[Expression of dopamine receptor D2 and adenosine receptor A2A in human retinal pigment epithelium].
OBJECTIVE
The aim of this study was to identify the presence of dopamine receptor D2 and adenosine receptor A2A in human retinal pigment epithelium (RPE) in order to determine whether the RPE is a potential site of action for these two receptors.


METHODS
RPE Cells were isolated and cultured. Reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry were performed to detect the expression of D2 and A2A receptors in the RPE.


RESULTS
RT-PCR analysis showed that mRNA of D2 and A2A receptors were expressed in the RPE. D2 and A2A receptors were distributed in the RPE by immunocytochemistry.


CONCLUSIONS
This study demonstrated the presence of D2 and A2A receptors in human RPE at both mRNA and protein levels. D2 and A2A receptors may play an important role in the maintenance of the function of human retinal pigment epithelium. Furthermore, their cooperation appeared to be important in the development of axial myopia and retinal and choroidal neovascularization.
Dong
Feng
F
School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou 325003, China.
An
Jian-hong
JH
Ren
Yue-ping
YP
Yan
Dong-sheng
DS
Zhou
Xiang-tian
XT
Lü
Fan
F
Hu
Dan-ning
DN
Chen
Jiang-fan
JF
Qu
Jia
J
D002478:Cells, Cultured; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000378:metabolism*; D043705:Receptor, Adenosine A2A / Q000378:metabolism*; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism; D055213:Retinal Pigment Epithelium / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D043705:Receptor, Adenosine A2A; D017448:Receptors, Dopamine D2