title: Femtosecond spectroscopy of native and carotenoidless purple-bacterial LH2 clarifies functions of carotenoids.
abstract: EET between the two circular bacteriochlorophyll compartments B800 and B850 in native (containing the carotenoid rhodopin) and carotenoidless LH2 isolated from the photosynthetic purple sulfur bacterium Allochromatium minutissimum was investigated by femtosecond time-resolved transient absorption spectroscopy. Both samples were excited with 120-fs laser pulses at 800 nm, and spectral evolution was followed in the 720-955 nm range at different delay times. No dependence of transient absorption in the B800 band on the presence of the carotenoid rhodopin was found. Together with the likewise virtually unchanged absorption spectra in the bacteriochlorophyll Q(y) region, these observations suggest that absence of rhodopin does not significantly alter the structure of the pigment-protein complex including interactions between bacteriochlorophylls. Apparently, rhodopin does also not accelerate B800 to B850 EET in LH2, contrary to what has been suggested previously. Moreover, "carotenoid-catalyzed internal conversion" can also be excluded for the bacteriochlorophylls in LH2 of A. minutissimum. Together with previous results obtained with two-photon fluorescence excitation spectroscopy, it can also be concluded that there is neither EET from rhodopin to B800 nor (back-)EET from B800 to rhodopin.
authors.affiliation: Institut für Optik und Atomare Physik, Technische Universität Berlin, 10623 Berlin, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Theiss
authors.forename: Dieter
authors.initials: D
authors.lastname: Leupold
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Moskalenko
authors.forename: Andrei P
authors.initials: AP
authors.lastname: Razjivin
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Eichler
authors.forename: Heiko
authors.initials: H
authors.lastname: Lokstein
mesh_terms: D002338:Carotenoids / Q000737:chemistry* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D007700:Kinetics; D008027:Light; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000528:radiation effects*; D020560:Proteobacteria / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D013057:Spectrum Analysis
chemical_list: D045342:Light-Harvesting Protein Complexes; D002338:Carotenoids