[Method for DNA extraction and purification from corn-processed food using an ion-exchange resin type kit].
The yield of genomic DNA extracted from corn-processed foods, such as corn flake and one of the corn snack what is called "Jumbo corn", using an ion-exchange resin type kit (Gtip) has been reported to be very low, and it is thought to be difficult to detect the intrinsic corn gene "Zein" in the foods. Therefore, we developed a new method using Gtip, which we called the "KNG-Gtip method," by modification of the Ministry of Health, Labour and Welfare (MHLW) method using Gtip (MHLW-Gtip method). We compared the KNG-Gtip method, MHLW-Gtip method, the Gtip method for detection of allergen (ALG-Gtip method), and the Gtip method according to the Ministry of Agriculture, Forestry and Fisheries (MAFF) (JAS-Gtip method) in terms of the yield and quality of genomic DNA and the detection probabilities of the PCR-amplified Zein gene. The concentrations of DNA and the detection probabilities of the PCR-amplified Zein gene of genomic DNA extracted from 4 g corn flake and 4 g Jumbo corn by the KNG-Gtip method were larger than those by using the conventional methods. In addition, the PCR-amplified Zein gene from 4 g of corn starch could be detected by the KNG-Gtip method. We propose that the KNG-Gtip method, in which requires sample weight of four grams, is practical and useful to extract genomic DNA from corn flake and Jumbo corn.
Ohmori
Kiyomi
K
Kanagawa Prefectural Institute of Public Health, Kanagawa, Japan.
Tsuchiya
Hisayo
H
Watanabe
Takahiro
T
Akiyama
Hiroshi
H
Maitani
Tamio
T
Yamada
Toshiharu
T
Itoh
Shin-Ichi
S
Satoh
Shuji
S
D018744:DNA, Plant / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D007475:Ion Exchange Resins; D021141:Nucleic Acid Amplification Techniques; D003313:Zea mays / Q000235:genetics*
D018744:DNA, Plant; D007475:Ion Exchange Resins