title: Anomalous adsorptive properties of HIV protease: indication of two-dimensional crystallization?
abstract: Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.
authors.affiliation: Institute of Chemical Technology, Department of Analytical Chemistry, Technická 5, 166 28 Prague 6, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Cígler
authors.forename: Vladimír
authors.initials: V
authors.lastname: Král
authors.forename: Milan
authors.initials: M
authors.lastname: Kozísek
authors.forename: Jan
authors.initials: J
authors.lastname: Konvalinka
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Mirsky
mesh_terms: D000327:Adsorption; D003460:Crystallization; D006046:Gold; D006678:HIV / Q000737:chemistry / Q000201:enzymology*; D016333:HIV Protease / Q000737:chemistry* / Q000493:pharmacokinetics*; D006801:Humans; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D006046:Gold; D016333:HIV Protease