title: The particles of the embryonic cerebrospinal fluid: how could they influence brain development?
abstract: During brain development, the embryonic cerebrospinal fluid (E-CSF) allows brain expansion and promotes neuroepithelial cell survival, proliferation or differentiation. Previous analyses of E-CSF content have revealed a high protein concentration and the presence of membranous particles. The role of these particles in the E-CSF remains poorly investigated. In this study we showed that the E-CSF contains at least two pools of particles: lipoproteins and exosome-like particles. We showed that these two populations of particles strongly interact with neuropithelial cells via an endocytic process, which display regional specificity along the developing neural tube. Finally, we explore and discuss the possibility that these interactions may influence brain development through the regulation of morphogen and growth factor signaling transduction.
authors.affiliation: CNRS UMR 8542, Ecole Normale Supérieure, 75005 Paris, France. bachy@biologie.ens.fr
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bachy
authors.forename: Renata
authors.initials: R
authors.lastname: Kozyraki
authors.forename: Marion
authors.initials: M
authors.lastname: Wassef
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001054:Apolipoproteins A / Q000235:genetics / Q000378:metabolism; D001921:Brain / Q000187:drug effects* / Q000196:embryology / Q000648:ultrastructure; D002555:Cerebrospinal Fluid / Q000737:chemistry* / Q000378:metabolism; D002642:Chick Embryo; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009419:Nerve Tissue Proteins / Q000378:metabolism; D046569:Neuroepithelial Cells / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D052638:Particulate Matter / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000596:Amino Acids; D001054:Apolipoproteins A; D009419:Nerve Tissue Proteins; D052638:Particulate Matter; C098463:dolaisoleucine