Editing and escape from editing in anti-DNA B cells.
Tolerance to dsDNA is achieved through editing of Ig receptors that react with dsDNA. Nevertheless, some B cells with anti-dsDNA receptors escape editing and migrate to the spleen. Certain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transgenic mice also bind an additional, Golgi-associated antigen. B cells that bind this antigen accumulate intracellular IgM. The intracellular accumulation of IgM is incomplete, because IgM clusters are observed at the cell surface. In the spleen, B cells that express the heavy and light chains encoding this IgM are surface IgM-bright and acquire the CD21-high/CD23-low phenotype of marginal zone B cells. Our data imply that expression of an Ig that binds dsDNA and an additional antigen expressed in the secretory compartment renders B cells resistant to central tolerance. In the periphery, these B cells may be sequestered in the splenic marginal zone.
Khan
Salar N
SN
Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Witsch
Esther J
EJ
Goodman
Noah G
NG
Panigrahi
Anil K
AK
Chen
Ching
C
Jiang
Yufei
Y
Cline
Amy M
AM
Erikson
Jan
J
Weigert
Martin
M
Prak
Eline T Luning
ET
Radic
Marko
M
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D015322:Gene Rearrangement, B-Lymphocyte; D006801:Humans; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000276:immunology; D019169:Jurkat Cells; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010718:Phosphatidylserines / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000276:immunology; D017634:Self Tolerance
D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D010718:Phosphatidylserines; D011947:Receptors, Antigen, B-Cell; D004247:DNA