Rapid capture of DNA targets.
A rapid capture technique was developed to efficiently isolate specific DNA targets from a variety of genomes. The specificity can be easily adapted to any target for which partial sequence is known, allowing for the isolation of a wide set of target molecules from either characterized or uncharacterized genomes. These targets include but are not limited to transposable elements, microsatellites, repetitive sequences, and possibly unique sequences. Additionally, because the thermodynamics of nucleic acid hybridizations differ from processes such as PCR, a wider variety of targets with a range of mismatches to any customized probe can be isolated. Further this method allows sequences flanking known internal regions to be co-isolated, facilitating the development of flanking primers for downstream applications. Considerable reduction in the frequency of nonspecific binding between key components (background) obviates the need for subsequent screening steps. Rapid capture of DNA targets quickly provides information about target and flanking sequences.
St John
Judy
J
Rocky Mountain Center for Conservation Genetics and Systematics, Department of Biological Sciences, University of Denver, Denver, CO 80208, USA. jstjohn@du.edu
Quinn
Thomas W
TW
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D008280:Magnetics; D008863:Microspheres; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D019809:Streptavidin / Q000378:metabolism; D013696:Temperature
D004247:DNA; D019809:Streptavidin