Epitope mapping of 2E2-D3, a monoclonal antibody directed against human TDP-43.
TDP-43 is now known to be a major component of ubiquitin-positive, tau-negative inclusions in frontotemporal lobar degeneration with ubiquitin-positive inclusions and sporadic amyotrophic lateral sclerosis. In this study, we mapped the epitope for the monoclonal anti-TDP-43 antibody 2E2-D3. Our mapping and peptide competition experiments showed that the antibody reacted with human TDP-43, but not mouse or rat TDP-43, and recognized amino acids 205-222 of human TDP-43, corresponding to a part of the second RNA recognition motif. These findings suggest that 2E2-D3 is a useful antibody for the characterization of mouse lines transgenic for human TDP-43.
Zhang
Hai-Xin
HX
Department of Neuropathology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Japan.
Tanji
Kunikazu
K
Mori
Fumiaki
F
Wakabayashi
Koichi
K
D000690:Amyotrophic Lateral Sclerosis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000276:immunology*; D003704:Dementia / Q000276:immunology*; D018604:Epitope Mapping; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D016296:Mutagenesis; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins