title: [Effect of Mexican propolis extracts from Apis mellifera on Candida albicans in vitro growth].
abstract: Propolis is a resinous substance collected by bees (Apis mellifera) from different trees and bushes. Due to its antifungal, antibacterial, antiviral and antiparasitic properties, it has continued to be very popular throughout the time showing variable activity depending on its geographical origin. In Mexico, information about this product is very limited. The aim of this work was to evaluate the antifungal activity of four propolis ethanolic extracts from three different Mexican states, and four commercial extracts on Candida albicans growth. A reference strain (ATCC 10231) and 36 clinical isolates of C. albicans were used. The Minimal Inhibitory Concentration (MIC) was determined by the dilution on agar method. Growth curves on Sabouraud Dextrose broth with and without different propolis ethanolic extracts concentrations were performed. In addition, whether the effect was fungistatic or fungicide was determined. The propolis ethanolic extract obtained from Cuautitlán Izcalli, State of Mexico, showed the best biological activity, inhibiting 94.4% from the clinical isolates at 0.8 mg/ml; the reference strain was inhibited at 0.6 mg/ml. The propolis effect was fungistatic in low concentrations and fungicide in concentrations higher to MIC. The Mexican propolis ethanolic extract could be further investigated for its alternative use for the treatment of some C. albicans infections.
authors.affiliation: Facultad de Estudios Superiores Cuautitlán, Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, Mexico. marileqm@hotmail.com
authors.forename: María Leonor
authors.initials: ML
authors.lastname: Quintero-Mora
authors.forename: Amparo
authors.initials: A
authors.lastname: Londoño-Orozco
authors.forename: Francisca
authors.initials: F
authors.lastname: Hernández-Hernández
authors.forename: Patricia
authors.initials: P
authors.lastname: Manzano-Gayosso
authors.forename: Rubén
authors.initials: R
authors.lastname: López-Martínez
authors.forename: Carlos Ignacio
authors.initials: CI
authors.lastname: Soto-Zárate
authors.forename: Liborio
authors.initials: L
authors.lastname: Carrillo-Miranda
authors.forename: Guillermo
authors.initials: G
authors.lastname: Penieres-Carrillo
authors.forename: Carlos Gerardo
authors.initials: CG
authors.lastname: García-Tovar
authors.forename: Tonatiuh A
authors.initials: TA
authors.lastname: Cruz-Sánchez
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001516:Bees; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D045424:Complex Mixtures / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol; D066298:In Vitro Techniques; D008800:Mexico; D008826:Microbial Sensitivity Tests; D011429:Propolis / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D045424:Complex Mixtures; D000431:Ethanol; D011429:Propolis