title: Isolation and characterization of extracellular alpha-galactosidases from Penicillium canescens.
abstract: Two alpha-galactosidases were purified to homogeneity from the enzymatic complex of the mycelial fungus Penicillium canescens using chromatography on different sorbents. Substrate specificity, pH- and temperature optima of activity, stability under different pH and temperature conditions, and the influence of effectors on the catalytic properties of both enzymes were investigated. Genes aglA and aglC encoding alpha-galactosidases from P. canescens were isolated, and amino acid sequences of the proteins were predicted. In vitro feed testing (with soybean meal and soybean byproducts enriched with galactooligosaccharides as substrates) demonstrated that both alpha-galactosidases from P. canescens could be successfully used as feed additives. alpha-Galactosidase A belonging to the 27th glycosyl hydrolase family hydrolyzed galactopolysaccharides (galactomannans) and alpha-galactosidase C belonging to the 36th glycosyl hydrolase family hydrolyzed galactooligosaccharides (stachyose, raffinose, etc.) of soybean with good efficiency, thus improving the digestibility of fodder.
authors.affiliation: Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia. oasinitsyna@gmail.com
authors.forename: O A
authors.initials: OA
authors.lastname: Sinitsyna
authors.forename: E A
authors.initials: EA
authors.lastname: Fedorova
authors.forename: I M
authors.initials: IM
authors.lastname: Vakar
authors.forename: E G
authors.initials: EG
authors.lastname: Kondratieva
authors.forename: A M
authors.initials: AM
authors.lastname: Rozhkova
authors.forename: L M
authors.initials: LM
authors.lastname: Sokolova
authors.forename: T M
authors.initials: TM
authors.lastname: Bubnova
authors.forename: O N
authors.initials: ON
authors.lastname: Okunev
authors.forename: A M
authors.initials: AM
authors.lastname: Chulkin
authors.forename: Y P
authors.initials: YP
authors.lastname: Vinetsky
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D000821:Animal Feed; D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005690:Galactose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008670:Metals / Q000737:chemistry; D010407:Penicillium / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature; D000519:alpha-Galactosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D005656:Fungal Proteins; D008670:Metals; D000519:alpha-Galactosidase; D005690:Galactose