title: Neurite outgrowth and in vivo sensory innervation mediated by a Ca(V)2.2-laminin beta 2 stop signal.
abstract: Axons and dendrites of developing neurons establish distributed innervation patterns enabling precise discrimination in sensory systems. We describe the role of the extracellular matrix molecule, laminin beta2, interacting with the Ca(V)2.2 calcium channel in establishing appropriate sensory innervation. In vivo, Ca(V)2.2 is expressed on the growth cones of Xenopus laevis sensory neurites and laminin beta2 is expressed in the skin. Culturing neurons on a laminin beta2 substrate inhibits neurite outgrowth in a specific and calcium-dependent manner. Blocking signaling between laminin beta2 and Ca(V)2.2 leads to increased numbers of sensory terminals in vivo. These findings suggest that interactions between extracellular matrix molecules and calcium channels regulate connectivity in the developing nervous system.
authors.affiliation: Neurobiology Section, University of California, San Diego, La Jolla, California 92093-0357, USA.
authors.forename: Sharon B
authors.initials: SB
authors.lastname: Sann
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishimune
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Sanes
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Spitzer
mesh_terms: D000818:Animals; D020864:Calcium Channels, N-Type / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018388:Codon, Terminator / Q000235:genetics / Q000502:physiology*; D005260:Female; D006131:Growth Inhibitors / Q000502:physiology*; D007797:Laminin / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D009433:Neural Inhibition / Q000235:genetics; D016501:Neurites / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology*; D014176:Protein Biosynthesis / Q000235:genetics / Q000502:physiology; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics* / Q000502:physiology; D029867:Xenopus Proteins / Q000502:physiology; D014982:Xenopus laevis
chemical_list: C527798:CACNA1B protein, Xenopus; D020864:Calcium Channels, N-Type; D018388:Codon, Terminator; D006131:Growth Inhibitors; D007797:Laminin; D021122:Protein Subunits; D029867:Xenopus Proteins; C058783:laminin beta2