Real-time viability assay based on 51Cr retention in adherent cells.
The chromium (51Cr) release assay has been widely used for viability measurements, even though it has major disadvantages such as high manual workload and poor time resolution. By the use of LigandTracer 51Cr release viability measurements on adherent cells can be significantly simplified and improved. LigandTracer enables a time-resolved detection of 5SCr in target cells, with the result that the effect of toxic material is updated continuously throughout the experiment. Here we explain the principle behind this novel real-time viability assay and show viability curves for known toxic compounds on A431 and U343MGaCl2:6 cell lines.
Vennström
Lina
L
Ridgeview Instruments AB, Uppsala.
Bysell
Camilla
C
Björkelund
Hanna
H
Lundqvist
Hans
H
Andersson
Karl
K
D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002857:Chromium / Q000378:metabolism*; D002860:Chromium Radioisotopes; D019327:Copper Sulfate / Q000633:toxicity; D006801:Humans; D017830:Octoxynol / Q000633:toxicity; D018675:Toxicity Tests / Q000379:methods*
D002860:Chromium Radioisotopes; D002857:Chromium; D017830:Octoxynol; D019327:Copper Sulfate