Chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system.
A non-motile, yellow-pigmented bacterium, designated strain CW9(T), was isolated from a water-cooling system in Gwangyang, Republic of Korea. The cells were Gram-negative, catalase- and oxidase-positive, short rods. The major fatty acids were iso-C(15 : 0) (45.5 %), iso-C(17 : 1)omega9c (14.4 %), iso-C(17 : 0) 3-OH (13.0 %) and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c; 8.8 %). The DNA G+C content was 35.6 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW9(T) formed a lineage within the genus Chryseobacterium and was closely related to Chryseobacterium gleum ATCC 35910(T) (98.4 % sequence similarity) and Chryseobacterium indologenes ATCC 29897(T) (97.8 % sequence similarity). Phenotypic characteristics and DNA-DNA relatedness data served to distinguish strain CW9(T) from these two species. On the basis of the evidence presented in this study, strain CW9(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquifrigidense sp. nov. is proposed. The type strain is CW9(T) (=KCTC 12894(T)=JCM 14756(T)).
Park
Seong Chan
SC
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea.
Kim
Mi Sun
MS
Baik
Keun Sik
KS
Kim
Eun Mi
EM
Rhee
Moon Soo
MS
Seong
Chi Nam
CN
D015373:Bacterial Typing Techniques; D001482:Base Composition; D045247:Chryseobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012034:Refrigeration; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S