Implementation of a bimorph-based aperture tapping-SNOM with an incubator to study the evolution of cultured living cells.
We present the implementation of a tapping-mode aperture scanning near-field optical microscope (Tapping-SNOM) to a Binder CB incubator (Istituto di Struttura della Materia, Rome, Italy). The microscope operates in the intermittent contact mode using a nonbent optical fibre allowing to reduce the perturbation exerted on the sample, while the incubator maintains a constant temperature, humidity and CO(2) level. This instrument can maintain and analyse in a controlled environment different samples, both organic and nonorganic. In particular, the Tapping-SNOM can study different cell lines at nanometric resolution and in physiological buffer, following the evolution of the living cells almost indefinitely. We will present several examples of the capabilities of the tapping scanning near-field optical microscope in the study of different lines of living cells, showing corresponding topographical, optical or phase-lag images of the live samples, evidencing the excellent stability, versatility and resolution of the system.
Longo
G
G
Istituto di Struttura della Materia, CNR, Via fosso del Cavaliere 100, 00133 Roma, Italy. longo@ism.cnr.it
Girasole
M
M
Cricenti
A
A
D000818:Animals; D045744:Cell Line, Tumor / Q000648:ultrastructure; D002478:Cells, Cultured / Q000648:ultrastructure; D042783:Endothelial Cells / Q000502:physiology* / Q000648:ultrastructure; D004867:Equipment Design; D004912:Erythrocytes / Q000502:physiology* / Q000648:ultrastructure; D005336:Fiber Optic Technology; D006801:Humans; D015603:Keratinocytes / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods*; D020527:Microscopy, Scanning Probe / Q000295:instrumentation; D009447:Neuroblastoma; D013552:Swine