Sequencing, characterization and genetic variation of the Bos indicus glucose-6-phosphate-dehydrogenase gene.
The coding sequence of the bovine (Bos indicus) Glucose-6-phosphate-dehydrogenase (G6PD) gene was amplified by Reverse Transcriptase-PCR (RT-PCR), cloned, sequenced and characterized. The deduced amino acid sequence clustered the bovine G6PD sequence with the other mammalian G6PD proteins into a monophyletic group. The bovids (B. indicus and B. taurus) clustered clearly from the rodent (rat, mouse and hamster) subcluster and from humans. The multiple sequence alignment of the bovine G6PD with the mammalian species clearly revealed conservation of the substrate, coenzyme, catalytic and the dimer binding sites with the solved X-ray crystallographic structure of Homo sapiens. Also, four fragments of bovine (Bos indicus) G6PD gene viz. 118, 319, 683 and 408 bp were amplified and sequenced for the first time. A G/A and G/C single nucleotide polymorphisms in intron-9 and exon-10 were detected on PCR-RFLP of the 319 bp amplicon with Hae III and Pst I, respectively. This work is the first study on Bos indicus G6PD gene at the nucleotide level.
Ravi Kumar
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GV
Molecular Cytogenetics Lab, Department of Animal Breeding and Genetics, GADVASU, PAU Campus, Ludhiana 141004, India. gandham71@gmail.com
Srinivas
G
G
Sharma
A
A
Suryanarayana
V V S
VV
Ravi Kumar
P
P
Bhattacharya
T K
TK
Mitra
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA
D012333:RNA, Messenger; D005954:Glucosephosphate Dehydrogenase