title: Transplacental toxoplasmosis in a wild southern sea otter (Enhydra lutris nereis).
abstract: In September 2004, a neonatal sea otter pup was found alive on the beach in northern Monterey Bay, CA. Efforts to locate the mother were unsuccessful. Due to a poor prognosis for successful rehabilitation, the pup was euthanized. Postmortem examination revealed emaciation, systemic lymphadenopathy and a malformation of the left cerebral temporal lobe. On histopathology, free tachyzoites and tissue cysts compatible with Toxoplasma gondii were observed in the brain, heart, thymus, liver, lymph nodes and peri-umbilical adipose. The presence of T. gondii within host tissues was associated with lymphoplasmacytic inflammation and tissue necrosis. Immunofluorescent antibody tests using postmortem serum were positive for anti-T. gondii IgM and IgG (at 1:320 and 1:1280 serum dilution, respectively), but were negative for IgG directed against Sarcocystis neurona and Neospora caninum (<1:40 each). Brain immunohistochemistry revealed positive staining for tachyzoites and tissue cysts using antiserum raised to T. gondii, but not S. neurona or N. caninum. T. gondii parasite DNA was obtained from extracts of brain and muscle by PCR amplification using the diagnostic B1 locus. Restriction enzyme digestion followed by gel electrophoresis and DNA sequencing confirmed the presence of Type X T. gondii, the strain identified in the majority of southern sea otter infections.
authors.affiliation: California Department of Fish and Game-Office of Spill Prevention and Response, Marine Wildlife Veterinary Care and Research Center, 1451 Shaffer Road, Santa Cruz, CA 95060, United States. mmiller@ospr.dfg.ca.gov
authors.forename: Melissa
authors.initials: M
authors.lastname: Miller
authors.forename: Patricia
authors.initials: P
authors.lastname: Conrad
authors.forename: E R
authors.initials: ER
authors.lastname: James
authors.forename: Andrea
authors.initials: A
authors.lastname: Packham
authors.forename: Sharon
authors.initials: S
authors.lastname: Toy-Choutka
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Murray
authors.forename: David
authors.initials: D
authors.lastname: Jessup
authors.forename: Michael
authors.initials: M
authors.lastname: Grigg
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000835:Animals, Wild; D001921:Brain / Q000469:parasitology / Q000473:pathology; D005260:Female; D018445:Infectious Disease Transmission, Vertical / Q000662:veterinary*; D009206:Myocardium / Q000473:pathology; D010041:Otters; D011247:Pregnancy; D014124:Toxoplasmosis, Animal / Q000473:pathology / Q000635:transmission*