title: Increasing voltage gradient electrophoresis of DNA.
abstract: We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.
authors.affiliation: Istituto di Biomedicina e Immunologia Molecolare del CNR, Palermo, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Oliva
authors.forename: Sabina
authors.initials: S
authors.lastname: Di Maio
authors.forename: Giorgia
authors.initials: G
authors.lastname: Sisino
authors.forename: Daniele
authors.initials: D
authors.lastname: Bellavia
authors.forename: Rainer
authors.initials: R
authors.lastname: Barbieri
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*
chemical_list: D004272:DNA, Mitochondrial; D004247:DNA