PEDF from mouse mesenchymal stem cell secretome attracts fibroblasts.
Conditioned medium (secretome) derived from an enriched stem cell culture stimulates chemotaxis of human fibroblasts. These cells are classified as multipotent murine mesenchymal stromal cells (mMSC) by immunochemical analysis of marker proteins. Proteomic analysis of mMSC secretome identifies nineteen secreted proteins, including extracellular matrix structural proteins, collagen processing enzymes, pigment epithelium-derived factor (PEDF) and cystatin C. Immunodepletion and reconstitution experiments show that PEDF is the predominant fibroblast chemoattractant in the conditioned medium, and immunofluorescence microscopy shows strong staining for PEDF in the cytoplasm, at the cell surface, and in intercellular space between mMSCs. This stimulatory effect of PEDF on fibroblast chemotaxis is in contrast to the PEDF-mediated inhibition of endothelial cell migration, reported previously. These differential functional effects of PEDF toward fibroblasts and endothelial cells may serve to program an ordered temporal sequence of scaffold building followed by angiogenesis during wound healing.
Sarojini
Harshini
H
Gheens Center on Aging, University of Louisville School of Medicine, Louisville, Kentucky 40202, USA.
Estrada
Rosendo
R
Lu
Hongwei
H
Dekova
Silvia
S
Lee
Menq-Jer
MJ
Gray
Robert D
RD
Wang
Eugenia
E
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002633:Chemotaxis; D017077:Culture Media, Conditioned; D005136:Eye Proteins / Q000737:chemistry / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D013058:Mass Spectrometry; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D015203:Reproducibility of Results; D015843:Serpins / Q000737:chemistry / Q000378:metabolism*; D000099174:Pigment Epithelium-Derived Factor
D017077:Culture Media, Conditioned; D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D000099174:Pigment Epithelium-Derived Factor