[Use W303-1A/hER-ERE-Lac Z to determine estrogenic compounds in traditional Chinese materia medica].
OBJECTIVE
To study the content of phytoestrogen in dissimilarity herbs.


METHODS
The activity of phytoestrogen in heat-clearing drugs, drugs for relieving exterior syndrome, diuretic, anastaltics, tonics and astringents were detected based on the recombinant yeast cell (W303-1A/hER-ERE-Lac Z). The estrogenic activity in traditional Chinese materia medica were assayed quantitatively by determining the expression of beta-galactosidase.


RESULTS
The phytoestrogen concentration (6.35 x 10(-3) nmol x g(-1) E2 equivalent) in heat-clearing drugs was the highest while that in anastaltic and tonic drugs was the lowest, which was less than the detected limit.


CONCLUSIONS
Compared with the other traditional Chinese materia medica, the content of phytoestrogen, which can bind to estrogen receptor, in giant knotweed rhizome, forsythia suspense, ash bark, baical skullcap root and ophiopogonis tuber were higher.
Zhu
Ping-Ting
PT
Medical College of Yangzhou University, Jiangsu Yangzhou 225001, China.
Bo
Ping
P
Chen
Xiu-Yun
XY
Wu
Yu-Juan
YJ
Chen
Chun-Bo
CB
Li
Xiang-Ming
XM
D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D048789:Phytoestrogens / Q000032:analysis* / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D011960:Receptors, Estrogen / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects / Q000235:genetics; D001616:beta-Galactosidase / Q000032:analysis*
D004365:Drugs, Chinese Herbal; D048789:Phytoestrogens; D011960:Receptors, Estrogen; D001616:beta-Galactosidase