title: Dose-related effects following oral exposure of 2,4-dinitrotoluene on the Western fence lizard, Sceloporus occidentalis.
abstract: 2,4-dintitrotoluene (2,4-DNT) is an explosive frequently found in the soil of military installations. Because reptiles can be common on these sites, ecological risk assessments for compounds such as 2,4-DNT could be improved with toxicity data specific to reptiles. Western fence lizards, Sceloporus occidentalis, were used to develop a laboratory toxicity model for reptiles. A hierarchical approach was used; acute to subchronic studies were conducted to provide toxicity data relevant to short- and long-term exposures. First, a modified median lethal dose (LD50) study was conducted on male and female lizards using a stage-wise probit model. The LD50 was 577 mg/kg for female and 380 mg/kg for male lizards. Subsequently, a subacute experiment was conducted to further assess 2,4-DNT toxicity to male lizards and to define exposure levels for a longer term, subchronic study. The subchronic study was conducted for 60 consecutive days; male lizards were exposed to 0, 9, 15, 25, 42, 70 mg/kg/d. Dose-dependent mortality was observed in the three highest dose groups (25, 42, and 70 mg/kg/d); all other animals survived the study duration. Benchmark dose model calculations based on mortality indicated a 5% effect level of 15.8 mg/kg/d. At study termination, a gross necropsy was performed, organ weights were taken, and blood was collected for clinical and hematological analysis. Body weight, kidney weight, food consumption, postdose observations, and blood chemistries all were found to be significantly different from controls at doses above 9 mg/kg/d. Also, preliminary results suggest behavioral observations, and reduced food consumption may be a sensitive indicator of toxicity. The present study indicates Sceloporus occidentalis is suitable for evaluating toxicity of compounds to reptilian species.
authors.affiliation: US Army Center for Health Promotion and Preventive Medicine, Aberdeen Proving Ground, MD 21010, USA. suski.jamie@epa.gov
authors.forename: Jamie G
authors.initials: JG
authors.lastname: Suski
authors.forename: Christopher
authors.initials: C
authors.lastname: Salice
authors.forename: John T
authors.initials: JT
authors.lastname: Houpt
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Bazar
authors.forename: Larry G
authors.initials: LG
authors.lastname: Talent
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004136:Dinitrobenzenes / Q000008:administration & dosage* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004785:Environmental Pollutants / Q000008:administration & dosage* / Q000494:pharmacology*; D007928:Lethal Dose 50; D008116:Lizards; D008297:Male
chemical_list: D004136:Dinitrobenzenes; D004785:Environmental Pollutants; C016403:2,4-dinitrotoluene