title: A penetrating ocular injury can affect the induction of anterior chamber-associated immune deviation.
abstract: OBJECTIVE
  To determine the effect of penetrating ocular injury on the induction of anterior chamber-associated immune deviation (ACAID).
  
  
  METHODS
  An injection of 5 mul ovalbumin (OVA, 20 mg/ml) into the anterior chamber (AC) of female BALB/c mice was performed to induce ACAID. A penetrating ocular injury was induced via the limbus on OVA-inoculated eyes at 24 h, 48 h, 72 h, and 120 h following AC injection. The mice receiving an OVA inoculation without the ocular injury served as the AC-injection group. Delayed type hypersensitivity (DTH) was examined to evaluate the induction of ACAID. The levels of transforming growth factor (TGF)-beta1, interleukin (IL)-10, and interferon (IFN)-gamma produced by splenocytes were detected by enzyme-linked immunosorbent assays (ELISA). The frequency of CD4(+)CD25(+)Foxp3(+)T cells in the splenocytes was detected by flow cytometry.
  
  
  RESULTS
  A significantly decreased DTH response was observed in the AC-injection group as well as in mice that received a penetrating injury at 72 h and 120 h following AC-injection of OVA. The levels of TGF-beta1 and IL-10 produced by splenocytes of mice in the AC-injection group and in the 72-h and 120-h group were significant higher than those in the 24-h and 48-h group. However, the levels of IFN-gamma produced by splenocytes of the AC-injection group and the 72-h and 120-h group were significantly lower than those in the 24-h and 48-h group. An increased frequency of CD4(+)CD25(+)Foxp3(+)T cells was found in the AC-injection group and the 72-h and 120-h group.
  
  
  CONCLUSIONS
  Penetrating ocular injury preformed shortly (24 h-48 h) after an AC injection of an antigen was able to abrogate ACAID and was associated with a decreased production of TGF-beta1 and IL-10, an increased production of IFN-gamma, and a decreased expression of CD4(+)CD25(+)Foxp3(+)T cells.
authors.affiliation: Department of Ophthalmology, First Affiliated Hospital, Department of Microbiology and Immunology, Zheng Zhou University, Zhengzhou, Henan Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lei
authors.forename: Junfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Du
authors.forename: Peizeng
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects / Q000276:immunology* / Q000473:pathology*; D015704:CD4 Antigens / Q000378:metabolism; D005131:Eye Injuries / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D016850:Limbus Corneae / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D007371:Interferon-gamma; D010047:Ovalbumin