title: Rapid determination of carbamate pesticides in food using dual counter-current chromatography directly interfaced with mass spectrometry.
abstract: Dual counter-current chromatography (dual CCC)-tandem mass spectrometry (MS/MS) was successfully performed with a newly designed spiral column for dual CCC. The small column capacity required for directly coupling with electrospray MS/MS was accomplished by forming a rectangular spiral groove on a plastic disk and sealing it with a PTFE sheet. This novel dual CCC-MS/MS technique was successfully applied for the rapid determination of methomyl, fenobucarb and carbaryl pesticides in food. A two-phase solvent system of n-hexane-acetonitrile-0.1% formic acid (45:45:10) was suitable for both good dual CCC separation and sufficient ionization of pesticides. Recoveries of these three pesticides from mandarin orange and spinach samples fortified at 0.05 mg/kg were in the range of 93-107% with relative standard deviations of 2.4-3.8%.
authors.affiliation: Aichi Prefectural Institute of Public Health, Tsuji-machi, Kita-ku, Nagoya 462-8576, Japan. yuuko_1_ito@pref.aichi.lg.jp
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ito
authors.forename: Tomomi
authors.initials: T
authors.lastname: Goto
authors.forename: Sadaji
authors.initials: S
authors.lastname: Yamada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Ohno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Hisao
authors.initials: H
authors.lastname: Oka
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002219:Carbamates / Q000032:analysis*; D012721:Carbaryl / Q000032:analysis; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D008724:Methomyl / Q000032:analysis; D010575:Pesticides / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014675:Vegetables / Q000737:chemistry
chemical_list: D002219:Carbamates; D010575:Pesticides; D010938:Plant Oils; D008724:Methomyl; C019276:BPMC; D012721:Carbaryl