Pro-inflammatory cytokine production from normal human fibroblasts is induced by Tannerella forsythia detaching factor.
OBJECTIVE
Tannerella forsythia is a periodontal pathogen. Recently, we have reported that the cytopathic component of T. forsythia contains two distinct factors. One arrests the cell cycle at the G2 phase and the other, named forsythia detaching factor, detaches adhesion-dependent immortalized human cells. In this study, we investigated the biological function of forsythia detaching factor using human normal fibroblasts.


METHODS
A recombinant forsythia detaching factor, reported previously, was used. TIG-3 cells, cultured in the absence or presence of forsythia detaching factor, were lysed and the supernatant was analyzed by western blotting with polyclonal forsythia detaching factor antibodies. The cells were subsequently fractionated to isolate the cytoplasmic, mitochondrial and remaining fractions. In order to measure the activity of mitochondria using nicotinamide adenine dinucleotide-linked reductase, the water-soluble tetrazolium method was used. The mitochondrial oxidative membrane potential was estimated by measuring the oxidization-dependent fluorogenic conversion of dihydrotetramethylrosamine using flow cytometry. The concentration of interleukin-8 in the culture supernatant was assayed using a Human IL-8 ELISA kit.


RESULTS
Forsythia detaching factor-treated cells detached from the substratum and aggregated from 3 to 24 h. Then, the detached cells resumed adhesion and proliferated after 48 h. The western blot analysis revealed that most forsythia detaching factor trans-located into the mitochondrial fraction. Forsythia detaching factor suppressed the nicotinamide adenine dinucleotide-linked reductase activity in a dose-dependent manner and consequently increased the mitochondrial oxidative membrane potential. The production of interleukin-8 was reinforced in forsythia detaching factor-treated cells at 72 h through an increase of the mitochondrial oxidative membrane potential.


CONCLUSIONS
The forsythia detaching factor might be involved in the virulence of T. forsythia through induction of the pro-inflammatory cytokine interleukin-8.
Tomi
N
N
Department of Molecular Cellular Oncology and Microbiology, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
Fukuyo
Y
Y
Arakawa
S
S
Nakajima
T
T
D001426:Bacterial Proteins / Q000502:physiology*; D001439:Bacteroides / Q000737:chemistry* / Q000472:pathogenicity; D002448:Cell Adhesion / Q000187:drug effects; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors; D010084:Oxidation-Reduction; D010518:Periodontitis / Q000382:microbiology; D011994:Recombinant Proteins / Q000494:pharmacology; D037521:Virulence Factors / Q000502:physiology*
D001426:Bacterial Proteins; D002457:Cell Extracts; D016209:Interleukin-8; D011994:Recombinant Proteins; D037521:Virulence Factors; D009245:NADH Dehydrogenase