An amperometric immunosensor for osteoproteogerin based on gold nanoparticles deposited conducting polymer.
An amperometric immunosensor was fabricated for the detection of osteoproteogerin (OPG) by covalently immobilizing a monoclonal OPG antibody (anti-OPG) onto the gold nanoparticles (AuNPs) deposited functionalized conducting polymer (5,2':5',2''-terthiophene-3'-carboxylic acid). AuNPs were electrochemically deposited onto the conducting polymer using cyclic voltammetry. The particle size of deposited AuNPs was controlled by varying the scan rate and was characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The immobilization of anti-OPG was also confirmed using XPS. The principle of immunosensor was based on a competitive immunoassay between free-OPG and labeled-OPG for the active sites of anti-OPG. HRP was used as a label that electrochemically catalyzes the H(2)O(2) reduction. The catalytic reduction was monitored amperometrically at -0.4V vs. Ag/AgCl. The immunosensor showed a linear range between 2.5 and 25pg/ml and the detection limit was determined to be 2pg/ml. The proposed immunosensor was successfully applied for real human samples to detect OPG.
Singh
Kanika
K
School of Mechanical Engineering and MEMS/Nano Technology Center, Pusan National University, Pusan 609-735, South Korea.
Rahman
Md Aminur
MA
Son
Jung Ik
JI
Kim
Kyung Chun
KC
Shim
Yoon-Bo
YB
D015374:Biosensing Techniques / Q000295:instrumentation*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D004595:Electroplating; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry*; D053244:Osteoprotegerin / Q000032:analysis* / Q000276:immunology; D011108:Polymers / Q000737:chemistry
D053244:Osteoprotegerin; D011108:Polymers; D006046:Gold