[Effect of Qiangxin Fumai granule on myocyte apoptosis of sinus node and Bax, Bcl-2, Fas-L gene protein in rabbits of sinoatrial ischemia/reperfusion].
OBJECTIVE
To explore the molecular mechanism of Qiangxin Fumai granule (QFG, an effective Chinese composite drug) in preventing and treating sick sinus syndrome (SSS).


METHODS
Rabbit model of sinoatrial ischemia/reperfusion was established by occluding and loosening the root of right coronary artery. Effect of QFG on cell apoptosis was observed by TUNEL method, and its effect on apoptotic related gene Bax, Bcl-2 and Fas-L gene protein expression was observed by immunohistochemical method. Average light density values of the expression of Bax, Bcl-2 and Fas-L of SAN cells was determined by Imagepro Plus image analysis system.


RESULTS
Sinoatrial injury induced by ischemia/reperfusion could cause evident sinoatrial cell apoptosis, enhance Fas-L gene protein expression and obviously enhance Bax gene protein expression, reduce Bcl-2/Bax ratio. QFG could significantly down-regulate Fas-L and Bax gene protein expression, up-regulate Bcl-2/Bax ratio, significantly inhibit and block the sinoatrial cell apoptosis.


CONCLUSIONS
To inhibit and block the event of cell apoptosis through regulating Bax, Bcl-2 and Fas-L gene protein expression in sinoatrial node after ischemia/reperfusion might be one of the mechanisms of QFG in preventing and treating sinoatrial ischemia/reperfusion injury of SSS.
Liu
Ru-Xiu
RX
Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. liuruxiul@yahoo.com.cn
Tan
Shuang
S
Wu
Zhen-Yu
ZY
Zhang
Yun
Y
Huang
Xi
X
Liu
Zhi-Ming
ZM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D053222:Fas Ligand Protein / Q000378:metabolism*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D008856:Microscopy, Fluorescence; D032342:Muscle Cells / Q000166:cytology* / Q000187:drug effects*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D053222:Fas Ligand Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C532896:qiangxin fumai