[New fluorescent staining method to distinguish normal and malignant rectum tissues using terbium (III)-ciprofloxacin complex].
A new fluorescent staining method to distinguish normal and malignant rectum tissues using terbium(III)-Ciprofloxacin complex was investigated. The terbium (III) complex exhibits strong fluorescence and the staining process with the complex is very fast. It needs 3-5 minutes. The results showed that the fluorescence microscope images were similar to the images stained using traditional hematoxylin and eosin (H&E) methodology. The fluorescence spectra and FTIR spectra of normal and malignant rectum tissues were collected and the detection criterions for the malignant rectum tissues were the same as the staining results. In summary, the fluorescence staining may be developed as a simple and convenient method.
Meng
Tao
T
State Key Laboratory of Polymer Physics and Chemistry, Joint Laboratory of Polymer Science and Materials, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Xu
Duan-fu
DF
Zhao
Ying
Y
Wang
Du-jin
DJ
Xu
Yi-zhuang
YZ
Qi
Jian
J
Wu
Jin-guang
JG
D002939:Ciprofloxacin / Q000737:chemistry*; D005453:Fluorescence; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D012004:Rectal Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D012007:Rectum / Q000737:chemistry / Q000166:cytology / Q000473:pathology*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D013725:Terbium / Q000737:chemistry*
D013725:Terbium; D002939:Ciprofloxacin