title: The yeast two-hybrid system and its role in drug discovery.
abstract: BACKGROUND
  The yeast two-hybrid system is the most widely used genetic assay to identify and characterize novel protein interactions. Over the past decade, the system has been adapted to cover an increasingly wide range of applications, including various tasks within the drug discovery and development process.
  
  
  OBJECTIVE
  We highlight the role of different two-hybrid systems within the drug discovery process, including target identification and validation and the selection of affinity reagents for protein targets, such as peptides and small molecules.
  
  
  METHODS
  We have focused on applications where the two-hybrid system has been used to great advantage and have sought to put a special emphasis on less conventional but promising approaches, such as the identification of agents which block therapeutically relevant protein interactions.
  
  
  CONCLUSIONS
  The yeast two-hybrid system has evolved from a method mainly used in basic research to a collection of versatile screening systems with the potential to affect many different aspects of drug discovery today.
authors.affiliation: Dualsystems Biotech AG, Grabenstrasse 11a, 8952 Schlieren, Switzerland.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lentze
authors.forename: Daniel
authors.initials: D
authors.lastname: Auerbach
mesh_terms: D000345:Affinity Labels; D000818:Animals; D016503:Drug Delivery Systems / Q000379:methods*; D015195:Drug Design; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D000345:Affinity Labels; D011506:Proteins