title: The Saccharomyces cerevisiae CLB5 promoter contains two middle sporulation elements (MSEs) that are differentially regulated during sporulation.
abstract: The B-type cyclins Clb5 and Clb6 are essential activators of DNA replication during sporulation in Saccharomyces cerevisiae. The expression of CLB5 is maximally induced during the middle phase of sporulation by the transcription factor Ndt80. We have performed an analysis of the CLB5 promoter and have identified two middle sporulation elements (MSEs) that act as binding sites for Ndt80. Although both MSE sequences bind Ndt80 in vitro, they display differential effectiveness in their ability to function as cis-acting regulatory sequences in vivo. Mutation of both MSE sequences in the CLB5 promoter profoundly reduces the induction of CLB5 transcription during the middle phase of sporulation but results in no obvious defect in progression through meiosis and sporulation, implying that the Ndt80-dependent induction of CLB5 is not required for effective DNA replication or chromosome division.
authors.affiliation: Department of Biochemistry, 561 Medical Sciences Building, University of Alberta, Edmonton, Alberta, T6G 2H7 Canada.
authors.forename: Sheetal A
authors.initials: SA
authors.lastname: Raithatha
authors.forename: David T
authors.initials: DT
authors.lastname: Stuart
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D019926:Cyclin B / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D020218:Response Elements; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D013172:Spores, Fungal / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: C076558:CLB5 protein, S cerevisiae; D019926:Cyclin B; D004268:DNA-Binding Proteins; C097071:NDT80 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors