title: Development of novel magnetic nano-carriers for high-performance affinity purification.
abstract: We developed novel magnetic nano-carriers around 180 nm in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nano-carriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nishio
authors.forename: Yuka
authors.initials: Y
authors.lastname: Masaike
authors.forename: Morihito
authors.initials: M
authors.lastname: Ikeda
authors.forename: Hiroki
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Gokon
authors.forename: Shingo
authors.initials: S
authors.lastname: Tsubouchi
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Naohiro
authors.initials: N
authors.lastname: Hanyu
authors.forename: Adarsh
authors.initials: A
authors.lastname: Sandhu
authors.forename: Haruma
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Masanori
authors.initials: M
authors.lastname: Abe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage; D002846:Chromatography, Affinity; D016503:Drug Delivery Systems; D004852:Epoxy Compounds / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D008027:Light; D008280:Magnetics; D008689:Methacrylates / Q000737:chemistry; D008727:Methotrexate / Q000008:administration & dosage; D008863:Microspheres; D008956:Models, Chemical; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D012542:Scattering, Radiation; D020058:Styrene / Q000737:chemistry; D013818:Thermogravimetry
chemical_list: D000964:Antimetabolites, Antineoplastic; D004852:Epoxy Compounds; D005290:Ferric Compounds; D008689:Methacrylates; D011108:Polymers; C001215:ferrite; D020058:Styrene; C007870:glycidyl methacrylate; D008727:Methotrexate