title: The effects of long-duration, low-temperature ground transportation on physiological and biochemical indicators of stress in mice.
abstract: Transportation can cause stress to laboratory animals and alter physiological characteristics that may confound experimental results. The authors investigated stress-related effects of 3-4 h of transportation by truck in two strains of mice (C57BL/6, which are known to be aggressive, and ICR, which are less aggressive). Transported mice had sufficient space and access to water, though temperature in the truck was lower than what is usually recommended. Transportation affected the following parameters in both strains of mice: (i) serum corticosterone concentrations, (ii) expression of the chaperone proteins Hsp70 and Grp78 in various tissues and (iii) concentrations of serological enzymes that are associated with liver disease. These parameters also differed substantially between the two strains of mice.
authors.affiliation: Laboratory Animal Resources Team, National Institute of Toxicological Research, Korea FDA, Seoul 122-704, Korea.
authors.forename: Sun Bo
authors.initials: SB
authors.lastname: Shim
authors.forename: Se Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Chuel Kyu
authors.initials: CK
authors.lastname: Kim
authors.forename: Byoung Guk
authors.initials: BG
authors.lastname: Kim
authors.forename: Yong Kyu
authors.initials: YK
authors.lastname: Kim
authors.forename: Seung Wan
authors.initials: SW
authors.lastname: Jee
authors.forename: Su Hae
authors.initials: SH
authors.lastname: Lee
authors.forename: Ji Soon
authors.initials: JS
authors.lastname: Sin
authors.forename: Chang Joon
authors.initials: CJ
authors.lastname: Bae
authors.forename: Byoung Chun
authors.initials: BC
authors.lastname: Lee
authors.forename: Mee Kyung
authors.initials: MK
authors.lastname: Jang
authors.forename: Jung Sik
authors.initials: JS
authors.lastname: Cho
authors.forename: Kab Ryong
authors.initials: KR
authors.lastname: Chae
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001522:Behavior, Animal; D003080:Cold Temperature / Q000009:adverse effects*; D003345:Corticosterone / Q000097:blood; D000091342:Endoplasmic Reticulum Chaperone BiP; D004777:Environment; D004798:Enzymes / Q000097:blood; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018832:Molecular Chaperones / Q000378:metabolism; D013315:Stress, Psychological / Q000097:blood* / Q000503:physiopathology; D013997:Time Factors; D014186:Transportation
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D004798:Enzymes; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D018832:Molecular Chaperones; D003345:Corticosterone