Aminomethylphosphonic acid accumulation in plant species treated with glyphosate.
Aminomethylphosphonic acid (AMPA) is the most frequently detected metabolite of glyphosate in plants. The objective of this study was to determine if there is any correlation of metabolism of glyphosate to AMPA in different plant species and their natural level of resistance to glyphosate. Greenhouse studies were conducted to determine the glyphosate I 50 values (rate required to cause a 50% reduction in plant growth) and to quantify AMPA and shikimate concentrations in selected leguminous and nonleguminous species treated with glyphosate at respective I 50 rates. Coffee senna [ Cassia occidentalis (L.) Link] was the most sensitive ( I 50 = 75 g/ha) and hemp sesbania [ Sesbania herbacea (P.Mill.) McVaugh] was the most resistant ( I 50 = 456 g/ha) to glyphosate. Hemp sesbania was 6-fold and Illinois bundleflower [ Desmanthus illinoensis (Michx.) MacM. ex B.L.Robins. & Fern.] was 4-fold more resistant to glyphosate than coffee senna. Glyphosate was present in all plant species, and its concentration ranged from 0.308 to 38.7 microg/g of tissue. AMPA was present in all leguminous species studied except hemp sesbania. AMPA concentration ranged from 0.119 to 4.77 microg/g of tissue. Shikimate was present in all plant species treated with glyphosate, and levels ranged from 0.053 to 16.5 mg/g of tissue. Non-glyphosate-resistant (non-GR) soybean accumulated much higher shikimate than glyphosate-resistant (GR) soybean. Although some leguminous species were found to be more resistant to glyphosate than others, and there was considerable variation between species in the glyphosate to AMPA levels found, metabolism of glyphosate to AMPA did not appear to be a common factor in explaining natural resistance levels.
Reddy
Krishna N
KN
Southern Weed Science Research Unit, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 350, Stoneville, Mississippi 38776, USA. krishna.reddy@ars.usda.gov
Rimando
Agnes M
AM
Duke
Stephen O
SO
Nandula
Vijay K
VK
D007887:Fabaceae / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology*; D007555:Isoxazoles; D063065:Organophosphonates / Q000032:analysis / Q000378:metabolism*; D010944:Plants / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D012765:Shikimic Acid / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D013777:Tetrazoles; D000097797:Glyphosate
D005998:Glycine; D006540:Herbicides; D007555:Isoxazoles; D063065:Organophosphonates; D012765:Shikimic Acid; D013777:Tetrazoles; D000097797:Glyphosate; C418233:2-amino-3-(3-hydrox-5(2-methyl-2H-tetrazol-5-yl)isoxazol-4-yl)propionic acid