title: Preparation and characterization of nano-hydroxyapatite/silk fibroin porous scaffolds.
abstract: Novel tissue engineering scaffold materials of nano-hydroxyapatite (nHA)/silk fibroin (SF) biocomposite were prepared by freeze-drying. The needle-like nHA crystals of about 10 nm in diameter by 50-80 nm in length, which were uniformly distributed in the porous nHA/SF scaffolds, were prepared by a co-precipitation method with a size. The as-prepared nHA/SF scaffolds showed good homogeneity, interconnected pores and high porosity. XRD and FT-IR analysis suggested that the silk fibroin was in beta-sheet structure, which usually provides outstanding mechanical properties for silk materials. In this work, composite scaffolds containing as high as 70% (w/w) nHA were prepared, which had excellent compressive modulus and strength, higher than the scaffolds at low nHA content level and other porous biodegradable polymeric scaffolds often considered in bone-related tissue engineering reported previously. The cell compatibility of composite scaffolds was evaluated through cell viability by MTT assay. All these results indicated that these nHA/SF scaffold materials may be a promising biomaterial for bone tissue engineering.
authors.affiliation: The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textile, Zhejiang Sci-Tech University, Xiasha Higher Education Park, Hangzhou, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Jinying
authors.initials: J
authors.lastname: Liu
authors.forename: Mingqi
authors.initials: M
authors.lastname: Wang
authors.forename: Sijia
authors.initials: S
authors.lastname: Min
authors.forename: Yurong
authors.initials: Y
authors.lastname: Cai
authors.forename: Liangjun
authors.initials: L
authors.lastname: Zhu
authors.forename: Juming
authors.initials: J
authors.lastname: Yao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D012831:Bombyx; D045744:Cell Line, Tumor; D018360:Crystallography, X-Ray; D017886:Durapatite / Q000138:chemical synthesis / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D013696:Temperature; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D005349:Fibroins; D017886:Durapatite