[Compared studies on the effect and toxicity of extractions of Fructus Meliae Toosendan in mice].
OBJECTIVE
To evaluate the effect and acute toxicity of different extraction of Fructus Meliae Toosendan.


METHODS
Samples were extracted from Fructus Meliae Toosendan using petroleum ether (I), ethyl acetate (II), 80% alcohol (III) or water (IV). Miceś writhing response were induce by ip 0.9% acetic acid and foot ache were induced by sc formaldehyde. Mices feet or ears were smeated by sccarrageenin or dimethylbenzene. And the effects of these samples were evaluated on these models.


RESULTS
The sample II 40 g/kg significantly decreased the writhing response induced by acetic acid and relieved mice foot pain induced by formaldehyde, inhibited the swelling of mice ear induced by dimethylbenzene. It markedly decreased the body weight and increased the index of testes in mice. The sample III 20 g/kg inhibited foot swelling induced by carrageenin and ear swelling induced by dimethylbenzene in mice. The sample I significantly relieved the foot pain induced by formaldehyde, increased the indexes of testis and adrenal gland in mice. The sample IV had no action on pain or inflammatory in mice. The LD50 of the sample II was 82.85 g/kg. And ig the sample I 133.2 g/kg, sample III 122.0 g/kg or sample IV 52.0 g/kg could not induce mice to death.


CONCLUSIONS
The sample II has anti-inflammatory and antalgic action. But it also has serious acute toxicity. These results show that the efficacious component of Fructus Meilae Toosendan may exist in the ethyl acetate extraction, The toxicity and efficacious component may be the same material.
Cheng
Lei
L
Sichuan Institute of Chinese Materia Medica, Sichuan Chengdu 610041, China.
Lei
Yong
Y
Liang
Yuan-yuan
YY
Tang
Da-xuan
DX
Huang
Li
L
Tan
Zheng-huai
ZH
D000085:Acetates; D000700:Analgesics / Q000627:therapeutic use* / Q000633:toxicity; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use* / Q000633:toxicity; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D000431:Ethanol; D005260:Female; D005638:Fruit / Q000737:chemistry; D008297:Male; D031585:Melia / Q000737:chemistry*; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000188:drug therapy / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use* / Q000633:toxicity; D010946:Plants, Medicinal / Q000737:chemistry
D000085:Acetates; D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010936:Plant Extracts; D000431:Ethanol; C007650:ethyl acetate