Oxygen decline in biotesting of environmental samples--is there a need for consideration in the acute zebrafish embryo assay?
Environmental samples such as groundwater, sediment pore water, native or freeze dried sediments may be difficult to analyze for toxic effects with organismic aquatic bioassays. These samples might evoke low oxygen concentration or oxygen depletion during the test. The toxicity assessment could thus be confounded by low oxygen concentrations. The acute zebrafish embryo assay was used to analyze the influence of oxygen deficit on the embryonic development in the first 48 h post fertilization. Embryos were exposed to varying oxygen concentrations ranging from <30 to >80% oxygen saturation of water. A clear concentration dependent retardation of fish embryo development was observed. Because of a retarded development toxic thresholds of environmental samples which might include substances slowing down the development will be altered. For the purpose of identification of critical contaminants in complex environmental samples, it is proposed to actively aerate environmental samples which are likely to be oxygen depleted during the duration of the zebrafish embryo bioassay.
Küster
Eberhard
E
UFZ, Helmholtz-Centre for Environmental Research, Department Bioanalytical Ecotoxicology, Permoserstr. 15, 04318 Leipzig, Germany. eberhard.kuester@ufz.de
Altenburger
Rolf
R
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D005306:Fertilization / Q000502:physiology; D005618:Fresh Water / Q000737:chemistry; D010100:Oxygen / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D010101:Oxygen Consumption / Q000502:physiology; D018570:Risk Assessment; D013997:Time Factors; D023382:Toxicity Tests, Acute / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism
D014874:Water Pollutants, Chemical; D010100:Oxygen