Isolation, gene detection and solvent tolerance of benzene, toluene and xylene degrading bacteria from nearshore surface water and Pacific Ocean sediment.
BTX (benzene, toluene and xylene) degrading bacteria were isolated from Pacific Ocean sediment and nearshore surface water. In the seawater near a ferry dock, degrading bacteria of a relatively wide diversity were detected, including species of Pseudomonas, Rhodococcus, Exiguobacterium and Bacillus; while species of Bacillus only have been detected from the deep-sea sediment. Most of the isolates showed degradation to more than one compound. Generally better growth was obtained with p-xylene and ethylbenzene than with the other two. All the bacteria could tolerate and grow with the compounds at 5-20% (v/v). Both benzene and toluene degradation related genes had been successfully PCR cloned from the isolates of nearshore water, the detected benzene dioxygenase gene was identical among all the species and close to its soil counterpart. However, they were not detected in all the isolates from deep sea. Results in this report suggested that BTX degrading bacteria widely spread in marine environments and they might be of potentials in biotreatment of BTEX in saline environments.
Wang
Lin
L
Key Laboratory of Marine Biogenetic Resources, The Third Institute of Oceanography, State Oceanic Administration, Daxue Road 178, Xiamen 361005, Fujian, People's Republic of China.
Qiao
Nan
N
Sun
Fengqin
F
Shao
Zongze
Z
D000222:Adaptation, Physiological; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001554:Benzene / Q000378:metabolism* / Q000633:toxicity; D001555:Benzene Derivatives / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D012997:Solvents / Q000378:metabolism* / Q000633:toxicity; D013624:Taiwan; D013696:Temperature; D014050:Toluene / Q000378:metabolism* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000378:metabolism* / Q000633:toxicity; D014992:Xylenes / Q000378:metabolism* / Q000633:toxicity
D001426:Bacterial Proteins; D001555:Benzene Derivatives; D012997:Solvents; D014874:Water Pollutants, Chemical; D014992:Xylenes; D014050:Toluene; D010105:Oxygenases; D001554:Benzene; C004912:ethylbenzene