Intranasal delivery of DNA encoding antigens of Mycobacterium tuberculosis by non-pathogenic invasive Escherichia coli.
Naturally invasive bacteria have been successfully used for mucosal delivery of DNA vaccines against bacterial, viral and tumour antigens. Recently, an alternative delivery system based on a genetically modified mutant of the non-pathogenic commensal bacterium Escherichia coli, was developed and successfully used to deliver therapeutic genes and immunogenic proteins to epithelial cells in vivo. In this work, we used these recombinant invasive bacteria to deliver DNA vaccines against two Mycobacterium tuberculosis proteins (FbpA, and HtpX) following intranasal administration. Both DNA vaccines were able to induce an antigen-specific T-cell response. Moreover, mice immunized with the recombinant bacteria carrying the DNA vaccine encoding HtpX, were significatively protected from challenge with M. tuberculosis.
Brun
Paola
P
Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, 35121 Padua, Italy.
Zumbo
Antonella
A
Castagliuolo
Ignazio
I
Delogu
Giovanni
G
Manfrin
Filippo
F
Sali
Michela
M
Fadda
Giovanni
G
Grillot-Courvalin
Catherine
C
Palù
Giorgio
G
Manganelli
Riccardo
R
D000217:Acyltransferases / Q000096:biosynthesis / Q000235:genetics; D000281:Administration, Intranasal; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D007115:Immunization Schedule; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology* / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000008:administration & dosage*; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D006360:Heat-Shock Proteins; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis