title: Contribution of P2Y(1) receptors to ADP signalling in mouse spinal cord cultures.
abstract: Mixed neuronal and glial cell spinal cord cultures from neonates express ADP sensitive P2Y(1,12&13) receptors. ADP (10microM) evoked increases in intracellular calcium that were essentially abolished by the P2Y(1) receptor antagonist MRS2179 (10microM), responses were also absent in preparations from P2Y(1) receptor deficient mice however UTP (100microM) evoked calcium rises were unaffected. ADP also evoked a robust increase in extracellular signal-regulated protein kinase (ERK) phosphorylation that was of similar magnitude in the cultures from wild type and P2Y(1) receptor deficient mice. These results suggest that ADP acts through P2Y(1) receptors to mediate an increase in intracellular calcium but not to stimulate ERK phosphorylation in the spinal cord.
authors.affiliation: Department of Cell Physiology & Pharmacology, Henry Wellcome Building, University of Leicester, Leicester LE1 9HN, UK.
authors.forename: Amelia E
authors.initials: AE
authors.lastname: Atterbury-Thomas
authors.forename: Catherine
authors.initials: C
authors.lastname: Leon
authors.forename: Christian
authors.initials: C
authors.lastname: Gachet
authors.forename: Ian D
authors.initials: ID
authors.lastname: Forsythe
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Evans
mesh_terms: D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2 / Q000172:deficiency / Q000502:physiology*; D058472:Receptors, Purinergic P2Y1; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D046509:Tissue Culture Techniques; D014544:Uridine Triphosphate / Q000494:pharmacology; D014966:Xanthenes / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; C111914:N(6)-methyl-2'-deoxyadenosine 3',5'-diphosphate; C546383:P2ry1 protein, mouse; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D058472:Receptors, Purinergic P2Y1; D014966:Xanthenes; C059715:Fluo-3; D000244:Adenosine Diphosphate; D002118:Calcium; D014544:Uridine Triphosphate