Detection and quantitation of Solenopsis invicta virus-2 genomic and intermediary replicating viral RNA in fire ant workers and larvae.
Quantitative real-time PCR (QPCR) was used to quantify the genome of Solenopsis invicta virus-2 (SINV-2) from infected individual ants of S. invicta. Strand-specific cDNA synthesis oligonucleotide primers and RNase digestion after cDNA synthesis allowed quantification of plus (genomic) and minus (replicative) strands of the SINV-2 genome. Both strands were detected in adult workers and larval fire ants indicating that the virus was replicating within the ant. The differences between the genomic to replicative strand ranged from 199-fold in larvae to 479-fold in workers with an average ratio of 339:1.
Hashimoto
Yoshifumi
Y
Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 1600 SW 23rd Drive, Gainesville, FL 32608, USA.
Valles
Steven M
SM
D000818:Animals; D001000:Ants / Q000821:virology*; D005814:Genes, Viral; D016679:Genome, Viral; D007304:Insect Viruses / Q000235:genetics*; D007814:Larva / Q000821:virology; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication
D012367:RNA, Viral