AFM study on the electric-field effects on supported bilayer lipid membranes.
Electric-field induced changes in structure and conductivity of supported bilayer lipid membranes (SLM) have been studied at submicroscopic resolution using atomic force microscopy and electrochemical impedance spectroscopy. The SLMs are formed on gold surfaces modified with mixed self-assembled monolayers of a cholesterol-tether and 6-mercaptohexanol. At applied potentials of < or =-0.25 V versus standard hydrogen electrode, the conductance of the SLM increases and membrane areas of <150 nm in size are found to elevate from the surface up to 15 nm in height. To estimate the electric field experienced by the lipid membrane, electrowetting has been used to determine the point of zero charge of a 6-mercaptohexanol-modified surface (0.19 +/- 0.13 V versus standard hydrogen electrode). The effects of electric fields on the structure and conductance of supported membranes are discussed.
Jeuken
Lars J C
LJ
Institute of Membrane and Systems Biology, University of Leeds, Leeds, United Kingdom. l.j.c.jeuken@leeds.ac.uk
D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields; D008051:Lipid Bilayers / Q000737:chemistry* / Q000528:radiation effects*; D008560:Membrane Fluidity / Q000528:radiation effects*; D018625:Microscopy, Atomic Force / Q000379:methods*
D008051:Lipid Bilayers