title: MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity.
abstract: The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 microM at 50 degrees C. MJ0400-His(6) is inhibited competitively by erythrose-4-phosphate with a K(i) of 380 microM and displays heat stability with a half-life of c. 1 h at 100 degrees C. Hence, MJ0400 is the second gene encoding for an FBP aldolase in M. jannaschii. Previously, MJ0400 was shown to act as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase. This indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in M. jannaschii.
authors.affiliation: Institute of Microbiology, Universität Stuttgart, Stuttgart, Germany. anne.samland@imb.uni-stuttgard.de
authors.forename: Anne K
authors.initials: AK
authors.lastname: Samland
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Georg A
authors.initials: GA
authors.lastname: Sprenger
mesh_terms: D002244:Carbon / Q000378:metabolism; D003001:Cloning, Molecular; D004099:Dihydroxyacetone Phosphate; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D017015:Methanococcales / Q000201:enzymology* / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012765:Shikimic Acid / Q000378:metabolism; D013403:Sugar Phosphates / Q000494:pharmacology; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D013403:Sugar Phosphates; D005986:Glyceraldehyde 3-Phosphate; D012765:Shikimic Acid; D004099:Dihydroxyacetone Phosphate; C026959:erythrose 4-phosphate; D002244:Carbon; D005634:Fructose-Bisphosphate Aldolase