title: Expression of cell cycle associated proteins influences radiocurability of T2N0 squamous cell carcinoma of the larynx.
abstract: Cell cycle modulators are important in carcinogenesis and may be of prognostic and therapeutic relevance. This study has examined the influence of the proliferation index (Ki-67) and immunocytochemical expression of epidermal growth factor receptor (EGFR), cyclin D1, and retinoblastoma protein on recurrence rates at the primary site in 50 patients with T2N0 laryngeal carcinomas treated with radical irradiation. Pre-treatment biopsies were retrieved and sections scored for the four immunocytochemical markers. Statistical analysis for association, interaction and survival was performed. Five cases showed loss of expression of Rb protein. The median Ki-67 index was 50%, the median cyclin D1 index 21% and the median EGFR index 47% of cells. EGFR and cyclin D1 expression were positively correlated and, whilst local recurrence tended to occur with a Ki-67 labelling index of <50%, this was not statistically significant. When interactions were examined using Multiple Logistic Regression it was found that there was a direct relationship between EGFR and cyclin D1 expression. If the EGFR index was >20% and the cyclin D1 index >10%, then the odds ratio in favour of a primary site recurrence was very high (5.32 +/-0.41). This study demonstrates that the relationship between EGFR index and cyclin D1 index has a very strong association with primary site recurrence for T2 N0 laryngeal carcinomas treated by irradiation.
authors.affiliation: Division of Surgery and Oncology, University of Liverpool, Liverpool L69 3GA, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Rafferty
authors.forename: T R
authors.initials: TR
authors.lastname: Helliwell
authors.forename: D J
authors.initials: DJ
authors.lastname: Husband
authors.forename: J
authors.initials: J
authors.lastname: Fenton
authors.forename: T M
authors.initials: TM
authors.lastname: Jones
authors.forename: A S
authors.initials: AS
authors.lastname: Jones
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D016017:Odds Ratio; D016160:Retinoblastoma Protein / Q000378:metabolism*; D016019:Survival Analysis
chemical_list: D018797:Cell Cycle Proteins; D016160:Retinoblastoma Protein; D019938:Cyclin D1; D066246:ErbB Receptors