Evaluation of the Korean isolate-1 tachyzoite antigen for serodiagnosis of toxoplasmosis.
To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
Shin
Eun-Hee
EH
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Republic of Korea.
Kim
Dong-Hee
DH
Lin
Aifen
A
Lee
Jo-Woon-Yi
JW
Kim
Hyo-Jin
HJ
Ahn
Myoung-Hee
MH
Chai
Jong-Yil
JY
D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007723:Korea; D007841:Latex Fixation Tests; D008297:Male; D051379:Mice; D008875:Middle Aged; D011817:Rabbits; D012698:Serologic Tests; D014123:Toxoplasmosis / Q000097:blood / Q000175:diagnosis*
D000953:Antigens, Protozoan