[Study on fingerprint spectrum of loquat leaf by HPLC].
OBJECTIVE
To establish the fingerprint spectrum of Loquat Leaf of Fujian authentic medicinal herbs by HPCE.


METHODS
The HPLC fingerprints of Loquat Leaf were obtained from instrument HP1100. The HPLC separation was performed on a Eclipse XDB-C18 (4.6 mm x 150 mm, 5 microm) analytical column diluted with methanol-l% glacial acetic acid (90:10) at the flow rate of 1.0 ml/min. The temperature of column was room temperature. The UV detection wavelength was 215 nm.


RESULTS
Detected 12 batches samples of different species Lopuat Leaf Fingerprint by the method of HPLC. Eight peaks in the chromatogram were common. All the similarity were above 0.9, the fingerprints integer feature were coincident essentially.


CONCLUSIONS
The method is convenient, quick and exact, and its reproducibility is good. It will be a scientific reference for qualitation identification and quality evaluation of Loquat Leaf.
Lin
Yu-lin
YL
Fujian Putian Institute of Traditional Chinese Medicine, Putian 351100, China. PTZYYLin@163.com
Xu
Rong-qing
RQ
Lin
Wen-jin
WJ
Lin
Li-qiang
LQ
Deng
Si-shan
SS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D031983:Eriobotrya / Q000737:chemistry* / Q000145:classification; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis; D000097245:Ursolic Acid
D004365:Drugs, Chinese Herbal; D014315:Triterpenes; D000097245:Ursolic Acid