title: The effects of aerobic exercise training on the age-related lipid peroxidation, Schwann cell apoptosis and ultrastructural changes in the sciatic nerve of rats.
abstract: The potential role of exercise in preventing the age-related spontaneous peripheral neuropathy has not been studied. We examined the effects of long-term aerobic exercise training on lipid peroxidation, Schwann cell (SC) apoptosis and ultrastructural changes in the sciatic nerve of rats during aging. Three groups of 12-week old Wistar rats ran on a treadmill for 6, 9 and 12 months (exercise trained (ET) group, n=10 each) according to an exercise training program targeted at a speed of 22 m/min (at 7 degrees incline), 60 min/day, 6 days/week. Three corresponding groups of untrained rats were used as the controls (sedentary (SED) group). At the end of each period, sciatic nerve biopsies were performed, and processed for biochemical, immunohistochemical and ultrastructural analyses. The results showed that aging was associated with an increased level of nerve malondialdehyde (MDA, marker of lipid peroxidation) and a higher number of SC apoptosis in SED group. The SED group showed irregular nerve fibers with thin myelin sheaths and areas of myelin-axon detachment. However, the ET group had significantly diminished nerve lipid peroxidation and SC apoptosis. In the ET group, nerve fibers had a thick myelin sheath with frequent folding. These findings suggest that aerobic exercise training protects peripheral nerves by attenuating oxidative reactions, and preserving SCs and myelin sheath from pathologic changes, which occur during normal aging.
authors.affiliation: Department of Neurosurgery, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Ghaffar
authors.initials: G
authors.lastname: Shokouhi
authors.forename: R Shane
authors.initials: RS
authors.lastname: Tubbs
authors.forename: Mohammadali M
authors.initials: MM
authors.lastname: Shoja
authors.forename: Leila
authors.initials: L
authors.lastname: Roshangar
authors.forename: Mehran
authors.initials: M
authors.lastname: Mesgari
authors.forename: Amir
authors.initials: A
authors.lastname: Ghorbanihaghjo
authors.forename: Naser
authors.initials: N
authors.lastname: Ahmadi
authors.forename: Farzam
authors.initials: F
authors.lastname: Sheikhzadeh
authors.forename: Jafar S
authors.initials: JS
authors.lastname: Rad
mesh_terms: D000332:Aerobiosis / Q000502:physiology*; D000375:Aging / Q000502:physiology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053938:DNA Fragmentation; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008854:Microscopy, Electron; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000502:physiology*; D012584:Sciatic Nerve / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D008315:Malondialdehyde