Fatty acids influence binding of cobalt to serum albumin in patients with fatty liver.
Human serum albumin binds ligands such as fatty acids and metals in circulation. Oxidative stress can modify albumin and affect ligand binding. This study examines the role of oxidative stress and fatty acids in modulating cobalt binding to albumin in patients with fatty liver. Elevated levels of malondialdehyde and protein carbonyls, indicative of oxidative stress were evident in serum of patients with fatty liver. A significant decrease in albumin-cobalt binding was also observed. Albumin isolated from patient serum also showed an increase in bound fatty acids. In vitro experiments indicated that while oxidant exposure or removal of fatty acids independently decreased cobalt binding to albumin, removal of fatty acids from the protein prior to oxidant exposure did not influence the oxidant effect on albumin-cobalt binding. These results suggest that oxidative stress and fatty acids on albumin can influence albumin-cobalt binding in patients with fatty liver by independent mechanisms.
Amirtharaj
G Jayakumar
GJ
The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Ida Scudder Road, Vellore-632004, India.
Natarajan
Sathish Kumar
SK
Mukhopadhya
Ashis
A
Zachariah
Uday George
UG
Hegde
Sudheer K
SK
Kurian
George
G
Balasubramanian
K A
KA
Ramachandran
Anup
A
D000328:Adult; D016022:Case-Control Studies; D003035:Cobalt / Q000378:metabolism*; D019327:Copper Sulfate / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D005234:Fatty Liver / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D005227:Fatty Acids; D012709:Serum Albumin; D019820:Xanthine; D003035:Cobalt; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D019327:Copper Sulfate