Proteomic analysis of microsomes from lactating bovine mammary gland.
Mammary gland has multiple metabolic potential including for large-scale synthesis of milk proteins, carbohydrate, and lipids including nutrient triacylglycerols. We have carried out a proteomic analysis of mammary tissue to discover proteins that affect lipid metabolism. Unfractionated microsomes from lactating bovine mammary tissue were analyzed using one-dimensional SDS-PAGE with RPLC-ESI-MS/MS. This approach gave 703 proteins including 160 predicted transmembrane proteins. Proteins were classified according to their subcellular localizations and biological functions. Over 50 proteins were associated with cellular uptake, metabolism, and secretion of lipids, including some enzymes that have been previously associated with breast cancer and potential therapeutic targets. This database develops a proteomic view of the metabolic potential of mammary gland that can be expected to contribute to a greater understanding of gene expression and tissue remodeling associated with lactation, and to further dissection of normal and pathological processes in mammary tissue.
Peng
Lifeng
L
Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
Rawson
Pisana
P
McLauchlan
Danyl
D
Lehnert
Klaus
K
Snell
Russell
R
Jordan
T William
TW
D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000032:analysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007774:Lactation / Q000378:metabolism*; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
D002352:Carrier Proteins; D020543:Proteome