Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.
The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.
Moyaert
H
H
Department of Pathology, Bacteriology, and Avian Diseases, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium. Hilde.Moyaert@UGent.be
Pasmans
F
F
Ducatelle
R
R
Haesebrouck
F
F
Baele
M
M
D017931:DNA Primers / Q000235:genetics; D020459:Genes, rRNA; D016998:Helicobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S