title: Effects of the herbicide isopropyl-N-phenyl carbamate on microtubules and MTOCs in lines of Nicotiana sylvestris resistant and sensitive to its action.
abstract: To clarify the mechanism of isopropyl-N-phenyl carbamate (IPC) action on higher plant cells the sensitivity of microtubules (cortical network and mitotic arrays) and microtubule organizing centers to IPC treatment (30 microM) in IPC-resistant and sensitive Nicotiana sylvestris lines was studied. It was clearly demonstrated that IPC does not depolymerize plant MTs but causes the MTOC damage in cells, which results in MTOC fragmentation, splitting of the spindle poles and in abnormal division spindle formation. It was also found that IPC-resistance of mutant N. sylvestris line correlates not with tubulin resistance to IPC action but possibly with resistance of one of the proteins involved in MTOC composition.
authors.affiliation: Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine. alyemets@univ.kiev.ua
authors.forename: Alla
authors.initials: A
authors.lastname: Yemets
authors.forename: Oksana
authors.initials: O
authors.lastname: Stelmakh
authors.forename: Yaroslav B
authors.initials: YB
authors.lastname: Blume
mesh_terms: D053209:Herbicide Resistance; D006540:Herbicides / Q000633:toxicity*; D022101:Microtubule-Organizing Center / Q000187:drug effects* / Q000648:ultrastructure; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D048448:Phenylcarbamates / Q000633:toxicity*; D014026:Nicotiana / Q000187:drug effects* / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis
chemical_list: D006540:Herbicides; D048448:Phenylcarbamates; D014404:Tubulin; C100175:propham