title: Crem activator isoforms in normal and impaired human spermatogenesis analyzed by real time RT-PCR.
abstract: cAMP responsive element modulator (CREM) activator isoforms are involved in mammalian spermatogenesis and spermiogenesis. CREM proteins are highly expressed in postmeiotic germ cells of rodents and primates. Homozygous CREM inactivated mice exhibit round spermatid maturation arrest. The lack of CREM expression at both the mRNA and protein levels is associated with spermatid maturation arrest in infertile patients. Using real-time RT-PCR, we have examined the levels of CREM activator isoform mRNAs: CREMtheta1, CREMtheta2 and CREMt2 + Ex-gamma in gametogenic and interstitial cell fractions from normal human testis, in homogenized tissue samples from spermatogenic arrest and from testicular tumors. We have shown for the first time the presence of CREM activator isoform containing exon gamma (CREMtau2 + Exgamma) in normal human spermatogenesis. Among the three CREM isoforms, CREMtheta1 was expressed in its highest level in the male gonads. In comparison, CREMtheta2 mRNA was significantly less suggesting that the P3 promoter is much more active in human testis than the P4 promoter. Minimal-nill levels of mRNA for either of the CREM activator isoforms were detected in lymphocytes or in gonadal tissues from patients with SCOS (Sertoli Cell Only Syndrome). This data underlines the significance of CREMtheta1 isoform in the regulation of transcription during post-meiotic germ cell differentiation.
authors.affiliation: Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Fiszer
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Białas
authors.forename: Natalia
authors.initials: N
authors.lastname: Rozwadowska
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Kosicki
authors.forename: Piotr
authors.initials: P
authors.lastname: Jedrzejczak
authors.forename: Maciej
authors.initials: M
authors.lastname: Kurpisz
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D060588:LIM Domain Proteins; D008297:Male; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; C504656:FHL5 protein, human; C504657:Fhl5 protein, mouse; D060588:LIM Domain Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D014157:Transcription Factors; C117930:activator of CREM in testis (ACT)