Hematoporphyrin-mediated fluorescence reflectance imaging: application to early tumor detection in vivo in small animals.
The in vivo early detection of subcutaneous human tumors implanted in small animals was studied by laser-induced fluorescence reflectance imaging (FRI), with a hematoporphyrin (HP) compound as an exogenous optical contrast agent. Tumor detection was shown to be possible just 3 days after the inoculation of tumor cells, when tumors were neither visible nor palpable. However, this detection capability is limited to a temporal window of approximately 100 h from HP administration and to a low optical contrast of the tumor (<2).
Autiero
Maddalena
M
Dipartimento di Scienze Fisiche, Università di Napoli Federico II, Via Cinthia, 80126, Naples, Italy.
Cozzolino
Rosanna
R
Laccetti
Paolo
P
Marotta
Marcello
M
Quarto
Maria
M
Riccio
Patrizia
P
Roberti
Giuseppe
G
D000818:Animals; D045744:Cell Line, Tumor; D055088:Early Detection of Cancer; D006415:Hematoporphyrins; D006801:Humans; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D017319:Photosensitizing Agents; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000209:etiology; D013050:Spectrometry, Fluorescence
D006415:Hematoporphyrins; D017319:Photosensitizing Agents