Induction of synapse associated protein 102 expression in cyclosporin A-stimulated hair growth.
Cyclosporin A (CsA) has been used as a potent immunosuppressive agent for inhibiting the graft rejection after organ transplantation. However, CsA provokes lots of side effects including hirsutism, the phenomenon of abnormal hair growth in the body. In the present study, we investigated the hair growth stimulating effect of CsA using in vivo and in vitro test models. When topically applied on the back skin of mice, CsA induced fast telogen to anagen transition. In contrast, CsA had no effect on the growth of human hair follicle tissues cultured in vitro, indicating that it might not have the mitogenic effect on hair follicles. To identify the genes related with CsA-induced hair growth, we performed differential display RT-PCR. Among the genes obtained, the expression of synapse associated protein 102 (SAP102) was verified using competitive RT-PCR. The result showed that the expression of SAP102 was significantly induced by CsA treatment in the back skin of C57BL/6 mice. However, the increase of SAP102 mRNA was also seen in spontaneous anagen mice, suggesting that induction of SAP102 is one event of the anagen hair growth response regardless of how the growth state was induced. SAP102 was not expressed in cultured human hair outer root sheath and dermal papilla cells. Immunohistochemistry analysis showed that CsA induced the expression of SAP102 in perifollicular region of mouse anagen hair. Together, these results suggest that SAP102 is one of hair-cycle-dependent genes, whose expression is related with the anagen progression.
Kim
Chang Deok
CD
Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea.
Lee
Min-Ho
MH
Sohn
Kyung-Cheol
KC
Kim
Jin-Man
JM
Li
Sheng Jin
SJ
Rang
Moon-Jeong
MJ
Roh
Seok-Seon
SS
Oh
Young-Seon
YS
Yoon
Tae-Jin
TJ
Im
Myung
M
Seo
Young-Joon
YJ
Lee
Jeung-Hoon
JH
Park
Jang-Kyu
JK
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D016572:Cyclosporine / Q000008:administration & dosage / Q000494:pharmacology*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D051528:Guanylate Kinases; D006197:Hair / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers; D008565:Membrane Proteins; D009479:Neuropeptides; D016572:Cyclosporine; C491075:Dlgh3 protein, mouse; D051528:Guanylate Kinases