title: Evaluation of human mesenchymal stem cells response to biomimetic bioglass-collagen-hyaluronic acid-phosphatidylserine composite scaffolds for bone tissue engineering.
abstract: The aim of this study was to examine in vitro the response of human mesenchymal stem cells (hMSCs) on the novel biomimetic bioglass-collagen-hyaluronic acid-phosphatidylserine (BG-COL-HYA-PS) composite scaffold for potential use in bone tissue engineering. The initial attachment, the proliferation, migration and differentiation behavior of the cells on the BG-COL-HYA-PS composites were assessed in comparison with those on pure 58sBG, BG-COL, and BG-COL-HYA composites in either growth medium (L-DMEM supplemented with 10% fetal bovine serum) or osteogenic medium (growth medium supplemented with 0.1 microM dexamethasone, 10 mM beta-glycerophosphate, and 50 microM ascorbic acid). HMSCs attached, and subsequently proliferated and migrated on the BG-COL-HYA-PS composites to a significantly higher degree. The alkaline phosphatase (ALP) staining, ALP activity and the expression of the bone associated gene ALP, osteocalcin (OC), and osteopontin (OPN) was also significantly higher in the hMSCs on the BG-COL-HYA-PS scaffolds than those on the BG-COL, BG-COL-HYA composites and the pure 58sBG. These findings suggest that the BG-COL-HYA-PS composite porous scaffolds have high potential for use as scaffolds in bone tissue engineering and repair.
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, SunYat-sen University, Guangzhou, Guangdong, China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Xu
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinbing
authors.initials: X
authors.lastname: Yu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Li
authors.forename: Xuhui
authors.initials: X
authors.lastname: Yang
authors.forename: Shunong
authors.initials: S
authors.lastname: Li
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.forename: Andy Peng
authors.initials: AP
authors.lastname: Xiang
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis*; D001853:Bone Marrow; D001842:Bone and Bones / Q000166:cytology*; D002516:Ceramics; D006801:Humans; D006820:Hyaluronic Acid; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010718:Phosphatidylserines; D023822:Tissue Engineering / Q000379:methods*
chemical_list: C022107:Bioglass; D010718:Phosphatidylserines; D006820:Hyaluronic Acid