Effects of inulin/oligofructose on the thermal stability and acid-induced gelation of soy proteins.
Differential scanning calorimetry (DSC) and dynamic oscillatory shear testing were performed to study the influence of inulin (Raftiline HP-gel and Raftiline ST-gel) and oligofructose (Raftilose P95) on the thermal stability and gelation (using glucono-delta-lactone [GDL] as a coagulant) of soy protein isolate (SPI) dispersions. Addition of 10% (w/v) inulin/oligofructose or sucrose increased (P < 0.05) the peak denaturation temperatures (T(m)) of 7S and 11S soy proteins in SPI dispersion (5%[w/v], pH 7.0) by an average of 1.9 and 2.3 degrees C, respectively. GDL induced SPI thermal gelation, and the gel rheology was affected by both the pH decline and the specific temperature of heating. Addition of inulin/oligofructose (8%, w/v) improved the gelling properties of preheated SPI dispersion (8%, w/v) coagulated with GDL, showing 14.4 to 45.6% increase (P < 0.05) in gel rigidity (G' value) at the end of heating (81 degrees C). Microstructural examination revealed a denser protein cross-linking structure and reduced pore sizes in SPI gels containing inulin/oligofructose. In general, inulin was more capable of improving SPI gelation than oligofructose, suggesting that the degree of fructose polymerization in the fructans was of thermal and rheological importance.
Tseng
Y-C
YC
Dept. of Animal and Food Sciences, Univ. of Kentucky, Lexington, KY 40546, USA.
Xiong
Y L
YL
Boatright
W L
WL
D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005511:Food Handling / Q000379:methods; D005524:Food Technology; D005782:Gels; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000737:chemistry / Q000494:pharmacology*; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D011489:Protein Denaturation; D012212:Rheology / Q000187:drug effects; D030262:Soybean Proteins / Q000737:chemistry*; D013997:Time Factors
D005782:Gels; D009844:Oligosaccharides; D030262:Soybean Proteins; C120489:oligofructose; D007444:Inulin