Beta-catenin siRNA inhibits ischemia-induced striatal neurogenesis in adult rat brain following a transient middle cerebral artery occlusion.
Beta-catenin, a protein that functions in both cell adhesion and Wnt signaling, plays vital roles in mammalian neural development. To investigate the roles of beta-catenin in stroke-induced neurogenesis, we injected beta-catenin siRNA into ipsilateral ischemic lateral ventricle. We found that inactivation of beta-catenin by siRNA caused the decline of beta-catenin in the ischemic striatum, enlarged stroke-induced infarct volume, reduced SVZ expansion, and inhibited striatal neurogenesis in adult rat brain following a transient middle cerebral artery occlusion (tMCAO). These results show that beta-catenin-mediated transcriptional activation functions in the decision of subventricular zone precursors to proliferate or differentiate during stroke-induced striatal neurogenesis, and suggest that the signaling activity of beta-catenin may control the production of newborn neurons and thus regulate the autonomous repair in the striatum after ischemia.
Lei
Zhi-Nian
ZN
Department of Neurobiology and Institute for Biomedical Science, State Key Laboratory of Medical Neurobiology, Shanghai Medical College of Fudan University, 138, Yi-Xue-Yuan Road, Shanghai 200032, PR China.
Zhang
Lin-Mei
LM
Sun
Feng-Yan
FY
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000187:drug effects*; D005452:Fluoresceins; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology*; D007511:Ischemia / Q000473:pathology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009930:Organic Chemicals; D034741:RNA, Small Interfering / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
D005452:Fluoresceins; D009419:Nerve Tissue Proteins; D009930:Organic Chemicals; D034741:RNA, Small Interfering; D014404:Tubulin; D051176:beta Catenin; C435731:fluoro jade; D001973:Bromodeoxyuridine