Effects of bFGF on suppression of collagen type I accumulation and scar tissue formation during wound healing after mucoperiosteal denudation of rat palate.
OBJECTIVE
The purpose of this study was to evaluate the effect of basic fibroblast growth factor (bFGF) on collagen changes after mucoperiosteal denudation of rat palate.


METHODS
A total of 36 male Wistar rats were divided into control, scar, sham, and bFGF groups. In the scar, sham, and bFGF groups, lateral palatal mucoperiosteum was excised to form scar tissue on the palate. In the bFGF group, bFGF solution was injected into the operated area 1 week postoperatively. At 6 weeks postoperatively, the distribution of collagen type I and the 3-dimensional structure of collagen fibers were investigated under immunofluorescent and scanning electron microscopy.


RESULTS
In the bFGF group, weakly immunostained submucosa was clearly distinguishable from the strongly immunostained cervical periodontal ligament and gingiva. Collagen fibers running from submucosal tissue into the surface of underlying palatal bone comprised loosely arranged collagen fibrils. Lumen structures in collagen fibers resembled those in the control group.


CONCLUSIONS
Administration of bFGF for suppression of collagen type I generation could suppress scar tissue formation and reduce connective strength with adjacent teeth and palatal bone.
Choi
Wookjin
W
Section of Orthodontics, Department of Oral Growth & Development, Division of Clinical Dentistry, Fukuoka Dental College, Japan. chwjchwj@hanmail.net
Kawanabe
Hitoshi
H
Sawa
Yoshihiko
Y
Taniguchi
Kunihisa
K
Ishikawa
Hiroyuki
H
D000818:Animals; D002921:Cicatrix / Q000517:prevention & control*; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009061:Mouth Mucosa / Q000601:surgery; D021362:Palate, Hard / Q000601:surgery*; D010521:Periosteum / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*
D024042:Collagen Type I; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2