[Chemosensitivity test on brain tumors].
During last 50 years chemotherapy has played a very important part in the cancer treatment. However, success or failures of news drugs in one particular cancer its difficult to predict. In vitro chemosensitivity is an attractive method for knowing about responses of a tumor to ChT treatment and assess the best dose in the patient with cancer.


OBJECTIVE
To know brain tumors sensitivity against antineoplastic drugs.


METHODS
Five different drugs (carmustin, camptotecin, taxol, hydroxyurea and tamoxifen) were tested on short-term cultures from 7 patients with Glioblastoma multiforme, 15 patients with meningiomas and one patient with meduloblastoma. For testing chemosensitivity we used MTT assay, and we measured optic density by spectophotometry to 450 nm. RESULTS. A total of 49 measurement were done, getting 44 valid dose-result curves. For each drug we used from 10-2 M to 10-12 M gap, and IC50 result was representative of tumor sensitivity to the drug.


CONCLUSIONS
our data support MTT assays like valid method for measuring in vitro chemosensitivity in brain tumors to news drugs.
Gil-Salú
J L
JL
Servicios de Neurocirugía, Unidad Investigación, Hospital Universitario Puerta del Mar, Cádiz.
Bosco-López
J
J
Domi Nguez-Villar
M
M
Domínguez-Pascual
I
I
Pérez-Requena
J
J
Palomo
M J
MJ
López-Escobar
M
M
D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005562:Formazans / Q000378:metabolism; D006801:Humans; D013778:Tetrazolium Salts / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan