[The regulatory effect of protein kinase C epsilon on vascular reactivity and calcium sensitivity during hemorrhagic shock in rats].
OBJECTIVE
To observe the regulatory effect of protein kinase C epsilon (PKC epsilon) on vascular reactivity and calcium sensitivity following hemorrhagic shock.


METHODS
The superior mesenteric artery (SMA) obtained from rats in hemorrhagic shock for different time duration (immediately, 0.5 hour, 1 hour, 2 hours and 4 hours after shock) were adopted to determine the mRNA expression of PKC epsilon using reverse transcription-polymerase chain reaction (RT-PCR) technique, and the first branch of SMA were adopted to assay the vascular reactivity and calcium sensitivity by observing the contraction initiated by norepinephrine (NE) and Ca2+ with isolated organ perfusion system. The agonist and antagonist of PKC epsilon were used to observe the regulation of PKC epsilon on vascular reactivity and calcium sensitivity at 2 hours after hemorrhagic shock.


RESULTS
(1) Vascular reactivity and calcium sensitivity of the first branch of SMA were increased at the early stage of hemorrhagic shock, and decreased at the late stage of hemorrhagic shock. PKC epsilon mRNA exhibited a time-dependent increase following hemorrhagic shock, peaked at 1 hour (P<0.01) and maintained at a high level at 4 hours (P<0.01). (2) The agonist of PKC epsilon increased the vascular reactivity and calcium sensitivity of the first branch of SMA, and its antagonist decreased the vascular reactivity and calcium sensitivity of SMA at 2 hours after shock (all P<0.01).


CONCLUSIONS
PKC epsilon may be an important endogenous protective molecule. It plays an important role in the regulation of vascular reactivity and calcium sensitization following hemorrhagic shock.
Xu
Jing
J
State Key Laboratory of Trauma, Burns and Combined Injury, Department 2, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Yang
Guang-ming
GM
Li
Tao
T
Ming
Jia
J
Chen
Wei
W
Zhang
Yuan
Y
Liu
Liang-ming
LM
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D017538:Mesenteric Artery, Superior / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D051744:Protein Kinase C-epsilon / Q000378:metabolism / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012771:Shock, Hemorrhagic / Q000378:metabolism / Q000503:physiopathology*; D014661:Vasoconstriction / Q000187:drug effects
D051744:Protein Kinase C-epsilon; D002118:Calcium