Copper(II) complexes of lipophilic aminoglycoside derivatives for the amino acid enantiomeric separation by ligand-exchange liquid chromatography.
In this paper, a new class of ligand-exchange chiral stationary phase (LE-CSP) based on the copper complexes of lipophilic aminoglycoside derivatives was reported. Different stationary phases were developed by coating reversed-phase liquid chromatography supports with three neamine derivatives carrying a lipophilic octadecyl chain at the 4', 5 and 6 positions, respectively. The enantioselective ability of these LE neamine-based CSPs was evaluated and the 4'-derivative coated column was found to be the most interesting one for the amino acid resolution. The effects of the variation of several chromatographic parameters on the enantioseparation were evaluated in order to identify the analysis optimal conditions.
Zaher
Mustapha
M
Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Grenoble I (Joseph Fourier), UFR de Pharmacie, 38041 Grenoble Cedex 9, France.
Baussanne
Isabelle
I
Ravelet
Corinne
C
Halder
Somnath
S
Haroun
Mohamed
M
Fize
Jennifer
J
Décout
Jean-Luc
JL
Peyrin
Eric
E
D000596:Amino Acids / Q000032:analysis*; D000617:Aminoglycosides / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D003300:Copper / Q000737:chemistry; D013237:Stereoisomerism
D000596:Amino Acids; D000617:Aminoglycosides; D003300:Copper