In vitro neutralization of equid herpesvirus 1 mediated by recombinant antibodies.
A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
Molinková
D
D
Institute of Microbiology and Immunology, Faculty of Veterinary Medicine, Veterinary and Pharmaceutical University Brno, Palackého 1/3, 612 42 Brno, Czech Republic.
Skládal
P
P
Celer
V
V
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D006734:Horse Diseases / Q000276:immunology / Q000821:virology*; D006736:Horses; D015151:Immunoblotting; D007128:Immunoglobulin Fragments / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000276:immunology; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D019151:Peptide Library; D011994:Recombinant Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D010948:Viral Plaque Assay
D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1