title: Immunohistochemical assessment of E-cadherin and beta-catenin in trichofolliculomas and trichoepitheliomas.
abstract: BACKGROUND
  Trichofolliculomas and trichoepitheliomas are benign skin neoplasms originating from hair follicle cells. They result from defects in the signaling pathways that regulate hair follicle morphogenesis and regeneration. Thus they seem to be an excellent model of these processes. It is known that the E-cadherin/beta-catenin system of adhesion molecules plays a crucial role in the maintenance of tissue architecture.
  
  
  OBJECTIVE
  The aim of the present study was to investigate their involvement in benign hair follicle tumor development.
  
  
  METHODS
  Semiquantitative intensity of expression were examined in formalin-fixed and paraffin-embedded tissue sections of 53 trichoepitheliomas, 15 trichofolliculomas and 19 normal skin samples by indirect immunohistochemistry.
  
  
  RESULTS
  The intensity of E-cadherin/beta-catenin expression in tumor cells did not differ from controls. However, normal hair follicles cells exhibited membranous E-cadherin/beta-catenin expression, whereas both types of tumors, particularly trichoepitheliomas, showed E-cadherin/beta-catenin expression with a predominantly cytoplasmic localization.
  
  
  CONCLUSIONS
  We suggest that this dystopic distribution of the E-cadherin/beta-catenin complex in hair follicle tumor cells may be a marker of cell-cell adhesion disruption which may contribute to the tumor formation.
authors.affiliation: Department of Pathology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic. mfiuraskova@seznam.cz
authors.forename: Michala
authors.initials: M
authors.lastname: Bezdekova
authors.forename: Svetlana
authors.initials: S
authors.lastname: Brychtova
authors.forename: Eva
authors.initials: E
authors.lastname: Sedlakova
authors.forename: Jana
authors.initials: J
authors.lastname: Steigerova
authors.forename: Alice
authors.initials: A
authors.lastname: Hlobilkova
authors.forename: Martina
authors.initials: M
authors.lastname: Bienova
authors.forename: Renata
authors.initials: R
authors.lastname: Kucerova
authors.forename: Tomas
authors.initials: T
authors.lastname: Brychta
authors.forename: Veronika
authors.initials: V
authors.lastname: Krejci
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Kolar
mesh_terms: D015820:Cadherins / Q000032:analysis*; D006201:Hair Diseases / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018295:Neoplasms, Basal Cell / Q000737:chemistry*; D012878:Skin Neoplasms / Q000737:chemistry*; D051176:beta Catenin / Q000032:analysis*
chemical_list: D015820:Cadherins; D051176:beta Catenin