Molecular evidence of Bartonella DNA in ixodid ticks in Czechia.
We report the development of a novel method for detection of Bartonella DNA in ixodid ticks. The assay is based on a specific amplification of a part of 16S rRNA gene and 16S-23S rRNA intergenic spacer region of Bartonella sp. by nested PCR and Southern blot hybridization with specific DNA probe; the method is highly sensitive and specific. The screening of 327 unfed ticks collected in different urban and suburban areas of Czechia in 2003-2005 revealed the presence of Bartonella DNA in four Ixodes ricinus individuals (1.2%), two males, one female and one nymph.
Hercík
K
K
Laboratory of Cell Signaling, Cell and Molecular Microbiology Division, Institute ofMicrobiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Hásová
V
V
Janecek
J
J
Branny
P
P
D000818:Animals; D001473:Bartonella / Q000235:genetics / Q000302:isolation & purification*; D015139:Blotting, Southern / Q000379:methods*; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D006801:Humans; D026863:Ixodidae / Q000145:classification / Q000382:microbiology*; D008297:Male; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S