[Effect of co-culture of bone marrow mesenchymal stem cells and K562 cells on expression of interleukin 8].
To investigate the effects of interaction between human bone marrow mesenchymal stem cells (MSCs) and K562 cells on the expression of proangiogenic factor IL-8, the K562 cells were co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium while the controlled K562 cells were cultured alone. The IL-8 gene expression of K562 cells and MSCs was determined by RT-PCR. The results indicated that the expression of IL-8 in K562 cells when co-cultured in direct contact with MSCs or cultured in MSCs conditioned medium was obviously higher than that of K562 cells cultured alone (p<0.01). MSCs co-cultured with K562 cells also had an increased level of IL-8 compared with MSCs cultured alone (p<0.01). It is concluded that the interaction between MSCs and K562 cells via direct contact and the cytokine network promotes expression of IL-8.
Wang
Li-Xia
LX
Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Lu
Hua
H
Liu
Peng
P
Fei
Xiao-Ming
XM
Wu
Yu-Jie
YJ
Wang
Cheng-Ya
CY
Li
Jian-Yong
JY
D001854:Bone Marrow Cells / Q000166:cytology*; D018920:Coculture Techniques; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D016209:Interleukin-8; D012333:RNA, Messenger