title: Assessment of efflux pump gene expression in a clinical isolate Mycobacterium tuberculosis by real-time reverse transcription PCR.
abstract: The importance of some putative efflux pumps in conferring drug resistance on certain strain of Mycobacterium tuberculosis was determined by using multidrug-resistant (MDR) strain 1499 bearing defined mutations in rpoB and katG. The clinical isolate 1499 was tested for drug sensitivity and expression level of five putative efflux pump genes by real-time RT-PCR. In the presence of rifampicin or isoniazid, this isolate showed increased transcription of putative efflux pump genes Rv1258c and Rv1410c. Rv1819c was overexpressed only upon isoniazid exposure (p<0.05). Overexpression of some efflux genes is associated with multidrug resistance in certain clinical isolate of M. tuberculosis.
authors.affiliation: Shanghai University of T.C.M., Shanghai, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Wenhong
authors.initials: W
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Chanyi
authors.initials: C
authors.lastname: Lu
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zhang
authors.forename: Honghai
authors.initials: H
authors.lastname: Wang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis / Q000235:genetics; D000995:Antitubercular Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D015373:Bacterial Typing Techniques; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology
chemical_list: D018528:ATP-Binding Cassette Transporters; D000995:Antitubercular Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C425143:P55 protein, Mycobacterium; C519050:Rv1258c protein, Mycobacterium tuberculosis; D019883:Alkyl and Aryl Transferases; C499606:Rv2361c protein, Mycobacterium tuberculosis; D007538:Isoniazid; D012293:Rifampin