[Change of endogenous hormone around sprout tumble of Pinellia ternata under high temperature stress].
OBJECTIVE
To study the change of endogenous hormone (ABA, IAA, JA, GA3, ZR) in the leaves, petioles, tubers of Pinellia ternate around sprout tumble. It also provided some valuable information to prevent sprout tumble and increase production.


METHODS
Tubers of P. ternata were cultured firstly at (23 +/- 1) degree C for certain days, and then they were coerced under (30 +/- 1 ) degree C stress in the same artificial climate boxes. The endogenous hormones in leaves, petioles and tubers during different stages of high temperature stress were determined with Enzyme-linked Immunosorbent Assays (ELISA).


RESULTS
After under high temperature stress, ABA content in leaves, petioles and tubers increased obviously. Similarly, JA content rose all in the leaves, petioles and tubers. But in the same conditions IAA content declined significantly in the leaves and petioles. In the tubers, IAA content also decreased, but not quickly. With the extension of high temperature coercion, the leaves, petioles, tubers, ZR content were gradually falling off. In the leaves of GA3 content rose markedly at the third day, fell down at the sixth day, but remained higher than before treatment. With the extension of the processing time, GA3 content fell off in the petioles and tubers.


CONCLUSIONS
ABA, JA, ZT and GA3 played an important role in controlling sprout tumble of P. ternata.
Xue
Jian-Ping
JP
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China. xuejp2000@yahoo.com.cn
Zhang
Ai-Min
AM
Yang
Jian
J
Chang
Li
L
Huang
Yue-Qin
YQ
D000040:Abscisic Acid / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005875:Gibberellins / Q000378:metabolism; D006358:Hot Temperature; D007210:Indoleacetic Acids / Q000378:metabolism; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D030021:Pinellia / Q000378:metabolism* / Q000502:physiology; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D035281:Plant Tubers / Q000378:metabolism; D010946:Plants, Medicinal / Q000378:metabolism / Q000502:physiology
D003517:Cyclopentanes; D005875:Gibberellins; D007210:Indoleacetic Acids; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid; D000040:Abscisic Acid; D007541:Isopentenyladenosine; C009699:zeatin riboside; C007842:gibberellic acid