[Effect of C21 steroidal glycoside from root of Cynanchum auriculatum on D-galactose induced aging model mice].
OBJECTIVE
To study the effect of C21 steroidal glycoside (CSG) from the root of Cynanchum auriculatum from Jiangsu on D-galactose (D-gal) induced aging model mice.


METHODS
D-gal aging mouse model was established by cervicodorsal region subcutaneous injection with D-gal once a day for eight successive weeks. The mice in the normal control group (NCG, non-modeled) and the model control group (MCG, modeled but untreated) were treated with 1% CMC-Na. The model mice in the low, middle and high-dose CSG and Vitamin E treated groups were treated with a dose of (10, 20, 40, 100 mg x kg(-1) per day, respectively. The SOD activity, MDA content and telomerase activity in serum, heart, liver and brain tissues of mice were measured.


RESULTS
CSG could obviously increase the SOD activity and decrease the MDA level in serum, heart, liver and brain tissues in D-gal aging mice (P < 0.01). There was no significant difference between three CSG treated groups and Vitamin E treated groups. In comparison of telomerase activity between MCG and the treated groups, it was shown that there was a significant increase in serum in middle and high dose group, and in heart tissues in CSG and Vit E treated groups, but was not in liver and brain tissue.


CONCLUSIONS
This study demonstrates that CSG can antagonize free radical injury, increase the SOD activity and decrease the MDA content of serum, heart, liver and brain in D-gal aging mice, and increase the telomerase activity in serum and heart tissues but not in liver and brain tissue.
Zhang
Shi-Xia
SX
The Medical and Pharmaceutical Academy of Yangzhou University, Yangzhou 225001, China.
Li
Xin
X
Yin
Jia-Le
JL
Chen
Li-Li
LL
Zhang
Hong-Quan
HQ
D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D031105:Cynanchum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D005690:Galactose / Q000633:toxicity; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D009206:Myocardium / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D013256:Steroids / Q000302:isolation & purification / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D013256:Steroids; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D019098:Telomerase; D005690:Galactose