Levosimendan effect on spinal cord ischemia-reperfusion injury following aortic clamping.
OBJECTIVE
The new calcium sensitizer, levosimendan, not only acts as a positive inotropic agent but also, vasodilates both venules and arterioles. The aim of this experimental study was to investigate whether levosimendan has protective effects on spinal cord ischemia-reperfusion injury.


METHODS
Twelve New Zealand rabbits were enrolled in this study. In addition to the control group, levosimendan is administered to the experimental group with a loading dose of 12 microg/kg prior to ischemia over a 10-minute period, followed by an infusion of 0.2 microg/kg/min during the ischemia period (30-minutes). Following the neurologic evaluation at the 24th hour of reperfusion, lumbar spinal cords were removed in order to perform microscopic examination and malondialdehyde (MDA) and myeloperoxidase (MPO) measurements.


RESULTS
The mean Tarlov score of the levosimendan group (3.25) was higher than the control group (0.7) (p< 0.05). MDA level was found significantly lower in the levosimendan group when compared with the control group as 1.6 +/- 0.4 nmol/gr and 189.3 +/- 43.6 nmol/gr respectively (p < 0.05). MPO level was also found statistically significant when we compared levosimendan group with the control group. It was calculated as 11.3 +/- 1.0 micro/gr tissue and 39.1 +/- 16.9 micro/gr in the levosimendan and the control groups (p< 0.05). Light microscopic examination was carried out with tissue samples in the 24th hour of the reperfusion. Levosimendan group had better preservation with the microscopic appearance with respect to the control group.


CONCLUSIONS
Levosimendan exhibits an important protection by means of neurological outcome, histopathological, and biochemical analysis for the ischemia-reperfusion injury of the spinal cord following the aortic clamping.
Katircioglu
S Fehmi
SF
Cardiovascular Surgery Clinic, Turkiye Yuksek Ihtisas Hospital, Ankara, Turkey. fehmiege@yahoo.com
Seren
Mustafa
M
Parlar
A Ihsan
AI
Turan
Nilufer N
NN
Manavbasi
Yasemin
Y
Aydog
Gulden
G
Cicekcioglu
Ferit
F
Tutun
Ufuk
U
Ulus
A Tulga
AT
D000818:Animals; D001011:Aorta; D003250:Constriction; D004195:Disease Models, Animal; D006835:Hydrazones / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D008955:Models, Cardiovascular; D009195:Peroxidase / Q000378:metabolism; D011724:Pyridazines / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012107:Research Design; D000077464:Simendan; D016037:Single-Blind Method; D020760:Spinal Cord Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018709:Statistics, Nonparametric; D016896:Treatment Outcome; D014665:Vasodilator Agents / Q000494:pharmacology*
D006835:Hydrazones; D011724:Pyridazines; D014665:Vasodilator Agents; D000077464:Simendan; D008315:Malondialdehyde; D009195:Peroxidase