title: Proteomic analysis of methamphetamine-induced reinforcement processes within the mesolimbic dopamine system.
abstract: ABSTRACT Methamphetamine (MAP) is a commonly used, addictive drug, and a powerful stimulant that dramatically affects the central nervous system. In this study, we used the conditioned place preference (CPP) paradigm in order to study the reinforcing properties of MAP and the herewith associated changes in proteins within the mesolimbic dopamine system. A CPP was induced by MAP after three intermittent intraperitoneal injections (1 mg/kg) in rats and protein profiles in the nucleus accumbens, striatum, prefrontal cortex, cingulate cortex and hippocampus were compared with a saline-treated control group. In addition, a group of animals was run through extinction and protein profiles were compared with a non-extinguished group. Protein screening was conducted using two-dimensional electrophoresis analysis which identified 27 proteins in the group that showed MAP-induced CPP. Some of the proteins were confirmed by Western lot analysis. Identified proteins had functions related to the cytoskeleton, transport/endocytosis or exocytosis (e.g. profilin-2 and syntaxin-binding protein), and signal transduction, among others.
authors.affiliation: Department of Biological Science, Sookmyung Women's University, Korea.
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Yang
authors.forename: Seyoon
authors.initials: S
authors.lastname: Kim
authors.forename: Min-Suk
authors.initials: MS
authors.lastname: Jung
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Shim
authors.forename: Na Kyung
authors.initials: NK
authors.lastname: Ryu
authors.forename: Yeon Joo
authors.initials: YJ
authors.lastname: Yook
authors.forename: Choon-Gon
authors.initials: CG
authors.lastname: Jang
authors.forename: Young Yil
authors.initials: YY
authors.lastname: Bahk
authors.forename: Kee-Won
authors.initials: KW
authors.lastname: Kim
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D000697:Central Nervous System Stimulants / Q000008:administration & dosage / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D008032:Limbic System / Q000187:drug effects*; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage / Q000494:pharmacology*; D009473:Neuronal Plasticity / Q000187:drug effects; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012054:Reinforcement, Psychology
chemical_list: D000697:Central Nervous System Stimulants; D008694:Methamphetamine; D004298:Dopamine