title: Pathological changes in masked palm civets experimentally infected by severe acute respiratory syndrome (SARS) coronavirus.
abstract: Masked palm civets are highly susceptible to infection with the severe acute respiratory syndrome coronavirus (SARS-CoV). Infected animals become less aggressive and develop pyrexia, lethargy and diarrhoea. The present study describes the spectrum of histopathological changes in the lung, spleen, lymph node, liver, small intestine, kidney and cerebrum of civets infected experimentally with SARS-CoV. In-situ hybridization (ISH) with probes specific for the RNA polymerase gene demonstrated viral RNA in the lung, small intestine and cerebrum only. In-situ labelling was employed in order to demonstrate cellular apoptosis in the cerebrum, but there was no evidence of apoptosis within the myocardium. These results indicate that SARS-CoV causes multi-organ pathology in civets, similar to that observed in human SARS patients. These parallels suggest that civets may be used as an animal model of this infection to gain insight into the pathogenesis of SARS and for evaluation of candidate vaccines and antiviral drugs.
authors.affiliation: National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xiao
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.forename: X
authors.initials: X
authors.lastname: Yin
authors.forename: Y
authors.initials: Y
authors.lastname: Guan
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Tong
authors.forename: L-F
authors.initials: LF
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Kong
authors.forename: D
authors.initials: D
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002371:Cat Diseases / Q000473:pathology* / Q000821:virology; D002415:Cats; D054022:Cerebrum / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007421:Intestine, Small / Q000473:pathology / Q000821:virology; D007668:Kidney / Q000473:pathology / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D045473:Severe acute respiratory syndrome-related coronavirus / Q000302:isolation & purification / Q000472:pathogenicity*; D045169:Severe Acute Respiratory Syndrome / Q000473:pathology* / Q000821:virology; D013154:Spleen / Q000473:pathology / Q000821:virology; D045949:Viverridae
chemical_list: D012367:RNA, Viral