[The study on the inueraction of FCLA with human serum albumin with spectroscopy techniques].
It is very important to detect reactive oxygen species (ROS) in biologic and medicinal research. FCLA, the Fluoresceinyl Cypridina Luciferin Analog, is a high sensitivity probe of detecting singlet oxygen and superoxide anions. The FCLA molecule can react with ROS and, after a series of reactions that result in the release of additional chemical energy, releases photons. Depending on the probe used, the photons are typically in visible wavelength range and easily measurable with conventional optical detectors. The binding reaction of FCLA with human serum albumin (HSA) was studied with spectroscopy techniques. It was found that HSA fluorescence was partially quenched by FCLA. The binding constants were obtained with quenching results. According to the Forster energy transfer mechanism, the energy efficiency and the distance between FCLA and HSA were calculated. Then it was found that static quenching was the main binding reaction between FCLA and HSA, and the energy transfer caused the HSA fluorescence quenching and the FCLA fluorescence producing. By studying the binding reaction of FCLA with HSA by spectroscopy techniques, this research proved the transfer style of FCLA and the interaction mechanism in the body. The results will offer some good advises about the ROS detection with the chemiluminesence probe in vivo. Meanwhile, it will also provide a new means to study pharmacokinetics and some basic theories about the medicine transfer in the body.
Zhou
Jing
J
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China.
Wei
Yan-Chun
YC
D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013816:Thermodynamics
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007093:Imidazoles; D012709:Serum Albumin; C080213:3,7-dihydro-6-(4-(2-(N'-(5-fluoresceinyl)thioureido)ethoxy)phenyl)-2-methylimidazo-(1,2-a)pyrazin-3-one