Improved performance of enzyme-linked immunosorbent assays and the effect of human immunodeficiency virus coinfection on the serologic detection of herpes simplex virus type 2 in Rakai, Uganda.
Ugandan subjects (820) were tested by Focus HerpeSelect enzyme-linked immunosorbent assay (ELISA), Kalon herpes simplex virus type 2 ELISA, and BioKit rapid test, and the results were compared to those of Western blotting. Higher-than-standard-index cutoff values gave optimal sensitivity and specificity. Kalon ELISA was the optimal assay when an index value of 1.5 was used (sensitivity, 91.7%; specificity, 92.4%).
Gamiel
Jordyn L
JL
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Tobian
Aaron A R
AA
Laeyendecker
Oliver B
OB
Reynolds
Steven J
SJ
Morrow
Rhoda Ashley
RA
Serwadda
David
D
Gray
Ronald H
RH
Quinn
Thomas C
TC
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000150:complications; D006558:Herpes Genitalis / Q000175:diagnosis* / Q000453:epidemiology; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D008297:Male; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014454:Uganda / Q000453:epidemiology
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic