A liquid chromatography tandem mass spectrometry method for the quantification of indocyanine green in dog plasma and bile.
A sensitive and specific liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of indocyanine green (ICG) in dog plasma and bile. An ICG analog (IR-820) was used as an internal standard. A protein precipitation method was used with acetonitrile for plasma sample preparation, whereas bile samples were diluted with water (120-fold) prior to analysis. Using MS/MS in the multiple reaction monitoring mode, ICG and IR-820 were detected in both matrices without interference. The lower limit of quantitation for ICG in dog plasma was 3 ng/mL, with an intra- and inter-day accuracy (%Bias) and precision (%CV) of less than 15%, so it was possible to study the pharmacokinetics of ICG in bile duct-cannulated dogs and assess their liver function after surgery. The method described herein is sensitive, selective and faster than other existing methods (e.g., spectrophotometry, HPLC/UV-vis detection, or HPLC/fluorescence detection).
Chen
Cliff Y
CY
Metabolism and Pharmacokinetics, Pharmaceutical Candidate Optimization, Bristol-Myers Squibb Co, Princeton, NJ 08543, USA.
Fancher
R Marc
RM
Ruan
Qian
Q
Marathe
Punit
P
Rodrigues
A David
AD
Yang
Zheng
Z
D000818:Animals; D000838:Anions / Q000032:analysis* / Q000097:blood; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D004285:Dogs; D007208:Indocyanine Green / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000838:Anions; D004396:Coloring Agents; D007208:Indocyanine Green