title: Volumetric response of vertebrate hepatocytes challenged by osmotic gradients: a theoretical approach.
abstract: In this study we use a theoretical approach to study the volumetric response of goldfish hepatocytes challenged by osmotic gradients and compared it with that of hepatocytes from another teleost (the trout) and a mammal (the rat). Particular focus was given to the multiple non-linear interactions of transport systems enabling hypotonically challenged cells to trigger a compensatory response known as volume regulatory decrease or RVD. For this purpose we employed a mathematical model which describes the rates of change of the intracellular concentrations of main diffusible ions, of the cell volume, and of the membrane potential. The model was fitted to experimental data on the kinetics of volume change of hepatocytes challenged by anisotonic media. In trout and rat hepatocytes, experimental results had shown that hypotonic cell swelling was followed by RVD, whereas goldfish cells swelled with no concomitant RVD (M.V. Espelt et al., 2003, J. Exp. Biol. 206, 513-522). A comparison between data predicted by the model and that obtained experimentally suggests that in trout and rat hepatocytes hypotonicity activates a sensor element and this, in turn, activates an otherwise silent efflux of KCl - whose kinetics could be successfully predicted - thereby leading to volume down-regulation. In contrast, with regard to the absence of RVD in goldfish hepatocytes the model proposed suggests that either a sensor element triggering RVD is absent or that the effector mechanism (the loss of KCl) remains inactive under the conditions employed. In line with this, we recently found that extracellular nucleotides may be required to induce RVD in these cells, indicating that our model could indeed lead to useful predictions.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas (Facultad de Farmacia y Bioquímica), Universidad de Buenos Aires, C1113AAD Buenos Aires, Argentina.
authors.forename: M V
authors.initials: MV
authors.lastname: Espelt
authors.forename: K
authors.initials: K
authors.lastname: Alleva
authors.forename: G
authors.initials: G
authors.lastname: Amodeo
authors.forename: G
authors.initials: G
authors.lastname: Krumschnabel
authors.forename: R C
authors.initials: RC
authors.lastname: Rossi
authors.forename: P J
authors.initials: PJ
authors.lastname: Schwarzbaum
mesh_terms: D000818:Animals; D001692:Biological Transport; D048429:Cell Size; D005399:Fishes; D022781:Hepatocytes / Q000166:cytology*; D016623:Ion Pumps / Q000378:metabolism; D007552:Isotonic Solutions; D008024:Ligands; D008564:Membrane Potentials; D008954:Models, Biological; D009995:Osmosis; D011188:Potassium / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014714:Vertebrates / Q000378:metabolism*
chemical_list: D016623:Ion Pumps; D007552:Isotonic Solutions; D008024:Ligands; D011188:Potassium