title: [How translation termination factor eRF1 Euplotes does not recognise UGA stop codon].
abstract: In universal-code eukaryotes, a single class-1 translation termination factor eRF1 decodes all three stop codons, UAA, UAG, and UGA. In some ciliates with variant genetic codes one or two stop codons are used to encode amino acid(s) and are not recognized by eRF1. In Stylonychia, UAG and UAA codons are reassigned as glutamine codons, and in Euplotes, UGA is reassigned as cysteine codon. In omnipotent eRF1s, stop codon recognition is associated with the N-terminal domain of eRF1. Because variant-code ciliates most likely evolved from universal code ancestor(s), structural features should exist in ciliate eRF1s that restrict their stop codon recognition. To find out amino acid residues which confer UAR-only specificity to Euplotes aediculatus eRF1, eRFI chimeras were constructed by swapping eRF1 E. aediculatus N-terminal domain sequences with the matching ones from the human protein. In these chimeras the MC-domain was from human eRF1. Functional analysis of these chimeric eRFI highlighted the crucial role of the two regions (positions 38-50 and 123-145) in the N-terminal domain of E. aediculatus eRF1 that restrict E. aediculatus eRF1 specificity toward UAR codons. Possibly, restriction of eRF1 specificity to UAR codons might have been an early event occurring in independent instances in ciliate evolutionary history, possibly facilitating the reassignment of UGA to sense codons.
authors.forename: S A
authors.initials: SA
authors.lastname: Lekomtsev
authors.forename: P M
authors.initials: PM
authors.lastname: Kolosov
authors.forename: L Iu
authors.initials: LIu
authors.lastname: Frolova
authors.forename: L
authors.initials: L
authors.lastname: Bidou
authors.forename: J-P
authors.initials: JP
authors.lastname: Rousset
authors.forename: L L
authors.initials: LL
authors.lastname: Kiselev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D018388:Codon, Terminator; D016812:Euplotes / Q000235:genetics* / Q000502:physiology; D005815:Genetic Code; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018388:Codon, Terminator; C489002:ETF1 protein, human; D010454:Peptide Termination Factors; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins