Facile synthesis of methyl alpha- and beta-D-[6-(3)H]galactofuranosides from D-galacturonic acid. Substrates for the detection of galactofuranosidases.
Galactofuranose metabolism is a good target for the development of novel chemotherapeutic agents for the treatment of some microbial infections. A simple procedure for the synthesis of methyl (methyl alpha,beta-D-galactopyranosid)uronate followed by NaB(3)H(4) reduction gave a straightforward access to radiolabeled substrates for galactofuranosidases.
Bordoni
Andrea
A
CIHIDECAR (CONICET), Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina.
Lima
Carlos
C
Mariño
Karina
K
de Lederkremer
Rosa M
RM
Marino
Carla
C
D005663:Furans / Q000138:chemical synthesis; D006026:Glycoside Hydrolases / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods; D008756:Methylgalactosides / Q000138:chemical synthesis*; D013237:Stereoisomerism; D014316:Tritium
D005663:Furans; D006603:Hexuronic Acids; D008756:Methylgalactosides; D014316:Tritium; C007819:galacturonic acid; D006026:Glycoside Hydrolases; C023112:exo-beta-D-galactofuranosidase