Oligomerization of the potato virus X 25-kD movement protein.
A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg2+-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.
Leshchiner
A D
AD
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
Minina
E A
EA
Rakitina
D V
DV
Vishnichenko
V K
VK
Solovyev
A G
AG
Morozov
S Yu
SY
Kalinina
N O
NO
D019281:Dimerization; D005976:Glutaral / Q000737:chemistry; D053830:Plant Viral Movement Proteins / Q000737:chemistry* / Q000378:metabolism; D017863:Potexvirus; D020798:Two-Hybrid System Techniques; D014461:Ultracentrifugation
D053830:Plant Viral Movement Proteins; D005976:Glutaral