[Studies on the methods of extract and determination of total tannins and gallic acids of the root of Euphorbia hylonoma].
OBJECTIVE
To determine total tannins and gallic acid of the root of Euphorbia hyloomla.


METHODS
With the gallic acid as reference, tannins and gallic acid of the root of Euphorbia hylonoma were determined by ultraviolet spectrophtometer and high performance liquid chromatography, respectively.


RESULTS
The content of tannins of the root of Euphorbia hylonoma were determined by different extract methods, including extracted by water, 70% alcohol, acetone on supersound, the determination data were 4.375%, 7.240%, 3.958%, respectively. When used recirculation method by water, 70% alcohol, acetone, the determination data were the determination data are 3.773%, 2.503%, 1.59%, respectively. The content of gallic acid of the root was 0.047%.


CONCLUSIONS
Content of total tannins by alcohol super sound is the highest comparing with other extract methods, this method can used in extract of total Tannins of the root of Euphorbia hylonoma.
Guo
Zeng-jun
ZJ
Faculty of Pharmacy of School of Medicine, Xian Jiao-tong University, Xihn 710061, China. guozj@mail.xjtu.edu.cn
Sun
Qi-shi
QS
Long
Li-hui
LH
Bu
Xiao-qian
XQ
D000096:Acetone; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D028481:Euphorbia / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013634:Tannins / Q000032:analysis* / Q000302:isolation & purification
D013634:Tannins; D000096:Acetone; D000431:Ethanol; D005707:Gallic Acid