[Effects of two different cryoprotectants on the motility of post-thaw human sperm].
OBJECTIVE
To compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm.


METHODS
The cryoprotectant containing glucose and that containing sucrose were applied to 50 semen samples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups.


RESULTS
The forward motility and total motility of the sperm were (58.4 +/- 5.7)% and (63.4 +/- 6.1)% before cryopreservation, (43.8 +/- 7.6)% and (48.4 +/- 7.6)% after thawing with the cryoprotectant containing glucose, and(42.6 +/- 8.9)% and (48.0 +/- 8.5)% after thawing with the cryoprotectant containing sucrose. Decreased sperm motility was observed after cryopreservation, with statistic significance (P < 0.01). There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryoprotectants.


CONCLUSIONS
Cryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.
Hu
Jing-Mei
JM
Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan, Shandong 250021, China.
Xu
Cheng-Yan
CY
Li
Yuan
Y
Lu
Shao-Ming
SM
Wang
Li
L
Chen
Zi-Jiang
ZJ
D000328:Adult; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology; D013395:Sucrose / Q000494:pharmacology*
D003451:Cryoprotective Agents; D013395:Sucrose; D005947:Glucose