title: [Molecule character of rabies virus in 3 counties in Hunan China].
abstract: OBJECTIVE
  To study the rabies molecular biology features in animals between high incidence area of rabies and no rabies cases area in Hunan.
  
  
  METHODS
  detect saliva of dogs and brains of dogs and cats by direct immunofluorescence assay, review positive samples by RT-PCR, sequencing extract RNA virus for genetic analysis.
  
  
  RESULTS
  12 were detected rabies virus antigen and positive nucleoside acid in 82 dogs from Wugang city also 1 in 17 from Dongkou county; the positive rate: Wugang 14.63 percent, Dongkou 5.88 percent. No rabies virus was detected in 67 samples of dog brains from Fenghuang County. Also none in 28 samples of cat brains. Amplificating N gene of rabies virus from positive samples of dog brain's tissue (No Wg13, Dk13) by RT-PCR, it shows that homology of nucleoside acid between two strain of virus is 99.4 percent; also 99.1 percent of amino acid. The homology of nucleoside acid (amonio acid) among Wg13 stain and Chinese strain CTN and aG strain is 89.4 percent (98.2 percent) and 86.1 percent (95.1 percent); The homology of nucleoside acid (amonio acid) among Dk13 stain Chinese strain CTN and aG strain is 89.1 percent (98.0 percent), 86.1 percent (94.9 percent).Compare with isolated rabies virus from abroad, the homology between two strains and Indonesia is 92.8 percent and 93.2 percent, the most similar of them. The strains isolated from other countries including Japan, Sri Lanka and India are relatively lower; The sequence of gene Wg13 and Dk13 were taken replacement of amino acid.
  
  
  CONCLUSIONS
  Two strains are belong to type I rabies virus, comparing its N gene with current using vaccine strains, both are in same group, and homology are relatively higher.
authors.affiliation: Center for Disease Control Hunan Province, Changsha 410000, China.
authors.forename: Qi-you
authors.initials: QY
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Lv
authors.forename: Shu-bin
authors.initials: SB
authors.lastname: Luo
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhong
authors.forename: Cheng-long
authors.initials: CL
authors.lastname: Xiong
authors.forename: Xiang-di
authors.initials: XD
authors.lastname: Wang
authors.forename: An-bao
authors.initials: AB
authors.lastname: Yang
authors.forename: Yong-zhen
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002415:Cats; D004285:Dogs; D005814:Genes, Viral; D010802:Phylogeny; D011820:Rabies virus / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction