The cognitive enhancer SGS742 does not involve major known signaling cascades in OF1 mice.
In a previous study we have shown that SGS742, a cognitive enhancer acting by GABA(B) receptor antagonism improved spatial learning in OF1 mice. The aim of the study was therefore to screen representatives of several signaling cascades known to be involved in memory formation at the protein level. NaCl-, SGS742- treatment and yoked controls for NaCl and SGS742 were studied. Six hours following testing OF1 mice in the Morris water maze, hippocampal signaling proteins calmodulin kinase II (CaMKII) (phosphorylated (P) and non-phosphorylated (NP) form), CREB (P, NP), ERK (P, NP), BDNF, Egr-1, Trk-B and neuro-cytoskeleton elements drebrin and PSD-95 were determined by immunoblotting. Protein patterns were comparable between the control and SGS742 treated group revealing that these proteins may not be involved in the mechanisms underlying cognitive enhancement by SGS742 and this should be taken into account for interpretation and design of previous and future related neurochemical studies.
Sunyer
Berta
B
Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18-20, Vienna 1090, Austria.
Shim
Ki-Shuk
KS
Höger
Harald
H
Lubec
Gert
G
D000818:Animals; D015153:Blotting, Western; D003463:Cues; D058787:GABA-A Receptor Antagonists; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013028:Space Perception / Q000187:drug effects
D058787:GABA-A Receptor Antagonists; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D009943:Organophosphorus Compounds; C079148:(3-aminopropyl)(n-butyl)phosphinic acid