[Studies on genetic diversity of medicinal Dendrobium by SRAP].
OBJECTIVE
To study the genetic diversity of medicinal Dendrobium by SRAP.


METHODS
The genetic diversity of 9 spices Dendrobium was studied by using the optimized SRAP reaction system. The NTSYS software was used to analyze the markers.


RESULTS
Forty primer pairs were selected from 88 amplified 1 782 polymorphic bands with an average of 44.55 polymorphic bands per primer pair. Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 40 primer pairs showed that 9 spices of could be distinguished into two main groups. Jaccard's similarity coefficient ranged from 0.330 2-0.789 2.


CONCLUSIONS
The results of this research indicate that SRAP molecular marker is efficient to study the medical Dendrobium genetic diversity.
Fan
Hong-Hong
HH
School of Life Science, Anhui Agriculture University, Heifei 230036, China.
Li
Ting-Chun
TC
Qiu
Jing
J
Li
Zheng-Peng
ZP
Lin
Yi
Y
Cai
Yong-Ping
YP
D031667:Dendrobium / Q000145:classification* / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction