Improvement of transformation efficiency by bioactive-beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material.
The efficiency of bioactive-beads-mediated plant transformation was improved using DNA-lipofectin complex as the entrapped genetic material instead of naked DNA used in the conventional method. In the improved method, beads aggregated and formed clusters around the protoplasts resulting in a 4-fold higher transformation efficiency than that by the conventional method.
Murakawa
Tomoko
T
Sasebo National College of Technology, Sasebo, Nagasaki, Japan. murakawa@post.cc.sasebo.ac.jp
Kajiyama
Shin'ichiro
S
Ikeuchi
Tomohiko
T
Kawakami
Shigeki
S
Fukui
Kiichi
K
D017796:Caulimovirus / Q000235:genetics; D004247:DNA / Q000737:chemistry*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010944:Plants / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000737:chemistry / Q000378:metabolism; D014170:Transformation, Genetic
D010714:Phosphatidylethanolamines; D049452:Green Fluorescent Proteins; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA