Inducing chondrogenic differentiation in injectable hydrogels embedded with rabbit chondrocytes and growth factor for neocartilage formation.
A thermoreversible hydrogel of poly(NiPAAm-co-AAc) was used as an injectable cell and growth factor delivery carrier for cartilage tissue engineering. Rabbit chondrocytes were embedded in composite hydrogels coencapsulated with transforming growth factor beta3 (TGFbeta3). Hydrogel constructs consisting of embedded cells encapsulated by the thermoreversible hydrogel served as controls to assess the effects of TGFbeta3 on chondrogenic differentiation. The hydrogel constructs were injected subcutaneously into nude mice and then monitored for up to 8 weeks after injection. After 8 weeks of implantation, the engineered cartilage acquired normal histological and biochemical properties. These results highlight the potential of growth factor in a hydrogel embedded with chondrocytes as a candidate material for neocartilage formation.
Yun
Kyusik
K
Gachon BioNano Research Institute, Kyungwon University, San 65, Bokjeong-Dong, Sujeong-gu, Seongnam-Si, Gyeonggi-Do 461-701, Korea.
Moon
Hyun Tae
HT
D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D020219:Chondrogenesis / Q000187:drug effects / Q000502:physiology*; D004337:Drug Carriers / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D053782:Transforming Growth Factor beta3 / Q000008:administration & dosage* / Q000737:chemistry
D004337:Drug Carriers; D020100:Hydrogels; D053782:Transforming Growth Factor beta3