Comparative effects of natural and synthetic diallyl disulfide on apoptosis of human breast-cancer MCF-7 cells.
The apoptotic effects of natural (n-) and synthetic (s-) DADS (diallyl disulfide; 3,3'-thiobisprop-1-ene) on human breast-cancer MCF-7 cells were investigated in vitro. 5-Fu (5-fluorouracil) and CTX (cyclophosphamide; Cytoxan) were used as comparative control anticancer agents. After MCF-7 cells had been treated with the drugs, cell viability, morphological change, apoptosis and changes in the cell cycle were measured. The results indicated that s- and n-DADS had similar cytotoxicities towards human breast-cancer MCF-7 cells and showed dose-dependent and time-dependent inhibitory effects. Morphological observations and flow-cytometric results showed that the four drugs (s-DADS, n-DADS, CTX and 5-Fu) induced apoptosis in MCF-7 cells to different extents. The apoptotic effects of s- and n-DADS were superior to those of 5-Fu and CTX.
Jun
Zou
Z
Research Institute for Molecular Pharmacology and Therapeutics, Central South University, Changsha, Hunan 410083, People's Republic of China.
Suzuki
Makiko
M
Xiao
Jianbo
J
Wen
Jun
J
Talbot
Simon G
SG
Li
Gloria C
GC
Xu
Ming
M
D000498:Allyl Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001688:Biological Products / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004220:Disulfides / Q000138:chemical synthesis* / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans
D000498:Allyl Compounds; D000970:Antineoplastic Agents; D001688:Biological Products; D004220:Disulfides; C028009:diallyl disulfide