[Polymorphisms of FAS and FASL genes and susceptibility of silicosis].
OBJECTIVE
To explore the relationship between polymorphisms of FAS and FASL genes and genetic susceptibility of silicosis.


METHODS
A case-control study was conducted. The case group was 183 male patients with silicosis and the control group was 111 male silica-exposed but without silicosis miners. Data on total dust concentrations was collected to estimate cumulative total dust exposure (CTE) of each subject and each person's characteristics and work history were obtained from questionnaire. Polymerase chain reaction re-strained fragment length polymorphism technique (PCR-RFLP) was applied to detect the single nucleotide polymorphisms (SNPs) of FAS-1377, FAS-670 and FASL-844. Associations between polymorphisms and risk of silicosis and stages, interactions between polymorphisms, between polymorphisms and CTE and smoking and haplotypes were analyzed.


RESULTS
There were no differences in the FAS-1377, FAS-670 and FASL-844 genotypes between the case group and the control group (P > 0.05). No association was observed between FAS-1377, FAS-670 and FASL-844 polymorphisms and silicosis and stages (P > 0.05). The frequencies of FAS-1377G/-670G haplotype in the cases (9.6%) were higher than those in the controls (3.6%) (P < 0.05). No interactions between the polymorphisms of different genes, the gene polymorphism and the total accumulative total dust, the gene polymorphism and smoking were observed (P > 0.05).


CONCLUSIONS
FAS-1377, FAS-670 and FASL-844 polymorphisms are not susceptible factors of silicosis. The FAS-1377G/-670G haplotype might be a susceptibility marker of silicosis.
Wu
Fen
F
Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China.
Qu
Ya-bin
YB
Sun
Pin
P
Ji
Fang
F
Qiu
Yu-lan
YL
Miao
Wen-bin
WB
Wang
Wei
W
Jin
Ru-feng
RF
Xia
Zhao-lin
ZL
D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D053222:Fas Ligand Protein / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012829:Silicosis / Q000235:genetics*; D019014:fas Receptor / Q000235:genetics*
C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor