Symmetric synaptic patterns between starburst amacrine cells and direction selective ganglion cells in the rabbit retina.
Inputs from starburst amacrine cells (SACs) to ON-OFF direction selective ganglion cells (DSGCs) in the rabbit retina are themselves directional. However, the synaptic asymmetry between SACs and DSGCs required for generating direction selectivity has been controversial. We investigated dendritic contacts and distribution of inhibitory synapses between SACs and their overlapped DSGCs. Double injection of SAC/DSGC pairs and quantitative analysis revealed no obvious asymmetry of dendritic contacts between SACs and DSGCs. Furthermore, examination of the inhibitory input pattern on the dendrites of DSGCs using antibodies against GABA(A) receptors also suggested an isotropic arrangement with the overlapping SACs in both the preferred and the null directions. Therefore, the presynaptic mechanism of direction selectivity upon DSGCs may not result from a simple asymmetric arrangement with overlapping SACs. Multiple layer interactions and sophisticated synaptic connections between SACs and DSGCs are necessary.
Chen
Yung-Cheng
YC
Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, 30013, Taiwan.
Chiao
Chuan-Chin
CC
D000200:Action Potentials / Q000502:physiology / Q000528:radiation effects; D025042:Amacrine Cells / Q000166:cytology* / Q000502:physiology; D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D002452:Cell Count; D005260:Female; D007211:Indoles; D007546:Isoquinolines / Q000378:metabolism; D008297:Male; D009433:Neural Inhibition / Q000502:physiology; D009949:Orientation / Q000502:physiology*; D010775:Photic Stimulation / Q000379:methods; D011817:Rabbits; D011963:Receptors, GABA-A / Q000378:metabolism; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology; D013569:Synapses / Q000502:physiology*; D014795:Visual Pathways
D007211:Indoles; D007546:Isoquinolines; D011963:Receptors, GABA-A; C070614:neurobiotin; C007293:DAPI; D001710:Biotin; C017475:lucifer yellow