title: Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions.
abstract: Alloimmunization is a common phenomenon after transfusion, with an estimated incidence of 0.5% increasing to 20-60% in chronically transfused patients. In recently transfused patients, serological typing can be hampered by mixed field agglutination. We established RT-PCR methods for RHD, RHC/c and RHE/e typing using mRNA from reticulocytes. Molecular typing was performed soon after 51 separate mismatched transfusion events involving 30 patients. Accurate identification of the transfused patients' phenotype was confirmed in all cases. Reticulocyte maturation studies revealed that temperature is a crucial parameter for transition into mature red blood cells.
authors.affiliation: Department of Immunology and Transfusion Medicine, Ulleval University Hospital, Kirkeveien 166, 0407 Oslo, Norway. ingrid.randen@medisin.uio.no
authors.forename: Ingrid
authors.initials: I
authors.lastname: Randen
authors.forename: Kirsten
authors.initials: K
authors.lastname: Sørensen
authors.forename: Reidun
authors.initials: R
authors.lastname: Hauge
authors.forename: Anine B
authors.initials: AB
authors.lastname: Dahlberg
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Mirlashari
authors.forename: Keith M
authors.initials: KM
authors.lastname: Thompson
authors.forename: Jens
authors.initials: J
authors.lastname: Kjeldsen-Kragh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016477:Artifacts; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D027682:Cation Transport Proteins / Q000032:analysis / Q000235:genetics; D002470:Cell Survival; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D017707:Erythrocyte Transfusion; D004920:Erythropoiesis; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D047589:Leukocyte Reduction Procedures; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics; D008875:Middle Aged; D010641:Phenotype; D012333:RNA, Messenger / Q000097:blood* / Q000235:genetics; D012156:Reticulocytes / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012203:Rh Isoimmunization / Q000175:diagnosis*; D012204:Rh-Hr Blood-Group System / Q000032:analysis / Q000235:genetics*; D013997:Time Factors; D065227:Transfusion Reaction
chemical_list: D027682:Cation Transport Proteins; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; C066468:RHCE protein, human; C413213:RHCG protein, human; D012333:RNA, Messenger; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen