title: Light damaging action of all-trans-retinal and its derivatives on rhodopsin molecules in the photoreceptor membrane.
abstract: We have reproduced the model system containing A2-rhodopsin, NR-PE, A2-PE, and ATR-dimer-PE in order to study photosensitized damage of rhodopsin within photoreceptor membranes of rod outer segments. We have demonstrated that irradiation of such a system with visible light (400-700 nm) distorts the most important functional property of native visual pigment--its ability to regenerate after addition of 11-cis-retinal in the dark. We have also shown that all-trans-retinal bound to membrane phospholipids and rhodopsin has less photosensitizing activity that free all-trans-retinal.
authors.affiliation: Emanuel' Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia. marina.loguinova@mail.ru
authors.forename: M Yu
authors.initials: MY
authors.lastname: Loginova
authors.forename: Ye V
authors.initials: YV
authors.lastname: Rostovtseva
authors.forename: T B
authors.initials: TB
authors.lastname: Feldman
authors.forename: M A
authors.initials: MA
authors.lastname: Ostrovsky
mesh_terms: D007536:Isomerism; D008027:Light; D008563:Membrane Lipids / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011726:Pyridinium Compounds / Q000737:chemistry; D012172:Retinaldehyde / Q000031:analogs & derivatives / Q000737:chemistry* / Q000528:radiation effects*; D012176:Retinoids / Q000737:chemistry; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: C511385:A2-PE compound; C481026:A2-rhodopsin; D008563:Membrane Lipids; C497343:N-retinylidene-phosphatidylethanolamine; D010714:Phosphatidylethanolamines; D011726:Pyridinium Compounds; D012176:Retinoids; C525877:retinal dimer phosphatidylethanolamine; D012243:Rhodopsin; D012172:Retinaldehyde