[Study of antitumous effect of the complex prescription of Chinese crude drug in athymic mice with HCC].
OBJECTIVE
To research the treatment effect of a complex prescription of Chinese crude drug in BALB/c athymic mice.


METHODS
48 male BALB/c athymic mouse models were built by Bel-7402 with an indirect method. After 24 hours of postoperation, they were distributed randomly into 4 groups which were treated by intragastric administration with complex prescription of Chinese crude drug of different density as the low, middle, high and FF207 (Tegafur) for 4 weeks. At last, athymic mice were put to death and survial time, volume of tumors, exponent of tumors and the tumor metastasis in livers were observed; and PTEN was detected in hepatic tissue, latero-cancer tissue and cancer tissue by immunohistochemistry.


RESULTS
4 weeks later, the total survival rate in treatment group (A + B + C) was 50% and more higher than the control group (0%) treated by FT207, P < 0.01. The survival rate in group A, B, C was higher than group D, except group A, there was significant differences, P < 0.05 or 0.01. And no differences were observed between the treatment groups and the control group in volume of tumors and exponent of tumors, P > 0.05. Tumor metastasis in livers of the treatment group is less than the control's, P < 0.05. Negative correlation between volume of tumors and survival time, positive correlation between tumors volume and spleen exponent were observed, P < 0.01. The result of immunohistochemistry represented that the intensity of PTEN in latero-cancer tissue was the highest, and then the hepatic tissue, the lowest was cancer tissue, P < 0.01. It also represented that the intensity of PTEN in treatment groups (A, B, C) was more higher than the control group (D), and group B was the highest in the treatment groups, P < 0.05 or P < 0.01. However, there was no significant statistic difference between group A and group C, P > 0.05.


CONCLUSIONS
This complex prescription of Chinese crude drug can prolong the survival time and decrease tumor metastasis in livers of these experimental mice. Too large tumor volume is an main death cause of model. Mechanisms of this complex prescription of Chinese crude drug healing HCC may partially be explained by enhancing the expression of PTEN in liver.
Sun
Bao-guo
BG
First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Chen
Ze-xiong
ZX
Zhang
Shi-jun
SJ
Liu
Yong-dong
YD
Huang
Hong-zhong
HZ
Yin
Li-rong
LR
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000737:chemistry; D016019:Survival Analysis; D023041:Xenograft Model Antitumor Assays
D000972:Antineoplastic Agents, Phytogenic; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D051059:PTEN Phosphohydrolase