Regulation of MUC16 by inflammatory mediators in ocular surface epithelial cell lines.
The aim of the present study was to evaluate the regulation of membrane-anchored mucin MUC16 by proinflammatory cytokines and bacterial components at the ocular surface. Expression and distribution of MUC16 in conjunctival (HCjE) and corneal (HCE) epithelial cell lines was monitored by RT-PCR and immunohistochemistry. To determine the regulation of MUC16, cultured HCjEs and HCEs were stimulated with different cytokines, bacterial components and bacterial supernatants, and analyzed by real-time PCR, immunodot blot and immunohistochemistry. The results indicate that MUC16 is differentially regulated between HCjEs and HCEs after challenge with inflammatory mediators and suggest shedding of MUC16 from the ocular surface epithelia into the tear film. This seems to be precisely regulated. MUC16 shedding can be differentially increased and decreased, suggesting a protective function of membrane-anchored MUC16 and supporting the hypothesis that dysregulation of membrane-anchored MUC16 at the ocular surface may be involved in dry eye pathology.
Paulsen
Friedrich
F
Department of Anatomy and Cell Biology, Martin-Luther-University Halle-Wittenberg, Grosse Steinstr. 52, D-06097 Halle/Saale, Germany. friedrich.paulsen@medizin.uni-halle.de
Jäger
Kristin
K
Worlitzsch
Dieter
D
Bräuer
Lars
L
Schulze
Ute
U
Schäfer
Gesa
G
Sel
Saadettin
S
D018394:CA-125 Antigen / Q000235:genetics*; D002460:Cell Line; D003228:Conjunctiva / Q000166:cytology* / Q000502:physiology; D003315:Cornea / Q000166:cytology* / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000503:physiopathology; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics*; D010457:Peptidoglycan / Q000494:pharmacology; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D018394:CA-125 Antigen; D018076:DNA, Complementary; D007375:Interleukin-1; D008070:Lipopolysaccharides; C451759:MUC16 protein, human; D008565:Membrane Proteins; D010457:Peptidoglycan; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D007371:Interferon-gamma