title: Molecular testing for somatic cancer mutations: a survey of current and future testing in UK laboratories.
abstract: BACKGROUND
  Genetics clinical practice has paid limited attention to non-inherited aspects of cancer, namely mutations occurring during carcinogenesis. These somatic mutations are likely to be the primary determinants of cancer behaviour and treatment response, with a recent example being Her2/Neu gene status and response to Herceptin in breast cancer.
  
  
  OBJECTIVE
  To assess the feasibility of widespread testing of tumours by surveying UK histopathology and genetics laboratories.
  
  
  METHODS
  The questionnaire asked: which of the common cancers or other malignancies are routinely assessed; which molecular and cytogenetic methods are used; who orders and funds testing; what is the future demand for somatic testing; and what are the barriers to widespread testing?
  
  
  RESULTS
  Of 50 laboratories surveyed, 33 responded, 22 of which are currently using molecular tests. The survey shows that the most common tests are immunohistochemistry, fluorescence in-situ hybridisation and DNA testing of somatic mutations. Most laboratories predict testing will increase over the next 10 years, particularly for DNA testing using microarrays. Respondents perceived the main barriers to expanding molecular testing were a lack of laboratory funding and scientific evidence and testing not considered an NHS priority.
  
  
  CONCLUSIONS
  These results provide important information for healthcare commissioners faced with managing demand for molecular testing of cancers.
authors.affiliation: Health Economics Research Centre, Department of Public Health, University of Oxford, Oxford, UK. sarah.wordsworth@dphpc.ox.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Wordsworth
authors.forename: I
authors.initials: I
authors.lastname: Papanicolas
authors.forename: J
authors.initials: J
authors.lastname: Buchanan
authors.forename: I
authors.initials: I
authors.lastname: Frayling
authors.forename: J
authors.initials: J
authors.lastname: Taylor
authors.forename: I
authors.initials: I
authors.lastname: Tomlinson
mesh_terms: D003582:Cytogenetics; D003625:Data Collection; D005544:Forecasting; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D006801:Humans; D007150:Immunohistochemistry / Q000706:statistics & numerical data; D017404:In Situ Hybridization, Fluorescence / Q000706:statistics & numerical data; D007753:Laboratories; D009154:Mutation; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data; D012109:Research Support as Topic; D006113:United Kingdom