Sequence analysis of the protein-coding regions of foot-and-mouth disease virus O/HK/2001.
The nucleotide sequence of the protein-coding region of foot-mouth-disease virus (FMDV) strain O/HK/2001 was determined and compared with the sequences of other FMDVs that were registered in GenBank. The protein-coding region was 6966 nucleotides in length and encoded a protein of 2322 amino acid residues. Comparison of the nucleotide sequence and its deduced amino acid sequence with those of other isolates indicated that O/HK/2001 belonged to the Cathay topotype. A genomic coding region nucleotide sequence phylogenetic tree of several FMDV-O isolates showed that O/HK/2001 was most closely related to FMDV isolates found in Taiwan during 1997, and especially shared significant similarity to HKN/2002, suggesting that the virus causing outbreaks in Hong Kong was genetically most-closely related to that causing an outbreak of type O in Taiwan. Mutations in O/HK/2001 were revealed, including frequent substitutions in the VP1 and L proteins, and deletions involving 10 amino acid residues in the 3A protein. This study was undertaken to assess the regional variation of prevalent FMDV type O viruses and to establish a sequence database for FMDV molecular epidemiological investigation.
Mingqiu
Zhao
Z
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510640, China.
Qingli
Suo
S
Jinding
Chen
C
Lijun
Chen
C
Yanfang
Xu
X
D000595:Amino Acid Sequence; D001483:Base Sequence; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
D012367:RNA, Viral; D017361:Viral Nonstructural Proteins