A new miracidia hatching device for diagnosing schistosomiasis.
It is still imperative to develop a parasitological technique highly sensitive for diagnosing schistosomiasis in epidemiological and individual surveys. A simple and cheap hatching device with a collecting container was manufactured and tested under experimental conditions. Twelve Kato-Katz slides were performed as golden standard for comparison. Quantitative results can be carried out by counting miracidia in a plate and parasite load can be calculated (miracidia/gram of feces). Statistically significant values were higher in the hatching test. More sensitive results, with statistical significance, were achieved using 1.5 g of feces (which corresponds to 36 Kato-Katz slides) than by using the Kato-Katz method. Advantages of this technique and its limitations are presented.
Jurberg
Arnon D
AD
Laboratório de Patologia, Instituto Oswaldo Cruz-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil .
Oliveira
Aureo A de
AA
Lenzi
Henrique L
HL
Coelho
Paulo Marcos Z
PM
D000818:Animals; D006224:Cricetinae; D005243:Feces / Q000469:parasitology*; D010270:Parasite Egg Count / Q000295:instrumentation* / Q000379:methods; D012550:Schistosoma mansoni / Q000302:isolation & purification*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012680:Sensitivity and Specificity