title: Acetaldehyde differentially affects the growth of uterine leiomyomata and myometrial cells in tissue cultures.
abstract: OBJECTIVE
  To examine the effect of alcohol and its derivatives on leiomyomata versus normal myometrium in cell culture.
  
  
  METHODS
  A study on human tissue cultures.
  
  
  METHODS
  A tertiary-care university hospital.
  
  
  METHODS
  Premenopausal women with uterine myomas.
  
  
  METHODS
  Obtaining paired cultures of normal myometrium and myomas from women undergoing hysterectomy.
  
  
  METHODS
  Analysis of the effect of ethanol and acetaldehyde on cell growth and the expression of relevant proteins.
  
  
  RESULTS
  Acetaldehyde statistically significantly inhibited the growth of myoma cells compared with normal myometrium. The level of alcohol dehydrogenase 1 (ADH1) protein was lower in myoma than in myometrial cells. The acetaldehyde dehydrogenase 1 (ALDH1) protein level was higher in myoma cells. Treatment with acetaldehyde resulted in a relative reduction of ALDH1 level in the myoma cells.
  
  
  CONCLUSIONS
  Acetaldehyde has an inhibitory effect on cell growth of myoma compared with normal myometrium. The reduced level of ADH1 and the increased level of ALDH1 proteins observed in myoma cell culture reduces the acetaldehyde level and thus may be involved in myoma cell growth.
authors.affiliation: Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, Ein-Kerem, Jerusalem, Israel. dshveiky@gmail.com
authors.forename: David
authors.initials: D
authors.lastname: Shveiky
authors.forename: Asher
authors.initials: A
authors.lastname: Shushan
authors.forename: Hannah
authors.initials: H
authors.lastname: Ben Bassat
authors.forename: Benjamin Y
authors.initials: BY
authors.lastname: Klein
authors.forename: Assaf
authors.initials: A
authors.lastname: Ben Meir
authors.forename: Rubina
authors.initials: R
authors.lastname: Levitzky
authors.forename: Nathan
authors.initials: N
authors.lastname: Rojansky
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism / Q000633:toxicity*; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007889:Leiomyoma / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D009215:Myometrium / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D050697:Retinal Dehydrogenase; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*
chemical_list: D007527:Isoenzymes; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase; D000079:Acetaldehyde