[Determination of HPLC fingerprint of Qingfengtong capsule].
OBJECTIVE
To establish a fingerprint analysis method of Qingfengtong capsule by HPLC.


METHODS
The samples were extracted with 70% ethanol in an ultrasonic bath. The extracts were analyzed at 35 degrees C on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with 0.1 mol x L(-1) potassium dihydrogen phosphate water-solution as mobile phase A, and methanol as mobile phase B. The analysis followed a linear gradient elution program. Initial condition of the mobile phase was 10% B for 2 minutes; then changed to 90% B in 40 minutes. The flow rate was kept at 1.0 ml x min(-1) and the detector wavelength was 262 nm.


RESULTS
The main peaks in fingerprint chromatogram of Qingfengtong capsules were separated fairly well. The results of method validation meet the requirements for the fingerprints.


CONCLUSIONS
The established method can be used for the quality control of Qingfengtong capsules.
Zhang
Yong-Xin
YX
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Yang
Hua
H
Zhang
Qi-Wei
QW
Yi
Hong
H
Chen
Gui-Pin
GP
D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D034181:Epimedium / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D032841:Sinomenium / Q000737:chemistry
D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C539471:qingfengtong