beta(2)-Adrenergic receptor and adenylate cyclase gene polymorphisms affect sickle red cell adhesion.
Sickle red cell (SS RBC) adhesion is thought to contribute to sickle cell disease (SCD) pathophysiology. SS RBC adhesion to laminin increases in response to adrenaline stimulation of beta(2)-adrenergic receptors (beta(2)ARs) and adenylate cyclase (ADCY6), and previous evidence suggests such activation occurs in vivo. We explored whether polymorphisms of the beta(2)AR and ADCY6 genes (ADRB2 and ADCY6, respectively) affect RBC adhesion to laminin. We found that the beta(2)AR arg(16)-->gly substitution and two non-coding ADCY6 polymorphisms were associated with elevated adhesion. We postulate that ADRB2 and ADCY6 polymorphisms may influence SCD severity through the mechanism of RBC adhesion.
Eyler
Christine E
CE
Division of Hematology and Centre for Human Genetics, Department of Medicine, Duke Comprehensive Sickle Cell Centre, Duke University, Durham, NC 27710, USA.
Jackson
Terry
T
Elliott
Laine E
LE
De Castro
Laura M
LM
Jonassaint
Jude
J
Ashley-Koch
Allison
A
Telen
Marilyn J
MJ
D000262:Adenylyl Cyclases / Q000235:genetics*; D000755:Anemia, Sickle Cell / Q000097:blood / Q000235:genetics*; D002448:Cell Adhesion / Q000235:genetics; D004912:Erythrocytes / Q000378:metabolism; D005838:Genotype; D006801:Humans; D007797:Laminin / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*
D007797:Laminin; D018343:Receptors, Adrenergic, beta-2; D000262:Adenylyl Cyclases