Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?
BACKGROUND
The aim of this study was to detect the expression of apoptosis factor caspase-3 in transferred HepG2 cells and provide feasible evaluation of the treatment for primary liver cancer with gene methods.


METHODS
The pcDNA4C-LIGHT cDNA was extracted from Escherichia coli JM-109; then, the pcDNA4C-LIGHT cDNA was transferred into the HepG2 cells by a cationic liposome mediated method. Meanwhile, the blank group was established as the control group and the HepG2 cells were collected after transfection at 12 h, 24 h, 48 h, 3 days and 5 days. The expression of caspase-3 was identified in the supernatants by ELISA. A standard curve was generated for the set of samples assayed. Statistical significance was analyzed by SPSS.


RESULTS
The quantity of caspase-3 protein was the greatest at 48 h and the least on day 5. The secretion of caspase-3 did not increase in the control group. The coefficient of correlation was equal to 0.9986 and had evident significance.


CONCLUSIONS
The pcDNA4C-LIGHT was effectively transfected in human HepG2 cells mediated by liposome. The expression of caspase-3 increased in the transfected group. This study provides necessary theoretic support for the treatment of liver cancer with gene methods.
Lu
Yun
Y
Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China. cloudylucn@126.com.cn
Wu
Li-Qun
LQ
Wang
Shou-Guang
SG
Lv
Zhen-Hua
ZH
Han
Bing
B
D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000628:therapy*; D053148:Caspase 3 / Q000096:biosynthesis / Q000235:genetics*; D045744:Cell Line, Tumor; D018076:DNA, Complementary / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005821:Genetic Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000628:therapy*; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000235:genetics*
D018076:DNA, Complementary; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14; D053148:Caspase 3