Th1 and Th2 in human IVF pregnancy with allogenic fetus.
OBJECTIVE
Positive obstetric outcome of allogenic in vitro fertilization (IVF) pregnancies makes interesting the subject of additional regulatory mechanisms after oocyte donation.


METHODS
Eighty eight women: 23 donation of oocytes (DO), 33 IVF, 32 natural conception (NC) were studied in first trimester of pregnancy. Intracellular production of cytokines and chemokine receptors expression were studied by flow cytometry.


RESULTS
Intracellular production of interferon-gamma (IFN-gamma), interleukin (IL)-4, tumor necrosis factor-alpha by CD4 T lymphocytes in DO women was higher than in IVF and NC women. Ratio IFN-gamma/IL-4 in DO was lower than in IVF. We found higher expression of chemokine receptor CCR4 but not CXCR3 on CD4 T cells in DO compared with IVF and NC. Ratio CXCR3/CCR4 in DO was lower than in NC.


CONCLUSIONS
Hyperactivation of T helper 1 (Th1) and T helper 2 (Th2) by allogenic fetus is specific for DO pregnancy. Preferable activation of Th2 and relative suppression of Th1 chemokine expression reflect additional regulatory counteractive mechanism(s).
Chernyshov
Victor P
VP
Laboratory of Immunology, Institute of Pediatrics, Obstetrics and Gynecology, Academy of Medical Sciences, Kiev, Ukraine. chernyshov@ukr.net
Tumanova
Larisa E
LE
Sudoma
Irina A
IA
Bannikov
Vladimir I
VI
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005333:Fetus / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D018587:Oocyte Donation; D011247:Pregnancy; D054367:Receptors, CXCR3 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
D016207:Cytokines; D054367:Receptors, CXCR3; D019718:Receptors, CXCR4