Distinct immunohistochemically defined areas in the medial amygdala in the developing and adult mouse.
The medial amygdala has been considered a subpallial structure, but various studies have shown that is a somewhat complex structure expressing both pallial and subpallial gene markers. In this regard, we analyzed the immunohistochemical expression of the neurotransmitter GABA, the vesicular glutamate transporter type 2 (VGLUT2), the neuronal nitric oxide synthase (nNOS), and the calcium-binding proteins calbindin-D28k (CB) and calretinin (CR) in the developing and adult mouse medial amygdala. From intermediate embryonic stages on, neurochemical data show a distinctive superficial region forming a band all along the medial amygdalar surface. This superficial band displays a strong VGLUT2-immunoreactive neuropil and numerous CR-immunoreactive fibers, as well as some nNOS-, CR- or CB-positive cells. In contrast, the superficial region of the posterior medial amygdala appears to be non-GABA immunoreactive. This band in the posterior medial amygdala matches a Tbr1-expressing territory. Our results also show differences between dorsal and ventral parts of the postnatal and adult posterior medial amygdala. Especially, a compact cell aggregate of nNOS immunoreactive cells was found in the ventral portion of the medial amygdala, whereas the dorsal part is occupied by scattered weakly stained cells. Comparison of our results with gene expression patterns and fiber-tracing studies, let us to propose that the superficial band is a pallial derivative, whereas the dorsal and ventral nuclei of the posterior medial amygdala receive each neurons from different subpallial compartments, and the latter one a subset of excitatory, pallial projection neurons.
Guirado
Salvador
S
Department of Cell Biology, Genetics and Physiology, Faculty of Biology, University of Málaga, Campus de Teatinos, 29071 Malaga, Spain. guirado@uma.es
Real
M Angeles
MA
Dávila
José Carlos
JC
D000679:Amygdala / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D004622:Embryo, Mammalian; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
C576499:Calb1 protein, mouse; C576511:Calb2 protein, mouse; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D050599:Vesicular Glutamate Transport Protein 2; D005680:gamma-Aminobutyric Acid; D052248:Nitric Oxide Synthase Type I