High immunogenicity of a hydrophilic component of the hepatitis B virus preS1 sequence exposed on the surface of three virus-like particle carriers.
The preS1phil, a hydrophilic component of the hepatitis B virus (HBV) preS1 sequence, was exposed on the surface of three widely used virus-like particle (VLP) carriers by (i) insertion into the HI loop of the murine polyomavirus (MPyV) VP1, (ii) N-terminal addition to the hepatitis B surface (HBs) protein, and (iii) insertion into the major immunodominant region (MIR) of three hepatitis B core (HBc) vectors with different structure of their C-termini. Adjuvant-free immunisation of Balb/c mice demonstrated high preS1-specific antibody responses, but strong Th1 cell activation with efficient induction of IgG2a isotype antibodies was observed only in those VLPs, and namely in two of three HBc derivatives, which contained packaged RNA.
Skrastina
Dace
D
Latvian Biomedical Research and Study Centre, Ratsupites 1, LV-1067 Riga, Latvia.
Bulavaite
Aiste
A
Sominskaya
Irina
I
Kovalevska
Larisa
L
Ose
Velta
V
Priede
Dace
D
Pumpens
Paul
P
Sasnauskas
Kestutis
K
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D006509:Hepatitis B / Q000276:immunology*; D006512:Hepatitis B Core Antigens / Q000235:genetics / Q000378:metabolism; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007279:Injections, Subcutaneous; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D011120:Polyomavirus / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
D000914:Antibodies, Viral; D036022:Capsid Proteins; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007074:Immunoglobulin G; D011498:Protein Precursors; C062481:VP1 protein, polyomavirus; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen