Formation of capillary tube-like structures on micropatterned biomaterials.
The survival of three-dimensional tissue requires a vascular network to provide transport of oxygen and metabolic byproduct. Here, we report a new approach to create capillary blood vessels in vitro on biomaterials suitable for use as scaffolds in engineering tissues. Endothelial cells were cultured on chemical and topographical patterns of micro-sized grooves on gelatin. Selective attachment and spreading of cells within the grooves was ensured by microcontact printing the plateau regions with cell resistant PEG/PLA (polyethyleneglycol-L-polylacticacid). Human microvascular endothelial cells plated on these patterned biomaterials attached and spread exclusively within the grooves. These topographical features promote endothelial cells to form capillary tube-like structures. The results demonstrated that capillary structures formed on biomaterials are useful for engineering vascularized tissues.
Gao
Dahai
D
Department of Chemical Engineering, University of Cincinnati, Cincinnati, OH, USA.
Kumar
Girish
G
Co
Carlos
C
Ho
Chia-Chi
CC
D001672:Biocompatible Materials; D002196:Capillaries / Q000166:cytology* / Q000254:growth & development*; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology; D005452:Fluoresceins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D011091:Polyesters; D011092:Polyethylene Glycols / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000378:metabolism; D013997:Time Factors
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011091:Polyesters; D011108:Polymers; D011506:Proteins; C069306:5-chloromethylfluorescein; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; C033616:poly(lactide); D005780:Gelatin; D005976:Glutaral