Synthesis of chlorinated fluoresceins for labeling proteins.
Two novel chlorinated fluoresceins 4,7,2',7'-tetrachloro-6-(5-carboxypentyl)fluorescein (8a) and 4,7,4',5'-tetra-chloro-6-(5-carboxypentyl)fluorescein (8b) were synthesized as fluorescent probes for labeling proteins. These two fluoresceins contain 6-aminohexanoic acid as spacer linker to minimize the fluorescence quenching of the fluorescein molecules by the proteins to be labeled.
Tian
Min
M
Department of Chemistry, Northwest University, Xi'an 710069, China.
Wu
Xiang-Long
XL
Zhang
Bin
B
Li
Jian-Li
JL
Shi
Zhen
Z
D001859:Bone Neoplasms / Q000378:metabolism*; D002713:Chlorine / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D005452:Fluoresceins / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D006801:Humans; D015394:Molecular Structure; D012516:Osteosarcoma / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry*; D014407:Tumor Cells, Cultured
C529327:4,7,2',7'-tetrachloro-6-(5-carboxypentyl)fluorescein; C529328:4,7,4',5'-tetra-chloro-6-(5-carboxypentyl)fluorescein; D017931:DNA Primers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D002713:Chlorine