CCR5-dependent regulatory T cell migration mediates fungal survival and severe immunosuppression.
Paracoccidioidomycosis, a debilitating pulmonary mycosis, is caused by the dimorphic fungus Paracoccidioides brasiliensis. The infection results in the formation of granulomas containing viable yeast cells that are the fungal sources for disease reactivation. Because CD4+CD25+ regulatory T cells (Tregs) are in the lesions of patients with paracoccidioidomycosis, the migration of Treg cells is dependent on the axis chemokine-chemokine receptors, and CCR5 ligands are produced in P. brasiliensis-induced lesions, we investigated the role of CCR5 in the control of the infection. The results showed that CCR5-/- mice are more efficient in controlling fungal growth and dissemination and exhibited smaller granulomas than wild-type (WT) mice. In the absence of CCR5, the percentage of CD4+CD25+ T cells expressing Foxp3, glucocorticoid-induced TNFR (GITR), CD103, CD45low, and CTLA-4 in the granulomas was significantly decreased. Interestingly, P. brasiliensis infection resulted in an absence of T cell proliferation in response to Con A in WT but not CCR5-/- mice that was abrogated by anti-CTLA-4 mAb and anti-GITR mAb. Moreover, the adoptive transfer of CD4+CD25+ but not CD4+CD25- T cells from infected WT to infected CCR5-/- mice resulted in a significant increase in fungal load. Overall, CCR5 is a key receptor for the migration of Treg cells to the site of P. brasiliensis infection, leading to down-modulation of effector immune response and the long-term presence of the fungus in the granulomas. Thus, a tight control of Treg cell migration to the granulomatous lesions could be an important mechanism for avoiding exacerbation and reactivation of the disease.
Moreira
Ana Paula
AP
Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, Universidade de São Paulo, Brazil.
Cavassani
Karen Angelica
KA
Massafera Tristão
Fabrine Sales
FS
Campanelli
Ana Paula
AP
Martinez
Roberto
R
Rossi
Marcos Antonio
MA
Silva
João S
JS
D000818:Animals; D002464:Cell Migration Inhibition / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D006099:Granuloma / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D007111:Immunity, Cellular / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010228:Paracoccidioides / Q000254:growth & development* / Q000276:immunology* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D019713:Receptors, CCR5 / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology
D019713:Receptors, CCR5