K6PC-5, a novel sphingosine kinase activator, improves long-term ultraviolet light-exposed aged murine skin.
Sphingosine-1-phosphate (S1P), which is formed by phosphorylation of sphingosine through a process catalysed by sphingosine kinase (SK), is a multifunctional mediator of a variety of cellular responses including proliferation, differentiation, motility, and survival. K6PC-5, which was recently synthesized as a novel SK activator, is expected to increase S1P levels. Indeed studies have already demonstrated that K6PC-5 exhibits anti-aging effects on intrinsic aged murine skin by increasing fibroblasts, collagen synthesis, dermal thickness, and epidermal differentiation. However, photoaging and intrinsic aging have highly different clinical and histopathological properties. In this study, we developed a photoaged murine model by exposing mice that were 56 weeks old to ultraviolet (UV)B and UVA radiation for 8 weeks. We then investigated whether K6PC-5, as an SK activator, had anti-aging effects on photoaged murine skin in addition to its effects on intrinsic aged murine skin and determined the mechanism. K6PC-5 increased dermal collagen density in photoaged skin through increases in fibroblasts and collagen production. Photoaged murine skin treated with K6PC-5 showed an increase in stratum corneum (SC) integrity with increased corneodesmosome density and an improvement in barrier recovery rate. Matrix metalloproteinase 13 remained unchanged. These results indicate that topical application of K6PC-5 improves photoaged skin by improving skin barrier and increasing fibroblast count and function. In conclusion, K6PC-5, as an S1P activator, improves long-term UV-exposed aged skin as well as intrinsic aged skin.
Park
Hwa-young
HY
Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju, Korea.
Youm
Jong-Kyung
JK
Kwon
Mi Jung
MJ
Park
Byeong Deog
BD
Lee
Seung Hun
SH
Choi
Eung Ho
EH
D000577:Amides / Q000494:pharmacology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects; D000078404:Epidermal Cells; D004817:Epidermis / Q000201:enzymology* / Q000528:radiation effects; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects; D008246:Lysophospholipids / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D015595:Skin Aging / Q000187:drug effects*; D013110:Sphingosine / Q000031:analogs & derivatives / Q000378:metabolism; D014466:Ultraviolet Rays; D000099157:Sphingosine Kinase
D000577:Amides; D015415:Biomarkers; D003094:Collagen; D008246:Lysophospholipids; D017853:Phosphotransferases (Alcohol Group Acceptor); D013110:Sphingosine; D000099157:Sphingosine Kinase; C531172:N-(1,3-dihydroxyisopropyl)-2-hexyl-3-oxodecanamide; C060506:sphingosine 1-phosphate