title: [Study on composing mechanism by effect of disassembled compositions of Yiqi Huoxue decoction on platelet aggregation of health adults].
abstract: OBJECTIVE
  To investigate and explain the composing mechanism by observing enhancement property of disassembled compositions of Yiqi Huoxue decoction (YQHXD) on platelet aggregation of health adults.
  
  
  METHODS
  Venous whole blood was obtained from healthy human subjects and anti-coagulated, and then centrifuged to produce platelet-rich plasma (PRP) and platelet-poor plasma (PPP). Disassembled compositions of YQHXD and sodium chloride were placed in different translucent tubes containing PRP and PPP, calculated the maximum percents of platelet aggregation stimulated by platelet agonists: adenosine diphosphate (ADP), platelet activating factor (PAF) and arachidonic acid (AA).
  
  
  RESULTS
  The maximum percents of platelet aggregation induced by ADP and PAF were largely improved by YQHXD, both astragalus membranaceus and Angelica Scinesis played the most important roles in the decoction, the herbs of Yiqi and Huoxue must work together to enhance platelet aggregation.
  
  
  CONCLUSIONS
  In vitro study, YQHXD can markedly improve platelet aggregation of health adults, but the exact mechanism should be proved by further experiments.
authors.affiliation: Union Medical Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: You
authors.initials: Y
authors.lastname: Qin
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Lu
authors.forename: Lan
authors.initials: L
authors.lastname: Gao
authors.forename: La-Mei
authors.initials: LM
authors.lastname: Pan
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D029971:Angelica sinensis / Q000737:chemistry; D016718:Arachidonic Acid / Q000494:pharmacology; D027885:Astragalus propinquus / Q000737:chemistry; D004338:Drug Combinations; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010946:Plants, Medicinal / Q000737:chemistry*; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D055815:Young Adult
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate