Whole genome amplification using single-primer PCR.
Comprehensive genomic molecular analyses require relatively large DNA amounts that are often not available from forensic, clinical and other crucial biological samples. Numerous methods to amplify the whole genome have been proposed for cancer, forensic and taxonomic research. Unfortunately, when using truly random primers for the initial priming step, all of these procedures suffer from high background problems for sub-nanogram quantities of input DNA. Here we report an approach to eliminate this problem for PCR-based methods even at levels of DNA approaching that of a single cell.
Lao
Kaiqin
K
Applied Biosystems, Foster City, CA 94404, USA.
Xu
Nan Lan
NL
Straus
Neil A
NA
D017931:DNA Primers / Q000235:genetics*; D005784:Gene Amplification / Q000235:genetics*; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers