The effects of postnatal FSH substitution on Sertoli cell number and the sperm production capacity of the adult boar.
The role of FSH for Sertoli cell establishment and sperm production in the boar is not definitely known. In order to elucidate its function FSH was substituted postnatally in male pigs and the resulting effects on testicular histological traits and sperm production capacity were investigated when the boars had reached maturity. Six male piglets received pFSH from 18 to 48 days postnatally. Another six piglets instead received saline and served as controls. Blood samples were drawn to measure FSH, LH, testosterone and estradiol. After 28 weeks, the boars were trained to mount a dummy so that the spermatogenic capacity was tested by increasing the frequency of semen collection at the age of 30 weeks. Libido (latency time) and ejaculate criteria (volume, motility, morphological abnormalities) were determined. Thereafter the boars were killed and their testes analyzed for morphology, number of Sertoli cells, germ cells and Leydig cells as well as the ratio between mitosis and apoptosis in the tubules. FSH concentrations were twofold due to FSH application when compared to the controls. LH was low during the first 2 weeks of FSH treatment. Thereafter concentrations increased in three of the six treated animals but not in controls. Testosterone increased slightly over the application period both in the controls and the treated piglets. Estradiol levels were similar in both groups. Increased ejaculation frequency reduced sperm concentrations and sperm motility in all boars and the percentage of morphologically abnormal sperm increased. Ejaculate volumes and the time of latency were not significantly altered. No differences were observed between the controls and the FSH treated boars. The testicular parameters of both FSH- and control boars were identical for morphology, number of spermatogenic and somatic cells as well as mitosis-apoptosis equilibrium. The data demonstrated that a prolonged postnatal period of FSH concentrations does not influence the sperm production of the adult boar.
Wagner
A
A
Universität Hohenheim, Institut für Tierhaltung und Tierzüchtung, Fachgebiet Tierhaltung und Leistungsphysiologie (470A), Garbenstr. 17, 70599 Stuttgart, Germany.
Claus
R
R
D000818:Animals; D002452:Cell Count / Q000662:veterinary; D004542:Ejaculation / Q000187:drug effects / Q000502:physiology; D004958:Estradiol / Q000097:blood; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000494:pharmacology*; D006651:Histocytochemistry / Q000662:veterinary; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects*; D013091:Spermatogenesis / Q000187:drug effects* / Q000502:physiology; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018709:Statistics, Nonparametric; D013552:Swine / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D013739:Testosterone / Q000097:blood
D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone