Ogataea nitratoaversa sp. nov., a methylotrophic yeast species from temperate forest habitats.
Three methanol-assimilating, nitrate-negative yeast strains representing a hitherto undescribed species, were isolated from leaf and rotten wood samples collected in temperate forests in Hungary. Analysis of the D1/D2 large subunit ribosomal RNA gene sequences placed the strains in the Ogataea clade. The three strains share identical D1/D2 and ITS sequences and significantly differ from the genetically most closely related species, Pichia pilisensis. Five substitutions in D1/D2 and more than 10% difference in the ITS regions were detected. A novel yeast species, Ogataea nitratoaversa, is proposed to accommodate these isolates. The type culture is NCAIM Y.01837(T) (CBS 10,796, NRRL Y-48,449). As the current description of the genus does not allow the inclusion of nitrate negative species, the emendation of the diagnosis of the genus Ogataea Yamada, Maeda and Mikata is proposed.
Péter
Gábor
G
National Collection of Agricultural and Industrial Microorganisms, Corvinus University of Budapest, Faculty of Food Sciences, Somlói út 14-16, 1118 Budapest, Hungary. gabor.peter@uni-corvinus.hu
Tornai-Lehoczki
Judit
J
Dlauchy
Dénes
D
D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006814:Hungary; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009566:Nitrates / Q000378:metabolism*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D013172:Spores, Fungal / Q000166:cytology; D014197:Trees / Q000382:microbiology*; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D009566:Nitrates; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D000432:Methanol