title: Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus (V).
abstract: The oocytes of the abyssal Teleost, Hoplostethus mediterraneus were studied. Four stages of growth were observed and the oocytes of all the stages were surrounded by follicular cells and had several nucleoli in the nucleus. In the oocytes of the II degrees stage, vacuoles without contents, in oocytes of the III degrees stage several vacuoles with a basophilic contents and small yolk globules were identified. General and basic proteins, ribonucleoproteins, acid proteoglycans with -COOH groups were recognized in the cytoplasm, in the nucleoli of oocytes in the II degrees stage and in the vacuolar contents of oocytes in the III degrees stage. In the follicular cells, in the pellucid zone, in the yolk globules, from their beginning, glycoproteins were present. Positivity, for all lectins used, was revealed in the follicular cells and in the four stages of oocytes growth. alpha-D-glucose and alpha-D-mannose binding sites were in the pellucid zone and in the initial yolk globules. In the lather galactose and beta-N-acetyl glucosamine were present too. nNOS and VIP immunopositivity revealed at the periphery of the cytoplasm and at network of nerve fibres between oocytes, suggests NO is involved in a mechanism of regulation of the gametogenesis and of the spawning.
authors.affiliation: Department of Animal Biology and Marine Ecology, Faculty of Science University, Messina, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Calabro
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Albanese
authors.forename: Clara
authors.initials: C
authors.lastname: Bertuccio
authors.forename: Aurelio
authors.initials: A
authors.lastname: Licata
authors.forename: Nunzia
authors.initials: N
authors.lastname: Gentile
mesh_terms: D000818:Animals; D005260:Female; D005399:Fishes / Q000254:growth & development*; D006001:Glycoconjugates / Q000378:metabolism; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000254:growth & development*; D010053:Ovary / Q000166:cytology; D014818:Vitellogenesis
chemical_list: D006001:Glycoconjugates; D037102:Lectins