Decreased gene expression of human beta-defensin-1 in the development of squamous cell carcinoma of the oral cavity.
The aim of this study was to investigate the gene expression of human beta-defensin-1, -2, -3 (hBD-1, -2, -3), interleukin-1beta, tumour necrosis factor-alpha and cyclooxygenase-2 in oral squamous cell carcinoma (OSCC) compared to benign and premalignant lesions as well as healthy controls. Biopsies of healthy gingiva (n=5), irritation fibroma (n=5), leukoplakia (n=5) and OSCC (n=5) were obtained during routine surgical procedures. RNA was extracted according to standard protocols and transcripts of hBD-1, -2, -3, interleukin-1beta, tumour necrosis factor-alpha and cyclooxygenase-2 were analysed by real-time polymerase chain reaction. The expression of hBD-1 was reduced in all lesions (5-fold in irritation fibroma and 2.5-fold in leukoplakia), but most significantly (50-fold) in OSCC. hBD-1 appears to play a role in the development of OSCC. The loss of its function might contribute to the malignant progression of these tumours.
Wenghoefer
M
M
University of Bonn, Uni-Klinikum Bonn Klinik and Poliklinik for MKG-Chirurgie, Bonn, Germany. m.wenghoefer@gmx.de
Pantelis
A
A
Dommisch
H
H
Reich
R
R
Martini
M
M
Allam
J-P
JP
Novak
N
N
Bergé
S
S
Jepsen
S
S
Winter
J
J
D000890:Anti-Infective Agents / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D051546:Cyclooxygenase 2 / Q000032:analysis; D018450:Disease Progression; D005350:Fibroma / Q000235:genetics / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005881:Gingiva / Q000033:anatomy & histology; D006801:Humans; D053583:Interleukin-1beta / Q000032:analysis; D007972:Leukoplakia, Oral / Q000235:genetics / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D011230:Precancerous Conditions / Q000235:genetics / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D023083:beta-Defensins / Q000032:analysis*
D000890:Anti-Infective Agents; C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; D051546:Cyclooxygenase 2