title: [Foxp3-transfected CD4+ CD25- T cells suppress function of dendritic cells].
abstract: To explore the relationship between expression of Foxp3 gene and immune activity of CD4(+) T cells, the Foxp3 gene was transfected with retroviral vector and applied to forcedly express Foxp3 protein in naive CD4(+)CD25(-) T cells, and then the effect of transfected CD4(+)CD25(-) T cells on immune co-stimulatory molecules and immune function of dendritic cells (DCs) was investigated, and the dependence of direct contact between Foxp3-transfected CD4(+)CD25(-) T cells and DCs was clarified by Transwell test. The results showed that through transfection of retroviral vector, CD4(+)CD25(-) T cells model expressing Foxp3 was established. At 1 week after transfection, proportion of T cells expressing Foxp3 was 38%. CD4(+)CD25(-) T cells forcedly expressing Foxp3 could play immune suppression role in vitro and induce down-regulation of CD80 and CD86 expression on the membrane of DCs. The lymphocyte proliferation test in vitro indicated that Foxp3 transfected CD4(+)CD25(-) T cells could inhibit effect of DCs on activation of allo-lymphocytes. It is concluded that the effect of Foxp3-transfected CD4(+)CD25(-) T cells on DC depends on intercellular direct contact.
authors.affiliation: Institute of Hematology, Union Hospital, Tongji Medical College, University of Science and Technology, Wuhan 430022, Hubei Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Zheng-Rong
authors.initials: ZR
authors.lastname: Liu
authors.forename: Ping
authors.initials: P
authors.lastname: Zou
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D007108:Immune Tolerance / Q000235:genetics*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012190:Retroviridae; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
chemical_list: D015704:CD4 Antigens; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit