Langerhans cells are not required for efficient skin graft rejection.
The mechanism of skin allograft rejection has been thought to require presentation of graft antigen by resident epidermal Langerhans cells (LCs). We have previously engineered mice that have a selective and constitutive absence of epidermal LCs. By using donor skin from these LC-deficient mice, we show that LCs are not required for rejection of major (FVB --> B6) or minor (H-Y, male --> female on B6 background) antigen-mismatched skin grafts. On the FVB background, where H-Y mismatched grafts are normally maintained indefinitely, grafts lacking LCs are efficiently rejected. Thus, LCs in the donor graft are required for long-term skin engraftment, which supports a regulatory role for LCs in skin graft acceptance.
Obhrai
Jagdeep S
JS
Section of Nephrology, Yale University School of Medicine, New Haven, Connecticut, USA.
Oberbarnscheidt
Martin
M
Zhang
Na
N
Mueller
Daniel L
DL
Shlomchik
Warren D
WD
Lakkis
Fadi G
FG
Shlomchik
Mark J
MJ
Kaplan
Daniel H
DH
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism; D005260:Female; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D006182:H-Y Antigen / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000473:pathology; D007801:Langerhans Cells / Q000276:immunology* / Q000473:pathology; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D008297:Male; D037241:Mannose-Binding Lectins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D023421:Models, Animal; D012727:Sex Characteristics; D016038:Skin Transplantation / Q000276:immunology* / Q000473:pathology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology
D000954:Antigens, Surface; C498497:Cd207 protein, mouse; D006182:H-Y Antigen; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7