Functional characterization of the Aspergillus fumigatus CRZ1 homologue, CrzA.
The protein phosphatase calcineurin is an important mediator connecting calcium-dependent signalling to various cellular responses in multiple organisms. In fungi calcineurin acts largely through regulating Crz1p-like transcription factors. Here we characterize an Aspergillus fumigatus CRZ1 homologue, CrzA and demonstrate its mediation of cellular tolerance to increased concentrations of calcium and manganese. In addition to acute sensitivity to these ions, and decreased conidiation, the crzA null mutant suffers altered expression of calcium transporter mRNAs under high concentrations of calcium, and loss of virulence when compared with the corresponding complemented and wild-type strains. We use multiple expression analyses to probe the transcriptional basis of A. fumigatus calcium tolerance identifying several genes having calA and/or crzA dependent mRNA accumulation patterns. We also demonstrate that contrary to previous findings, the gene encoding the Aspergillus nidulans calcineurin subunit homologue, cnaA, is not essential and that the cnaA deletion mutant shares the morphological phenotypes observed in the corresponding A. fumigatus mutant, DeltacalA. Exploiting the A. nidulans model system, we have linked calcineurin activity with asexual developmental induction, finding that CrzA supports appropriate developmental induction in a calcineurin and brlA-dependent manner in both species.
Soriani
Frederico Marianetti
FM
Faculdade de Ciências Farmacêuticas de Ribeirão PretoUniversidade de São Paulo, São Paulo, Brazil.
Malavazi
Iran
I
da Silva Ferreira
Márcia Eliana
ME
Savoldi
Marcela
M
Von Zeska Kress
Marcia Regina
MR
de Souza Goldman
Maria Helena
MH
Loss
Omar
O
Bignell
Elaine
E
Goldman
Gustavo Henrique
GH
D001232:Aspergillus fumigatus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019703:Calcineurin / Q000235:genetics* / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008345:Manganese / Q000494:pharmacology; D018613:Microscopy, Confocal; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005656:Fungal Proteins; D012333:RNA, Messenger; D008345:Manganese; D019703:Calcineurin; D002122:Calcium Chloride