Quantification methods of folpet degradation products in plasma with HPLC-UV/DAD: application to an in vivo toxicokinetic study in rats.
Solid-phase extractions followed by HPLC-UV/DAD methods were developed for occupational biological monitoring or forensic investigations of the fungicide folpet using its degradation products, phthalimide and phthalamic acid as plasma biomarkers. These methods show good linearity (r>0.9955), precision (CV<15%) and accuracy (bias<14.8%). The lower limits of quantification for phthalimide and phthalamic acid were 10 and 20 ng/ml and the absolute recoveries were higher than 86% and 68%, respectively. Applying these methods, a plasma toxicokinetic study of folpet in rats after intratracheal administration of Folpan 80WG showed that inhalation of folpet could be a route of exposure with an important systemic absorption.
Canal-Raffin
Mireille
M
Departement de Pharmacologie, INSERM U657, Université Victor Segalen Bordeaux 2, 33076 Bordeaux, France. mireille.canal@pharmaco.u-bordeaux2.fr
Receveur
Mathilde
M
Martinez
Béatrice
B
Titier
Karine
K
Ohayon
Celine
C
Baldi
Isabelle
I
Molimard
Mathieu
M
Moore
Nicholas
N
Brochard
Patrick
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010797:Phthalimides / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D010797:Phthalimides; C005020:folpet