Synthesis of cell-penetrating peptides and their application in neurobiology.
Short basic amino acid sequences, often called cell-penetrating peptides (CPPs), allow the delivery of proteins and other molecules into cells and across the blood-brain barrier (BBB). Although the ability of basic proteins to facilitate such trafficking is known for a long time, only the application of genetic methods and overexpression of fusion proteins in Escherichia coli has lead to a wide application of CPP in many research areas, including signal transduction, cancer, angiogenesis, apoptosis, bone development, cardioprotection, cell cycle, neurobiology, and many others. For the neuroscientist, CPPs are particularly attractive, as a number of articles in the last 5 years have reported their use for neuronal rescue in a number of models for neurodegenerative diseases in vitro and in vivo in rats, mice, or gerbils. Here, we give a detailed description of the protein purification methodology and applications in neuroscience.
Dietz
Gunnar P H
GP
Neurologische Universitätsklinik, Göttingen, Germany.
Bähr
Mathias
M
D000595:Amino Acid Sequence / Q000235:genetics; D024361:Amino Acids, Basic / Q000235:genetics / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D001812:Blood-Brain Barrier; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics; D005849:Gerbillinae; D006801:Humans; D051379:Mice; D019636:Neurodegenerative Diseases / Q000188:drug therapy* / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000378:metabolism* / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000627:therapeutic use
D024361:Amino Acids, Basic; D010455:Peptides; D011993:Recombinant Fusion Proteins