Reversal of the expression pattern of Aldolase C mRNA in Purkinje cells and Ube 1x mRNA in Golgi cells by a dopamine D1 receptor agonist injections in the methamphetamine sensitized-rat cerebellum.
The cerebellum has a parasagittal modular structure, in which Zebrin (Aldolase) positive and negative bands expressed in Purkinje cell layers alternate, and is involved in amphetamine psychosis. Administration of SKF38393, a D1 receptor agonist, reversed the behavioral sensitization of methamphetamine. In the vermis, there were the binding sites of SKF38393. In methamphetamine-sensitized rats the expression of the Aldolase mRNA positive bands move laterally in the rat vermis. We provide here the evidence that the D1 agonist injections also reversed the expression pattern of both the Aldolase mRNA in Purkinje cells and Ube (ubiquitin activating enzyme) 1x mRNA in Golgi interneurons of the sensitized rats. Thus the reverse changes in gene expression pattern in the vermis may be involved in the mechanisms of the behavioral plasticity and suggests the new treatment of drug abuse.
Hamamura
Mitsuko
M
Department of Fixed Prosthetics, Graduate School of Dental Sciences, Kyushu University, Fukuoka, Japan. mitsukohama@nifty.com
Hirata
Naotsugu
N
Sawada
Kazuhiko
K
Shuto
Takahide
T
Shimazoe
Takao
T
Terada
Yoshihiro
Y
Fukumaki
Yasuyuki
Y
D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Q000494:pharmacology; D000818:Animals; D001931:Brain Mapping; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D018491:Dopamine Agonists / Q000494:pharmacology*; D004347:Drug Interactions; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D008694:Methamphetamine / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics* / Q000378:metabolism
D018491:Dopamine Agonists; D012333:RNA, Messenger; D008694:Methamphetamine; D015647:2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine; D005634:Fructose-Bisphosphate Aldolase; D044764:Ubiquitin-Activating Enzymes