Antarctic marine molluscs do have an HSP70 heat shock response.
The success of any organism depends not only on niche adaptation but also the ability to survive environmental perturbation from homeostasis, a situation generically described as stress. Although species-specific mechanisms to combat "stress" have been described, the production of heat shock proteins (HSPs), such as HSP70, is universally described across all taxa. Members of the HSP70 gene family comprising the constitutive (HSC70) and inducible (HSP70) members, plus GRP78 (glucose-regulated protein, 78 kDa), a related HSP70 family member, were cloned using degenerate polymerase chain reaction (PCR) from two evolutionary divergent Antarctic marine molluscs (Laternula elliptica and Nacella concinna), a bivalve and a gastropod, respectively. The expression of the HSP70 family members was surveyed via quantitative PCR after an acute 2-h heat shock experiment. Both species demonstrated significant up-regulation of HSP70 gene expression in response to increased temperatures. However, the temperature level at which these responses were induced varied with the species (+6-8 degrees C for L. elliptica and +8-10 degrees C for N. concinna) compared to their natural environmental temperature). L. elliptica also showed tissue-specific expression of the genes under study. Previous work on Antarctic fish has shown that they lack the classical heat shock response, with the inducible form of HSP70 being permanently expressed with an expression not further induced under higher temperature regimes. This study shows that this is not the case for other Antarctic animals, with the two molluscs showing an inducible heat shock response, at a level probably set during their temperate evolutionary past.
Clark
Melody S
MS
British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge, UK. mscl@bas.ac.uk
Fraser
Keiron P P
KP
Peck
Lloyd S
LS
D000222:Adaptation, Physiological; D000818:Animals; D000864:Antarctic Regions; D049872:Bivalvia / Q000235:genetics / Q000502:physiology*; D000091342:Endoplasmic Reticulum Chaperone BiP; D049851:Gastropoda / Q000235:genetics / Q000502:physiology*; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D006358:Hot Temperature / Q000009:adverse effects*; D008386:Marine Biology; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012623:Seawater; D013045:Species Specificity; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger