title: Low-intensity pulsed ultrasound accelerates periodontal wound healing after flap surgery.
abstract: OBJECTIVE
  A study was conducted to evaluate the effects of low-intensity pulsed ultrasound on wound healing in periodontal tissues after mucoperiosteal flap surgery.
  
  
  METHODS
  Bony defects were surgically produced bilaterally at the mesial roots of the mandibular fourth premolars in four beagle dogs. The flaps were repositioned to cover the defects and sutured after scaling and planing of the root surface to remove cementum. The affected area in the experimental group was exposed to low-intensity pulsed ultrasound, daily for 20 min, for a period of 4 wk from postoperative day 1 using a probe, 13 mm in diameter. On the control side, no ultrasound was emitted from the probe placed contralaterally. After the experiment, tissue samples were dissected out and fixed in 10% formalin for histological and immunohistochemical analyses.
  
  
  RESULTS
  The experimental group showed that the processes in regeneration of both cementum and mandibular bone were accelerated by low-intensity pulsed ultrasound compared with the control group. In addition, the expression level of heat shock protein 70 was higher in the gingival epithelial cells of the low-intensity pulsed ultrasound-treated tooth.
  
  
  CONCLUSIONS
  Our results suggest that osteoblasts, as well as cells in periodontal ligament and gingival epithelium, respond to mechanical stress loaded by low-intensity pulsed ultrasound, and that ultrasound accelerates periodontal wound healing and bone repair.
authors.affiliation: Department of Periodontology, Kanagawa Dental College, Yokosuka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ikai
authors.forename: T
authors.initials: T
authors.lastname: Tamura
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Itou
authors.forename: A
authors.initials: A
authors.lastname: Sugaya
authors.forename: S
authors.initials: S
authors.lastname: Iwabuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Mikuni-Takagaki
authors.forename: S
authors.initials: S
authors.lastname: Deguchi
mesh_terms: D016301:Alveolar Bone Loss / Q000601:surgery / Q000628:therapy*; D000818:Animals; D001861:Bone Regeneration; D025021:Cementogenesis; D003799:Dental Stress Analysis; D004285:Dogs; D005881:Gingiva / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D010006:Osteoblasts / Q000502:physiology; D013314:Stress, Mechanical; D013524:Surgical Flaps; D014464:Ultrasonic Therapy; D014945:Wound Healing
chemical_list: D018840:HSP70 Heat-Shock Proteins