title: Inhibition of myoblast differentiation by Sfrp1 and Sfrp2.
abstract: Secreted Frizzled-related proteins (Sfrps) are extracellular regulators of Wnt signalling and play important roles in developmental and oncogenic processes. They are known to be upregulated in regenerating muscle and in myoblast cultures but their function is unknown. Here, we show that the addition of recombinant Sfrp1 or Sfrp2 to C2C12 cell line cultures or to primary cultures of satellite cells results in the inhibition of myotube formation with no significant effect on the cell cycle or apoptosis. Even though at confluence, treated and untreated cultures are identical in appearance, analyses have shown that, for maximum effect, the cells have to be treated while they are proliferating. Furthermore, removal of Sfrp from the culture medium during differentiation restores normal myotube formation. We conclude that Sfrp1 and Sfrp2 act to prevent myoblasts from entering the terminal differentiation process.
authors.affiliation: INRA, UMR 866 Différenciation cellulaire et croissance, 2 place Viala, 34060 Montpellier, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Descamps
authors.forename: Hayat
authors.initials: H
authors.lastname: Arzouk
authors.forename: Francis
authors.initials: F
authors.lastname: Bacou
authors.forename: Henri
authors.initials: H
authors.lastname: Bernardi
authors.forename: Yann
authors.initials: Y
authors.lastname: Fedon
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Gay
authors.forename: Yves
authors.initials: Y
authors.lastname: Reyne
authors.forename: Bernadette
authors.initials: B
authors.lastname: Rossano
authors.forename: Jonathan
authors.initials: J
authors.lastname: Levin
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D000094742:Secreted Frizzled-Related Proteins
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C485539:Sfrp1 protein, mouse; D000094742:Secreted Frizzled-Related Proteins; C491628:Sfrp2 protein, mouse