Characterization of interleukin-1beta mRNA expression in chicken macrophages in response to avian reovirus.
Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1beta (IL-1beta) mRNA in chicken (chIL-1beta) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1beta mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1beta mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1beta mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1beta mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA(+) virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response.
Wu
Yung Fu
YF
Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan ROC.
Liu
Hung Jen
HJ
Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan ROC.
Shien
Jui Huang
JH
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan ROC.
Chiou
Shiow Her
SH
Graduate Institute of Veterinary Microbiology, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan ROC.
Lee
Long Huw
LH
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan ROC.
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001388:Aziridines / Q000494:pharmacology; D002478:Cells, Cultured; D002645:Chickens; D053583:Interleukin-1beta / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000378:metabolism*; D030101:Orthoreovirus, Avian / Q000187:drug effects; D012330:RNA, Double-Stranded / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000502:physiology; D012088:Reoviridae Infections / Q000378:metabolism*; D014771:Virion
D001388:Aziridines; D053583:Interleukin-1beta; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012367:RNA, Viral; C478655:binary ethyleneimine; D000643:Ammonium Chloride