title: Structural insights into the elastin mimetic (LGGVG)6 using solid-state 13C NMR experiments and statistical analysis of the PDB.
abstract: Elastin is a crosslinked hydrophobic protein found in abundance in vertebrate tissue and is the source of elasticity in connective tissues and blood vessels. The repeating polypeptide sequences found in the hydrophobic domains of elastin have been the focus of many studies that attempt to understand the function of the native protein on a molecular scale. In this study, the central residues of the (LGGVG)(6) elastin mimetic are targeted. Using a combination of a statistical analysis based on structures in the Brookhaven Protein Data Bank (PDB), 1D cross-polarization magic-angle-spinning (CPMAS) NMR spectroscopy, and 2D off-magic-angle-spinning (OMAS) spin-diffusion experiments, it is determined that none of the residues are found in a singular regular, highly ordered structure. Instead, like the poly(VPGVG) elastin mimetics, there are multiple conformations and significant disorder. Furthermore, the conformational ensembles are not reflective of proteins generally, as in the PDB, suggesting that the structure distributions in elastin mimetics are unique to these peptides and are a salient feature of the functional model of the native protein.
authors.affiliation: Department of Chemistry, University of Hawaii, Honolulu, HI 96822, USA. kumashir@hawaii.edu
authors.forename: Kristin K
authors.initials: KK
authors.lastname: Kumashiro
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ohgo
authors.forename: Walter P
authors.initials: WP
authors.lastname: Niemczura
authors.forename: Allen K
authors.initials: AK
authors.lastname: Onizuka
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Asakura
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D030562:Databases, Protein; D004058:Diffusion; D004549:Elastin / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D010455:Peptides; C409505:poly(leucyl-glycyl-glycyl-valyl-glycyl); D004549:Elastin