title: 1H and 13C NMR assignments of three nitrogen containing compounds from the mangrove endophytic fungus (ZZF08).
abstract: A new natural product, named phomopsin A, 1-(meta-hydroxyphenyl)-4-hydroxy-3-isoquinolone (1), together with two known compounds cytochalasin H (2) and glucosylceramide (3), was isolated from the mangrove endophytic fungus Phomopsis sp. (ZZF08) obtained from the South China Sea coast. The structures were elucidated by 1D and 2D NMR experiments including COSY, HMQC, and HMBC. According to NMR and single-crystal X-ray diffraction, it was found that some assignments about (1)H and (13)C NMR data for cytochalasin H (2) were probably uncorrected in the previous reports. In our cytotoxicity assays, compound 1 showed moderate cytotoxicity toward KB cells with IC(50) at 28.0 microg ml(-1) and KBv200 cells with IC(50) at 16.8 microg ml(-1), and compound 2 exhibited strong cytotoxicity toward KB cells and KBv200 cells with IC(50) less than 1.25 microg ml(-1).
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275, PR China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Tao
authors.forename: Xianjian
authors.initials: X
authors.lastname: Zeng
authors.forename: Chengbo
authors.initials: C
authors.lastname: Mou
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Cai
authors.forename: Zhigang
authors.initials: Z
authors.lastname: She
authors.forename: Shining
authors.initials: S
authors.lastname: Zhou
authors.forename: Yongcheng
authors.initials: Y
authors.lastname: Lin
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D002250:Carbon Radioisotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D017672:Nitrogen Compounds / Q000032:analysis / Q000737:chemistry*; D011522:Protons; D043982:Rhizophoraceae / Q000382:microbiology*
chemical_list: D002250:Carbon Radioisotopes; D017672:Nitrogen Compounds; D011522:Protons