High energy shock waves (HESW) increase paclitaxel efficacy in a syngeneic model of breast cancer.
The combined effect of high energy shock waves (HESW), generated by a piezoelectric device, and paclitaxel on Mat B-III rat breast cancer cells in vitro and in an in vivo animal model is presented. A significant reduction of in vitro Mat B-III cell proliferation versus cells treated with paclitaxel alone was observed with the combined exposure to paclitaxel (0.1, 1, or 10 nM) and HESW (0.22 mJ/mm2, 1000 shots). Moreover earlier induction and enhanced apoptosis occurred in cells subjected to the combined treatment with paclitaxel and HESW at 1 and 10 nM versus paclitaxel alone (p<0.01 and p<0.001, respectively). The percentage of apoptotic cells along with BAD mRNA expression, confirm a significant enhancement of apoptosis in tumor tissues subjected to the combined treatment with paclitaxel (2.5 mg/kg on days 7 and 11) and HESW (0.50 mJ/mm2, 500 shots on day 11) in comparison with paclitaxel alone. In conclusion, these data suggest that HESW enhance paclitaxel cytotoxicity in the Mat B-III syngeneic model of breast cancer.
Canaparo
Roberto
R
Department of Clinical Pathophysiology, University of Torino, Via Genova 3, Torino, Italy.
Serpe
Loredana
L
Zara
Gian Paolo
GP
Chiarle
Roberto
R
Berta
Laura
L
Frairia
Roberto
R
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000628:therapy*; D049109:Cell Proliferation / Q000187:drug effects; D003131:Combined Modality Therapy / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D019220:High-Energy Shock Waves / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling; D017239:Paclitaxel / Q000008:administration & dosage*; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051025:bcl-Associated Death Protein / Q000096:biosynthesis
D000970:Antineoplastic Agents; C494821:Bad protein, rat; D051025:bcl-Associated Death Protein; D017239:Paclitaxel