Characteristics of superficially-porous silica particles for fast HPLC: some performance comparisons with sub-2-microm particles.
Columns of 2.7-microm fused-core (superficially porous) Type B silica particles allow very fast separations of small molecules at pressures available in most high-performance liquid chromatography instruments. These highly-purified particles with 1.7-microm solid silica cores and 0.5-microm-thick shells of 9 nm pores exhibit efficiencies that rival those of totally porous sub-2-microm particles but at one-half to one-third of the column back pressure. This presentation describes other operating features of fused-core particle columns, including sample loading characteristics and packed bed stability. The superior mass transfer (kinetic) properties of the fused-core particles result in much-improved separation efficiency at higher mobile phase velocities, especially for > 600 molecular weight solutes.
Destefano
J J
JJ
Advanced Materials Technology, Inc., 3521 Silverside Rd., Suite 1-K, Quillen Bldg., Wilmington, DE 19810, USA. joedestefano@advanced-materials-tech.com
Langlois
T J
TJ
Kirkland
J J
JJ