title: [Optimization of preparative separation and purification of water-soluble substance from Salvia miltiorrhiza by macroporous resins].
abstract: OBJECTIVE
  To study the optimum conditions for the isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge.
  
  
  METHODS
  The optimum macroporous resin was selected and the separation and purification process was evaluated by measuring the content of Salvianolic acid B in the fractions by HPLC.
  
  
  RESULTS
  The XDA-5 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 18 mg/ml corresponding to Salvianolic acid B for concentration of extract, pH was 4, and the volume of 70% (V/V) ethanol as eluant was 3 BV. By this method, the elution efficiency of Salvianolic acid B exceeded 90%.
  
  
  CONCLUSIONS
  The method is more effective for large-scale isolation and purification of water-soluble substance from Salvia miltiorrhiza Bge.
authors.affiliation: Institute of Materia Medica, Guangdong College of Pharmacy, Guangzhou 510006, China. weih8201@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Shen
authors.forename: Li-min
authors.initials: LM
authors.lastname: Sun
mesh_terms: D001572:Benzofurans / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D014867:Water; D053630:Depsides
chemical_list: D001572:Benzofurans; D000431:Ethanol; D012117:Resins, Synthetic; D014867:Water; C076944:salvianolic acid B; D053630:Depsides