title: Synthesis and photochemical properties of a light-activated fluorophore to label His-tagged proteins.
abstract: Rapid and efficient light-induced fluorescence enhancement is demonstrated on a DMNPB-"caged" coumarin derivative carrying a His-tag recognition motif.
authors.affiliation: Universtté Louis Pasteur, CNRS UMR 7175-Laboratoire de chimie bioorganique, Faculté de pharmacie, 74 route du rhin, 67400 Illkirch, France.
authors.forename: Clélia
authors.initials: C
authors.lastname: Orange
authors.forename: Alexandre
authors.initials: A
authors.lastname: Specht
authors.forename: David
authors.initials: D
authors.lastname: Puliti
authors.forename: Elias
authors.initials: E
authors.lastname: Sakr
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Furuta
authors.forename: Barbara
authors.initials: B
authors.lastname: Winsor
authors.forename: Maurice
authors.initials: M
authors.lastname: Goeldner
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D008027:Light; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D006639:Histidine