Detection of GFP during nervous system development in Drosophila melanogaster.
Using the vital marker GFP and its spectral variants, it is possible to visualize multiple proteins in individual cells and thereby monitor embryonic development on a cellular and molecular level. In the following chapter we describe how to prepare Drosophila embryos or larvae for live imaging or immunohistochemical staining and provide some guidelines for optimal GFP detection.
Edoff
Karin
K
Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, USA.
Dods
James S
JS
Brand
Andrea H
AH
D000818:Animals; D004331:Drosophila melanogaster / Q000196:embryology* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005434:Flow Cytometry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics; D049408:Luminescent Agents / Q000032:analysis*; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism
D049408:Luminescent Agents; D049452:Green Fluorescent Proteins