[Effects of procaine on human nasopharyngeal carcinoma cell strain CNE-2Z].
OBJECTIVE
To study the effects of procaine on human nasopharyngeal carcinoma cell strain CNE-2Z.


METHODS
CNE-2Z cells-cultured in vitro-were exposed to different concentration procaine and different time. Its growth characteristic were studied by using MTT assay and flow cytometry. The expression of p16 and RASSF1A mRNA were analyzed by RT-PCR.


RESULTS
Procaine could inhibit the proliferation of CNE-2Z cells and the effects were in dose- and time-dependent manners. Procaine also arrested CNE-2Z cells in G1 phase of cell cycle. The expression of RASSF1A mRNA was up-regulated in CNE-2Z cells treated with procaine, but no obvious change was found in the expression of p16 mRNA.


CONCLUSIONS
Procaine could inhibit cell growth and up-regulate the expression of RASSF1A mRNA, which may play an important role in CNE-2Z cells' proliferation inhibition induced by procaine.
Zhou
Hui
H
Department of Otorhinolaryngology-Head and Neck Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524023, China.
Xu
Mingfeng
M
Luo
Guoqing
G
Zhang
Yuefei
Y
D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D011343:Procaine / Q000008:administration & dosage / Q000494:pharmacology*; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
C411762:RASSF1 protein, human; D025521:Tumor Suppressor Proteins; D011343:Procaine