[Discovery and identification of WU polyomavirus in children from Zhejing region].
WU polyomavirus, which was firstly discovered in 2007, is a new human polyomavirus belonging to Polyomaviridae and containing circular double-stranded genomic DNA. In this study, the 278 clinical sputum specimens from children under 5 years old were collected from Wenzhou Medical College affiliated Wenling First Hospital, Zhejiang Province. Based on identification assay of WU polyomavirus previously reported, a WU polyomavirus was identified from clinical samples successfully, the positive rate was 0.4%. The sequences of PCR products were identical to that of VP2 gene and large T antigen gene derived from WU polyomavirus reported. The above results strongly suggested that the WU polyomavirus isolated was firstly found in Chinese children with acute lower respiratory tract infections. This study provides a firm basis for further research of WU polyomavirus.
Lin
Feng
F
Wenzhou Medical College Affiliated Wenling Hospital, Wenling 317500, Zhejiang Province, China. linfengwyy@vip.sina.com
Zheng
Mei-Yun
MY
Li
Hua
H
Zheng
Chang-Hua
CH
Rao
Gao-Feng
GF
Zheng
Min-Qiao
MQ
Wu
Feng
F
Zeng
Ai-Ping
AP
Li
Xu-Yang
XY
Hou
Jian-Yi
JY
D001483:Base Sequence; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D013183:Sputum / Q000821:virology