title: Genotoxicity of visible light (400-800 nm) and photoprotection assessment of ectoin, L-ergothioneine and mannitol and four sunscreens.
abstract: This study was designed to determine the genotoxic effects of visible (400-800nm) and ultraviolet A (UVA)/visible (315-800nm) lights on human keratinocytes and CHO cells. The alkaline comet assay was used to quantify DNA-damage. In addition, photo-dependent cytogenetic lesions were assessed in CHO cells by the micronucleus test. Three protective compounds [ectoin, l-ergothioneine (ERT) and mannitol] were tested with the comet assay for their effectiveness to reduce DNA single-strand breaks (SSB). Finally, the genomic photoprotections of two broad-band sunscreens and their tinted analogues were assessed by the comet assay. The WST-1 cytotoxicity assay revealed a decrease of the keratinocyte viability of 30% and 13% for the highest UVA/visible and visible irradiations (15 and 13.8J/cm(2), respectively). Visible as well as UVA/visible lights induced DNA SSB and micronuclei, in a dose-dependent manner. The level of DNA breakage induced by visible light was 50% of the one generated by UVA/visible irradiation. However, UVA radiations were 10 times more effective than visible radiations to produce SSB. The DNA lesions induced by visible and UVA/visible lights were reduced after a 1-h preincubation period with the three tested compounds. The maximal protective effects were 92.7%, 97.9% and 52.0% for ectoin (0.1mM), ERT (0.5mM) and mannitol (1.5mM), respectively, against visible light and 68.9%, 59.8% and 62.7% for ectoin (0.1mM), ERT (0.5mM) and mannitol (1.5mM), respectively, against UVA/visible light. Thus, visible light was genotoxic on human keratinocytes and CHO cells through oxidative stress mechanisms similar to the ones induced by UVA radiations. The four tested sunscreens efficiently prevented DNA lesions that were induced by both visible and UVA/visible irradiations. The tinted sunscreens were slightly more effective that their colorless analogues. There is a need to complement sunscreen formulations with additional molecules to obtain a complete internal and external photoprotection against both UVA and visible lights.
authors.affiliation: Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale (EA 1784), Facultés de Médecine et Pharmacie, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Botta
authors.forename: Carole
authors.initials: C
authors.lastname: Di Giorgio
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Sabatier
authors.forename: Michel
authors.initials: M
authors.lastname: De Méo
mesh_terms: D000599:Amino Acids, Diamino / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016466:CHO Cells; D002478:Cells, Cultured; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects; D004880:Ergothioneine / Q000494:pharmacology; D006801:Humans; D007231:Infant, Newborn; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D008027:Light / Q000009:adverse effects*; D008353:Mannitol / Q000494:pharmacology; D015162:Micronucleus Tests; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013473:Sunscreening Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000599:Amino Acids, Diamino; D000975:Antioxidants; D011837:Radiation-Protective Agents; D013473:Sunscreening Agents; D008353:Mannitol; C045628:ectoine; D004880:Ergothioneine