title: Stability of isoflavone daidzein and genistein in riboflavin, chlorophyll b, or methylene blue photosensitization.
abstract: Effects of photosensitizers including riboflavin, chlorophyll b, or methylene blue on the stability of daidzein and genistein were studied in model systems by high-performance liquid chromatography (HPLC). Concentration of daidzein and genistein in 80% methanol with riboflavin under light for 7 h was significantly decreased with the apparent 1st-order rate constants of 0.234 and 0.193/h, respectively, (P < 0.05), while those without riboflavin under light did not change significantly (P > 0.05). The stability of isoflavone aglycones in the dark did not change significantly irrespective of the presence of riboflavin (P > 0.05). The concentrations of daidzein and genistein in chlorophyll b or methylene blue model systems under light were not significantly different from those in the dark for 7 h (P > 0.05). Addition of sodium azide increased the stability of daidzein and genistein in riboflavin photosensitization with concentration dependent manner. However, the protective effects of beta-carotene addition on the photodegradation of isoflavones were not high. The stability difference of daidzein and genistein in riboflavin photosensitization may be due to the high reactivity of riboflavin through type I pathway, although singlet oxygen may be involved in part.
authors.affiliation: Dept. of Food Science and Technology, Seoul Natl. Univ. of Technology, Seoul, Korea.
authors.forename: Seungok
authors.initials: S
authors.lastname: Yang
authors.forename: Seungwook
authors.initials: S
authors.lastname: Lee
authors.forename: Hyun
authors.initials: H
authors.lastname: Chung
authors.forename: Jaehwan
authors.initials: J
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D002734:Chlorophyll / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D019833:Genistein / Q000737:chemistry; D007529:Isoflavones / Q000737:chemistry*; D008027:Light; D008751:Methylene Blue / Q000032:analysis*; D010084:Oxidation-Reduction; D010777:Photochemistry; D012256:Riboflavin / Q000032:analysis*
chemical_list: D000975:Antioxidants; D007529:Isoflavones; D002734:Chlorophyll; C037184:chlorophyll b; C004742:daidzein; D019833:Genistein; D008751:Methylene Blue; D012256:Riboflavin