Preservation injury of the small bowel graft in clinical small bowel transplantation.
Clinical success of small bowel transplantation depends on quality of the preservation small bowel graft which is notoriously sensitive to ischemia. There is still no general agreement as to which segment of the small bowel is preferred (jejunum or ileum) for clinical use. In our study, using a light microscopy and concentrations of tissue serotonin-positive cells, we tried to identify a part of the human intestine, which is more resistant to preservation injury sustained by HTK preservation solution with 1-24 hr of cold ischemia. Statistical analysis of both parameters did not reveal any significant differences between the jejunum and ileum. According to our data, there is no difference between jejunal and ileal grafts in susceptibility to ischemic injury due to cold ischemia within 24 hours when using HTK preservation solution. A significant difference was observed in histological pictures only after 12-hour of cold ischemia in both groups (jejunum and ileum) (Fig. 2, Ref. 11). Full Text (Free, PDF) www.bmj.sk.
Balaz
P
P
Transplant Surgery Department, Institute of Clinical and Experimental Medicine (IKEM), Prague, Czech Republic. peter.balaz@ikem.cz
Kudla
M
M
Lodererova
A
A
Oliverius
M
M
Adamec
M
M
D000328:Adult; D000368:Aged; D050377:Cold Ischemia; D005947:Glucose; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D007511:Ischemia / Q000473:pathology; D008353:Mannitol; D008875:Middle Aged; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011189:Potassium Chloride; D011343:Procaine; D012701:Serotonin / Q000378:metabolism
C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; D012701:Serotonin; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose