title: Mutations in the beta1 adrenergic receptor gene and massive obesity in Japanese.
abstract: Catecholamines strongly promote lipolysis and thermogenesis, and play a central role in the regulation of body fat content. The beta1 adrenergic receptor (BAR-1) is a major mediator of catecholamine-induced lipolysis and thermogenesis. To explore whether mutations in the BAR-1 gene contribute to morbid obesity in Japanese, we scanned for mutations in the coding sequence of the gene in 50 morbid obese [body mass index (BMI)>==35.0kg/m(2); 99.7th percentile] Japanese subjects. Direct DNA sequencing was performed following polymerase chain reaction (PCR) amplification. Two common polymorphisms, Gly49Arg and Arg389Ser, were detected in these subjects. The frequencies of these polymorphisms, as determined by PCR-restriction fragment length polymorphism (RFLP) analysis, showed no significant difference between 180 severely obese subjects (BMI>==30.0kg/m(2); 97th percentile) and 132 control (BMI<25.0kg/m(2)) subjects. This study represents the first investigations of genetic variations of BAR-1 in relationship to morbid obesity and suggests mutations in the BAR-1 coding sequence is not likely a major cause of morbid obesity at least in Japanese.
authors.affiliation: The First Department of Medicine, Wakayama Medical University, 811-1 Kimi-idera, Wakayama 641-8509, Japan.
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Ohshiro
authors.forename: Mina
authors.initials: M
authors.lastname: Hayashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Yabiku
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ueda
authors.forename: Hisao
authors.initials: H
authors.lastname: Wakasaki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishigame
authors.forename: Hiroto
authors.initials: H
authors.lastname: Furuta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Sasaki
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takasu
authors.forename: Kishio
authors.initials: K
authors.lastname: Nanjo
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D044466:Asian People / Q000235:genetics; D017931:DNA Primers; D014644:Genetic Variation; D006720:Homozygote; D006801:Humans; D007564:Japan; D023421:Models, Animal; D009154:Mutation; D009765:Obesity / Q000235:genetics*; D009767:Obesity, Morbid / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D051381:Rats; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018342:Receptors, Adrenergic, beta-1