[Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence quantitative PCR].
OBJECTIVE
To explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).


METHODS
The total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.


RESULTS
CCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).


CONCLUSIONS
CCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.
Zhang
Zhao-Xia
ZX
Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, China.
Cao
Li-Zhi
LZ
Huang
Qiong
Q
Yang
Ming-Hua
MH
Wang
Zhuo
Z
Yu
Yan
Y
D002648:Child; D056745:Cyclin C; D016213:Cyclins / Q000235:genetics*; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*
C503674:CCNC protein, human; D056745:Cyclin C; D016213:Cyclins