title: Functional Fe3O4/TiO2 core/shell magnetic nanoparticles as photokilling agents for pathogenic bacteria.
abstract: A photokilling approach for pathogenic bacteria is demonstrated using a new type of magnetic nanoprobe as the photokilling agent. In addition to their magnetic property, the nanoprobes have other features including a photocatalytic property and the capacity to target bacteria. The nanoprobes comprise iron oxide/titania (Fe(3)O(4)@TiO(2)) core/shell magnetic nanoparticles. As dopamine molecules can self-assemble onto the surface of the titania substrate, dopamine is used as the linker to immobilize succinic anhydride onto the surfaces of the Fe(3)O(4)@TiO(2) nanoparticles. This is followed by the immobilization of IgG via amide bonding. We demonstrate that the IgG-Fe(3)O(4)@TiO(2) magnetic nanoparticles not only have the capacity to target several pathogenic bacteria, but they also can effectively inhibit the cell growth of the bacteria targeted by the nanoparticles under irradiation of a low-power UV lamp within a short period. Staphylococcus saprophyticus, Streptococcus pyogenes, and antibiotic-resistant bacterial strains, such as multiantibiotic-resistant S. pyogenes and methicillin-resistant Staphylococcus aureus (MRSA), are used to demonstrate the feasibility of this approach.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Chen
authors.forename: Pei-Jane
authors.initials: PJ
authors.lastname: Tsai
authors.forename: Yu-Chie
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D001419:Bacteria / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D005290:Ferric Compounds / Q000737:chemistry* / Q000528:radiation effects; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D010777:Photochemistry / Q000379:methods; D013242:Sterilization / Q000379:methods*; D014025:Titanium / Q000737:chemistry* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D005290:Ferric Compounds; C009495:titanium dioxide; C000499:ferric oxide; D014025:Titanium