title: Application of the Sleeping Beauty transposon system to avian cells.
abstract: We have for the first time assessed the ability of the Sleeping Beauty (SB) transposon system to enhance transgenesis in chicken and turkey cells. The efficiency of transgenesis with a transposon encoding an antibiotic resistance gene was dramatically enhanced 15- to 35-fold when transposase was supplied by co-transfection of immortalized chicken and turkey cells with a construct encoding SB. In contrast, transgenesis of primary chicken embryo fibroblast (CEF) cells was not significantly increased by providing transposase, suggesting that the benefits of transposon-transgenesis in primary avian cells will require the application of more efficient transfection methods, further enhanced SB transposase or an alternative transposon system.
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: B-W
authors.initials: BW
authors.lastname: Kong
authors.forename: D F
authors.initials: DF
authors.lastname: Carlson
authors.forename: S C
authors.initials: SC
authors.lastname: Fahrenkrug
authors.forename: D N
authors.initials: DN
authors.lastname: Foster
mesh_terms: D000123:Acetyltransferases / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D018929:Cell Culture Techniques; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005347:Fibroblasts / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D014162:Transfection; D014422:Turkeys / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D000123:Acetyltransferases; C045336:puromycin N-acetyltransferase