Identification of herbarium whole-leaf samples of Epilobium species by ATR-IR spectroscopy.
A simple, high-accuracy FT-IR method based on attenuated total reflection (ATR) was developed for the rapid determination of leaf samples of Epilobium species. The method is superior to other analytical techniques, since there is no need of laborious sample preparation such as grinding or extraction and solvent removal. A total of 70 herbarium specimens, belonging to all 13 Epilobium and to 2 Chamerion species growing in Slovenia, were analyzed. With the 100 most-informative wavenumbers in the range 700-1800 cm(-1), we obtained over 90% accuracy of species identification, with discriminant multivariate statistical analysis on the measurements made on whole dried leaves.
Strgulc Krajsek
Simona
S
Department of Biology, Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, SI-1000 Ljubljana, Slovenia. simona.strgulc@bf.uni-lj.si
Buh
Primoz
P
Zega
Anamarija
A
Kreft
Samo
S
D016000:Cluster Analysis; D031666:Epilobium / Q000737:chemistry* / Q000145:classification*; D029594:Onagraceae / Q000737:chemistry / Q000145:classification; D018515:Plant Leaves / Q000737:chemistry*; D013045:Species Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods