Failure of ES-62 to inhibit T-helper type 1 responses to other filarial nematode antigens.
ES-62 is a secreted protein of filarial nematodes that possesses multiple immunomodulatory activities. A full characterization of these activities awaits elucidation but to date it has been shown that ES-62 can inhibit pro-inflammatory/Th1 immune responses and in some studies, it has been found to actively support Th2 development. As an active filarial nematode infection is associated with a Th2-like immunological phenotype, this study investigated whether ES-62 was likely to be responsible for, or at least contribute to, this phenotype. Specifically, we determined ES-62's effect on the immune response to two other filarial nematode antigens, chosen for their ability to promote Th1 responses. The two antigens were recombinant Onchocerca volvulus-Fatty acid And Retinol-binding-1 (rOv-FAR-1) and recombinant Onchocerca volvulus-Activation associated Secreted Protein-1 (Ov-ASP-1). Overall the results show that in spite of its previously characterized immunomodulatory properties, ES-62 was unable to modulate/reverse the Th1 immune responses induced by the two Onchocerca antigens. Therefore, in this study no support is provided for the idea that ES-62 might be a major player in facilitating the overall immunological phenotype in filariasis and reasons for this somewhat surprising outcome are discussed.
Al-Riyami
L
L
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow G4 0NR, UK.
Egan
C A
CA
Bradley
J E
JE
Lustigman
S
S
Harnett
W
W
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D007155:Immunologic Factors / Q000276:immunology*; D007371:Interferon-gamma / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D017181:Onchocerca volvulus / Q000276:immunology*; D009855:Onchocerciasis / Q000276:immunology* / Q000469:parasitology; D011994:Recombinant Proteins / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*
D000947:Antigens, Helminth; C435884:ES-62 protein, Acanthocheilonema viteae; D015801:Helminth Proteins; D007074:Immunoglobulin G; D007155:Immunologic Factors; D011994:Recombinant Proteins; D007371:Interferon-gamma