title: Simple and efficient method for the estimation of residues of flubendiamide and its metabolite desiodo flubendiamide.
abstract: An analytical method was standardized for the estimation of residues of flubendiamide and its metabolite desiodo flubendiamide in various substrates comprising cabbage, tomato, pigeonpea grain, pigeonpea straw, pigeonpea shell, chilli, and soil. The samples were extracted with acetonitrile, diluted with brine solution, and partitioned into chloroform, dried over anhydrous sodium sulfate, and treated with 500 mg of activated charcoal powder. Final clear extracts were concentrated under vacuum and reconstituted into HPLC grade acetonitrile, and residues were estimated using HPLC equipped with a UV detector at 230 lambda and a C18 column. Acetonitrile/water (60:40 v/v) at 1 mL/min was used as mobile phase. Both flubendiamide and desiodo flubendiamide presented distinct peaks at retention times of 11.07 and 7.99 min, respectively. Consistent recoveries ranging from 85 to 99% for both compounds were observed when samples were spiked at 0.10 and 0.20 mg/kg levels. The limit of quantification of the method was worked out to be 0.01 mg/kg.
authors.affiliation: Pesticide Residue Analysis Laboratory, Department of Entomology, Punjab Agricultural University, Punjab, India.
authors.forename: Raminderjit Singh
authors.initials: RS
authors.lastname: Battu
authors.forename: Baljeet
authors.initials: B
authors.lastname: Singh
authors.forename: Rubaljot
authors.initials: R
authors.lastname: Kooner
authors.forename: Balwinder
authors.initials: B
authors.lastname: Singh
mesh_terms: D001549:Benzamides / Q000032:analysis*; D001937:Brassica / Q000737:chemistry; D036561:Cajanus / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D007306:Insecticides; D018551:Solanum lycopersicum / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis*; D012987:Soil / Q000032:analysis; D013450:Sulfones / Q000032:analysis*; D005466:Fluorocarbons; D010797:Phthalimides
chemical_list: D001549:Benzamides; D007306:Insecticides; D010573:Pesticide Residues; D012987:Soil; D013450:Sulfones; C529886:desiodoflubendiamide; C508762:flubendiamide; D005466:Fluorocarbons; D010797:Phthalimides