title: Cloning, expression pattern, chromosomal localization, and evolution analysis of Porcine gnaq, gna11, and gna14.
abstract: The Gq class is one subfamily of the G protein alpha subunits multigene family. It comprises four genes: Gnaq, Gna11, Gna14, and Gna15. In mice and humans, the alpha subunit is an essential component of G protein interaction with receptors and effectors. We report here the cloning and characterization of porcine Gnaq, Gna11, and Gna14. We have cloned the full-length coding sequences of porcine Gnaq, Gna11, and Gna14 (1,080, 1,080, and 1,068 bp, respectively) and then mapped them chromosomally to regions 1q21-27, 2q21-24, and 1q21-27 by radiation hybrid mapping. Tissue distribution analysis indicated that Gnaq and Gna11 were coexpressed in liver, heart, muscle, spleen, adipose tissue, brain, and uterus, but Gna14 mRNA was detected only in kidney and lung. The phylogenetic trees reveal that porcine Gnaq, Gna11, and Gna14 are evolutionarily closer to their human homologs. This is the first report of molecular cloning and characterization of porcine Gnaq, Gna11, and Gna14, which will be helpful for further understanding of the physiological role of Gq class genes in pigs.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Huaping
authors.initials: H
authors.lastname: Chen
authors.forename: Wenshu
authors.initials: W
authors.lastname: Yao
authors.forename: Dan
authors.initials: D
authors.lastname: Jin
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Lei
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Zaiqing
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D010802:Phylogeny; D021101:Radiation Hybrid Mapping; D013552:Swine / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D043802:GTP-Binding Protein alpha Subunits, Gq-G11