Formation of a cross-linking complex of dinitrogenase reductase-activating glycohydrolase (DRAG) with membrane proteins from Rhodospirillum rubrum chromatophores.
Association of dinitrogenase reductase-activating glycohydrolase (DRAG) with membrane proteins of chromatophores has been investigated. The formation of a multicomponent complex between DRAG and membrane proteins was demonstrated in the presence of glutaraldehyde and EDC/NHS (N-(3-dimethylaminopropyl)-N -ethylcarbodiimide hydrochloride/hydroxy-2,5-dioxopyrrolidine-3-sulfonic acid sodium salt). Complex formation was observed both in native chromatophore membrane and in chromatophores treated with 0.5 M NaCl in the presence of homogeneous DRAG and glutaraldehyde in cross-reaction. The molecular weight of the complex was around 200 kD, which is consistent with the association of DRAG with three or more chromatophore membrane proteins. A specific complex with molecular weight of about 75 kD was formed only in the presence of EDC/NHS in the cross-linking reaction. It was demonstrated that ammonium transport protein and P11 protein are possible candidates for association with DRAG in chromatophore membranes.
Akentieva
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Washington University in Saint Louis, Saint Louis, MO 63110, USA. akentevan@morpheus.wustl.edu
D020130:Bacterial Chromatophores / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003432:Cross-Linking Reagents; D005976:Glutaral / Q000737:chemistry; D008565:Membrane Proteins / Q000378:metabolism*; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D012247:Rhodospirillum rubrum / Q000378:metabolism*
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D009699:N-Glycosyl Hydrolases; C036091:ADP-ribosyl-(dinitrogen reductase) hydrolase; D005976:Glutaral