title: Stir-baked Fructus gardeniae (L.) extracts inhibit matrix metalloproteinases and alter cell morphology.
abstract: Matrix metalloproteinases (MMPs) play vital roles in many pathological conditions, including cancer, cardiovascular disease, arthritis and inflammation. Modulating MMP activity may therefore be a useful therapeutic approach in treating these diseases. Qing-Kai-Ling is a popular Chinese anti-inflammatory formulation used to treat symptoms such as rheumatoid arthritis, acute hypertensive cerebral hemorrhage, hepatitis and upper respiratory tract infection. In this paper, we report that one of the components of Qing-Kai-Ling, Fructus gardeniae, strongly inhibits MMP activity. The IC50 values for the primary herbal extract and water extract against MMP-16 were 32 and 27 microg/ml, respectively. In addition, we show that the herbal extracts influence HT1080 human fibrosarcoma cell growth and morphology. These data may provide molecular mechanisms for the therapeutic effects of Qing-Kai-Ling and herbal medicinal Fructus gardeniae.
authors.affiliation: Key laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, Jilin University, Changchun 130023, PR China.
authors.forename: Jin-gang
authors.initials: JG
authors.lastname: Yang
authors.forename: Ye-hua
authors.initials: YH
authors.lastname: Shen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hong
authors.forename: Feng-hai
authors.initials: FH
authors.lastname: Jin
authors.forename: Shu-hua
authors.initials: SH
authors.lastname: Zhao
authors.forename: Ming-cui
authors.initials: MC
authors.lastname: Wang
authors.forename: Xiu-juan
authors.initials: XJ
authors.lastname: Shi
authors.forename: Xue-xun
authors.initials: XX
authors.lastname: Fang
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D032062:Gardenia / Q000737:chemistry*; D006801:Humans; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011480:Protease Inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D004365:Drugs, Chinese Herbal; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; C504928:Qingkailing; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue