Electrostatic interactions between a protein and oppositely charged micelles.
Micellar solutions made of a fully fluorinated surfactant, LiPFN, form water-soluble complexes with lysozyme in a wide concentration range. Such complexes are stabilized by electrostatic and, very presumably, double-layer interactions. The mixtures were investigated by combining electrophoretic mobility, DLS, and dielectric relaxation methods. The former gives information on the surface charge density of protein-micelle complexes and indicates that the resulting adducts retain a negative charge (i.e., charge neutralization is incomplete). The double-layer thickness of proteins, micelles, and protein-micelle complexes is also connected to the dielectric relaxation frequency. Changes in particle size (inferred by DLS), charge density, and double-layer thickness are closely interrelated to each other. A model was developed to quantify such properties.
Andreozzi
Patrizia
P
Dipartimento di Chimica, SOFT-INFM-CNR Research Center, La Sapienza University, Rome, Italy.
Bonincontro
Adalberto
A
La Mesa
Camillo
C
D005466:Fluorocarbons / Q000737:chemistry*; D008823:Micelles; D009113:Muramidase / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D005466:Fluorocarbons; D008823:Micelles; D013501:Surface-Active Agents; D014867:Water; D009113:Muramidase