title: Host inflammatory response profiling in preeclampsia using an in vitro whole blood stimulation model.
abstract: The pathophysiology of preeclampsia (PET) implicates an inflammatory dysfunction. This study profiled this host response by challenging whole blood with lipopolysaccharide. Multiplex immunoassays determined interleukin (IL)-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12(p70), IL-13, IL-17, granulocyte/granulocyte macrophage-colony stimulating factors (G-CSF/GM-SCF), interferon(IFN)-gamma, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein-1beta and tumor necrosis factor (TNF)-alpha levels. Secretory capacity was expressed in pg/million white cells or monocytes (+/-SEM). PET featured significantly higher IL-1beta, IL-2, IL-10, IL-13, G-CSF, IFN-gamma, MCP-1 and TNF-alpha monocyte secretory capacities (p < 0.05). The PET group exhibited an inflammatory hyper-responsiveness (p < 0.01) which was poorly described by the traditional Th1:Th2 dichotomy.
authors.affiliation: Perinatal Research Group, The Liz Dawn Pathology and Translational Sciences Centre, Level 4, Leeds Institute of Molecular Medicine, JIF Building, St James's University Hospital, Leeds, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Brewster
authors.forename: N M
authors.initials: NM
authors.lastname: Orsi
authors.forename: N
authors.initials: N
authors.lastname: Gopichandran
authors.forename: U V
authors.initials: UV
authors.lastname: Ekbote
authors.forename: E
authors.initials: E
authors.lastname: Cadogan
authors.forename: J J
authors.initials: JJ
authors.lastname: Walker
mesh_terms: D000293:Adolescent; D000328:Adult; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000097:blood*; D008070:Lipopolysaccharides / Q000097:blood*; D008297:Male; D008954:Models, Biological; D011225:Pre-Eclampsia / Q000276:immunology / Q000503:physiopathology*; D011247:Pregnancy
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides