title: Comparative proteomics analysis of cerebrospinal fluid of patients with Guillain-Barré syndrome.
abstract: To better understand the pathophysiologic mechanisms underlying Guillain-Barré syndrome (GBS), Comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with GBS (the experiment group) and control subjects suffering from other neurological disorders (the control group) was carried out using two-dimensional gel electrophoresis (2-DE) technique, in combination with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and database searching to determine abnormal CSF proteins in GBS patients. Image analysis of 2-DE gels silver stained revealed that 10 protein spots showed significant differential expression between the two groups of CSF samples. The expression of cystatin C, transthyretin, apolipoprotein E and heat shock protein 70 were decreased. However, haptoglobin, alpha-1-antitrypsin, apolipoprotein A-IV and neurofilaments were elevated. The subsequent ELISA measured the concentration of cystatin C and confirmed the result of the proteomic analysis. These identified proteins may be involved in the pathophysiological process of GBS and call for further studying the role of these proteins in the pathogenesis of the disease.
authors.affiliation: Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University, 44 Wenhuaxi Road, Jinan, Shandong, P.R. China.
authors.forename: Yin-Rong
authors.initials: YR
authors.lastname: Yang
authors.forename: Shi-Lian
authors.initials: SL
authors.lastname: Liu
authors.forename: Zhao-Yu
authors.initials: ZY
authors.lastname: Qin
authors.forename: Fu-Jun
authors.initials: FJ
authors.lastname: Liu
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Qin
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Bai
authors.forename: Zhe-Yu
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D001054:Apolipoproteins A / Q000032:analysis / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000032:analysis / Q000134:cerebrospinal fluid; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020275:Guillain-Barre Syndrome / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000503:physiopathology; D006242:Haptoglobins / Q000032:analysis / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000032:analysis / Q000134:cerebrospinal fluid; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods*; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000134:cerebrospinal fluid
chemical_list: D001054:Apolipoproteins A; D015415:Biomarkers; D002556:Cerebrospinal Fluid Proteins; D006242:Haptoglobins; D016900:Neurofilament Proteins; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin; C022205:apolipoprotein A-IV