Inhibition of DLC-1 gene expression by RNA interference in the colon cancer LoVo cell line.
DLC-1 (deleted in liver cancer-1) is a potential tumor suppressor gene, which is inactive in liver carcinogenesis. To observe the effects of DLC-1 gene expression on cell proliferation and migration in the human colon cancer cell line, RNAi Lipo-recombinant of the DLC-1 gene (pGCsil-DLC-1) was constructed and transduced into LoVo cells which are positive for DLC-1 gene expression. Results showed that the RNAi recombinant effectively inhibited the expression of the DLC-1 gene in LoVo cells. Additionally, our data showed decreased DLC-1 gene expression which resulted in the promotion of LoVo cell proliferation. Flow cytometry in cell cycle detection further indicated that the DLC-1 gene induced cell cycle arrest at G2/M and a cell migration assay confirmed that the knocking down of DLC-1 gene expression promotes LoVo cell migration. Our observations suggest that the DLC-1 gene is associated with LoVo cell proliferation, migration and cell cycle distribution. DLC-1 is a potential suppressor gene in the colon cancer LoVo cell line and may play an important role in colon cancer mechanisms.
Jin
Yueling
Y
Department of Clinical Medicine, School of Clinical Medicine, Southeast University, Nanjing 210009, P.R. China.
Tian
Xiaoqiang
X
Shang
Yanfang
Y
Huang
Peiling
P
D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D020690:GTPase-Activating Proteins; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D022661:RNA, Untranslated / Q000378:metabolism; D014410:Tumor Stem Cell Assay; D025521:Tumor Suppressor Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*
C112352:DLC1 protein, human; D020690:GTPase-Activating Proteins; D022661:RNA, Untranslated; D025521:Tumor Suppressor Proteins