[Effect of ventral prostate secretory proteins on oviductal fluid glycoproteins in golden hamsters].
OBJECTIVE
To investigate the effect of the secretory proteins of the ventral prostate on the glycoproteins in the oviductal fluid of golden hamsters.


METHODS
Male golden hamsters were divided into four groups: sham operation (SH), total removal of accessory sex glands (TX), and retainment of the ventral prostate only (VP). Oviductal fluid was collected from female hamsters at 0.5, 2, 4 and 6 h after mating with the males of different operated groups with or without ventral prostate. Glycoproteins were probed with a panel of lectins and their changes in the oviductal fluid were analyzed by Western blot.


RESULTS
The 47 000, 52 000, 81 000 and 128 000 WGA-binding proteins were observed in the oviductal fluid of the 6 h TX group, the 32 000, 35 500, 47 000 and 52 000 WGA-binding glycoproteins noted in the 6 h VP group, the 47 000, 68 000, 95 000 and 128 000 pisum sativum agglutinin (PSA)-binding glycoproteins shown in the 6 h TX and VP groups, two extra 32 000 and 37 500 bands detected in the 6 h VP group, the 47 000 and 52 000 dolichos biflorus agglutinin (DBA)-binding glycoproteins present in the 6 h VP but absent in the 6 h TX group.


CONCLUSIONS
Ventral prostate secretory proteins affect acetylglucosamine, N-acetylgalactosamine/galactose and mannose in the oviductal fluid collected 6 hours after mating. And these glycoproteins may play an important role in the development of embryos.
Luo
Jian-Min
JM
Lab for Neural Repair, Shantou University Medical College, Shantou, Guangdong 515031, China. jml@stu.edu.cn
Cheng
Lydia
L
Zhou
Yuan-Cong
YC
Chow
Patricia
P
D000818:Animals; D003307:Copulation / Q000502:physiology; D006224:Cricetinae; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D029584:Prostatic Secretory Proteins / Q000502:physiology*
D006023:Glycoproteins; D029584:Prostatic Secretory Proteins