title: Common marmoset embryonic stem cell can differentiate into cardiomyocytes.
abstract: Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.
authors.affiliation: Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Fumiyuki
authors.initials: F
authors.lastname: Hattori
authors.forename: Mitsushige
authors.initials: M
authors.lastname: Murata
authors.forename: Weizhen
authors.initials: W
authors.lastname: Li
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Yuasa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Onizuka
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Shimoji
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ohno
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.forename: Kensuke
authors.initials: K
authors.lastname: Kimura
authors.forename: Daihiko
authors.initials: D
authors.lastname: Hakuno
authors.forename: Motoaki
authors.initials: M
authors.lastname: Sano
authors.forename: Shinji
authors.initials: S
authors.lastname: Makino
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D000200:Action Potentials; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002144:Callithrix; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D011506:Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D011506:Proteins; D001973:Bromodeoxyuridine