Oxidase-peroxidase reaction: kinetics of peroxidase-catalysed oxidation of 2-aminophenol.
Possible reaction pathways that may lead to horseradish peroxidase inactivation during the aerobic oxidation of 2-aminophenol were investigated using extended kinetic curves. A kinetic model involving the formation of a low-reactive species, Compound III, was proposed and several rate constants were calculated using an optimisation computing program. Sensitivity analysis allowed to conclude that both oxidase and peroxidase cycles occur in 2-aminophenol oxidation.
Puiu
Mihaela
M
Department of Physical Chemistry, University of Bucharest, Bucharest, Romania. mpuiu@gw-chimie.math.unibuc.ro
Răducan
Adina
A
Babaligea
Irina
I
Oancea
Dumitru
D
D000627:Aminophenols / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods; D002384:Catalysis; D003198:Computer Simulation; D006735:Horseradish Peroxidase / Q000737:chemistry; D007700:Kinetics; D008345:Manganese / Q000737:chemistry; D008956:Models, Chemical; D010088:Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D013997:Time Factors
D000627:Aminophenols; C027667:2-aminophenol; D008345:Manganese; D010088:Oxidoreductases; D006735:Horseradish Peroxidase; D009195:Peroxidase; D010100:Oxygen