title: A high oocyte yield for intracytoplasmic sperm injection treatment is associated with an increased chromosome error rate.
abstract: OBJECTIVE
  To compare the chromosome error rate among oocytes from stimulated ovaries after retrieval of 1-5 oocytes, 6-10 oocytes, and >10 oocytes.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  A university-based human genetic institute in collaboration with a private fertility center.
  
  
  METHODS
  Nine hundred thirty-three women undergoing intracytoplasmic sperm injection (ICSI) with a poor prognosis.
  
  
  METHODS
  Oocyte collection with ovarian stimulation. Polar body testing of ICSI oocytes for common chromosome errors.
  
  
  METHODS
  Chromosome error rate in oocytes, as determined by five-color fluorescence in situ hybridization.
  
  
  RESULTS
  In women less than 35 years and women between 35 and 40 years undergoing the first ICSI cycle, oocytes from the high-yield group had an increased likelihood for detectable chromosome errors (50.9% and 54.6%, respectively), compared to the intermediate-yield group (34.9% and 43.8%) and the low-yield group (23.3% and 41.2%). The overall high rate (>or=50%) of chromosomally abnormal oocytes in women more than 40 years appeared to be mainly due to the maternal age effect and increased only slightly with oocyte yield.
  
  
  CONCLUSIONS
  Oocyte yield may be considered as an indicator of ovarian response to hormone stimulation. In women up to 40 years a high yield of oocytes after superovulation is associated with an increased chromosome error rate.
authors.affiliation: Institute for Human Genetics, Epidemiology and Informatics, Johannes Gutenberg University Mainz, Mainz, Germany. haaf@humgen.klinik.uni-mainz.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Haaf
authors.forename: Antje
authors.initials: A
authors.lastname: Hahn
authors.forename: Anne
authors.initials: A
authors.lastname: Lambrecht
authors.forename: Bärbel
authors.initials: B
authors.lastname: Grossmann
authors.forename: Eva
authors.initials: E
authors.lastname: Schwaab
authors.forename: Omar
authors.initials: O
authors.lastname: Khanaga
authors.forename: Thomas
authors.initials: T
authors.lastname: Hahn
authors.forename: Achim
authors.initials: A
authors.lastname: Tresch
authors.forename: Martin
authors.initials: M
authors.lastname: Schorsch
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000139:chemically induced*; D015331:Cohort Studies; D005260:Female; D005300:Fertility Agents, Female / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008423:Maternal Age; D054315:Oocyte Retrieval; D010062:Ovulation Induction / Q000009:adverse effects*; D011247:Pregnancy; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome
chemical_list: D005300:Fertility Agents, Female