Biotinylated nanodiamond: simple and efficient functionalization of detonation diamond.
We have developed a simple and efficient method for the covalent functionalization of detonation nanodiamond. After homogenization of the surface by borane reduction, the surface was modified with (3-aminopropyl)trimethoxysilane. Subsequent grafting of biotin yielded covalently biotinylated nanodiamond, which was characterized by FTIR spectroscopy, X-ray powder diffractometry, thermogravimetry, and elemental analysis. The activity was tested with horseradish peroxidase-labeled streptavidin. The surface loading of biotin was found to be 1.45 mmol g-1. The new material opens the way to covalently bonded diamond bioconjugates for labeling, drug delivery, and other applications.
Krueger
Anke
A
Otto-Diels-Institut für Organische Chemie, Christian-Albrechts-Universität zu Kiel, Otto-Hahn-Platz 3, Kiel, Germany. akrueger@oc.uni-kiel.de
Stegk
Jochen
J
Liang
Yuejiang
Y
Lu
Li
L
Jarre
Gerald
G
D019921:Biotinylation; D006112:Gravitation; D006735:Horseradish Peroxidase / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D017550:Spectroscopy, Fourier Transform Infrared; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism
D019809:Streptavidin; D006735:Horseradish Peroxidase