title: Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules.
abstract: A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.
authors.affiliation: Department of Chemistry and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA.
authors.forename: Tudor P
authors.initials: TP
authors.lastname: Constantin
authors.forename: Gloria L
authors.initials: GL
authors.lastname: Silva
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Robertson
authors.forename: Tamara P
authors.initials: TP
authors.lastname: Hamilton
authors.forename: Kaitlin
authors.initials: K
authors.lastname: Fague
authors.forename: Alan S
authors.initials: AS
authors.lastname: Waggoner
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Armitage
mesh_terms: D002232:Carbocyanines / Q000138:chemical synthesis* / Q000737:chemistry; D005461:Fluorine / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D002232:Carbocyanines; D005461:Fluorine; D012313:RNA