[Preparation and in vitro tumor cells selectivity of sterically stabilized immunoliposomal peptides in bee venom].
Recently the use of peptides in bee venom (PBV) for cancer therapy has attracted considerable attention. In this study, the sterically stabilized liposomal PBV (PBV-SL) was prepared using soybean phosphatidylcholine, cholesterol, and cholesterol-PEG-COOH. The humanized antihepatoma disulfide-stabilized Fv (hdscFv25) was coupled to sterically stabilized liposomes using the N-hydroxysuccinimide ester method. The hdscFv25-immunoliposomes (SIL[hdscFv25]) were immunoreactive as determined by ELISA assay. SIL[hdscFv25] showed higher tumor cells selectivity. PBV-SIL[hdscFv25] can kill SMMC-7721 cells in vitro with higher efficiency than non-targeted liposomes. Whereas cytotoxicties were compared for Hela cells, no significant differences was observed between PBV-SIL[hdscFv25] and PBV-SL. Sterically stabilized immunoliposomal peptides in bee venom could be one drug targeting delivery system.
Hu
Hai-yang
HY
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. haiyang_hu@hotmail.com
Chen
Da-wei
DW
Liu
Yan-fang
YF
Qiao
Ming-xi
MX
Zhao
Xiu-li
XL
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology; D001514:Bee Venoms / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000737:chemistry; D016503:Drug Delivery Systems; D006367:HeLa Cells; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry / Q000494:pharmacology*; D008081:Liposomes / Q000737:chemistry; D008113:Liver Neoplasms / Q000473:pathology; D008555:Melitten / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010455:Peptides / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
D000970:Antineoplastic Agents; D001514:Bee Venoms; D018796:Immunoconjugates; D008081:Liposomes; D010455:Peptides; D011994:Recombinant Proteins; C513890:hdsFv25, human; D008555:Melitten; D002784:Cholesterol