Fully automated [18F]fluorocholine synthesis in the TracerLab MX FDG Coincidence synthesizer.
BACKGROUND
We developed a new fully automated method for the radiosynthesis of [18F]fluorocholine by modifying the commercial 2-[18F]fluoro-2-d-deoxy-glucose ([18F]FDG) synthesizer module (GE TracerLab MX, formerly Coincidence).


METHODS
[18F]Flurocholine was synthesized by (18)F-fluoroalkylation of N,N-dimethylaminoethanol using [18F]fluorobromomethane as fluoromethylating agent. [18F]Fluorobromomethane was produced by reaction of dibromomethane with [18F]fluoride, assisted by Kryptofix 2.2.2.


RESULTS
After purification on solid-phase extraction cartridges, the [18F]fluorocholine was obtained in 15-25% radiochemical yields (decay not corrected), with more than 99% radiochemical purity. Specific activity was more than 37 GBq/micromol. Synthesis time was less than 35 min.


CONCLUSIONS
This new automated synthesis technique provides high and reproducible yields that could be dedicated for routine use with the same [18F]FDG disposable cassette system.
Kryza
David
D
Laboratoire CREATIS-ANIMAGE, UMR 5515 Cnrs-U630 Inserm-Insa de Lyon, France. david.kryza@chu-lyon.fr
Tadino
Vincent
V
Filannino
Maria Azzurra
MA
Villeret
Guillaume
G
Lemoucheux
Laurent
L
D000478:Alkylation; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002794:Choline / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D004867:Equipment Design; D005462:Fluorine Radioisotopes / Q000737:chemistry; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry; D008745:Methylation; D011854:Radiochemistry / Q000295:instrumentation* / Q000379:methods*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis
D019086:Bridged Bicyclo Compounds, Heterocyclic; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; C006071:cryptating agent 222; C514960:fluorocholine; D002794:Choline