[Experimental studies of effects of Wujiwan extracts in different compatibilities on motility of isolated colon in guinea pig].
OBJECTIVE
To investigate the effects of Wujiwan extracts in two different compatibility proportions (named the No. 1 and No. 2 respectively) on motility of isolated colon in guinea pig, and compare the therapeutic effects with each other.


METHODS
To observe the function of the No. 1 and No. 2 with different concentrations on colon contraction induced by acetylcholine (Ach) and inhibition induced by adrenalin (Ad) respectively, and calculate the EC, with the method of Bliss.


RESULTS
The No. 1 and No. 2 both could significantly inhibit the contraction of colon, and in 15 minutes, there was significant time-dependence and concentration-dependence, and EC50 of the No. 1 was lower than that of No. 2. However, but when the colonic was inhibited by Ad, at the time of 9 min and 15 min, the No. 1 at the level of 300 mg x L(-1), and at the time of 15 min, the No. 2 at the level of 30 mg x L(-1), could both excite the colon significantly (P < 0. 05).


CONCLUSIONS
Our experiments indicate that the No. 1 and No. 2 both can significantly inhibit the colon contraction in guinea pig, and the No. 1 is superior to the No. 2 to some extent. And at the same time, we also can conclude that the No. 1 and No. 2 have exciting effects on inhibitory colon. Meanwhile, the No. 1 and No. 2 each have its own advantages based on the common therapeutic effects.
Wang
Ya-Jie
YJ
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Dong
Yu
Y
Zhu
Xiao-Xin
XX
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003106:Colon / Q000187:drug effects* / Q000502:physiology; D039323:Coptis / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004837:Epinephrine / Q000494:pharmacology; D032086:Evodia / Q000737:chemistry; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D029596:Paeonia / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000109:Acetylcholine; D004837:Epinephrine