Upregulation of annexin A2 in H(2)O(2)-induced premature senescence as evidenced by 2D-DIGE proteome analysis.
Human diploid fibroblasts undergo premature senescence after treatment with sublethal concentration of H(2)O(2). We report the first proteomic study of microsomal proteins in the context of H(2)O(2)-induced premature senescence by using 2D-DIGE approach. Twelve different proteins with altered abundance at day 3 after treatment with H(2)O(2) were identified. Among them, we demonstrated a re-localization of annexin A2 in plasma membrane.
Chrétien
Aline
A
Unit of Research on Cellular Biology, University of Namur (FUNDP), Belgium.
Delaive
Edouard
E
Dieu
Marc
M
Demazy
Catherine
C
Ninane
Noëlle
N
Raes
Martine
M
Toussaint
Olivier
O
D000375:Aging / Q000187:drug effects / Q000378:metabolism*; D019588:Aging, Premature / Q000139:chemically induced / Q000378:metabolism; D017306:Annexin A2 / Q000187:drug effects / Q000378:metabolism*; D004171:Diploidy; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D011506:Proteins / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation
C472822:ANXA2 protein, human; D017306:Annexin A2; D016877:Oxidants; D011506:Proteins; D012333:RNA, Messenger; D006861:Hydrogen Peroxide