Determination of antihypertensive peptides from an izumi shrimp hydrolysate.
The izumi shrimp (Plesionika izumiae Omori, 1971) is an unused resource which can be caught off the southern coast of Tokushima Prefecture. We have previously found that an izumi shrimp hydrolysate significantly inhibited the age-associated spontaneous increase in blood pressure in stroke-prone spontaneously hypertensive rats. In this present study, two angiotensin I-converting enzyme inhibitory peptides were isolated from an izumi shrimp hydrolysate by using high-performance liquid chromatography, and their amino acid sequences were determined to be Val-Trp-Tyr-His-Thr and Val-Trp. A single oral administration of synthetic Val-Trp-Tyr-His-Thr or Val-Trp significantly decreased the blood pressure in stroke-prone spontaneously hypertensive rats. The antigenicity and allergenicity of the izumi shrimp hydrolysate against BALB/c mice were very low. These results demonstrate that the angiotensin I-converting enzyme inhibitory peptides isolated from the izumi shrimp hydrolysate had an anti-hypertensive effect on rats.
Nii
Yoshitaka
Y
Food Technology Division, Tokushima Prefectural Industrial Technology Center, Tokushima 770-8021, Japan. nii@itc.pref.tokushima.jp
Fukuta
Kazuhiro
K
Yoshimoto
Ryoko
R
Sakai
Kentaro
K
Ogawa
Tadashi
T
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004342:Drug Hypersensitivity; D006973:Hypertension / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D033561:Penaeidae; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides