Molecular and phenotypic profiling from the base to the crown in maritime pine wood-forming tissue.
Environmental, developmental and genetic factors affect variation in wood properties at the chemical, anatomical and physical levels. Here, the phenotypic variation observed along the tree stem was explored and the hypothesis tested that this variation could be the result of the differential expression of genes/proteins during wood formation. Differentiating xylem samples of maritime pine (Pinus pinaster) were collected from the top (crown wood, CW) to the bottom (base wood, BW) of adult trees. These samples were characterized by Fourier transform infrared spectroscopy (FTIR) and analytical pyrolysis. Two main groups of samples, corresponding to CW and BW, could be distinguished from cell wall chemical composition. A genomic approach, combining large-scale production of expressed sequence tags (ESTs), gene expression profiling and quantitative proteomics analysis, allowed identification of 262 unigenes (out of 3512) and 231 proteins (out of 1372 spots) that were differentially expressed along the stem. A good relationship was found between functional categories from transcriptomic and proteomic data. A good fit between the molecular mechanisms involved in CW-BW formation and these two types of wood phenotypic differences was also observed. This work provides a list of candidate genes for wood properties that will be tested in forward genetics.
Paiva
Jorge A P
JAP
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.|Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, 2780-157 Oeiras, Portugal.|Tropical Research Institute of Portugal (IICT), Forest and Forest Products Centre, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
Garcés
Marcelo
M
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.|Instituto de Biología Vegetal y Biotecnología. Universidad de Talca, Chile.
Alves
Ana
A
Tropical Research Institute of Portugal (IICT), Forest and Forest Products Centre, Tapada da Ajuda, 1349-017 Lisboa, Portugal.|Centro de Estudos Florestais, Departamento de Engenharia Florestal, Instituto Superior de Agronomia, ISA-DEF, Tapada Ajuda, 1349-017 Lisboa, Portugal.
Garnier-Géré
Pauline
P
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
Rodrigues
José Carlos
JC
Tropical Research Institute of Portugal (IICT), Forest and Forest Products Centre, Tapada da Ajuda, 1349-017 Lisboa, Portugal.|Centro de Estudos Florestais, Departamento de Engenharia Florestal, Instituto Superior de Agronomia, ISA-DEF, Tapada Ajuda, 1349-017 Lisboa, Portugal.
Lalanne
Céline
C
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
Porcon
Stéphane
S
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
Le Provost
Grégoire
G
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
Da Silva Perez
Denilson
D
FCBA InTechFibres, Laboratoire Bois Process, Domaine Universitaire, BP 251, 38044 Grenoble, Cedex 9, France.
Brach
Jean
J
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
Frigerio
Jean-Marc
JM
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
Claverol
Stéphane
S
Proteomic Facility, Université Bordeaux 2, 33076 Bordeaux, France.
Barré
Aurélien
A
Centre de Bioinformatique Bordeaux, Université Victor Segalen Bordeaux 2, rue Léo Saignat, 33076 Bordeaux Cedex, France.
Fevereiro
Pedro
P
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, 2780-157 Oeiras, Portugal.|Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1700 Lisboa, Portugal.
Plomion
Christophe
C
INRA, UMR 1202, Biodiversity Genes and Communities, 69 route d'Arcachon, F-33610 Cestas, France.
D000375:Aging; D017753:Ecosystem; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010641:Phenotype; D028223:Pinus / Q000737:chemistry* / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D020543:Proteome; D014934:Wood / Q000378:metabolism*; D052584:Xylem / Q000166:cytology / Q000378:metabolism
D010940:Plant Proteins; D020543:Proteome