title: Genetic diversity within and between broodstocks of the white shrimp Litopenaeus vannamei (Boone, 1931) (Decapoda, Penaeidae) and its implication for the gene pool conservation.
abstract: Genetic variation within and between fifteen closed broodstock lines of the Pacific white shrimp Litopenaeus vannamei, reared at different hatcheries in the Brazilian coast, was assessed by RAPD analysis. Fifty two polymorphic loci were identified when a set of five decamer primers was used in PCR. The genetic diversity analysis within lines evidenced genetic variation loss probably related to bottleneck effects and inbreeding. In addition, the genetic divergence values between the different samples appear to reflect the initial founder composition of such stocks, in some cases, sharing a common origin, suggesting a putative importance of interbreeding for the establishment of genetic improvement programs for these broodstocks. The genetic variation monitoring appears to be helpful to the gene pool conservation of this aquaculture species, mainly if considered its exotic status in Brazil and the current impossibility of new introduction of wild individuals.
authors.affiliation: Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil. patdf@iris.ufscar.br
authors.forename: P D
authors.initials: PD
authors.lastname: Freitas
authors.forename: M R
authors.initials: MR
authors.lastname: Calgaro
authors.forename: P M
authors.initials: PM
authors.lastname: Galetti
mesh_terms: D000818:Animals; D017756:Aquaculture; D001947:Breeding / Q000379:methods; D014644:Genetic Variation; D033561:Penaeidae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique