Hyperthermic treatment of DMBA-induced rat mammary cancer using magnetic nanoparticles.
BACKGROUND
We have developed magnetite cationic liposomes (MCLs) and applied them as a mediator of local hyperthermia. MCLs can generate heat under an alternating magnetic field (AMF). In this study, the in vivo effect of hyperthermia mediated by MCLs was examined using 7,12-dimethylbenz(a)anthracene (DMBA)-induced rat mammary cancer as a spontaneous cancer model.


METHODS
MCLs were injected into the mammary cancer and then subjected to an AMF.


RESULTS
Four rats in 20 developed mammary tumors at more than 1 site in the body. The first-developed tumor in each of these 4 rats was selected and heated to over 43 degrees C following administration of MCLs by an infusion pump. After a series of 3 hyperthermia treatments, treated tumors in 3 of the 4 rats were well controlled over a 30-day observation period. One of the 4 rats exhibited regrowth after 2 weeks. In this rat, there were 3 sites of tumor regrowth. Two of these regrowths were reduced in volume and regressed completely after 31 days, although the remaining one grew rapidly. These results indicated hyperthermia-induced immunological antitumor activity mediated by the MCLs.


CONCLUSIONS
Our results suggest that hyperthermic treatment using MCLs is effective in a spontaneous cancer model.
Motoyama
Jun
J
1Nanotherapy Co, Ltd, 19-11, Kikui 2-chome, Nishi-ku, Nagoya 451-0044, Japan. jun.motoyama@nipponkayaku.co.jp
Yamashita
Noriyuki
N
Morino
Tomio
T
Tanaka
Masashi
M
Kobayashi
Takeshi
T
Honda
Hiroyuki
H