title: The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup.
abstract: OBJECTIVE
  A clone of CTX-M-15-producing Escherichia coli has recently been reported to be spreading through Europe and Africa. The aim of this work was to thoroughly characterize this clone.
  
  
  METHODS
  Representative isolates of this clone were subjected to multilocus sequence typing, O typing, virulence gene detection, adhesion assay on human cells, biofilm production assay and mouse lethality assay.
  
  
  RESULTS
  The clone: (i) belongs to a unique B2 phylogenetic subgroup encompassing the pyelonephritogenic diffusely adhering EC7372 strain; (ii) exhibits a specific O25b molecular subtype; (iii) is identical to the E. coli clone O25:H4-ST131 producing CTX-M-15; (iv) produces biofilm; and (v) is highly virulent in mice despite lacking classical extraintestinal pathogenicity islands (except for high pathogenicity island) and the afa/dra gene.
  
  
  CONCLUSIONS
  The CTX-M-15-producing E. coli diffusing clone is associated with a high level of antibiotic resistance and with high virulence, showing that, under certain selective pressures, the previously observed trade-off between resistance and virulence may not apply.
authors.affiliation: INSERM U722 and Université Denis Diderot-Paris 7, Faculté de Médecine, Site Xavier Bichat, Paris, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Clermont
authors.forename: Marie
authors.initials: M
authors.lastname: Lavollay
authors.forename: Sophie
authors.initials: S
authors.lastname: Vimont
authors.forename: Catherine
authors.initials: C
authors.lastname: Deschamps
authors.forename: Christiane
authors.initials: C
authors.lastname: Forestier
authors.forename: Catherine
authors.initials: C
authors.lastname: Branger
authors.forename: Erick
authors.initials: E
authors.lastname: Denamur
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arlet
mesh_terms: D002460:Cell Line; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010802:Phylogeny; D014774:Virulence; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases