title: Structural and functional characterization of osmotically inducible protein C (OsmC) from Thermococcus kodakaraensis KOD1.
abstract: Osmotically inducible protein C (OsmC) is involved in the cellular defense mechanism against oxidative stress caused by exposure to hyperoxides or elevated osmolarity. OsmC was identified by two-dimensional electrophoresis (2DE) analysis as a protein that is overexpressed in response to osmotic stress, but not under heat and oxidative stress. Here, an OsmC gene from T. kodakaraensis KOD1 was cloned and expressed in Escherichia coli. TkOsmC showed a homotetrameric structure based on gel filtration and electron microscopic analyses. TkOsmC has a significant peroxidase activity toward both organic and inorganic peroxides in high, but not in low temperature.
authors.affiliation: Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Seong-Cheol
authors.initials: SC
authors.lastname: Park
authors.forename: Bang Phuong
authors.initials: BP
authors.lastname: Pham
authors.forename: Le
authors.initials: L
authors.lastname: Van Duyet
authors.forename: Baolei
authors.initials: B
authors.lastname: Jia
authors.forename: Sangmin
authors.initials: S
authors.lastname: Lee
authors.forename: Rui
authors.initials: R
authors.lastname: Yu
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Han
authors.forename: Jae-Kyung
authors.initials: JK
authors.lastname: Yang
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Hahm
authors.forename: Gang-Won
authors.initials: GW
authors.lastname: Cheong
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017386:Sequence Homology, Amino Acid; D019712:Thermococcus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D020122:tert-Butylhydroperoxide / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D003432:Cross-Linking Reagents; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D009195:Peroxidase