IkappaB, NF-kappaB regulation model: simulation analysis of small number of molecules.
The regulation of IkappaB, NF-kappaB is of foremost interest in biology as the transcription factor NF-kappaB has multiple target genes. We have modeled a previously published model by Hoffmann et al. (2002) of IkappaB, NF-kappaB mathematically as discrete reaction systems. We have used stochastic algorithm to compare the results when there are large and small numbers of molecules available in a finite volume for each protein. Our results for small number of molecules show that with continuous presence of stimulation, nuclear NF-kappaB oscillates continuously in every individual cell rather than damping, which was observed in cell population results. This characteristic of the system is missed when averaged behavior is studied.
Sarkar
Anamika
A
Bioengineering Department, Cell Systems Initiative, University of Washington, Seattle, WA 98195, USA.
Meila
Marina
M
Franza
Robert B
RB