title: Experimental rat lung tumor model with intrabronchial tumor cell implantation.
abstract: OBJECTIVE
  The objective of this study was to develop a rat lung tumor model for anticancer drug testing.
  
  
  METHODS
  Sixty-two female Wistar rats weighing 208 +/- 20 g were anesthetized intraperitoneally with 2.5% tribromoethanol (1 ml/100 g live weight), tracheotomized and intubated with an ultrafine catheter for inoculation with Walker's tumor cells. In the first step of the experiment, a technique was established for intrabronchial implantation of 10(5) to 5 x 10(5) tumor cells, and the tumor take rate was determined. The second stage consisted of determining tumor volume, correlating findings from high-resolution computed tomography (HRCT) with findings from necropsia and determining time of survival.
  
  
  RESULTS
  The tumor take rate was 94.7% for implants with 4 x 10(5) tumor cells, HRCT and necropsia findings matched closely (r=0.953; p<0.0001), the median time of survival was 11 days, and surgical mortality was 4.8%.
  
  
  CONCLUSIONS
  The present rat lung tumor model was shown to be feasible: the take rate was high, surgical mortality was negligible and the procedure was simple to perform and easily reproduced. HRCT was found to be a highly accurate tool for tumor diagnosis, localization and measurement and may be recommended for monitoring tumor growth in this model.
authors.affiliation: Federal University of Ceará, Brazil. anterogn@terra.com.br
authors.forename: Antero
authors.initials: A
authors.lastname: Gomes Neto
authors.forename: Antônio Felipe Leite
authors.initials: AF
authors.lastname: Simão
authors.forename: Samuel de Paula
authors.initials: Sde P
authors.lastname: Miranda
authors.forename: Lívia Talita Cajaseiras
authors.initials: LT
authors.lastname: Mourão
authors.forename: Nilfácio Prado
authors.initials: NP
authors.lastname: Bezerra
authors.forename: Paulo Roberto Carvalho de
authors.initials: PR
authors.lastname: Almeida
authors.forename: Ronaldo de Albuquerque
authors.initials: Rde A
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D002279:Carcinoma 256, Walker / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D016014:Linear Models; D008175:Lung Neoplasms / Q000473:pathology* / Q000556:secondary; D051381:Rats; D014057:Tomography, X-Ray Computed / Q000379:methods; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods