Comparison of two basophil activation markers CD63 and CD203c in the diagnosis of amoxicillin allergy.
BACKGROUND
To confirm allergy to beta-lactam (BL), a basophil activation test in flow cytometry based on CD63 up-regulation was described. CD203c is a more recent basophil activation marker and up to day there is no consensus about which marker is the more sensitive one. CD203c has not yet been evaluated in the diagnosis of BL allergy.


OBJECTIVE
The aim of the study was to compare the reliability of CD203c to CD63 for the diagnosis of amoxicillin (AX) allergy, which is nowadays the most frequent BL allergy.


METHODS
Twenty-seven patients with an immediate positive skin test (ST) to AX, 20 had had anaphylaxis with AX and 7 had urticaria and/or angioedema, were compared with 14 controls with no allergy to BL and to six patients with delayed positive ST to AX.


RESULTS
In the anaphylaxis group, AX induced up-regulation of CD203c in the basophils of 12 patients out of 20 (60%) and of CD63 in four patients (20%) (P<0.02). Two patients out of seven with urticaria or angioedema had a positive result with CD203c and CD63. In patients who had anaphylaxis, ampicillin (AMP) induced CD203c up-regulation in eight out of 12 (67%) patients tested, and CD63 up-regulation in 4 out of 12 (33%) (all patients who had anaphylaxis could not be tested with AMP). False-positive results were observed with CD203c as well as CD63, and for 10 patients indeed this was confirmed by a negative drug provocation test. The origin of conflicting results between CD63 and CD203c might be at least the targeting of basophils based on anti-IgE labelling. Among IgE(+) gated cells, by means of CD33, a marker of monocytes, a contamination up to 50% by monocytes was detected. In contrast to CD63, CD203c is an activation marker specific of basophils with a basal low-level expression in resting basophils. Thus, IgE and CD203c double targeting of basophils avoids the contamination by monocytes.


CONCLUSIONS
CD203c seems to be a more sensitive activation marker of basophils than CD63 for the diagnosis of amoxicillin allergy.
Abuaf
N
N
Department of Hematology and Immunology, Hôpital Tenon and Université e Pierre et Marie Curie, Paris, France. nissen.abuaf@tnn.aphp.fr
Rostane
H
H
Rajoely
B
B
Gaouar
H
H
Autegarden
J E
JE
Leynadier
F
F
Girot
R
R
D000328:Adult; D000368:Aged; D000658:Amoxicillin / Q000009:adverse effects*; D000707:Anaphylaxis / Q000139:chemically induced / Q000175:diagnosis / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism*; D001491:Basophils / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D005189:False Positive Reactions; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000175:diagnosis / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000175:diagnosis* / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism; D007159:Immunologic Tests / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D012882:Skin Tests; D060149:Tetraspanin 30; D015854:Up-Regulation; D014581:Urticaria / Q000139:chemically induced / Q000175:diagnosis / Q000276:immunology
D015703:Antigens, CD; D015415:Biomarkers; C554586:CD63 protein, human; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D007073:Immunoglobulin E; D000658:Amoxicillin