title: Decreased gene expression of human beta-defensin-1 in the development of squamous cell carcinoma of the oral cavity.
abstract: The aim of this study was to investigate the gene expression of human beta-defensin-1, -2, -3 (hBD-1, -2, -3), interleukin-1beta, tumour necrosis factor-alpha and cyclooxygenase-2 in oral squamous cell carcinoma (OSCC) compared to benign and premalignant lesions as well as healthy controls. Biopsies of healthy gingiva (n=5), irritation fibroma (n=5), leukoplakia (n=5) and OSCC (n=5) were obtained during routine surgical procedures. RNA was extracted according to standard protocols and transcripts of hBD-1, -2, -3, interleukin-1beta, tumour necrosis factor-alpha and cyclooxygenase-2 were analysed by real-time polymerase chain reaction. The expression of hBD-1 was reduced in all lesions (5-fold in irritation fibroma and 2.5-fold in leukoplakia), but most significantly (50-fold) in OSCC. hBD-1 appears to play a role in the development of OSCC. The loss of its function might contribute to the malignant progression of these tumours.
authors.affiliation: University of Bonn, Uni-Klinikum Bonn Klinik and Poliklinik for MKG-Chirurgie, Bonn, Germany. m.wenghoefer@gmx.de
authors.forename: M
authors.initials: M
authors.lastname: Wenghoefer
authors.forename: A
authors.initials: A
authors.lastname: Pantelis
authors.forename: H
authors.initials: H
authors.lastname: Dommisch
authors.forename: R
authors.initials: R
authors.lastname: Reich
authors.forename: M
authors.initials: M
authors.lastname: Martini
authors.forename: J-P
authors.initials: JP
authors.lastname: Allam
authors.forename: N
authors.initials: N
authors.lastname: Novak
authors.forename: S
authors.initials: S
authors.lastname: Bergé
authors.forename: S
authors.initials: S
authors.lastname: Jepsen
authors.forename: J
authors.initials: J
authors.lastname: Winter
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000032:analysis; D018450:Disease Progression; D005350:Fibroma / Q000235:genetics / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005881:Gingiva / Q000033:anatomy & histology; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D007972:Leukoplakia, Oral / Q000235:genetics / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D023083:beta-Defensins / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; D051546:Cyclooxygenase 2