[Determination of iridoids and triterpenes in herb of Swertia pseudochinesis by RP-HPLC].
OBJECTIVE
To establish a RP-HPLC method for the determination of swertiamarin, sweroside, gentiopicrin and oleanolic acid in different parts of Swertia pseudochinesis.


METHODS
A Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm) column was used with acetonitrile-water (10:90) and methnol-water(86:14) at detection wavelengths of 238 nm, 246 nm, 274 nm and 207 nm for swertiamarin, sweroside, gentiopicrin and oleanolic acid respectively. The flow rate was 1.0 mL x min(-1) and the column temperature was 25 degrees C.


RESULTS
All of the compounds were based--isolated. The linear ranges of swertiamarin, sweroside, gentiopicrin and oleanolic acid were 0.068 9-0.344 4(r = 0.999 2) , 0.001 1-0.014 0 (r2 = 0. 999 8), 0.001 1-0.013 4 (r2 = 0.999 9) and 0.001 1-0.008 8 mg x mL(-1) (r2 = 0. 999 6), respectively.


CONCLUSIONS
The method is simple and accurate, which can be used for quality control of S. pseudochinesis.
Huang
Wen-Zhe
WZ
State Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100075, China.
Xu
Chun-Hui
CH
Zhou
Da-Cheng
DC
Tong
Li-Hua
LH
Ye
Zu-Guang
ZG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D035264:Flowers / Q000737:chemistry; D005960:Glucosides / Q000032:analysis; D057888:Iridoid Glucosides; D039823:Iridoids / Q000032:analysis*; D009828:Oleanolic Acid / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011753:Pyrones / Q000032:analysis; D015203:Reproducibility of Results; D029967:Swertia / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*
D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011753:Pyrones; D014315:Triterpenes; C013270:swertiamarin; D009828:Oleanolic Acid; C049412:sweroside