[Optimization of the gene composition of influenza H5 virus hemagglutinin-containing reassortants and their efficacy in immune cross-protection experiments].
The reassortant described in the authors' previous paper contained 6 genes originating from the high-yield virus A/Puerto Rico/8/34 (H1N1) and the genes of hemagglutinin (HA) and neuraminidase (NA) of the low-pathogenic avian influenza A/Duck/Primorie/2621/2001 (H5N2) (6:2 reassortant). The reassortant was used for the backcrossing with the parent avian virus in order to optimize the gene composition. Genotyping of the highest-yield second-generation reassortment indicated that it had obtained the PB1, HA, and NA genes from the virus A/Duck/Primorie/ 2621/2001 and the other genes received the genes from the virus A/Puerto Rico/8/34 (5:3 reassortant). The yield produced in the embryonated chicken eggs by the 5:3 reassortant was higher than that produced by the 6:2 reassortant although it did not achieve the reproduction of the parent virus A/Puerto Rico/8/34. Murine immunization with the inactivated reassortant containing the HA and NA genes of the virus A/Duck/Primorie/2621/2001 (H5N2) provided an efficient protection against the virus containing HA and NA of a recent H5N1 strain.
Rudneva
I A
IA
Kaverin
N V
NV
Timofeeva
T A
TA
Shilov
A A
AA
Varich
N L
NL
Kochergin-Nikitskiĭ
K S
KS
Krylov
P S
PS
L'vov
D K
DK
D000818:Animals; D006389:Hemagglutinins, Viral / Q000235:genetics; D007115:Immunization Schedule; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology; D053125:Influenza A Virus, H5N2 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007273:Injections, Intramuscular; D051379:Mice; D009439:Neuraminidase / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D016865:Reassortant Viruses / Q000276:immunology*; D014611:Vaccination; D014764:Viral Proteins / Q000235:genetics
D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D014764:Viral Proteins; D009439:Neuraminidase