title: Comparison of frequency-domain and time-domain fluorescence lifetime tomography.
abstract: We compare frequency-and time-domain formulations of deep-tissue fluorescence imaging of turbid media. Simulations are used to show that time-domain fluorescence tomography, implemented via the asymptotic lifetime-based approach, offers a significantly better separability of multiple lifetime targets than a frequency-domain approach. We also demonstrate experimentally, using complex-shaped phantoms, the advantages of the asymptotic time-domain approach over a Fourier-based approach for analyzing time-domain fluorescence data.
authors.affiliation: Athinoula A Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA. ankumar@nmr.mgh.harvard.edu
authors.forename: Anand T N
authors.initials: AT
authors.lastname: Kumar
authors.forename: Scott B
authors.initials: SB
authors.lastname: Raymond
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Bacskai
authors.forename: David A
authors.initials: DA
authors.lastname: Boas
mesh_terms: D003198:Computer Simulation; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014054:Tomography / Q000379:methods*