title: Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.
abstract: The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
authors.affiliation: Department of Pathology, Bacteriology, and Avian Diseases, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium. Hilde.Moyaert@UGent.be
authors.forename: H
authors.initials: H
authors.lastname: Moyaert
authors.forename: F
authors.initials: F
authors.lastname: Pasmans
authors.forename: R
authors.initials: R
authors.lastname: Ducatelle
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: M
authors.initials: M
authors.lastname: Baele
mesh_terms: D017931:DNA Primers / Q000235:genetics; D020459:Genes, rRNA; D016998:Helicobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S