Gemella morbillorum in primary and secondary/persistent endodontic infections.
OBJECTIVE
To investigate the presence of Gemella morbillorum by culture or nested PCR in primary and secondary/persistent endodontic infections.


METHODS
Microbial samples were taken from 50 cases with primary and 50 cases with secondary/persistent endodontic infections. Microbiologic techniques were used for culture and identification. The DNA extracted from the samples was analyzed for the presence of the target species using species-specific primers.


RESULTS
Culture and polymerase chain reaction (PCR) identified the species in 23 and 77, respectively, of 100 root canals. Culture yielded the test organism in 19 of 50 (38%) of root canal samples from primary and in 4 of 50 (8%) from secondary/persistent infections. PCR yielded the test organisms in 41 of 50 (82%) and 36 of 50 (72%) of the, respectively, primary and secondary/persistent root canal infections studied.


CONCLUSIONS
Gemella morbillorum was identified more frequently in primary endodontic infections than in secondary/persistent ones. A higher frequency of the target species was detected by PCR than by culture.
Gomes
Brenda P F A
BP
Endodontic Division, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, Brazil. bpgomes@fop.unicamp.br
Montagner
Francisco
F
Jacinto
Rogério Castilho
RC
Pinheiro
Ericka Tavares
ET
Zaia
Alexandre A
AA
Ferraz
Caio Cezar Randi
CC
Souza-Filho
Francisco J
FJ
D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000032:analysis*; D003786:Dental Pulp Cavity / Q000382:microbiology*; D006801:Humans; D010482:Periapical Abscess / Q000382:microbiology*; D010485:Periapical Periodontitis / Q000382:microbiology*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012390:Root Canal Therapy / Q000379:methods; D040141:Staphylococcaceae / Q000302:isolation & purification*; D014098:Toothache / Q000209:etiology
D004269:DNA, Bacterial; D012388:Root Canal Irrigants