[Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin].
OBJECTIVE
To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC).


METHODS
The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate: n-butanol: water (2 : 1 : 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm.


RESULTS
136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes.


CONCLUSIONS
The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.
Xu
You-Wei
YW
College of Pharmacy, Dalian Medical University, Dalian 116027, China.
Qi
Yan
Y
Han
Xu
X
Xu
Li-Na
LN
Xu
Qi-Wei
QW
Peng
Jin-Yong
JY
D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D030012:Gentiana / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic; D027343:Rhizome / Q000737:chemistry
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D012117:Resins, Synthetic; C012997:gentiopicroside