title: Protein transduction of an antioxidant enzyme: subcellular localization of superoxide dismutase fusion protein in cells.
abstract: In protein therapy, it is important for exogenous protein to be delivered into the target subcellular localization. To transduce a therapeutic protein into its specific subcellular localization, we synthesized nuclear localization signal (NLS) and membrane translocation sequence signal (MTS) peptides and produced a genetic in-frame SOD fusion protein. The purified SOD fusion proteins were efficiently transduced into mammalian cells with enzymatic activities. Immunofluorescence and Western blot analysis revealed that the SOD fusion proteins successfully transduced into the nucleus and the cytosol in the cells. The viability of cells treated with paraquat was markedly increased by the transduced fusion proteins. Thus, our results suggest that these peptides should be useful for targeting the specific localization of therapeutic proteins in various human diseases.
authors.affiliation: Department of Biomedical Science and the Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Korea.
authors.forename: Dae Won
authors.initials: DW
authors.lastname: Kim
authors.forename: So Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Yeum Pyo
authors.initials: YP
authors.lastname: Lee
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Lee
authors.forename: Min Seop
authors.initials: MS
authors.lastname: Jeong
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Jang
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Kil Soo
authors.initials: KS
authors.lastname: Lee
authors.forename: Tae-Cheon
authors.initials: TC
authors.lastname: Kang
authors.forename: Moo Ho
authors.initials: MH
authors.lastname: Won
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Oh-Shin
authors.initials: OS
authors.lastname: Kwon
authors.forename: Won Sik
authors.initials: WS
authors.lastname: Eum
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006540:Herbicides / Q000494:pharmacology; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D019913:Nuclear Localization Signals / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D010269:Paraquat / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D000975:Antioxidants; D006540:Herbicides; D019913:Nuclear Localization Signals; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D013482:Superoxide Dismutase; D010269:Paraquat