Differential expression of system L amino acid transporters during wound healing process in the skin of young and old rats.
In order to elucidate the role of the system L-type amino acid transporters (LATs) in the wound healing process of aged and young subjects, we investigated the expression of LAT1, LAT2 and their subunit 4F2hc in the skin healing process after artificial wounds of dorsal skin in the young and old rats.


METHODS
The 1 cm full-thickness incisional wounds were made through the skin and panniculus carnosus muscle. The wounds were harvested at days 1, 3, 5 and 7 post-wounding, the experimental controls were harvested the skin of rat without wounds and the various analyses were performed.


RESULTS
In young rats, gradually and noticeable wound healing was detected, however, in old rats, wound healing was found to be greatly delayed. In young rats, the expression of LAT1 was increased rapidly on the day 1 after wound induction, on the other hand, in old rats, the expression of LAT1 after wound induction was not different from the control group. In young rats, the expression of LAT2 after the induction of wound was not different from the control group, however in old rats, the expression of LAT2 on the day 1 of wound induction was rapidly elevated.


CONCLUSIONS
These results suggest that the LAT1 and LAT2 increase in the wound healing process after cell injury in young and old rats, respectively.
Jeong
Moon-Jin
MJ
Oral Biology Research Institute and The Second Stage of BK21, Chosun University College of Dentistry, 375 Seosukdong, Dong-gu, Gwangju 501-759, Korea.
Kim
Chun Sung
CS
Park
Joo-Cheol
JC
Kim
Heung-Joong
HJ
Ko
Yeong Mu
YM
Park
Kyung Jin
KJ
Jeong
Soon-Jeong
SJ
Endou
Hitoshi
H
Kanai
Yoshikatsu
Y
Lim
Do-Seon
DS
Kim
Do Kyung
DK
D000375:Aging / Q000378:metabolism*; D027182:Amino Acid Transport System y+ / Q000096:biosynthesis*; D000818:Animals; D004195:Disease Models, Animal; D027301:Fusion Regulatory Protein 1, Light Chains / Q000096:biosynthesis*; D027283:Large Neutral Amino Acid-Transporter 1 / Q000096:biosynthesis*; D008297:Male; D021122:Protein Subunits; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing
D027182:Amino Acid Transport System y+; D027301:Fusion Regulatory Protein 1, Light Chains; D027283:Large Neutral Amino Acid-Transporter 1; D021122:Protein Subunits; C486582:Slc7a8 protein, rat