title: Anti-histone antibodies in dogs with leishmaniasis and glomerulonephritis.
abstract: The association between serum anti-histone antibodies and glomerulonephritis was studied in 43 dogs with leishmaniasis (Leishmania infantum). Dogs with increased serum creatinine levels and urine protein-creatinine ratio >1 were considered to have glomerulonephritis. Moderately elevated anti-histone antibodies were found in 38.89% (7/18) of infected dogs without glomerulonephritis, whereas 88% of dogs with glomerulonephritis (22/25) showed moderate or strongly elevated anti-histone antibodies. Prevalence of positive anti-histone antibodies reactions and mean serum concentration was significantly higher (P<0.001; P<0.0001) in infected dogs with glomerulonephritis. Correlation between anti-histone antibodies and urine protein-creatinine ratio was significant when groups were analysed together (P<0.046). Positive predictive value for glomerulonephritis of positive anti-histone antibodies was 88%. In conclusion, high anti-histone antibodies are significantly associated with glomerulonephritis. Although other factors must be involved, dogs with moderate or strong positive anti-histone antibodies reactions may have a higher probability to develop glomerular lesions in canine leishmaniasis.
authors.affiliation: Department of Animal Medicine and Surgery, University of Córdoba, Campus de Rabanales, 14014 Córdoba, Spain. pginel@uco.es
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Ginel
authors.forename: Soledad
authors.initials: S
authors.lastname: Camacho
authors.forename: Rosario
authors.initials: R
authors.lastname: Lucena
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000627:therapeutic use*; D000918:Antibody Specificity; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004283:Dog Diseases / Q000188:drug therapy / Q000276:immunology* / Q000652:urine; D004285:Dogs; D005921:Glomerulonephritis / Q000188:drug therapy / Q000276:immunology / Q000652:urine / Q000662:veterinary*; D006657:Histones / Q000276:immunology*; D007891:Leishmania / Q000235:genetics / Q000302:isolation & purification; D007896:Leishmaniasis / Q000188:drug therapy / Q000276:immunology / Q000652:urine / Q000662:veterinary*; D008198:Lymph Nodes / Q000469:parasitology; D016133:Polymerase Chain Reaction
chemical_list: D000974:Antibodies, Antinuclear; D006657:Histones; D003404:Creatinine