title: HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure.
abstract: An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm x 4.6 mm id, 5 mum) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at lambda = 386 nm while exciting at lambda = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.
authors.affiliation: Laboratory of Pharmaco-Toxicological Analysis, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Alma Mater Studiorum - University of Bologna, Bologna, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Mercolini
authors.forename: Maria
authors.initials: M
authors.lastname: Addolorata Saracino
authors.forename: Francesca
authors.initials: F
authors.lastname: Bugamelli
authors.forename: Anna
authors.initials: A
authors.lastname: Ferranti
authors.forename: Marco
authors.initials: M
authors.lastname: Malaguti
authors.forename: Silvana
authors.initials: S
authors.lastname: Hrelia
authors.forename: Maria Augusta
authors.initials: MA
authors.lastname: Raggi
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D007536:Isomerism; D008550:Melatonin / Q000032:analysis* / Q000737:chemistry*; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D059808:Polyphenols; D000077185:Resveratrol; D052616:Solid Phase Extraction / Q000379:methods*; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D008550:Melatonin; D000077185:Resveratrol