The Ezrin-radixin-moesin family member ezrin regulates stable microtubule formation and retroviral infection.
We recently identified the cytoskeletal regulatory protein moesin as a novel gene that inhibits retroviral replication prior to reverse transcription by downregulation of stable microtubule formation. Here, we provide evidence that overexpression of ezrin, another closely related ezrin-radixin-moesin (ERM) family member, also blocks replication of both murine leukemia viruses and human immunodeficiency virus type 1 (HIV-1) in Rat2 fibroblasts before reverse transcription, while knockdown of endogenous ezrin increases the susceptibility of human cells to HIV-1 infection. Together, these results suggest that ERM proteins may be important determinants of retrovirus susceptibility through negative regulation of stable microtubule networks.
Haedicke
Juliane
J
Centre for Research in Infectious Diseases, University College Dublin, Belfield, Dublin 4, Ireland.
de Los Santos
Kenia
K
Goff
Stephen P
SP
Naghavi
Mojgan H
MH
D000818:Animals; D003598:Cytoskeletal Proteins / Q000008:administration & dosage* / Q000502:physiology*; D004198:Disease Susceptibility / Q000628:therapy; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D009052:Leukemia Virus, Murine / Q000187:drug effects; D008565:Membrane Proteins; D051379:Mice; D008840:Microfilament Proteins; D008870:Microtubules / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000494:pharmacology; D051381:Rats; D012192:Retroviridae Infections / Q000188:drug therapy*; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D034741:RNA, Small Interfering; D000099149:Ezrin; C071058:moesin; C071776:radixin