Evaluation of protamines 1 and 2 transcript contents in spermatozoa from asthenozoospermic men.
During mammalian spermatogenesis, the chromatin structure undergoes substantial condensation. The key role in this process is played by protamines 1 and 2 (PRM1, PRM2). We attempted to compare the levels of PRM1 and PRM2 transcripts in mature spermatozoa of normospermic and asthenozoospermic men. Human ejaculates from normozoospermic (n=70) and asthenozoospermic (n=100) donors were purified by centrifugation through discontinuous Percoll density gradient. RNA was isolated from spermatozoa according to the Chomczyñski and Sacchi method, treated with DNase I, and reverse-transcribed into cDNA. Using reverse transcription and real-time quantitative polymerase chain reaction analysis, we found a reduction in the levels of PRM1 and PRM2 transcripts in spermatozoa from asthenozoospermic men, as compared to controls (P<0.001). Our findings indicate that a reduction in contents of PRM1 and PRM2 transcripts in spermatozoa may be linked with asthenozoospermia.
Kempisty
Bartosz
B
Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland.
Depa-Martynow
Magdalena
M
Lianeri
Margarita
M
Jedrzejczak
Piotr
P
Darul-Wasowicz
Agnieszka
A
Jagodzinski
Paweł P
PP
D053627:Asthenozoospermia / Q000235:genetics*; D006801:Humans; D008297:Male; D011479:Protamines / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics*
C082073:PRM1 protein, human; D011479:Protamines; D012333:RNA, Messenger; C068621:protamine 2