title: Detection of Histoplasma capsulatum antigenuria by ultrafiltration of samples with false-negative results.
abstract: Patients with histoplasmosis may test falsely negative for Histoplasma capsulatum antigenuria. In some cases antigen is present at levels below the assay's detection limit, and ultrafiltration could improve sensitivity. Antigen was detected following ultrafiltration in 73.8% of falsely negative specimens versus 2% of controls. Ultrafiltration improved sensitivity with a small reduction in specificity.
authors.affiliation: MiraVista Diagnostics, 4444 Decatur Blvd., Suite 300, Indianapolis, IN 46241, USA. legan@miravistalabs.com
authors.forename: Lindsey
authors.initials: L
authors.lastname: Egan
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Connolly
authors.forename: Deanna
authors.initials: D
authors.lastname: Fuller
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Davis
authors.forename: John
authors.initials: J
authors.lastname: Witt
authors.forename: Kenneth S
authors.initials: KS
authors.lastname: Knox
authors.forename: Chadi A
authors.initials: CA
authors.lastname: Hage
authors.forename: L Joseph
authors.initials: LJ
authors.lastname: Wheat
mesh_terms: D000946:Antigens, Fungal / Q000652:urine*; D005188:False Negative Reactions; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000276:immunology / Q000652:urine* / Q000821:virology; D006801:Humans; D012680:Sensitivity and Specificity; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D000946:Antigens, Fungal