Aldose-reductase- and protein-glycation-inhibitory principles from the whole plant of Duchesnea chrysantha.
Ellagic acid (1), 3,3'-di-O-methylellagic acid (2), 3,3',4-tri-O-methylellagic acid (3), isovitexin (4), kaempferol 3-O-beta-D-glucuronide methyl ester (5), quercetin 3-O-alpha-L-arabinopyranosyl-(1-->6)-beta-D-galactopyranoside (6), ursolic acid, pomolic acid, tormentic acid, euscaphic acid, euscaphic acid 28-O-beta-D-glucopyranoside, and maslinic acid were isolated from the AcOEt- and BuOH-soluble MeOH extract of Duchesnea chrysantha (whole plant). The isolates were subjected to in vitro bioassays to evaluate their inhibitory activity on rat-lens aldose reductase (RLAR) and formation of advanced glycation end products (AGEs). The ellagic acids and flavonoids, compounds 1-6, exhibited moderate inhibitory effects on RLAR. However, compounds 1 and 4-6 showed excellent inhibitory activities towards the formation of AGEs. This is the first report that 4 and 6 exhibit inhibitory activity towards AR and AGEs formation.
Kim
Jong Min
JM
Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Daejeon, 305-812, Korea.
Jang
Dae Sik
DS
Lee
Yun Mi
YM
Yoo
Jeong Lim
JL
Kim
Young Sook
YS
Kim
Joo-Hwan
JH
Kim
Jin Sook
JS
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D000818:Animals; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D004610:Ellagic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000201:enzymology; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae / Q000737:chemistry*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000097245:Ursolic Acid
D000449:Aldehyde Reductase; D047310:Apigenin; D004610:Ellagic Acid; D017127:Glycation End Products, Advanced; D006027:Glycosides; D009828:Oleanolic Acid; D011794:Quercetin; D014315:Triterpenes; D000097245:Ursolic Acid; C050401:3,3'-di-O-methylellagic acid; C007695:euscaphic acid; C115535:pomolic acid; C412811:maslinic acid; C049772:isovitexin