title: HaploSNPer: a web-based allele and SNP detection tool.
abstract: BACKGROUND
  Single nucleotide polymorphisms (SNPs) and small insertions or deletions (indels) are the most common type of polymorphisms and are frequently used for molecular marker development. Such markers have become very popular for all kinds of genetic analysis, including haplotype reconstruction. Haplotypes can be reconstructed for whole chromosomes but also for specific genes, based on the SNPs present. Haplotypes in the latter context represent the different alleles of a gene. The computational approach to SNP mining is becoming increasingly popular because of the continuously increasing number of sequences deposited in databases, which allows a more accurate identification of SNPs. Several software packages have been developed for SNP mining from databases. From these, QualitySNP is the only tool that combines SNP detection with the reconstruction of alleles, which results in a lower number of false positive SNPs and also works much faster than other programs. We have build a web-based SNP discovery and allele detection tool (HaploSNPer) based on QualitySNP.
  
  
  RESULTS
  HaploSNPer is a flexible web-based tool for detecting SNPs and alleles in user-specified input sequences from both diploid and polyploid species. It includes BLAST for finding homologous sequences in public EST databases, CAP3 or PHRAP for aligning them, and QualitySNP for discovering reliable allelic sequences and SNPs. All possible and reliable alleles are detected by a mathematical algorithm using potential SNP information. Reliable SNPs are then identified based on the reconstructed alleles and on sequence redundancy.
  
  
  CONCLUSIONS
  Thorough testing of HaploSNPer (and the underlying QualitySNP algorithm) has shown that EST information alone is sufficient for the identification of alleles and that reliable SNPs can be found efficiently. Furthermore, HaploSNPer supplies a user friendly interface for visualization of SNP and alleles. HaploSNPer is available from http://www.bioinformatics.nl/tools/haplosnper/.
authors.affiliation: Laboratory of Bioinformatics, Wageningen University, PO Box 8128, 6700 ET Wageningen, The Netherlands. jack.leunissen@wur.nl.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Tang
authors.forename: Jack A M
authors.initials: JA
authors.lastname: Leunissen
authors.forename: Roeland E
authors.initials: RE
authors.lastname: Voorrips
authors.forename: C Gerard
authors.initials: CG
authors.lastname: van der Linden
authors.forename: Ben
authors.initials: B
authors.lastname: Vosman
mesh_terms: D000465:Algorithms; D000483:Alleles; D000818:Animals; D016000:Cluster Analysis; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D020407:Internet; D011003:Ploidies; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*