title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of grass carp beta2-microglobulin.
abstract: Beta(2)-microglobulin (beta(2)m) is an essential subunit of MHC I molecules; it stabilizes the structure of MHC I and plays a pivotal role in coreceptor recognition. To date, structures of beta(2)m have been solved for three different mammals: human, mouse and cattle. In order to illuminate the molecular evolutionary origin of beta2m, an understanding of its structure in lower vertebrates becomes important. Here, grass carp (Ctenopharyngodon idellus) beta(2)m (Ctid-beta(2)m) was expressed, purified and crystallized. Diffraction data were collected to a resolution of 2.5 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.72, b = 40.65, c = 71.12 A. The Matthews coefficient and the solvent content were calculated to be 2.56 A Da(-1) and 52.07%, respectively, for one molecule per asymmetric unit. The structure has been solved by molecular replacement using monomeric human beta(2)m as a model.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, People's Republic of China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Chen
authors.forename: Fuliang
authors.initials: F
authors.lastname: Chu
authors.forename: Hao
authors.initials: H
authors.lastname: Peng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Fan
authors.initials: F
authors.lastname: Jiang
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D002850:Chromatography, Gel; D003460:Crystallization; D015870:Gene Expression; D014961:X-Ray Diffraction; D001613:beta 2-Microglobulin / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001613:beta 2-Microglobulin