[Study on effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats].
OBJECTIVE
To build an experimental method to determine the content of puerarin in plasma, observe the effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats suffering cerebral ischemia attack after being orally administered the main active parts of Yangyin Tongnao granules.


METHODS
Absorption of puerarin in plasma was extracted by acetonitrile. Using hydroxybenzoate as interior standard, methanol-0.5% acetic acid water solution as mobile phase, ODS C(18) chromatography column (4.6 mm x 150 mm, 5 microm) as stationary phase, puerarin was detected at 248 nm.


RESULTS
There is a good linear relationship for puerarin in the range 0.025-3.2 microg x mL-1, RSD < 10%, and the average recovery is 97.6%.


CONCLUSIONS
The method is sensitive, reliable, and can be well applied in this experiment. It can provide a method for determination of active components in TCM preparation in vitro. Different compatibility influences metabolism of components.
Guo
Ying
Y
Institute of Heart Brain Vessel Disease, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Wan
Hai-Tong
HT
Zhang
Li
L
Wang
Yu
Y
Han
Jin
J
Yu
Qin
Q
Pan
Yuan-Jiang
YJ
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D019540:Area Under Curve; D002545:Brain Ischemia / Q000097:blood; D004338:Drug Combinations; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification; D007529:Isoflavones / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification
D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D047309:Flavones; D007529:Isoflavones; D009822:Oils, Volatile; D012503:Saponins; C033607:puerarin