A common idiotype in IgE and its relation to recognition of the grass pollen allergen Phl p 2.
The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a common idiotype is used to establish specific antibodies with a potential to mediate allergic responses to Phl p 2. Such a restriction may contribute to the limited molecular diversity observed in some IgE populations.
Persson
Helena
H
Department of Immunotechnology, Lund University, BMC D13, SE-221 84 Lund, Sweden.
Flicker
Sabine
S
Sadegh
Mardjaneh Karbalaei
MK
Greiff
Lennart
L
Valenta
Rudolf
R
Ohlin
Mats
M
D000485:Allergens / Q000276:immunology* / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D019295:Computational Biology; D018604:Epitope Mapping; D020869:Gene Expression Profiling; D006801:Humans; D007073:Immunoglobulin E / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D031784:Phleum / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology* / Q000378:metabolism; D011058:Pollen; D016415:Sequence Alignment
D000485:Allergens; D007130:Immunoglobulin Idiotypes; C084984:PHLPII protein, Phleum pratense; D010940:Plant Proteins; D007073:Immunoglobulin E