Anti-oligosaccharide antibodies as tools for studying sulfated sialoglycoconjugate ligands for siglecs and selectins.
Several sulfated sialoglycoconjugates have recently been shown to serve as ligands for selectins and siglecs. For instance, alpha2-->3 sialylated 6-sulfo-Lewis x was found to serve as a ligand for selectins on skin-homing helper memory T cells, and alpha2-->6 sialylated 6-sulfo-LacNAc to be a preferred ligand for CD22/siglec-2 on human naïve B cells. Monoclonal antibodies specific to sulfated sialoglycoconjugates are effective tools to dissect these ligands on minor subsets of human leukocytes.
Kannagi
Reiji
R
Department of Molecular Pathology, Research Institute, Aichi Cancer Center, 1-1 Kanokoden, Chikusaku, Nagoya 464-8681, Japan. rkannagi@aichi-cc.jp
Ohmori
Katsuyuki
K
Kimura
Naoko
N
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001671:Biochemistry / Q000379:methods*; D006001:Glycoconjugates / Q000276:immunology*; D006801:Humans; D037102:Lectins / Q000276:immunology*; D008024:Ligands; D019158:N-Acetylneuraminic Acid / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology*; D019087:Selectins / Q000276:immunology*; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D013431:Sulfates / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D006001:Glycoconjugates; D037102:Lectins; D008024:Ligands; D009844:Oligosaccharides; D019087:Selectins; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D013431:Sulfates; D019158:N-Acetylneuraminic Acid