title: The effect of photodynamic therapy on squamous cell carcinoma in a murine model: evaluation of time between intralesional injection to laser irradiation.
abstract: Successful treatment of naturally occurring periocular squamous cell carcinoma (SCC) in horses with photodynamic therapy (PDT) has been performed by injecting residual tumor with verteporfin and applying laser irradiation immediately following injection. This study used a murine model to evaluate the influence of time between intralesional injection of verteporfin to laser irradiation on tumor growth inhibition with PDT. Mice were randomized into six groups (n=10/group). Each tumor was injected with either 0.1mg/cm(3) of verteporfin (Tx) or 5% dextrose in water (C). Tx and C groups 1, 2, and 3 were irradiated at 1, 30, and 180min after injection. Wilcoxon-rank sum test (P< or =0.05) was performed to determine the relative change in tumor volume (RCTV) between groups. Statistical significance was demonstrated between treatment groups. Although verteporfin-PDT treated mice in Tx1 and Tx2 demonstrated a lower RCTV compared to C1 and C2 mice, the differences were not statistically significant.
authors.affiliation: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, 900 East Campus Drive, Columbia, MO 65211, USA.
authors.forename: Laura D
authors.initials: LD
authors.lastname: Barnes
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Giuliano
authors.forename: Juri
authors.initials: J
authors.lastname: Ota
authors.forename: Leah A
authors.initials: LA
authors.lastname: Cohn
authors.forename: Cecil P
authors.initials: CP
authors.lastname: Moore
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D005134:Eye Neoplasms / Q000188:drug therapy / Q000473:pathology / Q000662:veterinary*; D005260:Female; D051379:Mice; D010778:Photochemotherapy / Q000379:methods / Q000662:veterinary*; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000008:administration & dosage; D011897:Random Allocation; D018709:Statistics, Nonparametric; D013997:Time Factors; D016896:Treatment Outcome; D000077362:Verteporfin
chemical_list: D017319:Photosensitizing Agents; D011166:Porphyrins; D000077362:Verteporfin