Common marmoset embryonic stem cell can differentiate into cardiomyocytes.
Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.
Chen
Hao
H
Department of Regenerative Medicine and Advanced Cardiac Therapeutics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Hattori
Fumiyuki
F
Murata
Mitsushige
M
Li
Weizhen
W
Yuasa
Shinsuke
S
Onizuka
Takeshi
T
Shimoji
Kenichiro
K
Ohno
Yohei
Y
Sasaki
Erika
E
Kimura
Kensuke
K
Hakuno
Daihiko
D
Sano
Motoaki
M
Makino
Shinji
S
Ogawa
Satoshi
S
Fukuda
Keiichi
K
D000200:Action Potentials; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002144:Callithrix; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D009200:Myocardial Contraction; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D011506:Proteins / Q000032:analysis / Q000378:metabolism
D011506:Proteins; D001973:Bromodeoxyuridine