title: Expression, purification, crystallization and preliminary X-ray characterization of two crystal forms of stationary-phase survival E protein from Campylobacter jejuni.
abstract: Survival E (SurE) protein from Campylobacter jejuni, a Gram-negative mesophile, has been overexpressed in Escherichia coli as a soluble protein, successfully purified and crystallized in two distinct crystal forms. The first form belongs to space group P2(1)2(1)2(1), with a tetramer in the asymmetric unit and unit-cell parameters a = 80.5, b = 119.0, c = 135.3 A. The second form belongs to space group C2, with unit-cell parameters a = 121.4, b = 47.1, c = 97.8 A, and contains a dimer in the asymmetric unit. Diffraction data have been collected from these crystal forms to 2.5 and 2.95 A resolution, respectively.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Avenida da República, Apartado 127, 2781-901 Oeiras, Portugal.
authors.forename: A M D
authors.initials: AM
authors.lastname: Gonçalves
authors.forename: A T
authors.initials: AT
authors.lastname: Rêgo
authors.forename: M
authors.initials: M
authors.lastname: Thomaz
authors.forename: F J
authors.initials: FJ
authors.lastname: Enguita
authors.forename: M A
authors.initials: MA
authors.lastname: Carrondo
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D003460:Crystallization; D015870:Gene Expression; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins