title: Nitrogen depletion causes up-regulation of glutathione content and gamma-glutamyltranspeptidase in Schizosaccharomyces pombe.
abstract: This work aims to elucidate the relationship between nitrogen depletion and Glutathione (GSH) level in Schizosaccharomyces pombe. The total GSH level was much higher in the Pap1-positive KP1 cells than in the Pap1-negative TP108-3C cells, suggesting that synthesis of GSH is dependent on Pap1. When the Pap1-positive KP1 cells were transferred to the nitrogen-depleted medium, total GSH level significantly increased up to 6 h and then slightly declined after 9 h. Elevation of the total GSH level was observed to be much less with the Pap1-negative cells. However, glucose deprivation was not able to enhance the GSH level in the KP1 cells. Activity of gamma-glutamyltranspeptidase (gamma-GT), an enzyme in the first step of GSH catabolism, also increased during nitrogen depletion. The total GSH level was more significantly enhanced in the KP1 cells overexpressing gamma-GT2 than gamma-GT1 during nitrogen starvation. Reactive oxygen species (ROS) levels were not changed during nitrogen starvation in both Pap1-positive and Pap1-negative cells. Collectively, nitrogen depletion causes up-regulation of GSH synthesis and gamma-GT in a Pap1-dependent manner.
authors.affiliation: Division of Life Sciences and Research Institute of Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Song
authors.forename: Chang-Jin
authors.initials: CJ
authors.lastname: Lim
mesh_terms: D050976:Basic-Leucine Zipper Transcription Factors / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D008958:Models, Molecular; D009584:Nitrogen / Q000378:metabolism*; D000073718:Pancreatitis-Associated Proteins; D017382:Reactive Oxygen Species / Q000378:metabolism; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D015854:Up-Regulation; D005723:gamma-Glutamyltransferase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D017382:Reactive Oxygen Species; D029702:Schizosaccharomyces pombe Proteins; D005723:gamma-Glutamyltransferase; D005978:Glutathione; D009584:Nitrogen