title: Infection trials in pigs with a human isolate of Brucella ( isolate 02/611 'marine mammal type' ).
abstract: OBJECTIVE
  To determine if pigs could support infection of a human Brucella isolate (Brucella 02/611) from New Zealand, and to study seroconversion to this isolate using a competitive ELISA.
  
  
  METHODS
  Ten weaner piglets were challenged with 4.8 x 10(8) cfu of organisms by the oral and ocular routes. Culture was attempted on blood samples taken prior to challenge, and 4, 7, 9, 11, 14, 21 and 28 days post-challenge, and on tissue samples taken at the termination of the trial, 1 month after challenge. Sera were analysed for antibody using an ELISA. For reference comparison, similar trials were conducted in two pigs using an isolate of Brucella suis biovar 1, and two pigs using an isolate of B. suis biovar 3.
  
  
  RESULTS
  Brucella 02/611 organisms were re-isolated from one lymph node each from three pigs; all other samples were negative. Low and transient antibody titres were detected using a competitive ELISA in three pigs, two of which were culture negative. Organisms of B. suis reference strains were re-isolated from multiple samples from each of the four animals.
  
  
  CONCLUSIONS
  Brucella 02/611 does not seem to replicate readily in pigs. It is unlikely that pigs were the original maintenance hosts for Brucella 02/611.
authors.affiliation: CSIRO Australian Animal Health Laboratory, Private Bag 24, Geelong, Victoria 3220, Australia. john.bingham@csiro.au
authors.forename: J
authors.initials: J
authors.lastname: Bingham
authors.forename: T K
authors.initials: TK
authors.lastname: Taylor
authors.forename: J E
authors.initials: JE
authors.lastname: Swingler
authors.forename: G
authors.initials: G
authors.lastname: Meehan
authors.forename: D J
authors.initials: DJ
authors.lastname: Middleton
authors.forename: G F
authors.initials: GF
authors.lastname: Mackereth
authors.forename: J S
authors.initials: JS
authors.lastname: O'Keefe
authors.forename: P W
authors.initials: PW
authors.lastname: Daniels
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002002:Brucella / Q000276:immunology / Q000472:pathogenicity*; D002006:Brucellosis / Q000382:microbiology / Q000662:veterinary*; D015169:Colony Count, Microbial / Q000662:veterinary; D004197:Disease Reservoirs / Q000662:veterinary; D004198:Disease Susceptibility / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014886:Weaning
chemical_list: D000907:Antibodies, Bacterial