title: Characterization of a titanium(IV)-porphyrin complex as a highly sensitive and selective reagent for the determination of hydrogen peroxide: a computational chemistry approach and a critical review.
abstract: The Ti-TPyP reagent, i.e. an acidic aqueous solution of the oxo[5,10,15,20-tetra(4-pyridyl)porphyrinato] titanium(IV) complex, TiO(tpyp), was developed as a highly sensitive and selective spectrophotometric reagent for determination of traces of hydrogen peroxide. Using this reagent, determination of hydrogen peroxide was performed by flow-injection analysis with a detection limit of 0.5 pmol per test. The method was actually applied to determination of several constituents of foods, human blood, and urine mediated by appropriate oxidase enzymes. The reaction specificity of the TiO(tpyp) complex for hydrogen peroxide was clarified from the viewpoint of the reaction mechanisms and molecular orbitals based on ab initio calculations. The results provided a well-grounded argument for determination of hydrogen peroxide using the Ti-TPyP reagent experimentally. This review deals with characterization of the high sensitivity and reaction specificity of the Ti-TPyP reagent for determination of hydrogen peroxide, to prove its reliability in analytical applications.
authors.affiliation: Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo, 192-0392, Japan. takamura@ps.toyaku.ac.jp
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Takamura
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D001786:Blood Glucose / Q000032:analysis; D003198:Computer Simulation; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D005690:Galactose / Q000097:blood; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D008956:Models, Chemical; D009942:Organometallic Compounds / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014025:Titanium / Q000737:chemistry*; D014527:Uric Acid / Q000097:blood
chemical_list: D001786:Blood Glucose; D009942:Organometallic Compounds; D011166:Porphyrins; D014527:Uric Acid; D006861:Hydrogen Peroxide; D014025:Titanium; D005690:Galactose