The protective efficacy against Schistosoma japonicum infection by immunization with DNA vaccine and levamisole as adjuvant in mice.
Levamisole (LMS) as an adjuvant enhances cell-mediated immunity in DNA vaccination; we investigated the efficacy and liver immunopathology alleviation of a DNA vaccine, VR1012-SjGST-32, in a LMS formulation in the murine challenge model. Compared to controls, the VR1012-SjGST-32 plus LMS can reduce worm and egg burdens, as well as, immunopathological complications associated chronic inflammation significantly in liver, which were apparently associated with Th1-type response. Together, these results suggest that the LMS as a potential Schistosome DNA vaccine adjuvant can enhance both worm killing and disease prevention, which is possibly mediated through the induction of a strong Th1-dominant environment in immunized mice.
Wang
Xiao
X
State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, 2 Yuanmingyuan Xi Road, Beijing 100094, China.
Jin
Huali
H
Du
Xiaogang
X
Cai
Chun
C
Yu
Yang
Y
Zhao
Gan
G
Su
Baowei
B
Huang
Shan
S
Hu
Yanxin
Y
Luo
Dongmei
D
She
Ruiping
R
Luo
Xinsong
X
Zeng
Xianfang
X
Yi
Xinyuan
X
Wang
Bin
B
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D007978:Levamisole / Q000008:administration & dosage*; D008099:Liver / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D019444:Vaccines, DNA; D007978:Levamisole