[Preventive and therapeutic effects of Bushen Huoxue Recipe on autoimmune premature ovarian failure in mice].
OBJECTIVE
To investigate the preventive effect of Bushen Huoxue Recipe (BSHXR), a compound traditional Chinese herbal medicine, on autoimmune premature ovarian failure (POF) in mice.


METHODS
Ovarian antigen was prepared with the ovarian tissue of female BALB/c mice. A mouse model of POF was established by immunization injection of the ovarian antigen of isotype female mice on multiple subcutaneous sites and two posterior soles. The POF mice were treated with BSHXR after the first and third immunization. The levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) in peripheral blood were detected by enzyme linked immunosorbent assay.


RESULTS
Lymphocyte infiltration was appeared in ovarian stroma of POF mice. The levels of FSH and LH were evaluated and the E(2) level was decreased significantly (P<0.05). BSHXR could reduce the increased levels of FSH and LH, increase the level of E(2) and the number of growing and mature follicles. The efficacy of early treatment was better than that of late treatment.


CONCLUSIONS
BSHXR can recover ovarian function in POF mice mainly by regulating the indiscriminate hormone level, and BSHXR has preventive effect on autoimmune POF in mice.
Dong
Li
L
Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China. dongli888@yahoo.com
Jiang
Lin
L
Men
Wei
W
Zhu
Nan-sun
NS
D000818:Animals; D001327:Autoimmune Diseases / Q000188:drug therapy / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008517:Phytotherapy; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000188:drug therapy / Q000517:prevention & control*
D004365:Drugs, Chinese Herbal; C527851:bushen huoxue; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone