Microsatellite markers from the Chagas disease vector, Rhodnius prolixus (Hemiptera, Reduviidae), and their applicability to Rhodnius species.
Ten microsatellites were isolated and characterized from a partial genomic library of Rhodnius prolixus, the principal Chagas disease vector in Venezuela, Colombia and Central America. These polymorphic molecular markers could be particularly useful in Chagas disease control initiatives. A wider applicability of the primer-pairs isolated was shown, from 6 to 10 loci being amplifiable in five out of the ten Rhodnius species tested, namely R. domesticus, R. nasutus, R. neglectus, R. neivai and R. robustus. Interestingly, all the loci were amplified in the latter. These markers may be of interest to trace the colonization of human dwellings from triatomine sylvatic populations in order to better define epidemiological risk patterns.
Harry
M
M
UMR 137, UFR de Sciences, Université Paris 12, Creteil, France. harry@univ-paris12.fr
Roose
C L
CL
Vautrin
D
D
Noireau
F
F
Romaña
C A
CA
Solignac
M
M
D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D004199:Disease Vectors; D015723:Gene Library; D005843:Geography; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D012239:Rhodnius / Q000235:genetics* / Q000469:parasitology; D013045:Species Specificity