title: Nonclassical secretion of human catalase on the surface of CHO cells is more efficient than classical secretion.
abstract: There is a great demand for improved production of therapeutic proteins using mammalian cell expression systems and transgenic animals. There have been intensive endeavors to optimize production at the transcriptional and translational levels, but comparatively little attention has been paid to the secretory level, especially to nonclassical secretion. To compare the efficiencies of classical and nonclassical secretion, we expressed GFP-tagged human catalase conjugated with a classical signal peptide and with several short peptides derived from mouse Engrailed 2 (mEN2) homeoprotein for nonclassical secretion and internalization in CHO cells. Surprisingly, the results showed that the secretory efficiency was significantly greater (up to 2.3 fold) than classical secretion when the fusion protein was driven by the secretory sequence (SS) of mEN2, and up to 1.9 fold when the classical secretion process was modified by incorporating the internalization sequence (IS) of mEN2. The effect of these short peptides on nonclassical secretion and internalization may indicate potential applications in the improved production of complex therapeutic proteins in mammalian cell expression systems and transgenic animals.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, PR China.
authors.forename: Zuyong
authors.initials: Z
authors.lastname: He
authors.forename: Xiuzhu
authors.initials: X
authors.lastname: Sun
authors.forename: Gui
authors.initials: G
authors.lastname: Mei
authors.forename: Shengli
authors.initials: S
authors.lastname: Yu
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002374:Catalase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D014176:Protein Biosynthesis; D021381:Protein Transport; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D002374:Catalase