title: Anamnestic antibody response after viral challenge in monkeys immunized with dengue 2 recombinant fusion proteins.
abstract: The suitability of dengue 2 envelope domain III recombinant fusion proteins [(fusion (PD5) and insertion (PD3) variants)] for inducing functional antibodies and a protective immune response in nonhuman primates has been reported. However, the evaluation of the antibody response after immunization did not correlate with the protection data as measured by viremia detection. Here, we characterized the anamnestic immune response after viral challenge in monkeys immunized with the dengue 2 recombinant proteins in an attempt to define correlates of protection useful for vaccine studies. Monkeys immunized with PD5 (most protected group) exhibited an earlier increase in the anti-DENV-2 IgM response after challenge compared to control animals. Hemagglutination-inhibiting (HAI) antibodies were increased significantly earlier in PD5-immunized animals compared to those immunized with PD3. The fully protected monkeys showed the earliest HAI antibody response. These results underline the usefulness of the anamnestic antibody response for supporting protection data. The induction of an early HAI and IgM antibody response after challenge suggest a protective role against dengue virus (DENV) infection in monkeys, supporting their use as correlates of protection in vaccine studies.
authors.affiliation: PAHO/WHO Collaborating Center for Viral Diseases, Department of Virology, Pedro Kourí Tropical Medicine Institute IPK, Autopista Novia del Mediodía, km 6 1/2, PO Box 601, Marianao 13, Havana, Cuba.
authors.forename: Lidice
authors.initials: L
authors.lastname: Bernardo
authors.forename: Lisset
authors.initials: L
authors.lastname: Hermida
authors.forename: Jorge
authors.initials: J
authors.lastname: Martin
authors.forename: Mayling
authors.initials: M
authors.lastname: Alvarez
authors.forename: Irina
authors.initials: I
authors.lastname: Prado
authors.forename: Carlos
authors.initials: C
authors.lastname: López
authors.forename: Rafael
authors.initials: R
authors.lastname: Martínez
authors.forename: Rosmari
authors.initials: R
authors.lastname: Rodríguez-Roche
authors.forename: Aida
authors.initials: A
authors.lastname: Zulueta
authors.forename: Laura
authors.initials: L
authors.lastname: Lazo
authors.forename: Delfina
authors.initials: D
authors.lastname: Rosario
authors.forename: Gerardo
authors.initials: G
authors.lastname: Guillén
authors.forename: María G
authors.initials: MG
authors.lastname: Guzmán
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D003715:Dengue / Q000276:immunology / Q000517:prevention & control*; D053059:Dengue Vaccines / Q000235:genetics / Q000276:immunology*; D003716:Dengue Virus / Q000235:genetics / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D007075:Immunoglobulin M / Q000096:biosynthesis; D008252:Macaca fascicularis; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014766:Viremia / Q000276:immunology / Q000517:prevention & control
chemical_list: D000914:Antibodies, Viral; D053059:Dengue Vaccines; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins