Isolated lymphadenitis due to Histoplasma capsulatum diagnosed by fine-needle aspiration biopsy and immunohistochemistry.
In the disseminated form of histoplasmosis, isolation and further identification of Histoplasma capsulatum can be performed by several methods, namely, bone marrow aspiration, blood culture, and liver biopsy. Lymph node disease usually is diagnosed by excisional biopsy. Although fungal stains can identify this fungus, detection of specific antigens by immunohistochemistry shows a higher specificity and sensitivity. This approach can use the cell block method when the material is not sent to fungal cultures or fresh staining.
Tuon
Felipe F
FF
Department of Infectious Diseases, University of São Paulo, Medical School, São Paulo, Brazil. flptuon@gmail.com
Gomes
Vivian
V
Pagliari
Carla
C
Ctenas
Bruno B
BB
Carrasco Flores de Moscoso
Patrícia
P
Gerhard
René
R
Kanashiro
Luciane
L
Brasil
Roosecelis A
RA
Seixas Duarte
Maria Irma
MI
D000946:Antigens, Fungal / Q000032:analysis; D044963:Biopsy, Fine-Needle; D006658:Histoplasma / Q000276:immunology; D006660:Histoplasmosis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008199:Lymphadenitis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009333:Neck
D000946:Antigens, Fungal