Differential expression of the carcinoembryonic antigen-related cell adhesion molecules panCD66, CD66a, CD66c and of sialyl-Lewis x (CD15s) on blast cells of acute leukemias.
Expression of CD66 has been reported to occur on blast cells from children with acute lymphoblastic leukemia (ALL), but little is known about the differential expression pattern of panCD66 and other members of the CD66 family on blast cells from patients with acute myeloid leukemia (AML). We have performed flow cytometry immunophenotyping on blast cells from 28 patients with acute myeloid leukemia (AML), 13 patients with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) and 7 patients with T-ALL using monoclonal antibodies (mAbs) against panCD66 (clone D14HD11), CD66a (clone 4.3.17), CD66c (clone 9A6) and CD15s. Expression of the panCD66 mAb was found to be positive in 13 of 28 patients with AML (46%) and in 6 of 13 patients with BCP-ALL (46%) but negative for all the seven patients with T-ALL. In AML, panCD66, CD66a and CD66c were more frequently coexpressed with CD65, CD15 and CD64 than with CD13, CD33 or the two progenitor markers CD34 and CD117. In contrast to CD15, the expression of the sialylated Lewis X (CD15s) was associated with CD117 positivity in the majority of AML cases (64 vs. 85%, P = 0.043). Radioimmunotherapeutic strategies targeting CD66 antigens should consider the heterogeneous expression pattern of CD66 molecules in acute leukemias especially in AML where expression is correlated with mature granulomonocytic cells but not with CD34 and CD117 positive progenitor cells.
Ratei
Richard
R
HELIOS Klinikum Berlin, Charité-University Medical School Berlin, Robert-Roessle-Clinic, Campus-Buch, Schwanebecker Chaussee 50, 13125, Berlin, Germany. richard.ratei@helios-kliniken.de.
Karawajew
Leonid
L
HELIOS Klinikum Berlin, Charité-University Medical School Berlin, Robert-Roessle-Clinic, Campus-Buch, Schwanebecker Chaussee 50, 13125, Berlin, Germany.
Schabath
Richard
R
HELIOS Klinikum Berlin, Charité-University Medical School Berlin, Robert-Roessle-Clinic, Campus-Buch, Schwanebecker Chaussee 50, 13125, Berlin, Germany.
Ehrfeldt
Angelika
A
Institute for Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany.
Grunert
Fritz
F
Genovac, Freiburg, Germany.
Ludwig
Wolf-Dieter
WD
HELIOS Klinikum Berlin, Charité-University Medical School Berlin, Robert-Roessle-Clinic, Campus-Buch, Schwanebecker Chaussee 50, 13125, Berlin, Germany.
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D058851:GPI-Linked Proteins; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000145:classification / Q000378:metabolism*; D008875:Middle Aged; D009844:Oligosaccharides / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
D015703:Antigens, CD; C069847:CD66 antigens; C504117:CEACAM6 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen