title: Human tyrosinase inhibitor in rum distillate wastewater.
abstract: An inhibitor of human tyrosinase activity in rum distillate wastewater was isolated and identified as (S)-(+)-imperanene (1). (S)-(+)-Imperanene significantly inhibited tyrosinase isolated from HMV-II cells (IC(50) 1.85 mM). Inhibition kinetics studies revealed that imperanene is a competitive inhibitor of tyrosinase when L-3,4-dihydroxyphenylalanine is used as the substrate. The inhibitory activities of 1, O-beta-D-glucopyranosyl imperanene (2) and O-beta-D-glucopyranosyl-3-methoxyl imperanene (3) were 1>2>3.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Takara
authors.forename: Hironori
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Ujihara
authors.forename: Koji
authors.initials: K
authors.lastname: Wada
mesh_terms: D000434:Alcoholic Beverages; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014866:Waste Products; D014867:Water / Q000032:analysis / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D014866:Waste Products; D014867:Water; D014442:Monophenol Monooxygenase