title: Introduction of DNA into S. pombe cells.
abstract: Methods of transformation rely upon conditioning cells to take up DNA, and growing them under selective conditions to establish and maintain the plasmid or integration. Different methods may be used, including electroporation, treatment with lithium cations, or protoplast treatment which removes the cell wall. Protoplasts prepared as for transformation can also be induced to fuse with each other and undergo karyogamy, which provides a means of mating sterile strains.
authors.affiliation: The Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Susan L
authors.initials: SL
authors.lastname: Forsburg
mesh_terms: D000085:Acetates; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D018274:Electroporation / Q000379:methods; D007202:Indicators and Reagents; D008561:Membrane Fusion; D011523:Protoplasts / Q000166:cytology; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic
chemical_list: D000085:Acetates; D004271:DNA, Fungal; D004274:DNA, Recombinant; D007202:Indicators and Reagents; C488804:lithium acetate