title: Urotensin II and urotensin II-related peptide activate somatostatin receptor subtypes 2 and 5.
abstract: The UII and urotensin II-related peptide (URP) genes belong to the same superfamily as the somatostatin gene. It has been previously shown that somatostatin activates the UII-receptor (UTR). In contrast, the possible interaction between UII and URP and somatostatin receptors has remained scarcely analyzed. Herein, we have investigated the effects of UII and URP on cell proliferation and free cytosolic Ca2+ concentration ([Ca2+]i) in CHO-K1 cells stably expressing the porcine somatostatin receptor subtypes sst2 and sst5. Results show that both UII and URP induce stimulation of cell proliferation mediated by sst2 receptors and UII provokes inhibition of cell proliferation mediated by sst5 receptors. UII and URP also provoked an increase of [Ca2+]i in both sst2- and sst5-transfected cells. Together, our present data demonstrate that UII and URP directly activate sst2 and sst5 and thus mimic the effect of somatostatin on its cognate receptors.
authors.affiliation: Department of Cell Biology, Physiology and Immunology, University of Cordoba, 14014 Cordoba, Spain. bc1mapom@uco.es
authors.forename: Maria M
authors.initials: MM
authors.lastname: Malagon
authors.forename: Marcelo
authors.initials: M
authors.lastname: Molina
authors.forename: Manuel D
authors.initials: MD
authors.lastname: Gahete
authors.forename: Mario
authors.initials: M
authors.lastname: Duran-Prado
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Martinez-Fuentes
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Alcain
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Tonon
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.forename: Hubert
authors.initials: H
authors.lastname: Vaudry
authors.forename: Justo P
authors.initials: JP
authors.lastname: Castaño
authors.forename: Rafael
authors.initials: R
authors.lastname: Vazquez-Martinez
mesh_terms: D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D036361:Peptide Hormones / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D013004:Somatostatin / Q000819:agonists / Q000378:metabolism; D013552:Swine; D014508:Urea / Q000031:analogs & derivatives / Q000378:metabolism; D014579:Urotensins / Q000378:metabolism*
chemical_list: D036361:Peptide Hormones; D011804:Quinolines; D017481:Receptors, Somatostatin; D014579:Urotensins; D013004:Somatostatin; D014508:Urea; C094573:somatostatin receptor 5; C029677:urotensin II; C093733:somatostatin receptor 2; D002118:Calcium; C493249:1-(2-(4-benzyl-4-hydroxypiperidin-1-yl)ethyl)-3-(2-methylquinolin-4-yl)urea