Failed induction of heme oxygenase 1 in endothelial cells exposed to the hemoglobin based oxygen carrier Oxyglobin.
We investigated the effect of the bovine hemoglobin based oxygen carrier HBOC-200 (Oxyglobin) in rat aortic endothelial cells (RAEC) on the activation of heme oxygenase 1 (HO-1). RAEC were incubated in the presence of 75 microM (G1) or 225 microM (G2) HBOC-200. The positive control (G3) was performed by incubation with 50 microM Hemin. For negative control (G4) cells were incubated with medium alone. G2 and G3 reached a significant increase of bilirubin concentration compared to G4. A positive HO-1 signal in the Western Blot was seen in G3 12 and 24 h after incubation. The Western Blot of G1, G2 and G4 showed no HO-1 signal. These data suggest that HBOC-200 in the applied dosage cannot induce HO-1 expression in RAEC, and may be degraded by isoenzymes at a lower level.
Rempf
Christian
C
Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Knappschaftskrankenhaus Bochum Langendreeer, University Hospital, Bochum, Germany. rempf@anaesthesia.de
Ritter
Arno
A
Schrepfer
Sonja
S
Freitag
Marc
M
Standl
Thomas
T
Gottschalk
André
A
D000818:Animals; D001011:Aorta / Q000166:cytology; D001663:Bilirubin / Q000032:analysis / Q000096:biosynthesis; D001802:Blood Substitutes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006427:Hemin / Q000494:pharmacology; D006454:Hemoglobins / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors
D001802:Blood Substitutes; D006454:Hemoglobins; C402601:hemoglobin glutamer-200; D006427:Hemin; D051547:Heme Oxygenase-1; D001663:Bilirubin