Primer binding site-dependent restriction of murine leukemia virus requires HP1 binding by TRIM28.
TRIM28 is a transcriptional corepressor which is required for primer binding site (PBS)-dependent restriction of murine leukemia virus (MLV) replication in embryonic stem and embryonic carcinoma (EC) cells. PBS-dependent restriction of MLV leads to transcriptional silencing of the integrated provirus and has been shown to correlate with TRIM28-mediated recruitment of HP1 to the silenced loci. Here we show, using a cell line with a point mutation in the HP1 binding domain of TRIM28, that interaction with HP1 is absolutely required for the PBS-dependent restriction of MLV in the F9 EC cell line.
Wolf
Daniel
D
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, HHSC 1310, 701 West 168th Street, New York, NY 10032, USA.
Cammas
Florence
F
Losson
Régine
R
Goff
Stephen P
SP
D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D054278:Embryonal Carcinoma Stem Cells / Q000821:virology; D053595:Embryonic Stem Cells / Q000821:virology; D009052:Leukemia Virus, Murine / Q000502:physiology*; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism; D017354:Point Mutation; D011485:Protein Binding / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism; D000076183:Tripartite Motif-Containing Protein 28; D014779:Virus Replication
D002868:Chromosomal Proteins, Non-Histone; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D000090266:Chromobox Protein Homolog 5; C103190:Trim28 protein, mouse; D000076183:Tripartite Motif-Containing Protein 28