title: [Synergistic interactions of TRAIL and paclitaxel on the laryngeal squamous carcinoma cell line in vitro].
abstract: OBJECTIVE
  To investigate the synergistic cytotoxicity of TRAIL and paclitaxel on laryngeal squamous carcinoma cell line Hep-2.
  
  
  METHODS
  Hep-2 cells were exposed to various concentrations of TRAIL and paclitaxel respectively or jointly. The inhibition rate was measured by CCK-8 assay. The apoptosis rate and the expressions of DR4, DR5, DcR1 and DcR2 in Hep-2 cells were detected by flow cytometry methods.
  
  
  RESULTS
  Hep-2 cells were resistant to apoptosis induced by TRAIL. Paclitaxel could enhance its sensitivity to TRAIL by up-regulating the expressions of TRAIL death receptors in Hep-2 cells.
  
  
  CONCLUSIONS
  The resistance of Hep-2 cell to TRAIL could be overcome by paclitaxel. TRAIL and paclitaxel have synergistic killing effects on Hep-2 cells, so they might have a promising prospect in clinical therapy of laryngeal squamous carcinoma.
authors.affiliation: Department of Otolaryngology, Eye Ear Nose Throat Hospital, Fudan University, Shanghai, 200031, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy; D045744:Cell Line, Tumor; D004357:Drug Synergism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy; D017239:Paclitaxel / Q000494:pharmacology*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D017239:Paclitaxel