Listeria monocytogenes internalins bind to the human intestinal mucin MUC2.
Listeria monocytogenes cross the intestinal barrier causing systemic infections with high mortality rates. Intestinal infection triggers release of intestinal mucus. We show that three L. monocytogenes internalins, InlB, InlC and InlJ all bound to MUC2 (the major component of intestinal mucus), but not to the cell surface mucin MUC1. Binding was strongest to InlB>InlC>InlJ (P < 0.001). Listerial internalins are characterized by their internalin domain, composed by leucine rich repeats (LRR) followed by an immunogloblin-like region. We report here that the internalin domain of the InlJ protein also bound MUC2, suggesting that an internalin domain is sufficient to bind to MUC2.
Lindén
Sara K
SK
Mucosal Diseases Program, Mater Medical Research Institute, Level 3 Aubigny Place, Raymond Terrace, South Brisbane, QLD, 4101, Australia. slinden@mmri.mater.org.au
Bierne
Hélène
H
Sabet
Christophe
C
Png
Chin Wen
CW
Florin
Timothy H
TH
McGuckin
Michael A
MA
Cossart
Pascale
P
D001426:Bacterial Proteins / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000382:microbiology; D008089:Listeria monocytogenes / Q000378:metabolism*; D008088:Listeriosis / Q000382:microbiology; D008565:Membrane Proteins / Q000378:metabolism*; D018396:Mucin-1 / Q000378:metabolism; D055262:Mucin-2; D009077:Mucins / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs
D001426:Bacterial Proteins; C060192:MUC1 protein, human; C524625:MUC2 protein, human; D008565:Membrane Proteins; D018396:Mucin-1; D055262:Mucin-2; D009077:Mucins; C095768:inlB protein, Listeria monocytogenes; C103178:inlC protein, Listeria monocytogenes