Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2.
It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE(2). In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.
Chang
Chih-Hung
CH
Division of Orthopaedics, Department of Surgery, Far Eastern Memorial Hospital, Taipei, Taiwan.
Fang
Hsu-Wei
HW
Ho
Yi-Ching
YC
Huang
Huei-Ting
HT
D000818:Animals; D019643:Arthroplasty, Replacement / Q000009:adverse effects*; D002358:Cartilage, Articular / Q000166:cytology / Q000276:immunology* / Q000601:surgery; D019902:Chondrocytes / Q000276:immunology* / Q000648:ultrastructure; D018920:Coculture Techniques; D015232:Dinoprostone / Q000378:metabolism; D005434:Flow Cytometry; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism; D010003:Osteoarthritis / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology; D010587:Phagocytosis; D011095:Polyethylenes / Q000009:adverse effects* / Q000378:metabolism; D034421:Sus scrofa
D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene; D009569:Nitric Oxide; D015232:Dinoprostone