title: Improved performance of enzyme-linked immunosorbent assays and the effect of human immunodeficiency virus coinfection on the serologic detection of herpes simplex virus type 2 in Rakai, Uganda.
abstract: Ugandan subjects (820) were tested by Focus HerpeSelect enzyme-linked immunosorbent assay (ELISA), Kalon herpes simplex virus type 2 ELISA, and BioKit rapid test, and the results were compared to those of Western blotting. Higher-than-standard-index cutoff values gave optimal sensitivity and specificity. Kalon ELISA was the optimal assay when an index value of 1.5 was used (sensitivity, 91.7%; specificity, 92.4%).
authors.affiliation: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Jordyn L
authors.initials: JL
authors.lastname: Gamiel
authors.forename: Aaron A R
authors.initials: AA
authors.lastname: Tobian
authors.forename: Oliver B
authors.initials: OB
authors.lastname: Laeyendecker
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Reynolds
authors.forename: Rhoda Ashley
authors.initials: RA
authors.lastname: Morrow
authors.forename: David
authors.initials: D
authors.lastname: Serwadda
authors.forename: Ronald H
authors.initials: RH
authors.lastname: Gray
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Quinn
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000150:complications; D006558:Herpes Genitalis / Q000175:diagnosis* / Q000453:epidemiology; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014454:Uganda / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic