Long-term bone marrow cultures (LTBMC) from essential thrombocythemia (ET) patients with or without JAK2617V>F mutation.
Approximately half of essential thrombocythemia (ET) patients and almost all with polycythemia vera (PV) bear the activating JAK2617V>F point mutation, which arises at the multipotent haemopoietic progenitor cell level. Although ET is mainly characterized by megacaryocyte proliferation, the cases that are positive for the JAK2617V>F mutation also show increased bone marrow cellularity and higher erythrocyte and granulocyte counts. After establishing short- and long-term bone marrow cultures we found that the frequency of committed haemopoietic progenitors in the bone marrow, was not increased in JAK2617V>F positive ET compared to the negative ones, whereas in long-term cultures (LTBMC) JAK2617V>F positive ET display a growth pattern more similar to that observed in LTBMC produced by PV marrow cells. Our data support the notion that JAK2617V>F positive ET and PV represents a continuum spectrum of alterations within the same disease.
Spanoudakis
E
E
Department of Haematology, Democritus University of Thrace, Alexandroupolis, Greece.
Margaritis
D
D
Kotsianidis
I
I
Georgiou
G
G
Tripsianis
G
G
Anastasiadis
A
A
Karakolios
A
A
Pantelidou
D
D
Panayiotidis
P
P
Bourikas
G
G
Tsatalas
C
C
D019943:Amino Acid Substitution; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000235:genetics*; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics*; D017354:Point Mutation; D011087:Polycythemia Vera / Q000473:pathology; D013920:Thrombocythemia, Essential / Q000235:genetics* / Q000473:pathology
C507924:JAK2 protein, human; D053614:Janus Kinase 2