Genotypic analysis of Candida albicans isolates obtained from removable prosthesis wearers.
OBJECTIVE
To assess of the genotypic diversity of Candida albicans isolated from removable prosthesis wearers, with and without denture-related stomatitis (DRS). The occurrence of different genotypes in pathological and control cases was investigated.


RESULTS
One hundred and sixty-four isolates of C. albicans obtained from different oral cavity locations were compared by randomly amplified polymorphic DNA (RAPD). The coherence of this analysis was confirmed by genotyping a selected group of isolates with pulsed field gel electrophoresis (PFGE). Among the 164 isolates, 150 were grouped into seven groups on the basis of their RAPD patterns. Three of these groups (comprising 54 isolates) had significant (alpha < 0.10) predominance of clinical or control cases. For the other isolates, no significant differences were observed between control and DRS cases. Occasionally, more than one genotype was found in the same person. These findings were sustained by PFGE analysis. No relevant associations between the genotypic patterns and pathology level were found.


CONCLUSIONS
This study evidenced that C. albicans with similar genotypes may be found in individuals with DRS and in control cases.


CONCLUSIONS
This conclusion hints the involvement of other aetiological factors that alone or in association with C. albicans may trigger the emergence of DRS.
Costa
F
F
Escola Superior de Biotecnologia da Universidade Católica Portuguesa, Porto, Portugal.
Manaia
C M
CM
Figueiral
M H
MH
Pinto
E
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002176:Candida albicans / Q000145:classification* / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D003778:Dentures / Q000009:adverse effects*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D010447:Peptide Hydrolases / Q000032:analysis; D010740:Phospholipases / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique; D013282:Stomatitis, Denture / Q000382:microbiology*
D004271:DNA, Fungal; D010740:Phospholipases; D010447:Peptide Hydrolases