[mRNA expression of extracellular matrix proteins of vocal fold polyps and Reinke edema].
OBJECTIVE
To investigate the pathogenic mechanism of vocal fold polyps and Reinke's edema.


METHODS
A reverse-transcriptase polymerase chain reaction (RT-PCR) technique was adopted, mRNA levels of 9 proteins were measured in 12 vocal fold polyps, 2 Reinke's edema and 5 normal vocal folds (from total laryngectomy).


RESULTS
The results showed that in the vocal fold polyps, mRNA levels of collagenase and fibromodulin descended and levels of fibronectin increased (P < 0.05). mRNA levels of lysyl oxidase and hyaluronic acid synthase 2 had no statistic difference between lesions and normal vocal folds (P > 0.05). mRNA express of tropoelastin exon, elastase and hyaluronidase was positive in part of lesion tissue and positive in all normal vocal folds. mRNA of procollagen I was negative in both groups. In the Reinke's edema, mRNA express of fibronectin was close to vocal fold polyps and mRNA express of fibromodulin was close to normal vocal folds.


CONCLUSIONS
It was speculated phonation trauma and vocal fold restoring to trauma played an important role in pathogenic mechanism. Fibromodulin and fibronectin were two components involved in the disorders.
Huang
Dong-Yan
DY
Department of Otorhinolaryngology Head and Neck Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
Yang
Wei-Yan
WY
Guo
Wei-Wei
WW
Han
Dong-Yi
DY
D000328:Adult; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005260:Female; D005353:Fibronectins / Q000235:genetics; D006801:Humans; D007819:Laryngeal Edema / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011127:Polyps / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics*; D014827:Vocal Cords
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D012333:RNA, Messenger