title: Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria.
abstract: The objective of the present study was to identify mitochondrial components associated with the damage caused by iron to the rat heart. Decreased cell viability was assessed by increased presence of lactate dehydrogenase (LDH) in serum. To assess the functional integrity of mitochondria, Reactive Oxygen Species (ROS), the Respiratory Control Ratio (RCR), ATP and chelatable iron content were measured in the heart. Chelatable iron increased 15-fold in the mitochondria and ROS increased by 59%. Deterioration of mitochondrial function in the presence of iron was demonstrated by low RCR (46% decrease) and low ATP content (96% decrease). Using two dimensional gel electrophoresis (2DE), we identified alterations in 21 mitochondrial proteins triggered by iron overload. Significantly, expression of the alpha, beta, and d subunits of F(1)F(o) ATP synthase increased along with the loss of ATP. This suggests that the F(1)F(o) ATP synthase participates in iron metabolism.
authors.affiliation: Division of Life Science, College of Natural Sciences, Sookmyung Women's University, Seoul, Korea.
authors.forename: Misun
authors.initials: M
authors.lastname: Kim
authors.forename: Jinsun
authors.initials: J
authors.lastname: Kim
authors.forename: Choong-Ill
authors.initials: CI
authors.lastname: Cheon
authors.forename: Dae Ho
authors.initials: DH
authors.lastname: Cho
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
authors.forename: Keun Il
authors.initials: KI
authors.lastname: Kim
authors.forename: Kyo-Young
authors.initials: KY
authors.lastname: Lee
authors.forename: Eunsook
authors.initials: E
authors.lastname: Song
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D002712:Chlorides; D015180:Electrophoresis, Gel, Two-Dimensional; D005290:Ferric Compounds / Q000494:pharmacology*; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D005343:Fibrinolytic Agents; D017382:Reactive Oxygen Species; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; C024555:ferric chloride