title: Spectrofluorimetric study on the inclusion interaction between vitamin K3 with p-(p-sulfonated benzeneazo)calix[6]arene and determination of VK3.
abstract: The characteristics of host-guest complexation between p-(p-sulfonated benzeneazo) calix[6]arene (SBC6A) and vitamin K3 (VK3) were investigated by fluorescence spectrometry. A 1:1 stoichiometry for the complexation was established and was verified by Job's plot. An association constant of 4.95 x 10(3)L mol(-1) at 20 degrees C was calculated by applying a deduced equation. The interaction mechanism of the inclusion complex was discussed. It was found that the fluorescence of SBC6A could be remarkably quenched by an appropriate amount of VK3 especially when non-ionic surfactant Triton X-100 existed. According to the obtained results, a novel sensitive spectrofluorimetric method for the determination of VK3 based on supramolecular complex was developed with a linear range of 5.0 x 10(-7) -3.0 x 10(-5)mol L(-1) and a detection limit of 2.0 x 10(-7)mol L(-1). The proposed method was used to determine VK3 in commercial preparations with satisfactory results.
authors.affiliation: Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China. zy161299@mail.ahnu.edu.cn
authors.forename: Yunyou
authors.initials: Y
authors.lastname: Zhou
authors.forename: Hongwei
authors.initials: H
authors.lastname: Xu
authors.forename: Lian
authors.initials: L
authors.lastname: Wu
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Lu
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
mesh_terms: D002138:Calibration; D047250:Calixarenes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D024483:Vitamin K 3 / Q000032:analysis* / Q000737:chemistry*
chemical_list: C534156:4-(4-sulfonated benzeneazo)calix(6)arene; D007477:Ions; D010636:Phenols; C476540:calix(6)arene; D047250:Calixarenes; D024483:Vitamin K 3