RpoS induces expression of the Vibrio anguillarum quorum-sensing regulator VanT.
In vibrios, regulation of the Vibrio harveyi-like LuxR transcriptional activators occurs post-transcriptionally via small regulatory RNAs (sRNAs) that destabilize the luxR mRNA at a low cell population, eliminating expression of LuxR. Expression of the sRNAs is modulated by the vibrio quorum-sensing phosphorelay systems. However, vanT mRNA, which encodes a LuxR homologue in Vibrio anguillarum, is abundant at low and high cell density, indicating that VanT expression may be regulated via additional mechanisms. In this study, Western analyses showed that VanT was expressed throughout growth with a peak of expression during late exponential growth. VanO induced partial destabilization of vanT mRNA via activation of at least one Qrr sRNA. Interestingly, the sigma factor RpoS significantly stabilized vanT mRNA and induced VanT expression during late exponential growth. This induction was in part due to RpoS repressing expression of Hfq, an RNA chaperone. RpoS is not part of the quorum-sensing regulatory cascade since RpoS did not regulate expression or activity of VanO, and RpoS was not regulated by VanO or VanT. VanT and RpoS were needed for survival following UV irradiation and for pigment and metalloprotease production, suggesting that RpoS works with the quorum-sensing systems to modulate expression of VanT, which regulates survival and stress responses.
Weber
Barbara
B
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
Croxatto
Antony
A
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
Chen
Chang
C
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
Milton
Debra L
DL
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D035001:Host Factor 1 Protein / Q000096:biosynthesis; D045726:Metalloproteases / Q000096:biosynthesis; D050296:Microbial Viability; D008954:Models, Biological; D008969:Molecular Sequence Data; D010860:Pigments, Biological / Q000096:biosynthesis; D053038:Quorum Sensing / Q000502:physiology*; D012808:Sigma Factor / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis*; D014466:Ultraviolet Rays; D014733:Vibrio / Q000502:physiology* / Q000528:radiation effects
D001426:Bacterial Proteins; D035001:Host Factor 1 Protein; D010860:Pigments, Biological; D012808:Sigma Factor; D014157:Transcription Factors; C069567:sigma factor KatF protein, Bacteria; D045726:Metalloproteases