Supplementation with galactooligosaccharides and inulin increases bacterial translocation in artificially reared newborn rats.
Supplementation of formulas with prebiotics enhances the growth of lactate producing bacteria, and fecal lactate, and acetate levels in infants. High concentrations of organic acids in intestinal lumen have, however, been shown to impair the intestinal barrier function. To determine whether stimulating the colonic microbiotal metabolism with prebiotics would impair the neonatal intestinal barrier function, artificially reared rats were fed milk formula with or without a mixture of galactooligosaccharides/inulin (GOS/Inulin, 88/12; 5.6 g/L) from the 7th d of life (d7) until weaning (d20). At d18, GOS/inulin supplementation had increased the concentrations of acetate and lactate in colonic lumen. Although neither ileum-associated microbiota nor colonic permeability (assessed in Ussing chambers), nor the expression of tight junction claudin-2 and claudin-3 mRNA were altered, GOS/inulin supplementation was associated with increased bacterial translocation (BT) toward spleen. None of these effects persisted at d40. We conclude that GOS/inulin supplementation may increase BT in an immature gut. The balance between the potential infectious risk of BT vs. its putative beneficial effect on the maturation of neonatal immune system clearly warrants further study.
Barrat
Emmanuel
E
UMR 1280, Physiologie des Adaptations Nutritionnelles, Université de Nantes, Nantes, F-44093, France.
Michel
Catherine
C
Poupeau
Guillaume
G
David-Sochard
Agnes
A
Rival
Martine
M
Pagniez
Anthony
A
Champ
Martine
M
Darmaun
Dominique
D
D019342:Acetic Acid / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D018988:Bacterial Translocation / Q000187:drug effects*; D003106:Colon / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D004750:Enteral Nutrition; D005526:Food, Formulated / Q000009:adverse effects; D007082:Ileum / Q000187:drug effects / Q000382:microbiology; D007444:Inulin / Q000008:administration & dosage* / Q000009:adverse effects; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D009844:Oligosaccharides / Q000008:administration & dosage* / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014886:Weaning
D009844:Oligosaccharides; D019344:Lactic Acid; D007444:Inulin; D019342:Acetic Acid