title: The role of loops 3 and 4 in the interdomains and intersubunits communication of E. coli cAMP receptor protein.
abstract: Cyclic AMP serves as an intracellular messenger in cells and regulates a variety of biological functions by transmitting information through proteins. These proteins of different functions all consist of a cAMP-binding motif, and the structure of this motif is highly conserved with an exception of the loop 3 and 4. In current study, cAMP receptor protein was employed as a model system to investigate the function of the two loops. The results indicated that the loop 3 involves in the intersubunits communication of CRP, whereas the loop 4 involves in cAMP binding and interdomains communication.
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors.forename: Xianrong
authors.initials: X
authors.lastname: Shen
authors.forename: Wenbing
authors.initials: W
authors.lastname: Tao
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
authors.forename: Shaoning
authors.initials: S
authors.lastname: Yu
mesh_terms: D020816:Amino Acid Motifs; D016880:Anisotropy; D000242:Cyclic AMP / Q000737:chemistry; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013816:Thermodynamics
chemical_list: D002373:Cyclic AMP Receptor Protein; D008024:Ligands; D004247:DNA; D000242:Cyclic AMP