title: First report of Cryptosporidium deer-like genotype in Kenyan cattle.
abstract: The objective of the study was to identify Cryptosporidium genotypes from feces collected from urban and peri-urban dairy cattle in Nairobi, Kenya, in order to determine their zoonotic potential. DNA was extracted from 34 samples that were diagnosed positive by the modified Ziehl-Neelsen technique. Two Cryptosporidium isolates examined at the 18S rRNA locus were identified as the deer-like genotype by DNA sequencing. As public health officials are facing the difficult decision whether to allow urban livestock production because of its economic benefits and a livelihood asset to the urban communities, or to ban it for its public health risks, the finding of non-zoonotic genotypes in a smallholder dairy system has significant public health as well as economic implications that merit further investigation.
authors.affiliation: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Barbara
authors.initials: B
authors.lastname: Szonyi
authors.forename: Erastus K
authors.initials: EK
authors.lastname: Kang'ethe
authors.forename: Cecilia Kathure
authors.initials: CK
authors.lastname: Mbae
authors.forename: Erastus M
authors.initials: EM
authors.lastname: Kakundi
authors.forename: Stanley K
authors.initials: SK
authors.lastname: Kamwati
authors.forename: Hussni O
authors.initials: HO
authors.lastname: Mohammed
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D002947:Cities; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics; D016054:DNA, Protozoan / Q000145:classification / Q000235:genetics; D003612:Dairying; D003670:Deer / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D005838:Genotype; D007630:Kenya / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan