The influence of the adiponectin gene on adiponectin concentrations and parameters of metabolic syndrome in non-diabetic Korean women.
BACKGROUND
Concentrations of adiponectin, the protein product of the adipocyte C1q and collagen-domain-containing (ADIPOQ) gene are associated with type 2 diabetes and coronary artery disease. We investigate the association of single-nucleotide polymorphisms (SNPs) in the ADIPOQ gene with adiponectin concentrations, and to parameters of metabolic syndrome.


METHODS
867 unrelated, non-diabetic Korean women, 20 to 69 y, were genotyped for 8 SNPs in the ADIPOQ gene (-11391G>A, -11377C>G, H241P, Y111H, G90S, R221S, 45T>G, 276G>T). Adiponectin, a homeostasis model assessment of insulin resistance (HOMA-IR), and metabolic parameters were measured.


RESULTS
Carriers of genotype T/T at position 276 had significantly higher adiponectin concentrations than G/G carriers (P=0.005). Homozygous carriers of the TG haplotype (i.e., individuals who were T/T at 45 and G/G at 276) and heterozygous carriers of the TG haplotype (TG/X) had lower adiponectin concentrations than non-TG carriers (P<0.001). Significant associations between SNP at 276 and serum concentrations of triglyceride (P=0.013), insulin (P=0.013) and HOMA-IR (P=0.012) were found. The 45-276 haplotypes had associations identical to the 276G>T SNP. In subgroup analysis, subjects carrying the TG haplotype had significantly lower adiponectin concentrations than non-TG carriers in both normal weight (P<0.001) and overweight-obese (P=0.009) subgroups. The association of the TG haplotype with increasing insulin concentrations was significant among overweight-obese subjects (P=0.004), but was not significant among normal weight subjects. A similar association was found between the 45-276 haplotype and HOMA-IR.


CONCLUSIONS
There is a strong association of the adiponectin SNP276 genotypes and the adiponectin 45-276 haplotypes with circulating adiponectin concentrations in non-diabetic Korean women. In addition, this haplotype is associated with increased insulin concentrations and insulin resistance index only in overweight-obese individuals.
Jang
Yangsoo
Y
Cardiovascular Genome Center, Cardiovascular Hospital, Severance Hospital, Seoul, Korea.
Chae
Jey Sook
JS
Koh
Soo Jeong
SJ
Hyun
Yae Jung
YJ
Kim
Ji Young
JY
Jeong
Yeo Jin
YJ
Park
Sungha
S
Ahn
Chul-Min
CM
Lee
Jong Ho
JH
D052242:Adiponectin / Q000097:blood* / Q000235:genetics*; D000328:Adult; D000368:Aged; D005260:Female; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007328:Insulin / Q000097:blood; D007723:Korea / Q000453:epidemiology; D024821:Metabolic Syndrome / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity; D020641:Polymorphism, Single Nucleotide; D014280:Triglycerides / Q000097:blood
D052242:Adiponectin; D007328:Insulin; D014280:Triglycerides