title: A novel non-lens betagamma-crystallin and trefoil factor complex from amphibian skin and its functional implications.
abstract: BACKGROUND
  In vertebrates, non-lens betagamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive.
  
  
  RESULTS
  A naturally existing 72-kDa complex of non-lens betagamma-crystallin (alpha-subunit) and trefoil factor (beta-subunit), named betagamma-CAT, was identified from frog Bombina maxima skin secretions. Its alpha-subunit and beta-subunit (containing three trefoil factor domains), with a non-covalently linked form of alphabeta(2), show significant sequence homology to ep37 proteins, a group of non-lens betagamma-crystallins identified in newt Cynops pyrrhogaster and mammalian trefoil factors, respectively. betagamma-CAT showed potent hemolytic activity on mammalian erythrocytes. The specific antiserum against each subunit was able to neutralize its hemolytic activity, indicating that the two subunits are functionally associated. betagamma-CAT formed membrane pores with a functional diameter about 2.0 nm, leading to K(+) efflux and colloid-osmotic hemolysis. High molecular weight SDS-stable oligomers (>240-kDa) were detected by antibodies against the alpha-subunit with Western blotting. Furthermore, betagamma-CAT showed multiple cellular effects on human umbilical vein endothelial cells. Low dosages of betagamma-CAT (25-50 pM) were able to stimulate cell migration and wound healing. At high concentrations, it induced cell detachment (EC(50) 10 nM) and apoptosis. betagamma-CAT was rapidly endocytosed via intracellular vacuole formation. Under confocal microscope, some of the vacuoles were translocated to nucleus and partially fused with nuclear membrane. Bafilomycin A1 (a specific inhibitor of the vacuolar-type ATPase) and nocodazole (an agent of microtuble depolymerizing), while inhibited betagamma-CAT induced vacuole formation, significantly inhibited betagamma-CAT induced cell detachment, suggesting that betagamma-CAT endocytosis is important for its activities.
  
  
  CONCLUSIONS
  These findings illustrate novel cellular functions of non-lens betagamma-cyrstallins and action mechanism via association with trefoil factors, serving as clues for investigating the possible occurrence of similar molecules and action mechanisms in mammals.
authors.affiliation: Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Shu-Bai
authors.initials: SB
authors.lastname: Liu
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: He
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Lee
authors.forename: Jin-Qiao
authors.initials: JQ
authors.lastname: Qian
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
authors.forename: Yang
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000663:Amphibians; D000818:Animals; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003459:Crystallins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D012867:Skin / Q000378:metabolism*; D000071164:Trefoil Factor-2
chemical_list: D003459:Crystallins; D010455:Peptides; D000071164:Trefoil Factor-2