[Immunogenic properties of recombinant vaccinia virus containing the human IL-2 gene].
A genetic construct of the human interleukin-2 (IL-2) gene within vaccinia virus (L-IVP strain) has been designed. The authors show the capacity of CV-1 cells infected with the recombinant vaccinia virus VV-SIL2 to secrete human IL-2 into the culture medium. Human IL-2 has been detected by immunoblotting. The sera from the animals immunized with the recombinant virus VV-SIL2 exhibited both human IL-2 and its antibodies throughout the observation period. This recombinant virus immunization induced both humoral and cell-mediated immune responses to human IL-2; the observed changes in the concentrations of cytokines are likely to suggest that the response predominantly followed a Th1 pathway. The study construct was nontoxic at the used concentrations and administration routes. The findings point that it is promising to investigate the adjuvant properties of the recombinant VV-SIL2 vaccine-based preparation for immunization in combination with various vaccines and to study this construct in therapy for cancer diseases.
Babkin
I V
IV
Riazankin
I A
IA
Lebedev
L R
LR
Bulychev
L E
LE
Nesterov
A E
AE
Nosareva
O V
OV
Babkina
I N
IN
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D016207:Cytokines / Q000097:blood; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D007279:Injections, Subcutaneous; D007376:Interleukin-2 / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011213:Poxviridae Infections / Q000097:blood* / Q000276:immunology*; D012900:Smallpox Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology*
D000914:Antibodies, Viral; D016207:Cytokines; D007376:Interleukin-2; D012900:Smallpox Vaccine; D014614:Vaccines, Synthetic