14-3-3 proteins regulate the potassium channel KAT1 by dual modes.
KAT1 is a cloned plant potassium channel belonging to the superfamily of Shaker-like Kv channels. Previous studies have shown that 14-3-3 proteins significantly increase KAT1 current by modifying the channel open probability. Employing a 14-3-3 scavenger construct to lower the long-term availability of endogenous 14-3-3 proteins, we found that 14-3-3 proteins not only control the voltage dependency of the channel but also the number of channels in the plasma membrane.
Sottocornola
B
B
Dipartimento di Biologia and IBF-CNR, Università degli Studi di Milano, Milano, Italy.
Gazzarrini
S
S
Olivari
C
C
Romani
G
G
Valbuzzi
P
P
Thiel
G
G
Moroni
A
A
D048948:14-3-3 Proteins / Q000235:genetics / Q000502:physiology*; D000818:Animals; D004594:Electrophysiology; D005260:Female; D015640:Ion Channel Gating / Q000235:genetics / Q000502:physiology*; D008564:Membrane Potentials; D009865:Oocytes / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis
D048948:14-3-3 Proteins; D010940:Plant Proteins; D024661:Potassium Channels, Inwardly Rectifying