A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1.
We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a new resource for the study of gene function by forward and reverse genetic approaches and constitutes a robust experimental data source for systems biology approaches.
de Berardinis
Véronique
V
Genoscope, Institut de Génomique (CEA), Evry, France. vberard@genoscope.fr
Vallenet
David
D
Castelli
Vanina
V
Besnard
Marielle
M
Pinet
Agnès
A
Cruaud
Corinne
C
Samair
Sumitta
S
Lechaplais
Christophe
C
Gyapay
Gabor
G
Richez
Céline
C
Durot
Maxime
M
Kreimeyer
Annett
A
Le Fèvre
François
F
Schächter
Vincent
V
Pezo
Valérie
V
Döring
Volker
V
Scarpelli
Claude
C
Médigue
Claudine
C
Cohen
Georges N
GN
Marlière
Philippe
P
Salanoubat
Marcel
M
Weissenbach
Jean
J
D000150:Acinetobacter / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D002244:Carbon / Q000378:metabolism; D002874:Chromosome Mapping; D003470:Culture Media; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008954:Models, Biological; D008957:Models, Genetic; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D049490:Systems Biology
D001426:Bacterial Proteins; D003470:Culture Media; D017931:DNA Primers; D002244:Carbon