title: Microbial changes in patients with acute periodontal abscess after treatment detected by PadoTest.
abstract: OBJECTIVE
  To investigate changes in bacterial counts in subgingival plaque from patients with acute periodontal abscess by IAI-PadoTest.
  
  
  METHODS
  Ninety-one patients were randomly allocated to either test or control groups. In all the patients, pockets with acute periodontal abscess were irrigated with sterilized physiological saline, and in the test group, 2% minocycline hydrochloride ointment was applied once into the pocket in addition. Subgingival plaque samples were collected by paper point before treatment and 7 days after treatment. The total bacterial count was determined and Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, were detected using IAI-PadoTest, a DNA/RNA probe method.
  
  
  RESULTS
  The total bacterial count decreased in both groups, with a significant decrease in the test group. The counts and number of sites positive for P. gingivalis, T. forsythia and T. denticola significantly decreased in the test group after treatment, compared with those in the control group. Pocket depth decreased in the both groups, with a statistically significant decrease in the test group.
  
  
  CONCLUSIONS
  Topical treatment with minocycline in pockets with acute periodontal abscess was effective in reducing the bacterial counts as shown by the microbiological investigation using PadoTest 4.5.
authors.affiliation: Health Care R&D Division, Sunstar Inc, Takatsuki-Osaka, Japan. tooru.eguchi@sunstar.com
authors.forename: T
authors.initials: T
authors.lastname: Eguchi
authors.forename: G
authors.initials: G
authors.lastname: Koshy
authors.forename: M
authors.initials: M
authors.lastname: Umeda
authors.forename: T
authors.initials: T
authors.lastname: Iwanami
authors.forename: J
authors.initials: J
authors.lastname: Suga
authors.forename: Y
authors.initials: Y
authors.lastname: Nomura
authors.forename: M
authors.initials: M
authors.lastname: Kawanami
authors.forename: I
authors.initials: I
authors.lastname: Ishikawa
mesh_terms: D000208:Acute Disease; D000287:Administration, Topical; D000368:Aged; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D015373:Bacterial Typing Techniques; D001439:Bacteroides / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D015169:Colony Count, Microbial; D015342:DNA Probes; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008911:Minocycline / Q000008:administration & dosage*; D010508:Periodontal Abscess / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D010514:Periodontal Pocket / Q000382:microbiology*; D015347:RNA Probes; D013357:Subgingival Curettage; D016896:Treatment Outcome
chemical_list: D000900:Anti-Bacterial Agents; D015342:DNA Probes; D015347:RNA Probes; D008911:Minocycline