title: Esterases activities and lipid peroxidation levels in muscle tissue of the shanny Lipophrys pholis along several sites from the Portuguese Coast.
abstract: This study is part of a project aiming to validate the use of the intertidal shanny Lipophorys pholis as a sentinel species in pollution monitoring in NW European marine ecosystems. To this end, a characterisation of acethylcholin (AChE), butyrylcholin (BChE) and propionylcholin (PrChE) esterases in L. pholis muscle was performed and the results indicated that AChE was predominant. Furthermore, the use of eserine sulphate and BW284c51 (0.64-800 microM), and iso-OMPA (0.08-16 mM), confirmed the measurement of true cholinesterases (ChEs) as well as the presence of pseudocholinesterases. The field application of these markers to L. pholis, sampled in seven locations along the Portuguese coast, revealed that fish were likely to be affected by neurotoxic compounds. This was indicated by the significant depletion of AChE (p<0.05) in animals collected at urban and industrialised sites, compared with those from reference locations. The inclusion of a marker of effect, measured as lipid peroxidation levels in muscle tissue, also revealed the existence of site differences. Overall, the study further validates the utility of L. pholis in pollution monitoring studies.
authors.affiliation: Institut de Ciències del Mar ICM-CSIC, Pg. Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain. msole@icm.csic.es
authors.forename: Montserrat
authors.initials: M
authors.lastname: Solé
authors.forename: Gemma
authors.initials: G
authors.lastname: Lobera
authors.forename: Daniela
authors.initials: D
authors.lastname: Lima
authors.forename: Maria Armanda
authors.initials: MA
authors.lastname: Reis-Henriques
authors.forename: Miguel Machado
authors.initials: MM
authors.lastname: Santos
mesh_terms: D000818:Animals; D015415:Biomarkers; D002802:Cholinesterases / Q000378:metabolism*; D004784:Environmental Monitoring; D005260:Female; D015227:Lipid Peroxidation; D008297:Male; D019216:Metals, Heavy / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D018384:Oxidative Stress; D010473:Perciformes / Q000378:metabolism*; D011174:Portugal; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D015415:Biomarkers; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D002802:Cholinesterases