Free copper, ferroxidase and SOD1 activities, lipid peroxidation and NO(x) content in the CSF. A different marker profile in four neurodegenerative diseases.
The understanding of oxidative damage in different neurodegenerative diseases could enhance therapeutic strategies. Our objective was to quantify lipoperoxidation and other oxidative products as well as the activity of antioxidant enzymes and cofactors in cerebrospinal fluid (CSF) samples. We recorded data from all new patients with a diagnosis of either one of the four most frequent neurodegenerative diseases: Parkinson's disease (PD), Alzheimer's disease (AD), Huntington's disease (HD) and lateral amyotrophic sclerosis (ALS). The sum of nitrites and nitrates as end products of nitric oxide (NO) were increased in the four degenerative diseases and fluorescent lipoperoxidation products in three (excepting ALS). A decreased Cu/Zn-dependent superoxide dismutase (SOD) activity characterized the four diseases. A significantly decreased ferroxidase activity was found in PD, HD and AD, agreeing with findings of iron deposition in these entities, while free copper was found to be increased in CSF and appeared to be a good biomarker of PD.
Boll
Marie-Catherine
MC
Department of Clinical Investigation in Neurosciences, National Institute of Neurology and Neurosurgery, Mexico, DF, Mexico.
Alcaraz-Zubeldia
Mireya
M
Montes
Sergio
S
Rios
Camilo
C
D000544:Alzheimer Disease / Q000134:cerebrospinal fluid; D000690:Amyotrophic Lateral Sclerosis / Q000134:cerebrospinal fluid; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000134:cerebrospinal fluid*; D002570:Ceruloplasmin / Q000134:cerebrospinal fluid*; D003300:Copper / Q000134:cerebrospinal fluid*; D006801:Humans; D006816:Huntington Disease / Q000134:cerebrospinal fluid; D015227:Lipid Peroxidation; D019636:Neurodegenerative Diseases / Q000134:cerebrospinal fluid*; D009569:Nitric Oxide / Q000134:cerebrospinal fluid*; D010300:Parkinson Disease / Q000134:cerebrospinal fluid; D012372:ROC Curve; D013482:Superoxide Dismutase / Q000134:cerebrospinal fluid*; D000072105:Superoxide Dismutase-1
D000975:Antioxidants; D015415:Biomarkers; C000606290:SOD1 protein, human; D009569:Nitric Oxide; D003300:Copper; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D002570:Ceruloplasmin