[Polymorphisms of human cytomegalovirus UL139 open reading frame in Hirschsprung's disease].
OBJECTIVE
To explore the UL139 gene polymorphism of human cytomegalovirus (HCMV), and the relationship between polymorphisms of HCMV UL139 ORF and Hirschsprung's disease (HD).


METHODS
Forty-three specimens of the aganglionic intestinal segment and 6 urine samples of HD infants were amplified by nested polymerase chain reaction (PCR) method. The amplicons were sequenced in both strands directly. The control group consisted of 10 asymptomatic HCMV infected infants, and their urine specimens were also analyzed using the same method.


RESULTS
HCMV UL139 genes of 28 clinical strains from HD 1 patients were successfully amplified and sequenced. UL139 was hypervariable and was clustered into 3 major groups and 5 genotypes. The predominant genotype of HCMV in HD infants was UL139 Group 3 (48 percent). Comparison of strains distribution between the two groups did not reach statistical significance using chi square test (chi square=7.378, P=0.194). The results of correlation analysis between UL139 and UL144 genes showed a p value 0.05 by Kendall test. Clinically, strains from the rectosigmoid segment, total colon aganglionosis, and long-segment were distributed sporadically in UL139 genotypes.


CONCLUSIONS
UL139 gene displayed polymorphisms. No linkage was found between UL139 genotype and clinical phenotype of HD. There was no correlation between HCMV UL144 and UL139.
Mao
Zhi-quin
ZQ
Department of Pediatrics, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, China.
Huang
Ying
Y
Ruan
Qiang
Q
Sun
Mei
M
Qi
Ying
Y
Gao
Hong
H
D003587:Cytomegalovirus / Q000235:genetics*; D005260:Female; D005838:Genotype; D006627:Hirschsprung Disease / Q000209:etiology / Q000821:virology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D008562:Membrane Glycoproteins; C119624:UL144 ORF protein, Human herpesvirus 5; D014764:Viral Proteins