[Formation of the exopolysaccharide ethapolan by Acinetobacter sp. IMV B-7005 on a fumarate-glucose mixture].
Our studies enabled us to intensify the synthesis of the microbial exopolysaccharide (EPS) ethapolan produced by Acinetobacter sp. IMV B-7005 grown on a mixture of fumarate (an energy-excessive substrate) and glucose (an energy-deficient substrate). Supplementing glucose-containing medium with sodium (potassium) fumarate at a molar ratio of 4:1 resulted in a 1.3-2.2-fold increase of the EPS amount synthesized and in a 1.3-2-fold increase of the EPS yield relative to the biomass compared to monosubstrate cultivation. The conversion of the carbon of both substrates to EPS was the highest if the carbon/nitrogen ratio in the cultivation medium was 70.5 and inoculum grown on glucose monosubstrate was used.
Pirog
T P
TP
Vysiatetskaia
N V
NV
Korzh
Iu V
IuV
D000150:Acinetobacter / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005650:Fumarates; D005947:Glucose; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D013379:Substrate Specificity
D003470:Culture Media; D005650:Fumarates; D011135:Polysaccharides, Bacterial; C032005:fumaric acid; D005947:Glucose