The effects of long-duration, low-temperature ground transportation on physiological and biochemical indicators of stress in mice.
Transportation can cause stress to laboratory animals and alter physiological characteristics that may confound experimental results. The authors investigated stress-related effects of 3-4 h of transportation by truck in two strains of mice (C57BL/6, which are known to be aggressive, and ICR, which are less aggressive). Transported mice had sufficient space and access to water, though temperature in the truck was lower than what is usually recommended. Transportation affected the following parameters in both strains of mice: (i) serum corticosterone concentrations, (ii) expression of the chaperone proteins Hsp70 and Grp78 in various tissues and (iii) concentrations of serological enzymes that are associated with liver disease. These parameters also differed substantially between the two strains of mice.
Shim
Sun Bo
SB
Laboratory Animal Resources Team, National Institute of Toxicological Research, Korea FDA, Seoul 122-704, Korea.
Lee
Se Hyun
SH
Kim
Chuel Kyu
CK
Kim
Byoung Guk
BG
Kim
Yong Kyu
YK
Jee
Seung Wan
SW
Lee
Su Hae
SH
Sin
Ji Soon
JS
Bae
Chang Joon
CJ
Lee
Byoung Chun
BC
Jang
Mee Kyung
MK
Cho
Jung Sik
JS
Chae
Kab Ryong
KR
Hwang
Dae Youn
DY
D000822:Animal Husbandry; D000818:Animals; D001522:Behavior, Animal; D003080:Cold Temperature / Q000009:adverse effects*; D003345:Corticosterone / Q000097:blood; D000091342:Endoplasmic Reticulum Chaperone BiP; D004777:Environment; D004798:Enzymes / Q000097:blood; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018832:Molecular Chaperones / Q000378:metabolism; D013315:Stress, Psychological / Q000097:blood* / Q000503:physiopathology; D013997:Time Factors; D014186:Transportation
D000091342:Endoplasmic Reticulum Chaperone BiP; D004798:Enzymes; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D018832:Molecular Chaperones; D003345:Corticosterone