Effects of adjuvant chemotherapy on bone marrow mesenchymal stem cells of colorectal cancer patients.
BACKGROUND
Chemotherapy damages the bone marrow and that is one of the most important problems in the treatment of malignancies, particularly colorectal cancer. The aim of the present study was to assess the effects of surgical adjuvant chemotherapy for CRC patients on human MSCs using an in vitro culture system.


METHODS
The bone marrows of 43 CRC patients were harvested for separation and culture of MSC at pre- and post-chemotherapy. The number of colonies forming unit-fibroblast (CFU-F) was counted. The adhesive function of MSC was assayed and the growth of colony-forming unit-mixed hematopoietic cell (CFU-Mix) on the MSC layer was observed. The concentration of IL-6, SCF and FLT-T3 proteins in the MSC culture supernatant were also detected by ELISA assay.


RESULTS
In the CRC patients with chemotherapy, we have demonstrated that the CFU-F exhibit significantly decreased. We also showed that the adhesive rate of bone marrow mesenchymal stem cell (BMSC) was significantly decreased. The growth of CFU-Mix on the MSC layer was inhibited. Most importantly, decreased CFU-F and the adhesive rate of BMSC were correlated significantly with decreased interleukins and stem-cell factor (IL-6, SCF and FLT-3L) expressions in the CRC patients after chemotherapy.


CONCLUSIONS
Our results suggest that MSCs of CRC patients can be damaged by chemotherapy. Our data also indicates that the decreased expression of haematogenesis factors may play an important role in the pathogenesis. In the future, the MSC refused may have a potential clinical application in chemotherapeutically treated patients.
Cao
J
J
Department of Gastrointestinal Surgery, Affiliated Guangzhou First Municipal People's Hospital, Guangzhou Medical College, 1# Pan Fu Road, Guangzhou, Guangdong 510180, China. czhongt@126.com
Tan
M H
MH
Yang
P
P
Li
W L
WL
Xia
J
J
Du
H
H
Tang
W B
WB
Wang
H
H
Chen
X W
XW
Xiao
H Q
HQ
D000368:Aged; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape; D002478:Cells, Cultured; D017024:Chemotherapy, Adjuvant / Q000009:adverse effects*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000601:surgery; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000473:pathology; D008875:Middle Aged; D014410:Tumor Stem Cell Assay
D016207:Cytokines