Heterologous prime-boost immunizations with different Salmonella serovars for enhanced antigen-specific CD8 T-cell induction.
Pre-existing anti-vector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. In the present study, we demonstrate that heterologous prime-boost immunizations using attenuated serovar Typhimurium and serovar Dublin strains for foreign antigen delivery can be employed to bypass anti-Salmonella immunity resulting in enhanced antigen-specific CD8 T-cell induction. This desirable effect can be explained by the fact that, in contrast to homologous prime-boost immunizations, vaccination with different Salmonella serovars is characterized by long-lasting colonization of mice by both live carrier vaccines.
Sevil Domènech
Victòria E
VE
Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians-University Munich, Pettenkoferstrasse 9a, 80336 Munich, Bavaria, Germany.
Panthel
Klaus
K
Winter
Sebastian E
SE
Rüssmann
Holger
H
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002432:Cecum / Q000382:microbiology; D015169:Colony Count, Microbial; D005260:Female; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000096:biosynthesis; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000382:microbiology; D022562:Salmonella Vaccines / Q000276:immunology*; D019779:Salmonella enterica / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D022562:Salmonella Vaccines; D007371:Interferon-gamma