[Study on chemical constituents from rhizomes of Actaea asiatica].
OBJECTIVE
To investigate the chemical constituents of the rhizomes of Actaea asiatica in order to obtain a more comprehensive understanding of its effective components.


METHODS
Compounds were separated by silica gel chromatography, RP-C18 chromatography and semi-preparative high performance liquid chromatography, and their structures were established by spectral analysis and chemical evidence.


RESULTS
Six compounds were isolated from the ethyl acetate extract. Their structures were identified as 25-O-acetylcimigenol (1), 12beta-hydroxycimigenol (2), 23-epi-26-deoxyactein (3), 27-deoxyacetylacteol (4), 26-deoxycimicifugenin (5) and beta-sitosterol (6).


CONCLUSIONS
All these compounds mentioned above were isolated from the plant for the first time.
Gao
Jing-Chun
JC
Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100094, China.
Zhang
Jin-Chao
JC
Chen
Yao
Y
Yang
Meng-Su
MS
Xiao
Pei-Gen
PG
D031842:Actaea / Q000737:chemistry*; D007810:Lanosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
D012503:Saponins; D012855:Sitosterols; D014315:Triterpenes; D007810:Lanosterol; C457248:23-epi-26-deoxyactein; C025473:gamma-sitosterol