title: Characterization of tomato zonate spot virus, a new tospovirus in China.
abstract: An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. The complete nucleotide sequences of large (L), medium (M), and small (S) RNAs of this virus were found to contain 8919, 4945, and 3279 nts respectively. The L RNA encoded the RNA-dependent RNA polymerase (RdRp) (2885 aa, 332.7 kDa). The M RNA encoded a non-structural (NSm) protein (309 aa, 34.4 kDa) and a viral glycoprotein precursor (Gn/Gc) (1122 aa, 127.4 kDa). The S RNA encoded a non-structural protein (NSs) (459 aa, 51.9 kDa) and the nucleocapsid (N) protein (278 aa, 30.6 kDa). This N protein shared amino acid identities of 80.9% with those of calla lily chlorotic spot virus. Our results suggest that the virus studied here belongs to a new tospovirus species, for which the name tomato zonate spot virus is proposed.
authors.affiliation: Yunnan Provincial Key Laboratory of Agri-Biotechnology, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650223, People's Republic of China.
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Dong
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Cheng
authors.forename: Yue-Yan
authors.initials: YY
authors.lastname: Yin
authors.forename: Qi
authors.initials: Q
authors.lastname: Fang
authors.forename: Ming
authors.initials: M
authors.lastname: Ding
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Li
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Zhang
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Su
authors.forename: Jenifer Huang
authors.initials: JH
authors.lastname: McBeath
authors.forename: Zhong-Kai
authors.initials: ZK
authors.lastname: Zhang
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D002212:Capsicum / Q000821:virology; D002681:China; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D018551:Solanum lycopersicum / Q000821:virology*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D009626:Terminology as Topic; D017865:Tospovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D014764:Viral Proteins