The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic.
We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1.
Mok
Young Geun
YG
Department of Molecular Biology, Sejong University, 98 Kunja-Dong, Kwangjin-Gu, Seoul, Republic of Korea.
Lee
Byoung Doo
BD
Kim
Young Jin
YJ
Lee
Chang Eun
CE
Kim
Dong Gwan
DG
Lee
Joohyun
J
Shim
Jaekyung
J
Meng
Yuling
Y
Rosen
Barry P
BP
Choi
Jong Soon
JS
Shin
Hyoung Sun
HS
Kim
Seong-Ki
SK
Lee
June Seung
JS
Hwang
Seongbin
S
D000595:Amino Acid Sequence; D001151:Arsenic / Q000032:analysis / Q000378:metabolism*; D018053:Arsenites / Q000494:pharmacology*; D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics*; D017343:Genes, Plant; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism
C109281:ACR3 protein, S cerevisiae; D018053:Arsenites; D050976:Basic-Leucine Zipper Transcription Factors; C093263:FPS1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D012097:Repressor Proteins; C077832:SKO1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C015001:arsenite; D001151:Arsenic