[Removal of pentachlorophenol catalyzed by immobilized horseradish peroxidase].
The removal of PCP using HRP immobilized by Fe3O4 sorption-gelatin embedding-cross linkage method as catalyzer was studied. Reaction conditions including the reacting time, different buffer systems and pH value, PCP initial concentration, HRP dosage were discussed in detail compared with free HRP. The results indicate that the equilibrium time of PCP removal reaction catalyzed by immobilized HRP is about 30 min, which is as fast as free HRP. The optimal pH for PCP removal by immobilized HRP is between 4-6, the proper pH should be more extensive than using free HRP. The highest catalyzing removal percent is 41% at pH 5. Low concentration of immobilized HRP can remove PCP effectively. PCP removal quantity increases but the removal percent decreases with the increase of initial concentration of PCP. The catalyzing removal percent goes down from 39.68% to 24.4%. Immobilized HRP can remove PCP repetitively. The catalyzing removal percent is still above 39% at 0.05 U/mL dosage of HRP when using 7 times repeatedly.
Zong
Yue-ru
YR
Department of Environmental Science, College of Environmental Science, Peking University, Beijing 100871, China. zong@pku.edu.cn
Zhang
Jian-bo
JB
Wang
Shan-lin
SL
Yang
Yu-xiang
YX
Wang
Wei-jing
WJ
D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010416:Pentachlorophenol / Q000737:chemistry* / Q000302:isolation & purification; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods*
D004800:Enzymes, Immobilized; D014874:Water Pollutants, Chemical; D010416:Pentachlorophenol; D006735:Horseradish Peroxidase