Catechin gallates are NADP+-competitive inhibitors of glucose-6-phosphate dehydrogenase and other enzymes that employ NADP+ as a coenzyme.
Recent studies have shown that glucose-6-phosphate dehydrogenase (G6PD) is an effectual therapeutic target for metabolic disorders, including obesity and diabetes. In this study, we used in silico and conventional screening approaches to identify putative inhibitors of G6PD and found that gallated catechins (EGCG, GCG, ECG, CG), but not ungallated catechins (ECG, GC, EC, C), were NADP(+)-competitive inhibitors of G6PD and other enzymes that employ NADP(+) as a coenzyme, such as IDH and 6PGD.
Shin
Eui Seok
ES
R&D Center, AmorePacific Corporation, 314-1 Bora-Dong, Giheung-Gu, Yongin, Gyeonggi 446-729, Republic of Korea.
Park
Jiyoung
J
Shin
Jae-Min
JM
Cho
Dooho
D
Cho
Si Young
SY
Shin
Dong Wook
DW
Ham
Mira
M
Kim
Jae Bum
JB
Lee
Tae Ryong
TR
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D003067:Coenzymes / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D051379:Mice; D015394:Molecular Structure; D009249:NADP / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003067:Coenzymes; D009249:NADP; C417939:catechin gallate; D002392:Catechin; D005954:Glucosephosphate Dehydrogenase