title: Molecular characterisation of Atlantic salmon paramyxovirus (ASPV): a novel paramyxovirus associated with proliferative gill inflammation.
abstract: Atlantic salmon paramyxovirus (ASPV) was isolated in 1995 from gills of farmed Atlantic salmon suffering from proliferative gill inflammation. The complete genome sequence of ASPV was determined, revealing a genome 16,968 nucleotides in length consisting of six non-overlapping genes coding for the nucleo- (N), phospho- (P), matrix- (M), fusion- (F), haemagglutinin-neuraminidase- (HN) and large polymerase (L) proteins in the order 3'-N-P-M-F-HN-L-5'. The various conserved features related to virus replication found in most paramyxoviruses were also found in ASPV. These include: conserved and complementary leader and trailer sequences, tri-nucleotide intergenic regions and highly conserved transcription start and stop signal sequences. The P gene expression strategy of ASPV was like that of the respiro-, morbilli- and henipaviruses, which express the P and C proteins from the primary transcript and edit a portion of the mRNA to encode V and W proteins. Sequence similarities among various features related to virus replication, pairwise comparisons of all deduced ASPV protein sequences with homologous regions from other members of the family Paramyxoviridae, and phylogenetic analyses of these amino acid sequences suggested that ASPV was a novel member of the sub-family Paramyxovirinae, most closely related to the respiroviruses.
authors.affiliation: National Veterinary Institute, Section for Fish Health, P.O. Box 8156 Dep., N-0033 Oslo, Norway. Knut.Falk@vetinst.no
authors.forename: K
authors.initials: K
authors.lastname: Falk
authors.forename: W N
authors.initials: WN
authors.lastname: Batts
authors.forename: A
authors.initials: A
authors.lastname: Kvellestad
authors.forename: G
authors.initials: G
authors.lastname: Kurath
authors.forename: J
authors.initials: J
authors.lastname: Wiik-Nielsen
authors.forename: J R
authors.initials: JR
authors.lastname: Winton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005393:Fish Diseases / Q000473:pathology / Q000821:virology; D016679:Genome, Viral; D005880:Gills / Q000473:pathology* / Q000821:virology; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000145:classification* / Q000235:genetics*; D018184:Paramyxoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D020317:Salmo salar / Q000821:virology*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins