title: Association of methylenetetrahydrofolate reductase (MTHFR-677 and MTHFR-1298) genetic polymorphisms with occlusive artery disease and deep venous thrombosis in Macedonians.
abstract: OBJECTIVE
  To analyze the association of methylenetetrahydrofolate reductase polymorphisms (MTHFR-677 and MTHFR-1298) with occlusive artery disease and deep venous thrombosis in Macedonians.
  
  
  METHODS
  We examined 83 healthy respondents, 76 patients with occlusive artery disease, and 67 patients with deep venous thrombosis. Blood samples were collected and DNA was isolated from peripheral blood leukocytes. Identification of MTHFR mutations was done with CVD StripAssay (ViennaLab, Labordiagnostika GmbH, Vienna, Austria) and the population genetics analysis package, PyPop, was used for the analysis. Pearson P values, crude odds ratio, and Wald's 95% confidence intervals were calculated.
  
  
  RESULTS
  The frequency of C alleles of MTHFR-677 was 0.575 in patients with deep venous thrombosis, 0.612 in patients with occlusive artery disease, and 0.645 in healthy participants. The frequency of T allele of MTHFR-677 was lower in healthy participants (0.355) than in patients with occlusive artery disease (0.388) and deep venous thrombosis (0.425). The frequency of A allele for MTHFR-1298 was 0.729 in healthy participants, 0.770 in patients with occlusive artery disease, and 0.746 in patients with deep venous thrombosis. The frequency of C allele of MTHFR-1298 was 0.271 in healthy participants, 0.230 in patients with occlusive artery disease, and 0.425 in patients with deep venous thrombosis. No association of MTHFR-677 and MTHFR-1289 polymorphisms with occlusive artery disease and deep venous thrombosis was found, except for the protective effect of MTHFR/CA:CC diplotype for occlusive artery disease.
  
  
  CONCLUSIONS
  We could not confirm a significant association of MTHFR-677 and MTHFR-1289 polymorphisms with occlusive artery disease or deep venous thrombosis in Macedonians, except for the protective effect of MTHFR/CA:CC diplotype against occlusive artery disease.
authors.affiliation: Institute of Hearth Diseases, University School of Medicine St. Kiril and Metodij, Skopje, Republic of Macedonia. mspiroski@yahoo.com
authors.forename: Igor
authors.initials: I
authors.lastname: Spiroski
authors.forename: Sashko
authors.initials: S
authors.lastname: Kedev
authors.forename: Slobodan
authors.initials: S
authors.lastname: Antov
authors.forename: Todor
authors.initials: T
authors.lastname: Arsov
authors.forename: Marija
authors.initials: M
authors.lastname: Krstevska
authors.forename: Sloboda
authors.initials: S
authors.lastname: Dzhekova-Stojkova
authors.forename: Stojanka
authors.initials: S
authors.lastname: Kostovska
authors.forename: Dejan
authors.initials: D
authors.lastname: Trajkov
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Petlichkovski
authors.forename: Ana
authors.initials: A
authors.lastname: Strezova
authors.forename: Olivija
authors.initials: O
authors.lastname: Efinska-Mladenovska
authors.forename: Mirko
authors.initials: M
authors.lastname: Spiroski
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D009154:Mutation; D011110:Polymorphism, Genetic; D018843:Republic of North Macedonia / Q000453:epidemiology; D020246:Venous Thrombosis / Q000453:epidemiology / Q000235:genetics*
chemical_list: D042965:Methylenetetrahydrofolate Reductase (NADPH2)