[Detection the coinfection of Hantavirus and Orientia tsutsugamushi in primary cultured mite cells].
OBJECTIVE
To investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).


METHODS
Collecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear. In situ RT-PCR and PCR were used to detect and locate HV RNA and Ot DNA in the primary cultured cells.


RESULTS
Positive signals of HV RNA and Ot DNA distributed mostly in epithelial cells of digestive system and ovary cells of larva and nymph. The positive rate increased by the generation of passages.


CONCLUSIONS
Coinfection of HV and Ot did exist in wild Leptotrombidium scutellare.
Deng
Xiao-zhao
XZ
Institute of Military Medicine, Nanjing Command, Nanjing 210002, China.
Xu
Ke
K
Jiang
Chun-mei
CM
Wang
Zhong-can
ZC
Kong
Jing
J
Diao
Zhen-yu
ZY
Qian
Jun-ying
JY
Zhang
Yun
Y
Cao
Guang-wen
GW
D000818:Animals; D002478:Cells, Cultured; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006476:Orthohantavirus / Q000302:isolation & purification*; D051379:Mice; D008925:Mites / Q000382:microbiology* / Q000821:virology*; D012285:Orientia tsutsugamushi / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004269:DNA, Bacterial; D012367:RNA, Viral