title: Isolation and potential existence of side population cells in adult human kidney.
abstract: The existence of adult stem-like cells such as side population (SP) cells is reported in various kinds of animal tissues, and we recently reported that mice kidney SP cells differentiate into multilineage. However, there has thus far been no report about human kidney SP cells. In the present study, we examined the existence of SP cells in human kidney tissue by using Hoechst 33342 staining and fluorescence-activated cell sorting analysis. We used porcine kidney tissue to optimize the analysis conditions for human tissue, and found that the SP population in human kidney was 1.3%. The existence of SP cells in human kidney suggests that the cells could be good targets for clinical renal regenerative medicine.
authors.affiliation: Department of Urology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Inowa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hishikawa
authors.forename: Takumi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Tadaichi
authors.initials: T
authors.lastname: Kitamura
authors.forename: Toshiro
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002469:Cell Separation; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D013234:Stem Cells; D013552:Swine