title: Evaluation of the quality of processed blood salvaged during craniotomy.
abstract: BACKGROUND
  The objective of the study was to evaluate the quality of salvaged blood retrieved during craniotomy after the processing by autotransfusion devices and to compare the processing effects of 2 autotransfusion devices: ZITI-2000 (Jingjing Medical Equipment Limited, Beijing, PR China) and BRAT 2 (Cobe Cardiovascular Inc, Division of Sorin Biomedica Arvada Co, Arvada, Colo).
  
  
  METHODS
  Twenty-four patients scheduled for elective craniotomy were randomly allocated into 2 groups, and 1 of the 2 autotransfusion devices (ZITI-2000 and BRAT 2) was used for each group. The blood samples were collected, washed, and tested for select blood components. The efficacy of centrifugation and washing was assessed by determining the ER of PLTs, WBCs, K+, and fHb. The morphology of the washed erythrocytes and the 2-week-old PRBCs was evaluated by means of SEM.
  
  
  RESULTS
  Extensive hemolysis was observed in the collected blood of both ZITI-2000 and BRAT 2 groups, with fHb being 464.8 +/- 111.6 and 459.0 +/- 91.6 mg/dL, respectively. The ERs of PLTs, WBCs, K+, and fHb were >35%, >80%, >90%, and >90%, respectively. Although some shape-changed red cells were observed in the washed blood, discocytes were the dominant form in both groups, with the percentages of discocytes being 58.0% +/- 8.0% and 59.7% +/- 8.3%, respectively. A greater number of shape-changed red cells were found in 2-week-old PRBCs (P < .01).
  
  
  CONCLUSIONS
  For craniotomies, the quality of salvaged blood processed by both devices (ZITI-2000 and BRAT 2) is equally satisfactory. Although extensive hemolysis is noted in the salvaged blood, IBS can be effectively and safely used.
authors.affiliation: Department of Anesthesiology, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, People's Republic of China. yiqihunhun@sohu.com
authors.forename: Hui
authors.initials: H
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Dexiang
authors.initials: D
authors.lastname: Wang
authors.forename: Baoguo
authors.initials: B
authors.lastname: Wang
mesh_terms: D000465:Algorithms; D001772:Blood Cell Count; D001793:Blood Preservation; D001803:Blood Transfusion / Q000592:standards*; D001804:Blood Transfusion, Autologous / Q000592:standards*; D003399:Craniotomy; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006461:Hemolysis / Q000502:physiology; D006801:Humans; D008855:Microscopy, Electron, Scanning
chemical_list: D006454:Hemoglobins