title: An entropic perspective of protein stability on surfaces.
abstract: The interaction of proteins with surfaces regulates numerous processes in nature, science, and technology. In many applications, it is desirable to place proteins on surfaces in an active state, and tethering represents one manner in which to accomplish this. However, a clear understanding of how tether placement and design affects protein activity is lacking. Available theoretical models predict that proteins will be stabilized when tethered to substrates. Such models suggest that the surface reduces the number of states accessible to the unfolded state of the protein, thereby reducing the entropic cost of folding on the surface compared to the bulk case. Recent studies, however, have shown that this stabilization is not always seen. The purpose of this article is to determine the validity of the theory with a thorough thermodynamic analysis of the folding of peptides attached to surfaces. Configuration-temperature-density-of-states Monte Carlo simulations are used to examine the behavior of four different peptides of different secondary and tertiary structure. It is found that the surface does reduce the entropic cost of folding for tethered peptides, as the theory suggests. This effect, however, does not always translate into improved stability because the surface may also have a destabilizing enthalpic effect. The theory neglects this effect and assumes that the enthalpy of folding is the same on and off the surface. Both the enthalpic and entropic contributions to the stability are found to be topology- and tether-placement-specific; we show that stability cannot be predicted a priori. A detailed analysis of the folding of protein A shows how the same protein can be both stabilized and destabilized on a surface depending upon how the tethering enhances or hinders the ability of the peptide to form correct tertiary structures.
authors.affiliation: Department of Chemical Engineering, Brigham Young University, Provo, Utah, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Knotts
authors.forename: Nitin
authors.initials: N
authors.lastname: Rathore
authors.forename: Juan J
authors.initials: JJ
authors.lastname: de Pablo
mesh_terms: D000327:Adsorption; D001665:Binding Sites; D003198:Computer Simulation; D019277:Entropy; D008956:Models, Chemical; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D013205:Staphylococcal Protein A