title: Expression of interleukin-8 and monocyte chemotactic protein 1 in women with endometriosis.
abstract: OBJECTIVE
  To investigate the expression and localization of interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) in women with and without endometriosis.
  
  
  METHODS
  Comparative immunohistochemical study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Ectopic (n = 24) and homologous eutopic endometrium (n = 24) from women with endometriosis and endometrium from women without endometriosis (n = 27) were used for immunohistochemical analysis of IL-8 and MCP-1.
  
  
  METHODS
  Tissue sections were immunostained with antihuman IL-8 and MCP-1 antibodies.
  
  
  METHODS
  Microscopic evaluation to assess the presence and localization of IL-8 and MCP-1 throughout the menstrual cycle in both eutopic endometrial and endometriotic tissues of women with endometriosis and comparison with normal endometrium.
  
  
  RESULTS
  In normal endometrium, secretory phase samples expressed higher levels of epithelial IL-8 than in proliferative phase samples. Epithelial MCP-1 expression was similar in both proliferative and secretory phases. Proliferative phase samples showed higher epithelial IL-8 and MCP-1 expressions in eutopic endometrium of women with endometriosis compared with that of normal women. Immunoreactivities of both chemokines were significantly increased in the epithelial cells of ectopic endometrial tissues compared with those of normal endometrium.
  
  
  CONCLUSIONS
  These findings suggest that IL-8 and MCP-1 may be involved in the pathogenesis of endometriosis.
authors.affiliation: Department of Obstetrics & Gynecology, Ege University School of Medicine, Bornova, Izmir, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Ulukus
authors.forename: E Cagnur
authors.initials: EC
authors.lastname: Ulukus
authors.forename: Ege N
authors.initials: EN
authors.lastname: Tavmergen Goker
authors.forename: Erol
authors.initials: E
authors.lastname: Tavmergen
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zheng
authors.forename: Aydin
authors.initials: A
authors.lastname: Arici
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000032:analysis*; D004715:Endometriosis / Q000276:immunology* / Q000473:pathology; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000032:analysis*; D008875:Middle Aged; D012189:Retrospective Studies; D017154:Stromal Cells / Q000276:immunology
chemical_list: C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D016209:Interleukin-8