Transient infiltration of neutrophils into the thymus following whole-body X-ray irradiation in IL-10 knockout mice.
IL-10 is known to suppress the inflammatory responses in a variety of experimental models. Because we previously found that whole-body X-irradiation causes massive apoptosis in the thymus and transient infiltration of neutrophils, in this study, we examined whether or not IL-10 is involved in the regulation of neutrophil infiltration upon whole-body X-ray irradiation using IL-10 knockout mice. Although IL-10 was induced in the thymus on whole-body X-ray irradiation, apoptosis of thymocytes, neutrophil infiltration, and MIP-2 and KC production in the thymus were not affected by an IL-10 deficiency. Coculturing of bone marrow-derived macrophages with late apoptotic cells caused MIP-2 production, which was also not affected by an IL-10 deficiency. These results suggest the uniqueness of the inflammatory response induced by whole-body X-ray irradiation, which does not seem to be regulated by IL-10.
Fujiwara
Hiroya
H
Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1, Miyama, Funabashi, Japan.
Yamazaki
Takahiro
T
Uzawa
Akiko
A
Nagata
Kisaburo
K
Kobayashi
Yoshiro
Y
D000818:Animals; D017209:Apoptosis / Q000502:physiology* / Q000528:radiation effects*; D002478:Cells, Cultured; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018375:Neutrophil Activation / Q000502:physiology / Q000528:radiation effects; D009504:Neutrophils / Q000502:physiology* / Q000528:radiation effects; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism* / Q000528:radiation effects; D014916:Whole-Body Irradiation / Q000379:methods*
D016753:Interleukin-10