1-Aminocyclopropane-1-carboxylate deaminase enhances Agrobacterium tumefaciens-mediated gene transfer into plant cells.
Agrobacterium-mediated gene transfer is widely used for plant molecular genetics, and efficient techniques are required. Recent studies show that ethylene inhibits the gene transfer. To suppress ethylene evolution, we introduced 1-aminocyclopropane-1-carboxylate (ACC) deaminase into Agrobacterium tumefaciens. The ACC deaminase enhanced A. tumefaciens-mediated gene transfer into plants.
Nonaka
Satoko
S
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8572, Japan. ezura@gene.tsukuba.ac.jp.
Sugawara
Masayuki
M
Minamisawa
Kiwamu
K
Yuhashi
Ken-Ichi
K
Ezura
Hiroshi
H
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics*; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D018014:Gene Transfer Techniques; D022761:Gene Transfer, Horizontal
D004269:DNA, Bacterial; D005030:Ethylenes; C036216:ethylene; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases