Properties of TiO2-polyacrylic acid dispersions with potential for molecular recognition.
Titanium dioxide (TiO2)/polyacrylic acid (PAA) (TiO2/PAA) particles were formed by mixing PAA and an acidic solution of TiO2 nanoparticles in dimethylformamide (DMF) followed by heat treatment. TEM and particle analysis showed that the resulting particles had a narrow size distribution. The colloid was very stable and aggregation was not observed over a wide pH range (3-9) or at high salt concentration. The residual carboxylic acid of PAA could be modified via EDC/NHS activation to form an amide bond with a protein. An antibody was attached to the hybrid nanoparticle and specific binding to antigen was monitored by surface plasmon resonance. The results suggest that TiO2/PAA nanoparticles are candidates as the base component of a photocatalytic system with potential for substrate selectivity.
Kanehira
Koki
K
TOTO Ltd. Research Institute, Biotechnology Group, Nakashima 2-1-1, Kokurakita, Kitakyushu, Fukuoka 802-8601, Japan.
Banzai
Toshiaki
T
Ogino
Chiaki
C
Shimizu
Nobuaki
N
Kubota
Yoshinobu
Y
Sonezaki
Shuji
S
D000180:Acrylic Resins / Q000737:chemistry* / Q000378:metabolism; D001666:Binding Sites, Antibody; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D020349:Surface Plasmon Resonance; D014025:Titanium / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
D000180:Acrylic Resins; C009495:titanium dioxide; C006903:carbopol 940; D014025:Titanium