Effects of mycotoxins on mitogen-stimulated proliferation of bovine peripheral blood mononuclear cells.
The effects of mycotoxins on mitogen-stimulated proliferation of bovine peripheral blood mononuclear cells (PBMCs) were investigated. Aflatoxin B(1) (AFB(1)), deoxynivalenol (DON) and zearalenone (ZEN) were added to cultures of PBMCs, and the proliferation responses were measured using MTT bioassays. Suppression of the proliferation of calf PBMCs by AFB(1) and DON was significantly stronger than that of cow PBMCs, whereas there were no differences in suppressive effects on PBMCs from Holstein and Japanese Black calves and cows. The suppressive effect was greatest in the order of DON, AFB(1) and ZEN, and the effects of DON and AFB(1) seemed to be dose-dependent. The results suggest that some mycotoxins directly suppress proliferation of bovine PBMCs.
Wada
Kenji
K
Yamagata Prefecutural Federation of Agricultural Mutual Aid Association, Yamagata-shi, Yamagata, Japan. wada_k@yynosai.or.jp
Hashiba
Yayoi
Y
Ohtsuka
Hiromichi
H
Kohiruimaki
Masayuki
M
Masui
Machiko
M
Kawamura
Seiichi
S
Endo
Hiroshi
H
Ogata
Yoshimi
Y
D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects*; D008934:Mitogens / Q000494:pharmacology*; D009183:Mycotoxins / Q000494:pharmacology*
D008934:Mitogens; D009183:Mycotoxins