title: [Usefulness of PCR in rapidly diagnosing subcutaneous abscess and costal osteomyelitis caused by Mycobacterium bovis BCG].
abstract: Conventional polymerase chain reaction (PCR) used to identify mycobacterial species does not distinguish between Mycobacterium tuberculosis and M. bovis BCG, and several weeks or months may be needed to identify individual slow-growing Mycobacterial species. We report a 4-year-old girl who had subcutaneous abscess and sternal osteomyelitis after BCG vaccination at 4 month of age. We directly identified M. bovis BCG genome in the punctured abscess within a few days using PCR and PCR-restriction fragment length polymorphism. Such PCR is useful for rapidly diagnosing and managing of appropriate therapy in patients with infection due to M. bovis BCG.
authors.affiliation: Department of Pediatrics, Chiba University Graduate School of Medicine.
authors.forename: Naruhiko
authors.initials: N
authors.lastname: Ishiwada
authors.forename: Haruka
authors.initials: H
authors.lastname: Hishiki
authors.forename: Masaharu
authors.initials: M
authors.lastname: Watanabe
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ohkusu
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kohno
mesh_terms: D000038:Abscess / Q000175:diagnosis*; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D009163:Mycobacterium bovis / Q000235:genetics; D010019:Osteomyelitis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D012272:Ribs; D014376:Tuberculosis / Q000175:diagnosis*; D014394:Tuberculosis, Osteoarticular / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial