title: Direct immunofluorescence and immunohistochemistry in diagnostics of glomerulonephritis.
abstract: The needle biopsies from 60 transplanted and native kidneys have been processed and a prospective analysis of pattern, intensity and distribution of immunoglobulin deposits (IgA, IgG and IgM) and complement components (C3c and C1q) identified in these lesions has been carried out by immunohistochemistry with three step immunoperoxidase, in the period from 2000 to 2004. Those deposits were previously detected and analyzed by immunofluorescence. The samples consisted of 30 renal biopsies, previously diagnosed with glomerulonephritis and positive immunofluorescence and 30 renal biopsies without morphologic changes and deposits on immunofluorescence. 78,7% of the analyzed samples showed the identical results of the deposits of immunoglobulin and components of the complement with both, immunohistochemistry and immunofluorescence method. Sensitivity of the immunohistochemistry method with three step immunoperoxidase for all analyzed immunoglobulin and complement components is high (0,93), while specificity for the same method is 0,79. Standardized method of the three step immunoperoxidase on the paraffin embedded, formalin fixed needle renal biopsies could successfully replace the immunofluorescence method in diagnostic of GN, with the emphasis on a follow up and control of each single step in the procedure of the method.
authors.affiliation: Polyclinic for Laboratory Diagnostic University Clinical Centre Tuzla, Trnovac 1, 75000 Tuzla, Bosnia and Herzegovina.
authors.forename: Zinaida
authors.initials: Z
authors.lastname: Karasalihović
authors.forename: Ermina
authors.initials: E
authors.lastname: Iljazović
authors.forename: Dusan
authors.initials: D
authors.lastname: Ferluga
authors.forename: Elmir
authors.initials: E
authors.lastname: Cickusić
authors.forename: Jasminka
authors.initials: J
authors.lastname: Mustedanagić Mujanović
authors.forename: Jugoslav
authors.initials: J
authors.lastname: Stahov
authors.forename: Ina
authors.initials: I
authors.lastname: Skaljić
mesh_terms: D001707:Biopsy, Needle; D015922:Complement C1q / Q000378:metabolism; D015932:Complement C3c / Q000378:metabolism; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005921:Glomerulonephritis / Q000175:diagnosis* / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007668:Kidney / Q000276:immunology / Q000473:pathology; D011446:Prospective Studies; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015922:Complement C1q; D015932:Complement C3c