In vivo single molecular imaging and sentinel node navigation by nanotechnology for molecular targeting drug-delivery systems and tailor-made medicine.
The recent advances in nanotechnology have a great potential to improve the prevention, diagnosis, and treatment of human diseases. Nanomaterials for medical applications are expected to grasp pharmacokinetics and the toxicity for application to medical treatment on the aspect of safety of the nanomaterials and nanodevices. We describe a generation of CdSe nanoparticles [quantum dots (QDs)] conjugated with monoclonal anti-HER2 antibody (Trastuzumab), for single molecular in vivo imaging of breast cancer cells. We established a high-resolution in vivo 3D microscopic system for a novel imaging method at the molecular level. The cancer cells expressing HER2 protein were visualized by the nanoparticles in vivo at subcellular resolution, suggesting future utilization of the system in medical applications to improve drug-delivery systems to target the primary and metastatic tumors for made-to-order treatment. We also describe sentinel node navigation using fluorescent nanoparticles for breast cancer surgery in experimental model, which have shown the potential to be an alternative to existing tracers in the detection of the sentinel node if we select the appropriate particle size and wavelength. Future innovation in cancer imaging by nanotechnology and novel measurement technology will provide great improvement, not only in the clinical field but also in basic medical science for the development of medicine.
Takeda
Motohiro
M
Department of Surgical Oncology, Graduate School of Medicine, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, 980-8574, Japan. motot@d1.dion.ne.jp
Tada
Hiroshi
H
Higuchi
Hideo
H
Kobayashi
Yoshio
Y
Kobayashi
Masaki
M
Sakurai
Yuu
Y
Ishida
Takanori
T
Ohuchi
Noriaki
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage*; D061067:Antibodies, Monoclonal, Humanized; D003952:Diagnostic Imaging / Q000379:methods*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D005260:Female; D005453:Fluorescence; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology; D021701:Sentinel Lymph Node Biopsy; D014018:Tissue Distribution; D000068878:Trastuzumab; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D004337:Drug Carriers; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab