[Consideration of the results of enzyme immunoassay in tissue helminthisms in antibody units].
Two hundred and twenty-two serum samples from patients infected with Toxocara, 374 from those infected with Echinococcus alveolaris, and 200 from healthy blood donors, which were screened in the enzyme immunoassay system, were used to assess an instrumental method for considering the assay results expressed in antibody units (AU), A direct correlation was found between the values of the intensity of an immune response in the infected, which were expressed in AU and titers routinely used for this purpose. Introducing the optic density index, standardized by the manufacturer of a reference test-positive sample kit, into the formula to calculate AU reduces possible variations in the assay results both between different batches and within one batch of kits.
Poletaeva
O G
OG
Starkova
T V
TV
Kovrova
E A
EA
Krasovskaia
N N
NN
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis* / Q000097:blood; D000941:Antigens; D000947:Antigens, Helminth; D003937:Diagnosis, Differential; D004443:Echinococcosis / Q000097:blood / Q000175:diagnosis*; D004446:Echinococcus / Q000276:immunology*; D023381:Endpoint Determination; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D014119:Toxocara / Q000276:immunology*; D014120:Toxocariasis / Q000097:blood / Q000175:diagnosis*
D000909:Antibodies, Helminth; D000941:Antigens; D000947:Antigens, Helminth