Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: comparison among Levovist, YM454, and MRX-815H.
We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1MHz ultrasound at 0.2MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454>MRX-815H>Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.
Watanabe
Akihiko
A
Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
Otake
Remon
R
Nozaki
Tetsuo
T
Morii
Akihiro
A
Ogawa
Ryohei
R
Fujimoto
Shinichi
S
Nakamura
Shinobu
S
Fuse
Hideki
H
Kondo
Takashi
T
D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry* / Q000494:pharmacology; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D045423:Microbubbles; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D011471:Prostatic Neoplasms; D013010:Sonication; D014162:Transfection / Q000379:methods*
D003287:Contrast Media; D011134:Polysaccharides; C059891:SHU 508; D008156:Luciferases