Modulation of protein stability by O-glycosylation in a designed Gc-MAF analog.
The post-translational modification of proteins by the covalent attachment of carbohydrates to specific side chains, or glycosylation, is emerging as a crucial process in modulating the function of proteins. In particular, the dynamic processing of the oligosaccharide can correlate with a change in function. For example, a potent macrophage-activating factor, Gc-MAF, is obtained from serum vitamin D binding protein (VDBP) by stepwise processing of the oligosaccharide attached to Thr 420 to the core alpha-GalNAc moiety. In previous work we designed a miniprotein analog of Gc-MAF, MM1, by grafting the glycosylated loop of Gc-MAF on a stable scaffold. GalNAc-MM1 showed native-like activity on macrophages (Bogani 2006, J. Am. Chem. Soc. 128 7142-43). Here, we present data on the thermodynamic stability and conformational dynamics of the mono- and diglycosylated forms. We observed an unusual trend: each glycosylation event destabilized the protein by about 1 kcal/mol. This effect is matched by an increase in the mobility of the glycosylated forms, as evaluated by molecular dynamics simulations. An analysis of the solvent-accessible surface area shows that glycosylation causes the three-helix bundle to adopt conformations in which the hydrophobic residues are more solvent exposed. The number of hydrophobic contacts is also affected. These two factors, which are ultimately explained with a change in occupancy for conformers of specific side chains, may contribute to the observed destabilization.
Spiriti
Justin
J
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, United States.
Bogani
Federica
F
van der Vaart
Arjan
A
Ghirlanda
Giovanna
G
D003198:Computer Simulation; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D016215:Macrophage-Activating Factors / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D013816:Thermodynamics
D006023:Glycoproteins; D016215:Macrophage-Activating Factors