title: Japanese encephalitis virus envelope protein mitigates TNF-alpha mRNA expression in RAW264.7 cells.
abstract: Japanese encephalitis virus (JEV) is known as an important mosquito-borne human pathogen that causes Japanese encephalitis and may lead to lethal effect. Since monocyte has been demonstrated to play transmissible role for JEV, rare study is reported to clarify the effect of JEV envelope (JEVE) protein on monocyte. This study intends to investigate the effects of JEVE protein inside monocyte. Notably, significant decreased expression of tumour necrosis factor (TNF)-alpha mRNA in RAW264.7 cells transfected with pEGFP-JEVE was observed as compared to those cells transfected with pEGFP. Increased p21(Waf1/Cip1) protein was observed in both pEGFP and pEGFP-JEVE transfected RAW264.7 cells. However, increased p53 protein was only detected in pEGFP-transfected cells but not pEGFP-JEVE transfected cells as well as the result that no increased expression of nuclear factor-kB was observed in pEGFP-JEVE transfected cells. These experimental results indicate the effects of JEVE protein in alleviating TNF-alpha mRNA expression that is associated with the increased p53-independent p21(Waf1/Cip1) expression and provide an explanation in the role of JEV transmission through monocyte.
authors.affiliation: Institute of Immunology, Chung Shan Medical University, Taichung, Taiwan, Republic of China.
authors.forename: Tsai-Ching
authors.initials: TC
authors.lastname: Hsu
authors.forename: Ji-Qiang
authors.initials: JQ
authors.lastname: Gao
authors.forename: Ko-Hsiu
authors.initials: KH
authors.lastname: Lu
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Tsai
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.forename: Bor-Show
authors.initials: BS
authors.lastname: Tzang
mesh_terms: D000818:Animals; D002460:Cell Line; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D015536:Down-Regulation; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C493957:Cdkn1a protein, mouse; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016328:NF-kappa B; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D016159:Tumor Suppressor Protein p53; D014759:Viral Envelope Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins