Gene expression studies on bio-electrosprayed primary cardiac myocytes.
Cardiovascular pathology accounts for the greatest number of mortalities in the western world and thus the development of ex vivo cardiac tissue has vast potential in cardiac therapy. Bio-electrosprays (BES), a recently discovered direct cell engineering protocol, has demonstrated tremendous applicability for regenerative and therapeutic medicine. For bio-electrospraying to be carried forward as a novel method of cardiac tissue engineering, it is important that the process does not adversely affect cellular physiology. Our previous work has shown that bio-electrospraying does not induce cell death, activate intracellular stress pathways or induce DNA damage in primary cardiac myocytes. Here we show for the first time using genome-wide microarray analysis, that bio-electrospraying has no negative effects on global gene expression in cardiac myocytes. Moreover, we show that bio-electrospraying does not lead to endothelial cell activation. These data suggest that BES has minimal effect upon the physiology of cardiac myocytes and endothelial cells and thus paves the way for the development of BES in cardiac tissue engineering.
Barry
Seán P
SP
Medical Molecular Biology Unit, Institute of Child Health, University College London, London, UK.
Jayasinghe
Suwan N
SN
Pericleous
Charis
C
Hubank
Mike
M
Latchman
David S
DS
Stephanou
Anastasis
A
D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D044085:Microfluidics / Q000379:methods; D008846:Micromanipulation / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*
D020543:Proteome