title: [Diagnostic value for detection of aspergillus ribosomal RNA in fungal rhinosinusitis with hybridization in situ].
abstract: OBJECTIVE
  To develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis.
  
  
  METHODS
  Thirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementary to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species.
  
  
  RESULTS
  In situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23).
  
  
  CONCLUSIONS
  In situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed.
authors.affiliation: Department of Otorhinolaryngology, Beijing China-Japan Friendship Hospital, Beijing 100029, China. yuanli0702@263.net
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Na-Ya
authors.initials: NY
authors.lastname: Wang
authors.forename: Da-Zhang
authors.initials: DZ
authors.lastname: Yang
authors.forename: Qiu-Hang
authors.initials: QH
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
mesh_terms: D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000175:diagnosis*; D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D012331:RNA, Fungal / Q000302:isolation & purification; D012337:RNA, Ribosomal, 18S / Q000302:isolation & purification; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S