The bone-derived collagen containing mineralized matrix for the loading of collagen-binding bone morphogenetic protein-2.
Bone tissue-derived biomaterials have often been applied for bone repair because of their similarity to human bone in structure and composition. When combined with growth factors, they could accelerate bone formation. Here, we explore a collagen containing mineralized bone-derived matrix (CCMBM) from bovine bone tissues, which not only maintains proper mechanical strength but also binds to the collagen-binding recombinant human collagen-binding bone morphogenetic protein-2 (CBD-BMP(2)). By analyzing its morphology and composition, we found that CCMBM was porous and mainly composed of calcium compounds. CCMBM could provide mechanical support for bone injury repair. It also showed good biocompatibility and proper degradation rate that would be helpful for bone regeneration. In addition, the intentionally preserved collagen allowed the specific binding of CBD-BMP(2) to CCMBM, and resulted in significantly increased osteogenesis in vivo. The results indicated that the combination of CCMBM with collagen-binding BMP(2) could be emerged into an effective medical device for bone repair.
Zhao
Yannan
Y
Center of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Chen
Bing
B
Lin
Hang
H
Sun
Wenjie
W
Zhao
Wenxue
W
Zhang
Jing
J
Dai
Jianwu
J
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001857:Bone Matrix / Q000378:metabolism* / Q000648:ultrastructure; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002113:Calcification, Physiologic; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D003094:Collagen