The -590 C/T and 8375 A/G interleukin-4 polymorphisms are not associated with Kawasaki disease in Taiwanese children.
Although some previous studies have reported that genetic and immunologic factors play important roles in the pathogenesis of Kawasaki disease (KD), the etiologic factors of this enigmatical pediatric disease are still poorly understood. This study aims to investigate whether polymorphisms of the interleukin-4 gene (IL-4; -590 C/T in the promoter region and 8375 A/G in intron 3) are associated with KD and the development of coronary artery lesions (CALs) in Taiwanese children. Genomic DNA was extracted from whole-blood samples from 150 children with KD and 472 ethnically matched healthy control subjects. The IL-4 -590 C/T and 8375 A/G single nucleotide polymorphisms (SNPs) were genotyped by a real-time polymerase chain reaction system with the Pre-Developed TaqMan Allelic Discrimination Assay. No significant associations between IL-4 SNPs and susceptibility of KD with CALs were found. In addition, no evidence for associations between IL-4 SNPs and CAL development was found. These results suggest that IL-4 -590 C/T and 8375 A/G SNPs do not confer a relevant role in the susceptibility or CAL development of KD in Taiwanese children.
Huang
Fu-Yuan
FY
Department of Pediatrics, Mackay Memorial Hospital, Taipei, Taiwan.
Chang
Tzu-Yang
TY
Chen
Ming-Ren
MR
Lee
Hung-Chang
HC
Chiu
Nan-Chang
NC
Chi
Hsin
H
Hsu
Chyong-Hsin
CH
Lin
Shuan-Pei
SP
Liu
Hsin-Fu
HF
Chen
Wei-Fang
WF
Chu
Chen-Chung
CC
Lin
Marie
M
Lee
Yann-Jinn
YJ
D002648:Child; D002675:Child, Preschool; D003324:Coronary Artery Disease / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D009080:Mucocutaneous Lymph Node Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013624:Taiwan
D015847:Interleukin-4