title: Mapping of the chromosome 17 BMD QTL in the F(2) male mice of MRL/MpJ x SJL/J.
abstract: Developing treatment strategies for osteoporosis would be facilitated by identifying genes regulating bone mineral density (BMD). One way to do so is through quantitative trait locus (QTL) mapping. However, there are sex differences in terms of the presence/absence and locations of BMD QTLs. In a previous study, our group identified a BMD QTL on chromosome 17 in the F(2) female mice of the MRL/MpJ x SJL/J cross. Here, we determined whether it was also present in the male mice of the same cross. Furthermore, we also intended to reduce the QTL region by increasing marker density. Interval mapping showed that the same QTL based on chromosomal positions was present in the male mice, with logarithmic odds (LOD) scores of 4.0 for femur BMD and 5.2 for total body BMD. Although there was a body weight QTL at the same location, the BMD QTL was not affected by the adjustment for body weight. Mapping with increased marker density indicated a most likely region of 35-55 Mb for this QTL. There were also co-localized QTLs for femur length, femur periosteal circumference (PC) and total body bone area, suggesting possibility of pleiotropy. Runx2 and VEGFA are strong candidate genes located within this QTL region.
authors.affiliation: Musculoskeletal Disease Center, Jerry L. Pettis Memorial VA Medical Center, Loma Linda, CA 92357, USA.
authors.forename: Hongrun
authors.initials: H
authors.lastname: Yu
authors.forename: Bouchra
authors.initials: B
authors.lastname: Edderkaoui
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cortez
authors.forename: Heather M
authors.initials: HM
authors.lastname: Davidson
authors.forename: Jon E
authors.initials: JE
authors.lastname: Wergedal
authors.forename: David J
authors.initials: DJ
authors.lastname: Baylink
authors.forename: Subburaman
authors.initials: S
authors.lastname: Mohan
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001835:Body Weight / Q000502:physiology; D015519:Bone Density / Q000235:genetics*; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D050659:Core Binding Factor Alpha 1 Subunit / Q000502:physiology; D003433:Crosses, Genetic; D005260:Female; D005269:Femur / Q000502:physiology; D006801:Humans; D008126:Lod Score; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010024:Osteoporosis / Q000235:genetics; D040641:Quantitative Trait Loci; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D015415:Biomarkers; D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse