Irradiated Cerastes cerastes venom as a novel tool for immunotherapy.
Immunotherapy is the most effective treatment for the snake bites. The antivenoms are commonly obtained by hyperimmunization of animals that suffer from venom toxicity. The present report describes gamma irradiation effects on Cerastes cerastes venom. Doses of 1 kGy and 2 kGy gamma radiations were used for venom detoxification. These treated venoms did not have any residual lethal effects until 10 LD(50). Immunological analysis of sera raised against native and irradiated venoms, showed that elicited antibodies to irradiated venoms were able to recognize native venom. Anti-2 kGy irradiated venom had more protective ability than anti-native venom, as tested in mice.
Oussedik-Oumehdi
Habiba
H
Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, USTHB Bab Ezzouar, Algiers, Algeria.
Laraba-Djebari
Fatima
F
D000818:Animals; D000906:Antibodies / Q000097:blood; D000997:Antivenins / Q000627:therapeutic use*; D005720:Gamma Rays; D007116:Immunization, Passive; D007167:Immunotherapy; D007928:Lethal Dose 50; D051379:Mice; D008815:Mice, Inbred Strains; D009500:Neutralization Tests; D011817:Rabbits; D012909:Snake Bites / Q000628:therapy*; D014757:Viper Venoms / Q000201:enzymology / Q000276:immunology* / Q000528:radiation effects*; D017819:Viperidae
D000906:Antibodies; D000997:Antivenins; D014757:Viper Venoms