title: Structural analysis of the alpha-D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme.
abstract: The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1-->3,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows: [Formula: see text].
authors.affiliation: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Sander S
authors.initials: SS
authors.lastname: van Leeuwen
authors.forename: Slavko
authors.initials: S
authors.lastname: Kralj
authors.forename: Ineke H
authors.initials: IH
authors.lastname: van Geel-Schutten
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Gerwig
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
authors.forename: Johannis P
authors.initials: JP
authors.lastname: Kamerling
mesh_terms: D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D052200:Limosilactobacillus reuteri / Q000737:chemistry / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005936:Glucans; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; C009288:metaperiodate; D016695:Glycosyltransferases; C033837:alternansucrase