[Experimental study on human bone marrow mesenchymal stem cells transfected by SDF-1 cDNA].
This study was aimed to investigate the expression of SDF-1 mRNA in SDF-1 cDNA-modified human bone marrow mesenchymal stem cells (hBMSCs) before and after transfection. The hBMSCs were isolated, cultured and identified, the SDF-1-pIRES2-EGFP eukaryotic expressing vector was constructed, and then the hBMSCs were transfected with the vector encapsulated by lipofectamine 2000. The transfection efficiency was measured by observing the expression of green fluorescence protein and detecting the mRNA by RT-PCR. The results indicated that the expression of SDF-1 mRNA increased by about 20% after hBMSCs were transfected instantaneously by SDF-1-pIRES2-EGFP. It is concluded that SDF-1 cDNA eukaryotic expression vector can be instantly transfected into hBMSCs by lipofectamine 2000, but the efficiency was too low to obtain enough steady transferred hBMSCs. Other procedures should be trialed to improve the transfection efficiency.
Liang
Xue
X
Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China.
Su
Yong-Ping
YP
Kong
Pei-Yan
PY
Chen
Xing-Hua
XH
Peng
Xian-Gui
XG
Xu
Hui
H
Ai
Guo-Ping
GP
D001854:Bone Marrow Cells / Q000166:cytology*; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection
C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D018076:DNA, Complementary; D012333:RNA, Messenger