Verticillium dahliae toxins-induced nitric oxide production in Arabidopsis is major dependent on nitrate reductase.
The source of nitric oxide (NO) in plants is unclear and it has been reported NO can be produced by nitric oxide synthase (NOS) like enzymes and by nitrate reductase (NR). Here we used wild-type, Atnos1 mutant and nia1, nia2 NR-deficient mutant plants of Arabidopsis thaliana to investigate the potential source of NO production in response to Verticillium dahliae toxins (VD-toxins). The results revealed that NO production is much higher in wild-type and Atnos1 mutant than in nia1, nia2 NR-deficient mutants. The NR inhibitor had a significant effect on VD-toxins-induced NO production; whereas NOS inhibitor had a slight effect. NR activity was significantly implicated in NO production. The results indicated that as NO was induced in response to VD-toxins in Arabidopsis, the major source was the NR pathway. The production of NOS-system appeared to be secondary.
Shi
Fu-Mei
FM
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Li
Ying-Zhang
YZ
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D009183:Mycotoxins / Q000494:pharmacology*; D050901:Nitrate Reductase / Q000187:drug effects / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D030821:Plants, Genetically Modified; D020172:Verticillium / Q000737:chemistry*
D009183:Mycotoxins; D009569:Nitric Oxide; D050901:Nitrate Reductase