title: Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli.
abstract: Naturally invasive bacteria have been successfully used for mucosal delivery of DNA vaccines against bacterial, viral and tumour antigens. Recently, an alternative delivery system based on a genetically modified mutant of the non-pathogenic commensal bacterium Escherichia coli, was developed and successfully used to deliver therapeutic genes and immunogenic proteins to epithelial cells in vivo. In this work, we used these recombinant invasive bacteria to deliver DNA vaccines against two Mycobacterium tuberculosis proteins (FbpA, and HtpX) following intranasal administration. Both DNA vaccines were able to induce an antigen-specific T-cell response. Moreover, mice immunized with the recombinant bacteria carrying the DNA vaccine encoding HtpX, were significatively protected from challenge with M. tuberculosis.
authors.affiliation: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, 35121 Padua, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Brun
authors.forename: Antonella
authors.initials: A
authors.lastname: Zumbo
authors.forename: Ignazio
authors.initials: I
authors.lastname: Castagliuolo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Delogu
authors.forename: Filippo
authors.initials: F
authors.lastname: Manfrin
authors.forename: Michela
authors.initials: M
authors.lastname: Sali
authors.forename: Giovanni
authors.initials: G
authors.lastname: Fadda
authors.forename: Catherine
authors.initials: C
authors.lastname: Grillot-Courvalin
authors.forename: Giorgio
authors.initials: G
authors.lastname: Palù
authors.forename: Riccardo
authors.initials: R
authors.lastname: Manganelli
mesh_terms: D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics; D000281:Administration, Intranasal; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D007115:Immunization Schedule; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D006360:Heat-Shock Proteins; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis