title: Antifungal and antibacterial activities of Taxus wallichiana Zucc.
abstract: Current study was undertaken to evaluate the in vitro antifungal and antibacterial potential of methanol extract and subsequent fractions obtained after partitioning in organic solvents with variable polarity of the aerial parts of the tree Taxus wallichiana Zucc. Traditionally, this plant is often used in folk medicines in Pakistan for treating microbial infections. In order to rationalize the traditional use, methanol extracts of leaf, bark, and heartwood of Taxus wallichiana Zucc. were tested against six bacteria and six fungal strains using the Hole diffusion and macro-dilution methods. All extracts and fractions displayed significant antimicrobial effect. Only three fungal strains, Trichophyton longifusus, Microspoum canis, and Fusarium solani were susceptible to the extracts and fractions with MICs ranging from 0.08 to 200 mg/mL. In case of bacterial strains, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi were susceptible to the extracts and fractions with MICs ranging from 0.08 to 200 mg/mL. Comparison results were carried out using imipinem, miconazole and amphotericin B as standard antibiotics.
authors.affiliation: Centre of Biotechnology, University of Peshawar, Peshawar, Pakistan. akhund_54@yahoo.co.uk
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nisar
authors.forename: Inamullah
authors.initials: I
authors.lastname: Khan
authors.forename: Bashir
authors.initials: B
authors.lastname: Ahmad
authors.forename: Ihsan
authors.initials: I
authors.lastname: Ali
authors.forename: Waqar
authors.initials: W
authors.lastname: Ahmad
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D007201:Indicator Dilution Techniques; D050296:Microbial Viability / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D020946:Taxus / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010936:Plant Extracts