[Strategies for cloning of novel genes based on EST].
Expressed sequence tags (EST) are short, randomly selected single-pass nucleotide sequence reads derived from cDNA libraries and represent a small part of a gene. Along with the development of bioinformatics and genetic localization, EST has already become a powerful tool for mapping, cloning and expression profiling of genes. Recently, because of the fast distension of EST databases, application of EST in gene mapping and cloning leads to revolutionary change in the strategies for cloning of novel genes. Despite of some insufficiencies, it has been proved that EST could promote the discovery and research of novel genes. In this article, an introduction about EST, especially EST-based strategies for cloning of novel genes will be given in details.
Liu
Yuan
Y
Department of Pediatric Surgery, Huazhong University of Science and Technology, Wuhan 430022, China. liuliurachel@yahoo.com.cn
Cai
Jia-Bin
JB
Jiang
Guo-Song
GS
Tong
Qiang-Song
QS
D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D015723:Gene Library