Application of an in situ plated lead film electrode to the analysis of testosterone by adsorptive stripping voltammetry.
Adsorptive stripping voltammetry is a very sensitive and selective method for determination of drugs and organic substances in biological fluids. We have shown that determination of testosterone by adsorptive stripping voltammetry is possible using a lead film electrode. The lead film plating process and accumulation of testosterone were performed simultaneously from an acetate buffer solution of pH = 5.2 at a potential of -1.1 V. The measurements were carried out in undeaerated solutions. The detection limit was 9 x 10(-9) mol L(-1) for an accumulation time of 120 s; the relative standard deviation for 1 x 10(-7) mol L(-1) testosterone was 3.8%. The proposed voltammetric procedure for determination of testosterone could be applied to its determination in a pharmaceutical preparation and human urine samples directly without any separation steps.
Tyszczuk
Katarzyna
K
Faculty of Chemistry, Maria Curie Sklodowska University, 20-031, Lublin, Poland. ktyszczu@hermes.umcs.lublin.pl
D000327:Adsorption; D002138:Calibration; D004563:Electrochemistry; D004566:Electrodes; D007854:Lead / Q000737:chemistry*; D008567:Membranes, Artificial; D008968:Molecular Conformation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013739:Testosterone / Q000032:analysis*; D013997:Time Factors
D008567:Membranes, Artificial; D007854:Lead; D013739:Testosterone