title: Co-infection with two different strains of Bordetella pertussis in an infant.
abstract: We report co-infection with two phenotypically and genotypically distinct strains of Bordetella pertussis in an infant male hospitalized with a 2-week history of cough, paroxysms and vomiting. Colonies from the two B. pertussis phenotypes were isolated and evaluated by PFGE profile analysis, gene sequence typing and PCR-RFLP of a portion of the 23S rRNA gene. These results demonstrated simultaneous infection with two different strains of B. pertussis.
authors.affiliation: National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Pamela K
authors.initials: PK
authors.lastname: Cassiday
authors.affiliation: Georgia Department of Human Resources, Atlanta, GA, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Tobin-D'Angelo
authors.affiliation: Northlake Medical Center, Tucker, GA, USA.
authors.forename: J Renee
authors.initials: JR
authors.lastname: Watson
authors.affiliation: National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Kai-Hui
authors.initials: KH
authors.lastname: Wu
authors.affiliation: Georgia Department of Human Resources, Atlanta, GA, USA.
authors.forename: Mahin M
authors.initials: MM
authors.lastname: Park
authors.affiliation: National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Gary N
authors.initials: GN
authors.lastname: Sanden
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D001886:Bordetella pertussis / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004917:Erythromycin / Q000494:pharmacology*; D020459:Genes, rRNA / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014917:Whooping Cough / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D012338:RNA, Ribosomal, 23S; D004917:Erythromycin