Low-frequency electromagnetic field effects on functional groups in human skin keratinocytes cells revealed by IR-SNOM.
Human HaCaT cells, exposed for 24 h to a 1 mT (rms) 50 Hz sinusoidal magnetic field in a temperature-regulated solenoid, suffer detectable changes in their biochemical properties and shapes. By using infrared wavelength-selective scanning near-field optical microscopy, we observed changes in the distribution of the inner chemical functional groups and in the cell morphology with a resolution of 80-100 nm.
Cricenti
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A
Istituto di Struttura della Materia, CNR, via Fosso del Cavaliere 100, 00133 Roma, Italy. antonio.cricenti@ism.cnr.it
Generosi
R
R
Luce
M
M
Perfetti
P
P
Sanghera
J S
JS
Aggarwal
I D
ID
Tolk
N H
NH
Vobornik
D
D
Margaritondo
G
G
Piston
D W
DW
Manni
V
V
Grimaldi
S
S
Lisi
A
A
Rieti
S
S
D002460:Cell Line; D004574:Electromagnetic Fields; D006801:Humans; D007259:Infrared Rays; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D020527:Microscopy, Scanning Probe; D012867:Skin / Q000166:cytology*