Synthesis and photochemical properties of a light-activated fluorophore to label His-tagged proteins.
Rapid and efficient light-induced fluorescence enhancement is demonstrated on a DMNPB-"caged" coumarin derivative carrying a His-tag recognition motif.
Orange
Clélia
C
Universtté Louis Pasteur, CNRS UMR 7175-Laboratoire de chimie bioorganique, Faculté de pharmacie, 74 route du rhin, 67400 Illkirch, France.
Specht
Alexandre
A
Puliti
David
D
Sakr
Elias
E
Furuta
Toshiaki
T
Winsor
Barbara
B
Goeldner
Maurice
M
D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D008027:Light; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*
D005456:Fluorescent Dyes; D011506:Proteins; D006639:Histidine