[Effect of TRAIL gene in human squamous cell carcinoma cell line induced by adenovirus].
OBJECTIVE
To study the apoptotic effect on the squamous cell carcinoma cell line TCa83 induced by recombined adenovirus vector containing TRAIL gene and CMV promoter.


METHODS
The TCa83 cell line was firstly infected with different titre of AdCMV-EGFP containing enhanced green fluorescence protein gene (EGFP) as control, and investigated the transducing rate through fluorescence to obtain the definite titre. Then TCa83 cell line was infected with AdCMV-TRAIL in proper titre, and TRAIL gene was detected by means of RT-PCR. After TCa83 cell line was infected with AdCMV-TRAIL and AdCMV-EGFP at day 1, 3, 5, 7, the activity of TCa83 cell line were evaluated by MIT and the apoptosis were detected by flow cytometer.


RESULTS
Proper titre was of 1,000 particles/cell, and TCa83 cell line could be infected 100% in this titre. TRAIL gene was detected by RT-PCR after infected with AdCMV-TRAIL. The activity of TCa83 decreased in both groups, but the AdCMV-TRAIL group decreased more sharply than AdCMV-EGFP group (P < 0.001). Both AdCMV-TRAIL and AdCMV-EGFP could lead to apoptosis of TCa83 cells, but the AdCMV-TRAIL, function stronger than AdCMV-EGFP. Especially there was remarkable statistic difference between two groups (P < 0.0001).


CONCLUSIONS
AdCMV-TRAIL could effectively decrease the activity of TCa83 cell line and induce apoptosis.
Zang
Guang-Xiang
GX
Dept of Oral Pathology, School of Stomatology, Jilin University, Changchun 130041, China.
Mu
Ya-Bing
YB
Sun
Hong-Chen
HC
Liu
Jin-Zhong
JZ
Zhang
Ze-Bing
ZB
Ke
Xiao-Liang
XL
Miao
Lei-Ying
LY
D000256:Adenoviridae; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D011401:Promoter Regions, Genetic
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins