title: Genotypic, phenotypic, and clinical characteristics of isolates of Helicobacter pylori from San Luis, Argentina.
abstract: The vacA and cagA genotypes of Helicobacter pylori exhibited distinct geographic distribution and correlation with severity of disease. In the above genotypes (obtained from 150 H. pylori-positive patients--139 with gastritis, 10 with ulcer and 1 patient with gastric cancer) combinations vacA s1/m1 and s2/m2 were detected using PCR in 75 and 25% of isolates, respectively, in patients with chronic gastritis. The of s1/m1 and s2/m2 combinations were also detected from ulcers (60 and 40%, respectively). The cagA was detected in 30% of isolates. Concentrated culture supernatants of 7 (64%) out of 11 H. pylori strains induced vacuolization in Vero cells in titers ranging from 1:5 to 1:40. The vacA s1 genotype was significantly associated with, but not predictive of the presence of vacuolating cytotoxin activity and the cagA gene.
authors.affiliation: Area Microbiología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina. cmmattan@unsl.edu.ar
authors.forename: C M
authors.initials: CM
authors.lastname: Mattana
authors.forename: A E
authors.initials: AE
authors.lastname: Vega
authors.forename: F
authors.initials: F
authors.lastname: Escobar
authors.forename: L
authors.initials: L
authors.lastname: Sabini
authors.forename: S
authors.initials: S
authors.lastname: Zanón
authors.forename: O N
authors.initials: ON
authors.lastname: Puig de Centorbia
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000235:genetics; D001118:Argentina / Q000453:epidemiology; D001426:Bacterial Proteins / Q000235:genetics; D003603:Cytotoxins / Q000096:biosynthesis; D005773:Gastroscopy; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D013272:Stomach Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003603:Cytotoxins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori