[Study on total alkaloids content and its influential factors in medicinal materials of Tripterygium].
OBJECTIVE
To investigate the total alkaloids content profile and diversity of medicinal materials of Tripterygium.


METHODS
The total alkaloids were extracted by organic solvent and purified by column chromatography (aluminium oxide), and determined by UV spectrometry.


CONCLUSIONS
The total alkaloids content in phloem was 2 to 5 times richer than that in xylem, and their changes had shown concordance. For the different species/types, the content from high to low in order was T. hypoglaucum, Tripterygium F, T. wilfordii and T. regellii, but in each type, the disparity among different populations content was very small. The populations with higher content were in Suichuan, Jiangxi Province, Huangshan, Anhui Province, Jingning, Zhejiang Province and Kunming, Yuannan Province; which came from T. hypoglaucum and Tripterygium F. The degree of difference of total alkaloids content among individuals in each population was different. The populations, which had a rich content diversity, were in Jingning, Zhejiang Province, Taining, Fujian Province and Kunming, Yunnan Province. They will be used for good germplasm selecting. The thickness of medicinal materials did not affect the total alkaloids content in the same plant. The total alkaloids content in xylem changed with seasons: the highest in March, then declining from June to December, the lowest in December, While this did not happen in phloem.
Huang
Wen-Hua
WH
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
Zhang
Rui
R
Guo
Bao-Lin
BL
Si
Jin-Ping
JP
D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry*; D002681:China; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D012621:Seasons; D029757:Tripterygium / Q000737:chemistry*
D000470:Alkaloids; D004365:Drugs, Chinese Herbal