Development of a gas chromatography-time-of-flight mass spectrometry method for the determination of buagafuran, a promising antianxiety drug in dog blood.
A sensitive gas chromatography-time-of-flight mass spectrometry (GC-ToFMS) method has been developed and validated for the determination of buagafuran, a new anxiolytic drug derived from alpha-agarofuran. Buagafuran and internal standard (buagafuran-d(4)) were isolated from plasma by liquid-liquid extraction. The separation was achieved on HP-1 capillary column (25 m x 0.2 mm x 0.11 microm). Buagafuran (m/z 262.22) and buagafuran-d(4) (m/z 266.25) were eluted at 7.6 min and no endogenous materials interfered with the measurement. The calibration curves of buagafuran were linear over the range of 2.5-160 ng/ml in dog plasma. The lower limit of quantification (LLoQ) was 2.5 ng/ml in plasma. The within-day and between-day precisions were less than 15%. The method was used to determine the plasma concentration-time profile of buagafuran after oral doses of 8, 16, 32 mg/kg in dogs.
Hu
Jinping
J
Institute of Materia Medica, Chinese Academy of Medical Sciences & Perking Union Medical College, Beijing, PR China.
Chen
Hui
H
Li
Yan
Y
D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002138:Calibration; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D005260:Female; D005615:Freezing; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation; D008297:Male; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D013997:Time Factors
D014151:Anti-Anxiety Agents; D012717:Sesquiterpenes; C500825:alpha-agarofuran; C530322:buagafuran