Use of the gyrB gene to discriminate among species of the Burkholderia cepacia complex.
Bacteria of the Burkholderia cepacia complex (Bcc) are opportunistic pathogens that can cause serious infections in lungs of cystic fibrosis patients. The Bcc comprises at least nine species that have been discriminated by a polyphasic taxonomic approach. In this study, we focused on the gyrB gene, universally distributed among bacteria, as a new target gene to discriminate among the Bcc species. New PCR primers were designed to amplify a gyrB DNA fragment of about 1900 bp from 76 strains representative of all Bcc species. Nucleotide sequences of PCR products were determined and showed more than 400 polymorphic sites with high sequence similarity values from most isolates of the same species. Phylogenetic tree analysis revealed that most of the 76 gyrB sequences grouped, forming clusters, each corresponding to a given Bcc species.
Tabacchioni
Silvia
S
ENEA - CRE - CASACCIA -Department of Biotechnologies, Agroindustry, Protection of Health - Plant genetics and Genomics Section, S. Maria di Galeria, Rome, Italy.
Ferri
Lorenzo
L
Manno
Graziana
G
Mentasti
Massimo
M
Cocchi
Priscilla
P
Campana
Silvia
S
Ravenni
Novella
N
Taccetti
Giovanni
G
Dalmastri
Claudia
C
Chiarini
Luigi
L
Bevivino
Annamaria
A
Fani
Renato
R
D001426:Bacterial Proteins / Q000235:genetics*; D019121:Burkholderia Infections / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D027081:DNA Gyrase / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D027081:DNA Gyrase