title: CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein.
abstract: Little is known about vitamin D metabolism in fishes. Several reports have shown hydroxylase activities in various organs to produce vitamin D metabolites, but the enzymes involved have not been isolated or characterized. We isolated and characterized a renal mitochondrial hydroxylase, CYP27A1, that governs vitamin D metabolism in gilthead sea bream, Sparus auratus. The enzyme is highly expressed in kidney and to a far lesser extent in liver. When treated with 25-hydroxy vitamin D or calcitriol, the kidney responded differentially and time dependently with CYP27A1 mRNA expression levels. This response substantiates a role for CYP27A1 in fish vitamin D metabolism. This notion is strengthened by upregulation of CYP27A1 in sea bream treated with parathyroid hormone-related protein (PTHrP), and suggests an original role for PTHrP in calcitriol-regulated processes n fish similar to the role of PTH in mammalian vitamin D-dependent processes.
authors.affiliation: Department of Animal Physiology, Faculty of Science, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
authors.forename: Gideon S
authors.initials: GS
authors.lastname: Bevelander
authors.forename: Elsa S L C
authors.initials: ES
authors.lastname: Pinto
authors.forename: Adelino V M
authors.initials: AV
authors.lastname: Canario
authors.forename: Tom
authors.initials: T
authors.lastname: Spanings
authors.forename: Gert
authors.initials: G
authors.lastname: Flik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002117:Calcitriol / Q000494:pharmacology; D053493:Cholestanetriol 26-Monooxygenase / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008969:Molecular Sequence Data; D044162:Parathyroid Hormone-Related Protein / Q000494:pharmacology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D021541:Sea Bream / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014807:Vitamin D / Q000378:metabolism*; D014815:Vitamins / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D044162:Parathyroid Hormone-Related Protein; D012333:RNA, Messenger; D014815:Vitamins; D014807:Vitamin D; D053493:Cholestanetriol 26-Monooxygenase; D002117:Calcitriol