title: Quantification methods of folpet degradation products in plasma with HPLC-UV/DAD: application to an in vivo toxicokinetic study in rats.
abstract: Solid-phase extractions followed by HPLC-UV/DAD methods were developed for occupational biological monitoring or forensic investigations of the fungicide folpet using its degradation products, phthalimide and phthalamic acid as plasma biomarkers. These methods show good linearity (r>0.9955), precision (CV<15%) and accuracy (bias<14.8%). The lower limits of quantification for phthalimide and phthalamic acid were 10 and 20 ng/ml and the absolute recoveries were higher than 86% and 68%, respectively. Applying these methods, a plasma toxicokinetic study of folpet in rats after intratracheal administration of Folpan 80WG showed that inhalation of folpet could be a route of exposure with an important systemic absorption.
authors.affiliation: Departement de Pharmacologie, INSERM U657, Université Victor Segalen Bordeaux 2, 33076 Bordeaux, France. mireille.canal@pharmaco.u-bordeaux2.fr
authors.forename: Mireille
authors.initials: M
authors.lastname: Canal-Raffin
authors.forename: Mathilde
authors.initials: M
authors.lastname: Receveur
authors.forename: Béatrice
authors.initials: B
authors.lastname: Martinez
authors.forename: Karine
authors.initials: K
authors.lastname: Titier
authors.forename: Celine
authors.initials: C
authors.lastname: Ohayon
authors.forename: Isabelle
authors.initials: I
authors.lastname: Baldi
authors.forename: Mathieu
authors.initials: M
authors.lastname: Molimard
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
authors.forename: Patrick
authors.initials: P
authors.lastname: Brochard
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010797:Phthalimides / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D010797:Phthalimides; C005020:folpet