High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.
We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.
Spurgeon
Sandra L
SL
Fluidigm Corporation, South San Francisco, California, USA.
Jones
Robert C
RC
Ramakrishnan
Ramesh
R
D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D016133:Polymerase Chain Reaction