title: Proteomic identification of differentially expressed genes in mouse neural stem cells and neurons differentiated from embryonic stem cells in vitro.
abstract: Embryonic stem (ES) cells are pluripotent stem cells and give rise to a variety of differentiated cell types including neurons. To study a molecular basis for differentiation from ES cells to neural cells, we searched for proteins involved in mouse neurogenesis from ES cells to neural stem (NS) cells and neurons by two-dimensional gel electrophoresis (2-DE) and peptide mass fingerprinting, using highly homogeneous cells differentiated from ES cells in vitro. We newly identified seven proteins with increased expression and one protein with decreased expression from ES cells to NS cells, and eight proteins with decreased expression from NS cells to neurons. Western blot analysis confirmed that a tumor-specific transplantation antigen, HS90B, decreased, and an extracellular matrix and membrane glycoprotein (such as laminin)-binding protein, galectin 1 (LEG1), increased in NS cells, and LEG1 and a cell adhesion receptor, laminin receptor (RSSA), decreased in neurons. The results of RT-PCR showed that mRNA of LEG1 was also up-regulated in NS cells and down-regulated in neurons, implying an important role of LEG1 in regulating the differentiation. The differentially expressed proteins identified here provide insight into the molecular basis of neurogenesis from ES cells to NS cells and neurons.
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, Inage-Ku, Chiba, Japan. akama@faculty.chiba-u.jp
authors.forename: Kuniko
authors.initials: K
authors.lastname: Akama
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Tatsuno
authors.forename: Masahiro
authors.initials: M
authors.lastname: Otsu
authors.forename: Tomoe
authors.initials: T
authors.lastname: Horikoshi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakayama
authors.forename: Megumi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Tosifusa
authors.initials: T
authors.lastname: Toda
authors.forename: Nobuo
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger