Colorectal cancer progression correlates with upregulation of S100A11 expression in tumor tissues.
OBJECTIVE
Early detection and treatment of human colorectal cancers remain a challenge. Identification of new potential markers may help in the diagnosis of colorectal cancer.


METHODS
By comparative two-dimensional gel electrophoresis using extracts from colorectal tumor and adjacent normal tissues, we identified a calcium-binding protein, S100A11, which was highly expressed in colorectal cancer compared with adjacent normal tissues. We expanded our study in 89 clinical colorectal tumor samples to validate this finding and correlates S100A11 expression in human colorectal cancer tissues with various stages of the tumor by Western blotting and immunohistochemical staining.


RESULTS
We identified a calcium-binding protein, S100A11, which was highly expressed in colorectal cancer compared with adjacent normal tissues. S100A protein was expressed predominantly in the cytoplasm of normal tissue; however, it was expressed in both the nuclei and cytoplasm of colorectal cancer. S100A11 level in colorectal cancer tissue was increased following stage progression of the disease.


CONCLUSIONS
These findings suggest S100A11 could be helpful in the pathological study of colorectal cancer, especially for the classification of different stages in colorectal cancer.
Wang
Guiyu
G
Department of Abdominal Surgery, The Affiliated Tumor Hospital, Harbin Medical University, Harbin 150086, China.
Wang
Xishan
X
Wang
Shuhuai
S
Song
Hongtao
H
Sun
Haiming
H
Yuan
Weiguang
W
Cao
Bo
B
Bai
Jing
J
Fu
Songbin
S
D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009367:Neoplasm Staging; D021381:Protein Transport; D009418:S100 Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D015854:Up-Regulation
D009418:S100 Proteins; C072337:S100A11 protein, human