Lipid, protein, DNA oxidation and antioxidant status in rheumatoid arthritis.
OBJECTIVE
To investigate lipid, protein, DNA oxidation and antioxidant status in blood and synovial fluid of rheumatoid arthritis (RA) patients and to determine the importance of oxidative stress parameters in reflecting disease activity.


METHODS
20 RA patients and 15 healthy controls were included. Lipid peroxidation (thiobarbituric acid reactive substances (TBARS), lipid hydroperoxide, and conjugated diene), protein oxidation (carbonyl and thiol), DNA oxidation (8-OHdG) and antioxidant status markers (glutathione (GSH), glutathione peroxidase (GSH Px), superoxide dismutase (CuZn SOD), and catalase) were determined in blood and synovial fluid.


RESULTS
TBARS (p<0.001), lipid hydroperoxide (p<0.001), conjugated diene (p<0.001), carbonyl (p<0.001) and 8-OHdG (p<0.01) levels were significantly higher; thiol (p<0.01) and GSH levels (p<0.01) and GSH Px (p<0.001) and CuZn SOD (p<0.01) activities were significantly lower in blood of RA patients. TBARS (p<0.001), lipid hydroperoxide (p<0.001), conjugated diene (p<0.01), carbonyl (p<0.001) and 8-OHdG (p<0.05) levels were significantly higher, catalase activity (p<0.001) significantly lower in synovial fluid of RA patients.


CONCLUSIONS
Increased lipid, protein and DNA oxidation markers and impaired antioxidant status confirm the role of oxidative stress in the pathogenesis of RA. Lipid peroxidation markers can serve as surrogate markers for disease activity.
Seven
Arzu
A
Department of Biochemistry, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey. arzu_seven@hotmail.com
Güzel
Savaş
S
Aslan
Mahmure
M
Hamuryudan
Vedat
V
D000328:Adult; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000235:genetics; D008055:Lipids / Q000032:analysis / Q000235:genetics / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000235:genetics; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D008055:Lipids; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; D004247:DNA