Selective adsorbent for the removal of immunoglobulin E in bronchial asthma.
Agar-gel resin with tryptophan as ligand was synthesized as an adsorbent for the removal of Immunoglobulin E (IgE) in bronchial asthma. Factors affecting the adsorption properties of the adsorbent, such as the different kind of amino acid linked to the carrier, NaOH concentration and amount of epichlorohydrin used in activation, were studied in detail. The effect of spacer on the adsorption capacity was also investigated. Results showed that the agar-tryptophan adsorbent with a spacer could enhance the adsorption capacity to 70.73% (1309.403IU/g agar-gel), which was higher than that without spacers (53%). The selectivity and hemocompatibility of the adsorbent were also examined, which showed good selectivity for IgE and satisfactory blood compatibility.
Yu
Yaoting
Y
The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, PR China.
Zhang
Hongmei
H
Wang
Weichao
W
Wang
Shenqi
S
D000327:Adsorption; D000362:Agar / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry; D001249:Asthma / Q000276:immunology*; D002021:Buffers; D003365:Costs and Cost Analysis; D005782:Gels / Q000737:chemistry; D006648:Histocompatibility; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D008024:Ligands; D010796:Phthalic Anhydrides / Q000737:chemistry; D012680:Sensitivity and Specificity; D012972:Sodium Hydroxide / Q000737:chemistry; D013387:Succinic Anhydrides / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
D000596:Amino Acids; D002021:Buffers; D005782:Gels; D008024:Ligands; D010796:Phthalic Anhydrides; D013387:Succinic Anhydrides; D007073:Immunoglobulin E; D012972:Sodium Hydroxide; C031801:succinic anhydride; D014364:Tryptophan; D000362:Agar; C043103:phthalic anhydride