Suppressive effects of Anoectochilus formosanus extract on osteoclast formation in vitro and bone resorption in vivo.
Anoectochilus formosanus, a plant native to Taiwan, is used as a folk medicine. It was found that oral administration of A. formosanus extract (AFE) (500 mg/kg) for 4 weeks suppressed bone weight loss and trabecular bone loss in ovariectomized mice, an experimental model of osteoporosis. Although AFE at 12.5 and 25 mug/ml inhibited osteoclast formation in co-culture of osteoblasts and bone marrow cells, AFE did not inhibit the formation of osteoclast progenitor cells and preosteoclast cells in bone marrow cells and RAW264 cells. However, AFE (at 12.5 and 25 microg/ml) decreased RANKL expression. These results suggested that AFE might suppress the bone loss caused by estrogen deficiency through suppression of RANKL expression required for osteoclast formation.
Masuda
Kikuko
K
Laboratory of Nutraceuticals and Functional Foods Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minatoku, Tokyo 108-8477, Japan.
Ikeuchi
Mayumi
M
Koyama
Tomoyuki
T
Yamaguchi
Kohji
K
Woo
Je-Tae
JT
Nishimura
Tomio
T
Yazawa
Kazunaga
K
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001862:Bone Resorption / Q000188:drug therapy* / Q000473:pathology*; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D005269:Femur / Q000187:drug effects / Q000473:pathology; D051379:Mice; D029595:Orchidaceae; D009929:Organ Size / Q000187:drug effects; D010010:Osteoclasts / Q000166:cytology* / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D010052:Ovariectomy; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D053245:RANK Ligand / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects
D010936:Plant Extracts; D053245:RANK Ligand; D002117:Calcitriol