Lipopolysaccharide binding protein and sCD14 are not produced as acute phase proteins in cardiac surgery.
OBJECTIVE
The changes in the serum levels of lipopolysaccharide binding protein (LBP) and sCD14 during cardiac surgery were followed in this study.


METHODS
Thirty-four patients, 17 in each group, were randomly assigned to coronary artery bypass grafting surgery performed either with ("on-pump") or without ("off-pump") cardiopulmonary bypass. LBP and sCD14 were evaluated by ELISA.


RESULTS
The serum levels of LBP were gradually increased from the 1st postoperative day and reached their maximum on the 3rd postoperative day in both "on-pump" and "off-pump" patients (30.33+/-9.96 microg/mL; 37.99+/-16.58 microg/mL), respectively. There were no significant differences between "on-pump" and "off-pump" patients regarding LBP. The significantly increased levels of sCD14 from the 1st up to the 7th postoperative day in both "on-pump" and "off-pump" patients were found with no significant differences between these groups. No correlations between LBP and sCD14 and IL-6, CRP and long pentraxin PTX3 levels were found.


CONCLUSIONS
The levels of LBP and sCD14 are elevated in cardiac surgical patients being similar in both groups. These molecules are not produced as acute phase proteins in these patients.
Kudlova
Manuela
M
Institute of Clinical Immunology and Allergology, Charles University in Prague, School of Medicine and University Hospital in Hradec Králové, 500 05 Hradec Kralove , Czech Republic.
Kunes
Pavel
P
Kolackova
Martina
M
Lonsky
Vladimir
V
Mandak
Jiri
J
Andrys
Ctirad
C
Jankovicova
Karolina
K
Krejsek
Jan
J
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D002352:Carrier Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000097:blood / Q000737:chemistry*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D008954:Models, Biological; D015233:Models, Statistical; D000683:Serum Amyloid P-Component / Q000378:metabolism; D013997:Time Factors; D000099231:Lipopolysaccharide-Binding Protein; D000099153:Pentraxins
D000209:Acute-Phase Proteins; D002097:C-Reactive Protein; D002352:Carrier Proteins; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D000683:Serum Amyloid P-Component; D000099231:Lipopolysaccharide-Binding Protein; D000099153:Pentraxins