[Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1].
OBJECTIVE
To investigate the regulation effects of upstream stimulatory factor 1 (USF1) on osteopontin expression in odontoblasts.


METHODS
Odontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed.


RESULTS
Clones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group.


CONCLUSIONS
Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF.
Wu
Li-An
LA
Department of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Wen
Ling-Ying
LY
Yang
Fu-Sheng
FS
D045744:Cell Line, Tumor; D006801:Humans; D009804:Odontoblasts / Q000378:metabolism*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D051720:Upstream Stimulatory Factors / Q000235:genetics*
D012333:RNA, Messenger; C497181:USF1 protein, human; D051720:Upstream Stimulatory Factors; D053495:Osteopontin