High-resolution, high-throughput SNP mapping in Drosophila melanogaster.
Single nucleotide polymorphisms (SNPs) are useful markers for genetic mapping experiments in model organisms. Here we report the establishment of a high-density SNP map and high-throughput genotyping assays for Drosophila melanogaster. Our map comprises 27,367 SNPs in common laboratory Drosophila stocks. These SNPs were clustered within 2,238 amplifiable markers at an average density of 1 marker every 50.3 kb, or 6.3 genes. We have also constructed a set of 62 Drosophila stocks, each of which facilitates the generation of recombinants within a defined genetic interval of 1-2 Mb. For flexible, high-throughput SNP genotyping, we used fluorescent tag-array mini-sequencing (TAMS) assays. We designed and validated TAMS assays for 293 SNPs at an average resolution of 391.3 kb, and demonstrated the utility of these tools by rapidly mapping 14 mutations that disrupt embryonic muscle patterning. These resources enable high-resolution high-throughput genetic mapping in Drosophila.
Chen
Doris
D
Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Ahlford
Annika
A
Schnorrer
Frank
F
Kalchhauser
Irene
I
Fellner
Michaela
M
Viràgh
Erika
E
Kiss
Istvàn
I
Syvänen
Ann-Christine
AC
Dickson
Barry J
BJ
D000818:Animals; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D049750:Genome, Insect; D024510:Muscle Development / Q000235:genetics*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide