Duplicate ditag analysis in LongSAGE.
The Long serial analysis of gene expression (SAGE) protocol generates ditags from tags with overlapping overhangs, thereby increasing the probability of duplicate ditag formation in LongSAGE. In this chapter, a tool is presented that facilitates the analysis of duplicate ditags in LongSAGE studies to determine whether they should be included or not.
Emmersen
Jeppe
J
Department of Biochemistry, Chemistry and Environmental Engineering, University of Aalborg, Aalborg, Denmark.
D001483:Base Sequence; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D017422:Sequence Analysis, DNA / Q000706:statistics & numerical data; D012984:Software