title: Ultrasonic approach to enhance signal-to-noise in three dimensional microarray.
abstract: Three-dimensional polyacrylamide gel microarray (3-D gel microarray) has a strong adsorption for hybridzation probes with fluorescent label, resulting in high fluorescent background noise. The background noise has hampered the broad application of the microarray in scientific research. A low-frequency ultrasound of 40 kHz has been successfully employed to efficiently remove the nonspecifically bound fluorescent probes from the 3-D gel microarray. The method can significantly enhance the signal to noise ratio of the 3-D gel microarray and make it work better in single-nucleotide polymorphism genotyping.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Pan
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Li
authors.forename: Dongrui
authors.initials: D
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Minghao
authors.initials: M
authors.lastname: Zhang
authors.forename: Pengfeng
authors.initials: P
authors.lastname: Xiao
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000180:Acrylic Resins; D005838:Genotype; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results; D014465:Ultrasonics
chemical_list: D000180:Acrylic Resins; C016680:polyacrylamide gels