title: Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes.
abstract: A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.
authors.affiliation: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, 060-8628, Japan. takagi-m@eng.hokudai.ac.jp
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Takagi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kitabayashi
authors.forename: Satoru
authors.initials: S
authors.lastname: Koizumi
authors.forename: Haruka
authors.initials: H
authors.lastname: Hirose
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kondo
authors.forename: Masashi
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Kosei
authors.initials: K
authors.lastname: Ueno
authors.forename: Misawa
authors.initials: M
authors.lastname: Hiroaki
authors.forename: Yoichiroh
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masuhara
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Wakitani
mesh_terms: D053674:Aggrecans / Q000096:biosynthesis*; D015703:Antigens, CD / Q000096:biosynthesis; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology*; D048430:Cell Shape / Q000502:physiology*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D005326:Fetal Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D018800:Thy-1 Antigens / Q000096:biosynthesis; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D053674:Aggrecans; D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D012333:RNA, Messenger; D018800:Thy-1 Antigens; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule