title: Culture, isolation, and labeling of Anaplasma phagocytophilum for subsequent infection of human neutrophils.
abstract: Anaplasma phagocytophilum is the etiologic agent of granulocytic anaplasmosis, a tick-borne, zoonotic, emerging infectious disease. A. phagocytophilum is an obligate intracellular pathogen that primarily resides within membrane-bound, cytoplasmic vacuoles of host neutrophils. Closely related to Ehrlichial and Rickettsial organisms, A. phagocytophilum is a small, fragile, Gram-negative bacterium that presents unique challenges for culture, isolation, enumeration, and labeling. This chapter delineates pathogen-specific considerations for culture and labeling of this organism for subsequent use in assays to examine mechanisms of host cell-pathogen interactions.
authors.affiliation: Department of Pathology, Microbiology and Immunology, Section of Veterrinary Medicine, University of California, Davis, CA, USA.
authors.forename: Dori L
authors.initials: DL
authors.lastname: Borjesson
mesh_terms: D041081:Anaplasma phagocytophilum / Q000254:growth & development* / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D045744:Cell Line, Tumor; D018922:HL-60 Cells; D006801:Humans; D009504:Neutrophils / Q000382:microbiology*