Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes.
A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.
Takagi
Mutsumi
M
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, 060-8628, Japan. takagi-m@eng.hokudai.ac.jp
Kitabayashi
Takayuki
T
Koizumi
Satoru
S
Hirose
Haruka
H
Kondo
Shin-Ichi
S
Fujiwara
Masashi
M
Ueno
Kosei
K
Hiroaki
Misawa
M
Hosokawa
Yoichiroh
Y
Masuhara
Hiroshi
H
Wakitani
Shigeyuki
S
D053674:Aggrecans / Q000096:biosynthesis*; D015703:Antigens, CD / Q000096:biosynthesis; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology*; D048430:Cell Shape / Q000502:physiology*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D005326:Fetal Proteins / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D018800:Thy-1 Antigens / Q000096:biosynthesis; D020410:Activated-Leukocyte Cell Adhesion Molecule
D053674:Aggrecans; D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D012333:RNA, Messenger; D018800:Thy-1 Antigens; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule