[Cytokines mechanism of shenfu injection in treatment of cardiogenic shock in canine].
OBJECTIVE
To investigate the effect of shenfu injection on canine with cardiogenic shock and the possible mechanism.


METHODS
Cardiogenic shock model of canine was established by ligating left anterior descending (LAD) of coronary artery. The 15 canines with cardiogenic shock were randomly divided in to glucose injection group, shenfu injection group and sham-operated group. The hemodynamics parameters were monitored. Plasma TNF-alpha and IL-1beta levels were measured by radioimmunoassay. Expression of TNF-alpha mRNA and IL-1beta mRNA in myocardium were detected by RT-PCR.


RESULTS
Following cardiogenic shock, the mean artery pressure (MAP), left ventricular systolic pressure (LVSP), ventricular pressure rise ratio during systolic period (+ dp/dt(max)), and ventricular pressure decay ratio during diastolic period (- dp/dt(max)) decreased significantly; the plasma TNF-alpha and IL-1beta levels and the expression of TNF-a mRNA and IL-1beta mRNA in myocardium increased significantly. In shenfu injection group, MAP, LVSP and +/- dp/dt(max) increased significantly and plasma TNF-alpha and IL-1beta levels decreased significantly. In glucose injection group, MAP, LVSP, +/- dp/dt(max) and plasma TNF-alpha and IL-1beta levels had not changed significantly. The expression of TNF-alpha mRNA and IL-1beta mRNA in myocardium were significantly lower in shenfu injection group than those in glucose injection group.


CONCLUSIONS
Shenfu injection probably can decrease over-exprssion of TNF-alpha mRNA and IL-1beta mRNA on transcription platform. Shenfu injection counteract cardiogenic shock, relieve myocardium damage and improve hemodynamics through inhibiting overproduction of TNF-alpha and IL-1beta.
Ke
Da-Zhi
DZ
Department of Geriatrics, The Second Affiliated Hospital of Chongqing University of Medical Science, Chongqing 400010, China.
Chen
Qing-Wei
QW
Li
Chun-Li
CL
Li
Gui-Qiong
GQ
D031841:Aconitum / Q000737:chemistry; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D004285:Dogs; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D007275:Injections, Intravenous; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D008297:Male; D009206:Myocardium / Q000378:metabolism; D005894:Panax / Q000737:chemistry; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012770:Shock, Cardiogenic / Q000503:physiopathology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000097:blood / Q000235:genetics
D016207:Cytokines; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D012333:RNA, Messenger; C492793:Shen-Fu; D014409:Tumor Necrosis Factor-alpha