Determination of chloramphenicol in shrimp by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS).
A liquid chromatographic method with electrospray ionization tandem mass spectrometric (LC-ESI-MS-MS) detection and identification is described for the determination of chloramphenicol (CAP) in shrimp tissue. Homogenized shrimp samples were extracted by liquid-liquid extraction using ethyl acetate. d5-Chloramphenicol (5D-CAP) was used as the internal standard. Data acquisition was in negative-ion multiple reaction monitoring (NMRM) mode using three transition reactions for CAP (m/z 321 --> 152, m/z 321 --> 257 and m/z 321 --> 194) and two for d5-chloramphenicol (m/z 326 --> 262 and m/z 326 --> 157). Method validation was carried out according to European Commission decision 2002/657/EC. The calibration curve was linear in the range 0.10-2.00 microg l(-1), with typical r2 > 0.99. The decision limit (CC alpha) and detection capability (CC beta) were 0.06 and 0.10 microg kg(-1), respectively. There was no influence of the matrix on the determination of chloramphenicol.
Tyagi
A
A
Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575 002, India.
Vernekar
P
P
Karunasagar
Iddya
I
Karunasagar
Indrani
I
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D005060:Europe; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D033561:Penaeidae / Q000737:chemistry*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000032:analysis*
D000900:Anti-Bacterial Agents; D002701:Chloramphenicol