title: Dipeptidyl peptidase I-dependent neutrophil recruitment modulates the inflammatory response to Sendai virus infection.
abstract: The role of innate immunity in the pathogenesis of asthma is unclear. Although increased presence of neutrophils is associated with persistent asthma and asthma exacerbations, how neutrophils participate in the pathogenesis of asthma remains controversial. In this study, we show that the absence of dipeptidyl peptidase I (DPPI), a lysosomal cysteine protease found in neutrophils, dampens the acute inflammatory response and the subsequent mucous cell metaplasia that accompanies the asthma phenotype induced by Sendai virus infection. This attenuated phenotype is accompanied by a significant decrease in the accumulation of neutrophils and the local production of CXCL2, TNF, IL-1beta, and IL-6 in the lung of infected DPPI-/- mice. Adoptive transfer of DPPI-sufficient neutrophils into DPPI-/- mice restored the levels of CXCL2 and enhanced cytokine production on day 4 postinfection and subsequent mucous cell metaplasia on day 21 postinfection. These results indicate that DPPI and neutrophils play a critical role in Sendai virus-induced asthma phenotype as a result of a DPPI-dependent neutrophil recruitment and cytokine response.
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Antonina M
authors.initials: AM
authors.lastname: Akk
authors.forename: Pamela M
authors.initials: PM
authors.lastname: Simmons
authors.forename: Happy W
authors.initials: HW
authors.lastname: Chan
authors.forename: Eugene
authors.initials: E
authors.lastname: Agapov
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Holtzman
authors.forename: Mitchell H
authors.initials: MH
authors.lastname: Grayson
authors.forename: Christine T N
authors.initials: CT
authors.lastname: Pham
mesh_terms: D000818:Animals; D001249:Asthma / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D020789:Cathepsin C / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D007249:Inflammation / Q000201:enzymology / Q000276:immunology / Q000821:virology; D008297:Male; D008679:Metaplasia / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D020556:Neutrophil Infiltration / Q000276:immunology*; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology* / Q000473:pathology*; D020545:Respiratory Mucosa / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D010253:Respirovirus Infections / Q000201:enzymology / Q000276:immunology* / Q000473:pathology*; D029082:Sendai virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology / Q000276:immunology / Q000473:pathology
chemical_list: D020789:Cathepsin C