title: Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant.
abstract: The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002 revealed an S3/S2 recombination event at the 3' end of the VP1 coding region. A recombination event resulted in the introduction of six Sabin 2 amino acid residues in a Sabin 3 genomic background. Furthermore, mutations associated with loss of the attenuated phenotype of Sabin 3 strains have been identified in the genome of isolate K/2002. The data presented here emphasize the need for careful planning of vaccination strategies, which involve stopping OPV administration in regions that are certified to be polio-free.
authors.affiliation: Department of Biochemistry and Biotechnology, University of Thessaly, Greece.
authors.forename: Evaggelos
authors.initials: E
authors.lastname: Dedepsidis
authors.forename: Vaia
authors.initials: V
authors.lastname: Pliaka
authors.forename: Zaharoula
authors.initials: Z
authors.lastname: Kyriakopoulou
authors.forename: Christos
authors.initials: C
authors.lastname: Brakoulias
authors.forename: Stamatina
authors.initials: S
authors.lastname: Levidiotou-Stefanou
authors.forename: Anastassia
authors.initials: A
authors.lastname: Pratti
authors.forename: Zissis
authors.initials: Z
authors.lastname: Mamuris
authors.forename: Panayotis
authors.initials: P
authors.lastname: Markoulatos
mesh_terms: D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D036022:Capsid Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D002648:Child; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D017955:Poliovirus / Q000737:chemistry / Q000235:genetics*; D011055:Poliovirus Vaccine, Oral / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D011995:Recombination, Genetic
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D011055:Poliovirus Vaccine, Oral; D011994:Recombinant Proteins