title: Fine-tuned expression of programmed death 1 ligands in mature dendritic cells stimulated by CD40 ligand is critical for the induction of an efficient tumor specific immune response.
abstract: During maturation, murine myeloid dendritic cells (DCs) upregulated the expressions of CD11c, CD25, CD40, CD80, CD86, MHC II and programmed death 1 ligands 1 and 2 (PD-L1 and PD-L2). Differential expression patterns of PD-L1 and PD-L2 were found when DCs were triggered by CD40 ligand and TNF-alpha. PD-L1 expression was repressed and PD-L2 expression remained unchanged in mature CD40-ligated DCs, whereas TNF-alpha stimulated DCs kept high expression of PD-L1 and significantly enhanced PD-L2 expression on DCs. Proliferations of T lymphocytes stimulated by immature DCs were enhanced by blockade of the PD-1 and PD-1 ligand interaction. But inhibitive effects were found in T lymphocytes stimulated by CD40-ligated DCs. With the fine-tuned expressions of PD-L1 and PD-L2, CD40-ligated DCs could sustain a longer activation period and elicit a more efficient T lymphocyte activation.
authors.affiliation: Medical Biotechnology Institute and Clinical Immunology Laboratory for Jiangsu Province, Soochow University, Suzhou, Jiangsu 215007, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Gu
authors.forename: Yi Bei
authors.initials: YB
authors.lastname: Zhu
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yong Jing
authors.initials: YJ
authors.lastname: Chen
authors.forename: Ge Hua
authors.initials: GH
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Ge
authors.forename: Shi Yong
authors.initials: SY
authors.lastname: Zhou
authors.forename: Huan
authors.initials: H
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Yu Hua
authors.initials: YH
authors.lastname: Qiu
authors.forename: Xue Guang
authors.initials: XG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D018122:B7-1 Antigen / Q000276:immunology / Q000378:metabolism*; D060890:B7-H1 Antigen; D023201:CD40 Ligand / Q000276:immunology / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D008024:Ligands; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000276:immunology*; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D061027:Programmed Cell Death 1 Ligand 2 Protein; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; D008024:Ligands; D008562:Membrane Glycoproteins; C556978:Pdcd1lg2 protein, mouse; D010455:Peptides; D061027:Programmed Cell Death 1 Ligand 2 Protein; D014409:Tumor Necrosis Factor-alpha; D023201:CD40 Ligand