MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines.
Micropthalmia-associated transcription factor (MITF) is the master regulator of melanocyte development, survival, and function. Frequent alteration in the expression of MITF is detected in melanoma, but the mechanism(s) underlying the alteration in expression have not been completely determined. In these studies, we have identified microRNA-137 (miR-137) as a regulator of MITF expression. The genomic locus of miR-137 at chromosome 1p22 places it in a region of the human genome previously determined to harbor an allele for melanoma susceptibility. Here, we show that expression of mature miR-137 in melanoma cell lines down-regulates MITF expression. Further, we have identified a 15-bp variable nucleotide tandem repeat located just 5' to the pre-miR-137 sequence, which alters the processing and function of miR-137 in melanoma cell lines.
Bemis
Lynne T
LT
Division of Medical Oncology, University of Colorado Denver School of Medicine, Aurora, CO 80045, USA.
Chen
Robert
R
Amato
Carol M
CM
Classen
Elizabeth H
EH
Robinson
Steven E
SE
Coffey
David G
DG
Erickson
Paul F
PF
Shellman
Yiqun G
YG
Robinson
William A
WA
D020413:3' Untranslated Regions; D000483:Alleles; D001483:Base Sequence; D045744:Cell Line, Tumor; D002874:Chromosome Mapping; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D014158:Transcription, Genetic
D020413:3' Untranslated Regions; D035683:MicroRNAs; D051739:Microphthalmia-Associated Transcription Factor