title: Transient infiltration of neutrophils into the thymus following whole-body X-ray irradiation in IL-10 knockout mice.
abstract: IL-10 is known to suppress the inflammatory responses in a variety of experimental models. Because we previously found that whole-body X-irradiation causes massive apoptosis in the thymus and transient infiltration of neutrophils, in this study, we examined whether or not IL-10 is involved in the regulation of neutrophil infiltration upon whole-body X-ray irradiation using IL-10 knockout mice. Although IL-10 was induced in the thymus on whole-body X-ray irradiation, apoptosis of thymocytes, neutrophil infiltration, and MIP-2 and KC production in the thymus were not affected by an IL-10 deficiency. Coculturing of bone marrow-derived macrophages with late apoptotic cells caused MIP-2 production, which was also not affected by an IL-10 deficiency. These results suggest the uniqueness of the inflammatory response induced by whole-body X-ray irradiation, which does not seem to be regulated by IL-10.
authors.affiliation: Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Akiko
authors.initials: A
authors.lastname: Uzawa
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Nagata
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects*; D002478:Cells, Cultured; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018375:Neutrophil Activation / Q000502:physiology / Q000528:radiation effects; D009504:Neutrophils / Q000502:physiology* / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000379:methods*
chemical_list: D016753:Interleukin-10