Therapeutic target metabolism observed using hyperpolarized 15N choline.
Choline is a precursor of cellular phospholipid metabolism that provides Magnetic Resonance (MR) and Positron Emission Tomography (PET) biomarkers for cancer detection and response assessment. Employing Dynamic Nuclear Polarization we show that the MR signal of 15N in choline can be enhanced by at least 4 orders of magnitude with a relaxation time of ca. 4 min, providing a method to observe the action of choline kinase, an important target for novel cancer therapeutics.
Gabellieri
Cristina
C
Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research, Royal Marsden NHS Foundation Trust, Downs Road Sutton, Surrey, SM2 5PT, United Kingdom.
Reynolds
Steven
S
Lavie
Arnon
A
Payne
Geoffrey S
GS
Leach
Martin O
MO
Eykyn
Thomas R
TR
D002794:Choline / Q000737:chemistry* / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism
D009587:Nitrogen Isotopes; D010767:Phosphorylcholine; D002797:Choline Kinase; D002794:Choline