In vitro effects of bovine lactoferrin on autoaggregation ability and surface hydrophobicity of bifidobacteria.
Changes in autoaggregation ability and surface hydrophobicity of bifidobacteria with addition of bovine lactoferrin in liquid media were investigated. Lactoferrin addition caused loss of autoaggregation ability, disappearance of microscopic clusters and produced consistent turbidity in the cultured medium compared with control. Similar outcomes with addition of bovine lactoferrin hydrolysates (pepsin), bovine transferrin or ovotransferrin suggested that the effect is not lactoferrin-specific. On the other hand, addition of proteins, except bovine transferrin, did not alter surface hydrophobicity. These results indicate that one or more surface components involved in autoaggregation of bifidobacteria are proteins.
Rahman
Morshedur
M
Laboratory of Dairy Food Science, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan. morshedur68@yahoo.com
Kim
Woan-Sub
WS
Kumura
Haruto
H
Shimazaki
Kei-ichi
K
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D001644:Bifidobacterium / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D002417:Cattle; D003470:Culture Media / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007781:Lactoferrin / Q000378:metabolism*; D008853:Microscopy; D009391:Nephelometry and Turbidimetry; D014168:Transferrin / Q000378:metabolism
D003470:Culture Media; D014168:Transferrin; D007781:Lactoferrin