Maximal inflammatory response benefits syngeneic skin graft acceptance.
OBJECTIVE
We investigated the influence of acute inflammation in skin isograft acceptance.


METHODS
Two mouse lines selected for maximal (AIRMAX) or minimal inflammatory response (AIRMIN) were transplanted with syngeneic skin. Cellular infiltrates and cytokine production were measured 1, 3, 7 or 14 days post-transplantation. The percentage of CD4+CD25+Foxp3+ cells in the lymph nodes was also evaluated.


RESULTS
Grafts were totally accepted in 100% of AIRMAX and in 26% of AIRMIN mice. In the latter, partial acceptance was observed in 74% of the animals. Emigrated cells were basically PMN and were enhanced in AIRMAX transplants. IL-10 production by graft infiltrating cells showed no interline differences. IFN-gamma was increased in AIRMIN grafts at day 14 and lower percentages of CD4+CD25+Foxp3+ cells in the lymph nodes were observed in these mice.


CONCLUSIONS
Our data suggest that differences in graft acceptance might be due to a lack of appropriate regulation of the inflammatory response in AIRMIN mice compromising the self/non-self recognition.
Larocca
R
R
Laboratory of Clinical Immunology, Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Marguti
I
I
Cabrera
W
W
Ribeiro
O Garcia
OG
Rizzo
L V
LV
de Moraes
L Vieira
LV
D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006085:Graft Survival / Q000502:physiology*; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D023421:Models, Animal; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology*; D016038:Skin Transplantation / Q000473:pathology / Q000502:physiology*; D014185:Transplantation, Isogeneic / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015704:CD4 Antigens; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma