title: Store-operated Ca2+ influx causes Ca2+ release from the intracellular Ca2+ channels that is required for T cell activation.
abstract: The precise control of many T cell functions relies on cytosolic Ca(2+) dynamics that is shaped by the Ca(2+) release from the intracellular store and extracellular Ca(2+) influx. The Ca(2+) influx activated following T cell receptor (TCR)-mediated store depletion is considered to be a major mechanism for sustained elevation in cytosolic Ca(2+) concentration ([Ca(2+)](i)) necessary for T cell activation, whereas the role of intracellular Ca(2+) release channels is believed to be minor. We found, however, that in Jurkat T cells [Ca(2+)](i) elevation observed upon activation of the store-operated Ca(2+) entry (SOCE) by passive store depletion with cyclopiazonic acid, a reversible blocker of sarco-endoplasmic reticulum Ca(2+)-ATPase, inversely correlated with store refilling. This indicated that intracellular Ca(2+) release channels were activated in parallel with SOCE and contributed to global [Ca(2+)](i) elevation. Pretreating cells with (-)-xestospongin C (10 microM) or ryanodine (400 microM), the antagonists of inositol 1,4,5-trisphosphate receptor (IP3R) or ryanodine receptor (RyR), respectively, facilitated store refilling and significantly reduced [Ca(2+)](i) elevation evoked by the passive store depletion or TCR ligation. Although the Ca(2+) release from the IP3R can be activated by TCR stimulation, the Ca(2+) release from the RyR was not inducible via TCR engagement and was exclusively activated by the SOCE. We also established that inhibition of IP3R or RyR down-regulated T cell proliferation and T-cell growth factor interleukin 2 production. These studies revealed a new aspect of [Ca(2+)](i) signaling in T cells, that is SOCE-dependent Ca(2+) release via IP3R and/or RyR, and identified the IP3R and RyR as potential targets for manipulation of Ca(2+)-dependent functions of T lymphocytes.
authors.affiliation: Department of Physiology and Membrane Biology, University of California, Davis, Davis, California 95616, USA.
authors.forename: Sepehr
authors.initials: S
authors.lastname: Dadsetan
authors.forename: Liudmila
authors.initials: L
authors.lastname: Zakharova
authors.forename: Tadeusz F
authors.initials: TF
authors.lastname: Molinski
authors.forename: Alla F
authors.initials: AF
authors.lastname: Fomina
mesh_terms: D015220:Calcium Channels / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003620:Dantrolene / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000037:antagonists & inhibitors; D007376:Interleukin-2 / Q000096:biosynthesis; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D047028:Macrocyclic Compounds / Q000494:pharmacology; D018448:Models, Immunological; D010080:Oxazoles / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000819:agonists; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects
chemical_list: D015220:Calcium Channels; D007211:Indoles; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007376:Interleukin-2; D047028:Macrocyclic Compounds; D010080:Oxazoles; D011948:Receptors, Antigen, T-Cell; D019837:Ryanodine Receptor Calcium Release Channel; C511704:xestospongin C; D012433:Ryanodine; D003620:Dantrolene; C000543:cyclopiazonic acid