title: Late phase TNF-alpha depression in natural orifice translumenal endoscopic surgery (NOTES) peritoneoscopy.
abstract: BACKGROUND
  Natural orifice translumenal endoscopic surgery (NOTES) allows access to the peritoneal cavity without skin incisions. Contamination of the peritoneal cavity by enteric contents may render NOTES more physiologically and immunologically invasive than previously thought. Measurement of interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) is a validated method to quantify surgical stress. The physiologic and immunologic impact of NOTES is unknown.
  
  
  METHODS
  A total of 37 swine underwent abdominal exploration via transgastric NOTES peritoneoscopy, laparoscopy (LX), laparotomy (OPEN), or sham surgery (CONTROL) and were allowed to survive. TNF-alpha, IL-1beta, and IL-6 plasma levels were determined at the start and completion of surgery, and at 1 hour, 2 days, and 14 days postoperatively.
  
  
  RESULTS
  At surgical completion, OPEN animals demonstrated higher TNF-alpha levels than all groups. TNF-alpha levels were similar for all groups at 1 hour and 2 days. NOTES animals had significantly reduced plasma levels of TNF-alpha than all other groups on postoperative days 7 and 14. Controlling for baseline cytokine variability, analysis was repeated using normalized data, which confirmed significantly reduced TNF-alpha levels for NOTES compared with all groups at 14 days. Subset analysis excluding LX and OPEN complications revealed lower NOTES TNF-alpha levels at 7 and 14 days compared with all groups. IL-1beta and IL-6 levels were undetectable in 66.8% and 70.5% of samples, respectively, without significant trends.
  
  
  CONCLUSIONS
  Diagnostic NOTES peritoneoscopy demonstrated similar levels of systemic proinflammatory cytokine TNF-alpha compared with diagnostic laparoscopy and exploratory laparotomy in the immediate postoperative period despite gross intraperitoneal contamination. None of the surgical groups, however, produced a measurable, consistent trend in IL-1beta or IL-6. Consistently reduced levels of TNF-alpha in NOTES animals in the late postoperative period indicates an immunomodulatory effect of the NOTES surgical technique not present in laparoscopy or laparotomy.
authors.affiliation: Case Advanced Surgical Endoscopy Team (CASE-T) Laboratory, Departments of Surgery and Gastroenterology, Case Medical Center, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: Michael F
authors.initials: MF
authors.lastname: McGee
authors.forename: Steve J
authors.initials: SJ
authors.lastname: Schomisch
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Marks
authors.forename: Conor P
authors.initials: CP
authors.lastname: Delaney
authors.forename: Judy
authors.initials: J
authors.lastname: Jin
authors.forename: Christina
authors.initials: C
authors.lastname: Williams
authors.forename: Amitabh
authors.initials: A
authors.lastname: Chak
authors.forename: David T
authors.initials: DT
authors.lastname: Matteson
authors.forename: Jamie
authors.initials: J
authors.lastname: Andrews
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Ponsky
mesh_terms: D000818:Animals; D004724:Endoscopy; D005260:Female; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D010535:Laparoscopy; D007813:Laparotomy; D009055:Mouth; D010529:Peritoneal Cavity / Q000601:surgery*; D010538:Peritonitis / Q000276:immunology; D011183:Postoperative Complications / Q000276:immunology*; D034421:Sus scrofa; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha