title: [Bone marrow immunophenotyping for the diagnosis of multiple myeloma: practical aspects].
abstract: Multiple myeloma is a malignant proliferative disease of plasma cells. Flow cytometric immunophenotyping makes it possible to identify a malignant clone of myeloma cells in the shortest possible time, to determine its phenotype, and differentiate transformed and preserved plasma cells. The article presents an immunophenotyping strategy using three-color monoclonal antibodies (CD35, CD14, CD38, CD138, and CD19) and an algorithm of verification of transformed plasma cells. Particular emphasis is placed on both the practical aspects of performing this assay and on the clinical application of the obtained results.
authors.forename: A V
authors.initials: AV
authors.lastname: Kurtova
authors.forename: E B
authors.initials: EB
authors.lastname: Sivachenko
authors.forename: L A
authors.initials: LA
authors.lastname: Utkina
authors.forename: K Iu
authors.initials: KIu
authors.lastname: Slobodniuk
authors.forename: N V
authors.initials: NV
authors.lastname: Stepanova
authors.forename: M A
authors.initials: MA
authors.lastname: Iakubovich
authors.forename: S I
authors.initials: SI
authors.lastname: Moiseev
authors.forename: E E
authors.initials: EE
authors.lastname: Zueva
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D009101:Multiple Myeloma / Q000175:diagnosis* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD