title: Knockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigs.
abstract: BACKGROUND
  Xenotransplantation using porcine cells, tissues or organs may be associated with the transmission of porcine endogenous retroviruses (PERVs). More than 50 viral copies have been identified in the pig genome and three different subtypes of PERV were released from pig cells, two of them were able to infect human cells in vitro. RNA interference is a promising option to inhibit PERV transmission.
  
  
  METHODS
  We recently selected an efficient si (small interfering) RNA corresponding to a highly conserved region in the PERV DNA, which is able to inhibit expression of all PERV subtypes in PERV-infected human cells as well as in primary pig cells. Pig fibroblasts were transfected using a lentiviral vector expressing a corresponding sh (short hairpin) RNA and transgenic pigs were produced by somatic nuclear transfer cloning. Integration of the vector was proven by PCR, expression of shRNA and PERV was studied by in-solution hybridization analysis and real-time RT PCR, respectively.
  
  
  RESULTS
  All seven born piglets had integrated the transgene. Expression of the shRNA was found in all tissues investigated and PERV expression was significantly inhibited when compared with wild-type control animals.
  
  
  CONCLUSIONS
  This strategy may lead to animals compatible with PERV safe xenotransplantation.
authors.affiliation: Robert Koch Institute, Berlin, Germany.
authors.forename: Britta
authors.initials: B
authors.lastname: Dieckhoff
authors.forename: Björn
authors.initials: B
authors.lastname: Petersen
authors.forename: Wilfried A
authors.initials: WA
authors.lastname: Kues
authors.forename: Reinhard
authors.initials: R
authors.lastname: Kurth
authors.forename: Heiner
authors.initials: H
authors.lastname: Niemann
authors.forename: Joachim
authors.initials: J
authors.lastname: Denner
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D034741:RNA, Small Interfering