title: Crystallization and preliminary X-ray crystallographic analysis of rabbit L-gulonate 3-dehydrogenase.
abstract: Rabbit L-gulonate 3-dehydrogenase was crystallized using the oil-microbatch method at 295 K. X-ray diffraction data were collected to 1.70 A resolution from a crystal at 100 K using synchrotron radiation. The crystal belongs to the C-centred monoclinic space group C2, with unit-cell parameters a = 71.81, b = 69.08, c = 65.64 A, beta = 102.7 degrees. Assuming the presence of a monomeric protomer in the asymmetric unit gives a V(M) value of 2.21 A(3) Da(-1) and a solvent content of 44.4%. A cocrystal with NADH, which was isomorphous to the apo form, was also prepared and diffraction data were collected to 1.85 A resolution using Cu Kalpha radiation at 100 K.
authors.affiliation: Advanced Protein Crystallography Research Group, RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo-gun, Hyogo 679-5148, Japan.
authors.forename: Yukuhiko
authors.initials: Y
authors.lastname: Asada
authors.forename: Chizu
authors.initials: C
authors.lastname: Kuroishi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ukita
authors.forename: Rie
authors.initials: R
authors.lastname: Sumii
authors.forename: Satoshi
authors.initials: S
authors.lastname: Endo
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Matsunaga
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
authors.forename: Naoki
authors.initials: N
authors.lastname: Kunishima
mesh_terms: D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D011817:Rabbits
chemical_list: D002237:Carbohydrate Dehydrogenases; C020843:gulonate dehydrogenase