[Research on method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel].
OBJECTIVE
To study a method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel and develop an assay correspondingly.


METHODS
After extracting the freeze dried gel with acetic ether, detected it in colorimetric method.


RESULTS
The interference of poloxamer was removed thoroughly, while the retention rate of the polysacchrarides showed to be 100.11% (n = 3). The average recovery was 102.6% (n = 6).


CONCLUSIONS
The method shows to be simple, maneuverable, stable and well reproducible.
Jiang
Ling-hai
LH
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Feng
Yi
Y
Shen
Lan
L
Xu
De-sheng
DS
D003124:Colorimetry / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005612:Freeze Drying; D005782:Gels; D010946:Plants, Medicinal / Q000737:chemistry; D020442:Poloxamer / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D031347:Prunella / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012995:Solubility; D013696:Temperature
D004365:Drugs, Chinese Herbal; D005782:Gels; D011134:Polysaccharides; D020442:Poloxamer