title: Depot specific differences during adipogenesis of porcine stromal-vascular cells.
abstract: Recently a role of adipose tissue as an endocrine organ secreting factors involved in the regulation of whole-body energy homeostasis has emerged. Preadipocytes in different fat depots have distinct adipogenic potential and the metabolic activity differs between mature adipocytes of different depot origins. Here we describe the proliferation and differentiation of stromal-vascular cells derived from subcutaneous and visceral fat depots of adult pigs. We demonstrate that subcutaneous porcine preadipocytes proliferate more actively and that individual subcutaneous adipocytes have a more rapid accumulation of triacylglycerols than visceral cells. During differentiation, subcutaneous and visceral preadipocytes showed similar gene expression patterns with increased expression of adiponectin (APM1), adipocyte-specific fatty acid binding protein (FABP4), catalase (CAT), and peroxisome proliferator-activated receptor gamma 2 (PPARG2). Furthermore, initial data showing depot-originated effects on the expression of CAT, carnitine palmitoyl transferase 1B (CPT1B) and FABP4 suggest possible depot specific differences in the function and metabolism of mature porcine adipocytes.
authors.affiliation: Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Aas, Norway. johanna.samulin@umb.no
authors.forename: Johanna
authors.initials: J
authors.lastname: Samulin
authors.forename: Sigbjørn
authors.initials: S
authors.lastname: Lien
authors.forename: Eli
authors.initials: E
authors.lastname: Grindflek
authors.forename: Ingunn
authors.initials: I
authors.lastname: Berget
authors.forename: Bente
authors.initials: B
authors.lastname: Ruyter
authors.forename: Hilde
authors.initials: H
authors.lastname: Sundvold
mesh_terms: D017667:Adipocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism*; D013552:Swine; D014280:Triglycerides / Q000378:metabolism
chemical_list: D014280:Triglycerides