title: [Refinement study of macroporous resin for Huang-Lian-Jie-Du decoction].
abstract: OBJECTIVE
  To study refinement of macroporous resin to Huang-Lian-Jie-Du decoction.
  
  
  METHODS
  The content changes of berberine, geniposide and total alkaloid in the solution which went through the macroporous resin were determined by TLC or spectrophotometry. The type of resin, absorption and desorption condition were optimized.
  
  
  RESULTS
  YWD-09D macroporous resin was suitable for refinement of Huang-Lian-Jie-Du decoction. The absorptive capacity of the resin was 1: 1.3. The concentration of solution was 0.4 g/ml, pH 4 and current velocity was 2 ml/min. The ratio of diameter and high of the resin column was 1:20. The desorption solution was 90% alcohol of 4BV.
  
  
  CONCLUSIONS
  YWD-09D macroporous resin and its selected condition are suitable for refinement of Huang-Lian-Jie-Du decoction.
authors.affiliation: Shandong Institute of TCM, Jinan 250014, China.
authors.forename: Hui-bin
authors.initials: HB
authors.lastname: Lin
authors.forename: Ning
authors.initials: N
authors.lastname: Lu
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Yang
authors.forename: Jian-qun
authors.initials: JQ
authors.lastname: Lin
authors.forename: Jian-qiang
authors.initials: JQ
authors.lastname: Lin
mesh_terms: D000327:Adsorption; D000470:Alkaloids / Q000032:analysis; D001599:Berberine / Q000032:analysis; D039323:Coptis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D032102:Phellodendron / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; D012997:Solvents; C061676:oren gedoku to; D001599:Berberine