title: Proteomic analysis of S-nitrosylated proteins in Arabidopsis thaliana undergoing hypersensitive response.
abstract: Nitric oxide (NO) has a fundamental role in the plant hypersensitive disease resistance response (HR), and S-nitrosylation is emerging as an important mechanism for the transduction of its bioactivity. A key step toward elucidating the mechanisms by which NO functions during the HR is the identification of the proteins that are subjected to this PTM. By using a proteomic approach involving 2-DE and MS we characterized, for the first time, changes in S-nitrosylated proteins in Arabidopsis thaliana undergoing HR. The 16 S-nitrosylated proteins identified are mostly enzymes serving intermediary metabolism, signaling and antioxidant defense. The study of the effects of S-nitrosylation on the activity of the identified proteins and its role during the execution of the disease resistance response will help to understand S-nitrosylation function and significance in plants.
authors.affiliation: Dipartimento Scientifico e Tecnologico, Università degli Studi di Verona, Verona, Italy.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Romero-Puertas
authors.forename: Natascia
authors.initials: N
authors.lastname: Campostrini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mattè
authors.forename: Pier Giorgio
authors.initials: PG
authors.lastname: Righetti
authors.forename: Michele
authors.initials: M
authors.lastname: Perazzolli
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
authors.forename: Peter
authors.initials: P
authors.lastname: Roepstorff
authors.forename: Massimo
authors.initials: M
authors.lastname: Delledonne
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics*; D040901:Proteomics; D026403:S-Nitrosothiols / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D026403:S-Nitrosothiols; D009569:Nitric Oxide