[Genetic characterization of Chinese rubella virus isolates from 2003 to 2007].
57 rubella virus strains were isolated using Vero cell line or Vero/SLAM cell line from patients' throat swabs during rubella outbreaks and sporadics in 10 provinces of China from 2003 to 2007. Fragments of 1107 nucleotides of E1 genes of the isolates were amplified by RT-PCR, the PCR products were directly sequenced and analyzed. The phylogenetic analysis based on 739 nucleotides showed that out of 57 Chinese rubella virus strains, 55 belong to a distinguish branch of 1E genotype when comparing with 1E genotype rubella strains from other countries, and the other 2 Chinese rubella virus strains belong to 2B genotype. Most of the nucleotide mutations of 57 rubella viruses were silent mutations, and the amino acid sequences were highly conserved. Except one amino acid change (Thr212 --> Ser212) in two rubella viruses at the hemagglutination inhibition and neutralization epitopes, there had no change found at the important antigenic epitope sites of the other rubella viruses. 1E genotype rubella viruses isolated from 10 provinces of China from 2003 to 2007, and two imported 2B genotype rubella viruses from Vietnam suggested that 1E genotype was the predominant genotype in this period of time. The rubella virus genotypes circulated during 2003 to 2007 were different from that circulating during 1979 to 1984 and 1999 to 2002, the rubella prevailed in recent years was mainly caused by 1E genotype rubella viruses with multi-transmission routes.
Zhu
Zhen
Z
Peking Union Medical College, Beijing 100730, China.
Xu
Wen-Bo
WB
Mao
Nai-Ying
NY
Jiang
Xiao-Hong
XH
Xu
Song-Tao
ST
He
Ji-Lan
JL
Sun
Li
L
Ling
Hua
H
Zhang
Zhen-Ying
ZY
Li
Cong-Yong
CY
Ba
Zhuo-Ma
ZM
Zhan
Jun
J
Chen
Hui
H
Wang
Fei-Xia
FX
Zhou
Shu-Jie
SJ
Chen
Xia
X
Zheng
Lei
L
Dai
De-Fang
DF
Zhang
Hong
H
Liang
Yong
Y
D005838:Genotype; D009154:Mutation; D010802:Phylogeny; D012412:Rubella virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013997:Time Factors