In situ mineralization of hydroxyapatite on electrospun chitosan-based nanofibrous scaffolds.
A biocomposite of hydroxyapatite (HAp) with electrospun nanofibrous scaffolds was prepared by using chitosan/polyvinyl alcohol (CS/PVA) and N-carboxyethyl chitosan/PVA (CECS/PVA) electrospun membranes as organic matrix, and HAp was formed in supersaturated CaCl2 and KH2PO4 solution. The influences of carboxylic acid groups in CECS/PVA fibrous scaffold and polyanionic additive poly(acrylic acid) (PAA) in the incubation solution on the crystal distribution of the HAp were investigated. Field-emission scanning electron microscopy (FE-SEM), energy-dispersive spectroscopy (EDS), wide-angle X-ray diffraction (WAXD), and Fourier transform infrared (FTIR) were used to characterize the morphology and structure of the deposited mineral phase on the scaffolds. It was found that addition of PAA to the mineral solution and use of matrix with carboxylic acid groups promoted mineral growth and distribution of HAp. MTT testing and SEM imaging from mouse fibroblast (L929) cell culture revealed the attachment and growth of mouse fibroblast on the surface of biocomposite scaffold, and that the cell morphology and viability were satisfactory for the composite to be used in bioapplications.
Yang
Dongzhi
D
State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Beijing City on Preparation and Processing of Novel Polymer Materials, College of Material Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Jin
Yu
Y
Zhou
Yingshan
Y
Ma
Guiping
G
Chen
Xiangmei
X
Lu
Fengmin
F
Nie
Jun
J
D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D002122:Calcium Chloride / Q000737:chemistry; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D005347:Fibroblasts; D051379:Mice; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010710:Phosphates / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013057:Spectrum Analysis; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
D000180:Acrylic Resins; D010710:Phosphates; D011145:Polyvinyls; D017680:Potassium Compounds; C511088:chitosan-g-poly(vinyl alcohol) copolymer; C006903:carbopol 940; D048271:Chitosan; D017886:Durapatite; C013216:potassium phosphate; D002122:Calcium Chloride