Cellular uptake of fluorescent labelled biotin-streptavidin microspheres.
Amino functionalized, cross-linked, polystyrene microspheres were covalently loaded with streptavidin to which was coupled fluorescently labeled biotin and biotinylated-tagged DNA. These biotin-streptavidin microsphere conjugates were then successfully delivered into cells. The application of the streptavidin-biotin technology to these microspheres allows the effective delivery of any biotinylated material into intact mammalian cells, without the need for delicate procedures such as micro-injection.
Bradley
M
M
Chemical Biology Section, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, UK.
Alexander
L
L
Sanchez-Martin
R M
RM
D001692:Biological Transport; D001710:Biotin / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D004705:Endocytosis; D005434:Flow Cytometry; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry*
D002232:Carbocyanines; D004337:Drug Carriers; D005456:Fluorescent Dyes; D011137:Polystyrenes; C085321:cyanine dye 5; D001710:Biotin; D004247:DNA; D019809:Streptavidin; D019793:Fluorescein