title: Differential induction of protein expression and benzophenanthridine alkaloid accumulation in Eschscholtzia californica suspension cultures by methyl jasmonate and yeast extract.
abstract: Methyl jasmonate (MJ) and yeast extract (YE) induce protein expression and benzophenanthridine alkaloid accumulation in Eschscholtzia californica suspension cell cultures. One hundred microM MJ primarily induced dihydrosanguinarine 509.0+/-7.4 mg/l); 0.2 g/l YE induced sanguinarine (146.8+/- 3.8 mg/l) and an unknown compound. These results occur because dihydrobenzophenanthridine oxidase (DHBO) is induced by YE and not by MJ. YE and chitin (CHI) had similar effects on sanguinarine production and DHBO expression. Differential induction of secondary metabolites was shown in E. californica suspension cultures and the expression of proteins confirmed the metabolite results. Furthermore, treatment by various oligosaccharides helped us to understand the elicitation effect of YE in signal transduction pathways.
authors.affiliation: Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Hwa-Young
authors.initials: HY
authors.lastname: Cho
authors.forename: Hong Soon
authors.initials: HS
authors.lastname: Rhee
authors.forename: Sung-Yong H
authors.initials: SY
authors.lastname: Yoon
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Park
mesh_terms: D000085:Acetates / Q000032:analysis / Q000378:metabolism*; D053119:Benzophenanthridines / Q000032:analysis / Q000096:biosynthesis*; D018533:Biomass; D053898:Biosynthetic Pathways; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000032:analysis / Q000378:metabolism*; D031675:Eschscholzia / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D054883:Oxylipins / Q000032:analysis / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D015003:Yeasts / Q000378:metabolism*
chemical_list: D000085:Acetates; D053119:Benzophenanthridines; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; D010088:Oxidoreductases; C077698:dihydrobenzophenanthridine oxidase