title: Identification of new regional marker proteins to map mouse brain by 2-D difference gel electrophoresis screening.
abstract: To screen for new region-specific protein markers we compared the proteome maps of the primary visual and somatosensory areas V1 and S1 in mouse brain using 2-D difference gel electrophoresis (2-D DIGE). Twenty-three protein spots showed a statistically significant difference in expression level between V1 and S1, with 52% appearing more abundantly in V1. Twenty-six proteins were mass spectrometrically identified in 22 spots. To assess the validity of this list of potential areal markers generated by 2-D DIGE, the effective area-specific distribution profile of creatine kinase brain subtype (CKB), a protein with a clearly higher expression level in S1, was monitored with in situ hybridization. The mRNA expression profile of CKB displayed a clear area-specific distribution, which allowed demarcation of S1 and its topographical borders with neighboring neocortical areas. This proteomic study demonstrates the innovative application of 2-D DIGE and MS to select new regional markers for neuroscience research.
authors.affiliation: Laboratory of Neuroplasticity and Neuroproteomics, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Sandy
authors.initials: S
authors.lastname: Jacobs
authors.forename: Babs
authors.initials: B
authors.lastname: Van de Plas
authors.forename: Estel
authors.initials: E
authors.lastname: Van der Gucht
authors.forename: Stefan
authors.initials: S
authors.lastname: Clerens
authors.forename: Lieselotte
authors.initials: L
authors.lastname: Cnops
authors.forename: Gert
authors.initials: G
authors.lastname: Van den Bergh
authors.forename: Lutgarde
authors.initials: L
authors.lastname: Arckens
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins