title: Follicle-stimulating hormone receptor polymorphism (Thr307Ala) is associated with variable ovarian response and ovarian hyperstimulation syndrome in Indian women.
abstract: OBJECTIVE
  To evaluate the association of FSH receptor polymorphism and ovarian response.
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Academic research institute and private IVF clinic.
  
  
  METHODS
  Fifty women were recruited in an assisted reproductive technology program (ART) and 100 proven fertile women of Indian origin.
  
  
  METHODS
  Polymerase chain reaction, restriction fragment-length polymorphism for detecting polymorphisms at T(307)A and N(680)S.
  
  
  METHODS
  FSH receptor polymorphisms, serum FSH, and estradiol levels, amount of FSH administered, occurrence of ovarian hyperstimulation syndrome (OHSS).
  
  
  RESULTS
  Prevalence of polymorphism at 307 position was 24%, 53%, and 23% in controls and 24%, 62%, and 14% in ART subjects for TT, TA, and AA, respectively, whereas at position 680, it was 31%, 56%, and 13% in controls and 42%, 46%, and 12% in ART subjects for NN, NS, and SS, respectively. The amount of FSH required for ovulation induction was low in AA compared with TT and TA subjects; the estradiol levels before and on the day of hCG administration were significantly higher. Eighty-five percent of the subjects with AA genotype developed OHSS.
  
  
  CONCLUSIONS
  In Indian women, the subjects with AA genotype require low amounts of FSH for ovarian stimulation and have an increased risk of developing OHSS.
authors.affiliation: National Institute for Research in Reproductive Health, Mumbai, India.
authors.forename: Swati K
authors.initials: SK
authors.lastname: Achrekar
authors.forename: Deepak N
authors.initials: DN
authors.lastname: Modi
authors.forename: Sadhana K
authors.initials: SK
authors.lastname: Desai
authors.forename: Vijay S
authors.initials: VS
authors.lastname: Mangoli
authors.forename: Ranjana V
authors.initials: RV
authors.lastname: Mangoli
authors.forename: Smita D
authors.initials: SD
authors.lastname: Mahale
mesh_terms: D000328:Adult; D004252:DNA Mutational Analysis; D004958:Estradiol / Q000097:blood; D005260:Female; D005300:Fertility Agents, Female / Q000009:adverse effects*; D043373:Follicle Stimulating Hormone, Human / Q000009:adverse effects* / Q000097:blood; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India; D016017:Odds Ratio; D016471:Ovarian Hyperstimulation Syndrome / Q000097:blood / Q000139:chemically induced / Q000208:ethnology / Q000235:genetics*; D010060:Ovulation / Q000208:ethnology / Q000235:genetics*; D010062:Ovulation Induction / Q000009:adverse effects*; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011962:Receptors, FSH / Q000235:genetics*; D012189:Retrospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D044465:White People / Q000235:genetics*
chemical_list: D005300:Fertility Agents, Female; D043373:Follicle Stimulating Hormone, Human; D011962:Receptors, FSH; D004958:Estradiol