[Replication of duck plague virus in artificially infected ducks detected by in situ hybridization].
Replication of duck plague virus(DPV) in artificially infected ducks were detected by in situ hybridization (ISH) which employed a 37bp oligonucleotide as probe designed according to DPV DNA sequence in GenBank. The results indicated that DPV DNA was detected in liver, intestine and bursa Fabricius at 4 h, in spleen and esophagus at 6h, in thymus at 12h post infection; DPV DNA in lung and kidney was detected only in dead ducks and no positive signal was detected in muscle, heart, cerebrum and pancreas. DPV DNA was distributed in cell nucleus and cytoplasm. Hepatocytes, sinus endodermal cells and Kuffer's cells were the mainly infected cell types in liver. DPV DNA was mainly detected in epithelium of villi, in lamina propria of intestinal villi of duodenum, in stratum spinosum of esophagus, and in epithelium, cortex, medulla of bursa Fabricius. The positive signals were mainly detected in medulla of thymus, lymphocytes and macrophages of spleen. The research suggests that ISH is a direct and specific method in detecting DPV DNA in paraffin sections and it's also a good method for virus diagnosis and DNA location of DPV.
Cheng
An-Chun
AC
Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Yaan 625014, China. chenganchun@vip.163.om
Liao
Yong-Hong
YH
Zhu
De-Kang
DK
Wang
Ming-Shu
MS
Yuan
Gui-Ping
GP
Xu
Chao
C
Han
Xiao-Ying
XY
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004372:Ducks / Q000821:virology*; D017403:In Situ Hybridization; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014779:Virus Replication
D004279:DNA, Viral