title: Rapid purification of annexin V from human placenta by affinity chromatography.
abstract: Affinity purification of annexin V from human placenta on column with appropriate monospecific antibodies is developed. The procedure permits purification of the protein to a highly purified state by a two stage procedure. The yield of the protein is about 5 mg per 100 g of wet tissue. Because of high homologies between various annexins, it was supposed that this procedure can be also applied for purification of other annexins from other tissues.
authors.affiliation: Institute of Biochemistry, National Academy of Sciences, Yerevan, Armenia.
authors.forename: M V
authors.initials: MV
authors.lastname: Mikaelyan
authors.forename: G G
authors.initials: GG
authors.lastname: Poghosyan
authors.forename: V K
authors.initials: VK
authors.lastname: Gasparyan
mesh_terms: D017304:Annexin A5 / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D010920:Placenta / Q000378:metabolism*
chemical_list: D017304:Annexin A5