title: Progressive ganglion cell degeneration precedes neuronal loss in a mouse model of glaucoma.
abstract: Glaucoma is characterized by retinal ganglion cell (RGC) pathology and a progressive loss of vision. Previous studies suggest RGC death is responsible for vision loss in glaucoma, yet evidence from other neurodegenerative diseases suggests axonal degeneration, in the absence of neuronal loss, can significantly affect neuronal function. To characterize RGC degeneration in the DBA/2 mouse model of glaucoma, we quantified RGCs in mice of various ages using neuronal-specific nuclear protein (NeuN) immunolabeling, retrograde labeling, and optic nerve axon counts. Surprisingly, the number of NeuN-labeled RGCs did not decline significantly until 18 months of age, at which time a significant decrease in RGC somal size was also observed. Axon dysfunction and degeneration occurred before loss of NeuN-positive RGCs, because significant declines in RGC number assayed by retrograde tracers and axon counts were observed at 13 months. To examine whether axonal dysfunction/degeneration affected gene expression in RGC axons or somas, NeuN and neurofilament-heavy (NF-H) immunolabeling was performed along with quantitative reverse transcription-PCR for RGC-specific genes in retinas of aged DBA/2 mice. Although these mice had similar numbers of NeuN-positive RGCs, the expression of neurofilament light, Brn-3b, and Sncg mRNA varied; this variation in RGC-specific gene expression was correlated with the appearance of NF-H immunoreactive RGC axons. Together, these data support a progression of RGC degeneration in this model of glaucoma, beginning with loss of retrograde label, where axon dysfunction and degeneration precede neuronal loss. This progression of degeneration suggests a need to examine the RGC axon as a locus of pathology in glaucoma.
authors.affiliation: Department of Neurological Surgery, University of Washington, Seattle, Washington 98104, USA.
authors.forename: Brian P
authors.initials: BP
authors.lastname: Buckingham
authors.forename: Denise M
authors.initials: DM
authors.lastname: Inman
authors.forename: Wendi
authors.initials: W
authors.lastname: Lambert
authors.forename: Ericka
authors.initials: E
authors.lastname: Oglesby
authors.forename: David J
authors.initials: DJ
authors.lastname: Calkins
authors.forename: Michael R
authors.initials: MR
authors.lastname: Steele
authors.forename: Monica L
authors.initials: ML
authors.lastname: Vetter
authors.forename: Nicholas
authors.initials: N
authors.lastname: Marsh-Armstrong
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Horner
mesh_terms: D000818:Animals; D002452:Cell Count / Q000379:methods; D016923:Cell Death / Q000502:physiology; D004195:Disease Models, Animal; D018450:Disease Progression; D005901:Glaucoma / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009410:Nerve Degeneration / Q000235:genetics / Q000473:pathology*; D009474:Neurons / Q000473:pathology* / Q000502:physiology; D012165:Retinal Ganglion Cells / Q000473:pathology* / Q000502:physiology