title: Fluorotelomer alcohols induce hepatic vitellogenin through activation of the estrogen receptor in male medaka (Oryzias latipes).
abstract: Here we report on the in vivo estrogenic effects of two fluorotelomer alcohols, such as 1H,1H,2H,2H-perfluorooctan-1-ol (6:2 FTOH) and 1H,1H,2H,2H-perfluorodecan-1-ol (8:2 FTOH), in male medaka (Oryzias latipes). An in vitro yeast two-hybrid assay indicated a significant, dose-dependent interaction between medaka estrogen receptor alpha (ERalpha) and coactivator TIF2 upon treatment with 6:2 FTOH, 8:2 FTOH or 2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-nonadecafluoro-1-decanol (NFDH). The relative ranks of tested chemicals on the estrogenic effects for medaka ERalpha descended in the order of estradiol-17beta (100)>>6:2 FTOH (0.16)>NFDH (0.016)>8:2 FTOH (0.0044). In contrast, no interaction with the ERalpha was observed upon treatment with perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorododecanoic acid (PFDA) or perfluoroundecanoic acid (PFUnDA). Expression analysis of hepatic vitellogenin (VTG) protein showed estrogenic potentials with, 6:2 FTOH and 8:2 FTOH, indicative of the induction of VTG synthesis in the livers of male medaka. We also investigated mRNA expression levels of two ER subtypes (ERalpha and beta) and two VTGs (VTG I and VTG II) in the livers of male medaka following exposure to FTOHs. Quantitative real-time polymerase chain reaction analyses revealed that hepatic ERalpha, VTG I, and VTG II mRNA responded rapidly to FTOHs such as 6:2 FTOH and 8:2 FTOH after 8-h exposure, whereas no effects of these compounds on ERbeta mRNA transcription were observed. These results from both in vitro and in vivo assays strongly suggest that certain FTOHs, such as 6:2 FTOH and 8:2 FTOH, induce hepatic VTG through activation of ERalpha in male medaka.
authors.affiliation: Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Kumamoto 862-8502, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ishibashi
authors.forename: Ryoko
authors.initials: R
authors.lastname: Yamauchi
authors.forename: Munekazu
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Joon-Woo
authors.initials: JW
authors.lastname: Kim
authors.forename: Masashi
authors.initials: M
authors.lastname: Hirano
authors.forename: Akemi
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Tominaga
authors.forename: Koji
authors.initials: K
authors.lastname: Arizono
mesh_terms: D000438:Alcohols / Q000633:toxicity*; D000818:Animals; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta / Q000235:genetics; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005466:Fluorocarbons / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009990:Oryzias / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014819:Vitellogenins / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000438:Alcohols; D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004968:Estrogens, Non-Steroidal; D005466:Fluorocarbons; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C442419:estrogen receptor alpha, medaka