Altered serotonin 2C receptor RNA splicing in suicide: association with editing.
In an earlier study, we showed increases in serotonin 2C receptor (5-HT2CR) pre-mRNA editing in prefrontal cortex that were specific to suicide victims irrespective of associated psychiatric diagnoses. Here we demonstrate that the ratio between the two 5-HT2CR splice variants is increased in people who committed suicide, but does not vary among the diagnostic groups. This provides further evidence for suicide-specific neurobiology and suggests that, as it was previously shown in vitro, 5-HT2CR editing modulates its splicing in human brain. The association analysis indicates, however, that the efficiency of 5-HT2CR editing is an imperfect predictor of the splicing outcome, and that splice site selection is only partially controlled by the level of editing in vivo.
Dracheva
Stella
S
Department of Psychiatry, Mount Sinai School of Medicine, New York, New York, USA. Stella.Dracheva@mssm.edu
Chin
Benjamin
B
Haroutunian
Vahram
V
D000437:Alcoholism; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003851:Deoxyribonucleases / Q000737:chemistry; D006801:Humans; D017397:Prefrontal Cortex / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics*; D044405:Receptor, Serotonin, 5-HT2C / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013405:Suicide; D014158:Transcription, Genetic
D018076:DNA, Complementary; D044405:Receptor, Serotonin, 5-HT2C; D003851:Deoxyribonucleases