title: HN protein of Newcastle disease virus causes apoptosis in chicken embryo fibroblast cells.
abstract: Newcastle disease virus (NDV), an avian paramyxovirus, induces apoptosis in chicken embryo fibroblast (CEF) cells. In the present investigation, the ability of haemagglutinin-neuraminidase (HN) protein of NDV to cause apoptosis in CEF cells was examined. The results revealed that cells expressing the HN protein demonstrated decreased DNA content, phosphatidylserine exposure and increased cytoplasmic vacuolation. Up-regulation of caspase-1, -9, -8, -3, loss of mitochondrial transmembrane potential and an increase in oxidative stress were also observed in cells expressing the HN protein. Based on the above results it can be concluded that HN protein of NDV causes apoptosis in CEF cells.
authors.affiliation: Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Ravindra
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Tiwari
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sharma
authors.forename: Yogendra Singh
authors.initials: YS
authors.lastname: Rajawat
authors.forename: Barkha
authors.initials: B
authors.lastname: Ratta
authors.forename: Sudesh
authors.initials: S
authors.lastname: Palia
authors.forename: N R
authors.initials: NR
authors.lastname: Sundaresan
authors.forename: Uttara
authors.initials: U
authors.lastname: Chaturvedi
authors.forename: Arunakumar
authors.initials: A
authors.lastname: Gangaplara
authors.forename: G B Aruna
authors.initials: GB
authors.lastname: Kumar
authors.forename: Kantaraja
authors.initials: K
authors.lastname: Chindera
authors.forename: Meeta
authors.initials: M
authors.lastname: Saxena
authors.forename: P K
authors.initials: PK
authors.lastname: Subudhi
authors.forename: Anant
authors.initials: A
authors.lastname: Rai
authors.forename: R S
authors.initials: RS
authors.lastname: Chauhan
mesh_terms: D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D015707:HN Protein / Q000378:metabolism*; D009522:Newcastle disease virus / Q000378:metabolism / Q000472:pathogenicity*; D018384:Oxidative Stress; D010718:Phosphatidylserines / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D015707:HN Protein; D010718:Phosphatidylserines; D020169:Caspases