title: Neonatal hyperoxia enhances the inflammatory response in adult mice infected with influenza A virus.
abstract: BACKGROUND
  Lungs of adult mice exposed to hyperoxia as newborns are simplified and exhibit reduced function much like that observed in people who had bronchopulmonary dysplasia (BPD) as infants. Because survivors of BPD also show increased risk for symptomatic respiratory infections, we investigated how neonatal hyperoxia affected the response of adult mice infected with influenza A virus infection.
  
  
  OBJECTIVE
  To determine whether neonatal hyperoxia increased the severity of influenza A virus infection in adult mice.
  
  
  METHODS
  Adult female mice exposed to room air or hyperoxia between Postnatal Days 1 and 4 were infected with a sublethal dose of influenza A virus.
  
  
  RESULTS
  The number of macrophages, neutrophils, and lymphocytes observed in airways of infected mice that had been exposed to hyperoxia as neonates was significantly greater than in infected siblings that had been exposed to room air. Enhanced inflammation correlated with increased levels of monocyte chemotactic protein-1 (CCL2) in lavage fluid, whereas infection-associated changes in IFN-gamma, IL-1beta, IL-6, tumor necrosis factor-alpha, KC, granulocyte-macrophage colony-stimulating factor, and macrophage inflammatory protein-1alpha, and production of virus-specific antibodies, were largely unaffected. Increased mortality of mice exposed to neonatal hyperoxia occurred by Day 14 of infection, and was associated with persistent inflammation and fibrosis.
  
  
  CONCLUSIONS
  These data suggest that the disruptive effect of hyperoxia on neonatal lung development also reprograms key innate immunoregulatory pathways in the lung, which may contribute to exacerbated pathology and poorer resistance to respiratory viral infections typically seen in people who had BPD.
authors.affiliation: Department of Pediatrics, Box 850, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA. michael_oreilly@urmc.rochester.edu
authors.forename: Michael A
authors.initials: MA
authors.lastname: O'Reilly
authors.forename: Shauna H
authors.initials: SH
authors.lastname: Marr
authors.forename: Min
authors.initials: M
authors.lastname: Yee
authors.forename: Sharon A
authors.initials: SA
authors.lastname: McGrath-Morrow
authors.forename: B Paige
authors.initials: BP
authors.lastname: Lawrence
mesh_terms: D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D001997:Bronchopulmonary Dysplasia / Q000150:complications / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D018496:Hyperoxia / Q000276:immunology* / Q000473:pathology; D007231:Infant, Newborn; D009980:Influenza A virus / Q000276:immunology*; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000150:complications / Q000276:immunology* / Q000473:pathology; D011014:Pneumonia / Q000276:immunology / Q000473:pathology / Q000821:virology; D011650:Pulmonary Alveoli / Q000473:pathology