title: Expression of nucleostemin in prostate cancer and its effect on the proliferation of PC-3 cells.
abstract: BACKGROUND
  Nucleostemin is essential for the proliferation and survival of stem and cancer cells, but it is unknown whether this newly identified molecule is involved in prostate cancer pathogenesis.
  
  
  METHODS
  Total RNA and protein were extracted from prostate cancer tissues and PC-3, LNCap and DU145 cell lines. The nucleostemin mRNA and protein expression were measured by RT-PCR and Western blot. Immunohistochemistry was also used to detect the nucleostemin protein expression in prostate cancer tissues and PC-3 cells. A nucleostemin specific, short hairpin RNA, expression plasmid was used to transfect PC-3 cells. The changes of nucleostemin gene were detected and the proliferative capacity of the cells was determined.
  
  
  RESULTS
  Nucleostemin was highly expressed in prostate cancer tissues and cell lines. Nucleostemin expression level in the silencer group PC-3 cells remarkably reduced. The proliferation rate of silencer group PC-3 cells decreased and the percentage of G1 stage cells increased. The neoplasm forming capacity in nude mice of the silencer group PC-3 cells decreased significantly.
  
  
  CONCLUSIONS
  Nucleostemin is highly expressed in prostate cancer tissues and cell lines. The proliferative capacity of PC-3 cells is remarkably reduced after silencing nucleostemin gene expression.
authors.affiliation: Department of Urology, Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin 300211, China.
authors.forename: Ran-lu
authors.initials: RL
authors.lastname: Liu
authors.forename: Zhi-hong
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Zhao
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Qi
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sheng-zhi
authors.initials: SZ
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019204:GTP-Binding Proteins; D006801:Humans; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins