Lactic acid bacteria inhibit proinflammatory cytokine expression and bacterial glycosaminoglycan degradation activity in dextran sulfate sodium-induced colitic mice.
To evaluate the effect of lactic acid bacteria (LAB) in inflammatory bowel diseases (IBD), inhibitory effect of several LAB isolated from intestinal microflora and commercial probiotics against NO production of lipopolysaccharide (LPS)-stimulated RAW264.7 cells was measured and anti-inflammatory effect of NO production-inhibitory LAB, Lactobacillus plantarum HY115 and L. brevis HY7401, in dextran sulfate sodium (DSS)-induced experimental colitic mice was investigated. The oral administration of the LAB to mice inhibited colon shortening and myeloperoxidase productivity in DSS-induced colitic mice. These LABs repressed the mRNA expressions of IL-1beta, TNF-alpha and IFN-gamma, as well as the protein expressions of IL-1beta and IL-6 proteins in the colon. The activation of the transcription factor, NF-kB, induced by DSS, was also inhibited by LAB. The administration of LAB reduced the degradation activities of chondroitin sulfate and hyaluronic acid of intestinal bacteria, induced by DSS, of which could induce the cytotoxic metabolites against intestinal cells. These findings suggest that NO-inhibitory LAB against LPS-stimulated RAW264.7 cells may improve colitis by the regulation of the inflammatory cytokine expression via the activation of transcription factor NF-kB as well as GAGs-degrading intestinal microflora.
Lee
Hye-Sung
HS
Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Science, Kyung Hee University, 1, Hoegi, Dongdaemun-Ku, Seoul 130-701, Republic of Korea.
Han
Song-Yi
SY
Bae
Eun-Ah
EA
Huh
Chul-Sung
CS
Ahn
Young-Tae
YT
Lee
Jung-Hee
JH
Kim
Dong-Hyun
DH
D000818:Animals; D001644:Bifidobacterium / Q000502:physiology*; D003092:Colitis / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D003106:Colon / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016207:Cytokines / Q000378:metabolism*; D016264:Dextran Sulfate; D006025:Glycosaminoglycans / Q000378:metabolism*; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007778:Lactobacillus / Q000502:physiology*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019936:Probiotics; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D006025:Glycosaminoglycans; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma; D016264:Dextran Sulfate