Construction of a stress-induced system in Escherichia coli for efficient polyhydroxyalkanoates production.
In the application of engineered Escherichia coli in industrial polyhydroxybutyrate production process, one of the major concerns is the induction of the metabolic pathway. In this study, we developed a stress-induced system by which the PHB biosynthesis pathways can be induced under stress conditions. Fermentation results showed that recombinant E. coli DH5alpha (pQKZ103) harboring this system was able to accumulate polyhydroxybutyrate up to 85.8% of cell dry weight in minimal glucose medium without adding any inducer. Growth experiment with GFP as a reporter indicated that the induction of this system happened at the late exponential phase and was sensitive to stressed environment. This system can also be applied in many other biotechnological processes.
Kang
Zhen
Z
State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Jinan 250100, People's Republic of China.
Wang
Qian
Q
Zhang
Haojun
H
Qi
Qingsheng
Q
D003470:Culture Media / Q000378:metabolism; D004738:Engineering / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods; D011995:Recombination, Genetic
D003470:Culture Media; D054813:Polyhydroxyalkanoates; D005947:Glucose