An antimicrobial peptide modulates epithelial responses to bacterial products.
BACKGROUND
Changes in the respiratory epithelium and chronic and recurrent infections are thought to play a central role in the pathogenesis of otitis media and sinusitis. The airway epithelium is the primary defense system of the respiratory tract. Bacterial cell membrane components like lipopolysaccharide (LPS) and lipoteichoic acid (LTA) can affect the mucociliary clearance function of the respiratory epithelium. P60.4-Ac is a synthetic antimicrobial peptide based on the structure of the cathelicidin LL-37 that neutralizes the pro-inflammatory activity of LPS and LTA.


METHODS
Normal respiratory sinus epithelium was cultured at the air liquid interface. The cells were incubated with LPS or LTA in the presence or absence of P60.4-Ac.


RESULTS
P60.4-Ac neutralized the LPS- and LTA- induced effect on air-liquid interface cultured epithelial cells. P60.4-Ac significantly inhibited the increase in the epithelial layer caused by LPS or LTA.


CONCLUSIONS
These data demonstrate that P60.4-Ac might be of clinical benefit in the management of otitis media with effusion and sinusitis.
Vonk
Marcel J
MJ
Department of Otorhinolaryngology, Leiden University Medical Center, Leiden, The Netherlands. m.j.vonk@lumc.nl
Hiemstra
Pieter S
PS
Grote
Jan J
JJ
D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology* / Q000627:therapeutic use*; D009079:Mucociliary Clearance / Q000187:drug effects; D010033:Otitis Media / Q000188:drug therapy / Q000382:microbiology; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000382:microbiology*; D012852:Sinusitis / Q000188:drug therapy / Q000382:microbiology; D013101:Sphenoid Sinus / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D013682:Teichoic Acids / Q000494:pharmacology* / Q000627:therapeutic use*; D054804:Cathelicidins
D023181:Antimicrobial Cationic Peptides; D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D054804:Cathelicidins