[Development and evaluation of a double antigen sandwich ELISA for the detection of total antibodies against hemorrhagic fever with renal syndrome virus].
OBJECTIVE
To develop and evaluate a method for detection of the total antibodies against hemorrhagic fever with renal syndrome (HFRS) virus with improved sensitivity and simplified operation procedure.


METHODS
The nucleic proteins of hantavirus were used as coating antigens as well as detection antigens labeled with horse radish peroxidase (HRP). The operation protocol was established, optimized and compared with indirect fluorescence assay (IFA).


RESULTS
The specificity of this method was 100 percent in the test of different human sera and 4-8 times more sensitive than IFA. And, it is simpler without requiring any change of the reagents, different sources of samples did not affect the results of the test.


CONCLUSIONS
This method is specific, sensitive and simple for detection of the total antibodies in sera against hantavirus, could be used for the screening of Hantavirus infection in human and host rodent animals.
Zhang
Quan-fu
QF
State Key Laboratory for Infectious Diseases, Institute for Viral Diseases Control and Prevention, Beijing 100052, China.
Li
Jian-dong
JD
Li
Wei-hong
WH
Li
Chuan
C
Liu
Qin-zhi
QZ
Liang
Mi-fang
MF
Li
De-xin
DX
D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018063:Hantaan virus / Q000276:immunology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral