title: Prevalence of virulence genes and cytolethal distending toxin production in Campylobacter jejuni isolates from diarrheal patients in Bangladesh.
abstract: From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 strains were identified as Campylobacter jejuni and 5 as Campylobacter coli. The presence of flaA (100%), cadF (100%), racR (100%), dnaJ (100%), pldA (100%), ciaB (95%), virB11 (0%), ceuE (82.5%), cdtA (97.5%), cdtB (97.5%), cdtC (97.5%), and wlaN (7.5%) genes was detected in C. jejuni by PCR. All C. jejuni strains but one produced cytolethal distending toxin in a HeLa cell assay.
authors.affiliation: Enteric Microbiology Laboratory, Laboratory Sciences Division, ICDDR,B, GPO Box 128, Dhaka 1000, Bangladesh. kaisar@icddrb.org
authors.forename: Kaisar A
authors.initials: KA
authors.lastname: Talukder
authors.forename: Mohammad
authors.initials: M
authors.lastname: Aslam
authors.forename: Zhahirul
authors.initials: Z
authors.lastname: Islam
authors.forename: Ishrat J
authors.initials: IJ
authors.lastname: Azmi
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Dutta
authors.forename: Sabir
authors.initials: S
authors.lastname: Hossain
authors.forename: Alam
authors.initials: A
authors.lastname: Nur-E-Kamal
authors.forename: Gopinath B
authors.initials: GB
authors.lastname: Nair
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cravioto
authors.forename: David A
authors.initials: DA
authors.lastname: Sack
authors.forename: Hubert P
authors.initials: HP
authors.lastname: Endtz
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D001459:Bangladesh / Q000453:epidemiology; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D037521:Virulence Factors; C067044:cytolethal distending toxin