title: Cellular prion protein in mammary gland and milk fractions of domestic ruminants.
abstract: The present study shows that PrP(c) is expressed in the mammary gland and milk fractions of domestic ruminants in a species-specific manner. By applying immunohistochemistry, Western blot and ELISA, clear expression differences between bovine, ovine and caprine mammary gland, skimmed milk, acid whey and cream could be demonstrated, the highest relative PrP(c) levels being associated with the cream fraction. In the bovine gland PrP(c) was preferentially detectable at the basolateral surface of mammary gland epithelial cells, whereas in ovine and caprine samples the prion protein was more homogeneously distributed. Moreover, in ovine and caprine bovine mammary gland epithelial cells, apocrine secretory vesicles were strongly stained. Ovine and caprine milk proved to contain PrP(c) in all fractions with an additional truncated form at 12kDa in Western blot. This truncated isoform is the predominate one in caprine acid whey. These results support the hypothesis that the apocrine secretion mode of milk fat globules is a major way of PrP(c) transport into the milk.
authors.affiliation: Chair for Hygiene and Technology of Milk, Ludwig Maximilians University, Schoenleutnerstrasse 8, 85764 Oberschleissheim, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Didier
authors.forename: R
authors.initials: R
authors.lastname: Gebert
authors.forename: R
authors.initials: R
authors.lastname: Dietrich
authors.forename: M
authors.initials: M
authors.lastname: Schweiger
authors.forename: M
authors.initials: M
authors.lastname: Gareis
authors.forename: E
authors.initials: E
authors.lastname: Märtlbauer
authors.forename: W M
authors.initials: WM
authors.lastname: Amselgruber
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006041:Goats; D007150:Immunohistochemistry; D007774:Lactation; D008321:Mammary Glands, Animal / Q000737:chemistry* / Q000378:metabolism*; D008892:Milk / Q000737:chemistry*; D018621:PrPC Proteins / Q000032:analysis* / Q000378:metabolism*; D034561:Sheep, Domestic
chemical_list: D018621:PrPC Proteins