title: Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid.
abstract: OBJECTIVE
  To compare oocyte maturation, fertilization and cleavage rates, and embryonic developmental quality after culture of human immature oocytes from polycystic ovary syndrome (PCOS) patients in human tubal fluid (HTF) or tissue culture medium (TCM) 199.
  
  
  METHODS
  Prospective, randomized, controlled trial.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Thirteen women undergoing 23 in vitro maturation cycles, from whom 119 oocytes were retrieved.
  
  
  METHODS
  Cumulus-enclosed germinal vesicle-stage oocytes matured in TCM-199-supplemented or HTF-supplemented media.
  
  
  METHODS
  Oocyte maturation and fertilization rates, embryonic developmental quality.
  
  
  RESULTS
  Significant differences were observed between TCM 199 and HTF regarding maturation rate (82% vs. 56.9%), fertilization rate (70% vs. 39.4%), and embryo quality (81.3% vs. 41.7%).
  
  
  CONCLUSIONS
  Human tubal fluid medium, although widely used for embryo fertilization and maintenance in IVF techniques, is not an appropriate medium for the maturation of oocytes obtained from PCOS patients in nonstimulated cycles.
authors.affiliation: Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. cm.araujo@uol.com.br
authors.forename: Carlos Henrique Medeiros
authors.initials: CH
authors.lastname: de Araujo
authors.forename: Daniela
authors.initials: D
authors.lastname: Nogueira
authors.forename: Maria Cristina Picinato Medeiros
authors.initials: MC
authors.lastname: de Araujo
authors.forename: Wellington de Paula
authors.initials: Wde P
authors.lastname: Martins
authors.forename: Rui Alberto
authors.initials: RA
authors.lastname: Ferriani
authors.forename: Rosana Maria
authors.initials: RM
authors.lastname: dos Reis
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques; D002970:Cleavage Stage, Ovum; D003470:Culture Media; D046149:Embryo Culture Techniques; D004624:Embryo Transfer; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D054315:Oocyte Retrieval; D009865:Oocytes; D009866:Oogenesis; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000503:physiopathology / Q000628:therapy*; D011247:Pregnancy; D011446:Prospective Studies
chemical_list: D003470:Culture Media