title: [Induced differentiation of rat hepatic oval cells in-vitro by combined hepatocyte growth factor and epidermal growth factor treatment].
abstract: OBJECTIVE
  To characterize the biologic featrues of hepatic oval cells and their protein expression profiles during induced differentiation in vitro.
  
  
  METHODS
  Rat hepatic oval cells were treated with epidermal growth factor (EGF) and hepatocyte growth factor (HGF) in vitro, followed by morphological and molecular marker assessment by electromicroscopy, immunocytochemistry, RT-PCR and protein expression chip technology.
  
  
  RESULTS
  Ten weeks after induction, the levels of GST-P mRNA and M2-PK mRNA were significantly reduced, whereas those of ALB and CK18 were elevated. Significant variations of expression was seen in 8 protein species during the course of the induced differentiation.
  
  
  CONCLUSIONS
  Combined EGF and HGF treatment in vitro induces cell differentiation of hepatic oval cells, a process in which 8 protein species may play some regulatory roles.
authors.affiliation: Department of Pathology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Xue
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Che
authors.forename: Hui-xi
authors.initials: HX
authors.lastname: Wu
authors.forename: Rui-de
authors.initials: RD
authors.lastname: Hu
mesh_terms: D000418:Albumins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000235:genetics; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D053538:Keratin-18 / Q000378:metabolism; D040081:Protein Array Analysis; D011770:Pyruvate Kinase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000418:Albumins; D053538:Keratin-18; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D005982:Glutathione Transferase; D011770:Pyruvate Kinase