[Studies on hydrolysis of aconitine].
OBJECTIVE
To find out hydrolysis regularity of aconitine.


METHODS
Aconitine was air-tighted and hydrolyzed in water for 8, 12, 16, 20, 24 h. The hydrolysis products were analyzed by HPLC-MS. A major hydrolysis product was isolated by alumina oxide column chromatography, and identified by 1H and 13C-NMR spectra.


RESULTS
HPLC-MS analysis shows that the major hydrolysis products are benzoylaconine and aconine. The hydrolysis can mostly be completed within 20 hours.


CONCLUSIONS
Diester aconitum alkaloid can be changed to monoester aconitum and aconine alkaloids. Under the controled condition benzoylaconine is a major hydrolysis products.
Huang
Qin-An
QA
Chemistry Department of Wannan Medical College, Wuhu 241001, China. hqaa128@sohu.com
Zhang
Yu-Mei
YM
He
Yi
Y
Lu
Jing
J
Lin
Rui-Chao
RC
D000157:Aconitine / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006868:Hydrolysis; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water / Q000737:chemistry
D014867:Water; C047307:benzoylaconine; C047306:aconine; D000157:Aconitine