title: Differential anti-Golgi complex autoantibody production following murine lactate dehydrogenase-elevating virus infection.
abstract: Lactate dehydrogenase-elevating virus (LDV) causes asymptomatic infection and persistent viremia in mice with unique infectious specificity directed to a certain subpopulation of macrophages leading to chronic infection and an immunological disorder that includes hyperimmunoglobulinemia and production of autoantibodies. Infection with a species of LDV originally isolated from mice carrying an LDV-contaminated transplantable tumor (LDV-W) was reported to induce anti-Golgi complex antibody (AGA) production. In contrast, infection with the most common LDV species (LDV-P) was not associated with AGA production. Here we performed the first independent side by side comparison of the effects of the two LDV strains on their hosts as an initial approach to investigating the production of AGA. After viral inoculation, both LDV-W and LDV-P infected mice exhibited similar changes in lactate dehydrogenase in plasma suggesting similar viral activity. However, AGA production was observed in only the LDV-W infected mice and these mice exhibited plasma IgG elevation and immune complex formation. These data validated the differential potential of LDV-W and LDV-P in the production of AGA. Future comparative characterizations in the immune processing of Golgi complex autoantigens using these viral strains may be useful in obtaining specific insights in the specific anti-Golgi complex autoimmune responses.
authors.affiliation: Institute for Environment and Gender Specific Medicine, Juntendo University Graduate School of Medicine, Chiba, Japan. kazunozawa@juntendo-urayasu.jp
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nozawa
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Fritzler
authors.forename: Keigo
authors.initials: K
authors.lastname: Ikeda
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Takasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Edward K L
authors.initials: EK
authors.lastname: Chan
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex / Q000096:biosynthesis; D018174:Arterivirus Infections / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis*; D015551:Autoimmunity / Q000276:immunology*; D006056:Golgi Apparatus / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007772:Lactate dehydrogenase-elevating virus / Q000302:isolation & purification / Q000648:ultrastructure; D051379:Mice; D008815:Mice, Inbred Strains; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007074:Immunoglobulin G; D007770:L-Lactate Dehydrogenase