title: Effects of mycotoxins on mitogen-stimulated proliferation of bovine peripheral blood mononuclear cells.
abstract: The effects of mycotoxins on mitogen-stimulated proliferation of bovine peripheral blood mononuclear cells (PBMCs) were investigated. Aflatoxin B(1) (AFB(1)), deoxynivalenol (DON) and zearalenone (ZEN) were added to cultures of PBMCs, and the proliferation responses were measured using MTT bioassays. Suppression of the proliferation of calf PBMCs by AFB(1) and DON was significantly stronger than that of cow PBMCs, whereas there were no differences in suppressive effects on PBMCs from Holstein and Japanese Black calves and cows. The suppressive effect was greatest in the order of DON, AFB(1) and ZEN, and the effects of DON and AFB(1) seemed to be dose-dependent. The results suggest that some mycotoxins directly suppress proliferation of bovine PBMCs.
authors.affiliation: Yamagata Prefecutural Federation of Agricultural Mutual Aid Association, Yamagata-shi, Yamagata, Japan. wada_k@yynosai.or.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Wada
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Hashiba
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kohiruimaki
authors.forename: Machiko
authors.initials: M
authors.lastname: Masui
authors.forename: Seiichi
authors.initials: S
authors.lastname: Kawamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Endo
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ogata
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D009183:Mycotoxins / Q000494:pharmacology*
chemical_list: D008934:Mitogens; D009183:Mycotoxins