fRMSDPred: predicting local RMSD between structural fragments using sequence information.
The effectiveness of comparative modeling approaches for protein structure prediction can be substantially improved by incorporating predicted structural information in the initial sequence-structure alignment. Motivated by the approaches used to align protein structures, this article focuses on developing machine learning approaches for estimating the RMSD value of a pair of protein fragments. These estimated fragment-level RMSD values can be used to construct the alignment, assess the quality of an alignment, and identify high-quality alignment segments. We present algorithms to solve this fragment-level RMSD prediction problem using a supervised learning framework based on support vector regression and classification that incorporates protein profiles, predicted secondary structure, effective information encoding schemes, and novel second-order pairwise exponential kernel functions. Our comprehensive empirical study shows superior results compared with the profile-to-profile scoring schemes. We also show that for protein pairs with low sequence similarity (less than 12% sequence identity) these new local structural features alone or in conjunction with profile-based information lead to alignments that are considerably accurate than those obtained by schemes that use only profile and/or predicted secondary structure information.
Rangwala
Huzefa
H
Computer Science and Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA. rangwala@cs.umn.edu
Karypis
George
G
D000465:Algorithms; D030562:Databases, Protein; D011506:Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
D011506:Proteins