title: Validation of COBAS Taqman CT for the detection of Chlamydia trachomatis in vulvo-vaginal swabs.
abstract: BACKGROUND
  Vulvo-vaginal swabs (VVSs) are not validated for use by the manufacturers of two widely used nucleic acid amplification tests (NAAT) for the detection of Chlamydia trachomatis. However, there is evidence that this type of swab is suitable for diagnosis.
  
  
  OBJECTIVE
  To validate the Cobas Taqman CT assay for the detection of C trachomatis in VVS.
  
  
  METHODS
  Women aged 18-24 years attending a genitourinary medicine clinic were invited to take part in the study. Participants provided a self-taken VVS and the results obtained with these samples were compared with those obtained with an endocervical swab collected by a healthcare worker. A total of 267 women took part.
  
  
  RESULTS
  255/267 (96%; 95% CI 92 to 98%) sets of samples gave concordant results. 12/267 (4.5%) VVSs were invalid/inhibitory and so no result was available for these samples. This compared with 2/267 (0.7%) for endocervical swabs.
  
  
  CONCLUSIONS
  VVS are suitable samples for detecting C trachomatis.
authors.affiliation: Department of Microbiology, Princess Royal Hospital, Telford TF1 1TF, UK. sue.skidmore@sath.nhs.uk
authors.forename: S
authors.initials: S
authors.lastname: Skidmore
authors.forename: M
authors.initials: M
authors.lastname: Kaye
authors.forename: D
authors.initials: D
authors.lastname: Bayliss
authors.forename: S
authors.initials: S
authors.lastname: Devendra
mesh_terms: D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D012648:Self Care; D013048:Specimen Handling / Q000379:methods / Q000592:standards; D019914:Taq Polymerase; D014626:Vaginal Smears / Q000379:methods; D014848:Vulvovaginitis / Q000382:microbiology
chemical_list: D019914:Taq Polymerase