title: The peripheral chromosome scaffold, a novel structural component of mitotic chromosomes.
abstract: Using an original high-salt extraction protocol, we observed a novel chromosome substructure, referred to as the peripheral chromosome scaffold. This chromosome domain contained the perichromosomal layer proteins pKi-67, B23/nucleophosmin and fibrillarin, but no DNA fragments (i.e., the loop domain bases were not associated with the peripheral scaffold). Modern models of chromosome organization do not predict the existence of a peripheral chromosome scaffold domain, and thus our observations have conceptual implications for understanding chromosome architecture.
authors.affiliation: Department of Electron Microscopy, AN Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russia. sheval_e@genebee.msu.su
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Sheval
authors.forename: Vladimir Y
authors.initials: VY
authors.lastname: Polyakov
mesh_terms: D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D002877:Chromosomes, Human / Q000737:chemistry / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000235:genetics*
chemical_list: D002868:Chromosomal Proteins, Non-Histone