Detection of Histoplasma capsulatum antigenuria by ultrafiltration of samples with false-negative results.
Patients with histoplasmosis may test falsely negative for Histoplasma capsulatum antigenuria. In some cases antigen is present at levels below the assay's detection limit, and ultrafiltration could improve sensitivity. Antigen was detected following ultrafiltration in 73.8% of falsely negative specimens versus 2% of controls. Ultrafiltration improved sensitivity with a small reduction in specificity.
Egan
Lindsey
L
MiraVista Diagnostics, 4444 Decatur Blvd., Suite 300, Indianapolis, IN 46241, USA. legan@miravistalabs.com
Connolly
Patricia A
PA
Fuller
Deanna
D
Davis
Thomas E
TE
Witt
John
J
Knox
Kenneth S
KS
Hage
Chadi A
CA
Wheat
L Joseph
LJ
D000946:Antigens, Fungal / Q000652:urine*; D005188:False Negative Reactions; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000276:immunology / Q000652:urine* / Q000821:virology; D006801:Humans; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods*
D000946:Antigens, Fungal