Multiplexed dendritic targeting of alpha calcium calmodulin-dependent protein kinase II, neurogranin, and activity-regulated cytoskeleton-associated protein RNAs by the A2 pathway.
In neurons, many different RNAs are targeted to dendrites where local expression of the encoded proteins mediates synaptic plasticity during learning and memory. It is not known whether each RNA follows a separate trafficking pathway or whether multiple RNAs are targeted to dendrites by the same pathway. Here, we show that RNAs encoding alpha calcium calmodulin-dependent protein kinase II, neurogranin, and activity-regulated cytoskeleton-associated protein are coassembled into the same RNA granules and targeted to dendrites by the same cis/trans-determinants (heterogeneous nuclear ribonucleoprotein [hnRNP] A2 response element and hnRNP A2) that mediate dendritic targeting of myelin basic protein RNA by the A2 pathway in oligodendrocytes. Multiplexed dendritic targeting of different RNAs by the same pathway represents a new organizing principle for coordinating gene expression at the synapse.
Gao
Yuanzheng
Y
Neuroscience Program, Department of Molecular Microbial and Structural Biology, University of Connecticut Health Center, Farmington CT 06030, USA.
Tatavarty
Vedakumar
V
Korza
George
G
Levin
Mikhail K
MK
Carson
John H
JH
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001483:Base Sequence; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D017124:Conserved Sequence; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003712:Dendrites / Q000187:drug effects / Q000201:enzymology*; D004597:Electroshock; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051579:Neurogranin / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D034443:RNA Transport / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020218:Response Elements; D013347:Subcellular Fractions / Q000187:drug effects
D000906:Antibodies; D003598:Cytoskeletal Proteins; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D009419:Nerve Tissue Proteins; C092069:activity regulated cytoskeletal-associated protein; D051579:Neurogranin; D012313:RNA; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2