title: Cutting edge: IL-27 is a potent inducer of IL-10 but not FoxP3 in murine T cells.
abstract: The cytokine IL-27 is important for restricting inflammation in response to a wide variety of immune challenges. In this study, we demonstrate that IL-27 induces expression of the anti-inflammatory cytokine IL-10 by CD4+ and CD8+ T cells. IL-27 relied upon the Th1 transcription factor STAT1 to induce IL-10+IFN-gamma+FoxP3- Th1 cells, which were recently shown to be key negative regulators during certain infections. Il27ra-/- mice generated fewer IL-10+ T cells during both Listeria monocytogenes infection and experimental autoimmune encephalomyelitis. The data presented here indicate a novel mechanism for the induction of IL-10 expression by T cells and provide a mechanistic basis for the suppressive effects of IL-27.
authors.affiliation: Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080,USA.
authors.forename: Marcel
authors.initials: M
authors.lastname: Batten
authors.forename: Noelyn M
authors.initials: NM
authors.lastname: Kljavin
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Walter
authors.forename: Frederic J
authors.initials: FJ
authors.lastname: de Sauvage
authors.forename: Nico
authors.initials: N
authors.lastname: Ghilardi
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D002478:Cells, Cultured; D051858:Forkhead Transcription Factors / Q000378:metabolism; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics; D007378:Interleukins / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018121:Receptors, Cytokine / Q000172:deficiency / Q000235:genetics; D018123:Receptors, Interleukin; D050794:STAT1 Transcription Factor / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C486767:Il27 protein, mouse; C484747:Il27ra protein, mouse; D018836:Inflammation Mediators; D007378:Interleukins; D018121:Receptors, Cytokine; D018123:Receptors, Interleukin; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D016753:Interleukin-10; D007371:Interferon-gamma