Endotoxemia does not limit energy supply in exercising rat skeletal muscle.
Although depletion in high-energy phosphorylated compounds and mitochondrial impairment have been reported in septic skeletal muscle at rest, their impact on energy metabolism has not been documented during exercise. In this study we aimed to investigate strictly gastrocnemius muscle function non-invasively, using magnetic resonance techniques in endotoxemic rats. Endotoxemia was induced by injecting animals intraperitoneally at t(0) and t(0) + 24 h with Klebsiella pneumoniae lipopolysaccharides (at 3 mg kg(-1)). Investigations were performed at t(0) + 48 h during a transcutaneous electrical stimulation protocol consisting of 5.7 min of repeated isometric contractions at a frequency of 3.3 HZ. Endotoxin treatment produced a depletion in basal phosphocreatine content and a pronounced reduction in oxidative adenosine triphosphate (ATP) synthesis capacity, whereas the resting ATP concentration remained unchanged. During the stimulation period, endotoxemia caused a decrease in force-generating capacity that was fully accounted for by the loss of muscle mass. It further induced an acceleration of glycolytic ATP production and an increased accumulation of adenosine diphosphate (ADP, an important mitochondrial regulator) that allowed a near-normal rate of oxidative ATP synthesis. Finally, endotoxemia did not affect the total rate of ATP production or the ATP cost of contraction throughout the whole stimulation period. These data demonstrate that, in an acute septic phase, metabolic alterations in resting muscle do not impact energy supply in exercising muscle, likely as a result of adaptive mechanisms.
Giannesini
Benoit
B
Centre de Résonance Magnétique Biologique et Médicale (CRMBM), UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France. benoit.giannesini@univmed.fr
Izquierdo
Marguerite
M
Dalmasso
Christiane
C
Le Fur
Yann
Y
Cozzone
Patrick J
PJ
Verleye
Marc
M
Le Guern
Marie-Emmanuelle
ME
Gillardin
Jean-Marie
JM
Bendahan
David
D
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D019446:Endotoxemia / Q000139:chemically induced / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000737:chemistry; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism*; D010766:Phosphorylation; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000139:chemically induced / Q000378:metabolism
D008070:Lipopolysaccharides; D000255:Adenosine Triphosphate