Characterization of tomato zonate spot virus, a new tospovirus in China.
An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. The complete nucleotide sequences of large (L), medium (M), and small (S) RNAs of this virus were found to contain 8919, 4945, and 3279 nts respectively. The L RNA encoded the RNA-dependent RNA polymerase (RdRp) (2885 aa, 332.7 kDa). The M RNA encoded a non-structural (NSm) protein (309 aa, 34.4 kDa) and a viral glycoprotein precursor (Gn/Gc) (1122 aa, 127.4 kDa). The S RNA encoded a non-structural protein (NSs) (459 aa, 51.9 kDa) and the nucleocapsid (N) protein (278 aa, 30.6 kDa). This N protein shared amino acid identities of 80.9% with those of calla lily chlorotic spot virus. Our results suggest that the virus studied here belongs to a new tospovirus species, for which the name tomato zonate spot virus is proposed.
Dong
Jia-Hong
JH
Yunnan Provincial Key Laboratory of Agri-Biotechnology, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650223, People's Republic of China.
Cheng
Xiao-Fei
XF
Yin
Yue-Yan
YY
Fang
Qi
Q
Ding
Ming
M
Li
Ting-Ting
TT
Zhang
Li-Zhen
LZ
Su
Xiao-Xia
XX
McBeath
Jenifer Huang
JH
Zhang
Zhong-Kai
ZK
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002212:Capsicum / Q000821:virology; D002681:China; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D009626:Terminology as Topic; D017865:Tospovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins