title: Catechin gallates are NADP+-competitive inhibitors of glucose-6-phosphate dehydrogenase and other enzymes that employ NADP+ as a coenzyme.
abstract: Recent studies have shown that glucose-6-phosphate dehydrogenase (G6PD) is an effectual therapeutic target for metabolic disorders, including obesity and diabetes. In this study, we used in silico and conventional screening approaches to identify putative inhibitors of G6PD and found that gallated catechins (EGCG, GCG, ECG, CG), but not ungallated catechins (ECG, GC, EC, C), were NADP(+)-competitive inhibitors of G6PD and other enzymes that employ NADP(+) as a coenzyme, such as IDH and 6PGD.
authors.affiliation: R&D Center, AmorePacific Corporation, 314-1 Bora-Dong, Giheung-Gu, Yongin, Gyeonggi 446-729, Republic of Korea.
authors.forename: Eui Seok
authors.initials: ES
authors.lastname: Shin
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Park
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Shin
authors.forename: Dooho
authors.initials: D
authors.lastname: Cho
authors.forename: Si Young
authors.initials: SY
authors.lastname: Cho
authors.forename: Dong Wook
authors.initials: DW
authors.lastname: Shin
authors.forename: Mira
authors.initials: M
authors.lastname: Ham
authors.forename: Jae Bum
authors.initials: JB
authors.lastname: Kim
authors.forename: Tae Ryong
authors.initials: TR
authors.lastname: Lee
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003067:Coenzymes / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D051379:Mice; D015394:Molecular Structure; D009249:NADP / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D003067:Coenzymes; D009249:NADP; C417939:catechin gallate; D002392:Catechin; D005954:Glucosephosphate Dehydrogenase