title: HLA-DQ3 is a probable risk factor for CMV infection in high-risk kidney transplant patients.
abstract: BACKGROUND
  Cytomegalovirus (CMV) infection in transplant patients with special risk factors remains a major hazard. CMV-seronegative recipients with seropositive donors have the highest risk of developing acute CMV disease. We suggest that the HLA-type may influence the occurrence and the severity of primary CMV infection of these recipients and the measurement of the special HLA-types may be useful in the prediction of acute infection.
  
  
  METHODS
  Since 1999 1213 cadaver kidney transplantations have been performed in our clinic. 163 of 1213 recipients were CMV-seronegative (13%) and 129 of them received the kidney from seropositive donors. All 129 patients received CMV infection prophylaxis. Of 129 CMV-seronegative patients 49 developed acute CMV infection (38%) during the first posttransplant year. CMV infection was diagnosed by CMV antigenemia test and serologic measurements (ELISA). The particular HLA-genotypes of the recipients were studied before the transplantation. The occurrence and the severity of CMV infection was investigated in association with HLA-types.
  
  
  RESULTS
  We found different acute CMV infection distribution in the careers and non-careers of investigated HLA-types: HLA-A2, HLA-B12, HLA-Cw7, HLA-DR6 and HLA-DR11, but the differences were not significant in these HLA-types (P = 0.26, P = 0.37, P = 0.83, P = 0.07 and P = 0.37). While investigating HLA-DQ3, we found that of 68 DQ3-positive patients 32 (47%), of 61 DQ3-negative patients 17 (28%) had acute CMV infection and this difference was found to be significant. This result was confirmed by univariate and multivariate Cox Regression (P = 0.001) and the appropriate significance level was considered by Bonferroni correction.
  
  
  CONCLUSIONS
  HLA-DQ3 was found to be an independent predictor of CMV infection. Our data suggest that patients positive for HLA-DQ3 are more susceptible to CMV infection than a comparable group of patients negative for HLA-DQ3. This result was not due to rejection and/or treatment for rejection and was not influenced by induction therapy. Although we found more symptomatic infections among DQ3+ patients the difference was not significant (P = 0.19). Comparing the gender proportion among all 1213 kidney recipients and among CMV-seronegative recipients we found that the proportion of males is significantly higher among CMV-seronegative recipients (P < 0.001).
authors.affiliation: Transplantation and Surgical Clinic, Semmelweis University, Baross Street 23-25, Budapest 1082, Hungary. marisa1@freemail.hu
authors.forename: Marina
authors.initials: M
authors.lastname: Varga
authors.forename: Katalin
authors.initials: K
authors.lastname: Rajczy
authors.forename: Gábor
authors.initials: G
authors.lastname: Telkes
authors.forename: Márta
authors.initials: M
authors.lastname: Hídvégi
authors.forename: Antal
authors.initials: A
authors.lastname: Péter
authors.forename: Adám
authors.initials: A
authors.lastname: Remport
authors.forename: Márta
authors.initials: M
authors.lastname: Korbonits
authors.forename: János
authors.initials: J
authors.lastname: Fazakas
authors.forename: Eva
authors.initials: E
authors.lastname: Toronyi
authors.forename: Enikõ
authors.initials: E
authors.lastname: Sárváry
authors.forename: László
authors.initials: L
authors.lastname: Kóbori
authors.forename: Jenõ
authors.initials: J
authors.lastname: Járay
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003586:Cytomegalovirus Infections / Q000209:etiology* / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission; D005260:Female; D020022:Genetic Predisposition to Disease; D006084:Graft Rejection / Q000209:etiology / Q000276:immunology; D006683:HLA-DQ Antigens / Q000235:genetics / Q000378:metabolism*; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012307:Risk Factors; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D006683:HLA-DQ Antigens; C051569:HLA-DQ3 antigen