title: Identification of herbarium whole-leaf samples of Epilobium species by ATR-IR spectroscopy.
abstract: A simple, high-accuracy FT-IR method based on attenuated total reflection (ATR) was developed for the rapid determination of leaf samples of Epilobium species. The method is superior to other analytical techniques, since there is no need of laborious sample preparation such as grinding or extraction and solvent removal. A total of 70 herbarium specimens, belonging to all 13 Epilobium and to 2 Chamerion species growing in Slovenia, were analyzed. With the 100 most-informative wavenumbers in the range 700-1800 cm(-1), we obtained over 90% accuracy of species identification, with discriminant multivariate statistical analysis on the measurements made on whole dried leaves.
authors.affiliation: Department of Biology, Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia. simona.strgulc@bf.uni-lj.si
authors.forename: Simona
authors.initials: S
authors.lastname: Strgulc Krajsek
authors.forename: Primoz
authors.initials: P
authors.lastname: Buh
authors.forename: Anamarija
authors.initials: A
authors.lastname: Zega
authors.forename: Samo
authors.initials: S
authors.lastname: Kreft
mesh_terms: D016000:Cluster Analysis; D031666:Epilobium / Q000737:chemistry* / Q000145:classification*; D029594:Onagraceae / Q000737:chemistry / Q000145:classification; D018515:Plant Leaves / Q000737:chemistry*; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods