title: Biotinylated nanodiamond: simple and efficient functionalization of detonation diamond.
abstract: We have developed a simple and efficient method for the covalent functionalization of detonation nanodiamond. After homogenization of the surface by borane reduction, the surface was modified with (3-aminopropyl)trimethoxysilane. Subsequent grafting of biotin yielded covalently biotinylated nanodiamond, which was characterized by FTIR spectroscopy, X-ray powder diffractometry, thermogravimetry, and elemental analysis. The activity was tested with horseradish peroxidase-labeled streptavidin. The surface loading of biotin was found to be 1.45 mmol g-1. The new material opens the way to covalently bonded diamond bioconjugates for labeling, drug delivery, and other applications.
authors.affiliation: Otto-Diels-Institut für Organische Chemie, Christian-Albrechts-Universität zu Kiel, Otto-Hahn-Platz 3, Kiel, Germany. akrueger@oc.uni-kiel.de
authors.forename: Anke
authors.initials: A
authors.lastname: Krueger
authors.forename: Jochen
authors.initials: J
authors.lastname: Stegk
authors.forename: Yuejiang
authors.initials: Y
authors.lastname: Liang
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Gerald
authors.initials: G
authors.lastname: Jarre
mesh_terms: D019921:Biotinylation; D006112:Gravitation; D006735:Horseradish Peroxidase / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
chemical_list: D019809:Streptavidin; D006735:Horseradish Peroxidase