title: Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis.
abstract: A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.
authors.affiliation: Department of Biomedical Engineering, University of California, 451 Health Sciences Drive, Davis, California, 95616, USA.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Sun
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Elson
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Hollars
authors.forename: Javier A
authors.initials: JA
authors.lastname: Jo
authors.forename: Jesung
authors.initials: J
authors.lastname: Park
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Laura
authors.initials: L
authors.lastname: Marcu
mesh_terms: D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D005454:Fluorescence Polarization; D006801:Humans; D007834:Lasers; D000432:Methanol / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D009991:Oscillometry; D012031:Refractometry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
chemical_list: D000431:Ethanol; D000432:Methanol