Inhibitory effects of fucoidan on activation of epidermal growth factor receptor and cell transformation in JB6 Cl41 cells.
Algal fucoidan is a marine sulfated polysaccharide with a wide variety of biological activities including anti-thrombotic, anti-inflammatory, and anti-tumor activities. In this study, we tested the hypothesis that fucoidan may suppress neoplastic cell transformation by inhibiting the phosphorylation of epidermal growth factor receptor (EGFR) in mouse epidermal JB6 Cl41 cells. Our results provided the first evidence that fucoidan from Laminaria guryanovae exerted a potent inhibitory effect on EGF-induced phosphorylation of EGFR. Consistent with its inhibitory action on phosphorylation of EGFR, fucoidan clearly suppressed the phosphorylation of extracellular signal-regulated kinase or c-jun N-terminal kinases induced by EGF. Moreover, EGF-induced the c-fos and c-jun transcriptional activities were inhibited by fucoidan, resulting to suppressing of activator protein-1 (AP-1) activity and cell transformation induced by EGF. Taken together, these results indicate that fucoidan might exert chemopreventive effects through the inhibition of phosphorylation of the EGFR.
Lee
Na Yeon
NY
College of Pharmacy, Chosun University, Gwangju, South Korea.
Ermakova
Svetlana P
SP
Zvyagintseva
Tatyana N
TN
Kang
Keon Wook
KW
Dong
Zigang
Z
Choi
Hong Seok
HS
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001711:Biotransformation / Q000187:drug effects; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000187:drug effects*; D017930:Genes, Reporter / Q000187:drug effects; D016762:Genes, fos / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects; D007202:Indicators and Reagents; D007795:Laminaria / Q000737:chemistry; D051379:Mice; D010766:Phosphorylation; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D018808:Transcription Factor AP-1 / Q000187:drug effects / Q000235:genetics
D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D011134:Polysaccharides; D013778:Tetrazolium Salts; D013844:Thiazoles; D018808:Transcription Factor AP-1; D004815:Epidermal Growth Factor; C007789:fucoidan; D066246:ErbB Receptors; C022616:thiazolyl blue