Integrating sensitive quantification of hepatic metabolic functions by capillary electrophoresis with laser-induced fluorescence detection.
We report the establishment of capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection as a common analytical platform for sensitive quantification of both phase I and II metabolism in various hepatic in vitro models.
Toh
Yi-Chin
YC
Institute of Biotechnology and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore 138669, Singapore.
Zhang
Jing
J
Khong
Yuet Mei
YM
Du
Yanan
Y
Sun
Wanxin
W
Yu
Hanry
H
D000818:Animals; D018929:Cell Culture Techniques; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007834:Lasers; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes