A protocol for a lung neovascularization model in rodents.
By providing insight into the cellular events of vascular injury and repair, experimental model systems seek to promote timely therapeutic strategies for human disease. The goal of many current studies of neovascularization is to identify cells critical to the process and their role in vascular channel assembly. We propose here a protocol to analyze, in an in vivo rodent model, vessel and capillary remodeling (reorganization and growth) in the injured lung. Sequential analyses of stages in the assembly of vascular structures, and of relevant cell types, provide further opportunities to study the molecular and cellular determinants of lung neovascularization.
Jones
Rosemary C
RC
Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, Massachusetts 02114, USA. rcjones@partners.org
Capen
Diane
D
Petersen
Bodil
B
Jain
Rakesh K
RK
Duda
Dan G
DG
D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D018496:Hyperoxia / Q000473:pathology; D008168:Lung / Q000098:blood supply*; D055370:Lung Injury; D008297:Male; D051379:Mice; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009584:Nitrogen / Q000008:administration & dosage; D010100:Oxygen / Q000008:administration & dosage; D051381:Rats
D009584:Nitrogen; D010100:Oxygen