title: Magnetic separation: a highly effective method for synchronization of cultured erythrocytic Plasmodium falciparum.
abstract: The magnetic method has been previously utilized to concentrate Plasmodium-infected erythrocytes without any significant influence on the viability of the parasite. We attempted, in this study, to concentrate and synchronize cultivated P. falciparum via the magnetic method. The results of this study showed that the magnetic method effectively synchronized and concentrated P. falciparum with finer demarcation capacity in the erythrocytic asexual cycle of the parasite than currently available synchronization methods. Concentration and synchronization by the magnetic method proved most effective when schizonts were dominant. Therefore, it proved necessary to enhance the synchronization efficiency of the magnetic method by first applying the method currently in use, which renders schizonts dominant. Our study also showed that the intrinsic life cycle of erythrocytic P. falciparum was slightly longer than 48 h observed in natural infection cases, and that the length of the intrinsic life cycles between various P. falciparum strains differed slightly.
authors.affiliation: Department of Microbiology, Gachon Medical School, 1198, Kuwol-1-dong, Namdong-gu, Incheon 405-760, Republic of Korea. ansy0207@hanmail.net
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Ahn
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Shin
authors.forename: Young-A
authors.initials: YA
authors.lastname: Kim
authors.forename: Ji-Ae
authors.initials: JA
authors.lastname: Yoo
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kwak
authors.forename: Yun-Jae
authors.initials: YJ
authors.lastname: Jung
authors.forename: Gyo
authors.initials: G
authors.lastname: Jun
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Ryu
authors.forename: Joon-Sup
authors.initials: JS
authors.lastname: Yeom
authors.forename: Jeong-Yeal
authors.initials: JY
authors.lastname: Ahn
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Park
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000469:parasitology*; D008280:Magnetics; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000302:isolation & purification*; D052939:Schizonts / Q000254:growth & development