Evidence for insulin suppression of baseline luteinizing hormone in women with polycystic ovarian syndrome and normal women.
BACKGROUND
In women with polycystic ovarian syndrome (PCOS), the relationship of insulin to LH secretion and responses to GnRH remains unresolved. A rigorous analytical examination of this relationship has not been performed.


OBJECTIVE
Our objective was to determine the relationship of basal LH secretion and responses to GnRH, insulin, and other endocrine variables in normal and PCOS women.


METHODS
In PCOS and normal women, mean composite 12-h LH secretion was analyzed for correlating factors. LH responses to varying doses of GnRH during a fixed rate of insulin infusion and LH responses to a fixed dose of GnRH during varying doses of insulin infusion were analyzed for contributing factors.


METHODS
Eighteen PCOS and 21 normal women underwent studies of frequent blood sampling and GnRH stimulation before and during insulin infusion at the General Clinical Research Center, University of California, San Diego.


METHODS
Group mean composite 12-h LH levels were assessed with respect to other endocrine variables. In addition, LH responses to GnRH with or without insulin infusion were assessed.


RESULTS
In normal women, insulin negatively predicted mean LH. In PCOS, the combined effect of body mass index (negative) and testosterone (positive) predicted LH. The best predictor of LH was body mass index and insulin combined. Basal LH and LH responses to GnRH were unaltered by insulin infusion in normal women. These measures were reduced during insulin infusion in PCOS women.


CONCLUSIONS
In PCOS, insulin infusion suppresses pituitary response to GnRH. In normal women, insulin negatively correlates with mean LH and suppresses GnRH response at a high infusion rate.
Lawson
Mark A
MA
Department of Reproductive Medicine 0674, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0674. mlawson@ucsd.edu
Jain
Sonia
S
Sun
Shelly
S
Patel
Ketan
K
Malcolm
Pamela J
PJ
Chang
R Jeffrey
RJ
D000328:Adult; D001481:Basal Metabolism / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015309:Glucose Clamp Technique; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage; D006801:Humans; D007262:Infusions, Intravenous; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism*; D015999:Multivariate Analysis; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*
D007328:Insulin; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone