Association between the proliferative rate of neoplastic B cells, their maturation stage, and underlying cytogenetic abnormalities in B-cell chronic lymphoproliferative disorders: analysis of a series of 432 patients.
Limited knowledge exists about the impact of specific genetic abnormalities on the proliferation of neoplastic B cells from chronic lymphoproliferative disorders (B-CLPDs). Here we analyze the impact of cytogenetic abnormalities on the proliferation of neoplastic B cells in 432 B-CLPD patients, grouped according to diagnosis and site of sampling, versus their normal counterparts. Overall, proliferation of neoplastic B cells highly varied among the different B-CLPD subtypes, the greatest numbers of proliferating cells being identified in diffuse large B-cell lymphoma (DLBCL) and Burkitt lymphoma (BL). Compared with normal B cells, neoplastic B-CLPD cells showed significantly increased S + G(2)/M-phase values in mantle cell lymphoma (MCL), B-chronic lymphocytic leukemia (B-CLL), BL, and some DLBCL cases. Conversely, decreased proliferation was observed in follicular lymphoma, lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM), and some DLBCL patients; hairy cell leukemia, splenic marginal zone, and MALT-lymphoma patients showed S + G(2)/M phase values similar to normal mature B lymphocytes from LN. Interestingly, in B-CLL and MCL significantly higher percentages of S + G(2)/M cells were detected in BM versus PB and in LN versus BM and PB samples, respectively. In turn, presence of 14q32.3 gene rearrangements and DNA aneuploidy, was associated with a higher percentage of S + G(2)/M-phase cells among LPL/WM and B-CLL cases, respectively.
Quijano
Sandra
S
Servicio General de Citometría, Laboratorio de Hematología, Hospital Universitario Salamanca, Paseo de San Vicente, 58-182, 37007 Salamanca, Spain.
López
Antonio
A
Rasillo
Ana
A
Barrena
Susana
S
Luz Sánchez
Maria
M
Flores
Juan
J
Fernández
Carlos
C
Sayagués
José María
JM
Osuna
Carlos Salvador
CS
Fernández
Nuria
N
González
Marcos
M
Giraldo
Pilar
P
Giralt
Manuel
M
Pérez
Maria Carmen
MC
Martin-Antoran
José Manuel
JM
Gutiérrez
Oliver
O
Perdiguer
Luis
L
Díaz Mediavilla
Joaquín
J
González Silva
Manuel
M
Asensio Del Rio
Agustín
A
Cerveró
Carlos
C
Guerra
José Luis
JL
Butrón
Rosario
R
García
Maria del Carmen
Mdel C
Almeida
Julia
J
Orfao
Alberto
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001402:B-Lymphocytes / Q000473:pathology*; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged