UTILLdb, a Pisum sativum in silico forward and reverse genetics tool.
The systematic characterization of gene functions in species recalcitrant to Agrobacterium-based transformation, like Pisum sativum, remains a challenge. To develop a high throughput forward and reverse genetics tool in pea, we have constructed a reference ethylmethane sulfonate mutant population and developed a database, UTILLdb, that contains phenotypic as well as sequence information on mutant genes. UTILLdb can be searched online for TILLING alleles, through the BLAST tool, or for phenotypic information about mutants by keywords.
Dalmais
Marion
M
Unité de Recherche en Génomique Végétale, UMR INRA-CNRS, Rue Gaston Crémieux, 91057 Evry Cedex, France. dalmais@evry.inra.fr
Schmidt
Julien
J
Le Signor
Christine
C
Moussy
Francoise
F
Burstin
Judith
J
Savois
Vincent
V
Aubert
Gregoire
G
Brunaud
Veronique
V
de Oliveira
Yannick
Y
Guichard
Cecile
C
Thompson
Richard
R
Bendahmane
Abdelhafid
A
D000483:Alleles; D004252:DNA Mutational Analysis / Q000379:methods*; D030541:Databases, Genetic; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D017343:Genes, Plant; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D018532:Pisum sativum / Q000033:anatomy & histology / Q000187:drug effects / Q000235:genetics*
D009153:Mutagens; D005020:Ethyl Methanesulfonate