title: [Comparison of phylogeny analysis methods for rhizobia asolated from Albizia spp., Acacia spp. and Leucaena leucocephala].
abstract: Multilocus house-keeping gene sequence analysis is a hotspot of taxonomy and phylogeny of prokaryotes. In this research, we used atpD and gln II gene sequences to analyze the phylogeny of nine rhizobia strains of Albizia spp., Acacia spp. and Leucaena leucocephala and compared the results to that of 16S rDNA. The phylogenetic relationships based on the sequence analysis of these three genes were congruent at the genera level. CCBAU43060 and CCBAU 61139 were located in the branch of Rhizobium-Agrobacterium. CCBAU51471, CCBAU35220, CCBAU51276 and CCBAU61158 belonged to the genera of Mesorhizobium. CCBAU35234, CCBAU61178 and CCBAU35085 were assigned to Bradyrhizobium. Differences were found for some strains, for example CCBAU 61158, CCBAU43060, CCBAU61178, at the species level. Insertion fragment and mosaic gene were also found in some isolates. These results indicated that there was recombination between species in the same genera. It is reliable to determine the taxonomy status at genera levels based on the sequence analysis of 16S rDNA. If the relationships between strains belonging to the same genera were studied using the phylogeny methods, researches should be carried out with more than one house-keeping genes.
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou 450002, China. w_fengqin@163.com
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.forename: Yongfa
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Andong
authors.initials: A
authors.lastname: Song
authors.forename: Quanjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Wenxin
authors.initials: W
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002965:Classification / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S