Influence of a quantitative trait locus on mouse chromosome 19 to the light-adapted electroretinogram.
OBJECTIVE
Both implicit time and amplitude of the cone-mediated electroretinographic (ERG) b-wave differ significantly between the C57BL/6JOlaHsd and 129S2/SvHsd inbred mouse strains. The purpose of this work was to undertake a quantitative genetics study to localize the gene or genes involved.


METHODS
Implicit time and amplitude of the a- and b-waves of the single-flash and flicker cone-mediated ERG were recorded as the quantitative traits in reciprocal backcrossed populations. A genome-wide scan was performed with 106 polymorphic markers. Map Manager (release QTXb20) was used to analyze the data and make phenotype-genotype correlations.


RESULTS
A quantitative trait locus (QTL) of major effect in controlling variation in both implicit time and amplitude of the cone-mediated ERG localized to the middle of chromosome 19.


CONCLUSIONS
Mapping of a QTL influencing both implicit time and b-wave amplitude of the light-adapted ERG represents an initial step toward identifying the gene(s) responsible for this phenotypic variation.
Reynolds
Alison L
AL
Smurfit Institute of Genetics, Trinity College, Dublin, Ireland. reynolds.alison@gmail.com
Danciger
Michael
M
Farrar
G Jane
GJ
Humphries
Peter
P
Kenna
Paul F
PF
D000064:Acclimatization; D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004596:Electroretinography; D005838:Genotype; D008027:Light; D051379:Mice; D008815:Mice, Inbred Strains; D040641:Quantitative Trait Loci; D013045:Species Specificity; D013623:Tail
D004247:DNA