Effects of grinding surgical tissue specimens and smear staining methods on Buruli ulcer microscopic diagnosis.
To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from patients suspected of having BU were analyzed. Both smear preparation methods and both staining methods were equivalent in any combination. Thus we recommend ZN stained smears of unground tissue for peripheral treatment centres.
Affolabi
Dissou
D
Laboratoire de Référence des Mycobactéries, Cotonou, Bénin, and Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
Bankolé
Honoré
H
Ablordey
Anthony
A
Hounnouga
Julie
J
Koutchakpo
Pierre
P
Sopoh
Ghislain
G
Aguiar
Julia
J
Dossou
Ange
A
Johnson
Roch Christian
RC
Anagonou
Séverin
S
Portaels
Françoise
F
D001431:Bacteriological Techniques; D001576:Benzophenoneidum; D054312:Buruli Ulcer / Q000175:diagnosis* / Q000601:surgery*; D004396:Coloring Agents; D004251:DNA Transposable Elements; D006801:Humans; D008853:Microscopy / Q000379:methods*; D019911:Mycobacterium ulcerans / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D040521:Subcutaneous Tissue / Q000382:microbiology*
D004396:Coloring Agents; D004251:DNA Transposable Elements; D012394:Rosaniline Dyes; D001576:Benzophenoneidum; C006898:carbol-fuchsin solution