title: Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa.
abstract: Some members of the heat shock protein 70 (HSP70) family have important functions in organism development. Here, we identified an alfalfa (Medicago sativa L. cv. Algonquin) HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. This protein contains all conserved domains, motifs, and characteristic sequences of plant HSP70s. The expression of MsHSP70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia. These results suggested that MsHSP70-1 might play an important role in alfalfa nodule development.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Zhishui
authors.initials: Z
authors.lastname: He
authors.forename: Rong
authors.initials: R
authors.lastname: Xie
authors.forename: Yanzhang
authors.initials: Y
authors.lastname: Wang
authors.forename: Huasong
authors.initials: H
authors.lastname: Zou
authors.forename: Jiabi
authors.initials: J
authors.lastname: Zhu
authors.forename: Guanqiao
authors.initials: G
authors.lastname: Yu
mesh_terms: D003001:Cloning, Molecular; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000455:Medicago sativa / Q000235:genetics / Q000378:metabolism*; D018514:Plant Structures / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D018840:HSP70 Heat-Shock Proteins