[Percutaneous lumbar interbody fusion using autogenous osteoblasts in vivo].
OBJECTIVE
In the present study we have investigated the potential of autogenous osteoblasts for osteoinduction in a spinal arthrodesis model.


METHODS
After posterolateral instrumentation of 3 segments of a sheep spine the intervertebral space was filled with cancellous bone, osteoblasts or left empty after nucleotomy and elimination of cartilage.


RESULTS
Radiological and histological analyses proved a significant osseous reaction in segments treated with cancellous bone or osteoblasts in contrast to the control segment. This outcome provides evidence of spondylodeses by spinal instrumentation in combination with osteoblasts in a sheep model.


CONCLUSIONS
The results suggest a possible application of autologous osteoblasts as an osteoinductor after percutaneous nucleotomy in spinal fusion. The possible application in a human model should be examined.
Berghof
L
L
Orthopädische Klinik und Poliklinik der Universität Duisburg-Essen, Essen. siahkamary@web.de
Bolle
I
I
Schulz
A
A
Grifka
J
J
Quint
U
U
D000818:Animals; D016723:Bone Remodeling / Q000502:physiology; D001863:Bone Screws; D016025:Bone Transplantation; D005260:Female; D007403:Intervertebral Disc / Q000473:pathology / Q000601:surgery; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000473:pathology / Q000601:surgery*; D019060:Minimally Invasive Surgical Procedures / Q000295:instrumentation / Q000379:methods*; D010006:Osteoblasts / Q000473:pathology / Q000637:transplantation*; D011859:Radiography; D012756:Sheep; D013123:Spinal Fusion / Q000295:instrumentation / Q000379:methods*