title: Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells.
abstract: Interactions between bone marrow-derived hematopoietic stem cells (BM-HSCs) and their local microenvironment are an integral part of signaling control of BM-HSCs migration, proliferation and differentiation. We hypothesized that both substrate topographical and biochemical cues promote BM-HSCs adhesive behaviors, which are crucial for BM-HSCs' homing, self-renewal and lineage commitment within their microenvironment. We employed electrospinning technique to fabricate nanofiber scaffolds (NFS) with poly(dl-lactide-co-glycolide) blended with collagen I. NFS was further coated with E-selectin, a critical adhesive biomolecule. Capture efficiency study showed that blended NFS, after coated with E-selectin, significantly increased cell capture percentage from 23.40% to 67.41% within 30 min and from 29.44% to 70.19% within 60 min of incubation at room temperature. This study highlights the potential of using a biomimetic scaffold to design a site-specific niche-like unit for facilitating proliferation or differentiation functions of BM-HSCs.
authors.affiliation: Graduate Program in Bioengineering, National University of Singapore, Singapore 117576, Singapore. g0500566@nus.edu.sg <g0500566@nus.edu.sg>
authors.forename: Kun
authors.initials: K
authors.lastname: Ma
authors.forename: Casey K
authors.initials: CK
authors.lastname: Chan
authors.forename: Susan
authors.initials: S
authors.lastname: Liao
authors.forename: William Y K
authors.initials: WY
authors.lastname: Hwang
authors.forename: Qi
authors.initials: Q
authors.lastname: Feng
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure; D004583:Electrons; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen