title: Oligonucleotide-stabilized Ag nanocluster fluorophores.
abstract: Single-stranded oligonucleotides stabilize highly fluorescent Ag nanoclusters, with emission colors tunable via DNA sequence. We utilized DNA microarrays to optimize these scaffold sequences for creating nearly spectrally pure Ag nanocluster fluorophores that are highly photostable and exhibit great buffer stability. Five different nanocluster emitters have been created with tunable emission from the blue to the near-IR and excellent photophysical properties. Ensemble and single molecule fluorescence studies show that oligonucleotide encapsulated Ag nanoclusters exhibit significantly greater photostability and higher emission rates than commonly used cyanine dyes.
authors.affiliation: School of Chemistry and Biochemistry and Petit Institute for Biosciences and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
authors.forename: Chris I
authors.initials: CI
authors.lastname: Richards
authors.forename: Sungmoon
authors.initials: S
authors.lastname: Choi
authors.forename: Jung-Cheng
authors.initials: JC
authors.lastname: Hsiang
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Antoku
authors.forename: Tom
authors.initials: T
authors.lastname: Vosch
authors.forename: Angelo
authors.initials: A
authors.lastname: Bongiorno
authors.forename: Yih-Ling
authors.initials: YL
authors.lastname: Tzeng
authors.forename: Robert M
authors.initials: RM
authors.lastname: Dickson
mesh_terms: D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D012834:Silver; D004247:DNA