title: A complete collection of single-gene deletion mutants of Acinetobacter baylyi ADP1.
abstract: We have constructed a collection of single-gene deletion mutants for all dispensable genes of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were obtained, whereas 499 (16%) were not, and are therefore candidate essential genes for life on minimal medium. This essentiality data set is 88% consistent with the Escherichia coli data set inferred from the Keio mutant collection profiled for growth on minimal medium, while 80% of the orthologous genes described as essential in Pseudomonas aeruginosa are also essential in ADP1. Several strategies were undertaken to investigate ADP1 metabolism by (1) searching for discrepancies between our essentiality data and current metabolic knowledge, (2) comparing this essentiality data set to those from other organisms, (3) systematic phenotyping of the mutant collection on a variety of carbon sources (quinate, 2-3 butanediol, glucose, etc.). This collection provides a new resource for the study of gene function by forward and reverse genetic approaches and constitutes a robust experimental data source for systems biology approaches.
authors.affiliation: Genoscope, Institut de Génomique (CEA), Evry, France. vberard@genoscope.fr
authors.forename: Véronique
authors.initials: V
authors.lastname: de Berardinis
authors.forename: David
authors.initials: D
authors.lastname: Vallenet
authors.forename: Vanina
authors.initials: V
authors.lastname: Castelli
authors.forename: Marielle
authors.initials: M
authors.lastname: Besnard
authors.forename: Agnès
authors.initials: A
authors.lastname: Pinet
authors.forename: Corinne
authors.initials: C
authors.lastname: Cruaud
authors.forename: Sumitta
authors.initials: S
authors.lastname: Samair
authors.forename: Christophe
authors.initials: C
authors.lastname: Lechaplais
authors.forename: Gabor
authors.initials: G
authors.lastname: Gyapay
authors.forename: Céline
authors.initials: C
authors.lastname: Richez
authors.forename: Maxime
authors.initials: M
authors.lastname: Durot
authors.forename: Annett
authors.initials: A
authors.lastname: Kreimeyer
authors.forename: François
authors.initials: F
authors.lastname: Le Fèvre
authors.forename: Vincent
authors.initials: V
authors.lastname: Schächter
authors.forename: Valérie
authors.initials: V
authors.lastname: Pezo
authors.forename: Volker
authors.initials: V
authors.lastname: Döring
authors.forename: Claude
authors.initials: C
authors.lastname: Scarpelli
authors.forename: Claudine
authors.initials: C
authors.lastname: Médigue
authors.forename: Georges N
authors.initials: GN
authors.lastname: Cohen
authors.forename: Philippe
authors.initials: P
authors.lastname: Marlière
authors.forename: Marcel
authors.initials: M
authors.lastname: Salanoubat
authors.forename: Jean
authors.initials: J
authors.lastname: Weissenbach
mesh_terms: D000150:Acinetobacter / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D002244:Carbon / Q000378:metabolism; D002874:Chromosome Mapping; D003470:Culture Media; D017931:DNA Primers / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D008954:Models, Biological; D008957:Models, Genetic; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D049490:Systems Biology
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D017931:DNA Primers; D002244:Carbon