title: Post-exposure serological and bacteriological responses of water buffalo (Bubalus bubalis) to Brucella abortus biovar 1 following vaccination with Brucella abortus strain RB51.
abstract: Serological and bacteriological responses to Brucella abortus biovar 1 following vaccination with B. abortus strain RB51 (RB51) were evaluated in thirty domestic water buffalo (Bubalus bubalis) randomly divided into five treatment groups. Groups I to V received, respectively, the recommended dose (RD) of RB51 vaccine once, RD twice 4 weeks apart, double RD once, double RD twice 4 weeks apart, and saline once (control). Vaccination did not result in a serological response. Experimental animals released 27 weeks post initial inoculation (27 PIIW) into a brucellosis-positive herd failed to seroconvert after 29 weeks. Experimental challenge commenced at 57 PIIW. All animals received B. abortus biovar 1 intraconjunctivally at 0, 5 and 9 weeks post experimental exposure (PEEW). Serum samples collected at 4, 8 and 13 PEEW were negative. At 16 PEEW all animals received B. abortus biovar 1 subcutaneously (SC), and all seroconverted by 20 PEEW. Five of twenty-six animals were positive for Brucella infection on bacterial culture. Brucella abortus biovar 1 was isolated from three animals; B. abortus RB51 was isolated from two. Treatment group, age and sex had no effect on the isolation of Brucellae (P>0.05).
authors.affiliation: Faculty of Medical Sciences, School of Veterinary Medicine, University of the West indies, Champs Fleurs, Trinidad and Tobago.
authors.forename: M D
authors.initials: MD
authors.lastname: Diptee
authors.forename: Z
authors.initials: Z
authors.lastname: Asgarali
authors.forename: M
authors.initials: M
authors.lastname: Campbell
authors.forename: G
authors.initials: G
authors.lastname: Fosgate
authors.forename: A A
authors.initials: AA
authors.lastname: Adesiyun
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002004:Brucella Vaccine / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D002020:Buffaloes / Q000097:blood / Q000276:immunology* / Q000382:microbiology*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D015151:Immunoblotting / Q000662:veterinary; D008297:Male; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine