NMR study of the sorption behavior of benzyl alcohol derivatives into sonicated and extruded dioctadecyldimethylammonium chloride (DODAC) dispersions: the relevance of membrane fluidity.
The sorption behavior of three benzyl alcohol derivatives with different hydrophobicities into sonicated and extruded DODAC dispersions has been studied using NMR spectroscopy and NMR diffusometry. We show that there is an increased sorption into a sonicated dispersion below the phase-transition temperature (T(m)) as compared to an extruded dispersion. This may be explained by the incomplete lipid chain freezing of charged lipids as a result of the sonication process. Around T(m), a sorption maximum is found that is attributed to the high bilayer disorder under this condition. In addition, a sorption increase and a fluidizing effect at increasing benzyl alcohol derivative concentrations are observed that provide additional evidence for the relevance of the bilayer fluidity on the sorption of hydrophobic components.
Saveyn
Pieter
P
Particle and Interfacial Technology Group, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Gent, Belgium. pieter.saveyn@ugent.be
Cocquyt
Ellen
E
Sinnaeve
Davy
D
Martins
José C
JC
Topgaard
Daniel
D
Meeren
Paul Van der
PV
D000327:Adsorption; D019905:Benzyl Alcohol / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D008954:Models, Biological; D044367:Phase Transition; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013010:Sonication
D008051:Lipid Bilayers; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; D019905:Benzyl Alcohol