Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue.
We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy.
Kim
Hwan Myung
HM
Department of Chemistry, National Creative Research Initiative Center for Cell Dynamics, Department of Physics, Biomicrosystems Technology Program, Korea University, 1-Anamdong, Seoul, 136-701, Korea.
Jeong
Byeong Ha
BH
Hyon
Ju-Yong
JY
An
Myoung Jin
MJ
Seo
Mun Sik
MS
Hong
Jin Hee
JH
Lee
Kyoung J
KJ
Kim
Chul Hoon
CH
Joo
Taiha
T
Hong
Seok-Cheol
SC
Cho
Bong Rae
BR
D000818:Animals; D002478:Cells, Cultured; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry*; D009924:Organ Culture Techniques; D017785:Photons; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats
C528316:6-((E)-3-oxo-1-dodecenyl)-2-(N-methyl-N-(carboxymethyl)amino)naphthalene; C528315:6-dodecanoyl-2-(N-methyl-N-(carboxymethyl)amino)naphthalene; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D009281:Naphthalenes