[Determination of cinnamic acid in rat plasma after oral administration of subing orally disintegrating tablets and study of its pharmacokinetics behavior].
OBJECTIVE
To develop a RP-HPLC method for determination of cinnamic acid in rat plasma.


METHODS
The plasma samples were acidified with acetic acid and extracted with chloroform. Cinnamic acid was separated on a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) eluted with a mobile phase of methanol-acetonitrile-water-glacial acetic acid (25:20:55:0.3) at a flow rate of 1.0 mL x min(-1) and room temperature with UV detection at 278 nm, carbamazepine as internal standard.


RESULTS
The standard curve was linear over the range of 4.0 to approximately 400 ng x mL (-1) r = 0..99 9. The LOQ was 4.0 ng x mL(-1), the mean extraction recovery of the spiked samples at low, middle and high levels was 86.4%, while the mean method recovery was 100.3%. The RSD of intra-day and inter-day were both less than 6.0%.


CONCLUSIONS
The method was sensitive, specific, accurate and precise, which was used to study the pharmacokinetic profile of cinnamic acid in rat plasma after oral administration of the Subing orally disintegrating tablets.
Li
Pei-Fan
PF
College of Pharmaceuticals & Biotechnology Tianjin University, Tianjin 300072, China.
Zhang
Yun-Hui
YH
Xiao
Li
L
Jin
Xing-Hua
XH
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002934:Cinnamates / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics; D031317:Liquidambar / Q000737:chemistry; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013607:Tablets
D002934:Cinnamates; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D013607:Tablets; C029010:cinnamic acid