[The influence of hyperglycemia on oxidation-reduction reaction of human umbilical vein endothelial cells].
Hyperglicemia is recognised as a primal factor responsible for microcirculatory changes in diabetic patients. There exist many reasons to claim that high glucose concentrations, generating reactive oxygen species (ROS), induce oxidative stress which initiates peroxidation processes. The end products of lipids peroxidation (aldehydes) cause disturbances of cellular membrane structures, its depolarization, inhibition of membrane's enzymes activity, indicating loss of cellular membrane integrity and uncoupling of oxidative phosphorylation in mitochondria. AIM OF THIS STUDY was to assess the influence of hyperglycemia on oxidation-reduction reaction of human umbilical vein endothelial cells, by determining malonyldialdehyde (MDA) content, a lipids peroxidation marker.


METHODS
Human umbilical vein endothelial cells were cultured in a standard medium (M 199). The experiment was performed five times on forth passage of HUVEC culture. The cultures were incubated for 48 hours with glucose in concentrations 5.5; 11.1 and 22.2 mmol/l. In the HUVEC lysate malonyldialdehyde (MDA) concentration was measured by HPLC method.


RESULTS
It was proved that MDA content in the control culture cells (5.5 mmol/l of glucose) was 15.1 +/- 1.6 nmol/mg protein. In the conditions of hyperglycemia (11.1 and 22.2 mmol/l) MDA content increased (respectively: 18.1 +/- 1.3 and 21.5 +/- 2.5 nmol/mg protein) and the differences were statistically significant (p<0.05).


CONCLUSIONS
Hyperglycemia disturbs normal oxidation-reduction reaction of human umbilical vein endothelial cells inducing oxidative stress.
Szczepański
Marek
M
Kliniki Neonatologii Akademii Medycznej w Białymstoku. szczepanski5@gazeta.pl
Kamianowska
Monika
M
Skrzydlewska
Elzbieta
E
D001786:Blood Glucose; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D010084:Oxidation-Reduction; D014471:Umbilical Veins / Q000378:metabolism*
D001786:Blood Glucose; D008315:Malondialdehyde