title: Increased basolateral sorting of carcinoembryonic antigen in a polarized colon carcinoma cell line after cholesterol depletion-Implications for treatment of inflammatory bowel disease.
abstract: OBJECTIVE
  To investigate a possible increase of basolateral expression of carcinoembryonic antigen (CEA) by interfering with the apical transport machinery, we studied the effect of cholesterol depletion on CEA sorting and secretion.
  
  
  METHODS
  Cholesterol depletion was performed in polarized Caco-2 cells using lovastatin and methyl-beta-cyclodextrin.
  
  
  RESULTS
  We show that CEA is predominantly expressed and secreted at the apical surface. Reduction of the cholesterol level of the cell by 40%-50% with lovastatin and methyl-beta-cyclodextrin led to a significant change of the apical-to-basolateral transport ratio towards the basolateral membrane.
  
  
  CONCLUSIONS
  As basolateral expression of CEA has been suggested to have anti-inflamatory properties, Cholesterol depletion of enterocytes might be a potential approach to influence the course of inflammatory bowel disease.
authors.affiliation: Department of Internal Medicine IV University Hospital, INF410, Heidelberg 69120, Germany. robert_ehehalt@urz.uni-heidelberg.de
authors.forename: Robert
authors.initials: R
authors.lastname: Ehehalt
authors.forename: Markus
authors.initials: M
authors.lastname: Krautter
authors.forename: Martin
authors.initials: M
authors.lastname: Zorn
authors.forename: Richard
authors.initials: R
authors.lastname: Sparla
authors.forename: Joachim
authors.initials: J
authors.lastname: Fullekrug
authors.forename: Hasan
authors.initials: H
authors.lastname: Kulaksiz
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Stremmel
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000924:Anticholesteremic Agents / Q000627:therapeutic use*; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D018938:Caco-2 Cells; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000378:metabolism; D008148:Lovastatin / Q000627:therapeutic use; D047392:beta-Cyclodextrins / Q000627:therapeutic use*
chemical_list: D000924:Anticholesteremic Agents; D002272:Carcinoembryonic Antigen; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002784:Cholesterol; D008148:Lovastatin