title: High throughput screening of gene functions in mammalian cells using reversely transfected cell arrays: review and protocol.
abstract: Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or--by using the recently developed RNA interference (RNAi) techniques--knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.
authors.affiliation: Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Schwabachanlage 10, D-91054 Erlangen, Germany. michael.stuerzl@uk-erlangen.de
authors.forename: Michael
authors.initials: M
authors.lastname: Stürzl
authors.forename: Andreas
authors.initials: A
authors.lastname: Konrad
authors.forename: Gaby
authors.initials: G
authors.lastname: Sander
authors.forename: Effi
authors.initials: E
authors.lastname: Wies
authors.forename: Frank
authors.initials: F
authors.lastname: Neipel
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Naschberger
authors.forename: Simone
authors.initials: S
authors.lastname: Reipschläger
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gonin-Laurent
authors.forename: Raymund E
authors.initials: RE
authors.lastname: Horch
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kneser
authors.forename: Werner
authors.initials: W
authors.lastname: Hohenberger
authors.forename: Holger
authors.initials: H
authors.lastname: Erfle
authors.forename: Mathias
authors.initials: M
authors.lastname: Thurau
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D020868:Gene Silencing; D005796:Genes / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D048348:Reverse Transcription / Q000235:genetics*; D046888:Tissue Array Analysis / Q000379:methods*; D014162:Transfection / Q000379:methods*