Glycoforms of CA125 antigen as a possible cancer marker.
CA125, a coelomic epithelium-related antigen, is expressed in both normal and pathological conditions. In this study, we compared the glycosylation of CA125 antigen from amniotic fluid and the ovarian carcinoma cell line OVCAR-3, in order to detect possible differences as a specific marker of their origin. Antigens from both sources were radiolabelled and subsequently subjected to the affinity chromatography, using plant lectins differing in carbohydrate specificity as ligands. A common chromatographic scheme was applied to all columns, i.e. they were eluted with: a) washing buffer to wash out non-bound and low-affinity bound fractions, b) a solution of inhibitory sugar and c) a low pH buffer, to release the high affinity bound fractions. CA125 antigen from each source was found to be heterogeneous in respect to the existence of multiple glycoforms, with O-linked glycan chains predominating. However, the binding patterns of both N- and O-linked glycan-reactive lectins indicated distinct differences in carbohydrate composition between CA125 antigen isolated from amniotic fluid and OVCAR-3 cell line. The observed specificites of CA125-oligosaccharide chains might be of special importance from the biomedical aspect, in terms of their possible use for clinical evaluation of gynecological functions in health and disease.
Jankovic
Miroslava M
MM
Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Banatska 31b, 11080 Zemun, Serbia. miraj@inep.co.yu
Milutinovic
Bojana S
BS
D014408:Biomarkers, Tumor / Q000032:analysis*; D018394:CA-125 Antigen / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D006801:Humans; D037121:Plant Lectins / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D014909:Wheat Germ Agglutinins