Comparison of in vivo translocation frequencies with in vitro G2 radiosensitivity in radiation workers occupationally exposed to external radiation.
A group of retired workers from the British Nuclear Fuels plc facility at Sellafield who had been studied for in vivo translocation frequencies in blood lymphocytes were resampled and analysed for in vitro chromosomal radiosensitivity. Significant variation in response to a dose of 0.5 Gy given at the G(2) stage of the cell cycle was observed between individuals (P < 0.001). In a regression analysis that included age, cumulative occupational radiation dose and in vitro G(2) radiation-induced aberration frequencies as independent variables, only cumulative occupational radiation dose had a significant influence on chromosomal translocation frequency (P = 0.0036). G(2) in vitro radiosensitivity is assumed to be a marker for genetic polymorphic variation in DNA damage recognition and repair genes. Therefore, since in vivo translocation frequencies can be considered a surrogate for cancer risk, this lack of association with G(2) in vitro radiosensitivity suggests that such genetic variation has no impact on the response to low dose chronic exposure.
Cadwell
Kevin K
KK
Westlakes Research Institute, Moor Row, Cumbria, UK.
Whitehouse
Caroline A
CA
Tarone
Robert E
RE
Janet Tawn
E
E
D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D005373:Film Dosimetry; D016195:G2 Phase; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009689:Nuclear Warfare; D016273:Occupational Exposure; D011210:Power Plants; D011829:Radiation Dosage; D014178:Translocation, Genetic; D006113:United Kingdom