High-fat diet may impair K(ATP) channels in vascular smooth muscle cells.
K(ATP) channel in vascular smooth muscle cell (VSMC) is closely linked to the etiology of hypertension. The aim of this study was to investigate effect of the high-fat diet-induced obesity on K(ATP) channel and blood pressure. Obesity was induced by a 24-week high-fat diet feeding in rats. Function and expression of K(ATP) channel in mesenteric arteries were examined using myography system, patch clamp and Western blotting. We show that high-fat diet increased blood pressure, decreased K(ATP) channel-mediated relaxation responses and currents, and down-regulated K(ATP) expression in VSMC. In conclusion, diet-induced obesity impairs K(ATP) channels in VSMC, which may underscore obesity-triggered increase in blood pressure.
Fan
Li-Hong
LH
Department of Cardiology, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, PR China.
Tian
Hong-Yan
HY
Yang
Miao-Li
ML
Ma
Ai-Qun
AQ
Hu
Zhi
Z
Bai
Xiao-Jun
XJ
Cao
Yong-Xiao
YX
D000818:Animals; D001794:Blood Pressure; D015153:Blotting, Western; D004041:Dietary Fats / Q000009:adverse effects*; D004195:Disease Models, Animal; D015536:Down-Regulation; D006973:Hypertension / Q000209:etiology; D054086:KATP Channels / Q000378:metabolism*; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D009213:Myography; D009765:Obesity / Q000209:etiology / Q000503:physiopathology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
D004041:Dietary Fats; D054086:KATP Channels