title: [Effect of Bulbus Allii Macrostemi on gene expression profile associated with vascular endothelium injure of Qi stagnation rats].
abstract: OBJECTIVE
  To investigate the effect of Bulbus Allii Macrostemi on gene expression profile associated with inflammation and oxidative stress in vascular endothelium injure of qi stagnation rats.
  
  
  METHODS
  The model of vascular endothelium injure of qi stagnation rats were established by using high L-Methionine and being fastened. RT-PCR and serial analysis of gene expression (SAGE) database, available in NCBI, were used to analyze the changes of genes expression related to inflammation and oxidative stress in endothelium injure and the effect of Bulbus Allii Macrostemi.
  
  
  RESULTS
  Compared with model group, the genes expression of inflammation related COX-1 COX-2, oxidative stress related iNOS and eNOS, ECE involving in vascular vasomotion decreased (P < 0.05 or P < 0.01), but the gene expression of antioxidative SOD increased (P < 0.01) in Bulbus Allii Macrostemi group.
  
  
  CONCLUSIONS
  Bulbus Allii Macrostemi can adjust the disorder of the genes expression, related to vascular endothelium injure in Qi stagnation rats, protecting the vascular ehndothelium from injure.
authors.affiliation: Yiling Medical Research Institution of Hebei, Shijiazhuang 050035, China.
authors.forename: Yi-ling
authors.initials: YL
authors.lastname: Wu
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Jin-sheng
authors.initials: JS
authors.lastname: Qi
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Jia
authors.forename: Yan-ning
authors.initials: YN
authors.lastname: Li
mesh_terms: D000490:Allium / Q000737:chemistry*; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D019413:Qi; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics
chemical_list: D004365:Drugs, Chinese Herbal; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases; D013482:Superoxide Dismutase