title: [Separation and regeneration of protoplast from Phellinus igniarius].
abstract: OBJECTIVE
  To study the conditions on separation and regeneration of protoplast from Phellinus igniarius.
  
  
  METHODS
  The effects of enzymolysis conditions of P. igniarius mycelia on yield of protoplast and culturing conditons on regeneration ratio of protoplast were investigated.
  
  
  RESULTS
  When the 8 days-old mycelia was hydrolysed by 1.5% of lywallzyme adding to driselase of 0. 5% and at 30 degrees C for 3 h and enzymolysis was stablized by sucrose as a stablisher of osmotic pressure, higher yield of P. igniarius protoplast was obtained. If 10 days-old mycelia was used as raw material of enzymolysis and manntol was selected as stablisher of osmotic pressure of enzymolysis, higher regeneration ratio of P. igniarius protoplast also would be obtained in following regeneration step at same time keeping higher yield. For the regeneration processing, it was beneficial for the regeneration of P. igniarius protoplast that PDA plusing mulberry ramulus was used as the culture medium of regeneration and manntol was selected as the osmotic pressure establisher of regeneration culture medium.
  
  
  CONCLUSIONS
  The method and conditions to keep both higher yield and regeneration ratio of P. igniarius protoplast were obtained.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zi-Ping
authors.initials: ZP
authors.lastname: Zhu
authors.forename: Hai-Le
authors.initials: HL
authors.lastname: Ma
mesh_terms: D003470:Culture Media / Q000494:pharmacology; D005656:Fungal Proteins / Q000494:pharmacology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D008353:Mannitol / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000494:pharmacology; D009997:Osmotic Pressure; D010447:Peptide Hydrolases / Q000494:pharmacology; D011124:Polyporaceae / Q000187:drug effects / Q000502:physiology*; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D009097:Multienzyme Complexes; C025310:lyticase; D008353:Mannitol; D013395:Sucrose; D006026:Glycoside Hydrolases; C035212:driselase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D010447:Peptide Hydrolases