[Separation, purification and spectrum analysis of total flavonoids from Cercis chinensis].
OBJECTIVE
To separate, purify total flavonoids from Cercis chinensis Bge and analyze their flavonoid aglycones and content by spectrum method.


METHODS
The total flavonoids from Cercis chinensis Bge were extracted by 15 volume of 55% ethyl alcohol and hot water refluence at 100 degrees C and separated, purified by polyamide resin chromatography. Based on reference substances of rutin, quercetin, kaempferol, it was analyzed and detected by UV-Vis spectroscope at 200-600 nm and by KBr infrared spectroscope at 400-4000 cm(-1).


RESULTS
Average extraction rate of crude total flavonoids was 7.32% , and extraction rate of purified total flavonoids was 2.20%. The results of UV-Vis and IR scanning spectrum showed that purified total flavonoids from Cercis chinensis Bge contained of 3 kinds of flavonoid aglycones of rutin, quercetin, kaempferol, and their content was 0.8271, 0.2169, 0.3007 mg/ml respectively.


CONCLUSIONS
Content of total flavonoid aglycones in purified flavonoids of Cercis chinensis Bge was 24.30%, and content of total flavonoid was 0.5346%.
Xu
Mei-yi
MY
Analysis Center, Guangdong Medical College, Zhangjiang 524023, China. myxugdmc@126.com
Han
Ya-li
YL
Dong Ye
Guang-zhi
GZ
Zhang
Li-jian
LJ
D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry; D044949:Kaempferols / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000032:analysis; D012431:Rutin / Q000032:analysis; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D005419:Flavonoids; D044949:Kaempferols; D012431:Rutin; C006552:kaempferol; D011794:Quercetin