Removal of bovine serum albumin using solid-phase extraction with in-situ polymerized stationary phase in a microfluidic device.
Serum albumin, one of the most abundant serum proteins, blocks the expression of other important biomarkers. The objective of this study is to remove serum albumin effectively by using solid-phase extraction (SPE) in microfluidic devices. Photo-polymerized adsorbent as a stationary phase of SPE was used to remove bovine serum albumin (BSA). The adsorption capacity was examined with the effect of pH and concentration in BSA solution, and adjustment of monomer concentration such as hydrophilic 2-acrylamido-2-methyl-1-propanesulfonic acid and acrylamide in the adsorbent. The effect of hydrophobic butyl methacylate on BSA adsorption was also studied. Selective removal in a bicomponent with BSA and bovine gamma-globulin was performed by adjusting the pH as required.
Lee
Eun Zoo
EZ
Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, South Korea.
Huh
Yun Suk
YS
Jun
Young-Si
YS
Won
Hyo Jin
HJ
Hong
Yeon Ki
YK
Park
Tae Jung
TJ
Lee
Sang Yup
SY
Hong
Won Hi
WH
D020106:Acrylamide / Q000528:radiation effects; D000178:Acrylamides / Q000528:radiation effects; D000327:Adsorption; D000476:Alkanesulfonates / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000528:radiation effects; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D008855:Microscopy, Electron, Scanning; D010777:Photochemistry; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*
D000178:Acrylamides; D000476:Alkanesulfonates; D008689:Methacrylates; D020106:Acrylamide; D012710:Serum Albumin, Bovine; C056288:2-acrylamido-2-methylpropanesulfonate; C004919:ethylene dimethacrylate; C016284:butyl methacrylate