[Effects of 17beta-estradiol on the intracellular calcium of masticatory muscles myoblast in vitro].
OBJECTIVE
To observe the effects of 17beta-estradiol on the intracellular calcium of masticatory muscles myoblast.


METHODS
Myoblasts from maxillofacial skeletal muscle of one week old female Sprague-Dawley rats were cultured. Fluo-4-AM as the Ca2+ indicator and the laser confocal microscope system were used to observe the effects of estrogen on the cytoplasmic Ca2+ concentration in the normal pH condition and the acid condition (pH = 6.7).


RESULTS
In the normal pH condition, when 17beta-estradiol (10(-9), 10(-8), 10(-7) mol/L) were added to cells cytoplasmic Ca2+ immediately increased then decreased right away, and in the end came into a new Ca2+ homeostasis in the base line. In the acid condition, 17beta-estradiol (10(-9), 10(-8), 10(-7) mol/L) made the cytoplasmic Ca2+ decreased immediately then came into a new Ca2+ homeostasis under the base line.


CONCLUSIONS
The results suggest that estrogen may maintain the skeletal cytoplasmic Ca2+ concentration in a lower level and reduce the cytoplasmic Ca2+ accumulation to keep the normal functions of masticatory muscles myoblast.
Wang
Xi-Jun
XJ
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Guo
Hong-Mei
HM
Wang
Yan-Min
YM
Yi
Xin-Zhu
XZ
D000814:Aniline Compounds; D000818:Animals; D002118:Calcium; D004958:Estradiol; D005260:Female; D066298:In Vitro Techniques; D008410:Masticatory Muscles; D032446:Myoblasts; D051381:Rats; D017207:Rats, Sprague-Dawley; D014966:Xanthenes
D000814:Aniline Compounds; C409648:Fluo 4; D014966:Xanthenes; D004958:Estradiol; D002118:Calcium