title: Plasma esterified F2-isoprostanes and oxidative stress in newborns: role of nonprotein-bound iron.
abstract: Nonprotein-bound iron (NPBI) and F2-isoprostanes, reliable markers of oxidative stress, are increased in plasma of newborns and inversely correlated to the gestational age. Because NPBI represents a pro-oxidant stimulus in plasma, we test the hypothesis that the entity of lipid peroxidation is related with NPBI concentrations. Plasma levels of free, esterified, and total F2-isoprostanes were investigated in relation to NPBI levels in 59 newborns and 16 healthy adults. The pro-oxidant role of iron was ascertained in vitro, by measuring all the forms of F2-isoprostanes after incubation with ammonium iron sulfate. Significant positive correlations were found between NPBI and total as well as esterified F2-isoprostanes in plasma of the newborns. The addition of ammonium iron sulfate induced a marked increase in all the forms of F2-isoprostanes after 2 hours of incubation. The higher NPBI concentration, the higher F2-isoprostanes levels. An increase NPBI dose dependent in total F2-isoprostanes formation was observed in dialyzed low density lipoprotein from adult plasma. The results clearly show that once NPBI is generated, whatever its source, it is capable of inducing oxidative stress. NPBI-induced oxidative stress may contribute to the morbidity in preterm infants that are particularly susceptible to free radical damage.
authors.affiliation: Departments of Pathophysiology, Experimental Medicine and Public Health, University of Siena, 53100 Siena, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Signorini
authors.forename: Serafina
authors.initials: S
authors.lastname: Perrone
authors.forename: Cristiana
authors.initials: C
authors.lastname: Sgherri
authors.forename: Lucia
authors.initials: L
authors.lastname: Ciccoli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Buonocore
authors.forename: Silvia
authors.initials: S
authors.lastname: Leoncini
authors.forename: Viviana
authors.initials: V
authors.lastname: Rossi
authors.forename: Daniela
authors.initials: D
authors.lastname: Vecchio
authors.forename: Mario
authors.initials: M
authors.lastname: Comporti
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D004951:Esterification; D028441:F2-Isoprostanes / Q000097:blood*; D005260:Female; D005296:Ferrous Compounds / Q000737:chemistry; D005865:Gestational Age; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D007501:Iron / Q000097:blood*; D007700:Kinetics; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D018384:Oxidative Stress; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D008077:Lipoproteins, LDL; D000644:Quaternary Ammonium Compounds; C038178:ammonium ferrous sulfate; D007501:Iron