Quantification of plasma reduced glutathione, oxidized glutathione and plasma total glutathione in healthy cats.
Glutathione is an important intracellular tripeptide with multiple functions. Abnormal glutathione metabolism is thought to play an important role in various diseases of cats. However, no data regarding concentration of plasma glutathione are available for domestic cats. This study discusses the development of a rapid, simple high pressure liquid chromatography method for measurement of reduced glutathione (GSH), oxidized glutathione (GSSH) and total glutathione (GSHt) in plasma, for the purpose of establishing baseline data for future studies. The mean concentrations of GSH, GSSH and GSHt were 4.51+/-1 microM; 19.44+/-3.79 microM (expressed as GSH equivalent) and 23.59+/-3.89 microM, respectively. This is the first report of plasma glutathione concentrations in this species.
Denzoin
Laura A
LA
Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina. laura@vet.unicen.edu.ar
Franci
Rodolfo J
RJ
Tapia
Maria O
MO
Soraci
Alejandro L
AL
D000818:Animals; D002415:Cats / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005260:Female; D005978:Glutathione / Q000097:blood*; D019803:Glutathione Disulfide / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D008297:Male; D010949:Plasma / Q000378:metabolism*; D012016:Reference Values; D013045:Species Specificity
D005982:Glutathione Transferase; D005978:Glutathione; D019803:Glutathione Disulfide