[Correlation of -31G/C polymorphisms of survivin promoter to tumorigenesis of gastric carcinoma].
OBJECTIVE
Survivin, a bifunctional protein that regulates cell division and suppresses apoptosis, may play an important role in tumorigenesis. This study was to determine the correlations of survivin gene 31-GC polymorphisms to the occurrence of gastric carcinoma and survivin mRNA expression.


METHODS
The -31G/C single nucleotide polymorphism (SNP) of survivin promoter in peripheral blood samples from 96 gastric carcinoma patients and 67 healthy subjects was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing. Survivin mRNA expression in gastric carcinoma tissues was detected by real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR).


RESULTS
The genotype frequencies for -31G/G, -31G/C and -31C/C were 20.84%, 39.58% and 39.58% in gastric carcinoma patients, and 46.26%, 41.80% and 11.94% in healthy subjects, respectively. The frequencies of survivin-31C allele and C/C genotype were significantly higher in gastric carcinoma patients than in healthy subjects [59.37% vs. 32.84%, Chi2 = 22.26, P<0.005, odds ratio (OR)=2.98, 95% confidence interval (CI)=1.96-4.51; 39.58% vs. 11.94%, Chi2 =14.88, P<0.005, OR=4.83, 95% CI=2.91-8.03], but survivin mRNA was overexpressed with no significant difference in gastric cancer tissues subgrouped by genotypes.


CONCLUSIONS
The -31C genotype of survivin promoter is associated with gastric cancer, and may be a risk factor of gastric carcinoma.
Cheng
Zheng-Jiang
ZJ
Department of Clinical Laboratory Medicine, Xiangfan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Xiangfan, Hubei, 441021, P. R. China.
Hu
Li-Hua
LH
Huang
Shao-Jun
SJ
D005838:Genotype; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D013274:Stomach Neoplasms / Q000209:etiology / Q000235:genetics*; D000077022:Survivin; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D000077022:Survivin; D016159:Tumor Suppressor Protein p53