[Identification of metastasis-associated proteins of ovarian cancer by proteomics].
OBJECTIVE
To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.


METHODS
The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.


RESULTS
Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.


CONCLUSIONS
The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.
Zhou
Le
L
The Key Laboratory of Biomedical Information Engineering of National Administration of Education, Institute for Cancer Research, Xi'an Jiaotong University, Xi'an, China.
Yang
Xiao-Feng
XF
Wang
Yan
Y
Zhang
Ying-Tao
YT
Geng
Yi-Ping
YP
Si
Lü-Sheng
LS
Wang
Yi-Li
YL
D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization