title: [Serum types of enterovirus and clinical characteristics of 237 children with hand, foot and mouth disease in Shenzhen].
abstract: OBJECTIVE
  To study the clinical and epidemiologic characteristics and the serum types of enterovirus of hand, foot and mouth disease (HFMD) in children.
  
  
  METHODS
  The RT-nPCR method was established with universal primers within 5' untranslated region of enterovirus and VP1 region of Coxsackievirus A16 (CAV16) and enterovirus 71 (EV 71). Enteroviruses were detected with RT-nPCR in 237 children with HFMD. Clinical and epidemiologic characteristics and serum types of enterovirus of the patients with HFMD were studied.
  
  
  RESULTS
  The patients'age ranged from 7 months to 11 years (mean 4.2 +/- 0.5 years). The majority (94.5%) were less than 6 years old. HFMD was mostly seen in spring and winter (67.9%). Oral mucosal pox or ulcer as well as hand and foot rashes were observed in all 237 patients. Fever occurred in 141 patients (59.5%). Of the 237 patients, 133 (56.1%) were RT-nPCR positive. Of the 133 cases, 38 were positive for EV71, 64 were positive for CAV16, and 31 were negative for both EV71 and CAV16. The patients infected by different types of enteroviruses had similar clinical characteristics. Gene colon and sequence analysis for 12 strains of enteroviruses PCR positive products presented as EV71 (n=5), CAV16 (n=5), ECHO13 (n=1), and CAV5 (n=1).
  
  
  CONCLUSIONS
  HFMD tends to occur in younger children less than 6 years old. The majority are affected in spring and winter. EV71 and CAV16 are common pathogens of HFMD. There is no relationship between clinical characteristics and serum types of enteroviruses in HFMD patients.
authors.affiliation: Haifu Community Health Service Center, Eighth People's Hospital of Shenzhen, Shenzhen, Guangdong 518101, China.
authors.forename: Shou-Bin
authors.initials: SB
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Huang
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Tan
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Kan
authors.initials: K
authors.lastname: Chen
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Qiu
authors.forename: Si-Zhong
authors.initials: SZ
authors.lastname: Xing
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Liao
mesh_terms: D002648:Child; D002675:Child, Preschool; D004770:Enterovirus / Q000145:classification*; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D012703:Serotyping