title: Establishment and characterization of a new cell line derived from human colorectal laterally spreading tumor.
abstract: OBJECTIVE
  To study the molecular mechanism of laterally spreading tumor (LST), a cell line [Laterally Spreading Tumor-Rectum 1 (LST-R1)] was derived and the characteristics of this cell line were investigated.
  
  
  METHODS
  A new cell line (LST-R1) originated from laterally spreading tumor was established. Properties of the cell line were characterized using scanning and transmission electron microscopy, immunohistochemistry method, cytogenetic analysis and nude mice xenograft experiments. In vitro invasion assay, cDNA microarray and Western blotting were used to compare the difference between the LST-R1 and other colorectal cancer cell lines derived from prudent colon cancer.
  
  
  RESULTS
  Our study demonstrated that both epithelial special antigen (ESA) and cytokeratin-20 (CK20) were expressed in LST-R1. The cells presented microvilli and tight junction with large nuclei. The karyotypic analysis showed hyperdiploid features with structural chromosome aberrations. The in vivo tumorigenicity was also demonstrated in nude mice xenograft experiments. The invasion assay suggested this cell line has a higher invasive ability. cDNA microarray and Western blotting show the loss of the expression of E-cadherin in LST-R1 cells.
  
  
  CONCLUSIONS
  We established and characterized a colorectal cancer cell line, LST-R1 and LST-R1 has an obvious malignant tendency, which maybe partially attributed to the changes of the expression of some adhesion molecules, such as E-cadherin. It is also a versatile tool for exploring the original and progressive mechanisms of laterally spreading tumor and the early colon cancer genesis.
authors.affiliation: Department of Gastroenterology, Nanfang Hospital, Nanfang Medical University, Tong He, Guangzhou 510520, Guangdong Province, China.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Zhou-Sheng
authors.initials: ZS
authors.lastname: Lai
authors.forename: Chung-Man
authors.initials: CM
authors.lastname: Yeung
authors.forename: Ji-De
authors.initials: JD
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Deng
authors.forename: Hoi-Yee
authors.initials: HY
authors.lastname: Li
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Han
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Marie Chia-mi
authors.initials: MC
authors.lastname: Lin
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000473:pathology*; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D014407:Tumor Cells, Cultured