title: Differential changes in pyridoxine 5'-phosphate oxidase immunoreactivity and protein levels in the somatosensory cortex and striatum of the ischemic gerbil brain.
abstract: Pyridoxal 5'-phosphate (PLP) is an important cofactor in a wide range of biochemical reactions, such as the metabolism of various amino acids, including GABA. PLP is synthesized by the oxidation of pyridoxine 5'-phosphate (PNP), which is catalyzed by PNP oxidase (PNPO). We observed the changes in cresyl violet-positive neurons, PNPO immunoreactivity and PNPO protein levels in the somatosensory cortex and striatum in gerbils after 5 min of transient forebrain ischemia. Cresyl violet-positive neurons showed condensed cytoplasm in the somatosensory cortex and lateral part of the striatum at 2 days after ischemia/reperfusion. PNPO immunoreactivity began to increase in neurons in layers III and V at 3 h after ischemia/reperfusion and this immunoreactivity was significantly increased at 12 h after ischemia/reperfusion. Thereafter, PNPO immunoreactivity decreased with time after ischemia/reperfusion. PNPO-immunoreactive neurons were only slightly detected in the lateral part of the striatum at 12 h after ischemia/reperfusion. In addition, the PNPO protein levels in both the somatosensory cortex and striatum homogenates peaked at 12 h after ischemia/reperfusion. These results indicate that PNPO is significantly increased in the ischemic somatosensory cortex and lateral part of the striatum, and this change in the level of PNPO may be associated with the neuronal damage induced by ischemia.
authors.affiliation: Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
authors.forename: In Koo
authors.initials: IK
authors.lastname: Hwang
authors.forename: Ki-Yeon
authors.initials: KY
authors.lastname: Yoo
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Soon Sung
authors.initials: SS
authors.lastname: Lim
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D048588:Benzoxazines; D015153:Blotting, Western; D004396:Coloring Agents; D003627:Data Interpretation, Statistical; D005849:Gerbillinae; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D017072:Neostriatum / Q000201:enzymology / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000473:pathology; D010078:Oxazines; D011734:Pyridoxaminephosphate Oxidase / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000201:enzymology / Q000378:metabolism*
chemical_list: D048588:Benzoxazines; D004396:Coloring Agents; D009419:Nerve Tissue Proteins; D010078:Oxazines; C028911:cresyl violet; D011734:Pyridoxaminephosphate Oxidase