Novel Rath peptide for intracellular delivery of protein and nucleic acids.
In the present study, a novel cell penetrating peptide (CPP) named as Rath, has been identified from the avian infectious bursal disease virus. It has the potential to penetrate and translocate cargo molecules into cells independent of temperature. Additionally, it can deliver oligonucleotide in 30min and antibodies within an hour intracellular to chicken embryonic fibroblast primary cells. As an ideal delivery vehicle, it has the ability to protect the cargo molecules in the presence of serum, nucleases and has minimal or no cytotoxicity at even higher peptide concentrations studied. The biophysical characterizations showed that Rath has a dominant beta structure with a small alpha helix and has remarkable binding ability with protein and DNA. Thus, the characterization of unique Rath peptide to deliver protein or nucleic acid into the cells with non-covalent interaction could be used as an effective delivery method for various cell based assays.
Bais
Manish V
MV
Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, UP, India. manish.bais@gmail.com
Kumar
Satish
S
Tiwari
Ashok K
AK
Kataria
Ranjit S
RS
Nagaleekar
Viswas K
VK
Shrivastava
Sameer
S
Chindera
Kantaraja
K
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001692:Biological Transport; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage* / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005347:Fibroblasts / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D010957:Plasmids / Q000378:metabolism; D017433:Protein Structure, Secondary; D021381:Protein Transport; D011506:Proteins / Q000008:administration & dosage*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity
D000906:Antibodies; D009841:Oligonucleotides; D010455:Peptides; D011506:Proteins; C518635:VP5 protein, infectious bursal disease virus; D017361:Viral Nonstructural Proteins; D004247:DNA