title: Enhanced smooth muscle cell adhesion and proliferation on protein-modified polycaprolactone-based copolymers.
abstract: Smooth muscle cells (SMC) were cultured for up to 6 days on copolymer films fabricated from a PCL-PEG-PCL block copolymer or P(epsilon-CL-co-D,L-LA)-PEG-P(epsilon-CL-co-D,L-LA), named P(100/0) and P(70/30), respectively. The films were modified by aminolysis using 1,6-hexanediamine, and fibronectin, fibrinogen, or fibrin layers were subsequently immobilized by physisorption or by covalent coupling using imidoester chemistry. Immobilization of all the tested proteins resulted in significantly enhanced cell adhesion on these polymers. Moreover, we found that covalently immobilized proteins supported significantly greater cell proliferation than physisorbed proteins over 6 days. SMC cultured on P(100/0) films modified by covalently attached fibronectin or fibrin layers proliferated at a rate comparable to that observed on control tissue culture polystyrene. The proposed surface modification schemes were much less efficient in improving cell attachment and proliferation on P(70/30) films. However, prewetting P(70/30) with a phosphate buffer prior to aminolysis significantly improved cell numbers following immobilization of fibronectin. Immunostaining of smooth muscle-specific alpha-actin of SMC grown on protein-modified P(100/0) 8 h and 48 h after cell seeding, confirmed preserved SMC phenotype on all modified surfaces.
authors.affiliation: Department of Chemical Engineering, Laboratoire de Bioingénierie et de Biophysique de l'Université de Sherbrooke, Université de Sherbrooke, Sherbrooke, Québec, Canada.
authors.forename: Hanna
authors.initials: H
authors.lastname: Bramfeldt
authors.forename: Patrick
authors.initials: P
authors.lastname: Vermette
mesh_terms: D000199:Actins / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology*; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000199:Actins; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D005337:Fibrin; D005340:Fibrinogen