title: Hepatozoon ursi n. sp. (Apicomplexa: Hepatozoidae) in Japanese black bear (Ursus thibetanus japonicus).
abstract: Morphological and genetic features of a new Hepatozoon species, Hepatozoon ursi n. sp., in Japanese black bear (Ursus thibetanus japonicus) were studied. Schizogonic developmental stages were observed in the lungs of Japanese black bears. The schizonts were sub-spherical in shape and 45.7+/-4.6 x 42.7+/-4.5 microm in size. Each mature schizont contained approximately 80-130 merozoites and 0-5 residual bodies. The merozoites were 7.0+/-0.7 x 1.8+/-0.3 microm in size. Intraleukocytic gametocytes were slightly curved, cigar-like in shape and had a beak-like protrusion at one end. The size of the gametocytes was 10.9+/-0.3 x 3.3+/-0.2 microm. The analyses of the18S rRNA gene sequences supported the hypothesis that H. ursi n. sp. is different from other Hepatozoon species. Mature Hepatozoon oocysts were detected in two species of ticks (Haemaphysalis japonica and Haemaphysalis flava) collected on the bears infected with H. ursi n. sp. Two measured oocysts were 263.2 x 234.0 microm and 331.8 x 231.7 microm, respectively. The oocysts contained approximately 40 and 50 sporocysts, respectively. The sporocysts were sub-spherical in shape and 31.2+/-2.5 x 27.0+/-2.9 microm in size. Each sporocyst contained at least 8-16 sporozoites, with the sporozoites being 12.2+/-1.4 x 3.5+/-0.5 microm in size. H. ursi n. sp. is the first Hepatozoon species recorded from the family Ursidae.
authors.affiliation: United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Kubo
authors.forename: Shigehiko
authors.initials: S
authors.lastname: Uni
authors.forename: Takeshi
authors.initials: T
authors.lastname: Agatsuma
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nagataki
authors.forename: Roger J
authors.initials: RJ
authors.lastname: Panciera
authors.forename: Toshio
authors.initials: T
authors.lastname: Tsubota
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nakamura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sakai
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Masegi
authors.forename: Tokuma
authors.initials: T
authors.lastname: Yanai
mesh_terms: D000818:Animals; D003044:Coccidia / Q000145:classification* / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D003048:Coccidiosis / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D007564:Japan; D008168:Lung / Q000469:parasitology / Q000473:pathology; D052940:Merozoites / Q000648:ultrastructure; D008969:Molecular Sequence Data; D033661:Oocysts / Q000648:ultrastructure; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D052939:Schizonts / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013987:Ticks / Q000469:parasitology; D001503:Ursidae / Q000469:parasitology*
chemical_list: D012336:RNA, Ribosomal, 16S