Potassium channel phosphorylation in excitable cells: providing dynamic functional variability to a diverse family of ion channels.
Phosphorylation of potassium channels affects their function and plays a major role in regulating cell physiology. Here, we review previous studies of potassium channel phosphorylation, focusing first on studies employing site-directed mutagenesis of recombinant channels expressed in heterologous cells. We then discuss recent mass spectrometric-based approaches to identify and quantify phosphorylation at specific sites on native and recombinant potassium channels, and newly developed mass spectrometric-based techniques that may prove beneficial to future studies of potassium channel phosphorylation, its regulation, and its mechanism of channel modulation.
Park
Kang-Sik
KS
Section of Neurobiology, Physiology and Behavior, College of Biological Sciences, Department of Physiology and Membrane Biology, School of Medicine, University of California, Davis, USA.
Yang
Jae-Won
JW
Seikel
Edward
E
Trimmer
James S
JS
D000818:Animals; D006801:Humans; D015640:Ion Channel Gating; D013058:Mass Spectrometry / Q000379:methods; D008564:Membrane Potentials; D008958:Models, Molecular; D009154:Mutation; D010766:Phosphorylation; D015221:Potassium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D013997:Time Factors
D015221:Potassium Channels; D011994:Recombinant Proteins