Determination of toxaphene specific congeners in fish liver oil and feedingstuff using gas chromatography coupled to high resolution mass spectrometry.
A new method for the determination of nine toxaphene specific congeners in fish liver oil and feedingstuff has been developed. The samples were extracted using pressurized liquid extraction followed by a purification on silica and florisil columns. Identification and quantification were conducted using GC-(EI)-HRMS, and comparison with MS/MS detection was performed, using electron ionization and negative chemical ionization. Limits of detection were ranged from 0.01 to 0.22 microg kg(-1) (12% moisture) as required for feed samples. The calibration curves showed a good linearity for all congeners (R(2)>0.99). Repeatability was below 9% for all the congeners and recoveries were in-between 73 and 86%. This analytical method was applied to the quantification of thirteen real samples collected within national monitoring plans for further risk assessment.
Veyrand
Bruno
B
Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Ecole Nationale Vétérinaire de Nantes (ENVN) Route de Gachet, BP50707, 44307 Nantes cedex 3, France. laberca@vet-nantes.fr
Venisseau
Anaïs
A
Marchand
Philippe
P
Antignac
Jean-Philippe
JP
Le Bizec
Bruno
B
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D005399:Fishes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008099:Liver / Q000737:chemistry*; D009821:Oils / Q000737:chemistry*; D014112:Toxaphene / Q000494:pharmacology*
D009821:Oils; D014112:Toxaphene