Functional characterization of a wheat plasma membrane Na+/H+ antiporter in yeast.
The functional analysis of the sodium exchanger SOS1 from wheat, TaSOS1, was undertaken using Saccharomyces cerevisiae as a heterologous expression system. The TaSOS1 protein, with significant sequence homology to SOS1 sodium exchangers from Arabidopsis and rice, is abundant in roots and leaves, and is induced by salt treatment. TaSOS1 suppressed the salt sensitivity of a yeast strain lacking the major Na+ efflux systems by decreasing the cellular Na+ content while increasing K+ content. Na+/H+ exchange activity of purified plasma membrane from yeast cells expressing TaSOS1 was higher than controls transformed with empty vector. These results demonstrate that TaSOS1 contributes to plasma membrane Na+/H+ exchange.
Xu
Haixia
H
College of Agronomy, Henan Agricultural University, Wenhua Road 95, Zhengzhou, Henan 450002, China.
Jiang
Xingyu
X
Zhan
Kehui
K
Cheng
Xiyong
X
Chen
Xinjian
X
Pardo
Jose M
JM
Cui
Dangqun
D
D002462:Cell Membrane / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D012965:Sodium Chloride / Q000633:toxicity; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D014908:Triticum / Q000187:drug effects / Q000235:genetics / Q000502:physiology*
D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride