title: Properties of TiO2-polyacrylic acid dispersions with potential for molecular recognition.
abstract: Titanium dioxide (TiO2)/polyacrylic acid (PAA) (TiO2/PAA) particles were formed by mixing PAA and an acidic solution of TiO2 nanoparticles in dimethylformamide (DMF) followed by heat treatment. TEM and particle analysis showed that the resulting particles had a narrow size distribution. The colloid was very stable and aggregation was not observed over a wide pH range (3-9) or at high salt concentration. The residual carboxylic acid of PAA could be modified via EDC/NHS activation to form an amide bond with a protein. An antibody was attached to the hybrid nanoparticle and specific binding to antigen was monitored by surface plasmon resonance. The results suggest that TiO2/PAA nanoparticles are candidates as the base component of a photocatalytic system with potential for substrate selectivity.
authors.affiliation: TOTO Ltd. Research Institute, Biotechnology Group, Nakashima 2-1-1, Kokurakita, Kitakyushu, Fukuoka 802-8601, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Kanehira
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Banzai
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimizu
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kubota
authors.forename: Shuji
authors.initials: S
authors.lastname: Sonezaki
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry* / Q000378:metabolism; D001666:Binding Sites, Antibody; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D020349:Surface Plasmon Resonance; D014025:Titanium / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; C009495:titanium dioxide; C006903:carbopol 940; D014025:Titanium