Short communication: characterization of a kappa-casein genetic variant in the Chinese yak, Bos grunniens.
A PCR-single strand conformation polymorphism protocol has been developed for rapid genotyping of the yak kappa-casein gene. A total of 307 yaks from the Tianzhu White, Jiulong, Maiwa, and Datong breeds in China were genotyped at the kappa-casein locus using the protocol developed in the present study. A polymorphism of kappa-casein gene exon 4 has been identified in Tianzhu White breed by evaluating genomic DNA. The polymorphic site consists of a single nucleotide substitution G-->C at position 362 of the exon 4, resulting in an AA substitution from Arg to Pro at position 121 of the AA sequence and in 2 alleles named, respectively, G and C based on nucleotide 362. The occurrence of allele C in the Tianzhu White breed was high with an allele frequency of 0.15. However, allele C appears to be absent in the yaks from Jiulong, Maiwa, and Datong breeds.
Bai
W L
WL
Animal Science and Veterinary Medicine College, Shenyang Agricultural University, Shenyang 110161, China. wlb7411@163.com <wlb7411@163.com>
Yin
R H
RH
Zhao
S J
SJ
Zheng
Y C
YC
Zhong
J C
JC
Zhao
Z H
ZH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002681:China; D004247:DNA / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D013045:Species Specificity
D002364:Caseins; D004247:DNA