title: Construction of recombinant mouse IgG1 antibody directed against varicella zoster virus immediate early protein 63.
abstract: Five varicella zoster virus (VZV) genes are known to be transcribed in latently infected human ganglia. Transcripts from VZV gene 63, which encodes an immediate early (IE) protein, are the most prevalent and abundant. To obtain a reagent that might facilitate studies of the role of the IE63 protein in latency and reactivation, we selected an IE63-specific Fab fragment from a phage library and used it to prepare a recombinant mouse IgG1 antibody that detects IE63 and functions in Western blot, immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assays.
authors.affiliation: Department of Neurology, University of Colorado School of Medicine, Denver, Colorado 80262, USA.
authors.forename: Niklaus H
authors.initials: NH
authors.lastname: Mueller
authors.forename: Laurie L
authors.initials: LL
authors.lastname: Graf
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Shearer
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Owens
authors.forename: Donald H
authors.initials: DH
authors.lastname: Gilden
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002644:Chickenpox / Q000276:immunology / Q000821:virology; D005814:Genes, Viral / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D019151:Peptide Library; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral; D007074:Immunoglobulin G; D019151:Peptide Library; D011994:Recombinant Proteins