A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry.
A simple and efficient method for C-terminal sequencing of proteins has long been pursued because it would provide substantial information for identifying the covalent structure, including post-translational modifications. However, there are still significant impediments to both direct sequencing from C termini of proteins and specific isolation of C-terminal peptides from proteins. We describe here a highly successful, de novo C-terminal sequencing method of proteins by employing succinimidyloxycarbonylmethyl tris (2,4,6-trimethoxyphenyl) phosphonium bromide and mass spectrometry.
Kuyama
Hiroki
H
Institute for Protein Research, Osaka University, Suita, Japan. kuyama@protein.osaka-u.ac.jp
Shima
Keisuke
K
Sonomura
Kazuhiro
K
Yamaguchi
Minoru
M
Ando
Eiji
E
Nishimura
Osamu
O
Tsunasawa
Susumu
S
D000818:Animals; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D002645:Chickens; D045304:Cytochromes c / Q000032:analysis*; D006736:Horses; D007202:Indicators and Reagents; D009113:Muramidase / Q000032:analysis*; D009943:Organophosphorus Compounds / Q000378:metabolism; D010047:Ovalbumin / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis*; D010449:Peptide Mapping; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D002364:Caseins; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds; D010446:Peptide Fragments; D011994:Recombinant Proteins; C405845:tris(trimethoxyphenyl)phosphonium acetyl; D010047:Ovalbumin; D045304:Cytochromes c; D009113:Muramidase