title: SLP-2 interacts with prohibitins in the mitochondrial inner membrane and contributes to their stability.
abstract: Stomatin is a member of a large family of proteins including prohibitins, HflK/C, flotillins, mechanoreceptors and plant defense proteins, that are thought to play a role in protein turnover. Using different proteomic approaches, we and others have identified SLP-2, a member of the stomatin gene family, as a component of the mitochondria. In this study, we show that SLP-2 is strongly associated with the mitochondrial inner membrane and that it interacts with prohibitins. Depleting HeLa cells of SLP-2 lead to increased proteolysis of prohibitins and of subunits of the respiratory chain complexes I and IV. Further supporting the role of SLP-2 in regulating the stability of specific mitochondrial proteins, we found that SLP-2 is up-regulated under conditions of mitochondrial stress leading to increased protein turnover. These data indicate that SLP-2 plays a role in regulating the stability of mitochondrial proteins including prohibitins and subunits of respiratory chain complexes.
authors.affiliation: Department of Cell Biology, University of Geneva, Geneva, Switzerland.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Da Cruz
authors.forename: Philippe A
authors.initials: PA
authors.lastname: Parone
authors.forename: Philippe
authors.initials: P
authors.lastname: Gonzalo
authors.forename: Willy V
authors.initials: WV
authors.lastname: Bienvenut
authors.forename: Daniel
authors.initials: D
authors.lastname: Tondera
authors.forename: Alexis
authors.initials: A
authors.lastname: Jourdain
authors.forename: Manfredo
authors.initials: M
authors.lastname: Quadroni
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Martinou
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D042967:Electron Transport Complex I / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D000091402:Prohibitins; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000091402:Prohibitins; D012097:Repressor Proteins; C406287:STOML2 protein, human; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I