title: Identification of an NADH-dependent 5-hydroxymethylfurfural-reducing alcohol dehydrogenase in Saccharomyces cerevisiae.
abstract: We report on the identification and characterization of a mutated alcohol dehydrogenase 1 from the industrial Saccharomyces cerevisiae strain TMB3000 that mediates the NADH-dependent reduction of 5-hydroxymethylfurfural (HMF) to 2,5-bis-hydroxymethylfuran. The co-factor preference distinguished this alcohol dehydrogenase from the previously reported NADPH-dependent S. cerevisiae HMF alcohol dehydrogenase Adh6. The amino acid sequence revealed three novel mutations (S109P, L116S and Y294C) that were all predicted at the vicinity of the substrate binding site, which could explain the unusual substrate specificity. Increased biomass production and HMF conversion rate were achieved in a CEN.PK S. cerevisiae strain overexpressing the mutated ADH1 gene.
authors.affiliation: Department of Applied Microbiology, Lund University, PO Box 124, SE-22100 Lund, Sweden.
authors.forename: Boaz
authors.initials: B
authors.lastname: Laadan
authors.forename: João R M
authors.initials: JR
authors.lastname: Almeida
authors.forename: Peter
authors.initials: P
authors.lastname: Rådström
authors.forename: Bärbel
authors.initials: B
authors.lastname: Hahn-Hägerdal
authors.forename: Marie
authors.initials: M
authors.lastname: Gorwa-Grauslund
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003067:Coenzymes / Q000378:metabolism; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D013058:Mass Spectrometry; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D003067:Coenzymes; D029701:Saccharomyces cerevisiae Proteins; D009243:NAD; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; C516801:ADH1 protein, S cerevisiae; D000426:Alcohol Dehydrogenase