title: A semi-defined medium without serum for small ruminant mycoplasmas.
abstract: The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri.
authors.affiliation: Unidad de Epidemiología y Medicina Preventiva, Spain. aramirez@dpat.ulpgc.es
authors.forename: Ana S
authors.initials: AS
authors.lastname: Ramírez
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Fleitas
authors.forename: Rubén S
authors.initials: RS
authors.lastname: Rosales
authors.forename: Carlos
authors.initials: C
authors.lastname: Poveda
authors.forename: Christian
authors.initials: C
authors.lastname: de la Fe
authors.forename: Marisa
authors.initials: M
authors.lastname: Andrada
authors.forename: Ayoze
authors.initials: A
authors.lastname: Castro
authors.forename: José B
authors.initials: JB
authors.lastname: Poveda
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D016895:Culture Media, Serum-Free / Q000737:chemistry*; D009174:Mycoplasma / Q000302:isolation & purification*; D012418:Ruminants / Q000382:microbiology*
chemical_list: D016895:Culture Media, Serum-Free