Bone marrow stromal cells can be delivered to the site of traumatic brain injury via intrathecal transplantation in rabbits.
Recent studies suggest that bone marrow stromal cells (BMSCs) are promising grafts for treatment of traumatic brain injury (TBI). Neural precursor cells (NPCs) have been detected in the site of cervical cord injury following intrathecal injection by lumbar puncture. So, this study is designed to determine whether BMSCs (after intrathecal administration by lumbar puncture) could also migrate to the TBI site. The cells were cultured in vitro and transfected with adenovirus green fluorescent protein (Ad-GFP), and then transplanted intrathecally or intravenously into an autologous rabbit model of TBI. The labeled, grafted cells were identified in the injured cerebral tissue using fluorescence microscopy. Results showed that the intrathecal protocol was more efficient than the intravenous one. And motor dysfunction was improved after autologous transplantation of BMSCs. This study suggests another attractive minimally invasive option for treating TBI.
Liu
Wenpeng
W
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China. zhandhuimeng1688@sina.com
Jiang
Xiaodan
X
Fu
Xiafei
X
Cui
Shaojie
S
Du
Mouxuan
M
Cai
Yingqian
Y
Xu
Ruxiang
R
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D001930:Brain Injuries / Q000628:therapy*; D005434:Flow Cytometry; D006085:Graft Survival; D049452:Green Fluorescent Proteins / Q000235:genetics; D007278:Injections, Spinal; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology* / Q000637:transplantation*; D014162:Transfection
D049452:Green Fluorescent Proteins