title: Pleiotropic effect of tatC mutation on metabolism of pathogen Yersinia enterocolitica.
abstract: OBJECTIVE
  To analyze the impact of depletion of the twin arginine translocation (TAT) system on virulence and physiology of Yersinia enterocolitica for a better understanding of its pathogenicity.
  
  
  METHODS
  We constructed a DeltatatC::SpR mutant of Yersinia enterocolitica by P1 phage mediated transduction using Escherichia coli K-12 DeltatatC::SpR strain as a donor.
  
  
  RESULTS
  A P1-mediated genetic material transfer was found between the two species of enterobacteria, indicating a great potential of acquisition of antibiotic resistance in emergency of a new threatening pathogen by genetic material exchanges. Periplasmic trimethylamine N-oxidase reductase activity was detected in the wild type Y. enterocolitica strain and translocation of this enzyme was completely abolished by the DeltatatC::SpR mutation. In addition, the DeltatatC::SpR mutation showed a pleiotropic effect on the metabolism of Y. enterocolitica. However, the tat mutation did not seem to affect the mobility and virulence of Y. enterocolitica under the conditions used.
  
  
  CONCLUSIONS
  Unlike other pathogenic bacteria studied, the TAT system of Y. enterocolitica might play an important role in the pathogenic process, which is distinct from other pathogens, such as Pseudomonas aeruginosa and enterohemorrhagic E. coli O157:H7.
authors.affiliation: Jiangsu Provincial Center for Diseases Control and Prevention, Nanjing 210009, Jiangsu, China.
authors.forename: Zhi-Yang
authors.initials: ZY
authors.lastname: Shi
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Ling
authors.initials: L
authors.lastname: Gu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Cui
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Wu
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
authors.forename: Bo
authors.initials: B
authors.lastname: Pang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Xu
authors.forename: Huai-Qi
authors.initials: HQ
authors.lastname: Jing
mesh_terms: D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D014161:Transduction, Genetic; D014774:Virulence; D015008:Yersinia enterocolitica / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors