Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules.
A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K(d) = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.
Constantin
Tudor P
TP
Department of Chemistry and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213-3890, USA.
Silva
Gloria L
GL
Robertson
Kelly L
KL
Hamilton
Tamara P
TP
Fague
Kaitlin
K
Waggoner
Alan S
AS
Armitage
Bruce A
BA
D002232:Carbocyanines / Q000138:chemical synthesis* / Q000737:chemistry; D005461:Fluorine / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D002232:Carbocyanines; D005461:Fluorine; D012313:RNA