title: Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into vitrified human oocytes.
abstract: OBJECTIVE
  To describe the birth achieved from frozen embryos after intracytoplasmic sperm injection (ICSI) of donor sperm into vitrified oocytes.
  
  
  METHODS
  A 25-year-old woman whose husband was azoospermic undergoing IVF therapy.
  
  
  METHODS
  Oocytes collected after ovarian stimulation were vitrified, thawed, and fertilized by frozen donor sperm.
  
  
  RESULTS
  Nineteen oocytes were vitrified and all survived after thawing. Thirteen of the 19 oocytes that underwent ICSI with donors sperm were successfully fertilized. Twelve embryos were cryopreserved again by conventional slow-freezing protocol because of uterine bleeding on the day of transfer. Three thawed embryos were transferred, and a normal male with an infant karyotype of 46,XY was delivered.
  
  
  CONCLUSIONS
  This case report demonstrates effective oocyte cryopreservation by vitrification.
authors.affiliation: Department of Obstetrics & Gynecology, Third Hospital, Peking University, Peking, China. chenguian@bjmu.edu.cn
authors.forename: Gui An
authors.initials: GA
authors.lastname: Chen
authors.forename: Xue Yong
authors.initials: XY
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Lian
authors.forename: Xiao Ying
authors.initials: XY
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
authors.forename: Xin Na
authors.initials: XN
authors.lastname: Chen
authors.forename: Rong Hua
authors.initials: RH
authors.lastname: Ye
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000628:therapy; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D014019:Tissue Donors