title: [Molecular characterization of cotton leaf Curl Multan virus and its satellite DNA that infects Hibiscus rosa-sinensis].
abstract: Virus isolate G6 was obtained from Hibiscus rosa-sinensis showing yellow and leaf curl symptoms in Guangzhou, Guangdong Province. The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides encoding six potential ORFs. Comparison showed that G6 DNA-A had more than 89% sequence identify with all isolates of Cotton leaf curl Multan virus (CLCuMV) and shared the highest sequence identify (96.1%) with CLCuMV isolate 62. G6 DNA-A had 87.1%-89.8% sequence identity with those of CLCuRV isolates, while less than 87% identities with other begomoviruses. Phylogenetic analysis of G6 DNA-A and selected begomoviruses showed that G6 was most closely related to CLCuMV isolates, and they clustered together as a separate branch. Satellite DNA molecule (G6 DNAbeta) was found to be associated with G6 using the primers beta01 and beta02. G6 DNAbeta contains 1346 nucleotides, with a potential functional ORF (C1) in complementary sense DNA. Pairwise comparison indicated that G6 DNAbeta had the highest sequence identities with CLCuMV DNAbeta (92.1%) and CLCuRV DNAbeta (88.7%), but less than 80% sequence identities with other reported satellite DNA molecules. Phylogenetic analysis indicated that G6 DNAbeta was most closely related to CLCuMV DNAbeta and the two DNAbetas clustered together as a separate branch, and formed the main branch with DNAbeta of CLCuRV and MYVV-Y47. It is concluded that G6 infecting Hibiscus rosa-sinensis is an isolate of CLCuMV.
authors.affiliation: Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
authors.forename: Ming-Jie
authors.initials: MJ
authors.lastname: Mao
authors.forename: Zi-Fu
authors.initials: ZF
authors.lastname: He
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D004276:DNA, Satellite / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D017791:Geminiviridae / Q000145:classification / Q000235:genetics*; D003368:Gossypium / Q000821:virology*; D031584:Hibiscus / Q000821:virology*; D010802:Phylogeny
chemical_list: D004276:DNA, Satellite; D004279:DNA, Viral