Spectroelectrochemistry of P700 in native photosystem I particles and diethyl ether-treated thylakoid membranes from spinach and Thermosynechococcus elongatus.
The redox potentials (E(composite function')) of P700 in intact and diethyl ether-treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of +/-2-3 mV. Stepwise removal of antenna pigments by ether treatment caused distinct shifts of the E( composite function') value with increasing degree of water saturation in ether; negatively from +471 to +428 mV for spinach, but positively from +423 to +436 mV for T. elongatus. Such a contrasting behavior is discussed by invoking the mode of action of ether on the microenvironments around P700.
Zhang
Yanrong
Y
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Nakamura
Akimasa
A
Kuroiwa
Yoshinori
Y
Kato
Yuki
Y
Watanabe
Tadashi
T
D001426:Bacterial Proteins / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry*; D000458:Cyanobacteria / Q000737:chemistry* / Q000187:drug effects; D004563:Electrochemistry; D004986:Ether / Q000494:pharmacology*; D010084:Oxidation-Reduction; D045331:Photosystem I Protein Complex / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D013057:Spectrum Analysis; D018724:Spinacia oleracea / Q000737:chemistry* / Q000187:drug effects; D020524:Thylakoids / Q000737:chemistry* / Q000187:drug effects
D001426:Bacterial Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; D004986:Ether; D002734:Chlorophyll; C001785:chlorophyll P 700