Short- and long-term immunogenicity and protection induced by non-replicating smallpox vaccine candidates in mice and comparison with the traditional 1st generation vaccine.
This study assessed three non-replicating smallpox vaccine candidates (modified vaccinia Ankara (MVA), NYVAC and HR) for their immunogenicity and ability to protect mice against an intranasal cowpox virus challenge and compared them with the traditional replicating vaccine. A single immunisation with the non-replicating vaccines induced a complete protection from death at short-term, but was not fully protective when mice were challenged 150 days post-vaccination with protection correlated with the specific neutralizing antibodies and CD4(+) T-cells responses. Prime-boost vaccination enabled effective long-term protection from death for mice vaccinated with MVA, but protection from disease and CD4(+) T-cell level were lower than the ones induced by the traditional vaccine over the long-term period. Further investigations are necessary with MVA to determine the optimal conditions of immunisation to induce at long-term immunogenicity and protection observed with the 1st generation smallpox vaccine.
Ferrier-Rembert
Audrey
A
Unité de Virologie, Centre de Recherches du Service de Santé des Armées Emile Pardé, F-38702 Grenoble, France.
Drillien
Robert
R
Tournier
Jean-Nicolas
JN
Garin
Daniel
D
Crance
Jean-Marc
JM
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D015608:Cowpox virus / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000378:metabolism; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D007117:Immunization, Secondary; D007150:Immunohistochemistry; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012899:Smallpox / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control*; D012900:Smallpox Vaccine / Q000009:adverse effects / Q000276:immunology*; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000009:adverse effects / Q000276:immunology; D012901:Variola virus / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
D016207:Cytokines; D012900:Smallpox Vaccine; D014613:Vaccines, Attenuated