Detection of enteroviral RNA on Guthrie card dried blood of a neonate with fatal Coxsackie B3 myocarditis on day 17.
A fatal case of Coxsackie B3 myocarditis in a neonate is described. The clinical features became evident in the 3rd week of life, but enteroviral RNA was detected in the dried blood spot of the baby collected on day 4 after birth. This is the first report on the detection of enteroviral RNA in Guthrie card dried blood using reverse-transcriptase PCR. Materials and methods used are described in detail.
Smets
Koenraad
K
Department of Neonatology, Ghent University Hospital, De Pintelaan 185, B-9000 Gent, Belgium. koenraad.smets@ugent.be
Keymeulen
Annelies
A
Wollants
Elke
E
Lagrou
Katrien
K
Van Ranst
Marc
M
Padalko
Elizaveta
E
D001800:Blood Specimen Collection / Q000379:methods*; D003384:Coxsackievirus Infections / Q000175:diagnosis / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017809:Fatal Outcome; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D009205:Myocarditis / Q000175:diagnosis / Q000821:virology*; D012367:RNA, Viral / Q000097:blood* / Q000302:isolation & purification
D012367:RNA, Viral