title: Marker rescue mapping of the combined Condit/Dales collection of temperature-sensitive vaccinia virus mutants.
abstract: Complementation analysis of the combined Condit/Dales collection of vaccinia virus temperature-sensitive mutants has been reported (Lackner, C.A., D'Costa, S.M., Buck, C., Condit, R.C., 2003. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305, 240-259), however not all complementation groups have previously been assigned to single genes on the viral genome. We have used marker rescue to map at least one representative of each complementation group to a unique viral gene. The final combined collection contains 124 temperature-sensitive mutants affecting 38 viral genes, plus five double mutants.
authors.affiliation: Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL, USA.
authors.forename: Sayuri E M
authors.initials: SE
authors.lastname: Kato
authors.forename: Nissin
authors.initials: N
authors.lastname: Moussatche
authors.forename: Susan M
authors.initials: SM
authors.lastname: D'Costa
authors.forename: Travis W
authors.initials: TW
authors.lastname: Bainbridge
authors.forename: Cindy
authors.initials: C
authors.lastname: Prins
authors.forename: Audra L
authors.initials: AL
authors.lastname: Strahl
authors.forename: Amber N
authors.initials: AN
authors.lastname: Shatzer
authors.forename: Alyson J
authors.initials: AJ
authors.lastname: Brinker
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Kay
authors.forename: Richard C
authors.initials: RC
authors.lastname: Condit
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002874:Chromosome Mapping; D005814:Genes, Viral; D005816:Genetic Complementation Test; D006358:Hot Temperature; D009154:Mutation; D017422:Sequence Analysis, DNA; D014616:Vaccinia virus / Q000235:genetics*; D010948:Viral Plaque Assay