title: Amperometric immunosensor for diagnosis of BLV infection.
abstract: A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.
authors.affiliation: Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania.
authors.forename: Bogumila
authors.initials: B
authors.lastname: Kurtinaitiene
authors.forename: Dovile
authors.initials: D
authors.lastname: Ambrozaite
authors.forename: Valdas
authors.initials: V
authors.lastname: Laurinavicius
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis / Q000821:virology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000032:analysis*
chemical_list: D014759:Viral Envelope Proteins