Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection.
Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.
Mukherjee
Shankar
S
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Nagajyothi
Fnu
F
Mukhopadhyay
Aparna
A
Machado
Fabiana S
FS
Belbin
Thomas J
TJ
Campos de Carvalho
Antonio
A
Guan
Fangxia
F
Albanese
Chris
C
Jelicks
Linda A
LA
Lisanti
Michael P
MP
Silva
Joao S
JS
Spray
David C
DC
Weiss
Louis M
LM
Tanowitz
Herbert B
HB
D000818:Animals; D002598:Chagas Cardiomyopathy / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014349:Trypanosoma cruzi / Q000502:physiology