title: Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide.
abstract: Recently, new DNA extraction techniques (using ethidium monoazide and propidium monoazide) have been developed to discriminate between alive and dead bacterial cells. Nevertheless, for complex environmental samples, no data are available yet. In the present study, these new methods were applied to anaerobic-fermentor sludge and the results were compared to a conventional microbiological approach.
authors.affiliation: Institute of Microbiology, University of Innsbruck, Technikerstr. 25, A-6020 Innsbruck, Austria. Andreas.Wagner@uibk.ac.at
authors.forename: Andreas O
authors.initials: AO
authors.lastname: Wagner
authors.forename: Cornelia
authors.initials: C
authors.lastname: Malin
authors.forename: Brigitte A
authors.initials: BA
authors.lastname: Knapp
authors.forename: Paul
authors.initials: P
authors.lastname: Illmer
mesh_terms: D001386:Azides / Q000378:metabolism*; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism*; D004783:Environmental Microbiology; D008967:Molecular Biology / Q000379:methods*; D011419:Propidium / Q000378:metabolism*
chemical_list: D001386:Azides; D011419:Propidium; C014535:8-azidoethidium; D004247:DNA