Lipid metabolism in liver of rat exposed to cadmium.
We investigated the effect of exposition to cadmium (Cd, 15ppm for 8 weeks) through drinking water on liver lipid metabolism in adult male Wistar rats. As compared to metal non-exposed (control) rats, the serum triglycerides, cholesterol and LDL+VLDL cholesterol concentrations increased. This was associated to a decrease of lipoprotein lipase activity in post heparinic plasma. The VLDL secretion from liver was not modified. Cd treatment increased triglycerides and decreased esterified cholesterol contents in liver. The high triglyceride mass was related to the increased glycerol-3-phosphate acyltransferase mRNA expression. In addition, the liver fatty acids synthesis increased, as determined by an increment of fatty acid synthetase and isocitrate dehydrogenase activities, and [(14)C]-acetate incorporation into saponifiable lipid fraction. The relative percentage of palmitic acid (16:0) and total saturated fatty acids were increased compared with control. Hepatic glucose-6-phosphate dehydrogenase, malic dehydrogenase and cholesteryl ester hydrolase activities were unchanged. In liver, the Cd treatment decreased triglyceride and cholesterol in mitochondria, also increased triglyceride in cytosol, and cholesterol and phospholipid contents in nuclei, compared with control. In addition, an increase of nuclei phosphatidylcholine synthesis was observed. Cd exposure alters directly or indirectly the serum lipid content and liver lipid metabolism.
Larregle
Ethel V
EV
Department of Biochemistry and Biological Sciences, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, IMBIO-SL, CONICET, San Luis, Argentina.
Varas
Silvia M
SM
Oliveros
Liliana B
LB
Martinez
Luis D
LD
Antón
Rosa
R
Marchevsky
Eduardo
E
Giménez
María S
MS
D000085:Acetates / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002104:Cadmium / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D002794:Choline / Q000031:analogs & derivatives / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008071:Lipoprotein Lipase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010743:Phospholipids / Q000097:blood; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013054:Spectrophotometry, Atomic; D013109:Sphingomyelins / Q000378:metabolism; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D000085:Acetates; D015243:Cholesterol, VLDL; D005227:Fatty Acids; D010743:Phospholipids; D012333:RNA, Messenger; D013109:Sphingomyelins; D014280:Triglycerides; C017506:methylcholine; D002104:Cadmium; D002784:Cholesterol; D008071:Lipoprotein Lipase; D002794:Choline