title: Quantification of microRNA by gold nanoparticle probes.
abstract: MicroRNAs are important posttranscriptional regulators of gene expression in animals and plants. A sensitive and specific detection method is urgently needed for intensive studies on differential expression and regulatory roles of microRNA. Here we present a simple and reliable method for the quantification of microRNA. The hybridization products of target microRNA with capture probe and gold nanoparticle probe are immobilized onto the surface of a streptavidin-coated microplate, and the signal is amplified by silver enhancement. Distribution of miR-122a/miR-128 in mouse brain and liver tissue was detected by this method, and synthetic miRNA122a was quantified. This method allowed a lower detect limit of 10 fM with a linear dynamic range from 10 pM to 10 fM and a high specificity to discriminate one single oligonucleotide mismatch of the target microRNA.
authors.affiliation: MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, wuhan 430030, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Yang
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Li
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Li
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Zhao
authors.forename: Wen-Lei
authors.initials: WL
authors.lastname: He
authors.forename: Yu-Qiu
authors.initials: YQ
authors.lastname: Gao
authors.forename: Yan-Jian
authors.initials: YJ
authors.lastname: Wan
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Tian
authors.initials: T
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.forename: Shun-Qing
authors.initials: SQ
authors.lastname: Xu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D036103:Nanotechnology / Q000379:methods; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D006046:Gold