Gold nanohole array substrates as immunobiosensors.
A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody-gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.
Sharpe
John C
JC
The Horticulture and Food Research Institute of New Zealand, East Street, Private Bag 3123, Hamilton 3240, New Zealand. jsharpe@hortresearch.co.nz
Mitchell
John S
JS
Lin
Ling
L
Sedoglavich
Nemanya
N
Blaikie
Richard J
RJ
D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006854:Hydrocortisone / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D049329:Nanostructures
D000911:Antibodies, Monoclonal; D006046:Gold; D006854:Hydrocortisone