Study of the potential spermatogonial stem cell compartment in dogfish testis, Scyliorhinus canicula L.
In the lesser-spotted dogfish (Scyliorhinus canicula), spermatogenesis takes place within spermatocysts made up of Sertoli cells associated with stage-synchronized germ cells. As shown in testicular cross sections, cysts radiate in maturational order from the germinative area, where they are formed, to the opposite margin of the testis, where spermiation occurs. In the germinative zone, which is located in a specific area between the tunica albuginea of the testis and the dorsal testicular vessel, individual large spermatogonia are surrounded by elongated somatic cells. The aim of this study has been to define whether these spermatogonia share characteristics with spermatogonial stem cells described in vertebrate and non-vertebrate species. We have studied their ultrastructure and their mitotic activity by 5'-bromo-2'-deoxyuridine (BrdU) incorporation and proliferating cell nuclear antigen (PCNA) immunodetection. Additionally, immunodetection of c-Kit receptor, a marker of differentiating spermatogonia in rodents, and of alpha- and beta-spectrins, as constituents of the spectrosome and the fusome, has been performed. Ultrastructurally, nuclei of stage I spermatogonia present the same mottled aspect in dogfish as undifferentiated spermatogonia nuclei in rodents. Moreover, intercellular bridges are not observed in dogfish spermatogonia, although they are present in stage II spermatogonia. BrdU and PCNA immunodetection underlines their low mitotic activity. The presence of a spectrosome-like structure, a cytological marker of the germline stem cells in Drosophila, has been observed. Our results constitute the first step in the study of spermatogonial stem cells and their niche in the dogfish.
Loppion
Geraldine
G
UMR M 100 Ifremer, Physiologie et Ecophysiologie des Mollusques Marins, IFR 146 ICORE, Université de Caen Basse-Normandie, Caen, France.
Crespel
Amélie
A
Martinez
Anne-Sophie
AS
Auvray
Pierrïck
P
Sourdaine
Pascal
P
D000818:Animals; D004284:Dogfish / Q000502:physiology; D015151:Immunoblotting; D008297:Male; D008938:Mitosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D013049:Spectrin / Q000032:analysis; D013091:Spermatogenesis; D013093:Spermatogonia / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure*; D013234:Stem Cells / Q000166:cytology; D013737:Testis / Q000166:cytology* / Q000502:physiology
D018809:Proliferating Cell Nuclear Antigen; D013049:Spectrin; D019009:Proto-Oncogene Proteins c-kit