Low generation polypropylenimine dendrimer graft beta-cyclodextrin: an efficient vector for gene delivery system.
The purpose of this study was to evaluate the characterization of a new cationic vector. Low generation polypropylenimine (DAB-8) was conjugated to beta-cyclodextrin (beta-CyD), and the chemical characters of the vector were investigated and confirmed by 1H NMR, FT-IR, TGA, TEM, particle size, and zeta potential assay. The biological property was identified by MTT assay and gene transfer efficiency was performed in cell lines. The results showed that the new vector had low cytotoxicity and high transfection efficiency in vitro. This suggested that beta-CyD-DAB-8 has a potential ability to act as a non-viral vector in gene delivery.
Zhang
Wei
W
Department of Gastroenterology, Institute of Clinical Medicine, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou.
Chen
Zhe
Z
Song
Xiaoxiao
X
Si
Jianmin
J
Tang
Guping
G
D000818:Animals; D002460:Cell Line; D050091:Dendrimers / Q000737:chemistry*; D005822:Genetic Vectors; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011126:Polypropylenes / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D050091:Dendrimers; D011126:Polypropylenes; D047392:beta-Cyclodextrins; C443641:poly(propyleneimine); C031215:betadex