Involvement of calcium-sensing receptor in cardiac hypertrophy-induced by angiotensinII through calcineurin pathway in cultured neonatal rat cardiomyocytes.
Cardiac hypertrophy is a common pathological change accompanying cardiovascular disease. Recently, some evidence indicated that calcium-sensing receptor (CaSR) expressed in the cardiovascular tissue. However, the functional involvement of CaSR in cardiac hypertrophy remains unclear. Previous studies have shown that CaSR caused accumulation of inositol phosphate to increase the release of intracellular calcium. Moreover, Ca(2+)-dependent phosphatase calcineurin (CaN) played a vital role in the development of cardiac hypertrophy. Therefore, we investigated the expression of CaSR in cardiac hypertrophy-induced by angiotensin II (AngII) and the effects of CaSR activated by GdCl(3) on the related signaling transduction pathways. The results showed that AngII induced cardiac hypertrophy and up-regulated the expression of CaSR, meanwhile increased the intracellular calcium concentration ([Ca(2+)](i)) and activated CaN hypertrophic signaling pathway. Compared with AngII alone, the above changes were further obvious when adding GdCl(3). But the effects of GdCl(3) on the cardiac hypertrophy were attenuated by CsA, a specific inhibitor of CaN. In conclusion, these results suggest that CaSR is involved in cardiac hypertrophy-induced by AngII through CaN pathway in cultured neonatal rat cardiomyocytes.
Wang
Li-Na
LN
Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
Wang
Chao
C
Lin
Yan
Y
Xi
Yu-Hui
YH
Zhang
Wei-Hua
WH
Zhao
Ya-Jun
YJ
Li
Hong-Zhu
HZ
Tian
Ye
Y
Lv
Yan-Jie
YJ
Yang
Bao-Feng
BF
Xu
Chang-Qing
CQ
D000804:Angiotensin II; D000818:Animals; D000831:Animals, Newborn; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006332:Cardiomegaly / Q000139:chemically induced* / Q000378:metabolism*; D002478:Cells, Cultured; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000378:metabolism*; D015398:Signal Transduction
D044169:Receptors, Calcium-Sensing; D000804:Angiotensin II; D019703:Calcineurin; D002118:Calcium