Diversity-oriented fluorescence library approach (DOFLA) to the discovery of chymotrypsin sensor.
The diversity-oriented fluorescence library approach (DOFLA) has emerged and found applications in various fields to meet the acute demands for novel fluorescence sensors. The power of this approach has been demonstrated with the impressive discoveries of novel sensors for polymers such as DNA and heparin or for small molecules such as GTP and glutathione ( J. Am. Chem. Soc. 2003, 125, 1130- 1131 ; J. Am. Chem. Soc. 2006, 128, 10380- 10381 ; J. Am. Chem. Soc. 2007, 129, 4510- 4511 ; Chem. Commun. [Online early access]. DOI: 10.1039/b717058k. Published online Dec 11, 2008. http://www.rsc.org/publishing/journals/CC/article.asp?doi=b717058k ). Herein we report the application of this approach on quinaldinium fluorescent dye library synthesis on solid support and novel chymotrypsin sensor discovery. The new sensors are not only selective to chymotrypsin over other proteins but also only to the active conformation of chymotrypsin.
Wang
Shenliang
S
Department of Chemistry, New York University, New York, New York 10003, USA.
Kim
Yun Kyung
YK
Chang
Young-Tae
YT
D015374:Biosensing Techniques; D002918:Chymotrypsin / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry*; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism
D005456:Fluorescent Dyes; D011804:Quinolines; D054852:Small Molecule Libraries; D002918:Chymotrypsin