Effects of IL-17 on human gingival fibroblasts.
Interleukin (IL)-17 is present in inflammatory periodontal lesions, thus suggesting a role in mediating inflammation. We tested the hypothesis that IL-17, especially when combined with interferon (IFN)-gamma, may modulate the responses of human gingival fibroblasts (HGFs). IL-17 induced IL-8 and minimal intercellular adhesion molecule (ICAM)-1 expression. It had no effect on expression of HLA-DR, CD40, or the immune-suppressive enzyme indoleamine 2,3-dioxygenase (IDO). The effects of IL-17 on HGFs were compared with those of IFN-gamma. Unlike IL-17, IFN-gamma augmented the expression of HLA-DR, ICAM-1, and IDO, but not IL-8. Thus, IL-17 and IFN-gamma induce different HGF responses when administered separately. Interestingly, when IL-17 and IFN-gamma were combined, marked enhancement of ICAM-1, IL-8, and IDO expression by HGFs was observed. These findings suggest that IL-17, especially when combined with IFN-gamma, could play an important role in immune modulation through stimulation of HGFs in periodontal disease.
Mahanonda
R
R
Department of Periodontology, Faculty of Dentistry, University, Henri Dunant Rd., Bangkok 10330, Thailand. mrangsin@chula.ac.th
Jitprasertwong
P
P
Sa-Ard-Iam
N
N
Rerkyen
P
P
Charatkulangkun
O
O
Jansisyanont
P
P
Nisapakultorn
K
K
Yongvanichit
K
K
Pichyangkul
S
S
D019013:CD40 Antigens / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects*; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006684:HLA-DR Antigens / Q000187:drug effects; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D020381:Interleukin-17 / Q000494:pharmacology*; D016209:Interleukin-8 / Q000187:drug effects
D019013:CD40 Antigens; D006684:HLA-DR Antigens; D007155:Immunologic Factors; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D020381:Interleukin-17; D016209:Interleukin-8; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma