Different roles of odontoblasts and fibroblasts in immunity.
Odontoblasts and fibroblasts are suspected to influence the innate immune response triggered in the dental pulp by micro-organisms that progressively invade the human tooth during the caries process. To determine whether they differ in their responses to oral pathogens, we performed a systematic comparative analysis of odontoblast-like cell and pulp fibroblast responses to TLR2-, TLR3-, and TLR4-specific agonists (lipoteichoic acid [LTA], double-stranded RNA, and lipopolysaccharide [LPS], respectively). Cells responded to these agonists by differential up-regulation of chemokine gene expression. CXCL2 and CXCL10 were thus increased by LTA only in odontoblast-like cells, while LPS increased CCL7, CCL26, and CXCL11 only in fibroblasts. Supernatants of stimulated cultures increased migration of immature dendritic cells compared with controls, odontoblast-like cells being more potent attractants than fibroblasts. Analysis of these data suggests that odontoblasts and pulp fibroblasts differ in their innate immune responses to oral micro-organisms that invade the pulp tissue.
Staquet
M-J
MJ
Institut de Génomique Fonctionnelle de Lyon, Université Lyon 1, CNRS, INRA, Ecole Normale Supérieure de Lyon, INSERM ERI16, Faculté d'Odontologie, Lyon, France. marie-jeanne.staquet@recherche.univ-lyon1.fr
Durand
S H
SH
Colomb
E
E
Roméas
A
A
Vincent
C
C
Bleicher
F
F
Lebecque
S
S
Farges
J-C
JC
D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D054413:Chemokine CCL11 / Q000032:analysis; D000075906:Chemokine CCL26; D054410:Chemokine CCL7 / Q000032:analysis; D054357:Chemokine CXCL10 / Q000032:analysis; D054426:Chemokine CXCL2 / Q000032:analysis; D019742:Chemokines, CC / Q000032:analysis; D003713:Dendritic Cells / Q000276:immunology; D003782:Dental Pulp / Q000187:drug effects / Q000276:immunology*; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009804:Odontoblasts / Q000187:drug effects / Q000276:immunology*; D012330:RNA, Double-Stranded / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000276:immunology; D013682:Teichoic Acids / Q000494:pharmacology; D051195:Toll-Like Receptor 2 / Q000819:agonists; D051196:Toll-Like Receptor 3 / Q000819:agonists; D051197:Toll-Like Receptor 4 / Q000819:agonists; D015854:Up-Regulation
C515456:CCL11 protein, human; C120429:CCL26 protein, human; C515450:CCL7 protein, human; C114753:CXCL10 protein, human; C499706:CXCL2 protein, human; D054413:Chemokine CCL11; D000075906:Chemokine CCL26; D054410:Chemokine CCL7; D054357:Chemokine CXCL10; D054426:Chemokine CXCL2; D019742:Chemokines, CC; D008070:Lipopolysaccharides; D012330:RNA, Double-Stranded; C495342:TLR2 protein, human; C495344:TLR3 protein, human; C495345:TLR4 protein, human; D013682:Teichoic Acids; D051195:Toll-Like Receptor 2; D051196:Toll-Like Receptor 3; D051197:Toll-Like Receptor 4; C009900:lipoteichoic acid