title: A dual-tag microarray platform for high-performance nucleic acid and protein analyses.
abstract: DNA microarrays serve to monitor a wide range of molecular events, but emerging applications like measurements of weakly expressed genes or of proteins and their interaction patterns will require enhanced performance to improve specificity of detection and dynamic range. To further extend the utility of DNA microarray-based approaches we present a high-performance tag microarray procedure that enables probe-based analysis of as little as 100 target cDNA molecules, and with a linear dynamic range close to 10(5). Furthermore, the protocol radically decreases the risk of cross-hybridization on microarrays compared to current approaches, and it also allows for quantification by single-molecule analysis and real-time on-chip monitoring of rolling-circle amplification. We provide proof of concept for microarray-based measurement of both mRNA molecules and of proteins, converted to tag DNA sequences by padlock and proximity probe ligation, respectively.
authors.affiliation: Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Ericsson
authors.forename: Jonas
authors.initials: J
authors.lastname: Jarvius
authors.forename: Edith
authors.initials: E
authors.lastname: Schallmeiner
authors.forename: Mathias
authors.initials: M
authors.lastname: Howell
authors.forename: Rachel Yuan
authors.initials: RY
authors.lastname: Nong
authors.forename: Hendrik
authors.initials: H
authors.lastname: Reuter
authors.forename: Meinhard
authors.initials: M
authors.lastname: Hahn
authors.forename: Johan
authors.initials: J
authors.lastname: Stenberg
authors.forename: Mats
authors.initials: M
authors.lastname: Nilsson
authors.forename: Ulf
authors.initials: U
authors.lastname: Landegren
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D020869:Gene Expression Profiling / Q000379:methods*; D015335:Molecular Probes; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010982:Platelet-Derived Growth Factor / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D000199:Actins; D015335:Molecular Probes; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A