Effects of high hydrostatic pressure on the growth and beta-carotene production of Rhodotorula glutinis.
The effects of high hydrostatic pressure (HHP) on the biomass and beta-carotene biosynthesis of Rhodotorula glutinis R68 were studied. After treatment with five repeated cycles at 300 MPa for 15 min, the barotolerant mutant PR68 was obtained. After 72 h of culture, the biomass of mutant PR68 was 21.6 g/l, decreased by 8.5% compared to the parental strain R68, but its beta-carotene production reached 19.4 mg/l, increased by 52.8% compared to the parental strain R68. The result of restriction fragment length polymorphism (RFLP) analysis suggested that mutant strain PR68 was likely to change in nucleic acid level, and thus enhanced beta-carotene production in this strain as a result of gene mutation induced by HHP treatment.
Wang
Sui-Lou
SL
Department of Food Science and Safety, China Pharmaceutical University, Nanjing 210009, Jiangsu, People's Republic of China. cpuwsl@126.com
Chen
De-Jing
DJ
Deng
Bai-Wan
BW
Wu
Xiao-Zong
XZ
D018533:Biomass; D002851:Chromatography, High Pressure Liquid; D006874:Hydrostatic Pressure; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D012248:Rhodotorula / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D019207:beta Carotene / Q000032:analysis / Q000378:metabolism*
D019207:beta Carotene