Normal birth from cryopreserved embryos after intracytoplasmic sperm injection of frozen semen into vitrified human oocytes.
OBJECTIVE
To describe the birth achieved from frozen embryos after intracytoplasmic sperm injection (ICSI) of donor sperm into vitrified oocytes.


METHODS
A 25-year-old woman whose husband was azoospermic undergoing IVF therapy.


METHODS
Oocytes collected after ovarian stimulation were vitrified, thawed, and fertilized by frozen donor sperm.


RESULTS
Nineteen oocytes were vitrified and all survived after thawing. Thirteen of the 19 oocytes that underwent ICSI with donors sperm were successfully fertilized. Twelve embryos were cryopreserved again by conventional slow-freezing protocol because of uterine bleeding on the day of transfer. Three thawed embryos were transferred, and a normal male with an infant karyotype of 46,XY was delivered.


CONCLUSIONS
This case report demonstrates effective oocyte cryopreservation by vitrification.
Chen
Gui An
GA
Department of Obstetrics & Gynecology, Third Hospital, Peking University, Peking, China. chenguian@bjmu.edu.cn
Cai
Xue Yong
XY
Lian
Ying
Y
Zheng
Xiao Ying
XY
Qiao
Jie
J
Chen
Xin Na
XN
Ye
Rong Hua
RH
D000328:Adult; D053713:Azoospermia / Q000628:therapy; D015925:Cryopreservation; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome; D012662:Semen Preservation; D020554:Sperm Injections, Intracytoplasmic; D014019:Tissue Donors