PU.1 and CEBPA expression in acute myeloid leukemia.
Alterations of the transcription factors CCAAT/enhancer binding protein alpha (CEBPA) and PU.1 have been described in acute myeloid leukemia (AML). We studied CEBPA and PU.1 mRNA levels by real-time RT-PCR in 109 primary AML samples, compared with normal bone marrow and peripheral blood cells. Low PU.1 levels were observed in monoblastic leukemias, while low CEBPA levels were associated with leukopenia at diagnosis and lack of expression of differentiation antigens CD33 and CD11c. We conclude that down-regulation of CEBPA and PU.1 is not a general feature of primary AML, but appears to be restricted to distinct AML subtypes.
D'Alò
Francesco
F
Institute of Hematology, Università Cattolica del Sacro Cuore, L.go A. Gemelli 8, Rome 00168, Italy.
Di Ruscio
Annalisa
A
Guidi
Francesco
F
Fabiani
Emiliano
E
Greco
Mariangela
M
Rumi
Carlo
C
Hohaus
Stefan
S
Voso
Maria Teresa
MT
Leone
Giuseppe
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D039521:CD11c Antigen / Q000235:genetics / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007970:Leukopenia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D063268:Sialic Acid Binding Ig-like Lectin 3; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D022762:CCAAT-Enhancer-Binding Proteins; D039521:CD11c Antigen; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D063268:Sialic Acid Binding Ig-like Lectin 3; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C571163:CD33 protein, human; C515848:CEBPA protein, human