title: Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, produced by Streptomyces sp. K04-0144. I. Taxonomy, fermentation, isolation and structural elucidation.
abstract: Cyslabdan, a new potentiator of imipenem activity against methicillin-resistant Staphylococcus aureus, was isolated from the culture broth of Streptomyces sp. K04-0144 by Diaion HP-20 and ODS column chromatographies and preparative HPLC. The structure of cyslabdan was elucidated by spectroscopic analyses including NMR. The compound has a labdane-type diterpene skeleton connecting with an N-acetylcysteine via thioether linkage.
authors.affiliation: Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukumoto
authors.forename: Yong-Pil
authors.initials: YP
authors.lastname: Kim
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D004224:Diterpenes / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004357:Drug Synergism; D005285:Fermentation; D006801:Humans; D015378:Imipenem / Q000008:administration & dosage*; D009682:Magnetic Resonance Spectroscopy; D016106:Methicillin Resistance; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D013302:Streptomyces / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes; C529277:cyslabdan; D015378:Imipenem; D000111:Acetylcysteine