Gamma delta+ T cells involvement in viral immune control of chronic human herpesvirus 8 infection.
Little is known about what effector populations are associated with the control of human herpesvirus 8 (HHV-8) infection in vivo. We compared T lymphocyte subsets among HIV-HHV-8+ and HIV-HHV-8- infected human individuals. alphabeta+ T cells from HHV-8-infected individuals displayed a significantly higher percentage of differentiated effector cells among both CD4+ and CD8+ T cell subsets. HHV-8 infection was associated with significant expansion of gammadelta+ Vdelta1 T cells expressing a differentiated effector cell phenotype in peripheral blood. In vitro stimulation of PBMC from HHV-8-infected individuals with either infectious viral particles or different HHV-8 viral proteins resulted in gammadelta Vdelta1 T cell activation. In addition, gammadelta Vdelta1 T cells displayed a strong reactivity against HHV-8-infected cell lines and prevented the release of infectious viral particles following the induction of lyric replication. These data indicate that gammadelta T cells play a role in both innate and adaptive T cell responses against HHV-8 in immunocompetent individuals.
Barcy
Serge
S
Department of Laboratory Medicine, University of Washington, Seattle, WA 98109, USA. sbarcy@u.washington.edu
De Rosa
Stephen C
SC
Vieira
Jeffrey
J
Diem
Kurt
K
Ikoma
Minako
M
Casper
Corey
C
Corey
Lawrence
L
D000328:Adult; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism; D001483:Base Sequence; D002454:Cell Differentiation / Q000276:immunology; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D002908:Chronic Disease; D002999:Clone Cells; D006566:Herpesviridae Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D014709:Vero Cells
D000998:Antiviral Agents; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma