title: Anatomical pitfalls during encircling cryoablation of the left atrium for atrial fibrillation therapy in the pig.
abstract: We previously published encircling endocardial cryo-isolation of the pulmonary vein (PV) region. This study documented mechanisms of isolation failure using CARTO mapping. Cryo-isolation used a modified Surgifrost introduced via a Universal Cardiac Introducer on the left atrial appendage. Of five pigs, two had incomplete isolation and repeat mapping: Activation was over Bachmann's bundle (BB) in one and the coronary sinus (CS) in the other. Repeat cryoablation failed to eliminate gaps. Histologically, the BB gap had nonlesioned sub-epicardial fibres and thick fat covering the cryolesioned BB: fat protecting the epicardium from cryoablation. The inferior gap had a large CS, and a thick myocardium bridging the isthmus: myocardial thickness and CS thermal sink preventing transmural cryolesions. CARTO mapping localized gaps. Although the CS is known to cause failure, its protective mechanism is not well documented. The BB gap is novel. These findings have important clinical implications for isolation of the PV region.
authors.affiliation: Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario, Canada, N6A 5C1. Doug.Jones@schulich.uwo.ca
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Jones
authors.forename: Gerard M
authors.initials: GM
authors.lastname: Guiraudon
authors.forename: Allan C
authors.initials: AC
authors.lastname: Skanes
authors.forename: Colette M
authors.initials: CM
authors.lastname: Guiraudon
mesh_terms: D000818:Animals; D001281:Atrial Fibrillation / Q000601:surgery*; D017115:Catheter Ablation; D047549:Coronary Artery Bypass, Off-Pump / Q000379:methods; D003452:Cryosurgery / Q000379:methods; D004195:Disease Models, Animal; D022062:Electrophysiologic Techniques, Cardiac / Q000379:methods; D006325:Heart Atria / Q000033:anatomy & histology* / Q000601:surgery; D011667:Pulmonary Veins / Q000601:surgery*; D013552:Swine