Calix[4]arene decorated with four Tn antigen glycomimetic units and P3CS immunoadjuvant: synthesis, characterization, and anticancer immunological evaluation.
A novel anticancer vaccine candidate built on a nonpeptidic scaffold has been synthesized. Four S-Tn tumor-associated glycomimetic antigens have been clustered onto a calix[4]arene scaffold bearing an immunoadjuvant moiety (P3CS). The immunogenicity of the synthetic construct has been investigated by immunization of mice in vivo. ELISA assay has evidenced that the tetravalent construct stimulates a higher production of anti-Tn antigen IgG antibodies when compared to an analogous monovalent compound. This result is ascribable to an antigen cluster effect and makes the reported vaccine candidate a good mimic of the natural motifs present on the mucine surface.
Geraci
Corrada
C
Istituto di Chimica Biomolecolare, CNR, Via del Santuario 110, I-95028 Valverde (CT), Italy.
Consoli
Grazia M L
GM
Galante
Eva
E
Bousquet
Ennio
E
Pappalardo
Maria
M
Spadaro
Angelo
A
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000276:immunology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry* / Q000276:immunology*; D047250:Calixarenes / Q000737:chemistry* / Q000276:immunology*; D019496:Cancer Vaccines / Q000737:chemistry* / Q000276:immunology*; D002855:Chromatography, Thin Layer; D004151:Dipeptides / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007167:Immunotherapy / Q000379:methods*; D007202:Indicators and Reagents; D008074:Lipoproteins / Q000737:chemistry* / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008807:Mice, Inbred BALB C; D018716:Molecular Mimicry; D009369:Neoplasms / Q000276:immunology* / Q000517:prevention & control*; D010636:Phenols / Q000737:chemistry* / Q000276:immunology*; D021241:Spectrometry, Mass, Electrospray Ionization
D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D019496:Cancer Vaccines; D004151:Dipeptides; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D008074:Lipoproteins; D010636:Phenols; C040286:Tn antigen; C121325:calix(4)arene; D047250:Calixarenes; C047210:2,3-bis-(palmitoyloxy)-2-propyl-N-palmitoyl-cysteinylserine