title: Distinct H2O2 concentration promotes proliferation of tumour cells after transient oxygen/glucose deprivation.
abstract: A solid tumour undergoes ischemia/reperfusion due to deficient vascularization and subsequent formation of new blood vessels. This study investigated the effect of transient oxygen and glucose deprivation (OGD) on proliferation of C6 glioma cells. The cells were subjected to 18 h of OGD followed by reoxygenation in the presence of glucose and different extra-cellular H(2)O(2) concentrations since H(2)O(2) affects cell proliferation. After reoxygenation, the cellular H(2)O(2) concentration was increased returning to control levels within 24 h. Within this period, increase in cell number and MTT-reduction were impaired. Regeneration was completed on the third day of reoxygenation. MTT-reduction increased faster than cell number, indicating an OGD-dependent up-regulation of protein expression. It is concluded that ischemia/reperfusion stress promotes proliferation of tumour cells. An essential factor is a distinct H(2)O(2) concentration. Massive elevation as well as significant reduction of H(2)O(2) concentration impairs the proliferation process.
authors.affiliation: Institute of Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke-University, Magdeburg, Germany. lorenz.schild@med.ovgu.de
authors.forename: Lorenz
authors.initials: L
authors.lastname: Schild
authors.forename: Petr
authors.initials: P
authors.lastname: Makarow
authors.forename: Fahad
authors.initials: F
authors.lastname: Haroon
authors.forename: Karla
authors.initials: K
authors.lastname: Krautwald
authors.forename: Gerburg
authors.initials: G
authors.lastname: Keilhoff
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D008954:Models, Biological; D009369:Neoplasms / Q000473:pathology*; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D015427:Reperfusion Injury; D013778:Tetrazolium Salts / Q000737:chemistry / Q000494:pharmacology; D013844:Thiazoles / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000975:Antioxidants; D013778:Tetrazolium Salts; D013844:Thiazoles; D006861:Hydrogen Peroxide; C022616:thiazolyl blue; D005947:Glucose; D010100:Oxygen