title: Effects of ginsenoside Rb1 at low doses on histamine, substance P, and monocyte chemoattractant protein 1 in the burn wound areas during the process of acute burn wound repair.
abstract: OBJECTIVE
  We reported recently that the facilitating effects of ginsenosid Rb(1) on burn wound-healing might be due to the promotion of angiogenesis. Increased histamine, substance P (SP), and monocyte chemoattractant protein (MCP)-1 levels caused inflammation, and pain following severe burn wound injury.
  
  
  METHODS
  We examined the effects of ginsenoside Rb1 on the histamine, SP, and MCP-1 levels in burn wound tissue during burn wound repair.
  
  
  CONCLUSIONS
  Ginsenoside Rb1 (1 ng/wound) and basic fibroblast growth factor (bFGF) (2.5 microg/wound) significantly increased the levels of MCP-1 on day 1 compared to the MCP-1 level in vehicle-treated mice. Histamine production of the burn wound area on day 7 was increased by topical application of ginsenoside Rb1 (100 fg-1 ng/wound) and bFGF. The number of mast cells migrating to the burn wound area was also increased by ginsenoside Rb1. Conversely, the increased SP production was reduced by ginsenoside Rb1. This finding suggests that the pain induction by burn injury may be reduced by ginsenoside Rb1. The facilitating actions of ginsenoside Rb1 on burn wound healing may be due to the increase in histamine production via the increase in mast cell migration to the burn wound area induced by the rapid elevation of MCP-1.
authors.affiliation: Division of Functional Histology, Department of Functional Biomedicine, Graduate School of Medicine, Ehime University, Toon City, Ehime 791-0295, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kawahira
authors.forename: Maho
authors.initials: M
authors.lastname: Sumiyoshi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Sakanaka
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism*; D002452:Cell Count; D018932:Chemokine CCL2 / Q000378:metabolism*; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology*; D006632:Histamine / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000473:pathology; D013373:Substance P / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D018932:Chemokine CCL2; D036145:Ginsenosides; D013373:Substance P; C442759:ginsenoside Rb1; D006632:Histamine