[Study on composing mechanism by effect of disassembled compositions of Yiqi Huoxue decoction on platelet aggregation of health adults].
OBJECTIVE
To investigate and explain the composing mechanism by observing enhancement property of disassembled compositions of Yiqi Huoxue decoction (YQHXD) on platelet aggregation of health adults.


METHODS
Venous whole blood was obtained from healthy human subjects and anti-coagulated, and then centrifuged to produce platelet-rich plasma (PRP) and platelet-poor plasma (PPP). Disassembled compositions of YQHXD and sodium chloride were placed in different translucent tubes containing PRP and PPP, calculated the maximum percents of platelet aggregation stimulated by platelet agonists: adenosine diphosphate (ADP), platelet activating factor (PAF) and arachidonic acid (AA).


RESULTS
The maximum percents of platelet aggregation induced by ADP and PAF were largely improved by YQHXD, both astragalus membranaceus and Angelica Scinesis played the most important roles in the decoction, the herbs of Yiqi and Huoxue must work together to enhance platelet aggregation.


CONCLUSIONS
In vitro study, YQHXD can markedly improve platelet aggregation of health adults, but the exact mechanism should be proved by further experiments.
Shi
Wei
W
Union Medical Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
Shen
Lin
L
Qin
You
Y
Lu
Fu-Rong
FR
Gao
Lan
L
Pan
La-Mei
LM
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D029971:Angelica sinensis / Q000737:chemistry; D016718:Arachidonic Acid / Q000494:pharmacology; D027885:Astragalus propinquus / Q000737:chemistry; D004338:Drug Combinations; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010946:Plants, Medicinal / Q000737:chemistry*; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D055815:Young Adult
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate