title: Class I integron-borne bla(VIM-1) carbapenemase in a strain of Enterobacter cloacae responsible for a case of fatal pneumonia.
abstract: We report here a fatal case of nosocomial pneumonia due to a multidrug-resistant (MDR) strain of Enterobacter cloacae, which was also resistant to carbapenems, in a patient with a diagnosis of Churg-Strauss syndrome. The microorganism was investigated for the presence of metallo- and extended-spectrum beta-lactamases. The strain produced two class 1 integrons carrying bla(VIM-1) and aadA2 gene cassettes located on chromosomal and plasmidic DNA, respectively, and a plasmid-encoded SHV-12 extended-spectrum beta-lactamase. Our results clearly demonstrate the transfer of this kind of resistance in E. cloacae with potentially serious clinical implications.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università degli Studi dell'Aquila, L'Aquila, Italy.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Mezzatesta
authors.forename: Marco
authors.initials: M
authors.lastname: Falcone
authors.forename: Cristina
authors.initials: C
authors.lastname: Pellegrini
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
authors.forename: Mario
authors.initials: M
authors.lastname: Venditti
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015267:Churg-Strauss Syndrome / Q000150:complications; D003428:Cross Infection / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D016972:Enterobacter cloacae / Q000201:enzymology*; D004756:Enterobacteriaceae Infections / Q000150:complications / Q000188:drug therapy / Q000382:microbiology*; D017809:Fatal Outcome; D006801:Humans; D032023:Integrons; D008297:Male; D008875:Middle Aged; D018410:Pneumonia, Bacterial / Q000150:complications / Q000188:drug therapy / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase