title: Gold nanohole array substrates as immunobiosensors.
abstract: A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody-gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.
authors.affiliation: The Horticulture and Food Research Institute of New Zealand, East Street, Private Bag 3123, Hamilton 3240, New Zealand. jsharpe@hortresearch.co.nz
authors.forename: John C
authors.initials: JC
authors.lastname: Sharpe
authors.forename: John S
authors.initials: JS
authors.lastname: Mitchell
authors.forename: Ling
authors.initials: L
authors.lastname: Lin
authors.forename: Nemanya
authors.initials: N
authors.lastname: Sedoglavich
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Blaikie
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques; D006046:Gold / Q000737:chemistry*; D006854:Hydrocortisone / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D049329:Nanostructures
chemical_list: D000911:Antibodies, Monoclonal; D006046:Gold; D006854:Hydrocortisone