Elementary processes in cells after light absorption do not depend on the degree of polarization: implications for the mechanisms of laser phototherapy.
OBJECTIVE
The objective of this work was to evaluate the importance of the degree of light polarization in stimulation of cellular metabolism.


BACKGROUND
Although the possible role of polarization's effects on the mechanisms of laser phototherapy is sometimes discussed in the literature, there are still no clear answers.


METHODS
A model system (HeLa cell suspension) was used in which the lengths of light scattering (l sc) and absorption (l a) were much larger than the thickness of the irradiated layer (L = 3 mm). The cell suspension (1 x 10(6) cells/cm3) was irradiated with a diode laser (lambda = 637 nm, D = 65.7 J/m2, tau = 10 sec, I = 6.57 W/m2). The polarization degree (99.4%, 60.9%, and 34.2%) of the beam was changed by means of optical fibers of different lengths. The irradiated suspension was incubated at 37 degrees C for 30 min, and the attached cells were counted afterwards.


RESULTS
The cell fraction stimulated to adhere by red light at 637 nm was nearly the same in all three experimental groups (58.1% +/- 2.5%, 57.6% +/- 3.5%, and 62.5% +/- 3.2% for degree of beam polarization of 99.4%, 66.9%, and 34.2%, respectively). There was no statistically significant difference in these results (p <0.8, <0.6, and <0.7, respectively). At the same time, all three groups had statistically significant differences (p < 0.01) in adherence from the sham-irradiated control group (39.1% +/- 2.2%).


CONCLUSIONS
The biological effect (stimulation of cell attachment) of light with lambda = 637 nm on cells in our model system was pronounced, but did not depend on the degree of light polarization. Elementary processes in cells (light absorption and photochemistry) do not appear to depend on the degree of light polarization.
Karu
Tiina I
TI
Institute of Laser and Information Technologies of Russian Academy of Sciences, Troitsk, Moscow Region, Russian Federation. tkaru@isan.troitsk.ru
Pyatibrat
Ludmila V
LV
Moskvin
Sergei V
SV
Andreev
Sergei
S
Letokhov
Vladilen S
VS
D002448:Cell Adhesion / Q000528:radiation effects*; D018929:Cell Culture Techniques; D005898:Glass; D006367:HeLa Cells / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor; D008027:Light; D008954:Models, Biological; D010789:Phototherapy / Q000295:instrumentation*; D012542:Scattering, Radiation; D013499:Surface Properties / Q000528:radiation effects