Comparative proteomics analysis of cerebrospinal fluid of patients with Guillain-Barré syndrome.
To better understand the pathophysiologic mechanisms underlying Guillain-Barré syndrome (GBS), Comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with GBS (the experiment group) and control subjects suffering from other neurological disorders (the control group) was carried out using two-dimensional gel electrophoresis (2-DE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and database searching to determine abnormal CSF proteins in GBS patients. Image analysis of 2-DE gels silver stained revealed that 10 protein spots showed significant differential expression between the two groups of CSF samples. The expression of cystatin C, transthyretin, apolipoprotein E and heat shock protein 70 were decreased. However, haptoglobin, alpha-1-antitrypsin, apolipoprotein A-IV and neurofilaments were elevated. The subsequent ELISA measured the concentration of cystatin C and confirmed the result of the proteomic analysis. These identified proteins may be involved in the pathophysiological process of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.
Yang
Yin-Rong
YR
Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44 Wenhuaxi Road, Jinan, Shandong, P.R. China.
Liu
Shi-Lian
SL
Qin
Zhao-Yu
ZY
Liu
Fu-Jun
FJ
Qin
Yan-Jiang
YJ
Bai
Shu-Mei
SM
Chen
Zhe-Yu
ZY
D000293:Adolescent; D000328:Adult; D001054:Apolipoproteins A / Q000032:analysis / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000032:analysis / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020275:Guillain-Barre Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D006242:Haptoglobins / Q000032:analysis / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000032:analysis / Q000134:cerebrospinal fluid; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods*; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000134:cerebrospinal fluid
D001054:Apolipoproteins A; D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D006242:Haptoglobins; D016900:Neurofilament Proteins; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin; C022205:apolipoprotein A-IV