Acetaldehyde differentially affects the growth of uterine leiomyomata and myometrial cells in tissue cultures.
OBJECTIVE
To examine the effect of alcohol and its derivatives on leiomyomata versus normal myometrium in cell culture.


METHODS
A study on human tissue cultures.


METHODS
A tertiary-care university hospital.


METHODS
Premenopausal women with uterine myomas.


METHODS
Obtaining paired cultures of normal myometrium and myomas from women undergoing hysterectomy.


METHODS
Analysis of the effect of ethanol and acetaldehyde on cell growth and the expression of relevant proteins.


RESULTS
Acetaldehyde statistically significantly inhibited the growth of myoma cells compared with normal myometrium. The level of alcohol dehydrogenase 1 (ADH1) protein was lower in myoma than in myometrial cells. The acetaldehyde dehydrogenase 1 (ALDH1) protein level was higher in myoma cells. Treatment with acetaldehyde resulted in a relative reduction of ALDH1 level in the myoma cells.


CONCLUSIONS
Acetaldehyde has an inhibitory effect on cell growth of myoma compared with normal myometrium. The reduced level of ADH1 and the increased level of ALDH1 proteins observed in myoma cell culture reduces the acetaldehyde level and thus may be involved in myoma cell growth.
Shveiky
David
D
Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, Ein-Kerem, Jerusalem, Israel. dshveiky@gmail.com
Shushan
Asher
A
Ben Bassat
Hannah
H
Klein
Benjamin Y
BY
Ben Meir
Assaf
A
Levitzky
Rubina
R
Rojansky
Nathan
N
D000079:Acetaldehyde / Q000378:metabolism / Q000633:toxicity*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007889:Leiomyoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D009215:Myometrium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D050697:Retinal Dehydrogenase; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*
D007527:Isoenzymes; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase; D000079:Acetaldehyde