Liposomes-entrapped chondroitin sulphate: ultrastructural characterization and in vitro biocompatibility.
The purpose of this study was ultrastructural characterization of liposomes-entrapped chondroitin sulphate and to prove their in vitro biocompatibility in a human dermal fibroblast culture system, in order to use liposome-entrapped chondroitin sulphate in the treatment of inflammatory disorders. Chondroitin sulphate entrapped in liposomes appears as electron-dense particles in ultra-thin section. Comparative studies using chondroitin sulphate, empty liposomes and liposome-chondroitin sulphate systems were performed in order to evaluate their effect on growth and morphology of fibroblasts after 48 h of culture. Light microscopy indicated that chondroitin sulphate, empty liposomes and liposome-chondroitin sulphate systems do not induce appreciable cytotoxic effects, and cells maintain normal morphology when compared to control fibroblasts.
Trif
Mihaela
M
Institute of Biochemistry, Splaiul Independentei 296, 060031 Bucharest, Romania.
Moldovan
Lucia
L
Moisei
Magdalena
M
Craciunescu
Oana
O
Zarnescu
Otilia
O
D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism* / Q000648:ultrastructure*; D016503:Drug Delivery Systems; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008859:Microscopy, Polarization
D008081:Liposomes; D002809:Chondroitin Sulfates