Involvement of aldolase A in X-ray resistance of human HeLa and UV(r)-1 cells.
To find novel proteins involved in radio-resistance of human cells, we searched for nuclear proteins, whose expression levels alter after X-ray irradiation in HeLa cells, using agarose fluorescent two-dimensional differential gel electrophoresis following mass spectrometry. We identified 6 proteins, whose levels were increased in nuclei 24h after irradiation at 5Gy, including aldolase A. Nuclear aldolase A levels increased twofold after the irradiation, however, total aldolase A levels did not change. When the expression of aldolase A was suppressed by its specific siRNA, sensitization of the suppressed cells to X-ray-induced cell death was observed. In addition, UV(r)-1 cells with higher aldolase A expression exhibited lower sensitivity to X-ray-induced cell death than the parental RSa cells with lower aldolase A expression. These results suggest that aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol.
Lu
Jun
J
Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan.
Suzuki
Toshikazu
T
Satoh
Mamoru
M
Chen
Shiping
S
Tomonaga
Takeshi
T
Nomura
Fumio
F
Suzuki
Nobuo
N
D002467:Cell Nucleus / Q000201:enzymology / Q000528:radiation effects; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D040901:Proteomics; D034741:RNA, Small Interfering / Q000494:pharmacology; D011836:Radiation Tolerance / Q000187:drug effects / Q000235:genetics; D014965:X-Rays
D034741:RNA, Small Interfering; D005634:Fructose-Bisphosphate Aldolase