Kinetics of asthma- and allergy-associated immune response gene expression in peripheral blood mononuclear cells from vaccinated infants after in vitro re-stimulation with vaccine antigen.
The global expression of immune response genes in infants after vaccination and their role in asthma and allergy is not clearly understood. Pharmacogenomics is ideally suited to study the involved cellular responses, since the expression of thousands of genes can be assessed simultaneously. Here, array technology was used to assess the expression kinetics of immune response genes with association to asthma and allergy in peripheral blood mononuclear cells (PBMC) of five healthy infants after vaccination with Infanrix-Polio+Hib. At 12h after in vitro re-stimulation of the PBMC with pertussis toxin (PT) antigen, 14 immune response pathways, 33 allergy-related and 66 asthma-related genes were found activated.
Lahdenperä
Anne I
AI
Division of Paediatrics, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, 58185 Linköping, Sweden.
Nilsson
Lennart J
LJ
Regnström
Karin
K
D000941:Antigens / Q000276:immunology*; D001249:Asthma / Q000235:genetics* / Q000276:immunology*; D019295:Computational Biology; D015870:Gene Expression / Q000276:immunology*; D018073:Haemophilus Vaccines / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology*; D007109:Immunity / Q000235:genetics*; D007231:Infant, Newborn; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D005810:Multigene Family / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D037342:Pertussis Toxin / Q000494:pharmacology; D023321:Poliovirus Vaccines / Q000276:immunology; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D014611:Vaccination; D014612:Vaccines / Q000276:immunology*
D000941:Antigens; D018073:Haemophilus Vaccines; D023321:Poliovirus Vaccines; D014612:Vaccines; D012313:RNA; D037342:Pertussis Toxin