title: The effect of weight loss on inflammation in obese women with polycystic ovary syndrome.
abstract: BACKGROUND
  The aim of the present study was to evaluate the effect of modest weight reduction on serum concentrations of tumour necrosis factor alpha (TNF-alpha), TNF soluble receptors (sTNFRs) and interleukin-6 (IL-6) in obese women with polycystic ovary syndrome (PCOS).
  
  
  METHODS
  The study group consisted of 15 obese women with PCOS (mean age 28.5 +/- 7.7 years). Serum concentrations of TNF-alpha, sTNFRs and IL-6, insulin, FSH, LH, DHEAS, androstendione, total and free testosterone, cortisol, 17OH-progesterone, oestradiol and sex hormone binding globulin (SHBG), glucose, total cholesterol, HDL cholesterol and triglycerides were measured before treatment and after 10% weight loss. All patients were advised to follow a 1000-1200 kcal diet with a limited intake of simple carbohydrate and animal fats and to exercise regularly (30 min, 3 times a week). Body composition was measured by bioimpedance. Serum concentrations of TNF-alpha, sTNFRs and IL-6 were determined by enzyme linked immunosorbent assay (ELISA). Plasma insulin, FSH, LH, DHEAS, androstendione, total and free testosterone, cortisol, 17OH-progesterone, oestradiol and SHBG were measured by a commercial RIA. Blood glucose, total cholesterol, HDL cholesterol and triglycerides were measured by an enzymatic procedure.
  
  
  RESULTS
  We observed no differences in serum concentrations of TNF-alpha, sTNFRs or IL-6 after treatment.
  
  
  CONCLUSIONS
  It seems that more than a modest weight reduction is necessary to obtain a decrease in serum concentrations of proinflammatory cytokines and an improvement in ovarian function in obese women with polycystic ovary syndrome.
authors.affiliation: Department of Pathophysiology, Medical University of Silesia, Katowice, Poland. magols@esculap.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Olszanecka-Glinianowicz
authors.forename: Barbara
authors.initials: B
authors.lastname: Zahorska-Markiewicz
authors.forename: Piotr
authors.initials: P
authors.lastname: Kocełak
authors.forename: Joanna
authors.initials: J
authors.lastname: Janowska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Semik-Grabarczyk
mesh_terms: D000328:Adult; D000735:Androstenedione / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood; D004038:Diet, Reducing; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007249:Inflammation / Q000097:blood* / Q000150:complications; D007328:Insulin / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000150:complications / Q000178:diet therapy; D011044:Poland; D011085:Polycystic Ovary Syndrome / Q000097:blood* / Q000150:complications / Q000178:diet therapy*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000097:blood; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000097:blood; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism; D018709:Statistics, Nonparametric; D013739:Testosterone / Q000097:blood; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D015431:Weight Loss
chemical_list: D001786:Blood Glucose; D008076:Cholesterol, HDL; D007328:Insulin; D015850:Interleukin-6; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D012738:Sex Hormone-Binding Globulin; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D002784:Cholesterol; D006854:Hydrocortisone