Sugar Chips immobilized with synthetic sulfated disaccharides of heparin/heparan sulfate partial structure.
Carbohydrate chip technology has a great potential for the high-throughput evaluation of carbohydrate-protein interactions. Herein, we report syntheses of novel sulfated oligosaccharides possessing heparin and heparan sulfate partial disaccharide structures, their immobilization on gold-coated chips to prepare array-type Sugar Chips, and evaluation of binding potencies of proteins by surface plasmon resonance (SPR) imaging technology. Sulfated oligosaccharides were efficiently synthesized from glucosamine and uronic acid moieties. Synthesized sulfated oligosaccharides were then easily immobilized on gold-coated chips using previously reported methods. The effectiveness of this analytical method was confirmed in binding experiments between the chips and heparin binding proteins, fibronectin and recombinant human von Willebrand factor A1 domain (rh-vWf-A1), where specific partial structures of heparin or heparan sulfate responsible for binding were identified.
Wakao
Masahiro
M
Department of Nanostructure and Advanced Materials, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 890-0065, Japan.
Saito
Akihiro
A
Ohishi
Koh
K
Kishimoto
Yuko
Y
Nishimura
Tomoaki
T
Sobel
Michael
M
Suda
Yasuo
Y
D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry*; D006801:Humans; D008956:Models, Chemical; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013431:Sulfates / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods
D004187:Disaccharides; D005353:Fibronectins; D006046:Gold; D006493:Heparin; D006497:Heparan Sulfate; D009844:Oligosaccharides; D011994:Recombinant Proteins; D013431:Sulfates