IFP35 is involved in the antiviral function of interferon by association with the viral tas transactivator of bovine foamy virus.
Interferon-induced proteins (IFPs) exert multiple functions corresponding to diverse interferon signals. However, the intracellular functions of many IFPs are not fully characterized. Here, we report that IFP35, a member of the IFP family with a molecular mass of 35 kDa, can interact with the bovine Tas (BTas) regulatory protein of bovine foamy virus (BFV). The interaction involves NID2 (IFP35/Nmi homology domain) of IFP35 and the central domain of BTas. The overexpression of IFP35 disturbs the ability of BTas to activate viral-gene transcription and inhibits viral replication. The depletion of endogenous IFP35 by interfering RNA can promote the activation of BFV, suggesting an inhibitory function of IFP35 in viral-gene expression. In addition, IFP35 can interact with the homologous regulatory protein of prototype FV and arrest viral replication and repress viral transcription. Our study suggests that IFP35 may represent a novel pathway of interferon-mediated antiviral activity in host organisms that plays a role in the maintenance of FV latency.
Tan
Juan
J
Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education), College of Life Sciences, Nankai University, Tianjin 300071, China. gengyq@nankai.edu.cn
Qiao
Wentao
W
Wang
Jian
J
Xu
Fengwen
F
Li
Yue
Y
Zhou
Jun
J
Chen
Qimin
Q
Geng
Yunqi
Y
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007372:Interferons; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000821:virology; D034741:RNA, Small Interfering / Q000494:pharmacology; D016092:Spumavirus / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism*; D017735:Virus Latency
C085378:IFI35 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D015534:Trans-Activators; D007372:Interferons