Antioxidant and hepatoprotective activities of Cirsium setidens Nakai against CCl4-induced liver damage.
The antioxidant activity and hepatoprotective potential of Cirsium setidens Nakai, a widely used medicinal plant, were investigated. The n-butanol (n-BuOH) fraction of leaves and roots of C. setidens had a higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the other soluble fractions. The n-BuOH fraction of roots of C. setidens had a significant hepatoprotective activity at a dose of 500 mg/kg compared to that of a standard agent. The biochemical results were confirmed by histological observations indicating that C. setidens extract decreased ballooning degeneration in response to CCl(4) treatment. The n-BuOH fraction reduced CCl(4)-induced liver injury in rats, and transcript levels of genes encoding antioxidant enzymes such as glutathione peroxidase 1 (GPO1), glutathione peroxidase 3 (GPO3) and superoxide dismutase (SOD1) were elevated in the livers of rats treated with this fraction (500 mg/kg). Based on these results, we suggest that the C. setidens extract has hepatoprotective effect related to its antioxidant activity.
Lee
Sung Hyun
SH
School of Biotechnology, Kangwon National University, Chuncheon, Kangwon-do, 200-701, South Korea.
Heo
Seong-Il
SI
Li
Lan
L
Lee
Min Jie
MJ
Wang
Myeong-Hyeon
MH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D031180:Cirsium; D004247:DNA / Q000235:genetics; D000431:Ethanol; D016166:Free Radical Scavengers; D006834:Hydrazines; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008107:Liver Diseases / Q000517:prevention & control*; D000069463:Olive Oil; D010851:Picrates; D010936:Plant Extracts / Q000627:therapeutic use*; D010938:Plant Oils; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D000069463:Olive Oil; D010851:Picrates; D010936:Plant Extracts; D010938:Plant Oils; D000431:Ethanol; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl