High-density DNA alignment on an Au(111) surface starting from folded DNA.
High-density uniform DNA alignment on a metal substrate is essential for creating sensitive DNA devices. We develop a self-sensing DNA alignment process starting from folded DNA to achieve high-density, uniform DNA alignment on an Au(111) surface. We demonstrate that folded DNA plays a critical role in avoiding DNA aggregation and distributing the DNA uniformly on an Au(111) surface at the greatest density and quality ever attained. We also verify that the distributed, folded DNA can be stimulated to align only when the appropriate buffer flow is applied. This selective self-sensing DNA alignment on an Au surface will be a key technology for creating dynamic DNA sensors and switches.
Matsuura
Hiroshi
H
Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan. hmatsu32@sanken.osaka-u.ac.jp
Hirai
Ayako
A
Yamada
Fumihiko
F
Matsumoto
Takuya
T
Kawai
Tomoji
T
D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D013499:Surface Properties
D006046:Gold; D004247:DNA