High-resolution high-speed panoramic cardiac imaging system.
A panoramic cardiac imaging system consisting of three high-speed CCD cameras has been developed to image the surface electrophysiology of a rabbit heart via fluorescence imaging using a voltage-sensitive fluorescent dye. A robust, unique mechanical system was designed to accommodate the three cameras and to adapt to the requirements of future experiments. A unified computer interface was created for this application - a single workstation controls all three CCD cameras, illumination, stimulation, and a stepping motor that rotates the heart. The geometric reconstruction algorithms were adapted from a previous cardiac imaging system. We demonstrate the system by imaging a polymorphic cardiac tachycardia.
Evertson
Dale W
DW
Department of Mechanical Engineering, Vanderbilt University, Nashville, TN 37235, USA.
Holcomb
Mark R
MR
Eames
Matthew C
MC
Bray
Mark-Anthony
MA
Sidorov
Veniamin Y
VY
Xu
Junkai
J
Wingard
Holley
H
Dobrovolny
Hana M
HM
Woods
Marcella C
MC
Gauthier
Daniel J
DJ
Wikswo
John P
JP
D000465:Algorithms; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000175:diagnosis* / Q000503:physiopathology*; D018780:Body Surface Potential Mapping / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006329:Heart Conduction System / Q000503:physiopathology*; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D011817:Rabbits