Molecular recognition of small-cell lung cancer cells using aptamers.
Early diagnosis is the way to improve the rate of lung cancer survival, but is almost impossible today due to the lack of molecular probes that recognize lung cancer cells sensitively and selectively. We developed a new aptamer approach for the recognition of specific small-cell lung cancer (SCLC) cell-surface molecular markers. Our approach relies on cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) to evolve aptamers for whole live cells that express a variety of surface markers representing molecular differences among cancer cells. When applied to different lung cancer cells including those from patient samples, these aptamers bind to SCLC cells with high affinity and specificity in various assay formats. When conjugated with magnetic and fluorescent nanoparticles, the aptamer nanoconjugates could effectively extract SCLC cells from mixed cell media for isolation, enrichment, and sensitive detection. These studies demonstrate the potential of the aptamer approach for early lung cancer detection.
Chen
Hui William
HW
Shands Cancer Center, Department of Chemistry, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
Medley
Colin D
CD
Sefah
Kwame
K
Shangguan
Dihua
D
Tang
Zhiwen
Z
Meng
Ling
L
Smith
Josh E
JE
Tan
Weihong
W
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000032:analysis; D018288:Carcinoma, Small Cell / Q000737:chemistry / Q000175:diagnosis*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D015342:DNA Probes / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry / Q000175:diagnosis*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D052156:SELEX Aptamer Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured
D052157:Aptamers, Nucleotide; D014408:Biomarkers, Tumor; D015342:DNA Probes; D004247:DNA