title: Complete nucleotide sequence and organization of the mitogenome of the silk moth Caligula boisduvalii (Lepidoptera: Saturniidae) and comparison with other lepidopteran insects.
abstract: The 15,360-bp long complete mitogenome of Caligula boisduvalii possesses a gene arrangement and content identical to other completely sequenced lepidopteran mitogenomes, but different from the common arrangement found in most insect order, as the result of the movement of tRNA(Met) to a position 5'-upstream of tRNA Ile. The 330-bp A+T-rich region is apparently capable of forming a stem-and-loop structure, which harbors the conserved flanking sequences at both ends. Dissimilar to what has been seen in other sequenced lepidopteran insects, the initiation codon for C. boisduvalii COI appears to be TTG, which is a rare, but apparently possible initiation codon. The ATP8, ATP6, ND4L, and ND6 genes, which neighbor another PCG at their 3' end, all harbored potential sequences for the formation of a hairpin structure. This is suggestive of the importance of such structures for the precise cleavage of the mRNA of mature PCGs. Phylogenetic analyses of available sequenced species of Bombycoidea, Pyraloidea, and Tortricidea supported the morphology-based current hypothesis that Bombycoidea and Pyraloidea are monophyletic (Obtectomera). As previously suggested, Bombycidae (Bombyx mori and B. mandarina) and Saturniidae (Antheraea pernyi and C. boisduvalii) formed a reciprocal monophyletic group.
authors.affiliation: College of Agriculture & Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Mee Yeon
authors.initials: MY
authors.lastname: Hong
authors.forename: Eun Mee
authors.initials: EM
authors.lastname: Lee
authors.forename: Yong Hun
authors.initials: YH
authors.lastname: Jo
authors.forename: Hae Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Seong Ryul
authors.initials: SR
authors.lastname: Kim
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.forename: Byung Rae
authors.initials: BR
authors.lastname: Jin
authors.forename: Pil Don
authors.initials: PD
authors.lastname: Kang
authors.forename: Ki-Gyoung
authors.initials: KG
authors.lastname: Kim
authors.forename: Yeon Soo
authors.initials: YS
authors.lastname: Han
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
mesh_terms: D020856:AT Rich Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D017344:Genes, Insect; D049750:Genome, Insect; D054629:Genome, Mitochondrial; D019476:Insect Proteins / Q000235:genetics; D009036:Moths / Q000145:classification / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D003062:Codon; D004272:DNA, Mitochondrial; D019476:Insect Proteins