Arabidopsis mitochondrial proteomics.
Significant efforts have sought to uncover the protein profile of Arabidopsis mitochondria to act as a model for the mitochondrial proteome from plants. A combination of techniques have been undertaken to achieve this goal. We outline a basic two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separation of mitochondrial proteins, in-gel trypsination techniques, complex protein lysate digestions, and the identification of proteins by matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) mass spectrometry.
Heazlewood
Joshua L
JL
ARC Centre of Excellence in Plant Energy Biology, School of Biomedical and Chemical Sciences, The University of Western Australia, Crawley.
Millar
A Harvey
AH
D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry*; D024101:Mitochondrial Proteins / Q000032:analysis / Q000737:chemistry; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D014357:Trypsin / Q000378:metabolism
D029681:Arabidopsis Proteins; D024101:Mitochondrial Proteins; D020543:Proteome; D014357:Trypsin