title: Functional expression of the Cre recombinase in actinomycetes.
abstract: Site-specific recombinases revolutionized "in vivo" genetic engineering because they can catalyze precise excisions, integrations, inversions, or translocations of DNA between their distinct recognition target sites. We have constructed a synthetic gene encoding Cre recombinase with the GC content 67.7% optimized for expression in high-GC bacteria and demonstrated this gene to be functional in Streptomyces lividans. Using the synthetic cre(a) gene, we have removed an apramycin resistance gene flanked by loxP sites from the chromosome of S. lividans with 100% efficiency. Sequencing of the chromosomal DNA part showed that excision of the apramycin cassette by Cre recombinase was specific.
authors.affiliation: Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Freiburg, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Fedoryshyn
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Welle
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
mesh_terms: D003001:Cloning, Molecular; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D048170:Streptomyces lividans / Q000235:genetics* / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases