title: Progress in developing polymerized crystalline colloidal array sensors for point-of-care detection of myocardial ischemia.
abstract: The difficulty of rapid, definitive diagnosis of myocardial ischemia leads to unnecessary hospital admissions and treatment delays. Previously, decreased metal binding affinity in human serum was investigated as a marker for myocardial ischemia. Polymerized Crystalline Colloidal Array (PCCA) sensors for Ni2+ may be useful in developing a point-of-care test to determine metal binding affinity in plasma and to help rule out myocardial ischemia. PCCA sensors for Ni2+, with 5-amino-8-hydroxyquinoline as a chelating agent, were tested in aqueous solutions and diluted human plasma. The peak wavelength diffracted by the sensors was monitored by reflectance spectrometry and correlated with Ni2+ concentration. The PCCA sensors show a linear response to aqueous Ni2+ concentrations between 0.2 and 1.0 mmol L(-1), and can detect changes in free Ni2+ concentration of <60 micromol L(-1). The sensors respond at physiologic pH and can be reversibly dehydrated. The PCCA sensors developed here can report on free Ni2+ concentration in the presence of human plasma. These sensors can be used to detect a decrease in the Ni2+ affinity of plasma proteins, which may indicate recent myocardial ischemia. PCCA sensors offer a practical approach to rapid, point-of-care detection of a proposed biochemical signature of myocardial ischemia.
authors.affiliation: Department of Chemistry, Univeristy of Pittsburgh, 219 Parkman Ave, Pittsburgh, PA 15260, USA.
authors.forename: Justin T
authors.initials: JT
authors.lastname: Baca
authors.forename: David N
authors.initials: DN
authors.lastname: Finegold
authors.forename: Sanford A
authors.initials: SA
authors.lastname: Asher
mesh_terms: D015415:Biomarkers / Q000097:blood; D003102:Colloids; D003460:Crystallization; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017202:Myocardial Ischemia / Q000175:diagnosis*; D009532:Nickel / Q000097:blood*; D019095:Point-of-Care Systems
chemical_list: D015415:Biomarkers; D003102:Colloids; D009532:Nickel