Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE).
One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.
Deutsch
Eric W
EW
Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103, USA.
Ball
Catherine A
CA
Berman
Jules J
JJ
Bova
G Steven
GS
Brazma
Alvis
A
Bumgarner
Roger E
RE
Campbell
David
D
Causton
Helen C
HC
Christiansen
Jeffrey H
JH
Daian
Fabrice
F
Dauga
Delphine
D
Davidson
Duncan R
DR
Gimenez
Gregory
G
Goo
Young Ah
YA
Grimmond
Sean
S
Henrich
Thorsten
T
Herrmann
Bernhard G
BG
Johnson
Michael H
MH
Korb
Martin
M
Mills
Jason C
JC
Oudes
Asa J
AJ
Parkinson
Helen E
HE
Pascal
Laura E
LE
Pollet
Nicolas
N
Quackenbush
John
J
Ramialison
Mirana
M
Ringwald
Martin
M
Salgado
David
D
Sansone
Susanna-Assunta
SA
Sherlock
Gavin
G
Stoeckert
Christian J
CJ
Swedlow
Jason
J
Taylor
Ronald C
RC
Walashek
Laura
L
Warford
Anthony
A
Wilkinson
David G
DG
Zhou
Yi
Y
Zon
Leonard I
LI
Liu
Alvin Y
AY
True
Lawrence D
LD
D019295:Computational Biology / Q000379:methods / Q000592:standards; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards; D007150:Immunohistochemistry / Q000379:methods / Q000592:standards*; D017403:In Situ Hybridization / Q000379:methods / Q000592:standards*