title: High-resolution, high-throughput SNP mapping in Drosophila melanogaster.
abstract: Single nucleotide polymorphisms (SNPs) are useful markers for genetic mapping experiments in model organisms. Here we report the establishment of a high-density SNP map and high-throughput genotyping assays for Drosophila melanogaster. Our map comprises 27,367 SNPs in common laboratory Drosophila stocks. These SNPs were clustered within 2,238 amplifiable markers at an average density of 1 marker every 50.3 kb, or 6.3 genes. We have also constructed a set of 62 Drosophila stocks, each of which facilitates the generation of recombinants within a defined genetic interval of 1-2 Mb. For flexible, high-throughput SNP genotyping, we used fluorescent tag-array mini-sequencing (TAMS) assays. We designed and validated TAMS assays for 293 SNPs at an average resolution of 391.3 kb, and demonstrated the utility of these tools by rapidly mapping 14 mutations that disrupt embryonic muscle patterning. These resources enable high-resolution high-throughput genetic mapping in Drosophila.
authors.affiliation: Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Chen
authors.forename: Annika
authors.initials: A
authors.lastname: Ahlford
authors.forename: Frank
authors.initials: F
authors.lastname: Schnorrer
authors.forename: Irene
authors.initials: I
authors.lastname: Kalchhauser
authors.forename: Michaela
authors.initials: M
authors.lastname: Fellner
authors.forename: Erika
authors.initials: E
authors.lastname: Viràgh
authors.forename: Istvàn
authors.initials: I
authors.lastname: Kiss
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Syvänen
authors.forename: Barry J
authors.initials: BJ
authors.lastname: Dickson
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D049750:Genome, Insect; D024510:Muscle Development / Q000235:genetics*; D009154:Mutation; D020641:Polymorphism, Single Nucleotide