Sister kinetochore recapture in fission yeast occurs by two distinct mechanisms, both requiring Dam1 and Klp2.
In eukaryotic cells, proper formation of the spindle is necessary for successful cell division. We have studied chromosome recapture in the fission yeast Schizosaccharomyces pombe. We show by live cell analysis that lost kinetochores interact laterally with intranuclear microtubules (INMs) and that both microtubule depolymerization (end-on pulling) and minus-end-directed movement (microtubule sliding) contribute to chromosome retrieval to the spindle pole body (SPB). We find that the minus-end-directed motor Klp2 colocalizes with the kinetochore during its transport to the SPB and contributes to the effectiveness of retrieval by affecting both end-on pulling and lateral sliding. Furthermore, we provide in vivo evidence that Dam1, a component of the DASH complex, also colocalizes with the kinetochore during its transport and is essential for its retrieval by either of these mechanisms. Finally, we find that the position of the unattached kinetochore correlates with the size and orientation of the INMs, suggesting that chromosome recapture may not be a random process.
Gachet
Yannick
Y
Laboratoire de Biologie Cellulaire et Moléculaire du Controle de la Prolifération (LBCMCP), Centre National de la Recherche Scientifique, Université de Toulouse, 31062 Toulouse, France.
Reyes
Céline
C
Courthéoux
Thibault
T
Goldstone
Sherilyn
S
Gay
Guillaume
G
Serrurier
Céline
C
Tournier
Sylvie
S
D001693:Biological Transport, Active; D004398:Dyneins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018386:Kinetochores / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000235:genetics / Q000378:metabolism; D046912:Multiprotein Complexes; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D012854:Sister Chromatid Exchange / Q000235:genetics / Q000502:physiology; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism
C485182:Cyan Fluorescent Protein; C527984:Dam1 protein, S pombe; C502171:Klp2 protein, S pombe; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D046912:Multiprotein Complexes; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; C505433:Dhc1 protein, S pombe; D004398:Dyneins