Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography.
A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmol l(-1) borate, 20 mmol l(-1) phosphate, pH 8.4, containing 20 mmol l(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25 degrees C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients > or =0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.
Injac
Rade
R
Faculty of Pharmacy, Institute of Pharmaceutical Biology, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia. injacrade@gmail.com
Mlinaric
Ales
A
Djorjevic-Milic
Vukosava
V
Karljikovic-Rajic
Katarina
K
Strukelj
Borut
B
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005503:Food Additives; D010118:Oxytetracycline / Q000032:analysis*; D010456:Peptides, Cyclic / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013409:Sulfacetamide / Q000032:analysis*; D013552:Swine
D000900:Anti-Bacterial Agents; D005503:Food Additives; D010456:Peptides, Cyclic; D013409:Sulfacetamide; D010118:Oxytetracycline