title: Isolation of discrete nanoparticle-DNA conjugates for plasmonic applications.
abstract: Discrete DNA-gold nanoparticle conjugates with DNA lengths as short as 15 bases for both 5 and 20 nm gold particles have been purified by anion-exchange HPLC. Conjugates comprising short DNA (<40 bases) and large gold particles (> or =20 nm) are difficult to purify by other means and are potential substrates for plasmon coupling experiments. Conjugate purity is demonstrated by hybridizing complementary conjugates to form discrete structures, which are visualized by TEM.
authors.affiliation: Department of Chemistry, Berkeley, CA 94720, USA.
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Claridge
authors.forename: Huiyang W
authors.initials: HW
authors.lastname: Liang
authors.forename: S Roger
authors.initials: SR
authors.lastname: Basu
authors.forename: Jean M J
authors.initials: JM
authors.lastname: Fréchet
authors.forename: A Paul
authors.initials: AP
authors.lastname: Alivisatos
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles
chemical_list: D004247:DNA