title: [Expression of Omi/HtrA2 UP-regulated in the spermatozoa of chronic bacterial prostatitis patients].
abstract: OBJECTIVE
  To determine the expression of Omi/HtrA2 in the spermatozoa of chronic bacterial prostatitis patients and explore the potential mechanism of chronic prostatitis inducing male infertility.
  
  
  METHODS
  Forty-one patients diagnosed as suffering from chronic prostatitis were included, and so were 12 healthy normal men as controls. Spermatozoa in the semen were purified by Percoll gradient technique to separate the seminal plasma and other round cells. Sperm concentration, motility, morphology, pro-inflammatory cytokines, Omi/HtrA2 mRNA and protein levels in the spermatozoa of the patients were measured.
  
  
  RESULTS
  Significantly increased levels of pro-inflammatory cytokines were observed in the seminal plasma of the prostatitis patients, and so were Omi/HtrA2 mRNA and protein levels as compared with the normal men.
  
  
  CONCLUSIONS
  Chronic prostatitis patients presented important alterations in semen quality parameters and up-regulated expression of Omi/HtrA2 mRNA and proteins in the spermatozoa.
authors.affiliation: Department of Urology, 6th People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China. jd6hyong@yahoo.com
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Hu
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Qiao
authors.forename: Zhang-Shun
authors.initials: ZS
authors.lastname: Liu
authors.forename: Lu-Jie
authors.initials: LJ
authors.lastname: Song
authors.forename: Chao
authors.initials: C
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D015870:Gene Expression; D000074923:High-Temperature Requirement A Serine Peptidase 2; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D011472:Prostatitis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D053583:Interleukin-1beta; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D012697:Serine Endopeptidases; C000615914:HTRA2 protein, human; D000074923:High-Temperature Requirement A Serine Peptidase 2