title: Colorectal cancer progression correlates with upregulation of S100A11 expression in tumor tissues.
abstract: OBJECTIVE
  Early detection and treatment of human colorectal cancers remain a challenge. Identification of new potential markers may help in the diagnosis of colorectal cancer.
  
  
  METHODS
  By comparative two-dimensional gel electrophoresis using extracts from colorectal tumor and adjacent normal tissues, we identified a calcium-binding protein, S100A11, which was highly expressed in colorectal cancer compared with adjacent normal tissues. We expanded our study in 89 clinical colorectal tumor samples to validate this finding and correlates S100A11 expression in human colorectal cancer tissues with various stages of the tumor by Western blotting and immunohistochemical staining.
  
  
  RESULTS
  We identified a calcium-binding protein, S100A11, which was highly expressed in colorectal cancer compared with adjacent normal tissues. S100A protein was expressed predominantly in the cytoplasm of normal tissue; however, it was expressed in both the nuclei and cytoplasm of colorectal cancer. S100A11 level in colorectal cancer tissue was increased following stage progression of the disease.
  
  
  CONCLUSIONS
  These findings suggest S100A11 could be helpful in the pathological study of colorectal cancer, especially for the classification of different stages in colorectal cancer.
authors.affiliation: Department of Abdominal Surgery, The Affiliated Tumor Hospital, Harbin Medical University, Harbin 150086, China.
authors.forename: Guiyu
authors.initials: G
authors.lastname: Wang
authors.forename: Xishan
authors.initials: X
authors.lastname: Wang
authors.forename: Shuhuai
authors.initials: S
authors.lastname: Wang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Song
authors.forename: Haiming
authors.initials: H
authors.lastname: Sun
authors.forename: Weiguang
authors.initials: W
authors.lastname: Yuan
authors.forename: Bo
authors.initials: B
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.forename: Songbin
authors.initials: S
authors.lastname: Fu
mesh_terms: D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009367:Neoplasm Staging; D021381:Protein Transport; D009418:S100 Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D009418:S100 Proteins; C072337:S100A11 protein, human