Synthesis and biological evaluation of novel C(6) modified baicalein derivatives as antioxidative agents.
Baicalein, one of the major flavones, was found to be responsible for the antioxidative activity of the traditional Chinese medicinal herb Huang-Qin ( Scutellaria baicalensis Georgi), which is widely used as an antioxidative, anti-inflammatory, and antitumor agent. The hydroxyl group of the A ring of the baicalein was alkylated at position 6 with terpenoids such as prenyl, geranyl, and farnesyl groups, and their free radical scavenging activities and glutathione (GSH) depletion capacities were examined. Their free radical scavenging activity was measured according to the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(*+)) scavenging method. Baicalein and newly synthesized baicalein derivatives were found to be good free radical scavengers. Flow cytometrical method was employed to measure the intracellular antioxidative activity and GSH depletion capacity of these derivatives in human acute monocytic leukemia cell line (THP-1). It was also found that baicalein and its derivatives could decrease the levels of exogenous cumene hydroperoxide and H2O2 in THP-1 cells. These compounds also could significantly inhibit the intracellular GSH depletion induced by cumene hydroperoxide in THP-1 cells. The production of cumene hydroperoxide-induced Bax, a pro-apoptotic related protein, could also be inhibited by baicalein and its derivatives. These results suggested that baicalein and its derivatives could be beneficial to human health.
Wu
Jin-Yi
JY
Graduate Institute of Biomedical and Biopharmaceutical Sciences, College of Life Sciences, National Chiayi University, 300 University Road, Chiayi 60004, Taiwan.
Chung
King-Thom
KT
Liu
Yi-Wen
YW
Lu
Fung-Jou
FJ
Tsai
Ruei-Shiun
RS
Chen
Chi-Hung
CH
Chen
Ching-Hsein
CH
D000478:Alkylation; D000975:Antioxidants / Q000138:chemical synthesis* / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology; D052160:Benzothiazoles; D045744:Cell Line, Tumor; D044950:Flavanones / Q000737:chemistry* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007948:Leukemia, Monocytic, Acute; D013451:Sulfonic Acids; D013729:Terpenes / Q000737:chemistry
D000975:Antioxidants; D001555:Benzene Derivatives; D052160:Benzothiazoles; D044950:Flavanones; D016166:Free Radical Scavengers; D013451:Sulfonic Acids; D013729:Terpenes; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C006680:baicalein; D005978:Glutathione; C007164:cumene hydroperoxide