A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents.
The goal of many current studies of neovascularization is to define the phenotype of vascular cell populations of different origins and to determine how such cells promote assembly of vascular channel. Here, we describe a protocol to immunophenotype vascular cells by high-resolution imaging and by fluorescence-activated flow cytometry in an in vivo rodent model of pulmonary microvascular remodeling. Analysis of cells by this combined approach will characterize their phenotype, quantify their number and identify their role in the assembly of vascular channels.
Jones
Rosemary C
RC
Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, Massachusetts 02114, USA. rcjones@partners.org
Capen
Diane E
DE
Cohen
Kenneth S
KS
Munn
Lance L
LL
Jain
Rakesh K
RK
Duda
Dan G
DG
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000378:metabolism; D042783:Endothelial Cells / Q000145:classification* / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods*; D008168:Lung / Q000098:blood supply* / Q000166:cytology; D051379:Mice; D046529:Microscopy, Electron, Transmission / Q000379:methods; D018919:Neovascularization, Physiologic / Q000502:physiology*; D010641:Phenotype; D010781:Photography / Q000379:methods*; D051381:Rats
D000906:Antibodies