MUC-1 mucin in normal human salivary glands detected by HMFG-1 and HMFG-2 monoclonal antibodies.
The aim of this study was to determine the immunoexpression of normal salivary gland cells using two human milk fat globule membrane protein antibodies (HMFG-1 and HMFG-2). Paraffin-embedded, 5 microm-thick slides from parotid and submandibular gland tissues were cut and immunostained with monoclonal antibodies HMFG-1 and HMFG-2 against MUC1 protein. HMFG antigens were found in secretory epithelial cells and ductal cells lining the striated and intercalated ducts in the intraductal secretion material, and sometimes in the myoepithelial cells. Our results suggest that HMFG-1 and HMFG-2 proteins may play a role in normal salivary gland function, mainly in the manufacturing and secretion of saliva.
Ponce-Bravo
Santa
S
Oral Pathology Laboratory. Facultad de Odontología, Universidad Nacional Autónoma de México, México.
Ledesma-Montes
Constantino
C
Morales-Sánchez
Israel
I
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D018396:Mucin-1 / Q000032:analysis*; D016612:Paraffin Embedding; D010306:Parotid Gland / Q000737:chemistry*; D013363:Submandibular Gland / Q000737:chemistry*
D000911:Antibodies, Monoclonal; C060192:MUC1 protein, human; D018396:Mucin-1; C469215:pemtumomab