Detection of hybridized probe.
Autoradiography is used to detect and quantitate radioactive probe hybridized to cytological preparations. Autoradiographic film is used to detect (32)P- or (35)S-labeled probe, and can be useful in experiments dealing with large organs or tissues. Emulsion autoradiography is required to obtain resolution at the level of a single cell. Both procedures are described here.
Rogers
M
M
Harvard Medical School and Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
D001345:Autoradiography; D015342:DNA Probes / Q000032:analysis* / Q000235:genetics*; D004655:Emulsions; D009693:Nucleic Acid Hybridization / Q000379:methods*
D015342:DNA Probes; D004655:Emulsions