Low-level laser therapy decreases levels of lung neutrophils anti-apoptotic factors by a NF-kappaB dependent mechanism.
OBJECTIVE
Low-level laser therapy (LLLT) is a known modulator of inflammatory process. Herein we studied the effect of 660 nm diode laser on mRNA levels of neutrophils anti-apoptotic factors in lipopolysaccharide (LPS)-induced lung inflammation. STUDY DESIGN/METHODOLOGY: Mice were divided into 8 groups (n=7 for each group) and irradiated with energy dosage of 7.5 J/cm(2). The Bcl-xL and A1 mRNA levels in neutrophils were evaluated by Real Time-PCR (RT-PCR). The animals were irradiated after exposure time of LPS.


RESULTS
LLLT and an inhibitor of NF-kappaB nuclear translocation (BMS 205820) attenuated the mRNA levels of Bcl-xL and A1 mRNA in lung neutrophils obtained from mice subjected to LPS-induced inflammation.


CONCLUSIONS
LLLT reduced the levels of anti-apoptotic factors in LPS inflamed mice lung neutrophils by an action mechanism in which the NF-kappaB seems to be involved.
Aimbire
F
F
Institute of Research and Development-IP&D-UNIVAP. Av. Shishima Hifumi, 2911, CEP: 12244-000, São José dos Campos-SP, Brazil. aimbire@univap.br
Santos
F V
FV
Albertini
R
R
Castro-Faria-Neto
H C
HC
Mittmann
J
J
Pacheco-Soares
C
C
D000818:Animals; D007249:Inflammation; D008070:Lipopolysaccharides / Q000276:immunology; D028022:Low-Level Light Therapy; D008168:Lung / Q000276:immunology* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism* / Q000528:radiation effects; D010455:Peptides / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism / Q000528:radiation effects; D051020:bcl-X Protein / Q000378:metabolism*
C096816:BCL2-related protein A1; C411484:BMS 205820; C494812:Bcl2l1 protein, mouse; D008070:Lipopolysaccharides; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051020:bcl-X Protein