title: Characteristics of the phagocytic cup induced by uropathogenic Escherichia coli.
abstract: Uropathogenic Escherichia coli invade the urothelial umbrella cells by using the zipper mechanism. However, the details of the early events of this invasion, such as the formation of the phagocytic cup, are not yet well understood. We show here, using thin section electron microscopy and immunogold labeling, that the plasma membrane curves around the bacterial surface in the phagocytic cup. There exists a uniform gap between the bacterium and the urothelial membrane, and actin filaments are present in the phagocytic cup. We suggest that the action-reaction between the mechanical forces generated by pilus retraction of the bacterium and the actin polymerization in the urothelial cell plays a role in maintaining the phagocytic cup. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
authors.affiliation: Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA.
authors.forename: Huaibin
authors.initials: H
authors.lastname: Wang
authors.forename: Feng-Xia
authors.initials: FX
authors.lastname: Liang
authors.forename: Xiang-Peng
authors.initials: XP
authors.lastname: Kong
mesh_terms: D000199:Actins / Q000502:physiology; D000818:Animals; D004926:Escherichia coli / Q000502:physiology*; D005260:Female; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051379:Mice; D008809:Mice, Inbred C3H; D046529:Microscopy, Electron, Transmission; D010587:Phagocytosis; D001743:Urinary Bladder / Q000382:microbiology* / Q000648:ultrastructure; D019459:Urothelium / Q000382:microbiology* / Q000648:ultrastructure
chemical_list: D000199:Actins