Renal regional proteomes in young Dahl salt-sensitive rats.
We performed an extensive proteomic analysis of the Dahl model of salt-sensitive hypertension. The consomic SS-13(BN) rat, genetically similar to the Dahl salt-sensitive rat, while exhibiting a significant amelioration of salt-induced hypertension, was used as a control. Proteomic analysis, using differential in-gel electrophoresis and mass spectrometry techniques, was performed in the renal cortex and the renal medulla of 6-week-old SS and SS-13(BN) rats before significant differences in blood pressure were developed between the 2 strains of rat. Several dozen proteins were identified as differentially expressed between SS and SS-13(BN) rats fed the 0.4% NaCl diet or switched to the 4% NaCl diet for 3 days (n=4). The identified proteins were involved in cellular functions or structures including signal transduction, energy metabolism, and the cytoskeleton. The proteomic analysis and subsequent Western blotting indicated that heterogeneous nuclear ribonucleoprotein K in the renal medulla was upregulated by the 4% NaCl diet in SS-13(BN) rats but downregulated in SS rats. The level of angiotensinogen mRNA in the renal medulla was regulated in an opposite manner. Silencing of heterogeneous nuclear ribonucleoprotein K resulted in an upregulation of angiotensinogen in cultured human kidney cells. In summary, we identified significant differences in kidney regional proteomic profiles between SS and SS-13(BN) rats and demonstrated a potential role of heterogeneous nuclear ribonucleoprotein K in the regulation of angiotensinogen expression in the renal medulla.
Tian
Zhongmin
Z
Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Greene
Andrew S
AS
Usa
Kristie
K
Matus
Isaac R
IR
Bauwens
Jesse
J
Pietrusz
Jennifer L
JL
Cowley
Allen W
AW
Liang
Mingyu
M
D000367:Age Factors; D000808:Angiotensinogen / Q000235:genetics; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D004586:Electrophoresis; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000502:physiology; D006977:Hypertension, Renal / Q000139:chemically induced* / Q000503:physiopathology*; D007668:Kidney / Q000166:cytology / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D040901:Proteomics; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020303:Rats, Inbred Dahl; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*
D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D012333:RNA, Messenger; D017673:Sodium Chloride, Dietary; D000808:Angiotensinogen