title: Influence of the N- and C-terminal regions of leu-lys rich antimicrobial peptide on antimicrobial activity.
abstract: P5 (KWKKLLKKPLLKKLLKKL-NH(2)) is an antibacterial 18-mer Leu-Lys rich peptide from CA (1-8)-MA (1-12) hybrid peptide (CA-MA). Here we show that decreasing the net hydrophobicity and charge of CA-MA by deleting Leu- or Lys- of the N- or C-terminal regions of P5 (P10 or P11). The antimicrobial activity of the peptides was measured by their growth inhibitory effect upon S. aureus, B. subtilis, P. aeruginosa, S. typhimurium, E. coli, T. beigelii and C. albicans. Antimicrobial activity required a full length C-terminus. Confocal microscopy showed that P11 was located in the plasma membrane. In this study, P11, K(3)K(4)L(5)L(6)-deleted peptide, acted independent on the ionic environment. Furthermore, P11 causes significant morphological alterations of the fungal surfaces as shown by scanning electron microscopy.
authors.affiliation: Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea.
authors.forename: Hae Kyun
authors.initials: HK
authors.lastname: Park
authors.forename: Hak-Tae
authors.initials: HT
authors.lastname: Lim
authors.forename: Byung Jo
authors.initials: BJ
authors.lastname: Chae
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
authors.forename: Yoonkyung
authors.initials: Y
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects; D007930:Leucine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012492:Salts / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D012492:Salts; D007930:Leucine; D008239:Lysine