Radioautographic study of the seasonal distribution of leukocytes in turtles Phrynops hilarii (Chelonia Chelidae).
The aim of this study was to present a morphological description of the leukocytes of Phrynops hilarii turtles according to the seasonal distribution of these cells and to show their replacement in the blood circulation using a radioautographic method. Five animals of both sexes weighing 600-1200 g were used. The animal's blood was aspirated, smeared on glass slides, and stained with the Romanowsky stain, and 500 cells of each animal were counted during each season. The results obtained were analyzed statistically by analysis of variance followed by the Bonferroni test (NCSS), with the level of significance set at p<0.05. The radioautographic analysis of turtle blood exposed to 1000 microCi of (3)H-thymidine and developed after 30 days showed a large number of silver grains incorporated into the cells, except for basophils, with cell renewal occurring every seven days. Quantitative data demonstrated a seasonal influence on the distribution of some leukocyte types, with the following "p" values: heterophils (p=0.0007), basophils (p=0.0002), monocytes (p=0.0016), eosinophils (p=0.0073). However, using this statistical method, it was not possible to detect a significant difference related to seasonal influence on lymphocytes (p=0.16295) or thrombocytes (p=0.1046). Using this experimental animal model, a seasonal influence on the distribution of some leukocyte types was observed, and the radioautographic method revealed a cell renewal system occurring every seven days, except for basophils.
Pitol
Dimitrius Leonardo
DL
Biosciences Institute-Molecular and Cellular Biology (UNESP), Rio Claro, São Paulo, Brazil. dimipitol@yahoo.com.br
Issa
João Paulo Mardegan
JP
Caetano
Flávio Henrique
FH
Lunardi
Laurelúcia Orive
LO
D000818:Animals; D001345:Autoradiography; D007962:Leukocytes / Q000166:cytology*; D012621:Seasons; D014426:Turtles / Q000097:blood*