A semi-defined medium without serum for small ruminant mycoplasmas.
The composition of the medium used to cultivate Mycoplasma species is very important. Serum is one of the most important additives as it contains lipids (cholesterol) and serum proteins, which are essential for the growth of the organisms. This work reports the development of a semi-defined medium, called MWS (Medium Without Serum) produced without animal serum and bovine serum albumin. MWS seems to be suitable for cultivating several species of caprine mycoplasma, especially M. mycoides subsp. mycoides (LC) and M. mycoides subsp. capri.
Ramírez
Ana S
AS
Unidad de Epidemiología y Medicina Preventiva, Spain. aramirez@dpat.ulpgc.es
Fleitas
Juan Luis
JL
Rosales
Rubén S
RS
Poveda
Carlos
C
de la Fe
Christian
C
Andrada
Marisa
M
Castro
Ayoze
A
Poveda
José B
JB
D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D016895:Culture Media, Serum-Free / Q000737:chemistry*; D009174:Mycoplasma / Q000302:isolation & purification*; D012418:Ruminants / Q000382:microbiology*
D016895:Culture Media, Serum-Free