Interaction between Pazufloxacin and DNA mediated by copper(II) ions.
The influence of copper(II) ions on the binding of Pazufloxacin (PFLX), which is a forth generation quinolone antimicrobial agent, to calf thymus DNA (CTDNA) has been investigated by fluorescence, absorption spectroscopy and viscosity measurement. The results show that PFLX and Cu(2+) can form binary complex with mole ratio 2:1, PFLX and CTDNA can form complex mediated by Cu(2+). A ternary system of PFLX-Cu(2+)-DNA were proposed. The experimental results show that the PFLX binds to CTDNA by two kinds of binding modes, intercalative mode and groove binding mode.
Zhang
Guoliang
G
College of Science, Zhejiang University, Hangzhou, People's Republic of China.
Fu
Xuchun
X
Liu
Qingwang
Q
Wang
Guoping
G
D000995:Antitubercular Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D024841:Fluoroquinolones / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
D000995:Antitubercular Agents; D024841:Fluoroquinolones; D010078:Oxazines; C078052:pazufloxacin; D003300:Copper; D004247:DNA; C427203:calf thymus DNA