The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil.
The production and properties of a rhamnolipid-type biosurfactant, synthesized by the Pseudomonas aeruginosa LBM10 strain, isolated from a southern coastal zone in Brazil, were investigated. The assays were conducted in a rotary shaker at 30 degrees C and 180 rpm for a period of 96 h. Soybean oil and sodium nitrate were the best sources of carbon and nitrogen, respectively. A nitrogen-limiting condition (C/N ratio of 100) was favorable to biosurfactant production. The formation of stable emulsions was better in saline concentrations below 0.5%, pH values in the range from 6 to 9 and temperatures in the range from 35 to 40 degrees C, maintaining about 80% of its original activity for salinity up to 3% and 120 min of exposure at 100 degrees C. The biosurfactant may be produced with this microorganism using renewable substrates that are readily available, reaching values of 1.42 g l(-1) measured as rhamnose. This biosurfactant has interesting and useful properties for many industrial applications.
Prieto
L M
LM
Bioprocess Engineering Laboratory, Federal University of Rio Grande, 96201-900 Rio Grande, RS, Brazil.
Michelon
M
M
Burkert
J F M
JF
Kalil
S J
SJ
Burkert
C A V
CA
D001938:Brazil; D002244:Carbon / Q000378:metabolism; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013696:Temperature
D004655:Emulsions; D008055:Lipids; D002244:Carbon; D009584:Nitrogen