title: Exogenous leptin promotes the recovery of regressed ovary in fasted ducks.
abstract: The present study was undertaken to examine the effect of administered recombinant mouse leptin on the recovery of regressed ovary in fasted ducks. Twenty-eight ducks were divided into five groups: fed ad libitum (control; n=5), fasted control (FC; n=5), fasted+low dose of leptin (F+L; n=5), fasted+medium dose of leptin (F+M; n=5) and fasted+high dose of leptin (F+H; n=3). All four fasted groups were fasted for 2 days and then ad libitum and the ducks were treated with leptin at doses of 0 (control and FC), 50 (F+L), 250 (F+M) and 1000 (F+H) microg/kg body weight/day on day 3-5. Results showed that a moderate dose of leptin (250 microg/kg body weight/day) injected during the re-feeding period: (i) promoted the recovery of the regressed ovary as evidenced by an increase in ovary weight and recovery of yellow hierarchical follicles; (ii) elevated the plasma 17beta-estradiol (E(2)) level; (iii) increased the mRNA levels of ovary follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR) and estrogen receptor-beta (ER-beta). Furthermore, the results also showed that a high dose of leptin (1000 microg/kg body weight/day) may have a negative effect on the recovery of the regressed ovary. In conclusion, this study indicates that, in ducks, leptin may be involved in the recovery of the regressed ovary caused by 2 days of fasting. This effect may be related to increased plasma E(2) levels and stimulation of the mRNA levels of ovarian FSHR, LHR and especially ER-beta.
authors.affiliation: Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Song
authors.forename: Chonggang
authors.initials: C
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.forename: Liangju
authors.initials: L
authors.lastname: Lv
authors.forename: Yixin
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Zuo
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D004372:Ducks / Q000235:genetics / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D047629:Estrogen Receptor beta / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005508:Food Deprivation / Q000502:physiology*; D020738:Leptin / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects* / Q000502:physiology; D010058:Oviposition / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011962:Receptors, FSH / Q000096:biosynthesis / Q000235:genetics; D011974:Receptors, LH / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D047629:Estrogen Receptor beta; D020738:Leptin; D012333:RNA, Messenger; D011962:Receptors, FSH; D011974:Receptors, LH; D011994:Recombinant Proteins; D004958:Estradiol