title: Changes in peripheral lymphocyte subsets in the type 1 diabetic dogs treated with insulin injections.
abstract: Plasma metabolites and peripheral lymphocyte subsets were measured in ten diabetic and ten control dogs to investigate their significances as indicators to evaluate immune states in the diabetic dogs. Diabetic dogs were treated with insulin injections, however their plasma glucose and fructosamine concentrations were significantly higher than those of the controls. There were no significant differences in counts of total white blood cells (WBC) and lymphocyte CD8(+) cells (cytotoxic T cells) between the control and the diabetic dogs. In the diabetic dogs, the counts of CD3(+) (T cells), CD4(+) (Helper T cells) and CD21(+) (B cells) cells and the peripheral lymphocytes CD4/CD8 ratio were significantly lower than those in the control dogs. We confirmed abnormality of lymphocyte subsets in insulin treated diabetic dogs and it may relate to depression of immunocompetence and high susceptibility to common infectious diseases.
authors.affiliation: Department of Veterinary Science, School of Veterinary Medicine, Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan. D0608@nvlu.ac.jp
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mori
authors.forename: Fumi
authors.initials: F
authors.lastname: Sagara
authors.forename: Sumire
authors.initials: S
authors.lastname: Shimizu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Mizutani
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sako
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hirose
authors.forename: Itaru
authors.initials: I
authors.lastname: Yoshimura
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Uematsu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
mesh_terms: D000818:Animals; D001786:Blood Glucose; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D004283:Dog Diseases / Q000188:drug therapy* / Q000276:immunology; D004285:Dogs; D005260:Female; D019270:Fructosamine / Q000097:blood; D007328:Insulin / Q000494:pharmacology / Q000627:therapeutic use*; D016131:Lymphocyte Subsets / Q000187:drug effects*; D008297:Male
chemical_list: D001786:Blood Glucose; D007328:Insulin; D019270:Fructosamine