Enzymatic preparation of cefaclor with immobilized penicillin acylase.
Enzymatic syntheses of cefaclor by immobilized penicillin acylase under kinetic control were carried out. According to the initial reaction rate ratio of synthesis to hydrolysis (Vs/Vh), penicillin acylase from Alcaligenes faecalis was chosen as the suitable catalyst for the synthesis of cefaclor. The reaction conditions, such as temperature, pH, and substrate concentration were investigated based on their Vs/Vh values. In the process of preparing cefaclor, in situ product removal (ISPR) and acyl donor feeding were used to achieve high yield. At the optimal conditions, the yield of cefaclor was 90%. In addition, the product were separated and purified, the total yield of cefaclor was 61%.
Zhang
Ye-Wang
YW
Department of Pharmaceutical Engineering, School of Pharmaceutics, Jiangsu University, Zhenjiang, PR China.
Wei
Dong-Zhi
DZ
D000421:Alcaligenes / Q000201:enzymology*; D002384:Catalysis; D002511:Cephalosporins / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D010406:Penicillins / Q000737:chemistry*; D010880:Piperidines / Q000138:chemical synthesis*
D002511:Cephalosporins; D004800:Enzymes, Immobilized; D010406:Penicillins; D010880:Piperidines; C014320:7-aminodesacetoxycephalosporanic acid; C004858:diphenidol