[Refinement study of macroporous resin for Huang-Lian-Jie-Du decoction].
OBJECTIVE
To study refinement of macroporous resin to Huang-Lian-Jie-Du decoction.


METHODS
The content changes of berberine, geniposide and total alkaloid in the solution which went through the macroporous resin were determined by TLC or spectrophotometry. The type of resin, absorption and desorption condition were optimized.


RESULTS
YWD-09D macroporous resin was suitable for refinement of Huang-Lian-Jie-Du decoction. The absorptive capacity of the resin was 1: 1.3. The concentration of solution was 0.4 g/ml, pH 4 and current velocity was 2 ml/min. The ratio of diameter and high of the resin column was 1:20. The desorption solution was 90% alcohol of 4BV.


CONCLUSIONS
YWD-09D macroporous resin and its selected condition are suitable for refinement of Huang-Lian-Jie-Du decoction.
Lin
Hui-bin
HB
Shandong Institute of TCM, Jinan 250014, China.
Lu
Ning
N
Yang
Jin-ping
JP
Lin
Jian-qun
JQ
Lin
Jian-qiang
JQ
D000327:Adsorption; D000470:Alkaloids / Q000032:analysis; D001599:Berberine / Q000032:analysis; D039323:Coptis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D032102:Phellodendron / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012117:Resins, Synthetic / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*
D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012117:Resins, Synthetic; D012997:Solvents; C061676:oren gedoku to; D001599:Berberine