title: Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni.
abstract: A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n=23) and chronic (n=30) schistosomiasis, with other parasitoses (n=39) or without parasitic infections (n=100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in kappa concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P=0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.
authors.affiliation: Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 580, Cj das Químicas, Bloco 17, 05508-900, São Paulo, SP, Brazil. edwardjo@cpqrr.fiocruz.br
authors.forename: Edward José
authors.initials: EJ
authors.lastname: de Oliveira
authors.forename: Hermínia Yohko
authors.initials: HY
authors.lastname: Kanamura
authors.forename: Kioko
authors.initials: K
authors.lastname: Takei
authors.forename: Rosario Dominguez Crespo
authors.initials: RD
authors.lastname: Hirata
authors.forename: Luiz Carlos Pedrosa
authors.initials: LC
authors.lastname: Valli
authors.forename: Nga Yen
authors.initials: NY
authors.lastname: Nguyen
authors.forename: Izabel
authors.initials: I
authors.lastname: de Carvalho Rodrigues
authors.forename: Amélia Ribeiro
authors.initials: AR
authors.lastname: de Jesus
authors.forename: Mário Hiroyuki
authors.initials: MH
authors.lastname: Hirata
mesh_terms: D000208:Acute Disease; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D010446:Peptide Fragments / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010446:Peptide Fragments