Gold-nanorod-based sensing of sequence specific HIV-1 virus DNA by using hyper-Rayleigh scattering spectroscopy.
Infectious diseases caused by the human immunodeficiency virus (HIV) remain the leading killers of human beings worldwide, and function to destabilize societies in Africa, Asia, and the Middle East. Driven by the need to detect the presence of HIV viral sequence, here we demonstrate that the second-order nonlinear optical (NLO) properties of gold nanorods can be used for screening HIV-1 viral DNA sequence without any modification, with good sensitivity (100 pico-molar) and selectivity (single base-pair mismatch). The hyper-Rayleigh scattering (HRS) intensity increases 45 times when a label-free 145-mer, ss-gag gene DNA, was hybridized with 100 pM target DNA. The mechanism of HRS intensity change has been discussed with experimental evidence for higher multipolar contribution to the NLO response of gold nanorods.
Darbha
Gopala Krishna
GK
Department of Chemistry, Jackson State University, Jackson, MS, USA.
Rai
Uma Shanker
US
Singh
Anant Kumar
AK
Ray
Paresh Chandra
PC
D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D006046:Gold / Q000737:chemistry*; D015497:HIV-1 / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D015394:Molecular Structure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D013057:Spectrum Analysis
D004279:DNA, Viral; D006046:Gold