PASADENA hyperpolarization of succinic acid for MRI and NMR spectroscopy.
We use the PASADENA (parahydrogen and synthesis allow dramatically enhanced nuclear alignment) method to achieve 13C polarization of approximately 20% in seconds in 1-13C-succinic-d2 acid. The high-field 13C multiplets are observed as a function of pH, and the line broadening of C1 is pronounced in the region of the pK values. The 2JCH, 3JCH, and 3JHH couplings needed for spin order transfer vary with pH and are best resolved at low pH leading to our use of pH approximately 3 for both the molecular addition of parahydrogen to 1-13C-fumaric acid-d2 and the subsequent transfer of spin order from the nascent protons to C1 of the succinic acid product. The methods described here may generalize to hyperpolarization of other carboxylic acids. The C1 spin-lattice relaxation time at neutral pH and 4.7 T is measured as 27 s in H2O and 56 s in D2O. Together with known rates of succinate uptake in kidneys, this allows an estimate of the prospects for the molecular spectroscopy of metabolism.
Chekmenev
Eduard Y
EY
A. A. Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena, California 91125, USA.
Hövener
Jan
J
Norton
Valerie A
VA
Harris
Kent
K
Batchelder
Lynne S
LS
Bhattacharya
Pratip
P
Ross
Brian D
BD
Weitekamp
Daniel P
DP
D000465:Algorithms; D002247:Carbon Isotopes; D003903:Deuterium; D006863:Hydrogen-Ion Concentration; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards; D008968:Molecular Conformation; D012015:Reference Standards; D012680:Sensitivity and Specificity; D019802:Succinic Acid / Q000737:chemistry*
D002247:Carbon Isotopes; D019802:Succinic Acid; D003903:Deuterium