title: Methodologies for staining and visualisation of beta-galactosidase in mouse embryos and tissues.
abstract: This chapter provides information on beta-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of beta-galactosidase can be visualized throughout development and in adult tissues. beta-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.
authors.affiliation: School of Biomedical Sciences, University of Nottingham, UK.
authors.forename: Siobhan
authors.initials: S
authors.lastname: Loughna
authors.forename: Deborah
authors.initials: D
authors.lastname: Henderson
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D004622:Embryo, Mammalian / Q000737:chemistry*; D017930:Genes, Reporter; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D013194:Staining and Labeling / Q000379:methods*; D001616:beta-Galactosidase / Q000032:analysis* / Q000235:genetics
chemical_list: D004396:Coloring Agents; D001616:beta-Galactosidase