Dissolution of biomacromolecules in organic solvents by nano-complexing with poly(ethylene glycol).
Various biomacromolecules (BMs) such as proteins, DNA, and carbohydrates are extremely difficult to be dissolved in a single organic solvent phase for sustained release or targeted delivery formulation. In this study, three different BMs could be solubilized in selected organic solvents by forming poly(ethylene glycol) (PEG)-assisted nano-complexes while maintaining their structural integrity. Dynamic light scattering (DLS) and atomic force microscopy (AFM) analysis revealed that proteins, DNA, and carbohydrate polymers could be nano-complexed with PEG in various organic solvents. The diameter of nano-complexes decreased roughly from approximately 600 nm to approximately 100 nm with increasing weight ratio of PEG/BM. The present solubilization technique could be potentially applied for sustained release formulations of various therapeutic biological drugs.
Mok
Hyejung
H
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea.
Kim
Ho Jeong
HJ
Park
Tae Gwan
TG
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D008027:Light; D008297:Male; D018625:Microscopy, Atomic Force; D009930:Organic Chemicals / Q000737:chemistry; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D012474:Salmon; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013094:Spermatozoa / Q000737:chemistry
D003692:Delayed-Action Preparations; D004337:Drug Carriers; D009930:Organic Chemicals; D012997:Solvents; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D004247:DNA