Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage.
A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.
Savalia
Dhruti
D
Waksman Institute for Microbiology, Piscataway, NJ 08854, USA.
Westblade
Lars F
LF
Goel
Manisha
M
Florens
Laurence
L
Kemp
Priscilla
P
Akulenko
Natalja
N
Pavlova
Olga
O
Padovan
Julio C
JC
Chait
Brian T
BT
Washburn
Michael P
MP
Ackermann
Hans-W
HW
Mushegian
Arcady
A
Gabisonia
Tarasii
T
Molineux
Ian
I
Severinov
Konstantin
K
D003090:Coliphages / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D023281:Genomics; D011401:Promoter Regions, Genetic; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
D020543:Proteome; D012321:DNA-Directed RNA Polymerases