Significant association of interleukin 10 receptor mRNA levels with renal cell carcinoma metastasis.
Immunosuppressive cytokine, interleukin 10 (IL-10), is associated with progression of the renal cell carcinoma (RCC). However, the roles of its cell surface receptor, interleukin 10 receptor (IL-10R), remain elusive. We quantified IL-10R mRNA expression in paired tumor and non-tumor samples from the surgical specimens of 71 consecutive patients with RCC using a real-time reverse transcription polymerase chain reaction (RT-PCR). The absolute level of IL-10R mRNAs in tumor and non-tumor tissues did not correlate with the malignant and metastatic profiles. The relative yields of the PCR product from the tumor tissue to that from the corresponding non-tumor tissue (T/N) for the expression of IL-10R mRNAs were calculated. A high T/N ratio of IL-10R correlated with poor differentiation (P < 0.001) and metastasis (P < 0.0001). By univariate analysis, a high T/N ratio of IL-10R predicted a shortened overall survival in all cases (P < 0.01). These findings suggest that IL-10R is associated with the progression of RCC.
Abe
Hideyuki
H
Department of Urology, Dokkyo Medical University, Tochigi, Japan.
Yamanishi
Tomonori
T
Mashidori
Tomoko
T
Arai
Kyoko
K
Kamai
Takao
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000175:diagnosis / Q000235:genetics; D009362:Neoplasm Metastasis / Q000175:diagnosis / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis; D053701:Receptors, Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015996:Survival Rate
D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D053701:Receptors, Interleukin-10