A high oocyte yield for intracytoplasmic sperm injection treatment is associated with an increased chromosome error rate.
OBJECTIVE
To compare the chromosome error rate among oocytes from stimulated ovaries after retrieval of 1-5 oocytes, 6-10 oocytes, and >10 oocytes.


METHODS
Retrospective cohort study.


METHODS
A university-based human genetic institute in collaboration with a private fertility center.


METHODS
Nine hundred thirty-three women undergoing intracytoplasmic sperm injection (ICSI) with a poor prognosis.


METHODS
Oocyte collection with ovarian stimulation. Polar body testing of ICSI oocytes for common chromosome errors.


METHODS
Chromosome error rate in oocytes, as determined by five-color fluorescence in situ hybridization.


RESULTS
In women less than 35 years and women between 35 and 40 years undergoing the first ICSI cycle, oocytes from the high-yield group had an increased likelihood for detectable chromosome errors (50.9% and 54.6%, respectively), compared to the intermediate-yield group (34.9% and 43.8%) and the low-yield group (23.3% and 41.2%). The overall high rate (>or=50%) of chromosomally abnormal oocytes in women more than 40 years appeared to be mainly due to the maternal age effect and increased only slightly with oocyte yield.


CONCLUSIONS
Oocyte yield may be considered as an indicator of ovarian response to hormone stimulation. In women up to 40 years a high yield of oocytes after superovulation is associated with an increased chromosome error rate.
Haaf
Thomas
T
Institute for Human Genetics, Epidemiology and Informatics, Johannes Gutenberg University Mainz, Mainz, Germany. haaf@humgen.klinik.uni-mainz.de
Hahn
Antje
A
Lambrecht
Anne
A
Grossmann
Bärbel
B
Schwaab
Eva
E
Khanaga
Omar
O
Hahn
Thomas
T
Tresch
Achim
A
Schorsch
Martin
M
D000328:Adult; D002869:Chromosome Aberrations / Q000139:chemically induced*; D015331:Cohort Studies; D005260:Female; D005300:Fertility Agents, Female / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008423:Maternal Age; D054315:Oocyte Retrieval; D010062:Ovulation Induction / Q000009:adverse effects*; D011247:Pregnancy; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome
D005300:Fertility Agents, Female