Amperometric immunosensor for diagnosis of BLV infection.
A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.
Kurtinaitiene
Bogumila
B
Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania.
Ambrozaite
Dovile
D
Laurinavicius
Valdas
V
Ramanaviciene
Almira
A
Ramanavicius
Arunas
A
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D016583:Enzootic Bovine Leukosis / Q000175:diagnosis / Q000821:virology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification* / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000032:analysis*
D014759:Viral Envelope Proteins