title: [Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae.
  
  
  METHODS
  The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection.
  
  
  RESULTS
  The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively.
  
  
  CONCLUSIONS
  The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wang
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Song
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhu
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Gao
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Lin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*
chemical_list: D012503:Saponins; C533461:loniceroside A; C533462:loniceroside C