The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability.
Loss or gain of whole chromosome, the form of chromosome instability commonly associated with cancers is thought to arise from aberrant chromosome segregation during cell division. Chromosome segregation in mitosis is orchestrated by the interaction of kinetochores with spindle microtubules. Our studies show that NEK2A is a kinetochore-associated protein kinase essential for faithful chromosome segregation. However, it was unclear how NEK2A ensures accurate chromosome segregation in mitosis. Here we show that NEK2A-mediated Hec1 (highly expressed in cancer) phosphorylation is essential for faithful kinetochore microtubule attachments in mitosis. Using phospho-specific antibody, our studies show that NEK2A phosphorylates Hec1 at Ser165 during mitosis. Although such phosphorylation is not required for assembly of Hec1 to the kinetochore, expression of non-phosphorylatable mutant Hec1(S165) perturbed chromosome congression and resulted in a dramatic increase in microtubule attachment errors, including syntelic and monotelic attachments. Our in vitro reconstitution experiment demonstrated that Hec1 binds to microtubule in low affinity and phosphorylation by NEK2A, which prevents aberrant kinetochore-microtubule connections in vivo, increases the affinity of the Ndc80 complex for microtubules in vitro. Thus, our studies illustrate a novel regulatory mechanism in which NEK2A kinase operates a faithful chromosome attachment to spindle microtubule, which prevents chromosome instability during cell division.
Du
J
J
Hefei National Laboratory for Physical Sciences at Micro-scale, University of Science and Technology of China, Hefei, China.
Cai
X
X
Yao
J
J
Ding
X
X
Wu
Q
Q
Pei
S
S
Jiang
K
K
Zhang
Y
Y
Wang
W
W
Shi
Y
Y
Lai
Y
Y
Shen
J
J
Teng
M
M
Huang
H
H
Fei
Q
Q
Reddy
E S
ES
Zhu
J
J
Jin
C
C
Yao
X
X
D000818:Animals; D002474:Cell-Free System / Q000201:enzymology; D043171:Chromosomal Instability / Q000235:genetics; D020090:Chromosome Segregation / Q000502:physiology*; D003598:Cytoskeletal Proteins; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008870:Microtubules / Q000235:genetics / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D009154:Mutation; D000072379:NIMA-Related Kinases; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D008941:Spindle Apparatus / Q000235:genetics / Q000378:metabolism*
D003598:Cytoskeletal Proteins; C120529:NDC80 protein, human; D009687:Nuclear Proteins; C089441:NEK2 protein, human; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases