Viral protein synthesis is required for Enterovirus 71 to induce apoptosis in human glioblastoma cells.
Human glioblastoma cells (SF268) develop apoptosis, as characterized by DNA fragmentation and caspase activation, upon infection with Enterovirus 71 (EV71). To determine the step in virus replication that triggers apoptosis, the authors used ultraviolet (UV)-inactivated virus, inhibitors of protein and viral RNA synthesis, and chloroquine to block virus uncoating. Activation of caspase-3 was detected 24 h after infection with EV71 but not with UV-inactivated EV71. Apoptosis was inhibited when EV71-infected cells were treated with chloroquine, guanidine HCl, or cycloheximide. In summary, the authors studied the event(s) required to induce apoptosis in EV71-infected human glioblastoma cells, a subject much less studied than the possible role of viral proapoptotic genes, concluding that EV71 induces apoptosis in the infected SF268 cell in the presence of viral protein synthesis and virus replication, whereas virus adsorption, internalization, entry, uncoating, and viral RNA replication are all not required to trigger the apoptosis.
Shih
Shin-Ru
SR
Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Tao-Yuan, Taiwan.
Weng
Kuo-Feng
KF
Stollar
Victor
V
Li
Mei-Ling
ML
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor / Q000166:cytology / Q000821:virology; D002522:Chlorocebus aethiops; D002738:Chloroquine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D053938:DNA Fragmentation; D029821:Enterovirus A, Human / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D005909:Glioblastoma / Q000473:pathology; D019791:Guanidine / Q000494:pharmacology; D006801:Humans; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D014466:Ultraviolet Rays; D014709:Vero Cells / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D014764:Viral Proteins; D002738:Chloroquine; D003513:Cycloheximide; D019791:Guanidine