title: Detection of Anaplasma dna in Ixodes ricinus ticks: pitfalls.
abstract: A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to "Candidatus Ehrlichia walkefii". The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks.
authors.affiliation: Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Kvĕtná 8, 603 65 Brno, Czech Republic.
authors.forename: Silvie
authors.initials: S
authors.lastname: Sikutová
authors.forename: Ivo
authors.initials: I
authors.lastname: Rudolf
authors.forename: Maryna
authors.initials: M
authors.lastname: Golovchenko
authors.forename: Nataliia
authors.initials: N
authors.lastname: Rudenko
authors.forename: Libor
authors.initials: L
authors.lastname: Grubhoffer
authors.forename: Zdenĕk
authors.initials: Z
authors.lastname: Hubálek
mesh_terms: D000709:Anaplasma / Q000235:genetics*; D000818:Animals; D001179:Arthropod Vectors / Q000382:microbiology*; D001483:Base Sequence; D018153:Czech Republic / Q000453:epidemiology; D017931:DNA Primers; D018884:Ixodes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009758:Nymph / Q000382:microbiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D017931:DNA Primers