Identification by suppression subtractive hybridization of genes expressed in pear (Pyrus spp.) upon infestation with Cacopsylla pyri (Homoptera: Psyllidae).
The molecular interaction between pear tree (Pyrus spp.) and the phloem-feeding psylla Cacopsylla pyri (Linnaeus) was investigated through the construction and characterization of cDNA subtracted libraries. Genes expressed upon insect infestation were identified in the susceptible pear cultivar Bartlett and in the resistant selection NY10355. In both interactions, genes involved in the plant defense response were induced, confirming the observed similarity between the response to pathogens and to insects with piercing/sucking mouthparts. However, the two expression profiles were found to be different, with more genes involved in the response to biotic and abiotic stress being activated in the resistant plant than in the susceptible one. Further characterization of the identified genes could lead to the development of molecular markers associated with tolerance/resistance to pear psylla.
Salvianti
Francesca
F
Dipartimento di Biologia Animale e Genetica Leo Pardi, Università degli Studi di Firenze, via Romana 17-19, 50121 Firenze, Italy.
Bettini
Priscilla P
PP
Giordani
Edgardo
E
Sacchetti
Patrizia
P
Bellini
Elvio
E
Buiatti
Marcello
M
D000818:Animals; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D005838:Genotype; D006430:Hemiptera / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D031989:Pyrus / Q000235:genetics* / Q000469:parasitology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger