The peripheral chromosome scaffold, a novel structural component of mitotic chromosomes.
Using an original high-salt extraction protocol, we observed a novel chromosome substructure, referred to as the peripheral chromosome scaffold. This chromosome domain contained the perichromosomal layer proteins pKi-67, B23/nucleophosmin and fibrillarin, but no DNA fragments (i.e., the loop domain bases were not associated with the peripheral scaffold). Modern models of chromosome organization do not predict the existence of a peripheral chromosome scaffold domain, and thus our observations have conceptual implications for understanding chromosome architecture.
Sheval
Eugene V
EV
Department of Electron Microscopy, AN Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russia. sheval_e@genebee.msu.su
Polyakov
Vladimir Y
VY
D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D002877:Chromosomes, Human / Q000737:chemistry / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000235:genetics*
D002868:Chromosomal Proteins, Non-Histone