The identification and distribution of gonadotropin-releasing hormone-like peptides in the central nervous system and ovary of the giant freshwater prawn, Macrobrachium rosenbergii.
In the present study, we demonstrated the existence of GnRH-like peptides in the central nervous system (CNS) and ovary of the giant freshwater prawn, Macrobrachium rosenbergii using immunocytochemistry. The immunoreactivity (ir) of lamprey (l) GnRH-III was detected in the soma of medium-sized neurons located in neuronal cluster number 11 in the middle part of supraesophageal ganglion (deutocerebrum), whereas ir-octopus (oct) GnRH was observed in the soma of both medium-sized and large-sized neurons in thoracic ganglia, as well as in the fibers innervating the other medium-sized and large-sized neuronal cell bodies in the thoracic ganglia. In addition, ir-lGnRH-I was observed in the cytoplasm of late previtellogenic oocyte and early vitellogenic oocyte. These data suggest that M. rosenbergii contain at least three isoforms of GnRH: two GnRH isoforms closely related to lGnRH-III and octGnRH in the CNS, whereas another isoform, closely related to lGnRH-I, was localized in the ovary. This finding provides supporting data that ir-GnRH-like peptide(s) may exist in this decapod crustacean.
Ngernsoungnern
Apichart
A
Department of Anatomy, Faculty of Science, Mahidol University, Rama 6 Road, Rajathevi, Bangkok 10400, Thailand.
Ngernsoungnern
Piyada
P
Kavanaugh
Scott
S
Sower
Stacia A
SA
Sobhon
Prasert
P
Sretarugsa
Prapee
P
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D033364:Decapoda / Q000033:anatomy & histology* / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000145:classification / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*
D010455:Peptides; D007987:Gonadotropin-Releasing Hormone