Synthesis, chaperoning, and metabolism of proteins are regulated by NT-3/TrkC signaling in the medulloblastoma cell line DAOY.
The human medulloblastoma cell line DAOY was transfected with Tropomyosin receptor kinase (TrkC), a marker for good prognostic outcome. Following TrkC-activation by its ligand neurotrophin-3, protein extracts from DAOY cells were run on 2DE with subsequent MALDI-TOF-TOF analysis and quantification in order to detect downstream effectors. Protein levels of translational, splicing, processing, chaperone, protein handling, and metabolism machineries were shown to depend on neurotrophin-3-induced TrkC activation probably representing pharmacological targets.
Gruber-Olipitz
Mariella
M
Department of Pediatrics, Medical University of Vienna, Vienna, Austria.
Ströbel
Thomas
T
Chen
Wei-Qiang
WQ
Grotzer
Michael A
MA
Quehenberger
Franz
F
Slavc
Irene
I
Lubec
Gert
G
D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D002648:Child; D004789:Enzyme Activation; D006801:Humans; D008527:Medulloblastoma / Q000737:chemistry* / Q000175:diagnosis / Q000378:metabolism*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000378:metabolism*; D011379:Prognosis; D020812:Receptor, trkC / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020933:Neurotrophin 3; D020812:Receptor, trkC