title: Multicomponent blood analysis by near-infrared Raman spectroscopy.
abstract: We demonstrate the use of Raman spectroscopy to measure the concentration of many important constituents (analytes) in serum and whole blood samples at physiological concentration in vitro across a multipatient data set. A near-infrared (830-nm) diode laser generates Raman spectra that contain superpositions of Raman signals from different analytes. Calibrations for glucose, cholesterol, urea, and other analytes are developed by use of partial least-squares cross validation. We predict six analytes in serum with significant accuracy in a 66-patient data set, using 60-s spectra. The calibrations are shown to be fairly robust against system drift over the span of seven weeks. In whole blood, a preliminary analysis yields accurate predictions of some of the same analytes and also hematocrit. The results hold promise for potential medical applications.
authors.affiliation: GR Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. ajberger@bli.edu
authors.forename: A J
authors.initials: AJ
authors.lastname: Berger
authors.forename: T W
authors.initials: TW
authors.lastname: Koo
authors.forename: I
authors.initials: I
authors.lastname: Itzkan
authors.forename: G
authors.initials: G
authors.lastname: Horowitz
authors.forename: M S
authors.initials: MS
authors.lastname: Feld