title: [Effect of Bushen Yiqi Hexue recipe on endometrial P21 expression of embryo implantation dysfunctional mice].
abstract: OBJECTIVE
  To observe the effect of Bushen Yiqi Hexue recipe on endometrial P21 and PCNA expression of embryo implantation dysfunctional mice.
  
  
  METHODS
  The expression of P21 protein was analyzed by means of immunoblot and immunohistochemical method. The expression of PCNA protein was analyzed by means of immunohistochemical method.
  
  
  RESULTS
  The expression and disappearance of P21 and PCNA protein in endometrium of embryo implantation dysfunctional mice lagged behind in a temporal and cell specific manner. Bushenyiqihexue recipe could improve the expression of P21 and PCNA protein in a temporal and cell specific manner, and then improve the decidual reaction of endometrium of embryo implantation dysfunctional mice.
  
  
  CONCLUSIONS
  Improving the expression of P21 and PCNA protein in endometrium and eventually regulating endometrium decidual reaction is one of the mechanisms of improved embryo implantaion by Bushen Yiqi Hexue recipe.
authors.affiliation: Institute of Integrated Traditional and Western Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Huang
authors.forename: Guang-Yin
authors.initials: GY
authors.lastname: Huang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Xu
authors.forename: Ming-Min
authors.initials: MM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D018809:Proliferating Cell Nuclear Antigen