Insight into initiator-DNA interactions: a lesson from the archaeal ORC.
Although initiation of DNA replication is considered to be highly coordinated through multiple protein-DNA and protein-protein interactions, it is poorly understood how particular locations within the eukaryotic chromosome are selected as origins of DNA replication. Here, we discuss recent reports that present structural information on the interaction characteristics of the archaeal orthologues of the eukaryotic origin recognition complex with their cognate binding sequences. Since the archaeal replication system is postulated as a simplified version of the one in eukaryotes, by analogy, these works provide insights into the functions of the eukaryotic initiator proteins.
Tada
Shusuke
S
Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan. tada@mail.pharm.tohoku.ac.jp
Kundu
Lena R
LR
Enomoto
Takemi
T
D019843:Archaeal Proteins / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002874:Chromosome Mapping; D002875:Chromosomes; D004247:DNA / Q000378:metabolism*; D004261:DNA Replication; D004926:Escherichia coli / Q000378:metabolism; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D051738:Origin Recognition Complex; D017434:Protein Structure, Tertiary; D018741:Replication Origin
D019843:Archaeal Proteins; D001426:Bacterial Proteins; D051738:Origin Recognition Complex; D004247:DNA