title: Immunoglobulin mRNA and protein expression in human oral epithelial tumor cells.
abstract: In our previous work, we have reported the expression of immunoglobulin (Ig) molecules by numerous epithelial cancer cells and hyperplastic epithelial cells. In the present study, we extended our investigation to study the frequencies of expression of IgG and IgA in some types of oral epithelial tumor cells, and analyzed the oral tumor-derived V regions characteristic of Ig gamma chain gene transcripts by immunohistochemistry, in situ hybridization, laser capture microdissection-correlated reverse-transcription polymerase chain reaction, and sequencing. IgG and IgA immunoreactivity was prominent in the cytoplasmic or plasma membrane or secretion of malignant cells, pleomorphic adenoma tumor cells, and some normal glandular epithelial cells or squamous cells adjacent to tumors. More importantly, rearranged Ig gene transcripts were identified in these tumor cells, and in some normal glandular epithelial cells, the V-D-J region sequences revealed that IgG transcripts in 2 tested oral tumors were oligoclonal. These results support that the phenomenon of Ig could also be expressed in oral cavity epithelial tumor cells.
authors.affiliation: Peking University Center for Human Disease Genomics, Beijing, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhu
authors.forename: Cuiying
authors.initials: C
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Mao
authors.forename: Guohui
authors.initials: G
authors.lastname: Li
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Youhui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Qiu
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics / Q000276:immunology / Q000473:pathology; D001706:Biopsy; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D009062:Mouth Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology; D017421:Sequence Analysis; D050441:VDJ Exons / Q000235:genetics
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012333:RNA, Messenger