Use of glycogen phosphorylase BB measurement with POCT in the diagnosis of acute coronary syndromes. A comparison with the ELISA method.
BACKGROUND
Glycogen Phosphorylase BB (GPBB) is considered an early and specific marker of myocardial necrosis and ischemia. A POCT kit GPBB for diagnostic use has recently been approved.


OBJECTIVE
an evaluation of the correspondence of qualitative POCT GBPP measurements with ELISA test results.


METHODS
20 individuals with non-ST elevation myocardial infarction (non-STEMI) and 20 probands without acute coronary syndrome (ACS) were tested. GPBB (POCT, ELISA) in venous plasma (lithium-heparin) was assayed in all probands.


RESULTS
individuals with non-STEMI had significantly higher GPBB ELISA values (32.3 vs. 6.1 microg/l; p < 0.01). GPBB sensitivity and specificity for non-STEMI presence 6 hours after chest pain generation were 100 %. No proband was classified in a different subgroup with POCT of GPBB (positive/negative). GPBB POCT correlate with a non- STEMI diagnosis (chi(2) 36.1; p <0.01).


CONCLUSIONS
GPBB POCT measurement is comparable with ELISA test results. GPBB analysis could expand the diagnostic palette in the first hours after the onset of acute coronary syndrome.
Stejskal
David
D
Department of Laboratory Medicine, Sternberk Hospital, Czech Republic. david.stejskal@nemstbk.cz
Lacnak
Borek
B
Jedelsky
Libor
L
Stepanova
Leona
L
Proskova
Jitka
J
Solichova
Pavlina
P
Kadalova
Lenka
L
Janosova
Marie
M
Seitlova
Petra
P
Karpisek
Michal
M
Sprongl
Ludek
L
D054058:Acute Coronary Syndrome / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D024981:Glycogen Phosphorylase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D009203:Myocardial Infarction / Q000175:diagnosis*; D019095:Point-of-Care Systems; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D015415:Biomarkers; D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D024981:Glycogen Phosphorylase