[Fast and simultaneous determination of four index components in Qingwei Huanglian tablets by RP-HPLC].
OBJECTIVE
To establish a RP-HPLC method for fast and simultaneous determination of four index contents, which are Genpioside, Baicalin, Berberine Hydrochloride and Ammonium Glyeyrrhizinate, in Qingwei Huanglian tablets.


METHODS
A separation was well achieved by gradient elution on a Hypersil C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phases of acetontrile -0.5% triethylamine water-solution (pH 3.0) at a flow rate of 1.0 mL x min(-1), detection wavelength of 230 nm, and room temperature.


RESULTS
The calibration curve were linear in the ranges of 4.82-77.2, 5.80-92.8, 1.63-26.1 and 6.40-102 microg x mL(-1) (r = 0.9997-0.9999) for genpioside, baicalin, berberine hydrochloride and ammonium glyeyrrhizinate, respectively. The average recoveries of four index components were not less than 98.0%.


CONCLUSIONS
The method is convenient, rapid and accurate. It is suitable for the quality control of Qingwei Huanglian tablets.
Li
Yan-Rong
YR
School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China.
Jiang
Ye
Y
Hao
Fu
F
Ding
Xiang-Yu
XY
D001599:Berberine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D039323:Coptis / Q000737:chemistry; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D005419:Flavonoids / Q000032:analysis*; D032062:Gardenia / Q000737:chemistry; D006035:Glycyrrhiza / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry; D011786:Quality Control; D031364:Scutellaria baicalensis / Q000737:chemistry; D013607:Tablets
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D013607:Tablets; D001599:Berberine; C007835:geniposide; C038044:baicalin; D019695:Glycyrrhizic Acid