title: IL-4/Stat6 activities correlate with apoptosis and metastasis in colon cancer cells.
abstract: IL-4-induced Stat6 signaling is active in a variety of cell types and plays a role in cell proliferation/growth and resistance to apoptosis. Using EMSA, we identified differential IL-4/Stat6 activities in colorectal cancer cell lines, HT-29 being active Stat6(high) phenotype and Caco-2 being defective Stat6(null) phenotype, respectively. Active Stat6(high) HT-29 cells exhibited resistance to apoptosis by flowcytometry and aggressive metastasis by Transwell assay compared with defective Stat6(null) Caco-2 cells. Comparing one another using RT-PCR, Stat6(high) HT-29 cells expressed more mRNA of anti-apoptotic and pro-metastatic genes Survivin, MDM2, and TMPRSS4, while Stat6(null) Caco-2 cells expressed more mRNA of pro-apoptotic and anti-metastatic genes BAX, CAV1, and P53, respectively. This is the first study describing correlations of IL-4/Stat6 activities with apoptosis and metastasis in colon cancer. These findings, together with the observation of constitutive Stat6 activation in many human malignancies, suggest that Stat6 activities could be a biomarker for cancer cell's invasive/metastatic capability.
authors.affiliation: Department of Medical Oncology, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan, Hubei 430071, China.
authors.forename: Ben Hui
authors.initials: BH
authors.lastname: Li
authors.forename: Xian Zi
authors.initials: XZ
authors.lastname: Yang
authors.forename: Pin Dong
authors.initials: PD
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Yuan
authors.forename: Xiao Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Yuan
authors.forename: Wen Jie
authors.initials: WJ
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000556:secondary*; D019073:HT29 Cells; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D050800:STAT6 Transcription Factor / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: C508598:IL4 protein, human; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; D015847:Interleukin-4