title: Full genomic analysis of human rotavirus strain TB-Chen isolated in China.
abstract: A G2P[4]/NSP4[A] rotavirus strain TB-Chen was isolated from a 2-year-old patient hospitalized with acute gastroenteritis in Kunming, China. The strain TB-Chen was demonstrated having group A-specific antigenicity, a "short" (subgroup II) electropherotype. To investigate its overall genomic relatedness and to determine which group it belonged, the complete genome of strain TB-Chen was determined. Genomic comparison based on amino acid sequence identity and phylogenetic analysis revealed that all 11 gene segments of strain TB-Chen were highly identical (>91.80%) with the representative G2P[4]/NSP4[A] human strains DS-1, S2, NR1 and IS2, suggesting that this rotavirus strain was derived from human host. Besides, almost all the available representative rotavirus gene segments among group A were analyzed and identified within 15 G-types, 28 P-types, and 6 NSP4 genotypes. This is the first report of group A rotavirus genomic analyses in China and the findings have important implications for rotavirus vaccine development.
authors.affiliation: Key Laboratory, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China. chenyd@imbcams.com.cn
authors.forename: Yuanding
authors.initials: Y
authors.lastname: Chen
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wen
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Xinyu
authors.initials: X
authors.lastname: Xiong
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Cao
authors.forename: Qinghuan
authors.initials: Q
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Xingxiao
authors.initials: X
authors.lastname: Yin
authors.forename: Chuanyin
authors.initials: C
authors.lastname: Li
authors.forename: Yaochun
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000818:Animals; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology