Complete nucleotide sequence and organization of the mitogenome of the silk moth Caligula boisduvalii (Lepidoptera: Saturniidae) and comparison with other lepidopteran insects.
The 15,360-bp long complete mitogenome of Caligula boisduvalii possesses a gene arrangement and content identical to other completely sequenced lepidopteran mitogenomes, but different from the common arrangement found in most insect order, as the result of the movement of tRNA(Met) to a position 5'-upstream of tRNA Ile. The 330-bp A+T-rich region is apparently capable of forming a stem-and-loop structure, which harbors the conserved flanking sequences at both ends. Dissimilar to what has been seen in other sequenced lepidopteran insects, the initiation codon for C. boisduvalii COI appears to be TTG, which is a rare, but apparently possible initiation codon. The ATP8, ATP6, ND4L, and ND6 genes, which neighbor another PCG at their 3' end, all harbored potential sequences for the formation of a hairpin structure. This is suggestive of the importance of such structures for the precise cleavage of the mRNA of mature PCGs. Phylogenetic analyses of available sequenced species of Bombycoidea, Pyraloidea, and Tortricidea supported the morphology-based current hypothesis that Bombycoidea and Pyraloidea are monophyletic (Obtectomera). As previously suggested, Bombycidae (Bombyx mori and B. mandarina) and Saturniidae (Antheraea pernyi and C. boisduvalii) formed a reciprocal monophyletic group.
Hong
Mee Yeon
MY
College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
Lee
Eun Mee
EM
Jo
Yong Hun
YH
Park
Hae Chul
HC
Kim
Seong Ryul
SR
Hwang
Jae Sam
JS
Jin
Byung Rae
BR
Kang
Pil Don
PD
Kim
Ki-Gyoung
KG
Han
Yeon Soo
YS
Kim
Iksoo
I
D020856:AT Rich Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D017344:Genes, Insect; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D009036:Moths / Q000145:classification / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D003062:Codon; D004272:DNA, Mitochondrial; D019476:Insect Proteins