Xylitol production from rice straw hemicellulose hydrolyzate by polyacrylic hydrogel thin films with immobilized Candida subtropicalis WF79.
Xylose from rice straw hemicellulose hydrolysate was fermented for xylitol production using Candida subtropicalis WF79 cells immobilized in polyacrylic hydrogel thin films of 200 mum thickness. Cell immobilization was conducted by first suspending the yeast cells in a mixture of 2-hydroxyethyl methacrylate (HEMA, hydrophilic monomer), polyethylene glycol diacrylate (PEG-DA, crosslinking agent), and benzoin isopropyl ether (photoinitiator). The mixture was then allowed to form polyacrylic hydrogel thin films, between two pieces of glass sheets, by UV-initiated photopolymerization. The hemicellulose of rice straw was hydrolyzed using dilute sulfuric acid at 126 degrees C. The hydrolysate was neutralized with calcium hydroxide. After separating the solid residues and calcium sulfate precipitates by filtration, the hydrolysate was treated with charcoal to partially remove potential inhibitory substances, followed by vacuum concentration to obtain solutions of desired xylose concentrations for yeast fermentation. The thin films with immobilized yeast cells were submerged in the xylose solution from rice straw hydrolysate for fermentation in an Erlenmeyer flask. The maximum yield was 0.73 g of xylitol per gram of xylose consumed. In the 52.5-day long durability test, after 40 d of repeated batchwise operation, the fermentation activities of the cell immobilized in thin films began to decline to a yield of 0.57 g/g at the end.
Liaw
Wen-Chang
WC
Department of Chemical Engineering, National Yunlin University of Science and Technology, 123, Section 3, University Road, Touliu, Yunlin 640, Taiwan, ROC. liawwc@yuntech.edu.tw
Chen
Chee-Shan
CS
Chang
Wen-Shion
WS
Chen
Kuan-Pin
KP
D000180:Acrylic Resins / Q000737:chemistry*; D041022:Candida tropicalis / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized; D005782:Gels / Q000737:chemistry; D006868:Hydrolysis; D012275:Oryza / Q000382:microbiology*; D035261:Plant Components, Aerial / Q000382:microbiology*; D011134:Polysaccharides / Q000378:metabolism*; D014993:Xylitol / Q000378:metabolism*
D000180:Acrylic Resins; D005782:Gels; D011134:Polysaccharides; C006903:carbopol 940; C007916:hemicellulose; D014993:Xylitol