title: Regulation of the smooth muscle contractile phenotype by nonmuscle myosin.
abstract: The contractile phenotype of a smooth muscle can broadly be classified as phasic or tonic. Following activation, phasic smooth muscle exhibits an initial period of rapid force activation, following which force falls to a lower steady state level. In contrast, force generated by tonic smooth muscle rises slowly to a sustained steady state. The differences in contractile patterns cannot be explained by the time course of either the Ca(2+) transient or phosphorylation of the 20-kDa regulatory myosin light chain (MLC(20)). Therefore, a molecular marker that defines tonic and phasic smooth muscle contractile properties remains elusive. Further, smooth muscle can maintain force at low levels of MLC(20) phosphorylation; often referred to as the latch state. The mechanism for the latch state is unknown and has been hypothesized to be due to a number of mechanisms including the formation of slowly cycling dephosphorylated or latch cross-bridges (Hai and Murphy, Am J Physiol 253:H1365-H1371, 1988). This review will focus evidence suggesting that nonmuscle myosin IIB (NMIIB) are the latch cross-bridges in smooth muscle and NMIIB content could define the tonic contractile phenotype.
authors.affiliation: Cardiovascular Diseases, Mayo Medical School, 200 1st Street SW, Rochester, MN 55905, USA.
authors.forename: Ozgur
authors.initials: O
authors.lastname: Ogut
authors.forename: Samantha L
authors.initials: SL
authors.lastname: Yuen
authors.forename: Frank V
authors.initials: FV
authors.lastname: Brozovich
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006801:Humans; D009119:Muscle Contraction / Q000502:physiology*; D009129:Muscle Tonus / Q000502:physiology; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism*; D018994:Myosin Light Chains / Q000378:metabolism*; D024501:Nonmuscle Myosin Type IIB / Q000378:metabolism*; D010641:Phenotype; D010766:Phosphorylation
chemical_list: D018994:Myosin Light Chains; D024501:Nonmuscle Myosin Type IIB; D002118:Calcium