title: Mining phenotypes for gene function prediction.
abstract: BACKGROUND
  Health and disease of organisms are reflected in their phenotypes. Often, a genetic component to a disease is discovered only after clearly defining its phenotype. In the past years, many technologies to systematically generate phenotypes in a high-throughput manner, such as RNA interference or gene knock-out, have been developed and used to decipher functions for genes. However, there have been relatively few efforts to make use of phenotype data beyond the single genotype-phenotype relationships.
  
  
  RESULTS
  We present results on a study where we use a large set of phenotype data - in textual form - to predict gene annotation. To this end, we use text clustering to group genes based on their phenotype descriptions. We show that these clusters correlate well with several indicators for biological coherence in gene groups, such as functional annotations from the Gene Ontology (GO) and protein-protein interactions. We exploit these clusters for predicting gene function by carrying over annotations from well-annotated genes to other, less-characterized genes in the same cluster. For a subset of groups selected by applying objective criteria, we can predict GO-term annotations from the biological process sub-ontology with up to 72.6% precision and 16.7% recall, as evaluated by cross-validation. We manually verified some of these clusters and found them to exhibit high biological coherence, e.g. a group containing all available antennal Drosophila odorant receptors despite inconsistent GO-annotations.
  
  
  CONCLUSIONS
  The intrinsic nature of phenotypes to visibly reflect genetic activity underlines their usefulness in inferring new gene functions. Thus, systematically analyzing these data on a large scale offers many possibilities for inferring functional annotation of genes. We show that text clustering can play an important role in this process.
authors.affiliation: Research Laboratories of Bayer Schering Pharma AG, Berlin, Germany. groth@informatik.hu-berlin.de
authors.forename: Philip
authors.initials: P
authors.lastname: Groth
authors.forename: Bertram
authors.initials: B
authors.lastname: Weiss
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Pohlenz
authors.forename: Ulf
authors.initials: U
authors.lastname: Leser
mesh_terms: D000465:Algorithms; D030562:Databases, Protein; D016247:Information Storage and Retrieval / Q000379:methods; D005810:Multigene Family / Q000502:physiology*; D009323:Natural Language Processing; D010641:Phenotype; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome / Q000145:classification* / Q000378:metabolism*
chemical_list: D020543:Proteome