Microscale HPLC enables a new paradigm for commercialization of complex chiral stationary phases.
The small column size (0.3 mm i.d. x 15 cm) used in microscale HPLC contains only a small fraction (<1%) of the chromatographic packing material of a typical analytical HPLC column. Consequently, chromatographic stationary phases that are prohibitively expensive in conventional HPLC, owing either to synthetic complexity or costly starting materials, may become commercially viable in the microscale format. To illustrate this point, a previously described, synthetically complex, crown ether chiral stationary phase was prepared and evaluated in the microscale format, showing excellent separation of the enantiomers of underivatized amine analytes.
Welch
Christopher J
CJ
Separation and Purification Center of Excellence, Merck & Co, Inc., Rahway, New Jersey 07065, USA. christopher_welch@merck.com
Hyun
Myung Ho
MH
Kubota
Takateru
T
Schafer
Wes
W
Bernardoni
Frank
F
Choi
Hee Jung
HJ
Wu
Naijun
N
Gong
Xiaoyi
X
Lipshutz
Bruce
B
D000588:Amines / Q000737:chemistry / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D043844:Crown Ethers / Q000138:chemical synthesis / Q000737:chemistry; D007202:Indicators and Reagents; D008832:Microchemistry / Q000379:methods; D013237:Stereoisomerism
D000588:Amines; D043844:Crown Ethers; D007202:Indicators and Reagents