title: Biofilm-forming associated genotypic and phenotypic characteristics of Staphylococcus spp. isolated from animals and air.
abstract: Biological monitoring is performed to detect and analyze microorganisms that have continuously made an effort to survive in the environment. Of such microorganisms, Staphylococcus spp. is considered a common cause of nosocomial and environmental infections., Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are required for the adhesion and biofilm formation of Staphylococci. Thirty-six and thirty-five Staphylococci isolated from animals and air, respectively, were analyzed. Biofilm formation and ten MSCRAMM genes were investigated using Congo red agar, tissue culture plate methods, and PCR. Airborne isolates were shown to have higher adherence and stronger biofilm formation than those from animals. The prevalence of MSCRAMM genes from air isolates was also higher than those from animals. Of the genes, eno was mainly associated with biofilm formation in both animals and airborne isolates (P<0.05). Moreover, the rate of airborne isolates harboring the eno gene was higher than in animal isolates. These results indicated that analysis of MSCRAMM genes with a phenotypic assay might be a helpful bacterial control system for the environment.
authors.affiliation: Department of Infectious Disease, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Yeon-Soo
authors.initials: YS
authors.lastname: Seo
authors.forename: Deog Yong
authors.initials: DY
authors.lastname: Lee
authors.forename: Nabin
authors.initials: N
authors.lastname: Rayamahji
authors.forename: Mi Lan
authors.initials: ML
authors.lastname: Kang
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000391:Air Microbiology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D003428:Cross Infection / Q000453:epidemiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D005838:Genotype; D010641:Phenotype; D013203:Staphylococcal Infections / Q000635:transmission / Q000662:veterinary; D013210:Staphylococcus / Q000235:genetics* / Q000302:isolation & purification*; D013552:Swine / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial