title: New approaches to tuberculosis surveillance in nonhuman primates.
abstract: Despite significant progress in reducing the incidence of tuberculosis in nonhuman primates (NHPs) maintained in captivity, outbreaks continue to occur in established colonies, with potential serious consequences in human exposures, animal losses, disruption of research, and costs related to disease control efforts. The intradermal tuberculin skin test (TST) using mammalian old tuberculin (MOT) has been the mainstay of NHP tuberculosis surveillance and antemortem diagnosis for more than 60 years. But limitations of the TST, particularly its inability to reliably identify animals with latent TB infections, make it unsuitable for use as a single, standalone test for TB surveillance in nonhuman primates in the 21st century. Advances in technology and the availability of Mycobacterium spp. genomic sequence data have facilitated the development and evaluation of new immune-based screening assays as possible adjuncts and alternatives to the TST, including in vitro whole blood assays that measure the release of interferon gamma in response to stimulation with tuberculin or specific mycobacterial antigens, and assays that detect antibodies to highly immunogenic secreted proteins unique to M. tuberculosis, M. bovis, and other species belonging to the M. tuberculosis complex. It is becoming apparent that no single screening test will meet all the requirements for surveillance and diagnosis of tuberculosis in nonhuman primates. Instead, the use of several tests in combination can increase the overall sensitivity and specificity of screening and surveillance programs and likely represents the future of TB testing in nonhuman primates. In this article we describe the characteristics of these newer screening tests and discuss their potential contributions to NHP tuberculosis surveillance programs.
authors.affiliation: Pathogen Detection Laboratory, California National Primate Research Center, University of California, Davis, CA 95616, USA. nwlerche@ucdavis.edu
authors.forename: Nicholas W
authors.initials: NW
authors.lastname: Lerche
authors.forename: JoAnn L
authors.initials: JL
authors.lastname: Yee
authors.forename: Saverio V
authors.initials: SV
authors.lastname: Capuano
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Flynn
mesh_terms: D000818:Animals; D008403:Mass Screening / Q000379:methods; D011323:Primates; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000517:prevention & control*