Complete genomic characterization of an intertypic Sabin 3/Sabin 2 capsid recombinant.
The genetic properties of strain K/2002, isolated from fecal samples of a 7-month-old child who had received his first oral poliovirus vaccine (OPV) dose at the age of 3 months, are described. Preliminary sequencing characterization of isolate K/2002 revealed an S3/S2 recombination event at the 3' end of the VP1 coding region. A recombination event resulted in the introduction of six Sabin 2 amino acid residues in a Sabin 3 genomic background. Furthermore, mutations associated with loss of the attenuated phenotype of Sabin 3 strains have been identified in the genome of isolate K/2002. The data presented here emphasize the need for careful planning of vaccination strategies, which involve stopping OPV administration in regions that are certified to be polio-free.
Dedepsidis
Evaggelos
E
Department of Biochemistry and Biotechnology, University of Thessaly, Greece.
Pliaka
Vaia
V
Kyriakopoulou
Zaharoula
Z
Brakoulias
Christos
C
Levidiotou-Stefanou
Stamatina
S
Pratti
Anastassia
A
Mamuris
Zissis
Z
Markoulatos
Panayotis
P
D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D036022:Capsid Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D002648:Child; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D017955:Poliovirus / Q000737:chemistry / Q000235:genetics*; D011055:Poliovirus Vaccine, Oral / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D011995:Recombination, Genetic
D000956:Antigens, Viral; D036022:Capsid Proteins; D011055:Poliovirus Vaccine, Oral; D011994:Recombinant Proteins