Determination of oxatomide in human plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry.
A rapid, sensitive and specific high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method has been developed and validated for the determination of oxatomide in human plasma. Flunarizine hydrochloride was employed as the internal standard (IS). The analytes were chromatographically separated on a Shimadzu Shim-pack VP-ODS C18 column (250 x 2.0 mm i.d.) with a mobile phase consisting of methanol and aqueous ammonium acetate solution (10 mm, pH 4.0; 85:15, v/v). Detection was performed on a single quadrupole mass spectrometer using an electrospray ionization interface with the selected-ion monitoring (SIM) mode. The method showed excellent linearity (r = 0.9995) over the concentration range of 0.5-500 ng/mL with good accuracy and precision. The intra- and inter-batch precisions were within 10% relative standard deviation. The recoveries were more than 90%. The validated method was successfully applied to a preliminary pharmacokinetic study of oxatomide in Chinese healthy male volunteers.
She
Xiaodong
X
Department of Pharmacology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, People's Republic of China.
Wu
Lili
L
Wei
Huilin
H
Liu
Weida
W
Chen
Yun
Y
D000284:Administration, Oral; D018926:Anti-Allergic Agents / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005444:Flunarizine / Q000097:blood; D006634:Histamine H1 Antagonists / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D016014:Linear Models; D008297:Male; D010879:Piperazines / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D018926:Anti-Allergic Agents; D006634:Histamine H1 Antagonists; D010879:Piperazines; C015408:oxatomide; D005444:Flunarizine