Analysis of Staphylococcus aureus gene expression during PMN phagocytosis.
Staphylococcus aureus is a leading cause of human infections worldwide and causes a variety of diseases ranging in severity from mild to life-threatening. The ability of S. aureus to cause disease is based in part on its ability to subvert the innate immune system. Advances in genome-wide analysis of host-pathogen interactions have provided the necessary tools to investigate molecular factors that directly contribute to S. aureus pathogenesis. This chapter describes methods to analyze gene expression in S. aureus during interaction with human neutrophils.
Voyich
Jovanka M
JM
Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT, USA.
Sturdevant
Dan E
DE
DeLeo
Frank R
FR
D002478:Cells, Cultured; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000382:microbiology* / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010587:Phagocytosis / Q000502:physiology*; D013211:Staphylococcus aureus / Q000235:genetics*