Overexpression of yeast S-adenosylmethionine synthetase metK in Streptomyces actuosus leads to increased production of nosiheptide.
S-Adenosylmethionine (SAM) is synthesized via the metabolic reaction involving adenosine triphosphate and L-methionine that is catalyzed by the enzyme S-adenosyl-L-methionine synthetase (SAM-s) and encoded by the gene metK. In the present study, metK with the absence of introns from Saccharomyces cerevisiae was introduced into Streptomyces actuosus, a nosiheptide (Nsh) producer. Intracellular SAM levels were determined by high-pressure liquid chromatography. Through optimizing the nutrient content of the medium, it was shown that increased SAM production induced by the overexpression of SAM-s leads to an increase in the intracellular cysteine pool and overproduction of Nsh in S. actuosus. This investigation shows that increased SAM promotes the elevated production of the non-ribosomal thiopeptide Nsh in Streptomyces sp.
Zhang
Xincheng
X
School of Pharmacy, Fudan University, Shanghai, China.
Fen
Meiqing
M
Shi
Xunlong
X
Bai
Linquan
L
Zhou
Pei
P
D003545:Cysteine / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering; D007218:Industrial Microbiology; D008716:Methionine Adenosyltransferase / Q000235:genetics / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D029701:Saccharomyces cerevisiae Proteins; D013844:Thiazoles; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase; C014134:nosiheptide; D003545:Cysteine