title: Synthesis, GC selective DNA binding and topoisomerase II inhibition activities of ruthenium(II) polypyridyl complex containing 11-aminopteridino[6,7-f][1,10]phenanthrolin-13(12H)-one.
abstract: A DNA-intercalating Ru(II) polypyridyl complex [Ru(bpy)2(appo)]2+ (bpy=2,2'-bipyridine, appo=11-aminopteridino[6,7-f][1,10]phenanthrolin-13(12H)-one) has been synthesized and characterized by elemental analysis, electrospray mass spectra, (1)H NMR, UV/Vis spectrum, fluorescent spectrum and electrochemistry. The DNA-binding, photocleavage, and topoisomerase inhibition of the complex was studied. Interestingly, the complex binds to DNA via an intercalative mode with preference for GC sequences and cleaves the pBR322 DNA upon irradiation. In addition, the complex shows high inhibition activity against topoisomerase II by interfere the DNA religation.
authors.affiliation: MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Hui
authors.initials: H
authors.lastname: Chao
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Yuan-Fang
authors.initials: YF
authors.lastname: Wei
authors.forename: Liang-Nian
authors.initials: LN
authors.lastname: Ji
mesh_terms: D004247:DNA / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D006358:Hot Temperature; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D008163:Luminescent Measurements; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D059005:Topoisomerase II Inhibitors
chemical_list: D004791:Enzyme Inhibitors; D007364:Intercalating Agents; D009942:Organometallic Compounds; D059005:Topoisomerase II Inhibitors; C530401:bis(2,2'-bipyridine)(11-aminopteridine(6,7-f)(1,10)phenanthrolin-13(121H)-one)ruthenium(II); D004247:DNA