On-virus construction of polyvalent glycan ligands for cell-surface receptors.
Glycans arrayed on the exterior of virus particles were used as substrates for glycosyltransferase reactions to build di- and trisaccharides from the virus surface. The resulting particles exhibited tight and specific associations with cognate receptors on beads and cells, in one example defeating in cis cell-surface interactions in a manner characteristic of polyvalent binding. Combined with the ability of viruses to provide structurally well-defined attachment points, the methodology provides a convenient and powerful way to prepare complex carbohydrate ligands for clustered receptors.
Kaltgrad
Eiton
E
Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
O'Reilly
Mary K
MK
Liao
Liang
L
Han
Shoufa
S
Paulson
James C
JC
Finn
M G
MG
D017908:Allolevivirus / Q000737:chemistry*; D002213:Capsid / Q000737:chemistry; D045744:Cell Line, Tumor; D017798:Comovirus / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000737:chemistry / Q000378:metabolism
D011134:Polysaccharides; D011956:Receptors, Cell Surface; D051918:Sialic Acid Binding Ig-like Lectin 2