title: Simultaneous determination of five beta-lactam antibiotics (cefepim, ceftazidim, cefuroxim, meropenem and piperacillin) in human plasma by high-performance liquid chromatography with ultraviolet detection.
abstract: Monitoring of plasma antibiotic drugs is useful for better clinical management in infected patients, particularly in intensive care units. A simple and sensitive high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection has been developed and validated for simultaneous quantification of five beta-lactam antibiotics in human plasma: cefepim, ceftazidim, cefuroxim, meropenem and piperacillin. The plasma sample, after spiked with ceforanid as an internal standard (IS), was submitted to a solid-phase extraction (SPE) prior to HPLC analysis. A chromatographic separation was achieved on a C8 symmetry column with a mobile phase consisting of an acetonitrile and phosphate buffer (pH 7.4) mixture in a gradient mode. Detection was carried out at a wavelength between 200 and 400 nm. The method developed was linear over the concentration range of 2.5-60 microg/mL for each antibiotic in the plasma samples. Accuracy ranged from 93.2 to 107.1% and precision was between 0.9 and 12.2%. The method has been applied to plasma samples obtained from patients treated with beta-lactam antibiotics and is appropriated for easy determination of plasma concentrations for therapeutic monitoring applications.
authors.affiliation: Laboratoire de Toxicologie Clinique et de Toxicologie Médico-Légale, Centre Hospitalier Universitaire Sart-Tilman B 35, B 4000 Liège, Belgium. Raphael.denooz@chu.ulg.ac.be <Raphael.denooz@chu.ulg.ac.be>
authors.forename: Raphaël
authors.initials: R
authors.lastname: Denooz
authors.forename: Corinne
authors.initials: C
authors.lastname: Charlier
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood*; D000077723:Cefepime; D002442:Ceftazidime / Q000097:blood; D002444:Cefuroxime / Q000097:blood; D002511:Cephalosporins / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D006801:Humans; D000077731:Meropenem; D010878:Piperacillin / Q000097:blood; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000097:blood; D047090:beta-Lactams / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D013845:Thienamycins; D047090:beta-Lactams; D000077723:Cefepime; D002442:Ceftazidime; D000077731:Meropenem; D002444:Cefuroxime; D010878:Piperacillin