Restraint increases afebrile body temperature but attenuates fever in Pekin ducks (Anas platyrhynchos).
In mammals, procedures such as handling, restraint, or exposure to open spaces induces an increase in body temperature (T(b)). The increase in temperature shares some characteristics with pyrogen-induced fever and so is often called "stress fever." Birds also respond to acute handling with a stress fever, which may confound thermoregulatory studies that involve animal restraint. We have measured the T(b) responses of Pekin ducks on days when they were restrained and compared them to days when the birds remained unrestrained. Restraint induced a 0.5 degrees C increase in T(b) that was sustained for the entire 8 h of restraint. To determine whether the restraint-induced increase in T(b) is mediated by prostaglandins (PGs) we compared the T(b) responses during restraint after intraperitoneal injection with saline to the responses during restraint after injection with diclofenac sodium (15 mg/kg). There was no difference in response, suggesting that restraint affects T(b) by a PG-independent mechanism. We also compared the T(b) response to intramuscular injection of lipopolysaccharide (LPS; 100 microg/kg), a bacterial pyrogen, when the ducks were restrained or unrestrained. Despite T(b) being higher at the time of LPS injection when the ducks were restrained, the maximum temperature reached after LPS injection was higher, and the period that T(b) remained elevated was longer when the ducks were unrestrained. We conclude that restraint should be considered as a potential confounder in thermoregulatory studies in birds and presumably other species too.
Gray
David A
DA
School of Physiology, University of the Witwatersrand Medical School, Parktown, Johannesburg, South Africa. david.gray@wits.ac.za
Maloney
Shane K
SK
Kamerman
Peter R
PR
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology*; D001833:Body Temperature Regulation / Q000187:drug effects / Q000502:physiology; D004008:Diclofenac / Q000494:pharmacology; D004372:Ducks / Q000502:physiology*; D005260:Female; D005334:Fever / Q000175:diagnosis / Q000503:physiopathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D011453:Prostaglandins / Q000502:physiology; D011749:Pyrogens / Q000494:pharmacology; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000503:physiopathology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D011453:Prostaglandins; D011749:Pyrogens; D004008:Diclofenac