title: A new group of parasporal inclusions encoded by the S-layer gene of Bacillus thuringiensis.
abstract: Bacillus thuringiensis produces various groups of active proteins, such as Cyt, Vip and Parasporin, in addition to the Cry protein. In this study we show S-layer proteins to be a new group of parasporal inclusions of B. thuringiensis. The S-layer consists of a two-dimensional lattice structure and is the outermost component of many archaeobacteria and eubacteria. The parasporal inclusion of B. thuringiensis strain CTC was found to be not a typical crystal protein encoded by the cry gene, but a proteinaceous inclusion encoded by the S-layer gene. Furthermore, the CTC-like strains (with their parasporal inclusions coded by the S-layer gene) are widely distributed and accounted for 25.4% of the B. thuringiensis strains tested. These strains constitute a new group of parasporal inclusions encoded by the S-layer gene of B. thuringiensis and shed new light on B. thuringiensis nontoxic strains.
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhou
authors.initials: Z
authors.lastname: Zhou
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Ma
authors.forename: Jianping
authors.initials: J
authors.lastname: Liu
authors.forename: Chenguang
authors.initials: C
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D002479:Inclusion Bodies / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D007313:Insecta / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000254:growth & development
chemical_list: D004731:Endotoxins; D008562:Membrane Glycoproteins; C055764:S-layer proteins; C412914:parasporin