Olfactory ensheathing cells conditioned medium prevented apoptosis induced by 6-OHDA in PC12 cells through modulation of intrinsic apoptotic pathways.
Olfactory ensheathing cells (OECs) express a high level of growth factors which play a very important role as neuronal support. Recent evidence in literatures showed that transplantation of OECs may improve functional restoration in 6-OHDA-induced rat model of Parkinson's disease (PD). However, the biological function of various factors released from OECs in Parkinson' disease is still unclear. In this study, we examined the effects of newborn rat OECs conditioned medium (CM) on PC12 cells. Cells treated with 6-OHDA underwent cytotoxicity and apoptotic death determined by MTT assay and Hoechst 33342/PI staining. OECs CM was able to reduce the cellular damage in PC12 cells. Further investigation results showed that CM inhibited the disruption of mitochondrial transmembrane potential, up-regulation of Bcl-2 and down-regulation of Bax. Taken together, this study indicates that CM has a neuroprotective effect on 6-OHDA induced apoptosis of PC12 cells, which is through up-regulation of the Bcl-2/Bax ratio and protection for mitochondrion.
Feng
Lei
L
Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong, PR China.
Meng
Haiwei
H
Wu
Fengxia
F
Cheng
Baohua
B
He
Xiuquan
X
Wang
Xiaohong
X
Li
Zhengping
Z
Liu
Shuwei
S
D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001562:Benzimidazoles; D001681:Biological Assay; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D007202:Indicators and Reagents; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009498:Neurotoxins; D009830:Olfactory Bulb / Q000166:cytology / Q000378:metabolism*; D016627:Oxidopamine; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013778:Tetrazolium Salts; D013844:Thiazoles; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism
D001562:Benzimidazoles; D017077:Culture Media, Conditioned; D007202:Indicators and Reagents; D009414:Nerve Growth Factors; D009498:Neurotoxins; D019253:Proto-Oncogene Proteins c-bcl-2; D013778:Tetrazolium Salts; D013844:Thiazoles; D051028:bcl-2-Associated X Protein; D016627:Oxidopamine; C022616:thiazolyl blue; C017807:bisbenzimide ethoxide trihydrochloride