Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis.
A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.
Sun
Yinghua
Y
Department of Biomedical Engineering, University of California, 451 Health Sciences Drive, Davis, California, 95616, USA.
Liu
Rui
R
Elson
Daniel S
DS
Hollars
Christopher W
CW
Jo
Javier A
JA
Park
Jesung
J
Sun
Yang
Y
Marcu
Laura
L
D004867:Equipment Design; D000431:Ethanol / Q000737:chemistry; D005454:Fluorescence Polarization; D006801:Humans; D007834:Lasers; D000432:Methanol / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D009991:Oscillometry; D012031:Refractometry; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors
D000431:Ethanol; D000432:Methanol