title: Pseudomonas japonica sp. nov., a novel species that assimilates straight chain alkylphenols.
abstract: A bacterial strain, WL(T), which was isolated from an activated sludge, was able to degrade alkylphenols. 16S rDNA sequence analysis indicated that strain WL(T) belonged to the genus Pseudomonas (sensu stricto) and formed a monophyletic clade with the type strain of Pseudomonas graminis and other members in the Pseudomonas putida subcluster with sequence similarity values higher than 97%. Genomic relatedness based on DNA-DNA hybridization of strain WL(T) to these strains is 2-41%. Strain WL(T) contained ubiquinone-9 as the main respiratory quinone, and the G+C content of DNA was 66 mol%. The organism contained hexadecanoic acid (16:0), hexadecenoic acid (16:1) and octadecenoic acid (18:1) as major cellular fatty acids. The hydroxy fatty acids detected were 3-hydroxydecanoic acid (3-OH 10:0), 3-hydroxydodecanoic acid (3-OH 12:0) and 2-hydroxydodecanoic acid (2-OH 12:0). These results, as well as physiological and biochemical characteristics clearly indicate that the strain WL(T) represents a new Pseudomonas species, for which the name Pseudomonas japonica is proposed. The type strain is strain WL(T) (=IAM 15071T=TISTR 1526T).
authors.affiliation: Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Wiboonluk
authors.initials: W
authors.lastname: Pungrasmi
authors.forename: Haeng-Seog
authors.initials: HS
authors.lastname: Lee
authors.forename: Akira
authors.initials: A
authors.lastname: Yokota
authors.forename: Akinori
authors.initials: A
authors.lastname: Ohta
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism*; D010641:Phenotype; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity; D014041:Tokyo
chemical_list: D005227:Fatty Acids; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012722:Sewage