Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds.
Two abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when seeds were artificially dried. FsClo1 codes for a caleosin, a calcium-binding protein. By expressing FsCLO1 as a fusion protein in Escherichia coli, we obtained biochemical evidence of its ability to bind calcium, a second messenger involved in ABA signaling. This cDNA clone is seed specific, shows a LEA expression pattern and is also induced by ABA and dehydration treatments. The relationship of both genes with seed responses to ABA is discussed.
Jiménez
Jesús Angel
JA
Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias, Facultad de Biología, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain.
Alonso-Ramírez
Ana
A
Nicolás
Carlos
C
D000040:Abscisic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002999:Clone Cells; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D055864:Droughts; D047108:Embryonic Development / Q000187:drug effects; D029964:Fagus / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects*; D014867:Water / Q000378:metabolism
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; C066246:late embryogenesis abundant protein, plant; D014867:Water; D000040:Abscisic Acid; D002118:Calcium