[Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua].
OBJECTIVE
Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.


METHODS
A P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed.


RESULTS
The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.


CONCLUSIONS
The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.
Kong
Jian-Qiang
JQ
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Wang
Wei
W
Zheng
Xiao-Dong
XD
Zhu
Ping
P
Cheng
Ke-Di
KD
D000595:Amino Acid Sequence; D031172:Artemisia annua / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000145:classification / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal / Q000201:enzymology / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010940:Plant Proteins; D003577:Cytochrome P-450 Enzyme System