Fullerenol C60(OH)24 effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line.
Radiotherapy-induced toxicity is a major dose-limiting factor in anti-cancer treatment. Ionizing radiation leads to the formation of reactive oxygen and nitrogen species (ROS/RNS) that are associated with radiation-induced cell death. Investigations of biological effects of fullerenol have provided evidence for its ROS/RNS scavenger properties in vitro and radioprotective efficiency in vivo. Therefore we were interested to evaluate its radioprotective properties in vitro in the human erythroleukemia cell line. Pre-treatment of irradiated cells by fullerenol exerted statistically significant effects on cell numbers and the response of antioxidative enzymes to X-ray irradiation-induced oxidative stress in cells. Our study provides evidence that the pre-treatment with fullerenol enhanced the enzymatic activity of superoxide dismutase and glutathione peroxidase in irradiated K562 cells.
Bogdanović
Visnja
V
Institute of Oncology, Department of Experimental Oncology, Sremska, Kamenica, Serbia.
Stankov
Karmen
K
Icević
Ivana
I
Zikic
Dragan
D
Nikolić
Aleksandra
A
Solajić
Slavica
S
Djordjević
Aleksandar
A
Bogdanović
Gordana
G
D016166:Free Radical Scavengers / Q000494:pharmacology*; D037741:Fullerenes / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D016166:Free Radical Scavengers; D037741:Fullerenes; D011837:Radiation-Protective Agents; C108127:fullerenol; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase