[Study of differentation of HL-60 cells by induction of SPGL].
OBJECTIVE
To study the effect of differentiation of HL-60 cells by induction of SPGL.


METHODS
Cell differentiation was analyzed by using Wrigh-Giemsa staining to observe the morphology changes of cells with microscope, NBT reductant test, surface differentiation antigen (CD11b and CD14) test in HL-60 cells treated by different doses (1 x 10(-5) - 5 x 10(-4) ng/ml) of SPGL at the 3th day.


RESULTS
The morphological changes showed cell differentiation characteristics; NBT reductant was significantly increased, and the number of NBT positive cells were related to dose of SPGL (in dose-dependent manner); Expression of CD11b and CD14 increased obviously.


CONCLUSIONS
The results indicate that the effect of differentiation of HL-60 cells by induction of SPGL and it induces the cells to differentiate along the monocyte and granulocyte lineage.
Yang
Hua-zhong
HZ
Medical College of Hunan Normal University, Hunan Changsha 410006, China.
Zhang
Jian-hui
JH
Song
Kui
K
Sun
Xiao-juan
XJ
D039481:CD11b Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D019685:Rosales / Q000737:chemistry*; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology*
D039481:CD11b Antigen; D004365:Drugs, Chinese Herbal; D018950:Lipopolysaccharide Receptors; D012503:Saponins