Effects of continuous elevated cortisol concentrations during oestrus on concentrations and patterns of progesterone, oestradiol and LH in the sow.
This study investigated the effect of continuous elevated cortisol concentrations during standing oestrus on time of ovulation and patterns of progesterone, oestradiol and luteinising hormone (LH) in sows. The elevation of cortisol concentrations was achieved through repeated intravenous injections of synthetic adrenocorticotropic hormone (ACTH) every 2 h for approximately 48 h, from the onset of the second standing oestrus after weaning. Treatment was terminated when ovulation was detected (monitored by transrectal ultrasonography every 4h) or when the sow had received a maximum of 24 injections. The dose of ACTH (2.5 microg/kg) was chosen to mimic the cortisol concentrations seen during mixing of unfamiliar sows. The sows (n=14) were surgically fitted with jugular vein catheters and randomly divided into a control (C group where only NaCl solution were injected) or an ACTH group. Blood samples were collected every 2 h. In parallel with the blood sampling, saliva samples for cortisol analyses were taken from eight sows before onset of treatment and from four of the sows during treatment. There was no difference in time from onset of standing oestrus to ovulation between the two groups. The interval between the peaks of oestradiol and LH to ovulation was prolonged in the ACTH group compared to the C group (p<0.05), with a tendency towards an earlier decline of oestradiol in the ACTH group. Cortisol and progesterone concentrations were significantly elevated during treatment in the ACTH group (p<0.001), with cortisol peak concentrations occurring between 40 and 80 min after each ACTH injection. Cortisol concentrations in saliva and plasma were highly correlated (p<0.001). In conclusion, elevated cortisol concentrations from the onset of standing oestrus increase progesterone concentrations and prolong the interval between oestradiol and LH peaks to ovulation, the latter possible due to an early decline in oestradiol concentrations and a change of the LH peak outline. The effect these hormonal changes have on reproductive performance need to be further investigated. Saliva samples might be a useful and non-invasive method to assess cortisol concentrations in sows.
Brandt
Y
Y
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences (SLU), P.O. Box 7054, SE-750 07 Uppsala, Sweden. ylva.brandt@kv.slu.se
Einarsson
S
S
Ljung
A
A
Lundeheim
N
N
Rodríguez-Martínez
H
H
Madej
A
A
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D004958:Estradiol / Q000097:blood*; D004971:Estrus / Q000502:physiology*; D005260:Female; D006854:Hydrocortisone / Q000097:blood / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood*; D010060:Ovulation / Q000187:drug effects; D011374:Progesterone / Q000097:blood*; D011897:Random Allocation; D012463:Saliva / Q000378:metabolism; D018709:Statistics, Nonparametric; D013312:Stress, Physiological; D013552:Swine / Q000378:metabolism*
D011374:Progesterone; D004958:Estradiol; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D006854:Hydrocortisone