title: Development and adaptation of a multiprobe biosensor for the use in a semi-automated device for the detection of toxic algae.
abstract: Worldwide monitoring programs have been launched for the observation of phytoplankton composition and especially for harmful and toxic microalgae. Several molecular methods are currently used for the identification of phytoplankton but usually require transportation of samples to specialised laboratories. For the purpose of the monitoring of toxic algae, a multiprobe chip and a semi-automated rRNA biosensor for the in-situ detection of toxic algae were developed. Different materials for the electrodes and the carrier material were tested using single-electrode sensors and sandwich hybridisation that is based on species-specific rRNA probes. Phytoplankton communities consist of different species and therefore a biosensor consisting of a multiprobe chip with an array of 16 gold electrodes for the simultaneous detection of up to 14 target species was developed. The detection of the toxic algae is based on a sandwich hybridisation and an electrochemical detection method.
authors.affiliation: Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany. Sonja.Diercks@awi.de
authors.forename: Sonja
authors.initials: S
authors.lastname: Diercks
authors.forename: Katja
authors.initials: K
authors.lastname: Metfies
authors.forename: Linda K
authors.initials: LK
authors.lastname: Medlin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D056890:Eukaryota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008387:Marine Toxins / Q000032:analysis*; D008839:Microelectrodes; D015203:Reproducibility of Results; D012371:Robotics / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D014159:Transducers
chemical_list: D008387:Marine Toxins