title: Preparation and evaluation of solid-phase microextraction fiber based on molecularly imprinted polymers for trace analysis of tetracyclines in complicated samples.
abstract: Molecularly imprinted polymer (MIP) is widely used in many fields because of its characteristics of high selectivity, chemical stability and easy preparation. To enhance the selectivity and applicability of solid-phase microextraction (SPME), a novel MIP-coated SPME fiber was firstly prepared by multiple co-polymerization method with tetracycline as template. It could be coupled directly to high-performance liquid chromatography (HPLC) and used for trace analysis of tetracyclines (TCs) in complicated samples. The characteristics and application of the fibers were investigated. The electron microscope provided a crosslinked and porous surface, and the average thickness of the MIP coating was 19.5 microm. Compared with the non-imprinted polymer (NIP) coated fibers, the special selectivity to tetracycline and structure-similar oxytetracycline, doxycycline, chlortetracycline were discovered with the MIP-coated fibers. The adsorption and desorption of TCs with the MIP-coated fiber could be achieved quickly. A method for the fluorimetric determination of four TCs by the MIP-coated SPME coupled with HPLC was developed. The optimized extraction conditions such as extraction solvent, desorption solvent, and stirring speed were studied. Linear ranges for the four TCs were 5.00-200 microg/L and detection limits were within the range of 1.0-2.3 microg/L. The method was applied to simultaneous multi-residue analysis of four TCs in the spiked chicken feed, chicken muscle, and milk samples with the satisfactory recoveries.
authors.affiliation: School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Hu
authors.forename: Jialiang
authors.initials: J
authors.lastname: Pan
authors.forename: Yuling
authors.initials: Y
authors.lastname: Hu
authors.forename: Yin
authors.initials: Y
authors.lastname: Huo
authors.forename: Gongke
authors.initials: G
authors.lastname: Li
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D012015:Reference Standards; D012822:Silicon Dioxide / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*; D012996:Solutions; D013754:Tetracyclines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013997:Time Factors
chemical_list: D011108:Polymers; D012996:Solutions; D013754:Tetracyclines; D012822:Silicon Dioxide