title: Involvement of aldolase A in X-ray resistance of human HeLa and UV(r)-1 cells.
abstract: To find novel proteins involved in radio-resistance of human cells, we searched for nuclear proteins, whose expression levels alter after X-ray irradiation in HeLa cells, using agarose fluorescent two-dimensional differential gel electrophoresis following mass spectrometry. We identified 6 proteins, whose levels were increased in nuclei 24h after irradiation at 5Gy, including aldolase A. Nuclear aldolase A levels increased twofold after the irradiation, however, total aldolase A levels did not change. When the expression of aldolase A was suppressed by its specific siRNA, sensitization of the suppressed cells to X-ray-induced cell death was observed. In addition, UV(r)-1 cells with higher aldolase A expression exhibited lower sensitivity to X-ray-induced cell death than the parental RSa cells with lower aldolase A expression. These results suggest that aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol.
authors.affiliation: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Mamoru
authors.initials: M
authors.lastname: Satoh
authors.forename: Shiping
authors.initials: S
authors.lastname: Chen
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tomonaga
authors.forename: Fumio
authors.initials: F
authors.lastname: Nomura
authors.forename: Nobuo
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D002467:Cell Nucleus / Q000201:enzymology / Q000528:radiation effects; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D040901:Proteomics; D034741:RNA, Small Interfering / Q000494:pharmacology; D011836:Radiation Tolerance / Q000187:drug effects / Q000235:genetics; D014965:X-Rays
chemical_list: D034741:RNA, Small Interfering; D005634:Fructose-Bisphosphate Aldolase