Simultaneous detection of airborne aflatoxin, ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography.
An AOZ method, based on high-performance liquid chromatography (HPLC), was optimized on HPLC condition such as mobile phase and wavelength to simultaneously quantify six kinds of mycotoxins [four aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA)]. Conditions for immunoaffinity clean-up, HPLC and photo-derivatization were optimized in this study and successfully applied in assessment of airborne mycotoxins from a poultry house in Dalian, China. Fifty-two air samples were collected with AGI-30 air samplers using pure water as collection media. Twenty air samples (20/52, 38.46%) were positive for four toxins. Among the positive samples, airborne mycotoxin concentrations (mean+/-S.D.) for AFG(2), AFB(1), and ZEA were 0.189+/-0.024 (n=9), 0.080+/-0.003 (n=11) and 2.363+/-0.030 (n=5)ng/m(3) air, while the concentration for OTA was 8.530 (n=1)ng/m(3). No positive sample was found for either AFG(1) or AFB(2). A chicken may inhale 0.019-0.057 ng AFG(2), 0.013-0.019 ng AFB(1), 0.436-0.513 ng ZEA, and 1.706 ng OTA, respectively, in a day. A poultry worker may inhale 0.504-1.512 ng AFB(1), 0.752-2.28 ng AFG(2), 68.240 ng OTA, and 17.432-20.512 ng ZEA in a working day. This is the first report on airborne mycotoxins in poultry house. These data may have importance in animal and public health implications.
Wang
Yaling
Y
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, Shandong, Taian 271018, PR China.
Chai
Tongjie
T
Lu
Guozhong
G
Quan
Chunsan
C
Duan
Huiyong
H
Yao
Meiling
M
Zucker
Bert-Andree
BA
Schlenker
Gerd
G
D000388:Air / Q000032:analysis*; D000393:Air Pollutants / Q000032:analysis; D016902:Air Pollution, Indoor / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006799:Housing, Animal / Q000592:standards*; D006801:Humans; D007163:Immunosorbent Techniques; D009183:Mycotoxins / Q000032:analysis*; D011200:Poultry
D000393:Air Pollutants; D009183:Mycotoxins