title: Comparison of polymerase chain reaction methods, in situ hybridization, and enzyme immunoassay for detection of Chlamydia pneumoniae in atherosclerotic carotid plaques.
abstract: Chlamydia pneumoniae has been associated with cardiovascular diseases and has been shown by different methods to be present in atherosclerotic lesions. However, not all studies have found C. pneumoniae in atherosclerotic tissues. We compared polymerase chain reaction (PCR) methods, in situ hybridization (ISH), and measurement of chlamydial lipopolysaccharide (cLPS) by enzyme immunoassay (EIA) from homogenized atherosclerotic tissue in the detection of C. pneumoniae. In a study population of 110 patients with carotid artery disease, cLPS was found in 22.2%, and DNA by PCR was found in 34.3% and by ISH in 39.4% of the samples. Poor repeatability was shown to complicate PCR, and the technical problems inherent in ISH were not insignificant. In contrast, the cLPS EIA test was fast and easy to perform. If the sensitivity could be increased, for example, by testing multiple tissue pieces, cLPS EIA might provide a standardized commercial method for the detection of chlamydia in tissue samples, and it, thus, merits further characterization and validation in different patient populations.
authors.affiliation: Respiratory Infection Unit, National Public Health Institute, Oulu, Finland. taina.lajunen@ktl.fi
authors.forename: Taina
authors.initials: T
authors.lastname: Lajunen
authors.forename: Pirkka
authors.initials: P
authors.lastname: Vikatmaa
authors.forename: Tuija
authors.initials: T
authors.lastname: Ikonen
authors.forename: Mauri
authors.initials: M
authors.lastname: Lepäntalo
authors.forename: Kari
authors.initials: K
authors.lastname: Lounatmaa
authors.forename: Raija
authors.initials: R
authors.lastname: Sormunen
authors.forename: Aino
authors.initials: A
authors.lastname: Rantala
authors.forename: Maija
authors.initials: M
authors.lastname: Leinonen
authors.forename: Pekka
authors.initials: P
authors.lastname: Saikku
mesh_terms: D000368:Aged; D016893:Carotid Stenosis / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D008070:Lipopolysaccharides / Q000032:analysis; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D008070:Lipopolysaccharides