Effect of brain DHA levels on cytoskeleton expression.
Brain docosahexaenoic acid (DHA, 22:6n-3) levels are associated with learning memory performance, but it is not known the mechanism of DHA on enhancing memory effect. The aim of this study was to examine effect of brain DHA levels on cytoskeleton expression. Rats were fed a chow or sunflower oil-based n-3 fatty acid-deficient diet supplemented with or without fish oil starting from embryo and through postnatal day 140. The various DHA levels were from 5.0% to 15.6% of total fatty acids in hippocampus, 3.9% to 13.7% in visual cortex, and 5.3% to 14.4% in olfactory bulbs. The expression of the cytoskeleton markers tyrosine tubulin, acetylated tubulin, and beta-actin in the hippocampus, visual cortex and olfactory bulb was not affected by brain DHA levels.
Hasio
Ann-Che
AC
Department of Physiology, National Taiwan University College of Medicine, No. 1, Jen-Ai Rd, 1 sec, Taipei, 100, Taiwan.
Chen
Jen-Jui
JJ
Chung
Wan-Ling
WL
Su
Hui-Min
HM
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005395:Fish Oils; D015870:Gene Expression; D007858:Learning / Q000187:drug effects* / Q000502:physiology; D008568:Memory / Q000187:drug effects* / Q000502:physiology; D010938:Plant Oils; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000074242:Sunflower Oil; D014404:Tubulin / Q000378:metabolism
D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D010938:Plant Oils; D000074242:Sunflower Oil; D014404:Tubulin; D004281:Docosahexaenoic Acids