An easier method for locating replication origins based on real-time polymerase chain reaction.
Locating replication origins (ROs) is an old problem to which diverse answers have been previously given. However, a better and more convenient method still remains desirable because previously reported methods are time-consuming. In this study, we report an easier method for locating ROs based on real-time polymerase chain reaction. The feasibility of this method was demonstrated using Escherichia coli K-12 and Halobacterium salinarium NRC-1 as models. Our results show that this method is easy, fast, and accurate in locating ROs.
Mei
Y
Y
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Chen
D
D
Sun
D
D
Yang
Y
Y
Huang
Y
Y
Shen
P
P
Chen
X
X
D017931:DNA Primers / Q000032:analysis / Q000235:genetics; D019641:DNA, Archaeal / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D048168:Escherichia coli K12 / Q000235:genetics*; D019617:Halobacterium salinarum / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018741:Replication Origin; D012680:Sensitivity and Specificity
D017931:DNA Primers; D019641:DNA, Archaeal; D004269:DNA, Bacterial