Expression of HLA-G in the skin of patients with pemphigus vulgaris.
Studies on HLA-G, a nonpolymorphic antigen of non-classical HLA class I, is of basic and clinical significance. In the present study, the expression of HLA-G proteins in the human skin tissue sections of normal and autoimmune pemphigus vulgaris (PV) individuals were investigated using monoclonal antibodies. The antibodies recognized both membrane-bound and soluble isoforms of HLA-G. RT-PCR was performed to assess the patterns of HLA-G mRNA transcripts in the epidermal cells of PV and normal subjects. HLA-G expression could only be detected at transcriptional level in normal skin tissues. However cells derived from PV subjects expressed detectable HLA-G molecules at both transcriptional and translational levels. In addition, the RT-PCR patterns of HLA-G amplification revealed a reduction in HLA-G2 and an increase in HLA-G1 transcripts in epidermal cells of PV patients as compared to normal cells. These observations further support suggestions in the literature regarding the role of HLA-G in induction of tolerance in autoimmune individuals.
Yari
Fathemeh
F
Blood Transfusion Organization, Research Center, Tehran, Iran.
Zavaran Hosseini
Ahmad
A
Nemat Gorgani
Mohsen
M
Khorramizadeh
Mohammad Reza
MR
Mansouri
Parvin
P
Kazemnejad
Anoshirvan
A
D000911:Antibodies, Monoclonal; D018929:Cell Culture Techniques; D002675:Child, Preschool; D005260:Female; D052816:Foreskin / Q000276:immunology / Q000473:pathology; D005786:Gene Expression Regulation / Q000276:immunology*; D006680:HLA Antigens / Q000096:biosynthesis* / Q000276:immunology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D008856:Microscopy, Fluorescence; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I