title: Intercalibration exercise using a stickleback endocrine disrupter screening assay.
abstract: The Organisation for Economic Cooperation and Development (OECD) is currently validating a short-term fish screening protocol for endocrine disrupters (estrogens, androgens, and their antagonists and aromatase inhibitors), using three core species: fathead minnow, Japanese medaka, and zebrafish. The main endpoints proposed for the first phase of validation of the screen are vitellogenin (VTG) concentration, gross morphology (secondary sexual characteristics and gonado-somatic index), and gonadal histopathology. A similar protocol is concurrently being developed in the United Kingdom using the three-spined stickleback, with identical endpoints to those for the core species and, in addition, a unique androgen-specific endpoint in the form of spiggin (glue protein) induction. To assess the suitability of this species for inclusion in the OECD protocol alongside the core species, an intercalibration was conducted using 17beta-estradiol (a natural estrogen) and trenbolone (a synthetic androgen), thus mimicking a previous intercalibration with the core species. All three participating laboratories detected statistically significant increases in VTG in males after 14 d exposure to nominal concentrations of 100 ng/L 17beta-estradiol and statistically significant increases in spiggin in females after 14 d exposure to nominal concentrations of 5,000 ng/L trenbolone. The stickleback screen is reliable, possessing both relevant and reproducible endpoints for the detection of potent estrogens and androgens. Further work is underway to assess the relevance and suitability of the screen for weakly acting estrogens, anti-androgens, and aromatase inhibitors.
authors.affiliation: Centre for Environment, Fisheries, and Aquaculture Science, Burnham Laboratory, Burnham-on-Crouch, Essex, UK. yvonne.allen@cefas.co.uk
authors.forename: Yvonne T
authors.initials: YT
authors.lastname: Allen
authors.forename: Ioanna
authors.initials: I
authors.lastname: Katsiadaki
authors.forename: Tom G
authors.initials: TG
authors.lastname: Pottinger
authors.forename: Cecile
authors.initials: C
authors.lastname: Jolly
authors.forename: Peter
authors.initials: P
authors.lastname: Matthiessen
authors.forename: Ian
authors.initials: I
authors.lastname: Mayer
authors.forename: Andy
authors.initials: A
authors.lastname: Smith
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Scott
authors.forename: Paul
authors.initials: P
authors.lastname: Eccles
authors.forename: Matthew B
authors.initials: MB
authors.lastname: Sanders
authors.forename: Kim G T
authors.initials: KG
authors.lastname: Pulman
authors.forename: Stephen
authors.initials: S
authors.lastname: Feist
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004784:Environmental Monitoring / Q000379:methods; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D029941:Fish Proteins / Q000378:metabolism; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000502:physiology; D015203:Reproducibility of Results; D023701:Smegmamorpha / Q000502:physiology*; D013737:Testis / Q000187:drug effects / Q000502:physiology; D014204:Trenbolone Acetate / Q000494:pharmacology*; D014819:Vitellogenins / Q000378:metabolism
chemical_list: D052244:Endocrine Disruptors; D029941:Fish Proteins; D014819:Vitellogenins; C429543:spiggin; D004958:Estradiol; D014204:Trenbolone Acetate