title: Differential expression of system L amino acid transporters during wound healing process in the skin of young and old rats.
abstract: In order to elucidate the role of the system L-type amino acid transporters (LATs) in the wound healing process of aged and young subjects, we investigated the expression of LAT1, LAT2 and their subunit 4F2hc in the skin healing process after artificial wounds of dorsal skin in the young and old rats.
  
  
  METHODS
  The 1 cm full-thickness incisional wounds were made through the skin and panniculus carnosus muscle. The wounds were harvested at days 1, 3, 5 and 7 post-wounding, the experimental controls were harvested the skin of rat without wounds and the various analyses were performed.
  
  
  RESULTS
  In young rats, gradually and noticeable wound healing was detected, however, in old rats, wound healing was found to be greatly delayed. In young rats, the expression of LAT1 was increased rapidly on the day 1 after wound induction, on the other hand, in old rats, the expression of LAT1 after wound induction was not different from the control group. In young rats, the expression of LAT2 after the induction of wound was not different from the control group, however in old rats, the expression of LAT2 on the day 1 of wound induction was rapidly elevated.
  
  
  CONCLUSIONS
  These results suggest that the LAT1 and LAT2 increase in the wound healing process after cell injury in young and old rats, respectively.
authors.affiliation: Oral Biology Research Institute and The Second Stage of BK21, Chosun University College of Dentistry, 375 Seosukdong, Dong-gu, Gwangju 501-759, Korea.
authors.forename: Moon-Jin
authors.initials: MJ
authors.lastname: Jeong
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
authors.forename: Heung-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yeong Mu
authors.initials: YM
authors.lastname: Ko
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Park
authors.forename: Soon-Jeong
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Endou
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Kanai
authors.forename: Do-Seon
authors.initials: DS
authors.lastname: Lim
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
mesh_terms: D000375:Aging / Q000378:metabolism*; D027182:Amino Acid Transport System y+ / Q000096:biosynthesis*; D000818:Animals; D004195:Disease Models, Animal; D027301:Fusion Regulatory Protein 1, Light Chains / Q000096:biosynthesis*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000096:biosynthesis*; D008297:Male; D021122:Protein Subunits; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing
chemical_list: D027182:Amino Acid Transport System y+; D027301:Fusion Regulatory Protein 1, Light Chains; D027283:Large Neutral Amino Acid-Transporter 1; D021122:Protein Subunits; C486582:Slc7a8 protein, rat