Quantitation of biotin-binding immunoglobulins G, A, and M in Human Sera Using F(ab')2anti-human immunoglobulin-coated microplates.
Biotin-binding IgG (B-IgG) in human sera was quantified using previously developed F(ab')(2)anti-human IgG-coated multiwell microplates (Muratsugu M. et al., 2003, Biol. Pharm. Bull., 26, 1605--1608). The levels of B-IgG in sera, however, were higher than those we predicted. In this study, we modified the assay using F(ab')2anti-human IgG-coated multiwell microplates and successfully quantified the levels of B-IgG in sera. The cause of the unpredicted results was discussed in the text. In addition, the levels of biotin-binding IgA (B-IgA) and IgM (B-IgM) in sera could be measured using F(ab')2anti-human IgA- or IgM-coated multiwell microplates. We quantified B-IgG, B-IgA, and B-IgM in sera from healthy specimens and patients with bronchial asthma, atopic dermatitis, epilepsy, and juvenile rheumatoid arthritis.
Muratsugu
Makoto
M
Bioanalytical Science Laboratory, Department of Clinical Nutrition, Osaka Prefecture University, Habikino, Osaka 583-8555, Japan. mmakoto@rehab.osakafu-u.ac.jp
Yazawa
Ayaka
A
Fujiwara
Sami
S
Nishida
Satsuki
S
Fukui
Toru
T
D019921:Biotinylation; D002352:Carrier Proteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D011971:Receptors, Immunologic / Q000276:immunology*; D012015:Reference Standards
D002352:Carrier Proteins; C052643:F(ab')2 receptor; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011971:Receptors, Immunologic; C017071:biotin-binding proteins