title: [Preliminary study of protein expression profiling of PCOS on different state].
abstract: OBJECTIVE
  To screen the serum protein expression profiles in patients having polycystic ovary syndrome (PCOS) with or without insulin resistance (IR) and search for discriminatory proteins.
  
  
  METHODS
  Fasting serum samples of 30 PCOS patients with IR, 30 PCOS patients without IR, and 30 control individuals from Reproductive Center of Peking University Third Hospital were studied.
  
  
  RESULTS
  There were 27 differential protein peaks between PCOS IR patients and controls, 17 between PCOS non-IR patients and controls, and 19 between PCOS IR patients and non-IR patients. Marker proteins from differentially expressed proteins were screened out using support vector machine (SVM), and were used to establish three diagnostic models for PCOS IR, PCOS non-IR, and IR, respectively.
  
  
  CONCLUSIONS
  There were significantly different serum proteomic patterns in different types of PCOS. Using Protein Chip combined with SVM, computer diagnostic models for PCOS with and without IR were set up quickly and efficiently. These discriminatory proteins may help us understand the proteomic changes in serum and find out potential biomarkers of PCOS and IR.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100083, China.
authors.forename: Shu-Yun
authors.initials: SY
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
authors.forename: Mei-Zhi
authors.initials: MZ
authors.lastname: Li
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Jie-Kai
authors.initials: JK
authors.lastname: Yu
authors.forename: Rong
authors.initials: R
authors.lastname: Li
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000503:physiopathology; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins