Crystal structure of human transgelin.
Transgelin (TAGLN), also known as smooth muscle protein 22 (SM22), is a highly conserved protein found in smooth muscle tissues of adult vertebrates. Abolition of transgelin gene expression by the oncogenic Ras may be an important early event in tumor progression and a diagnostic marker for breast and colon cancer development. Transgelin contains a single calponin homology (CH) domain. However, the question of whether this single CH domain can bind actin remains open. Here we report the 2.3 A resolution crystal structure of full length human transgelin, whose main structural feature is confirmed to be a CH domain. Secondary structures of CH domains from different proteins were analyzed and conserved residues were identified that maintain similar tertiary structures.
Li
Ming
M
"Tsinghua-Nankai-IBP Joint Research Group for Structural Biology", Tsinghua University, Beijing 100084, China.
Li
Shentao
S
Lou
Zhiyong
Z
Liao
Xiaoping
X
Zhao
Xiaodong
X
Meng
Zhaohui
Z
Bartlam
Mark
M
Rao
Zihe
Z
D018360:Crystallography, X-Ray; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry*; D009124:Muscle Proteins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D008840:Microfilament Proteins; D009124:Muscle Proteins; C051615:transgelin