title: Dematin and adducin provide a novel link between the spectrin cytoskeleton and human erythrocyte membrane by directly interacting with glucose transporter-1.
abstract: Dematin and adducin are actin-binding proteins located at the spectrin-actin junctions, also called the junctional complex, in the erythrocyte membrane. Here we propose a new model whereby dematin and adducin link the junctional complex to human erythrocyte plasma membrane. Using a combination of surface labeling, immunoprecipitation, and vesicle proteomics approaches, we have identified glucose transporter-1 as the receptor for dematin and adducin in the human erythrocyte membrane. This finding is the first description of a transmembrane protein that binds to dematin and adducin, thus providing a rationale for the attachment of the junctional complex to the lipid bilayer. Because homologues of dematin, adducin, and glucose transporter-1 exist in many non-erythroid cells, we propose that a conserved mechanism may exist that couples sugar and other related transporters to the actin cytoskeleton.
authors.affiliation: Department of Pharmacology, University of Illinois Cancer Center, University of Illinois College of Medicine, Chicago, IL 60612, USA.
authors.forename: Anwar A
authors.initials: AA
authors.lastname: Khan
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Hanada
authors.forename: Morvarid
authors.initials: M
authors.lastname: Mohseni
authors.forename: Jong-Jin
authors.initials: JJ
authors.lastname: Jeong
authors.forename: Lixiao
authors.initials: L
authors.lastname: Zeng
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gaetani
authors.forename: Donghai
authors.initials: D
authors.lastname: Li
authors.forename: Brent C
authors.initials: BC
authors.lastname: Reed
authors.forename: David W
authors.initials: DW
authors.lastname: Speicher
authors.forename: Athar H
authors.initials: AH
authors.lastname: Chishti
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011485:Protein Binding; D040901:Proteomics; D000099291:Adducins
chemical_list: D001798:Blood Proteins; D002148:Calmodulin-Binding Proteins; D051272:Glucose Transporter Type 1; D000099291:Adducins