[Effect of Bulbus Allii Macrostemi on gene expression profile associated with vascular endothelium injure of Qi stagnation rats].
OBJECTIVE
To investigate the effect of Bulbus Allii Macrostemi on gene expression profile associated with inflammation and oxidative stress in vascular endothelium injure of qi stagnation rats.


METHODS
The model of vascular endothelium injure of qi stagnation rats were established by using high L-Methionine and being fastened. RT-PCR and serial analysis of gene expression (SAGE) database, available in NCBI, were used to analyze the changes of genes expression related to inflammation and oxidative stress in endothelium injure and the effect of Bulbus Allii Macrostemi.


RESULTS
Compared with model group, the genes expression of inflammation related COX-1 COX-2, oxidative stress related iNOS and eNOS, ECE involving in vascular vasomotion decreased (P < 0.05 or P < 0.01), but the gene expression of antioxidative SOD increased (P < 0.01) in Bulbus Allii Macrostemi group.


CONCLUSIONS
Bulbus Allii Macrostemi can adjust the disorder of the genes expression, related to vascular endothelium injure in Qi stagnation rats, protecting the vascular ehndothelium from injure.
Wu
Yi-ling
YL
Yiling Medical Research Institution of Hebei, Shijiazhuang 050035, China.
Liu
Kun
K
Qi
Jin-sheng
JS
Jia
Zhen-hua
ZH
Li
Yan-ning
YN
D000490:Allium / Q000737:chemistry*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D019413:Qi; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics
D004365:Drugs, Chinese Herbal; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases; D013482:Superoxide Dismutase