[Separation and purification of flavonoids from chrysanthemum indicum with macroporous resin].
OBJECTIVE
A method for separation and purification of flavonoids from C. indicum with macroporous resin was studied.


METHODS
By using C. indicum in Guizhou as the materials and with the content and recovery rate of flavonoids as indexes, the static and dynamic adsorption tests were employed to investigated effects and affective factors of separation and purification of flavonoids from C. indicum with macroporous resin.


RESULTS
Results show that the static adsorption capacity of AB-8 type resin was 114.65 mg x g(-1), the static elution ratio were 94.9%, the dynamic adsorption capacity of AB-8 type resin was 94.5 mg x g(-1), the recovery rate was more than 92.6% and the purity of flavonoids was more than 90%. AB-8 type resin is the best for separating and purificating C. indicum in flavonoids.


CONCLUSIONS
The optimum conditions is AB-8 type macroporous resin, 70% alcohol as the eluant and 2 to approximately 3 times volume of the resin as the eluant volume, the ratio of flavonoids to the volume of the resin as 1:10.6, concentration of flavonoids of sample as 19.8 mg x mL(-1) and current velocity as 2 to approximately 3 mL x min(-1), pH value of sample as 4 to approximately 5. [Key words]' macroporous resin; Chrysanthemum indicum; flavonoids; separation; purification
Yu
Jian-Sheng
JS
Tongren Vocational Institute, Tongren 554300, China. yujiansheng@sohu.com
Yu
Jian-Ping
JP
D000327:Adsorption; D031202:Chrysanthemum / Q000737:chemistry*; D000431:Ethanol; D005419:Flavonoids / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods*
D005419:Flavonoids; D012117:Resins, Synthetic; D000431:Ethanol