title: Unique expression of a small IL-32 protein in the Jurkat leukemic T cell line.
abstract: Interleukin (IL)-32 was recently identified as a new cytokine which induces various proinflammatory cytokines in human monocytes and macrophages. Therefore, IL-32 has been primarily studied in inflammatory models such as rheumatoid arthritis and inflammatory bowel diseases. The regulation of endogenous IL-32 in other immune cells remains unknown. In the present study, we stimulated Jurkat T cells with phytohaemagglutinin (PHA) and phorbol myristate acetate (PMA) and examined IL-32 expression at both the mRNA and protein levels. All mRNAs of the four IL-32 isoforms and the 12-15 kDa IL-32 protein were independent of PHA and PMA stimulation, however a 9 kDa molecular weight IL-32 protein in the cell culture supernatant was induced by PHA and PMA after 16 h of stimulation. Compared to other human cell lines, the Jurkat cell line constitutively expressed a 12-15 kDa molecule of IL-32, which is smaller than the known IL-32 isoforms. We used IL-32 shRNA to examine the specificity of the 12-15 kDa molecule. Upon IL-32 shRNA transfection, the 12-15 kDa band was decreased specifically as compared to the control scrambled clone. Thus, the constitutive expression of IL-32 mRNA as well as the predominant production of a smaller sized IL-32 isoform in Jurkat cells may implicate a role for IL-32 in human T cell leukemia.
authors.affiliation: Department of Immunology, College of Medicine, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Na-Young
authors.initials: NY
authors.lastname: Ko
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Chang
authors.forename: Jun-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Nam-Wook
authors.initials: NW
authors.lastname: Kim
authors.forename: Young-Mi
authors.initials: YM
authors.lastname: Kim
authors.forename: Wahn-Soo
authors.initials: WS
authors.lastname: Choi
authors.forename: Ji-Da
authors.initials: JD
authors.lastname: Choi
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Bae
authors.forename: Jae-Woo
authors.initials: JW
authors.lastname: Hong
authors.forename: Jun
authors.initials: J
authors.lastname: Jaekal
authors.forename: Tania
authors.initials: T
authors.lastname: Azam
authors.forename: Erk
authors.initials: E
authors.lastname: Her
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000818:Animals; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D019169:Jurkat Cells; D053146:Myeloblastin / Q000378:metabolism; D010835:Phytohemagglutinins / Q000276:immunology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000276:immunology
chemical_list: C496971:IL32 protein, human; D007378:Interleukins; D010835:Phytohemagglutinins; D020033:Protein Isoforms; D012333:RNA, Messenger; D053146:Myeloblastin; D013755:Tetradecanoylphorbol Acetate