Principles of peripheral blood mononuclear cell apheresis in a preclinical canine model of hematopoietic cell transplantation.
BACKGROUND
Preclinical studies of peripheral blood mononuclear cell (PBMC) transplantation conducted in a well-established canine hematopoietic cell transplantation (HCT) model have been successfully translated to human patients over the past 5 decades.


OBJECTIVE
We retrospectively investigated the safety and feasibility of PBMC apheresis in the canine model of HCT by analyzing apheresis parameters, cell yields, and the impacts of donor-related and apheresis-related variables on collection yields and donor stability.


METHODS
One hundred and twenty dogs that underwent PBMC aphereses were evaluated.


METHODS
Aphereses were performed with a COBE Spectra blood separator and a central dual-lumen catheter, with or without recombinant canine granulocyte colony-stimulating factor (rcG-CSF) stem cell mobilization.


RESULTS
Aphereses from dogs not given rcG-CSF yielded an average volume of 280 +/- 42 mL containing an average of 15,086 +/- 9,834 leukocytes/mL. Aphereses from dogs given rcG-CSF yielded an average volume of 261 +/- 55 mL containing an average of 39,711 +/- 24,488 leukocytes/mL. Higher pre-apheresis white blood cell (WBC) counts correlated with higher apheresis WBC yields (R=0.50, P<.0001). The correlations of collection time, inlet volume, and collection flow rate on WBC yields were statistically significant but only weak to moderate in magnitude (R=0.34, P=.0001; R=0.38, P=.0006; R=0.26, P=.002, respectively) as were the correlations of collection time and inlet volume on collection volumes (R=0.30, P=.002; R=0.42, P<.0001, respectively). All dogs recovered promptly after PBMC aphereses and catheter removal, without complications.


CONCLUSIONS
These data may be useful for translating PBMC apheresis technology to the field of veterinary oncology for the treatment of dogs with hematologic malignancies.
Lupu
M
M
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. marilena.lupu@oregonstate.edu
Gooley
T
T
Zellmer
E
E
Graves
S S
SS
Storb
R
R
D017677:Age Distribution; D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D001835:Body Weight / Q000502:physiology; D004285:Dogs / Q000097:blood / Q000601:surgery*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation / Q000662:veterinary*; D007937:Leukapheresis / Q000379:methods / Q000662:veterinary*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008297:Male; D012189:Retrospective Studies; D012727:Sex Characteristics
D016179:Granulocyte Colony-Stimulating Factor