title: Immobilization of penicillin G acylase on poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-grafted magnetic microspheres.
abstract: Poly[(glycidyl methacrylate)-co-(glycerol monomethacrylate)]-grafted magnetic microspheres were prepared by graft random copolymerization via ATRP from polymer microspheres with dispersed Fe(3)O(4) nanoparticles. Penicillin G acylase (PGA) was immobilized onto the polymer brush-grafted magnetic microspheres. The immobilized PGA prepared with initial glycidyl methacrylate/glycerol monomethacrylate ratios of 40/60 to 60/40 possessed higher catalytic activity than that prepared with higher proportions of glycidyl methacrylate in the initial monomer mixture. The immobilized PGA showed high thermal stability and enhanced tolerability to the pH variance.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Huang
authors.forename: Xiutao
authors.initials: X
authors.lastname: Li
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ruiying
authors.initials: R
authors.lastname: Zhao
authors.forename: Xicai
authors.initials: X
authors.lastname: Gao
authors.forename: Husheng
authors.initials: H
authors.lastname: Yan
mesh_terms: D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D004852:Epoxy Compounds / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009994:Osmolar Concentration; D010399:Penicillin Amidase / Q000737:chemistry* / Q000378:metabolism; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D005290:Ferric Compounds; D005296:Ferrous Compounds; D008689:Methacrylates; D011109:Polymethacrylic Acids; C000499:ferric oxide; C042535:polyglycidyl methacrylate; D010399:Penicillin Amidase; C034236:ferrous oxide; C007870:glycidyl methacrylate