title: Secretion of celiac disease autoantibodies after in vitro gliadin challenge is dependent on small-bowel mucosal transglutaminase 2-specific IgA deposits.
abstract: BACKGROUND
  In celiac disease gluten, the disease-inducing toxic component in wheat, induces the secretion of autoantibodies which are targeted against transglutaminase 2 (TG2). These autoantibodies are produced in the small-intestinal mucosa, where they can be found deposited extracellularly below the epithelial basement membrane and around mucosal blood vessels. In addition, during gluten consumption these autoantibodies can also be detected in patients' serum but disappear from the circulation on a gluten-free diet. Interestingly, after adoption of a gluten-free diet the serum autoantibodies disappear from the circulation more rapidly than the small-intestinal mucosal autoantibody deposits. The toxicity of gluten and the secretion of the disease-specific autoantibodies have been widely studied in organ culture of small-intestinal biopsy samples, but results hitherto have been contradictory. Since the mucosal autoantibodies disappear slowly after a gluten-free diet, our aim was to establish whether autoantibody secretion to organ culture supernatants in treated celiac disease patient biopsies is related to the duration of the diet and further to the pre-existence of mucosal TG2-specific IgA deposits in the cultured biopsy samples.
  
  
  RESULTS
  In the organ culture system conducted with biopsies derived from treated celiac disease patients, gliadin induced secretion of autoantibodies to culture supernatants, reduced epithelial cell height and increased the density of lamina proprial CD25+ cells. However, these changes could be demonstrated only in biopsies from short-term treated celiac disease patients, where the small-intestinal mucosal TG2-specific IgA autoantibody deposits were still present. Furthermore, in these biopsies autoantibody secretion could be stimulated fully only after a 48-hour gliadin challenge.
  
  
  CONCLUSIONS
  Our results show that studies focusing on the toxic effects of gliadin in the organ culture system should be carried out with biopsy samples from short-term treated celiac disease patients who are likely still to have mucosal IgA deposits present. In addition to providing an explanation for the discrepancies in previous publications, the present study also enables further validation of the organ culture method.
authors.affiliation: Medical School, Pediatric Research Center, University of Tampere, Tampere, Finland. satumarja.stenman@uta.fi
authors.forename: Satumarja M
authors.initials: SM
authors.lastname: Stenman
authors.forename: Katri
authors.initials: K
authors.lastname: Lindfors
authors.forename: Ilma R
authors.initials: IR
authors.lastname: Korponay-Szabo
authors.forename: Olli
authors.initials: O
authors.lastname: Lohi
authors.forename: Päivi
authors.initials: P
authors.lastname: Saavalainen
authors.forename: Jukka
authors.initials: J
authors.lastname: Partanen
authors.forename: Katri
authors.initials: K
authors.lastname: Haimila
authors.forename: Herbert
authors.initials: H
authors.lastname: Wieser
authors.forename: Markku
authors.initials: M
authors.lastname: Mäki
authors.forename: Katri
authors.initials: K
authors.lastname: Kaukinen
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000276:immunology* / Q000378:metabolism; D016022:Case-Control Studies; D002446:Celiac Disease / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D002452:Cell Count; D020895:Enterocytes / Q000187:drug effects / Q000473:pathology; D005260:Female; D019204:GTP-Binding Proteins / Q000276:immunology*; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques; D009928:Organ Specificity / Q000187:drug effects; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007070:Immunoglobulin A; D053645:Interleukin-2 Receptor alpha Subunit; D005903:Gliadin; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins