Characterization of the Apc10/Doc1 subunit of the anaphase promoting complex in Drosophila melanogaster.
The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APC10/Doc1 subunit of the Drosophila APC/C. Loss of function APC10/Doc1 mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneuploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the Apc10/Doc1 subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in Drosophila.
Pál
Margit
M
Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary.
Varga
Kata
K
Nagy
Olga
O
Deák
P
P
D000483:Alleles; D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D001483:Base Sequence; D019926:Cyclin B / Q000378:metabolism; D017931:DNA Primers; D029721:Drosophila Proteins / Q000737:chemistry* / Q000235:genetics; D004331:Drosophila melanogaster; D005804:Genes, Lethal; D009154:Mutation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics; D043743:Ubiquitin-Protein Ligase Complexes / Q000737:chemistry* / Q000235:genetics
D019926:Cyclin B; D017931:DNA Primers; C476237:Doc1 protein, Drosophila; D029721:Drosophila Proteins; D014157:Transcription Factors; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome