[Decoloration and bioaugmentation on azo dye by immobilized genetically engineered strain].
Decoloration and bioaugmentation on azo dye are investigated by using immobilized genetically engineered strain Escherichia coli JM109 (pGEX-AZR) on marcroporous foam carriers. The kinetics of the acid red GR decolorization by the immobilized E. coli JM109 (pGEX-AZR) accords with Andrews model proved by our experiments, and the kinetic parameters, mu(max,c), K(c) and K(ic), are found to be 49.2 mg x (g x h)(-1), 710.43 mg x L(-1) and 681.62 mg x L(-1) respectively. For continuous operating in the anaerobic SBRs with 10% inoculation of Escherichia coli JM109 (pGEX-AZR) on marcroporous foam carriers for 32 d, both the tolerance to red GR concentration shock and the colorific removal in the bioaugmented anaerobic SBRs are higher than the control system, and the acid red GR decoloration rate reached 90%. Changes in microbial community have been detected by the RISA, in which the introduced immobilized GEM and preponderant mixed culture were subsisted steadily in sludge systems.
Jin
Ruo-Fei
RF
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China. jruofei@dlut.edu.cn
Zhou
Ji-Ti
JT
Wang
Jing
J
Cao
Tong-Chuan
TC
D000693:Anaerobiosis; D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology; D018914:Cells, Immobilized / Q000378:metabolism; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism*
D001391:Azo Compounds; D004396:Coloring Agents; D012235:Rhodamines; D014874:Water Pollutants, Chemical; C022027:lissamine rhodamine B