Heme oxygenase-1 inducing constituent of Prunella vulgaris in HepG2 cells.
Inducible heme oxygenase (HO)-1 is known to play a major role in the pathogenesis of several diseases, and it protects cells against oxidant-mediated injury. The bioassay-guided fractionation of the EtOH extract of the flowered fruit-spike of Prunella vulgaris L. (Labiatae) yielded two ursane-type triterpenes, 3beta,23-dihydroxyurs-12-en-28-oic acid (23-hydroxyursolic acid) (1) and 3beta-hydroxyurs-12-en-28-oic acid (ursolic acid) (2). Western blotting demonstrated that treatment with compound 1 increased the expression of HO-1 in a dose-dependent manner in human liver-derived HepG2 cells. Investigation of structure-related HO-1 inducing activity suggested that the hydroxyl group at the C-23 position in the ursane skeleton is important for this activity.
Jeong
Gil-Saeng
GS
X-Ray Microscopy Research Center, Wonkwang University, Iksan 570-749, Korea.
An
Ren-Bo
RB
Pae
Hyun-Ock
HO
Oh
Gi-Su
GS
Chung
Hun-Taeg
HT
Kim
Youn-Chul
YC
D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004790:Enzyme Induction; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D051547:Heme Oxygenase-1 / Q000096:biosynthesis*; D022781:Hepatocytes / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D015394:Molecular Structure; D010936:Plant Extracts / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology*; D011208:Powders; D031347:Prunella / Q000737:chemistry*; D013329:Structure-Activity Relationship
D010936:Plant Extracts; D011208:Powders; D051547:Heme Oxygenase-1