title: Protein expression profile of Gluconacetobacter diazotrophicus PAL5, a sugarcane endophytic plant growth-promoting bacterium.
abstract: This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent approaches: 2-DE followed by MALDI-TOF or TOF-TOF MS and 1-DE followed by chromatography in a C18 column online coupled to an ESI-Q-TOF or ESI-IT mass spectrometer. The 583 identified proteins were sorted into functional categories and used to describe potential metabolic pathways for nucleotides, amino acids, carbohydrates, lipids, cofactors and energy production, according to the Enzyme Commission of Enzyme Nomenclature (EC) and Kyoto Encyclopedia of genes and genomes (KEGG) databases. The identification of such proteins and their possible insertion in conserved biochemical routes will allow comparisons between G. diazotrophicus and other bacterial species. Furthermore, the 88 proteins classified as conserved unknown or unknown constitute a potential target for functional genomic studies, aiming at the understanding of protein function and regulation of gene expression. The knowledge of metabolic fundamentals and coordination of these actions are crucial for the rational, safe and sustainable interference on crops. The entire dataset, including peptide sequence information, is available as Supporting Information and is the major contribution of this work.
authors.affiliation: Rio de Janeiro Proteomics Network, Rio de Janeiro, Brazil. leticialery@superig.com.br
authors.forename: Leticia M S
authors.initials: LM
authors.lastname: Lery
authors.forename: Ana
authors.initials: A
authors.lastname: Coelho
authors.forename: Wanda M A
authors.initials: WM
authors.lastname: von Kruger
authors.forename: Mayla S M
authors.initials: MS
authors.lastname: Gonçalves
authors.forename: Marise F
authors.initials: MF
authors.lastname: Santos
authors.forename: Richard H
authors.initials: RH
authors.lastname: Valente
authors.forename: Eidy O
authors.initials: EO
authors.lastname: Santos
authors.forename: Surza L G
authors.initials: SL
authors.lastname: Rocha
authors.forename: Jonas
authors.initials: J
authors.lastname: Perales
authors.forename: Gilberto B
authors.initials: GB
authors.lastname: Domont
authors.forename: Katia R S
authors.initials: KR
authors.lastname: Teixeira
authors.forename: Marcelo
authors.initials: M
authors.lastname: Bertalan
authors.forename: Paulo C G
authors.initials: PC
authors.lastname: Ferreira
authors.forename: Paulo M
authors.initials: PM
authors.lastname: Bisch
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D041821:Gluconacetobacter / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D031786:Saccharum / Q000254:growth & development* / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D020543:Proteome