title: Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis.
abstract: To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from patients suspected of having BU were analyzed. Both smear preparation methods and both staining methods were equivalent in any combination. Thus we recommend ZN stained smears of unground tissue for peripheral treatment centres.
authors.affiliation: Laboratoire de Référence des Mycobactéries, Cotonou, Bénin, and Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
authors.forename: Dissou
authors.initials: D
authors.lastname: Affolabi
authors.forename: Honoré
authors.initials: H
authors.lastname: Bankolé
authors.forename: Anthony
authors.initials: A
authors.lastname: Ablordey
authors.forename: Julie
authors.initials: J
authors.lastname: Hounnouga
authors.forename: Pierre
authors.initials: P
authors.lastname: Koutchakpo
authors.forename: Ghislain
authors.initials: G
authors.lastname: Sopoh
authors.forename: Julia
authors.initials: J
authors.lastname: Aguiar
authors.forename: Ange
authors.initials: A
authors.lastname: Dossou
authors.forename: Roch Christian
authors.initials: RC
authors.lastname: Johnson
authors.forename: Séverin
authors.initials: S
authors.lastname: Anagonou
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
mesh_terms: D001431:Bacteriological Techniques; D001576:Benzophenoneidum; D054312:Buruli Ulcer / Q000175:diagnosis* / Q000601:surgery*; D004396:Coloring Agents; D004251:DNA Transposable Elements; D006801:Humans; D008853:Microscopy / Q000379:methods*; D019911:Mycobacterium ulcerans / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D040521:Subcutaneous Tissue / Q000382:microbiology*
chemical_list: D004396:Coloring Agents; D004251:DNA Transposable Elements; D012394:Rosaniline Dyes; D001576:Benzophenoneidum; C006898:carbol-fuchsin solution