[Rapid determination of residues of 4 tetracyclines in meat by a microbiological screening, HPLC and LC/MS/MS].
A rapid and precise determination residues of 4 tetracyclines (TCs) (oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC) and doxycycline (DOXY)) in meat was developed by employing three analyses; a microbiological screening, HPLC and LC/MS/MS. TCs were extracted with pH 4.0 McIlvaine buffer containing 0.01 mol/L EDTA from a meat sample, and then purified using a mixed mode, reversed-phase and cation-exchange cartridge. The mean recoveries (n=5) of 0.2 microg/g OTC, TC and CTC, 0.05 microg/g DOXY spiked in meat samples were 76.6-99.0% (C.V. 1.6-5.4%). In 13 meat samples in which the microbiological screening indicated the presence of TCs, CTC (9 samples) and DOXY (4 samples) were identified with HPLC and LC/MS/MS.
Kanda
Maki
M
Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
Kusano
Tomoko
T
Osanai
Taka
T
Ushiyama
Keiko
K
Takeba
Kazue
K
Sakamoto
Miho
M
Hayashi
Hiroshi
H
Igusa
Kyoko
K
Ibe
Akihiro
A
Nagayama
Toshihiro
T
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002645:Chickens; D002751:Chlortetracycline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004318:Doxycycline / Q000032:analysis; D008460:Meat / Q000032:analysis*; D010118:Oxytetracycline / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis*
D013754:Tetracyclines; D004318:Doxycycline; D002751:Chlortetracycline; D010118:Oxytetracycline