Evaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis protocol for rapid subtyping of Vibrio parahaemolyticus.
We evaluated the cost-effectiveness of the restriction enzymes with rare-cutting sites in the genome of Vibrio parahaemolyticus RIMD 2210633 for pulsed-field gel electrophoresis (PFGE) analysis. The evaluation indicated that PFGE with both NotI and SfiI was discriminatory, but NotI was more cost-effective. Based on the results of this study, we suggest using NotI and SfiI as the 1st and the 2nd restriction enzyme for standardizing the PulseNet PFGE protocol for molecular subtyping and global surveillance of V. parahaemolyticus.
Wang
You-Wun
YW
The Central Region Laboratory, Center of Research and Diagnostics, Centers for Disease Control, Taichung City 40855, Taiwan.
Chern
Lih-Ling
LL
Cam
Phung Dac
PD
Chiou
Chien-Shun
CS
D015373:Bacterial Typing Techniques / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000191:economics; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000191:economics / Q000592:standards*; D005838:Genotype; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes