title: Comparative analysis of intestinal microbial community diversity between healthy and orally infected ducklings with Salmonella enteritidis by ERIC-PCR.
abstract: OBJECTIVE
  To analyze the difference of intestinal microbial community diversity between healthy and (S. enteritidis) orally infected ducklings.
  
  
  METHODS
  Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was applied to analyze the intestinal microbial community diversity and dynamic change including duodenum, jejunum, ileum, cecum and rectum from healthy ducklings and 7-day-old ducklings after oral infection with S. enteritidis at different time points.
  
  
  RESULTS
  The intestinal microbial community of the control healthy ducklings was steady and the ERIC-PCR band numbers of the control healthy ducklings were the least with rectum and were the most with caecum. ERIC-PCR bands of orally inoculated ducklings did not obviously change until 24 h after inoculation (p.i.). The numbers of the ERIC-PCR bands gradually decreased from 24 h to 72 h p.i., and then, with the development of disease, the band numbers gradually increased until 6 d p.i. The prominent bacteria changed because of S. enteritidis infection and the DNAstar of staple of ERIC-PCR showed that aerobe and facultative aerobe (Escherichia coli, Shigella, Salmonella) became preponderant bacilli in the intestine of orally infected ducklings with SE.
  
  
  CONCLUSIONS
  This study has provided significant data to clarify the intestinal microbial community diversity and dynamic change of healthy and S. enteritidis orally infected ducklings, and valuable insight into the pathogenesis of S. enteritidis infection in both human and animals.
authors.affiliation: Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China.
authors.forename: Sheng-Yan
authors.initials: SY
authors.lastname: Cao
authors.forename: Ming-Shu
authors.initials: MS
authors.lastname: Wang
authors.forename: An-Chun
authors.initials: AC
authors.lastname: Cheng
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Qi
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Yang
authors.forename: Shu-Xuan
authors.initials: SX
authors.lastname: Deng
authors.forename: Nian-Chun
authors.initials: NC
authors.lastname: Yin
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Deng-Chun
authors.initials: DC
authors.lastname: Zhou
authors.forename: De-Kang
authors.initials: DK
authors.lastname: Zhu
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Luo
authors.forename: Xiao-Yue
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004372:Ducks / Q000382:microbiology*; D017753:Ecosystem; D005243:Feces / Q000382:microbiology; D007422:Intestines / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial