title: Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue.
abstract: We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy.
authors.affiliation: Department of Chemistry, National Creative Research Initiative Center for Cell Dynamics, Department of Physics, Biomicrosystems Technology Program, Korea University, 1-Anamdong, Seoul, 136-701, Korea.
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Byeong Ha
authors.initials: BH
authors.lastname: Jeong
authors.forename: Ju-Yong
authors.initials: JY
authors.lastname: Hyon
authors.forename: Myoung Jin
authors.initials: MJ
authors.lastname: An
authors.forename: Mun Sik
authors.initials: MS
authors.lastname: Seo
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Kyoung J
authors.initials: KJ
authors.lastname: Lee
authors.forename: Chul Hoon
authors.initials: CH
authors.lastname: Kim
authors.forename: Taiha
authors.initials: T
authors.lastname: Joo
authors.forename: Seok-Cheol
authors.initials: SC
authors.lastname: Hong
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry*; D009924:Organ Culture Techniques; D017785:Photons; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats
chemical_list: C528316:6-((E)-3-oxo-1-dodecenyl)-2-(N-methyl-N-(carboxymethyl)amino)naphthalene; C528315:6-dodecanoyl-2-(N-methyl-N-(carboxymethyl)amino)naphthalene; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D009281:Naphthalenes