Prevention of assay interference in infectious-disease serology tests done on the liaison platform.
Immunoassay interference causing unexpected reactive results in magnetic-microparticle-based assays was detected. A systematic evaluation of Liaison Epstein-Barr virus immunoglobulin M showed that 5% of the positive results (0.4% of tested samples) could be explained by such interference. Adding chemical blocking reagents (polyvinylpyrrolidone and polyvinyl alcohol) to the assay buffers partially prevented this phenomenon.
Berth
Mario
M
Algemeen Medisch Laboratorium, Immunology Department, Desguinlei 88, 2018 Antwerpen, Belgium. mario.berth@aml-lab.be
Bosmans
Eugene
E
D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D001681:Biological Assay; D019411:Clinical Laboratory Techniques; D005189:False Positive Reactions; D004854:Herpesvirus 4, Human / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D007075:Immunoglobulin M / Q000097:blood*; D012698:Serologic Tests
D000914:Antibodies, Viral; D007075:Immunoglobulin M