[Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC].
OBJECTIVE
To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae.


METHODS
The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection.


RESULTS
The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively.


CONCLUSIONS
The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions.
Zhang
Xiao-Guang
XG
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhi-Min
ZM
Song
Xiao-Mei
XM
Zhu
Jing-Jing
JJ
Gao
Hui-Min
HM
Lin
Li-Mei
LM
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D031239:Lonicera / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*
D012503:Saponins; C533461:loniceroside A; C533462:loniceroside C