title: [Reaction of epithelial cell rests of malassez to tooth emergence and occlusal function reaction of epithelial cell rests of Malassez to tooth emergence and occlusal function].
abstract: OBJECTIVE To observe the morphology and proliferation of epithelial cell rests of Malassez (ECRM) during tooth emergence and occlusal function, and to evaluate its roles.
  
  
  METHODS
  Cytokeratin 14 (CK14) was applied as special marker of ECRM cells. The morphology and distribution of ECRM were examined by light and transmission electron microscopy. PV two-step immunohistochemical method was used to detect the expression of CK14 and proliferating cell nuclear antigen (PCNA) in ECRM.
  
  
  RESULTS
  ECRM experienced instinct morphological changes during tooth emergence and occlusal function. They were observed as network of epithelial cells labeled by CK14, especially in furcation level regions of mouse molars and active cell proliferation during occlusion found period. Cell apoptosis was observed in many ECRM by transmission electron microscopy during late stage of the progess.
  
  
  CONCLUSIONS
  ECRM may not only an accidental left-over of early embryonic development but rather play significant roles in occlusion found period.
authors.affiliation: Dept of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
authors.forename: Xi-Jiao
authors.initials: XJ
authors.lastname: Yu
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.forename: Chang-Jie
authors.initials: CJ
authors.lastname: Xiao
authors.forename: Zong-Xia
authors.initials: ZX
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D004847:Epithelial Cells; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008963:Molar; D010513:Periodontal Ligament; D012146:Rest; D014070:Tooth