title: Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
abstract: Helicobacter pylori are bacteria that colonize the stomach persistently, which confers risk of serious diseases, including peptic ulceration and gastric neoplasia. Aberrant expression of cell cycle control proteins has been demonstrated in H. pylori infected gastric epithelial cells, suggesting that perturbation of the cell cycle plays a role in the pathogenesis of various H. pylori associated diseases. In this study, we investigate the modulation of the cell cycle control protein p21(WAF1) by H. pylori in the gastric carcinoma cell line NCI-N87 and in primary gastric cells derived from healthy tissue. We observed an up-regulation of p21(WAF1) in both NCI-N87 and primary cells. Chromatin immunoprecipitation analysis revealed that the increased expression of p21(WAF1) induced by H. pylori is associated with the release of HDAC-1 from the p21(WAF1) promoter and hyper-acetylation of histone H4. The elucidation of the epigenetic regulation of p21(WAF1) by H. pylori may help to dissect the pathogenetic mechanisms underlying the development and progression of H. pylori associated diseases.
authors.affiliation: Institute of Experimental Internal Medicine, University Hospital Magdeburg, Otto von Guericke University Magdeburg, Leipziger Street 44, D-39120 Magdeburg, Germany.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Xia
authors.forename: Regine
authors.initials: R
authors.lastname: Schneider-Stock
authors.forename: Antje
authors.initials: A
authors.lastname: Diestel
authors.forename: Caroline
authors.initials: C
authors.lastname: Habold
authors.forename: Sabine
authors.initials: S
authors.lastname: Krueger
authors.forename: Albert
authors.initials: A
authors.lastname: Roessner
authors.forename: Michael
authors.initials: M
authors.lastname: Naumann
authors.forename: Uwe
authors.initials: U
authors.lastname: Lendeckel
mesh_terms: D000107:Acetylation; D002478:Cells, Cultured; D005753:Gastric Mucosa / Q000378:metabolism* / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016480:Helicobacter pylori / Q000502:physiology*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D015689:Oncogene Protein p21(ras) / Q000378:metabolism*
chemical_list: D006657:Histones; D015689:Oncogene Protein p21(ras)