title: R5 and X4 HIV viruses differentially modulate host gene expression in resting CD4+ T cells.
abstract: During HIV-1 infection, distinct biological phenotypes are observed between R5 and X4 HIV-1 strains with respect to pathogenicity and tropism. In this study, temporal changes of the expression levels of the complete human transcriptome, representing 47,000 well-characterized human transcripts, were monitored in the first 24 h during HIV-1 R5 and X4 exposition in resting primary CD4(+) T cells. We provide evidence that R5 viruses modulate, to a greater extent than X4 viruses, the level of mRNA of the resting CD4(+) T cells. Indeed, modulation of the TCR signaling and the actin organization involving the WAVE/ABI complex and the ARP2/3 complex appeared to be associated with R5 exposition. The data suggest that the ability of R5 viruses to modulate TCR-mediated actin polymerization and signaling creates a favorable environment for CD4(+) T cell activation after TCR stimulation and may partly explain why R5 is the primary strain observed early in the natural infection process.
authors.affiliation: Infectious Disease Research Center, CHUL Research Center and Laval University, Québec, Canada. melissa.sirois@crchul.ulaval.ca
authors.forename: Mélissa
authors.initials: M
authors.lastname: Sirois
authors.forename: Lynda
authors.initials: L
authors.lastname: Robitaille
authors.forename: Roman
authors.initials: R
authors.lastname: Sasik
authors.forename: Jérôme
authors.initials: J
authors.lastname: Estaquier
authors.forename: Jessyka
authors.initials: J
authors.lastname: Fortin
authors.forename: Jacques
authors.initials: J
authors.lastname: Corbeil
mesh_terms: D051376:Actin-Related Protein 2-3 Complex / Q000032:analysis; D000199:Actins / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology* / Q000821:virology*; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D009219:Myosin-Light-Chain Kinase / Q000032:analysis; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000032:analysis
chemical_list: D051376:Actin-Related Protein 2-3 Complex; D000199:Actins; D017252:CD3 Complex; C115335:CD3E protein, human; D020543:Proteome; C120152:WASF2 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family; D009219:Myosin-Light-Chain Kinase