title: Superparamagnetic iron oxide nanoparticles coated with galactose-carrying polymer for hepatocyte targeting.
abstract: Our goal is to develop the functionalized superparamagnetic iron oxide nanoparticles (SPIONs) demonstrating the capacities to be delivered in liver specifically and to be dispersed in physiological environment stably. For this purpose, SPIONs were coated with polyvinylbenzyl-O-beta-D-galactopyranosyl-D-gluconamide (PVLA) having galactose moieties to be recognized by asialoglycoprotein receptors (ASGP-R) on hepatocytes. For use as a control, we also prepared SPIONs coordinated with 2-pyrrolidone. The sizes, size distribution, structure, and coating of the nanoparticles were characterized by transmission electron microscopy (TEM), electrophoretic light scattering spectrophotometer (ELS), X-ray diffractometer (XRD), and Fourier transform infrared (FT-IR), respectively. Intracellular uptake of the PVLA-coated SPIONs was visualized by confocal laser scanning microscopy, and their hepatocyte-specific delivery was also investigated through magnetic resonance (MR) images of rat liver. MRI experimental results indicated that the PVLA-coated SPIONs possess the more specific accumulation property in liver compared with control, which suggests their potential utility as liver-targeting MRI contrast agent.
authors.affiliation: School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, South Korea.
authors.forename: Mi Kyong
authors.initials: MK
authors.lastname: Yoo
authors.forename: In Yong
authors.initials: IY
authors.lastname: Kim
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Kim
authors.forename: Hwan-Jeong
authors.initials: HJ
authors.lastname: Jeong
authors.forename: Chang-Moon
authors.initials: CM
authors.lastname: Lee
authors.forename: Yong Yeon
authors.initials: YY
authors.lastname: Jeong
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Chong Su
authors.initials: CS
authors.lastname: Cho