Cytoprotective effect of baicalein against peroxynitrite-induced toxicity in LLC-PK(1) cells.
The various reactions of peroxynitrite (ONOO(-)) with enzymes, macromolecules and lipids, have been shown to influence cellular functions and may be involved in the pathogenesis of various diseases. Therefore, targeting ONOO(-)-induced cytotoxicity is now accepted as a viable strategy to alleviate disease signs in numerous diseases. There is a growing interest in the antioxidant characteristics and use of dietary flavonoids in the management of ONOO(-)-related diseases. Baicalein, a major bioactive flavonoid constituent of Scutellaria baicalensis Georgi, possesses a multitude of pharmacological activities that have been associated with its antioxidant effects. In this study, we have investigated the ONOO(-) scavenging ability of baicalein and its protective effect on ONOO(-)-induced cell death in cultured LLC-PK(1) cells. Baicalein was able to scavenge ONOO(-) in a concentration-dependent manner, which was comparable to penicillamine. In addition, baicalein at the concentration range of 10-50 microM increased cell viability and decreased LDH leakage in 3-morpholinosydnonimine (SIN-1)-treated cells, reflecting its prevention of ONOO(-)-induced cytotoxicity. These results suggest that the protective effects of baicalein can be explained with respect to its ability to scavenge ONOO(-) and may be useful as a possible therapeutic strategy for the treatment of involved diseases.
Piao
Xiang-Lan
XL
Chinese Minority Traditional Medicine Center, College of Life and Environment Sciences, Central University for Nationalities, Beijing, China.
Cho
Eun Jung
EJ
Jang
Moon Hee
MH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D044950:Flavanones / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018374:LLC-PK1 Cells; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013552:Swine
D000975:Antioxidants; D004791:Enzyme Inhibitors; D044950:Flavanones; D016166:Free Radical Scavengers; D030421:Peroxynitrous Acid; C006680:baicalein; C002385:linsidomine; D008981:Molsidomine; D007770:L-Lactate Dehydrogenase