Estimation of L-dopa from Mucuna pruriens LINN and formulations containing M. pruriens by HPTLC method.
A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of L-dopa in Mucuna pruriens seed extract and its formulations. The method involves densitometric evaluation of L-dopa after resolving it by HPTLC on silica gel plates with n-butanol-acetic acid-water (4.0+1.0+1.0, v/v) as the mobile phase. Densitometric analysis of L-dopa was carried out in the absorbance mode at 280 nm. The relationship between the concentration of L-dopa and corresponding peak areas was found to be linear in the range of 100 to 1200 ng/spot. The method was validated for precision (inter and intraday), repeatability, and accuracy. Mean recovery was 100.30%. The relative standard deviation (RSD) values of the precision were found to be in the range 0.64-1.52%. In conclusion, the proposed TLC method was found to be precise, specific and accurate and can be used for identification and quantitative determination of L-dopa in herbal extract and its formulations.
Modi
Ketan Pravinbhai
KP
Department of Pharmacology, Shri B. M. Shah College of Pharmaceutical Education & Research, College Campus, Modasa-383315, India. ketan_modi11@rediffmail.com
Patel
Natvarlal Manilal
NM
Goyal
Ramesh Kishorilal
RK
D000978:Antiparkinson Agents / Q000008:administration & dosage / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002855:Chromatography, Thin Layer; D007980:Levodopa / Q000008:administration & dosage / Q000032:analysis* / Q000302:isolation & purification; D031299:Mucuna / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry
D000978:Antiparkinson Agents; D010936:Plant Extracts; D007980:Levodopa