Genetic variability of Phytophthora sojae isolates from Argentina.
Phytophthora sojae causes root and stem rot, one of the most important diseases of soybean worldwide. Genetic diversity of 32 Phytophthora sojae isolates of different geographic origin from Argentina was evaluated with RAPD markers. The isolates were collected from diseased soybean plants and soil samples from Santa Fe, Buenos Aires, C6rdoba and Entre Rios provinces, in the Pampeana Region. DNA was amplified with 20 decanucleotides primers. Seven primers amplified 49 fragments, of which 35 were polymorphic, indicating high variability. RAPD analysis detected intraspecific variability even among isolates of the same geographic origin.
Gally
Marcela
M
Fitopatología, Facultad de Agronomía, Universidad de Buenos Aires, Avenida San Martín 4453, 1417 Buenos Aires, Argentina. mgally@agro.uba.ar
Ramos
Araceli Marcela
AM
Dokmetzian
Diana
D
Lopez
Silvia Edith
SE
D001118:Argentina; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D010838:Phytophthora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012988:Soil Microbiology; D013025:Glycine max / Q000382:microbiology
D004271:DNA, Fungal