New protein clustering of breast cancer tissue proteomics using actin content as a cellularity indicator.
In the present study, we report the comparative proteome profiles of proteins solubilized from 37 breast cancer surgical tissues, normalized for the actin content. Blood-derived proteins were excluded from the analysis. Among the tumor-derived protein spots, a large proportion (39%) was found present in all patients. These included several glycolytic enzymes, detox and heat shock proteins, members of annexin and S100 protein families, cathepsin D, and two "rare" proteins, DDAH2 involved in the angiogenesis control, and the oncogene PARK7. Other proteins, such as psoriasin, galectin1, cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression level, which suggests their possible role for patient stratification.
Pucci-Minafra
Ida
I
Dipartimento di Oncologia Sperimentale e Applicazioni Cliniche (DOSAC), Università di Palermo, Patermo, Italy. idapucci@unipa.it
Cancemi
Patrizia
P
Albanese
Nadia Ninfa
NN
Di Cara
Gianluca
G
Marabeti
Maria Rita
MR
Marrazzo
Antonio
A
Minafra
Salvatore
S
D000199:Actins / Q000032:analysis*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism*; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D010449:Peptide Mapping; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000199:Actins; D020543:Proteome