title: Expression of bacterial genes and induction of INF-gamma in human myeloid dendritic cells during persistent infection with Chlamydophila pneumoniae.
abstract: The interactions between human monocyte-derived dendritic cells (DCs) and Chlamydophila pneumoniae (Cpn) infection were investigated. Cpn infection induced the maturation and functional activation of DCs, and Cpn antigens were present in all of the subpopulations during the maturation process. Chlamydial antigens were detected in DCs during an observation period of 28 days. The exponential production of infectious elementary bodies was not observed. Chlamydial transcripts of the 16S rRNA gene, groEL-1 and omcB genes were expressed, as determined by a quantitative real-time PCR, but the expression of the ftsK gene was limited. DC cultures produced IFN-gamma, but the presence of IFN-gamma in the culture medium was not the major factor that decreased the growth of Cpn, as was shown by neutralization of the IFN-gamma. A cell population identified as producing IFN-gamma had no markers for T, B, natural killer, monocyte cells or macrophages but displayed DC morphology and the expression of specific DC markers, such as CD11c and HLA-DR. These results reveal a persistent infection of DCs with the expression of some, but not cell division-related genes and the production of IFN-gamma that may contribute to the pathomechanism of chronic inflammatory diseases associated with persistent Cpn infection.
authors.affiliation: National Center for Epidemiology, Budapest, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kis
authors.forename: Balint
authors.initials: B
authors.lastname: Treso
authors.forename: Katalin
authors.initials: K
authors.lastname: Burian
authors.forename: Valeria
authors.initials: V
authors.lastname: Endresz
authors.forename: Eva
authors.initials: E
authors.lastname: Pallinger
authors.forename: Agnes
authors.initials: A
authors.lastname: Nagy
authors.forename: Akos
authors.initials: A
authors.lastname: Toth
authors.forename: Maria
authors.initials: M
authors.lastname: Takacs
authors.forename: Andras
authors.initials: A
authors.lastname: Falus
authors.forename: Eva
authors.initials: E
authors.lastname: Gonczol
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000235:genetics; D002478:Cells, Cultured; D023521:Chlamydophila Infections / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D016207:Cytokines / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D005798:Genes, Bacterial; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D022423:Myeloid Cells; D011994:Recombinant Proteins; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D016207:Cytokines; D011994:Recombinant Proteins; D007371:Interferon-gamma