[Diagnostic value for detection of aspergillus ribosomal RNA in fungal rhinosinusitis with hybridization in situ].
OBJECTIVE
To develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis.


METHODS
Thirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementary to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species.


RESULTS
In situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23).


CONCLUSIONS
In situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed.
Li
Yuan
Y
Department of Otorhinolaryngology, Beijing China-Japan Friendship Hospital, Beijing 100029, China. yuanli0702@263.net
Liu
Yang
Y
Wang
Na-Ya
NY
Yang
Da-Zhang
DZ
Zhang
Qiu-Hang
QH
Wang
Yue
Y
Luo
Jie
J
D000328:Adult; D000368:Aged; D001228:Aspergillosis / Q000175:diagnosis*; D001230:Aspergillus / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D012331:RNA, Fungal / Q000302:isolation & purification; D012337:RNA, Ribosomal, 18S / Q000302:isolation & purification; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology*
D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S