Effects of strontium fructose 1,6-diphosphate on expression of apoptosis-related genes and oxidative stress in testes of diabetic rats.
OBJECTIVE
To investigate the effects of strontium fructose 1,6-diphosphate (FDP-Sr) on testicular dysfunction induced by diabetes.


METHODS
Diabetes was induced by a single injection of streptozotocin (65 mg/kg, i.p.). After 28 days, therapy with three doses (50, 100, and 200 mg/kg per day, p.o.) of FDP-Sr was carried out for another 4 weeks.


RESULTS
The rats exhibited morphological lesions of testes and significant decreases in serum testosterone levels after 2 months of diabetes. Testicular tissues of diabetic rats showed significantly increased malondialdehyde levels and declined glutathione peroxidase activity. Meanwhile, augmented DNA fragmentation was observed, along with downregulated Bcl-2 and upregulated Bax expressions at both mRNA and protein levels. FDP-Sr showed significant antioxidant effects in both in vitro and in vivo experiments, and significantly relieved apoptosis and the decline of serum testosterone caused by diabetes.


CONCLUSIONS
Testicular injury and apoptosis induced by diabetes are partially attributed to the augmented oxidative stress in testicular tissue. FDP-Sr indirectly alleviates these pathologic alterations by suppressing the generation of reactive oxygen species.
Tang
Xiao-Yun
XY
Research Division of Pharmacology, China Pharmaceutical University, Nanjing, China.
Zhang
Qi
Q
Dai
De-Zai
DZ
Ying
Han-Jie
HJ
Wang
Qiu-Juan
QJ
Dai
Yin
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology*; D006597:Fructose-Bisphosphatase / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013324:Strontium / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects*
D006597:Fructose-Bisphosphatase; D013324:Strontium