title: Nuclear receptor location analyses in mammalian genomes: from gene regulation to regulatory networks.
abstract: Rapid progress in mapping nuclear receptor binding sites, referred to as "location analysis," has recently been achieved through the use of chromatin immunoprecipitation approaches. Location analysis can be performed on a single locus or cover a complete genome, and the resulting datasets can be probed to identify direct target genes and/or investigate the molecular mechanisms by which nuclear receptors control gene expression. In addition, when coupled with other genetic and functional genomics investigative methods, location analysis has proven to be a powerful tool with which to identify novel biological functions of nuclear receptors and build transcriptional regulatory networks. Thus, the knowledge gained from several recent chromatin immunoprecipitation-based studies has challenged basic concepts of nuclear receptor action, offered new insights into gene-regulatory mechanisms, and led to the identification of nuclear receptor-controlled biological functions.
authors.affiliation: McGill Cancer Centre, McGill University, Montréal, Québec, Canada.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Deblois
authors.forename: Vincent
authors.initials: V
authors.lastname: Giguère
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D016678:Genome; D023281:Genomics; D006801:Humans; D008954:Models, Biological; D011401:Promoter Regions, Genetic; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D004247:DNA