title: Expression of cell surface receptors and oxidative metabolism modulation in the clinical continuum of sepsis.
abstract: BACKGROUND
  Infection control depends on adequate microbe recognition and cell activation, yet inflammatory response may lead to organ dysfunction in sepsis. The aims of this study were to evaluate cell activation in the context of sepsis and its correlation with organ dysfunction.
  
  
  METHODS
  A total of 41 patients were prospectively enrolled: 14 with sepsis, 12 with severe sepsis and 15 with septic shock. A total of 17 healthy volunteers were included as a control group. Patients were admitted to the Intensive Care Units and Emergency Rooms of Hospital Sao Paulo (Federal University of Sao Paulo) and Hospital Santa Marcelina, Sao Paulo, Brazil. Toll-like receptor (TLR)2, TLR4, CD11b, CD11c and CD66b expression on neutrophil surfaces and oxidative metabolism measured by non-fluorescent dichlorofluorescein (DCFH) oxidation in neutrophils and monocytes, using whole blood, were evaluated using flow cytometry. Organ dysfunction was measured using the sepsis-associated organ failure assessment (SOFA) score.
  
  
  RESULTS
  TLR2 expression on neutrophils was found to be downregulated in septic shock patients compared to healthy volunteers (p = 0.05). No differences were found in CD11b and CD11c expression. CD66b expression was increased in the patient group compared to the control group (p = 0.01). Neutrophil and monocyte oxidative burst was increased in septic patients compared to the control group at baseline and after stimulation with phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (fMLP), lipopolysaccharide (LPS) and Staphylococcus aureus (p < 0.001 and p < 0.01, respectively, for neutrophils and monocytes in all tested conditions). A strong correlation was observed between neutrophil and monocyte oxidative metabolism. A SOFA score of 7 discriminated patients between survivors and non-survivors (area under the curve for reactive oxygen species (ROS) was 0.78; p = 0.02). ROS generation in patients with sepsis and septic shock with SOFA scores > 7 was higher than in patients with SOFA scores < 7, both in neutrophils and monocytes. However, oxidative burst in patients with sepsis was as high as in septic shock.
  
  
  CONCLUSIONS
  Surface receptors expression on neutrophils may be modulated across the continuum of sepsis, and enhanced or decreased expression may be found depending on the receptor considered. ROS generation is upregulated both in neutrophils and monocytes in septic patients, and it is differently modulated depending on the stage of the disease and the stimuli used.
authors.affiliation: Division of Infectious Diseases, Escola Paulista de Medicina, Federal University of Sao Paulo, Brazil.
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Martins
authors.forename: Milena K C
authors.initials: MK
authors.lastname: Brunialti
authors.forename: Leandro S W
authors.initials: LS
authors.lastname: Martos
authors.forename: Flavia R
authors.initials: FR
authors.lastname: Machado
authors.forename: Murillo S
authors.initials: MS
authors.lastname: Assunçao
authors.forename: Sergio
authors.initials: S
authors.lastname: Blecher
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Salomao
mesh_terms: D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009102:Multiple Organ Failure / Q000209:etiology / Q000503:physiopathology; D009504:Neutrophils / Q000378:metabolism*; D011446:Prospective Studies; D012372:ROC Curve; D017382:Reactive Oxygen Species / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018805:Sepsis / Q000145:classification / Q000378:metabolism* / Q000382:microbiology; D012720:Severity of Illness Index; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D011956:Receptors, Cell Surface; D051193:Toll-Like Receptors