Introduction of silencing-inducing transgene against Fgf19 does not affect expression of Tbx5 and beta3-tubulin in the developing chicken retina.
Fgf19 is known to be expressed in the developing chicken eye but its functions during retinal development have remained elusive. Since Fgf19 is expressed in the dorsal portion of the optic cup, it is intriguing to know whether FGF19 is required for expression of dorso-ventral morphogenetic genes in the eye. To clarify this, expression patterns of Tbx5 and Vax were examined in the developing eye after in ovo RNA interference targeted against Fgf19. Quantitative polymerase chain reaction (PCR) analysis showed that the short-hairpin RNAs (shRNAs) targeted against Fgf19 could reduce its expression in the eye to less than 50% of a relative amount of mRNA, compared with contralateral or untreated control eyes. However, no obvious alteration in expression domains of Tbx5 or Vax was observed. Misexpression of Tbx5 or Tbx5-RNAi did not alter the Fgf19 expression either. Furthermore, although Fgf19 is expressed in the central retina before neurogenesis occurs, beta3-tubulin, a marker for early retinal differentiation was still detected in the central retina after knockdown of Fgf19. Thus, knockdown of Fgf19 supports no obvious regulations between Fgf19 and Tbx5, or exhibits no phenotypes that perturb early retinal differentiation.
Okamoto
Mayumi
M
Department of Life Systems, Institute of Technology and Science, The University of Tokushima, 2-1 Minami-Josanjima-cho, Tokushima City 770-8506, Japan.
Tomonari
Sayuri
S
Naito
Yuki
Y
Saigo
Kaoru
K
Noji
Sumihare
S
Ui-Tei
Kumiko
K
Ohuchi
Hideyo
H
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018274:Electroporation; D005136:Eye Proteins / Q000096:biosynthesis* / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D009024:Morphogenesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering / Q000235:genetics; D012160:Retina / Q000196:embryology* / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000096:biosynthesis* / Q000235:genetics; D019076:Transgenes; D014404:Tubulin / Q000096:biosynthesis* / Q000235:genetics
D005136:Eye Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5; D014404:Tubulin; D005346:Fibroblast Growth Factors