title: Cyst formation and activation of the extracellular regulated kinase pathway after kidney specific inactivation of Pkd1.
abstract: Polycystic kidney disease (ADPKD) results from failure of the kidney to properly maintain three-dimensional structure after loss of either polycystin-1 or -2. Mice with kidney selective inactivation of Pkd1 during embryogenesis develop profound renal cystic disease and die from renal failure within 3 weeks of birth. In this model, cysts form exclusively from cells in which Cre recombinase is active, but the apparent pace of cyst expansion varies by segment and cell type. Intercalated cells do not participate in cyst expansion despite the presence of cilia up to at least postnatal day 21. Cystic segments show a persistent increase in proliferation as determined by bromodeoxyuridine (BrdU) incorporation; however, the absolute proliferative index is dependent on the underlying proliferative potential of kidney tubule cells. Components of the extracellular regulated kinase (MAPK/ERK) pathway from Ras through MEK1/2 and ERK1/2 to the effector P90(RSK) are activated in both perinatal Pkd1 and adult Pkd2 ortholgous gene disease models. The pattern of MAPK/ERK activation is focal and does not correlate with the pattern of active proliferation identified by BrdU uptake. The possibility of a causal relationship between ERK1/2 activation and cyst cell proliferation was assessed in vivo in the acute perinatal Pkd1 model of ADPKD using MEK1/2 inhibitor U0126. U0126 treatment had no effect on progression of cyst formation in this model at doses sufficient to reduce phospho-ERK1/2 in cystic kidneys. Cysts in ADPKD exhibit both increased proliferation and activation of MAPK/ERK, but cyst growth is not prevented by inhibition of ERK1/2 activation.
authors.affiliation: Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Sekiya
authors.initials: S
authors.lastname: Shibazaki
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Yu
authors.forename: Saori
authors.initials: S
authors.lastname: Nishio
authors.forename: Xin
authors.initials: X
authors.lastname: Tian
authors.forename: R Brent
authors.initials: RB
authors.lastname: Thomson
authors.forename: Michihiro
authors.initials: M
authors.lastname: Mitobe
authors.forename: Angeliki
authors.initials: A
authors.lastname: Louvi
authors.forename: Heino
authors.initials: H
authors.lastname: Velazquez
authors.forename: Shuta
authors.initials: S
authors.lastname: Ishibe
authors.forename: Lloyd G
authors.initials: LG
authors.lastname: Cantley
authors.forename: Peter
authors.initials: P
authors.lastname: Igarashi
authors.forename: Stefan
authors.initials: S
authors.lastname: Somlo
mesh_terms: D000818:Animals; D017209:Apoptosis; D002070:Butadienes / Q000494:pharmacology; D049109:Cell Proliferation; D003560:Cysts / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D004195:Disease Models, Animal; D004789:Enzyme Activation; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D048369:MAP Kinase Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D048370:MAP Kinase Kinase 2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D016891:Polycystic Kidney, Autosomal Dominant / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D050396:TRPP Cation Channels
chemical_list: D002070:Butadienes; D048369:MAP Kinase Kinase 1; D048370:MAP Kinase Kinase 2; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D050396:TRPP Cation Channels; C113580:U 0126; C482129:Map2k2 protein, mouse