In situ biomonitoring of PAH-contaminated sediments using juvenile coho salmon (Oncorhynchus kisutch).
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous marine and freshwater sediment contaminants. Extensive data exist to confirm that PAHs are toxic to aquatic receptors. However, limited information is available regarding the bioavailability and genotoxicity of sediment PAHs to aquatic organisms. This study investigated an integrated biomonitoring approach using chemical analyses and biomarkers to characterize the bioavailability and genotoxicity of a complex PAH mixture in freshwater lake sediments associated with a former manufactured gas plant (MGP). Sediment PAH genotoxicity was assessed by flow cytometry (FCM), DNA adduct (32)P-postlabeling, and erythrocyte micronuclei in juvenile coho salmon (Oncorhynchus kisutch) caged in the water column. Significant PAH-induced genotoxicity was observed with FCM and (32)P-postlabeling, but not with erythrocyte micronuclei. Chromosome damage in peripheral blood and hepatic DNA adducts correlated with sediment, but not water column PAH concentrations. Total hepatic DNA adducts in salmon caged nearest the former MGP facility was 39+/-6.5 (RALx10(9)), while salmon caged in a reference lake had 28+/-2.3 total hepatic DNA adducts per 10(9) nucleotides. These results indicate that in situ biomonitoring using biomarkers and caged fish can be a sensitive indicator of genotoxic PAHs in sediments.
Barbee
Gary C
GC
Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843-4458, USA.
Barich
John
J
Duncan
Bruce
B
Bickham
John W
JW
Matson
Cole W
CW
Hintze
Christopher J
CJ
Autenrieth
Robin L
RL
Zhou
Guo-Dong
GD
McDonald
Thomas J
TJ
Cizmas
Leslie
L
Norton
Dale
D
Donnelly
Kirby C
KC
D000818:Animals; D015415:Biomarkers; D018736:DNA Adducts; D004784:Environmental Monitoring / Q000379:methods*; D005618:Fresh Water / Q000737:chemistry; D019015:Geologic Sediments / Q000737:chemistry*; D008099:Liver / Q000187:drug effects; D009152:Mutagenicity Tests; D017684:Oncorhynchus kisutch / Q000254:growth & development*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D015415:Biomarkers; D018736:DNA Adducts; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical