[Anticoagulant activity of low-molecular-weight heparins obtained using a hydrolase complex].
The anticoagulant activity of low-molecular weight heparins (LMWH-PC) with average distribution of molecular weights within 3.4-5.8 kD was investigated. The samples of LMWH-PC were obtained from unfractionated heparin using immobilized enzyme complex of protease C. The LMWH-PC derivatives inhibited the activity of blood coagulation factors IIa (thrombin) and Xa. The LMWH-PC derivatives had an anti-factor-Xa activity up to 131-208 IU/mg and anti-factor-IIa activity up to 81-175 IU/mg. All LMWH-PC derivatives form complexes with protamine sulfate during electrophoresis in agarose gel. The anticoagulant activity of rabbit plasma exhibits a doze-dependent increase upon the intravenous or subcutaneous injection of LMWH-PC with a molecular weight of 5.4 kD.
Drozd
N N
NN
Tolstenkov
A S
AS
Bannikova
G E
GE
Miftakhova
N T
NT
Lapikova
E S
ES
Makarov
V A
VA
Varlamov
V P
VP
D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004800:Enzymes, Immobilized / Q000737:chemistry; D065427:Factor Xa Inhibitors; D006493:Heparin / Q000737:chemistry; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011479:Protamines / Q000737:chemistry; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits; D012697:Serine Endopeptidases / Q000737:chemistry; D013917:Thrombin / Q000037:antagonists & inhibitors*
D000925:Anticoagulants; D004800:Enzymes, Immobilized; D065427:Factor Xa Inhibitors; D006495:Heparin, Low-Molecular-Weight; D011479:Protamines; D011480:Protease Inhibitors; D006493:Heparin; D012697:Serine Endopeptidases; C088701:protease C; D013917:Thrombin