title: Intra-operative testing for parathyroid hormone: the Central Laboratory option.
abstract: BACKGROUND
  Intra-operative PTH testing in the operating theatre has proven to be an accurate way to verify the removal of all pathological parathyroid tissue in primary hyperparathyroidism. Its limitation is the high cost. An alternative, more cost-effective procedure is proposed: intra-operative PTH dosage at the Central Laboratory.
  
  
  METHODS
  Fifty-four patients underwent parathyroidectomy with intraoperative dosage of PTH at the Central Laboratory. Three blood samples were taken from each patient: just after the induction of anesthesia, 5 and 10 min after parathyroidectomy. The samples were sent to the Central Laboratory and analysed simultaneously. The results were phoned back to the theatre. The procedure was considered effective when PTH drop was >/=50% from basal value, 10 min after parathyroidectomy.
  
  
  RESULTS
  98.1% of patients proved recovered (average follow- up 31.1 months). The procedure had 3 false negatives, 1 false positive, with sensitivity, specificity, accuracy, positive predictive value and negative predictive value of 94.0%, 75.0%, 92.6%, 97.9%, and 50.0%, respectively.
  
  
  CONCLUSIONS
  The main disadvantage of the presented procedure is the long waiting time. Nevertheless this time is the same as that required for results from intra-operative histological examination, the only alternative to determine surgery effectiveness in centres where portable instrumentation for intra-operative PTH dosage in the operating theatre is not available. The advantage of intra-operative PTH at the Central Laboratory is the very low cost. If results in terms of sensitivity, specificity, accuracy, and cost are taken into consideration, intra-operative dosage of PTH at the Central Laboratory may be deemed a viable alternative to the operating theatre.
authors.affiliation: Endocrine Surgery Unit, University of Milan, San Paolo Hospital, 20142 Milan, Italy. loredana.depasquale@ao-sanpaolo.it
authors.forename: L
authors.initials: L
authors.lastname: De Pasquale
authors.forename: D
authors.initials: D
authors.lastname: Gobatti
authors.forename: M L
authors.initials: ML
authors.lastname: Ravini
authors.forename: A
authors.initials: A
authors.lastname: Barassi
authors.forename: W
authors.initials: W
authors.lastname: Porreca
authors.forename: G V
authors.initials: GV
authors.lastname: Melzi d'Eril
authors.forename: A
authors.initials: A
authors.lastname: Bastagli
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007755:Laboratories, Hospital; D008297:Male; D008875:Middle Aged; D016343:Monitoring, Intraoperative / Q000379:methods*; D009873:Operating Rooms; D010281:Parathyroid Hormone / Q000032:analysis*; D016105:Parathyroidectomy / Q000379:methods; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: C470125:PTH protein, human; D010281:Parathyroid Hormone