title: Therapeutic target metabolism observed using hyperpolarized 15N choline.
abstract: Choline is a precursor of cellular phospholipid metabolism that provides Magnetic Resonance (MR) and Positron Emission Tomography (PET) biomarkers for cancer detection and response assessment. Employing Dynamic Nuclear Polarization we show that the MR signal of 15N in choline can be enhanced by at least 4 orders of magnitude with a relaxation time of ca. 4 min, providing a method to observe the action of choline kinase, an important target for novel cancer therapeutics.
authors.affiliation: Cancer Research UK Clinical Magnetic Resonance Research Group, Institute of Cancer Research, Royal Marsden NHS Foundation Trust, Downs Road Sutton, Surrey, SM2 5PT, United Kingdom.
authors.forename: Cristina
authors.initials: C
authors.lastname: Gabellieri
authors.forename: Steven
authors.initials: S
authors.lastname: Reynolds
authors.forename: Arnon
authors.initials: A
authors.lastname: Lavie
authors.forename: Geoffrey S
authors.initials: GS
authors.lastname: Payne
authors.forename: Martin O
authors.initials: MO
authors.lastname: Leach
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Eykyn
mesh_terms: D002794:Choline / Q000737:chemistry* / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010767:Phosphorylcholine / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009587:Nitrogen Isotopes; D010767:Phosphorylcholine; D002797:Choline Kinase; D002794:Choline