Determination of pentachlorophenol and tribromophenol in sawdust by ultrasound-assisted extraction and MEKC.
An ultrasonic bar-assisted extraction and CE separation procedure for the determination of pentachlorophenol (PCP) and 2,4,6-tribromophenol (TBP) residues in sawdust was developed and applied. For this purpose, micellar electrokinetic capillary chromatography (MEKC) was used and compared with a GC/MS methodology. This methodology allowed the quantification of PCP and TBP in a concentration range of 2.5-12.0 mg/kg for TBP and 2.8-12.0 mg/kg for PCP. Different sample treatment processes were evaluated in order to extract these compounds from sawdust. Better results were obtained when the residues were extracted with ultrasound-assisted hexane, filtered, evaporated, dissolved in Na(2)CO(3), and injected into the CE equipment. The optimal option for GC/MS was extraction with Na(2)CO(3 )followed by a derivation using acetic anhydride and liquid-liquid extraction with hexane. This method allowed the quantification of TBP and PCP in sawdust in a concentration range of 0.19-12.00 mg/kg and 0.14-12.00 mg/kg, respectively. The CE method was compared with the GC/MS as reference method. The results were shown to be statistically similar by both methods for PCP as well as for TBP.
Mardones
Claudia
C
Departamento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile. cmardone@udec.cl
von Baer
Dietrich
D
Hidalgo
Ariela
A
Contreras
Alejandra
A
Sepúlveda
Carlos
C
D002138:Calibration; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004391:Dust / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry*; D014465:Ultrasonics; D014934:Wood / Q000737:chemistry*
D004391:Dust; D010636:Phenols