A multivariate spot filtering model for two-dimensional gel electrophoresis.
Image segmentation plays an important role in the automatic analysis of protein spots in 2-DE. Using image segments representing protein spots, the amount of protein in each segment can be quantified, and corresponding segments can be matched and compared for multiple gels. However, the common presence of image segments caused by noise and unwanted artefacts highly disturb the analysis and comparison of the gels. Common sources of such artefacts are cracks in the gel surface, fingerprints, dust and other pollutions. It would be advantageous to remove these unwanted artefacts during or after the segmentation procedure. To achieve this task a multivariate spot filtering model is developed using image segments from a gel segmentation. Parameters in the model are based on texture, shape and intensity measurements of the image segments. The model successfully managed to separate segments caused by noise, artefacts and cracks from image segments representing true protein spots. The classification method used is discriminant partial least squares regression.
Rye
Morten Beck
MB
Department of Chemistry, Norwegian University of Science and Technology, Trondheim, Norway. mortenry@phys.chem.ntnu.no
Alsberg
Bjørn K
BK
D000818:Animals; D002138:Calibration; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D008962:Models, Theoretical; D015999:Multivariate Analysis; D009124:Muscle Proteins / Q000302:isolation & purification
D009124:Muscle Proteins