title: [Neurons in the corpus callosum of rats: expression of Cav2.2 and their connection].
abstract: OBJECTIVE
  To prove the existence neurons in the rat corpus callosum, the potential function of these neurons and their connection.
  
  
  METHODS
  Immunohistochemistry was used performed to examine the expressions of NeuN, a mature neuron marker,and N-type voltage-dependent valcium channel alpha1-subunit (Cav2.2)in the section of the rat corpus callosum. Horseradish peroxidase (HRP) normal sodium solution (30%), the retrograde tracer,was injected under the frontal forceps of corpus callousm and HRP absorbed by the process of neurons in the brain slices was stained with tetramethyl benzidine.
  
  
  RESULTS
  There were some NeuN positive cells in the rat corpus callosum and Cav2.2 was detected in some of these NeuN positive cells.Neurons with positive HRP were found in the rat corpus callosum and some of these neurons connected to the cortex or corpus striatum.
  
  
  CONCLUSIONS
  There are a few neurons in the corpus callosum of adult rats and some of them express Cav2.2. Neurons in the corpus callosum have connections with the brain cortex or corpora striatum.
authors.affiliation: Institute of Neurobiology,School of Medicine, Xioan Jiaotong University, Xi'an, China.
authors.forename: Jian-shui
authors.initials: JS
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie-hua
authors.initials: JH
authors.lastname: Xu
authors.forename: Peng-bo
authors.initials: PB
authors.lastname: Yang
authors.forename: Xin-li
authors.initials: XL
authors.lastname: Xiao
authors.forename: Xin-lin
authors.initials: XL
authors.lastname: Chen
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Tian
authors.forename: Jun-feng
authors.initials: JF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020864:Calcium Channels, N-Type / Q000096:biosynthesis*; D003337:Corpus Callosum / Q000166:cytology / Q000378:metabolism*; D004268:DNA-Binding Proteins; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009434:Neural Pathways / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C503279:Cacna1b protein, rat; D020864:Calcium Channels, N-Type; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins