title: Crystallization and preliminary X-ray studies of ferredoxin-NAD(P)+ reductase from Chlorobium tepidum.
abstract: Ferredoxin-NAD(P)(+) reductase (FNR) is a key enzyme that catalyzes the photoreduction of NAD(P)(+) to generate NAD(P)H during the final step of the photosynthetic electron-transport chain. FNR from the green sulfur bacterium Chlorobium tepidum is a homodimeric enzyme with a molecular weight of 90 kDa; it shares a high level of amino-acid sequence identity to thioredoxin reductase rather than to conventional plant-type FNRs. In order to understand the structural basis of the ferredoxin-dependency of this unique photosynthetic FNR, C. tepidum FNR has been heterologously expressed, purified and crystallized in two forms. Form I crystals belong to space group C222(1) and contain one dimer in the asymmetric unit, while form II crystals belong to space group P4(1)22 or P4(3)22. Diffraction data were collected from a form I crystal to 2.4 A resolution on the synchrotron-radiation beamline NW12 at the Photon Factory.
authors.affiliation: Department of Life Sciences, University of Tokyo, Komaba, Tokyo 153-8902, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Muraki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Seo
authors.forename: Tomoo
authors.initials: T
authors.lastname: Shiba
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sakurai
authors.forename: Genji
authors.initials: G
authors.lastname: Kurisu
mesh_terms: D041883:Chlorobium / Q000201:enzymology* / Q000235:genetics; D003460:Crystallization; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D005287:Ferredoxin-NADP Reductase