T-251A polymorphism of IL-8 relating to the development of histological gastritis and G-308A polymorphism of TNF-alpha relating to the development of macroscopic erosion.
OBJECTIVE
Genetic variations of the inflammatory IL-8 and TNF-alpha genes can influence the outcome of gastric alterations. Our aims were to determine the prevalence and effect of the T-251A functional polymorphism of IL-8 and the G-308A polymorphism of TNF-alpha in histological and macroscopic gastric diseases related to Helicobacter pylori infection.


METHODS
Genomic DNA was extracted from biopsy samples from patients with gastritis (n=86, H. pylori positive=41), atrophy (n=32, H. pylori positive=13), intestinal metaplasia (IM) (n=43, H. pylori positive=22) and from histologically negative patients (n=57). The samples were divided by macroscopic diagnosis into erosion and negative groups. The T-251A polymorphism was examined with the amplification refractory mutation system method; the G-308A polymorphism was determined by the polymerase chain reaction-restriction fragment length polymorphism method. For statistical evaluation, Fischer's exact test was used.


RESULTS
In the case of T-251A of IL-8, the frequency of the A/A genotype was significantly increased in gastritis (P=0.049) and IM (P=0.038) groups as compared with the histologically negative ones. No relationship was found between macroscopic erosions and H. pylori infection. In the case of G-308A, the G/G genotype frequency was statistically increased in erosions as compared with negative groups (P=0.035). No difference in the distribution of G-308A genotypes in relation to histological alterations and the H. pylori infection was observed.


CONCLUSIONS
The effect of the polymorphism of IL-8 seems to be relevant in the pathogenesis of histological gastritis and IM, and the effect of the polymorphism of TNF-alpha is relevant in the pathogenesis of macroscopic erosive gastritis.
Szoke
Dominika
D
Department of Internal Medicine, Semmelweis University, Budapest, Hungary. szokedominika@yahoo.com
Molnar
Bela
B
Solymosi
Norbert
N
Klausz
Gergely
G
Gyulai
Zsofia
Z
Toth
Bernadett
B
Mandi
Yvette
Y
Tulassay
Zsolt
Z
D001284:Atrophy / Q000235:genetics / Q000382:microbiology; D005756:Gastritis / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications / Q000235:genetics / Q000473:pathology; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008679:Metaplasia / Q000235:genetics / Q000382:microbiology / Q000473:pathology; D011110:Polymorphism, Genetic; D013270:Stomach / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha