Low-cost-medium throughput Sanger dideoxy sequencing.
Serial analysis of gene expression (SAGE) requires the sequencing of DNA. The principal cost of SAGE is largely determined by the cost of sequencing. Therefore, it is important to have access to a robust and affordable sequencing system. Here, we describe such a system based on the sequencing of amplified inserts of concatemer-containing clones.
Nielsen
Kåre Lehmann
KL
Department of Life Sciences, University of Aalborg, Aalborg, Denmark.
D001483:Base Sequence; D002850:Chromatography, Gel; D003365:Costs and Cost Analysis; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D020869:Gene Expression Profiling / Q000191:economics / Q000379:methods*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000191:economics / Q000379:methods*
D017931:DNA Primers; D004247:DNA