title: Sequencing of DNA by free-solution capillary electrophoresis using a genetically engineered protein polymer drag-tag.
abstract: We demonstrate the first use of a non-natural, genetically engineered protein polymer drag-tag to sequence DNA fragments by end-labeled free-solution electrophoresis (ELFSE). Fluorescently labeled DNA fragments resulting from the Sanger cycle sequencing reaction were separated by free-solution capillary electrophoresis, with much higher resolution and cleaner results than previously reported for this technique. With ELFSE, size-based separation of DNA in the absence of a sieving matrix is enabled by the end-on attachment of a polymeric "drag-tag" that modifies the charge-to-friction ratio of DNA in a size-dependent fashion. Progress in ELFSE separations has previously been limited by the lack of suitable large, monodisperse drag-tags. To address this problem, we designed, constructed, cloned, expressed, and purified a non-natural, genetically engineered 127mer protein polymer for use as an ELFSE drag-tag. The Sanger cycle sequencing reaction is performed with the drag-tag covalently attached to the sequencing primer, a major advance over previous strategies for ELFSE sequencing. The electrophoretic separation is diffusion-limited, without significant adsorption of the drag-tag to capillary walls. Although the read length (at about 180 bases) is still short, our results provide evidence that larger protein polymer drag-tags, currently under development, could extend the read length of ELFSE to more competitive levels. ELFSE offers the possibility of very rapid DNA sequencing separations without any of the difficulties associated with viscous polymeric sieving networks and hence will be amenable to implementation in microchannel and chip-based electrophoresis systems.
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Meagher
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Won
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Coyne
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lin
authors.forename: Annelise E
authors.initials: AE
authors.lastname: Barron
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D005813:Genes, Synthetic; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D015202:Protein Engineering / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012996:Solutions
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D012996:Solutions; D004247:DNA