Vesicle-associated membrane protein-8/endobrevin negatively regulates phagocytosis of bacteria in dendritic cells.
Phagocytosis is a specialized mechanism used by mammalian cells, particularly the cells of the immune system, such as dendritic cells (DC) and macrophages, to protect the host against infection. The process involves a complex cascade of pathways, from the ligation of surface receptors of phagocytes with components of the microorganism's surface, formation of phagosomes and subsequently phagolysosomes, to the eventual presentation of foreign Ags. Vesicle-associated membrane protein (VAMP)-8/endobrevin has been shown previously to function in the endocytic pathways. Our results showed that VAMP-8 colocalized with lysosome-associated membrane protein-2, and a significant amount of VAMP-8 was recruited to the phagosomes during bacterial ingestion. However, overexpression of VAMP-8 significantly inhibited phagocytosis in DC. We also found that the phagocytic activity of VAMP-8-/- DC was significantly higher than wild-type VAMP-8+/+ DC, thus further confirming that VAMP-8 negatively regulates phagocytosis in immature DC.
Ho
Yong Hou Sunny
YH
Laboratory of Membrane Trafficking and Immunoregulation Research, Department of Microbiology, Immunology Program, Yong Loo Lin School of Medicine, National University of Singapore, Republic of Singapore.
Cai
Deyu Tarika
DT
Wang
Cheng-Chun
CC
Huang
Dachuan
D
Wong
Siew Heng
SH
D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism* / Q000382:microbiology; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology*; D011992:Endosomes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D004926:Escherichia coli / Q000276:immunology* / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000276:immunology / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010587:Phagocytosis / Q000235:genetics / Q000276:immunology*; D050683:R-SNARE Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
D050683:R-SNARE Proteins; C491831:Vamp8 protein, mouse; D049452:Green Fluorescent Proteins