title: Phycicoccus dokdonensis sp. nov., isolated from soil.
abstract: A Gram-positive, non-motile, coccoid-shaped bacterium, designated strain DS-8(T), was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic study. Strain DS-8(T) grew optimally at 30 degrees C and pH 6.5-7.5 in the presence of 0.5-1.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DS-8(T) joined the type strain of Phycicoccus jejuensis, with a bootstrap resampling value of 92.5 %, and shared 96.5 % 16S rRNA gene sequence similarity with P. jejuensis. The cell-wall peptidoglycan type was based on meso-diaminopimelic acid and the acyl type of the muramic acid was acetyl. The predominant menaquinone was MK-8(H(4)). The major fatty acids were iso-C(15 : 0) and iso-C(16 : 0). Major polar lipids detected in strain DS-8(T) were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 70.7 mol%. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain DS-8(T) differs from P. jejuensis. On the basis of the data obtained, strain DS-8(T) is considered to represent a novel species of the genus Phycicoccus, for which the name Phycicoccus dokdonensis sp. nov. is proposed. The type strain is DS-8(T) (=KCTC 19248(T)=CCUG 54521(T)).
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.re.kr
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kang
authors.forename: Tae-Kwang
authors.initials: TK
authors.lastname: Oh
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S