title: Sertoli cell proliferation in the fetal and neonatal rat testis: a continuous phenomenon?
abstract: Sertoli cell population kinetics, as evidenced by semi-quantitative immunolabeling for proliferating cell nuclear antigen (PCNA) and Ki-67, in developing Wistar rat male gonads of embryos and neonates [14.5 days post conception (dpc)-7 days post partum (dpp)], was investigated. Throughout the examined period a gradual increase of immunolabeled Sertoli cell number, associated with intense mitotic activity, was observed. PCNA labeling index of Sertoli cells increased from 66.67 (at 14.5 dpc) to 89.74 (at 18.5 dpc) and then dropped to 75.24 (at 20.5 dpc). At birth, the percentage of PCNA immunoreactive Sertoli cells reached 98.70% and remained high thereafter, attaining a peak value of 99.90% at 7 dpp. The percentage of Ki-67 immunoreactive Sertoli cells in the fetal testis increased from E14.5 (43.95%) to E20.5 (77.40%). The proliferation rate did not alter considerably in the neonatal testis until 5 dpp. At this point, a significant increase of the Ki-67 labeling index was observed and a peak value of 95.76% was reached at 7 dpp. The pattern of Sertoli cell proliferation with age and the establishment of the final Sertoli cell number in vivo established in the present study was compared to the results from earlier investigations reported in the literature and the observed fluctuation of dividing cell numbers, associated with immunolabeling results throughout the examined period, complements and extends existing data. An appraisal of the timing of Sertoli cell proliferation in other species, namely mouse and man, is presented. The current investigation may be useful in evaluating the potential influence of factors interfering with normal mitotic activity of Sertoli cells, including cell selection mechanisms, such as apoptosis, senescence, DNA repair and hormonal/paracrine growth modulation.
authors.affiliation: Department of Histology and Embryology, Medical School, University of Athens, 75 Mikras Asias Street, Goudi, 115 27 Athens, Greece. rangelop@med.uoa.gr
authors.forename: R
authors.initials: R
authors.lastname: Angelopoulou
authors.forename: M
authors.initials: M
authors.lastname: Balla
authors.forename: G
authors.initials: G
authors.lastname: Lavranos
authors.forename: M
authors.initials: M
authors.lastname: Chalikias
authors.forename: C
authors.initials: C
authors.lastname: Kitsos
authors.forename: S
authors.initials: S
authors.lastname: Baka
authors.forename: C
authors.initials: C
authors.lastname: Kittas
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D005260:Female; D005333:Fetus / Q000166:cytology*; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism; D013737:Testis / Q000166:cytology* / Q000196:embryology / Q000254:growth & development
chemical_list: D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen