title: An efficient and convenient synthesis of deuterium-labelled seminolipid isotopomers and their ESI-MS characterization.
abstract: Seminolipids 1a and 1b and galactosylalkylacylglycerols 2a and 2b, labelled with deuterium on the alkyl or acyl chain, respectively, were obtained isotopically and chemically pure through a straightforward synthesis from protected glycidyl galactoside 3 in an overall 22% yield. The identity and purity of compounds was ascertained by NMR spectroscopy and ESI mass spectrometry analysis. These labelled compounds are important as internal standards for quantification of these lipids by mass spectrometry, and they could also be used in metabolic studies in in vitro and even in vivo systems. Extension of the procedure could provide a route for the preparation of isotopomers of other compounds of the same general class.
authors.affiliation: Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università di Milano, Via Saldini 50, 20133-Milano, Italy. franchini_laura@hotmail.com
authors.forename: Laura
authors.initials: L
authors.lastname: Franchini
authors.forename: Luigi
authors.initials: L
authors.lastname: Panza
authors.forename: Kessiri
authors.initials: K
authors.lastname: Kongmanas
authors.forename: Nongnuj
authors.initials: N
authors.lastname: Tanphaichitr
authors.forename: Kym F
authors.initials: KF
authors.lastname: Faull
authors.forename: Fiamma
authors.initials: F
authors.lastname: Ronchetti
mesh_terms: D000818:Animals; D002621:Chemistry / Q000379:methods; D003903:Deuterium / Q000737:chemistry*; D005260:Female; D006017:Glycolipids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008956:Models, Chemical; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013084:Sperm-Ovum Interactions; D013552:Swine; D013737:Testis / Q000378:metabolism
chemical_list: D006017:Glycolipids; D008055:Lipids; C011117:sulfoglycolipids; D003903:Deuterium