Bletilla striata polysaccharide stimulates inducible nitric oxide synthase and proinflammatory cytokine expression in macrophages.
Bletilla striata, a traditional Chinese medicine, has been used for the treatment of alimentary canal mucosal damage, ulcers, bleeding, bruises and burns. B. striata polysaccharide (BSP) isolated from B. striata was found to enhance vascular endothelial cell (EC) proliferation and vascular endothelial growth factor (VEGF) expression. However, the wound healing mechanism of BSP is not well understood. In this study, the results show that treatment with BSP induces coordinate changes in inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1beta) mRNA levels and enhances the expression of these cytokines, but has no effect on interferon gamma (IFN-gamma) level. In this study, we partially elucidate the wound healing mechanism of BSP.
Diao
Huajia
H
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, People's Republic of China.
Li
Xin
X
Chen
Jiangning
J
Luo
Yi
Y
Chen
Xi
X
Dong
Lei
L
Wang
Chunming
C
Zhang
Chenyu
C
Zhang
Junfeng
J
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D007155:Immunologic Factors / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000276:immunology*; D029595:Orchidaceae / Q000378:metabolism*; D011134:Polysaccharides / Q000008:administration & dosage*; D014945:Wound Healing / Q000276:immunology* / Q000528:radiation effects
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D011134:Polysaccharides; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse