title: Simple and rapid determination of serum carbapenem concentrations by high-performance liquid chromatography.
abstract: Although several high-performance liquid chromatography (HPLC) methods for the determination of serum concentrations of carbapenems have been reported, they are complicated and involve column switching. We established a simple and rapid HPLC method for the determination of serum concentrations of carbapenems, and this method is suitable for routine use in the clinical field. With our HPLC method, the serum concentrations of five commercially available carbapenems could be determined by changing only the methanol/phosphate-buffer ratio in the mobile phase. Serum levels of imipenem, panipenem, and meropenem in mice could be monitored when these carbapenems (20 mg/kg) were administered subcutaneously with cilastatin (20 mg/kg). These results suggest that our simple and rapid HPLC method for the determination of the serum concentrations of carbapenems is useful for pharmacokinetic/pharmacodynamic (PK/PD)-based determination of carbapenem dosage regimens.
authors.affiliation: Department of Practical Pharmacy, Kyoritsu University of Pharmacy, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kurihara
authors.forename: Junko
authors.initials: J
authors.lastname: Kizu
authors.forename: Seiji
authors.initials: S
authors.lastname: Hori
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood; D015780:Carbapenems / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D015378:Imipenem / Q000097:blood; D008297:Male; D000077731:Meropenem; D051379:Mice; D013845:Thienamycins / Q000097:blood
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D013845:Thienamycins; D015378:Imipenem; D000077731:Meropenem; C039536:panipenem