The bacterivorous ciliate Cyclidium glaucoma isolated from a sewage treatment plant: molecular and cytological descriptions for barcoding.
A strain of Cyclidium isolated from a sewage treatment plant in Geneva, Switzerland, was cloned and cultured under laboratory conditions before being characterized cytologically and molecularly. Information about the classical morphology and also about new molecular sequences has been obtained permitting its identification as Cyclidium glaucoma. The molecular description includes the 18S rRNA sequence, and for the first time for C. glaucoma the ITS-1, 5.8S, ITS-2 sequence, and the mitochondrial Cytochrome oxidase C subunit 1 sequence. Three divergence groups were identified for C. glaucoma using a short rDNA sequence for evolutionary distance analysis. These groups correspond to the three ribotype lineages previously described for C. glaucoma. We discuss the possibility that C. glaucoma is a species complex containing three cryptic species, the problems of species definition in the protozoa and the choice of a barcoding molecule for the ciliates.
Guggiari
Matteo
M
Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva 4, Switzerland.
Peck
Robert
R
D000818:Animals; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D003576:Electron Transport Complex IV / Q000235:genetics; D008969:Molecular Sequence Data; D016802:Oligohymenophorea / Q000145:classification / Q000166:cytology / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000469:parasitology*; D013045:Species Specificity; D014865:Waste Disposal, Fluid / Q000379:methods
D004272:DNA, Mitochondrial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; D012722:Sewage; D003576:Electron Transport Complex IV