[Effect of apoptosis and anti-tumor of vesicular stomatitis virus on tumor cells of nasopharyngeal carcinoma in vitro].
OBJECTIVE
To study the result of HNE-1 cancer cells lesion and HNE-1 cancer cells apoptosis caused by vesicular stomatitis virus in vitro.


METHODS
Firstly, HNE-1 cancer cells of human nasopharyngeal carcinoma were cultured in vitro. Secondly, tumor cells were treated with vesicular stomatitis virus (VSV) in different concentration (0.1 moi, 1.0 moi, 10.0 moi and 100.0 moi), while cells without treatment were used as blank assay. Finally, we observed the lesion of HNE-1 cells treated with VSV for 24 to 48 hours under invert microscope, compared with HNE-1 cells of blank assay. By measurement of MTT reduction assay and Hoechst 33258- staining under fluorescence microscope, the effect of VSV killing HNE-1 cancer cells and the induction of apoptosis by VSV in HNE-1 cancer cells were investigated in vitro.


RESULTS
Compared with blank assay, HNE-1 cancer cells of human nasopharyngeal carcinoma treated with VSV showed phenomenon of lesion under invert microscope. Followed the increase of concentration of VSV, cell survival rate of HNE-1 cancer cells decreased. The research methods of MTT reduction assay and Hoechst 33258 staining under fluorescence microscope defined the effect of VSV killing HNE-1 cancer cells and confirmed that VSV inducing apoptosis of HNE-1 cancer cells in vitro.


CONCLUSIONS
VSV not only exhibited the anti-tumor activity in HNE-1 cancer cells of human nasopharyngeal carcinoma but also showed the potential power of inducing apoptosis of HNE-1 cancer cells in vitro.
Feng
Yong
Y
Department of Otorhinolaryngology, People's Hospital of Sichuan, Chengdu, 610072, China. yongfeng33@sina.com
He
Gang
G
Wu
Yang
Y
Wen
Yanjun
Y
D017209:Apoptosis; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D050130:Oncolytic Virotherapy; D018116:Vesiculovirus