title: Renin inhibition activity by chitooligosaccharides.
abstract: Six kinds of chitooligosaccharides (COSs) with different molecular weight (MW) and degree of deacetylation (DD) were prepared using ultrafiltration membrane reactor, and their renin inhibition modes were evaluated. All the COSs showed the renin-inhibitory activities with dose-dependent manner, and 90-COSs had the potent renin-inhibitory activity than that of 50-COSs. Among them, 90-MMWCOS (1000-5000Da) exhibits the highest activity with IC(50) value of 0.51mg/mL and acts as competitive inhibitor with K(i) value of 0.28mg/mL by Lineweaver-Burk and Dixon plots. These results indicated that DD value and MW of COSs are important factors affecting renin-inhibitory activity.
authors.affiliation: Department of Biotechnology, Kunkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
mesh_terms: D000107:Acetylation; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000494:pharmacology*; D000092502:Renin Inhibitors
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002686:Chitin; D009844:Oligosaccharides; D000092502:Renin Inhibitors