Immunotoxicity evaluation of jet a jet fuel in female rats after 28-day dermal exposure.
The potential for jet fuel to modulate immune functions has been reported in mice following dermal, inhalation, and oral routes of exposure; however, a functional evaluation of the immune system in rats following jet fuel exposure has not been conducted. In this study potential effects of commercial jet fuel (Jet A) on the rat immune system were assessed using a battery of functional assays developed to screen potential immunotoxic compounds. Jet A was applied to the unoccluded skin of 6- to 7-wk-old female Crl:CD (SD)IGS BR rats at doses of 165, 330, or 495 mg/kg/d for 28 d. Mineral oil was used as a vehicle to mitigate irritation resulting from repeated exposure to jet fuel. Cyclophosphamide and anti-asialo GM1 were used as positive controls for immunotoxic effects. In contrast to reported immunotoxic effects of jet fuel in mice, dermal exposure of rats to Jet A did not result in alterations in spleen or thymus weights, splenic lymphocyte subpopulations, immunoglobulin (Ig) M antibody-forming cell response to the T-dependent antigen, sheep red blood cells (sRBC), spleen cell proliferative response to anti-CD3 antibody, or natural killer (NK) cell activity. In each of the immunotoxicological assays conducted, the positive control produced the expected results, demonstrating the assay was capable of detecting an effect if one had occurred. Based on the immunological parameters evaluated under the experimental conditions of the study, Jet A did not adversely affect immune responses of female rats. It remains to be determined whether the observed difference between this study and some other studies reflects a difference in the immunological response of rats and mice or is the result of other factors.
Mann
Cynthia M
CM
ExxonMobil Biomedical Sciences, Inc., Annandale, New Jersey 08801-0971, USA.
Peachee
Vanessa L
VL
Trimmer
Gary W
GW
Lee
Ji-Eun
JE
Twerdok
Lorraine E
LE
White
Kimber L
KL
D000818:Animals; D005260:Female; D006838:Hydrocarbons / Q000633:toxicity*; D007107:Immune System / Q000187:drug effects*; D000922:Immunotoxins / Q000633:toxicity*; D051381:Rats; D012869:Skin Absorption; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology; D018675:Toxicity Tests
D006838:Hydrocarbons; D000922:Immunotoxins; C029662:JP5 jet fuel