title: High-throughput, nontargeted metabolite fingerprinting using nominal mass flow injection electrospray mass spectrometry.
abstract: Flow injection electrospray-mass spectrometry (FIE-MS) is finding utility as a first-pass metabolite fingerprinting tool in many research fields. We provide a protocol that has proved reliable in large-scale research projects involving diverse sample matrices originating from plants, microbes and mammalian biofluids. Using Brachypodium leaf material as an example matrix all steps are summarized from sample extraction to data quality assessment. Alternative procedures for dealing with other common matrices such as bloods and urine are included. With little sample pretreatment, no chromatography and instrument cycle times of <5 min it is feasible to analyze >1,000 samples per week. Analysis of a typical batch of 240 samples (including first-pass data analysis) can be accomplished within 48 h.
authors.affiliation: Institute of Biological Sciences, Aberystwyth University, Aberystwyth, Ceredigion SY23 3DA, UK. meb@aber.ac.uk
authors.forename: Manfred
authors.initials: M
authors.lastname: Beckmann
authors.forename: David
authors.initials: D
authors.lastname: Parker
authors.forename: David P
authors.initials: DP
authors.lastname: Enot
authors.forename: Emilie
authors.initials: E
authors.lastname: Duval
authors.forename: John
authors.initials: J
authors.lastname: Draper
mesh_terms: D000818:Animals; D008660:Metabolism; D010936:Plant Extracts / Q000737:chemistry; D010944:Plants / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D010936:Plant Extracts