: Silar
authors.forename: Renato
authors.initials: R
authors.lastname: Erzen
authors.forename: Mitja
authors.initials: M
authors.lastname: Kosnik
authors.forename: Peter
authors.initials: P
authors.lastname: Korosec
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015703:Antigens, CD / Q000097:blood / Q000276:immunology*; D001180:Arthropod Venoms / Q000276:immunology / Q000627:therapeutic use*; D001491:Basophils / Q000276:immunology*; D003888:Desensitization, Immunologic / Q000379:methods*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006927:Hymenoptera / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007299:Insect Bites and Stings / Q000276:immunology* / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D018709:Statistics, Nonparametric; D060149:Tetraspanin 30
chemical_list: D015703:Antigens, CD; D001180:Arthropod Venoms; C554586:CD63 protein, human; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D007073:Immunoglobulin E