title: Changes in extracellular concentrations of some cytokines, chemokines, and neurotrophic factors after insertion of intracerebral microdialysis catheters in neurosurgical patients.
abstract: OBJECTIVE
  The extracellular levels of eight different inflammatory agents were analyzed during the initial 36 hours after insertion of microdialysis catheters in patients.
  
  
  METHODS
  Cerebral extracellular fluid from 38 patients who were treated in a neurosurgical intensive care unit for severe brain injury was collected every 6 hours for 36 hours. The concentration of interleukin (IL)-1 beta, IL-6, IL-8, macrophage inflammatory protein-1 beta, regulated on activation, normal T-cell expressed and secreted (RANTES), fibroblast growth factor-2, and vascular endothelial growth factor was determined by a multiplex assay, and IL-10 was determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  This is the first report regarding the presence of IL-10, IL-8, macrophage inflammatory protein-1 beta, regulated on activation, T-cell expressed and secreted, vascular endothelial growth factor, and fibroblast growth factor-2 in the tissue level proper of the living human brain. The study also provides new information regarding the response of IL-1 beta and IL-6 after insertion of a microdialysis catheter. The study confirms that the intriguing patterns of interplay between different components of the inflammatory response studied in laboratory settings are present in the human brain. This was most clearly observed in the variations in response between the three different chemokines investigated, as well as in the rapid and transient response of fibroblast growth factor-2.
  
  
  CONCLUSIONS
  The data presented illustrate the opportunity to monitor biochemical events of possible importance in the human brain and indicate the potential of such monitoring in neurosurgical intensive care. The study also underlines that any analysis of events in the brain involving mechanical invasiveness needs to take into account biochemical changes that are directly related to the manipulation of brain tissue.
authors.affiliation: Department of Neurosurgery, University Hospital, Linköping, Sweden. pekka.mellergard@lio.se
authors.forename: Pekka
authors.initials: P
authors.lastname: Mellergård
authors.forename: Oscar
authors.initials: O
authors.lastname: Aneman
authors.forename: Florence
authors.initials: F
authors.lastname: Sjögren
authors.forename: Pernilla
authors.initials: P
authors.lastname: Pettersson
authors.forename: Jan
authors.initials: J
authors.lastname: Hillman
mesh_terms: D001930:Brain Injuries / Q000601:surgery; D016207:Cytokines / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods; D045604:Extracellular Fluid / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D017551:Microdialysis / Q000379:methods; D009414:Nerve Growth Factors / Q000378:metabolism*; D009493:Neurosurgery / Q000379:methods; D011183:Postoperative Complications / Q000276:immunology* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D016207:Cytokines; D009414:Nerve Growth Factors