title: [Development and evaluation of a double antigen sandwich ELISA for the detection of total antibodies against hemorrhagic fever with renal syndrome virus].
abstract: OBJECTIVE
  To develop and evaluate a method for detection of the total antibodies against hemorrhagic fever with renal syndrome (HFRS) virus with improved sensitivity and simplified operation procedure.
  
  
  METHODS
  The nucleic proteins of hantavirus were used as coating antigens as well as detection antigens labeled with horse radish peroxidase (HRP). The operation protocol was established, optimized and compared with indirect fluorescence assay (IFA).
  
  
  RESULTS
  The specificity of this method was 100 percent in the test of different human sera and 4-8 times more sensitive than IFA. And, it is simpler without requiring any change of the reagents, different sources of samples did not affect the results of the test.
  
  
  CONCLUSIONS
  This method is specific, sensitive and simple for detection of the total antibodies in sera against hantavirus, could be used for the screening of Hantavirus infection in human and host rodent animals.
authors.affiliation: State Key Laboratory for Infectious Diseases, Institute for Viral Diseases Control and Prevention, Beijing 100052, China.
authors.forename: Quan-fu
authors.initials: QF
authors.lastname: Zhang
authors.forename: Jian-dong
authors.initials: JD
authors.lastname: Li
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Li
authors.forename: Chuan
authors.initials: C
authors.lastname: Li
authors.forename: Qin-zhi
authors.initials: QZ
authors.lastname: Liu
authors.forename: Mi-fang
authors.initials: MF
authors.lastname: Liang
authors.forename: De-xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D018063:Hantaan virus / Q000276:immunology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral