A drug-resistant leprosy case detected by DNA sequence analysis from a relapsed Mexican leprosy patient.
A skin biopsy sample was obtained from a relapsed lepromatous leprosy patient from the central area of Mexico. Genes associated with resistance to anti-leprosy drugs were analyzed by DNA sequence assay. A single nucleotide substitution was found at codon 53 (ACC-->GCC) in the folP gene, which is known to confer dapsone resistance. No mutations in the rpoB and gyrA, which indicate resistance to rifampicin and fluoroquinoles, were detected. This is the first reported case of dapsone resistant leprosy in Mexico in which the cause of the resistance is shown at genomic level. Evaluation of drug resistance by identifying known mutations in these genes by PCR is simple and reliable. Testing for resistance to anti-leprosy drugs should be performed in relapses or intractable cases for a better outcome.
Lopez-Roa
Rocio Ivette
RI
Centro de Investigación en Inmunología y Dermatología, Dpto. de Fisiología CUCS, Universidad de Guadalajara/Instituto Dermatológico de Jalisco, Guadalajara, Jalisco, México.
Fafutis-Morris
Mary
M
Masanori
Matsuoka
M
D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D003062:Codon / Q000235:genetics; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics*; D003622:Dapsone / Q000494:pharmacology* / Q000627:therapeutic use; D004094:Dihydropteroate Synthase / Q000235:genetics* / Q000502:physiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D006801:Humans; D007917:Leprostatic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015440:Leprosy, Lepromatous / Q000188:drug therapy / Q000382:microbiology*; D008800:Mexico; D009166:Mycobacterium leprae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D017354:Point Mutation; D012008:Recurrence; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D003062:Codon; D004269:DNA, Bacterial; D007917:Leprostatic Agents; D003622:Dapsone; D004094:Dihydropteroate Synthase