Role of CheY1 and CheY2 in the chemotaxis of A. tumefaciens toward acetosyringone.
Agrobacterium tumefaciens has a chemtaxis operon, which includes orf1, orf2, cheY1, cheA, cheR, cheB, cheY2, orf9, and orf10. In-frame deletions of cheY1 and cheY2 were constructed and the chemosensory behavior of the mutants was examined on swarm plates and in a chemotaxis assay toward acetosyringone. The cheY2 mutant (C1/delY2) showed impaired chemotactic capabilities in both swarming and chemotaxis assays. The effect of lacking CheY1 on chemotaxis is less severe than that of CheY2, under the conditions studied.
Harighi
Behrouz
B
Department of Plant Protection, College of Agriculture & Natural Resources, University of Kurdistan, Sanandaj, Iran. BHarighi@uok.ac.ir
D000098:Acetophenones / Q000494:pharmacology*; D016960:Agrobacterium tumefaciens / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis; D009876:Operon; D017384:Sequence Deletion
D000098:Acetophenones; D001426:Bacterial Proteins; C051667:acetosyringone