Changes in the gene expression of C-myc and CD38 in HL-60 cells during differentiation induced by nicotinic acid-related compounds.
Changes in gene expression levels of c-myc and CD38 were examined during the differentiation of HL-60 cells to granulocytes due to three nicotinic acid-related compounds. CD38 expression was increased by isonicotinic acid and all-trans-retinoic acid (ATRA). Nicotinamide and nicotinamide N-oxide drastically decreased c-myc expression, but isonicotinic acid had no effect, suggesting that these compounds differentiate HL-60 to granulocytes through different pathways. These results should provide useful information as to the mechanisms of cell differentiation.
Ida
Chieri
C
Laboratory of Molecular and Cellular Biology, Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan.
Ogata
Shin
S
Okumura
Katsuzumi
K
Taguchi
Hiroshi
H
D051997:ADP-ribosyl Cyclase 1 / Q000032:analysis / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006098:Granulocytes / Q000166:cytology; D018922:HL-60 Cells; D006801:Humans; D022423:Myeloid Cells / Q000166:cytology*; D009539:Nicotinic Acids / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C489427:MYC protein, human; D009539:Nicotinic Acids; D016271:Proto-Oncogene Proteins c-myc; D051997:ADP-ribosyl Cyclase 1