title: Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients.
abstract: Blood and cerebrospinal fluid (CSF) of 30 Fisher grade 3 aneurysmal subarachnoid hemorrhage (ASAH) patients were analyzed for the presence of the phosphorylated axonal form of the major neurofilament subunit NF-H (pNF-H), a promising biomarker of axonal injury. Patient demographic data including development of vasospasm and outcome scores at 6 months after aneurysmal rupture (AR) were evaluated. Higher pNF-H blood levels in the first few days after AR were strongly predictive of a negative outcome. Blood pNF-H levels in most recovering patients showed a steady increase into the second week after AR, presumably reflecting axonal degeneration secondary to the original insult. Almost half of the patients studied showed sudden dramatic peaks of pNF-H protein release into CSF in the 3- to 14-day time period after AR, which must reflect profound, coordinated, and secondary loss of axons. Patients in whom vasospasm was detected had significantly more pNF-H in both blood and CSF compared with those in whom vasospasm was not detected. We conclude that the analysis of pNF-H levels in blood and CSF differentiates between patients with poor and favorable outcomes and also reveals several novel features of ASAH progression and recovery.
authors.affiliation: Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, Florida, USA.
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Lewis
authors.forename: Regina A
authors.initials: RA
authors.lastname: Wolper
authors.forename: Lynn
authors.initials: L
authors.lastname: Miralia
authors.forename: Cui
authors.initials: C
authors.lastname: Yang
authors.forename: Gerry
authors.initials: G
authors.lastname: Shaw
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017542:Aneurysm, Ruptured / Q000097:blood / Q000134:cerebrospinal fluid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000097:blood* / Q000134:cerebrospinal fluid*; D010766:Phosphorylation; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*; D020301:Vasospasm, Intracranial / Q000097:blood / Q000134:cerebrospinal fluid
chemical_list: D016900:Neurofilament Proteins; C051774:neurofilament protein H