Expression and distributional patterns of the inhibitor of apoptosis protein family and caspase 3 in nasal polyps.
OBJECTIVE
To investigate the expression and distributional patterns of the inhibitor of apoptosis protein (IAP) family and caspase 3 in nasal polyps and normal nasal mucosa and to evaluate the possible effects of the IAP family and caspase 3 on the development of nasal polyps.


METHODS
Prospective study.


METHODS
Tertiary academic institution.


METHODS
Normal inferior turbinate mucosa was obtained from 20 patients undergoing surgery for augmentation rhinoplasty. Nasal polyps were obtained from 20 patients undergoing endoscopic sinus surgery for chronic polypoid sinusitis.


METHODS
Reverse transcriptase-polymerase chain reaction, immunohistochemical analysis, and Western blot analysis were performed.


METHODS
The expression and distribution of cIAP1, cIAP2, XIAP, survivin, and caspase 3 were evaluated in normal turbinate mucosa and nasal polyps.


RESULTS
cIAP1, cIAP2, and XIAP were expressed in normal human nasal mucosa, where they were detected in submucosal glands, epithelial cells, vascular endothelial cells, and inflammatory cells. However, cIAP1 was not expressed in nasal polyps, whereas cIAP2 and XIAP were expressed in submucosal glands, epithelial cells, vascular endothelial cells, and inflammatory cells. Caspase 3 was localized to a portion of the epithelial cells in normal nasal mucosa and nasal polyps. Survivin was not expressed in any samples. Furthermore, cIAP2, XIAP, and caspase 3 did not show a significant difference in their expression levels between normal nasal mucosa and nasal polyps.


CONCLUSIONS
The present results indicate that cIAP1, cIAP2, XIAP, and caspase 3 may regulate the homeostasis of normal nasal mucosa, whereas cIAP2, XIAP, and caspase 3 may take part in the pathogenesis of nasal polyps.
Cho
Seok Hyun
SH
Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Hanyang University, Seoul, South Korea.
Lee
Sang Hag
SH
Kim
Kyung Rae
KR
Lee
Heung Man
HM
Lee
Seung Hoon
SH
Kim
Tae Hoon
TH
D000328:Adult; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D008297:Male; D009298:Nasal Polyps / Q000201:enzymology / Q000378:metabolism*; D011446:Prospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric
D051034:Inhibitor of Apoptosis Proteins; D053148:Caspase 3