Opto-fluidic micro-ring resonator for sensitive label-free viral detection.
We have demonstrated sensitive label-free virus detection using the opto-fluidic ring resonator (OFRR) sensor. The OFRR is a novel sensing platform that integrates the microfluidics and photonic sensing technology with a low detection limit and small volume. In our experiment, filamentous bacteriophage M13 was used as a safe model system. Virus samples were flowed through the OFRR whose surface was coated with M13-specific antibodies. We studied the sensor performance by monitoring in real-time the virus and antibody interaction. It is shown that OFRR can detect M13 with high specificity and sensitivity. The detection limit is approximately 2.3 x 10(3) pfu mL(-1) and the detection dynamic range spanned seven orders of magnitude. Theoretical analysis was also carried out to confirm the experimental results. Our study will lead to development of novel OFRR-based, sensitive, rapid, and low-cost micro total analysis devices for virus detection.
Zhu
Hongying
H
Department of Biological Engineering, University of Missouri-Columbia, 240D, Bond Life Sciences Center, Columbia, MO 65211, USA.
White
Ian M
IM
Suter
Jonathan D
JD
Zourob
Mohammed
M
Fan
Xudong
X
D000914:Antibodies, Viral; D017104:Bacteriophage M13 / Q000302:isolation & purification; D015374:Biosensing Techniques; D004867:Equipment Design; D016014:Linear Models; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D014780:Viruses / Q000302:isolation & purification*
D000914:Antibodies, Viral