title: The -590 C/T and 8375 A/G interleukin-4 polymorphisms are not associated with Kawasaki disease in Taiwanese children.
abstract: Although some previous studies have reported that genetic and immunologic factors play important roles in the pathogenesis of Kawasaki disease (KD), the etiologic factors of this enigmatical pediatric disease are still poorly understood. This study aims to investigate whether polymorphisms of the interleukin-4 gene (IL-4; -590 C/T in the promoter region and 8375 A/G in intron 3) are associated with KD and the development of coronary artery lesions (CALs) in Taiwanese children. Genomic DNA was extracted from whole-blood samples from 150 children with KD and 472 ethnically matched healthy control subjects. The IL-4 -590 C/T and 8375 A/G single nucleotide polymorphisms (SNPs) were genotyped by a real-time polymerase chain reaction system with the Pre-Developed TaqMan Allelic Discrimination Assay. No significant associations between IL-4 SNPs and susceptibility of KD with CALs were found. In addition, no evidence for associations between IL-4 SNPs and CAL development was found. These results suggest that IL-4 -590 C/T and 8375 A/G SNPs do not confer a relevant role in the susceptibility or CAL development of KD in Taiwanese children.
authors.affiliation: Department of Pediatrics, Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: Huang
authors.forename: Tzu-Yang
authors.initials: TY
authors.lastname: Chang
authors.forename: Ming-Ren
authors.initials: MR
authors.lastname: Chen
authors.forename: Hung-Chang
authors.initials: HC
authors.lastname: Lee
authors.forename: Nan-Chang
authors.initials: NC
authors.lastname: Chiu
authors.forename: Hsin
authors.initials: H
authors.lastname: Chi
authors.forename: Chyong-Hsin
authors.initials: CH
authors.lastname: Hsu
authors.forename: Shuan-Pei
authors.initials: SP
authors.lastname: Lin
authors.forename: Hsin-Fu
authors.initials: HF
authors.lastname: Liu
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Chen
authors.forename: Chen-Chung
authors.initials: CC
authors.lastname: Chu
authors.forename: Marie
authors.initials: M
authors.lastname: Lin
authors.forename: Yann-Jinn
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D002648:Child; D002675:Child, Preschool; D003324:Coronary Artery Disease / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D009080:Mucocutaneous Lymph Node Syndrome / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013624:Taiwan
chemical_list: D015847:Interleukin-4