High-affinity sulfonated materials with transition metal counterions for enhanced protein separation in dual-layer hollow fiber membrane chromatography.
High-affinity membrane materials have been successfully synthesized through a combination of the polymer sulfonation reaction with transition metal counterion exchange treatment. This type of promising materials were embodied for the first time with the aid of dual-layer hollow fiber technology for protein separation. Three types of immobilized metal affinity membranes (Cu(2+), Ni(2+) and Zn(2+) forms) were developed in this work and they all exhibited enhanced protein separation performance compared to the as-spun hollow fiber in H(+) form due to the strong affinity between transition metal counterions and target protein molecules. Ultimately, the high-purity target protein (>99%, w/w) could be achieved via the membrane in Cu(2+) form.
Li
Yi
Y
Department of Chemical and Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Singapore.
Chung
Tai-Shung
TS
Chan
Sui Yung
SY
D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D006454:Hemoglobins / Q000302:isolation & purification*; D008567:Membranes, Artificial; D009532:Nickel / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D013450:Sulfones / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
D006454:Hemoglobins; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; D012710:Serum Albumin, Bovine; D003300:Copper; D009532:Nickel; D015032:Zinc