Investigation of killer cell immunoglobulin-like receptor gene diversity in KIR3DL1 and KIR3DS1 in a transplant population.
Several overlapping amplicons were used to obtain the sequence of genomic DNA covering most of the coding regions of KIR3DL1 and KIR3DS1 from a family and 77 bone marrow transplant patients and their unrelated donors. Alleles 3DL1*00101 and *002 were most frequently observed in addition to 12 other known 3DL1 alleles. A single 3DS1 allele, 3DS1*01301, was identified in the 31 of 32 individuals carrying this gene. Two new alleles, 3DL1*01702 and 3DS1*058, were characterized. Three samples appeared to carry the duplicated killer cell immunoglobulin-like receptor (KIR) haplotype observed in other studies based on the presence of 3DS1 and two 3DL1 alleles. Additionally, one sample appeared to carry a novel KIR haplotype containing one 3DL1 and two 3DS1 alleles.
Belle
I
I
Department of Pediatrics, CW Bill Young Marrow Donor Recruitment and Research Program, Georgetown University Medical Center, Washington, DC 20057, USA.
Hou
L
L
Chen
M
M
Steiner
N K
NK
Ng
J
J
Hurley
C K
CK
D000483:Alleles; D001402:B-Lymphocytes; D016026:Bone Marrow Transplantation; D002460:Cell Line; D006239:Haplotypes; D006801:Humans; D007694:Killer Cells, Natural; D054341:Receptors, KIR3DL1 / Q000235:genetics*; D054349:Receptors, KIR3DS1 / Q000235:genetics*
D054341:Receptors, KIR3DL1; D054349:Receptors, KIR3DS1