Functional analyses of neutrophil-like differentiated cell lines under a hyperglycemic condition.
Diabetic patients are prone to severe bacterial infections. The functional alterations of neutrophils by hyperglycemia are thought to be partially responsible for such infections. In this study, we investigated the functional changes of neutrophil-like differentiated cell lines (dHL-60, dTHP-1, and dNB-4) by treatment with 5.5 mM, 11 mM, or 35 mM of glucose. In dHL-60 cells, the incubation with high glucose (35 mM) resulted in the enhancement of cell aggregation, the suppression of cellular fragility, the induction of reactive-oxygen species (ROS) production by phorbol myristate acetate (PMA) stimulation, and the impairment of phagocytosis. In dTHP-1 cells, the treatment with higher glucose generated the suppression of cellular fragility and extremely impaired phagocytosis (by 35 mM), and induced ROS production due to PMA stimulation (by 11 mM). Furthermore, the higher glucose exposure to dNB-4 cells enlarged intracellular vacuoles (by 35 mM) and induced ROS production due to PMA stimulation (by 11 mM). Since the ROS generation of those cells was enhanced only after PMA stimulation under the higher glucose conditions, glucose may have a priming effect rather than a triggering effect. These extraordinary sensitivities caused by the higher glucose treatments may reflect the dysfunction or overactivation of neutrophils.
Oya-Ito
Tomoko
T
Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, Japan.
Naitou
Hirotaka
H
Masuda
Shuichi
S
Kinae
Naohide
N
Ohashi
Norio
N
D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005947:Glucose / Q000008:administration & dosage; D018922:HL-60 Cells; D006801:Humans; D006943:Hyperglycemia / Q000503:physiopathology*; D007951:Leukemia, Myeloid; D008353:Mannitol / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014617:Vacuoles / Q000187:drug effects
D017382:Reactive Oxygen Species; D008353:Mannitol; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate