Application of a new bioassay technique using goldfish for assessment of water toxicity.
There are a variety of chemicals in aquatic environment, so it is important to assess the toxicity. The biomarkers such as induction of DNA damage, micronuclei, vitellogenin, and hepatic P450 in fish are known to be effective for monitoring genotoxic and/or estrogenic chemicals. However, there is little study to use these biomarkers in same fish. Goldfish (Carassius auratus) is widely used and is suitable in size to collect blood or organs. In this study, validity of multiple-biomarkers in goldfish was checked using standard chemicals and applied in the river water. Ho River, which flows through the textile dyeing factory in Shizuoka Prefecture, Japan, was reported to show genotoxicity toward Salmonella typhimurium TA98 and YG1024. When the goldfish were exposed to Ho River, DNA damage, estrogenic activity, and CYP1A induction were observed. Through the study, it was assumed that not only mutagens/carcinogens but also endocrine disrupting chemicals and poly aromatic hydrocarbons were present in Ho River. Therefore, chemical identification should be required. We could evaluate both genotoxicity and estrogenic activity simultaneously, so goldfish might be a good experimental model for estimation of chemical contamination levels in aquatic environment.
Deguchi
Yuya
Y
Graduate School of Nutritional and Environmental Sciences, COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. deguchi@niu.ac.jp
Wu
Nan Xiang
NX
Toyoizumi
Tomoyasu
T
Masuda
Shuichi
S
Nagaoka
Hiroaki
H
Watanabe
Tetsushi
T
Totsuka
Yukari
Y
Wakabayashi
Keiji
K
Kinae
Naohide
N
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D015197:Carcinogenicity Tests; D004396:Coloring Agents / Q000378:metabolism / Q000633:toxicity; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity; D004967:Estrogens / Q000378:metabolism; D005618:Fresh Water; D006054:Goldfish / Q000378:metabolism*; D007220:Industrial Waste; D007564:Japan; D009152:Mutagenicity Tests; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism / Q000633:toxicity; D018570:Risk Assessment; D013783:Textile Industry; D018675:Toxicity Tests / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D004396:Coloring Agents; D052244:Endocrine Disruptors; D004967:Estrogens; D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1