[Optimization of water extraction condition of LiYan granule by Doehlert design].
OBJECTIVE
To study optimal water extraction condition of LiYan granule.


METHODS
HPLC methods were developed for the determination of irisfloremin in LiYan granule. The contents of irisfloremin and extraction rate were selected as assessment indices. Three factors were studied by Doehlert design, including water-adding amount, decoction time and times of decoction.


RESULTS
The optimal extracting condition was water consumed as 8 times of curd herb, and 3 times for 2 hours each time.


CONCLUSIONS
The water extraction process is feasible, and the experiment result can provide basic and instruction data for the ascertainment of water extraction process of LiYan granule.
Tao
Jian-fei
JF
Nanjing Medicial University, Nanjing 210029, China. lyptjfll@163.com
Chen
Li-na
LN
Du
Shu-hu
SH
D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012107:Research Design; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014867:Water
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012997:Solvents; C530396:liyan; D014867:Water