Assay of bradykinin-related peptides in human body fluids using capillary electrophoresis with laser-induced fluorescence detection.
A CE with LIF detection was developed for separation and determination of bradykinin (BK)-related peptides, such as BK, kallidin (Kal), and neurokinin A (NKA). BK-related peptides were derivatized with FITC prior to CE-LIF analysis. Sodium borate 10 mmol/L at pH 9.5 was selected as derivatization media in order to get the high efficiency. Three peptides were baseline-separated within 10 min by using 110 mmol/L sodium borate-sodium hydroxide solution at pH 10.0 as the running buffer. Concentration detection limits (S/N = 3) for BK, Kal, and NKA were 0.08, 0.5, and 0.2 nmol/L, respectively. Meanwhile we have also developed a simple, quick, and sensitive large-volume sample stacking (LVSS) technique for CE-LIF detection of BK, Kal, and NKA. By using this stacking technique, the detection limits (S/N = 3) for BK, Kal, and NKA were 0.02, 0.05, and 0.04 nmol/L, respectively. This method has been applied to the assay of human saliva and cerebrospinal fluid with satisfactory results.
Chen
Ying
Y
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fuzhou, Fujian, PR China.
Xu
Liangjun
L
Lin
Jinming
J
Chen
Guonan
G
D001920:Bradykinin / Q000032:analysis* / Q000134:cerebrospinal fluid; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007609:Kallidin / Q000032:analysis* / Q000134:cerebrospinal fluid; D015288:Neurokinin A / Q000032:analysis* / Q000134:cerebrospinal fluid; D012463:Saliva / Q000737:chemistry
D007609:Kallidin; D015288:Neurokinin A; D001920:Bradykinin