title: Simultaneous determination of 10 active components in traditional Chinese medicine "YIGONG" capsule by RP-HPLC-DAD.
abstract: A simple, reliable and accurate method for the simultaneous separation and determination of 10 active components (psoralen, isopsoralen, emodin, oleanolic acid, stachydrine hydrochloride, ammonium glycyrrhizinate, glycyrrhizinate, schizandrol, imperatorin and isoimperatorin) in traditional Chinese medicine "YIGONG" capsule was developed using reverse phase high-performance liquid chromatography (RP-HPLC) coupled with diode array detection. The chromatographic separation was performed on a Eurospher C(18) column (250 mm x 4.6 mm i.d. with 5.0 microm particle size) with a acetonitrile-water gradient containing 0.5% (v/v) aqueous phosphoric acid as mobile phase. Two detection wavelengths (210 and 250 nm) were utilized for the quantitative analysis due to the different UV spectra of these components. Good linear behaviors over the investigated concentration ranges were observed with the values of R(2) higher than 0.999 for all the analytes. The recoveries, measured at three concentration levels, varied from 95.0 to 105.3%. The validated method was successfully applied to the simultaneously determination of these active components in "YIGONG" capsule from different production batches.
authors.affiliation: College of Pharmacy, Tongji Medical Center of Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Feng
authors.forename: Ya-Ling
authors.initials: YL
authors.lastname: Cai
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Ruan
mesh_terms: D002214:Capsules / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D012996:Solutions