title: Certification of reference materials for detection of the human prothrombin gene G20210A sequence variant.
abstract: BACKGROUND
  There is a need for reference materials (RMs) in the field of genetic testing for verification of test results obtained in patients and probands. For the frequent genetic variation G20210A in the prothrombin gene, it has been shown that purified plasmids containing the gene fragment harbouring the mutation constitute good candidate RMs.
  
  
  METHODS
  Plasmid-type RMs were characterised for homogeneity, stability, sequence identity and fitness for purpose. Their certification required the use of different real-time PCR methods for genotyping and quantification of the plasmid copy number.
  
  
  RESULTS
  Homogeneity, stability and fitness for the purpose of the plasmids could be demonstrated. The long-term stability (up to 24 months) of the materials was confirmed by highly sensitive and specific quantitative real-time PCR methods.
  
  
  CONCLUSIONS
  New types of certified RMs (CRMs) for genetic testing of the human prothrombin gene G20210A sequence variant are available. Their fitness for purpose was demonstrated and no evidence was found that they would not work with other methods as long as these are targeting the whole or parts of the prothrombin gene fragment inserted into the plasmids. The described CRMs support the efforts of the international community in development, validation and harmonisation of tests for molecular genetic testing.
authors.affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Geel, Belgium. david.gancberg@ec.europa.eu
authors.forename: David
authors.initials: D
authors.lastname: Gancberg
authors.forename: Philippe
authors.initials: P
authors.lastname: Corbisier
authors.forename: Nele
authors.initials: N
authors.lastname: Meeus
authors.forename: Janos
authors.initials: J
authors.lastname: Marki-Zay
authors.forename: Christine
authors.initials: C
authors.lastname: Mannhalter
authors.forename: Heinz
authors.initials: H
authors.lastname: Schimmel
mesh_terms: D000483:Alleles; D001774:Blood Chemical Analysis / Q000379:methods / Q000592:standards*; D002138:Calibration; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008957:Models, Genetic; D009930:Organic Chemicals / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011516:Prothrombin / Q000235:genetics*; D012015:Reference Standards; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature
chemical_list: D009930:Organic Chemicals; C099476:PicoGreen; D011516:Prothrombin