Polycystin-1 induced apoptosis and cell cycle arrest in G0/G1 phase in cancer cells.
Studies have shown that polycystin-1, encoded by PKD1, the major ADPKD, may have a central role in regulating both apoptosis and proliferation, which could prevent the malignant transformation of affected cells. However, as a putative tumor suppressor, direct studies on the possibility that polycystin-1 may play a role in cancer cells' biological properties have not yet been reported. We have demonstrated that the apoptosis of cancer cells was induced by overexpression of polycystin-1. After transfection with polycystin-1, three cancer cell lines, HepG2, A549, and SW480, showed significantly increased apoptosis compared with the respective control groups. This was accompanied by cell cycle arrest at G(0)/G(1) phase, whereas cell proliferation was not significantly affected. Overexpression of polycystin-1 induces apoptosis in cancer cells, at least partially, through Wnt and a caspase-dependent pathway.
Zheng
Rong
R
Core Facility of Gene Engineered Mouse, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, PR China.
Zhang
Zheng
Z
Lv
Xiaoyan
X
Fan
Junming
J
Chen
Ye
Y
Wang
Yidong
Y
Tan
Ruizhi
R
Liu
Yuhang
Y
Zhou
Qin
Q
D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D016193:G1 Phase; D006801:Humans; D009369:Neoplasms / Q000201:enzymology / Q000473:pathology*; D016192:Resting Phase, Cell Cycle; D015398:Signal Transduction; D050396:TRPP Cation Channels / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics; D051153:Wnt Proteins / Q000378:metabolism
D053148:Caspase 3; D053179:Caspase 7; D050396:TRPP Cation Channels; D051153:Wnt Proteins