title: [A quantitative analysis of mitochondrial protein differential expressions in hydroxycamptothecin-treated hepatoma cells].
abstract: OBJECTIVE
  To investigate the differentially expressed mitochondrial proteins in hydroxycamptothecin (HCPT)-treated SMMC-7721 cells by using quantitative proteome.
  
  
  METHODS
  SMMC-7721 cell apoptosis was induced by HCPT and the mitochondria were isolated with a mitochondria isolation kit. Mitochondrial proteins labeled with a cleavable isotope-coded affinity tag were identified and quantified using two-dimensional liquid chromatography/tandem mass spectrometry.
  
  
  RESULTS
  Highly purified mitochondria were obtained. Seventy-four mitochondrial proteins, which were statistically significantly altered (P less than 0.05) in HCPT-treated cells, were identified and analyzed. A total of 42 proteins were significantly down-regulated, and 32 were up-regulated in the cells that responded to apoptosis. The functions of these proteins were likely involved in cell energy metabolism, nucleic acid translation and transcription, cytoskeleton, etc.
  
  
  CONCLUSIONS
  Our results about the information of differentially expressed mitochondrial proteins in HCPT-treated cells and the control cells will help to understand the mechanism by which HCPT induces cell apoptosis. The integrated techniques we used in this study will be helpful to the investigation of subcellular quantitative proteomics.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400016, China.
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Yan
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Fu
authors.forename: Zong-Yin
authors.initials: ZY
authors.lastname: Qiu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism
chemical_list: D024101:Mitochondrial Proteins; D020543:Proteome; C527042:hydroxycamptothecinum; D002166:Camptothecin