title: Sodium nitroprusside as a nitric oxide donor in a rat intestinal ischemia-reperfusion model.
abstract: OBJECTIVE
  The aim of this study was to investigate the efficacy of sodium nitroprusside in the reduction of the intestinal ischemia-reperfusion injury as a nitric oxide donor after intraperitoneal administration.
  
  
  METHODS
  The histopathological examinations and tissue malonyldialdehyde levels of 35 Wistar albino rats that were subjected to ischemia-reperfusion, were performed in 5 groups. The groups include Control, Ischemia -reperfusion, Sodium nitroprusside, NG-Nitro-L-Arginine Methyl Ester (L-NAME) and Sodium nitroprusside+L-NAME. Each rat was subjected to ischemia for 40 minutes and reperfusion for 30 minutes, except the control group. The medications were done intraperitoneally as saline 4 ml/kg, Sodium nitroprusside 5 mg/kg, L-NAME 10 mg/kg just before reperfusions.
  
  
  RESULTS
  Significant tissue injury in histological sections and an increase in tissue levels of Malonyldialdehyde was detected in the I/R group. The efficacy of intraperitoneal administration of Sodium nitroprusside in both Sodium nitroprusside alone and Sodium nitroprusside+L-NAME groups was found statistically significant for the reducing of injury scores (p<0.05). The difference between the Ischemia/reperfusion and Sodium nitroprusside groups was found statistically significant as in the Ischemia/reperfusion and Sodium nitroprusside+L-NAME groups due to the tissue Malonyldialdehyde levels (p<0.05). There was no statistical difference between Ischemia/reperfusion and L-NAME groups.
  
  
  CONCLUSIONS
  Ischemia/reperfusion induced injury might be reduced by the intraperitoneal administration of Sodium nitroprusside, even in the presence of L-NAME, in the rat intestinal model.
authors.affiliation: General Surgery, Zonguldak Karaelmas University, Turkey. auemre@gmail.com
authors.forename: Ali
authors.initials: A
authors.lastname: Emre
authors.forename: Orhan
authors.initials: O
authors.lastname: Bayram
authors.forename: Bulent
authors.initials: B
authors.lastname: Salman
authors.forename: Sevim
authors.initials: S
authors.lastname: Ercan
authors.forename: Ziya
authors.initials: Z
authors.lastname: Anadol
authors.forename: Okhan
authors.initials: O
authors.lastname: Akin
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007422:Intestines / Q000098:blood supply* / Q000473:pathology; D007511:Ischemia / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000473:pathology*
chemical_list: D020030:Nitric Oxide Donors; D009599:Nitroprusside; D008315:Malondialdehyde; D019331:NG-Nitroarginine Methyl Ester