Induction of apoptosis by recombinant soluble human TRAIL in Jurkat cells.
OBJECTIVE
To investigate the therapeutic potential of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, and to analyze TRAIL-induced apoptosis in Jurkat cells.


METHODS
Expression of TRAIL receptors (DR4 and DR5) was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Cytotoxic effects were determined by colony formation assay and a cell counting kit. The effects of recombinant TRAIL on apoptosis of Jurkat cells were determined by DNA fragmentation (DNA ladder) and PI staining. Changes in mitochondrial membrane potential were detected with JC-1 fluorescence.


RESULTS
TRAIL inhibited the proliferation and induced internucleosomal DNA fragmentation (characteristic of apoptosis) and loss of mitochondrial membrane potential.


CONCLUSIONS
Recombinant soluble TRAIL can be used as a therapy for cancer.
Yao
Gen-Hong
GH
Department of Transfusion, Jinling Hospital, Medical School, Nanjing University, Nanjing 210002, Jiangsu, China. ygh71@tom.com
Ling
Li-Jun
LJ
Luan
Jian-Feng
JF
Ye
Dong
D
Zhu
Pei-Yuan
PY
Lei
Qian-Hong
QH
D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D005453:Fluorescence; D006801:Humans; D019169:Jurkat Cells; D008564:Membrane Potentials; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012995:Solubility; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology*
D017931:DNA Primers; D011994:Recombinant Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human