[Inhibitory effects of emodin on proliferation of rat vascular smooth muscle cell induced by angiotensin II].
OBJECTIVE
To investigate the effects of emodin on the proliferation of cultured rat vascular smooth muscle cell (VSMC) induced by angiotensin II.


METHODS
VSMCs were cultured by explant method. Cell proliferation model was established by stimulation with Ang II. Cell proliferation was measured by MTT assay to observe the effects of emodin (10, 20, 40 and 80 micromol x L(-1)) and N(G)-nitro-L-arginine methyl ester (L-NAME, 100 micromol x L(-1)) on VSMC proliferation induced by Ang II. The expression of PCNA was measured by immunohistochemical staining. Nitric oxide (NO) level was measured by Griess reagent. Nitric oxide synthase (NOS) and inducible nitric oxide synthase (iNOS) levels were detected by chemical colorimetric method. mRNA expression of iNOS was measured by reverse transcription polymerase chain reaction (RT-PCR).


RESULTS
Emodin at the doses range from 10 to 80 mol x L(-1) inhibited cell proliferation in a dose and time-dependent manner. The inhibitory effects were partly blocked by 100 mol x L(-1) of L-NAME. Emodin markedly decreased the expression of PCNA in VSMC, increased NO, NOS and iNOS levels, and increased iNOS mRNA expression in VSMC.


CONCLUSIONS
Emodin could inhibite VSMCs proliferation induced by Ang II. Inhibiting the expression of PCNA, increasing the NO secretion and upregulating the iNOS gene expression might be associated with the inhibitory effects.
Yang
Yu-Min
YM
Department of Cardiovascular Disease, First Affiliated Hospital, College of Medicine, ZheJiang University, Hangzhou 310003, China.
Wang
Shi-Jun
SJ
Jin
Dan-Dan
DD
Liu
Yun-Ying
YY
Wang
Xing-Xiang
XX
Chen
Jun-Zhu
JZ
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D004642:Emodin / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018809:Proliferating Cell Nuclear Antigen; D000804:Angiotensin II; C026512:arginine methyl ester; D009569:Nitric Oxide; D001120:Arginine; D052247:Nitric Oxide Synthase Type II; D004642:Emodin; D019331:NG-Nitroarginine Methyl Ester