Cyanine dyes for the detection of double stranded DNA.
Twenty three novel cyanine dyes have been applied as fluorescent stains for the detection of nucleic acids in agarose gel electrophoresis. Significant fluorescence enhancement of these dyes in the presence of double stranded DNA was observed. Five dyes offered superior sensitivity in the detection and quantification of DNA, over Ethidium Bromide, the most commonly used nucleic acid stain.
Hilal
H
H
School of Materials, Faculty of Engineering and Physical Sciences, University of Manchester, M60 1QD, United Kingdom. haithem_hilal@hotmail.com
Taylor
J A
JA
D002232:Carbocyanines / Q000032:analysis* / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D004926:Escherichia coli; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D002232:Carbocyanines; D004247:DNA