title: Determination of total flavonoids in Scutellaria barbata D. Don by dynamic ultrasonic extraction coupled with on-line spectrophotometry.
abstract: A dynamic ultrasonic extraction (DUE) coupled with on-line detection by spectrophotometry is proposed for the determination of total flavonoids (calculated against scutellarin) in Scutellaria barbata D. Don. The extraction was performed in a common self-made extraction vessel put in an ultrasonic bath and a peristaltic pump was used to deliver the extraction solvent. Several experimental parameters of DUE, including flow rate and concentration of extraction solvent, ultrasonic power and sample amount, were optimized. In this work an on-line method was provided with the advantages of minimum sample amount (5 mg), on-line monitoring the extraction process and time saving (12.5 min). Compared with off-line method, the proposed method would be more convenient for obtaining continuous measurements and rapid optimization of the extraction process.
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Hu
authors.forename: Jingyan
authors.initials: J
authors.lastname: You
authors.forename: Changli
authors.initials: C
authors.lastname: Bao
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiangzhe
authors.initials: X
authors.lastname: Meng
authors.forename: Tingting
authors.initials: T
authors.lastname: Xiao
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Yutang
authors.initials: Y
authors.lastname: Wang
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
mesh_terms: D005419:Flavonoids / Q000032:analysis*; D015203:Reproducibility of Results; D031363:Scutellaria / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014465:Ultrasonics
chemical_list: D005419:Flavonoids; D012997:Solvents