Protective effect of a multiantigenic DNA vaccine against Toxoplasma gondii with co-delivery of IL-12 in mice.
In this study, we constructed a multiantigenic DNA vaccine, pSAG1-ROP2-SAG2 and examined its effect with co-delivery of a plasmid encoding IL-12 (pIL-12) as an adjuvant in BALB/c mice against Toxoplasma gondii. After a lethal challenge of T. gondii RH strain, survival of the mice immunized with this pSAG1-ROP2-SAG2 vaccine was significantly prolonged in comparison to the control groups. Furthermore, the protection was significantly augmented by pIL-12 co-delivery. As demonstrated by lymphocyte proliferation assay, cytokine and antibody level determinations, the humoral and Th1-type cellular responses elicited by this multiantigenic DNA vaccine were significantly stronger than those elicited by double-antigenic, or single-antigenic DNA vaccines. Our data suggest that multiantigenic DNA vaccine with pIL-12 co-delivery is a very effective approach in the protection against T. gondii.
Cui
Y-L
YL
Department of Parasitology, School of Medicine, Shandong University, Shandong, China.
He
S-Y
SY
Xue
M-F
MF
Zhang
J
J
Wang
H-X
HX
Yao
Y
Y
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D007136:Immunoglobulins / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology; D018664:Interleukin-12 / Q000494:pharmacology*; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines / Q000276:immunology* / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D019444:Vaccines, DNA / Q000276:immunology* / Q000494:pharmacology
D000953:Antigens, Protozoan; D016207:Cytokines; D007136:Immunoglobulins; D007155:Immunologic Factors; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C090555:ROP 2 protein, Toxoplasma gondii; C047168:SAG1 antigen, Toxoplasma; D019444:Vaccines, DNA; C067547:surface antigen P22, Toxoplasma; D018664:Interleukin-12