title: The effect of casein phosphopeptide-amorphous calcium phosphate on remineralization of artificial caries-like lesions: an in vitro study.
abstract: BACKGROUND
  The aims of this study were to investigate the efficacy of CPP-ACP containing Tooth Mousse on the remineralization of enamel lesions and to compare its efficacy to that of a fluoride-containing toothpaste.
  
  
  METHODS
  Permanent teeth were placed in demineralizing solution for 96 hours to produce artificial caries-like lesions 120-200 microm in depth. They were sectioned into 100-150 microm thick samples and randomly assigned to five groups: for Group A, a fluoridated toothpaste (1100 ppm) was used as a positive control and in Group B, a non-fluoridated toothpaste was used as a negative control. Tooth Mousse containing CPP-ACP was tested by three different means: as a toothpaste (Group C); as a topical coating (Group D); and (Group E) as a topical coating after treating the sections with the same fluoridated toothpaste as in Group A.
  
  
  RESULTS
  The lesion depth decreased significantly by 7 per cent in Group A, 10.1 per cent in Groups C and D, and 13.1 per cent in Group E (Paired t- test, p < 0.05), while in Group B the lesion depth increased significantly by 23 per cent.
  
  
  CONCLUSIONS
  Based on the data obtained, CPP-ACP containing Tooth Mousse remineralized initial enamel lesions and it showed a higher remineralizing potential when applied as a topical coating after the use of a fluoridated toothpaste.
authors.affiliation: Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR.
authors.forename: V L N
authors.initials: VL
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Itthagarun
authors.forename: N M
authors.initials: NM
authors.lastname: King
mesh_terms: D000287:Administration, Topical; D002327:Cariostatic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002364:Caseins / Q000008:administration & dosage / Q000627:therapeutic use*; D003731:Dental Caries / Q000473:pathology / Q000628:therapy*; D003743:Dental Enamel / Q000187:drug effects / Q000473:pathology; D005459:Fluorides / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008852:Microradiography; D008859:Microscopy, Polarization; D013997:Time Factors; D014089:Tooth Remineralization / Q000379:methods*; D014100:Toothpastes / Q000627:therapeutic use
chemical_list: D002327:Cariostatic Agents; D002364:Caseins; D014100:Toothpastes; C447032:casein phosphopeptide-amorphous calcium phosphate nanocomplex; D005459:Fluorides