[Studies on characteristic fingerprints of fat-soluble components of Marsdenia tenacissima by GC].
OBJECTIVE
To study characteristic fingerprints of the fat-soluble components of different Marsdenia tenacissima samples by GC and GC-MS.


METHODS
The sample was split in the 280 degrees C injection port, with 10:1 split ratio, and separated on a fused silica capillary column (DB-5, 30 m x 0.25 mm I. D., 0.25 microm film). The temperature program was 70 degrees C for 5 min, 8 degrees C x min(-1) to 200 degrees C, then 5 degrees C x min-1 to 260 degrees C, 260 degrees C for 10 min. The high pure nitrogen was used as a carrier gas, The FID temperature was 300 degrees C.


RESULTS
There are 15 common peaks in the GC fingerprints of M. tenacissima and they are identified by GC-MS. The analyses on cluster and similarity degree of the finger the crude drugs from various habitats have been performed.


CONCLUSIONS
The method has good repeatability. The GC fingerprint combined with the HPLC fingerprint of M. tenacissim can be used to evaluate its quality integrally.
Zhao
Lu-Hua
LH
Key Laboratory of Drug Quality Control and Pharmacovilance Ministry of Education, Nanjing 210009, China. zhaoluhua@hotmail.com
Xiang
Bing-Ren
BR
Li
Xiao-Jing
XJ
Yang
Li-Li
LL
D002681:China; D002849:Chromatography, Gas / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D017753:Ecosystem; D008401:Gas Chromatography-Mass Spectrometry; D031101:Marsdenia / Q000737:chemistry* / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal