title: Impact of occult HBV infection in HIV/HCV co-infected patients: HBV-DNA detection in liver specimens and in serum samples.
abstract: Prevalence and impact of occult HBV infection in HIV positive patients is controversial. The aims of this study were to determine the prevalence of occult HBV infection and its impact on histological and virological parameters. 52 HIV/HCV (but HBsAg-negative) co-infected patients, 29 HBsAg and anti-HCV negative chronic hepatitis, and 20 HBsAg positive chronic hepatitis controls were studied. DNA was extracted from frozen biopsies and amplified with primers for S, C and X regions, and for (ccc) HBV-DNA. Sera were tested for HBV-DNA with two quantitative assays (Cobas Amplicor HBV Monitor, and the real-time COBAS (r) Taqman HBV Test, Roche Diagnostics, UK). Occult HBV infection was detected in 7 (13.4%) liver biopsies of the study group, and in none case of the non viral chronic hepatitis group (p=0.04). All serum samples were HBV-DNA negative with Cobas Amplicor HBV monitor assay, while 3 cases were found positive with real time PCR. Statistical analysis didn't show any impact of occult HBV infection on liver histology, CD4+ cells count, HIV and HCV load, and ALT levels. Occult B infection is relatively frequent in HIV/HCV co-infected patients, and is underestimated by common HBV-DNA serological assays. However, it doesn't seem to exert a relevant impact.
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, S. Bortolo Hospital, Vicenza, Italy. pfabris@yahoo.com
authors.forename: Paolo
authors.initials: P
authors.lastname: Fabris
authors.forename: Maria R
authors.initials: MR
authors.lastname: Biasin
authors.forename: Maria T
authors.initials: MT
authors.lastname: Giordani
authors.forename: Laura
authors.initials: L
authors.lastname: Berardo
authors.forename: Vania
authors.initials: V
authors.lastname: Menini
authors.forename: Antonio
authors.initials: A
authors.lastname: Carlotto
authors.forename: Maria G
authors.initials: MG
authors.lastname: Miotti
authors.forename: Vinicio
authors.initials: V
authors.lastname: Manfrin
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Baldo
authors.forename: Gaia
authors.initials: G
authors.lastname: Nebbia
authors.forename: Domenico
authors.initials: D
authors.lastname: Infantolino
mesh_terms: D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D001706:Biopsy; D001769:Blood / Q000821:virology; D018791:CD4 Lymphocyte Count; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D015658:HIV Infections / Q000150:complications*; D015497:HIV-1 / Q000302:isolation & purification; D016174:Hepacivirus / Q000302:isolation & purification; D006509:Hepatitis B / Q000175:diagnosis* / Q000453:epidemiology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000302:isolation & purification*; D018937:Hepatitis C Antibodies / Q000097:blood; D019698:Hepatitis C, Chronic / Q000150:complications*; D006801:Humans; D008099:Liver / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D044967:Serum / Q000821:virology*; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D018937:Hepatitis C Antibodies; D000410:Alanine Transaminase