title: [Rapid determination of residues of 4 tetracyclines in meat by a microbiological screening, HPLC and LC/MS/MS].
abstract: A rapid and precise determination residues of 4 tetracyclines (TCs) (oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC) and doxycycline (DOXY)) in meat was developed by employing three analyses; a microbiological screening, HPLC and LC/MS/MS. TCs were extracted with pH 4.0 McIlvaine buffer containing 0.01 mol/L EDTA from a meat sample, and then purified using a mixed mode, reversed-phase and cation-exchange cartridge. The mean recoveries (n=5) of 0.2 microg/g OTC, TC and CTC, 0.05 microg/g DOXY spiked in meat samples were 76.6-99.0% (C.V. 1.6-5.4%). In 13 meat samples in which the microbiological screening indicated the presence of TCs, CTC (9 samples) and DOXY (4 samples) were identified with HPLC and LC/MS/MS.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kanda
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kusano
authors.forename: Taka
authors.initials: T
authors.lastname: Osanai
authors.forename: Keiko
authors.initials: K
authors.lastname: Ushiyama
authors.forename: Kazue
authors.initials: K
authors.lastname: Takeba
authors.forename: Miho
authors.initials: M
authors.lastname: Sakamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hayashi
authors.forename: Kyoko
authors.initials: K
authors.lastname: Igusa
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ibe
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nagayama
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D002645:Chickens; D002751:Chlortetracycline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004318:Doxycycline / Q000032:analysis; D008460:Meat / Q000032:analysis*; D010118:Oxytetracycline / Q000032:analysis; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis*
chemical_list: D013754:Tetracyclines; D004318:Doxycycline; D002751:Chlortetracycline; D010118:Oxytetracycline