title: ATP production in isolated mitochondria of procyclic Trypanosoma brucei.
abstract: This chapter describes a luciferase-based protocol to measure adenosine triphosphate (ATP) production in isolated mitochondria of Trypanosoma brucei. The assay represents an excellent method to characterize the functionality of isolated mitochondria. Comparing the ATP production induced by substrates for oxidative phosphorylation to the one induced by substrates for substrate-level phosphorylation allows conclusions regarding the integrity of the outer and inner mitochondrial membranes. Furthermore, the assay is a valuable tool for characterization of RNA interference cell lines suspected to affect mitochondrial functions.
authors.affiliation: Department of Biology, University of Fribourg, Switzerland.
authors.forename: André
authors.initials: A
authors.lastname: Schneider
authors.forename: Nabile
authors.initials: N
authors.lastname: Bouzaidi-Tiali
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Chanez
authors.forename: Laurence
authors.initials: L
authors.lastname: Bulliard
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D004072:Digitonin / Q000494:pharmacology; D008156:Luciferases / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D013379:Substrate Specificity / Q000187:drug effects; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D008156:Luciferases; D004072:Digitonin