Peroxynitrite inhibits electron transport on the acceptor side of higher plant photosystem II.
Peroxynitrite is a strong oxidant that has been proposed to form in chloroplasts. The interaction between peroxynitrite and photosystem II (PSII) has been investigated to determine whether this oxidant could be a hazard for PSII. Peroxynitrite is shown to inhibit oxygen evolution in PSII membranes in a dose-dependent manner. Analyses by PAM fluorimetry and EPR spectroscopy have demonstrated that the inhibition target of peroxynitrite is on the PSII acceptor side. In the presence of the herbicide DCMU, the chlorophyll (Chl) a fluorescence induction curve is inhibited by peroxynitrite, but the slow phase of the Chl a fluorescence decay does not change. EPR studies demonstrate that the Signal II(slow) and Signal II(fast) of peroxynitrite-treated Tris-washed PSII membranes are induced at room temperature, implying that the redox active tyrosines Y(Z) and Y(D) of PSII are not significantly nitrated. A featureless EPR signal with a g value of approximately 2.0043+/-0.0003 and a line width of 10+/-1G is induced under continuous illumination in the presence of peroxynitrite. This new EPR signal corresponds with the semireduced plastoquinone Q(A) in the absence of magnetic interaction with the non-heme Fe2+. We conclude that peroxynitrite impairs PSII electron transport in the Q(A)Fe2+ niche.
González-Pérez
Sergio
S
Departamento de Estrés Abiótico, Instituto de Recursos Naturales y Agrobiología de Salamanca (IRNASA-CSIC), Apdo. 257, 37071 Salamanca, Spain.
Quijano
Celia
C
Romero
Natalia
N
Melø
Thor Bernt
TB
Radi
Rafael
R
Arellano
Juan B
JB
D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D004237:Diuron / Q000737:chemistry / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000502:physiology; D005296:Ferrous Compounds / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D016877:Oxidants / Q000737:chemistry / Q000502:physiology; D010100:Oxygen / Q000378:metabolism; D030421:Peroxynitrous Acid / Q000737:chemistry / Q000502:physiology*; D045332:Photosystem II Protein Complex / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D011048:Polarography; D011809:Quinones / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D018724:Spinacia oleracea / Q000737:chemistry / Q000378:metabolism
D005296:Ferrous Compounds; D016877:Oxidants; D045332:Photosystem II Protein Complex; D011809:Quinones; D002734:Chlorophyll; D030421:Peroxynitrous Acid; D004237:Diuron; D010100:Oxygen; D000077194:Chlorophyll A