title: MicroRNA expression in preimplantation mouse embryos from Ped gene positive compared to Ped gene negative mice.
abstract: OBJECTIVE
  The mouse preimplantation embryo development (Ped) gene product, Qa-2, influences the rate of preimplantation embryonic development and overall reproductive success. Here we investigated the expression pattern of two microRNAs, miR-125a and miR-125b, known to be involved in development in lower organisms, in preimplantation embryos from the two-cell, four-cell, eight-cell, morula, and blastocyst stages of development from the congenic B6.K1 (Ped negative) and B6.K2 (Ped positive) strains of mice.
  
  
  METHODS
  B6.K1 and B6.K2 congenic mice differ only in the absence (B6.K1) or presence (B6.K2) of the genes encoding Qa-2 protein. We analyzed the expression of miR-125a and miR-125b in B6.K1 and B6.K2 preimplantation embryos by using real-time PCR.
  
  
  RESULTS
  We found no variability in miR-125b expression at any developmental stage in both strains. However, miR-125a expression increased during development in both strains and was ten times higher in Ped negative (B6.K1) embryos than in Ped positive (B6.K2) embryos by the blastocyst stage of development.
  
  
  CONCLUSIONS
  Our results show that the absence of the Ped gene profoundly affects the level of a miRNA (miR-125a) known to regulate early development. The implication is that miR-125a is likely involved in the regulation of timing of early development in mice.
authors.affiliation: Biology Department, Northeastern University, 134 Mugar Hall, 360 Huntington Avenue, Boston, MA, 02115, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Byrne
authors.forename: Carol M
authors.initials: CM
authors.lastname: Warner
mesh_terms: D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D011247:Pregnancy
chemical_list: D015395:Histocompatibility Antigens Class I; D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse; C032907:Q surface antigens