Structural and functional characterization of osmotically inducible protein C (OsmC) from Thermococcus kodakaraensis KOD1.
Osmotically inducible protein C (OsmC) is involved in the cellular defense mechanism against oxidative stress caused by exposure to hyperoxides or elevated osmolarity. OsmC was identified by two-dimensional electrophoresis (2DE) analysis as a protein that is overexpressed in response to osmotic stress, but not under heat and oxidative stress. Here, an OsmC gene from T. kodakaraensis KOD1 was cloned and expressed in Escherichia coli. TkOsmC showed a homotetrameric structure based on gel filtration and electron microscopic analyses. TkOsmC has a significant peroxidase activity toward both organic and inorganic peroxides in high, but not in low temperature.
Park
Seong-Cheol
SC
Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, Jinju, 660-701, Republic of Korea.
Pham
Bang Phuong
BP
Van Duyet
Le
L
Jia
Baolei
B
Lee
Sangmin
S
Yu
Rui
R
Han
Sang Woo
SW
Yang
Jae-Kyung
JK
Hahm
Kyung-Soo
KS
Cheong
Gang-Won
GW
D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D020836:Protein Structure, Quaternary; D017386:Sequence Homology, Amino Acid; D019712:Thermococcus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D020122:tert-Butylhydroperoxide / Q000378:metabolism
D019843:Archaeal Proteins; D003432:Cross-Linking Reagents; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D009195:Peroxidase