Synthesis of multifunctional polyvinylsaccharide containing controllable amounts of biospecific ligands.
In the present study, the attempt to synthesize a multibiofunctional polymeric vector to be used for construction of composite scaffolds for bone tissue engineering has been undertaken. The polymers based on 2-deoxy-2-methacrylamido- d-glucose were functionalized by a growth factor (BMP-2), GRGDSP peptide, and poly( l-lysine) using aldehyde chemistry. The covalent modification process was quantitatively studied, and a polymer conjugate containing all these ligands was formed. In addition, the impacts of coupled ligands toward the adsorption of polymers on the commercial mineral macroporous matrix Sponceram used in cell culture applications were studied.
Korzhikov
V
V
Institute of Macromolecular Compounds, Russian Academy of Science, Bolshoy pr. 31, 199004, St. Petersburg, Russia.
Roeker
S
S
Vlakh
E
E
Kasper
C
C
Tennikova
T
T
D016475:3T3 Cells; D000327:Adsorption; D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008024:Ligands; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D011145:Polyvinyls / Q000138:chemical synthesis* / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013816:Thermodynamics; D013844:Thiazoles; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000737:chemistry
D000447:Aldehydes; C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D007202:Indicators and Reagents; D008024:Ligands; D009842:Oligopeptides; D011134:Polysaccharides; D011145:Polyvinyls; D013778:Tetrazolium Salts; D013844:Thiazoles; D016212:Transforming Growth Factor beta; D011107:Polylysine; C047981:arginyl-glycyl-aspartic acid; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic; C022616:thiazolyl blue