Determination of HLA-B27 subtypes in Iranian patients with ankylosing spondylitis.
The human leukocyte antigen-B27 is one of the class I molecules of the major histocompatibility complex which is strongly associated with ankylosing spondylitis (AS). The strength of the disease association with B27 varies markedly among racial and ethnic populations. It is an allele family, which constitutes about 31 subtypes, with a considerable geographic and ethnic difference in distribution. It is important to know whether certain subtypes show any preferential association with AS. Because there is no report regarding HLA-B27 subtypes in Iranian patients with AS, the main purpose of the present study was to assess the frequency of subtypes of human leukocyte antigen (HLA)-B27 in patients with ankylosing spondylitis in Iranian populationOne hundred and nineteen AS patients (82 HLA-B27 positive and 37 HLA-B27 negative) were selected for this study. HLA-B27 positive patients were screened by polymerase chain reaction amplification with sequence-specific primers (PCR-SSP) for B*27 subtyping.The results of present study revealed that only two subtypes were detected in Iranian patients, including B*2705 (52 patients, 63.4%) and B*2702 (30 patients, 36.6%). Our results showed a restricted number of HLA-B27 subtypes associated with AS in Iran and an elevated frequency of the B*2705 allele in these patients similar to other Euro-Caucasoid (Aryan) groups in the world.
Nicknam
Mohamad Hossein
MH
Molecular Immunology Research Center, Faculty of Medicine, Medical Sciences/ University of Tehran, Tehran, Iran. nicknam_m@yahoo.com
Mahmoudi
Mahdi
M
Amirzargar
Ali Akbar
AA
Ganjalikhani Hakemi
Mazdak
M
Khosravi
Farideh
F
Jamshidi
Ahmad Reza
AR
Amirkhani
Aref
A
Ansaripour
Bita
B
Pourpak
Zahra
Z
Moin
Mostafa
M
Nikbin
Behrooz
B
D000293:Adolescent; D000328:Adult; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D015235:HLA-B Antigens / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D015796:HLA-B27 Antigen / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D013167:Spondylitis, Ankylosing / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology
D015235:HLA-B Antigens; C464936:HLA-B*27:05 antigen; D015796:HLA-B27 Antigen