Veillonella rogosae sp. nov., an anaerobic, Gram-negative coccus isolated from dental plaque.
Strains of a novel anaerobic, Gram-negative coccus were isolated from the supra-gingival plaque of children. Independent strains from each of six subjects were shown, at a phenotypic level and based on 16S rRNA gene sequencing, to be members of the genus Veillonella. Analysis revealed that the six strains shared 99.7 % similarity in their 16S rRNA gene sequences and 99.0 % similarity in their rpoB gene sequences. The six novel strains formed a distinct group and could be clearly separated from recognized species of the genus Veillonella of human or animal origin. The novel strains exhibited 98 and 91 % similarity to partial 16S rRNA and rpoB gene sequences of Veillonella parvula ATCC 10790(T), the most closely related member of the genus. The six novel strains could be differentiated from recognized species of the genus Veillonella based on partial 16S rRNA and rpoB gene sequencing. The six novel strains are thus considered to represent a single novel species of the genus Veillonella, for which the name Veillonella rogosae sp. nov. is proposed. The type strain is CF100(T) (=CCUG 54233(T)=DSM 18960(T)).
Arif
Nausheen
N
Department of Microbiology, The Henry Wellcome Laboratories for Microbiology and Salivary Research, King's College London Dental Institute, London SE1 9RT, UK.
Do
Thuy
T
Byun
Roy
R
Sheehy
Evelyn
E
Clark
Douglas
D
Gilbert
Steven C
SC
Beighton
David
D
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology*; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014678:Veillonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit