title: [The influence of very-low-calorie diet on soluble adhesion molecules and their gene expression in adipose tissue of obese women].
abstract: BACKGROUND
  Adhesion molecules (AM) are proteins expressed on the endothelial surface that play an important role in development of endothelial dysfunction. Higher concentrations of AM were found in patients with atherosclerosis, obesity or type 2 diabetes mellitus. The aim of our study was to measure serum concentrations and gene expression of ICAM-1 (intercellular adhesion molecule 1), VCAM-1 (vascular adhesion molecule 1) and E-selectin in subcutaneous adipose tissue samples obtained by needle biopsy from obese women and healthy controls and to evaluate the effect of 3-weeks very-low-calorie diet (VLCD) on these parameters.
  
  
  RESULTS
  20 obese women (BMI 46.2 +/- 9.7 kg/m2) and 13 lean control women (BMI 23.8 +/- 2.3 kg/m2) were included into the study. Gene expression of AM in subcutaneous adipose tissue was measured using RT-PCR, serum AM levels were measured by multiplex immunoanalysis. At the baseline, serum E-selectin concentrations were higher in obese women compared to controls (24.4 +/- 2.3 vs. 15 +/- 1,5 ng/ml, p < 0,05). 3 weeks of VLCD significantly decreased BMI and serum E-selectin levels. Baseline mRNA expression of E-selectin, ICAM-1 and VCAM-1 in subcutaneous adipose tissue was lower in obese relative to lean women (p < 0.05). Weight reduction increased ICAM-1 and VCAM-1 gene expression (p < 0.05).
  
  
  CONCLUSIONS
  Our results suggest that the subcutaneous adipose tissue is not the major source of the studied soluble adhesion molecules in obese women and that the regulation of AM local gene expression in subcutaneous adipose tissue probably differs from its circulating levels.
authors.affiliation: III interní klinika a Ustav telovýchovného lékarství l. LF UK a VFN, Praha. lenkabosanska@centrum.cz
authors.forename: L
authors.initials: L
authors.lastname: Bosanská
authors.forename: Z
authors.initials: Z
authors.lastname: Lacinová
authors.forename: T
authors.initials: T
authors.lastname: Roubícek
authors.forename: M
authors.initials: M
authors.lastname: Mráz
authors.forename: M
authors.initials: M
authors.lastname: Bártlová
authors.forename: R
authors.initials: R
authors.lastname: Dolezalová
authors.forename: J
authors.initials: J
authors.lastname: Housová
authors.forename: J
authors.initials: J
authors.lastname: Kremen
authors.forename: D
authors.initials: D
authors.lastname: Haluzíková
authors.forename: M
authors.initials: M
authors.lastname: Matoulek
authors.forename: M
authors.initials: M
authors.lastname: Haluzík
mesh_terms: D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D004038:Diet, Reducing; D002149:Energy Intake; D005260:Female; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D015815:Cell Adhesion Molecules