[Determination of beta-eudesmol in rhizome of Atractylodes lancea by RP-HPLC].
OBJECTIVE
To develop a RP-HPLC method for determination of beta-eudesmol in rhizome of Atractylodes lancea, and to provide valuble data for quality control of A. lancea.


METHODS
The samples were separated on an Inertsil ODS-3 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (68:32). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 200 nm. Column temperature was 25 degrees C.


RESULTS
The contents of beta-eudesmol determinated was 0.833-4.466 mg x g(-1), The linear range of beta-eudesmol was 0.048-1.200 microg (r = 0.999 9), the average recovery was 99.3%, RSD was 1.4% (n = 9).


CONCLUSIONS
The method for quantitation of beta-eudesmol in A. lancea was accurate and reliable, which can be used to evaluate the quality of rhizome of A. lancea.
Chen
Yan-Ming
YM
Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Institutes of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Chou
Gui-Xin
GX
Wang
Zheng-Tao
ZT
D031174:Atractylodes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D045787:Sesquiterpenes, Eudesmane / Q000032:analysis* / Q000592:standards
D045787:Sesquiterpenes, Eudesmane; C051082:beta-eudesmol