title: Prolongation of H2 photoproduction by immobilized, sulfur-limited Chlamydomonas reinhardtii cultures.
abstract: Two approaches to prolong the duration of hydrogen production by immobilized, sulfur-limited Chlamydomonas reinhardtii cells are examined. The results demonstrate that continuous H2 photoproduction can occur for at least 90 days under constant flow of TAP medium containing micromolar sulfate concentrations. Furthermore, it is also possible to prolong the duration of H2 production by cycling immobilized cells between minus and plus sulfate conditions.
authors.affiliation: Institute of Basic Biological Problems RAS, Pushchino, Moscow Region 142290, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Laurinavichene
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Kosourov
authors.forename: Maria L
authors.initials: ML
authors.lastname: Ghirardi
authors.forename: Michael
authors.initials: M
authors.lastname: Seibert
authors.forename: Anatoly A
authors.initials: AA
authors.lastname: Tsygankov
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D018914:Cells, Immobilized; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D006859:Hydrogen / Q000032:analysis / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D013455:Sulfur / Q000008:administration & dosage*
chemical_list: D003470:Culture Media; D013455:Sulfur; D006859:Hydrogen