Transformation and gene replacement in the facultatively chemoheterotrophic, unicellular cyanobacterium Synechocystis sp. PCC6714 by electroporation.
The unicellular cyanobacterium Synechocystis sp. PCC6714 can grow not only under photoautotrophic conditions, but also under chemoheterotrophic conditions if glucose is added to the medium. This makes it useful for the study of many aspects of bioenergetic mechanisms. In contrast to its closely related strain Synechocystis sp. PCC6803, which cannot grow chemoheterotrophically, Synechocystis PCC6714 is not naturally transformable. To enable gene transfer in this strain, we established a method for the introduction of self-replicating IncQ plasmids and for gene replacement using electroporation.
Ludwig
Alfred
A
Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinärplatz 1, Vienna 1210, Austria. gke-vcr@hotmail.com
Heimbucher
Thomas
T
Gregor
Wolfgang
W
Czerny
Thomas
T
Schmetterer
Georg
G
D052837:Chemoautotrophic Growth; D000458:Cyanobacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial