Reduced spontaneous relaxation in immature guinea pig airway smooth muscle is associated with increased prostanoid release.
Airway smooth muscle (ASM) from infant guinea pigs has less spontaneous relaxation during stimulation than ASM from adults. Inhibition of cyclooxygenase (COX), which catalyzes the production of prostanoids, increases this relaxation in infant ASM and abolishes age differences, thus suggesting that prostanoids reduce relaxation in infant ASM. In this study, we investigated whether leukotrienes are also involved in reducing spontaneous relaxation; whether the two COX isoforms, COX-1 and COX-2, differentially regulate spontaneous relaxation; and whether prostanoid release is developmentally regulated in guinea pig ASM. In different age groups, we measured relaxation during and after electrical stimulation in tracheal strips as well as prostanoid release from tracheal segments. Relaxation was studied in the absence and in the presence of a lipoxygenase inhibitor, a cysteinyl leukotriene receptor-1 antagonist, a COX-1 inhibitor, or a COX-2 inhibitor. We found that inhibition of lipoxygenase or cysteinyl leukotriene receptor-1 antagonism did not increase spontaneous relaxation at any age, thus excluding a role for leukotrienes in this phenomenon. Inhibition of COX-2, but not COX-1, promoted spontaneous relaxation. The basal release of prostanoids was more abundant in tissue from infant animals and decreased significantly with age. Thromboxane B2 was the most abundant metabolite released at all ages. Electrical stimulation and epithelium removal did not affect the age difference in prostanoid release. We conclude that increased basal prostanoid release contributes to the reduced spontaneous relaxation in immature guinea pig ASM compared with older animals. By regulating ASM relaxation, prostanoids may play a role in the airway hyperresponsiveness at a young age.
Wang
Lu
L
Division of Pediatric Pulmonary Medicine, Department of Pediatrics, Duke University Medical Center, Durham, NC, USA.
Pozzato
Valeria
V
Turato
Graziella
G
Madamanchi
Aasakiran
A
Murphy
Thomas M
TM
Chitano
Pasquale
P
D000367:Age Factors; D000818:Animals; D001986:Bronchial Spasm / Q000503:physiopathology*; D016084:Bronchoconstriction / Q000187:drug effects / Q000502:physiology*; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D007555:Isoxazoles / Q000494:pharmacology; D015289:Leukotrienes / Q000378:metabolism; D009130:Muscle, Smooth / Q000502:physiology*; D011453:Prostaglandins / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology; D013450:Sulfones / Q000494:pharmacology; D014132:Trachea / Q000502:physiology*
C501048:3-(4-methylsulfonylphenyl)-4-phenyl-5-trifluoromethylisoxazole; D016861:Cyclooxygenase Inhibitors; D007555:Isoxazoles; D015289:Leukotrienes; D011453:Prostaglandins; D011720:Pyrazoles; C115461:SC 560; D013450:Sulfones; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2