title: Modulatory role of copper on β-naphthoflavone-induced DNA damage in European eel (Anguilla anguilla L.).
abstract: Gill and kidney DNA integrity (alkaline unwinding assay) was assessed in Anguilla anguilla exposed for 24-h to copper (Cu: 1 or 2.5 μM), with or without 24-h pre-exposure to a polycyclic aromatic hydrocarbon (PAH)-like compound--β-naphthoflavone (BNF: 2.7 μM). Gill showed DNA integrity loss in all the exposure conditions, reflecting a dual mode of BNF-Cu interaction depending on the metal concentration. Thus, antagonistic or additive effects were observed for BNF+Cu 1 μM or BNF+Cu 2.5 μM, respectively. Kidney showed decreased DNA integrity for single exposures (BNF, Cu 1 μM), whereas sequential exposures displayed higher DNA integrity than BNF alone, revealing a Cu antagonistic effect at both the concentrations. The results also demonstrated that (i) both organs are receptive for Cu inhibitory role against BNF genotoxicity; (ii) kidney is more resistant to Cu individual exposures; and (iii) under multi-pollution conditions genotoxicity cannot be predicted on the basis of individual chemicals responses.
authors.affiliation: CESAM & Animal Physiology/Ecotoxicology Sector, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal. ahmadr@ua.pt
authors.forename: I
authors.initials: I
authors.lastname: Ahmad
authors.forename: V L
authors.initials: VL
authors.lastname: Maria
authors.forename: M
authors.initials: M
authors.lastname: Oliveira
authors.forename: M
authors.initials: M
authors.lastname: Pacheco
authors.forename: M A
authors.initials: MA
authors.lastname: Santos
mesh_terms: D000811:Anguilla / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D003300:Copper / Q000633:toxicity*; D004249:DNA Damage; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000633:toxicity; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D019324:beta-Naphthoflavone / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D004791:Enzyme Inhibitors; D009153:Mutagens; D014874:Water Pollutants, Chemical; D019324:beta-Naphthoflavone; D003300:Copper