title: Crystal structure of human transgelin.
abstract: Transgelin (TAGLN), also known as smooth muscle protein 22 (SM22), is a highly conserved protein found in smooth muscle tissues of adult vertebrates. Abolition of transgelin gene expression by the oncogenic Ras may be an important early event in tumor progression and a diagnostic marker for breast and colon cancer development. Transgelin contains a single calponin homology (CH) domain. However, the question of whether this single CH domain can bind actin remains open. Here we report the 2.3 A resolution crystal structure of full length human transgelin, whose main structural feature is confirmed to be a CH domain. Secondary structures of CH domains from different proteins were analyzed and conserved residues were identified that maintain similar tertiary structures.
authors.affiliation: "Tsinghua-Nankai-IBP Joint Research Group for Structural Biology", Tsinghua University, Beijing 100084, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Shentao
authors.initials: S
authors.lastname: Li
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Lou
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liao
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Meng
authors.forename: Mark
authors.initials: M
authors.lastname: Bartlam
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry*; D009124:Muscle Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin