Effect of acute lindane and alcohol intoxication on serum concentration of enzymes and fatty acids in rats.
This study examines possible synergistic effects of lindane and ethanol on inducing liver injury and serum fatty acid derangement in adult male Wistar rats. When administered together, ethanol and lindane-induced even more pronounced increase of alanine aminotransferase (165 +/- 10 U/L) and gamma-glutamyltranspeptidase activity (10.3 +/- 0.6 U/L) than after isolated administration of either substance. In addition, separate administration of lindane and ethanol was followed by a significant decrease of linoleic acid level in the serum (301 +/- 38 mg/L, 276 +/- 35 mg/L vs. 416 +/- 48 mg/L). However, when ethanol administration was followed by lindane injection, serum linoleic acid was at the similar level found in the control group (516 +/- 62 mg/L). Ethanol-treated rats that received lindane 30 min after ethanol administration have shown a marked increase of palmitic (421 +/- 27 mg/L) and linolic acid level (43 +/- 5 mg/L) in comparison with rats that have been treated only with ethanol (316+/-26 mg/L for palmitic and 32 +/- 2 mg/L for linolic acid) or lindane (295 +/- 26 mg/L for palmitic and 301 +/- 38 mg/L for linolic acid). Linolic acid level was significantly greater in comparison with control group (29 +/- 1 mg/L). In conclusion, this study found enough evidence to support the hypothesis that acute ethanol intoxication potentiates lindane-induced liver injury and enhances lipid derangement.
Radosavljević
T
T
Department of Pathophysiology, School of Medicine, University of Belgrade, Belgrade, Serbia. tanjamm@med.bg.ac.yu
Mladenović
D
D
Vucević
D
D
Petrović
J
J
Hrncić
D
D
Djuric
D
D
Loncar-Stevanović
H
H
Stanojlović
O
O
D000435:Alcoholic Intoxication / Q000097:blood* / Q000201:enzymology*; D000818:Animals; D002492:Central Nervous System Depressants / Q000097:blood; D000431:Ethanol / Q000097:blood; D005227:Fatty Acids / Q000097:blood*; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D005227:Fatty Acids; D007306:Insecticides; D000431:Ethanol; D001556:Hexachlorocyclohexane