Proteome analysis of X-ray irradiated human erythroleukemia cells.
In order to discover molecular biomarkers in radiation response we investigated the effects of X-radiation on radioresistant K562 cells by using a comparative proteomic analysis. In treated cells 29 up-regulated and 10 down-regulated proteins were detected by image analysis and identified by mass spectrometry. Elongation factor 1 alpha 1 and stress-70 protein showed a 6.2 and 5.4 fold increase respectively in treated cells. Additional proteins such us pi and omega classes glutathione transferases, ATP synthase D chain, were also found to be up-regulated, suggesting that the enzyme belonging to the cellular detoxification system against oxidative stress and energetic metabolism may have a key role in the cellular response to radiation injury. This data set may provide a useful tool to design a combined chemo- and radiotherapic strategy against leukemia disease.
Eleuterio
Enrica
E
Department of Biomedical Science, G. d'Annunzio University, Chieti-Pescara, Italy.
Di Giuseppe
Fabrizio
F
Sulpizio
Marilisa
M
di Giacomo
Viviana
V
Rapino
Monica
M
Cataldi
Amelia
A
Di Ilio
Carmine
C
Angelucci
Stefania
S
D017209:Apoptosis / Q000528:radiation effects; D015153:Blotting, Western; D002453:Cell Cycle / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D002470:Cell Survival / Q000528:radiation effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology; D046529:Microscopy, Electron, Transmission; D020648:Peptide Elongation Factor 1 / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014965:X-Rays
D020648:Peptide Elongation Factor 1; D020543:Proteome; D005982:Glutathione Transferase