Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene.
Carbonic anhydrase III (CA3) is an abundant muscle protein characteristic of adult type-1, slow-twitch, muscle fibres. In order to further understand the functions of the porcine CA3 protein in muscle, the temporal and spatial distributions of its gene product were analysed and the association between the presence of specific polymorphisms and carcass traits in the pig was also examined. Real-time PCR revealed that the CA3 mRNA expression showed no differences with age in skeletal muscles from Yorkshire pigs at postnatal day-1, month-2, and month-4. We provide the first evidence that CA3 is differentially expressed in the skeletal muscle of Yorkshire and Meishan pig breeds. In addition, the whole pig genomic DNA sequence of CA3 was investigated and shown to contain seven exons and six introns. Comparative sequencing of the gene from three pig breeds revealed the existence of microsatellite SJ160 in intron 5 and microsatellite SJ158 and a novel microsatellite marker that includes a tandem repeat of (TC)(n) in intron 4. We also determined the allele number and frequencies of the three loci in seven pig breeds and found that they are low polymorphic microsatellite markers. Statistical analysis showed that the CA3 microsatellite polymorphism was associated with dressing percentage, internal fat rate, carcass length, rib number and backfat thickness in the pig.
Wu
Jian
J
Department of Basic Veterinary Medicine, College of Veterinary Medicine, Huazhong Agriculture University, Wuhan, 430070, PR China.
Zhou
Donghai
D
Deng
Changyan
C
Xiong
Yuanzhu
Y
Lei
Minggang
M
Li
Fenge
F
Jiang
Siwen
S
Zuo
Bo
B
Zheng
Rong
R
D000818:Animals; D001483:Base Sequence; D001947:Breeding; D024403:Carbonic Anhydrase III / Q000235:genetics*; D005786:Gene Expression Regulation; D005787:Gene Frequency; D016678:Genome; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*; D013997:Time Factors
D024403:Carbonic Anhydrase III