Use of real-time PCR to detect Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' in the saliva and salivary glands of haemoplasma-infected cats.
Feline haemoplasma infection can cause haemolytic anaemia. The natural method of transmission of haemoplasmas between cats is currently unknown but the nature of some of the risk factors for infection suggests that saliva may act as a mode of transmission. The aim of this study was to determine if Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (CMhm) DNAs could be amplified from saliva and salivary gland samples collected from haemoplasma-infected cats.
Dean
Rachel S
RS
School of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, UK. rachel.dean@aht.org.uk
Helps
Chris R
CR
Gruffydd Jones
Timothy J
TJ
Tasker
Séverine
S
D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000635:transmission; D002415:Cats; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005784:Gene Amplification; D009174:Mycoplasma / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012463:Saliva / Q000382:microbiology*; D012469:Salivary Glands / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors
D004269:DNA, Bacterial