Principles and application of fluorescence microscopy.
This overview discusses the principle of fluorescence along with practical discussions of fluorescent molecular probes, filters and filter sets, multiband filters and multi-dye fluorescence, light sources, objective lenses, image resolution and the point-spread function, fluorescence microscopy of living cells, and immunolabeling.
Coling
D
D
University of California Berkeley, Berkeley, California, USA.
Kachar
B
B
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002470:Cell Survival; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*
D000906:Antibodies; D005456:Fluorescent Dyes