title: Effects of four carbamate compounds on antioxidant parameters.
abstract: The effect of four carbamates, aldicarb and its metabolites (aldicarb sulfone and aldicarb sulfoxide) and propoxur on glutathione content and the activity of the enzymes involved in the sulfur-redox cycle in the mammalian cellular model CHO-K1 cells after 24-h exposure were determined. Carbamate exposure resulted in a depletion of intracellular reduced glutathione (GSH) content, no change was observed in oxidized glutathione (GSSG) and a decrease in GSH/GSSG ratio was detected. After carbamates exposition a GSH/GSSG decreases in ranged from 12.44% to 21.35% of control was observed. Depletion of GSH levels was accompanied by the induction of glutathione reductase (GR) after 24h exposure with each of the four carbamates to CHO-K1 cells. After aldicarb sulfone, aldicarb sulfoxide, and propoxur exposure, glutathione peroxidase (GPx) activity increased in CHO-K1 cells by 198%, 32%, and 228% of control, respectively. After aldicarb sulfone and propoxur exposure, glutathione transferase (GST) activities increased by 49% and 230% of control, respectively. Due to the role played by GSH in preventing cytotoxicity via free-radical scavenging, results obtained suggest that high concentrations of aldicarb sulfone and propoxur closely resembling oxidative stress in CHO-K1 cells.
authors.affiliation: Sezione di Chimica Analitica e Ambientale, Dip. Scienze Chimiche, Universita degli Studi di Trieste, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Maran
authors.forename: Mónica
authors.initials: M
authors.lastname: Fernández
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Barbieri
authors.forename: Guillermina
authors.initials: G
authors.lastname: Font
authors.forename: María José
authors.initials: MJ
authors.lastname: Ruiz
mesh_terms: D000448:Aldicarb / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000502:physiology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004790:Enzyme Induction; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D001074:Propoxur / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D007306:Insecticides; C030579:aldicarb sulfoxide; D000448:Aldicarb; D001074:Propoxur; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; C030580:aldicarb sulfone; D019803:Glutathione Disulfide