Expression of podoplanin in the mouse salivary glands.
OBJECTIVE
Podoplanin is one of the most highly expressed lymphatic-specific genes. Here, we report the distribution of cells expressing podoplanin in mouse salivary glands.


METHODS
We immunohistochemically investigated the distribution of cells expressing podoplanin in mouse major salivary glands by laser-scanning microscopy. The expression of endothelial cell marker PECAM-1 was tested to discriminate lymphatic endothelium from salivary gland cells, and myoepithelial cells were identified by an antibody for P-cadherin.


RESULTS
The podoplanin expression was rarely found in acini of the parotid gland but clearly found at the basal portion of acini in the submandibular and sublingual glands. The number of portion reacted with anti-podoplanin is greater in the sublingual gland than in the submandibular gland. The expression was also found at the basal portion of ducts in all major salivary glands. The P-cadherin expression was rarely found in acini of the parotid gland but found in acini of the sublingual gland and on ducts in parotid and sublingual glands, corresponding to the area of podoplanin expression.


CONCLUSIONS
It was suggested that the acinar and myoepithelial cells in the salivary glands have the ability to express podoplanin, and that the expression may be concerned with the mucous saliva excretion.
Hata
Minoru
M
Department of Oral Growth & Development, Fukuoka Dental College, 2-15-1 Tamura, Fukuoka, 814-0193, Japan.
Ueki
Takeshi
T
Sato
Atsuko
A
Kojima
Hiroshi
H
Sawa
Yoshihiko
Y
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D012469:Salivary Glands / Q000378:metabolism*; D014059:Tongue / Q000378:metabolism / Q000473:pathology; D000098853:Podoplanin
D015820:Cadherins; D008562:Membrane Glycoproteins; C490441:Pdpn protein, mouse; D000098853:Podoplanin