Taurine prevents tamoxifen-induced mitochondrial oxidative damage in mice.
Tamoxifen is a selective oestrogen receptor modulator widely used in the treatment of breast cancer. Tamoxifen potentially affects mitochondrial functions as it acts as an uncoupling agent and a powerful inhibitor of mitochondrial electron transport chain. There is concern for the deleterious effects of tamoxifen. Taurine is known to have membrane stabilizing and antioxidant properties. We studied effect of taurine pre-treatment on the toxicity of tamoxifen in mouse liver mitochondria focusing specifically on the redox cycle biomarkers. Tamoxifen caused a significant rise in the mitochondrial lipid peroxidation, protein carbonyl content and superoxide radical generation. There was a significant change in the mitochondrial thiol profile in the tamoxifen-treated animals. Pre-treatment of mice with taurine (100 mg/kg) markedly lowered mitochondrial lipid peroxidation, protein carbonyl content and superoxide radical generation. It also restored decreased enzymatic and non-enzymatic antioxidants of mitochondria. It is suggested that taurine has a potential role in ameliorating tamoxifen-induced mitochondrial toxicity, and the protection is afforded either by reversing the decline of antioxidants or by the direct free radical-scavenging activity.
Parvez
Suhel
S
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, India.
Tabassum
Heena
H
Banerjee
Basu Dev
BD
Raisuddin
Sheikh
S
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D020845:Selective Estrogen Receptor Modulators / Q000633:toxicity*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013629:Tamoxifen / Q000633:toxicity*; D013654:Taurine / Q000494:pharmacology*; D014475:Uncoupling Agents / Q000633:toxicity*
D000975:Antioxidants; D020845:Selective Estrogen Receptor Modulators; D013438:Sulfhydryl Compounds; D014475:Uncoupling Agents; D013629:Tamoxifen; D013481:Superoxides; D013654:Taurine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D019803:Glutathione Disulfide