title: The Saccharomyces cerevisiae Wss1 protein is only present in mother cells.
abstract: The Saccharomyces cerevisiae WSS1 (Weak Suppressor of Smt3) gene has initially been identified as a multicopy suppressor of a mutation in SMT3 encoding the small ubiquitin-like modifier. Later, multiple functions related to DNA replication and repair have been found for WSS1. Here, we report the subcellular location of the Wss1 protein. Fluorescence microscopy of strains expressing a Wss1p-green fluorescent protein (GFP) fusion shows that the protein is present in a single sharp spot near the nuclear membrane, distinct from the spindle pole bodies and nucleolus. In dividing cells, the spot is exclusively present in the mother cell, suggesting a mother cell-specific function of WSS1.
authors.affiliation: Section Molecular and Developmental Genetics, Institute of Biology, Leiden University, AL Leiden, The Netherlands. g.p.h.van.heusden@biology.leidenuniv.nl
authors.forename: G Paul H
authors.initials: GP
authors.lastname: van Heusden
authors.forename: H Yde
authors.initials: HY
authors.lastname: Steensma
mesh_terms: D002455:Cell Division; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D053484:RecQ Helicases / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C494617:WSS1 protein, S cerevisiae; D049452:Green Fluorescent Proteins; C090472:SGS1 protein, S cerevisiae; D053484:RecQ Helicases