T cell epitope-based peptide-DNA dual vaccine induces protective immunity against Schistosoma japonicum infection in C57BL/6J mice.
Schistosomiasis is a major public health problem that primarily affects developing countries. Although schistosomicidal drugs exist, the development of an efficacious vaccine would potentially be the most powerful means of controlling this disease. Previous studies have shown that vaccination with selected protective epitopes successfully induced partial protection and/or reduced female fecundity in animal models. Thus, we investigated whether the T cell epitope P5 from the host-interactive tegument of Schistosoma japonicum 22.6 (S. japonicum) could act as a protective epitope. The protective potential of P5 in a vaccine against S. japonicum was determined by using a T cell epitope based peptide-DNA dual vaccine (PDDV). In our experiments, the vaccine construct (P5-18K-PDDV) contains the peptide of the T cell epitope (P5) and plasmid DNA, encoding P5 and adjuvant GM-CSF. We show that P5-18K-PDDV induced both cell-mediated and humoral immune responses in vivo and achieved partial protection against S. japonicum infection in C57BL/6J mice. Histopathological studies reveal that P5-18K-PDDV immunized mice had substantially reduced liver pathology compared to the control groups. Together, these results suggest that P5 could be used as a vaccine immunogen for both worm killing and disease prevention against S. japonicum.
Zhang
Lei
L
Department of Pathogen Biology & Immunology, Jiangsu Province Laboratory of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, PR China.
Yang
Yanfen
Y
Yang
Xue
X
Zhao
Jiaqing
J
Yang
Jianghua
J
Liu
Feng
F
Zhang
Zhaosong
Z
Wu
Guanling
G
Su
Chuan
C
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D007279:Injections, Subcutaneous; D008099:Liver / Q000473:pathology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D013154:Spleen / Q000276:immunology; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage
D000909:Antibodies, Helminth; D018984:Epitopes, T-Lymphocyte; D014612:Vaccines; D019444:Vaccines, DNA