Combinatorial selection of a single stranded DNA thioaptamer targeting TGF-beta1 protein.
A phosphorothioate single-stranded DNA aptamer (thioaptamer) targeting transforming growth factor-beta1 (TGF-beta1) was isolated by in-vitro combinatorial selection. The aptamer selection procedure was designed to modify the backbone of single-stranded DNA aptamers, where 5' of both A and C are phosphorothioates, since this provides enhanced nuclease resistance as well as higher affinity than that of a phosphate counterpart. The thioaptamer selected from a combinatorial library (5x10(14) sequences) binds to TGF-beta1 protein with an affinity of 90 nM. In this report, sequence, predicted secondary structure, and binding affinity of the selected thioaptamer (T18_1_3) are presented.
Kang
Jonghoon
J
Sealy Center for Structural Biology and Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-1157, USA.
Lee
Myung Soog
MS
Copland
John A
JA
Luxon
Bruce A
BA
Gorenstein
David G
DG
D052157:Aptamers, Nucleotide / Q000494:pharmacology*; D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D017931:DNA Primers; D004277:DNA, Single-Stranded / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D049449:Luminescence; D008969:Molecular Sequence Data; D054735:Phosphorothioate Oligonucleotides / Q000494:pharmacology*; D011485:Protein Binding; D013816:Thermodynamics; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors*
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D004277:DNA, Single-Stranded; D054735:Phosphorothioate Oligonucleotides; D053773:Transforming Growth Factor beta1