title: Separation efficiencies in hydrophilic interaction chromatography.
abstract: Hydrophilic interaction chromatography (HILIC) is important for the separation of highly polar substances including biologically active compounds, such as pharmaceutical drugs, neurotransmitters, nucleosides, nucleotides, amino acids, peptides, proteins, oligosaccharides, carbohydrates, etc. In the HILIC mode separation, aqueous organic solvents are used as mobile phases on more polar stationary phases that consist of bare silica, and silica phases modified with amino, amide, zwitterionic functional group, polyols including saccharides and other polar groups. This review discusses the column efficiency of HILIC materials in relation to solute and stationary phase structures, as well as comparisons between particle-packed and monolithic columns. In addition, a literature review consisting of 2006-2007 data is included, as a follow up to the excellent review by Hemström and Irgum.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. ikegami@kit.ac.jp
authors.forename: Tohru
authors.initials: T
authors.lastname: Ikegami
authors.forename: Kouki
authors.initials: K
authors.lastname: Tomomatsu
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Takubo
authors.forename: Kanta
authors.initials: K
authors.lastname: Horie
authors.forename: Nobuo
authors.initials: N
authors.lastname: Tanaka
mesh_terms: D000085:Acetates / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D044948:Flavonols / Q000302:isolation & purification; D009844:Oligosaccharides / Q000302:isolation & purification; D011687:Purines / Q000302:isolation & purification; D011743:Pyrimidines / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature; D013754:Tetracyclines / Q000302:isolation & purification
chemical_list: D000085:Acetates; D044948:Flavonols; D009844:Oligosaccharides; D011687:Purines; D011743:Pyrimidines; D013754:Tetracyclines; D012822:Silicon Dioxide