[Effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells].
OBJECTIVE
To study the effect of luteolin on COX-2 and mPGES-1 expression in LPS-induced RAW264.7 cells.


METHODS
After being pretreated with different concentrations of luteolin for 30 min, and then incubated with 1 microg/ml LPS for 12h, the effect of luteolin on the product of PGE2 in RAW264.7 cells was measured by enzyme immunoassay (EIA). The mRNA expression of COX-2 and mPGES-1 in RAW264.7 cells were analysed by RT-PCR. The COX-2 and mPGES-1 protein expression in RAW264. 7 cells were analysed by western blotting.


RESULTS
Luteolin inhibited the LPS-induced PGE, synthesis in RAW264.7 cells. The mRNA and protein expression of COX-2 and mPGES-1 in RAW264.7 cells were also decreased by luteolin.


CONCLUSIONS
Luteolin can inhibit significantly the expression of COX-2 and mPGES-1 in PGE2 synthetic pathway.
Wang
Xu-guang
XG
First Affiliated Hospital of Guangdong Medical College, Zhanjiang 524100, China. marvin009@126.com
Chen
Gen-yin
GY
Chen
Miao-ping
MP
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D047311:Luteolin / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D000071876:Prostaglandin-E Synthases; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; C000605865:Ptges protein, mouse; D015232:Dinoprostone; D047311:Luteolin