Simultaneous determination of calycosin-7-O-beta-D-glucoside, ononin, astragaloside IV, astragaloside I and ferulic acid in rat plasma after oral administration of Danggui Buxue Tang extract for their pharmacokinetic studies by liquid chromatography-mass spectrometry.
A sensitive and reliable high-performance liquid chromatography-mass spectrometry (HPLC-MS) was developed and validated for simultaneous quantification of five main bioactive components, i.e., calycosin-7-O-beta-D-glucoside, ononin, astragaloside IV, astragaloside I and ferulic acid in rat plasma after oral administration of Danggui Buxue Tang (DBT) extract. Plasma samples were extracted with solid-phase extraction (SPE) separated on an Inertsil ZORBAX C(18) column and detected by MS with electrospray ionization (ESI) interface in negative selective ion monitoring (SIM) mode. Calibration curves offered linear ranges of two orders of magnitude with r(2)>0.99. The method had the lower limit quantification of 0.55, 0.46, 1.07, 1.12 and 4.6 ng/mL for calycosin-7-O-beta-D-glucoside, ononin, astragaloside IV, astragaloside I and ferulic acid, respectively, with precision less than 10%. The RSD of intra- and inter-day variations ranged from 2.10% to 6.19% and 2.37% to 6.72%. This developed method was subsequently applied to pharmacokinetic studies of the five compounds in rats successfully.
Wen
Xiao-Dong
XD
Key Laboratory of Modern Chinese Medicines, Ministry of Education, Department of Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
Qi
Lian-Wen
LW
Li
Ping
P
Bao
Kang-De
KD
Yan
Xiao-Wei
XW
Yi
Ling
L
Li
Chang-Yin
CY
D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000097:blood*; D004365:Drugs, Chinese Herbal; D005960:Glucosides / Q000097:blood*; D007529:Isoflavones / Q000097:blood*; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014315:Triterpenes / Q000097:blood*
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; C526426:calycosin-7-O-beta-D-glucoside; C477279:danggui buxue decoction; C052064:astragaloside A; C004999:ferulic acid