title: [Cloning of gene fragment of estrogen receptor-beta and its expression in mouse embryo].
abstract: In order to study the expression and regulation effects of estrogen receptor-beta (ERbeta) in the development of mouse embryo, the primer of ERbeta was designed, the ERbeta fragment was first obtained by RT-PCR and subcloned into plasmids pGEM- 3Z, then the recombinant plasmids were linearized with the restriction enzymes of EcoRand Hind. Using Sp6 and T7 RNA polymerase, the digoxigenin(dig) labeled sense and anti-sense probes were transcriped in vitro, respectively. Then the expression of ERbeta in mouse embryo was examined with the probes by whole-mount in situ hybridization. The results indicated that ERbeta is expressed in the brain, spinal neural tube, genital ridge, pericardium, limb bud and mandibular arch of 10.5 dpc embryo, and is also expressed in the telencephalon, mesencephalon, medulla oblongata, spinal cord and limb bud of 13.5 dpc embryo. These results suggest that ERbeta maybe play a role of regulation in sexual differentiation, primal differentiation of neural tube, further differentiation of three primary cerebral vesicles and spinal cord, generation and differentiation of bone and cartilage of limb bud, development of pericardium and configuration differentiation of mandibular in mouse embryo.
authors.affiliation: School of Life Science, Xuzhou Normal University, Xuzhou 221116, China. zhangzifengsuper@xznu.edu.cn
authors.forename: Zi-Feng
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Fan
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wu
authors.forename: Qun
authors.initials: Q
authors.lastname: Shan
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Yuan-Lin
authors.initials: YL
authors.lastname: Zheng
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004622:Embryo, Mammalian / Q000378:metabolism*; D047629:Estrogen Receptor beta / Q000235:genetics* / Q000502:physiology; D005260:Female; D017403:In Situ Hybridization; D051379:Mice; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D047629:Estrogen Receptor beta