[Effects of AST and ASI on metabolism of free radical in senescent rats treated by HC].
OBJECTIVE
To study the effects of Astragaloside (AST) and Astragalus Saponin I (ASI) on metabolism of free radical and immune function in senescent rats treated by HC.


METHODS
Hydrocorisone (HC) was used to estabilsh the aging model in rats. The content of molondialdehyde (MDA), glutathoine (GSH) and oxidized glutathoine (GSSG) in liver and brain was detected according to kit. The activity of Mn superoride dismulase (Mn-SOD) and catalase (CAT) was also surveyed by kit. Concanavalin (ConA) was used to detect the proliferation and interleukin-2 (IL-2) production of splenocytes.


RESULTS
Compared with HC control, AST and ASI could decrease the content of MDA, GSH and GSSG in liver and brain, increase the activity of Mn-SOD and CAT, and promote the proliferation and interleukin-2 (IL-2) activity of splenocytes.


CONCLUSIONS
AST and ASI could delay the aging effect in rats treated by HC, and its mechanism maybe the antioxidant and regulating immunity.
Li
Wei-Zu
WZ
Dept of pharmacology, Anhui Medical University, Hefei 230032, China.
Li
Wei-Ping
WP
Yin
Yan-Yan
YY
D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D027885:Astragalus propinquus / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002540:Cerebral Cortex / Q000378:metabolism; D005260:Female; D005609:Free Radicals / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006854:Hydrocortisone / Q000633:toxicity; D007376:Interleukin-2 / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013482:Superoxide Dismutase / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
D005609:Free Radicals; D007376:Interleukin-2; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D019803:Glutathione Disulfide; D006854:Hydrocortisone