title: Utility of separation science in metabolomic studies.
abstract: Metabolomics, or alternately metabonomics, an emerging field of biochemical research, is a complementary technique to genomics, transcriptomics, and proteomics. Direct quantitative measurements of metabolite expressions in urine, serum, plasma, and tissue are essential for the study of biological processes in normal and disease states. Since the number of metabolites in a biological sample is large separation science plays an important role in metabolomic research. This review describes the role of GC, HPLC, CE, and supercritical fluid chromatography in metabolomic research. The application of each technique is discussed and its advantages and limitations are discussed with selective illustrative examples.
authors.affiliation: Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD, USA. issaqh@mail.ncifcrf.gov
authors.forename: Haleem J
authors.initials: HJ
authors.lastname: Issaq
authors.forename: Eric
authors.initials: E
authors.lastname: Abbott
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Veenstra
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002623:Chemistry Techniques, Analytical; D002845:Chromatography; D006801:Humans; D013058:Mass Spectrometry; D008660:Metabolism
chemical_list: D015415:Biomarkers