title: Epigenetics: application of virtual image restriction landmark genomic scanning (Vi-RLGS).
abstract: Restriction landmark genomic scanning (RLGS) is a powerful method for the systematic detection of genetic mutations in DNA length and epigenetic alteration due to DNA methylation. However, the identification of polymorphic spots is difficult because the resulting RLGS spots contain very little target DNA and many non-labeled DNA fragments. To overcome this, we developed a virtual image restriction landmark genomic scanning (Vi-RLGS) system to compare actual RLGS patterns with computer-simulated RLGS patterns (virtual RLGS patterns). Here, we demonstrate in detail the contents of the simulation program (rlgssim), based on the linear relationship between the reciprocal of mobility plotted against DNA fragment length and Vi-RLGS profiling of Arabidopsis thaliana.
authors.affiliation: Computational Astrophysics Laboratory, Discovery Research Institute, RIKEN, Saitama, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Koike
authors.forename: Tomoki
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Ebisuzaki
mesh_terms: D000818:Animals; D003198:Computer Simulation; D044127:Epigenesis, Genetic / Q000235:genetics*; D020869:Gene Expression Profiling; D005821:Genetic Techniques; D016678:Genome / Q000235:genetics*; D006801:Humans; D012984:Software