Signal amplification via intramolecular energy transfer using tripodal neutral iridium(III) complexes upon binding to avidin.
A neutral tripod system for biotin-avidin assays, which is composed of biotin, FIrpic as an energy acceptor, and mCP as an energy donor, offers remarkable sensitivity over traditional transition metal based protein probes due to the intramolecular energy transfer and increased hydrophobicity associated with the avidin binding site and neutral probe 1.
Kwon
Tae-Hyuk
TH
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
Kwon
Jongchul
J
Hong
Jong-In
JI
D001360:Avidin / Q000737:chemistry*; D001665:Binding Sites; D001710:Biotin / Q000737:chemistry; D004735:Energy Transfer; D057927:Hydrophobic and Hydrophilic Interactions; D007495:Iridium / Q000737:chemistry*; D049449:Luminescence; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D012680:Sensitivity and Specificity
D009942:Organometallic Compounds; D001360:Avidin; D007495:Iridium; D001710:Biotin