title: Elevation of neuron-specific enolase and S-100beta protein level in experimental acute spinal cord injury.
abstract: We evaluated the protein levels of neuron-specific enolase (NSE) and S-100beta in serum and cerebrospinal fluid (CSF) in an animal model of acute spinal cord injury and ascertained their relevance. Spinal cord injury was induced at the T8 level in rats. Enzyme-linked immunosorbent assay was used to measure the protein levels of NSE and S-100beta in both serum and CSF at different time points (30 min, 2 h, 6 h, 12 h and 24 h after induction of spinal cord injury). There existed a significant correlation between neurological deficits and the severity of spinal cord injury (p<0.05). Compared with the control group, the protein levels of NSE and S-100beta in serum and CSF significantly increased from 2 h after injury (p<0.05) and reached a maximum at 6 h. Within a certain time window, the protein levels of NSE and S-100beta in serum and CSF were closely related to the severity of injury level (p<0.05). The protein levels of NSE and S-100beta in serum and CSF significantly increased after experimental spinal cord injury in a time-dependent manner and thus may be considered specific biomarkers for acute spinal cord injury.
authors.affiliation: Department of Neurosurgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Cao
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Yang
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Liu
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Hu
authors.forename: Gu
authors.initials: G
authors.lastname: Li
authors.forename: Xiu-jue
authors.initials: XJ
authors.lastname: Zheng
authors.forename: Fang
authors.initials: F
authors.lastname: Shen
authors.forename: Xue-qun
authors.initials: XQ
authors.lastname: Zhao
authors.forename: Shi-ting
authors.initials: ST
authors.lastname: Lv
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood* / Q000134:cerebrospinal fluid*; D009460:Neurologic Examination; D010751:Phosphopyruvate Hydratase / Q000097:blood* / Q000134:cerebrospinal fluid*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000097:blood* / Q000134:cerebrospinal fluid*; D012720:Severity of Illness Index; D013119:Spinal Cord Injuries / Q000097:blood* / Q000134:cerebrospinal fluid*; D013997:Time Factors
chemical_list: D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase