Production of 1,5-dihydroxy-3-methoxy-7-methylanthracene-9,10-dione by submerged culture of Shiraia bambusicola.
1,5-Dihydroxy-3-methoxy-7-methylanthracene-9,10- dione (shiraiarin) is a kind of antitumor and antibacterial anthraquinone, and was produced for the first time from the submerged fermentation of Shiraia bambusicola, as confirmed by ESI-MS and NMR. The production of shiraiarin was significantly influenced when varying the carbon source, and a high amount of shiraiarin was only achieved when using lactose. The production of shiraiarin was also stimulated when using NaNO3 as the nitrogen source, whereas other nitrogen sources inhibited its production. Shiraiarin was formed during the stationary phase with a pH value higher than 8. The production of shiraiarin was inhibited by sporulation.
Cai
Yujie
Y
Key Laboratory of Industrial Biotechnology, Southern Yangtze University, Huihe Road 170, WuXi, JiangSu, China. yu_jie@yahoo.com.cn
Ding
Yanrui
Y
Tao
Guanjun
G
Liao
Xiangru
X
D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001203:Ascomycota / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D018533:Biomass; D017554:Carbon Compounds, Inorganic / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D017672:Nitrogen Compounds / Q000378:metabolism
D000880:Anthraquinones; D017554:Carbon Compounds, Inorganic; D003470:Culture Media; D017672:Nitrogen Compounds