Simultaneous protection against allograft rejection and graft-versus-host disease after total lymphoid irradiation: role of natural killer T cells.
BACKGROUND
The use of combined organ and bone marrow transplantation has been studied extensively in rodent models to induce immune tolerance to organ grafts. However, bone marrow transplants with mature donor T cells can induce graft-versus-host disease even in human leukocyte antigen-matched humans. We determined whether total lymphoid irradiation can simultaneously protect against graft-versus-host disease while facilitating tolerance.


METHODS
To more closely model clinical studies, we added mature donor T cells to bone marrow grafts combined with heart grafts, and compared murine graft and host survival after conditioning with nonmyeloablative total body or total lymphoid irradiation and depletive anti-T-cell antibodies.


RESULTS
Conditioning with total lymphoid irradiation protected hosts against both graft-versus-host disease and organ graft rejection. Although nonmyeloblative total body irradiation prevented organ graft rejection, all hosts succumbed to lethal graft-versus host disease. Induction of tolerance with total lymphoid irradiation and anti-T-cell antibodies was dependent on the presence of regulatory host natural killer T cells, and expression of CD1d on donor marrow but not heart graft cells.


CONCLUSIONS
Conditioning with total lymphoid irradiation and anti-T-cell antibodies prevented host-versus-donor and donor-versus-host alloimmune responses. Tolerance required host natural killer T-cell recognition of CD1d on donor marrow cells.
Liu
Yin Ping
YP
Division of Immunology & Rheumatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305-5166, USA.
Li
Zengqi
Z
Nador
Roland G
RG
Strober
Samuel
S
D000818:Animals; D018949:Antigens, CD1 / Q000235:genetics; D016026:Bone Marrow Transplantation / Q000276:immunology*; D006084:Graft Rejection / Q000517:prevention & control*; D006085:Graft Survival; D006086:Graft vs Host Disease / Q000517:prevention & control*; D016027:Heart Transplantation / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D014184:Transplantation, Homologous / Q000276:immunology*; D014916:Whole-Body Irradiation
D018949:Antigens, CD1