Reduction of acrylamide and its kinetics by addition of antioxidant of bamboo leaves (AOB) and extract of green tea (EGT) in asparagine-glucose microwave heating system.
This study investigated the effect of antioxidant of bamboo leaves (AOB) and extract of green tea (EGT) on the formation and kinetics of acrylamide in an equimolar asparagine-glucose model system. The substrates spiked with AOB and EGT were microwave-heated at 180 degrees C and the acrylamide content in final reaction products was quantified by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that both AOB and EGT could effectively reduce the formation of acrylamide in an asparagine-glucose microwave heating model system and achieved a maximum reduction rate when the addition levels of AOB and EGT were both 10(-6) mg/mL reaction solution. To describe the kinetic behavior of acrylamide, a simplified kinetic model was optimized and relative kinetic rate constants were evaluated under isothermal conditions. The results indicated that the reduction effect of AOB and EGT on the acrylamide formation may partly be ascribed to the decrease of the formation rate constant (k(F)) in both AOB and EGT-spiked systems (43.4% and 32.3% of decrease, respectively, P < 0.05). The kinetic parameter k(E), which represents the elimination rate of acrylamide in both AOB and EGT-spiked systems, was not significantly different (6.9% of increase and 10.9% of decrease, respectively, P > 0.05). The results of the kinetic study indicated that addition of AOB and EGT could significantly reduce the formation rate constant (k(F)) of acrylamide, but could not significantly affect the elimination rate constant (k(E)) of acrylamide.
Zhang
Yu
Y
Dept. of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang Univ., Hangzhou 310029, Zhejiang, China. y_zhang@zju.edu.cn
Ying
Tiejin
T
Zhang
Ying
Y
D020106:Acrylamide / Q000032:analysis / Q000737:chemistry*; D000975:Antioxidants / Q000494:pharmacology*; D001216:Asparagine / Q000737:chemistry; D002273:Carcinogens / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D005511:Food Handling / Q000379:methods*; D005947:Glucose / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008872:Microwaves; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D031787:Sasa / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D013662:Tea / Q000737:chemistry*
D000975:Antioxidants; D002273:Carcinogens; D010936:Plant Extracts; D013662:Tea; D020106:Acrylamide; D001216:Asparagine; D005947:Glucose