title: The distribution of 4-hydroxynonenal-modified proteins in gastric mucosa of duodenal peptic ulcer patients.
abstract: This study used monoclonal antibody specific for 4-hydroxynonenal (HNE)-histidine to evaluate immunohistochemical distribution of HNE-protein adducts in gastric mucosa biopsies of 52 peptic ulcer patients (all positive for H. pylori) and of 20 healthy volunteers (eight positive and 12 negative for H. pylori). HNE-modified proteins were present in glandular epithelium in all subjects, both patients with duodenal peptic ulcer and healthy subjects. Hence, the presence of HNE did not appear to be related to the presence of H. pylori. However, in patients with duodenal peptic ulcer accumulation of HNE-protein adducts was frequently observed also in nuclei, while in the control group such subcellular distribution of HNE was not observed at all. This study shows physiological presence of HNE in human gastric mucosa, but also suggests its role in pathology of gastric dysfunction in duodenal peptic ulcer patients manifested by accumulation of HNE-protein adducts in particular in nuclei of gastric glandular epithelium.
authors.affiliation: Department of Histology, Cytology & Embryology, Lviv National Medical University, Lviv, Ukraine.
authors.forename: Olha
authors.initials: O
authors.lastname: Yelisyeyeva
authors.forename: Andriy
authors.initials: A
authors.lastname: Cherkas
authors.forename: Kamelija
authors.initials: K
authors.lastname: Zarkovic
authors.forename: Khrystyna
authors.initials: K
authors.lastname: Semen
authors.forename: Danylo
authors.initials: D
authors.lastname: Kaminskyy
authors.forename: Georg
authors.initials: G
authors.lastname: Waeg
authors.forename: Neven
authors.initials: N
authors.lastname: Zarkovic
mesh_terms: D000293:Adolescent; D000328:Adult; D000447:Aldehydes / Q000737:chemistry*; D003593:Cytoplasm / Q000378:metabolism; D004381:Duodenal Ulcer / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000473:pathology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010437:Peptic Ulcer / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology
chemical_list: D000447:Aldehydes; C027576:4-hydroxy-2-nonenal