title: Collagen-based biomaterials and cartilage engineering. Application to osteochondral defects.
abstract: Articular cartilage has a limited capacity for self-repair after trauma. Besides the conventional surgical techniques for repairing such defects, treatments involve implantation of autologous cells in suspension or within a variety of cell carrying scaffolds such as hyaluronic acid, alginate, agarose/alginate, fibrin or collagen. For the repair of full-thickness osteochondral defects, tissue engineers started to design single- or bi-phased scaffold constructs often containing hydroxyapatite-collagen composites, usually used as a bone substitute. The purpose of this study was to compare the behavior of bovine chondrocytes cultured in collagen-based scaffolds containing or not hydroxyapatite and cross-linked following two different methods. Calf chondrocytes seeded within Hemotèse and Collapat II sponges (SYMATESE biomaterials), chemically cross-linked with glutaraldehyde or EDC/NHS, were maintained up to one month in culture. The cells exhibited a similar behavior in the four scaffolds regarding proliferation level, deposition of glycosaminoglycans in the scaffolds and gene expression of types I, II and X collagens, aggrecan, MMP-1, -13 and the integrin subunits alpha10 and alpha11.
authors.affiliation: IBCP, Institut de Biologie et Chimie des Protéines, Lyon, France.
authors.forename: H
authors.initials: H
authors.lastname: Chajra
authors.forename: C F
authors.initials: CF
authors.lastname: Rousseau
authors.forename: D
authors.initials: D
authors.lastname: Cortial
authors.forename: M C
authors.initials: MC
authors.lastname: Ronzière
authors.forename: D
authors.initials: D
authors.lastname: Herbage
authors.forename: F
authors.initials: F
authors.lastname: Mallein-Gerin
authors.forename: A M
authors.initials: AM
authors.lastname: Freyria
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development*; D019902:Chondrocytes / Q000637:transplantation*; D020219:Chondrogenesis / Q000502:physiology; D003094:Collagen / Q000737:chemistry*; D050724:Fractures, Cartilage / Q000473:pathology* / Q000601:surgery*; D006801:Humans; D023822:Tissue Engineering / Q000639:trends*
chemical_list: D001672:Biocompatible Materials; D003094:Collagen