Ultrasonic approach to enhance signal-to-noise in three dimensional microarray.
Three-dimensional polyacrylamide gel microarray (3-D gel microarray) has a strong adsorption for hybridzation probes with fluorescent label, resulting in high fluorescent background noise. The background noise has hampered the broad application of the microarray in scientific research. A low-frequency ultrasound of 40 kHz has been successfully employed to efficiently remove the nonspecifically bound fluorescent probes from the 3-D gel microarray. The method can significantly enhance the signal to noise ratio of the 3-D gel microarray and make it work better in single-nucleotide polymorphism genotyping.
Pan
Zhiqiang
Z
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
Li
Yanqiang
Y
Zhou
Dongrui
D
Tang
Jing
J
Zhang
Minghao
M
Xiao
Pengfeng
P
Lu
Zuhong
Z
D000180:Acrylic Resins; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results; D014465:Ultrasonics
D000180:Acrylic Resins; C016680:polyacrylamide gels