Enhanced efficiency of a capillary-based biosensor over an optical fiber biosensor for detecting calpastatin.
A capillary-based optical biosensor has been developed to detect calpastatin, an indicator of meat tenderness. Longissimus muscle samples (n=11) were extracted from beef carcasses at 0 and 48h post-mortem. These samples were assayed for calpastatin by traditional laboratory methods and with a newly developed capillary tube biosensor as well as for Warner-Bratzler shear force (WBSF) and crude protein and the responses were compared. Additionally, the response from the capillary-based biosensor was compared to a previously developed optical fiber biosensor. When the 0 and 48h sampling periods were combined, the capillary tube biosensor was moderately accurate in predicting calpastatin activity (R(2)=0.6058). There was less variation in the 0h capillary tube biosensor compared to the 0h pre-column (P=0.006) and post-column optical fiber biosensors (P=0.047), therefore the capillary tube biosensor is a more precise system of measurement. This research further advances the development of a calpastatin biosensor and makes online assessment one step closer to reality.
Bratcher
C L
CL
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
Grant
S A
SA
Vassalli
J T
JT
Lorenzen
C L
CL
D015374:Biosensing Techniques / Q000295:instrumentation*; D002135:Calcium-Binding Proteins / Q000008:administration & dosage*; D002197:Capillary Action; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005504:Food Analysis / Q000295:instrumentation*; D008460:Meat / Q000032:analysis*; D055100:Optical Fibers; D012680:Sensitivity and Specificity
D002135:Calcium-Binding Proteins; C033668:calpastatin