title: Adult exposure to diethylstilbestrol induces spermatogenic cell apoptosis in vivo through increased oxidative stress in male hamster.
abstract: Effects of diethylstilbestrol (DES), an endocrine disrupting chemical, on abnormal spermatogenesis were studied in adult hamster using daily subcutaneous injection of 0.01, 0.1 and 1.0mg/kg body weight for 1 week. Testicular weight and seminiferous tubular area gradually decreased as dosage increased to 1.0mg/kg DES. Germ cells were rarefied and showed irregular distribution in seminiferous tubules. Apoptosis was pronounced among spermatocytes and spermatids at the 1.0mg/kg dose level. Antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and total antioxide capacity (T-AOC) markedly decreased and malondialdehyde (MDA) concentration significantly increased in the testes. These results suggest that DES (1.0mg/kg) induces testicular oxidative stress and spermatogenic apoptosis in adult male hamsters to extend findings shown for prenatal and/or neonatal exposure.
authors.affiliation: Laboratory of Veterinary Anatomy, College of Animal Medicine, China Agricultural University, Beijing 100094, China.
authors.forename: Aituan
authors.initials: A
authors.lastname: Ma
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Yang
authors.forename: Zixu
authors.initials: Z
authors.lastname: Wang
authors.forename: Dazhao
authors.initials: D
authors.lastname: Shi
authors.forename: Yaoxing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006224:Cricetinae; D004054:Diethylstilbestrol / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008297:Male; D008647:Mesocricetus; D018384:Oxidative Stress / Q000187:drug effects*; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013094:Spermatozoa / Q000187:drug effects* / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000473:pathology
chemical_list: D004054:Diethylstilbestrol