A decade of yeast surface display technology: where are we now?
Yeast surface display has become an increasingly popular tool for protein engineering and library screening applications. Recent advances have greatly expanded the capability of yeast surface display, and are highlighted by cell-based selections, epitope mapping, cDNA library screening, and cell adhesion engineering. In this review, we discuss the state-of-the-art yeast display methodologies and the rapidly expanding set of applications afforded by this technology.
Pepper
Lauren R
LR
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
Cho
Yong Ku
YK
Boder
Eric T
ET
Shusta
Eric V
EV
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D018604:Epitope Mapping; D006801:Humans; D015202:Protein Engineering; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary