Crystallization and preliminary X-ray study of native and selenomethionyl beta-1,4-mannanase AaManA from Alicyclobacillus acidocaldariusTc-12-31.
AaManA, a beta-1,4-mannanase from the thermoacidophile Alicyclobacillus acidocaldarius Tc-12-31, was expressed in Escherichia coli and purified in a form suitable for X-ray crystallographic analysis. Crystals were obtained using the hanging-drop vapour-diffusion method at 291 K using ammonium dihydrogen phosphate as a precipitant. Data were collected from native mannanase and from a selenomethionyl derivative to 1.90 and 1.99 A, respectively, at 100 K. The native crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.34, b = 75.55, c = 88.02 A. The derivative crystal belonged to the same space group as native AaManA, with unit-cell parameters a = 44.55, b = 75.70, c = 92.66 A.
Zhang
Yueling
Y
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Gao
Feng
F
Xue
Yanfen
Y
Zeng
Yan
Y
Peng
Hao
H
Qi
Jianxun
J
Ma
Yanhe
Y
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D003460:Crystallization; D008361:Mannosidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*; D014961:X-Ray Diffraction
D008715:Methionine; D008361:Mannosidases; D012643:Selenium