Exogenous hormones affecting morphology and biosynthetic potential of hairy root line (LYR2i) of Linum album.
A hairy root line of Linum album LYR2i was obtained via the genetic transformation of a cotyledon segment of the plant by cocultivating it with Agrobacterium rhizogenes and culturing it in hormone-free B5 Gamborg liquid medium. Characteristic changes in root morphology associated with variations in biosynthetic capabilities were achieved by adding different combinations of phytohormones to the basal medium. A reversible system between hairy roots and its cultures with a diversified morphology was established by including and excluding phytohormones in the basal medium. The medium containing indole 3 acetic acid (IAA) at a concentration of 3 mg/l (MI3) induced thicker root tips and that containing 2,4-dichlorophenoxy acetic acid (2,4-D) at a concentration of 1 mg/l (MD1) induced compact green callus. Podophyllotoxin and 6-methoxypodophyllotoxin content increased by 1.86-fold and 1.45-fold as a result of adding IAA (MI3) and 2,4-D (MD1) to the basal medium, respectively. After regeneration, the hairy roots regained their original morphology and biosynthetic capability on hormone-free basal medium. The transformation status of the regenerated hairy roots was confirmed by PCR analysis.
Farkya
Sunita
S
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Bisaria
Virendra Swarup
VS
D004305:Dose-Response Relationship, Drug; D019597:Flax / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000096:biosynthesis*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*
D010937:Plant Growth Regulators; D010940:Plant Proteins