title: A dual-purpose synthetic colloidal platform for protease mapping: substrate profiling for Dengue and West Nile virus proteases.
abstract: In a proof of concept study, we created a small focused fluorescent hexapeptide library onto 14 multiplexed barcoded sets of silica particles to probe the substrate recognition specificity of West Nile and Dengue virus proteases. A flow cytometric analysis demonstrated that the optical signature of each bead population remained distinguishable throughout the solid-phase peptide synthesis and proteolytic assay. As expected, both proteases displayed a narrow specificity for lysine and arginine residues in the P(1) and P(2) substrate positions. This open-ended platform enables the fast and simultaneous identification of peptide substrates and is applicable to other proteases.
authors.affiliation: Nanotechnology and Biomaterials Centre, Level 5 East, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Saint Lucia, QLD 4072, Australia. l.marcon@uq.edu.au
authors.forename: Lionel
authors.initials: L
authors.lastname: Marcon
authors.forename: Darby
authors.initials: D
authors.lastname: Kozak
authors.forename: Bronwyn J
authors.initials: BJ
authors.lastname: Battersby
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Chappell
authors.forename: David P
authors.initials: DP
authors.lastname: Fairlie
authors.forename: Paul
authors.initials: P
authors.lastname: Young
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D001120:Arginine / Q000378:metabolism; D003716:Dengue Virus / Q000201:enzymology*; D005434:Flow Cytometry; D008239:Lysine / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D019151:Peptide Library; D013379:Substrate Specificity; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism*; D014902:West Nile virus / Q000201:enzymology*
chemical_list: D019151:Peptide Library; D014764:Viral Proteins; D001120:Arginine; D010447:Peptide Hydrolases; D008239:Lysine