Preparation of thyroid tumor cells in aspiration biopsies for aspiration biopsy nucleic acid diagnosis.
BACKGROUND
The relative expression level of trefoil factor 3 (TFF3) mRNA to galectin-3 (LGALS3) mRNA (T/G ratio) is a useful marker to distinguish thyroid follicular carcinomas from adenomas. However, because of the interference by the simultaneously aspirated peripheral blood cells or infiltrating lymphocytes, the precise measurement of the T/G ratio in aspirates is difficult.


METHODS
We tested 2 methods of selecting thyroid tumor cells and removing blood cells from the aspirates. One method was selection with magnetic beads coated with Ber-EP4 antibody and the other was filtration through a nylon mesh.


RESULTS
After selection with Ber-EP4 antibody, T/G ratios in aspirates showed varied degrees of difference from those in the corresponding tissues. After mesh filtration, differences in T/G ratios between the aspirates and the corresponding tissues were less than 200% in all 8 cases.


CONCLUSIONS
Mesh filtration of aspirates proved superior results for the measurement of T/G ratio.
Takano
Toru
T
Department of Laboratory Medicine, Osaka University Graduate School of Medicine, D2, 2-2 Yamadaoka, Osaka 565-0871, Suita, Japan. ttakano@labo.med.osaka-u.ac.jp
Higashiyama
Takuya
T
Uruno
Takashi
T
Yamada
Hiroya
H
Yoshida
Hiroshi
H
Miyauchi
Akira
A
D000236:Adenoma / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000276:immunology; D044963:Biopsy, Fine-Needle; D002469:Cell Separation; D005374:Filtration / Q000295:instrumentation; D037502:Galectin 3 / Q000378:metabolism*; D006801:Humans; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D013961:Thyroid Gland / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology; D000071165:Trefoil Factor-3; D000951:Antigens, Neoplasm
D014408:Biomarkers, Tumor; D037502:Galectin 3; D017493:Leukocyte Common Antigens; D010455:Peptides; D012333:RNA, Messenger; D000071165:Trefoil Factor-3; C082495:TFF3 protein, human; C115012:human epithelial antigen-125; D000951:Antigens, Neoplasm