title: Identification, molecular structure and expression of two cloned serotonin receptors from the pond snail, Helisoma trivolvis.
abstract: Helisoma trivolvis has served as a model system to study the functions of serotonin (5-HT) from cellular, developmental, physiological and behavioural perspectives. To further explore the serotonin system at the molecular level, and to provide experimental knockout tools for future studies, in this study we identified serotonin receptor genes from the H. trivolvis genome, and characterized the molecular structure and expression profile of the serotonin receptor gene products. Degenerate oligonucleotide primers, based on conserved regions of the Lymnaea stagnalis 5-HT(1Lym) receptor, were used to amplify G protein-coupled biogenic amine receptor sequences from H. trivolvis genomic cDNA, resulting in the cloning of two putative serotonin receptors. The deduced gene products both appear to be G protein-coupled serotonin receptors, with well-conserved structure in the functional domains and high variability in the vestibule entrance of the receptor protein. Phylogenetic analysis placed these receptors in the 5-HT(1) and 5-HT(7) families of serotonin receptors. They are thus named the 5-HT(1Hel) and 5-HT(7Hel) receptors, respectively. In situ hybridization and immunofluorescence studies revealed that these genes and gene products are expressed most heavily in the ciliated pedal and mantle epithelia of H. trivolvis embryos. In adults, widespread expression occurred in all ganglia and connectives of the central nervous system. Expression of both receptor proteins was localized exclusively to neurites when examined in situ. In contrast, when isolated neurons were grown in culture, 5-HT(1Hel) and 5-HT(7Hel) immunoreactivity were located primarily in the cell body. This is the first study to reveal a 5-HT(7) receptor in a molluscan species.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada, T6G 2E9.
authors.forename: Sabeen
authors.initials: S
authors.lastname: Mapara
authors.forename: Shawn
authors.initials: S
authors.lastname: Parries
authors.forename: Caitlin
authors.initials: C
authors.lastname: Quarrington
authors.forename: Kee-Chan
authors.initials: KC
authors.lastname: Ahn
authors.forename: Warren J
authors.initials: WJ
authors.lastname: Gallin
authors.forename: Jeffrey I
authors.initials: JI
authors.lastname: Goldberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D011985:Receptors, Serotonin / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012908:Snails / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D017931:DNA Primers; D011985:Receptors, Serotonin