Polarization-interferometric surface-plasmon-resonance imaging system.
A two-dimensional phase-detection system for a surface-plasmon-resonance sensor is presented. The sensor utilizes polarization interferometry to detect phase differences between the s and p polarizations. We successfully detected a spatial phase-difference variation, resulting from the biomolecular interactions, of less than 1 x 1 mm(2). The phase stability demonstrated in the experimental results was approximately 0.09 degrees, and the corresponding change in the refractive index detection limit was approximately 4.3 x 10(-6). The common-optical-path configuration of the proposed method allowed us to reduce disturbances from ambient conditions. Furthermore, this method is capable of real-time array detection.
Lee
Ju-Yi
JY
Institute of Opto-Mechatronics Engineering and Department of Mechanical Engineering, National Central University, Chung-Li, Taiwan. juyilee@cc.ncu.edu.tw
Chou
Teng-Ko
TK
Shih
Hsueh-Ching
HC
D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D008857:Microscopy, Interference / Q000295:instrumentation* / Q000379:methods; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods