Molecular characterization and mRNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder (Paralichthys olivaceus).
Glutathione peroxidase (GPX) and glutathione S-transferase (GST) are key enzymes of cellular detoxification systems that defend cells against reactive oxygen species (ROS). In this study, we isolated the GPX and GST full-length cDNA and investigated the expression of these mRNAs from livers of olive flounder during salinity changes (35, 17.5, 8.75, 4 and 0 psu) by quantitative PCR (QPCR). GPX cDNA consists of 429 base pairs (bp) and encodes a protein of 142 amino acids. GST cDNA consists of 663 bp and encodes a protein of 220 amino acids. Both of GPX and GST mRNA expressions were the highest in 4 psu and then decreased in 0 psu. Also, the levels of Na(+) and Cl(-) decreased, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) increased during the experimental period. These findings provide molecular characterization of GPX and GST in olive flounder and suggest that GPX and GST play important roles in detoxification of ROS, thereby these maybe indicators of oxidative stress responses by salinity changes in olive flounder.
Choi
Cheol Young
CY
Division of Marine Environment and Bioscience, Korea Maritime University, Busan 606-791, Republic of Korea.
An
Kwang Wook
KW
An
Myung In
MI
D000222:Adaptation, Physiological / Q000235:genetics; D000410:Alanine Transaminase / Q000097:blood; D000595:Amino Acid Sequence; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002712:Chlorides / Q000097:blood; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D005432:Flounder / Q000097:blood / Q000235:genetics* / Q000502:physiology; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000097:blood; D013312:Stress, Physiological / Q000235:genetics*
D002712:Chlorides; D018076:DNA, Complementary; D012333:RNA, Messenger; D012964:Sodium; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase