[Effect of anisomycin on allogeneic skin transplantation in mice].
OBJECTIVE
To investigate the effect of anisomycin on the reactivity of lymphocytes and the rejection in the skin transplantation in mice.


METHODS
The reactivity of T cells in unilateral or bilateral mixed lymphocyte reaction was detected by MTT assay. The skins from C57BL/6 mice were transplanted to BALB/c mice and the survival period of the grafts was assessed. The recipients were divided into three groups (physiological saline group, cyclosporin A group and anisomycin group). They were injected with physiological saline (0.1 mL), cyclosporin A (20 mg/kg) and anisomycin (12.5 mg/kg) separately once a day for 3 days before the operation and 7 days after the operation.


RESULTS
With the optimal dose of 10.0 microg/L, anisomycin inhibited the reactivity of lymphocyte cells in both unilateral and bilateral mixed lymphocyte reactions. The survival period of the grafts from anisomycin group was longer than that from cyclosporin A group and physiological saline group.


CONCLUSIONS
Anisomycin can inhibit the reaction of lymphocytes and prolong the survival period of the grafts in skin transplantation effectively. It could be used as a new way for transplantation rejection treatment.
Chen
Di
D
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China.
Xing
Fei-yue
FY
DI
Jing-fang
JF
Chen
Ling
L
D000818:Animals; D000841:Anisomycin / Q000627:therapeutic use*; D006084:Graft Rejection / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation / Q000379:methods*; D014184:Transplantation, Homologous / Q000379:methods*
D000841:Anisomycin