Poly(imidazole/DMAEA)phosphazene/DNA self-assembled nanoparticles for gene delivery: synthesis and in vitro transfection.
A new cationic derivate of polyphosphazene with imidazole and 2-dimethylaminoethylamino (DMAEA) as side groups, poly(imidazole/DMAEA)phosphazene (PIDP), was synthesized and investigated for gene delivery. The half-lives of PIDP degradation under neutral (pH 7.4) and acidic conditions (pH 5.0) were 22 and 3 days at 37 degrees C, respectively. The cytotoxicity of PIDP assayed by MTT was much lower than that of poly(2-dimethylaminoethylamino)phosphazene (PDAP) and PEI 25K. PIDP could condense DNA into nanoparticles with a size around 100 nm and zeta potential (+25 mV) at the ratio of 10:1 (PIDP/DNA, w/w). The transfection efficiency of PIDP/DNA complex nanoparticles (PICNs) against 293T, COS-7 and Hela cells was much higher than that of PDAP/DNA complexes nanoparticles (PDCNs) and PEI/DNA complexes nanoparticles (PECNs) at 10:1 (polymer/DNA, w/w). Therefore, PIDP could be a safe, efficient and promising cationic polymer for gene therapy.
Yang
Yongxin
Y
Center for Drug Delivery System, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Xu
Zhenghong
Z
Jiang
Jingui
J
Gao
Yu
Y
Gu
Wangwen
W
Chen
Lingli
L
Tang
Xiaozheng
X
Li
Yaping
Y
D000818:Animals; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007093:Imidazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014162:Transfection / Q000379:methods*
D007093:Imidazoles; D009943:Organophosphorus Compounds; D011108:Polymers; C485184:enhanced green fluorescent protein; C530090:poly(imidazole-2-dimethylaminoethylamino)phosphazene; C108974:poly(phosphazene); D049452:Green Fluorescent Proteins; D004247:DNA