title: [New fluorescent staining method to distinguish normal and malignant rectum tissues using terbium (III)-ciprofloxacin complex].
abstract: A new fluorescent staining method to distinguish normal and malignant rectum tissues using terbium(III)-Ciprofloxacin complex was investigated. The terbium (III) complex exhibits strong fluorescence and the staining process with the complex is very fast. It needs 3-5 minutes. The results showed that the fluorescence microscope images were similar to the images stained using traditional hematoxylin and eosin (H&E) methodology. The fluorescence spectra and FTIR spectra of normal and malignant rectum tissues were collected and the detection criterions for the malignant rectum tissues were the same as the staining results. In summary, the fluorescence staining may be developed as a simple and convenient method.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Joint Laboratory of Polymer Science and Materials, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Meng
authors.forename: Duan-fu
authors.initials: DF
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Du-jin
authors.initials: DJ
authors.lastname: Wang
authors.forename: Yi-zhuang
authors.initials: YZ
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Qi
authors.forename: Jin-guang
authors.initials: JG
authors.lastname: Wu
mesh_terms: D002939:Ciprofloxacin / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012004:Rectal Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D012007:Rectum / Q000737:chemistry / Q000166:cytology / Q000473:pathology*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D013725:Terbium / Q000737:chemistry*
chemical_list: D013725:Terbium; D002939:Ciprofloxacin