title: Purification, crystallization and preliminary X-ray analysis of the peptidoglycan N-acetylglucosamine deacetylase BC1960 from Bacillus cereus in the presence of its substrate (GlcNAc)6.
abstract: The peptidoglycan N-acetylglucosamine (GlcNAc) deacetylase BC1960 from Bacillus cereus (EC 3.5.1.33), an enzyme consisting of 275 amino acids, was crystallized in the presence of its substrate (GlcNAc)(6). The crystals belonged to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 92.7, c = 242.9 A and four molecules in the asymmetric unit. A complete data set was collected at 100 K to a resolution of 2.38 A using synchrotron radiation.
authors.affiliation: Department of Biology, University of Crete, PO Box 2208, GR-71110, Heraklion, Crete, Greece.
authors.forename: Aleka
authors.initials: A
authors.lastname: Tsalafouta
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Psylinakis
authors.forename: Evangelia G
authors.initials: EG
authors.lastname: Kapetaniou
authors.forename: Dina
authors.initials: D
authors.lastname: Kotsifaki
authors.forename: Alexandra
authors.initials: A
authors.lastname: Deli
authors.forename: Alexandros
authors.initials: A
authors.lastname: Roidis
authors.forename: Vasilis
authors.initials: V
authors.lastname: Bouriotis
authors.forename: Michael
authors.initials: M
authors.lastname: Kokkinidis
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry* / Q000378:metabolism*; D000581:Amidohydrolases / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001409:Bacillus cereus / Q000201:enzymology* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D013379:Substrate Specificity
chemical_list: D000581:Amidohydrolases; C023079:N-acetylglucosamine deacetylase; D000117:Acetylglucosamine