[Increased expression of extracellular matrix metalloproteinase inducer through interaction between fibroblasts and colonic cancer cells].
OBJECTIVE
To investigate the expression of extracellular matrix metalloproteinase inducer gene (EMMPRIN) regulated by the interactions between fibroblasts and colon cancer cells, and to study its role in the invasion and metastasis of colon cancer.


METHODS
Colon cancer cells (SW480) were co-cultured with fibroblasts (HELF) in RPMI 1640 media for 0, 12, 24 and 48 hours. The expression of EMMPRIN in SW480 cells and HELF cells was documented by RT-PCR and immunocytochemistry.


RESULTS
The mRNA and protein expressions of EMMPRIN in SW480 cells were remarkably increased by the co-culturing with HELF cells. Although without the endogenous expression, HELF cells began to express EMMPRIN in a time-dependent manner after being co-cultured with SW480 cells.


CONCLUSIONS
Intercellular interactions between colon cancer cells and fibroblasts not only up-regulate the EMMPRIN expression in SW480 cells, but also induce its expression in HELF cells. Such interactions may play a crucial role in the invasion and metastasis of colon cancer.
Zhang
You-wei
YW
Department of Pathology, School of Basic Medical Sciences, Southeast University, Nanjing 210009, China.
Deng
Hong
H
Chen
Ping-sheng
PS
Chen
Lu-lu
LL
D051926:Basigin / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008168:Lung / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D051926:Basigin