Cellular phenotypes in the abomasal mucosa and abomasal lymph nodes of goats infected with Haemonchus contortus.
The distribution of T-cell subsets (CD2, CD4, CD8, and gammadelta) and B cells (IgM) was examined at 3, 6, 10 and 13 days post-infection (dpi) in the abomasal mucosa and abomasal lymph nodes of goats primarily infected with Haemonchus contortus. In the abomasal mucosa a mild (3 and 6 dpi) or marked (10 and 13 dpi) increase of T cells, particularly CD4+ and gammadelta+ lymphocytes, was observed, whereas the increase in CD8+ cells was less pronounced. B cells and IgG+ plasma cells also showed a marked increase in the abomasal mucosa at 10 and 13 dpi. The abomasal lymph nodes showed an increase in size, particularly at 10 and 13 dpi, and a decrease in the proportion of T cells, particularly CD8+ lymphocytes, due to the increased proportion of B cells. The proportion of CD4+ and gammadelta+ lymphocytes did not change significantly during the infection in the abomasal lymph nodes, but their absolute numbers were augmented as a result of the enlargement of the nodes. The results revealed a strong cellular and humoral immune response during the early post-infection stages. However, as indicated by the worm burdens, this rapid host response was unable to induce larval expulsion.
Pérez
J
J
Departamento de Anatomía y Anatomía Patológica Comparadas, Edificio de Sanidad Animal, Campus de Rabanales, Ctra. Madrid-Cádiz, Km 396, 14014 Córdoba, Spain. an1pearj@uco.es
Zafra
R
R
Buffoni
L
L
Hernández
S
S
Cámara
S
S
Martínez-Moreno
A
A
D000018:Abomasum / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D002452:Cell Count / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D015511:Goat Diseases / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D006041:Goats; D006188:Haemonchiasis / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D006189:Haemonchus / Q000302:isolation & purification* / Q000502:physiology; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008198:Lymph Nodes / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D008214:Lymphocytes / Q000276:immunology / Q000469:parasitology / Q000473:pathology*; D008297:Male; D009092:Mucous Membrane / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000469:parasitology / Q000473:pathology