Cloning and characterization of a heat shock protein 70 gene, MsHSP70-1, in Medicago sativa.
Some members of the heat shock protein 70 (HSP70) family have important functions in organism development. Here, we identified an alfalfa (Medicago sativa L. cv. Algonquin) HSP70 gene, MsHSP70-1, using cDNA array and reverse transcription-polymerase chain reaction. This gene contains a 1947 bp open reading frame encoding a protein of 649 amino acids. This protein contains all conserved domains, motifs, and characteristic sequences of plant HSP70s. The expression of MsHSP70-1 is enhanced in nodule compared with root, stem, leaf, and flower, and throughout the process of nodule development. Northern hybridization analysis indicated that the expression of MsHSP70-1 in nodule requires the active bacA gene of rhizobia. These results suggested that MsHSP70-1 might play an important role in alfalfa nodule development.
He
Zhishui
Z
National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Xie
Rong
R
Wang
Yanzhang
Y
Zou
Huasong
H
Zhu
Jiabi
J
Yu
Guanqiao
G
D003001:Cloning, Molecular; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000455:Medicago sativa / Q000235:genetics / Q000378:metabolism*; D018514:Plant Structures / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D018840:HSP70 Heat-Shock Proteins