title: [Effect of arginine vasopressin on vascular reactivity and calcium sensitivity of vascular smooth muscle and its relationship to protein kinase C following hemorrhagic shock in rats].
abstract: OBJECTIVE
  To investigate the effect of arginine vasopressin (AVP) on vascular reactivity and calcium sensitivity following hemorrhagic shock and their relationship to protein kinase C (PKC) isoforms.
  
  
  METHODS
  With endothelium denuded superior mesenteric artery (SMA) rings procured from rats in hemorrhagic shock, the effects of AVP (5x10(-11), 5x10(-10) and 5x10(-9) mol/L) on contractile responses to norepinephrine (NE) and calcium sensitivity of SMA from hemorrhagic shock in rats and their relationship to alpha and Delta isoforms of PKC with isolated organ perfusion system were observed.
  
  
  RESULTS
  AVP (5x10(-11), 5x10(-10) and 5x10(-9) mol/L) markedly restored the vascular reactivity and calcium sensitivity following hemorrhagic shock, converting the cumulative concentration-response curve of NE and Ca2+ shift to the left, and its maximum concentration force (Emax) was significantly increased (all P<0.01) in a concentration dependent manner. Significant statistical differences were observed between the AVP groups (all P<0.05). Go6976 (5x10(-6) mol/L) and Rottlerin (10(-5) mol/L),respectively as the specific PKC alpha and PKC Delta isoforms inhibitor, antagonized AVP (5x10(-10) mol/L)-induced increase in vascular reactivity and calcium sensitivity of SMA following hemorrhagic shock and inhibited AVP-induced shift to the left of cumulative concentration-response curve of NE and Ca2+, and the Emax was significantly decreased (P<0.05 or P<0.01).
  
  
  CONCLUSIONS
  AVP significantly restored the decreased vascular reactivity and calcium sensitivity of vascular smooth muscle following hemorrhagic shock, and its underlying mechanisms may be related to both alpha and Delta isoforms of PKC activation.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Department 2, Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
authors.forename: Guang-ming
authors.initials: GM
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Jia
authors.initials: J
authors.lastname: Ming
authors.forename: Liang-ming
authors.initials: LM
authors.lastname: Liu
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D017538:Mesenteric Artery, Superior / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D009638:Norepinephrine / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000503:physiopathology*; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D001127:Arginine Vasopressin; D011493:Protein Kinase C; D002118:Calcium; D009638:Norepinephrine