title: Automatic positioning and sensing microelectrode array (APSMEA) for multi-site electrophysiological recordings.
abstract: Technological improvement of measurements for the electrical recordings from individual neurons within network is essential in neuroscience today. Here, we present a novel automatic positioning and sensing microelectrode array (APSMEA), which simultaneously positioned desired number of neurons onto 48 recording microelectrodes automatically and scathelessly by use of negative dielectrophoretic (DEP) forces, and facilitated the measurement of the electrophysiological activities of neuronal populations after functional synaptic connections formed between neurons. The results of multi-site electrophysiological recordings during drug administration also demonstrated the application of APSMEA in bioassay with cultured rat cortical neurons. Therefore, this device should benefit the investigation of neuronal networks in vitro with more comprehensive electrophysiological experiments, and also promise the possibility of a modular device for both cell manipulation and cell-based biosensor on microchip.
authors.affiliation: Medical Systems Biology Research Center, Tsinghua University, Beijing, China.
authors.forename: Liangbin
authors.initials: L
authors.lastname: Pan
authors.forename: Guangxin
authors.initials: G
authors.lastname: Xiang
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.forename: Zhongyao
authors.initials: Z
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.forename: Wanli
authors.initials: W
authors.lastname: Xing
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002478:Cells, Cultured; D004586:Electrophoresis; D004594:Electrophysiology / Q000295:instrumentation*; D008838:Microcomputers; D008839:Microelectrodes; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000502:physiology; D051381:Rats