Inhibitory effect of Agaricu blazei Murill components on abnormal collagen fiber formation in human hepatocarcinoma cells.
Hot-water extracts of the mycelial culture and fruiting bodies of Agaricus blazei Murill were fractionated by ethanol precipitation, using various ethanol concentrations. The mycelial fraction (A-4) inhibited abnormal collagen fiber formation, and fractions A-1 to A-3 showed a small inhibitory effect. The strongest inhibition was obtained by fraction A-4, and no significant inhibition was observed with fractions A-5 and A-6. With the fruiting bodies, fractions B-1 to B-6 showed no inhibitory effects on collagen fiber formation in HCC. The reverse transcription-polymerase chain reaction (RT-PCR) demonstrates that Agaricus blazei mycelial fraction A-4 did not inhibit the type I, II or III procollagen gene expression.
Sorimachi
Kenji
K
Educational Support Center, Dokkyo Medical University, Mibu, Tochigi 321-0293, Japan. kenjis@dokkyomed.ac.jp
Akimoto
Kazumi
K
Koge
Takashi
T
D000364:Agaricus / Q000737:chemistry*; D045744:Cell Line, Tumor; D003094:Collagen / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*
D010936:Plant Extracts; D003094:Collagen