Isolation of discrete nanoparticle-DNA conjugates for plasmonic applications.
Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (> or =20 nm) are difficult to purify by other means and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.
Claridge
Shelley A
SA
Department of Chemistry, Berkeley, CA 94720, USA.
Liang
Huiyang W
HW
Basu
S Roger
SR
Fréchet
Jean M J
JM
Alivisatos
A Paul
AP
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles
D004247:DNA