Release of immune modulation factors from platelet concentrates during storage after photochemical pathogen inactivation treatment.
BACKGROUND
Blood platelets (PLTs) are critical for hemostasis, and they contain biologically active constituents with the potential to modulate inflammatory responses. This study examined the effects of photochemical pathogen inactivation treatment (PCT) on the release of cytokines and/or chemokines from PLT components.


METHODS
Double-dose apheresis PLT components were suspended in plasma-PLT additive solution mixtures and divided into paired therapeutic units. One unit served as an untreated control and the other unit was treated with PCT. PLT concentrations, pH, and levels of cytokines and/or chemokines (CD62p, platelet-derived growth factor-AB, interleukin [IL]-8, soluble CD40 ligand [sCD40L], IL-1beta, and tumor necrosis factor alpha) were measured during 7 days of storage in PLT component supernatants and PLT lysates.


RESULTS
PLT content, pH, and cytokine and/or chemokine content and release from PLT component prepared with PCT were not different (p > 0.05) from paired control components during storage. Levels of sCD40L, however, increased significantly during storage while decreasing in parallel within PLT lysates, although no differences were detected between paired PCT and control PLT component.


CONCLUSIONS
PCT did not increase the release or secretion of PLT chemokines and/or cytokines over a 7-day period compared to conventional PLT component.
Cognasse
Fabrice
F
Blood Transfusion Center, EFS Auvergne-Loire, Saint-Etienne, France.
Osselaer
Jean-Claude
JC
Payrat
Jean Marc
JM
Chavarin
Patricia
P
Corash
Laurence
L
Garraud
Olivier
O
D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000276:immunology* / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods*; D017848:Blood-Borne Pathogens; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism*; D010777:Photochemistry; D017713:Platelet Transfusion
D015415:Biomarkers; D018925:Chemokines; D016207:Cytokines; D007155:Immunologic Factors