Mitotic, but not meiotic, oriented cell divisions in rat spermatogenesis.
The process of mammalian spermatogenesis involves both mitosis and meiosis at the same developmental age. Most previous studies have focused on mitotic spindle orientation during development, but not during meiotic division. Therefore, we asked whether there is a difference between mitotic and meiotic germ cell spindle orientation during rat spermatogenesis. Our results showed that mitotic spindles of spermatogonia were mainly oriented with angles ranging from 60 to 90 degrees, perpendicular in relation to the basement membrane of the seminiferous tubules. On the other hand, meiotic spindles showed a random orientation. Nocodazole treatment (at a concentration that depolymerizes only astral microtubules) induced a significant increase in cells with an angle between 0 and 30 degrees (parallel) in relation to the basement membrane. Meiotic spindles did not show a significant change in their orientation after the Nocodazole treatment. Therefore, our results suggest differences between the mechanisms controlling positioning and orientation of mitotic and meiotic spindles during rat spermatogenesis. It seems that a phylogenetically conserved programme controls the mitotic spindle orientation in organisms ranging from worms to mammals.
Lagos-Cabré
Raul
R
Departamento de Ciencias Fisiológicas, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, 8331010 Santiago, Chile.
Moreno
Ricardo D
RD
D000818:Animals; D007267:Injections; D008297:Male; D008540:Meiosis; D008938:Mitosis; D015739:Nocodazole / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*; D008941:Spindle Apparatus / Q000187:drug effects / Q000648:ultrastructure*; D050257:Tubulin Modulators / Q000494:pharmacology
D050257:Tubulin Modulators; D015739:Nocodazole