title: Association between the proliferative rate of neoplastic B cells, their maturation stage, and underlying cytogenetic abnormalities in B-cell chronic lymphoproliferative disorders: analysis of a series of 432 patients.
abstract: Limited knowledge exists about the impact of specific genetic abnormalities on the proliferation of neoplastic B cells from chronic lymphoproliferative disorders (B-CLPDs). Here we analyze the impact of cytogenetic abnormalities on the proliferation of neoplastic B cells in 432 B-CLPD patients, grouped according to diagnosis and site of sampling, versus their normal counterparts. Overall, proliferation of neoplastic B cells highly varied among the different B-CLPD subtypes, the greatest numbers of proliferating cells being identified in diffuse large B-cell lymphoma (DLBCL) and Burkitt lymphoma (BL). Compared with normal B cells, neoplastic B-CLPD cells showed significantly increased S + G(2)/M-phase values in mantle cell lymphoma (MCL), B-chronic lymphocytic leukemia (B-CLL), BL, and some DLBCL cases. Conversely, decreased proliferation was observed in follicular lymphoma, lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM), and some DLBCL patients; hairy cell leukemia, splenic marginal zone, and MALT-lymphoma patients showed S + G(2)/M phase values similar to normal mature B lymphocytes from LN. Interestingly, in B-CLL and MCL significantly higher percentages of S + G(2)/M cells were detected in BM versus PB and in LN versus BM and PB samples, respectively. In turn, presence of 14q32.3 gene rearrangements and DNA aneuploidy, was associated with a higher percentage of S + G(2)/M-phase cells among LPL/WM and B-CLL cases, respectively.
authors.affiliation: Servicio General de Citometría, Laboratorio de Hematología, Hospital Universitario Salamanca, Paseo de San Vicente, 58-182, 37007 Salamanca, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Quijano
authors.forename: Antonio
authors.initials: A
authors.lastname: López
authors.forename: Ana
authors.initials: A
authors.lastname: Rasillo
authors.forename: Susana
authors.initials: S
authors.lastname: Barrena
authors.forename: Maria
authors.initials: M
authors.lastname: Luz Sánchez
authors.forename: Juan
authors.initials: J
authors.lastname: Flores
authors.forename: Carlos
authors.initials: C
authors.lastname: Fernández
authors.forename: José María
authors.initials: JM
authors.lastname: Sayagués
authors.forename: Carlos Salvador
authors.initials: CS
authors.lastname: Osuna
authors.forename: Nuria
authors.initials: N
authors.lastname: Fernández
authors.forename: Marcos
authors.initials: M
authors.lastname: González
authors.forename: Pilar
authors.initials: P
authors.lastname: Giraldo
authors.forename: Manuel
authors.initials: M
authors.lastname: Giralt
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Pérez
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Martin-Antoran
authors.forename: Oliver
authors.initials: O
authors.lastname: Gutiérrez
authors.forename: Luis
authors.initials: L
authors.lastname: Perdiguer
authors.forename: Joaquín
authors.initials: J
authors.lastname: Díaz Mediavilla
authors.forename: Manuel
authors.initials: M
authors.lastname: González Silva
authors.forename: Agustín
authors.initials: A
authors.lastname: Asensio Del Rio
authors.forename: Carlos
authors.initials: C
authors.lastname: Cerveró
authors.forename: José Luis
authors.initials: JL
authors.lastname: Guerra
authors.forename: Rosario
authors.initials: R
authors.lastname: Butrón
authors.forename: Maria del Carmen
authors.initials: Mdel C
authors.lastname: García
authors.forename: Julia
authors.initials: J
authors.lastname: Almeida
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D001402:B-Lymphocytes / Q000473:pathology*; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged