title: Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication, de-novo protein synthesis and caspase activation.
abstract: Newcastle disease virus causes (NDV) apoptotic death of infected cells. In the present study, the stimulus that provoked the induction of apoptosis in infected cells was examined. Vero cells infected with NDV developed apoptosis as characterized by DNA fragmentation and decreased DNA content. In presence of ammonium chloride, infected cells did not show reduced DNA content indicating the requirement of virus entry for the induction of apoptosis. UV-inactivated NDV did not induce apoptosis in cells suggesting the need of virus replication. Although cycloheximide blocked NDV-induced apoptosis, actinomycin-D did not, suggesting that de-novo viral protein synthesis was critical for the induction of apoptosis. In addition, activation of caspases was also detected by flowcytometry, indirect fluorescent and colorimetric assays. Based on the results, it was concluded that NDV-induced apoptosis in Vero cells required virus replication, de-novo protein synthesis and caspase activation.
authors.affiliation: Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar 243 122, UP, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Ravindra
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Tiwari
authors.forename: Barkha
authors.initials: B
authors.lastname: Ratta
authors.forename: Uttara
authors.initials: U
authors.lastname: Chaturvedi
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Palia
authors.forename: Prasant Kumar
authors.initials: PK
authors.lastname: Subudhi
authors.forename: Rajiv
authors.initials: R
authors.lastname: Kumar
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Sharma
authors.forename: Anant
authors.initials: A
authors.lastname: Rai
authors.forename: R S
authors.initials: RS
authors.lastname: Chauhan
mesh_terms: D000818:Animals; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism*; D002522:Chlorocebus aethiops; D053938:DNA Fragmentation; D004789:Enzyme Activation; D009522:Newcastle disease virus / Q000472:pathogenicity* / Q000502:physiology; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D020169:Caspases