title: Simple and rapid field tests for brucellosis in livestock.
abstract: Four simple and rapid field tests for the serodiagnosis of brucellosis in cattle, goat, sheep and swine were developed. The performance of the assays was investigated using serum samples collected in Portugal from animals originating from herds with a defined sanitary status with respect to the presence of brucellosis. The sensitivity calculated for the bovine, caprine, ovine and swine Brucella lateral flow assays based on results obtained for samples collected from animals with culture confirmed brucellosis was 90%, 100%, 90% and 73%, respectively. None of the samples from animals from herds free of brucellosis reacted in the flow assays indicating a high specificity. However, as expected, some degree of reactivity was observed when testing selected serum samples that reacted non-specific in reference tests for brucellosis.
authors.affiliation: KIT Biomedical Research, Royal Tropical Institute/Koninklijk Instituut voor de Tropen (KIT), Meibergdreef 39, Amsterdam, The Netherlands.
authors.forename: Theresia
authors.initials: T
authors.lastname: Abdoel
authors.forename: Isabel Travassos
authors.initials: IT
authors.lastname: Dias
authors.forename: Regina
authors.initials: R
authors.lastname: Cardoso
authors.forename: Henk L
authors.initials: HL
authors.lastname: Smits
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003168:Complement Fixation Tests / Q000662:veterinary; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D012395:Rose Bengal; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012395:Rose Bengal