Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus.
In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
Maekawa
Takaki
T
Institut für Genetik, Ludwig-Maximilians-Universität München, Maria-Ward-Str. 1a, 80638 München, Germany.
Kusakabe
Mitsumasa
M
Shimoda
Yoshikazu
Y
Sato
Shusei
S
Tabata
Satoshi
S
Murooka
Yoshikatsu
Y
Hayashi
Makoto
M
D017796:Caulimovirus / Q000235:genetics; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics*; D000070116:Lotus / Q000166:cytology / Q000235:genetics*; D008957:Models, Genetic; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics; D025821:Polyubiquitin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D053204:Root Nodules, Plant / Q000235:genetics
D025821:Polyubiquitin