title: Human cord blood cells can differentiate into retinal nerve cells.
abstract: Retinal degeneration and dystrophy are the major causes of blindness in the developed world. It has been reported that human cord blood cells (HCBCs) can differentiate into neuron-like cells in vitro. We have recently demonstrated that bone marrow cells (BMCs) of both mice and rats can differentiate into retinal nerve cells (RNCs). In the present study, we show the differentiation capacity of HCBCs into RNCs in vivo. We transplanted lineage-negative HCBCs into the subretinal space of severe combined immunodeficiency (SCID) mice. Two weeks after the transplantation, some of the transplanted cells expressed human nestin, human MAP2, human neuron specific enolase (NSE), beta-III tubulin and also rhodopsin. These results indicate that HCBCs can differentiate into RNCs and suggest that our new strategy could be used for the regeneration of retinal nerve cells in degenerative or dystrophic diseases.
authors.affiliation: First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka 570-8506, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Koike-Kiriyama
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Keizo
authors.initials: K
authors.lastname: Minamino
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Keiji
authors.initials: K
authors.lastname: Nakano
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Koike
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Yamada
authors.forename: Hiromi
authors.initials: H
authors.lastname: Mukaide
authors.forename: Akio
authors.initials: A
authors.lastname: Shigematsu
authors.forename: Tomomi
authors.initials: T
authors.lastname: Mizokami
authors.forename: Miyo
authors.initials: M
authors.lastname: Matsumura
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D036101:Cord Blood Stem Cell Transplantation; D005312:Fetal Blood / Q000166:cytology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D009475:Neurons, Afferent / Q000166:cytology*; D012160:Retina / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012243:Rhodopsin / Q000235:genetics
chemical_list: D015415:Biomarkers; D012243:Rhodopsin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases