Activity and selectivity of W110A secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus in organic solvents and ionic liquids: mono- and biphasic media.
The asymmetric reduction of hydrophobic phenyl-ring-containing ketones and the enantiospecific kinetic resolution of the corresponding racemic alcohols catalyzed by Thermoanaerobacter ethanolicus W110A secondary alcohol dehydrogenase were performed in mono- and biphasic systems containing either organic solvents or ionic liquids. Both yield and enantioselectivity for these transformations can be controlled by changing the reaction medium. The enzyme showed high tolerance to both water-miscible and -immiscible solvents, which allows biotransformations to be conducted at high substrate concentrations.
Musa
Musa M
MM
Department of Chemistry, University of Georgia, Athens, GA 30602-2556, USA.
Ziegelmann-Fjeld
Karla I
KI
Vieille
Claire
C
Phillips
Robert S
RS
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000438:Alcohols / Q000138:chemical synthesis / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001881:Borates / Q000737:chemistry*; D002384:Catalysis; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry; D010084:Oxidation-Reduction; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013449:Sulfonamides / Q000737:chemistry*; D046968:Thermoanaerobacter / Q000201:enzymology*; D014867:Water / Q000737:chemistry
C493485:1-butyl-3-methylimidazolium bis((trifluoromethyl)sulfonyl)amide; C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D000438:Alcohols; D001426:Bacterial Proteins; D001881:Borates; D007093:Imidazoles; D052578:Ionic Liquids; D007659:Ketones; D012997:Solvents; D013449:Sulfonamides; D014867:Water; D000429:Alcohol Oxidoreductases