[Simultaneous determination of contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides by HPLC].
OBJECTIVE
To develop a HPLC method for simultaneous determination of the contents of sophoridine, oxymatrine and oxysophocarpine in seed of Sophora alopecuroides.


METHODS
Chromatographic condition included a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) column and thmobile phase consisting of a mixture of methanol-0.2% phosphatic acid (7:93). The detection wavelength was at 205 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.


RESULTS
All calibration curves showed good linearity (r > or = 0.9997) within test ranges. The mean recoveries were 100.0%, 97.0% and 97.0% respectively.


CONCLUSIONS
This method is simple, rapid and accurate, and suitable for quality control of S. alopecuroides seed.
Sabir
Gulnar
G
Xingjiang Institute of Chinese Material Medica and Ethica, Urumch 830002, China. gulnar_sabir@163.com
Aisa
Haji Akber
HA
Shi
Ming-Hui
MH
Jia
Xiao-Guang
XG
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
D000470:Alkaloids; D011807:Quinolizines; C046246:oxysophocarpine; C037573:oxymatrine; D000093842:Matrines