title: UTILLdb, a Pisum sativum in silico forward and reverse genetics tool.
abstract: The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.
authors.affiliation: Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Rue Gaston Crémieux, 91057 Evry Cedex, France. dalmais@evry.inra.fr
authors.forename: Marion
authors.initials: M
authors.lastname: Dalmais
authors.forename: Julien
authors.initials: J
authors.lastname: Schmidt
authors.forename: Christine
authors.initials: C
authors.lastname: Le Signor
authors.forename: Francoise
authors.initials: F
authors.lastname: Moussy
authors.forename: Judith
authors.initials: J
authors.lastname: Burstin
authors.forename: Vincent
authors.initials: V
authors.lastname: Savois
authors.forename: Gregoire
authors.initials: G
authors.lastname: Aubert
authors.forename: Veronique
authors.initials: V
authors.lastname: Brunaud
authors.forename: Yannick
authors.initials: Y
authors.lastname: de Oliveira
authors.forename: Cecile
authors.initials: C
authors.lastname: Guichard
authors.forename: Richard
authors.initials: R
authors.lastname: Thompson
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
mesh_terms: D000483:Alleles; D004252:DNA Mutational Analysis / Q000379:methods*; D030541:Databases, Genetic; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D017343:Genes, Plant; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D018532:Pisum sativum / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics*
chemical_list: D009153:Mutagens; D005020:Ethyl Methanesulfonate