title: Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development.
abstract: The COP9 signalosome (CSN) is a multisubunit regulator highly conserved in evolution. We show here that CSN subunit 8 (CSN8) is essential for Drosophila development. CSN8 is maternally contributed and present throughout development. Null mutants generated in this study are larval lethal, showing phenotypes associated with mutations in either CSN4 (molting defects) or CSN5 (melanotic tumors). Analysis of mitotic and germ-line csn8(null) clones revealed the requirement of CSN8 for multiple developmental processes. The germ-line clones arrested at mid-oogenesis, while the mitotic clones led to deformed adult eyes or wings. CSN8 is present exclusively as part of the CSN holo-complex, and lack of CSN8 in the mutants leads to CSN instability. Consistent with this, Cullin deneddylation is impaired in the csn8(null) mutants.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Pazit
authors.initials: P
authors.lastname: Oren-Giladi
authors.forename: Ofra
authors.initials: O
authors.lastname: Krieger
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Edgar
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Chamovitz
authors.forename: Daniel
authors.initials: D
authors.lastname: Segal
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D030801:Animals, Genetically Modified; D000075686:COP9 Signalosome Complex; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D018390:Gene Targeting; D005804:Genes, Lethal; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008018:Life Cycle Stages / Q000235:genetics; D008540:Meiosis; D008545:Melanoma / Q000235:genetics*; D046912:Multiprotein Complexes / Q000235:genetics*; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009866:Oogenesis / Q000235:genetics; D010447:Peptide Hydrolases / Q000235:genetics*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C520406:CSN4 protein, Drosophila; C531359:CSN8 protein, Drosophila; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D012333:RNA, Messenger; D010447:Peptide Hydrolases; C501491:CSN5 protein, Drosophila; D000075686:COP9 Signalosome Complex