Characterization of glycoside residues of porcine zona pellucida and ooplasm during follicular development and atresia.
The glycoside residues (glycoconjugates, GC) of the zona pellucida (ZP) glycoproteins are important during the first phases of fecundation. Our aim in this work was to determine the lectin affinity pattern of porcine ZP in order to analyze the changes that take place during: (a) preantral folliculogenesis, (b) the follicular atresia process, and (c) antral growth. Several prepubertal and adult pig ovaries and different sized antral follicles were used. Conventional carbohydrate histochemical techniques and peroxidase and digoxigenin (DIG) lectins were used to reveal the acid groups and the glycosidic residues of the ZP. It was seen that the ZP forms in the preantral follicles throughout their growth period. In primordial and primary follicles, ZP in the process of formation showed neutral GC. SBA, RCA-I, MAA, WGA lectins, and AAA after methylation-saponification (MS) were positive in the ZP of primordial and primary follicles. The affinity for SBA, RCA-I, MAA, and WGA increased in the multilaminar-primary follicles and new affinities for UEA-I and LFA were observed. After MS, AAA, SNA, PNA, and SBA reactivity was observed. The ZP of antral follicle oocytes of different sizes showed the same lectin pattern as multilaminar-primary follicles. The oocyte ooplasm and the follicular fluid of large antral follicles showed less affinity for WGA and LFA lectins and less intensive staining with AB (pH 2.5). Atresia did not change the antral or preantral follicle oocyte ZP lectin pattern. In conclusion, the follicles showed substantial changes in their ZP glycosidic composition as they developed, especially, during the change from primary to multilaminar-primary follicles. The ZP glycosidic composition showed no significant change during the growth of antral follicles and follicular atresia in our study.
Pastor
Luis M
LM
Department of Cellular Biology and Histology, Medical School, University of Murcia, Murcia, Spain. bioetica@um.es
Lucas
Xiomara
X
Pallares
Jacinto
J
Bernal-Mañas
Carmen M
CM
Martinez
Emilio A
EA
Roca
Jordi
J
Vazquez
Juan Maria
JM
Morales
Eva
E
Beltran
Esther
E
Zuasti
Adelina
A
Ferrer
Concepcion
C
D000818:Animals; D048429:Cell Size; D003593:Cytoplasm / Q000737:chemistry*; D005260:Female; D005496:Follicular Atresia / Q000502:physiology*; D006027:Glycosides / Q000032:analysis*; D006651:Histocytochemistry; D037102:Lectins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009865:Oocytes / Q000737:chemistry* / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D034421:Sus scrofa / Q000502:physiology; D015044:Zona Pellucida / Q000737:chemistry* / Q000378:metabolism
D006027:Glycosides; D037102:Lectins