Construction of recombinant mouse IgG1 antibody directed against varicella zoster virus immediate early protein 63.
Five varicella zoster virus (VZV) genes are known to be transcribed in latently infected human ganglia. Transcripts from VZV gene 63, which encodes an immediate early (IE) protein, are the most prevalent and abundant. To obtain a reagent that might facilitate studies of the role of the IE63 protein in latency and reactivation, we selected an IE63-specific Fab fragment from a phage library and used it to prepare a recombinant mouse IgG1 antibody that detects IE63 and functions in Western blot, immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence assays.
Mueller
Niklaus H
NH
Department of Neurology, University of Colorado School of Medicine, Denver, Colorado 80262, USA.
Graf
Laurie L
LL
Shearer
Andrew J
AJ
Owens
Gregory P
GP
Gilden
Donald H
DH
Cohrs
Randall J
RJ
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002644:Chickenpox / Q000276:immunology / Q000821:virology; D005814:Genes, Viral / Q000276:immunology*; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D019151:Peptide Library; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
D000956:Antigens, Viral; D007074:Immunoglobulin G; D019151:Peptide Library; D011994:Recombinant Proteins