Unique expression of a small IL-32 protein in the Jurkat leukemic T cell line.
Interleukin (IL)-32 was recently identified as a new cytokine which induces various proinflammatory cytokines in human monocytes and macrophages. Therefore, IL-32 has been primarily studied in inflammatory models such as rheumatoid arthritis and inflammatory bowel diseases. The regulation of endogenous IL-32 in other immune cells remains unknown. In the present study, we stimulated Jurkat T cells with phytohaemagglutinin (PHA) and phorbol myristate acetate (PMA) and examined IL-32 expression at both the mRNA and protein levels. All mRNAs of the four IL-32 isoforms and the 12-15 kDa IL-32 protein were independent of PHA and PMA stimulation, however a 9 kDa molecular weight IL-32 protein in the cell culture supernatant was induced by PHA and PMA after 16 h of stimulation. Compared to other human cell lines, the Jurkat cell line constitutively expressed a 12-15 kDa molecule of IL-32, which is smaller than the known IL-32 isoforms. We used IL-32 shRNA to examine the specificity of the 12-15 kDa molecule. Upon IL-32 shRNA transfection, the 12-15 kDa band was decreased specifically as compared to the control scrambled clone. Thus, the constitutive expression of IL-32 mRNA as well as the predominant production of a smaller sized IL-32 isoform in Jurkat cells may implicate a role for IL-32 in human T cell leukemia.
Ko
Na-Young
NY
Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, Republic of Korea.
Chang
Sung-Ho
SH
Lee
Jun-Ho
JH
Kim
Nam-Wook
NW
Kim
Young-Mi
YM
Choi
Wahn-Soo
WS
Choi
Ji-Da
JD
Bae
Su-Young
SY
Hong
Jae-Woo
JW
Jaekal
Jun
J
Azam
Tania
T
Her
Erk
E
Kim
Soo-Hyun
SH
D000818:Animals; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D019169:Jurkat Cells; D053146:Myeloblastin / Q000378:metabolism; D010835:Phytohemagglutinins / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000276:immunology
C496971:IL32 protein, human; D007378:Interleukins; D010835:Phytohemagglutinins; D020033:Protein Isoforms; D012333:RNA, Messenger; D053146:Myeloblastin; D013755:Tetradecanoylphorbol Acetate