[Killing effect improved by fusion gene HRE1.Egr-1. yCDglyTK on gene-radio therapy of nasopharyngeal cancer in vitro].
OBJECTIVE
To construct hypoxia/radiation inducible promotor HRE1.Egr-1, and to observe its promotive effect on the expression of yCDglyTK gene in nasopharyngeal cancer HNE-1 cells and the anti-tumor effect of yCDglyTK and to lay an experimental foundation for further exploration of new gene-radio therapy of nasopharyngeal cancer.


METHODS
pcDNA3.1(-)HRE1.Egr-1.yCDglyTK was constructed by gene recombination technique. Stable yCDglyTK-expressing HNE-1 cells were generated by transfecting the recombinant plasmid into the target cells with liposome. The expression of yCDglyTK was detected by Western blot in 4 groups: a normoxia group, a radiation group, a hypoxia group, and a hypoxia and radiation group. The killing effect of 5-FC in different circumstances was determined by MTT.


RESULTS
The expression of yCDglyTK/5-FC gene in all the groups was significantly different(P<0.01),especially in the hypoxia and radiation group. The killing effect of 5-FC on HNE1 cells varied under different conditions, especially in the hypoxia and radiation group.


CONCLUSIONS
Hypoxia and radiation can induce the activity of fusion promoter HRE1.Egr-1, and obviously promote the anti-tumor effect of yCDglyTK/5-FC system, suggesting that yCDglyTK may be a candidate suicide gene for gene-radio therapy of NPC.
Zhong
Yu
Y
Department of Otolaryngology, Xiangya Hospital,Central South University, Changsha, China.
Tang
Yao-yun
YY
Xie
Chang-ning
CN
Zhao
Su-ping
SP
D051766:Early Growth Response Protein 1 / Q000235:genetics*; D005437:Flucytosine / Q000494:pharmacology; D050939:Gene Fusion / Q000502:physiology*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000532:radiotherapy / Q000628:therapy*; D020218:Response Elements / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D005437:Flucytosine; D013937:Thymidine Kinase