title: Peptidergic influences on proliferation, migration, and placement of neural progenitors in the adult mouse forebrain.
abstract: Neural progenitor proliferation, differentiation, and migration are continually ongoing processes in the subventricular zone (SVZ) and rostral migratory stream (RMS) of the adult brain. There is evidence that peptidergic systems may be involved in the molecular cascades regulating these neurogenic processes, and we examined a possible influence of neuropeptide Y (NPY) and cholecystokinin (CCK) systems in cell proliferation and neuroblast formation in the SVZ and RMS and generation of interneurons in the olfactory bulb (OB). We show that NPY and the Y1 and Y2 receptor (R) proteins are expressed in and surrounding the SVZ and RMS and that Y1R is located on neuroblasts in the anterior RMS. Mice deficient in Y1Rs or Y2Rs have fewer Ki-67-immunoreactive (ir) proliferating precursor cells and doublecortin-ir neuroblasts in the SVZ and RMS than WT mice, and less calbindin-, calretinin-, and tyrosine hydroxylase-ir interneurons in the OB. Mice lacking CCK1Rs have fewer proliferating cells and neuroblasts than normal and a shortage of interneurons in the OB. These findings suggest that both NPY and CCK through their receptors help to regulate the proliferation of precursor cells, the amount of neuroblast cells in the SVZ and RMS, and influence the differentiation of OB interneurons.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, S-17177 Stockholm, Sweden. davor.stanic@florey.edu.au
authors.forename: Davor
authors.initials: D
authors.lastname: Stanic
authors.forename: Gustavo
authors.initials: G
authors.lastname: Paratcha
authors.forename: Fernanda
authors.initials: F
authors.lastname: Ledda
authors.forename: Herbert
authors.initials: H
authors.lastname: Herzog
authors.forename: Alan S
authors.initials: AS
authors.lastname: Kopin
authors.forename: Tomas
authors.initials: T
authors.lastname: Hökfelt
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D007395:Interneurons; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000502:physiology*; D009830:Olfactory Bulb; D016548:Prosencephalon / Q000166:cytology*; D011949:Receptors, Cholecystokinin / Q000502:physiology*; D017476:Receptors, Neuropeptide Y / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D009479:Neuropeptides; D011949:Receptors, Cholecystokinin; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C095889:neuropeptide Y2 receptor