Effects of delta-opioid agonist SNC80 on white matter injury following spinal cord ischemia in normothermic and mildly hypothermic rats.
OBJECTIVE
Although the delta-opioid agonist SNC80 has been shown to attenuate hind-limb motor function and gray matter injury in normothermic rats subjected to spinal cord ischemia (SCI), its effects on white matter injury remain undetermined. In the present study, we investigated whether SNC80 could attenuate white matter injury in normothermic and mildly hypothermic rats.


METHODS
Forty rats were randomly allocated to one of following five groups: vehicle or SNC80 with 10 min of SCI at 38 degrees C (V-38-10m or SNC-38-10m, respectively), vehicle or SNC80 with 22 min of SCI at 35 degrees C (V-35-22m or SNC-35-22m, respectively), or sham. SNC80 or vehicle was intrathecally administered 15 min before SCI. Forty-eight hours after reperfusion, the white matter injury was evaluated by the extent of vacuolation.


RESULTS
The percent area of vacuolation in the ventral white matter was significantly lower in the SNC-38-10m and SNC-35-22m groups compared with that in the V-38-10m and V-35-22m groups, respectively (P < 0.05).


CONCLUSIONS
The results indicate that intrathecal treatment with the delta-opioid agonist SNC80 can attenuate the ventral white matter injury following SCI in rats under normothermic and mildly hypothermic conditions.
Horiuchi
Toshinori
T
Department of Anesthesiology, Nara Medical University, Kashihara, Nara, Japan.
Kawaguchi
Masahiko
M
Kurita
Naoko
N
Inoue
Satoki
S
Sakamoto
Takanori
T
Nakamura
Mitsutoshi
M
Konishi
Noboru
N
Furuya
Hitoshi
H
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D001831:Body Temperature / Q000502:physiology; D004195:Disease Models, Animal; D006614:Hindlimb / Q000502:physiology; D007036:Hypothermia, Induced; D007278:Injections, Spinal; D008297:Male; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D010879:Piperazines / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017465:Receptors, Opioid, delta / Q000819:agonists*; D016037:Single-Blind Method; D020760:Spinal Cord Ischemia / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome
D001549:Benzamides; D010879:Piperazines; D017465:Receptors, Opioid, delta; C088464:4-(alpha-(4-allyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl)-N,N-diethylbenzamide