title: Over-expression of genes coding for proline oxidase, riboflavin kinase, cytochrome c oxidase and an MFS transporter induced by acriflavin in Trichophyton rubrum.
abstract: Acriflavin (3,6-acridinediamine) and other acridine derivatives act in both prokaryotic and eukaryotic cells at the level of DNA-coiling enzymes (topoisomerases) causing the stabilization of the enzyme-DNA cleavable complex. In order to better understand the mode of action of acriflavin, Differential Display RT-PCR was used to isolate transcripts specifically over-expressed during exposure of Trichophyton rubrum mycelia to this drug. Five transcripts, whose differential expressions were confirmed by Northern blotting, revealed genes not previously described in this dermatophyte. Functional grouping identified putative enzymes possibly involved in the mitochondrial respiratory electron-transport chain and in iron transport. These results may be relevant to our understanding of the molecular events involved in the stress response of T. rubrum to acriflavin.
authors.affiliation: Departamento de Genética, USP, Ribeirão Preto, SP, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Segato
authors.forename: Sérgio R
authors.initials: SR
authors.lastname: Nozawa
authors.forename: Antonio
authors.initials: A
authors.lastname: Rossi
authors.forename: Nilce M
authors.initials: NM
authors.lastname: Martinez-Rossi
mesh_terms: D000167:Acriflavine / Q000378:metabolism / Q000494:pharmacology*; D042967:Electron Transport Complex I; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D011394:Proline Oxidase / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014249:Trichophyton / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D000167:Acriflavine; D011394:Proline Oxidase; D003576:Electron Transport Complex IV; D017853:Phosphotransferases (Alcohol Group Acceptor); C022532:riboflavin kinase; D042967:Electron Transport Complex I