title: PU.1 and CEBPA expression in acute myeloid leukemia.
abstract: Alterations of the transcription factors CCAAT/enhancer binding protein alpha (CEBPA) and PU.1 have been described in acute myeloid leukemia (AML). We studied CEBPA and PU.1 mRNA levels by real-time RT-PCR in 109 primary AML samples, compared with normal bone marrow and peripheral blood cells. Low PU.1 levels were observed in monoblastic leukemias, while low CEBPA levels were associated with leukopenia at diagnosis and lack of expression of differentiation antigens CD33 and CD11c. We conclude that down-regulation of CEBPA and PU.1 is not a general feature of primary AML, but appears to be restricted to distinct AML subtypes.
authors.affiliation: Institute of Hematology, Università Cattolica del Sacro Cuore, L.go A. Gemelli 8, Rome 00168, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Alò
authors.forename: Annalisa
authors.initials: A
authors.lastname: Di Ruscio
authors.forename: Francesco
authors.initials: F
authors.lastname: Guidi
authors.forename: Emiliano
authors.initials: E
authors.lastname: Fabiani
authors.forename: Mariangela
authors.initials: M
authors.lastname: Greco
authors.forename: Carlo
authors.initials: C
authors.lastname: Rumi
authors.forename: Stefan
authors.initials: S
authors.lastname: Hohaus
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Voso
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Leone
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D039521:CD11c Antigen / Q000235:genetics / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D007970:Leukopenia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D063268:Sialic Acid Binding Ig-like Lectin 3; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D000099232:Proto-Oncogene Protein Spi-1
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D022762:CCAAT-Enhancer-Binding Proteins; D039521:CD11c Antigen; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D063268:Sialic Acid Binding Ig-like Lectin 3; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C571163:CD33 protein, human; C515848:CEBPA protein, human