Proinflammatory and oxidative stress markers in patients with periodontal disease.
OBJECTIVE
To evaluate the involvement of proinflammatory and oxidative stress markers in gingival tissue in individuals with chronic periodontitis.


METHODS
Eighteen subjects were divided in two groups: experimental (age 52.9+/-5.0) and control (age 51.1+/-9.6). The activities of enzymatic antioxidants such as catalase, glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase, nonenzymatic antioxidants: total glutathione and reduced glutathione, oxidized glutathione (GSSG), thiobarbituric acid reactive substances (TBARS), and myeloperoxidase activity (MPO) were evaluated in gingival tissues from interproximal sites. Statistical differences between groups were determined by independent Student t test and P<.05.


RESULTS
Individuals with periodontal disease exhibited a significant increase in the activities of MPO, GPx, GST, and also in TBARS and GSSG levels in gingival tissue compared to the control group (P<.05).


CONCLUSIONS
The results of the present work showed an important correlation between oxidative stress biomarkers and periodontal disease.
Borges
Ivan
I
Department of Nutrition, Federal University of Santa Catarina, 88040-970 Florianópolis, SC, Brazil.
Moreira
Emília Addison Machado
EA
Filho
Danilo Wilhem
DW
de Oliveira
Tiago Bittencourt
TB
da Silva
Marcelo Barreto Spirelle
MB
Fröde
Tânia Silvia
TS
D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005881:Gingiva / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010510:Periodontal Diseases / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances
D000975:Antioxidants; D015415:Biomarkers; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D009195:Peroxidase; D005978:Glutathione