title: Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE).
abstract: One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.
authors.affiliation: Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103, USA.
authors.forename: Eric W
authors.initials: EW
authors.lastname: Deutsch
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Ball
authors.forename: Jules J
authors.initials: JJ
authors.lastname: Berman
authors.forename: G Steven
authors.initials: GS
authors.lastname: Bova
authors.forename: Alvis
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authors.lastname: Brazma
authors.forename: Roger E
authors.initials: RE
authors.lastname: Bumgarner
authors.forename: David
authors.initials: D
authors.lastname: Campbell
authors.forename: Helen C
authors.initials: HC
authors.lastname: Causton
authors.forename: Jeffrey H
authors.initials: JH
authors.lastname: Christiansen
authors.forename: Fabrice
authors.initials: F
authors.lastname: Daian
authors.forename: Delphine
authors.initials: D
authors.lastname: Dauga
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Davidson
authors.forename: Gregory
authors.initials: G
authors.lastname: Gimenez
authors.forename: Young Ah
authors.initials: YA
authors.lastname: Goo
authors.forename: Sean
authors.initials: S
authors.lastname: Grimmond
authors.forename: Thorsten
authors.initials: T
authors.lastname: Henrich
authors.forename: Bernhard G
authors.initials: BG
authors.lastname: Herrmann
authors.forename: Michael H
authors.initials: MH
authors.lastname: Johnson
authors.forename: Martin
authors.initials: M
authors.lastname: Korb
authors.forename: Jason C
authors.initials: JC
authors.lastname: Mills
authors.forename: Asa J
authors.initials: AJ
authors.lastname: Oudes
authors.forename: Helen E
authors.initials: HE
authors.lastname: Parkinson
authors.forename: Laura E
authors.initials: LE
authors.lastname: Pascal
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pollet
authors.forename: John
authors.initials: J
authors.lastname: Quackenbush
authors.forename: Mirana
authors.initials: M
authors.lastname: Ramialison
authors.forename: Martin
authors.initials: M
authors.lastname: Ringwald
authors.forename: David
authors.initials: D
authors.lastname: Salgado
authors.forename: Susanna-Assunta
authors.initials: SA
authors.lastname: Sansone
authors.forename: Gavin
authors.initials: G
authors.lastname: Sherlock
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Stoeckert
authors.forename: Jason
authors.initials: J
authors.lastname: Swedlow
authors.forename: Ronald C
authors.initials: RC
authors.lastname: Taylor
authors.forename: Laura
authors.initials: L
authors.lastname: Walashek
authors.forename: Anthony
authors.initials: A
authors.lastname: Warford
authors.forename: David G
authors.initials: DG
authors.lastname: Wilkinson
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
authors.forename: Alvin Y
authors.initials: AY
authors.lastname: Liu
authors.forename: Lawrence D
authors.initials: LD
authors.lastname: True
mesh_terms: D019295:Computational Biology / Q000379:methods / Q000592:standards; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards*; D017403:In Situ Hybridization / Q000379:methods / Q000592:standards*