title: Stabilization of scleral collagen by glycerol aldehyde cross-linking.
abstract: The paper aims at the evaluation of prospects for using glyceraldehyde as a cross-linking agent for the scleral tissue. Stability parameters (denaturation temperature, Young's modulus, ultimate tensile stress, proteolytic resistance) and analytical parameter (fluorescence intensity) were determined during the glycation process of isolated rabbit sclera. The analysis of fluorescence spectral characteristic provided information about some glycation products. The glyceraldehyde treatment was resulted in a significant increase in thermal stability, proteolytic resistance and improvement of biomechanical characteristics (Young's modulus, ultimate tensile stress). Unique properties of the reaction between scleral collagen and glyceraldehyde are observed at short cross-linking times. The appearance of intermediate collagen fraction with lowest thermal and proteolytic stability was detected.
authors.affiliation: Moscow State University, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Danilov
authors.forename: N Yu
authors.initials: NY
authors.lastname: Ignatieva
authors.forename: E N
authors.initials: EN
authors.lastname: Iomdina
authors.forename: S A
authors.initials: SA
authors.lastname: Semenova
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaya
authors.forename: T E
authors.initials: TE
authors.lastname: Grokhovskaya
authors.forename: V V
authors.initials: VV
authors.lastname: Lunin
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D003094:Collagen / Q000737:chemistry*; D003116:Color; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004548:Elasticity; D010450:Endopeptidases / Q000737:chemistry; D005985:Glyceraldehyde / Q000737:chemistry*; D006031:Glycosylation; D009097:Multienzyme Complexes / Q000737:chemistry; D010206:Papain / Q000737:chemistry; D011489:Protein Denaturation; D011817:Rabbits; D012590:Sclera / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013718:Tensile Strength; D044366:Transition Temperature
chemical_list: D003432:Cross-Linking Reagents; D009097:Multienzyme Complexes; D005985:Glyceraldehyde; D003094:Collagen; D010450:Endopeptidases; D010206:Papain; C089174:morikrase