title: Age-related alterations in Ca2+ signals and mitochondrial membrane potential in exocrine cells are prevented by melatonin.
abstract: Information regarding age-induced Ca(2+) signal alterations in nonexcitable cells is limited. In addition, little evidence exists on the ability of melatonin to palliate the effects of aging on Ca(2+) signals and mitochondrial potential, a parameter involved in both Ca(2+) signaling and aging. We studied the ability of melatonin to prevent the effects of aging on intracellular Ca(2+) homeostasis and mitochondrial potential in exocrine cells. Pancreatic acinar cells were obtained from adult (3 months old) and aged (22-24 months old) mice by collagenase dispersion. Ca(2+) signals, in situ mitochondrial potential and in vitro amylase secretion were determined. Secretion in response to increasing levels of the secretagogues, acetylcholine and cholecystokinin (CCK), were impaired in aged pancreatic acini. This decrease was accompanied by an inhibition in the amplitude of the peak response to maximal concentrations of the agonists, and by a decrease in the pattern of Ca(2+) oscillations induced by postprandial levels of CCK. Both the size of the calcium pools, assessed by low levels of ionomycin, and capacitative calcium entry, induced by depletion of the stores with thapsigargin, were diminished in aged cells. These changes in Ca(2+) homeostasis were associated with depolarization of intracellular mitochondria. Oral administration of melatonin for 3 months to aged mice restored the secretory response, the amplitude and frequency of Ca(2+) responses, the size of intracellular calcium pools, the capacitative calcium entry, and the mitochondrial potential. In conclusion, melatonin restores secretory function, Ca(2+) signals and mitochondrial potential of aged exocrine cells.
authors.affiliation: Department of Physiology, Faculty of Veterinary Science, Nursing School and RETICEF, University of Extremadura, Caceres, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Camello-Almaraz
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Gomez-Pinilla
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Pozo
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Camello
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000681:Amylases / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects*; D002478:Cells, Cultured; D002766:Cholecystokinin / Q000494:pharmacology; D005088:Exocrine Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051379:Mice
chemical_list: D000975:Antioxidants; D002766:Cholecystokinin; D000681:Amylases; D008550:Melatonin; D000109:Acetylcholine