Structural analysis of the alpha-D-glucan (EPS35-5) produced by the Lactobacillus reuteri strain 35-5 glucansucrase GTFA enzyme.
The neutral exopolysaccharide EPS35-5 (reuteran) produced from sucrose by the glucansucrase GTFA enzyme from Lactobacillus reuteri 35-5 was found to be a (1-->4,1-->6)-alpha-D-glucan, with no repeating units present. Based on linkage analysis and 1D/2D 1H and 13C NMR spectroscopy of intact EPS35-5, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis and enzymatic hydrolysis, using pullulanase M1 (Klebsiella planticola), of EPS35-5, a composite model, that includes all identified structural elements, was formulated as follows: [Formula: see text].
van Leeuwen
Sander S
SS
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Kralj
Slavko
S
van Geel-Schutten
Ineke H
IH
Gerwig
Gerrit J
GJ
Dijkhuizen
Lubbert
L
Kamerling
Johannis P
JP
D002240:Carbohydrate Sequence; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000378:metabolism; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D007709:Klebsiella / Q000201:enzymology; D052200:Limosilactobacillus reuteri / Q000737:chemistry / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism*
D005936:Glucans; D013395:Sucrose; D016695:Glycosyltransferases; C033837:alternansucrase; D006026:Glycoside Hydrolases; C100181:pullulanase