Expression of a splicing isoform of MAIR-V (CD300LF), an inhibitory immunoglobulin-like receptor on myeloid cells.
The myeloid-associated immunoglobulin-like receptor (MAIR) family consists of nine activating or inhibitory cell surface receptors preferentially expressed on myeloid cells, which are encoded by the genes in a small cluster on mouse chromosome 11. MAIR-Va, one of the inhibitory receptors in the family, has a splicing isoform, MAIR-Vb. However, the expression profile of MAIR-Va and MAIR-Vb has remained unclear. Here, we generated three monoclonal antibodies (MAb)-TX70, TX71, and TX73-against MAIR-V. TX70 and TX73 MAbs recognized both MAIR-Va and MAIR-Vb. In contrast, TX71 recognized MAIR-Va, but not MAIR-Vb. Western blot analyses by using these MAbs revealed that MAIR-Va and MAIR-Vb were almost equally expressed in the spleen cells and peritoneal exudate cells. Thus, these MAbs are useful for analyses of molecular and functional characteristics of MAIR-Va and MAIR-Vb.
Can
Ismail
I
Department of Immunology, Institute of Basic Medical Sciences and Center for TARA, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
Tahara-Hanaoka
Satoko
S
Shibuya
Akira
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D005122:Exudates and Transudates / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism*; D010529:Peritoneal Cavity; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D012326:RNA Splicing; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000378:metabolism
D000911:Antibodies, Monoclonal; C482560:CLM-1 protein, mouse; D020033:Protein Isoforms; D011971:Receptors, Immunologic