Synthesis, DNA binding and cleavage activities of the copper (II) complexes of estrogen-macrocyclic polyamine conjugates.
A series of the copper (II) complexes 5a-d of estrogen-macrocyclic polyamine conjugates were synthesized and characterized. The interactions of complexes 5a-d with DNA were studied by fluorescence spectroscopy and gel electrophoresis under physiological conditions. The results indicate that the conjugated estrogens have increased the cleavage efficiency of Cu[cyclen](2+) while the conjugates display poor binding affinities. The functional groups of D-ring of estrogens may play a key role in deciding binding and cleavage extent of the complexes to DNA.
Yang
Xin-Bin
XB
Department of Chemistry, Key Laboratory of Green Chemistry and Technology (Ministry of Education), Sichuan University, No. 29, Wangjiang Road, Chengdu, Sichuan 610064, PR China.
Feng
Jie
J
Zhang
Ji
J
Zhang
Zhong-Wei
ZW
Lin
Hong-Hui
HH
Zhou
Li-Hong
LH
Yu
Xiao-Qi
XQ
D000818:Animals; D002417:Cattle; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D047028:Macrocyclic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D011073:Polyamines / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D004967:Estrogens; D047028:Macrocyclic Compounds; D011073:Polyamines; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D003851:Deoxyribonucleases