title: Comparison of gene expression of mitogenic kinin path in adherent and non-adherent CD 34-stem cells using oligonucleotide microarrays.
abstract: One of the more interesting cells present in the umbilical cord blood - as far as their potential clinical use is concerned - are stem cells not presenting the CD34 antigen. These are the pluripotential cells with their biological properties similar to mesenchymal stem cells, with the ability to differentiate into such tissue types as bone, cartilage, nervous (to some extent), glia and muscle. The authors compared the activity of genes coding the proteins in mitogenic signal paths activated by kinin receptors using oligonucleotide microarrays in adherent and non-adherent CD 34- cells derived from umbilical cord blood. In the linear regression model with a 95% prognosis area for differentiating genes outside this area, the following genes were selected: c-jun (present in 3 isoforms) and c-fos. The fos and jun genes create the AP-1 transcriptive factor which regulates the expression of genes taking part in numerous cellular processes, including the cell cycle and mitosis. The obtained results shed some light on the molecular processes behind the MSC proliferation and are a starting point for further studies on the mesenchymal stem cell biology.
authors.affiliation: Gynecology and Obstetrics Department, Medical University of Silesia, Katowice, Poland. rstojko@slam.katowice.pl
authors.forename: Rafał
authors.initials: R
authors.lastname: Stojko
authors.forename: Andrzej
authors.initials: A
authors.lastname: Witek
authors.forename: Joanna
authors.initials: J
authors.lastname: Głogowska
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Chromy
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Wilk
authors.forename: Lukasz
authors.initials: L
authors.lastname: Witek
authors.forename: Monika
authors.initials: M
authors.lastname: Bojdys-Szyndlar
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Machaj
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Pojda
mesh_terms: D018952:Antigens, CD34 / Q000235:genetics*; D002448:Cell Adhesion; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007705:Kinins / Q000235:genetics*; D008934:Mitogens / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012044:Regression Analysis; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D007705:Kinins; D008934:Mitogens; D012333:RNA, Messenger