[Determination of praziquantel residue in aquatic products using high performance liquid chromatography].
A sensitive and simple method for the determination of praziquantel residue in aquatic products has been established. The sample was extracted with ethyl acetate for three times (20 mL, 20 mL, 10 mL) and cleaned up with an LC-Si column. The extract was separated on a ZORBAX SB-C18 column (250 mm x 4.6 mm, 5 microm). Ultraviolet detection was performed at 214 nm. Acetonitrile-water (50: 50, v/v) was used as the mobile phase at a flow rate of 0.9 mL/min. The calibration curve of praziquantel in aquatic products at the concentration range of 0.02 - 20 mg/L was linear ( r = 0.999 98). The recovery of praziquantel was higher than 85%. The limit of detection was 10 microg/kg (S/N > 3).
Shen
Xiaosheng
X
The Fishery Products Quality Inspection and Test Center of Ministry of Agriculture, East China. foodsmc98@tom.com
Yu
Huijuan
H
Cai
Youqiong
Y
Huang
Dongmei
D
D000085:Acetates / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003445:Crustacea / Q000737:chemistry; D011223:Praziquantel / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D017210:Tilapia / Q000378:metabolism
D000085:Acetates; D011223:Praziquantel; C007650:ethyl acetate