title: Synthesis of chlorinated fluoresceins for labeling proteins.
abstract: Two novel chlorinated fluoresceins 4,7,2',7'-tetrachloro-6-(5-carboxypentyl)fluorescein (8a) and 4,7,4',5'-tetra-chloro-6-(5-carboxypentyl)fluorescein (8b) were synthesized as fluorescent probes for labeling proteins. These two fluoresceins contain 6-aminohexanoic acid as spacer linker to minimize the fluorescence quenching of the fluorescein molecules by the proteins to be labeled.
authors.affiliation: Department of Chemistry, Northwest University, Xi'an 710069, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tian
authors.forename: Xiang-Long
authors.initials: XL
authors.lastname: Wu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shi
mesh_terms: D001859:Bone Neoplasms / Q000378:metabolism*; D002713:Chlorine / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D005452:Fluoresceins / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D006801:Humans; D015394:Molecular Structure; D012516:Osteosarcoma / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: C529327:4,7,2',7'-tetrachloro-6-(5-carboxypentyl)fluorescein; C529328:4,7,4',5'-tetra-chloro-6-(5-carboxypentyl)fluorescein; D017931:DNA Primers; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011506:Proteins; D002713:Chlorine