Comparing whole genomes using DNA microarrays.
The rapid accumulation of complete genomic sequences offers the opportunity to carry out an analysis of inter- and intra-individual genome variation within a species on a routine basis. Sequencing whole genomes requires resources that are currently beyond those of a single laboratory and therefore it is not a practical approach for resequencing hundreds of individual genomes. DNA microarrays present an alternative way to study differences between closely related genomes. Advances in microarray-based approaches have enabled the main forms of genomic variation (amplifications, deletions, insertions, rearrangements and base-pair changes) to be detected using techniques that are readily performed in individual laboratories using simple experimental approaches.
Gresham
David
D
Lewis-Sigler Institute for Integrative Genomics, Department of Molecular Biology, Carl Icahn Laboratory, Princeton University, Princeton, New Jersey 08544, USA. dgresham@genomics.princeton.edu
Dunham
Maitreya J
MJ
Botstein
David
D
D000818:Animals; D002874:Chromosome Mapping; D018628:Gene Dosage; D014644:Genetic Variation; D016678:Genome; D023281:Genomics / Q000379:methods / Q000706:statistics & numerical data; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000706:statistics & numerical data; D020641:Polymorphism, Single Nucleotide