[Optimization of biotransformation conditions of active component in Panax notoginseng stalks and leaves by Fusarium sacchari].
By using Fusarium sacchari, a rare microbial strain isolated and screened from planted ginseng soil, the active component notoginseng triterpenes in Panax notoginseng stalks and leaves was biotransformed. Taking three main anti-tumor components, i. e., 20 (S)-protopanoxadiol-20-O-beta-D-glucopyranose (compound K, C-K), 20-O-beta-D-xylopyranosyl (1 --> 6) -beta-D-glucopyranosyl-20 (S)-protopanaxadiol (compound Mx, C-Mx) and 20 (S)-protopanoxadiol-20-O-alpha-L-arabofuranose (1 --> 6)-beta-D-glucopyranose (ginseng Mc, G-Mc) as evaluation indices, the optimization of biotransformation conditions of notoginseng triterpenes in P. notoginseng stalks and leaves were obtained by factor biotransformation experiment, i. e., initial pH value 6, substrate addition 40 mg, medium volume 30 ml, and transforming for 6 days at 30 degrees C. The method could increase the utility and economic benefit of P. notoginseng stalks and leaves effectively.
Han
Ying
Y
College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China. tase110@163.com
Hu
Xiao-Min
XM
Jiang
Bin-Hui
BH
Zhao
Yu-Qing
YQ
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000378:metabolism; D001711:Biotransformation; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D036145:Ginsenosides / Q000302:isolation & purification / Q000378:metabolism*; D005894:Panax / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology
D000972:Antineoplastic Agents, Phytogenic; D036145:Ginsenosides