title: Development of the tracer-pulse method for adsorption studies of analyte mixtures in liquid chromatography utilizing mass spectrometric detection.
abstract: The tracer-pulse method provides the real adsorption data points directly from simple, straightforward calculations and is therefore a superior method for multicomponent adsorption isotherm determination in HPLC. Only one important problem has restricted its use so far: the tracer peaks are invisible using any conventional detection principle. We present a solution to this problem with an approach with a firm base in analytical chemistry, utilizing stable isotopes and mass spectrometric detection. The new approach was used for the determination of binary adsorption isotherms, and a systematic investigation was made of its main sources of error. With this modification, the tracer method can be a prime choice for future characterizations of multicomponent separation systems and of competitive drug binding studies.
authors.affiliation: Department of Physical and Analytical Chemistry, Uppsala University, BMC, Box 577, SE-751 23 Uppsala, Sweden.
authors.forename: Jörgen
authors.initials: J
authors.lastname: Samuelsson
authors.forename: Robert
authors.initials: R
authors.lastname: Arnell
authors.forename: Jarle S
authors.initials: JS
authors.lastname: Diesen
authors.forename: Julius
authors.initials: J
authors.lastname: Tibbelin
authors.forename: Alexander
authors.initials: A
authors.lastname: Paptchikhine
authors.forename: Torgny
authors.initials: T
authors.lastname: Fornstedt
authors.forename: Per J R
authors.initials: PJ
authors.lastname: Sjöberg