Differential role of CD97 in interleukin-8-induced and granulocyte-colony stimulating factor-induced hematopoietic stem and progenitor cell mobilization.
CD97 is broadly expressed on hematopoietic cells and is involved in neutrophil migration. Since neutrophils are key regulators in HSC/HPC mobilization, we studied a possible role for CD97 in interleukin-8 and granulocyte-colony stimulating factor-induced HSC/HPC mobilization. Mobilization was absent in mice receiving CD97 mAb followed by interleukin-8, while granulocyte-colony stimulating factor-induced mobilization remained unaltered following anti-CD97 administration. Furthermore, combined administration of CD97 mAb and IL-8 induced a significant reduction in the neutrophilic compartment. We hypothesize that the absence of interleukin-8-induced HSC/HPC mobilization after CD97 mAb administration is due to its effect on neutrophil function.
van Pel
Melissa
M
Department of Immunohematology and Bloodtransfusion, Leiden University Medical Center, E3-Q, P.O. box 9600, 2300 RC Leiden, The Netherlands. m.van_pel@lumc.nl
Hagoort
Henny
H
Kwakkenbos
Mark J
MJ
Hamann
Jörg
J
Fibbe
Willem E
WE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D004334:Drug Administration Schedule; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D016209:Interleukin-8 / Q000008:administration & dosage / Q000494:pharmacology*; D007958:Leukocyte Count; D008297:Male; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000502:physiology; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000494:pharmacology
C490633:Adgre5 protein, mouse; D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D008562:Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D016179:Granulocyte Colony-Stimulating Factor