Establishment of a novel specific ELISA system for rat N- and C-ERC/mesothelin. Rat ERC/mesothelin in the body fluids of mice bearing mesothelioma.
Mesothelioma is a type of malignant tumor that most commonly arises from the pleural or peritoneal membrane and is usually associated with previous exposure to asbestos. In humans, ERC/mesothelin is expressed on the normal mesothelium and in some cancers such as mesothelioma or ovarian carcinoma. Recently, several enzyme-linked immunosorbent assay (ELISA) systems for ERC/mesothelin have been developed, the reported usefulness of which has been assessed and demonstrated as a diagnostic tool. However, the basic roles or physiological functions of, and relationship between, ERC/mesothelin and asbestos exposure-mediated carcinogenesis remain to be resolved. In order to elucidate the precise mechanism, animal models of mesothelioma are desperately needed. In this study, we consider the development of a novel specific ELISA system for not only rat N-ERC/mesothelin but also C-ERC/mesothelin, and the first data on the presence of rat ERC/mesothelin in the body fluids of rat malignant mesothelioma-bearing nude mice. The transplanted mice have revealed the higher concentrations of rat N-ERC/mesothelin in the blood and ascites than C-ERC/mesothelin. We hope these novel ELISA systems are useful in the rat model system to clarify the mechanism of asbestos-induced carcinogenesis and to develop new effective drugs for mesothelioma.
Hagiwara
Yoshiaki
Y
Immuno-Biological Laboratories, 5-1 Aramachi, Takasaki-Shi, Gunma 370-0831, Japan.
Hamada
Yukiko
Y
Kuwahara
Maki
M
Maeda
Masahiro
M
Segawa
Tatsuya
T
Ishikawa
Kiyoshi
K
Hino
Okio
O
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001194:Asbestos / Q000633:toxicity; D001826:Body Fluids / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018604:Epitope Mapping; D005434:Flow Cytometry; D058851:GPI-Linked Proteins; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D000090204:Mesothelin; D008654:Mesothelioma / Q000139:chemically induced / Q000175:diagnosis*; D051379:Mice; D008819:Mice, Nude; D010997:Pleural Neoplasms / Q000139:chemically induced / Q000175:diagnosis*; D051381:Rats
D000911:Antibodies, Monoclonal; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C000715528:Msln protein, mouse; C000715529:Msln protein, rat; D001194:Asbestos; D000090204:Mesothelin