HPLC analysis of in vivo metabolites of 4-nitrobenzoic acid [(5-nitro-2-thiopheneyl)methylene]hydrazide in rats.
The in vivo metabolism of 4-nitrobenzoic acid [(5-nitro-2-thiopheneyl)methylene]hydrazide (substrate), a model that represents hydrazide hydrazone compounds, was investigated in the rat. The metabolites were monitored in rat plasma at certain time intervals. The substrate was dissolved in dimethylsulfoxide (DMSO)/water (1:4) and administered intraperitoneally at dose of 100 mg/kg and 500 mg/kg. Blood samples were collected at 30 min, then at 1,2, 4, 8, 12 and 24 h post-administration. The chromatographic separation of the substrate and its metabolites was performed on aNovapak C18 (Phenomenex) (150 mm x 4.6 mm i.d., 5-microm particle size) using a mobile phase consisting of phosphate buffer: acetonitrile (90:10, v/v) with a linear gradient system. From the biotransformation of this compound, 4-nitrobenzoic acid (M3) was identified together with the substrate, as evidenced by high pressure liquid chromatography (HPLC)-UV/diode array detection (DAD).
Koçyiğit-Kaymakçioğlu
Bedia
B
Department of Pharmaceutical Chemistry, Marmara University, Haydarpaşa Istanbul, Turkey.
Unsalan
Seda
S
Küçükgüzel
S Güniz
SG
Sener
Göksel
G
Rollas
Sevim
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006835:Hydrazones / Q000097:blood*; D008297:Male; D009579:Nitrobenzoates / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D013876:Thiophenes / Q000097:blood*
C529052:4-nitrobenzoic acid ((5-nitro-2-thiopheneyl)methylene)hydrazide; D006835:Hydrazones; D009579:Nitrobenzoates; D013876:Thiophenes; C008629:4-nitrobenzoic acid