title: Electrospray tandem mass spectrometric analysis of novel synthetic quinoxalinone derivatives.
abstract: Electrospray ionization tandem mass spectrometry (ESI-MS/MS) using a hybrid QqToF-MS/MS instrument has aided the structural characterization and differentiation of a novel series of medicinal synthetic 1-N-glycoside-quinoxalinone derivatives. These derivatives 7 and 8 are formed by an amino bond between the cyclic N-1 of the quinoxaline moiety and the C-6 position of a fully protected methyl or allyl alpha-D-mannofuranoside 3 and 4, and subsequent deprotection of the mannopyranoside moiety. In general the novel synthetic quinoxaline derivatives afforded the protonated molecules in ESI. The breakdown routes of the protonated molecules were rationalized by conducting low-energy CID-MS/MS analyses. In addition, re-confirmation of the various established fragmentation routes was achieved by conducting a series of ESI-CID-QqTof-MS/MS product ion scans on various selected precursor ions, which were initiated by CID in the atmospheric pressure/vacuum interface using a higher declustering potential. ESI-QqToF-MS/MS analysis has proven to be a specific and very sensitive method for the structural identification in the gas phase of these novel glycoquinoxalinamine derivatives.
authors.affiliation: Laboratoire de Physico-Chimie des Interfaces et Applications FRE CNRS 2485, Fédération Chevreul FR CNRS 2638, Site de Béthune, IUT de Béthune BP819, 62408 Béthune, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Joly
authors.forename: Chakir
authors.initials: C
authors.lastname: Jarmoumi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Massoui
authors.forename: El Mokhtar
authors.initials: el M
authors.lastname: Essassi
authors.forename: Patrick
authors.initials: P
authors.lastname: Martin
authors.forename: Joseph H
authors.initials: JH
authors.lastname: Banoub
mesh_terms: D011810:Quinoxalines / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D011810:Quinoxalines