title: In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography.
abstract: We present in vivo experiments conducted with a new fluorescence diffuse optical tomographic (fDOT) system on cancerous mice bearing mammary murine tumors. We first briefly present this new system that has been developed and its associated reconstruction method. Its main specificity is its ability to reconstruct the fluorescence yield even in heterogeneous and highly attenuating body regions such as lungs and to enable mouse inspection without immersion in optical index matching liquid (Intralipid and ink). Some phantom experiments validate the performance of this new system for heterogeneous media inspection. Its use for a mice study is then related. It consists in the follow-up of the lungs at different stages of tumor development after injection of RAFT-(cRGD)4-Alexa700. As expected, the reconstructed fluorescence increases along with the tumor stage. These results validate the use of our system for biological studies of small animals.
authors.affiliation: Commissariat à l'Energie Atomique, Lab. d'Electronique de Technologie de l'Information, MINATEC, 17 rue des Martyrs, 38054 Grenoble, Cedex 9, France. anne.koenig@cea.fr
authors.forename: Anne
authors.initials: A
authors.lastname: Koenig
authors.forename: Lionel
authors.initials: L
authors.lastname: Hervé
authors.forename: Véronique
authors.initials: V
authors.lastname: Josserand
authors.forename: Michel
authors.initials: M
authors.lastname: Berger
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boutet
authors.forename: Anabela
authors.initials: A
authors.lastname: Da Silva
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Dinten
authors.forename: Philippe
authors.initials: P
authors.lastname: Peltié
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Coll
authors.forename: Philippe
authors.initials: P
authors.lastname: Rizo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods