title: Preparation of a Tat-related transporter peptide for carrying the adenovirus vector into cells.
abstract: We synthesized a Tat-related peptide acetyl-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly-Cys amide, Ac-Tat(48-60)-Gly-Cys-NH(2), having high intracellular permeability, and conjugated this peptide to adenovirus vector to enhance gene transfer efficiency of adenovirus vector into cells. The peptide was prepared by the solid-phase peptide synthesis method and a bifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide ester was used to conjugate the peptide to adenovirus vector containing luciferase gene. The novel conjugate of adenovirus vector and Ac-Tat(48-60)-Gly-Cys-NH(2) peptide exhibited excellent gene transfer efficacy in B16BL6 cells.
authors.affiliation: Kobe Gakuin University, Minatojima, Chuo-ku, Kobe 650-8586, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kida
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Eto
authors.forename: Mitsuko
authors.initials: M
authors.lastname: Maeda
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Keiko
authors.initials: K
authors.lastname: Hojo
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Tadanori
authors.initials: T
authors.lastname: Mayumia
authors.forename: Koichi
authors.initials: K
authors.lastname: Kawasaki
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D015696:Gene Products, tat; D010455:Peptides