[Distribution of HIV/AIDS associated CCR2-64 I allele of 14 Chinese ethnic populations in South China].
The purpose of the work is to investigate the frequencies and polymorphisms of HIV-1 resistant CCR2-64 I alleles in 14 Chinese populations in South China. The blood samples include 791 unrelated healthy individuals. Whole blood samples were collected randomly and their genomic DNA were extracted with OMEGA Blood Kits. Allelic frequencies were identified by PCR-RFLP analysis. 636 individuals were wild-type homozygous, 104 individuals were CCR2-64 heterozygote and 51 individuals were CCR2-64 homozygous. Distributions of the CCR2-64 I alleles among the populations were in accordance with Hardy-Weinberg equilibrium. The average allele frequencies was 13.6%, and the frequency span of the alleles was 1.6%-30.3%. By statistical analysis, the frequency varied significantly in 14 populations (Plt;0.05). Among them, Zhuang ethnic population had the lowest CCR2-64 I allele, whereas the Lisu ethnic population in Yunnan province had the highest CCR2-64 I allele Conclusion: The frequency of CCR2-64 I allele of 12 ethnic populations were lower than that of Han. The frequency of CCR2-64 I allele between Southern and South-east group showed significant variations. The mutant alleles conferring genetic resistance to HIV-1 and AIDS progression needs to be further studied.
Qian
Yuan
Y
Chinese Academic of Medical Science, Institute of Medical Biology, Kunming 650118, China. yuanqian2x@hotmail.com
Lin
Ke-Qin
KQ
Shi
Lie
L
Shi
Li
L
Chu
Jia-You
JY
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D044466:Asian People; D002681:China; D005787:Gene Frequency; D015658:HIV Infections / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D054390:Receptors, CCR2 / Q000235:genetics*
C515334:CCR2 protein, human; D054390:Receptors, CCR2