title: Evaluation of the in-vitro cidal activity and toxicity of a novel peroxygen biocide: 2-butanone peroxide.
abstract: The monomer of 2-butanone peroxide is a novel peroxygen derivative with potential use as biocide in the hospital environment. The aim of this study was to test the biocidal activity of different concentrations of the compound against American Tissue Culture Collection strains from 11 different micro-organisms, including bacteria, mycobacteria, spores, fungi and virus, following the European Standard guidelines. Toxicity tests were also carried out following United States Environmental Protection Agency Standards. 2-Butanone peroxide exhibited biocidal activity at 0.12% against Legionella pneumophila, at 0.5% against Escherichia coli, Pseudomonas aeruginosa and Enterococcus hirae, and at 1% against Staphylococcus aureus after 5 min contact at room temperature. Mycobactericidal activity was obtained at 0.5% after 60 min contact at 20 degrees C, and sporicidal activity was obtained at 4% after 60 min at 40 degrees C. Good fungicidal (against yeasts and moulds) and virucidal (adenovirus and poliovirus) activities were obtained at 0.5% after 60 min contact. Toxicity assessment showed negative results in the acute dermal irritation test, acute eye irritation test and acute oral toxicity test. The skin sensitisation test was negative. The safety profile in the toxicity tests and the basic cidal activity against the strains tested suggest that 2-butanone peroxide in the control of hospital infections.
authors.affiliation: Microbiology Department, School of Medicine, University of Valencia, Valencia, Spain. jglomas@retemail.es
authors.forename: J
authors.initials: J
authors.lastname: García-de-Lomas
authors.forename: M
authors.initials: M
authors.lastname: Lerma
authors.forename: L
authors.initials: L
authors.lastname: Cebrián
authors.forename: E
authors.initials: E
authors.lastname: Esteban
authors.forename: M-J
authors.initials: MJ
authors.lastname: Giménez
authors.forename: L
authors.initials: L
authors.lastname: Aguilar
authors.forename: V
authors.initials: V
authors.lastname: Domínguez
authors.forename: J J
authors.initials: JJ
authors.lastname: Randez
mesh_terms: D000818:Animals; D002074:Butanones / Q000633:toxicity*; D004202:Disinfectants / Q000494:pharmacology* / Q000633:toxicity*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006168:Guinea Pigs; D010545:Peroxides / Q000633:toxicity*; D011817:Rabbits; D018675:Toxicity Tests
chemical_list: D002074:Butanones; D004202:Disinfectants; D010545:Peroxides; C530000:2-butanone peroxide