title: A novel Treponema pallidum antigen, TP0136, is an outer membrane protein that binds human fibronectin.
abstract: The antigenicity, structural location, and function of the predicted lipoprotein TP0136 of Treponema pallidum subsp. pallidum were investigated based on previous screening studies indicating that anti-TP0136 antibodies are present in the sera of syphilis patients and experimentally infected rabbits. Recombinant TP0136 (rTP0136) protein was purified and shown to be strongly antigenic during human and experimental rabbit infection. The TP0136 protein was exposed on the surface of the bacterial outer membrane and bound to the host extracellular matrix glycoproteins fibronectin and laminin. In addition, the TP0136 open reading frame was shown to be highly polymorphic among T. pallidum subspecies and strains at the nucleotide and amino acid levels. Finally, the ability of rTP0136 protein to act as a protective antigen to subsequent challenge with infectious T. pallidum in the rabbit model of infection was assessed. Immunization with rTP0136 delayed ulceration but did not prevent infection or the formation of lesions. These results demonstrate that TP0136 is expressed on the outer membrane of the treponeme during infection and may be involved in attachment to host extracellular matrix components.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
authors.forename: Mary Beth
authors.initials: MB
authors.lastname: Brinkman
authors.forename: Melanie A
authors.initials: MA
authors.lastname: McGill
authors.forename: Jonas
authors.initials: J
authors.lastname: Pettersson
authors.forename: Arthur
authors.initials: A
authors.lastname: Rogers
authors.forename: Petra
authors.initials: P
authors.lastname: Matejková
authors.forename: David
authors.initials: D
authors.lastname: Smajs
authors.forename: George M
authors.initials: GM
authors.lastname: Weinstock
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Norris
authors.forename: Timothy
authors.initials: T
authors.lastname: Palzkill
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D001428:Bacterial Vaccines; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011485:Protein Binding; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014210:Treponema pallidum / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D014211:Treponemal Infections / Q000276:immunology / Q000517:prevention & control; D022223:Vaccines, Subunit / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D005353:Fibronectins; D007797:Laminin; D011994:Recombinant Proteins; D022223:Vaccines, Subunit