In vivo mice lung tumor follow-up with fluorescence diffuse optical tomography.
We present in vivo experiments conducted with a new fluorescence diffuse optical tomographic (fDOT) system on cancerous mice bearing mammary murine tumors. We first briefly present this new system that has been developed and its associated reconstruction method. Its main specificity is its ability to reconstruct the fluorescence yield even in heterogeneous and highly attenuating body regions such as lungs and to enable mouse inspection without immersion in optical index matching liquid (Intralipid and ink). Some phantom experiments validate the performance of this new system for heterogeneous media inspection. Its use for a mice study is then related. It consists in the follow-up of the lungs at different stages of tumor development after injection of RAFT-(cRGD)4-Alexa700. As expected, the reconstructed fluorescence increases along with the tumor stage. These results validate the use of our system for biological studies of small animals.
Koenig
Anne
A
Commissariat à l'Energie Atomique, Lab. d'Electronique de Technologie de l'Information, MINATEC, 17 rue des Martyrs, 38054 Grenoble, Cedex 9, France. anne.koenig@cea.fr
Hervé
Lionel
L
Josserand
Véronique
V
Berger
Michel
M
Boutet
Jérôme
J
Da Silva
Anabela
A
Dinten
Jean-Marc
JM
Peltié
Philippe
P
Coll
Jean-Luc
JL
Rizo
Philippe
P
D000818:Animals; D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008325:Mammary Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods