In vivo confocal and multiphoton microendoscopy.
The ability to conduct high-resolution fluorescence imaging in internal organs of small animal models in situ and over time can make a significant impact in biomedical research. Toward this goal, we developed a real-time confocal and multiphoton endoscopic imaging system. Using 1-mm-diameter endoscopes based on gradient index lenses, we demonstrate video-rate multicolor multimodal imaging with cellular resolution in live mice.
Kim
Pilhan
P
Harvard Medical School and Massachusetts General Hospital, Wellman Center for Photomedicine, 50 Blossom St., BAR 818, Boston, Massachusetts 02114, USA.
Puoris'haag
Mehron
M
Côté
Daniel
D
Lin
Charles P
CP
Yun
Seok H
SH
D019723:Endoscopes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D018613:Microscopy, Confocal / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D008904:Miniaturization; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity