Preparation of a Tat-related transporter peptide for carrying the adenovirus vector into cells.
We synthesized a Tat-related peptide acetyl-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly-Cys amide, Ac-Tat(48-60)-Gly-Cys-NH(2), having high intracellular permeability, and conjugated this peptide to adenovirus vector to enhance gene transfer efficiency of adenovirus vector into cells. The peptide was prepared by the solid-phase peptide synthesis method and a bifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide ester was used to conjugate the peptide to adenovirus vector containing luciferase gene. The novel conjugate of adenovirus vector and Ac-Tat(48-60)-Gly-Cys-NH(2) peptide exhibited excellent gene transfer efficacy in B16BL6 cells.
Kida
Shinya
S
Kobe Gakuin University, Minatojima, Chuo-ku, Kobe 650-8586, Japan.
Eto
Yusuke
Y
Maeda
Mitsuko
M
Yoshioka
Yasuo
Y
Nakagawa
Shinsaku
S
Hojo
Keiko
K
Tsuda
Yuko
Y
Mayumia
Tadanori
T
Kawasaki
Koichi
K
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D015696:Gene Products, tat; D010455:Peptides