From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens.
Mannheimia succiniciproducens is a capnophilic gram-negative bacterium isolated from bovine rumen. Wild-type M. succiniciproducens can produce succinic acid as a major fermentation product with acetic, formic, and lactic acids as byproducts during the anaerobic cultivation using several different carbon sources. Succinic acid is an important C4 building block chemical for many applications. Here, we review the progress made with M. succiniciproducens for efficient succinic acid production; the approaches taken towards the development of an integrated process for succinic acid production are described, which include strain isolation and characterization, complete genome sequencing and annotation, development of genetic tools for metabolic engineering, strain development by systems approach of integrating omics and in silico metabolic analysis, and development of fermentation and recovery processes. We also describe our current effort on further improving the performance of M. succiniciproducens and optimizing the mid- and downstream processes. Finally, we finish this mini-review by discussing the issues that need to be addressed to make this process of fermentative succinic acid production employing M. succiniciproducens to reach the industrial-scale process.
Lee
Sang Yup
SY
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Daejeon, Republic of Korea. leesy@kaist.ac.kr
Kim
Ji Mahn
JM
Song
Hyohak
H
Lee
Jeong Wook
JW
Kim
Tae Yong
TY
Jang
Yu-Sin
YS
D002952:Citric Acid Cycle; D003470:Culture Media; D005285:Fermentation; D020868:Gene Silencing; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D016680:Genome, Bacterial; D031121:Mannheimia / Q000235:genetics* / Q000378:metabolism*; D008954:Models, Biological; D010957:Plasmids; D040901:Proteomics; D019802:Succinic Acid / Q000302:isolation & purification* / Q000378:metabolism*
D003470:Culture Media; D019802:Succinic Acid