title: RNA ligases.
abstract: T4 RNA ligase, the product of the phage gene 63, is purified from phage-infected cells. It catalyzes the ATP-dependent covalent joining of single-stranded 5'-phosphoryl termini of DNA or RNA to single-stranded 3'-hydroxyl termini of DNA or RNA. This unit describes specific reaction conditions as well as applications such as radioactive labeling of 3' termini of RNA, circularizing deoxy- and ribo-oligonucleotides, ligating oligomers for oligonucleotide synthesis, and stimulating the blunt-end ligation activity of T4 DNA ligase.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: S
authors.initials: S
authors.lastname: Tabor
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology*; D002384:Catalysis; D008025:Ligases / Q000378:metabolism*
chemical_list: D008025:Ligases