title: Single-well, gas-sparging tests for evaluating the in situ aerobic cometabolism of cis-1,2-dichloroethene and trichloroethene.
abstract: This study developed single-well, gas-sparging tests for assessing the feasibility of in situ aerobic cometabolism of trichloroethene (TCE) and cis-1,2-dichloroethene (cis-DCE) using propane and methane as growth substrates. Tests were performed in groundwater contaminated with TCE (100-400 microg l(-1)) and cis-DCE (20-60 microg l(-1)). A series of gas-sparging tests was performed by first sparging ("bubbling") gas mixtures in a well fitted with a "straddle" packer and then periodically sampling groundwater from the same well to develop concentration profiles and to estimate transformation rate coefficients. Evidence that gas-sparging of propane (or methane) and oxygen had stimulated organisms expressing a propane (or methane) monooxygenase enzyme system and the capability to transform TCE and cis-DCE included: (1) the transformation of sparged ethylene and propylene to their corresponding cometabolic by-products, ethylene oxide and propylene oxide, (2) the transformation of both cis-DCE and TCE in the propane-sparged well, (3) the transformation of cis-DCE in the methane-sparged well, and (4) the inhibition of ethylene and propylene transformations in the presence of acetylene, a known monooxygenase inactivator. At a well sparged with propane, first-order rate coefficients for propane utilization and ethylene and propylene transformation were similar, ranging from 0.007 to 0.010 h(-1). At the well sparged with methane, the propylene first-order transformation rate coefficient was 0.028 h(-1), a factor of 1.8 and 1.6 greater than methane and ethylene, respectively. The results demonstrated that gas-sparging tests are a rapid, low-cost means of assessing the potential for the in situ aerobic cometabolism of cis-DCE and TCE.
authors.affiliation: Department of Environmental Engineering, Korea University, Jochiwon-eub, Yeongi-gun, Choong Nam 339-800, Republic of Korea. kimyo@korea.ac.kr <kimyo@korea.ac.kr>
authors.forename: Young
authors.initials: Y
authors.lastname: Kim
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Istok
authors.forename: Lewis
authors.initials: L
authors.lastname: Semprini
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000378:metabolism; D004000:Dichloroethylenes / Q000378:metabolism*; D005240:Feasibility Studies; D005740:Gases; D014241:Trichloroethylene / Q000378:metabolism*
chemical_list: D004000:Dichloroethylenes; D005740:Gases; D014241:Trichloroethylene; C035384:1,2-dichloroethylene