title: cis-Preferential requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1.
abstract: The genome of Red clover necrotic mosaic virus (RCNMV) consists of RNA1 and RNA2. RNA1 encodes N-terminally overlapping replication proteins, p27 and p88, which are translated in a cap-independent manner. The 3' untranslated region of RNA1 contains RNA elements essential for cap-independent translation and negative-strand RNA synthesis. In this study, we investigated how p27 and p88 were engaged in the replication of RCNMV genomic RNAs by using DNA vectors or in vitro transcribed RNAs in protoplasts and in a cell-free extract of evacuolated BY-2 protoplasts. Our results show a cis-preferential requirement of p88, but not of p27, for the replication of RNA1. This mechanism might help to facilitate a switch in the role of RNA1 from mRNA to a replication template.
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
authors.forename: Kimiyuki
authors.initials: K
authors.lastname: Okamoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagano
authors.forename: Hirooki
authors.initials: H
authors.lastname: Iwakawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizumoto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Takeda
authors.forename: Masanori
authors.initials: M
authors.lastname: Kaido
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Mise
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Okuno
mesh_terms: D015152:Blotting, Northern; D007887:Fabaceae; D018965:Frameshifting, Ribosomal; D011485:Protein Binding; D011523:Protoplasts; D012367:RNA, Viral / Q000378:metabolism; D019183:Tombusviridae / Q000502:physiology*; D014158:Transcription, Genetic; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication / Q000502:physiology*
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins