Nucleotide insertion and bypass synthesis of pyrene- and BODIPY-modified oligonucleotides by DNA polymerases.
The chromophores pyrene and bordipyrromethenylbenzene directly linked to the 5-position of uridine are tolerated and recognized as thymine derivatives by DNA polymerases in primer extension experiments.
Wanninger-Weiss
Claudia
C
University of Regensburg, Institute for Organic Chemistry, D-93040 Regensburg, Germany.
Di Pasquale
Francesca
F
Ehrenschwender
Thomas
T
Marx
Andreas
A
Wagenknecht
Hans-Achim
HA
D001483:Base Sequence; D001896:Boron Compounds / Q000737:chemistry*; D002384:Catalysis; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014529:Uridine / Q000737:chemistry*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D009841:Oligonucleotides; D011721:Pyrenes; C030984:pyrene; D004259:DNA-Directed DNA Polymerase; D014529:Uridine