[Critical collapse pressure of gas vesicles in six strains of cyanobacteria].
Two methods including filtration (vacuity is 0.02 MPa) and centrifugation ( < 500 r/min) were applied to concentrate algal biomass, and capillary compression tube and the concentrated algae suspensions, critical pressure distribution of gas vesicles in six cyanobacteria were also investigated. The results showed that unicellular Microcystis aeruginosa cells couldn't be concentrated effectively by filtration or centrifugation, but colony of Microcystis wesenbergii and Microcystis flosaquae, and filament of Planktothrix mougeotii could be concentrated by centrifugation and filtration respectively. Both filtration and centrifugation had a negligibly impact on gas vesicles content of cells, and the loss of gas vesicles was lower than 7%. The mean critical pressure values of five strains Microcystis were very close, which ranged from 0.64 to 0.67 MPa, and the mean critical pressure of P. mougeotii was 0.715 MPa. All of the six cyanobacteria were isolated from three shallow lakes, and their mean critical pressure values were lower than those cyanobacteria that inhabit in deep lakes or reservoirs. At the same condition of illumination density and temperature, turgor pressures of two unicellular Microcystis were higher than those of colonial Microcystis.
Chu
Zhao-sheng
ZS
State Environmental Protection Key Laboratory for Lake Pollution Control, Research Center of Lake Eco-Environments, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. chuzs@craes.org.cn
Yang
Bo
B
Jin
Xiang-can
XC
Yan
Feng
F
Zheng
Shuo-fang
SF
Pang
Yan
Y
Zeng
Qing-ru
QR
D018533:Biomass; D000458:Cyanobacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D046931:Microcystis / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D011312:Pressure; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; C038524:gas vesicle protein