title: Stimuli-responsive hydrogel-silver nanoparticles composite for development of localized surface plasmon resonance-based optical biosensor.
abstract: In this paper, the development of a localized surface plasmon resonance (LSPR)-based optical enzyme biosensor using stimuli-responsive hydrogel-silver nanoparticles composite is described. This optical enzyme biosensor was constructed by immobilizing glucose oxidase (GOx) into the stimuli-responsive hydrogel. When a sample solution such as glucose was applied to the surface of this optical enzyme biosensor, the interparticle distances of the silver nanoparticles present in the stimuli-responsive hydrogel were increased, and thus the absorbance strength of the LSPR was decreased. Furthermore, hydrogen peroxide, which was produced by the enzymatic reaction, induced the degradation of highly clustered silver nanoparticles by the decomposition of hydrogen peroxide. Hence, a drastic LSPR absorbance change, which depends on the glucose concentrations, could be observed. On the basis of the abovementioned mechanism, the characterization of the LSPR-based optical enzyme biosensor was carried out. It was found that the LSPR-based optical enzyme biosensor could be used to specifically determine glucose concentrations. Furthermore, the detection limit of this biosensor was 10 pM. Therefore, this LSPR-based optical enzyme biosensor has the potential to be applied in cost-effective, highly simplified, and highly sensitive test kits for medical applications.
authors.affiliation: Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan. endo.t.ab@m.titech.ac.jp
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Endo
authors.forename: Ryuzoh
authors.initials: R
authors.lastname: Ikeda
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Yanagida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hatsuzawa
mesh_terms: D015374:Biosensing Techniques; D005947:Glucose / Q000032:analysis; D020100:Hydrogels; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D020100:Hydrogels; D012834:Silver; D006861:Hydrogen Peroxide; D005947:Glucose