Evaluation of serodiagnostic tests for T.b. gambiense human African trypanosomiasis in southern Sudan.
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood (99.5%). The specificity of CATT on diluted blood was similar (99.3%). The highest sensitivities (100%) were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood.
Elrayah
I E
IE
Trypanosomiasis Unit, Tropical Medicine Research Institute, Khartoum, Sudan. intisar62@yahoo.com
Rhaman
M A
MA
Karamalla
L T
LT
Khalil
K M
KM
Büscher
P
P
D000372:Agglutination Tests / Q000191:economics / Q000379:methods* / Q000592:standards; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000276:immunology; D016022:Case-Control Studies; D002555:Cerebrospinal Fluid / Q000469:parasitology; D003362:Cost-Benefit Analysis; D003430:Cross-Sectional Studies; D019353:Endemic Diseases / Q000706:statistics & numerical data; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods* / Q000592:standards; D006400:Hematocrit; D006801:Humans; D007841:Latex Fixation Tests / Q000191:economics / Q000379:methods* / Q000592:standards; D008196:Lymph / Q000469:parasitology; D008403:Mass Screening; D010274:Parasitology / Q000191:economics / Q000379:methods; D011159:Population Surveillance; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013397:Sudan / Q000453:epidemiology; D014347:Trypanosoma brucei gambiense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology
D000913:Antibodies, Protozoan