Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants.
The substrate binding regions of a beta-1,3:1,4 glucanase are revealed through structural analysis with a thio-oligosaccharide and kinetics of enzyme variants.
Money
Victoria A
VA
Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington, York, U.K.YO10 5YW.
Cartmell
Alan
A
Guerreiro
Catarina I P D
CI
Ducros
Valerie M-A
VM
Fontes
Carlos M G A
CM
Gilbert
Harry J
HJ
Davies
Gideon J
GJ
D001665:Binding Sites; D002236:Carbohydrate Conformation; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry*; D007700:Kinetics; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D009844:Oligosaccharides; D013438:Sulfhydryl Compounds; D006026:Glycoside Hydrolases; C028020:licheninase