High-Level folate production in fermented foods by the B12 producer Lactobacillus reuteri JCM1112.
We observed that Lactobacillus reuteri JCM1112 produces B(12) and folate. However, the folate/B(12) mass ratio found was far below that desired for human consumption ( approximately 170:1). We used metabolic engineering applying genetic and physiological approaches to improve this ratio and developed a generic and natural process that significantly increases folate production.
Santos
Filipe
F
Kluyver Centre for Genomics of Industrial Fermentation, TI Food and Nutrition, and NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.
Wegkamp
Arno
A
de Vos
Willem M
WM
Smid
Eddy J
EJ
Hugenholtz
Jeroen
J
D018533:Biomass; D005492:Folic Acid / Q000096:biosynthesis*; D005516:Food Microbiology; D052200:Limosilactobacillus reuteri / Q000235:genetics / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008954:Models, Biological; D014805:Vitamin B 12 / Q000096:biosynthesis
D005492:Folic Acid; D014805:Vitamin B 12