Structure-and-mechanism-based design and discovery of therapeutics for cocaine overdose and addiction.
(-)-Cocaine is a widely abused drug and there is currently no available anti-cocaine therapeutic. Promising agents, such as anti-cocaine catalytic antibodies and high-activity mutants of human butyrylcholinesterase (BChE), for therapeutic treatment of cocaine overdose have been developed through structure-and-mechanism-based design and discovery. In particular, a unique computational design strategy based on the modeling and simulation of the rate-determining transition state has been developed and used to design and discover desirable high-activity mutants of BChE. One of the discovered high-activity mutants of BChE has a approximately 456-fold improved catalytic efficiency against (-)-cocaine. The encouraging outcome of the structure-and-mechanism-based design and discovery effort demonstrates that the unique computational design approach based on transition state modeling and simulation is promising for rational enzyme redesign and drug discovery. The general approach of the structure-and-mechanism-based design and discovery may be used to design high-activity mutants of any enzyme or catalytic antibody.
Zheng
Fang
F
Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 725 Rose Street, Lexington, KY 40536, USA.
Zhan
Chang-Guo
CG
D000818:Animals; D017151:Antibodies, Catalytic / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D002091:Butyrylcholinesterase / Q000737:chemistry* / Q000378:metabolism / Q000627:therapeutic use; D002384:Catalysis / Q000187:drug effects; D003042:Cocaine / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000506:poisoning; D019970:Cocaine-Related Disorders / Q000188:drug therapy*; D015195:Drug Design; D062787:Drug Overdose; D006801:Humans; D006868:Hydrolysis / Q000187:drug effects; D008968:Molecular Conformation; D013329:Structure-Activity Relationship
D017151:Antibodies, Catalytic; D002091:Butyrylcholinesterase; D003042:Cocaine