[Optimization of preparative separation and purification of water-soluble substance from Salvia miltiorrhiza by macroporous resins].
OBJECTIVE
To study the optimum conditions for the isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge.


METHODS
The optimum macroporous resin was selected and the separation and purification process was evaluated by measuring the content of Salvianolic acid B in the fractions by HPLC.


RESULTS
The XDA-5 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 18 mg/ml corresponding to Salvianolic acid B for concentration of extract, pH was 4, and the volume of 70% (V/V) ethanol as eluant was 3 BV. By this method, the elution efficiency of Salvianolic acid B exceeded 90%.


CONCLUSIONS
The method is more effective for large-scale isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge.
He
Wei
W
Institute of Materia Medica, Guangdong College of Pharmacy, Guangzhou 510006, China. weih8201@163.com
Li
Yong
Y
Shen
Xue-mei
XM
Sun
Li-min
LM
D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D014867:Water; D053630:Depsides
D001572:Benzofurans; D000431:Ethanol; D012117:Resins, Synthetic; D014867:Water; C076944:salvianolic acid B; D053630:Depsides