title: [Effect of anisomycin on allogeneic skin transplantation in mice].
abstract: OBJECTIVE
  To investigate the effect of anisomycin on the reactivity of lymphocytes and the rejection in the skin transplantation in mice.
  
  
  METHODS
  The reactivity of T cells in unilateral or bilateral mixed lymphocyte reaction was detected by MTT assay. The skins from C57BL/6 mice were transplanted to BALB/c mice and the survival period of the grafts was assessed. The recipients were divided into three groups (physiological saline group, cyclosporin A group and anisomycin group). They were injected with physiological saline (0.1 mL), cyclosporin A (20 mg/kg) and anisomycin (12.5 mg/kg) separately once a day for 3 days before the operation and 7 days after the operation.
  
  
  RESULTS
  With the optimal dose of 10.0 microg/L, anisomycin inhibited the reactivity of lymphocyte cells in both unilateral and bilateral mixed lymphocyte reactions. The survival period of the grafts from anisomycin group was longer than that from cyclosporin A group and physiological saline group.
  
  
  CONCLUSIONS
  Anisomycin can inhibit the reaction of lymphocytes and prolong the survival period of the grafts in skin transplantation effectively. It could be used as a new way for transplantation rejection treatment.
authors.affiliation: Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.forename: Fei-yue
authors.initials: FY
authors.lastname: Xing
authors.forename: Jing-fang
authors.initials: JF
authors.lastname: DI
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000627:therapeutic use*; D006084:Graft Rejection / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation / Q000379:methods*; D014184:Transplantation, Homologous / Q000379:methods*
chemical_list: D000841:Anisomycin