title: Single-injection calibration approach for high-performance liquid chromatography.
abstract: A new calibration method for high-performance liquid chromatography was validated. The method was called single-injection calibration approach (SICA) because it allowed to obtain a complete calibration curve by means of a single injection of a standard solution containing several non-volatile and semi-volatile organic compounds at different concentration levels. The compounds studied included carboxylic acids, polyalcohols, carbohydrates and water-soluble vitamins. This method allowed a 1-7-fold reduction in the analysis time with regard to conventional calibration methods. The method was applied to three different chromatographic detection methods: refractive index (RI) detection, diode array detection (DAD) and inductively coupled plasma atomic emission detection (ICP-AED). Good linearity was achieved (r(2)>0.999) for the three detection methods but signal correction was required for RI detection and DAD. This fact demonstrated that ICP-AES was the most universal because the signal obtained for non-volatile and semi-volatile organic compounds was not a function of the chemical nature of the compound and only depended on the mass content of carbon. The method was validated by analyzing a reference non-fat milk powder sample as well as several real food samples (three fruit juices, four wines, three candies and a multivitamin complex).
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Sciences, University of Alicante, 03080 Alicante, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Paredes
authors.forename: Salvador E
authors.initials: SE
authors.lastname: Maestre
authors.forename: Ma Soledad
authors.initials: MS
authors.lastname: Prats
authors.forename: José L
authors.initials: JL
authors.lastname: Todolí
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D009930:Organic Chemicals / Q000032:analysis*
chemical_list: D009930:Organic Chemicals