Cloning of miniature pig HIF-1alpha and its responses to immunosuppressive agents.
Miniature pigs are worth notice as candidate donors for xenotransplantation. However, donor organs are inevitably subjected to hypoxia, which causes vascular endothelial dysfunction. In this respect, hypoxia-inducible factor 1alpha (HIF-1alpha), the key factor for hypoxic adaptation, should be expressed in grafts. However, some immunosuppressive drugs have been reported to suppress HIF-1alpha in rat cells. Here, we first identified the cDNA and protein structures of miniature pig HIF-1alpha, and next investigated the effects of cyclosporine and FK506 on HIF-1alpha expression in endothelial cells of miniature pig. Thus, we conclude that FK506, rather than cyclosporine, may be recommended for xenotransplantation using miniature pig organs.
Kim
Kyung-Eun
KE
Department of Pharmacology, Seoul National University, College of Medicine, Seoul, Korea.
Jung
Yu-Jung
YJ
Li
Shan Hua
SH
Chun
Yang-Sook
YS
Ahn
Curie
C
Park
Jong-Wan
JW
D000595:Amino Acid Sequence; D000693:Anaerobiosis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D003001:Cloning, Molecular; D016572:Cyclosporine / Q000494:pharmacology; D018076:DNA, Complementary; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000187:drug effects; D011487:Protein Conformation; D013552:Swine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013556:Swine, Miniature / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016559:Tacrolimus / Q000494:pharmacology*; D014183:Transplantation, Heterologous; D000099162:Endothelial PAS Domain-Containing Protein 1
D051792:Basic Helix-Loop-Helix Proteins; D016572:Cyclosporine; D018076:DNA, Complementary; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007166:Immunosuppressive Agents; D016559:Tacrolimus; D000099162:Endothelial PAS Domain-Containing Protein 1