Preparation of high-purity fructo-oligosaccharides by Aspergillus japonicus beta-fructofuranosidase and successive cultivation with yeast.
The short-chain FOSs with high purity were prepared using a two-step strategy: Aspergillus japonicus extracellular beta-fructofuranosidase-catalyzed synthesis of FOSs followed by cultivation with Pichia pastoris (P. pastoris). The higher FOSs content was obtained after 8 h under the catalysis of beta-fructofuranosidase at pH 5.5 and 55 degrees C. Successive P. pastoris cultivation exhausts almost all monosugars in 12 h at 30 degrees C, which increases the purity of FOSs, and also recovers beta-fructofuranosidase activity by ceasing the inhibition of glucose from catalysis of the enzyme, yielding more FOSs. Finally, the FOSs purity was increased from 56.55 to 84.45% (26.47% 1-kestose and 57.98% nystose).
Yang
Ya-Lin
YL
Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie Street, Beijing 100081, People's Republic of China.
Wang
Jian-Hua
JH
Teng
Da
D
Zhang
Fan
F
D001230:Aspergillus / Q000201:enzymology*; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009844:Oligosaccharides / Q000096:biosynthesis*; D010843:Pichia / Q000378:metabolism*; D013395:Sucrose / Q000032:analysis; D043324:beta-Fructofuranosidase / Q000378:metabolism*
D009844:Oligosaccharides; C116580:fructooligosaccharide; D005632:Fructose; D013395:Sucrose; D043324:beta-Fructofuranosidase; D005947:Glucose