Rapid and accurate identification of Candida albicans isolates by use of PNA FISHFlow.
We developed the simple, rapid (1 h), and accurate PNA FISH(Flow) method for the identification of Candida albicans. The method exploits unique in solution in situ hybridization conditions under which the cells are simultaneously fixed and hybridized. This method facilitates the accurate identification of clinical yeast isolates using two scoring techniques: flow cytometry and fluorescence microscopy.
Trnovsky
Jan
J
AdvanDx, Inc., 10A Roessler Road, Woburn, MA 01801, USA. jtr@advandx.com
Merz
William
W
Della-Latta
Phyllis
P
Wu
Fann
F
Arendrup
Maiken Cavling
MC
Stender
Henrik
H
D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008856:Microscopy, Fluorescence; D015341:Nucleic Acid Probes / Q000235:genetics*; D020135:Peptide Nucleic Acids; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; D011933:Reagent Kits, Diagnostic