[Microarray-based methods to identify DNA methylation in cancer].
DNA methylation is an epigenetic modification associated with gene transcription regulation, X-chromosome inactivation, regulation of development and cell differentiation. Aberrant DNA methylation has been linked to cancer. The advent of microarray technology has provided new opportunities for high-throughput study on DNA methylation. Various microarray-based methods depend on different pretreatments including immunoprecipitation and restriction digestion. Al-though each technique has its limitations, the immunoprecipitation method has high specificity, and the restriction digestion method shows high sensitivity. Together they provide many choices to the study of genome-wide DNA methylation profile in cancer.
Tian
Xiao-Qing
XQ
Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China. txqwl2004@yahoo.com.cn
Sun
Dan-Feng
DF
Zhang
Yan-Jie
YJ
Fang
Jing-Yuan
JY
D000818:Animals; D019175:DNA Methylation / Q000235:genetics*; D006801:Humans; D008957:Models, Genetic; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*