Protein levels and activity of some antioxidant enzymes in hippocampus of subjects with amnestic mild cognitive impairment.
Mild cognitive impairment (MCI) is generally referred to the transitional zone between normal cognitive aging and early dementia or clinically probable Alzheimer's disease (AD). Most individuals with amnestic MCI eventually develop AD, which suggests that MCI may be the earliest phase of AD. Oxidative stress is observed in brain from subjects with both AD and MCI. Among others, two possibilities for elevated oxidative stress are decreased activity or elevated expression of antioxidant enzymes, the latter as a response to the former. Accordingly, in the current study, the protein levels and activity of some antioxidant enzymes in the hippocampus of control and MCI brain were measured using Western blot analysis and spectrophotometric methods, respectively. Alterations in the levels and activity of a number of antioxidant enzymes in MCI brain compared to age-matched controls were found. These results are consistent with the hypothesis that oxidative stress may be an early event in the progression of amnestic MCI to AD.
Sultana
Rukhsana
R
Department of Chemistry, Center of Membrane Sciences, University of Kentucky, Lexington, KY 40506-0055, USA.
Piroddi
Marta
M
Galli
Francesco
F
Butterfield
D Allan
DA
D000647:Amnesia / Q000201:enzymology / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D003072:Cognition Disorders / Q000201:enzymology / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase