title: Activity of BAL 4815 against filamentous fungi.
abstract: OBJECTIVE
  BAL 4815 is a new antifungal drug and it is the active component of the antifungal triazole BAL 8557 (the water-soluble precursor). We studied the in vitro fungistatic and fungicidal activities of BAL 4815 against 103 clinical isolates of filamentous fungi, including 51 isolates of Aspergillus spp. and 52 isolates of non-Aspergillus filamentous fungi.
  
  
  METHODS
  We evaluated the in vitro activity of BAL 4815 against 51 isolates of Aspergillus spp., 20 isolates of dematiaceous fungi, 18 isolates of hyaline Hyphomycetes and 14 isolates of Zygomycetes. MICs were determined following the CLSI M38-A broth microdilution method, using RPMI 1640 medium buffered to pH 7.0 with MOPS. Microdilution plates were incubated at 35 degrees C and read at 24 and 48 h (Mucorales were read at 24 h). Minimal fungicidal concentrations were also determined.
  
  
  RESULTS
  For all isolates, geometric mean MICs, MIC(50)s, MIC(90)s and MIC ranges (mg/L) were: Aspergillus spp., 1.67, 2, 4 and 0.5-4; dematiaceous fungi, 1.62, 1, >8 and 0.03 to >8; hyaline Hyphomycetes, 2.41, 2, >8 and 0.03 to >8; and Zygomycetes, 6.81, 8, >8 and 0.03 to >8. Differences in susceptibility between genera were noted. Scedosporium prolificans, Fusarium spp., Mucor spp. and Rhizopus spp. (MIC(90) > 8 mg/L) were less susceptible than Aspergillus spp. (MIC(90) = 4 mg/L).
  
  
  CONCLUSIONS
  BAL 4815 has excellent in vitro activity against Aspergillus spp. and variable activity against other filamentous fungi.
authors.affiliation: Servicio de Microbiología y Parasitología, Hospital Universitario de Valme, Carretera de Cádiz s/n, Sevilla, Spain. cemarmar@gmail.com
authors.forename: C
authors.initials: C
authors.lastname: Martín de la Escalera
authors.forename: A I
authors.initials: AI
authors.lastname: Aller
authors.forename: E
authors.initials: E
authors.lastname: López-Oviedo
authors.forename: A
authors.initials: A
authors.lastname: Romero
authors.forename: A I
authors.initials: AI
authors.lastname: Martos
authors.forename: E
authors.initials: E
authors.lastname: Cantón
authors.forename: J
authors.initials: J
authors.lastname: Pemán
authors.forename: P
authors.initials: P
authors.lastname: García Martos
authors.forename: E
authors.initials: E
authors.lastname: Martín-Mazuelos
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D009570:Nitriles / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D011725:Pyridines
chemical_list: D000935:Antifungal Agents; D009570:Nitriles; D014230:Triazoles; C508735:isavuconazole; D011725:Pyridines