title: Antioxidant and hepatoprotective activities of Cirsium setidens Nakai against CCl4-induced liver damage.
abstract: The antioxidant activity and hepatoprotective potential of Cirsium setidens Nakai, a widely used medicinal plant, were investigated. The n-butanol (n-BuOH) fraction of leaves and roots of C. setidens had a higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than the other soluble fractions. The n-BuOH fraction of roots of C. setidens had a significant hepatoprotective activity at a dose of 500 mg/kg compared to that of a standard agent. The biochemical results were confirmed by histological observations indicating that C. setidens extract decreased ballooning degeneration in response to CCl(4) treatment. The n-BuOH fraction reduced CCl(4)-induced liver injury in rats, and transcript levels of genes encoding antioxidant enzymes such as glutathione peroxidase 1 (GPO1), glutathione peroxidase 3 (GPO3) and superoxide dismutase (SOD1) were elevated in the livers of rats treated with this fraction (500 mg/kg). Based on these results, we suggest that the C. setidens extract has hepatoprotective effect related to its antioxidant activity.
authors.affiliation: School of Biotechnology, Kangwon National University, Chuncheon, Kangwon-do, 200-701, South Korea.
authors.forename: Sung Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Seong-Il
authors.initials: SI
authors.lastname: Heo
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.forename: Min Jie
authors.initials: MJ
authors.lastname: Lee
authors.forename: Myeong-Hyeon
authors.initials: MH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002252:Carbon Tetrachloride Poisoning / Q000517:prevention & control*; D031180:Cirsium; D004247:DNA / Q000235:genetics; D000431:Ethanol; D016166:Free Radical Scavengers; D006834:Hydrazines; D008099:Liver / Q000187:drug effects / Q000473:pathology*; D008107:Liver Diseases / Q000517:prevention & control*; D000069463:Olive Oil; D010851:Picrates; D010936:Plant Extracts / Q000627:therapeutic use*; D010938:Plant Oils; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006834:Hydrazines; D000069463:Olive Oil; D010851:Picrates; D010936:Plant Extracts; D010938:Plant Oils; D000431:Ethanol; D004247:DNA; C004931:1,1-diphenyl-2-picrylhydrazyl