Adsorbed serum albumin is permissive to macrophage attachment to perfluorocarbon polymer surfaces in culture.
Monocyte/macrophage adhesion to biomaterials, correlated with foreign body response, occurs through protein-mediated surface interactions. Albumin-selective perfluorocarbon (FC) biomaterials are generally poorly cell-conducive because of insufficient receptor-mediated surface interactions, but macrophages bind to albumin-coated substrates and also preferentially to highly hydrophobic fluorinated surfaces. Bone marrow macrophages (BMMO) and IC-21, RAW 264.7, and J774A.1 monocyte/macrophage cells were cultured on FC surfaces. Protein deposition onto two distinct FC surfaces from complex and single-component solutions was tracked using fluorescence and time-of-flight secondary ion mass spectrometry (ToF-SIMS) methods. Cell adhesion and growth on protein pretreated substrates were compared by light microscopy. Flow cytometry and integrin-directed antibody receptor blocking were used to assess integrins critical for monocyte/macrophage adhesion in vitro. Albumin predominantly adsorbs onto both FC surfaces from 10% serum. In cultures preadsorbed with albumin or serum-dilutions, BMMO responded similar to IC-21 at early time points. Compared with Teflon AF, plasma-polymerized FC was less permissive to extended cell proliferation. The beta(2) integrins play major roles in macrophage adhesion to FC surfaces: antibody blocking significantly disrupted cell adhesion. Albumin-mediated cell adhesion mechanisms to FC surfaces could not be clarified. Primary BMMO and secondary IC-21 macrophages behave similarly on FC surfaces, regardless of preadsorbed protein biasing, with respect to adhesion, cell morphology, motility, and proliferation.
Godek
M L
ML
Cell and Molecular Biology Program, Colorado State University, Fort Collins, Colorado 80523, USA.
Michel
R
R
Chamberlain
L M
LM
Castner
D G
DG
Grainger
D W
DW
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology*; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D005353:Fibronectins / Q000378:metabolism; D005466:Fluorocarbons / Q000737:chemistry / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D011138:Polytetrafluoroethylene / Q000737:chemistry / Q000378:metabolism; D025341:Principal Component Analysis; D012709:Serum Albumin / Q000378:metabolism*
D001672:Biocompatible Materials; D005353:Fibronectins; D005466:Fluorocarbons; D016023:Integrins; D011108:Polymers; D012709:Serum Albumin; D011138:Polytetrafluoroethylene