Application of primed in situ DNA synthesis (PRINS) with telomere human commercial kit in molecular cytogenetics of Equus caballus and Sus scrofa scrofa.
Recently, molecular techniques have become an indispensable tools for cytogenetic research. Especially, development of in situ techniques made possible detection at the chromosomal level, genes as well as repetitive sequences like telomeres or the DNA component of telomeres. One of these methods is primed in situ DNA synthesis (PRINS) using an oligonucleotide primer complementary to the specific DNA sequence. In this report we described application of PRINS technique with telomere human commercial kit to telomere sequences identification. This commercial kit may be use to visualization of interstitial telomeric signal in pig genome. PRINS is attractive complement to FISH for detection of DNA repetitive sequences and displays lower level of non-specific hybridization than conventional FISH.
Wnuk
Maciej
M
Department of Genetics, University of Rzeszów, Rzeszów, Poland. mawnuk@gmail.com
Bugno
Monika
M
Slota
Ewa
E
D000818:Animals; D033481:Chromosomes, Mammalian / Q000378:metabolism; D020732:Cytogenetic Analysis / Q000379:methods*; D006736:Horses / Q000235:genetics*; D006801:Humans; D008677:Metaphase; D020401:Primed In Situ Labeling / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D034421:Sus scrofa / Q000235:genetics*; D016615:Telomere / Q000378:metabolism*
D011933:Reagent Kits, Diagnostic