Visualization of growth signal transduction cascades in living cells with genetically encoded probes based on Förster resonance energy transfer.
Fluorescence probes based on the principle of Förster resonance energy transfer (FRET) have shed new light on our understanding of signal transduction cascades. Among them, unimolecular FRET probes containing fluorescence proteins are rapidly increasing in number because these genetically encoded probes can be easily loaded into living cells and allow simple acquisition of FRET images. We have developed probes for small GTPases, tyrosine kinases, serine-threonine kinases and phosphoinositides. Images obtained with these probes have revealed that membrane protrusions such as nascent lamellipodia or neurites provide an active signalling platform in the growth factor-stimulated cells.
Aoki
Kazuhiro
K
Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Kiyokawa
Etsuko
E
Nakamura
Takeshi
T
Matsuda
Michiyuki
M
D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D020558:GTP Phosphohydrolases / Q000378:metabolism; D008958:Models, Molecular; D015336:Molecular Probe Techniques; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
D005456:Fluorescent Dyes; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D020558:GTP Phosphohydrolases