title: Detection of enteroviral RNA on Guthrie card dried blood of a neonate with fatal Coxsackie B3 myocarditis on day 17.
abstract: A fatal case of Coxsackie B3 myocarditis in a neonate is described. The clinical features became evident in the 3rd week of life, but enteroviral RNA was detected in the dried blood spot of the baby collected on day 4 after birth. This is the first report on the detection of enteroviral RNA in Guthrie card dried blood using reverse-transcriptase PCR. Materials and methods used are described in detail.
authors.affiliation: Department of Neonatology, Ghent University Hospital, De Pintelaan 185, B-9000 Gent, Belgium. koenraad.smets@ugent.be
authors.forename: Koenraad
authors.initials: K
authors.lastname: Smets
authors.forename: Annelies
authors.initials: A
authors.lastname: Keymeulen
authors.forename: Elke
authors.initials: E
authors.lastname: Wollants
authors.forename: Katrien
authors.initials: K
authors.lastname: Lagrou
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
authors.forename: Elizaveta
authors.initials: E
authors.lastname: Padalko
mesh_terms: D001800:Blood Specimen Collection / Q000379:methods*; D003384:Coxsackievirus Infections / Q000175:diagnosis / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017809:Fatal Outcome; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009205:Myocarditis / Q000175:diagnosis / Q000821:virology*; D012367:RNA, Viral / Q000097:blood* / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral