Increasing voltage gradient electrophoresis of DNA.
We developed a method which allows electrophoretic fractionation of DNA in an agarose matrix according to an increasing current gradient, using a previously designed [R. Barbieri, V. Izzo, M.A. Costa, G. Giudice, G. Duro, Anal. Biochem. 212 (1993) 168; M.R. Asaro, V. Izzo, R. Barbieri, J. Chromatogr. A 855 (1999) 723] voltage gradient apparatus. This method allows the separation of different DNA fragments by increasing the distances of the components fractionated in the gel, revealing small differences in the length of different DNA components.
Oliva
Daniele
D
Istituto di Biomedicina e Immunologia Molecolare del CNR, Palermo, Italy.
Di Maio
Sabina
S
Sisino
Giorgia
G
Bellavia
Daniele
D
Barbieri
Rainer
R
D004247:DNA / Q000302:isolation & purification*; D004272:DNA, Mitochondrial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods*
D004272:DNA, Mitochondrial; D004247:DNA