title: [Studies on characteristic fingerprints of fat-soluble components of Marsdenia tenacissima by GC].
abstract: OBJECTIVE
  To study characteristic fingerprints of the fat-soluble components of different Marsdenia tenacissima samples by GC and GC-MS.
  
  
  METHODS
  The sample was split in the 280 degrees C injection port, with 10:1 split ratio, and separated on a fused silica capillary column (DB-5, 30 m x 0.25 mm I. D., 0.25 microm film). The temperature program was 70 degrees C for 5 min, 8 degrees C x min(-1) to 200 degrees C, then 5 degrees C x min-1 to 260 degrees C, 260 degrees C for 10 min. The high pure nitrogen was used as a carrier gas, The FID temperature was 300 degrees C.
  
  
  RESULTS
  There are 15 common peaks in the GC fingerprints of M. tenacissima and they are identified by GC-MS. The analyses on cluster and similarity degree of the finger the crude drugs from various habitats have been performed.
  
  
  CONCLUSIONS
  The method has good repeatability. The GC fingerprint combined with the HPLC fingerprint of M. tenacissim can be used to evaluate its quality integrally.
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovilance Ministry of Education, Nanjing 210009, China. zhaoluhua@hotmail.com
authors.forename: Lu-Hua
authors.initials: LH
authors.lastname: Zhao
authors.forename: Bing-Ren
authors.initials: BR
authors.lastname: Xiang
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Li
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yang
mesh_terms: D002681:China; D002849:Chromatography, Gas / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D017753:Ecosystem; D008401:Gas Chromatography-Mass Spectrometry; D031101:Marsdenia / Q000737:chemistry* / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal