VE-cadherin and beta-catenin binding dynamics during histamine-induced endothelial hyperpermeability.
Beta-catenin plays an important role in the regulation of vascular endothelial cell-cell adhesions and barrier function by linking the VE-cadherin junction complex to the cytoskeleton. The purpose of this study was to evaluate the effect of beta-catenin and VE-cadherin interactions on endothelial permeability during inflammatory stimulation by histamine. We first assessed the ability of a beta-catenin binding polypeptide known as inhibitor of beta-catenin and T cell factor (ICAT) to compete beta-catenin binding to VE-cadherin in vitro. We then overexpressed recombinant FLAG-ICAT in human umbilical vein endothelial cells (HUVECs) to study its impact on endothelial barrier function controlled by cell-cell adhesions. The binding of beta-catenin to VE-cadherin was quantified before and after stimulation with histamine along with measurements of transendothelial electrical resistance (TER) and apparent permeability to albumin (P(a)) under the same conditions. The results showed that ICAT bound to beta-catenin and competitively inhibited binding of the VE-cadherin cytoplasmic domain to beta-catenin in a concentration-dependent manner. Overexpression of FLAG-ICAT in endothelial cell monolayers did not affect their basal permeability properties, as indicated by unaltered TER and P(a); however, the magnitude and duration of histamine-induced decreases in TER were significantly augmented. Likewise, the increase in P(a) in the presence of histamine was exacerbated. Overexpression of FLAG-ICAT also significantly decreased the level of beta-catenin-associated VE-cadherin following histamine stimulation. Taken together, these data suggest that inflammatory agents like histamine cause a transient and reversible disruption of binding between beta-catenin and VE-cadherin, during which endothelial permeability is elevated.
Guo
Mingzhang
M
Department of Surgery, University of California-Davis School of Medicine, Sacramento, CA 95817, USA.
Breslin
Jerome W
JW
Wu
Mack H
MH
Gottardi
Cara J
CJ
Yuan
Sarah Y
SY
D048868:Adaptor Proteins, Signal Transducing; D000418:Albumins / Q000493:pharmacokinetics; D015703:Antigens, CD / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D042783:Endothelial Cells; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D005786:Gene Expression Regulation; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D010539:Permeability / Q000187:drug effects; D011485:Protein Binding; D012097:Repressor Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism*; D000099152:Cadherin 5
D048868:Adaptor Proteins, Signal Transducing; D000418:Albumins; D015703:Antigens, CD; D015820:Cadherins; D018797:Cell Cycle Proteins; D006632:Histamine; D047908:Intracellular Signaling Peptides and Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D051176:beta Catenin; D000099152:Cadherin 5; C411982:CTNNBIP1 protein, human; C498785:Ctnnbip1 protein, mouse