title: Direct evidence for ArO-S bond cleavage upon inactivation of Pseudomonas aeruginosa arylsulfatase by aryl sulfamates.
abstract: Pseudomonas aeruginosa arylsulfatase catalyses the cleavage of aryl sulfates and is an excellent model for human estrone sulfatase, which is implicated in hormone-dependent breast cancer. Aryl sulfamates are inactivators of sulfatases; however, little is known about their mechanism. We studied the inactivation of Pseudomonas aeruginosa arylsulfatase A by a range of aryl sulfamates, including the clinical agent 667COUMATE (STX64) used to inactivate estrone sulfatase. Inactivation was time dependent, irreversible, and active-site directed, consistent with a covalent modification at the active site. In terms of the kinetic parameters of inactivation k(inact) and K(i), K(i) values are in the micromolar to nanomolar range, and the inactivation half-life is less than 30 s. A Brønsted plot of k(inact)/K(i) has a steep slope (beta(lg) = -1.1), which implies that the transition state for the first irreversible chemical step of inactivation involves a high degree of charge transfer and cleavage of the ArO-S bond. Detection of the released phenol and titration of the residual activity showed the stoichiometry of inactivation to be in the range 3-6, with the greatest values found for the most effective inactivators. Thus, multiple sulfamoylation events appear to occur during the inactivation process. These data provide valuable insight into the mechanism of sulfatase inactivation by sulfamates.
authors.affiliation: School of Chemistry and Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 30 Flemington Road, 3010 Parkville, Victoria, Australia.
authors.forename: Pavla
authors.initials: P
authors.lastname: Bojarová
authors.forename: Emma
authors.initials: E
authors.lastname: Denehy
authors.forename: Ian
authors.initials: I
authors.lastname: Walker
authors.forename: Karen
authors.initials: K
authors.lastname: Loft
authors.forename: David P
authors.initials: DP
authors.lastname: De Souza
authors.forename: L W Lawrence
authors.initials: LW
authors.lastname: Woo
authors.forename: Barry V L
authors.initials: BV
authors.lastname: Potter
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: McConville
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Williams
mesh_terms: D001128:Argon / Q000737:chemistry*; D001192:Arylsulfatases / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry*; D019800:Phenol / Q000737:chemistry / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000737:chemistry* / Q000378:metabolism*; D013455:Sulfur / Q000737:chemistry*; D017186:Titrimetry
chemical_list: D013451:Sulfonic Acids; D019800:Phenol; D001128:Argon; D013455:Sulfur; C005741:sulfamic acid; D001192:Arylsulfatases; D010100:Oxygen