title: Knockdown of damage-specific DNA binding protein 1 (DDB1) enhances the HBx-siRNA-mediated inhibition of HBV replication.
abstract: Recent studies have demonstrated that the effect of inhibition of HBV replication can be achieved by RNA interference (RNAi) at both the cellular and organismal levels. However, HBV replication cannot be completely inhibited by this method. To completely inhibit HBV replication, new strategies for improving the inhibition efficacy of HBV-specific siRNAs are needed. In this study, we demonstrated that knockdown of damage-specific DNA binding protein 1(DDB1), a protein involved in nucleotide-excision repair and HBV replication, significantly enhanced the HBx-siRNA-mediated inhibition of HBV replication. Although knockdown of DDB1 may be toxic to normal liver cells, our results indeed suggest a new direction to enhance the efficacy of HBV-siRNA-mediated inhibition of HBV replication.
authors.affiliation: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400010, PR China. tangkaifu@hotmail.com
authors.forename: Kai-Fu
authors.initials: KF
authors.lastname: Tang
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Xi-Yuan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Weiqun
authors.initials: W
authors.lastname: Zeng
authors.forename: Ai-Long
authors.initials: AL
authors.lastname: Huang
authors.forename: Guo-qing
authors.initials: GQ
authors.lastname: Zuo
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Xiang
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology*; D020868:Gene Silencing; D006514:Hepatitis B Surface Antigens / Q000737:chemistry; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008954:Models, Biological; D034741:RNA, Small Interfering / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
chemical_list: C091435:DDB1 protein, human; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D006514:Hepatitis B Surface Antigens; D034741:RNA, Small Interfering