The novel gene AngRem104 downregulates glucocorticoid receptor expression and activates NF-kappaB in human mesangial cells.
AngRem104 [angiotensin II (Ang II)-related genes in human mesangial cells (MCs), clone104], a novel gene in human MCs induced by Ang II, was previously identified in human MCs and found to interact with several proteins. The current study used a yeast two-hybrid system and co-immunoprecipitation to investigate the interaction between AngRem104 and glucocorticoid receptor (GR) AF-1-specific elongation factor (GR-EF). GR expression was downregulated and the number of MCs positive for activated nuclear factor kappaB (NF-kappaB) was increased when AngRem104 was overexpressed. Transfection with antisense AngRem104 vector resulted in the upregulation of GR protein and reduced numbers of MCs with activated NF-kappaB. These results indicate that the novel gene AngRem104 is involved in the in vivo regulation of GR expression and the activation of NF-kappaB through interaction with GR-EF in human MCs.
Zhang
Yanling
Y
Renal Division, Peking University First Hospital, Institute of Nephrology, Peking University, No. 8, Xishiku Street, Beijing 100034, China.
Zhang
Hong
H
Hou
Ping
P
Liang
Xiubin
X
Li
Zhuo
Z
Wang
Haiyan
H
D002478:Cells, Cultured; D015536:Down-Regulation; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
C094814:EEF1A1 protein, human; D016328:NF-kappa B; D009687:Nuclear Proteins; D020648:Peptide Elongation Factor 1; D011965:Receptors, Glucocorticoid