[Usefulness of PCR in rapidly diagnosing subcutaneous abscess and costal osteomyelitis caused by Mycobacterium bovis BCG].
Conventional polymerase chain reaction (PCR) used to identify mycobacterial species does not distinguish between Mycobacterium tuberculosis and M. bovis BCG, and several weeks or months may be needed to identify individual slow-growing Mycobacterial species. We report a 4-year-old girl who had subcutaneous abscess and sternal osteomyelitis after BCG vaccination at 4 month of age. We directly identified M. bovis BCG genome in the punctured abscess within a few days using PCR and PCR-restriction fragment length polymorphism. Such PCR is useful for rapidly diagnosing and managing of appropriate therapy in patients with infection due to M. bovis BCG.
Ishiwada
Naruhiko
N
Department of Pediatrics, Chiba University Graduate School of Medicine.
Hishiki
Haruka
H
Watanabe
Masaharu
M
Ohkusu
Kiyofumi
K
Kohno
Yoichi
Y
D000038:Abscess / Q000175:diagnosis*; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D009163:Mycobacterium bovis / Q000235:genetics; D010019:Osteomyelitis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012272:Ribs; D014376:Tuberculosis / Q000175:diagnosis*; D014394:Tuberculosis, Osteoarticular / Q000175:diagnosis*
D004269:DNA, Bacterial