title: [Establishment and evaluation of the diagnostic kit for anti-HIV1/2 antibody and P24 antigen].
abstract: OBJECTIVE
  To establish and evaluate an Enzyme Immunoassay diagnostic kit combined with anti-HIV1/2 antibody and P24 antigen for shortening the examination window period of HIV infection in HIV laboratory diagnosis.
  
  
  METHODS
  The enzyme-linked reaction plates was coated by anti-HIV P24 monoclonal antibody and HIV 1/2 antigen. Labeling HIV1/2 antigen and anti-HIV P24 polyclonal antibody with horseradish peroxidase, setup an integrated ELISA kit for detecting anti-HIV-1/2 antibody and HIV P24 antigen, and evaluate the specificity and sensitivity of this kit.
  
  
  RESULTS
  The sensitivity of testing P24 antigen was up to 0.2 ng/ml. 78 serum samples of patients with AIDS, 85 serum samples of healthy people were compared with Abbott EIA kit, the coincidence was 100%. 12 051 sera from normal persons and patients were examined, the sensitivity of 100 %and specificity of 99.62 %, respectively.
  
  
  CONCLUSIONS
  The anti-HIV1/2 antibody and HIV P24 antigen can be measured at the same time using this EIA kit, while the examination window period of HIV infection is shortened. Thus, the method is suitable for laboratory diagnosis and epidemiological investigation.
authors.affiliation: Department of Virology, 302nd Hospital of PLA, Beijing, 100039, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Feng
authors.forename: Chang-fang
authors.initials: CF
authors.lastname: Feng
authors.forename: Su-juan
authors.initials: SJ
authors.lastname: Shi
authors.forename: Hong-hui H
authors.initials: HH
authors.lastname: Shen
authors.forename: Zhi-jie
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Bao-jun
authors.initials: BJ
authors.lastname: Wang
authors.forename: Pan-yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood*; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D011933:Reagent Kits, Diagnostic
chemical_list: D015483:HIV Antibodies; D016655:HIV Core Protein p24; D011933:Reagent Kits, Diagnostic