title: Seasonal variation in transcript abundance in cork tissue analyzed by real time RT-PCR.
abstract: The molecular processes underlying cork biosynthesis and differentiation are mostly unknown. Recently, a list of candidate genes for cork biosynthesis and regulation was made available opening new possibilities for molecular studies in cork oak (Quercus suber L.). Based on this list, we analyzed the seasonal variation in mRNA abundance in cork tissue of selected genes by real time reverse-transcriptase polymerase chain reaction (RT-PCR). Relative transcript abundance was evaluated by principal component analysis and genes were clustered in several functional subgroups. Structural genes of suberin pathways such as CYP86A1, GPAT and HCBT, and regulatory genes of the NAM and WRKY families showed highest transcript accumulation in June, a crucial month for cork development. Other cork structural genes, such as FAT and F5H, were significantly correlated with temperature and relative humidity. The stress genes HSP17.4 and ANN were strongly positively correlated to temperature, in accord with their protective role.
authors.affiliation: Laboratori del Suro, Department of Biology, Universitat de Girona, Campus Montilivi s/n, Girona, Spain.
authors.forename: Marçal
authors.initials: M
authors.lastname: Soler
authors.forename: Olga
authors.initials: O
authors.lastname: Serra
authors.forename: Marisa
authors.initials: M
authors.lastname: Molinas
authors.forename: Emili
authors.initials: E
authors.lastname: García-Berthou
authors.forename: Antònia
authors.initials: A
authors.lastname: Caritat
authors.forename: Mercè
authors.initials: M
authors.lastname: Figueras
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D029963:Quercus / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012621:Seasons
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger