title: [Optimization of water extraction condition of LiYan granule by Doehlert design].
abstract: OBJECTIVE
  To study optimal water extraction condition of LiYan granule.
  
  
  METHODS
  HPLC methods were developed for the determination of irisfloremin in LiYan granule. The contents of irisfloremin and extraction rate were selected as assessment indices. Three factors were studied by Doehlert design, including water-adding amount, decoction time and times of decoction.
  
  
  RESULTS
  The optimal extracting condition was water consumed as 8 times of curd herb, and 3 times for 2 hours each time.
  
  
  CONCLUSIONS
  The water extraction process is feasible, and the experiment result can provide basic and instruction data for the ascertainment of water extraction process of LiYan granule.
authors.affiliation: Nanjing Medicial University, Nanjing 210029, China. lyptjfll@163.com
authors.forename: Jian-fei
authors.initials: JF
authors.lastname: Tao
authors.forename: Li-na
authors.initials: LN
authors.lastname: Chen
authors.forename: Shu-hu
authors.initials: SH
authors.lastname: Du
mesh_terms: D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D012107:Research Design; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014867:Water
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012997:Solvents; C530396:liyan; D014867:Water