title: Determination of rimantadine in rat plasma by liquid chromatography/electrospray mass spectrometry and its application in a pharmacokinetic study.
abstract: A rapid and sensitive liquid chromatography/mass spectrometry (LC/MS) method was developed and validated for the determination of rimantadine in rat plasma. Rimantadine was extracted by protein precipitation with methanol, and the chromatographic separation was performed on a C(18) column. The total analytical run time was relatively short (4.6 min), and the limit of assay quantification (LLOQ) was 2 ng/mL using 50 microL of rat plasma. Rimantadine and the internal standard (amantadine) were monitored in selected ion monitoring (SIM) mode at m/z 180.2 and 152.1, respectively. The standard curve was linear over a concentration range from 2 to 750 ng/mL, and the correlation coefficients were greater than 0.999. The mean intra- and inter-day assay accuracy ranged from 100.1-105.0% to 100.3-104.0%, respectively, and the mean intra- and inter-day precision was between 1.3-2.3% and 1.8-3.0%, respectively. The developed assay method was successfully applied to a pharmacokinetic study in rats after oral administration of rimantadine hydrochloride at the dose of 20 mg/kg.
authors.affiliation: Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China. windcolour11@hotmail.com <windcolour11@hotmail.com>
authors.forename: Meijuan
authors.initials: M
authors.lastname: Xu
authors.forename: Wenzheng
authors.initials: W
authors.lastname: Ju
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xia
authors.forename: Hengshan
authors.initials: H
authors.lastname: Tan
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ningning
authors.initials: N
authors.lastname: Xiong
authors.forename: Meng
authors.initials: M
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Lixiu
authors.initials: L
authors.lastname: Gong
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012299:Rimantadine / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000998:Antiviral Agents; D012299:Rimantadine