title: [Detection the coinfection of Hantavirus and Orientia tsutsugamushi in primary cultured mite cells].
abstract: OBJECTIVE
  To investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).
  
  
  METHODS
  Collecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear. In situ RT-PCR and PCR were used to detect and locate HV RNA and Ot DNA in the primary cultured cells.
  
  
  RESULTS
  Positive signals of HV RNA and Ot DNA distributed mostly in epithelial cells of digestive system and ovary cells of larva and nymph. The positive rate increased by the generation of passages.
  
  
  CONCLUSIONS
  Coinfection of HV and Ot did exist in wild Leptotrombidium scutellare.
authors.affiliation: Institute of Military Medicine, Nanjing Command, Nanjing 210002, China.
authors.forename: Xiao-zhao
authors.initials: XZ
authors.lastname: Deng
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Chun-mei
authors.initials: CM
authors.lastname: Jiang
authors.forename: Zhong-can
authors.initials: ZC
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Kong
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Diao
authors.forename: Jun-ying
authors.initials: JY
authors.lastname: Qian
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guang-wen
authors.initials: GW
authors.lastname: Cao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006476:Orthohantavirus / Q000302:isolation & purification*; D051379:Mice; D008925:Mites / Q000382:microbiology* / Q000821:virology*; D012285:Orientia tsutsugamushi / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D012367:RNA, Viral