title: Effect of adding extracted hesperetin, naringenin and pectin on egg cholesterol, serum traits and antioxidant activity in laying hens.
abstract: In this study three feed additives (hesperetin, naringenin and pectin) for laying hens were investigated on their influence on the egg yolk cholesterol, serum traits and antioxidant activities in hens. Additives were extracted from citrus and grapefruit peels and contained 31.5% crude hesperetin, 39% crude naringenin and 60% galacturonic acid (pectin). Eighty 30-week-old Leghorn laying hens were randomly assigned to four groups and received, for two months, a control diet or diets with 0.05% hesperetin, 0.05% naringenin or 0.5% pectin. All additives reduced the egg yolk cholesterol level significantly. Feeding diets with added flavonoids (hesperetin and naringenin) increased the yolk weight and the ratio of yolk weight/egg weight and the blood serum superoxide dismutase (SOD) activity was elevated. Total antioxidation capacity, the level of thiobarbituric acid-reactive substances and superoxide scavenging capacity in the naringenin group were greater than in the control group. Supplemented flavonoids reduced the serum cholesterol level significantly, while serum triglyceride concentration in the naringenin and pectin groups was reduced. Addition of flavonoids resulted in an enhanced cholesterol level in excreta. The results of this study indicated that intake of hesperetin, naringenin and pectin extracted from citrus and grapefruit peel in laying hens diet, may exhibit positive effects.
authors.affiliation: Department of Animal Science, National Chiayi University, Chiayi, Taiwan, ROC. tflien@mail.ncyu.edu.tw
authors.forename: Tu Fa
authors.initials: TF
authors.lastname: Lien
authors.forename: Hui Shuang
authors.initials: HS
authors.lastname: Yeh
authors.forename: Wu Tien
authors.initials: WT
authors.lastname: Su
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002645:Chickens / Q000097:blood / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D004530:Egg Yolk / Q000737:chemistry; D004531:Eggs / Q000032:analysis*; D005260:Female; D044950:Flavanones / Q000494:pharmacology*; D016166:Free Radical Scavengers; D006569:Hesperidin / Q000494:pharmacology*; D050356:Lipid Metabolism; D010368:Pectins / Q000494:pharmacology*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D044950:Flavanones; D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D010368:Pectins; D002784:Cholesterol; D006569:Hesperidin; D013482:Superoxide Dismutase; C005273:naringenin; C013015:hesperetin