Preparation of monolithic silica columns for high-performance liquid chromatography.
Preparation methods of monolithic silica columns for HPLC including the surface modification were reviewed. Chemical modification methods recently reported to obtain stationary phases for reversed-phase (RP), chiral, ion-exchange, and hydrophilic interaction chromatography (HILIC) separations were discussed. Recent results related to preparation methods of monolithic silica were also covered. The characteristics and properties of silica monoliths and some applications of monolithic silica columns for different analytical and bioanalytical fields will be commented.
Núñez
Oscar
O
Department of Analytical Chemistry, Faculty of Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain. oscarnubu@yahoo.es
Nakanishi
Kazuki
K
Tanaka
Nobuo
N
D000596:Amino Acids / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D003619:Dansyl Compounds / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism
D000596:Amino Acids; D003619:Dansyl Compounds; D012822:Silicon Dioxide