Methodologies for staining and visualisation of beta-galactosidase in mouse embryos and tissues.
This chapter provides information on beta-galactosidase staining of whole mouse embryos, organs, tissue sections, and cultured cells, as well as double staining with horseradish peroxidase and use as a tool for genotyping. Using these protocols, localization of beta-galactosidase can be visualized throughout development and in adult tissues. beta-Galactosidase staining may be used purely as a marker of gene expression and also as a tracer in cell lineage studies.
Loughna
Siobhan
S
School of Biomedical Sciences, University of Nottingham, UK.
Henderson
Deborah
D
D000818:Animals; D004396:Coloring Agents / Q000737:chemistry; D004622:Embryo, Mammalian / Q000737:chemistry*; D017930:Genes, Reporter; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D013194:Staining and Labeling / Q000379:methods*; D001616:beta-Galactosidase / Q000032:analysis* / Q000235:genetics
D004396:Coloring Agents; D001616:beta-Galactosidase