Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni.
A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n=23) and chronic (n=30) schistosomiasis, with other parasitoses (n=39) or without parasitic infections (n=100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in kappa concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P=0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.
de Oliveira
Edward José
EJ
Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Cj das Químicas, Bloco 17, 05508-900, São Paulo, SP, Brazil. edwardjo@cpqrr.fiocruz.br
Kanamura
Hermínia Yohko
HY
Takei
Kioko
K
Hirata
Rosario Dominguez Crespo
RD
Valli
Luiz Carlos Pedrosa
LC
Nguyen
Nga Yen
NY
de Carvalho Rodrigues
Izabel
I
de Jesus
Amélia Ribeiro
AR
Hirata
Mário Hiroyuki
MH
D000208:Acute Disease; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010446:Peptide Fragments / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments