Synthesis, GC selective DNA binding and topoisomerase II inhibition activities of ruthenium(II) polypyridyl complex containing 11-aminopteridino[6,7-f][1,10]phenanthrolin-13(12H)-one.
A DNA-intercalating Ru(II) polypyridyl complex [Ru(bpy)2(appo)]2+ (bpy=2,2'-bipyridine, appo=11-aminopteridino[6,7-f][1,10]phenanthrolin-13(12H)-one) has been synthesized and characterized by elemental analysis, electrospray mass spectra, (1)H NMR, UV/Vis spectrum, fluorescent spectrum and electrochemistry. The DNA-binding, photocleavage, and topoisomerase inhibition of the complex was studied. Interestingly, the complex binds to DNA via an intercalative mode with preference for GC sequences and cleaves the pBR322 DNA upon irradiation. In addition, the complex shows high inhibition activity against topoisomerase II by interfere the DNA religation.
Gao
Feng
F
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China.
Chao
Hui
H
Zhou
Feng
F
Chen
Xin
X
Wei
Yuan-Fang
YF
Ji
Liang-Nian
LN
D004247:DNA / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D006358:Hot Temperature; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D008163:Luminescent Measurements; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D059005:Topoisomerase II Inhibitors
D004791:Enzyme Inhibitors; D007364:Intercalating Agents; D009942:Organometallic Compounds; D059005:Topoisomerase II Inhibitors; C530401:bis(2,2'-bipyridine)(11-aminopteridine(6,7-f)(1,10)phenanthrolin-13(121H)-one)ruthenium(II); D004247:DNA