title: Whole-blood interferon-gamma assay for the diagnosis of tuberculosis infection in an unselected Greek population.
abstract: OBJECTIVE
  Although QuantiFERON-TB Gold (QFT-G) has been approved for the diagnosis of latent tuberculosis infection (LTBI), there are limited data regarding its performance in routine clinical practice. The aim of this study was to compare QFT-G 'In Tube' results, based on stimulation with Mycobacterium tuberculosis-specific antigens, with tuberculin skin test (TST) results in an unselected hospital-based Greek population.
  
  
  METHODS
  This was a prospective study of inpatients and outpatients in the University Hospital of Larissa, Greece. Demographic, clinical and microbiological data were collected and correlated to the QFT-G 'In Tube' results. Agreement was tested using the Q-test; kappa coefficient and confounding factors were adjusted for by univariate and multivariable logistic regression.
  
  
  RESULTS
  Among the 191 patients with valid results for both tests, the concordance between the TST and the QFT-G was 68.5%, with a kappa value of 0.38 (95% CI 0.2-0.5). In 27 (14.1%) patients with active tuberculosis, QFT-G 'In Tube' yielded 85.1% positive results compared with 74% for the TST (P = 0.45).
  
  
  CONCLUSIONS
  Overall agreement between the QFT-G 'In Tube' assay and the TST in this unselected hospital population was low, mainly due to discordant TST-positive/QFT-negative results.
authors.affiliation: Department of Respiratory Medicine, Medical School, University of Larissa, Larissa, Greece. igerogianni@yahoo.gr
authors.forename: Irini
authors.initials: I
authors.lastname: Gerogianni
authors.forename: Maria
authors.initials: M
authors.lastname: Papala
authors.forename: Dimitra
authors.initials: D
authors.lastname: Klapsa
authors.forename: Elias
authors.initials: E
authors.lastname: Zinzaras
authors.forename: Efi
authors.initials: E
authors.lastname: Petinaki
authors.forename: K I
authors.initials: KI
authors.lastname: Gourgoulianis
mesh_terms: D000328:Adult; D000368:Aged; D001500:BCG Vaccine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006115:Greece; D006801:Humans; D016867:Immunocompromised Host; D007371:Interferon-gamma / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D015203:Reproducibility of Results; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000175:diagnosis*
chemical_list: D001500:BCG Vaccine; D007371:Interferon-gamma