title: Functional nodFE genes are present in Sinorhizobium sp. strain MUS10, a symbiont of the tropical legume Sesbania rostrata.
abstract: We have cloned the nodFE operon from Sinorhizobium sp. strain MUS10. MUS10 NodF shows sequence homology to acyl carrier protein and enables an S. meliloti nodF mutant to effectively nodulate alfalfa. Our results demonstrate the occurrence of nodFE in a symbiont that nodulates a legume host not belonging to the galegoid group.
authors.affiliation: Plant Genetics Research Unit, USDA-ARS, 108 Curtis Hall, University of Missouri, Columbia, MO 65211, USA. KrishnanH@missouri.edu
authors.forename: Hari B
authors.initials: HB
authors.lastname: Krishnan
authors.forename: Demosthenis
authors.initials: D
authors.lastname: Chronis
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D049931:Sesbania / Q000382:microbiology; D020579:Sinorhizobium / Q000235:genetics* / Q000502:physiology; D013559:Symbiosis
chemical_list: D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D004269:DNA, Bacterial