Sanguibacter soli sp. nov., isolated from soil of a ginseng field.
A Gram-positive, non-spore-forming, rod-shaped, motile bacterium, designated strain DCY22(T), was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY22(T) belonged within the family Sanguibacteraceae, and highest levels of sequence similarity were found with Sanguibacter marinus 1-19(T) (96.8 %), Sanguibacter suarezii ST-26(T) (96.0 %), Sanguibacter inulinus ST-50(T) (95.9 %), Sanguibacter keddieii ST-74(T) (95.5 %), Terrabacter terrae PPLB(T) (94.0 %) and Terrabacter tumescens DSM 20308(T) (93.8 %). Chemotaxonomic investigations revealed that strain DCY22(T) possessed menaquinone MK-9, a common feature of members of the genus Sanguibacter. Predominant fatty acids were unknown ECL 13.961 (45.81 %), 17 : 0 anteiso (23.46 %), 18 : 0 iso (15.42 %) and unknown ECL 14.966 (8.70 %). The results of physiological and biochemical tests clearly demonstrated that strain DCY22(T) represents a novel species of the genus Sanguibacter, for which the name Sanguibacter soli sp. nov. is proposed. The type strain is DCY22(T) (=KCTC 13155(T)=JCM 14841(T)).
Kim
Myung Kyum
MK
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Kiheung-gu, Yongin, Kyunggi-do 449-701, Republic of Korea.
Pulla
Rama Krishna
RK
Kim
Se-Young
SY
Yi
Tae-Hoo
TH
Soung
Nak-Kyun
NK
Yang
Deok-Chun
DC
D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D005894:Panax / Q000254:growth & development*; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S