A CpG oligodeoxynucleotide acts as a potent adjuvant for inactivated rabies virus vaccine.
To develop a CpG containing oligodeoxynucleotide (CpG ODN)-enhanced rabies vaccine for stimulating an earlier production of rabies virus neutralizing antibody (RVNAb) with high titers, we designed a CpG ODN (BW006) and evaluated its adjuvant activities in enhancing the immune response to rabies vaccine with or without aluminum in mice. It was found that BW006 could facilitate the rabies vaccine to induce an earlier and more vigorous RVNAb response, resulting in more effective protection of mice from rabies virus challenge. In addition, three shots of rabies vaccine with BW006 induced compatible RVNAb level with that induced by five shots of aluminum-adjuvanted rabies vaccine. These data reveal that BW006 could be used as a promising adjuvant to replace of or combine with aluminum for developing more effective rabies vaccines.
Wang
Xueju
X
Department of Molecular Biology, College of Basic Medicine, Jilin University, Changchun 130021, China.
Bao
Musheng
M
Wan
Min
M
Wei
Hongfei
H
Wang
Li
L
Yu
Hongtao
H
Zhang
Xuesong
X
Yu
Yongli
Y
Wang
Liying
L
D000276:Adjuvants, Immunologic; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005260:Female; D007117:Immunization, Secondary / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D009838:Oligodeoxyribonucleotides; D011818:Rabies / Q000517:prevention & control; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology*; D016019:Survival Analysis
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; C408982:CPG-oligonucleotide; D009838:Oligodeoxyribonucleotides; D011819:Rabies Vaccines; C041524:aluminum sulfate