Dynamic temperature measurement in microfluidic devices using thermochromic liquid crystals.
Thermochromic liquid crystals (TLCs) are used to explore the temperature transients during thermal cycling for microchip-based polymerase chain reaction (PCR). By analyzing the reflected spectra of the TLCs over time, temperature vs. time trajectories were extracted and overshoots/undershoots were estimated. To our knowledge, this is the first report of TLC-based dynamic temperature measurements in a microfluidic device for all PCR temperature stages.
Hoang
Viet N
VN
Applied Miniaturisation Laboratory, Department of Electrical and Computer Engineering, University of Edmonton, AB T6G 2V4, Canada.
Kaigala
Govind V
GV
Backhouse
Christopher J
CJ
D003460:Crystallization; D044085:Microfluidics / Q000295:instrumentation*; D016133:Polymerase Chain Reaction; D013696:Temperature