Reaping the Benefits of SAGE.
Serial analysis of gene expression (SAGE) is a powerful technique which yields a digital measure of gene expression through the sequencing of libraries of specific mRNA-derived fragments, namely SAGE tags. This chapter introduces the methods and software tools that are available for researchers to analyze gene expression through SAGE analysis. A detailed examination of SAGE analysis in Arabidopsis thaliana using the publicly available analysis tool, SaskSAGE, is provided. The use of this software allows the user to maximize the information gained from SAGE experiments in a model system with a fully sequenced genome.
Robinson
Stephen J
SJ
Agriculture and Agri-Food, Saskatoon Research Centre, Saskatoon, Saskatchewan, Canada.
Guenther
Justin D
JD
Lewis
Christopher T
CT
Links
Matthew G
MG
Parkin
Isobel A P
IA
D017360:Arabidopsis / Q000235:genetics; D030541:Databases, Genetic; D020869:Gene Expression Profiling / Q000379:methods*; D018745:Genome, Plant / Q000235:genetics; D016247:Information Storage and Retrieval; D012984:Software