title: Budding yeast Mms22 and Mms1 regulate homologous recombination induced by replisome blockage.
abstract: Yeast cells lacking MMS22 or MMS1 are hypersensitive to agents that perturb replisome progression but the cellular functions of these genes are unknown. In this study we investigate the involvement of budding yeast MMS22 and MMS1 in homologous recombination (HR). Recombination between sister chromatids or between homologous chromosomes induced by agents that block replisomes was severely defective in cells lacking MMS22 or MMS1. In contrast, HR induced by double-strand breaks was not affected by the absence of these genes. Major defects in MMS-induced HR were also observed in cells lacking the cullin RTT101, the histone acetyltransferase RTT109 and in cells lacking the histone chaperone ASF1, all of which interact genetically with MMS22 and MMS1. Finally, we show that cells lacking either MMS22 or MMS1 are defective in recovery from MMS-induced replisome stalling. These results identify Mms22 and Mms1 as S-phase specific recombination-promoting factors.
authors.affiliation: MRC Protein Phosphorylation Unit, Sir James Black Centre, University of Dundee, Hawkhill, Dundee DD1 5EH, Scotland, UK.
authors.forename: Eris
authors.initials: E
authors.lastname: Duro
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Vaisica
authors.forename: Grant W
authors.initials: GW
authors.lastname: Brown
authors.forename: John
authors.initials: J
authors.lastname: Rouse
mesh_terms: D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D011995:Recombination, Genetic / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D000099272:Replisomes
chemical_list: D004271:DNA, Fungal; D004259:DNA-Directed DNA Polymerase; D008741:Methyl Methanesulfonate; D009097:Multienzyme Complexes; D009153:Mutagens; D019384:Nucleic Acid Synthesis Inhibitors; D029701:Saccharomyces cerevisiae Proteins; D000099272:Replisomes; C530190:Mms1 protein, S cerevisiae; C497133:Mms22 protein, S cerevisiae