Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme.
The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1-->3,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows: [Formula: see text].
van Leeuwen
Sander S
SS
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Kralj
Slavko
S
van Geel-Schutten
Ineke H
IH
Gerwig
Gerrit J
GJ
Dijkhuizen
Lubbert
L
Kamerling
Johannis P
JP
D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D052200:Limosilactobacillus reuteri / Q000737:chemistry / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005936:Glucans; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; C009288:metaperiodate; D016695:Glycosyltransferases; C033837:alternansucrase