An induced mass spawn of the hermaphroditic lion-paw scallop, Nodipecten subnodosus: genetic assignment of maternal and paternal parentage.
The Pacific lion-paw scallop is commonly propagated for aquaculture by induced mass spawns of few individuals. Parentage of a mass spawn of this species has not been evaluated nor has the maternal and paternal contribution of each of these functional hermaphrodites to the progeny. Genotypes of 6 spawners and 374 resulting progeny at 6 microsatellite loci were coupled with mitochondrial DNA sequencing to assign maternal and paternal parentage. After the identification of a high proportion of null alleles (9.7%), microsatellite data revealed that 51.7% of the progenies were full siblings, with a significant, unequal contribution of the 6 spawners to the progeny. Three progenies were the result of self-fertilization. All spawners contributed paternally (though unequally); however, 2 spawners were the maternal parents of all but 7 progenies resulting in a variance effective population size of 3.52. DNA sequencing confirmed 4 microsatellite mutations within 4476 alleles scored, all in the paternal germ line. With minor exception, the loci conformed to Mendelian rules of segregation when null alleles were accounted for, and 2 loci were found to be linked. These results lend insight to the genetic composition of induced mass spawns and provide a basis for the development of more effective spawning techniques.
Petersen
Jessica L
JL
Genomic Variation Laboratory, Department of Animal Science, University of California, One Shields Avenue, Davis, CA 95616, USA. jlpetersen@ucdavis.edu
Ibarra
Ana M
AM
Ramirez
José L
JL
May
Bernie
B
D000818:Animals; D017756:Aquaculture; D002140:California; D012734:Disorders of Sex Development / Q000235:genetics*; D005260:Female; D005306:Fertilization / Q000235:genetics / Q000502:physiology*; D005787:Gene Frequency; D005854:Germ Cells / Q000502:physiology; D040582:Inheritance Patterns / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000502:physiology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D049897:Pectinidae / Q000235:genetics*; D023121:RNA, Messenger, Stored / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D023121:RNA, Messenger, Stored; D012336:RNA, Ribosomal, 16S