Patch clamp characterization of a non-selective cation channel of ER membranes purified from Beta vulgaris taproots.
The ER fraction from red beet taproot was purified on sucrose gradient and giant liposomes, suitable for patch clamping, were formed by dehydration-rehydration of the lipid film. Single-channel recordings on excised and attached patches revealed a large conductance (165 pS) cation (P(Cl-)/P(K+) < 0.03) channel with equal conductance and relative permeability for Na+ and K+. This non-selective cation channel was also highly permeable for Ca2+. We failed to detect any single-channel currents activated by a direct application of d-myo-inositol 1,4,5 trisphosphate, despite the fact that the ER membranes were native.
Brito-Argáez
Ligia
L
Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No 130, Chuburná de Hidalgo, Mérida 97200, Yucatán, México.
Canto-Canché
Blondy
B
Hernández-Sotomayor
Soledad Maria Teresa
SM
Martínez-Estevez
Manuel
M
Pottosin
Igor I
II
D027461:Beta vulgaris / Q000378:metabolism*; D002412:Cations; D004721:Endoplasmic Reticulum / Q000502:physiology*; D007473:Ion Channels / Q000302:isolation & purification / Q000502:physiology*; D008081:Liposomes; D018408:Patch-Clamp Techniques; D018517:Plant Roots / Q000378:metabolism*
D002412:Cations; D007473:Ion Channels; D008081:Liposomes