title: nm23-H1 expression in non-Hodgkin and Hodgkin lymphomas.
abstract: We assessed for nm23-H1 expression in 262 lymphoid neoplasms including 191 B-cell non-Hodgkin lymphoma (NHL), 54 T-cell NHL, and 17 Hodgkin lymphoma (HL). We used a monoclonal anti-nm23-H1 antibody, routinely processed tissue, and immunohistochemical methods. We semiquantified the percentage of positive cells (0%, <25%, 25% to 75%, and >75%) and also estimated staining intensity (1 to 3+). Some percentage of nm23-H1 positive cells was detected in almost all types of NHL and HL, but T-cell NHL (87%) and HL (94.1%) more frequently had >75% positive cells than B-cell NHL (47.6%) (T- NHL vs. B-NHL, P<0.0001; HL vs. B-NHL, P=0.0011). High-intensity (3+) nm23-H1 staining was also more frequently observed in T-cell NHL (61.1%) and HL (76.5%) compared with B-cell NHL (43.5%) (T- NHL vs. B-NHL, P=0.013; HL vs. B-NHL, P=0.007). In most types of NHL and HL the nm23-H1 immunoreactivity was predominantly cytoplasmic. However, in plasma cell myeloma (PCM) nm23-H1 immunoreactivity was predominantly nuclear. In B-cell NHL, the percentage of nm23-H1-positive cells and the intensity of staining was not significantly different between various lymphoma types with the exception of PCM. We conclude that nm23-H1 is expressed in most types of B-cell and T-cell NHLs and HL, with a greater number of positive cells and higher staining intensity in T-cell NHL and HL, and PCM often being negative. The abundant intracellular expression of nm23-H1 suggests that serum levels of nm23-H1 are a reflection of tumor content. Unlike the conclusions of earlier studies, nm23-H1 expression in B-cell NHL was not significantly increased in clinically aggressive versus indolent neoplasms.
authors.affiliation: Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Sema
authors.initials: S
authors.lastname: Bircan
authors.forename: Kedar V
authors.initials: KV
authors.lastname: Inamdar
authors.forename: George Z
authors.initials: GZ
authors.lastname: Rassidakis
authors.forename: L J
authors.initials: LJ
authors.lastname: Medeiros
mesh_terms: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000097:blood; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006689:Hodgkin Disease / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D016393:Lymphoma, B-Cell / Q000201:enzymology* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000201:enzymology* / Q000473:pathology; D054778:NM23 Nucleoside Diphosphate Kinases / Q000096:biosynthesis*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases