Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.
Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long-term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free, recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.
Choi
Vivian W
VW
University of North Carolina, Chapel Hill, North Carolina, USA.
Asokan
Aravind
A
Haberman
Rebecca A
RA
Samulski
Richard Jude
RJ
D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods