Genetic variability of genome segments 3 and 9 of Fiji disease virus field isolates.
Fiji leaf gall is an important disease of sugarcane in Australia and other Asia-Pacific countries. The causative agent is the reovirus Fiji disease virus (FDV). Previous reports indicate that there is variation in pathology between virus isolates. To investigate the amount of genetic variation found in FDV, 25 field isolates from Australia, Papua New Guinea and Malaysia were analysed by partial sequencing of genome segments S3 and S9. There was up to 15% divergence in the nucleotide sequence among the 25 isolates. A similar amount of divergence and pattern of relationships was found for each of the two genomic segments for most of the field isolates, although reassortment of genome segments seems likely for at least one of the Papua New Guinean isolates. The finding of a high level of variation in FDV isolated in different regions has implications for quarantine and disease management.
Jiang
Junxi
J
School of Integrative Biology, The University of Queensland, St Lucia, QLD 4072, Australia.
Ridley
Andrew W
AW
Tang
Herman
H
Croft
Barry J
BJ
Johnson
Karyn N
KN
D001315:Australia; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D016679:Genome, Viral; D008296:Malaysia; D008969:Molecular Sequence Data; D010219:Papua New Guinea; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D020133:Reverse Transcriptase Polymerase Chain Reaction; D031786:Saccharum / Q000821:virology; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D012367:RNA, Viral