title: Time course and spacial distribution of UV effects on human skin in organ culture.
abstract: Apoptosis plays an important role in eliminating cells from populations when cells have been exposed to UV irradiation and damaged. Studies of cells in culture have provided some details of the mechanisms involved when stress response genes act after exposure to UV irradiation and other environmental stresses. However, little is known about the responses of intact sections of human skin growing in organ culture to UV irradiation. In the work reported here, it was found that the response of organ-cultured human skin after exposure to UV irradiation is different than the response of cultured cells. At wavelengths below 300 nm, the action spectrum obtained from organ-cultured skin samples showed a lower sensitivity than that observed at 300 nm, indicating that the overlying stratum corneum and upper epidermal cell layers had probably caused a selective absorption of incident UV radiation at some wavelengths. At 3 hours after UV irradiation, p53 was phosphorylated at Ser 15 and Ser 46, and accumulated in the cell nuclei, notably after exposure to 280-320 nm wavelengths. Accumulations of Bax, active Caspase-3 and cleaved PARP were detected in apoptotic cells at 24 hours post-exposure, along with a reduction of Bcl-2 levels, notably after exposure to 300-365 nm light. This difference in apoptotic responses may result from the characteristics of the different irradiation wavelengths used, and from details in the skin's structure. The data obtained in this study using an organ-culture system utilized direct measurements of the biological effects of different wavelengths of UV lights.
authors.affiliation: Department of Biology, School of Medicine, Nara Medical University, Kashihara, Nara, Japan.
authors.forename: Eiichiro
authors.initials: E
authors.lastname: Mori
authors.forename: Akihisa
authors.initials: A
authors.lastname: Takahashi
authors.forename: Kou
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Saki
authors.initials: S
authors.lastname: Kakei
authors.forename: Daiki
authors.initials: D
authors.lastname: Tsujinaka
authors.forename: Megumi
authors.initials: M
authors.lastname: Unno
authors.forename: Shoko
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Ken
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Hatoko
authors.forename: Norio
authors.initials: N
authors.lastname: Murata
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Furusawa
authors.forename: Takeo
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000502:physiology; D005260:Female; D006801:Humans; D009924:Organ Culture Techniques; D012867:Skin / Q000528:radiation effects*; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D016159:Tumor Suppressor Protein p53