Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from Cannabis sativa.
Polyketide synthase-1 (PKS-1) is a novel type III polyketide synthase that catalyzes the biosynthesis of hexanoyl triacetic acid lactone in Cannabis sativa (Mexican strain). PKS-1 was overproduced in Escherichia coli, purified and finally crystallized in two different space groups. The crystal obtained in 0.1 M HEPES buffer pH 7.5 containing 0.2 M calcium acetate and 20%(w/v) polyethylene glycol 3350 diffracted to 1.65 A resolution and belonged to space group P1, with unit-cell parameters a = 54.3, b = 59.3, c = 62.6 A, alpha = 69, beta = 81, gamma = 80 degrees. Another crystal obtained in 0.1 M HEPES buffer pH 7.5 containing 0.2 M sodium chloride and 20%(w/v) polyethylene glycol 3350 diffracted to 1.55 A resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 54.3, b = 110, c = 130 A. These data will enable us to determine the crystal structure of PKS-1.
Taguchi
Chiho
C
Faculty of Pharmaceutical Sciences, Kyushu University, Japan.
Taura
Futoshi
F
Tamada
Taro
T
Shoyama
Yoshinari
Y
Shoyama
Yukihiro
Y
Tanaka
Hiroyuki
H
Kuroki
Ryota
R
Morimoto
Satoshi
S
D002188:Cannabis / Q000201:enzymology* / Q000235:genetics; D002384:Catalysis; D003460:Crystallization; D015394:Molecular Structure; D048630:Polyketide Synthases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011753:Pyrones / Q000737:chemistry / Q000378:metabolism; D014961:X-Ray Diffraction
D011753:Pyrones; C030720:triacetic acid lactone; D048630:Polyketide Synthases