title: Gene expression in the rat brain during prostaglandin D2 and adenosinergically-induced sleep.
abstract: Previous studies have supported the hypothesis that macromolecular synthesis occurs in the brain during sleep as a response to prior waking activities and that prostaglandin D2 (PGD2) is an endogenous sleep substance whose effects are dependent on adenosine A2a receptor-mediated signaling. We compared gene expression in the cerebral cortex, basal forebrain, and hypothalamus during PGD2-induced and adenosinergically-induced sleep to results from our previously published study of recovery sleep (RS) after sleep deprivation (SD). Immediate early gene expression in the cortex during sleep induced by PGD2- or by the selective adenosine A2a agonist CGS21680 showed limited similarity to that observed during RS while, in the basal forebrain and hypothalamus, widespread activation of immediate early genes not seen during RS occurred. In all three brain regions, PGD2 and CGS21680 reduced the expression of arc, a transcript whose expression is elevated during SD. Using GeneChips, the majority of genes induced by either PGD2 or CGS21680 were induced by both, suggesting activation of the same pathways. However, gene expression induced in the brain after PGD2 or CGS21680 treatment was distinct from that described during RS after SD and apparently involves glial cell gene activation and signaling pathways in neural-immune interactions.
authors.affiliation: Biosciences Division, SRI International, Menlo Park, California 94025, USA.
authors.forename: Akira
authors.initials: A
authors.lastname: Terao
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Huang
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Wisor
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Dmitry
authors.initials: D
authors.lastname: Gerashchenko
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
authors.forename: T S
authors.initials: TS
authors.lastname: Kilduff
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D058908:Adenosine A2 Receptor Agonists; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D010627:Phenethylamines / Q000494:pharmacology; D015230:Prostaglandin D2 / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D043705:Receptor, Adenosine A2A / Q000502:physiology; D012890:Sleep / Q000187:drug effects / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: D058908:Adenosine A2 Receptor Agonists; D010627:Phenethylamines; D043705:Receptor, Adenosine A2A; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; D000241:Adenosine; D015230:Prostaglandin D2