Effects of tyrosine kinase and phosphatase inhibitors on microtubules in Arabidopsis root cells.
To investigate the role of tyrosine phosphorylation/dephosphorylation processes in plant cells the morphology of Arabidopsis thaliana primary roots and the organization of cortical microtubules (MTs) were studied after inhibition of protein tyrosine kinases (PTKs) and tyrosine phosphatases (PTPs). It was found that all tested types of PTKs inhibitors (herbimycin A, genistein and tyrphostin AG 18) altered root hair growth and development, probably as a result of their significant influences on MTs organization in root hairs. The treatment also led to MTs reorientation and disruption in epidermis and cortex cells of both elongation and differentiation zones of primary roots. Enhanced tyrosine phosphorylation after treatment with a PTPs inhibitor (sodium orthovanadate) resulted in intense induction of root hair development and growth and caused a significant shortening of the elongation zone. It also led to changes of MTs orientation from transverse to longitudinal in epidermis and cortex cells of the elongation and differentiation zones of the root. From the data obtained we can suppose that tyrosine phosphorylation can be involved in the dynamics and organization of MTs in different types of plant cells.
Yemets
Alla
A
Institute of Cell Biology and Genetic Engineering, Kiev, Ukraine. alyemets@univ.kiev.ua
Sheremet
Yarina
Y
Vissenberg
Kris
K
Van Orden
Jürgen
J
Verbelen
Jean-Pierre
JP
Blume
Yaroslav B
YB
D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D016227:Benzoquinones / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D018517:Plant Roots / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D017828:Rifabutin / Q000031:analogs & derivatives; D020032:Tyrphostins / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D016227:Benzoquinones; D004791:Enzyme Inhibitors; D047029:Lactams, Macrocyclic; D047428:Protein Kinase Inhibitors; D020032:Tyrphostins; D017828:Rifabutin; D014638:Vanadates; C020754:herbimycin; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D017027:Protein Tyrosine Phosphatases; C060641:tyrphostin A23