Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles.
A recently described technique [Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152-160] for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an iso-osmolar glycerol solution, a value for the permeability of POPC membrane for glycerol, P=(2.09+/-0.82) x 10(-8)m/s, has been obtained. Based on this result, an alternative mechanism is proposed for the observed exchange of vesicle interior. With modifications, the method of Estes and Mayer is then applied to preparation of flaccid GUVs.
Peterlin
Primoz
P
University of Ljubljana, Faculty of Medicine, Institute of Biophysics, Lipiceva 2, SI-1000 Ljubljana, Slovenia. primoz.peterlin@biofiz.mf.uni-lj.si
Arrigler
Vesna
V
D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D005990:Glycerol; D010539:Permeability; D019999:Pharmaceutical Solutions; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000138:chemical synthesis / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000138:chemical synthesis* / Q000737:chemistry
D019999:Pharmaceutical Solutions; D010713:Phosphatidylcholines; D010743:Phospholipids; D053835:Unilamellar Liposomes; D005990:Glycerol; C028694:1-palmitoyl-2-oleoylphosphatidylcholine