title: Is Alba an RNase P subunit?
abstract: It has been suggested that Alba, a well-established chromatin protein in Archaea, is also a subunit of the archaeal RNase P holoenzyme, based on the observation that the homolog of this protein in humans has been shown to be associated with RNase P activity. Using the same biochemical methods we used previously to show that four other proteins homologous to eukaryotic RNase P proteins are bona fide RNase P subunits in Archaea, we could not detect any association of the Alba homolog in Methanothermobacter thermoautotrophicus (Mth1483p) with the RNase P holoenzyme. In addition, the presence of Mth1483p did not enhance the activity of RNase P holoenzyme reconstituted from recombinant subunits. In conclusion, we find no evidence that Alba is an RNase P subunit.
authors.affiliation: Laboratory of Signal Transduction, NIEHS, Research Triangle Park, North Carolina, USA.
authors.forename: J Chris
authors.initials: JC
authors.lastname: Ellis
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Barnes
authors.forename: James W
authors.initials: JW
authors.lastname: Brown
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D004722:Endoribonucleases; D006801:Humans; D017013:Methanobacteriaceae / Q000201:enzymology; D021122:Protein Subunits / Q000737:chemistry* / Q000502:physiology; D016337:RNA, Catalytic; D043262:Ribonuclease P / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology
chemical_list: D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; D021122:Protein Subunits; D016337:RNA, Catalytic; C444360:Sso10b protein, Sulfolobus solfataricus; D004722:Endoribonucleases; D043262:Ribonuclease P