title: Toward a global database for the molecular typing of Saccharomyces cerevisiae strains.
abstract: Most of the yeast strains used in fermented beverages and foods are classified as Saccharomyces cerevisiae. However, different strains are suitable for different fermentation processes. The purpose of this work is the proposal of a standardized methodology for the molecular genotyping of S. cerevisiae strains based on polymorphisms at microsatellite loci and/or single nucleotide polymorphisms (SNPs). Single nucleotide variants in the coding region of FLO8, a key regulator of flocculation and pseudohyphae formation, were analyzed in a subset of Uruguayan wine strains. Polymorphism analysis at nine microsatellite loci (selected from 33 loci tested) was performed in a collection of 120 strains, mostly wine strains, from different origins. From a total of 184 different alleles scored, 50 were exclusive alleles that could identify 29 strains. Four selected microsatellite loci are located within or near genes of putative enological interest. The Uruguayan strains are highly diverse and evenly distributed in the phylogenetic reconstructions, suggesting an evolutionary history previous to human use. The Saccharomyces cerevisiae Microsatellites and SNPs Genotyping Database is presented (www.pasteur.edu.uy/yeast). Comparison of standardized results from strains coming from different settings (industrial, clinical, environmental) will provide a reliable and growing source of information on the molecular biodiversity of S. cerevisiae strains.
authors.affiliation: Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Uruguay.
authors.forename: Sandra
authors.initials: S
authors.lastname: Jubany
authors.forename: Ivanna
authors.initials: I
authors.lastname: Tomasco
authors.forename: Inés
authors.initials: I
authors.lastname: Ponce de León
authors.forename: Karina
authors.initials: K
authors.lastname: Medina
authors.forename: Francisco
authors.initials: F
authors.lastname: Carrau
authors.forename: Nicolás
authors.initials: N
authors.lastname: Arrambide
authors.forename: Hugo
authors.initials: H
authors.lastname: Naya
authors.forename: Carina
authors.initials: C
authors.lastname: Gaggero
mesh_terms: D019992:Databases as Topic; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D016533:Mycological Typing Techniques; D009687:Nuclear Proteins / Q000235:genetics; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012441:Saccharomyces cerevisiae / Q000145:classification* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
chemical_list: C100940:FLO8 protein, S cerevisaie; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators