Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells.
OBJECTIVE
To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells.


METHODS
Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths.


RESULTS
Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P<0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P<0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P<0.05) adhesion to GP202 clones with larger MUC1 VNTR domains.


CONCLUSIONS
This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of the MUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa.
Costa
Natália R
NR
Institute of Molecular Pathology and Immunology, University of Porto, Porto 4200-465, Portugal.
Mendes
Nuno
N
Marcos
Nuno T
NT
Reis
Celso A
CA
Caffrey
Thomas
T
Hollingsworth
Michael A
MA
Santos-Silva
Filipe
F
D001422:Bacterial Adhesion / Q000235:genetics*; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000382:microbiology* / Q000473:pathology; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D018396:Mucin-1 / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000473:pathology
D018396:Mucin-1