title: Distribution of dolichol in the serum and relationships between serum dolichol levels and various laboratory test values.
abstract: We examined the correlations between serum dolichol levels and laboratory test parameters in patients affected by disease, as well as the distribution of dolichol in sera from patients with hyperbetalipoproteinemia and hyperalphalipoproteinemia. Serum dolichol was evaluated by a reverse-phase HPLC method. After centrifugation, the serum dolichol found in healthy controls was mainly associated with medium-sized particles of the high-density lipoprotein (HDL) fraction. For patients with hyperbetalipoproteinemia, serum dolichol was also associated with the medium HDL fractions. However, for hyperalphalipoproteinemia patients the levels of large HDL and serum dolichol were increased, and serum dolichol was mainly associated with the large HDL fraction. On laboratory tests of components, the dolichol level was not correlated with the values for markers of the liver and biliary system, with the values of renal function markers, with creatine kinase activity, amylase activity or uric acid concentration, but was correlated with total cholesterol, HDL-cholesterol and apoA-I concentrations, and with lactate dehydrogenase (LDH) activity. These results suggest that serum dolichol exclusively localized in HDL, and in subpopulation, that in normocholesterolemia or hyperbeta-cholesterolemia is associated with HDL(3), which is small sized and high density HDL, however, that in hyperalphacholesterolemia is associated with HDL(2), which is large sized and lower density HDL.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Shiota
authors.forename: Kengo
authors.initials: K
authors.lastname: Kiyota
authors.forename: Teruaki
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Shojiro
authors.initials: S
authors.lastname: Kano
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Kawamura
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Matsushima
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Uchino
authors.forename: Fumie
authors.initials: F
authors.lastname: Hashimoto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Hayashi
mesh_terms: D001053:Apolipoproteins / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004286:Dolichols / Q000097:blood*; D005260:Female; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D014280:Triglycerides / Q000097:blood
chemical_list: D001053:Apolipoproteins; D004286:Dolichols; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides