Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria.
The objective of the present study was to identify mitochondrial components associated with the damage caused by iron to the rat heart. Decreased cell viability was assessed by increased presence of lactate dehydrogenase (LDH) in serum. To assess the functional integrity of mitochondria, Reactive Oxygen Species (ROS), the Respiratory Control Ratio (RCR), ATP and chelatable iron content were measured in the heart. Chelatable iron increased 15-fold in the mitochondria and ROS increased by 59%. Deterioration of mitochondrial function in the presence of iron was demonstrated by low RCR (46% decrease) and low ATP content (96% decrease). Using two dimensional gel electrophoresis (2DE), we identified alterations in 21 mitochondrial proteins triggered by iron overload. Significantly, expression of the alpha, beta, and d subunits of F(1)F(o) ATP synthase increased along with the loss of ATP. This suggests that the F(1)F(o) ATP synthase participates in iron metabolism.
Kim
Misun
M
Division of Life Science, College of Natural Sciences, Sookmyung Women's University, Seoul, Korea.
Kim
Jinsun
J
Cheon
Choong-Ill
CI
Cho
Dae Ho
DH
Park
Jong Hoon
JH
Kim
Keun Il
KI
Lee
Kyo-Young
KY
Song
Eunsook
E
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D002712:Chlorides; D015180:Electrophoresis, Gel, Two-Dimensional; D005290:Ferric Compounds / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
D002712:Chlorides; D005290:Ferric Compounds; D005343:Fibrinolytic Agents; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; C024555:ferric chloride