title: Dietary plasma proteins modulate the immune response of diffuse gut-associated lymphoid tissue in rats challenged with Staphylococcus aureus enterotoxin B.
abstract: We have previously shown that plasma protein supplementation prevents the activation of lymphocyte populations of Peyer's patches and mesenteric lymph nodes, which is known as organized gut-associated lymphoid tissue (GALT). Here, we examined the effects of spray-dried plasma proteins (SDAP) and Ig concentrate (IgC) supplements on lamina propria and intraepithelial lymphocytes (diffuse GALT) in a model of mild intestinal inflammation induced by the intraperitoneal administration of Staphylococcus aureus enterotoxin B (SEB). Wistar-Lewis rats were fed diets supplemented with SDAP (8% wt:wt), IgC (1.5% wt:wt), or bovine milk proteins (control diet) from weaning (d 21) to d 34 after birth. On d 30 and 33, rats were given SEB (0.5 mg/kg body weight) or PBS (control). Experimental groups were designated control, SEB, SEB-SDAP, and SEB-IgC. Lymphocyte populations were analyzed by immunohistochemistry. In lamina propria, SEB increased the cytotoxic lymphocyte populations of T-gammadelta cells (38%; P < 0.001) and natural killer cells (59%; P < 0.05) and the number of activated T lymphocytes (148%; P < 0.001). Both SDAP and IgC decreased the effects of SEB on these lymphocyte subsets (P < 0.05). In the epithelium, SEB induced a 117% increase in intraepithelial-activated lymphocytes that was reduced by SDAP supplementation (P < 0.01). The effects of plasma supplements on intestinal lymphocyte populations suggest that oral plasma proteins can modulate the degree of activation of diffuse GALT.
authors.affiliation: Grup de Fisiologia i Nutrició Experimental, Departament de Fisiologia, Facultat de Farmàcia, Institut de Recerca en Nutrició i Seguretat Alimentària, Universitat de Barcelona, Barcelona 08028, Spain. anna.perez@ub.edu
authors.forename: Anna
authors.initials: A
authors.lastname: Pérez-Bosque
authors.forename: Lluïsa
authors.initials: L
authors.lastname: Miró
authors.forename: Javier
authors.initials: J
authors.lastname: Polo
authors.forename: Louis
authors.initials: L
authors.lastname: Russell
authors.forename: Joy
authors.initials: J
authors.lastname: Campbell
authors.forename: Eric
authors.initials: E
authors.lastname: Weaver
authors.forename: Joe
authors.initials: J
authors.lastname: Crenshaw
authors.forename: Miquel
authors.initials: M
authors.lastname: Moretó
mesh_terms: D000818:Animals; D004044:Dietary Proteins / Q000494:pharmacology*; D004768:Enterotoxins / Q000633:toxicity*; D007583:Jejunum / Q000166:cytology / Q000473:pathology; D008221:Lymphoid Tissue / Q000187:drug effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013211:Staphylococcus aureus; D013601:T-Lymphocytes / Q000187:drug effects
chemical_list: D004044:Dietary Proteins; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal