Functional nodFE genes are present in Sinorhizobium sp. strain MUS10, a symbiont of the tropical legume Sesbania rostrata.
We have cloned the nodFE operon from Sinorhizobium sp. strain MUS10. MUS10 NodF shows sequence homology to acyl carrier protein and enables an S. meliloti nodF mutant to effectively nodulate alfalfa. Our results demonstrate the occurrence of nodFE in a symbiont that nodulates a legume host not belonging to the galegoid group.
Krishnan
Hari B
HB
Plant Genetics Research Unit, USDA-ARS, 108 Curtis Hall, University of Missouri, Columbia, MO 65211, USA. KrishnanH@missouri.edu
Chronis
Demosthenis
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D000213:Acyl Carrier Protein / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017353:Gene Deletion; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D049931:Sesbania / Q000382:microbiology; D020579:Sinorhizobium / Q000235:genetics* / Q000502:physiology; D013559:Symbiosis
D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D004269:DNA, Bacterial