Membrane raft disruption promotes axonogenesis in n2a neuroblastoma cells.
Membrane rafts are discrete microdomains found in cell membranes that contain cholesterol and glycosphingolipids such as gangliosides. As cholesterol is a major component of membrane rafts, its sequestration by the polyene filipin can be used to disrupt them. In previous work we observed that membrane raft disruption by filipin treatment of murine neuroblastoma N2a cells led to changes in expression of cell processes. In this study, we determined the type of process formation induced by filipin treatment as well as whether their expression was accompanied by changes in ganglioside content or subcellular distribution. The results indicate that the processes formed were axonal in nature and their expression was accompanied by changes in both ganglioside content as well as the subcellular localization of GM1.
Petro
Kimberly A
KA
Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA, 17033, USA.
Schengrund
Cara-Lynne
CL
D000818:Animals; D001369:Axons / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D005372:Filipin / Q000494:pharmacology*; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives / Q000096:biosynthesis; D021962:Membrane Microdomains / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D016501:Neurites / Q000502:physiology; D009447:Neuroblastoma / Q000378:metabolism / Q000503:physiopathology; D014407:Tumor Cells, Cultured
C064020:ganglioside GD1alpha; D005677:G(M1) Ganglioside; D005372:Filipin