title: Autoregulation of the Escherichia coli melR promoter: repression involves four molecules of MelR.
abstract: The Escherichia coli MelR protein is a transcription activator that autoregulates its own promoter by repressing transcription initiation. Optimal repression requires MelR binding to a site that overlaps the melR transcription start point and to upstream sites. In this work, we have investigated the different determinants needed for optimal repression and their spatial requirements. We show that repression requires a complex involving four DNA-bound MelR molecules, and that the global CRP regulator plays little or no role.
authors.affiliation: School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Shivanthi
authors.initials: S
authors.lastname: Samarasinghe
authors.forename: Mohamed Samir
authors.initials: MS
authors.lastname: El-Robh
authors.forename: David C
authors.initials: DC
authors.lastname: Grainger
authors.forename: Wenke
authors.initials: W
authors.lastname: Zhang
authors.forename: Panos
authors.initials: P
authors.lastname: Soultanas
authors.forename: Stephen J W
authors.initials: SJ
authors.lastname: Busby
mesh_terms: D001665:Binding Sites; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D015964:Gene Expression Regulation, Bacterial; D006706:Homeostasis; D018625:Microscopy, Atomic Force; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002373:Cyclic AMP Receptor Protein; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C077649:MelR protein, E coli; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C481662:crp protein, E coli