title: [Effect of different titanium surfaces on F-actin cytoskeleton of osteoblast].
abstract: OBJECTIVE To evaluate the effects of grooved, alkali- and heat-treated, acid-etched and TiO2 blasted surfaces of titanium substrates on F-actin cytoskeleton of osteoblasts in vitro.
  
  
  METHODS
  Osteoblasts derived from fetal rat calvarial were cultured on 6 different commercially pure titanium discs-grooved(G), sandblasted (SB), sand-blasted and acid-etching (SLA) surfaces and alkali- and heat-treated (AH1, AH2, AH3) surfaces. For F-actin cytoskeleton measurement, osteoblasts whose filamentous actin was stained with phalloidin-TRITC were cultured for 1, 2, 4, 12 h, evaluated by CLSM observation.
  
  
  RESULTS
  Osteoblasts attached to the different types of surfaces after 1 hour culture were similar. The actin cytoskeleton formed a ring of cortical filaments around the nucleus after 1 hour on SB, AH2, AH3, SLA surfaces. Actin filaments condensed along edges of pits. The actin filaments of seeded cells were spread after 2 h. The actin filaments on G formed bundles around the nucleus. The filaments began to parallel to the grooves. On AH1, the fibres formed a ring of cortical filaments around the nucleus with some cytoplasmic fibres radially oriented. On AH2, AH3, SB, the fibres orignised in a cytoplasmic meshwork with fibres which terminate at the ridge of depressions. The cell were suspending itself over the depressed areas. Actin filaments on SB were distinct and well formed that were oriented paralled to one another and the long axis of cells. After 4 h, actin filaments appeared organised in a parallel to one another and the long axis of cells. After 12 h, the actin filaments on all surfaces were well spread and were oriented paralled to another and to the long axis of the cell. The filaments formed bundles which reached to holes or adhered to the ridge of raised points, suspending cells over depressed areas.
  
  
  CONCLUSIONS
  After 12 h, the actin filaments on all surfaces were well spread and were oriented parallel to another and to the long axis of the cell. It was concluded that F-actin cytoskeleton of osteoblasts were spread best on SB surfaces among all surfaces.
authors.affiliation: Dept of Prosthodontics, College of Stomatology, Kunming Medical College, Kunming 650031, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Niu
authors.forename: Zhong-Juan
authors.initials: ZJ
authors.lastname: Ding
authors.forename: Fei
authors.initials: F
authors.lastname: Dong
mesh_terms: D008841:Actin Cytoskeleton; D000199:Actins; D000818:Animals; D003599:Cytoskeleton; D008870:Microtubules; D010006:Osteoblasts; D019736:Prostheses and Implants; D051381:Rats; D013499:Surface Properties; D014025:Titanium
chemical_list: D000199:Actins; D014025:Titanium