title: In vitro antiviral activity of plant extracts on goatpox virus replication.
abstract: Four plants having known medicinal properties were screened for inhibition of goatpox virus (GTPV) replication in vitro. Of the 4 plants, extract of Acacia arabica (Babul) and Eugenia jambolana (Jamun) leaves had inhibition (%) 99.70 and 99.92 at their maximum non toxic concentrations, 99.93 +/- 0.38 and 1999.73 +/- 0.50 microg/ml, respectively in all cytopathic effect (CPE) inhibition assays. Inhibition of GTPV virus replication was further confirmed by PCR and SYBR Green based quantitative real-time QPCR assays specific for GTPV. Results indicated that the extract of Acacia arabica and Eugenia jambolana leaves inhibited GTPV replication in vitro.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Mukteswar 263 138, India. bhanu6467@gmail.com
authors.forename: V
authors.initials: V
authors.lastname: Bhanuprakash
authors.forename: M
authors.initials: M
authors.lastname: Hosamani
authors.forename: V
authors.initials: V
authors.lastname: Balamurugan
authors.forename: P
authors.initials: P
authors.lastname: Gandhale
authors.forename: Ram
authors.initials: R
authors.lastname: Naresh
authors.forename: D
authors.initials: D
authors.lastname: Swarup
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000045:Acacia; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018151:Capripoxvirus / Q000187:drug effects*; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011213:Poxviridae Infections / Q000188:drug therapy*; D027842:Syzygium; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts