title: [Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence quantitative PCR].
abstract: OBJECTIVE
  To explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).
  
  
  METHODS
  The total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.
  
  
  RESULTS
  CCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).
  
  
  CONCLUSIONS
  CCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.
authors.affiliation: Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhao-Xia
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Cao
authors.forename: Qiong
authors.initials: Q
authors.lastname: Huang
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Yang
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D002648:Child; D056745:Cyclin C; D016213:Cyclins / Q000235:genetics*; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*
chemical_list: C503674:CCNC protein, human; D056745:Cyclin C; D016213:Cyclins