title: Effects of strontium fructose 1,6-diphosphate on expression of apoptosis-related genes and oxidative stress in testes of diabetic rats.
abstract: OBJECTIVE
  To investigate the effects of strontium fructose 1,6-diphosphate (FDP-Sr) on testicular dysfunction induced by diabetes.
  
  
  METHODS
  Diabetes was induced by a single injection of streptozotocin (65 mg/kg, i.p.). After 28 days, therapy with three doses (50, 100, and 200 mg/kg per day, p.o.) of FDP-Sr was carried out for another 4 weeks.
  
  
  RESULTS
  The rats exhibited morphological lesions of testes and significant decreases in serum testosterone levels after 2 months of diabetes. Testicular tissues of diabetic rats showed significantly increased malondialdehyde levels and declined glutathione peroxidase activity. Meanwhile, augmented DNA fragmentation was observed, along with downregulated Bcl-2 and upregulated Bax expressions at both mRNA and protein levels. FDP-Sr showed significant antioxidant effects in both in vitro and in vivo experiments, and significantly relieved apoptosis and the decline of serum testosterone caused by diabetes.
  
  
  CONCLUSIONS
  Testicular injury and apoptosis induced by diabetes are partially attributed to the augmented oxidative stress in testicular tissue. FDP-Sr indirectly alleviates these pathologic alterations by suppressing the generation of reactive oxygen species.
authors.affiliation: Research Division of Pharmacology, China Pharmaceutical University, Nanjing, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Tang
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: De-Zai
authors.initials: DZ
authors.lastname: Dai
authors.forename: Han-Jie
authors.initials: HJ
authors.lastname: Ying
authors.forename: Qiu-Juan
authors.initials: QJ
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology*; D006597:Fructose-Bisphosphatase / Q000494:pharmacology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013324:Strontium / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects*
chemical_list: D006597:Fructose-Bisphosphatase; D013324:Strontium