title: [Experimental study of sentinel lymph node biopsy in thyroid by using three kinds of vital dyes at different concentration and dose].
abstract: OBJECTIVE
  Using isosulfan blue (IB), patent blue (PB) and methylene blue (MB) at different concentration and dose in the thyroid of rabbits, to study the role of them in the sentinel lymph node (SLN) biopsy in rabbit and to provide experimental evidence for the choice of ideal dye of the sentinel lymph node biopsy in thyroid cancer patients.
  
  
  METHODS
  Thirty-six adult rabbits were randomly divided into nine groups with four rabbits in each group. After 1%-0.01 ml, 1%-0.02 ml, 2%-0.02 ml of IB, PB, MB were separately injected in the thyroid of rabbit, the number of SLNs and the dying time, and the beginning and complete fading time of the SLNs were observed.
  
  
  RESULTS
  One to 3 SLNs were identified in the one side neck of rabbits and there were no significant difference among different groups. The average time from the vital blue injection in the thyroid to SLN being stained was 6.3 s by using 2% MB 0.02 ml, which was the shortest. The lymph nodes stained by 2% MB 0.02 ml began to fade after 28.2 min, and the lasting time is the longest. The SLNs stained by using 1% MB 0.02 ml or 2% MB 0.02 ml can be identified after 40 mins.
  
  
  CONCLUSIONS
  The study confirmed that 2% MB 0.02 ml were better than the other vital dyes for sentinel lymph node biopsy. The lymph nodes were stained by 2% MB 0.02 ml deeply and the dying time is the shortest, while the lasting time is the longest.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Renji Hospital Jiaotong University, Shanghai, 200001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jiadong
authors.initials: J
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000818:Animals; D004396:Coloring Agents; D008198:Lymph Nodes / Q000473:pathology; D008751:Methylene Blue; D011817:Rabbits; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013961:Thyroid Gland / Q000473:pathology*
chemical_list: D004396:Coloring Agents; D012394:Rosaniline Dyes; C025484:iso-sulfan blue; D008751:Methylene Blue