[Determination of artemisinin in Artemisia annua by the ultraviolet spectrophotometric].
OBJECTIVE
To establish a simple method to determine artemisinin in Artemisia annua by ultraviolet spectrophotometric and determine the content of artemisinin in Artemisia annua from different places by this new method.


METHODS
Carrying on the wave length scanning with the ultraviolet spectrophotometer to arteannuin standard solution, establishing the standard curve, according to standard curve determination sample content.


RESULTS
The most suitable absorption wave length of artemisinin in Artemisia annua was 292 nm, and the extinction-density regression equation was: A = 0.05826C - 0.00117, r = 0.9999.


CONCLUSIONS
The method of ultraviolet spectrophotometric determining artemisinin in Artemisia annua is rapid and convenient. It can be used for the quality control of this herbal medicine.
D031172:Artemisia annua / Q000737:chemistry* / Q000254:growth & development; D037621:Artemisinins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000592:standards; D000431:Ethanol; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D037621:Artemisinins; D004365:Drugs, Chinese Herbal; D000431:Ethanol