title: Nanomechanical control of cell rolling in two dimensions through surface patterning of receptors.
abstract: We envisioned that label-free control of the transport of cells in two dimensions through receptor-ligand interactions would enable simple separation systems that are easy to implement yet retain the specificity of receptor-ligand interactions. Here we demonstrate nanomechanical control of cell transport in two dimensions via transient receptor-ligand adhesive bonds by patterning of receptors that direct cell rolling through an edge effect. HL-60 cells rolling on P-selectin receptor patterns were deflected at angles of 5-10 degrees with respect to their direction of travel. Absence of this effect in the case of rigid microsphere models of cell rolling suggests that this two-dimensional motion depends on nanomechanical properties of the rolling cell. This work suggests the feasibility of simple continuous-flow microfluidic cell separation systems that minimize processing steps and yet retain the specificity of receptor-ligand interactions.
authors.affiliation: Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Rohit
authors.initials: R
authors.lastname: Karnik
authors.forename: Seungpyo
authors.initials: S
authors.lastname: Hong
authors.forename: Huanan
authors.initials: H
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Mei
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Karp
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
mesh_terms: D018922:HL-60 Cells; D006801:Humans; D036103:Nanotechnology; D011956:Receptors, Cell Surface / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011956:Receptors, Cell Surface