Effect of borate glass composition on its conversion to hydroxyapatite and on the proliferation of MC3T3-E1 cells.
Glasses containing varying amounts of B(2)O(3) were prepared by partially or fully replacing the SiO(2) in silicate 45S5 bioactive glass with B(2)O(3). The effects of the B(2)O(3) content of the glass on its conversion to hydroxyapatite (HA) and on the proliferation of MC3T3-E1 cells were investigated in vitro. Conversion of the glasses to HA in dilute (20 mM) K(2)HPO(4) solution was monitored using weight loss and pH measurements. Proliferation of MC3T3-E1 cells was determined qualitatively by assay of cell density at the glass interface after incubation for 1 day and 3 days, and quantitatively by fluorescent measurements of total DNA in cultures incubated for 4 days. Higher B(2)O(3) content of the glass increased the conversion rate to HA, but also resulted in a greater inhibition of cell proliferation under static culture conditions. For a given mass of glass in the culture medium, the inhibition of cell proliferation was alleviated by using glasses with lower B(2)O(3) content, by incubating the cell cultures under dynamic rather than static conditions, or by partially converting the glass to HA prior to cell culture.
Brown
Roger F
RF
Department of Biological Sciences, University of Missouri, Rolla, Missouri 65409, USA.
Rahaman
Mohamed N
MN
Dwilewicz
Agatha B
AB
Huang
Wenhai
W
Day
Delbert E
DE
Li
Yadong
Y
Bal
B Sonny
BS
D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002516:Ceramics; D003470:Culture Media / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D010710:Phosphates / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
D001672:Biocompatible Materials; D001896:Boron Compounds; D003470:Culture Media; D006882:Hydroxyapatites; D010710:Phosphates; C425330:bioactive glass 45S5; C042168:boron oxide; D012822:Silicon Dioxide