SLP-2 interacts with prohibitins in the mitochondrial inner membrane and contributes to their stability.
Stomatin is a member of a large family of proteins including prohibitins, HflK/C, flotillins, mechanoreceptors and plant defense proteins, that are thought to play a role in protein turnover. Using different proteomic approaches, we and others have identified SLP-2, a member of the stomatin gene family, as a component of the mitochondria. In this study, we show that SLP-2 is strongly associated with the mitochondrial inner membrane and that it interacts with prohibitins. Depleting HeLa cells of SLP-2 lead to increased proteolysis of prohibitins and of subunits of the respiratory chain complexes I and IV. Further supporting the role of SLP-2 in regulating the stability of specific mitochondrial proteins, we found that SLP-2 is up-regulated under conditions of mitochondrial stress leading to increased protein turnover. These data indicate that SLP-2 plays a role in regulating the stability of mitochondrial proteins including prohibitins and subunits of respiratory chain complexes.
Da Cruz
Sandrine
S
Department of Cell Biology, University of Geneva, Geneva, Switzerland.
Parone
Philippe A
PA
Gonzalo
Philippe
P
Bienvenut
Willy V
WV
Tondera
Daniel
D
Jourdain
Alexis
A
Quadroni
Manfredo
M
Martinou
Jean-Claude
JC
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D042967:Electron Transport Complex I / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D000091402:Prohibitins; D012097:Repressor Proteins / Q000378:metabolism*
D001798:Blood Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000091402:Prohibitins; D012097:Repressor Proteins; C406287:STOML2 protein, human; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I