The endogenous CCK mediation of electroacupuncture stimulation-induced satiety in rats.
A major satiety hormone, cholecystokinin (CCK) is well known to be released by electroacupuncture (EA) stimulation at certain body sites which elicits profound psychophysiological responses. Previous clinical and animal studies have shown that EA stimulation reduces food intake and body weight in both normal and obese subjects. The aim of the present study was to elucidate the satiety effect of EA stimulation and its mechanism related to CCK in rats. Here we show that EA stimulation at "Zusanli" (ST36) acupoint significantly reduced 30-min and 60-min food intake in 48-h fasted Sprague-Dawley rats, and such effect was reversed by a lorglumide (CCK-1 receptor antagonist, 10mg/kg, i.p.) pretreatment. The ST36 EA stimulation-induced satiety was not observed in CCK-1 receptor knockout, Otsuka Long-Evans Tokushima Fatty rats, but in their controls, Long-Evans Tokushima Otsuka rats. Subdiaphragmatic vagotomy also blocked the satiety effect of ST36 EA stimulation in Sprague-Dawley rats. These results suggest that ST36 EA stimulation elicits satiety in rats and this is mediated by the endogenous CCK signaling pathway.
Kim
Sun Kwang
SK
Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul 130-701, Republic of Korea.
Bae
Hyunsu
H
Lee
Giseog
G
Jeong
Hyunjeong
H
Woo
Hyun Su
HS
Han
Jae-Bok
JB
Kim
Yangseok
Y
Lee
Hyejung
H
Shin
Min-Kyu
MK
Hong
Moo-Chang
MC
Jin
Young-Ho
YH
Min
Byung-Il
BI
D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism*; D015671:Electroacupuncture; D005215:Fasting / Q000502:physiology; D008297:Male; D011377:Proglumide / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D020334:Rats, Inbred OLETF; D017207:Rats, Sprague-Dawley; D012527:Satiation / Q000502:physiology*
D002766:Cholecystokinin; D011377:Proglumide; C048181:lorglumide