N-methylpyridoxamine: novel canine vitamin B6 urine metabolite.
Cation-exchange HPLC analysis of urine from dogs given large daily doses of pyridoxamine revealed an unidentified metabolite hypothesized to be N-methylpyridoxamine. Identity was established by N-methylpyridoxamine synthesis and HPLC comparison to the canine metabolite. Compound synthesis was confirmed by IR, NMR, UV-vis and emission spectroscopy. It seems to have less fluorescent character than other routinely-measured vitamin B(6) metabolites. Upon administration of substantial pyridoxamine doses, N-methylpyridoxamine appears to be a quantifiable canine urine metabolite, although, at either pharmacological or dietary pyridoxamine intakes, its relevance to vitamin B(6) metabolism in other species, including humans, is not yet determined.
Ericson
Karen L
KL
Department of Chemistry, Indiana University-Purdue University Fort Wayne, 2101 E. Coliseum Boulevard, Fort Wayne, IN 46805-1499, USA. ericsonk@ipfw.edu
Maloney
Vincent M
VM
Mahuren
J Dennis
JD
Coburn
Stephen P
SP
Degenhardt
Thorsten P
TP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011733:Pyridoxamine / Q000031:analogs & derivatives* / Q000652:urine; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D025101:Vitamin B 6 / Q000378:metabolism*
C529306:N-methylpyridoxamine; D011733:Pyridoxamine; D025101:Vitamin B 6