title: Scanning conductance microscopy investigations on fixed human chromosomes.
abstract: Scanning conductance microscopy investigations were carried out in air on human chromosomes fixed on pre-fabricated SiO2 surfaces with a backgate. The point of the investigation was to estimate the dielectric constant of fixed human chromosomes in order to use it for microfluidic device optimization. The phase shift caused by the electrostatic forces, together with geometrical measurements of the atomic force microscopy (AFM) cantilever and the chromosomes were used to estimate a value for the dielectric constant of different human chromosomes.
authors.affiliation: MIC-Department of Micro and Nanotechnology, Technical University of Denmark, Lyngby, Denmark. chc@mic.dtu.dk
authors.forename: Casper Hyttel
authors.initials: CH
authors.lastname: Clausen
authors.forename: Jacob Moresco
authors.initials: JM
authors.lastname: Lange
authors.forename: Linda Boye
authors.initials: LB
authors.lastname: Jensen
authors.forename: Pranjul Jaykumar
authors.initials: PJ
authors.lastname: Shah
authors.forename: Maria Ioannou
authors.initials: MI
authors.lastname: Dimaki
authors.forename: Winnie Edith
authors.initials: WE
authors.lastname: Svendsen
mesh_terms: D002877:Chromosomes, Human / Q000737:chemistry*; D006801:Humans; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*