Quantitative detection of metallo-beta-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection.
In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase.
Motoshima
Maiko
M
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City, Nagasaki, Japan.
Yanagihara
Katsunori
K
Yamamoto
Kazuko
K
Morinaga
Yoshitomo
Y
Matsuda
Junichi
J
Sugahara
Kazuyuki
K
Hirakata
Yoichi
Y
Yamada
Yasuaki
Y
Kohno
Shigeru
S
Kamihira
Shimeru
S
D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000627:therapeutic use
D047090:beta-Lactams; D001618:beta-Lactamases; D027081:DNA Gyrase