[Effect of anti-BPDE on expression of HER2/neu gene in the human bronchial epithelial cell line].
OBJECTIVE
To explore the effects of anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE) on expression of HER2/neu gene in the human bronchial epithelial cells.


METHODS
The levels of HER2/neu mRNA and protein expressions, and its protein localization were examined in the malignant transformed 16HBE (16HBE-T) induced by 2.0 micromol/L anti-BPDE and untransformed control 16HBE (16HBE-N) using semi-quantitative RT-PCR, SYBR Green I Real time quantitative RT-PCR (QRT-PCR), western blot and immunocytochemical method respectively.


RESULTS
The expressions of mRNA and protein of HER2/neu appeared to be a significant increase in 16HBE-T in comparison with those of 16HBE-N detected by several assays. HER2/neu protein showed membrane localization.


CONCLUSIONS
HER2/neu could be overexpressed on treatment of human bronchial epithelial cells with anti-BPDE. The activity of HER2/neu oncogene might play an important role in the malignant transformed 16HBE induced by anti-BPDE.
Fu
Juan
J
Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. fjuangy@126.com
Jiang
Yiguo
Y
Zou
Yunfeng
Y
Shen
Yuelan
Y
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology*; D002273:Carcinogens / Q000633:toxicity*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology*; D018734:Genes, erbB-2 / Q000235:genetics; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D002273:Carcinogens; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human