title: Decreased susceptibility to antifungals in respiratory-deficient Kluyveromyces lactis mutants.
abstract: Decreased susceptibility of K. lactis mutants impaired in the function of cytochrome c, cytochrome c1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used.
authors.affiliation: Department of Microbiology and Virology, Comenius University in Bratislava, 842 15 Bratislava, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Sarinová
authors.forename: V
authors.initials: V
authors.lastname: Straková
authors.forename: K
authors.initials: K
authors.lastname: Balková
authors.forename: Y
authors.initials: Y
authors.lastname: Gbelská
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D015152:Blotting, Northern; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects; D025141:Drug Resistance, Fungal; D004579:Electron Transport / Q000187:drug effects* / Q000235:genetics; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D007716:Kluyveromyces / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D009097:Multienzyme Complexes / Q000378:metabolism; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism; D013261:Sterols / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D005656:Fungal Proteins; D009097:Multienzyme Complexes; D013261:Sterols; C025310:lyticase; D000666:Amphotericin B; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D010447:Peptide Hydrolases