Skeletal muscle Kv7 (KCNQ) channels in myoblast differentiation and proliferation.
Voltage-dependent K(+) channels (Kv) are involved in myocyte proliferation and differentiation by triggering changes in membrane potential and regulating cell volume. Since Kv7 channels may participate in these events, the purpose of this study was to investigate whether skeletal muscle Kv7.1 and Kv7.5 were involved during proliferation and myogenesis. Here we report that, while myotube formation did not regulate Kv7 channels, Kv7.5 was up-regulated during cell cycle progression. Although, Kv7.1 mRNA also increased during the G(1)-phase, pharmacological evidence mainly involves Kv7.5 in myoblast growth. Our results indicate that the cell cycle-dependent expression of Kv7.5 is involved in skeletal muscle cell proliferation.
Roura-Ferrer
Meritxell
M
Molecular Physiology Laboratory, Departament de Bioquímica i Biologia Molecular, Institut de Biomedicina, Universitat de Barcelona, Avda. Diagonal 645, E-08028 Barcelona, Spain.
Solé
Laura
L
Martínez-Mármol
Ramón
R
Villalonga
Núria
N
Felipe
Antonio
A
D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D051656:KCNQ Potassium Channels / Q000235:genetics / Q000378:metabolism*; D051657:KCNQ1 Potassium Channel / Q000235:genetics / Q000378:metabolism*; D024510:Muscle Development / Q000235:genetics; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000378:metabolism; D051381:Rats
D051656:KCNQ Potassium Channels; D051657:KCNQ1 Potassium Channel; C496911:Kcnq1 protein, rat; C501028:Kcnq5 protein, rat