Activating effect of benzbromarone, a uricosuric drug, on peroxisome proliferator-activated receptors.
Benzbromarone, a uricosuric drug, reportedly causes hepatic hypertrophy accompanied by proliferation of peroxisomes in rats. To elucidate the mechanisms underlying induction of peroxisome proliferation by benzbromarone, we examined binding affinity for peroxisome proliferator-activated receptor alpha (PPARalpha) and gamma (PPARgamma), and effects on the binding activity of PPARs with peroxisome proliferation-responsive element (PPRE) and expression of the PPARs target protein. Binding affinity of benzbromarone for PPARalpha and PPARgamma was examined by reporter gene assay. Binding activity of PPARs with PPRE was determined by electric mobility shift assay, and expression of lipoprotein lipase (LPL) and acyl-CoA synthetase (ACS) by Western blot method. Benzbromarone displayed affinity for PPARalpha and PPARgamma, and promoted binding of PPARs to PPRE. Furthermore, cultured cells with benzbromarone added showed upregulated expression of LPL and ACS. These results suggest that benzbromarone induces peroxisome proliferation in hepatocytes by binding to PPARs, and controls expression of proteins related to lipid metabolism.
Kunishima
Chiyoko
C
Department of Pharmacovigilance, Torii Pharmaceutical Co., Ltd., 3-4-1 Nihonbashi-honcho, Chuo-ku, Tokyo 103-8439, Japan.
Inoue
Ikuo
I
Oikawa
Toshihiro
T
Nakajima
Hiromu
H
Komoda
Tsugikazu
T
Katayama
Shigehiro
S