Poly(methylmethacrylate) microchip electrophoresis of proteins using linear-poly(acrylamide) solutions as separation matrix.
Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model.
Okada
Hiroki
H
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya, Japan. h051102d@mbox.nagoya-u.ac.jp
Kaji
Noritada
N
Tokeshi
Manabu
M
Baba
Yoshinobu
Y
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D046209:Electrophoresis, Microchip / Q000379:methods*; D008747:Methylcellulose / Q000737:chemistry; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D010316:Particle Size; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000032:analysis; D012996:Solutions / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D000099166:Polyacrylamides
D000180:Acrylic Resins; D008747:Methylcellulose; D019904:Polymethyl Methacrylate; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D000099166:Polyacrylamides