[Expression of Omi/HtrA2 UP-regulated in the spermatozoa of chronic bacterial prostatitis patients].
OBJECTIVE
To determine the expression of Omi/HtrA2 in the spermatozoa of chronic bacterial prostatitis patients and explore the potential mechanism of chronic prostatitis inducing male infertility.


METHODS
Forty-one patients diagnosed as suffering from chronic prostatitis were included, and so were 12 healthy normal men as controls. Spermatozoa in the semen were purified by Percoll gradient technique to separate the seminal plasma and other round cells. Sperm concentration, motility, morphology, pro-inflammatory cytokines, Omi/HtrA2 mRNA and protein levels in the spermatozoa of the patients were measured.


RESULTS
Significantly increased levels of pro-inflammatory cytokines were observed in the seminal plasma of the prostatitis patients, and so were Omi/HtrA2 mRNA and protein levels as compared with the normal men.


CONCLUSIONS
Chronic prostatitis patients presented important alterations in semen quality parameters and up-regulated expression of Omi/HtrA2 mRNA and proteins in the spermatozoa.
Hu
Xiao-Yong
XY
Department of Urology, 6th People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China. jd6hyong@yahoo.com
Xu
Yue-Min
YM
Qiao
Yong
Y
Liu
Zhang-Shun
ZS
Song
Lu-Jie
LJ
Li
Chao
C
D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D015870:Gene Expression; D000074923:High-Temperature Requirement A Serine Peptidase 2; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D011472:Prostatitis / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D053583:Interleukin-1beta; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D012697:Serine Endopeptidases; C000615914:HTRA2 protein, human; D000074923:High-Temperature Requirement A Serine Peptidase 2