[Inhibitory effect of anti-Lewis y antibody on alpha1,2-fucosyltransferase gene transfected human ovarian cancer cells in vitro].
OBJECTIVE
To study the inhibitory effect of anti-Lewis y monoclonal antibody on proliferation, adhesion and invasion of alpha1, 2-fucosyltransferase gene transfected human ovarian cancer cell line RMG-I-H with over-expression of Lewis y antigen in vitro.


METHODS
The changes of proliferation, adhesion and invasion of RMG-I-H cells in vitro were detected by cell growth assay, cell adhesive assay and cell invasive assay, respectively, with goat anti-human IgG antibody treated group or non-antibody treated group as control.


RESULTS
The proliferation and adhesion of RMG-I-H cells were remarkably inhibited by preincubation of the tumor cells with anti-Lewis y antibody in vitro (P<0.05). The growth inhibitory rate from the second day to the seventh day was 5.62%, 19.75%, 34.96%, 46.51%, 49.78% and 33.33%, respectively. The adhesion inhibitory rate at different time points (15, 30, 60 min) was 47.59%, 58.64% and 13.85%, respectively. Invasive assay with a transwell cell culture chamber showed that there were no significant differences of invasion in vitro between each concentration of the Lewis y antibody treated group and non-antibody treated group (P>0.05).


CONCLUSIONS
Anti-Lewis y antibody significantly inhibits the proliferation and adhesion of RMG-I-H cells cultured in vitro, but it has no effect on invasion, which indicates that Lewis y is associated with some biological behaviors, such as proliferation and adhesion. The antibody against Lewis y may be an effective anti-metastasis agent for the treatment of patients with ovarian cancer.
Li
Fei-fei
FF
Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang 110004, China. feifei_li1982@hotmail.com
Lin
Bei
B
Hao
Ying-ying
YY
Liu
Juan-juan
JJ
Zhang
Fan
F
Zhang
Shu-lan
SL
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D014162:Transfection
D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D005647:Fucosyltransferases