title: Quantification of plasma reduced glutathione, oxidized glutathione and plasma total glutathione in healthy cats.
abstract: Glutathione is an important intracellular tripeptide with multiple functions. Abnormal glutathione metabolism is thought to play an important role in various diseases of cats. However, no data regarding concentration of plasma glutathione are available for domestic cats. This study discusses the development of a rapid, simple high pressure liquid chromatography method for measurement of reduced glutathione (GSH), oxidized glutathione (GSSH) and total glutathione (GSHt) in plasma, for the purpose of establishing baseline data for future studies. The mean concentrations of GSH, GSSH and GSHt were 4.51+/-1 microM; 19.44+/-3.79 microM (expressed as GSH equivalent) and 23.59+/-3.89 microM, respectively. This is the first report of plasma glutathione concentrations in this species.
authors.affiliation: Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina. laura@vet.unicen.edu.ar
authors.forename: Laura A
authors.initials: LA
authors.lastname: Denzoin
authors.forename: Rodolfo J
authors.initials: RJ
authors.lastname: Franci
authors.forename: Maria O
authors.initials: MO
authors.lastname: Tapia
authors.forename: Alejandro L
authors.initials: AL
authors.lastname: Soraci
mesh_terms: D000818:Animals; D002415:Cats / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005260:Female; D005978:Glutathione / Q000097:blood*; D019803:Glutathione Disulfide / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D008297:Male; D010949:Plasma / Q000378:metabolism*; D012016:Reference Values; D013045:Species Specificity
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione; D019803:Glutathione Disulfide