[Detection of various component in the trace sample of gingival crevicular fluid].
OBJECTIVE
To evaluate methods of detecting various component in the trace sample of the gingival crevicular fluid (GCF).


METHODS
One sample of GCF was collected from four patients with mild or moderate periodontitis. At first, one half (group A)of every sample was measured for interleukin-6 (IL-6) by enzyme-linked immunosorbent assay (ELISA) and then detected for Interleukin-1beta (IL-1beta) in the same sample .Another half (group B) of the sample was used as control for the measurement of Interleukin-1beta (IL-1beta). Another sample of GCF was collected from nine patients with abutment and non-abutment because of the distal extension teeth lost resulted from the chronic periodontitis. At first, one half (group C) of the sample was detected for tumor necrosis factor-alpha (TNF-alpha ) by ELISA and then measured for elastase in the same sample. Another half (group D) of the sample was used as control for the measurement of elastase.


RESULTS
There was no significant difference of both the measured value of IL-1beta absorbency (group A and B are 0.5 +/- 0.4 and 0.5 +/- 0.4, respectively, P=0.136)and that of elastase absorbency (group C and D are 1.1 +/- 0.6 and 1.1 +/- 0.6, respectively, P=0.943) between the original elution and the sample after detection of IL-6 or TNF-alpha in the test 1 and test 2, respectively. Moreover, the values showed high correlation (r=0.993, P=0.000 in the group A and B; r=0.979, P=0.000 in the group C and D).


CONCLUSIONS
It is practicable that one sample of GCF can be reused to examine for several components of GCF.
Chen
Zhi-Bin
ZB
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Sun
Xiao-Jun
XJ
Kou
Chuan-Zhe
CZ
D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D008297:Male; D010196:Pancreatic Elastase / Q000032:analysis*
D007375:Interleukin-1; D010196:Pancreatic Elastase