Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltica SH1T.
Rhodopirellula baltica SH1(T), which was isolated from the water column of the Kieler Bight, a bay in the southwestern Baltic Sea, is a marine aerobic, heterotrophic representative of the ubiquitous bacterial phylum Planctomycetes. We analyzed the R. baltica proteome by applying different preanalytical protein as well as peptide separation techniques (1-D and 2-DE, HPLC separation) prior to MS. That way, we could identify a total of 1115 nonredundant proteins from the intracellular proteome and from different cell wall protein fractions. With the contribution of 709 novel proteins resulting from this study, the current comprehensive R. baltica proteomic dataset consists of 1267 unique proteins (accounting for 17.3% of the total putative protein-coding ORFs), including 261 proteins with a predicted signal peptide. The identified proteins were functionally categorized using Clusters of Orthologous Groups (COGs), and their potential cellular locations were predicted by bioinformatic tools. A unique protein family that contains several YTV domains and is rich in cysteine and proline was found to be a component of the R. baltica proteinaceous cell wall. Based on this comprehensive proteome analysis a global schema of the major metabolic pathways of growing R. baltica cells was deduced.
Hieu
Cao Xuan
CX
Institute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald, Germany.
Voigt
Birgit
B
Albrecht
Dirk
D
Becher
Dörte
D
Lombardot
Thierry
T
Glöckner
Frank Oliver
FO
Amann
Rudolf
R
Hecker
Michael
M
Schweder
Thomas
T
D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D036103:Nanotechnology; D021382:Protein Sorting Signals; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D021382:Protein Sorting Signals; D020543:Proteome