title: Separation and determination of ketoprofen, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography.
abstract: Simple micellar electrokinetic chromatographic (MEKC) method was developed for the determination of ketoprofen as the active substance and methylparaben and propylparaben as preservatives in a semisolid pharmaceutical preparation. Separation was carried out with a fused silica capillary and UV detection at 200nm. Optimized background electrolyte was 50mM tricine buffer containing 30mM sodium dodecyl sulfate as surfactant and 15% (v/v) of methanol. Single separation took about 13min. No statistically significant differences were found when comparing the results with those of RP-HPLC method reported in literature.
authors.affiliation: Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic. jiri.safra@faf.cuni.cz
authors.forename: Jirí
authors.initials: J
authors.lastname: Safra
authors.forename: Marie
authors.initials: M
authors.lastname: Pospísilová
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007660:Ketoprofen / Q000032:analysis*; D010226:Parabens / Q000032:analysis*
chemical_list: D010226:Parabens; D007660:Ketoprofen; C015358:methylparaben; C006068:propylparaben