Enhanced photoelectrochemical method for linear DNA hybridization detection using Au-nanopaticle labeled DNA as probe onto titanium dioxide electrode.
A photoelectrochemical method was proposed to detect DNA hybridization using Au nanoparticle modified DNA as one probe on TiO2 substrate, in which the TiO2 substrate was used not only as DNA anchors but also as the signal transducers. Hybridization between the probe and the target DNA oligonucleotides was confirmed by the decreased photocurrent of the TiO2 electrode. Compared with non-label probe, Au nanoparticles enhanced the photocurrent shifts after the hybridization. The photocurrent decreased with increasing the concentration of target DNA, indicating that this method could be used for quantitative measurements, and the discrimination of the complementary from mismatched DNA. Furthermore, the hybridization binding constant was obtained and photocurrent generation mechanism was discussed. The major advantages of this photochemical method are speed, simplicity and excellent specificity. This method provides a platform for studying a wide variety of biological processes using photoelectrochemical method.
Lu
Wu
W
Department of Chemistry, Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.
Jin
Yan
Y
Wang
Geng
G
Chen
Da
D
Li
Jinghong
J
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000235:genetics*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D017403:In Situ Hybridization / Q000379:methods*; D008839:Microelectrodes; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D010777:Photochemistry / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*
D015342:DNA Probes; D006046:Gold