title: Pyrazinamide resistance and pncA gene mutations in Mycobacterium tuberculosis.
abstract: Thirty-four pyrazinamide-resistant and 37 pyrazinamide-susceptible Mycobacterium tuberculosis complex strains were analyzed for pncA gene mutations. None of the sensitive strains had any mutations, apart from silent mutations, whereas all but one resistant strain showed pncA mutations. By using sequencing as a means of early resistance detection, the inconsistency of phenotypic pyrazinamide assays can be circumvented.
authors.affiliation: Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden. pontus.jureen@smi.ki.se
authors.forename: Pontus
authors.initials: P
authors.lastname: Juréen
authors.forename: Jim
authors.initials: J
authors.lastname: Werngren
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Toro
authors.forename: Sven
authors.initials: S
authors.lastname: Hoffner
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000995:Antitubercular Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D004252:DNA Mutational Analysis; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D011718:Pyrazinamide / Q000494:pharmacology*; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics
chemical_list: D000995:Antitubercular Agents; D001426:Bacterial Proteins; D011718:Pyrazinamide; D000581:Amidohydrolases; C487496:PncA protein, Mycobacterium tuberculosis