Evaluation of expanded bed adsorption chromatography for extraction of prothrombin complex from Cohn Supernatant I.
This study evaluated the feasibility of substituting expanded bed adsorption (EBA) chromatography for an existing chromatographic purification process for the isolation of prothrombin complex concentrate (PCC) from Cohn Supernatant I. The EBA chromatography (Streamline) resins were compared to the current DEAE-cellulose resin for the extraction of PCC from Cohn SNI. EBA chromatography resins efficiently bound PCC from Cohn SNI at a significantly higher flow rate of up to 300 cm/h compared to 30 cm/h for the current DEAE-cellulose process. Composition and yield of the recovered PCC reflected the elution conditions used. The results indicate that EBA chromatography could be used to efficiently produce PCC comparable to existing products.
McCann
Karl B
KB
Research and Development Department, CSL Bioplasma, 189-209 Camp Road, Broadmeadows, Victoria 3047, Australia. karl.mccann@csl.com.au
Gomme
Peter T
PT
Wu
John
J
Bertolini
Joseph
J
D000837:Anion Exchange Resins / Q000494:pharmacology; D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D001798:Blood Proteins / Q000737:chemistry*; D002845:Chromatography / Q000379:methods*; D019411:Clinical Laboratory Techniques; D004345:Drug Industry / Q000379:methods; D006801:Humans; D011516:Prothrombin / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000837:Anion Exchange Resins; D001779:Blood Coagulation Factors; D001798:Blood Proteins; C047487:Cohn fraction I; D012710:Serum Albumin, Bovine; C025667:prothrombin complex concentrates; D011516:Prothrombin