Comparative glycomics of connective tissue glycosaminoglycans.
Homeostasis of connective joint tissues depends on the maintenance of an extracellular matrix, consisting of an integrated assembly of collagens, glycoproteins, proteoglycans, and glycosaminoglycans (GAGs). Isomeric chondroitin sulfate (CS) glycoforms differing in position and degree of sulfation and uronic acid epimerization play specific and distinct functional roles during development and disease onset. This work profiles the CS epitopes expressed by different joint tissues as a function of age and osteoarthritis. GAGs were extracted from joint tissues (cartilage, tendon, ligment, muscle, and synovium) and partially depolymerized using chondroitinase enzymes. The oligosaccharide products were differentially stable isotope labeled by reductive amination using 2-anthranilic acid-d(0) or -d(4) and subjected to amide-hydrophilic interaction chromatography (HILIC) online LC-MS/MS. The analysis presented herein enables simultaneous profiling of the expression of nonreducing end, linker region, and Delta-unsaturated interior oligosaccharide domains of the CS chains among the different joint tissues. The results provide important new information on the changes to the expression of CS GAG chains during disease and development.
Hitchcock
Alicia M
AM
Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA.
Yates
Karen E
KE
Costello
Catherine E
CE
Zaia
Joseph
J
D000368:Aged; D000375:Aging; D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry; D002417:Cattle; D002809:Chondroitin Sulfates / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D019295:Computational Biology; D003238:Connective Tissue / Q000737:chemistry*; D005260:Female; D054794:Glycomics; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D008022:Ligaments / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000737:chemistry; D013583:Synovial Membrane / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods; D013710:Tendons / Q000737:chemistry
D006025:Glycosaminoglycans; D002809:Chondroitin Sulfates