title: [Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells].
abstract: OBJECTIVE
  To investigate the effect of quercetin (Que) on HEN1 cells, a kind of human nasopharyngeal carcinoma cells.
  
  
  METHODS
  Inhibition rate of quercetin on HEN1 was assayed by MTT method, cell apoptosis by flow cytometry (FCM), Caspase-3 expression of each group were determined by colorimetry set.
  
  
  RESULTS
  The inhibition of quercetin on HEN1 cells was shown in the dose-dependent (r = 0.709, P < 0.05) and time-dependent manner (r = 0.703, P < 0.01). The ratio of apoptotic and necrosis cells increased accompanied with added quercetin concentration. Cell cycle was specificly arrested inG2/M phase. Apoptosis cusp was revealed by FCM. While the activity of caspase-3 were also significantly upregulated in five groups after quercetin compared to control (P < 0.05).
  
  
  CONCLUSIONS
  Quercetin can activate the expression of Caspase-3 and induce the apoptosis of HEN1 cells through mitochondrion-depended pathway.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. lhkzhangfeng@163.com
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Cui
authors.forename: Ping
authors.initials: P
authors.lastname: Cao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D011794:Quercetin; C505407:CASP3 protein, human; D053148:Caspase 3