title: Preparative scale Baeyer-Villiger biooxidation at high concentration using recombinant Escherichia coli and in situ substrate feeding and product removal process.
abstract: An efficient biocatalytic process based on the use of adsorbent resin (in situ substrate feeding and product removal) makes experiments at high substrate concentration possible by overcoming limitations due to substrate and product inhibition. This process was successfully applied to the preparative scale Baeyer-Villiger biooxidation of (-)-(1S,5R)-bicyclo[3.2.0]hept-2-en-6-one (25 g). Whole cells of recombinant E. coli (1 liter) overexpressing cyclohexanone monooxygenase were used as a biocatalyst and the substrate was preloaded onto the adsorbent resin. The corresponding lactone was obtained in 75-80% yield. Time for cell growth and biotransformation is about 24 h each and oxygen supply can be improved by using a tailor-made bubble column.
authors.affiliation: Biosciences CNRS 3005, Aix-Marseille Université, Avenue Escadre Normandie-Niemen, 13397 Marseille, France.
authors.forename: Iris
authors.initials: I
authors.lastname: Hilker
authors.forename: Maria C
authors.initials: MC
authors.lastname: Gutiérrez
authors.forename: Roland
authors.initials: R
authors.lastname: Furstoss
authors.forename: John
authors.initials: J
authors.lastname: Ward
authors.forename: Roland
authors.initials: R
authors.lastname: Wohlgemuth
authors.forename: Véronique
authors.initials: V
authors.lastname: Alphand
mesh_terms: D019149:Bioreactors; D001711:Biotransformation; D002384:Catalysis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007783:Lactones / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D011995:Recombination, Genetic; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D007783:Lactones; D012117:Resins, Synthetic