Investigation of the interaction between ssDNA and 2-aminophenoxazine-3-one and development of an electrochemical DNA biosensor.
An electrochemical method was used to probe the interaction between 2-aminophenoxazine-3-one (AP) and the short DNA sequence related to the hepatitis B virus (HBV), and an electrochemical DNA biosensor was developed. The voltammetric signals of AP have been investigated at bare glassy carbon electrode (bare GCE), hybrid double-stranded DNA-modified GCE (dsDNA/GCE), and single-stranded DNA-modified GCE (ssDNA/GCE) by means of differential pulse voltammetry (DPV), and the peak currents increased with respect to the order of electrodes. The extent of hybridization was evaluated on the basis of the difference between signals of AP with a probe before and after hybridization with the complementary sequence. Control experiments with noncomplementary were performed to test the selectivity of the biosensor. With this approach, a sequence of the HBV could be quantified over the range from 3.53 x 10(-7) to 1.08 x 10(-6) M, with a linear correlation of r = 0.9963 and a detection limit of 1.00 x 10(-7) M.
Li
Xue-Mei
XM
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.
Ju
Heng-Qiang
HQ
Zhang
Shu-Sheng
SS
D001483:Base Sequence; D015374:Biosensing Techniques; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004563:Electrochemistry; D009693:Nucleic Acid Hybridization; D010078:Oxazines / Q000737:chemistry*
D004277:DNA, Single-Stranded; D010078:Oxazines; C005512:3-aminophenoxazone