[Effect of Twist gene on the migration and invasion of gastric carcinoma cells].
OBJECTIVE
To explore the effect of Twist gene on the migration and invasion of human gastric carcinoma cells.


METHODS
MKN28 cells, a human gastric carcinoma cell line, were transfected with PcDNA3.1-Twist plasmid by lipofectamine transfecting technique. The transfected cells were selected with geneticin. Expressions of Twist,ecadherin and vimentin protein were detected by Western blot in cells transfected Twist gene. Matrigel invision chambers were performed to analyse the cell migration and invasion.


RESULTS
MKN28 cells transfected with PcDNA3.1-Twist plasmid showed stronger intracellular expression of Twist protein than MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. The expression of ecadherin protein in MKN28 cells transfected with PcDNA3.1-Twist plasmid was significantly decreased compared with that in MKN28 cells transfected with PcDNA3.1 and MKN28 cells without the transfection. However, The expression of vimentin protein in MKN28 cells transfected with PcDNA3.1-Twist plasmid was significantly increased compared with that in MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection. The migration and invasion ability of Twist+ - MKN28 cells were stronger than that of MKN28 cells transfected with PcDNA3.1 and MKN28 cells without transfection.


CONCLUSIONS
Twist gene may promote the migration and invasion ability of gastric carcinoma cells through epithelial mesenchymal transition.
Luo
Geng-qiu
GQ
Department of Pathology, School of Basic Medicine, Central South University, Changsha, China.
Wen
Ji-fang
JF
Li
Jing-he
JH
Hu
Yong-bin
YB
Zheng
Chang-li
CL
Jiang
Hai-ying
HY
D015820:Cadherins / Q000096:biosynthesis; D002465:Cell Movement / Q000235:genetics*; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D051879:Twist-Related Protein 1 / Q000096:biosynthesis / Q000235:genetics*; D014746:Vimentin / Q000096:biosynthesis
D015820:Cadherins; D051879:Twist-Related Protein 1; D014746:Vimentin