title: Middle cerebral artery occlusion in the rabbit using selective angiography: application for assessment of thrombolysis.
abstract: OBJECTIVE
  An animal model of selective middle cerebral artery (MCA) occlusion is needed for evaluation of intra-arterial (IA) delivery of thrombolytic agents. We describe a technique for MCA thrombo-occlusion in the rabbit with real-time angiographic documentation of occlusion and thrombolytic recanalization.
  
  
  METHODS
  After femoral artery cutdown, a microcatheter was advanced from the internal carotid artery to the MCA. MCA occlusion was achieved by IA thrombin and reperfusion by IA plasmin.
  
  
  RESULTS
  The terminal internal carotid artery was successfully catheterized in 12 of 13 animals. Stable (2-hour) MCA occlusion was induced and verified angiographically in all 12 animals; 2 animals also had distal internal carotid artery thrombus. Recanalization was achieved rapidly after IA plasmin in 3 of 3 animals.
  
  
  CONCLUSIONS
  We describe a new animal model of selective MCA occlusion documented by real-time angiography and used to demonstrate recanalization with IA plasmin.
authors.forename: Reza
authors.initials: R
authors.lastname: Jahan
authors.forename: Daphne
authors.initials: D
authors.lastname: Stewart
authors.forename: Harry V
authors.initials: HV
authors.lastname: Vinters
authors.forename: William
authors.initials: W
authors.lastname: Yong
authors.forename: Fernando
authors.initials: F
authors.lastname: Vinuela
authors.forename: Pete
authors.initials: P
authors.lastname: Vandeberg
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Marder
mesh_terms: D000818:Animals; D002533:Cerebral Angiography / Q000379:methods*; D004195:Disease Models, Animal; D005341:Fibrinolysin / Q000494:pharmacology / Q000627:therapeutic use; D005343:Fibrinolytic Agents / Q000494:pharmacology / Q000627:therapeutic use; D020244:Infarction, Middle Cerebral Artery / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D020766:Intracranial Embolism / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D020768:Middle Cerebral Artery / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D011237:Predictive Value of Tests; D011817:Rabbits; D013917:Thrombin; D015912:Thrombolytic Therapy / Q000379:methods*; D016896:Treatment Outcome; D014656:Vascular Surgical Procedures
chemical_list: D005343:Fibrinolytic Agents; D013917:Thrombin; D005341:Fibrinolysin