title: Tumour necrosis factor (TNF-alpha) alpha converting enzyme and soluble TNF-alpha receptor type 1 in psoriasis patients in relation to the chronic alcohol consumption.
abstract: BACKGROUND
  Tumour necrosis factor alpha converting enzyme (TACE) is a major sheddase of TNF-alpha and its receptors, essential for the generation of soluble, mature molecules. The regulation of the TACE activity by ethanol in vitro has been suggested recently. The alcohol abuse is a frequent problem among psoriasis patients. The aim of the study was to analyse the relationship between long-term alcohol consumption and the concentration of TACE in peripheral blood mononuclear cells (PBMC) and its substrate--soluble TNF-alpha receptor type 1 (sTNF-R1) in plasma in psoriasis patients.
  
  
  METHODS
  The study has been conducted among 44 patients (aged 30-59 years) with early-onset, plaque-type psoriasis. Thirty-eight patients (aged 29-61 years) with other than psoriasis chronic dermatologic disorders were controls. The data on alcohol consumption during previous 10 years were obtained with a structured questionnaire. The severity of the disease was assessed using Psoriasis Area and Severity Index (PASI), and concentrations of TACE in PBMC lysate and sTNF-R1 in plasma was assessed with a quantitative sandwich enzyme immunoassay technique.
  
  
  RESULTS
  The TACE concentration correlated to that of sTNF-R1 (R = 0.52 in psoriasis patients and R = 0.56 in controls, P < 0.05). The concentrations of TACE were 2.62 +/- 0.32 ng/mL in patients and 1.29 +/- 0.25 ng/mL in controls (P < 0.05), and corresponding sTNF-R1 concentrations were 2.54 +/- 0.27 ng/mL and 1.79 +/- 0.14 ng/mL (P < 0.05), respectively. The concentrations of TACE and sTNF-R1 in patients correlated to the intensity of alcohol consumption (R = 0.56, and R = 0.52, P < 0.05, respectively) and were the highest in excessive drinking psoriasis patients (2.94 +/- 0.34 and 2.67 +/- 0.13 ng/mL).
  
  
  CONCLUSIONS
  The alcohol abuse may contribute to the increase of TACE expression in PBMC and also to the elevated plasma sTNF-R1 concentration in psoriasis patients.
authors.affiliation: Department of Dermatology and Venereology, Medical University of Bialystok, Bialystok, Poland.
authors.forename: A B
authors.initials: AB
authors.lastname: Serwin
authors.forename: M
authors.initials: M
authors.lastname: Sokolowska
authors.forename: E
authors.initials: E
authors.lastname: Dylejko
authors.forename: B
authors.initials: B
authors.lastname: Chodynicka
mesh_terms: D051722:ADAM Proteins / Q000097:blood*; D000072198:ADAM17 Protein; D000328:Adult; D000428:Alcohol Drinking; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000378:metabolism*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood*
chemical_list: D047888:Receptors, Tumor Necrosis Factor, Type I; D051722:ADAM Proteins; D000072198:ADAM17 Protein; C000606462:ADAM17 protein, human