Influenza virus and proteolytic bacteria co-infection in respiratory tract from individuals presenting respiratory manifestations.
A role for proteolytic bacteria in the exacerbation of influenza virus has been shown in natural hosts such as pigs and humans. Four hundred seven samples were collected from the respiratory tract of individuals presenting clinical manifestations, during influenza season (2003-2005) in São Paulo City. The aim of this study was to evaluate the incidence of determined bacteria co-infecting virus in human respiratory tract. Tests, such as bacteriological, immunofluorescence (IF), RT/PCR and hemagglutination (HA) were used for bacterial and viral investigation. Thirty seven (9.09%) positive for influenza virus were screened by IF. The RT/PCR confirmed the presence of influenza virus in these samples. Bacterial and agar casein tests demonstrated that 18 (48.64%) individuals were infected with proteolytic bacteria such as Staphylococcus spp., Streptococcus spp. and Pseudomonas spp. Among these samples, 13 (35.13%) were co-infected with influenza A virus. Influenza type B, co-infecting bacteria were found in five (13.51%) samples. In vitro the S. aureus protease increased the influenza HA titer after contact for 30 min at 25 masculineC. Results revealed the occurrence of co-infection with proteolytic bacteria and influenza in the evaluated individuals. This finding corroborates that virus versus bacteria synergism could be able to potentiate respiratory infection, increasing damage to hosts.
Mancini
Dalva Assunção Portari
DA
Laboratório de Virologia, Instituto Butantan, São Paulo, SP, Brazil.
Alves
Rosely C Barbosa
RC
Mendonça
Rita Maria Zucatelli
RM
Bellei
Nancy J
NJ
Carraro
Emerson
E
Machado
Antonia M O
AM
Pinto
José Ricardo
JR
Mancini Filho
Jorge
J
D000293:Adolescent; D000328:Adult; D001424:Bacterial Infections / Q000150:complications* / Q000382:microbiology; D002648:Child; D005455:Fluorescent Antibody Technique; D006384:Hemagglutination; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D009981:Influenza B virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000150:complications / Q000382:microbiology / Q000821:virology*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013210:Staphylococcus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D013291:Streptococcus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D014775:Virus Activation
D012367:RNA, Viral