[Effect of Bushen Yiqi Hexue recipe on endometrial P21 expression of embryo implantation dysfunctional mice].
OBJECTIVE
To observe the effect of Bushen Yiqi Hexue recipe on endometrial P21 and PCNA expression of embryo implantation dysfunctional mice.


METHODS
The expression of P21 protein was analyzed by means of immunoblot and immunohistochemical method. The expression of PCNA protein was analyzed by means of immunohistochemical method.


RESULTS
The expression and disappearance of P21 and PCNA protein in endometrium of embryo implantation dysfunctional mice lagged behind in a temporal and cell specific manner. Bushenyiqihexue recipe could improve the expression of P21 and PCNA protein in a temporal and cell specific manner, and then improve the decidual reaction of endometrium of embryo implantation dysfunctional mice.


CONCLUSIONS
Improving the expression of P21 and PCNA protein in endometrium and eventually regulating endometrium decidual reaction is one of the mechanisms of improved embryo implantaion by Bushen Yiqi Hexue recipe.
Huang
Dong-Mei
DM
Institute of Integrated Traditional and Western Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Huang
Guang-Yin
GY
Xu
Li-Jun
LJ
Zhang
Ming-Min
MM
D000818:Animals; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen