Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid.
OBJECTIVE
To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or tissue culture medium (TCM) 199.


METHODS
Prospective, randomized, controlled trial.


METHODS
University hospital.


METHODS
Thirteen women undergoing 23 in vitro maturation cycles, from whom 119 oocytes were retrieved.


METHODS
Cumulus-enclosed germinal vesicle-stage oocytes matured in TCM-199-supplemented or HTF-supplemented media.


METHODS
Oocyte maturation and fertilization rates, embryonic developmental quality.


RESULTS
Significant differences were observed between TCM 199 and HTF regarding maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%).


CONCLUSIONS
Human tubal fluid medium, although widely used for embryo fertilization and maintenance in IVF techniques, is not an appropriate medium for the maturation of oocytes obtained from PCOS patients in nonstimulated cycles.
de Araujo
Carlos Henrique Medeiros
CH
Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. cm.araujo@uol.com.br
Nogueira
Daniela
D
de Araujo
Maria Cristina Picinato Medeiros
MC
Martins
Wellington de Paula
Wde P
Ferriani
Rui Alberto
RA
dos Reis
Rosana Maria
RM
D000328:Adult; D018929:Cell Culture Techniques; D002970:Cleavage Stage, Ovum; D003470:Culture Media; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D054315:Oocyte Retrieval; D009865:Oocytes; D009866:Oogenesis; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000503:physiopathology / Q000628:therapy*; D011247:Pregnancy; D011446:Prospective Studies
D003470:Culture Media