title: Quantitative analysis of survivin mRNA expression in bladder transitional cell carcinomas.
abstract: We assessed the expression levels of survivin mRNA in bladder transitional cell carcinoma (TCC) to provide additional information regarding its malignant potential. The real-time PCR method was used to detect the survivin mRNA level for 21 bladder tumor specimens, and for urinary exfoliated cells from 12 newly diagnosed bladder tumor patients. All bladder tumor specimens and 7 of 12 voided urine specimens expressed survivin mRNA. In tumor specimens, high grade, high stage tumors had the tendency to express more survivin mRNA. Of 12 superficial bladder tumor patients who had transurethral resection of bladder tumor (TURB), 3 showed high survivin mRNA expression and intravesical recurrence after the surgery. However, for the patients who had total cystectomy due to invasive tumor, no relations were observed between the survivin mRNA expression level and development of local recurrence and/or distant metastasis. Our results suggested that the quantitative analysis of the survivin mRNA may indicate local malignant potential, which contribute to the possibility of an intravesical recurrence.
authors.affiliation: The Department of Urology, Tokyo Medical University Kasumigaura Hospital.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kitsukawa
authors.forename: Teiichiro
authors.initials: T
authors.lastname: Aoyagi
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Noda
authors.forename: Takaaki
authors.initials: T
authors.lastname: Ito
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Hosoda
authors.forename: Norihiko
authors.initials: N
authors.lastname: Otsuru
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000737:chemistry* / Q000601:surgery; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000235:genetics; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D000077022:Survivin; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000601:surgery
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D000077022:Survivin