Induction of apoptosis of human tumor cells by hybrid liposomes including docosahexaenoic acid.
Inhibitory effects of hybrid liposomes (HL) composed of L-alpha-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene(20) sorbitan monooleate (Tween 80) including polyunsaturated fatty acids on the growth of human tumor cells were examined in vitro. Remarkably high inhibitory effects of HL including docosahexaenoic acid (HL-DHA) on the growth of lung carcinoma (RERF-LC-OK), colon tumor (WiDr), and stomach tumor (MKN45) cells were obtained. The induction of apoptosis by HL-DHA was revealed on the basis of fluorescence microscopic and flow cytometric analyses.
Goto
Koichi
K
Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Tanaka
Yoshihiro
Y
Matsumoto
Yoko
Y
Ueoka
Ryuichi
R
D017209:Apoptosis / Q000502:physiology*; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000473:pathology*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000737:chemistry* / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000473:pathology*; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011136:Polysorbates / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D005231:Fatty Acids, Unsaturated; D008081:Liposomes; D011136:Polysorbates; D004281:Docosahexaenoic Acids; D004134:Dimyristoylphosphatidylcholine