[The in vitro and in vivo experimental models of erectile nerve regeneration].
Neurogenic erectile dysfunction (NED) caused by pelvic floor surgeries/radiation therapies and associated with Parkinsons disease and diabetes remains a challenging healthcare issue. To facilitate NED research we have developed in vitro and in vivo experimental models. The in vitro model comprises the isolation, culture and treatment of rat major pelvic ganglia (MPG), which then produce outgrowing neurites whose length and molecular composition are indicative of the neurotrophic effect of the treatment agent. Through this approach we have confirmed that the brain-derived neurotrophic factor (BDNF) promotes nerve regeneration by activating the JAK/STAT signaling pathway. This has been further established by our in vivo model, which involves the transection or cruch of cavernous nerves and treatment with BDNF.
Lin
Gui-Ting
GT
Department of Urology, University of California, San Francisco, CA 94143, USA.
Lue
Tom F
TF
Lin
Ching-Shwun
CS
D000818:Animals; D004195:Disease Models, Animal; D007172:Erectile Dysfunction / Q000503:physiopathology*; D005724:Ganglia / Q000503:physiopathology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D009416:Nerve Regeneration; D010388:Pelvis / Q000294:innervation; D010413:Penis / Q000294:innervation / Q000503:physiopathology