title: Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage.
abstract: A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.
authors.affiliation: Waksman Institute for Microbiology, Piscataway, NJ 08854, USA.
authors.forename: Dhruti
authors.initials: D
authors.lastname: Savalia
authors.forename: Lars F
authors.initials: LF
authors.lastname: Westblade
authors.forename: Manisha
authors.initials: M
authors.lastname: Goel
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Priscilla
authors.initials: P
authors.lastname: Kemp
authors.forename: Natalja
authors.initials: N
authors.lastname: Akulenko
authors.forename: Olga
authors.initials: O
authors.lastname: Pavlova
authors.forename: Julio C
authors.initials: JC
authors.lastname: Padovan
authors.forename: Brian T
authors.initials: BT
authors.lastname: Chait
authors.forename: Michael P
authors.initials: MP
authors.lastname: Washburn
authors.forename: Hans-W
authors.initials: HW
authors.lastname: Ackermann
authors.forename: Arcady
authors.initials: A
authors.lastname: Mushegian
authors.forename: Tarasii
authors.initials: T
authors.lastname: Gabisonia
authors.forename: Ian
authors.initials: I
authors.lastname: Molineux
authors.forename: Konstantin
authors.initials: K
authors.lastname: Severinov
mesh_terms: D003090:Coliphages / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D023281:Genomics; D011401:Promoter Regions, Genetic; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D020543:Proteome; D012321:DNA-Directed RNA Polymerases