title: Effect of exogenous vitamin E on proliferation and cytokine production in peripheral blood mononuclear cells from patients with tuberculosis.
abstract: Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested the effect of exogenous vitamin E on proliferation and cytokine production of peripheral blood mononuclear cells (PBMC) from TB patients and healthy purified protein derivative (PPD)+ volunteers. Proliferation was stimulated with mycobacterial antigen (PPD) and evaluated by the incorporation of tritiated thymidine in PBMC cultured with or without 50 microm-vitamin E for 6 d. Cytokine production (IL-2 and interferon (IFN)-gamma) was determined by intracellular cytokine staining and by ELISA in the supernatant of PBMC stimulated for 24 h with phytohaemagglutinin or PPD. Our results show that culture with vitamin E increased (P < or = 0.05 ) the antigen-induced proliferation of PBMC in TB patients but not in healthy PPD+ volunteers. No significant changes in the number of cytokine-producing cells or in the production of IFN-gamma were observed with vitamin E treatment. These results indicate that vitamin E may enhance the antigen-specific in vitro response of PBMC from TB patients.
authors.affiliation: Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo, A.C., Sonora, México. jhdez@ciad.mx
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
authors.forename: Adriana
authors.initials: A
authors.lastname: Garibay-Escobar
authors.forename: Andrés
authors.initials: A
authors.lastname: Mendoza-Mendoza
authors.forename: Araceli
authors.initials: A
authors.lastname: Pinelli-Saavedra
authors.forename: Olivia
authors.initials: O
authors.lastname: Valenzuela
mesh_terms: D000328:Adult; D000368:Aged; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000276:immunology; D024505:Tocopherols / Q000097:blood / Q000494:pharmacology*; D014373:Tuberculin / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000276:immunology*
chemical_list: D016207:Cytokines; D010835:Phytohemagglutinins; D014373:Tuberculin; D007371:Interferon-gamma; D024505:Tocopherols