Heterologous protein secretion by Lactobacillus plantarum using homologous signal peptides.
OBJECTIVE
To test seven selected putative signal peptides from Lactobacillus plantarum WCFS1 in terms of their ability to drive secretion of two model proteins in Lact. plantarum, and to compare the functionality of these signal peptides with that of well-known heterologous signal peptides (Usp45, M6).


RESULTS
Signal peptide functionality was assessed using a series of modular derivatives of the pSIP vectors for peptide pheromone-controlled high-level gene expression in lactobacilli. Several of the constructs with homologous signal peptides yielded similar or higher reporter protein activities than constructs with heterologous signal peptides. Two of the homologous signal peptides (Lp_0373 and Lp_0600) appeared as especially promising candidates for directing secretion, as they were among the best performing with both reporter proteins.


CONCLUSIONS
We have identified homologous signal peptides for high-level secretion of heterologous proteins in Lact. plantarum. With the model proteins, some of these performed better than commonly used heterologous signal peptides.


CONCLUSIONS
The homologous signal peptides tested out, in this study, could be useful in food-grade systems for secretion of interesting proteins in Lact. plantarum. The constructed modular secretion vectors are easily accessible for rapid signal peptide screening.
Mathiesen
G
G
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, As, Norway. geir.mathiesen@umb.no
Sveen
A
A
Piard
J-C
JC
Axelsson
L
L
Eijsink
V G H
VG
D000681:Amylases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005516:Food Microbiology; D015870:Gene Expression; D017930:Genes, Reporter; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000494:pharmacology; D048191:Lactiplantibacillus plantarum / Q000378:metabolism / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000494:pharmacology; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D000681:Amylases