[Study on HPLC fingerprint of processed Radix Angelicae Dahuricae].
OBJECTIVE
To establish HPLC fingerprint analysis for the quality control of processed Radix Angelicae Dahuricae.


METHODS
HPLC fingerprint analysis of processed Radix Angelicae Dahuricae was developed Agilent C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 5% phosphorous acid mobile phase in a gradient mode. The flow rate was 1.0 mL/min. The wavelength of measurement was 254 nm. Fifteen batches of processed Radix Angelicae Dahuricae were determined.


RESULTS
The methodological evaluation showed that the method had a good repeatability.


CONCLUSIONS
The method can be used to identify and evaluate the quality of Radix Angelicae Dahuricae conveniently.
Zhang
Cui-ying
CY
Henan Provincial Institute for Food and Drug Control, Zhengzhou 450003, China.
Li
Zhen-guo
ZG
Wang
Qing-xiao
QX
D029969:Angelica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal