title: High-fat diet may impair K(ATP) channels in vascular smooth muscle cells.
abstract: K(ATP) channel in vascular smooth muscle cell (VSMC) is closely linked to the etiology of hypertension. The aim of this study was to investigate effect of the high-fat diet-induced obesity on K(ATP) channel and blood pressure. Obesity was induced by a 24-week high-fat diet feeding in rats. Function and expression of K(ATP) channel in mesenteric arteries were examined using myography system, patch clamp and Western blotting. We show that high-fat diet increased blood pressure, decreased K(ATP) channel-mediated relaxation responses and currents, and down-regulated K(ATP) expression in VSMC. In conclusion, diet-induced obesity impairs K(ATP) channels in VSMC, which may underscore obesity-triggered increase in blood pressure.
authors.affiliation: Department of Cardiology, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, PR China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Fan
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Tian
authors.forename: Miao-Li
authors.initials: ML
authors.lastname: Yang
authors.forename: Ai-Qun
authors.initials: AQ
authors.lastname: Ma
authors.forename: Zhi
authors.initials: Z
authors.lastname: Hu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Bai
authors.forename: Yong-Xiao
authors.initials: YX
authors.lastname: Cao
mesh_terms: D000818:Animals; D001794:Blood Pressure; D015153:Blotting, Western; D004041:Dietary Fats / Q000009:adverse effects*; D004195:Disease Models, Animal; D015536:Down-Regulation; D006973:Hypertension / Q000209:etiology; D054086:KATP Channels / Q000378:metabolism*; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D009213:Myography; D009765:Obesity / Q000209:etiology / Q000503:physiopathology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004041:Dietary Fats; D054086:KATP Channels