title: Identification of an antiparallel coiled-coil/loop domain required for ligand binding by the Borrelia hermsii FhbA protein: additional evidence for the role of FhbA in the host-pathogen interaction.
abstract: Borrelia hermsii, an etiological agent of tick-borne relapsing fever in North America, binds host-derived serum proteins including factor H (FH), plasminogen, and an unidentified 60-kDa protein via its FhbA protein. Two distinct phylogenetic types of FhbA have been delineated (FhbA1 and FhbA2). These orthologs share a conserved C-terminal domain that contains two alpha helices with a high predictive probability of coiled-coil formation that are separated by a 14-amino-acid loop domain. Through site-directed mutagenesis, we have identified residues within these domains that influence the binding of both mouse and human FH, plasminogen, and/or the 60-kDa protein. To further investigate the involvement of FhbA in the host-pathogen interaction, strains that are either FhbA(+) (isolate YOR) or FhbA(-) (isolate REN) were tested for serum sensitivity. Significant differences were observed, with YOR and REN being serum resistant and serum sensitive (intermediate), respectively. To test the abilities of these strains to infect and persist in mice, mice were needle inoculated, and infectivity and persistence were then assessed. While both strains REN and YOR infected mice, only the FhbA(+) YOR strain persisted beyond day 4. Survival of the YOR isolate in blood correlated with the upregulation of the fhbA gene, as demonstrated by real-time reverse transcriptase PCR. These data advance our understanding of the unique interactions of FhbA with individual serum proteins and provide support for the hypothesis that FhbA is an important contributor to the pathogenesis of the relapsing fever spirochete B. hermsii.
authors.affiliation: Department of Microbiology and Immunology, Medical College of Virginia at Virginia Commonwealth University, 1112 E. Clay St., Richmond, VA 23298-0678, USA.
authors.forename: Kelley M
authors.initials: KM
authors.lastname: Hovis
authors.forename: John C
authors.initials: JC
authors.lastname: Freedman
authors.forename: Hongming
authors.initials: H
authors.lastname: Zhang
authors.forename: Jonathan L
authors.initials: JL
authors.lastname: Forbes
authors.forename: Richard T
authors.initials: RT
authors.lastname: Marconi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001769:Blood / Q000382:microbiology; D001770:Blood Bactericidal Activity; D001898:Borrelia / Q000235:genetics / Q000378:metabolism*; D001899:Borrelia Infections / Q000382:microbiology; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017242:Complement Factor H / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D050296:Microbial Viability; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010958:Plasminogen / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013997:Time Factors
chemical_list: D002352:Carrier Proteins; C510009:FH-binding protein A, Borrelia hermsii; D017242:Complement Factor H; D010958:Plasminogen