title: Biosorption of nickel, chromium and zinc by MerP-expressing recombinant Escherichia coli.
abstract: Escherichia coli hosts able to over-express metal-binding proteins (MerP) originating from Gram-positive (Bacillus cereus RC607) and Gram-negative (Pseudomonas sp. K-62) bacterial strains were used to adsorb Ni(2+), Zn(2+) and Cr(3+) in aqueous solutions. The initial adsorption rate and adsorption capacity were determined to evaluate the performance of the biosorbents. With the expression of MerP protein, the metal adsorption capacity of the recombinant strains for Ni(2+), Zn(2+) and Cr(3+) significantly improved. The cells carrying Gram-positive merP gene (GB) adsorbed Zn(2+) and Cr(3+) at a capacity of 22.3 and 0.98 mmol/g biomass, which is 121% and 72% higher, respectively, over that of the MerP-free host cells. Adsorption capacity of the cells carrying Gram-negative merP gene (GP) also increased 144% and 126% for Zn(2+) and Cr(3+), respectively. Both recombinant strains also exhibited 24% and 5% enhancement in adsorption of Ni(2+) for GB and GP, respectively. The initial adsorption rate of the recombinant biosorbents was also higher than that of the MerP-free host, suggesting an increased metal-binding affinity with MerP expression. Severe cell damage on GB biosorbent was observed after Cr(3+) adsorption, probably due to the metal toxicity effect on the cells.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Kao
authors.forename: Chieh-Chen
authors.initials: CC
authors.lastname: Huang
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
mesh_terms: D000327:Adsorption; D001409:Bacillus cereus / Q000235:genetics; D001673:Biodegradation, Environmental; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D007220:Industrial Waste / Q000032:analysis; D019216:Metals, Heavy / Q000302:isolation & purification* / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013057:Spectrum Analysis; D013816:Thermodynamics; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins; D007220:Industrial Waste; C086060:MerP protein, Bacteria; D019216:Metals, Heavy; D011506:Proteins; D011994:Recombinant Proteins