title: Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection.
abstract: Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.
authors.affiliation: Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: Shankar
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Fnu
authors.initials: F
authors.lastname: Nagajyothi
authors.forename: Aparna
authors.initials: A
authors.lastname: Mukhopadhyay
authors.forename: Fabiana S
authors.initials: FS
authors.lastname: Machado
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Belbin
authors.forename: Antonio
authors.initials: A
authors.lastname: Campos de Carvalho
authors.forename: Fangxia
authors.initials: F
authors.lastname: Guan
authors.forename: Chris
authors.initials: C
authors.lastname: Albanese
authors.forename: Linda A
authors.initials: LA
authors.lastname: Jelicks
authors.forename: Michael P
authors.initials: MP
authors.lastname: Lisanti
authors.forename: Joao S
authors.initials: JS
authors.lastname: Silva
authors.forename: David C
authors.initials: DC
authors.lastname: Spray
authors.forename: Louis M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Herbert B
authors.initials: HB
authors.lastname: Tanowitz
mesh_terms: D000818:Animals; D002598:Chagas Cardiomyopathy / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014349:Trypanosoma cruzi / Q000502:physiology