Collagen-based biomaterials and cartilage engineering. Application to osteochondral defects.
Articular cartilage has a limited capacity for self-repair after trauma. Besides the conventional surgical techniques for repairing such defects, treatments involve implantation of autologous cells in suspension or within a variety of cell carrying scaffolds such as hyaluronic acid, alginate, agarose/alginate, fibrin or collagen. For the repair of full-thickness osteochondral defects, tissue engineers started to design single- or bi-phased scaffold constructs often containing hydroxyapatite-collagen composites, usually used as a bone substitute. The purpose of this study was to compare the behavior of bovine chondrocytes cultured in collagen-based scaffolds containing or not hydroxyapatite and cross-linked following two different methods. Calf chondrocytes seeded within Hemotèse and Collapat II sponges (SYMATESE biomaterials), chemically cross-linked with glutaraldehyde or EDC/NHS, were maintained up to one month in culture. The cells exhibited a similar behavior in the four scaffolds regarding proliferation level, deposition of glycosaminoglycans in the scaffolds and gene expression of types I, II and X collagens, aggrecan, MMP-1, -13 and the integrin subunits alpha10 and alpha11.
Chajra
H
H
IBCP, Institut de Biologie et Chimie des Protéines, Lyon, France.
Rousseau
C F
CF
Cortial
D
D
Ronzière
M C
MC
Herbage
D
D
Mallein-Gerin
F
F
Freyria
A M
AM
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development*; D019902:Chondrocytes / Q000637:transplantation*; D020219:Chondrogenesis / Q000502:physiology; D003094:Collagen / Q000737:chemistry*; D050724:Fractures, Cartilage / Q000473:pathology* / Q000601:surgery*; D006801:Humans; D023822:Tissue Engineering / Q000639:trends*
D001672:Biocompatible Materials; D003094:Collagen