Microbial biodegradation and toxicity of vinclozolin and its toxic metabolite 3,5-dichloroaniline.
Vinclozolin, an endocrine disrupting chemical, is a chlorinated fungicide widely used to control fungal diseases. However, its metabolite 3,5-dichloroaniline is more toxic and persistent than the parent vinclozolin. For the biodegradation of vinclozolin, vinclozolin- and/or 3,5-dichloroaniline-degrading bacteria were isolated from pesticide-polluted agriculture soil. Among the isolated bacteria, a Rhodococcus sp. was identified from a 16S rDNA sequence analysis and named Rhodococcus sp. T1-1. The degradation ratios for vinclozolin or 3,5- dichloroaniline in a minimal medium containing vinclozolin (200 microg/ml) or 3,5-dichloroaniline (120 microg/ml) were 90% and 84.1%, respectively. Moreover, Rhodococcus sp. T1-1 also showed an effective capability to biodegrade dichloroaniline isomers on enrichment cultures in which they were contained. Therefore, these results suggest that Rhodococcus sp. T1-1 can bioremediate vinclozolin as well as 3,5-dichloroaniline.
Lee
Jung-Bok
JB
School of Bioresource Sciences, Andong National University, Andong 760-749, Korea.
Sohn
Ho-Yong
HY
Shin
Kee-Sun
KS
Kim
Jong-Sik
JS
Jo
Min-Sub
MS
Jeon
Chun-Pyo
CP
Jang
Jong-Ok
JO
Kim
Jang-Eok
JE
Kwon
Gi-Seok
GS
D000814:Aniline Compounds / Q000378:metabolism* / Q000633:toxicity; D001673:Biodegradation, Environmental; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010080:Oxazoles / Q000378:metabolism* / Q000633:toxicity; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012988:Soil Microbiology; D015003:Yeasts / Q000187:drug effects
D000814:Aniline Compounds; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010080:Oxazoles; D012336:RNA, Ribosomal, 16S; C025643:vinclozolin; C037267:3,5-dichloroaniline