title: Characterization of knockdown resistance in DDT- and pyrethroid-resistant Culex quinquefasciatus populations from Sri Lanka.
abstract: DDT and pyrethroid resistance in Culex quinquefasciatus have been previously reported in Sri Lanka, but the mechanisms involved have yet to be characterized. We report the presence of two mutant alleles of the sodium channel gene, the target site for both DDT and pyrethroid insecticides. Both mutations resulted in classic knockdown resistance (kdr) L1014F mutation because of either an A-to-T substitution or an A-to-C substitution. We developed two alternative assays to distinguish between the two mutations and used these to screen 214 individuals from nine geographic locations throughout Sri Lanka. Very high levels of kdr mutations were found throughout the country. A predominance of the A-to-C mutation was observed over the A-to-T with an average allele frequency of 50% and 2%, respectively. In addition to these non-synonymous kdr substitutions, we also found an indel (TCACA) in the intron downstream of the kdr mutation. After genotyping this indel in 136 individuals, we found no evident correlation between kdr genotypes and intronic indel. The presence of two alternative kdr mutations has implications for the reliance on single molecular diagnostics for detection of resistance in field populations. Furthermore, the high levels of these kdr mutations in C. quinquefasciatus populations throughout Sri Lanka are of concern for the future of pyrethroid-based control programmes on this island.
authors.affiliation: Liverpool School of Tropical Medicine, Liverpool, UK. c.s.wondji@liverpool.ac.uk
authors.forename: Charles S
authors.initials: CS
authors.lastname: Wondji
authors.forename: W A P
authors.initials: WA
authors.lastname: Priyanka De Silva
authors.forename: Janet
authors.initials: J
authors.lastname: Hemingway
authors.forename: Hilary
authors.initials: H
authors.lastname: Ranson
authors.forename: S H P
authors.initials: SH
authors.lastname: Parakrama Karunaratne
mesh_terms: D000483:Alleles; D000818:Animals; D003465:Culex / Q000187:drug effects / Q000235:genetics*; D003634:DDT / Q000494:pharmacology; D007303:Insect Vectors / Q000235:genetics*; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000494:pharmacology; D009154:Mutation; D011722:Pyrethrins / Q000494:pharmacology; D015222:Sodium Channels / Q000235:genetics*; D013188:Sri Lanka
chemical_list: D007306:Insecticides; D011722:Pyrethrins; D015222:Sodium Channels; D003634:DDT