title: Evaluation of wound closure using different incision techniques with 23-gauge and 25-gauge microincision vitrectomy systems.
abstract: OBJECTIVE
  To describe the anatomic and histopathologic outcomes using different incision techniques with transconjunctival 23-G and 25-G vitrectomy systems.
  
  
  METHODS
  New Zealand rabbits were randomized to either 23-G or 25-G vitrectomy surgeries using angled incisions and straight incisions. After pars plana vitrectomy, the cannulas were removed and 0.1% trypan blue was injected to evaluate for leakage. The animals were killed on day 7 and the eyes enucleated for gross analysis and histopathologic analysis by frozen section.
  
  
  RESULTS
  Leakage of trypan blue was noted from 10.8% and 5.7% of straight and angled incisions, respectively. There was no difference between 23-G and 25-G incisions (8.3%). On gross examination, the 25-G system resulted in 58% and 24% open external wounds for straight and angled incisions, respectively (P = 0.04). The 23-G system resulted in 83% and 39% open external wounds with straight and angled incisions, respectively (P = 0.017). The average wound area after the 23-G surgery was 223.1 microm(2) and 115.7 microm(2) for straight versus angled incisions, respectively (P = 0.02). The average wound area formed after the 25-G surgery was 160.3 microm(2) and 85.2 microm(2) for straight versus angled incisions, respectively (P = 0.001).
  
  
  CONCLUSIONS
  Outcomes were similar for 23-G angled incisions, 25-G straight incisions, and 25-G angled incisions.
authors.affiliation: Cole Eye Institute, Cleveland Clinic Foundation, OH 44195, USA.
authors.forename: Rishi P
authors.initials: RP
authors.lastname: Singh
authors.forename: Hajime
authors.initials: H
authors.lastname: Bando
authors.forename: Oswaldo F M
authors.initials: OF
authors.lastname: Brasil
authors.forename: Dawn R
authors.initials: DR
authors.lastname: Williams
authors.forename: Peter K
authors.initials: PK
authors.lastname: Kaiser
mesh_terms: D000818:Animals; D004396:Coloring Agents; D003228:Conjunctiva; D008866:Microsurgery / Q000379:methods*; D011817:Rabbits; D012590:Sclera / Q000473:pathology / Q000601:surgery*; D013529:Surgical Wound Dehiscence / Q000175:diagnosis; D014343:Trypan Blue; D014821:Vitrectomy / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D004396:Coloring Agents; D014343:Trypan Blue