Molecular characterisation of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. isolates at a tertiary-care centre in Lebanon.
The prevalence of bla CTX-M, bla TEM and bla SHV genes among extended-spectrum beta-lactamase (ESBL)-producing clinical isolates of Escherichia coli (n = 50) and Klebsiella spp. (n = 50) from Lebanon was 96%, 57% and 67%, and 40%, 82% and 84%, respectively. Genotyping revealed that the clonal diversity was unrelated to the presence of bla genes. Sequence analysis of 16 selected isolates identified the bla CTX-M-15, bla TEM-1, bla OXA-1 and six bla SHV genes, as well as the gene encoding the quinolone-modifying enzyme AAC(6')-Ib-cr. The genes encoding CTX-M-15 and AAC(6')-Ib-cr were carried on a 90-kb plasmid of the pC15-1a or pCTX-15 type, which transferred both ESBL production and quinolone resistance from donors to transconjugants.
Kanj
S S
SS
Division of Infectious Diseases, American University of Beirut Medical Center, Beirut, Lebanon. sk11@aub.edu.lb
Corkill
J E
JE
Kanafani
Z A
ZA
Araj
G F
GF
Hart
C A
CA
Jaafar
R
R
Matar
G M
GM
D003428:Cross Infection / Q000382:microbiology; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D005838:Genotype; D006801:Humans; D007709:Klebsiella / Q000201:enzymology / Q000235:genetics*; D007861:Lebanon; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases