title: A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation.
abstract: Recent advances in tissue engineering technology suggest its application in different medical fields, including periodontology. There are some reports of new non-enzymatic methods of isolating human gingival fibroblast for short-time cultivation in vitro to be used in autologous gingival augmentation. The aim of this study was to obtain a simple and established method of culturing human gingival fibroblasts. The authors developed a recurrent method that can be successfully used in autologous gingival augmentation.
authors.affiliation: Department of Medical Biochemistry, Wroclaw Medical University, Wrocław, Poland. michal@bioch.am.wroc.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Saczko
authors.forename: Marzena
authors.initials: M
authors.lastname: Dominiak
authors.forename: Julita
authors.initials: J
authors.lastname: Kulbacka
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Chwiłkowska
authors.forename: Honorata
authors.initials: H
authors.lastname: Krawczykowska
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans