Prevalence of virulence genes and cytolethal distending toxin production in Campylobacter jejuni isolates from diarrheal patients in Bangladesh.
From 300 stool samples, 58 Campylobacter strains were isolated by standard microbiological and biochemical methods. Of these, 40 strains were identified as Campylobacter jejuni and 5 as Campylobacter coli. The presence of flaA (100%), cadF (100%), racR (100%), dnaJ (100%), pldA (100%), ciaB (95%), virB11 (0%), ceuE (82.5%), cdtA (97.5%), cdtB (97.5%), cdtC (97.5%), and wlaN (7.5%) genes was detected in C. jejuni by PCR. All C. jejuni strains but one produced cytolethal distending toxin in a HeLa cell assay.
Talukder
Kaisar A
KA
Enteric Microbiology Laboratory, Laboratory Sciences Division, ICDDR,B, GPO Box 128, Dhaka 1000, Bangladesh. kaisar@icddrb.org
Aslam
Mohammad
M
Islam
Zhahirul
Z
Azmi
Ishrat J
IJ
Dutta
Dilip K
DK
Hossain
Sabir
S
Nur-E-Kamal
Alam
A
Nair
Gopinath B
GB
Cravioto
Alejandro
A
Sack
David A
DA
Endtz
Hubert P
HP
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics / Q000633:toxicity; D001459:Bangladesh / Q000453:epidemiology; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D037521:Virulence Factors; C067044:cytolethal distending toxin