Efficient stem cell labeling for MRI studies.
Iron oxide particles are especially suited for cell tracking experiments due to their extraordinarily molar relaxivity as compared with other paramagnetic nuclei. We have compared different iron oxide particles (Sinerem, Endorem and magnetic microspheres) for their suitability to label embryonic stem cells (D3 cell line). In addition to detectability thresholds, particular attention has been paid to the evaluation of long-term stability of the labelling procedure (up to 4 weeks) as well as to toxic and other adverse effects on cell viability. Comparative studies were performed using neural progenitor cells (C17.2) and dendritic cells. The present study indicates strong dependence of the label efficiency and stability on the iron oxide particles and cell lines in use.
Küstermann
E
E
Max-Planck-Insitute for Neurological Research with Klaus-Joachim-Zülch-Laboratories of the Max-Planck-Society and the Faculty of Medicine of the University of Cologne, Germany.
Himmelreich
U
U
Kandal
K
K
Geelen
T
T
Ketkar
A
A
Wiedermann
D
D
Strecker
C
C
Esser
J
J
Arnhold
S
S
Hoehn
M
M
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000009:adverse effects / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology*; D004355:Drug Stability; D053595:Embryonic Stem Cells / Q000166:cytology*; D005290:Ferric Compounds / Q000009:adverse effects / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging; D051379:Mice; D013997:Time Factors
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide