title: [Simultaneous resolution and determination of tyrosine, tryptophan and phenylalanine by alternating penalty trilinear decomposition algorithm coupled with 3D emission-excitation matrix fluorometry].
abstract: A new method using alternating penalty trilinear decomposition algorithm coupled with excitation-emission matrix fluorometry has been developed for simultaneous resolution and determination of tyrosine, phenylalanine and tryptophan. Their correlation coefficients were 0.9987, 0.9995 and 0.9993 respectively. The contents of tyrosine, phenylalanine and tryptophan in Hibiscus syriacus L. leaves were also be determined by this method after being extracted by ultrasonic. The coefficients of variation and the recoveries of the three amino acids were 0.84%, 0.36%, 1.59% and 101.0%-92.7%, 106.5%-93.0%, 103.0%-95.0% respectively. All these show that this is a simple, fast and cridible method.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. xj410001@yahoo.com.cn
authors.forename: Jin
authors.initials: J
authors.lastname: Xiao
authors.forename: Feng-lian
authors.initials: FL
authors.lastname: Ren
authors.forename: Ge
authors.initials: G
authors.lastname: Song
authors.forename: Lü
authors.initials: L
authors.lastname: Liao
authors.forename: Wen-feng
authors.initials: WF
authors.lastname: Yu
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
mesh_terms: D000465:Algorithms; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000737:chemistry; D010649:Phenylalanine / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014364:Tryptophan / Q000032:analysis*; D014443:Tyrosine / Q000032:analysis*
chemical_list: D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan; C010882:chlorhexidine gluconate; D002710:Chlorhexidine