title: Development of a reference material using methamphetamine abusers' hair samples for the determination of methamphetamine and amphetamine in hair.
abstract: In the present study, we developed a reference material (RM) using authentic hair samples for the determination of methamphetamine (MA) and its main metabolite, amphetamine (AP) in human hair. MA abusers' hair samples were collected, homogenized and finally bottled. The concentration of each bottle was determined using two extraction methods, agitation with 1% HCl in methanol at 38 degrees C and ultrasonication with methanol/5M HCl (20:1), followed by gas chromatography/mass spectrometry (GC-MS) after derivatization with trifluoroacetic anhydride (TFAA). Both analytical procedures were fully validated and their extraction efficiency was compared. The homogeneity of analytes was evaluated and their property values were determined with their uncertainties. The two methods were acceptable to analyze MA and AP in human hair through the validation and comparative studies using spiked and authentic hair samples as well as NIST SRM 2379 certified reference material. Satisfying homogeneity was reached for MA and AP in the prepared RM. Finally, a human hair RM containing MA and AP is prepared at the level of 7.64+/-1.24 and 0.54+/-0.07 ng/mg, respectively. This material can be useful in forensic laboratories for internal quality control and external quality assurance.
authors.affiliation: National Institute of Scientific Investigation, 331-1 Sinwol-7-dong, Yangcheon-gu, Seoul 158-707, Republic of Korea. spp26625@yahoo.co.kr
authors.forename: Sooyeun
authors.initials: S
authors.lastname: Lee
authors.forename: Yonghoon
authors.initials: Y
authors.lastname: Park
authors.forename: Wonkyung
authors.initials: W
authors.lastname: Yang
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Han
authors.forename: Sanggil
authors.initials: S
authors.lastname: Choe
authors.forename: Sangwhan
authors.initials: S
authors.lastname: In
authors.forename: Miae
authors.initials: M
authors.lastname: Lim
authors.forename: Heesun
authors.initials: H
authors.lastname: Chung
mesh_terms: D000661:Amphetamine / Q000032:analysis* / Q000378:metabolism; D019969:Amphetamine-Related Disorders / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008694:Methamphetamine / Q000032:analysis* / Q000378:metabolism; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D008694:Methamphetamine; D000661:Amphetamine