Identification and characterization of Lactic Acid Bacteria isolated from Tibetan Qula cheese.
Fourteen strains of Lactic Acid Bacteria (LAB) isolated from Qula, a Tibetan traditional yak cheese, were divided into four groups (A-D) according to morphological and biochemical characteristics. On the basis of the 16S rRNA gene sequence analysis, group A and group B strains were placed in the cluster making up the genus Leuconostoc, which together with Leuconostoc mesenteroides and Leuconostoc pseudomesenteroides, formed a distinct cluster. The group C strain was clearly identified as Enterococcus faecium by forming a very well defined cluster with this species. The group D strains were placed in the lactobacilli cluster with Lactobacillus plantarum and Lactobacillus pentosus being the closely related species. On the basis of DNA-DNA hybridization, strains in the groups A, B, C and D were identified as Leuconostoc mesenteroides subsp. dextranicum, Leuconostoc pseudomesenteroides, Enterococcus faecium and Lactobacillus plantarum, respectively. Leuconostoc mesenteroides subsp. dextranicum was the dominate member of the population.
Duan
Yuheng
Y
Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Henan, China.
Tan
Zhongfang
Z
Wang
Yanping
Y
Li
Zongwei
Z
Li
Zongyi
Z
Qin
Guangyong
G
Huo
Yuping
Y
Cai
Yimin
Y
D001482:Base Composition; D002611:Cheese / Q000382:microbiology*; D016984:Enterococcus faecium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D005285:Fermentation; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007934:Leuconostoc / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018609:Tibet
D012336:RNA, Ribosomal, 16S