title: Highly sensitive rapid, reliable, and automatic cardiovascular disease diagnosis with nanoparticle fluorescence enhancer and mems.
abstract: Cardiovascular diseases (CVDs) have been the leading threat to human life. An effective way for sensitive and accurate CVD diagnosis is to measure the biochemical markers released from the damaged myocardial cells in the bloodstream. Here, a multi-analyte, fluorophore mediated, fiber-optic immuno-biosensing system is being developed to simultaneously and rapidly quantify four clinically important cardiac markers, myoglobin, C-reactive protein, cardiac troponin I, and B-type natriuretic peptide. To quantify these markers at a pico-molar level, novel nanoparticle reagents enhancing fluorescence were used and signal enhancement was obtained as high as approximately 230%. Micro-electro-mechanical system (MEMS) was integrated to this system to ensure a reliable and fully-automated sensing performance. A point-of-care, automatic microfluidic sensing system for four cardiac marker quantification was developed with the properties of 3 cm sensor size, 300 microL sample volume, 9-minute assay time, and an average signal-to-noise ratio of 35.
authors.affiliation: Department of Chemical Engineering, University of Louisville, Louisville, KY 40292, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Hong
authors.forename: Junhai
authors.initials: J
authors.lastname: Kai
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Ren
authors.forename: Jungyoup
authors.initials: J
authors.lastname: Han
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zou
authors.forename: Chong H
authors.initials: CH
authors.lastname: Ahn
authors.forename: Kyung A
authors.initials: KA
authors.lastname: Kang
mesh_terms: D015415:Biomarkers / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D002318:Cardiovascular Diseases / Q000175:diagnosis*; D003935:Diagnostic Techniques, Cardiovascular / Q000295:instrumentation; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D053758:Nanoparticles; D010316:Particle Size; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes