Serum proteomic analysis during nicotine self-administration, extinction and relapse in rats.
Nicotine dependence is known to induce long-term neural adaptations in brain. The purpose of this study was to verify whether specific protein patterns related to nicotine self-administration states could also be detected in a peripheral tissue. A serum proteomic analysis was performed by 2-DE on samples taken at six time points: N, naïve; P, priming; S, self-administration; W, withdrawal; E, extinction; R, relapse. After image analysis, spot volume values were submitted to a principal component analysis and relevant comparisons were selected. In N versus S; S versus W; E versus R; S versus R and S versus E comparisons a clear separation between groups could be observed, suggesting that each self-administration state correlates with a specific protein expression pattern. Partial least squares discriminant analysis was adopted to rank proteins by the contribution to the overall separation. A number of spots were identified; among them, C reactive protein and haemopexin displayed a significant reduction after nicotine administration; two haemopexin isoforms were decreased in the S state and antithrombin III was increased in the E phase. This study showed that specific protein patterns related to the nicotine self-administration states exist in serum. Further development of this approach may provide biomarkers to assess dependence states of drug-taking individuals.
Cecconi
Daniela
D
Dipartimento Scientifico e Tecnologico, University of Verona, Verona, Italy.
Tessari
Michela
M
Willé
David R
DR
Zoli
Michele
M
Domenici
Enrico
E
Righetti
Pier Giorgio
PG
Carboni
Lucia
L
D000818:Animals; D008297:Male; D009538:Nicotine / Q000008:administration & dosage*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D012646:Self Administration
D009538:Nicotine