title: Molecular detection and antimicrobial resistance of diarrheagenic Escherichia coli strains isolated from diarrheal cases.
abstract: OBJECTIVE
  To identify and classify Iranian isolates of diarrheagenic Escherichia coli (E. coli) on the basis of presence of virulence genes and to determine antibiotic susceptibility of isolated strains.
  
  
  METHODS
  The current cross-sectional study was conducted in 2005 at the Pasteur Institute, Tehran, Iran. One hundred and ninety-three diarrheagenic E. coli isolated from diarrheal patients in different regions of Iran were included in current study. Virulence factor genes for diarrheagenic E. coli were detected by polymerase chain reaction.
  
  
  RESULTS
  Of the 193 diarrheagenic E. coli detected by PCR, 86 (44.5%) were Shiga toxin-producing E. coli STEC, 74 (38.4%) enteropathogenic E. coli EPEC, 19 (9.8%) enteroaggregative E. coli, and 14 (7.3%) enterotoxigenic E. coli isolates. Susceptibility to 12 clinically important antimicrobial agents was determined for 193 strains of diarrheagenic E. coli. A high incidence of resistance to tetracycline (63%), ampicillin (62%), streptomycin (56%), amoxicillin/clavulanic acid (44.5%), trimethoprim/sulfamethoxazole (39.5%), and cephalothin (37%) was observed.
  
  
  CONCLUSIONS
  The STEC and EPEC strains with high resistance to tetracycline and ampicillin, but highly susceptible to quinolones are among the most important causative agent of diarrhea in Iran. This study suggests that antimicrobial resistance is widespread among E. coli strains colonizing Iranian patients. Guidelines for appropriate use of antibiotics in developing countries require updating.
authors.affiliation: Enterobacteriaceae Laboratory, Department of Microbiology, No. 69 Pasteur Avenue, Institute Pasteur of Iran, Tehran, Iran. mmaslani@yahoo.com
authors.forename: Mehdi M
authors.initials: MM
authors.lastname: Aslani
authors.forename: Siavosh Salmanzadeh-Ahrabi
authors.initials: SS
authors.lastname: Ahrabi
authors.forename: Yousef M
authors.initials: YM
authors.lastname: Alikhani
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Jafari
authors.forename: Reza M
authors.initials: RM
authors.lastname: Zali
authors.forename: Monireh
authors.initials: M
authors.lastname: Mani
mesh_terms: D003430:Cross-Sectional Studies; D003967:Diarrhea / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests