Renin inhibition activity by chitooligosaccharides.
Six kinds of chitooligosaccharides (COSs) with different molecular weight (MW) and degree of deacetylation (DD) were prepared using ultrafiltration membrane reactor, and their renin inhibition modes were evaluated. All the COSs showed the renin-inhibitory activities with dose-dependent manner, and 90-COSs had the potent renin-inhibitory activity than that of 50-COSs. Among them, 90-MMWCOS (1000-5000Da) exhibits the highest activity with IC(50) value of 0.51mg/mL and acts as competitive inhibitor with K(i) value of 0.28mg/mL by Lineweaver-Burk and Dixon plots. These results indicated that DD value and MW of COSs are important factors affecting renin-inhibitory activity.
Park
Pyo-Jam
PJ
Department of Biotechnology, Kunkuk University, Chungju 380-701, Republic of Korea.
Ahn
Chang-Bum
CB
Jeon
You-Jin
YJ
Je
Jae-Young
JY
D000107:Acetylation; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000494:pharmacology*; D000092502:Renin Inhibitors
D000806:Angiotensin-Converting Enzyme Inhibitors; D002686:Chitin; D009844:Oligosaccharides; D000092502:Renin Inhibitors