Production and characterization of monoclonal antibody to botulinum neurotoxin type B light chain by phage display.
A monoclonal antibody to the light chain of botulinum neurotoxin type B (BoNT/B) was generated and its protective activity was evaluated in vivo. A chimeric rabbit/human Fab library was generated using bone marrow and spleen cDNAs of rabbits immunized with the BoNT/B light chain, and three monoclonal antibodies specific to the catalytic domain of BoNT/B were isolated. One of these clones, BCXRH1, was specific to a conformation-dependent epitope, and partially neutralized the BoNT/B complex in vivo.
Lee
Myung-Shin
MS
Department of Microbiology and Immunology, Eulji University School of Medicine, Daejeon, South Korea.
Lee
Ji Chul
JC
Choi
Cha Yong
CY
Chung
Junho
J
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D001853:Bone Marrow / Q000276:immunology; D001905:Botulinum Toxins / Q000276:immunology* / Q000633:toxicity; D019274:Botulinum Toxins, Type A; D020134:Catalytic Domain / Q000276:immunology; D000939:Epitopes; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology* / Q000302:isolation & purification; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019151:Peptide Library; D011817:Rabbits; D013154:Spleen / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A