A novel application of ratio spectra derivative spectroscopy for the determination of amphotericin in highly icteric plasma.
Derivative spectroscopy has been utilised for the determination of amphotericin in various biological matrices including plasma, serum, urine and brain tissue. Whilst these methods have all been shown to be suitable for the determination of the drug in these matrices it has been reported that the application fails in the case of highly icteric plasma, this being due to the presence of high concentrations (>50microM) of bilirubin. This paper details the application of ratio spectra derivative spectroscopy to overcome the interference of bilirubin with amphotericin in such situations.
Millership
Jeffrey Stuart
JS
Children's Medicines Research Group, School of Pharmacy, Queen's University Belfast, Medical Biology Centre, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, United Kingdom. j.millership@qub.ac.uk
McCaffrey
Fintan
F
Tierney
Dara
D
D000666:Amphotericin B / Q000097:blood*; D001663:Bilirubin / Q000097:blood; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000666:Amphotericin B; D001663:Bilirubin