Evolution of structure in gamma-class carbonic anhydrase and structurally related proteins.
Protein structure contains evolutionary information and it is more highly conserved than sequence. The evolution of structure in gamma-class carbonic anhydrase (gamma-CA) and its structurally related proteins (gammaCASRPs) were discussed. To obtain a reliable analysis, we defined a subset that contains all specificities and organisms as the nonredundant set using QR factorization based on the multiple structural alignment of the known crystallographic structures of gammaCASRPs with Q(H) as the structural homology measure. Then, we applied unweighted pair group method with arithmetic averages (UPGMA) to reconstruct structural phylogeny. We found that gamma-CA most likely arose through duplication events; the domain of gamma-CA underwent a process of alpha-helical content from amino-terminal end to carboxyl-terminal end of the left-handed beta-helix (LbetaH); the capacity of gamma-CA to bind Zn occurred early in evolution and only later included the ability to catalyze the reversible hydration of CO(2) efficiently for the occurrence of two loops involving Glu 62 and Glu 84, respectively, and a long helix at the carboxyl-terminal end of the LbetaH. In addition, the main conserved regions in these structures are in the structurally constrained residues of LbetaH domain, and the topology of the structural dendrogram can be rather easily understood in terms of functional diversification.
Fu
Xiang
X
Institute of Resource Biology & Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Yu
Long-Jiang
LJ
Mao-Teng
Li
L
Wei
Li
L
Wu
Chen
C
Yun-Feng
Ma
M
D000595:Amino Acid Sequence; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000235:genetics*; D018360:Crystallography, X-Ray; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D002256:Carbonic Anhydrases