Determination of total flavonoids in Scutellaria barbata D. Don by dynamic ultrasonic extraction coupled with on-line spectrophotometry.
A dynamic ultrasonic extraction (DUE) coupled with on-line detection by spectrophotometry is proposed for the determination of total flavonoids (calculated against scutellarin) in Scutellaria barbata D. Don. The extraction was performed in a common self-made extraction vessel put in an ultrasonic bath and a peristaltic pump was used to deliver the extraction solvent. Several experimental parameters of DUE, including flow rate and concentration of extraction solvent, ultrasonic power and sample amount, were optimized. In this work an on-line method was provided with the advantages of minimum sample amount (5 mg), on-line monitoring the extraction process and time saving (12.5 min). Compared with off-line method, the proposed method would be more convenient for obtaining continuous measurements and rapid optimization of the extraction process.
Hu
Xiuli
X
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
You
Jingyan
J
Bao
Changli
C
Zhang
Huarong
H
Meng
Xiangzhe
X
Xiao
Tingting
T
Zhang
Kun
K
Wang
Yutang
Y
Wang
Huimin
H
Zhang
Hanqi
H
Yu
Aimin
A
D005419:Flavonoids / Q000032:analysis*; D015203:Reproducibility of Results; D031363:Scutellaria / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014465:Ultrasonics
D005419:Flavonoids; D012997:Solvents