Genetic analysis of betanodaviruses in subclinically infected aquarium fish and invertebrates.
Betanodaviruses causing viral nervous necrosis (VNN) have been detected and isolated from several species of cultured marine fish worldwide. In Korea, VNN was identified in several species of cultured marine fish. This study presents data on the amplified nested PCR product (420 bp) of 11 nodavirus strains from different species of apparently healthy aquarium fish and invertebrates collected in one private commercial aquarium in Korea. Phylogenetic analyses based on the partial nucleotide sequence (177 bases) of the RNA2 coat protein gene were identical to the redspotted grouper nervous necrosis virus (RGNNV) genotype (96%-100%). The presence of the RGNNV type of betanodaviruses in these subclinically infected aquarium fish and invertebrates imported from different countries probably indicates that the samples were contaminated inside the aquarium and represents a serious challenge for its management of viral nervous necrosis. These positive samples can be an inoculum source of betanodavirus infection to other susceptible fish species inside the aquarium.
Gomez
Dennis K
DK
KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul 151-742, Korea.
Baeck
Gun Wook
GW
Kim
Ji Hyung
JH
Choresca
Casiano H
CH
Park
Se Chang
SC
D000818:Animals; D017756:Aquaculture; D036022:Capsid Proteins / Q000145:classification / Q000235:genetics; D002353:Carrier State / Q000821:virology*; D005393:Fish Diseases / Q000821:virology*; D005399:Fishes / Q000821:virology; D007448:Invertebrates / Q000821:virology; D030381:Nodaviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D010802:Phylogeny; D012327:RNA Virus Infections / Q000145:classification / Q000235:genetics*
D036022:Capsid Proteins