title: Transcription factor GATA-3 regulates the transcriptional activity of dopamine beta-hydroxylase by interacting with Sp1 and AP4.
abstract: GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine beta-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein-protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.
authors.affiliation: Molecular Neurobiology Laboratory, MRC215, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, MA, 02478, USA.
authors.forename: Seok Jong
authors.initials: SJ
authors.lastname: Hong
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Sunghoi
authors.initials: S
authors.lastname: Hong
authors.forename: Youngbuhm
authors.initials: Y
authors.lastname: Huh
authors.forename: Han
authors.initials: H
authors.lastname: Chae
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004299:Dopamine beta-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D009474:Neurons / Q000166:cytology / Q000502:physiology; D011401:Promoter Regions, Genetic; D011784:Quail; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D050990:GATA3 Transcription Factor; D011993:Recombinant Fusion Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D004299:Dopamine beta-Hydroxylase