Galanin immunoreactivity increased in chicken supraoptic neurons after activation of the vasotocin system at oviposition.
The avian arginine vasotocin (AVT) synthesized in the hypothalamic magnocellular neurons and released from the posterior pituitary is known to be involved in the regulation of uterine contractions for oviposition in chickens. However, regulation of AVT synthesis and release within the magnocellular hypothalamus has not been elucidated. Galanin, the oviposition inducing factor in the oviduct of the hen, has been demonstrated to have sexually dimorphic stimulatory action in oxytocin- and vasopressin neurons in the mammalian hypothalamus. In this study, galanin and AVT immunoreactivity was investigated around the time of oviposition in the supraoptic nucleus (SON) to determine if galanin modulates AVT synthesis and/or release. Within SON neurons increased AVT immunoreactivity before oviposition and the decreased AVT immunoreactivity after oviposition implied function-related peptide release. The significantly increased number of galanin neurons co-localizing with AVT immediately after oviposition suggests that galanin is involved in the negative feedback to limit AVT release in the SON. Thus, these data support the idea that AVT in the SON is involved in central regulation of oviposition and that AVT release could be modulated by the neuropeptide galanin.
Klein
Sabine
S
Department of Functional Genomics and Bioregulation, Institute for Farm Animal Genetics, Federal Institute for Animal Health (FLI), 31535 Neustadt, Höltystr. 10, Germany. sabine.klein@fli.bund.de
Grossmann
Roland
R
D000818:Animals; D002645:Chickens / Q000502:physiology*; D025461:Feedback, Physiological; D005260:Female; D019004:Galanin / Q000032:analysis; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000378:metabolism; D010058:Oviposition / Q000502:physiology*; D013495:Supraoptic Nucleus / Q000378:metabolism*; D014668:Vasotocin / Q000032:analysis / Q000378:metabolism*
D019004:Galanin; D014668:Vasotocin