title: Correlation of autoantibodies presence detected by IFA-anti-dsDNA, IFA-AMA and immunoblotting with corresponding data in clinical management of autoimmune diseases.
abstract: Diagnosis and management of patients with SLE (Systemic Lupus Eritematosus), autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), involves specific diagnostic tests, such as IFA-AMA, IFA anti-dsDNA and immunoblotting for the detection of autoantibodies for specific autoantigens (mitochondria, dsDNA, M2, LKM-1, LC-1, SLA/LP). We established specific correlation between the detected autoantibodies and corresponding clinical findings. The total of 813 serum specimens were probed with IFA-anti-dsDNA, 98 of which tested positive. We also performed dilution analysis to the end point for all the positive specimens. Numerous specimens were tested by IFA, AMA and immunoblotting.
authors.affiliation: Institute of Clinical Immunology - University of Sarajevo Clinics Centre, Bolnicka 25, 71000 Sarajevo, Bosnia and Herzegovina.
authors.forename: Demo
authors.initials: D
authors.lastname: Subasić
authors.forename: Jasenko
authors.initials: J
authors.lastname: Karamehić
authors.forename: Farid
authors.initials: F
authors.lastname: Ljuca
authors.forename: Faris
authors.initials: F
authors.lastname: Gavrankapetanović
authors.forename: Marina
authors.initials: M
authors.lastname: Delić-Sarac
authors.forename: Izet
authors.initials: I
authors.lastname: Eminović
authors.forename: Dragan
authors.initials: D
authors.lastname: Kovacević
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D019693:Hepatitis, Autoimmune / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology*; D032389:Myocytes, Smooth Muscle; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology
chemical_list: D001323:Autoantibodies; D011768:Pyruvate Dehydrogenase Complex; C060729:anti-liver kidney microsome antibody; D004247:DNA