Peptide-15 changes miRNA expression in osteoblast-like cells.
OBJECTIVE
Peptide-15 (P-15) is an analog of the cell-binding domain of collagen. P-15 has been shown to facilitate physiological process in a way similar to collagen, to serve as anchorage for cells, and to promote the binding, migration, and differentiation of cells. However, how P-15 alters osteoblast activity to promote bone formation is poorly understood. We therefore attempted to address this question by using microarray techniques to investigate the microRNA (miRNA) expression in osteoblasts exposed to P-15.


METHODS
The miRNA oligonucleotide microarray provides a novel method to carry out genome-wide miRNA profiling in human samples. By using miRNA microarrays containing 329 probe designed from human miRNA sequence, we identified in osteoblast-like cells line (MG-63) cul-tured with P-15 several miRNA whose expression is significantly modified.


RESULTS
We identified 11 up-regulated miRNA (i.e., mir-337, mir-15b, mir-377, mir-100, mir-148a, mir-125a, mir-199a, mir-221, mir-let-7d, mir-92, mir-23b) and six down-regulated miRNA (i.e., mir-422a, mir-19a, mir-224, mir-145, mir-22, mir-29a).


CONCLUSIONS
The data reported are, to our knowledge, the first on translation regulation in osteoblasts exposed to P-15. They can be relevant to better understand the molecular mechanism of bone regeneration and can serve as a model for comparing other materials with similar clinical effects.
Palmieri
Annalisa
A
Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy.
Pezzetti
Furio
F
Brunelli
Giorgio
G
Martinelli
Marcella
M
Lo Muzio
Lorenzo
L
Scarano
Antonio
A
Degidi
Marco
M
Piattelli
Adriano
A
Carinci
Francesco
F
D002460:Cell Line; D003094:Collagen / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology*
D035683:MicroRNAs; D010446:Peptide Fragments; C407062:cell-binding peptide P-15; D003094:Collagen