Eupatilin protects gastric epithelial cells from oxidative damage and down-regulates genes responsible for the cellular oxidative stress.
OBJECTIVE
The formulated ethanol extract (DA-9601) of Artemisia asiatica has pronounced antiinflammatory activities and exhibits cytoprotective effects against gastrointestinal damage. Here we investigated whether eupatilin, a major component of DA-9601, has a property of antioxidant activity and protects gastric epithelial cells from H2O2-induced damage. Methods.


METHODS
epithelial AGS cells by measuring wound healing, cell proliferation, and cell viability. Global gene expression profiling was obtained by high-density microarray.


RESULTS
Hydrogen peroxide significantly delayed epithelial migration in wounded area. In contrast, eupatilin prevented the reduction of epithelial migration induced by H2O2. Eupatilin also ameliorated H2O2-induced actin disruption in AGS cells. Interestingly, treatment with eupatilin dramatically inhibited FeSO4-induced ROS production in a dose-dependent manner. In addition, eupatilin protected cells from FeSO4-induced F-actin disruption. With high-density microarray, we identified dozens of genes whose expressions were up-regulated in H2O2-treated cells. We found that eupatilin reduces the expression of such oxidative-responsible genes as HO-1, PLAUR and TNFRSF10A in H2O2-treated cells.


CONCLUSIONS
These results suggest that eupatilin acts as a novel antioxidant and may play an important role in DA-9601-mediated effective repair of the gastric mucosa.
Choi
Eun-Ju
EJ
Department of Life Science, GIST, Gwangju, 500-712, Korea.
Oh
Hyun-Mee
HM
Na
Bo-Ra
BR
Ramesh
T P
TP
Lee
Hyun-Ju
HJ
Choi
Chang-Soo
CS
Choi
Suck-Chei
SC
Oh
Tae-Young
TY
Choi
Suck-Jun
SJ
Chae
Jeong-Ryong
JR
Kim
Sang-Wook
SW
Jun
Chang-Duk
CD
D000199:Actins / Q000737:chemistry; D019610:Cytoprotection; D015536:Down-Regulation; D005419:Flavonoids / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
D000199:Actins; D005419:Flavonoids; D017382:Reactive Oxygen Species; C045325:eupatilin; D006861:Hydrogen Peroxide; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1