Cytokine responses in camels (Camelus bactrianus) vaccinated with Brucella abortus strain 19 vaccine.
In the present study, we determined the levels of cytokines produced by camel (Camelus bactrianus) peripheral blood mononuclear cells (PBMCs) in response to live attenuated Brucella abortus (B. abortus) S19 vaccine. Seven camels were vaccinated with commercial B. abortus S19 vaccine, and their cytokine responses were determined using a real-time PCR assay. Cytokine responses to B. abortus S19 were examined at 6 hr, 48 hr and 1, 2 and 3 weeks post-vaccination. Serological tests were performed to further confirm these immune responses. The results revealed that IFN-gamma and IL-6 were upregulated during the first week post-vaccination. Low level expressions of IL-1alpha, IL-1beta, TNFalpha and IL-10 and no expression of IL-2 and IL-4 were observed compared with the control camels. The findings showed that B. abortus stimulates cell-mediated immunity by directly activating camel Th1 cells to secrete IFN-gamma. This quantification of cytokine expression in camels is essential for understanding of Camelidae disease development and protective immune responses. This is the first report of in vivo camel cytokine quantification after vaccination.
Odbileg
Raadan
R
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Purevtseren
Byambaa
B
Gantsetseg
Dorj
D
Boldbaatar
Bazartseren
B
Buyannemekh
Tumurjav
T
Galmandakh
Zagd
Z
Erdenebaatar
Janchivdorj
J
Konnai
Satoru
S
Onuma
Misao
M
Ohashi
Kazuhiko
K
D000818:Animals; D002002:Brucella / Q000145:classification / Q000276:immunology*; D002004:Brucella Vaccine / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000662:veterinary*; D002162:Camelus / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D002004:Brucella Vaccine; D016207:Cytokines; D012333:RNA, Messenger