Synergistic action of starch and honey against Aspergillus niger in correlation with Diastase Number.
To evaluate the synergistic action of starch on the antifungal activity of honey, a comparative method of adding honey with and without starch to culture media was used. Aspergillus niger was used to determine the minimum inhibitory concentration (MIC) of five varieties of honey. In the second step, lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to determine the minimum synergistic inhibitory concentration (MSIC). The MIC for the five varieties of honey without starch against A. niger ranged between 46% and 50% (v/v). When starch was incubated with honey and then added to media, an MIC drop was noticed with each variety and it ranged between 6% and 19.5%. Negative correlation has been established between the MIC drop and the Diastase Number.
Boukraâ
Laïd
L
Department of Veterinary Sciences, Faculty of Agro-Veterinary Sciences, Ibn-Khaldoun University of Tiaret, Tiaret, Algeria. laid_bouk@hotmail.com
Benbarek
Hama
H
Ahmed
Moussa
M
D000681:Amylases / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D004357:Drug Synergism; D006722:Honey / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D013213:Starch / Q000494:pharmacology*
D000935:Antifungal Agents; D003470:Culture Media; D013213:Starch; D000681:Amylases