Identification of CKAP4/p63 as a major substrate of the palmitoyl acyltransferase DHHC2, a putative tumor suppressor, using a novel proteomics method.
Protein palmitoylation is the post-translational addition of the 16-carbon fatty acid palmitate to specific cysteine residues by a labile thioester linkage. Palmitoylation is mediated by a family of at least 23 palmitoyl acyltransferases (PATs) characterized by an Asp-His-His-Cys (DHHC) motif. Many palmitoylated proteins have been identified, but PAT-substrate relationships are mostly unknown. Here we present a method called palmitoyl-cysteine isolation capture and analysis (or PICA) to identify PAT-substrate relationships in a living vertebrate system and demonstrate its effectiveness by identifying CKAP4/p63 as a substrate of DHHC2, a putative tumor suppressor.
Zhang
Jun
J
The Whitney Laboratory, Department of Neuroscience, University of Florida, St. Augustine, Florida 32080, USA.
Planey
Sonia L
SL
Ceballos
Carolina
C
Stevens
Stanley M
SM
Keay
Susan K
SK
Zacharias
David A
DA
D000217:Acyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D004526:Efficiency; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology*; D008954:Models, Biological; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
C081876:CKAP4 protein, human; D008565:Membrane Proteins; D025521:Tumor Suppressor Proteins; D010171:Palmitoyl Coenzyme A; D000217:Acyltransferases; C532036:ZDHHC2 protein, human