title: Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus.
abstract: In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
authors.affiliation: Institut für Genetik, Ludwig-Maximilians-Universität München, Maria-Ward-Str. 1a, 80638 München, Germany.
authors.forename: Takaki
authors.initials: T
authors.lastname: Maekawa
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Kusakabe
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Shimoda
authors.forename: Shusei
authors.initials: S
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tabata
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Murooka
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D017796:Caulimovirus / Q000235:genetics; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics*; D000070116:Lotus / Q000166:cytology / Q000235:genetics*; D008957:Models, Genetic; D018515:Plant Leaves / Q000235:genetics; D018517:Plant Roots / Q000235:genetics; D018547:Plant Stems / Q000235:genetics; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics; D025821:Polyubiquitin / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D053204:Root Nodules, Plant / Q000235:genetics
chemical_list: D025821:Polyubiquitin