title: Food-associated lactic acid bacteria with antimicrobial potential from traditional Mexican foods.
abstract: This work was conducted to identify indigenous LAB capable of antimicrobial activity, present in traditional Mexican-foods with potential as natural preservatives. A total of 27 artisan unlabeled Mexican products were evaluated, from which 94 LAB strains were isolated, and only 25 strains showed antimicrobial activity against at least one pathogen indicator microorganism. Most of the inhibitory activity showed by the isolated LAB strains was attributed to pH reduction by organic acids. Lactobacillus and Lactococcus strains were good acid producers, depending on the substrate, and may enhance the safety of food products. Cell free cultures of Leuconostoc mesenteroides CH210, and PT8 (from chorizo and pulque, respectively) reduced the number of viable cells of enteropathogenic E. coli in broth system. Lb. plantarum CC10 (from "madre" of vinegar) showed significant inhibitory effect against S. aureus 8943. E. faecium QPII (from panela cheese) produced a bacteriocin with wide anti-L. monocytogenes activity. Selected LAB from traditional Mexican foods showed good potential as bio-preservatives.
authors.affiliation: Departamento de Investigación y Postgrado en Alimentos, PROPAC, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario s/n, Col. Las Campanas, México.
authors.forename: C
authors.initials: C
authors.lastname: Alvarado
authors.forename: B E
authors.initials: BE
authors.lastname: García Almendárez
authors.forename: S E
authors.initials: SE
authors.lastname: Martin
authors.forename: C
authors.initials: C
authors.lastname: Regalado
mesh_terms: D000143:Acids / Q000378:metabolism*; D000434:Alcoholic Beverages / Q000382:microbiology; D000890:Anti-Infective Agents / Q000378:metabolism*; D001430:Bacteriocins / Q000378:metabolism*; D002611:Cheese / Q000382:microbiology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D005519:Food Preservation; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D007778:Lactobacillus / Q000302:isolation & purification* / Q000378:metabolism; D017045:Lactococcus / Q000302:isolation & purification* / Q000378:metabolism; D007934:Leuconostoc / Q000302:isolation & purification* / Q000378:metabolism; D008461:Meat Products / Q000382:microbiology; D008800:Mexico; D008826:Microbial Sensitivity Tests
chemical_list: D000143:Acids; D000890:Anti-Infective Agents; D001430:Bacteriocins; D017077:Culture Media, Conditioned