Analysis of the expression of biomarkers in urinary bladder cancer using a tissue microarray.
Dysregulation of Akt, PTEN, Drg-1, Cx-26, and L-plastin expression appear to be important in the progression of various cancers. Their expression in bladder cancer has not been well characterized. To assess the expression of these genes and their relationship to the outcome of bladder cancer, we used a bladder cancer tissue microarray (TMA) of 251 transitional cell carcinomas. We quantitated immunohistochemical staining of each protein using both automated and manual methods and correlated the expression levels with the clinicopathologic characteristics of the tumor and patient survival. Overall, the results from both automated and manual analyses were similar. We found a significant correlation between the expression of PTEN, Cx-26 and L-plastin with known clinically important pathologic features of bladder cancer (tumor grade, stage, and growth pattern). Aberrant localization patterns of Cx-26 and Drg-1 were observed in bladder tumors. There was also a significant correlation in expression among pAkt, PTEN, and L-plastin. Although the expression of these genes correlated with factors known to be associated with patient outcome, none of them was an independent predictor of progression-free or overall survival.
Harris
Loleta D
LD
Department of Urology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.
De La Cerda
Jorge
J
Tuziak
Tomasz
T
Rosen
Daniel
D
Xiao
Lianchun
L
Shen
Yu
Y
Sabichi
Anita L
AL
Czerniak
Bogdan
B
Grossman
H Barton
HB
D015415:Biomarkers / Q000032:analysis*; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D000072259:Connexin 26; D017630:Connexins / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D046228:Microarray Analysis; D009361:Neoplasm Invasiveness; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
D015415:Biomarkers; D017630:Connexins; D000072259:Connexin 26; C078418:developmentally regulated GTP-binding protein; D019204:GTP-Binding Proteins