Antigenic profiling of a Chlamydia trachomatis gene-expression library.
The obligate intracellular bacterium Chlamydia trachomatis is the causative agent of sexually transmitted chlamydia infections. A panel of 116 recombinant C. trachomatis proteins was evaluated comparatively to characterize both cell-mediated and humoral immune responses in patients with confirmed C. trachomatis genital infection. The antigens identified were categorized as being recognized exclusively by T cells (CT004, CT043, CT184, CT509, and CT611), B cells (CT082, CT089, CT322, CT396, and CT681), or both T cells and B cells (CT110 and CT443). This grouping of C. trachomatis antigens was correlated to their predicted cellular localization. The comparative evaluation presented here indicates that T cell antigens are located in all bacterial compartments, whereas antibody targets are mainly localized to the outer membrane (P = .0013). Overall, we have identified 5 T cell antigens, 5 B cell antigens, and 2 T/B cell antigens that are potential components for a future chlamydia vaccine.
Follmann
Frank
F
Chlamydia Research, Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark.
Olsen
Anja Weinreich
AW
Jensen
Klaus Thorleif
KT
Hansen
Paul Robert
PR
Andersen
Peter
P
Theisen
Michael
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002690:Chlamydia Infections / Q000276:immunology* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008297:Male; D008875:Middle Aged; D013347:Subcellular Fractions / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial