Macrophages are mediators of gastritis in acute Helicobacter pylori infection in C57BL/6 mice.
Helicobacter pylori is the etiological agent of human chronic gastritis, a condition seen as a precursor to the development of gastrointestinal ulcers or gastric cancer. This study utilized the murine model of chronic H. pylori infection to characterize the role of macrophages in the induction of specific immune responses and gastritis and in the control of the bacterial burden following H. pylori infection and vaccination. Drug-loaded liposomes were injected intravenously to deplete macrophages from C57BL/6 mice, and effective removal of CD11b+ cells from the spleens and stomachs of mice was confirmed by immunofluorescence microscopy. Transient elimination of macrophages from C57BL/6 mice during the early period of infection reduced the gastric pathology induced by H. pylori SS1 but did not affect the bacterial load in the stomach. These data suggest that macrophages are important to the severity of gastric inflammation during H. pylori infection.
Kaparakis
Maria
M
Department of Microbiology and Immunology, The University of Melbourne, Melbourne, VIC 3010, Australia. odilia@unimelb.edu.au.
Walduck
Anna K
AK
Price
Jason D
JD
Pedersen
John S
JS
van Rooijen
Nico
N
Pearse
Martin J
MJ
Wijburg
Odilia L C
OL
Strugnell
Richard A
RA
D000818:Animals; D039481:CD11b Antigen / Q000032:analysis; D015169:Colony Count, Microbial; D005260:Female; D005756:Gastritis / Q000276:immunology / Q000382:microbiology*; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification; D047589:Leukocyte Reduction Procedures; D008264:Macrophages / Q000737:chemistry / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D013154:Spleen / Q000166:cytology; D013270:Stomach / Q000166:cytology / Q000382:microbiology / Q000473:pathology
D039481:CD11b Antigen