Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures.
Grapevine (Vitis vinifera L.) is subject to a number of diseases which affect yield and wine quality. After veraison, berries become strongly susceptible to pathogens due to different physiological changes including the accumulation of glucose and fructose, on the one hand, and to the decrease of anti-microbial compounds called stilbenes, on the other. To obtain berry protection, pesticides are excessively used leading to important cost to the grower and to undesirable environmental impact of the residues, especially in grape, soil and water. As a consequence, alternative strategies have to be developed. Exogenously applied biotic elicitors induce defense responses. We studied the effects of methyl jasmonate in combination with sucrose on defense-related gene expression, stilbene and anthocyanin production in grapevine cell suspensions. The methyl jasmonate/sucrose treatment was effective in stimulating phenylalanine ammonia lyase, chalcone synthase, stilbene synthase, UDP-glucose: flavonoid-O-glucosyltransferase, proteinase inhibitor and chitinase gene expression, and triggered accumulation of both piceids and anthocyanins in cells, and trans-resveratrol and piceids in the extracellular medium. Methyl jasmonate treatment might be an efficient natural strategy to protect grapevine berries in vineyard.
Belhadj
Assia
A
Groupe d'Etude des Substances Végétales à Activité Biologique, EA 3675, ISVV, Université Victor Segalen Bordeaux 2, UFR Sciences Pharmaceutiques, Bordeaux Cedex, France.
Telef
Nadège
N
Saigne
Cassandrine
C
Cluzet
Stéphanie
S
Barrieu
François
F
Hamdi
Saïd
S
Mérillon
Jean-Michel
JM
D000085:Acetates / Q000494:pharmacology*; D000217:Acyltransferases; D000872:Anthocyanins / Q000096:biosynthesis*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D003517:Cyclopentanes / Q000494:pharmacology*; D005632:Fructose / Q000378:metabolism; D005638:Fruit / Q000166:cytology / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D013549:Sweetening Agents / Q000494:pharmacology; D027843:Vitis / Q000166:cytology / Q000201:enzymology*; D014920:Wine
D000085:Acetates; D000872:Anthocyanins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D013267:Stilbenes; D013549:Sweetening Agents; D005632:Fructose; D013395:Sucrose; C072239:methyl jasmonate; D000217:Acyltransferases; C020141:flavanone synthetase; D005947:Glucose; D000077185:Resveratrol