An efficient and convenient synthesis of deuterium-labelled seminolipid isotopomers and their ESI-MS characterization.
Seminolipids 1a and 1b and galactosylalkylacylglycerols 2a and 2b, labelled with deuterium on the alkyl or acyl chain, respectively, were obtained isotopically and chemically pure through a straightforward synthesis from protected glycidyl galactoside 3 in an overall 22% yield. The identity and purity of compounds was ascertained by NMR spectroscopy and ESI mass spectrometry analysis. These labelled compounds are important as internal standards for quantification of these lipids by mass spectrometry, and they could also be used in metabolic studies in in vitro and even in vivo systems. Extension of the procedure could provide a route for the preparation of isotopomers of other compounds of the same general class.
Franchini
Laura
L
Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università di Milano, Via Saldini 50, 20133-Milano, Italy. franchini_laura@hotmail.com
Panza
Luigi
L
Kongmanas
Kessiri
K
Tanphaichitr
Nongnuj
N
Faull
Kym F
KF
Ronchetti
Fiamma
F
D000818:Animals; D002621:Chemistry / Q000379:methods; D003903:Deuterium / Q000737:chemistry*; D005260:Female; D006017:Glycolipids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008956:Models, Chemical; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013084:Sperm-Ovum Interactions; D013552:Swine; D013737:Testis / Q000378:metabolism
D006017:Glycolipids; D008055:Lipids; C011117:sulfoglycolipids; D003903:Deuterium