title: Noninvasive ultrasound elasticity imaging (UEI) of Crohn's disease: animal model.
abstract: Inflammation occurs in episodic flares in Crohn's disease, which are part of the waxing and waning course of the disease. Healing between flares allows the intestine to reconstitute its epithelium, but this healing results in the deposition of fibrotic scar tissue as part of the healing process. Repeated cycles of flares and healing often lead to clinically significant fibrosis and stenosis of the intestine. Patients are treated empirically with steroids, with their many side effects, in the hope that they will respond. Many patients would be better treated with surgery if we could identify which patients truly have intestinal fibrosis. Ultrasound elasticity imaging (UEI) offers the potential to radically improve the diagnosis and management of local tissue elastic property, particularly intestinal fibrosis. This method allows complete characterization of local intestine tissue with high spatial resolution. The feasibility of UEI on Crohn's disease is demonstrated by directly applying this technique to an animal model of inflammatory bowel disease (IBD). Five female Lewis rats (150-180g) were prepared with phosphate buffered solution (PBS) as a control group and six were prepared with repeated intrarectal administration of trinitrobenzenesulfonic acid (TNBS) as a disease group. Preliminary strain measurements differentiate the diseased colons from the normal colons (p < 0.0002) and compared well with direct mechanical measurements and histology (p < 0.0005). UEI provides a simple and accurate assessment of local severity of fibrosis. The preliminary results on an animal model also suggest the feasibility of translating this imaging technique directly to human subjects for both diagnosis and monitoring.
authors.affiliation: Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. kangkim@umich.edu
authors.forename: Kang
authors.initials: K
authors.lastname: Kim
authors.forename: Laura A
authors.initials: LA
authors.lastname: Johnson
authors.forename: Congxian
authors.initials: C
authors.lastname: Jia
authors.forename: Joel C
authors.initials: JC
authors.lastname: Joyce
authors.forename: Sujal
authors.initials: S
authors.lastname: Rangwalla
authors.forename: Peter D R
authors.initials: PD
authors.lastname: Higgins
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Rubin
mesh_terms: D000818:Animals; D003106:Colon / Q000000981:diagnostic imaging* / Q000473:pathology; D003424:Crohn Disease / Q000000981:diagnostic imaging* / Q000473:pathology; D004195:Disease Models, Animal; D004548:Elasticity; D054459:Elasticity Imaging Techniques / Q000379:methods*; D005260:Female; D005355:Fibrosis; D007090:Image Interpretation, Computer-Assisted; D066298:In Vitro Techniques; D051381:Rats; D011917:Rats, Inbred Lew; D012680:Sensitivity and Specificity; D013314:Stress, Mechanical; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D014302:Trinitrobenzenesulfonic Acid