title: Plasmacytoid dendritic cells count in antiretroviral-treated patients is predictive of HIV load control independent of CD4+ T-cell count.
abstract: The depletion in circulating dendritic cells (DCs) and inverse correlation with viral load have been described in human immunodeficiency virus (HIV)-infected patients. The aim of this study was to investigate whether the DC blood count in antiretroviral-treated patients might be predictive of viral load control independent of CD4+ T cell count. Plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) were enumerated using a newly developed flow cytometric assay based on TruCOUNT. A significant reduction of circulating pDCs and mDCs was detected both in untreated and -treated subjects. The probability of experiencing viral load increase according to pDC, and CD4 count at baseline was evaluated in 39 treated patients. Individuals with lower baseline pDCs were more likely to have an increase of HIV-RNA during the 30 month follow-up in comparison with patients with high pDCs (p <0.001). In particular, the pDC measurement may be useful in the context of a high CD4 count, to distinguish the patients who have virological failure despite high CD4 counts. These findings suggest that in treated patients the enumeration of circulating DCs, especially pDC count, can augment the predictive value of CD4 measurement in predicting virologic failure.
authors.affiliation: Department of Infectious and Tropical Diseases, Sapienza University, Polo Pontino, Latina, Italy.
authors.forename: Miriam
authors.initials: M
authors.lastname: Lichtner
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rossi
authors.forename: Maria C
authors.initials: MC
authors.lastname: Rizza
authors.forename: Fabio
authors.initials: F
authors.lastname: Mengoni
authors.forename: Ilaria
authors.initials: I
authors.lastname: Sauzullo
authors.forename: Anna P
authors.initials: AP
authors.lastname: Massetti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Luzi
authors.forename: Anne
authors.initials: A
authors.lastname: Hosmalin
authors.forename: Claudio M
authors.initials: CM
authors.lastname: Mastroianni
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Vullo
mesh_terms: D000328:Adult; D000368:Aged; D044966:Anti-Retroviral Agents / Q000494:pharmacology*; D023241:Antiretroviral Therapy, Highly Active; D015415:Biomarkers / Q000097:blood; D018791:CD4 Lymphocyte Count; D016022:Case-Control Studies; D002452:Cell Count / Q000379:methods*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000821:virology; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012367:RNA, Viral / Q000097:blood / Q000187:drug effects; D019562:Viral Load
chemical_list: D044966:Anti-Retroviral Agents; D015415:Biomarkers; D012367:RNA, Viral