Single-molecule diffusion study of activated EGFR implicates its endocytic pathway.
In this work, we have imaged the lateral diffusion of activated epidermal growth factor receptor (EGFR) on cell membrane for studying its internalization pathway. After EGF activation, the mobility of individual EGFR molecules was measured and compared with that in the cells disrupted of clathrin-coated pits and caveolae, the two endocytosis-competent membrane microdomains. The results implicated that activated EGFR molecules associated with clathrin-coated pits but not caveolae at low doses of EGF, whereas they were located in these two domains at high EGF doses. It provided supporting evidence for the occurrence of both clathrin-dependent and caveolae-dependent EGFR endocytosis.
Xiao
Zeyu
Z
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Wei
W
Yang
Yong
Y
Xu
Li
L
Fang
Xiaohong
X
D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D004705:Endocytosis / Q000502:physiology*; D066246:ErbB Receptors / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D015398:Signal Transduction / Q000502:physiology*
C512478:EGFR protein, human; D066246:ErbB Receptors