title: Identification of differentially expressed genes in mandibular condylar and tibial growth cartilages using laser microdissection and fluorescent differential display: chondromodulin-I (ChM-1) and tenomodulin (TeM) are differentially expressed in mandibular condylar and other growth cartilages.
abstract: Mandibular condylar cartilage can be distinguished from articular and growth cartilages of long bones based on several differences in morphology, physiology, and function between these structures. However, there is almost no information available on the types of genes that contribute to these differences. In this study, genes that were differentially expressed in mandibular condylar and growth cartilages in 1-week-old rats were investigated using fluorescent differential display (FDD) and laser microdissection (LMD). A number of genes were identified by FDD including chondromodulin-1 (ChM-1), which is known to be an angiogenesis inhibitor of endochondral ossification. ChM-1 expression was then compared with that of tenomodulin (TeM) in mandibular condylar and tibial cartilages of 1- and 5-week-old rats using real time PCR (RT-PCR), immunohistochemistry, and in situ hybridization. There was negligible detection of ChM-1 mRNA and protein in mandibular condylar cartilages compared to tibial cartilages of 1- and 5-week-old rats. On the other hand, TeM mRNA was more abundant in mandibular condylar cartilage than in tibial. These observations demonstrated that gene expression in mandibular condylar cartilage differed from other types of cartilage such as articular and growth ones.
authors.affiliation: Department of Orthodontics, School of Dentistry, Showa University, 2-1-1 kitasenzoku, Ohta-ku, Tokyo, 145-8515, Japan. junwata2000@ybb.ne.jp
authors.forename: Junichi
authors.initials: J
authors.lastname: Watahiki
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Akiko
authors.initials: A
authors.lastname: Enomoto
authors.forename: Tarou
authors.initials: T
authors.lastname: Irie
authors.forename: Kumi
authors.initials: K
authors.lastname: Yoshie
authors.forename: Tetsuhiko
authors.initials: T
authors.lastname: Tachikawa
authors.forename: Koutaro
authors.initials: K
authors.lastname: Maki
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000033:anatomy & histology / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D007834:Lasers; D008297:Male; D008335:Mandibular Condyle / Q000033:anatomy & histology* / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D042282:Microdissection / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000033:anatomy & histology* / Q000502:physiology
chemical_list: C489407:Cnmd protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C486027:Tnmd protein, rat