Catalase and superoxide dismutase activities in a Stenotrophomonas maltophilia WZ2 resistant to herbicide pollution.
Quinclorac bensulfuron-methyl is a mixed herbicide widely used on paddy rice field to effectively control barnyard grass and most broad-leaved grasses and sedges. We analyzed superoxide dismutase (SOD) and catalase activities in the quinclorac-highly degrading strain Stenotrophomonas maltophilia WZ2 and Gram-negative standard strain Escherichia coli K12 in an attempt to understand antioxidant enzymes in bacteria are produced in response to quinclorac or bensulfuron-methyl, which increases the virulence of the bacteria. MnSOD and two additional catalase isozymes were induced by quinclorac or bensulfuron-methyl in S. maltophilia WZ2, but not in E. coli K12. Quinclorac turned out to be a more sensitive inducer of SOD, whereas bensulfuron-methyl is a more sensitive one of catalase. A mixture of both has effects similar to quinclorac. Results indicate that catalase has a much weakly role in the defense against quinclorac or bensulfuron-methyl induced oxidative stress, whereas SOD could be critical.
Lü
Zhenmei
Z
Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310058, China.
Sang
Liya
L
Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310058, China.
Li
Zimu
Z
Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310058, China.
Min
Hang
H
Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310058, China. Electronic address: minhang@zju.edu.cn.
D001426:Bacterial Proteins / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000187:drug effects / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D048168:Escherichia coli K12 / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000187:drug effects / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D011804:Quinolines / Q000633:toxicity*; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013453:Sulfonylurea Compounds / Q000633:toxicity; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006540:Herbicides; D011804:Quinolines; D013453:Sulfonylurea Compounds; C489048:quinclorac; C071475:methyl bensulfuron; D002374:Catalase; D013482:Superoxide Dismutase