title: Removal of bovine serum albumin using solid-phase extraction with in-situ polymerized stationary phase in a microfluidic device.
abstract: Serum albumin, one of the most abundant serum proteins, blocks the expression of other important biomarkers. The objective of this study is to remove serum albumin effectively by using solid-phase extraction (SPE) in microfluidic devices. Photo-polymerized adsorbent as a stationary phase of SPE was used to remove bovine serum albumin (BSA). The adsorption capacity was examined with the effect of pH and concentration in BSA solution, and adjustment of monomer concentration such as hydrophilic 2-acrylamido-2-methyl-1-propanesulfonic acid and acrylamide in the adsorbent. The effect of hydrophobic butyl methacylate on BSA adsorption was also studied. Selective removal in a bicomponent with BSA and bovine gamma-globulin was performed by adjusting the pH as required.
authors.affiliation: Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, South Korea.
authors.forename: Eun Zoo
authors.initials: EZ
authors.lastname: Lee
authors.forename: Yun Suk
authors.initials: YS
authors.lastname: Huh
authors.forename: Young-Si
authors.initials: YS
authors.lastname: Jun
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Won
authors.forename: Yeon Ki
authors.initials: YK
authors.lastname: Hong
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Won Hi
authors.initials: WH
authors.lastname: Hong
mesh_terms: D020106:Acrylamide / Q000528:radiation effects; D000178:Acrylamides / Q000528:radiation effects; D000327:Adsorption; D000476:Alkanesulfonates / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000528:radiation effects; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008855:Microscopy, Electron, Scanning; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000178:Acrylamides; D000476:Alkanesulfonates; D008689:Methacrylates; D020106:Acrylamide; D012710:Serum Albumin, Bovine; C056288:2-acrylamido-2-methylpropanesulfonate; C004919:ethylene dimethacrylate; C016284:butyl methacrylate