A non-variable L1-peptide displays high sensitivity and specificity for detecting women having human papillomavirus-associated cervical lesions.
Anti-human papillomavirus (HPV) antibody detection is promising technique for detecting women at risk of suffering cervical cancer, since potentially oncogenic, persistent, long-term HPV-infections elicit an antibody response which is rarely detected in transitory HPV-infection patients. We have identified a non-variable C-terminus L1-peptide, belonging to an alpha-helix surface exposed on L1-protein, specifically recognized by antibodies from HPV-associated cervical lesion patients. This peptide tested against 313 sera presented higher reactivity with antibodies from cervical cancer (OD mean 0.43+/-0.13) or cervical lesion patients (OD mean 0.41+/-0.17) than antibodies from normal cytology patients (OD mean 0.17+/-0.03). High-risk HPV-infected patients presented higher antibody reactivity (OD mean 0.36+/-0.17) than high-risk HPV-non-infected patients (OD mean 0.22+/-0.11). This peptide showed 88.36% sensitivity, 99.39% specificity and 94.21% correct classification of high risk-HPV cervical lesion or cervical cancer patients. This peptide should be taken into account for designing serological screening or diagnostic tests for use in a clinical scenario.
Urquiza
Mauricio
M
Fundation Instituto de Inmunología de Colombia, Cra 50 # 26-00, Bogota, Colombia. mauricio_urquiza@fidic.org.co
Guevara
Tatiana
T
Sanchez
Ricardo
R
Vanegas
Magnolia
M
Patarroyo
Manuel E
ME
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000737:chemistry / Q000276:immunology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000276:immunology*; D012372:ROC Curve; D012097:Repressor Proteins; D012307:Risk Factors; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D002583:Uterine Cervical Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology* / Q000821:virology
C068651:E6 protein, Human papillomavirus type 16; D039841:Leukocyte L1 Antigen Complex; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins