Blood monocyte subsets differentially give rise to CD103+ and CD103- pulmonary dendritic cell populations.
There are two major myeloid pulmonary dendritic cell (DC) populations: CD103+ DCs and CD11bhigh DCs. In this study, we investigated in detail the origins of both myeloid DC pools using multiple experimental approaches. We show that, in resting lung, Ly-6ChighCCR2high monocytes repopulated CD103+ DCs using a CCR2-dependent mechanism, and these DCs preferentially retained residual CCR2 in the lung, whereas, conversely, Ly-6ClowCCR2low monocytes repopulated CD11bhigh DCs. CX3CR1 was required to generate normal numbers of pulmonary CD11bhigh DCs, possibly because Ly-6Clow monocytes in the circulation, which normally express high levels of CX3CR1, failed to express bcl-2 and may have diminished survival in the circulation in the absence of CX3CR1. Overall, these data demonstrate that the two circulating subsets of monocytes give rise to distinct tissue DC populations.
Jakubzick
Claudia
C
Department of Gene and Cell Medicine, Icahn Research Institute, Mount Sinai School of Medicine, New York, NY 10029, USA.
Tacke
Frank
F
Ginhoux
Florent
F
Wagers
Amy J
AJ
van Rooijen
Nico
N
Mack
Matthias
M
Merad
Miriam
M
Randolph
Gwendalyn J
GJ
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism; D000950:Antigens, Ly / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D039481:CD11b Antigen / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000145:classification / Q000276:immunology* / Q000378:metabolism*; D039001:Integrin alpha Chains / Q000096:biosynthesis* / Q000097:blood / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009000:Monocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D011828:Radiation Chimera / Q000235:genetics / Q000276:immunology; D054390:Receptors, CCR2 / Q000097:blood / Q000172:deficiency / Q000235:genetics
D015703:Antigens, CD; D000950:Antigens, Ly; D015415:Biomarkers; D039481:CD11b Antigen; C515335:Ccr2 protein, mouse; D039001:Integrin alpha Chains; C494192:Ly-6C antigen, mouse; D054390:Receptors, CCR2; C091367:alpha E integrins