Thalassospira tepidiphila sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from seawater.
A Gram-negative, mesophilic bacterial strain, designated 1-1B(T), which degrades polycyclic aromatic hydrocarbons, was isolated from petroleum-contaminated seawater during a bioremediation experiment. A 16S rRNA gene sequence analysis indicated that the isolate was affiliated with the genus Thalassospira in the Alphaproteobacteria; the sequence was found to be most similar to those of Thalassospira profundimaris WP0211(T) (99.8 %), Thalassospira xiamenensis M-5(T) (98.2 %) and Thalassospira lucentensis DSM 14000(T) (98.1 %). However, the levels of DNA-DNA relatedness between strain 1-1B(T) and these type strains were 50.7+/-17.2, 35.7+/-17.8 and 32.0+/-21.1 %, respectively. In addition, strain 1-1B(T) was found to be distinct from the other described species of the genus Thalassospira in terms of some taxonomically important traits, including DNA G+C content, optimum growth temperature, salinity tolerance, utilization of carbon sources and fatty acid composition. Furthermore, strain 1-1B(T) and T. profundimaris were also different with regard to motility and denitrification capacities. On the basis of physiological and DNA-DNA hybridization data, strain 1-1B(T) represents a novel species within the genus Thalassospira, for which the name Thalassospira tepidiphila sp. nov. is proposed. The type strain is 1-1B(T) (=JCM 14578(T) =DSM 18888(T)).
Kodama
Yumiko
Y
Laboratory of Applied Microbiology, Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Stiknowati
Lies Indah
LI
Ueki
Atsuko
A
Ueki
Katsuji
K
Watanabe
Kazuya
K
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000737:chemistry / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014876:Water Pollution
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S