[Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells].
OBJECTIVE
To investigate the effect of quercetin (Que) on HEN1 cells, a kind of human nasopharyngeal carcinoma cells.


METHODS
Inhibition rate of quercetin on HEN1 was assayed by MTT method, cell apoptosis by flow cytometry (FCM), Caspase-3 expression of each group were determined by colorimetry set.


RESULTS
The inhibition of quercetin on HEN1 cells was shown in the dose-dependent (r = 0.709, P < 0.05) and time-dependent manner (r = 0.703, P < 0.01). The ratio of apoptotic and necrosis cells increased accompanied with added quercetin concentration. Cell cycle was specificly arrested inG2/M phase. Apoptosis cusp was revealed by FCM. While the activity of caspase-3 were also significantly upregulated in five groups after quercetin compared to control (P < 0.05).


CONCLUSIONS
Quercetin can activate the expression of Caspase-3 and induce the apoptosis of HEN1 cells through mitochondrion-depended pathway.
Zhang
Feng
F
Department of Otorhinolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. lhkzhangfeng@163.com
Cui
Yonghua
Y
Cao
Ping
P
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D011794:Quercetin / Q000494:pharmacology*
D011794:Quercetin; C505407:CASP3 protein, human; D053148:Caspase 3