Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a "density cushion".
This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a "density cushion". The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The "density cushion" method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.
Wilk
Anna
A
Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
Urbańska
Katarzyna
K
Woolley
David E
DE
Korohoda
Włodzimierz
W
D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002645:Chickens; D004586:Electrophoresis / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism; D012430:Ruthenium Red / Q000378:metabolism; D013499:Surface Properties
D007202:Indicators and Reagents; D012430:Ruthenium Red