Screening of Thai medicinal plants for anticandidal activity.
Medicinal plants are often used in the treatment of various ailments. In this study, 23 of Thai medicinal plants were screened for their anticandidal activity against six pathogenic Candida species: C. albicans, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis and C. tropicalis. The methanol extract of Hibiscus sabdariffa L. fruit, Trigonostemon reidioides (Kurz) Craib root, Usnea siamensis Vain whole plant, Boesenbergia rotunda (L.) Mansf. rhizome, and Albizia myriophylla Benth. stem showed anticandidal activity against one or more species of Candida. Among them, A. myriophylla Benth. showed broad anticandidal activity. The susceptibility tests of A. myriophylla Benth. extract, in terms of minimal inhibitory concentrations (MIC) and minimal fungicidal concentration (MFC), were performed by the broth microdilution techniques as described by the Clinical Laboratory Standard Institute. MICs of A. myriophylla Benth. extract to all Candida species was ranged 100-500 mug ml(-1). The killing activity of A. myriophylla Benth. extract was fast acting against all Candida tested; the reduction in the number of CFU ml(-1) was >3 log(10) units (99.9%) in 2 h. This study indicates that A. myriophylla Benth. extract has considerable anticandidal activity, deserving further investigation for clinical applications for the treatment of candidiasis.
Rukayadi
Yaya
Y
Department of Biotechnology, College of Engineering, Yonsei University, Seoul, Korea.
Shim
Jae-Seok
JS
Hwang
Jae-Kwan
JK
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D015169:Colony Count, Microbial; D004353:Drug Evaluation, Preclinical; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D013785:Thailand
D000935:Antifungal Agents; D010936:Plant Extracts