[Simultaneous determination of neotame, alitame and aspartame in foods by HPLC].
Simultaneous determination of three artificial sweeteners, neotame (NE), alitame (AL) and aspartame (APM) in various foods by high-performance liquid chromatography (HPLC) was developed. Chopped or homogenized samples were packed into cellulose tubing with 0.01 mol/L hydrochloric acid containing 10% sodium chloride, and dialyzed against 0.01 mol/L hydrochloric acid for 24-48 hours. The dialyzate was passed through an Oasis MCX cartridge, and the cartridge was washed with water and methanol. Then the three sweeteners were eluted from the cartridge with a mixture of 0.5 mol/L ammonium chloride-acetonitrile (3 : 2). The sweeteners were separated on a Cosmosil 5C18-AR column using a gradient mode with a mobile phase of 0.01 mol/L phosphate buffer (pH 4.0)-acetonitrile and were detected at 210 nm. The recoveries of NE, AL and APM from 8 kinds of foods spiked with 10 and 100 microg/g were 86-100% and 89-104%, respectively. The detection limits of NE, AL and APM were 1 microg/g in samples. Furthermore, the three sweeteners were successfully identified by using liquid chromatography with tandem mass spectrometry.
Matsumoto
Hiroko
H
Tama Branch Institute, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Hirata
Keiko
K
Sakamaki
Narue
N
Hagino
Kayo
K
Ushiyama
Hirofumi
H
D001218:Aspartame / Q000032:analysis*; D002352:Carrier Proteins; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004151:Dipeptides / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D013549:Sweetening Agents / Q000032:analysis*
D002352:Carrier Proteins; D004151:Dipeptides; D013549:Sweetening Agents; C426003:ZFYVE1 protein, human; C082016:alitame; C404525:neotame; D001218:Aspartame