Caveolin-1 sensitizes vascular smooth muscle cells to mildly oxidized LDL-induced apoptosis.
Oxidized low-density lipoprotein (oxLDL)-induced apoptosis of vascular cells may participate to plaque instability and rupture. Caveolin-1 has emerged as an important regulator of several signal transduction pathways and processes that play a role in atherosclerosis. In this study we examined the potential role of caveolin-1 in the regulation of oxLDL-induced Ca(2+) signaling and apoptosis in vascular smooth muscle cells (VSMC). Cells expressing caveolin-1 were more susceptible to oxLDL-induced apoptosis, and this was correlated with enhanced Ca(2+) entry and pro-apoptotic events. Moreover, caveolin-1 silencing by small interfering RNA decreased the level of apoptotic cells after oxLDL treatment. These findings provide new insights about the potential role of caveolin-1 in the regulation of oxLDL-induced apoptosis in vascular cells and its contribution to the instability of the plaque.
Ingueneau
Cécile
C
INSERM U-858/I2MR, Department of Vascular Biology, IFR-31, CHU Rangueil, 1 Avenue Jean Poulhes, BP 84225, 31432 Toulouse Cedex 4, France.
Huynh-Do
Uyen
U
Thiers
Jean-Claude
JC
Nègre-Salvayre
Anne
A
Salvayre
Robert
R
Vindis
Cécile
C
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D051242:Caveolin 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000633:toxicity*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D034741:RNA, Small Interfering / Q000494:pharmacology; D011817:Rabbits
D051242:Caveolin 1; D008077:Lipoproteins, LDL; D034741:RNA, Small Interfering; C099252:oxidized low density lipoprotein; D002118:Calcium