Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication, de-novo protein synthesis and caspase activation.
Newcastle disease virus causes (NDV) apoptotic death of infected cells. In the present study, the stimulus that provoked the induction of apoptosis in infected cells was examined. Vero cells infected with NDV developed apoptosis as characterized by DNA fragmentation and decreased DNA content. In presence of ammonium chloride, infected cells did not show reduced DNA content indicating the requirement of virus entry for the induction of apoptosis. UV-inactivated NDV did not induce apoptosis in cells suggesting the need of virus replication. Although cycloheximide blocked NDV-induced apoptosis, actinomycin-D did not, suggesting that de-novo viral protein synthesis was critical for the induction of apoptosis. In addition, activation of caspases was also detected by flowcytometry, indirect fluorescent and colorimetric assays. Based on the results, it was concluded that NDV-induced apoptosis in Vero cells required virus replication, de-novo protein synthesis and caspase activation.
Ravindra
P V
PV
Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India.
Tiwari
Ashok K
AK
Ratta
Barkha
B
Chaturvedi
Uttara
U
Palia
Sudesh Kumar
SK
Subudhi
Prasant Kumar
PK
Kumar
Rajiv
R
Sharma
Bhaskar
B
Rai
Anant
A
Chauhan
R S
RS
D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism*; D002522:Chlorocebus aethiops; D053938:DNA Fragmentation; D004789:Enzyme Activation; D009522:Newcastle disease virus / Q000472:pathogenicity* / Q000502:physiology; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D014764:Viral Proteins; D020169:Caspases