[Effect of recipient mouse splenocytes taken orally by donor mouse on GVHD after splenocyte transplantation].
This study was aimed to explore whether the GVHD in mice can be ameliorated and the GVL effect in mice can be reserved by transfusion of lymphocytes of donors fed with recipient splenocytes effect. Male (DBA-2) mice (H-2(d)) as donors were fed with BALB/c splenocytes, DBA-2 splenocytes, bovine serum albumin, or regular chow, every other day. Induction of tolerance was assessed by a mixed lymphocyte reaction (MLR). Female (BALB/c) mice (H-2(d)) as recipients received total body irradiation (TBI) of 6.0 Gy ((60)Cogamma-ray) followed by inoculation of 3 x 10(3) P388 mouse leukemia cells on the same day. Subsequently, tail vein injection of 2 x 10(7) splenocytes supplied by DBA-2 was undertaken. Control groups were fed identically without leukemia cell inoculation. The results showed that GVHD was significantly ameliorated and CD4(+)/CD8(+) ratio increased in recipient-mice transplanted with splenocytes of tolerated donors, compared with control animals. There was no significant difference in survival rate between different groups of recipients inoculated with leukemia cell. It is concluded that the peroral recipient-mouse splenocytes can ameliorate GVHD without hampering effect on GVL.
Chen
Bao-An
BA
Department of Hematology, Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China. bachen@seu.edu.cn
Deng
Xiao-Jing
XJ
Ding
Jia-Hua
JH
Sun
Xue-Mei
XM
Dong
Wei-Min
WM
Bi
Yan-Zhi
YZ
Zhang
Yan
Y
Zhao
Gang
G
Gao
Chong
C
Sun
Yun-Yu
YY
Wang
Jun
J
Cheng
Jian
J
Ma
Yan
Y
Song
Hui-Hui
HH
Bao
Wen
W
Schmitt
A
A
Schmitt
M
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002457:Cell Extracts / Q000276:immunology / Q000494:pharmacology*; D017690:Cell Transplantation; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology / Q000517:prevention & control*; D020544:Graft vs Leukemia Effect / Q000276:immunology; D007941:Leukemia P388 / Q000628:therapy; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D014916:Whole-Body Irradiation
D000276:Adjuvants, Immunologic; D002457:Cell Extracts