Factor analysis scales of generalized amino acid information as applied in predicting interactions between the human amphiphysin-1 SH3 domains and their peptide ligands.
Factor analysis scales of generalized amino acid information (FASGAI) involving hydrophobicity, alpha-helix and beta-turn propensities, bulky properties, compositional characteristics, local flexibility, and electronic properties, was proposed to represent the structures of the decapeptides binding the human amphiphysin-1 SH3 domains. Parameters being responsible for the binding affinities were selected by genetic algorithm, and a quantitative structure-affinity relationship (QSAR) model by partial least square was established to predict the peptide-SH3 domain interactions. Diversified properties of the residues between P(2) and P(-3) (including P(2) and P(-3)) of the decapeptide (P(4)P(3)P(2)P(1)P(0)P(-1)P(-2)P(-3)P(-4)P(-5)) may contribute remarkable effect to the interactions between the SH3 domain and the decapeptide. Particularly, electronic properties of P(2) may provide relatively large positive contributions to the interactions, and reversely, hydrophobicity of P(2) may be largely negative to the interactions. These results showed that FASGAI vectors can well represent the structural characteristics of the decapeptides. Furthermore, the model obtained, which showed low computational complexity, correlated FASGAI descriptors with the binding affinities as well as that FASGAI vectors may also be applied in QSAR studies of peptides.
Liang
Guizhao
G
College of Bioengineering, Chongqing University, Chongqing 400030, PR China. sdqdlgz@163.com
Chen
Guohua
G
Niu
Weihuan
W
Li
Zhiliang
Z
D000596:Amino Acids / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008956:Models, Chemical; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D025941:Protein Interaction Mapping; D021281:Quantitative Structure-Activity Relationship; D018909:src Homology Domains
D000596:Amino Acids; D008024:Ligands; D009419:Nerve Tissue Proteins; D010455:Peptides; C075637:amphiphysin