title: Food safety evaluation of broccoli and radish sprouts.
abstract: Three cultivars of broccoli seeds (Brassica oleracea var. italica), cv. Tiburon, cv. Belstar and cv. Lucky, and two cultivars of radish seeds (Raphanus sativus), cv. Rebel and cv. Bolide, were germinated for three and five days and safety aspects such as microbiological counts and biogenic amines were investigated. Cytotoxicity evaluation was also carried out. Broccoli and radish sprouts contained numbers of mesophilic, psychrotrophic, total and faecal coliform bacteria which are the usual counts for minimally processed germinated seeds. Putrescine, cadaverine, histamine, tyramine, spermidine and spermine increased during sprout production although these levels were below those permitted by legislation (5 mg/100 g of edible food). Broccoli and radish sprouts demonstrated no toxic effects on proliferation and viability of HL-60 cells and should be included in our diets as healthy and safe fresh foods.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Villaluenga
authors.forename: Juana
authors.initials: J
authors.lastname: Frías
authors.forename: Piotr
authors.initials: P
authors.lastname: Gulewicz
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gulewicz
authors.forename: Concepción
authors.initials: C
authors.lastname: Vidal-Valverde
mesh_terms: D001679:Biogenic Amines / Q000032:analysis; D001937:Brassica / Q000382:microbiology* / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae; D018525:Germination; D018922:HL-60 Cells; D006801:Humans; D031224:Raphanus / Q000382:microbiology* / Q000633:toxicity*; D012639:Seeds / Q000737:chemistry
chemical_list: D001679:Biogenic Amines