The fast skeletal muscle myosin light chain is differentially expressed in smooth muscle cells of OVA-challenged mouse trachea.
In a search for new molecular pathways associated with asthma, we performed an mRNA differential display analysis using total RNA extracted from the tracheal tissues of ovalbumin (OVA)-challenged mice and sham controls. cDNAs corresponding to mRNAs for which expression levels were altered by OVA-challenge were isolate and sequenced. Twenty-eight genes differentially expressed in sham and OVA challenged mice were identified. A GenBank BLAST homology search revealed that they were related to cytoskeleton remodeling, transcription, protein synthesis and modification, energy production, and cell growth and differentiation. Two were selected for further characterization. Up-regulation of both the perinatal skeletal myosin heavy chain (skMHC) and fast skeletal muscle myosin light chain (skMLC) genes was confirmed by RT-PCR of trachea tissue from OVA challenged mice. Overexpression of skMLC protein was observed in the smooth muscle layers of OVA-challenged mice by immunohistochemistry, and the surface areas stained with skMLC antibody increased in the OVA-challenged mice. The overexpression of skMLC in murine asthma may be associated with the changes of bronchial smooth muscle.
Kim
Ho-Young
HY
Genome Research Center for Allergy and Respiratory Diseases, Soonchunhyang University Hospital, Bucheon 420-767, Korea.
Rhim
Taiyoun
T
Ahn
Mi-Hyun
MH
Yoon
Pyoung-Oh
PO
Kim
Soo-Ho
SH
Lee
Sang-Han
SH
Park
Choon-Sik
CS
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D018994:Myosin Light Chains / Q000235:genetics / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology* / Q000494:pharmacology; D015203:Reproducibility of Results; D014132:Trachea / Q000033:anatomy & histology* / Q000187:drug effects / Q000276:immunology
D018994:Myosin Light Chains; D010047:Ovalbumin