title: Correlation of near infrared absorption and diffuse reflectance spectroscopy scattering with tissue neovascularization and collagen concentration in a diabetic rat wound healing model.
abstract: The objective of this paper was to correlate optical changes of tissue during wound healing measured by near infrared (NIR) and diffuse reflectance spectroscopy (DRS) with histologic changes in an animal model. Amplitude and phase of scattered light were obtained in a diabetic rat and control model and biopsies were taken for blood vessel ingrowth and collagen concentration. NIR absorption coefficient correlated with blood vessel ingrowth over time, in both the control and diabetic animals. DRS data correlated with collagen concentration. Previous publications by this group documented only the NIR changes during the wound healing process but this is the first reported correlation with histology data. The ability to correlate DRS scattering with collagen concentration during healing is another important and novel finding. This technology may play an important role clinically in assessing the efficacy of wound healing agents in diabetics.
authors.affiliation: Drexel University College of Medicine, Philadelphia, Pennsylvania 19107, USA. Michael.Weingarten@DrexelMed.edu
authors.forename: Michael S
authors.initials: MS
authors.lastname: Weingarten
authors.forename: Elisabeth S
authors.initials: ES
authors.lastname: Papazoglou
authors.forename: Leonid
authors.initials: L
authors.lastname: Zubkov
authors.forename: Linda
authors.initials: L
authors.lastname: Zhu
authors.forename: Michael
authors.initials: M
authors.lastname: Neidrauer
authors.forename: Guy
authors.initials: G
authors.lastname: Savir
authors.forename: Kim
authors.initials: K
authors.lastname: Peace
authors.forename: John G
authors.initials: JG
authors.lastname: Newby
authors.forename: Kambiz
authors.initials: K
authors.lastname: Pourrezaei
mesh_terms: D000818:Animals; D002452:Cell Count; D003094:Collagen / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D006652:Histological Techniques; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D054772:Rats, Hairless; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation; D012867:Skin / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D013053:Spectrophotometry; D019265:Spectroscopy, Near-Infrared; D014945:Wound Healing / Q000502:physiology
chemical_list: D003094:Collagen