title: Functional analyses of neutrophil-like differentiated cell lines under a hyperglycemic condition.
abstract: Diabetic patients are prone to severe bacterial infections. The functional alterations of neutrophils by hyperglycemia are thought to be partially responsible for such infections. In this study, we investigated the functional changes of neutrophil-like differentiated cell lines (dHL-60, dTHP-1, and dNB-4) by treatment with 5.5 mM, 11 mM, or 35 mM of glucose. In dHL-60 cells, the incubation with high glucose (35 mM) resulted in the enhancement of cell aggregation, the suppression of cellular fragility, the induction of reactive-oxygen species (ROS) production by phorbol myristate acetate (PMA) stimulation, and the impairment of phagocytosis. In dTHP-1 cells, the treatment with higher glucose generated the suppression of cellular fragility and extremely impaired phagocytosis (by 35 mM), and induced ROS production due to PMA stimulation (by 11 mM). Furthermore, the higher glucose exposure to dNB-4 cells enlarged intracellular vacuoles (by 35 mM) and induced ROS production due to PMA stimulation (by 11 mM). Since the ROS generation of those cells was enhanced only after PMA stimulation under the higher glucose conditions, glucose may have a priming effect rather than a triggering effect. These extraordinary sensitivities caused by the higher glucose treatments may reflect the dysfunction or overactivation of neutrophils.
authors.affiliation: Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Oya-Ito
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Naitou
authors.forename: Shuichi
authors.initials: S
authors.lastname: Masuda
authors.forename: Naohide
authors.initials: N
authors.lastname: Kinae
authors.forename: Norio
authors.initials: N
authors.lastname: Ohashi
mesh_terms: D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005947:Glucose / Q000008:administration & dosage; D018922:HL-60 Cells; D006801:Humans; D006943:Hyperglycemia / Q000503:physiopathology*; D007951:Leukemia, Myeloid; D008353:Mannitol / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014617:Vacuoles / Q000187:drug effects
chemical_list: D017382:Reactive Oxygen Species; D008353:Mannitol; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate