title: Microscale HPLC enables a new paradigm for commercialization of complex chiral stationary phases.
abstract: The small column size (0.3 mm i.d. x 15 cm) used in microscale HPLC contains only a small fraction (<1%) of the chromatographic packing material of a typical analytical HPLC column. Consequently, chromatographic stationary phases that are prohibitively expensive in conventional HPLC, owing either to synthetic complexity or costly starting materials, may become commercially viable in the microscale format. To illustrate this point, a previously described, synthetically complex, crown ether chiral stationary phase was prepared and evaluated in the microscale format, showing excellent separation of the enantiomers of underivatized amine analytes.
authors.affiliation: Separation and Purification Center of Excellence, Merck & Co, Inc., Rahway, New Jersey 07065, USA. christopher_welch@merck.com
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Welch
authors.forename: Myung Ho
authors.initials: MH
authors.lastname: Hyun
authors.forename: Takateru
authors.initials: T
authors.lastname: Kubota
authors.forename: Wes
authors.initials: W
authors.lastname: Schafer
authors.forename: Frank
authors.initials: F
authors.lastname: Bernardoni
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Choi
authors.forename: Naijun
authors.initials: N
authors.lastname: Wu
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Gong
authors.forename: Bruce
authors.initials: B
authors.lastname: Lipshutz
mesh_terms: D000588:Amines / Q000737:chemistry / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D043844:Crown Ethers / Q000138:chemical synthesis / Q000737:chemistry; D007202:Indicators and Reagents; D008832:Microchemistry / Q000379:methods; D013237:Stereoisomerism
chemical_list: D000588:Amines; D043844:Crown Ethers; D007202:Indicators and Reagents