Alcoholic chestnut fermentation in mixed culture. Compatibility criteria between Aspergillus oryzae and Saccharomyces cerevisiae strains.
The main objective of the present work consisted in the transfer to the case of the chestnut of a rice fermentative process that carried out to the Japanese traditional way to lead to an alcoholic bagasse, the moromi, capable of obtaining distilled. This way, selection assays of amylolitic Aspergillus oryzae strains and studies of compatibility between microfungi and yeast were carried out. These mixed cultivations were performed operating in batch submerged culture. Later on, using solid state system (chestnut, microfungi, yeast), a fermentative fed-batch process (koji, moto, moromi) was defined. By means of this approach a yield of 70% was reached in the conversion of total carbohydrates in ethanol. Also, the time required by the traditional operation was reduced in half.
Murado
Miguel Anxo
MA
Grupo de Reciclado y Valorización de Materiales Residuales, Instituto de Investigacións Mariñas (CSIC), r/Eduardo Cabello, 6, Vigo 36208, Galicia, Spain.
Pastrana
Lorenzo
L
Vázquez
José Antonio
JA
Mirón
Jesús
J
González
María Pilar
MP
D001236:Aspergillus oryzae / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012680:Sensitivity and Specificity
D000431:Ethanol