Expression of interleukin-8 and monocyte chemotactic protein 1 in women with endometriosis.
OBJECTIVE
To investigate the expression and localization of interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) in women with and without endometriosis.


METHODS
Comparative immunohistochemical study.


METHODS
Academic medical center.


METHODS
Ectopic (n = 24) and homologous eutopic endometrium (n = 24) from women with endometriosis and endometrium from women without endometriosis (n = 27) were used for immunohistochemical analysis of IL-8 and MCP-1.


METHODS
Tissue sections were immunostained with antihuman IL-8 and MCP-1 antibodies.


METHODS
Microscopic evaluation to assess the presence and localization of IL-8 and MCP-1 throughout the menstrual cycle in both eutopic endometrial and endometriotic tissues of women with endometriosis and comparison with normal endometrium.


RESULTS
In normal endometrium, secretory phase samples expressed higher levels of epithelial IL-8 than in proliferative phase samples. Epithelial MCP-1 expression was similar in both proliferative and secretory phases. Proliferative phase samples showed higher epithelial IL-8 and MCP-1 expressions in eutopic endometrium of women with endometriosis compared with that of normal women. Immunoreactivities of both chemokines were significantly increased in the epithelial cells of ectopic endometrial tissues compared with those of normal endometrium.


CONCLUSIONS
These findings suggest that IL-8 and MCP-1 may be involved in the pathogenesis of endometriosis.
Ulukus
Murat
M
Department of Obstetrics & Gynecology, Ege University School of Medicine, Bornova, Izmir, Turkey.
Ulukus
E Cagnur
EC
Tavmergen Goker
Ege N
EN
Tavmergen
Erol
E
Zheng
Wenxin
W
Arici
Aydin
A
D000328:Adult; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000032:analysis*; D004715:Endometriosis / Q000276:immunology* / Q000473:pathology; D004717:Endometrium / Q000276:immunology* / Q000473:pathology; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000032:analysis*; D008875:Middle Aged; D012189:Retrospective Studies; D017154:Stromal Cells / Q000276:immunology
C111835:CCL2 protein, human; C508606:CXCL8 protein, human; D018932:Chemokine CCL2; D016209:Interleukin-8