A comparative study of free and immobilized soybean and horseradish peroxidases for 4-chlorophenol removal: protective effects of immobilization.
Horseradish peroxidase (HRP) and soybean peroxidase (SBP) were covalently immobilized onto aldehyde glass through their amine groups. The activity yield and the protein content for the immobilized SBP were higher than for the immobilized HRP. When free and immobilized peroxidases were tested for their ability to remove 4-chlorophenol from aqueous solutions, the removal percentages were higher with immobilized HRP than with free HRP, whereas immobilized SBP needs more enzyme to reach the same conversion than free enzyme. In the present paper the two immobilized derivatives are compared. It was found that at an immobilized enzyme concentration in the reactor of 15 mg l(-1), SBP removed 5% more of 4-chlorophenol than HRP, and that a shorter treatment was necessary. Since immobilized SBP was less susceptible to inactivation than HRP and provided higher 4-chlorophenol elimination, this derivative was chosen for further inactivation studies. The protective effect of the immobilization against the enzyme inactivation by hydrogen peroxide was demonstrated.
Bódalo
Antonio
A
Chemical Engineering Department, University of Murcia, Campus de Espinardo, Murcia, Spain.
Bastida
Josefa
J
Máximo
M Fuensanta
MF
Montiel
M Claudia
MC
Gómez
María
M
Murcia
M Dolores
MD
D001671:Biochemistry / Q000379:methods*; D019149:Bioreactors; D002733:Chlorophenols / Q000737:chemistry*; D004789:Enzyme Activation; D004798:Enzymes / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005898:Glass; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009195:Peroxidase / Q000737:chemistry*; D010544:Peroxidases / Q000737:chemistry; D016062:Porosity; D013025:Glycine max / Q000201:enzymology*
D002733:Chlorophenols; D004798:Enzymes; D004800:Enzymes, Immobilized; C029107:4-chlorophenol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases; D009195:Peroxidase