Histamine H1 receptors are expressed in mouse and frog semicircular canal sensory epithelia.
Histamine-related drugs are commonly used in the treatment of vertigo and related vestibular disorders. Their site and mechanism of action, however, are still poorly understood. To increase our knowledge of the histaminergic system in the vestibular organs, we have investigated the expression of H1 and H3 histamine receptors in the frog and mouse semicircular canal sensory epithelia. Analysis was performed by mRNA reverse transcriptase-PCR, immunoblotting and immunocytochemistry experiments. Our data show that both frog and mouse vestibular epithelia express H1 receptors. Conversely no clear evidence for H3 receptors expression was found.
Botta
Laura
L
Department of Physiological-Pharmacological Cellular-Molecular Sciences, University of Pavia, Italy.
Tritto
Simona
S
Perin
Paola
P
Laforenza
Umberto
U
Gastaldi
Giulia
G
Zampini
Valeria
V
Zucca
Gianpiero
G
Valli
Stefano
S
Masetto
Sergio
S
Valli
Paolo
P
D000818:Animals; D054777:Hair Cells, Ampulla / Q000166:cytology / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D011893:Rana esculenta; D011969:Receptors, Histamine H1 / Q000235:genetics* / Q000378:metabolism*; D018100:Receptors, Histamine H3 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054776:Semicircular Ducts / Q000166:cytology / Q000378:metabolism*; D013045:Species Specificity; D014717:Vertigo / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D015837:Vestibular Diseases / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology
D011969:Receptors, Histamine H1; D018100:Receptors, Histamine H3; D006632:Histamine