[Effect of low dose radiation on human [corrected] bone marrow mesenchymal stem cells by using proteomic analysis].
This study was aimed to investigate the effect of low dose radiation (LDR) on human bone marrow mesenchymal stem cells (MSCs) by using proteomic analysis. The bidirectional gel electrophoresis was used to establish the two-dimensional gel electrophoresis patterns of proteome in group of MSCs exposed to LDR and in group of sham irradiated MSCs, the matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was used to identify the differentially expressed proteins in two groups. The results showed that among the differentially expressed proteins in the two groups, the expressions of 12 proteins were up-regulated, the expressions of 12 protein were down-regulated, 3 proteins disappeared after LDR, 12 proteins had been identified by MALDI-TOF-MS. In conclusion, the identified 12 proteins, such as prolyl 4-hydroxylase, dihydropyrimidinase-like 2 variant, ARP3 (actin-related protein 3, yeast) homolog, guanine nucleotide binding protein (G protein), phosphoglycerate mutase 1 may be related to mechanism of LDR effect. The study provides some new explanation for the mechanism of low dose radiation injury.
Zhang
Yi-Qiong
YQ
Department of Hematology and Onocology, The First Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China.
Li
Wei
W
Wang
Guan-Jun
GJ
Yang
Yan
Y
Wang
Jie
J
Zhang
Xue-Min
XM
D051378:Actin-Related Protein 3 / Q000032:analysis; D001854:Bone Marrow Cells / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D019204:GTP-Binding Proteins / Q000032:analysis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000528:radiation effects*; D011393:Procollagen-Proline Dioxygenase / Q000032:analysis; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D011829:Radiation Dosage; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D051378:Actin-Related Protein 3; D011506:Proteins; D011393:Procollagen-Proline Dioxygenase; D019204:GTP-Binding Proteins