Correlation of autoantibodies presence detected by IFA-anti-dsDNA, IFA-AMA and immunoblotting with corresponding data in clinical management of autoimmune diseases.
Diagnosis and management of patients with SLE (Systemic Lupus Eritematosus), autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), involves specific diagnostic tests, such as IFA-AMA, IFA anti-dsDNA and immunoblotting for the detection of autoantibodies for specific autoantigens (mitochondria, dsDNA, M2, LKM-1, LC-1, SLA/LP). We established specific correlation between the detected autoantibodies and corresponding clinical findings. The total of 813 serum specimens were probed with IFA-anti-dsDNA, 98 of which tested positive. We also performed dilution analysis to the end point for all the positive specimens. Numerous specimens were tested by IFA, AMA and immunoblotting.
Subasić
Demo
D
Institute of Clinical Immunology - University of Sarajevo Clinics Centre, Bolnicka 25, 71000 Sarajevo, Bosnia and Herzegovina.
Karamehić
Jasenko
J
Ljuca
Farid
F
Gavrankapetanović
Faris
F
Delić-Sarac
Marina
M
Eminović
Izet
I
Kovacević
Dragan
D
D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D004247:DNA / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D019693:Hepatitis, Autoimmune / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology*; D032389:Myocytes, Smooth Muscle; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology
D001323:Autoantibodies; D011768:Pyruvate Dehydrogenase Complex; C060729:anti-liver kidney microsome antibody; D004247:DNA