title: Comparison of the Seeplex reverse transcription PCR assay with the R-mix viral culture and immunofluorescence techniques for detection of eight respiratory viruses.
abstract: This study evaluated the clinical usefulness of a newly introduced multiplex reverse transcription PCR assay (Seeplex RV; Seegene, Inc., Seoul, Korea) in patients with respiratory symptoms. Fifty clinical respiratory specimens (45 from children, 5 from adults) were tested for 8 viruses (influenza virus type A and B, parainfluenza virus type 1, 2, 3, respiratory syncytial virus type A and B, and adenovirus) by Seeplex RV (S-RV) and R-mix viral culture with immunofluorescence (VC-IF). Forty (80%) of the 50 samples showed concordant results between S-RV and VC-IF; 24 of these showed the same positive and 16 showed the same negative results. Among the 10 discrepant samples, 9 were S-RV-positive and VC-IF-negative. Six were obtained in patients with lower respiratory tract infection. Only 1 sample was VC-IF-positive and S-RV-negative. This patient had pneumonia. In 3 cases, more than 1 virus was identified by S-RV. The total running time of S-RV was 6 hr, which shortens the detection time for the viral presence by 2 workdays compared to VC-IF. In conclusion, S-RV is reliable, rapid, relatively easy to perform, and able to detect more than 1 virus simultaneously. Therefore, implementation of the S-RV assay in clinical laboratories will aid rapid diagnosis and treatment of major viral infections of the respiratory tract.
authors.affiliation: Department of Laboratory Medicine, Korea University Medical College, 126-1 Anam-dong, Sungbuk-gu, Seoul, Korea.
authors.forename: Kyoung Ho
authors.initials: KH
authors.lastname: Roh
authors.forename: Jeeyong
authors.initials: J
authors.lastname: Kim
authors.forename: Myung-Hyun
authors.initials: MH
authors.lastname: Nam
authors.forename: Sooyung
authors.initials: S
authors.lastname: Yoon
authors.forename: Chang Kyu
authors.initials: CK
authors.lastname: Lee
authors.forename: Kapno
authors.initials: K
authors.lastname: Lee
authors.forename: Young
authors.initials: Y
authors.lastname: Yoo
authors.forename: Min Ja
authors.initials: MJ
authors.lastname: Kim
authors.forename: Yunjung
authors.initials: Y
authors.lastname: Cho
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D018111:Pneumovirus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral