Genetic control of neuronal activity in mice conditionally expressing TRPV1.
Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.
Arenkiel
Benjamin R
BR
Howard Hughes Medical Institute, Department of Neurobiology, Duke University Medical Center, Box 3209, Durham, North Carolina 27710, USA.
Klein
Marguerita E
ME
Davison
Ian G
IG
Katz
Lawrence C
LC
Ehlers
Michael D
MD
D000200:Action Potentials / Q000187:drug effects / Q000235:genetics; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D018390:Gene Targeting; D017930:Genes, Reporter; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D019426:Integrases / Q000096:biosynthesis; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D011995:Recombination, Genetic; D050916:TRPV Cation Channels / Q000235:genetics*; D014162:Transfection
D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases; D002211:Capsaicin