Impedimetric approach for quantifying low bacteria concentrations based on the changes produced in the electrode-solution interface during the pre-attachment stage.
This paper describes an approach for quantifying low concentrations of bacteria, particularly Escherichia coli, based on the measurement of the initial attachment of bacteria to platinum surfaces, using impedance spectroscopy. The value of the interface capacitance in the pre-attachment stage (before 1min of attachment) showed correlation with suspended concentration of bacteria from 10(1) to 10(7)CFUmL(-1) (colony forming units per mL). This method was found to be sensitive to the attachment time, to the applied potential and to the size of the counter electrode. The sensor lifetime was also evaluated.
Muñoz-Berbel
X
X
Centro Nacional de Microelectrónica (IMB-CSIC), Esfera UAB, Campus Univ. Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. xavier.munoz@cnm.es
Vigués
N
N
Jenkins
A T A
AT
Mas
J
J
Muñoz
F J
FJ
D001422:Bacterial Adhesion / Q000502:physiology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology; D008839:Microelectrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions
D012996:Solutions