title: Four acarviosin-containing oligosaccharides identified from Streptomyces coelicoflavus ZG0656 are potent inhibitors of alpha-amylase.
abstract: Four aminooligosaccharides were isolated and purified from the culture filtrate of Streptomyces coelicoflavus ZG0656. Their chemical structures were determined by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. The names acarviostatins I03, II03, III03, and IV03 were given to the oligomers due to their acarviosin core structures. Acarviostatins III03 and IV03, which contain three and four acarviosin-glucose moieties, respectively, were identified as novel compounds. The four acarviostatins were all mixed noncompetitive inhibitors of porcine pancreatic alpha-amylase (PPA). The inhibition constants (K(i)) for acarviostatins III03 and IV03 were 0.008 and 0.033muM, respectively. Acarviostatin III03 is the most effective alpha-amylase inhibitor known to date, with a K(i) value 260 times more potent than acarbose.
authors.affiliation: Department of Microbiology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Geng
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
mesh_terms: D020909:Acarbose / Q000737:chemistry; D000606:Amino Sugars / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D002384:Catalysis; D004791:Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000737:chemistry*; D013552:Swine; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000606:Amino Sugars; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; C076099:acarviosine; D000516:alpha-Amylases; D005947:Glucose; D020909:Acarbose