Relation between lipid peroxidation and iron concentration in mouse liver after acute and subacute cadmium intoxication.
In this study the effect of acute and subacute cadmium (Cd) intoxication on iron (Fe) concentration and lipid peroxidation (LPO) was investigated in the livers of Swiss mice. Animals were divided into two groups: the Cd group--mice intoxicated with Cd and controls. In acute time-response studies, Fe and malondialdehyde (MDA) levels were determined at 4, 6, 12, 24 and 48 h after a single oral dose of Cd (20 mg Cd/kg b.w.). In the subacute experiment, mice were given 10 mg Cd/kg b.w. orally every day for 14 days; Fe and MDA contents were determined in liver after 1 and 2 weeks. Acute Cd intoxication induced a significantly increased hepatic Fe content after 4 and 6h, and a statistically significant increase in MDA 6, 12 and 24h after Cd administration, although a significantly decreased MDA level was observed after 48 h. The results suggest development of early oxidative stress in livers of mice after acute intoxication with Cd. The decreased MDA observed after 48 h occurred presumably due to the adaptive response of the organism. Subacute Cd intoxication induced a significant decrease of hepatic Fe and MDA levels at both investigated time intervals compared with control. These results indicate a positive correlation between hepatic Fe and MDA content and suggest that prolonged Cd intoxication decreases hepatic LPO indirectly, by reducing the Fe content of mouse liver.
Djukić-Cosić
Danijela
D
Institute of Toxicology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia and Montenegro. dakitox@pharmacy.bg.ac.yu
Curcić Jovanović
Marijana
M
Plamenac Bulat
Zorica
Z
Ninković
Milica
M
Malicević
Zivorad
Z
Matović
Vesna
V
D000818:Animals; D002104:Cadmium / Q000008:administration & dosage / Q000633:toxicity*; D007501:Iron / Q000378:metabolism*; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D023382:Toxicity Tests, Acute
D002104:Cadmium; D008315:Malondialdehyde; D007501:Iron