title: [Effect of co-culture of bone marrow mesenchymal stem cells and K562 cells on expression of interleukin 8].
abstract: To investigate the effects of interaction between human bone marrow mesenchymal stem cells (MSCs) and K562 cells on the expression of proangiogenic factor IL-8, the K562 cells were co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium while the controlled K562 cells were cultured alone. The IL-8 gene expression of K562 cells and MSCs was determined by RT-PCR. The results indicated that the expression of IL-8 in K562 cells when co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium was obviously higher than that of K562 cells cultured alone (p<0.01). MSCs co-cultured with K562 cells also had an increased level of IL-8 compared with MSCs cultured alone (p<0.01). It is concluded that the interaction between MSCs and K562 cells via direct contact and the cytokine network promotes expression of IL-8.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Fei
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Wu
authors.forename: Cheng-Ya
authors.initials: CY
authors.lastname: Wang
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D018920:Coculture Techniques; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D012333:RNA, Messenger