Elevation of cytosolic calcium level triggers calcium antagonist-induced gingival overgrowth.
Calcium (Ca2+) antagonists induce gingival overgrowth as a side effect but the pathogenic mechanism is still unknown. The Ca2+-channel activator Bay K 8644 elevates intracellular Ca2+ concentration ([Ca2+]i) and enhances the cell proliferation of gingival fibroblasts in a dose-dependent manner. Verapamil, an L-type Ca2+-channel blocker, also elevates [Ca2+]i in gingival fibroblasts, but it has no effect on other fibroblasts such as those of the lung, skin, and muscle. Moreover, verapamil enhances the proliferation of fibroblasts of the gingiva but has no effect on the proliferation of those of other tissues. These findings confirm that [Ca2+]i elevation induces the proliferation of gingival fibroblasts.
Hattori
Toshimi
T
Department of Dental Pharmacology, Matsumoto Dental University, Hirooka-Gohbara, Shiojiri 399-0781, Japan. hattori@po.mdu.ac.jp <hattori@po.mdu.ac.jp>
Wang
Pao-Li
PL
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D002118:Calcium / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D003600:Cytosol / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology; D005456:Fluorescent Dyes; D016257:Fura-2; D005881:Gingiva / Q000166:cytology* / Q000254:growth & development*; D006801:Humans; D014700:Verapamil / Q000494:pharmacology
D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D014700:Verapamil; D002118:Calcium; D016257:Fura-2