title: Continuous PGE2 leads to net bone loss while intermittent PGE2 leads to net bone gain in lumbar vertebral bodies of adult female rats.
abstract: The present study examined the effects of continuous and intermittent PGE2 administration on the cancellous and cortical bone of lumbar vertebral bodies (LVB) in female rats. Six-month-old Sprague-Dawley female rats were divided into 6 groups with 2 control groups and 1 or 3 mg PGE2/kg given either continuously or intermittently for 21 days. Histomorphometric analyses were performed on the cancellous and cortical bone of the fourth and fifth LVBs. Continuous PGE2 exposure led to bone catabolism while intermittent administration led to bone anabolism. Both routes of administration stimulated bone remodeling, but the continuous PGE2 stimulated more than the intermittent route to expose more basic multicellular units (BMUs) to the negative bone balance. The continuous PGE2 caused cancellous bone loss by stimulating bone resorption greater than formation (i.e., negative bone balance) and shortening the formation period. It caused more cortical bone loss than gain, the magnitude of the negative endocortical bone balance and increased intracortical porosity bone loss was greater than for periosteal bone gain. The anabolic effects of intermittent PGE2 resulted from cancellous bone gain by positive bone balance from stimulated bone formation and shortened resorption period; while cortical bone gain occurred from endocortical bone gain exceeding the decrease in periosteal bone and increased intracortical bone loss. Lastly, a scheme to take advantage of the marked PGE2 stimulation of lumbar periosteal apposition in strengthening bone by converting it to an anabolic agent was proposed.
authors.affiliation: Division of Radiobiology, Department of Radiology, University of Utah School of Medicine, 729 Arapeen Dr., Suite 2338, Salt Lake City, UT, 84108-1218, USA.
authors.forename: X Y
authors.initials: XY
authors.lastname: Tian
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Zhao
authors.forename: R B
authors.initials: RB
authors.lastname: Setterberg
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Zeng
authors.forename: S J
authors.initials: SJ
authors.lastname: Iturria
authors.forename: Y F
authors.initials: YF
authors.lastname: Ma
authors.forename: W S S
authors.initials: WS
authors.lastname: Jee
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000187:drug effects*; D001862:Bone Resorption / Q000473:pathology; D015232:Dinoprostone / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008159:Lumbar Vertebrae / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015232:Dinoprostone