tmRNA abundance in Streptomyces aureofaciens, S. griseus and S. collinus under stress-inducing conditions.
tmRNA and protein SmpB are the main components required for rescue of stalled ribosomes incapable of properly elongating or terminating the polypeptide chain. We examined the tmRNA level and protein synthesis in Streptomyces aureofaciens, S. griseus and S. collinus synthesizing tetracycline, streptomycin and kirromycin, respectively, during various stress conditions. Downshift in temperature caused a decrease in protein synthesis but the level of tmRNA increased. Shift up in temperature induced decay of tmRNA in all strains and in S. collinus led to stimulation and in S. aureofaciens and S. griseus to inhibition of protein synthesis. At high NaCl concentrations protein synthesis was inhibited and tmRNA decayed. Shift in pH from 7.0 to 5.0 had no pronounced effect on the tmRNA level while upshift to pH 9.0 in S. collinus and S. aureofaciens caused inhibition of protein synthesis and decay of tmRNA in S. collinus. In contrast, protein synthesis and tmRNA level increased in S. griseus at the alkaline pH. Our data show that tmRNA abundance is important for survival of streptomycetes under certain unfavorable conditions.
Palecková
P
P
Institute of Alicrobiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Felsberg
J
J
Bobek
J
J
Mikulík
K
K
D015152:Blotting, Northern; D006863:Hydrogen-Ion Concentration; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012965:Sodium Chloride / Q000009:adverse effects; D013302:Streptomyces / Q000502:physiology*; D013304:Streptomyces aureofaciens / Q000502:physiology*; D013696:Temperature
D012329:RNA, Bacterial; D012333:RNA, Messenger; C096222:tmRNA; D012965:Sodium Chloride; D012343:RNA, Transfer