An optical rotatory detector for high-performance liquid chromatography using polarization modulation.
A sensitive and variable-wavelength optical rotatory (OR) detector for high-performance liquid chromatography is presented. This design is entirely different from that of conventional OR detectors consisting of a crossed polarizer pair. By placing a polarizing prism and a retardation plate into a commercial circular dichroism (CD) detector, the OR signal was obtained. The Mueller matrix approach was used to prove the principle of the OR signal appearance. Sugars and 4-androstene-3,17-dione were chosen as test compounds. The limit of detection was below 0.5 microg of injected sucrose at 260 nm, which was superior to that obtained with a conventional OR detector. For 4-androstene-3,17-dione, which is CD active, and shows a large anomalous OR dispersion curve, our detector gave a large OR signal with approximately half the intensity of the CD signal at 340 nm.
Yamamoto
Atsushi
A
College of Bioscience and Biotechnology, Chubu University, Matsumoto, Kasugai, Aichi, Japan. akmiy@isc.chubu.ac.jp
Kawai
Mio
M
Sakamoto
Mitsunori
M
Kodama
Shuji
S
Hayakawa
Kazuichi
K
D000465:Algorithms; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D002942:Circular Dichroism / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D009904:Optical Rotation; D009905:Optical Rotatory Dispersion / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors
D002241:Carbohydrates