title: [Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC].
abstract: OBJECTIVE
  To develop a HPLC method for simultaneous determination of the contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides.
  
  
  METHODS
  Chromatographic condition included a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) column and thmobile phase consisting of a mixture of methanol-0.2% phosphatic acid (7:93). The detection wavelength was at 205 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.
  
  
  RESULTS
  All calibration curves showed good linearity (r > or = 0.9997) within test ranges. The mean recoveries were 100.0%, 97.0% and 97.0% respectively.
  
  
  CONCLUSIONS
  This method is simple, rapid and accurate, and suitable for quality control of S. alopecuroides seed.
authors.affiliation: Xingjiang Institute of Chinese Material Medica and Ethica, Urumch 830002, China. gulnar_sabir@163.com
authors.forename: Gulnar
authors.initials: G
authors.lastname: Sabir
authors.forename: Haji Akber
authors.initials: HA
authors.lastname: Aisa
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Shi
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Jia
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D011807:Quinolizines; C046246:oxysophocarpine; C037573:oxymatrine; D000093842:Matrines