title: IL-6 KO mice develop experimental amoebic liver infection with eosinophilia.
abstract: Interleukin 6 (IL-6) is a multifunctional cytokine that regulates various aspects of the immune response, such as acute phase reaction and hematopoiesis, and is an important signal that coordinates activities of liver cells, macrophages, and lymphocytes. Amoebic liver lesions have been studied, usually in hamsters, due to the problem of abscess development in mice. We report here the development of an experimental amoebic liver abscess (ALA) model in mice deficient in IL-6. Axenically grown amoebae were injected directly into the livers of C57BL/6 wild type (WT) and IL-6 KO -/- mice; the abscesses produced were counted and the inflammatory process was examined on 5, 10, and 20 days postinfection. Our results showed that IL-6 KO -/- mice develop ALA, in contrast to the WT strain, which usually do not have signs of abscess or infection. Histological analysis of the abscesses showed extended inflammatory response, mainly mediated by eosinophils, which strongly infiltrate the abscess in IL-6 K -/- mice. The present results suggest that in mice, IL-6 could play a role in the resistance against ALA.
authors.affiliation: Departamento de Patología, Instituto Nacional de Rehabilitación, Secretaría de Salud, CP 14389, México, DF.
authors.forename: Eréndira
authors.initials: E
authors.lastname: Estrada-Villaseñor
authors.forename: Jorge
authors.initials: J
authors.lastname: Morales-Montor
authors.forename: Mauricio
authors.initials: M
authors.lastname: Rodríguez-Dorantes
authors.forename: Espiridión
authors.initials: E
authors.lastname: Ramos-Martínez
authors.forename: Mario
authors.initials: M
authors.lastname: Néquiz-Avendaño
authors.forename: Pedro
authors.initials: P
authors.lastname: Ostoa-Saloma
mesh_terms: D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D004748:Entamoeba histolytica / Q000276:immunology* / Q000472:pathogenicity; D004802:Eosinophilia / Q000276:immunology* / Q000473:pathology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008101:Liver Abscess, Amebic / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D015850:Interleukin-6