title: Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma.
abstract: Circulating nucleic acids have been shown to have potential as non-invasive diagnostic markers in cancer. We therefore investigated whether microRNAs also have diagnostic utility by comparing levels of tumour-associated MIRN155 (miR-155), MIRN210 (miR-210) and MIRN21 (miR-21) in serum from diffuse large B-cell lymphoma (DLBCL) patients (n = 60) with healthy controls (n = 43). Levels were higher in patient than control sera (P = 0.009, 0.02 and 0.04 respectively). Moreover, high MIRN21 expression was associated with relapse-free survival (P = 0.05). This is the first description of circulating microRNAs and suggests that microRNAs have potential as non-invasive diagnostic markers for DLBCL and possibly other cancers.
authors.affiliation: Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK. charles.lawrie@ndcls.ox.ac.uk
authors.forename: Charles H
authors.initials: CH
authors.lastname: Lawrie
authors.forename: Shira
authors.initials: S
authors.lastname: Gal
authors.forename: Heather M
authors.initials: HM
authors.lastname: Dunlop
authors.forename: Beena
authors.initials: B
authors.lastname: Pushkaran
authors.forename: Amanda P
authors.initials: AP
authors.lastname: Liggins
authors.forename: Karen
authors.initials: K
authors.lastname: Pulford
authors.forename: Alison H
authors.initials: AH
authors.lastname: Banham
authors.forename: Francesco
authors.initials: F
authors.lastname: Pezzella
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Boultwood
authors.forename: James S
authors.initials: JS
authors.lastname: Wainscoat
authors.forename: Christian S R
authors.initials: CS
authors.lastname: Hatton
authors.forename: Adrian L
authors.initials: AL
authors.lastname: Harris
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012334:RNA, Neoplasm / Q000097:blood*; D012189:Retrospective Studies; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012334:RNA, Neoplasm