[The experimental investigation of the invasion and growth of laryngeal cancer by matrix metalloproteinase-2 and matrix metalloproteinase-9 gene silence together].
OBJECTIVE
To investigate the inhibitive role of the invasion and growth of laryngeal cancer by lentivirus mediated Matrix Metalloproteinase-2 (MMP-2) and Matrix Metalloproteinase-2 (MMP-9) gene silence.


METHODS
RNA interference (RNAi) technic was used. Recombinant lentivirus of small interference RNA targeting MMP-2 and MMP-9 gene were transduced into Hep-2 cells, and Reverse transcriptase polymerase chain reaction (RT-PCR) was used to test MMP-2 and MMP-9 expression in Hep-2 cells. The effect of the invasive capability in Hep-2 cells after MMP-2-RNAi-lentivirus and MMP-9-RNAi-lentivirus transduction was observed by Boyden assay. In vivo experiment, the nude mouse model of laryngeal squamous carcinoma was established and MMP-2-RNAi-lentivirus and MMP-9-RNAi-lentivirus were intratumoral injected. Finally, the tumor inhibitive effect was observed and the Proliferating cell nuclear antigen (PCNA) expression in xenografts were examined to evaluate proliferation change of the Hep-2 cells.


RESULTS
The recombinant lentivirus can transduce the target cells efficiently. RT-PCR showed the expression of MMP-2 and MMP-9 were negative. Boyden assay showed there were (14 +/- 4) Hep-2 cells permeate artificial basement which was less than that (32 +/- 6) in the control group. In vivo, the average tumor weight and volume in treated group were significantly lower than those in the control group (P < 0.01) and the tumor inhibitive rate was 46.59%. Immunohistochemistry showed PCNA index in treated group was obviously lower than that in control group (P < 0.01).


CONCLUSIONS
The invasion, growth and proliferation of laryngeal cancer can be inhibited by lentivirus mediated MMP-2 and MMP-9 gene silence together.
Sun
Ya-Nan
YN
Department of Otorhinolaryngology, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Yang
Bao-Feng
BF
Liu
Ming
M
Guo
Yan-Ling
YL
Tian
Lin-Li
LL
Jiao
Hui
H
D000818:Animals; D045744:Cell Line, Tumor; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016086:Lentivirus / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014183:Transplantation, Heterologous; D047368:Tumor Burden
D018809:Proliferating Cell Nuclear Antigen; D034741:RNA, Small Interfering; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9