title: Generation of transparency and cellular organization in lens explants.
abstract: The lens grows via the proliferation and differentiation of lens epithelial cells into lens fibres. This differentiation process, thought to be controlled by factors present in the vitreous fluid, generates tightly-packed, parallel-aligned fibre cells that confer transparency to the lens. Using lens epithelial-cell explants we examined how explant orientation and growth factor treatment can affect cellular arrangement and explant transparency. Fibre cell differentiation was induced in lens explants by culturing cells with fibroblast growth factor (FGF) or bovine vitreous. Cell shape and arrangement was investigated using confocal microscopy, electron microscopy, immunofluorescence and in situ hybridization. Explant transparency was measured using light microscopy. Confocal microscopy demonstrated that explant orientation determined cellular arrangement, irrespective of the differentiation stimuli used. In explants where epithelial cells were confined between their normal basement membrane (the lens capsule) and the base of the culture dish, the cells became elongated, thin and parallel-aligned. In contrast, in explants cultured with cells directly exposed to the culture media the cells appeared to be shorter, globular and haphazardly arranged. FGF initiated the differentiation of most lens epithelial cells; however, abnormal cellular morphologies developed with subsequent culture of the cells. As a result, the transparency of these explants decreased with prolonged culture. Interestingly, explants cultured with vitreous (i) did not develop abnormal cellular morphologies, (ii) contained two distinct cell types (retained epithelial cells and newly differentiated fibre cells) and (iii) remained transparent throughout the lengthy culture period. In summary, we have developed a culture system that generates a transparent tissue with a cellular arrangement resembling that of the lens in vivo. We have shown that while FGF and vitreous initiate differentiation within this system, better maintenance of fibre cell integrity, more appropriate regulation of molecular events, and better maintenance of explant transparency was achieved in the presence of vitreous. This system offers an opportunity to further investigate the process of lens fibre cell differentiation as well as a means of better identifying the factors that contribute to the development of tissue transparency in vitro.
authors.affiliation: Save Sight Institute and Department of Clinical Ophthalmology & Eye Health, The University of Sydney, Sydney, NSW 2006, Australia. moconnor@bccrc.ca
authors.forename: Michael D
authors.initials: MD
authors.lastname: O'Connor
authors.forename: Elizabeth D
authors.initials: ED
authors.lastname: Wederell
authors.forename: Robert
authors.initials: R
authors.lastname: de Iongh
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Lovicu
authors.forename: John W
authors.initials: JW
authors.lastname: McAvoy
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D017403:In Situ Hybridization; D007903:Lens Capsule, Crystalline / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D018613:Microscopy, Confocal; D008938:Mitosis / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012542:Scattering, Radiation; D046509:Tissue Culture Techniques; D014822:Vitreous Body / Q000502:physiology
chemical_list: D016222:Fibroblast Growth Factor 2