title: [Immunogenic properties of recombinant vaccinia virus containing the human IL-2 gene].
abstract: A genetic construct of the human interleukin-2 (IL-2) gene within vaccinia virus (L-IVP strain) has been designed. The authors show the capacity of CV-1 cells infected with the recombinant vaccinia virus VV-SIL2 to secrete human IL-2 into the culture medium. Human IL-2 has been detected by immunoblotting. The sera from the animals immunized with the recombinant virus VV-SIL2 exhibited both human IL-2 and its antibodies throughout the observation period. This recombinant virus immunization induced both humoral and cell-mediated immune responses to human IL-2; the observed changes in the concentrations of cytokines are likely to suggest that the response predominantly followed a Th1 pathway. The study construct was nontoxic at the used concentrations and administration routes. The findings point that it is promising to investigate the adjuvant properties of the recombinant VV-SIL2 vaccine-based preparation for immunization in combination with various vaccines and to study this construct in therapy for cancer diseases.
authors.forename: I V
authors.initials: IV
authors.lastname: Babkin
authors.forename: I A
authors.initials: IA
authors.lastname: Riazankin
authors.forename: L R
authors.initials: LR
authors.lastname: Lebedev
authors.forename: L E
authors.initials: LE
authors.lastname: Bulychev
authors.forename: A E
authors.initials: AE
authors.lastname: Nesterov
authors.forename: O V
authors.initials: OV
authors.lastname: Nosareva
authors.forename: I N
authors.initials: IN
authors.lastname: Babkina
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D016207:Cytokines / Q000097:blood; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D007279:Injections, Subcutaneous; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011213:Poxviridae Infections / Q000097:blood* / Q000276:immunology*; D012900:Smallpox Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D007376:Interleukin-2; D012900:Smallpox Vaccine; D014614:Vaccines, Synthetic