Optimal aggregation of dissociated islet cells for functional islet-like cluster.
Genetically-engineered islets can serve as a potential means to modulate the regional immunity occurring in the transplant environment, although gene transfer into islets is not easy due to the extracellular matrix of islets. In this study, we aggregated single islet cells dissociated by trypsinization for successful gene transfection into islet cells. To enhance the functionality of aggregated islets, we optimized the conditions for the aggregation of dissociated islet cells using several supplements such as collagen, all-trans-retinoic acid (atRA) and the culture medium of pancreatic exocrine cells (ECM). The dissociated islet cells formed tight cell clusters containing the normal insulin secretion against glucose concentration and the aggregation yield was significantly improved by treatment with atRA and ECM. However, collagen did not improve cell aggregation. Therefore, this re-aggregation technology would be useful for the development of genetically-engineered islets.
Lee
Dong Yun
DY
College of Pharmacy, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul 151-742, South Korea.
Park
Sang Jin
SJ
Nam
Jong Hee
JH
Byun
Youngro
Y
D002449:Cell Aggregation; D003094:Collagen / Q000378:metabolism; D003470:Culture Media; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D046790:Pancreas, Exocrine / Q000166:cytology / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism
D003470:Culture Media; D007328:Insulin; D014212:Tretinoin; D003094:Collagen