title: [Experimental study of baculovirus-mediated transfection of spiral ganglion cells in rats].
abstract: OBJECTIVE
  To study the feasibility and the characteristics of recombinant baculovirus as spiral ganglion cells (SGC) gene transfer vector.
  
  
  METHODS
  After the generation of baculovirus- green fluorescent protein( Bac-GFP) according to Bac-to-Bac baculovirus expression system, SGC were infected by Bac-GFP with different multiplicities of infection (MOI) and different concentrations of sodium butyrate. The transfection cell rate and mean fluorescence strength (MFS) were detected by fluorescence microscopy and flow cytometry. Toxicity effects of recombinant baculovirus vectors and sodium butyrate on SGC were determined by spectroscopic measurement of 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-diphenytetrazoliumromide (MTF).
  
  
  RESULTS
  Baculovirus was able to infect primary SGC cultures. The dose-response characteristics of Bac-GFP were determined on SGC, and the expression level could be up-regulated by sodium butyrate. Infection with Bac-GFP in the absence or presence of sodium butyrate (< or =10 mmol/L) was considered to be non-cytotoxic to primary SGC. GFP had been expressed in SGC at 6 h post-infection and the highest numbers of cells expressing GFP were observed at approximately 48 h post-infection.
  
  
  CONCLUSIONS
  Baculovirus is a novel and promising tool for gene transferring into the cochlear nervous system both for studies of the function of foreign genes and the development of gene therapy strategies.
authors.affiliation: Department of Otorhinolaryngology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Shi-li
authors.initials: SL
authors.lastname: Wang
authors.forename: Chang-ping
authors.initials: CP
authors.lastname: Cai
authors.forename: Biao
authors.initials: B
authors.lastname: Li
authors.forename: Yi-fan
authors.initials: YF
authors.lastname: Zhang
authors.forename: Sheng-ping
authors.initials: SP
authors.lastname: Hu
authors.forename: Xiang-long
authors.initials: XL
authors.lastname: Tian
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013136:Spiral Ganglion / Q000166:cytology; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins