Large scale hippocampal cellular distress may explain the behavioral consequences of repetitive traumatic experiences--a proteomic approach.
Early life traumatic experiences are associated with psychopathology in adulthood. This may be due in part to the effects of trauma on hippocampal development and protein expression. The purpose of the study was to investigate the effects of early life trauma and adult re-stress on ventral hippocampal protein expression. Adolescent rats (n = 19) were subjected to a triple stressor on post-natal day 28 followed 7 days later by the first re-stress session and 25 days later (post-natal day 60 = adulthood) by the second re-stress session. Ventral hippocampi were collected on post-natal day 68 for protein expression determinations using protein arrays and 2D-gel electrophoresis with liquid chromatography tandem mass spectrometry. Compared to controls, traumatized animals showed an increase in Ca(2+) homeostatic proteins, dysregulated signaling pathways and energy metabolism enzymes, cytoskeletal protein changes, a decrease in neuroplasticity regulators, energy metabolism enzymes and an increase in apoptotic initiator proteins. These results indicate the extensive impact of trauma on adult brain development and behavior.
Uys
Joachim D K
JD
Division of Medical Physiology, Department of Biomedical Sciences, University of Stellenbosch, Tygerberg Campus, Francie van Zijl Avenue, Parow Valley, 7505, Cape Town, Western Cape, South Africa.
Hattingh
Suzél M
SM
Stein
Dan J
DJ
Daniels
Willie M U
WM
D000328:Adult; D000818:Animals; D001921:Brain / Q000502:physiology; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D001523:Mental Disorders / Q000209:etiology / Q000503:physiopathology; D008969:Molecular Sequence Data; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000150:complications / Q000523:psychology
D020543:Proteome