title: The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection.
abstract: Acute rejection remains a poor predictor of graft outcome. In this study, we measured serum levels of interferon (IFN)-gamma and neopterin by enzyme-linked immunosorbent assay and a single nucleotide polymorphism (SNP) within the 3' untranslated region of the interleukin (IL)-12 B gene (1188 A/C) to determine whether either of these factors could predict acute rejection in renal transplantation. Significantly higher early post-transplant neopterin levels (days 5-7; 35.7 versus 19.9 nmol/l) were observed in recipients who subsequently rejected their grafts. Post-transplant neopterin levels showed a strong positive correlation with 1-month creatinine levels (Spearman's correlation 0.62, P < 0.001), suggesting macrophage activation early after transplantation. Pretransplant neopterin and IFN-gamma levels and the IL-12B gene SNP did not predict acute rejection in this small retrospective study. The ability to predict acute rejection non-invasively early after transplantation could lead to individual tailoring of immunosuppressive regimens and perhaps lead eventually to longer graft survival.
authors.affiliation: South-west Transplant Centre, Derriford Hospital, Plymouth, UK.
authors.forename: G K
authors.initials: GK
authors.lastname: Chin
authors.forename: C L
authors.initials: CL
authors.lastname: Adams
authors.forename: B S
authors.initials: BS
authors.lastname: Carey
authors.forename: S
authors.initials: S
authors.lastname: Shaw
authors.forename: W-Y
authors.initials: WY
authors.lastname: Tse
authors.forename: E R
authors.initials: ER
authors.lastname: Kaminski
mesh_terms: D000208:Acute Disease; D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D019798:Neopterin / Q000097:blood*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012189:Retrospective Studies
chemical_list: D015415:Biomarkers; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40; D019798:Neopterin; D007371:Interferon-gamma