title: Laser phototherapy effect on protein metabolism parameters of rat salivary glands.
abstract: The aim of this study was to evaluate the effects of infrared diode laser phototherapy (LP) on tissues of the submandibular gland (SMG) and parotid gland (PG). Wistar rats were randomly divided into experimental (A and B) and control (C) groups. A diode laser, 808 nm wavelength, in continuous wave mode, was applied to the PG, SMG and sublingual gland in the experimental groups on two consecutive days. The doses were 4 J/cm(2) and 8 J/cm(2), and total energy was 7 J and 14 J, respectively. The power output (500 mW) and power density (277 mW/cm(2)) were the same for both experimental groups. In order to visualize the area irradiated by the infrared laser, we used a red pilot beam (650 nm) with 3 mW maximum power for the experimental groups. For the control group, the red pilot beam was the only device used. The SMG and PG were removed after 1 week of the first irradiation. Total protein concentration, amylase, peroxidase, catalase and lactate dehydrogenase assays were performed, as well as histological analysis. Statistical tests revealed significant increase in the total protein concentration for groups A and B in the parotid glands (P < 0.05). Based on the results of this study, LP altered the total protein concentration in rats' parotid glands.
authors.affiliation: Oral Biology Research Center, Faculty of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo, SP, 05508-000, Brazil. lysimoes@usp.br
authors.forename: Alyne
authors.initials: A
authors.lastname: Simões
authors.forename: Walter Luiz
authors.initials: WL
authors.lastname: Siqueira
authors.forename: Marcelo Lazzaron
authors.initials: ML
authors.lastname: Lamers
authors.forename: Marinilce Fagundes
authors.initials: MF
authors.lastname: Santos
authors.forename: Carlos de Paula
authors.initials: Cde P
authors.lastname: Eduardo
authors.forename: José
authors.initials: J
authors.lastname: Nicolau
mesh_terms: D000818:Animals; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D008297:Male; D010544:Peroxidases / Q000378:metabolism; D010789:Phototherapy / Q000295:instrumentation*; D011879:Radiotherapy Dosage; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects*; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
chemical_list: D012471:Salivary Proteins and Peptides; D007770:L-Lactate Dehydrogenase; D010544:Peroxidases