title: The effects of Ankrd2 alteration indicate its involvement in cell cycle regulation during muscle differentiation.
abstract: Ankrd2 is a member of the Muscle Ankyrin Repeat Protein family (MARPs), consisting of sarcomere-associated proteins that can also localize in the nucleus. There are indications that MARPs might function as shuttle proteins between the cytoplasm and nucleus, likely sending information to the nucleus concerning the changes in the structure or function of the contractile machinery. Even though recent findings suggest that the MARP gene family is not essential for the basal functioning of skeletal muscle, its influence on the gene expression program of skeletal muscle cells was highlighted. To investigate this regulatory role we produced and examined both morphological and functional features of myocytes stable overexpressing or silencing the Ankrd2 protein. The transcriptional profiles of the myocytes revealed that the molecular pathways perturbed by changes in Ankrd2 protein level are congruent with the morpho-physiological and biochemical data obtained in Ankrd2-modified myoblasts induced to differentiate. Our results suggest that Ankrd2 gives an important contribution to the coordination of proliferation and apoptosis during myogenic differentiation in vitro, mainly through the p53 network.
authors.affiliation: Department of Biology and CRIBI Biotechnology Centre, University of Padova, Via Ugo Bassi 58/b, 35131 Padova, Italy.
authors.forename: Camilla
authors.initials: C
authors.lastname: Bean
authors.forename: Nicola
authors.initials: N
authors.lastname: Facchinello
authors.forename: Georgine
authors.initials: G
authors.lastname: Faulkner
authors.forename: Gerolamo
authors.initials: G
authors.lastname: Lanfranchi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000502:physiology*; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D015870:Gene Expression / Q000502:physiology; D020869:Gene Expression Profiling; D051379:Mice; D032342:Muscle Cells / Q000166:cytology / Q000378:metabolism; D009124:Muscle Proteins / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology*; D032446:Myoblasts / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D014162:Transfection
chemical_list: C485877:Ankrd2 protein, mouse; D009124:Muscle Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D053148:Caspase 3