Elucidation of 2-hydroxybiphenyl effect on dibenzothiophene desulfurization by Microbacterium sp. strain ZD-M2.
The effect of 2-hydroxybiphenyl (2-HBP), the end product of dibenzothiophene (DBT) desulfurization via 4S pathway, on cell growth and desulfurization activity was investigated by Microbacterium sp. The experimental results indicate that 2-HBP would inhibit the desulfurization activity. Providing 2-HBP was added in the reaction media, the DBT degradation rate decreased along with the increase of 2-HBP addition. By contrast, cell growth would be promoted in the addition of 2-HBP at a low concentration (<0.1mM). At high concentration of 2-HBP, the inhibition on the cell growth occurred. Meanwhile, the inhibitory effect of 2-HBP on DBT desulfurization activity was tested both in the oil/aqueous two-phase system and the aqueous system. A mathematical model was developed to explain the product formation kinetics with DBT as the sole sulfur source. The predicted results were close to the experimental data, it elucidated that along with the 2-HBP accumulation, the inhibitory effect of 2-HBP on DBT desulfurization and cell growth was enhanced.
Chen
Han
H
Institute of Environmental Engineering, Zhejiang University (Yuquan Campus), Hangzhou 310027, China.
Zhang
Wen-Juan
WJ
Cai
Yu-Bei
YB
Zhang
Ying
Y
Li
Wei
W
D001713:Biphenyl Compounds / Q000378:metabolism*; D003470:Culture Media; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D007700:Kinetics; D013455:Sulfur / Q000378:metabolism*; D013876:Thiophenes / Q000378:metabolism*
D001713:Biphenyl Compounds; D003470:Culture Media; D013876:Thiophenes; D013455:Sulfur; C004369:2-phenylphenol; C016366:dibenzothiophene