title: Immunocytochemical typing of primary tumors on fine-needle aspiration cytologies of lymph nodes.
abstract: The aim of this study was to analyze the role of immunocytochemistry as an ancillary method on routine FNACs of enlarged lymph nodes, using different markers. In a validating cohort study all patients had confirmatory histological and/or clinical follow-up. 10 FNACs were analyzed for the differentiation of Non-Hodgkin Lymphoma (NHL) from metastatic carcinoma (MC), 30 cases to identify the sites of metastatic unknown primary tumors and 16 cases were checked to confirm clinical suspicion of a specific MC. Accuracy to differentiate NHL from MC was 100%, 92.3% to identify a primary tumor site of MC, and 100% to confirm a clinical suspicion of a specific MC. In 7 cases, the site of the primary tumor remained clinically unknown. Application of immunocytochemical markers on the same slide used for microscopic diagnosis is a useful tool in the routine assessment of FNACs of lymph nodes.
authors.affiliation: Institute of Cytopathology, Heinrich-Heine University, Duesseldorf, Germany.
authors.forename: Alexandre Sherlley
authors.initials: AS
authors.lastname: Casimiro Onofre
authors.forename: Natalia
authors.initials: N
authors.lastname: Pomjanski
authors.forename: Birgit
authors.initials: B
authors.lastname: Buckstegge
authors.forename: Alfred
authors.initials: A
authors.lastname: Böcking
mesh_terms: D000465:Algorithms; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor / Q000032:analysis; D044963:Biopsy, Fine-Needle; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000145:classification / Q000473:pathology; D008297:Male; D009369:Neoplasms / Q000145:classification* / Q000473:pathology*; D015203:Reproducibility of Results
chemical_list: D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor