Protective effects of Terminalia arjuna against Doxorubicin-induced cardiotoxicity.
Terminalia arjuna has been marked as a potential cardioprotective agent since vedic period. The present study was aimed to investigate the effects of butanolic fraction of Terminalia arjuna bark (TA-05) on Doxorubicin (Dox)-induced cardiotoxicity. Male wistar rats were used as in vivo model for the study. TA-05 was administered orally to Wistar rats at different doses (0.42 mg/kg, 0.85 mg/kg, 1.7 mg/kg, 3.4 mg/kg and 6.8 mg/kg) for 6 days/week for 4 weeks. Thereafter, all the animals except saline and TA-05-treated controls were administered 20 mg/kg Dox intraperitonially. There was a significant decrease in myocardial superoxide dismutase (38.94%) and reduced glutathione (23.84%) in animals treated with Dox. Concurrently marked increase in serum creatine kinase-MB (CKMB) activity (48.11%) as well as increase in extent of lipid peroxidation (2.55-fold) was reported. Co-treatment of TA-05 and Dox resulted in an increase in the cardiac antioxidant enzymes, decrease in serum CKMB levels and reduction in lipid peroxidation as compared to Dox-treated animals. Electron microscopic studies in Dox-treated animals revealed mitochondrial swelling, Z-band disarray, focal dilatation of smooth endoplasmic reticulum (SER) and lipid inclusions, whereas the concurrent administration of TA-05 led to a lesser degree of Dox-induced histological alterations. These findings suggest that butanolic fraction of Terminalia arjuna bark has protective effects against Dox-induced cardiotoxicity and may have potential as a cardioprotective agent.
Singh
Gurvinder
G
Dabur Research Foundation, 22 Site IV, Sahibabad, Ghaziabad 201010, U.P., India.
Singh
Anu T
AT
Abraham
Aji
A
Bhat
Beena
B
Mukherjee
Ashok
A
Verma
Ritu
R
Agarwal
Shiv K
SK
Jha
Shivesh
S
Mukherjee
Rama
R
Burman
Anand C
AC
D000818:Animals; D002374:Catalase / Q000378:metabolism; D052279:Creatine Kinase, MB Form / Q000097:blood; D004317:Doxorubicin / Q000633:toxicity*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D029766:Terminalia / Q000737:chemistry
D016166:Free Radical Scavengers; D010936:Plant Extracts; D004317:Doxorubicin; D002374:Catalase; D013482:Superoxide Dismutase; D052279:Creatine Kinase, MB Form; D005978:Glutathione