Rapid prediction and optimization of concentration conditions for preparative fractions by solid-phase extraction.
SPE is an effective tool for concentrating preparative fractions isolated from a complex sample. To guarantee high efficiency and recovery of concentration, the concentration conditions could be optimized by predicting the breakthrough volume (V(B)). In this study, a method of predicting V(B )of unknown compounds in preparative fractions at any isocratic mobile phase composition with the analytical retention parameters a and c is described. The a and c values and the relationship between half peak width (W(1/2)) and retention time of a model analyte were measured using the analytical elution mode on an SPE column, and the V(B )and retention volume (V(R)) predicted with the a and c values were validated with breakthrough experiments. However, it is impossible to measure the a and c values of multiple compounds in a complex system directly on an SPE column with a low number of theoretical plates. The correlation of the a and c values between the SPE and analytical columns was developed so that the analytical data could be transferred to the SPE column. With the calculated a and c values, we could optimize the concentration conditions on the basis of the predicted V(B )and the volume of the preparative fraction.
Jin
Yu
Y
Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Dalian, China.
Xue
Xing-ya
XY
Zhang
Fei-fang
FF
Zhang
Jing
J
Shi
Hui
H
Xiao
Yuan-sheng
YS
Ke
Yan-xiong
YX
Liang
Xin-miao
XM
D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D008956:Models, Chemical; D011237:Predictive Value of Tests; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization