CYP450 biosensors based on gold chips for antiepileptic drugs determination.
Three-electrode configuration chips containing a Pt, Au and a screen-printed Ag/AgCl as counter, working and reference electrode, respectively, have been developed. Selective determination of Phenobarbital (PB) has been carried out by Cytochrome P450 2B4 (CYP450) immobilization into a polypyrrole matrix onto the gold working electrode. Chronoamperometric experiments show a PB diffusion coefficient of 2.42x10(-6)cm(2)s(-1), a reproducibility and repeatability in terms of residual standard deviation (RSD) of 13% and 5.51%, respectively, and a limit of detection (LOD) of 0.289 micromol dm(-3) (alpha=beta=0.05) for the developed CYP450-biosensor chip. Its performance has been showed by the determination of PB in pharmaceutical drugs. HPLC has been used as reference technique.
Alonso-Lomillo
M A
MA
Department of Analytical Chemistry, Faculty of Sciences, University of Burgos, Burgos, Spain. malomillo@ubu.es
Gonzalo-Ruiz
J
J
Domínguez-Renedo
O
O
Muñoz
F J
FJ
Arcos-Martínez
M J
MJ
D000927:Anticonvulsants / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D008839:Microelectrodes; D010634:Phenobarbital / Q000032:analysis*
D000927:Anticonvulsants; D006046:Gold; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital