Molecular cloning and beta-naphthoflavone-induced expression of a cytochrome P450 1A (CYP1A) gene from an anadromous river pufferfish, Takifugu obscurus.
In recent years, there has been a decline in the wild populations of river pufferfish, Takifugu obscurus. Besides overexploitation for commercial purposes, environmental pollution is believed to have contributed to its decline. However, almost no information exists about genes involved in metabolism of xenobiotics by this species. Nevertheless, there is interest in fugu fishes, since they possess the smallest genome among vertebrates. We cloned and characterized the full-length cDNA sequence of a cytochrome P450 1A (CYP1A) gene from T. obscurus. Phylogenic relationship of T. obscurus CYP1A was also compared to other fish species. The tissue distribution and time-dependant induction of CYP1A mRNA were studied by real-time PCR in T. obscurus exposed to an aryl hydrocarbon receptor (Ahr) agonist, beta-naphthoflavone (BNF). The greatest basal expression in livers of control as well as BNF-treated individuals. However, brain, gill, gonad, intestine, and kidney also expressed CYP1A. Muscles expressed the least CYP1A. The results of the time-course study revealed induction in brain and gills after 6h and at 12h in most tissues. Except for gills, all other organs retained induced expression of CYP1A mRNA up to 96h.
Kim
Jin-Hyoung
JH
Department of Marine Science, College of Natural Sciences, Inha University, Incheon 402-751, South Korea.
Raisuddin
Sheikh
S
Ki
Jang-Seu
JS
Lee
Jae-Seong
JS
Han
Kyung-Nam
KN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019363:Cytochrome P-450 CYP1A1 / Q000737:chemistry / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008969:Molecular Sequence Data; D010802:Phylogeny; D023741:Takifugu / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D019324:beta-Naphthoflavone / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1