Fine-tuning of galactoglucan biosynthesis in Sinorhizobium meliloti by differential WggR (ExpG)-, PhoB-, and MucR-dependent regulation of two promoters.
Depending on the phosphate concentration encountered in the environment Sinorhizobium meliloti 2011 synthesizes two different exopolysaccharides (EPS). Galactoglucan (EPS II) is produced under phosphate starvation but also in the presence of extra copies of the transcriptional regulator WggR (ExpG) or as a consequence of a mutation in mucR. The galactoglucan biosynthesis gene cluster contains the operons wga (expA), wge (expE), wgd (expD), and wggR (expG). Two promoters, differentially controlled by WggR, PhoB, and MucR, were identified upstream of each of these operons. The proximal promoters of the wga, wge, and wgd transcription units were constitutively active when separated from the upstream regulatory sequences. Promoter activity studies and the positions of predicted PhoB and WggR binding sites suggested that the proximal promoters are cooperatively induced by PhoB and WggR. MucR was shown to strongly inhibit the distal promoters and bound to the DNA in the vicinity of the distal transcription start sites. An additional inhibitory effect on the distal promoter of the structural galactoglucan biosynthesis genes was identified as a new feature of WggR in a mucR mutant. A regulatory model of the fine-tuning of galactoglucan production is proposed.
Bahlawane
Christelle
C
Institute for Genome Research and Systems Biology, Center for Biotechnology, Bielefeld University, Universitätsstr. 25, 33594 Bielefeld, Germany.
Baumgarth
Birgit
B
Serrania
Javier
J
Rüberg
Silvia
S
Becker
Anke
A
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005685:Galactans / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005809:Genes, Regulator; D005936:Glucans / Q000378:metabolism*; D009876:Operon; D010710:Phosphates / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C100020:EXPG protein, Rhizobium meliloti; D005656:Fungal Proteins; D005685:Galactans; D005936:Glucans; C093770:MucR protein, Rhizobium meliloti; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D012097:Repressor Proteins; D015534:Trans-Activators; C061278:PhoB protein, Bacteria; C093768:galactoglucan