title: Suppressive effects of Anoectochilus formosanus extract on osteoclast formation in vitro and bone resorption in vivo.
abstract: Anoectochilus formosanus, a plant native to Taiwan, is used as a folk medicine. It was found that oral administration of A. formosanus extract (AFE) (500 mg/kg) for 4 weeks suppressed bone weight loss and trabecular bone loss in ovariectomized mice, an experimental model of osteoporosis. Although AFE at 12.5 and 25 mug/ml inhibited osteoclast formation in co-culture of osteoblasts and bone marrow cells, AFE did not inhibit the formation of osteoclast progenitor cells and preosteoclast cells in bone marrow cells and RAW264 cells. However, AFE (at 12.5 and 25 microg/ml) decreased RANKL expression. These results suggested that AFE might suppress the bone loss caused by estrogen deficiency through suppression of RANKL expression required for osteoclast formation.
authors.affiliation: Laboratory of Nutraceuticals and Functional Foods Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minatoku, Tokyo 108-8477, Japan.
authors.forename: Kikuko
authors.initials: K
authors.lastname: Masuda
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Koyama
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Je-Tae
authors.initials: JT
authors.lastname: Woo
authors.forename: Tomio
authors.initials: T
authors.lastname: Nishimura
authors.forename: Kazunaga
authors.initials: K
authors.lastname: Yazawa
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001862:Bone Resorption / Q000188:drug therapy* / Q000473:pathology*; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D005269:Femur / Q000187:drug effects / Q000473:pathology; D051379:Mice; D029595:Orchidaceae; D009929:Organ Size / Q000187:drug effects; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010052:Ovariectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D053245:RANK Ligand / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects
chemical_list: D010936:Plant Extracts; D053245:RANK Ligand; D002117:Calcitriol