Improved T cell responses to Plasmodium falciparum circumsporozoite protein in mice and monkeys induced by a novel formulation of RTS,S vaccine antigen.
Protection against Plasmodium falciparum sporozoite infection can be achieved by vaccination with the recombinant circumsporozoite protein-based vaccine RTS,S formulated with the AS02A Adjuvant System. Since this protection is only partial and wanes over time, we have developed a new RTS,S-based vaccine adjuvanted with AS01B. RTS,S/AS01B-induced high specific antibody titers and increased the frequency of mouse CD4(+) and CD8(+) T cells expressing IFN-gamma, and of monkey CD4(+) T cells expressing IL-2 and/or IFN-gamma and/or TNF-alpha upon stimulation with vaccine antigens. Our data provides clear evidence that combining RTS,S antigen with a potent adjuvant induces strong humoral and cellular responses in vivo.
Mettens
Pascal
P
GlaxoSmithKline Biologicals, Rixensart, Belgium. Pascal.Mettens@gskbio.com
Dubois
Patrice M
PM
Demoitié
Marie-Ange
MA
Bayat
Babak
B
Donner
Marie-Noëlle
MN
Bourguignon
Patricia
P
Stewart
V Ann
VA
Heppner
D Gray
DG
Garçon
Nathalie
N
Cohen
Joe
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008253:Macaca mulatta; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010963:Plasmodium falciparum / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000913:Antibodies, Protozoan; D007376:Interleukin-2; D017780:Malaria Vaccines; C526041:RTS,S-AS01B vaccine; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma