title: [Structure of deletions detected in the genomes of clinical Mycobacterium tuberculosis strains].
abstract: Deletions were analyzed in the genomes of four Mycobacterium tuberculosis strains isolated from the sputum of patients living in the Central Region of Russia. The strains of the Beijing family were found to have deletions affecting 40 open reading frames (ORF) and to amount to 0.7% of the genome H37Rv. Genome deletions in a strain from the Haarlem family affected 20 ORF and accounted for 0.26% of the genome H37Rv. Six of the eight deletions were located at the site of preferred cloning on the insertion element IS6110. Prophage phiRv1-associated deletion sequence 149 is the only common to the strains of both families. The deletion ends were evenly distributed among the intergenic and coding chromosome regions with an insignificant preference of the latter. The authors revealed a new deletion in strain 1540 belonging to the Haarlem family and a two-component deletion in the region RvD2. The deletions detected in the genomes of Russian Beijing strains were typical of strains from South-East Asian countries.
authors.forename: A G
authors.initials: AG
authors.lastname: Bogun
authors.forename: V A
authors.initials: VA
authors.lastname: Anisimova
authors.forename: V N
authors.initials: VN
authors.lastname: Stepanshina
authors.forename: I G
authors.initials: IG
authors.lastname: Shemiakin
mesh_terms: D017931:DNA Primers / Q000235:genetics; D017353:Gene Deletion; D016680:Genome, Bacterial / Q000235:genetics*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D014397:Tuberculosis, Pulmonary / Q000235:genetics* / Q000382:microbiology*
chemical_list: D017931:DNA Primers