[Screening test for drugs in health foods using 2-dimensional TLC].
A simple and rapid method for screening of drugs in health foods using 2-dimensional TLC was established. The development systems were: (1) ethyl acetate/methanol/28% ammonia (85:10:5), (2) cyclohexane/toluene/diethyl amine (65:25:10), (3) methanol and (4) ethyl acetate/methanol/25% ammonia (85:10:5). System (1) and (2) were run as 2-dimensional high-performance TLC, and System (3) and (4) were run as 2-dimensional conventional TLC. Sample extracted with methanol from health foods was applied to standardized two 2-dimensional TLCs and 4 Rf values obtained from 4 development systems were compared with reference Rf values which were measured previously using standard materials. The repeatability of reference Rf values were secured by standardizing development conditions and pattern of 4 Rf values was specific for 80 drugs. Moreover, using 2-dimensional TLC enable to assign 4 Rf values to unknown substance without influence of matrix from health foods, so it was possible to determine drugs comparing 4 Rf values. This method could be applied as first screening test for prevention of health crisis occurred by drugs added to health foods illegally.
Kurita
Hiroyuki
H
Department of Drug and Food Science, Shizuoka Institute of Environment and Hygiene, Japan. kani@pref.shizuoka.lg.jp
Mizuno
Kumiko
K
Kuromi
Koichi
K
Ueno
Chie
C
Kamimura
Mitsuko
M
Fujiwara
Atsuko
A
Owada
Kazuhiro
K
Yamamoto
Masatoshi
M
D002855:Chromatography, Thin Layer / Q000379:methods* / Q000592:standards; D064420:Drug-Related Side Effects and Adverse Reactions; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D058870:Food, Organic / Q000009:adverse effects / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*
D004364:Pharmaceutical Preparations