title: Efficiency of propolis extract against mitochondrial stress induced by antineoplasic agents (doxorubicin and vinblastin) in rats.
abstract: To assess the oxidative stress and mitochondrial dysfunction associated with disease, toxic process and aging, in vivo and in vitro preventive effect of propolis extract against mitochondrial oxidative stress induced by two anticancer drugs (doxorubicin and vinblastin) have been investigated in female wistar rat using liver and heart mitochondria. The results show that doxorubicin and vinblastin altered mitochondrial functions as observed by a decrease in respiratory control value, an activation of swelling and overproduction of superoxide anion. Myocardial tissue from doxorubicin treated rats showed a marked increase in malondialdehyde production, a depletion of reduced glutathione contents and an inhibition of catalase and superoxide dismutase activities. Similar results were also observed in liver tissue. Pretreatment of rats with propolis extract (100 mg/kg/day po) (10(-4) M ip) administered 4 days prior to doxorubicin (20 mg/kg) and/or vinblastin (2 mg/kg) injection, substantially reduced the peroxidative damage in myocardium and hepatic tissues and markedly restored the tissues catalase and SOD activities. The results strongly suggest that propolis extract protects heart and liver tissues from oxidative stress by protecting the mitochondria.
authors.affiliation: Laboratoire de Pharmacologie et Phytochimie, Faculté des Sciences, Université de Jijel. 18000 Jijel, Algérie.
authors.forename: Lamia
authors.initials: L
authors.lastname: Benguedouar
authors.forename: Hanane Nadia
authors.initials: HN
authors.lastname: Boussenane
authors.forename: Kebsa
authors.initials: K
authors.lastname: Wided
authors.forename: Mohamed
authors.initials: M
authors.lastname: Alyane
authors.forename: Hassiba
authors.initials: H
authors.lastname: Rouibah
authors.forename: Mesbah
authors.initials: M
authors.lastname: Lahouel
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D045424:Complex Mixtures / Q000494:pharmacology; D004195:Disease Models, Animal; D004317:Doxorubicin / Q000633:toxicity; D064420:Drug-Related Side Effects and Adverse Reactions / Q000188:drug therapy*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014747:Vinblastine / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D045424:Complex Mixtures; D014747:Vinblastine; D004317:Doxorubicin; D011429:Propolis; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase