Effects of repeated extracorporeal shock wave on kidney apoptosis of normal and diabetic rat.
OBJECTIVE
To assess the effect of repeated extracorporeal shock waves (ESW) on the apoptosis of renal parenchyma in normal and diabetic rats by the caspase-3 expression.


METHODS
80 rats were assigned for ESW (Direx Tripter X1(R) - 14 KVA) to one of three groups with 50% rats with diabetes (A) and 50% normal (B) in each: G1 (n = 40): no ESW; G2 (n = 20): one ESW; G3 (n = 20): two ESWin an interval of 14 days. The animals were sacrificed 3 days after the ESW and samples of the renal parenchyma were histologically prepared, stained by the caspase-3 cleavage immunohistochemical method, and the apoptotic index (ApIn) was calculated by computer program Image Pro-Plus V 4,5,1 Media Cybernetics ( ApIn = number of apoptotic cells in the microscopic section / total cells in the microscopic section).


RESULTS
There was no statistical difference in ApIn of normal rats with one ESW (8.4 +/- 0.2) or two ESW (9.1 +/- 0.3) and both were similar to the control group with no ESW (7.9 +/- 0.2). The ApIn of diabetic rats with one ESW (7.4 +/- 0.3) was similar to the normal rats with one or two ESW. On the other hand, the ApIn was statistically higher in diabetic rats with two ESW (11.9 +/- 0.4) than in diabetic rats with one ESW (7.4 +/- 0.3).


CONCLUSIONS
The ApIn of normal rats with a single (2,000 SW) or repeated ESW (4,000 SW) was similar to the control animals without any ESW. In diabetic rats, the repeated ESW (4,000 SW) may result in an additional risk of tissue damage expressed by the higher ApIn.
Kira
Vicente M
VM
Division of Urology and Division of Surgical Technique and Experimental Surgery, Department of Surgery, Paulista School of Medicine, Federal University of Sao Paulo, UNIFESP, Sao Paulo, SP, Brazil.
Fagundes
Djalma J
DJ
Bandeira
Cesar O P
CO
Kaufman
Oskar
O
Fagundes
Anna T N
AT
Ortiz
Valdemar
V
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D003928:Diabetic Nephropathies / Q000150:complications / Q000628:therapy; D004195:Disease Models, Animal; D004789:Enzyme Activation; D019220:High-Energy Shock Waves / Q000009:adverse effects*; D007668:Kidney / Q000098:blood supply / Q000293:injuries* / Q000473:pathology; D007669:Kidney Calculi / Q000628:therapy*; D008096:Lithotripsy / Q000009:adverse effects*; D008297:Male; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D053148:Caspase 3