title: Quantitative trait loci for white blood cell numbers in swine.
abstract: Differential white blood cell counts are essential diagnostic parameters in veterinary practice but knowledge on the genetic architecture controlling variability of leucocyte numbers and relationships is sparse, especially in swine. Total leucocyte numbers (Leu) and the differential leucocyte counts, i.e. the fractions of lymphocytes (Lym), polymorphonuclear leucocytes [neutrophils (Neu), eosinophils (Eos) and basophils (Bas)] and monocytes (Mon) were measured in 139 F(2) pigs from a Meishan/Pietrain family, before and after challenge with the protozoan pathogen Sarcocystis miescheriana for genome-wide quantitative trait loci (QTL) analysis. After infection, the pigs passed through three stages representing acute disease, reconvalescence and chronic disease. Nine genome-wide significant and 29 putative, single QTL controlling leucocyte traits were identified on 15 chromosomes. Because leucocyte traits varied with health and disease status, QTL influencing the leucocyte phenotypes showed specific health/disease patterns. Regions on SSC1, 8 and 12 contained QTL for baseline leucocyte traits. Other QTL regions reached control on leucocyte traits only at distinct stages of the disease model. Two-thirds of the QTL have not been described before. Single QTL explained up to 19% of the phenotypic variance in the F(2) animals. Related traits were partly under common genetic influence. Our analysis confirms that leucocyte trait variation is associated with multiple chromosomal regions.
authors.affiliation: Department of Veterinary Clinical Sciences, University of Giessen, D-35392 Giessen, Germany. gerald.reiner@vetmed.uni-giessen.de
authors.forename: G
authors.initials: G
authors.lastname: Reiner
authors.forename: R
authors.initials: R
authors.lastname: Fischer
authors.forename: S
authors.initials: S
authors.lastname: Hepp
authors.forename: T
authors.initials: T
authors.lastname: Berge
authors.forename: F
authors.initials: F
authors.lastname: Köhler
authors.forename: H
authors.initials: H
authors.lastname: Willems
mesh_terms: D000208:Acute Disease; D000818:Animals; D002874:Chromosome Mapping; D002908:Chronic Disease; D003433:Crosses, Genetic; D005260:Female; D007958:Leukocyte Count; D008297:Male; D010272:Parasitic Diseases / Q000097:blood / Q000235:genetics; D040641:Quantitative Trait Loci; D013552:Swine / Q000235:genetics* / Q000502:physiology; D013553:Swine Diseases / Q000097:blood / Q000235:genetics* / Q000503:physiopathology