Human vesicular glutamate transporters functionally complement EAT-4 in C. elegans.
The vesicular glutamate transporter (VGLUT) transports glutamate into pre-synaptic vesicles. Three isoforms of VGLUT have been identified in humans, but their functional differences remain largely unknown. EAT-4 is the only homologue of human VGLUT in C. elegans. Here we report that mutants of eat-4 exhibit hyperforaging behavior and that each of the isoforms of human VGLUT functionally rescues the defects in eat-4 worms.
Lee
Dukgyu
D
IBST/Graduate Program in Neuroscience, Inje University, Busan 614-735, Korea.
Jung
Sunki
S
Ryu
Jungmin
J
Ahnn
Joohong
J
Ha
Ilho
I
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000166:cytology / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D017470:Receptors, Glutamate / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D050496:Vesicular Glutamate Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D029742:Caenorhabditis elegans Proteins; C500224:Eat-4 protein, C elegans; D020033:Protein Isoforms; D017470:Receptors, Glutamate; D011993:Recombinant Fusion Proteins; D050496:Vesicular Glutamate Transport Proteins