Modulatory effect of Gynandropsis gynandra L. on glucose metabolizing enzymes in aflatoxin B1-induced hepatocellular carcinoma in rats.
The modulation of glucose-metabolizing enzymes activities play a vital role in the depletion of energy metabolism and leads to inhibition of cancer growth. In the present study, the effect of Gynandropsis gynandra L. extract on aflatoxin B1 (AFB1)-induced hepatocellular carcinoma (HCC) was studied on glucose-metabolizing enzymes in rats. A significant increase (p < 0.001) in the activities of the key glycolytic enzymes viz., hexokinase and phosphoglucoisomerase, with a significant decrease (p < 0.001) in the gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatase were observed in HCC-bearing rats, when compared with the control. Administration of G. gynandra extract caused a significant decrease in the activities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values. Thus, findings suggest the G. gynandra extract has a definite modulating role on the key enzymes of glucose metabolism in HCC. The modulatory effect may be due to the phytoactive constituents present in the extract of G. gynandra.
Sivanesan
D
D
Unit of Biochemistry, Department of Siddha Medicine, Faculty of Science, Tamil University, Thanjavur-613 005, Tamil Nadu, India.
Begum
V Hazeena
VH
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000201:enzymology*; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced / Q000201:enzymology; D008297:Male; D010936:Plant Extracts / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts; D016604:Aflatoxin B1; D006593:Hexokinase; D006597:Fructose-Bisphosphatase; D005952:Glucose-6-Phosphatase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose