title: Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus.
abstract: An integrated genetic linkage map of the medicinal and ornamental plant Catharanthus roseus, based on different types of molecular and morphological markers was constructed, using a F(2) population of 144 plants. The map defines 14 linkage groups (LGs) and consists of 131 marker loci, including 125 molecular DNA markers (76 RAPD, 3 RAPD combinations; 7 ISSR; 2 EST-SSR from Medicago truncatula and 37 other PCR based DNA markers), selected from a total of 472 primers or primer pairs, and six morphological markers (stem pigmentation, leaf lamina pigmentation and shape, leaf petiole and pod size, and petal colour). The total map length is 1131.9 cM (centiMorgans), giving an average map length and distance between two markers equal to 80.9 cM and 8.6 cM, respectively. The morphological markers/genes were found linked with nearest molecular or morphological markers at distances varying from 0.7 to 11.4 cM. Linkage was observed between the morphological markers concerned with lamina shape and petiole size of leaf on LG1 and leaf, stem and petiole pigmentation and pod size on LG8. This is the first genetic linkage map of C. roseus.
authors.affiliation: National Institute for Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, Post Box No. 10531, New Delhi 110 067, India.
authors.forename: Sarika
authors.initials: S
authors.lastname: Gupta
authors.forename: Sashi
authors.initials: S
authors.lastname: Pandey-Rai
authors.forename: Suchi
authors.initials: S
authors.lastname: Srivastava
authors.forename: Subhas Chandra
authors.initials: SC
authors.lastname: Naithani
authors.forename: Manoj
authors.initials: M
authors.lastname: Prasad
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
mesh_terms: D001483:Base Sequence; D030008:Catharanthus / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018598:Minisatellite Repeats; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D014748:Vinca Alkaloids / Q000378:metabolism
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers; D014748:Vinca Alkaloids