The opposing effects of the flavonoids isoquercitrin and sissotrin, isolated from Pterospartum tridentatum, on oral glucose tolerance in rats.
The effect of an aqueous extract of Pterospartum tridentatum on the blood glucose levels of normal Wistar rats was investigated in a situation of oral glucose challenge. The extract at 300 mg/kg showed an antihyperglycaemic effect in the first 30 min after glucose challenge but then the blood glucose levels rose above those of the control group, indicating the presence of compounds with different effects on glucose tolerance. Nine compounds of isoflavone and flavonol skeletons were identified in the extract by HPLC-ESI-MS(n), four of them being identified for the first time in this species. The isoflavone sissotrin and the flavonol derivative, isoquercitrin, were selected for the oral glucose tolerance test. Isoquercitrin (100 mg/kg) showed time-dependent antihyperglycaemic activity by delaying the post-oral glucose load glycaemic peak at 30 min, as did the sodium-dependent glucose transporter inhibitor phloridzin (100 mg/kg). In contrast, sissotrin (100 mg/kg) showed an opposite effect, impairing glucose tolerance. In conclusion, these preliminary results indicate that the effect of the extract on blood glucose may be either antihyperglycaemic or hyperglycaemic. Additionally, as far as is known, these are the first in vivo results on the acute antihyperglycaemic potential of isoquercitrin.
Paulo
Alexandra
A
iMed/CECF, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal. mapaulo@ff.ul.pt
Martins
Sofia
S
Branco
Pedro
P
Dias
Teresa
T
Borges
Carlos
C
Rodrigues
Ana Isabel
AI
Costa
Maria do Céu
Mdo C
Teixeira
Adriano
A
Mota-Filipe
Hélder
H
D000818:Animals; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005951:Glucose Tolerance Test; D015394:Molecular Structure; D010695:Phlorhizin / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization
D001786:Blood Glucose; D005419:Flavonoids; D010936:Plant Extracts; C016527:isoquercitrin; D011794:Quercetin; D010695:Phlorhizin