Optimization of specimen preparation of thin cell section for AFM observation.
High resolution imaging of intracellular structures of ultrathin cell section samples is critical to the performance of precise manipulation by atomic force microscopy (AFM). Here, we test the effect of multiple factors during section sample preparation on the quality of the AFM image. These factors include the embedding materials, the annealing process of the specimen block, section thickness, and section side. We found that neither the embedding materials nor the temperature and speed of the annealing process has any effect on AFM image resolution. However, the section thickness and section side significantly affect the surface topography and AFM image resolution. By systematically testing the image quality of both sides of cell sections over a wide range of thickness (40-1000 nm), we found that the best resolution was obtained with upper-side sections approximately 50-100 nm thick. With these samples, we could observe precise structure details of the cell, including its membrane, nucleoli, and other organelles. Similar results were obtained for other cell types, including Tca8113, C6, and ECV-304. In brief, by optimizing the condition of ultrathin cell section preparation, we were able to obtain high resolution intracellular AFM images, which provide an essential basis for further AFM manipulation.
Li
Xinhui
X
Nanobiology Laboratory, Bio-X Life Science Research Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Minhang District, Shanghai 200240, China.
Ji
Tong
T
Hu
Jun
J
Sun
Jielin
J
D000818:Animals; D002460:Cell Line; D004853:Epoxy Resins; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006358:Hot Temperature; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods*; D008867:Microtomy / Q000379:methods; D016613:Plastic Embedding / Q000379:methods; D051381:Rats
D004853:Epoxy Resins