Over-expression of genes coding for proline oxidase, riboflavin kinase, cytochrome c oxidase and an MFS transporter induced by acriflavin in Trichophyton rubrum.
Acriflavin (3,6-acridinediamine) and other acridine derivatives act in both prokaryotic and eukaryotic cells at the level of DNA-coiling enzymes (topoisomerases) causing the stabilization of the enzyme-DNA cleavable complex. In order to better understand the mode of action of acriflavin, Differential Display RT-PCR was used to isolate transcripts specifically over-expressed during exposure of Trichophyton rubrum mycelia to this drug. Five transcripts, whose differential expressions were confirmed by Northern blotting, revealed genes not previously described in this dermatophyte. Functional grouping identified putative enzymes possibly involved in the mitochondrial respiratory electron-transport chain and in iron transport. These results may be relevant to our understanding of the molecular events involved in the stress response of T. rubrum to acriflavin.
Segato
Fernando
F
Departamento de Genética, USP, Ribeirão Preto, SP, Brazil.
Nozawa
Sérgio R
SR
Rossi
Antonio
A
Martinez-Rossi
Nilce M
NM
D000167:Acriflavine / Q000378:metabolism / Q000494:pharmacology*; D042967:Electron Transport Complex I; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D011394:Proline Oxidase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014249:Trichophyton / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
D026901:Membrane Transport Proteins; D000167:Acriflavine; D011394:Proline Oxidase; D003576:Electron Transport Complex IV; D017853:Phosphotransferases (Alcohol Group Acceptor); C022532:riboflavin kinase; D042967:Electron Transport Complex I