title: In vitro inhibition of human influenza A virus infection by fruit-juice concentrate of Japanese plum (Prunus mume SIEB. et ZUCC).
abstract: Using a plaque reduction assay, treatment of human influenza A viruses with the fruit-juice concentrate of Japanese plum (Prunus mume SIEB. et ZUCC) showed strong in vitro anti-influenza activity against human influenza A viruses before viral adsorption, but not after viral adsorption, with 50% inhibitory concentration (IC50) values against A/PR/8/34 (H1N1) virus, A/Aichi/2/68 (H3N2) virus and A/Memphis/1/71 (H3N2) virus of 6.35+/-0.17, 2.84+/-1.98 and 0.53+/-0.10 microg/ml, respectively. The plum-juice concentrate exhibited hemagglutination activity toward guinea pig erythrocytes. Its hemagglutination activity was inhibited by the monosaccharide N-acetylneuraminic acid and a sialoglycoprotein (fetuin), but not by the other tested monosaccharides (mannose, galactose, glucose and N-acetylglucosamine), suggesting the presence of a lectin-like molecule(s) in the Japanese plum-juice concentrate. Our findings suggest that the fruit-juice concentrate of Japanese plum may prevent and reduce infection with human influenza A virus, possibly via inhibition of viral hemagglutinin attachment to host cell surfaces by its lectin-like activity.
authors.affiliation: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, 1200 Matsumoto, Kasugai, Aichi 487-8501, Japan.
authors.forename: Sangchai
authors.initials: S
authors.lastname: Yingsakmongkon
authors.forename: Daisei
authors.initials: D
authors.lastname: Miyamoto
authors.forename: Nongluk
authors.initials: N
authors.lastname: Sriwilaijaroen
authors.forename: Kimie
authors.initials: K
authors.lastname: Fujita
authors.forename: Kosai
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Wipawee
authors.initials: W
authors.lastname: Jampangern
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hiramatsu
authors.forename: Chao-Tan
authors.initials: CT
authors.lastname: Guo
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Sawada
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Hidari
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Morihiro
authors.initials: M
authors.lastname: Ito
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Ito
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001628:Beverages; D002460:Cell Line; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000821:virology; D005638:Fruit / Q000737:chemistry*; D006168:Guinea Pigs; D006387:Hemagglutination, Viral / Q000187:drug effects; D009980:Influenza A virus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000472:pathogenicity; D009005:Monosaccharides / Q000494:pharmacology; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry*; D010948:Viral Plaque Assay
chemical_list: D009005:Monosaccharides; D010936:Plant Extracts; D009439:Neuraminidase