Stir-baked Fructus gardeniae (L.) extracts inhibit matrix metalloproteinases and alter cell morphology.
Matrix metalloproteinases (MMPs) play vital roles in many pathological conditions, including cancer, cardiovascular disease, arthritis and inflammation. Modulating MMP activity may therefore be a useful therapeutic approach in treating these diseases. Qing-Kai-Ling is a popular Chinese anti-inflammatory formulation used to treat symptoms such as rheumatoid arthritis, acute hypertensive cerebral hemorrhage, hepatitis and upper respiratory tract infection. In this paper, we report that one of the components of Qing-Kai-Ling, Fructus gardeniae, strongly inhibits MMP activity. The IC50 values for the primary herbal extract and water extract against MMP-16 were 32 and 27 microg/ml, respectively. In addition, we show that the herbal extracts influence HT1080 human fibrosarcoma cell growth and morphology. These data may provide molecular mechanisms for the therapeutic effects of Qing-Kai-Ling and herbal medicinal Fructus gardeniae.
Yang
Jin-gang
JG
Key laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, Jilin University, Changchun 130023, PR China.
Shen
Ye-hua
YH
Hong
Yuan
Y
Jin
Feng-hai
FH
Zhao
Shu-hua
SH
Wang
Ming-cui
MC
Shi
Xiu-juan
XJ
Fang
Xue-xun
XX
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D032062:Gardenia / Q000737:chemistry*; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011480:Protease Inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles
D004365:Drugs, Chinese Herbal; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; C504928:Qingkailing; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue