Long-lasting in vivo gene silencing by electrotransfer of shRNA expressing plasmid.
RNA interference appears as a promising tool for therapeutic gene silencing. A key limit is the delivery of the siRNA. A safe approach is to use a physical method such as in vivo electropulsation with contact electrodes. Getting a long lived silencing can be better approached by using the in situ expression of shRNA. This is presently obtained by using co-electrotransfer of specific plasmids coding for expression and silencing of a fluorescent protein. Using a non invasive fluorescence imaging assay, electrodelivery in mouse muscles is observed to induce complete silencing over more than two months in a specific way. The proper choices of the plasmids (sequence and relative amounts) and of the electric pulsing conditions appear as key parameters in the successful silencing.
Escoffre
Jean-Michel
JM
IPBS Universite P Sabatier/CNRS, UMR 5089, 205 route de Narbonne, 31077 Toulouse, France.
Debin
Arnaud
A
Reynes
Jean-Paul
JP
Drocourt
Daniel
D
Tiraby
Gérard
G
Hellaudais
Laëtitia
L
Teissie
Justin
J
Golzio
Muriel
M
D000818:Animals; D018274:Electroporation / Q000379:methods*; D005260:Female; D020868:Gene Silencing; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering