[Activation related genes of memory CD(4)(+) T cells in asthma patients].
OBJECTIVE
To screen and identify the genes of activated memory CD(4)(+) T cells in asthma.


METHODS
Differential display polymerase chain reaction (DDPCR) was utilized to identify genes of memory CD(4)(+) T cells after activation from asthmatic patients and normal individuals, and the mRNA levels of the differentially expressed genes were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Nineteen differentially expressed cDNA fragments were isolated. The homology analysis indicated that two fragments possessed a high degree of homology with interleukin-7 (IL-7) mRNA and MM-1 mRNA. RT-PCR confirmed that IL-7 mRNA and MM-1 mRNA levels were increased in memory CD(4)(+) T cells after activation in asthmatic patients (0.390 +/- 0.029, 0.629 +/- 0.047, F = 983, 1264 respectively, all P < 0.05).


CONCLUSIONS
The up-regulated expression of IL-7 and MM-1 genes in memory CD(4)(+) T cells after activation may be a molecular mechanism that differentiates the response to allergens of asthma patients from normal individuals.
Pi
Wei-Feng
WF
Department of Respiratory Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Guo
Xue-Jun
XJ
Xu
Xiang-Hui
XH
Ni
Pei-Hua
PH
Xu
Wei-Guo
WG
D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D015851:Interleukin-7 / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D015851:Interleukin-7; C115636:PFDN5 protein, human; D012333:RNA, Messenger; D012097:Repressor Proteins