Effects of (60)Co gamma radiation on thylakoid membrane functions in Anacystis nidulans.
In photosynthetic organisms oxidative stress is known to result in photoinactivation of photosynthetic machinery. We investigated effects of (60)Co gamma radiation, which generates oxidative stress, on thylakoid structure and function in cyanobacteria. Cells of unicellular, non-nitrogen fixing cyanobacterium Anacystis nidulans (Synechococcus sp.) showed D(10) value of 257 Gy of (60)Co gamma radiation. When measured immediately after exposure, cells irradiated with 1500 Gy (lethal dose) of (60)Co gamma radiation did not show any differences in photosynthetic functions such as CO(2) fixation, O(2) evolution and partial reactions of photosynthetic electron transport in comparison to unirradiated cells. Incubation of irradiated cells for 24h in light or dark resulted in decline in photosynthesis. The decline in photosynthesis was higher in the cells incubated in light as compared to the cells incubated in dark. Among the partial reactions of electron transport, only PSII activity declined drastically after incubation of irradiated samples. This was also supported by the analysis of membrane functions using thermoluminescence. Exposure of cyanobacteria to high doses of (60)Co gamma radiation did not affect the thylakoid membrane ultrastructure immediately after exposure as shown by electron microscopy. The level of reactive oxygen species (ROS) in irradiated cells was 20 times higher as compared to control. In irradiated cells de novo protein synthesis was reduced considerably immediately after irradiation. Treatment of cells with tetracycline also affected photosynthesis as in irradiated cells. The results showed that photoinhibition of photosynthetic apparatus after incubation of irradiated cells was probably augmented due to reduced protein synthesis. Active photosynthesis is known to require uninterrupted replenishment of some of the proteins involved in electron transport chain. The defective thylakoid membrane biogenesis may be leading to photosynthetic decline post-irradiation.
Agarwal
Rachna
R
Molecular Biology Division, Bhabha Atomic Research Center, Mumbai 400 085, India.
Rane
S S
SS
Sainis
Jayashree Krishna
JK
D002245:Carbon Dioxide / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D003037:Cobalt Radioisotopes; D005720:Gamma Rays; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D008715:Methionine / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress; D010788:Photosynthesis / Q000528:radiation effects*; D045331:Photosystem I Protein Complex / Q000378:metabolism; D045332:Photosystem II Protein Complex / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D046940:Synechococcus / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D020524:Thylakoids / Q000378:metabolism / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D002734:Chlorophyll; D002245:Carbon Dioxide; D008715:Methionine