Paenibacillus woosongensis sp. nov., a xylanolytic bacterium isolated from forest soil.
A novel xylan-degrading bacterium, YB-45(T), was isolated from forest soil. The organism is a facultatively anaerobic, Gram-variable, motile, endospore-forming, rod-shaped bacterium. It grew optimally at 37 degrees C and pH 7.5 in the presence of 3 % (w/v) NaCl. The predominant cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and C(16 : 0). The DNA G+C content was 51.7 mol% and the predominant menaquinone was MK-7. Growth was observed with many carbohydrates, including xylan, as sole carbon sources. Strain YB-45(T) produces a wide variety of hydrolytic enzymes, such as xylanase, cellulase, amylase, beta-mannanase, beta-mannosidase, beta-xylosidase, alpha-galactosidase, beta-galactosidase and beta-glucosidase. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YB-45(T) belongs to the genus Paenibacillus, sharing sequence similarity that was <96 %. It was related most closely to Paenibacillus jamilae DSM 13815(T), with 95.7 % sequence similarity. On the basis of morphological, chemotaxonomic, physiological and phylogenetic properties, strain YB-45(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus woosongensis sp. nov. is proposed. The type strain is YB-45(T) (=KCTC 3953(T)=DSM 16971(T)).
Lee
Jae-Chan
JC
Korea Research Institute of Bioscience and Biotechnology, 52 Oeun-dong, Yusong-gu, Daejeon 305-333, Republic of Korea.
Yoon
Ki-Hong
KH
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014197:Trees; D014990:Xylans / Q000378:metabolism*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014990:Xylans