A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensis.
Bacillus thuringiensis produces various groups of active proteins, such as Cyt, Vip and Parasporin, in addition to the Cry protein. In this study we show S-layer proteins to be a new group of parasporal inclusions of B. thuringiensis. The S-layer consists of a two-dimensional lattice structure and is the outermost component of many archaeobacteria and eubacteria. The parasporal inclusion of B. thuringiensis strain CTC was found to be not a typical crystal protein encoded by the cry gene, but a proteinaceous inclusion encoded by the S-layer gene. Furthermore, the CTC-like strains (with their parasporal inclusions coded by the S-layer gene) are widely distributed and accounted for 25.4% of the B. thuringiensis strains tested. These strains constitute a new group of parasporal inclusions encoded by the S-layer gene of B. thuringiensis and shed new light on B. thuringiensis nontoxic strains.
Guo
Gang
G
College of Life Science and Technology, Huazhong Agricultural University, China.
Zhang
Lei
L
Zhou
Zhou
Z
Ma
Qiqi
Q
Liu
Jianping
J
Zhu
Chenguang
C
Zhu
Lei
L
Yu
Ziniu
Z
Sun
Ming
M
D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D002479:Inclusion Bodies / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D007313:Insecta / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000254:growth & development
D004731:Endotoxins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; C412914:parasporin