title: Histamine H1 receptors are expressed in mouse and frog semicircular canal sensory epithelia.
abstract: Histamine-related drugs are commonly used in the treatment of vertigo and related vestibular disorders. Their site and mechanism of action, however, are still poorly understood. To increase our knowledge of the histaminergic system in the vestibular organs, we have investigated the expression of H1 and H3 histamine receptors in the frog and mouse semicircular canal sensory epithelia. Analysis was performed by mRNA reverse transcriptase-PCR, immunoblotting and immunocytochemistry experiments. Our data show that both frog and mouse vestibular epithelia express H1 receptors. Conversely no clear evidence for H3 receptors expression was found.
authors.affiliation: Department of Physiological-Pharmacological Cellular-Molecular Sciences, University of Pavia, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Botta
authors.forename: Simona
authors.initials: S
authors.lastname: Tritto
authors.forename: Paola
authors.initials: P
authors.lastname: Perin
authors.forename: Umberto
authors.initials: U
authors.lastname: Laforenza
authors.forename: Giulia
authors.initials: G
authors.lastname: Gastaldi
authors.forename: Valeria
authors.initials: V
authors.lastname: Zampini
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Zucca
authors.forename: Stefano
authors.initials: S
authors.lastname: Valli
authors.forename: Sergio
authors.initials: S
authors.lastname: Masetto
authors.forename: Paolo
authors.initials: P
authors.lastname: Valli
mesh_terms: D000818:Animals; D054777:Hair Cells, Ampulla / Q000166:cytology / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D011893:Rana esculenta; D011969:Receptors, Histamine H1 / Q000235:genetics* / Q000378:metabolism*; D018100:Receptors, Histamine H3 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054776:Semicircular Ducts / Q000166:cytology / Q000378:metabolism*; D013045:Species Specificity; D014717:Vertigo / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D015837:Vestibular Diseases / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology
chemical_list: D011969:Receptors, Histamine H1; D018100:Receptors, Histamine H3; D006632:Histamine