title: Cloning of miniature pig HIF-1alpha and its responses to immunosuppressive agents.
abstract: Miniature pigs are worth notice as candidate donors for xenotransplantation. However, donor organs are inevitably subjected to hypoxia, which causes vascular endothelial dysfunction. In this respect, hypoxia-inducible factor 1alpha (HIF-1alpha), the key factor for hypoxic adaptation, should be expressed in grafts. However, some immunosuppressive drugs have been reported to suppress HIF-1alpha in rat cells. Here, we first identified the cDNA and protein structures of miniature pig HIF-1alpha, and next investigated the effects of cyclosporine and FK506 on HIF-1alpha expression in endothelial cells of miniature pig. Thus, we conclude that FK506, rather than cyclosporine, may be recommended for xenotransplantation using miniature pig organs.
authors.affiliation: Department of Pharmacology, Seoul National University, College of Medicine, Seoul, Korea.
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Kim
authors.forename: Yu-Jung
authors.initials: YJ
authors.lastname: Jung
authors.forename: Shan Hua
authors.initials: SH
authors.lastname: Li
authors.forename: Yang-Sook
authors.initials: YS
authors.lastname: Chun
authors.forename: Curie
authors.initials: C
authors.lastname: Ahn
authors.forename: Jong-Wan
authors.initials: JW
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis / Q000187:drug effects / Q000235:genetics; D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D003001:Cloning, Molecular; D016572:Cyclosporine / Q000494:pharmacology; D018076:DNA, Complementary; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000187:drug effects; D011487:Protein Conformation; D013552:Swine / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013556:Swine, Miniature / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016559:Tacrolimus / Q000494:pharmacology*; D014183:Transplantation, Heterologous; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D016572:Cyclosporine; D018076:DNA, Complementary; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007166:Immunosuppressive Agents; D016559:Tacrolimus; D000099162:Endothelial PAS Domain-Containing Protein 1