title: DeepSAGE: higher sensitivity and multiplexing of samples using a simpler experimental protocol.
abstract: Combining serial analysis of gene expression (SAGE) with pyrophosphatase-based ultra-high-throughput DNA sequencing provides increased sensitivity and cost-effective gene expression profiling. The combined techniques obviate the formation and cloning of concatemers and the tedious picking and preparation of sequence templates from bacterial clones that are necessary with SAGE alone. Furthermore, multiplexing of samples or replicates of analysis is included in the experimental design.
authors.affiliation: Department of Life Sciences, University of Aalborg, Aalborg, Denmark.
authors.forename: Kåre Lehmann
authors.initials: KL
authors.lastname: Nielsen
mesh_terms: D001483:Base Sequence; D003362:Cost-Benefit Analysis; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D020869:Gene Expression Profiling / Q000191:economics / Q000379:methods* / Q000706:statistics & numerical data; D021141:Nucleic Acid Amplification Techniques; D011755:Pyrophosphatases; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D018076:DNA, Complementary; C049760:endodeoxyribonuclease MmeI; C048422:endodeoxyribonuclease NlaIII; D015252:Deoxyribonucleases, Type II Site-Specific; D011755:Pyrophosphatases