title: Association of serum and mucosal neutralizing antibodies to human papillomavirus type 16 (HPV-16) with HPV-16 infection and cervical disease.
abstract: We investigated neutralizing antibodies to human papillomavirus type 16 (HPV-16) in serum and cervical washes from 84 women with normal cytology or cervical disease. Serum neutralizing antibodies were detected in 78 % of women infected at the cervix with HPV-16, compared with 35 % (P=0.002) of women infected with HPV-16-related types (alpha9 HPV types), 14 % (P<0.0001) of women infected with HPV-16 non-related types and none of HPV-uninfected women. A significant correlation between HPV-16 infection and serum HPV-16-neutralizing antibodies was observed (r(s)=0.97; P=0.032). Cervical neutralizing antibodies were detected in 38 % of women with HPV-16 infection and in 17 % of women infected with the HPV-16-related type HPV-31. Cervical neutralizing antibodies correlated with HPV-16 infection (r(s)=0.95; P=0.08), but not with cervical disease. Serum and cervical HPV-16 antibody responses were not affected significantly by human immunodeficiency virus type 1 infection. In conclusion, serum and cervical HPV-16-neutralizing antibodies were found to correlate with HPV-16 infection, but not with cervical disease.
authors.affiliation: National Health Laboratory Service, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa.|Institute of Infectious Disease and Molecular Medicine, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Zizipho Z A
authors.initials: ZZA
authors.lastname: Mbulawa
authors.affiliation: National Health Laboratory Service, Groote Schuur Hospital, Observatory, Cape Town 7925, South Africa.|Institute of Infectious Disease and Molecular Medicine, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Anna-Lise
authors.initials: AL
authors.lastname: Williamson
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Debbie
authors.initials: D
authors.lastname: Stewart
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Jo-Ann S
authors.initials: JS
authors.lastname: Passmore
authors.affiliation: Department of Obstetrics and Gynaecology, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Lynette
authors.initials: L
authors.lastname: Denny
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Bruce
authors.initials: B
authors.lastname: Allan
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town Medical School, Observatory, Cape Town 7925, South Africa.
authors.forename: Dianne J
authors.initials: DJ
authors.lastname: Marais
mesh_terms: D000328:Adult; D000349:Africa; D000914:Antibodies, Viral / Q000032:analysis*; D002584:Cervix Uteri / Q000276:immunology*; D005260:Female; D052162:Human papillomavirus 16 / Q000145:classification / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology*; D009500:Neutralization Tests; D030361:Papillomavirus Infections / Q000276:immunology*; D013019:South Africa; D002577:Uterine Cervical Diseases / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology; D002578:Uterine Cervical Dysplasia / Q000276:immunology
chemical_list: D000914:Antibodies, Viral