title: The protective efficacy against Schistosoma japonicum infection by immunization with DNA vaccine and levamisole as adjuvant in mice.
abstract: Levamisole (LMS) as an adjuvant enhances cell-mediated immunity in DNA vaccination; we investigated the efficacy and liver immunopathology alleviation of a DNA vaccine, VR1012-SjGST-32, in a LMS formulation in the murine challenge model. Compared to controls, the VR1012-SjGST-32 plus LMS can reduce worm and egg burdens, as well as, immunopathological complications associated chronic inflammation significantly in liver, which were apparently associated with Th1-type response. Together, these results suggest that the LMS as a potential Schistosome DNA vaccine adjuvant can enhance both worm killing and disease prevention, which is possibly mediated through the induction of a strong Th1-dominant environment in immunized mice.
authors.affiliation: State Key Laboratory for Agro-Biotechnology, College of Biological Science, China Agricultural University, 2 Yuanmingyuan Xi Road, Beijing 100094, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Huali
authors.initials: H
authors.lastname: Jin
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Du
authors.forename: Chun
authors.initials: C
authors.lastname: Cai
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Gan
authors.initials: G
authors.lastname: Zhao
authors.forename: Baowei
authors.initials: B
authors.lastname: Su
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Hu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Luo
authors.forename: Ruiping
authors.initials: R
authors.lastname: She
authors.forename: Xinsong
authors.initials: X
authors.lastname: Luo
authors.forename: Xianfang
authors.initials: X
authors.lastname: Zeng
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Yi
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology; D049109:Cell Proliferation; D005260:Female; D005434:Flow Cytometry; D007978:Levamisole / Q000008:administration & dosage*; D008099:Liver / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000517:prevention & control*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D019444:Vaccines, DNA; D007978:Levamisole