[Proteasome activity changes in the aorta, heart tissues, and blood leucocytes in modelling of cholesterol atherosclerosis].
In experiments on modelling of cholesterol atherosclerosis in rabbits (1% cholesterol-rich diet during 2 month) it was determined changes of trypsin-like (TL), chymotrypsin-like (CHTL) and peptydilglutamilpeptidase (PGPG) proteasomal activity in tissues of aorta, heart and isolated blood leukocytes. It was shown that cholesterol-rich diet caused significant increase of TL (3.2 fold, P=0.003), PGPG (1.8 fold, P=0.003) proteasomal activity in aorta tissues, and PGPG activity (1.8-times, P=0.003) in myocardium. In isolated blood monocytes, the CHTL and PGPG activities were significantly increased (1.9 fold, P=0.05 and 11.6 fold, P=0.0001, respectively) and in PMN leucocytes the PGPG activity of proteasome was also significantly increased (1.8 fold, P=0.031). Proteasomal activity in lymphocytes during cholesterol atherosclerosis modelling had no significant changes. The data obtained indicate that alimentary hypercholesterolemia induces considerable changes of proteasomal activity in cardio-vascular system and blood cells that take part in atherogenesis.
Pashevin
D O
DO
Dosenko
V Ie
VIe
Byts'
Iu V
IuV
Moĭbenko
O O
OO
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D050197:Atherosclerosis / Q000097:blood / Q000201:enzymology / Q000209:etiology; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D004036:Diet, Atherogenic; D004195:Disease Models, Animal; D005260:Female; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D011817:Rabbits
D002791:Cholesterol, Dietary; D046988:Proteasome Endopeptidase Complex