MMP-14 mediated MMP-9 expression is involved in TGF-beta1-induced keratinocyte migration.
The importance of expression of matrix metalloproteinase (MMP) in keratinocyte migration is well established, but its role remains unclear. Here we investigated the function of MMP-14 in TGF-beta1-induced keratinocyte migration. TGF-beta1 stimulated cell migration and the expression of MMP-2, -9 in HaCaT human keratinocyte cells. When we lowered MMP-14 mRNA with siRNA, cell migration, and MMP-9 expression decreased. Furthermore, the MMP-14 siRNA also reduced activation of JNK in response to TGF-beta1, and a JNK-specific inhibitor decreased both cell migration and MMP-9 expression. Taken together, these results suggest that TGF-beta1-induced HaCaT cell migration is mediated by MMP-14, which regulates MMP-9 expression via JNK signaling.
Seomun
Young
Y
Laboratory of Ophthalmology and Visual Science, Korean Eye Tissue and Gene Bank Related to Blindness, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Kim
Jong-Tak
JT
Joo
Choun-Ki
CK
D002450:Cell Communication; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D020935:MAP Kinase Signaling System; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008954:Models, Biological; D010766:Phosphorylation; D015398:Signal Transduction; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
D053773:Transforming Growth Factor beta1; D048670:MAP Kinase Kinase 4; D020780:Matrix Metalloproteinase 9; D053511:Matrix Metalloproteinase 14