title: Construction of the physical map of the gpa7 locus reveals that a large segment was deleted during rice domestication.
abstract: To facilitate cloning gene(s) underlying gpa7, a deep-coverage BAC library was constructed for an isolate of common wild rice (Oryza rufipogon Griff.) collected from Dongxiang, Jiangxi Province, China (DXCWR). gpa7, a quantitative trait locus corresponding to grain number per panicle, is positioned in the short arm of chromosome 7. The BAC library containing 96,768 clones represents approximate 18 haploid genome equivalents. The contig spanning DXCWR gpa7 was constructed with a series of ordered markers. The putative physical map near the gpa7 locus of another accession of O. rufipogon (Accession: IRGC 105491) was also isolated in silico. Analysis of the physical maps of gpa7 indicated that a segment of about 150 kb was deleted during domestication of common wild rice.
authors.affiliation: Department of Plant Genetics and Breeding and State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, 100094 Beijing, China.
authors.forename: Xianran
authors.initials: X
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Tian
authors.forename: Haiyan
authors.initials: H
authors.lastname: Huang
authors.forename: Lubin
authors.initials: L
authors.lastname: Tan
authors.forename: Zuofeng
authors.initials: Z
authors.lastname: Zhu
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Sun
mesh_terms: D002872:Chromosome Deletion; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics; D020451:Contig Mapping; D015723:Gene Library; D005819:Genetic Markers; D018745:Genome, Plant; D012275:Oryza / Q000235:genetics*; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers