Exogenous leptin promotes the recovery of regressed ovary in fasted ducks.
The present study was undertaken to examine the effect of administered recombinant mouse leptin on the recovery of regressed ovary in fasted ducks. Twenty-eight ducks were divided into five groups: fed ad libitum (control; n=5), fasted control (FC; n=5), fasted+low dose of leptin (F+L; n=5), fasted+medium dose of leptin (F+M; n=5) and fasted+high dose of leptin (F+H; n=3). All four fasted groups were fasted for 2 days and then ad libitum and the ducks were treated with leptin at doses of 0 (control and FC), 50 (F+L), 250 (F+M) and 1000 (F+H) microg/kg body weight/day on day 3-5. Results showed that a moderate dose of leptin (250 microg/kg body weight/day) injected during the re-feeding period: (i) promoted the recovery of the regressed ovary as evidenced by an increase in ovary weight and recovery of yellow hierarchical follicles; (ii) elevated the plasma 17beta-estradiol (E(2)) level; (iii) increased the mRNA levels of ovary follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and estrogen receptor-beta (ER-beta). Furthermore, the results also showed that a high dose of leptin (1000 microg/kg body weight/day) may have a negative effect on the recovery of the regressed ovary. In conclusion, this study indicates that, in ducks, leptin may be involved in the recovery of the regressed ovary caused by 2 days of fasting. This effect may be related to increased plasma E(2) levels and stimulation of the mRNA levels of ovarian FSHR, LHR and especially ER-beta.
Song
Yueqiang
Y
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China.
Wang
Chonggang
C
Wang
Cheng
C
Lv
Liangju
L
Chen
Yixin
Y
Zuo
Zhenghong
Z
D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D004372:Ducks / Q000235:genetics / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D047629:Estrogen Receptor beta / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D020738:Leptin / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D010058:Oviposition / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011962:Receptors, FSH / Q000096:biosynthesis / Q000235:genetics; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
D047629:Estrogen Receptor beta; D020738:Leptin; D012333:RNA, Messenger; D011962:Receptors, FSH; D011974:Receptors, LH; D011994:Recombinant Proteins; D004958:Estradiol