title: YjgF is required for isoleucine biosynthesis when Salmonella enterica is grown on pyruvate medium.
abstract: The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. In Salmonella enterica, a deletion of yjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium, yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Dr., Madison, WI 53706, USA.
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Christopherson
authors.forename: G E
authors.initials: GE
authors.lastname: Schmitz
authors.forename: Diana M
authors.initials: DM
authors.lastname: Downs
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways; D017353:Gene Deletion; D005947:Glucose / Q000378:metabolism; D007532:Isoleucine / Q000096:biosynthesis*; D008954:Models, Biological; D019289:Pyruvic Acid / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013489:Suppression, Genetic; D013913:Threonine Dehydratase / Q000378:metabolism*; D000637:Transaminases / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C116617:YjgF protein, Bacteria; D007532:Isoleucine; D012694:Serine; D019289:Pyruvic Acid; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase; D013913:Threonine Dehydratase; D005947:Glucose