title: [Activation related genes of memory CD(4)(+) T cells in asthma patients].
abstract: OBJECTIVE
  To screen and identify the genes of activated memory CD(4)(+) T cells in asthma.
  
  
  METHODS
  Differential display polymerase chain reaction (DDPCR) was utilized to identify genes of memory CD(4)(+) T cells after activation from asthmatic patients and normal individuals, and the mRNA levels of the differentially expressed genes were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Nineteen differentially expressed cDNA fragments were isolated. The homology analysis indicated that two fragments possessed a high degree of homology with interleukin-7 (IL-7) mRNA and MM-1 mRNA. RT-PCR confirmed that IL-7 mRNA and MM-1 mRNA levels were increased in memory CD(4)(+) T cells after activation in asthmatic patients (0.390 +/- 0.029, 0.629 +/- 0.047, F = 983, 1264 respectively, all P < 0.05).
  
  
  CONCLUSIONS
  The up-regulated expression of IL-7 and MM-1 genes in memory CD(4)(+) T cells after activation may be a molecular mechanism that differentiates the response to allergens of asthma patients from normal individuals.
authors.affiliation: Department of Respiratory Medicine, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Pi
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Guo
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: Xu
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Ni
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Xu
mesh_terms: D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D015851:Interleukin-7 / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D015851:Interleukin-7; C115636:PFDN5 protein, human; D012333:RNA, Messenger; D012097:Repressor Proteins