Mitochondrial division in Caenorhabditis elegans.
The study of mitochondrial division proteins has largely focused on yeast and mammalian cells. We describe methods to use Caenorhabditis elegans as an alternative model for studying mitochondrial division, taking advantage of the many wonderful resources provided by the C. elegans community. Our methods are largely based on manipulation of gene expression using classic and molecular genetic techniques combined with fluorescence microscopy. Some biochemical methods are also included. As antibodies become available, these biochemical methods are likely to become more sophisticated.
Gandre
Shilpa
S
Department of Biological Chemistry, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
van der Bliek
Alexander M
AM
D000818:Animals; D030801:Animals, Genetically Modified; D001426:Bacterial Proteins / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000166:cytology / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000302:isolation & purification; D002458:Cell Fractionation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D034622:RNA Interference
D001426:Bacterial Proteins; D029742:Caenorhabditis elegans Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D004247:DNA