title: IL-4-induced Stat6 activities affect apoptosis and gene expression in breast cancer cells.
abstract: IL-4-induced Stat6 signaling is active in a variety of cell types, including immune cells and cancer cells, and plays an important role in the regulation of gene expression. Using EMSA gel shift assay and an antibody to Stat6, we phenotyped two breast cancer cell lines, ZR-75-1 being active Stat6(high) phenotype and BT-20 being defective Stat6(null) phenotype, respectively. Breast cancer cells carrying Stat6(null) phenotype exhibited increased spontaneous apoptosis compared with those carrying Stat6(high) phenotype. Expression microarray analyses demonstrated that IL-4 upregulated CCL26, SOCS1, CISH, EGLN3, and SIDT1, and downregulated DUSP1, FOS, and FOSB, respectively, in these breast cancer cells. Among those genes, CCL26 and SOCS1 were known genes regulated by IL-4/Stat6 pathway, but CISH, EGLN3, SIDT1, DUSP1, FOS, and FOSB were novel genes demonstrated to be IL-4 responsive for the first time. IL-4 also upregulated 38 genes unique to Stat6(null) BT-20 cells and 23 genes unique to Stat6(high) ZR-75-1 cells, respectively. Furthermore, Stat6(high) and Stat6(null) cells showed very different profiles of constitutively expressed genes relevant to apoptosis and metastasis among others, which serve as a valuable expression database and warrant for detailed studies of IL-4/Stat6 pathway in breast cancer.
authors.affiliation: Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, Hubei 430030, China. zhangwj82@yahoo.com
authors.forename: Wen Jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Ben Hui
authors.initials: BH
authors.lastname: Li
authors.forename: Xian Zi
authors.initials: XZ
authors.lastname: Yang
authors.forename: Pin Dong
authors.initials: PD
authors.lastname: Li
authors.forename: Qin
authors.initials: Q
authors.lastname: Yuan
authors.forename: Xiao Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Shuang Bing
authors.initials: SB
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Yuan
authors.forename: Glenn S
authors.initials: GS
authors.lastname: Gerhard
authors.forename: Kathryn K
authors.initials: KK
authors.lastname: Masker
authors.forename: Cheng
authors.initials: C
authors.lastname: Dong
authors.forename: Walter A
authors.initials: WA
authors.lastname: Koltun
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Chorney
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D015203:Reproducibility of Results; D050800:STAT6 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; D015847:Interleukin-4