Identification of novel genes and pathways affecting Salmonella type III secretion system 1 using a contact-dependent hemolysis assay.
We screened 5,700 Salmonella enterica serovar Typhimurium mutants for defects in type III secretion system 1 (T3SS-1)-mediated contact-dependent hemolysis to identify novel genes and pathways affecting the activity of T3SS-1. Our data suggest that previously unrecognized factors such as type I fimbriae may modulate the expression, activity, or deployment of this key virulence factor.
Field
Terry R
TR
Division of Microbiology, Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom.
Layton
Abigail N
AN
Bispham
Jennie
J
Stevens
Mark P
MP
Galyov
Edouard E
EE
D001426:Bacterial Proteins / Q000235:genetics; D001681:Biological Assay; D004268:DNA-Binding Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006461:Hemolysis; D009154:Mutation; D021381:Protein Transport / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics* / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C419207:FimW protein, Salmonella enterica; C419942:Spi1 protein, Salmonella; D037521:Virulence Factors