Expression change of beta-1,4 galactosyltransferase I, V mRNAs and Galbeta1,4GlcNAc group in rat sciatic nerve after crush.
Glycosylation is one of the most important post-translational modifications. It is clear that the single step of beta-1,4-galactosylation is performed by a family of beta-1,4-galactosyltransferases (beta-1,4-GalTs), and that each member of this family may play a distinct role in different tissues and cells. beta-1,4-GalT I and V are involved in the biosynthesis of N-linked oligosaccharides. In the present study, Real-time PCR revealed that the beta-1,4-GalT I and V mRNAs reached peaks at 2 w after sciatic nerve crush. In situ hybridization showed that at 1 d after sciatic nerve crush, the expression levels of beta-1,4-GalT I and V mRNAs were strong at the crush site, and decreased gradually from crush site to the distal segments. In addition, combined in situ hybridization for beta1,4-GalT I and V mRNAs and immunohistochemistry for S100 showed that beta1,4-GalT I and V mRNAs were mainly located in Schwann cells. Lectin blot showed that the expression of Galbeta1,4GlcNAc group increased at 6 h immediately, reached a peak at 12 h and remained elevated up to 4 w after sciatic nerve crush. In conclusion, beta1,4-GalT I and V might play important roles in the regeneration of the injured sciatic nerve, and upregulation of Galbeta1,4GlcNAc group might be correlated with the process of the sciatic nerve injury.
Yan
Meijuan
M
Laboratory of Aging and Nervous Diseases & Cytoneurobiology Unit, Soochow University, Suzhou, 215123, PR China.
Cheng
Chun
C
Shao
Xiaoyi
X
Qian
Ji
J
Shen
Aiguo
A
Xia
Chunlin
C
Xia
Chun
C
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D005700:Galactosyltransferases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006023:Glycoproteins / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D037102:Lectins / Q000378:metabolism; D023421:Models, Animal; D009409:Nerve Crush; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012584:Sciatic Nerve / Q000201:enzymology* / Q000473:pathology*; D013997:Time Factors
D006023:Glycoproteins; D037102:Lectins; D012333:RNA, Messenger; D005700:Galactosyltransferases; C119701:beta-1,4-galactosyltransferase I; C419408:beta-1,4-galactosyltransferase V; D000117:Acetylglucosamine