title: Calcitonin gene-related peptide release from intact isolated dorsal root and trigeminal ganglia.
abstract: Neuropeptides like calcitonin gene-related peptide (CGRP) and substance P are found in significant proportions of primary afferent neurons. Release of these neuropeptides as well as prostaglandin E(2) is an approved index for the activation of these primary afferents. Previous studies have used cultures of enzyme-treated and mechanically dissociated primary afferent neurons, fresh tissue slices or cubes. In the present study we demonstrate CGRP and prostaglandin E(2) release from intact isolated dorsal root and trigeminal ganglia. Stimulation with noxious heat, low pH, inflammatory mediators and high potassium concentration increased CGRP release. In conclusion, neuropeptide release from intact isolated ganglia is a reliable method to study the responsiveness of sensory neurons in situ in comparison with neuronal cell cultures.
authors.affiliation: Institute of Physiology and Pathophysiology, University of Erlangen-Nuernberg, Erlangen, Germany.
authors.forename: Mirjam
authors.initials: M
authors.lastname: Eberhardt
authors.forename: Tal
authors.initials: T
authors.lastname: Hoffmann
authors.forename: Susanne K
authors.initials: SK
authors.lastname: Sauer
authors.forename: Karl
authors.initials: K
authors.lastname: Messlinger
authors.forename: Peter W
authors.initials: PW
authors.lastname: Reeh
authors.forename: Michael J M
authors.initials: MJ
authors.lastname: Fischer
mesh_terms: D000143:Acids / Q000494:pharmacology; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D018836:Inflammation Mediators / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical; D013373:Substance P / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000143:Acids; D018836:Inflammation Mediators; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D011188:Potassium