Follicle-stimulating hormone receptor polymorphism (Thr307Ala) is associated with variable ovarian response and ovarian hyperstimulation syndrome in Indian women.
OBJECTIVE
To evaluate the association of FSH receptor polymorphism and ovarian response.


METHODS
Retrospective study.


METHODS
Academic research institute and private IVF clinic.


METHODS
Fifty women were recruited in an assisted reproductive technology program (ART) and 100 proven fertile women of Indian origin.


METHODS
Polymerase chain reaction, restriction fragment-length polymorphism for detecting polymorphisms at T(307)A and N(680)S.


METHODS
FSH receptor polymorphisms, serum FSH, and estradiol levels, amount of FSH administered, occurrence of ovarian hyperstimulation syndrome (OHSS).


RESULTS
Prevalence of polymorphism at 307 position was 24%, 53%, and 23% in controls and 24%, 62%, and 14% in ART subjects for TT, TA, and AA, respectively, whereas at position 680, it was 31%, 56%, and 13% in controls and 42%, 46%, and 12% in ART subjects for NN, NS, and SS, respectively. The amount of FSH required for ovulation induction was low in AA compared with TT and TA subjects; the estradiol levels before and on the day of hCG administration were significantly higher. Eighty-five percent of the subjects with AA genotype developed OHSS.


CONCLUSIONS
In Indian women, the subjects with AA genotype require low amounts of FSH for ovarian stimulation and have an increased risk of developing OHSS.
Achrekar
Swati K
SK
National Institute for Research in Reproductive Health, Mumbai, India.
Modi
Deepak N
DN
Desai
Sadhana K
SK
Mangoli
Vijay S
VS
Mangoli
Ranjana V
RV
Mahale
Smita D
SD
D000328:Adult; D004252:DNA Mutational Analysis; D004958:Estradiol / Q000097:blood; D005260:Female; D005300:Fertility Agents, Female / Q000009:adverse effects*; D043373:Follicle Stimulating Hormone, Human / Q000009:adverse effects* / Q000097:blood; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007194:India; D016017:Odds Ratio; D016471:Ovarian Hyperstimulation Syndrome / Q000097:blood / Q000139:chemically induced / Q000208:ethnology / Q000235:genetics*; D010060:Ovulation / Q000208:ethnology / Q000235:genetics*; D010062:Ovulation Induction / Q000009:adverse effects*; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011962:Receptors, FSH / Q000235:genetics*; D012189:Retrospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D044465:White People / Q000235:genetics*
D005300:Fertility Agents, Female; D043373:Follicle Stimulating Hormone, Human; D011962:Receptors, FSH; D004958:Estradiol