Trehalose is an important mediator of Cap1p oxidative stress response in Candida albicans.
Trehalose, a nonreducing disaccharide which accumulates dramatically during stationary phase or under oxidative stress, is well known as a stress protectant in several organisms. Here we investigated the putative correlation of trehalose with Cap1p, which is a basic region-leucine zipper (bZip) transcription factor participating in oxidative stress tolerance in Candida albicans. HPLC-MS analysis showed that trehalose did not accumulate in the cap1/cap1 mutant during stationary phase. When the mutant was exposed to high concentration of H2O2, trehalose accumulation was still not induced. Under both of the conditions above, the cap1/cap1 mutant showed high sensitivity to H2O2, and the cell viability was rather low. Furthermore, when exogenous trehalose was added to the culture of the cap1/cap1 mutant, the tolerance of this strain to oxidative stress was increased. Real time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcript levels of TPS2 and TPS3 were increased in the wild type strain compared to that in cap1/cap1 mutant when exposed to H2O2. These results indicated that trehalose accumulation is important to the oxidative stress tolerance mediated by Cap1p in C. albicans.
Cao
Yingying
Y
School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Wang
Yan
Y
Dai
BaoDi
B
Wang
Bin
B
Zhang
Hai
H
Zhu
ZhenYu
Z
Xu
YongGang
Y
Cao
YongBing
Y
Jiang
YuanYing
Y
Zhang
GuoQing
G
D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000502:physiology*; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D005838:Genotype; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014199:Trehalose / Q000378:metabolism / Q000502:physiology*
D050976:Basic-Leucine Zipper Transcription Factors; D005656:Fungal Proteins; D016877:Oxidants; D014199:Trehalose; D006861:Hydrogen Peroxide