title: [Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of reducing N2O, isolated from soda lakes].
abstract: In the course of the search for N2O-utilizing microorganisms, two novel strains of haloalkaliphilic denitrifying bacteria, Z-7009 and AIR-2, were isolated from soda lakes of Mongolia and Kenya. These microorganisms are true alkaliphiles and grow in the pH ranges of 8.0-10.5 and 7.5-10.6, respectively. They are facultative anaerobes with an oxidative type of metabolism, able to utilize a wide range of organic substrates and reduce nitrate, nitrous oxide, and, to a lesser extent, nitrite to gaseous nitrogen. They can oxidize sulfide in the presence of acetate as the carbon source and nitrous oxide (strain Z-7009) or nitrate (strain AIR-2) as the electron acceptor. The strains require Na+ ions. They grow at medium mineralization levels of 0.16-2.2 M Na+ (Z-7009) and 0.04-2.2 M Na+ (AIR-2). The G+C contents of the DNA of strains Z-7009 and AIR-2 are 67.9 and 65.5 mol %, respectively. According to the results of 16S rRNA gene sequencing and DNA-DNA hybridization, as well as on the basis of physiological properties, the strains were classified as new species of the genus Halomonas: Halomonas mongoliensis, with the type strain Z-7009T (=DSM 17332, =VKM B2353), and Halomonas kenyensis, with the type strain AIR-2T (=DSM 17331, =VKM B2354).
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Boltianskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Kevbrin
authors.forename: A M
authors.initials: AM
authors.lastname: Lysenko
authors.forename: T V
authors.initials: TV
authors.lastname: Kolganova
authors.forename: T P
authors.initials: TP
authors.lastname: Turova
authors.forename: G A
authors.initials: GA
authors.lastname: Osipov
authors.forename: T N
authors.initials: TN
authors.lastname: Zhilina
mesh_terms: D001482:Base Composition; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D005740:Gases / Q000378:metabolism; D020632:Halomonas / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007630:Kenya; D008986:Mongolia; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D005740:Gases; D009566:Nitrates; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D009609:Nitrous Oxide; D009584:Nitrogen