Expression of steroidogenic enzymes and synthesis of steroid hormones during development of ovarian follicles in prepubertal goats.
Expression of mRNAs encoding cytochrome P450 side-chain cleavage (P450scc), cytochrome P450 17 alpha-hydroxylase (P450c17), and cytochrome P450 aromatase (P450arom) were characterized by the RT-PCR technique and concentrations of progesterone (P4), testosterone (T0) and estradiol (E2) were measured by radioimmunoassay during follicular development of prepubertal goats. Synthesis of mRNAs encoding P450scc and P450c17 began in preantral follicles, but mRNA encoding P450arom was not detectable until early antral formation. While mRNA for P450scc was expressed in both theca and granulosa cells, mRNA for P450c17 was expressed only in theca cells while P450arom mRNA only in granulosa cells. In nonatretic follicles from prepubertal ovaries, the relative quantity of mRNA expression of all the three enzymes increased with follicle size; however, while the concentration of P4 and E2 increased, that of T0 decreased with follicle size. While expression of mRNA encoding P450scc was unaffected, that of P450c17 mRNA decreased to the lowest level and mRNA for P450arom became undetectable following atresia; accordingly, while the concentration of P4 increased in the atretic medium follicles, that of T0 and E2 decreased to the lowest level after atresia. While the adult follicular stage follicles showed a similar cytochrome expression as the nonatretic follicles of prepubertal goats, the former contained higher levels of E2 and P4 than the latter. The presence of corpus luteum in an ovary decreased expression of P450scc, significantly in large follicles while it increased concentration of P4. These findings indicated that (1) similar to other species, changes in follicular steroid production in goats were explained in large measure by changes in steroidogenic enzyme expression; (2) while mRNA expression was similar, activities of some of the steroidogenic enzymes may differ between sexually mature and immature goats.
Yuan
Ji-Hong
JH
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City 271018, PR China.
Wang
Jun-Zuo
JZ
Lan
Guo-Cheng
GC
Sui
Hong-Shu
HS
Yu
Jian-Ning
JN
Tan
Jing-He
JH
D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006041:Goats / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis* / Q000097:blood; D006080:Ovarian Follicle / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012741:Sexual Maturation / Q000235:genetics*; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism
D012739:Gonadal Steroid Hormones; D012333:RNA, Messenger; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme