title: [Establishment of an animal model of oral mucositis induced by conditioning regimen of haematopoietic stem cell transplantation].
abstract: OBJECTIVE
  To establish a rat model of oral mucositis (OM) induced by busulfan and cyclophosphamide (BUCY) conditioning regimen of hematopoietic stem cell transplantation (HSCT).
  
  
  METHODS
  In the model group, busulfan (6.0 mg.kg(-1).d(-1) x 4 d) and cyclophosphamide (120 mg.kg(-1).d(-1) x 2 d) were administered by intra-stomach perfusion and intraperitoneal injection, respectively. The left cheek mucosa were irritated by superficial scratching on day 6. The oral mucosal score (OMS) was assessed daily. Animals were sacrificed on day 7, 10, 13, 16 and 18. The samples of blood, bone marrow, and the oral mucosa were harvest to evaluate the clinical and histological changes.
  
  
  RESULTS
  The incidence of oral mucositis in model group was as high as 80.00% with a survival rate of 73.33%. The initial lesion on the oral mucosa was noted on day 7 with red spot and edema, and then progressive mucositis was characterized by large areas of ulcer formation. The duration of oral mucositis was 8 to 10 days. A continuous weight loss, white blood cell count decrease and bone marrow suppression occurred in the process of oral mucositis.
  
  
  CONCLUSIONS
  An animal model of conditioning regimen-induced oral mucositis was successfully established.
authors.affiliation: Department of Oral Medicine, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China. chengbin@mail.sysu.edu.cn
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Chen
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Xiu-Zhen
authors.initials: XZ
authors.lastname: Tong
mesh_terms: D000818:Animals; D002066:Busulfan / Q000633:toxicity; D003520:Cyclophosphamide / Q000633:toxicity; D004195:Disease Models, Animal; D005240:Feasibility Studies; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D009061:Mouth Mucosa / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013280:Stomatitis / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology; D019172:Transplantation Conditioning / Q000009:adverse effects*
chemical_list: D003520:Cyclophosphamide; D002066:Busulfan