title: Nucleoprotein structure of the CD4 locus: implications for the mechanisms underlying CD4 regulation during T cell development.
abstract: The CD4 gene is regulated in a stage-specific manner during T cell development, being repressed in CD4(-)CD8(-) double-negative (DN) and CD8 cells, but expressed in CD4(+)CD8(+) double-positive (DP) and CD4 cells. Furthermore, the expression/repression pattern is reversible in developing (DN and DP) thymocytes, but irreversible in mature (CD4 and CD8) T cells. Here, we explored the molecular mechanisms underlying this complex mode of regulation by examining the nucleoprotein structure of the CD4 locus throughout T cell development and in DN cells lacking the CD4 silencer. In DN cells, the CD4 enhancer is preloaded with multiple transcription activators, but p300 recruitment is impaired by the silencer that is associated with the repressor Runx1. DP cells achieve high-level CD4 expression via a combination of CD4 derepression and true activation, but Runx1 remains bound to the silencer that retains an open chromatin configuration. In CD4 cells, Runx1 dissociates from the silencer that has become less accessible, and CD4 transcription appears to be achieved via a mechanism distinct from that operating in DP cells. In CD8 cells, the CD4 promoter becomes incorporated into heterochromatin-like structure. Our data shed light on the molecular basis of CD4 regulation and provide a conceptual framework for understanding how the same regulatory elements can mediate both reversible and irreversible CD4 regulation.
authors.affiliation: Department of Immunobiology, Yale University Medical School, New Haven, CT 06520, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Mimi
authors.initials: M
authors.lastname: Wan
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Rohini
authors.initials: R
authors.lastname: Khatri
authors.forename: Tian
authors.initials: T
authors.lastname: Chi
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D004742:Enhancer Elements, Genetic / Q000235:genetics; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009698:Nucleoproteins / Q000737:chemistry*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D037941:Silencer Elements, Transcriptional / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D006657:Histones; D009698:Nucleoproteins; D014157:Transcription Factors