Efficiency of propolis extract against mitochondrial stress induced by antineoplasic agents (doxorubicin and vinblastin) in rats.
To assess the oxidative stress and mitochondrial dysfunction associated with disease, toxic process and aging, in vivo and in vitro preventive effect of propolis extract against mitochondrial oxidative stress induced by two anticancer drugs (doxorubicin and vinblastin) have been investigated in female wistar rat using liver and heart mitochondria. The results show that doxorubicin and vinblastin altered mitochondrial functions as observed by a decrease in respiratory control value, an activation of swelling and overproduction of superoxide anion. Myocardial tissue from doxorubicin treated rats showed a marked increase in malondialdehyde production, a depletion of reduced glutathione contents and an inhibition of catalase and superoxide dismutase activities. Similar results were also observed in liver tissue. Pretreatment of rats with propolis extract (100 mg/kg/day po) (10(-4) M ip) administered 4 days prior to doxorubicin (20 mg/kg) and/or vinblastin (2 mg/kg) injection, substantially reduced the peroxidative damage in myocardium and hepatic tissues and markedly restored the tissues catalase and SOD activities. The results strongly suggest that propolis extract protects heart and liver tissues from oxidative stress by protecting the mitochondria.
Benguedouar
Lamia
L
Laboratoire de Pharmacologie et Phytochimie, Faculté des Sciences, Université de Jijel. 18000 Jijel, Algérie.
Boussenane
Hanane Nadia
HN
Wided
Kebsa
K
Alyane
Mohamed
M
Rouibah
Hassiba
H
Lahouel
Mesbah
M
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D045424:Complex Mixtures / Q000494:pharmacology; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000633:toxicity; D064420:Drug-Related Side Effects and Adverse Reactions / Q000188:drug therapy*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014747:Vinblastine / Q000633:toxicity
D000970:Antineoplastic Agents; D000975:Antioxidants; D045424:Complex Mixtures; D014747:Vinblastine; D004317:Doxorubicin; D011429:Propolis; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase