An intron loss of Dfak gene in species of the Drosophila melanogaster subgroup and phylogenetic analysis.
Drosophila focal adhesion kinase (Dfak) gene is a single-copy nuclear gene. Previous study revealed that Drosophila melanogaster and Drosophila simulans had lost an intron precisely within the tyrosine kinase (TyK) domain of this gene. However, this did not happen in several other Drosophila species, including Drosophila elegans, Drosophila ficusphila, Drosophila biarmipes, Drosophila jambulina, Drosophila prostipennis, Drosophila takahashii, and Drosophila pseudoobscura. In the current study, homologous sequences of Drosophila sechellia, Drosophila mauritiana, Drosophila yakuba, Drosophila teissieri, Drosophila santomea, and Drosophila erecta were amplified by polymerase chain reaction, and further sequencing analysis indicated that these species were missing a TyK domain intron, indicating they were closely related. The relationship of the D. melanogaster species group was reconstructed using TyK domain nucleotide sequences. The resulting phylogenetic tree revealed that these 8 species were the most related species in the melanogaster group. These results strongly support previously proposed classifications based on morphological and molecular data.
Zhan
Lei-Lei
LL
College of Life Science, Hubei University, Wuhan 430062, People's Republic of China.
Tian
Jun
J
Liu
Can
C
Ke
Fang
F
Yang
Yong
Y
Li
Chun-Xuan
CX
Qian
Yuan-Huai
YH
Zeng
Qing-Tao
QT
D000818:Animals; D004330:Drosophila / Q000145:classification* / Q000235:genetics; D004331:Drosophila melanogaster / Q000145:classification / Q000235:genetics*; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics*; D017353:Gene Deletion; D007438:Introns; D010802:Phylogeny; D017434:Protein Structure, Tertiary
D051416:Focal Adhesion Protein-Tyrosine Kinases