title: [Preventive and therapeutic effects of Bushen Huoxue Recipe on autoimmune premature ovarian failure in mice].
abstract: OBJECTIVE
  To investigate the preventive effect of Bushen Huoxue Recipe (BSHXR), a compound traditional Chinese herbal medicine, on autoimmune premature ovarian failure (POF) in mice.
  
  
  METHODS
  Ovarian antigen was prepared with the ovarian tissue of female BALB/c mice. A mouse model of POF was established by immunization injection of the ovarian antigen of isotype female mice on multiple subcutaneous sites and two posterior soles. The POF mice were treated with BSHXR after the first and third immunization. The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in peripheral blood were detected by enzyme linked immunosorbent assay.
  
  
  RESULTS
  Lymphocyte infiltration was appeared in ovarian stroma of POF mice. The levels of FSH and LH were evaluated and the E(2) level was decreased significantly (P<0.05). BSHXR could reduce the increased levels of FSH and LH, increase the level of E(2) and the number of growing and mature follicles. The efficacy of early treatment was better than that of late treatment.
  
  
  CONCLUSIONS
  BSHXR can recover ovarian function in POF mice mainly by regulating the indiscriminate hormone level, and BSHXR has preventive effect on autoimmune POF in mice.
authors.affiliation: Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. dongli888@yahoo.com
authors.forename: Li
authors.initials: L
authors.lastname: Dong
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Men
authors.forename: Nan-sun
authors.initials: NS
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000188:drug therapy / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000188:drug therapy / Q000517:prevention & control*
chemical_list: D004365:Drugs, Chinese Herbal; C527851:bushen huoxue; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone