The effects of methylprednisolone and mitoxantrone on CCL5-induced migration of lymphocytes in multiple sclerosis.
OBJECTIVE
Chemokines are involved in migration of inflammatory cells to the central nervous system (CNS) in multiple sclerosis (MS). The aim of this study was the analysis of the impact of MS treatment on CCL5-induced migration of leukocyte subpopulations.


METHODS
Migration of lymphocytes and monocytes from blood of MS patients treated with methylprednisolone (MP) or mitoxantrone (MTX) was analysed in a chemotaxis chamber.


RESULTS
CCL5-induced migration of lymphocytes from untreated MS patients was significantly increased over controls. The treatment of MS with MP and MTX reduced this chemotaxis. The plasma level of CCL5 was increased in MS patients before treatment and was also significantly decreased in the treatment of MS with MP and MTX.


CONCLUSIONS
This observation supports the hypothesis that in MS, chemokine CCL5 may induce migration of leukocytes to the CNS and suggests that treatment of the disease with MP and MTX may reduce this migration.
Jalosinski
M
M
Department of Experimental and Clinical Neurology, Medical University of Lodz, Lodz, Poland.
Karolczak
K
K
Mazurek
A
A
Glabinski
A
A
D000700:Analgesics / Q000494:pharmacology; D002490:Central Nervous System / Q000166:cytology / Q000276:immunology; D018946:Chemokine CCL5 / Q000097:blood / Q000235:genetics / Q000276:immunology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000276:immunology; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008775:Methylprednisolone / Q000494:pharmacology*; D008942:Mitoxantrone / Q000494:pharmacology*; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology* / Q000378:metabolism
D000700:Analgesics; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D005938:Glucocorticoids; D008942:Mitoxantrone; D008775:Methylprednisolone