title: Abnormal nuclear structures (micronuclei, nucleoplasmic bridges, and nuclear buds) in a pleomorphic giant cell carcinoma of the stomach.
abstract: A rare case of pleomorphic giant cell carcinoma of the stomach in a 70-year-old man is reported. Characteristic microscopic findings included a general lack of architectural cohesiveness, aggregates of mononucleated or multinucleated giant cells, extensive areas of coagulative necrosis, and numerous mitoses. Immunohistochemically, tumor cells displayed cytoplasmic immunoreactivity for cytokeratin AE1/AE3 as well as overexpression of p53 and Ki-67. Electron microscopy revealed paranuclear tonofilaments bundles in giant cells confirming their epithelial nature. Furthermore, giant cells contained two or more nuclei with heterogeneous size, nucleoplasmic bridges, nuclear buds, and micronuclei. Similar abnormal nuclear structures have been closely related to breakage-fusion-bridge type of mitotic disturbances in tumor cell lines, and have not been previously reported in a human tumor.
authors.affiliation: Department of Human Pathology, University Hospital, Messina, Italy. rosariocaruso@tin.it
authors.forename: Rosario Alberto
authors.initials: RA
authors.lastname: Caruso
authors.forename: Francesco
authors.initials: F
authors.lastname: Fedele
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Consolo
authors.forename: Carmelo
authors.initials: C
authors.lastname: Luigiano
authors.forename: Antonio
authors.initials: A
authors.lastname: Venuti
authors.forename: Vittorio
authors.initials: V
authors.lastname: Cavallari
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D018286:Carcinoma, Giant Cell / Q000737:chemistry / Q000601:surgery / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D053145:Cell Nucleus Size; D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D017809:Fatal Outcome; D019085:Fluorescent Antibody Technique, Direct; D015726:Giant Cells / Q000737:chemistry / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D013274:Stomach Neoplasms / Q000737:chemistry / Q000601:surgery / Q000648:ultrastructure*
chemical_list: D014408:Biomarkers, Tumor