Extraction of DNA from soil using nanoparticles by magnetic bioseparation.
OBJECTIVE
To develop a simple, rapid and inexpensive soil DNA extraction protocol.


RESULTS
The protocol relies on the use of superparamagnetic silica-magnetite nanoparticles for the isolation and purification of DNA from soil samples. DNA suitable for use in molecular biology applications was obtained from a number of soil samples.


CONCLUSIONS
The DNA extracted using the tested method successfully permitted the PCR amplification of a fragment of the bacterial 16S rDNA gene. The extracted DNA could also be restriction endonuclease digested.


CONCLUSIONS
The protocol reported here is simple and permits rapid isolation of PCR-ready soil DNA. The method requires only small quantities of soil sample, is scalable and suitable for automation.
Sebastianelli
A
A
Nanobiotechnology Research Group, Department of Biosciences, University of Kent, Canterbury, UK.
Sen
T
T
Bruce
I J
IJ
D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D008280:Magnetics; D053758:Nanoparticles; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D004247:DNA