Development of novel magnetic nano-carriers for high-performance affinity purification.
We developed novel magnetic nano-carriers around 180 nm in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nano-carriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein.
Nishio
Kosuke
K
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Masaike
Yuka
Y
Ikeda
Morihito
M
Narimatsu
Hiroki
H
Gokon
Nobuyuki
N
Tsubouchi
Shingo
S
Hatakeyama
Mamoru
M
Sakamoto
Satoshi
S
Hanyu
Naohiro
N
Sandhu
Adarsh
A
Kawaguchi
Haruma
H
Abe
Masanori
M
Handa
Hiroshi
H
D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage; D002846:Chromatography, Affinity; D016503:Drug Delivery Systems; D004852:Epoxy Compounds / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D008027:Light; D008280:Magnetics; D008689:Methacrylates / Q000737:chemistry; D008727:Methotrexate / Q000008:administration & dosage; D008863:Microspheres; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D020058:Styrene / Q000737:chemistry; D013818:Thermogravimetry
D000964:Antimetabolites, Antineoplastic; D004852:Epoxy Compounds; D005290:Ferric Compounds; D008689:Methacrylates; D011108:Polymers; C001215:ferrite; D020058:Styrene; C007870:glycidyl methacrylate; D008727:Methotrexate