Non-invasive optical characterization of biomaterial mineralization.
Current approaches to study biomaterial mineralization are invasive and prevent dynamic characterization of this process within the same sample. Polarized light scattering spectroscopy (LSS) may offer a non-invasive alternative for assessing the levels of mineralization as well as some aspects of the organization of the mineral deposits. Specifically, we used LSS to characterize the formation of hydroxyapatite deposits on three types of silk films (water-annealed, methanol-treated and polyaspartic acid (PAA)-mixed) following 1, 3, 5 and 7 cycles of mineralization. We found that the total light scattering intensity provided a quantitative measure of the degree of mineralization as confirmed by thermal gravimetric analysis (TGA). The PAA-mixed silk films yielded the highest level of mineral deposition and the water-annealed ones the least, consistent with the beta sheet content of the films prior to the onset of mineralization. The wavelength dependence of the singly backscattered light was consistent with a self-affine fractal morphology of the deposited films within scales in the range of 150-300nm; this was confirmed by Fourier analysis of scanning electron microscopy (SEM) images of the corresponding films. The deposits of minerals in the water-annealed films were predominantly flake-like, with positively correlated density fluctuations (Hurst parameter, H>0.5), whereas methanol-treated and PAA-mixed silk films resulted in densely-packed, bulk mineral deposits with negatively correlated density fluctuations (H<0.5). Therefore, LSS could serve as a valuable tool for understanding the role of biomaterial properties in mineral formation, and, ultimately, for optimizing biomaterial designs that yield mineral deposits with the desired organization.
Gupta
Sharad
S
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
Hunter
Martin
M
Cebe
Peggy
P
Levitt
Jonathan M
JM
Kaplan
David L
DL
Georgakoudi
Irene
I
D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D002113:Calcification, Physiologic; D002122:Calcium Chloride / Q000737:chemistry; D002152:Calorimetry, Differential Scanning / Q000379:methods; D003460:Crystallization; D005349:Fibroins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D005583:Fourier Analysis; D017709:Fractals; D008027:Light; D000432:Methanol / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000737:chemistry; D017433:Protein Structure, Secondary; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods; D013818:Thermogravimetry; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D010455:Peptides; C017645:polyaspartate; D005349:Fibroins; D002122:Calcium Chloride; D000432:Methanol