title: Fatty acids influence binding of cobalt to serum albumin in patients with fatty liver.
abstract: Human serum albumin binds ligands such as fatty acids and metals in circulation. Oxidative stress can modify albumin and affect ligand binding. This study examines the role of oxidative stress and fatty acids in modulating cobalt binding to albumin in patients with fatty liver. Elevated levels of malondialdehyde and protein carbonyls, indicative of oxidative stress were evident in serum of patients with fatty liver. A significant decrease in albumin-cobalt binding was also observed. Albumin isolated from patient serum also showed an increase in bound fatty acids. In vitro experiments indicated that while oxidant exposure or removal of fatty acids independently decreased cobalt binding to albumin, removal of fatty acids from the protein prior to oxidant exposure did not influence the oxidant effect on albumin-cobalt binding. These results suggest that oxidative stress and fatty acids on albumin can influence albumin-cobalt binding in patients with fatty liver by independent mechanisms.
authors.affiliation: The Wellcome Trust Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College, Ida Scudder Road, Vellore-632004, India.
authors.forename: G Jayakumar
authors.initials: GJ
authors.lastname: Amirtharaj
authors.forename: Sathish Kumar
authors.initials: SK
authors.lastname: Natarajan
authors.forename: Ashis
authors.initials: A
authors.lastname: Mukhopadhya
authors.forename: Uday George
authors.initials: UG
authors.lastname: Zachariah
authors.forename: Sudheer K
authors.initials: SK
authors.lastname: Hegde
authors.forename: George
authors.initials: G
authors.lastname: Kurian
authors.forename: K A
authors.initials: KA
authors.lastname: Balasubramanian
authors.forename: Anup
authors.initials: A
authors.lastname: Ramachandran
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D003035:Cobalt / Q000378:metabolism*; D019327:Copper Sulfate / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D005234:Fatty Liver / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism*; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D012709:Serum Albumin; D019820:Xanthine; D003035:Cobalt; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D019327:Copper Sulfate