title: High throughput gene expression measurement with real time PCR in a microfluidic dynamic array.
abstract: We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.
authors.affiliation: Fluidigm Corporation, South San Francisco, California, USA.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Spurgeon
authors.forename: Robert C
authors.initials: RC
authors.lastname: Jones
authors.forename: Ramesh
authors.initials: R
authors.lastname: Ramakrishnan
mesh_terms: D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D016133:Polymerase Chain Reaction