Resonance Raman spectroscopy can detect structural changes in haemozoin (malaria pigment) following incubation with chloroquine in infected erythrocytes.
Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ are observed. The intensity changes are attributed to intermolecular drug binding of the CQ in a sandwich type complex between ferriprotoporphyrin IX (FePPIX) dimer units. It is postulated that the CQ binds via pi-pi interactions between adjacent and orientated porphyrins thereby disrupting the haemozoin aggregate and reducing excitonic interactions between adjacent haems. The results show the potential of Raman microscopy as a screening tool for FePPIX:drug interactions in live cells.
Webster
Grant T
GT
Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Vic. 3800, Australia.
Tilley
Leann
L
Deed
Samantha
S
McNaughton
Don
D
Wood
Bayden R
BR
D000818:Animals; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D002738:Chloroquine / Q000737:chemistry / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000469:parasitology*; D006420:Hemeproteins / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry; D006801:Humans; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D013059:Spectrum Analysis, Raman; D053058:Trophozoites / Q000187:drug effects
D000962:Antimalarials; D006420:Hemeproteins; C020753:hemozoin; D006427:Hemin; D002738:Chloroquine