title: Separation and quantitation of several angiotensin II receptor antagonist drugs in human urine by a SPE-HPLC-DAD method.
abstract: In this work, an SPE-HPLC method coupled to photodiode array detection was validated in human urine matrix, in order to monitor four antihypertensive angiotensin II receptor antagonist drugs in patients under cardiovascular treatment. For that purpose, experimental design was used. Quantitation was accomplished by the internal standard method. The obtained LOQs were 95, 113, 125, and 85 ng/mL for eprosartan, telmisartan, irbesartan, and valsartan, respectively. The intraday and interday precision and accuracy at four concentration levels in the working range (LOQ-15 microg/mL) were always lower than 11% RSD and 8% relative error. The urine samples proved to be stable during 4 h at room temperature, after three thaw-freeze cycles, and for 2 months at -20 degrees C. No interferences from other endogenous compounds or co-administered drugs were found. The method has been successfully applied to monitor the renal elimination of eprosartan and valsartan during 24 h.
authors.affiliation: Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Leioa, Spain.
authors.forename: Nerea
authors.initials: N
authors.lastname: Ferreirós
authors.forename: Gorka
authors.initials: G
authors.lastname: Iriarte
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Alonso
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Jiménez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ortíz
mesh_terms: D000179:Acrylates / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D000368:Aged; D000369:Aged, 80 and over; D047228:Angiotensin II Type 1 Receptor Blockers / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*; D057911:Angiotensin Receptor Antagonists; D000959:Antihypertensive Agents / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*; D001562:Benzimidazoles / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D001565:Benzoates / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D001713:Biphenyl Compounds / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D000077405:Irbesartan; D008297:Male; D008875:Middle Aged; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D000077333:Telmisartan; D013777:Tetrazoles / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D013876:Thiophenes / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D014633:Valine / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification / Q000652:urine; D000068756:Valsartan
chemical_list: D000179:Acrylates; D047228:Angiotensin II Type 1 Receptor Blockers; D057911:Angiotensin Receptor Antagonists; D000959:Antihypertensive Agents; D001562:Benzimidazoles; D001565:Benzoates; D001713:Biphenyl Compounds; D007093:Imidazoles; D013777:Tetrazoles; D013876:Thiophenes; C068373:eprosartan; D000068756:Valsartan; D014633:Valine; D000077405:Irbesartan; D000077333:Telmisartan