title: The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic.
abstract: We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1.
authors.affiliation: Department of Molecular Biology, Sejong University, 98 Kunja-Dong, Kwangjin-Gu, Seoul, Republic of Korea.
authors.forename: Young Geun
authors.initials: YG
authors.lastname: Mok
authors.forename: Byoung Doo
authors.initials: BD
authors.lastname: Lee
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Chang Eun
authors.initials: CE
authors.lastname: Lee
authors.forename: Dong Gwan
authors.initials: DG
authors.lastname: Kim
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee
authors.forename: Jaekyung
authors.initials: J
authors.lastname: Shim
authors.forename: Yuling
authors.initials: Y
authors.lastname: Meng
authors.forename: Barry P
authors.initials: BP
authors.lastname: Rosen
authors.forename: Jong Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Hyoung Sun
authors.initials: HS
authors.lastname: Shin
authors.forename: Seong-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: June Seung
authors.initials: JS
authors.lastname: Lee
authors.forename: Seongbin
authors.initials: S
authors.lastname: Hwang
mesh_terms: D000595:Amino Acid Sequence; D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D018053:Arsenites / Q000494:pharmacology*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics*; D017343:Genes, Plant; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
chemical_list: C109281:ACR3 protein, S cerevisiae; D018053:Arsenites; D050976:Basic-Leucine Zipper Transcription Factors; C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D012097:Repressor Proteins; C077832:SKO1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C015001:arsenite; D001151:Arsenic