title: Inhibition of quorum sensing in Serratia marcescens H30 by molecular regulation.
abstract: Quorum sensing in Serratia marcescens, which uses two types of signaling molecules-N-acyl homoserine lactones and furanosyl borate diester-play important regulatory roles in the synthesis of 2,3-butanediol and prodigiosin. In the hope of understanding the effect of quorum sensing on physiologic metabolism, we established two molecular strategies, one to express acyl-homoserine lactone hydrolase to inactivate AI-1 signaling molecule using an expression vector with lactose as the inducer and the other to mutate luxS gene with a suicide plasmid pUTKm2 to inhibit the synthesis of AI-2 signaling molecule.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PRC.
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: Y L
authors.initials: YL
authors.lastname: Shen
authors.forename: D Z
authors.initials: DZ
authors.lastname: Wei
authors.forename: J W
authors.initials: JW
authors.lastname: Zhu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism; D007783:Lactones / Q000037:antagonists & inhibitors / Q000378:metabolism*; D053038:Quorum Sensing; D012706:Serratia marcescens / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D001426:Bacterial Proteins; D007783:Lactones; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria