Differential cross-reactivity of monoclonal antibody OPD4 (anti-CD45RO) in macaques.
Immunologic research in nonhuman primates is occasionally limited by the availability of reagents that cross-react in nonhuman primates. One major limitation has been the lack of a monoclonal antibody to CD45RO. Although the monoclonal antibody UCHL-1 is used to detect CD45RO isoforms in humans, it does not react with nonhuman primates, mandating the use of alternative strategies to define "memory" T cell responses in nonhuman primates. The current study examined the reactivity and specificity of another antibody against CD45RO, clone OPD4, in macaques. Here we demonstrate that OPD4 specifically labels memory CD4+ T cells in approximately 44% of rhesus macaques (Macaca mulatta) of Indian but not Chinese origin. In contrast, tissues from pigtail macaques (Macaca nemestrina) react with this clone, indicating that OPD4 may be useful for examining memory CD4+ T cells in certain macaques, but its utility may be limited in other species or even among individual macaques.
Wang
Xiaolei
X
Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University Health Sciences Center, 18703 Three Rivers Road, Covington, LA 70433, USA.
Pahar
Bapi
B
Rasmussen
Terri
T
Alvarez
Xavier
X
Dufour
Jason
J
Rasmussen
Kelsi
K
Lackner
Andrew A
AA
Veazey
Ronald S
RS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008251:Macaca / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017493:Leukocyte Common Antigens