title: Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlow.
abstract: We developed the simple, rapid (1 h), and accurate PNA FISH(Flow) method for the identification of Candida albicans. The method exploits unique in solution in situ hybridization conditions under which the cells are simultaneously fixed and hybridized. This method facilitates the accurate identification of clinical yeast isolates using two scoring techniques: flow cytometry and fluorescence microscopy.
authors.affiliation: AdvanDx, Inc., 10A Roessler Road, Woburn, MA 01801, USA. jtr@advandx.com
authors.forename: Jan
authors.initials: J
authors.lastname: Trnovsky
authors.forename: William
authors.initials: W
authors.lastname: Merz
authors.forename: Phyllis
authors.initials: P
authors.lastname: Della-Latta
authors.forename: Fann
authors.initials: F
authors.lastname: Wu
authors.forename: Maiken Cavling
authors.initials: MC
authors.lastname: Arendrup
authors.forename: Henrik
authors.initials: H
authors.lastname: Stender
mesh_terms: D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D015341:Nucleic Acid Probes / Q000235:genetics*; D020135:Peptide Nucleic Acids; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D011933:Reagent Kits, Diagnostic