title: Gpx3-dependent responses against oxidative stress in Saccharomyces cerevisiae.
abstract: The yeast Saccharomyces cerevisiae has defense mechanisms identical to higher eukaryotes. It offers the potential for genome-wide experimental approaches owing to its smaller genome size and the availability of the complete sequence. It therefore represents an ideal eukaryotic model for studying cellular redox control and oxidative stress responses. S. cerevisiae Yap1 is a well-known transcription factor that is required for H2O2-dependent stress responses. Yap1 is involved in various signaling pathways in an oxidative stress response. The Gpx3 (Orp1/PHGpx3) protein is one of the factors related to these signaling pathways. It plays the role of a transducer that transfers the hydroperoxide signal to Yap1. In this study, using extensive proteomic and bioinformatics analyses, the function of the Gpx3 protein in an adaptive response against oxidative stress was investigated in wild-type, gpx3-deletion mutant, and gpx3-deletion mutant overexpressing Gpx3 protein strains. We identified 30 proteins that are related to the Gpx3- dependent oxidative stress responses and 17 proteins that are changed in a Gpx3-dependent manner regardless of oxidative stress. As expected, H2O2-responsive Gpx3-dependent proteins include a number of antioxidants related with cell rescue and defense. In addition, they contain a variety of proteins related to energy and carbohydrate metabolism, transcription, and protein fate. Based upon the experimental results, it is suggested that Gpx3-dependent stress adaptive response includes the regulation of genes related to the capacity to detoxify oxidants and repair oxidative stress-induced damages affected by Yap1 as well as metabolism and protein fate independent from Yap1.
authors.affiliation: Translational Research Center, KRIBB, Daejeon 806-333, Korea.
authors.forename: Chang Won
authors.initials: CW
authors.lastname: Kho
authors.forename: Phil Young
authors.initials: PY
authors.lastname: Lee
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Kang
authors.forename: Sayeon
authors.initials: S
authors.lastname: Cho
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Choong-Hyun
authors.initials: CH
authors.lastname: Sun
authors.forename: Gwan-Su
authors.initials: GS
authors.lastname: Yi
authors.forename: Byoung Chul
authors.initials: BC
authors.lastname: Park
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D015966:Gene Expression Regulation, Fungal; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D018384:Oxidative Stress; D040081:Protein Array Analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D017384:Sequence Deletion; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; C494438:HYR1 protein, S cerevisiae