Developmental expression of glycine receptor subunits in rat cerebellum.
The distribution of the glycine receptor subunits alpha1-3 and beta in the developing rat cerebellum was studied from postnatal day 1 to adulthood by means of quantitative RT-PCR and immunohistochemistry. qRT-PCR of postnatal cerebella indicated the presence of mRNA for each subunit, with a relative expression of alpha2>alpha3>alpha1>beta. The immunohistochemistry indicated a strong alpha2 signal in the Purkinje cells, internal and external granular layers. The alpha1-3 subunits had weak signals in the Purkinje cells and molecular layer. The alpha1 subunit was expressed at a low level and was also found in the white matter. The function of these receptors in neuronal and glial plasma membranes in early postnatal development remains to be determined.
García-Alcocer
Guadalupe
G
Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Querétaro, Mexico. leguga@email.com
Mejía
Carmen
C
Berumen
Laura C
LC
Miledi
Ricardo
R
Martínez-Torres
Ataúlfo
A
D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005998:Glycine / Q000378:metabolism*; D007150:Immunohistochemistry; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018009:Receptors, Glycine / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions
C501174:Glra2 protein, rat; C491948:Glrb protein, rat; D021122:Protein Subunits; D012333:RNA, Messenger; D018009:Receptors, Glycine; D005998:Glycine