Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish.
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.
Langenau
D M
DM
Stem Cell Program and Division of Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, MA 2115, USA. dlangenau@enders.tch.harvard.edu
Keefe
M D
MD
Storer
N Y
NY
Jette
C A
CA
Smith
A C H
AC
Ceol
C J
CJ
Bourque
C
C
Look
A T
AT
Zon
L I
LI
D000818:Animals; D030801:Animals, Genetically Modified; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000528:radiation effects*; D002970:Cleavage Stage, Ovum; D004268:DNA-Binding Proteins / Q000008:administration & dosage / Q000235:genetics; D004625:Embryo, Nonmammalian; D018014:Gene Transfer Techniques; D019254:Genes, bcl-2; D016259:Genes, myc; D016158:Genes, p53; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000235:genetics; D008164:Luminescent Proteins / Q000008:administration & dosage / Q000235:genetics; D008845:Microinjections / Q000379:methods*; D050505:Mutant Proteins / Q000235:genetics; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D009687:Nuclear Proteins / Q000008:administration & dosage / Q000235:genetics; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D019076:Transgenes; D015027:Zebrafish / Q000196:embryology; D018631:ras Proteins / Q000235:genetics; D000097573:Red Fluorescent Protein
D004268:DNA-Binding Proteins; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D050505:Mutant Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins; D000097573:Red Fluorescent Protein; C117307:KRAS protein, human; C499311:RAG2 protein, human