title: The requirement of the DEAD-box protein DDX24 for the packaging of human immunodeficiency virus type 1 RNA.
abstract: RNA helicases play important roles in RNA metabolism. Human immunodeficiency virus type 1 (HIV-1) does not carry its own RNA helicase, the virus thus needs to exploit cellular RNA helicases to promote the replication of its RNA at various steps such as transcription, folding and transport. In this study, we report that knockdown of a DEAD-box protein named DDX24 inhibits the packaging of HIV-1 RNA and thus diminishes viral infectivity. The decreased viral RNA packaging as a result of DDX24-knockdown is observed only in the context of the Rev/RRE (Rev response element)-dependent but not the CTE (constitutive transport element)-mediated nuclear export of viral RNA, which is explained by the specific interaction of DDX24 with the Rev protein. We propose that DDX24 acts at the early phase of HIV-1 RNA metabolism prior to nuclear export and the consequence of this action extends to the viral RNA packaging stage during virus assembly.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Liwei
authors.initials: L
authors.lastname: Rong
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Bibhuti Bushan
authors.initials: BB
authors.lastname: Roy
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lu
authors.forename: Levon
authors.initials: L
authors.lastname: Abrahamyan
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Mouland
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Pan
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
mesh_terms: D001483:Base Sequence; D053487:DEAD-box RNA Helicases / Q000235:genetics* / Q000378:metabolism*; D020868:Gene Silencing; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000378:metabolism; D019065:Virus Assembly / Q000502:physiology*
chemical_list: D016655:HIV Core Protein p24; D012367:RNA, Viral; C412870:DDX24 protein, human; D053487:DEAD-box RNA Helicases