title: The biofilm exopolysaccharide polysaccharide intercellular adhesin--a molecular and biochemical approach.
abstract: Exopolysaccharides play a crucial role in the formation of biofilms and biofilm resistance to antimicrobials and innate host defense. Here we describe methods to analyze and quantify polysaccharide intercellular adhesin (PIA), a biofilm exopolysaccharide made of N-acetylglucosamine that is found in staphylococci and many other bacterial biofilm-forming pathogens.
authors.affiliation: Department of Anesthesiology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Cuong
authors.initials: C
authors.lastname: Vuong
authors.forename: Michael
authors.initials: M
authors.lastname: Otto
mesh_terms: D018441:Biofilms; D007118:Immunoassay; D008856:Microscopy, Fluorescence; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D013210:Staphylococcus / Q000235:genetics / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; C098632:exopolysaccharide, Streptococcus; C113579:polysaccharide intercellular adhesin