title: Fitness of transgenic Anopheles stephensi mosquitoes expressing the SM1 peptide under the control of a vitellogenin promoter.
abstract: Three transgenic Anopheles stephensi lines were established that strongly inhibit transmission of the mouse malaria parasite Plasmodium berghei. Fitness of the transgenic mosquitoes was assessed based on life table analysis and competition experiments between transgenic and wild-type mosquitoes. Life table analysis indicated low fitness load for the 2 single-insertion transgenic mosquito lines VD35 and VD26 and no load for the double-insertion transgenic mosquito line VD9. However, in cage experiments, where each of the 3 homozygous transgenic mosquitoes was mixed with nontransgenic mosquitoes, transgene frequency of all 3 lines decreased with time. Further experiments suggested that reduction of transgene frequency is a consequence of reduced mating success, reduced reproductive capacity, and/or insertional mutagenesis, rather than expression of the transgene itself. Thus, for transgenic mosquitoes released in the field to be effective in reducing malaria transmission, a driving mechanism will be required.
authors.affiliation: Johns Hopkins University, Bloomberg School of Public Health, Department of Molecular Microbiology and Immunology and Malaria Research Institute, 615 North Wolfe Street, Baltimore MD 21205, USA.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Li
authors.forename: Mauro T
authors.initials: MT
authors.lastname: Marrelli
authors.forename: Guiyun
authors.initials: G
authors.lastname: Yan
authors.forename: Marcelo
authors.initials: M
authors.lastname: Jacobs-Lorena
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000852:Anopheles / Q000235:genetics*; D005260:Female; D005298:Fertility; D005787:Gene Frequency; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012726:Sexual Behavior, Animal; D014170:Transformation, Genetic; D019076:Transgenes; D014819:Vitellogenins / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D010455:Peptides; D014819:Vitellogenins