Biodegradable and pH-sensitive hydrogels for cell encapsulation and controlled drug release.
Hydrogels with pH-sensitive poly(acrylic acid) (PAAc) chains and biodegradable acryloyl-poly(-caprolactone)-2-hydroxylethyl methacrylate (AC-PCL-HEMA) chains were designed and synthesized. The morphology of hydrogel was observed by scanning electron microscopy. The degradation of the hydrogel in the presence of Pseudomonas lipase was studied. The in vitro release of bovine serum albumin from the hydrogel was investigated. Cytotoxicity study shows that the AC-PCL-HEMA/AAc copolymer exhibits good biocompatibility. Cell adhesion and migration into the hydrogel networks were evaluated by using different cell lines. The hydrogel with a lower cross-linking density and a larger pore size exhibited a better performance for cells migration.
Wu
De-Qun
DQ
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry,Wuhan University, Wuhan 430072, People's Republic of China.
Sun
Yun-Xia
YX
Xu
Xiao-Ding
XD
Cheng
Si-Xue
SX
Zhang
Xian-Zheng
XZ
Zhuo
Ren-Xi
RX
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D016956:Burkholderia cepacia / Q000201:enzymology; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel / Q000379:methods; D003432:Cross-Linking Reagents; D016503:Drug Delivery Systems; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; D012710:Serum Albumin, Bovine; D008049:Lipase