[Determination of oridonin and rosemarinic acid in Rabdosia rubescens by HPLC].
OBJECTIVE
To establish the method for determining the content of oridonin and rosemarinic acid in Rabdosia rubescens by HPLC.


METHODS
Using ODS-C18 column (150 mm x 4.6 mm, 5 microm), methanol-0.3% phosphoric acid (40:60), oridonin detection wavelength at 238 nm and rosemarinic acid at 329 nm.


RESULTS
The average recovery oridonin was 97. 1% and rosemarinic acid was 99.3%. Oridonin relative standard deviation (RSD) was 2.8% and rosemarinic acid was 2.9%.


CONCLUSIONS
This method can be used to determine the content of oridonin and rosemarinic acid in Rabdosia rubescens.
Wang
Kun
K
Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. wangkunsy@yahoo.com.cn
Zhang
Zhen-qiu
ZQ
D002109:Caffeic Acids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D045786:Diterpenes, Kaurane / Q000032:analysis* / Q000302:isolation & purification; D037602:Isodon / Q000737:chemistry*; D000432:Methanol; D010598:Pharmacognosy; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D000097123:Rosmarinic Acid
D002109:Caffeic Acids; D045786:Diterpenes, Kaurane; D000432:Methanol; D000097123:Rosmarinic Acid; C011959:oridonin