Screening and investigation of dye decolorization activities of basidiomycetes.
The decolorization of industrial dyes was investigated using extracellular enzymes produced by 21 basidiomycetes, mainly edible mushrooms. Among the 27 dyes used in this study, nine were decolorized by over 40%. Most fungi decolorized Acid Orange 20, but they showed different specificities in the case of the other dyes. Determination of activity staining by native polyacrylamide gel electrophoresis revealed that all the decolorization activities corresponded to 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) oxidation activities.
Nozaki
Kouichi
K
Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, Nagano, Japan. knoza@shinshu-u.ac.jp
Beh
Chee Hai
CH
Mizuno
Masahiro
M
Isobe
Tetsuhiro
T
Shiroishi
Masahiro
M
Kanda
Takahisa
T
Amano
Yoshihiko
Y
D001487:Basidiomycota / Q000201:enzymology* / Q000302:isolation & purification*; D052160:Benzothiazoles / Q000737:chemistry; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification; D013451:Sulfonic Acids / Q000737:chemistry
D052160:Benzothiazoles; D004396:Coloring Agents; D005656:Fungal Proteins; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D010088:Oxidoreductases