Effects of human bone marrow stromal cell line (HFCL) on the proliferation, differentiation and apoptosis of acute myeloid leukemia cell lines U937, HL-60 and HL-60/VCR.
This study investigated the effects of human bone marrow fibroblastoid stromal cell line (HFCL) on the proliferation, differentiation and chemosensitivity of acute myeloid leukemia cells (AML) in vitro coculture. By setting up coculture system of sensitive U937, HL-60 cell line and multidrug-resistant (MDR) HL-60/VCR cells in direct contact with human bone marrow fibroblastoid stromal cell line HFCL, or separated by transwell, the proliferation of AML cells cocultured with HFCL cells was inhibited, compared with AML cells alone. And NBT positive cells increased slightly. The percentage of G1 phase cells of AML cells cocultured with HFCL cells was higher than that without HFCL cells, and that of S phase cells was lower. The expression of CD11b and CD14 increased. Meanwhile HL-60 and HL-60/VCR cells treated by TPT were observed to have apoptosis characteristic morphological changes. The proportion of G0/G1 HL-60 and HL-60/VCR cells treated with TPT increased and the sub-G1 increased. The percentage of Annexin V-positive cells and apoptotic cells increased with expression of activated Caspase-3 and the reduced expression of Bcl-2. But when they were cocultured with HFCL cells, the percentage of Annexin V-positive cells and apoptotic cells decreased and sub-G1 reduced. After indirect contact with HFCL cells the expression of activated Caspase-3 decreased and the expression of Bcl-2 increased. After direct contact with HFCL cells for 96 h, the expression levels of 582 genes in HL-60 cells were up-regulated, and 1,323 genes were down-regulated at least twofold by Affymetrix GeneChip Human Genome U133 set A. The expression change in some genes, such as HL14, was confirmed by RT-PCR and northern blot. In a word, HFCL cells could inhibit the proliferation, induce the monocytic differentiation of U937, HL-60 and HL-60/VCR cells, and prevent TPT-induced apoptosis in HL-60 and HL-60/VCR cells via modulation of Bcl-2 and active Caspase-3. Many genes might take part in the influence of HFCL cells on AML cells, which may give important insights into the interaction of bone marrow stromal cells and leukemic cells.
Liang
Rong
R
Department of Hematology, Xijing Hospital, Fourth Military Medical University, No. 17, West Changle Road, 710032, Xi'an, People's Republic of China. liangjh@fmmu.edu.cn.
Huang
Gao-Sheng
GS
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an, People's Republic of China.
Wang
Zhe
Z
Department of Pathology, Xijing Hospital, Fourth Military Medical University, Xi'an, People's Republic of China.
Chen
Xie-Qun
XQ
Department of Hematology, Xijing Hospital, Fourth Military Medical University, No. 17, West Changle Road, 710032, Xi'an, People's Republic of China.
Bai
Qin-Xian
QX
Department of Hematology, Xijing Hospital, Fourth Military Medical University, No. 17, West Changle Road, 710032, Xi'an, People's Republic of China.
Zhang
Yong-Qing
YQ
Department of Hematology, Xijing Hospital, Fourth Military Medical University, No. 17, West Changle Road, 710032, Xi'an, People's Republic of China.
Dong
Bao-Xia
BX
Department of Hematology, Xijing Hospital, Fourth Military Medical University, No. 17, West Changle Road, 710032, Xi'an, People's Republic of China.
Wang
Wen-Qing
WQ
Department of Hematology, Xijing Hospital, Fourth Military Medical University, No. 17, West Changle Road, 710032, Xi'an, People's Republic of China.
D017209:Apoptosis; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D017154:Stromal Cells; D015854:Up-Regulation