[Study on interference therapy induced by epidermal growth factor receptor-antisense cDNA in signal transduction of laryngeal squamous cell carcinoma].
OBJECTIVE
To investigate effect of interference therapy induced by epidermal growth factor receptor (EGFR)-antisense cDNA in signal transduction of Hep-2 laryngeal squamous cell carcinoma in vitro.


METHODS
AdEasy Vector System was used to construct the recombinant adenovirus vector sense/antisense-pAdEasy-EGFR. The recombinant adenovirus vector introduced EGFR-sense/antisense cDNA fragment into HEK293 cell. The purified recombinant adenovirus sense/antisense-pAdEasy-EGFR transfected Hep-2 cells in vitro. The inhibition of EGFR protein expression and proliferation of Hep-2 cells, the changes of cell cycle and DNA content in Hep-2 cells were examined by MTT, Western blot analysis, flow cytometry essay, and immunocytochemistry respectively.


RESULTS
The higher titre sense and antisense mRNA expression recombinant adenovirus containing 1,032 bp EGFR-cDNA was constructed and prepared successfully. When antisense-pAdEasy-EGFR was transferred into Hep-2 cells the inhibition of cell proliferation and EGFR protein expression in Hep-2 cells were investigated effectively.


CONCLUSIONS
The antisense-pAdEasy-EGFR effectively interfere the Hep-2 signal transduction pathway and induce apoptosis which results in inhibiting proliferation of laryngeal squamous cell carcinoma.
Xian
Jun-Ming
JM
Dept of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Guang-Yao
GY
Liang
Chuan-Yu
CY
Liu
Shi-Xi
SX
D000256:Adenoviridae; D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D002453:Cell Cycle; D049109:Cell Proliferation; D018076:DNA, Complementary; D066246:ErbB Receptors; D005822:Genetic Vectors; D057809:HEK293 Cells; D006258:Head and Neck Neoplasms; D006801:Humans; D007822:Laryngeal Neoplasms; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck; D014162:Transfection
D018076:DNA, Complementary; D066246:ErbB Receptors