title: Comparison of the effectiveness of autologous fibrin glue and macroporous biphasic calcium phosphate as carriers in the osteogenesis process with or without mesenchymal stem cells.
abstract: BACKGROUND
  Facial bone reconstruction has been a challenge for oral and maxillofacial surgeons for a long time. Recently, some studies have reported the use of stem cells in facial reconstruction to achieve osteogenesis. However, to ensure that stem cells remain in the recipient site, a biocompatible carrier is needed to transfer the stem cells. Fibrin glue has been shown to promote hemostasis in wound management and accelerate soft tissue healing, but the role of fibrin glue in bone regeneration remains debatable. The purpose of this study was to compare the effectiveness of autologous fibrin glue and macroporous biphasic calcium phosphate (MBCP) as carriers in the osteogenesis process with/without mesenchymal stem cells.
  
  
  METHODS
  Fifteen New Zealand white rabbits were used in this study. Mesenchymal stem cells were harvested from the iliac bone, and autologous fibrin glue was made from peripheral blood. Three cranial defects with a diameter of 6 mm were created over the cranial bone in each rabbit. The 15 animals were separated into 2 groups. The first group contained 12 rabbits. The grafted substances placed over the regions of defect were: (1) stem cells plus autologous fibrin glue; (2) stem cells plus MBCP; (3) defect alone as control. In the second group of 3 rabbits, the cranial defects were grafted with: (1) autologous fibrin glue alone; (2) MBCP alone; (3) defect alone as control. Rabbits were sacrificed at 1, 2 and 3 months post operation. Radiography and histology were used to detect bone formation.
  
  
  RESULTS
  Stem cells plus autologous fibrin glue induced more bone formation 2 months post operation and more mature bone was found 3 months post operation compared with the other groups. MBCP with or without stem cells showed moderate tissue reaction, including giant cell, histiocyte and eosinophil cell accumulation.
  
  
  CONCLUSIONS
  Using stem cells plus autologous fibrin glue as the carrier may accelerate new bone regeneration.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Taichung Veterans General Hospital, Taichung, Taiwan, ROC.
authors.forename: Li-Tzu
authors.initials: LT
authors.lastname: Lee
authors.forename: Po-Cheung
authors.initials: PC
authors.lastname: Kwan
authors.forename: Ya-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Yong-Kie
authors.initials: YK
authors.lastname: Wong
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000008:administration & dosage*; D015718:Fibrin Tissue Adhesive / Q000008:administration & dosage*; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D010012:Osteogenesis; D014677:Pharmaceutical Vehicles; D016062:Porosity; D011817:Rabbits; D011859:Radiography; D012886:Skull / Q000000981:diagnostic imaging / Q000601:surgery; D023822:Tissue Engineering
chemical_list: D002130:Calcium Phosphates; D015718:Fibrin Tissue Adhesive; D014677:Pharmaceutical Vehicles; C020243:calcium phosphate