Boronate-containing polymer brushes: characterization, interaction with saccharides and mammalian cancer cells.
Boronate-containing polymer brushes were synthesized by free radical copolymerization of N,N-dimethylacrylamide (DMAA) and N-acryloyl-m-phenylboronic acid (NAAPBA) (9:1) on the surface of 3-mercaptopropyl-silylated glass plates and capillaries. The brushes were characterized with time-of-flight secondary ion mass-spectrometry (ToF SIMS), atomic force microscopy and contact angle measurements. Fructose caused a well-expressed drop spreading on the surface of copolymer-grafted glass, due to the strong interaction with the boronate groups. Sedimentation of murine hybridoma cells M2139 or human myeloid leukemia cells KG1 onto the DMAA-NAAPBA copolymer-grafted glass plates from 10 mM phosphate buffer solution (pH 8.0) resulted in the cell adhesion. The adhered M2139 and KG1 cells could be quantitatively detached from the grafted plates with 0.1 M fructose, which competed with cell surface carbohydrates for binding to the boronates. Evaluation of the binding strength between M2139 cells and the copolymer brush was performed by exposure of the adhered cells to a shear stress. Detachment of a fraction of 18% of the adhered M2139 cells was obtained at a shear force of 1400-2800 pN/cell generated by the running phosphate buffer (pH 8.0), whereas the remaining adhered cells (70%) could be detached with 0.1 M fructose dissolved in the same buffer. Possible applications of the boronate-containing polymer brushes to affinity cell separation can be based upon the facile recovery of the attached cells.
Ivanov
Alexander E
AE
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-22100, Lund, Sweden. Alexander.Ivanov@biotek.lu.se
Eccles
John
J
Panahi
Homayon Ahmad
HA
Kumar
Ashok
A
Kuzimenkova
Marina V
MV
Nilsson
Lars
L
Bergenståhl
Björn
B
Long
Nick
N
Phillips
Gary J
GJ
Mikhalovsky
Sergey V
SV
Galaev
Igor Yu
IY
Mattiasson
Bo
B
D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000473:pathology*; D011108:Polymers / Q000737:chemistry*
D001897:Boronic Acids; D002241:Carbohydrates; D011108:Polymers; C010686:benzeneboronic acid