title: Kinetic profiles of 18 systemic pro- and anti-inflammatory mediators during and following exercise in children.
abstract: While acute changes in systemic pro-/antiinflammatory cytokines occur with exercise, individual kinetics during and following exercise remain unclear; particularly, information is scarce regarding children. This study investigated the exercise-induced kinetic profiles of major pro-/anti-inflammatory mediators in 21 healthy children (13.9 +/- 0.8 yr, 7 M/14 F). Exercise was 30 min of intermittent cycling at approximately 80% VO2max. Multiple blood samples were drawn at baseline, during, and following exercise for cytokines assay. IL-1alpha, IL-6, IL-17, IL-8, IP-10, MIP-1alpha, and MIP-1beta initially decreased (nadir: 14-19 min into exercise) and subsequently exceeded baseline levels (peaks: 20-24 min into exercise). TNF-alpha, IL-12p70, IL-1RA, IL-4, EGF, TGF-alpha, GM-CSF, Eotaxin, and MCP-1 were moderately and persistently decreased throughout. VEGF was unchanged; sCD40L was elevated during exercise and recovery. Our results indicate that key immunomodulators display non-linear, biphasic kinetic profiles in response to exercise, suggesting that detection of exercise-induced changes over baseline may depend on exercise duration and sampling timing.
authors.affiliation: Department of Pharmacology, School of Medicine, University of California, Irvine, CA, USA. jsrosa@uci.edu
authors.forename: Jaime S
authors.initials: JS
authors.lastname: Rosa
authors.forename: Stacy R
authors.initials: SR
authors.lastname: Oliver
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Flores
authors.forename: Scott C
authors.initials: SC
authors.lastname: Graf
authors.forename: Andria M
authors.initials: AM
authors.lastname: Pontello
authors.forename: Milagros
authors.initials: M
authors.lastname: Lbardolaza
authors.forename: Frank P
authors.initials: FP
authors.lastname: Zaldivar
authors.forename: Pietro R
authors.initials: PR
authors.lastname: Galassetti
mesh_terms: D000293:Adolescent; D000893:Anti-Inflammatory Agents / Q000097:blood*; D023201:CD40 Ligand / Q000097:blood; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test / Q000379:methods; D005260:Female; D006801:Humans; D007118:Immunoassay; D007155:Immunologic Factors / Q000097:blood; D018836:Inflammation Mediators / Q000097:blood*; D053582:Interleukin-1alpha / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007700:Kinetics; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D010809:Physical Fitness / Q000502:physiology; D012741:Sexual Maturation / Q000502:physiology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D007155:Immunologic Factors; D018836:Inflammation Mediators; D053582:Interleukin-1alpha; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D023201:CD40 Ligand