title: Evaluation of liquid biphasic Granada medium and instant liquid biphasic Granada medium for group B streptococcus detection.
abstract: BACKGROUND
  Group B streptococci (GBS) are transmitted from the mother to the newborn. Prevention of neonatal infection is achieved by intrapartum prophylaxis given to mothers colonized with GBS at 35 to 37 weeks of pregnancy.
  
  
  METHODS
  Liquid biphasic Granada medium (LB) and instant liquid biphasic Granada medium (ILB) were evaluated for GBS detection. Vaginal swabs obtained from 300 women were inoculated onto LB or ILB, or onto Todd-Hewitt broth and analyzed with the ATB system (comparison method).
  
  
  RESULTS
  Prevalence of GBS was 20% (61/300). LB and Todd-Hewitt with ATB detected GBS in 20% of women, and ILB in 19% of women. No growth was observed at four hours in any of the media studied. At 10 h and 14 h, identification of GBS was possible in 43/300 (14%) and 53/300 (18%) of ILB cultures, respectively, and in 32/300 (11%) and 46/300 (15%) of LB cultures.
  
  
  CONCLUSIONS
  All the media used are suitable for GBS detection. The majority of GBS were identified in ILB and LB cultures at 10 h and 14 h.
authors.affiliation: Sexually Transmitted Diseases Unit, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: Filipa
authors.initials: F
authors.lastname: Martinho
authors.forename: Emília
authors.initials: E
authors.lastname: Prieto
authors.forename: Dulce
authors.initials: D
authors.lastname: Pinto
authors.forename: Rita Maria
authors.initials: RM
authors.lastname: Castro
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Morais
authors.forename: Luísa
authors.initials: L
authors.lastname: Salgado
authors.forename: Filomena da Luz
authors.initials: Fda L
authors.lastname: Exposto
mesh_terms: D000328:Adult; D003470:Culture Media / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D012007:Rectum / Q000382:microbiology; D013290:Streptococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control / Q000635:transmission; D013292:Streptococcus agalactiae / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D014621:Vagina / Q000382:microbiology
chemical_list: D003470:Culture Media