Synthesis of gelatin-containing PHBV nanofiber mats for biomedical application.
Electrospinning is one of the fabrication method to form ultra-fine fiber in a nano-scale made of synthetic and natural extracellular matrix components for tissue-engineering applications. In this study, a nanofibrous scaffold was obtained by co-electrospinning poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and gelatin in 2,2,2-trifluoroethanol (TFE) at a ratio of 50/50. The resulting fiber diameters were in the range of 400-1,000 nm without any beads. The nanofiber surfaces were characterized by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), electron spectroscopy for chemical analysis (ESCA), and atomic force microscopy. It was found, from cell culture experiments, that NIH 3T3 cells on the PHBV/gelatin nanofibrous scaffold more proliferated than on the PHBV nanofibrous scaffold.
Meng
Wan
W
Department of Polymer Science, Kyungpook National University, Daegu 702-701, Republic of Korea.
Xing
Zhi-Cai
ZC
Jung
Kyung-Hye
KH
Kim
Se-Yong
SY
Yuan
Jiang
J
Kang
Inn-Kyu
IK
Yoon
Sung Chul
SC
Shin
Hong In
HI
D000818:Animals; D005780:Gelatin / Q000032:analysis*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D049329:Nanostructures; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
D005780:Gelatin; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates