title: An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea.
abstract: The H9N2 subtype low pathogenic avian influenza is one of the most prevalent avian diseases worldwide, and was first documented in 1996 in Korea. This disease caused serious economic loss in Korea's poultry industry. In order to develop an oil-based inactivated vaccine, a virus that had been isolated in 2001 (A/chicken/Korea/01310/2001) was selected based on its pathogenic, antigenic, and genetic properties. However, in animal experiments, the efficacy of the vaccine was found to be very low without concentration of the antigen (2 7 to 2 10 hemagglutinin unit). In order to overcome the low productivity, we passaged the vaccine candidate virus to chicken eggs. After the 20th passage, the virus was approximately ten times more productive compared with the parent virus. For the most part, the passaged virus maintained the hemagglutinin cleavage site amino acid motif (PATSGR/GLF) and had only three amino acid changes (T133N, V216G, E439D, H3 numbering) in the hemagglutinin molecule, as well as 18 amino acid deletions (55-72) and one amino acid change (E54D) in the NA stalk region. The amino acid changes did not significantly affect the antigenicity of the vaccine virus when tested by hemagglutination inhibition assay. Though not complete, the vaccine produced after the 20th passage of the virus (01310 CE20) showed good protection against a homologous and recent Korean isolate (A/chicken/Korea/Q30/2004) in specific pathogen- free chickens. The vaccine developed in this study would be helpful for controlling the H9N2 LPAI in Korea.
authors.affiliation: National Veterinary Research and Quarantine Service, Anyang 430-824, Korea.
authors.forename: Jun Gu
authors.initials: JG
authors.lastname: Choi
authors.forename: Youn Jeong
authors.initials: YJ
authors.lastname: Lee
authors.forename: Yong Joo
authors.initials: YJ
authors.lastname: Kim
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Lee
authors.forename: Ok Mi
authors.initials: OM
authors.lastname: Jeong
authors.forename: Haan Woo
authors.initials: HW
authors.lastname: Sung
authors.forename: Jae Hong
authors.initials: JH
authors.lastname: Kim
authors.forename: Jun Hun
authors.initials: JH
authors.lastname: Kwon
mesh_terms: D000818:Animals; D002645:Chickens; D015967:Gene Expression Regulation, Viral; D006388:Hemagglutinins / Q000235:genetics; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology*; D005585:Influenza in Birds / Q000453:epidemiology / Q000517:prevention & control* / Q000821:virology*; D007723:Korea / Q000453:epidemiology; D009439:Neuraminidase / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000276:immunology*
chemical_list: D006388:Hemagglutinins; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D009439:Neuraminidase