Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins.
Combined with preparative high-performance liquid chromatography, high-speed counter-current chromatography was employed for isolation and purification of saponins from Gypsophila paniculata L. n-Hexane-n-butanol-methanol-0.02% TFA (1:9:1:9, v/v) was employed as solvent system and 210 nm was chosen as the wavelength of ultraviolet detection for the first time. The research tried to compare HSCCC with prep-HPLC, and further integrated their advantages to improve separation efficiency. Five known triterpene saponins were identified by 13C NMR and ESI-MS and their purities were all above 96%. The results demonstrated that adopted method was a feasible, economical and efficient technique for rapid preparative isolation of saponins.
Yao
Shun
S
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.
Luo
Jianguang
J
Huang
Xuefeng
X
Kong
Lingyi
L
D020001:1-Butanol; D029748:Caryophyllaceae / Q000737:chemistry*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D006586:Hexanes; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol; D012503:Saponins / Q000302:isolation & purification*; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification
D006586:Hexanes; D012503:Saponins; D012997:Solvents; D014315:Triterpenes; C026385:n-hexane; D020001:1-Butanol; D000432:Methanol