Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Streptomyces sp. K04-0144. I. Taxonomy, fermentation, isolation and structural elucidation.
Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, was isolated from the culture broth of Streptomyces sp. K04-0144 by Diaion HP-20 and ODS column chromatographies and preparative HPLC. The structure of cyslabdan was elucidated by spectroscopic analyses including NMR. The compound has a labdane-type diterpene skeleton connecting with an N-acetylcysteine via thioether linkage.
Fukumoto
Atsushi
A
Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan.
Kim
Yong-Pil
YP
Matsumoto
Atsuko
A
Takahashi
Yoko
Y
Shiomi
Kazuro
K
Tomoda
Hiroshi
H
Omura
Satoshi
S
D000111:Acetylcysteine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D004224:Diterpenes / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004357:Drug Synergism; D005285:Fermentation; D006801:Humans; D015378:Imipenem / Q000008:administration & dosage*; D009682:Magnetic Resonance Spectroscopy; D016106:Methicillin Resistance; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013302:Streptomyces / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D004224:Diterpenes; C529277:cyslabdan; D015378:Imipenem; D000111:Acetylcysteine