[Effects of diazoxide on Fas/FasL protein expressions in rat myocardium suffered from long-term hypothermic preservation].
The purpose of this study was to investigate the effect of a mitochondrial ATP-sensitive potassium channel (mitoK(ATP)) opener, diazoxide (DE), on Fas/FasL protein expressions in rat heart suffered from long-term hypothermic preservation. The Langendorff isolated rat heart model was used. The hearts were stored in 4 °C Celsior solution with or without (control) DE for 8 h followed by 60 min of reperfusion. The recovery of rate-pressure product (RPP) was observed. Apoptotic cardiomyocytes were detected by TdT-mediated dUTP nick end labeling (TUNEL) technique. The expressions of Fas/FasL proteins were also analyzed by immunohistochemical method. The results showed that compared with the control group, DE (30 mmol/L) increased the recovery of RPP during reperfusion, reduced the percentage of apoptotic cells and the expressions of Fas and FasL proteins in rat hearts suffered from 8 h of hypothermic preservation. The above effects of DE were attenuated by a mitoK(ATP) channel inhibitor 5-hydroxydecanoate (5-HD). These results indicate that DE could alleviate rat myocardial injury induced by ischemia-reperfusion through reducing the expressions of Fas and FasL proteins via opening of mitoK(ATP)channel.
Fan
Ying
Y
Department of Physiology, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Zheng
Ming-Zhi
MZ
Guo
Wei
W
Jiang
Jian-Ping
JP
Zhu
Li
L
Shen
Yue-Liang
YL
Chen
Ying-Ying
YY
D000818:Animals; D017209:Apoptosis; D015925:Cryopreservation; D003652:Decanoic Acids / Q000494:pharmacology; D003981:Diazoxide / Q000494:pharmacology*; D053222:Fas Ligand Protein / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D006880:Hydroxy Acids / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects; D026902:Potassium Channel Blockers / Q000494:pharmacology; D015221:Potassium Channels; D051381:Rats; D019014:fas Receptor / Q000378:metabolism*
D003652:Decanoic Acids; D053222:Fas Ligand Protein; C491898:Fas protein, rat; C505662:Faslg protein, rat; D006880:Hydroxy Acids; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D019014:fas Receptor; C117780:mitochondrial K(ATP) channel; C052853:5-hydroxydecanoic acid; D003981:Diazoxide