SAGE and LongSAGE.
Serial analysis of gene expression (SAGE) is a high-throughput method for global gene expression analysis that allows the quantitative and simultaneous analysis of a large number of transcripts. SAGE is a digital method and its sensitivity depends only on the number of tags sequenced. Furthermore, SAGE is a powerful tool for finding novel genes that are expressed under certain conditions or in certain tissues. SAGE has been widely used in fields as diverse as cancer research and the development and study of microorganisms. The SAGE method is a series of routine molecular biology procedure and can, at least in principle, be carried out in any laboratory. However, the number of consecutive steps is quite large and in practice, SAGE has been difficult to carry out on a routine basis.
Høgh
Annabeth Laursen
AL
Department of Biochemistry, Chemistry and Environmental Engineering, University of Aalborg, Aalborg, Denmark.
Nielsen
Kåre Lehmann
KL
D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000235:genetics; D014170:Transformation, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D018749:RNA, Plant; C049760:endodeoxyribonuclease MmeI; C048422:endodeoxyribonuclease NlaIII; D015252:Deoxyribonucleases, Type II Site-Specific