A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus.
The 1.1 MDa cell-wall-associated adhesion protein of staphylococci, Ebh, consists of several distinct regions, including a large central region with 52 imperfect repeats of 126 amino acid residues. We investigated the structure of this giant molecule by X-ray crystallography, circular dichroism (CD) spectrometry, and small-angle X-ray scattering (SAXS). The crystal structure of two repeats showed that each repeat consists of two distinct three-helix bundles, and two such repeats are connected along the long axis, resulting in a rod-like structure that is 120 A in length. CD and SAXS analyses of the samples with longer repeats suggested that each repeat has an identical structure, and that such repeats are connected tandemly to form a rod-like structure in solution, the length of which increased proportionately with the number of repeating units. On the basis of these results, it was proposed that Ebh is a 320 nm rod-like molecule with some plasticity at module junctions.
Tanaka
Yoshikazu
Y
Faculty of Advanced Life Sciences, Hokkaido University, Sapporo 060-0810, Japan.
Sakamoto
Sou
S
Kuroda
Makoto
M
Goda
Shuichiro
S
Gao
Yong-Gui
YG
Tsumoto
Kouhei
K
Hiragi
Yuzuru
Y
Yao
Min
M
Watanabe
Nobuhisa
N
Ohta
Toshiko
T
Tanaka
Isao
I
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002942:Circular Dichroism; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D020449:Repetitive Sequences, Amino Acid / Q000502:physiology; D053838:Scattering, Small Angle; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; C469917:Ebh protein, Staphylococcus aureus