title: Effect of maturation on the composition and biological activity of the essential oil of a commercially important Satureja species from Turkey: Satureja cuneifolia Ten. (Lamiaceae).
abstract: The essential oil obtained by hydrodistillation from aerial parts of Satureja cuneifolia Ten., collected in three different maturation stages such as preflowering, flowering, and postflowering, were analyzed simultaneously by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Thymol (42.5-45.2%), p-cymene (19.4-24.3%), and carvacrol (8.5-13.2%) were identified as the main constituent in all stages. At the same time, the essential oils and main components were evaluated for their antimicrobial activity using a microdilution assay resulting in the inhibition of a number of common human pathogenic bacteria including methicillin-resistant Staphylococcus aureus (MRSA) and the yeasts Candida albicans and Candida tropicalis. The minimum inhibitory concentrations (MIC) varied between 62.5 and 250 microg/mL within a moderate antimicrobial activity range. Furthermore, the antioxidant capacity of the essential oils and major components thymol and carvacrol were examined in vitro. The essential oils obtained from S. cuneifolia in three different stages and its main components were interacted with 1,1-diphenyl-2-picrylhydrazyl (DPPH (*)) as a nitrogen-centered stable radical, resulting in IC 50 = 1.6-2.1 mg/mL. In addition, the effects on inhibition of lipid peroxidation of the essential oils were assayed using the beta-carotene bleaching method. All of the tested oils inhibited the linoleic acid peroxidation at almost the same level as butylated hydroxytoluene (BHT) (93.54-94.65%). BHT and ascorbic acid were used as positive controls in the antioxidant assays.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey. mkosar@anadolu.edu.tr
authors.forename: Müberra
authors.initials: M
authors.lastname: Koşar
authors.forename: Betül
authors.initials: B
authors.lastname: Demirci
authors.forename: Fatih
authors.initials: F
authors.lastname: Demirci
authors.forename: K Hüsnü Can
authors.initials: KH
authors.lastname: Başer
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D002849:Chromatography, Gas; D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D008401:Gas Chromatography-Mass Spectrometry; D039821:Monoterpenes / Q000032:analysis; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D031362:Satureja / Q000737:chemistry* / Q000254:growth & development*; D013943:Thymol / Q000032:analysis; D014421:Turkey
chemical_list: D000890:Anti-Infective Agents; D000081005:Cyclohexane Monoterpenes; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; C007210:4-cymene; D013943:Thymol; C018669:gamma-terpinene; C073316:carvacrol