[Detection of a surface sheath on Azospirillum brasilense polar flagellum].
The presence of a polysaccharide sheath on the surface of the polar flagellum of Azospirillum brasilense was revealed by immunoelectron microscopy and immunodiffusion analysis with strain-specific antibodies to lipopolysaccharides (LPS). The antigenic identity of A. brasilense Sp245 sheath material and one of the two O-specific polysaccharides of its somatic LPS was demonstrated. The screening effect of the sheath in respect to flagellin was determined by agglutination tests and by the inhibition of azospirilla motility in liquid and semisolid agarized media caused by strain-specific antibodies to LPS; no pronounced effect of genus-specific antibodies to flagellin was observed.
Burygin
G L
GL
Shirokov
A A
AA
Shelud'ko
A V
AV
Katsy
E I
EI
Shchegolev
S Iu
SIu
Matora
L Iu
LIu
D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D015806:Azospirillum brasilense / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D016667:Bacterial Capsules / Q000648:ultrastructure; D005407:Flagella / Q000737:chemistry / Q000648:ultrastructure*; D005408:Flagellin / Q000276:immunology; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D008124:Locomotion; D008854:Microscopy, Electron; D019081:O Antigens / Q000032:analysis / Q000276:immunology
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens; D005408:Flagellin