title: [Determination of HPLC fingerprint of Qingfengtong capsule].
abstract: OBJECTIVE
  To establish a fingerprint analysis method of Qingfengtong capsule by HPLC.
  
  
  METHODS
  The samples were extracted with 70% ethanol in an ultrasonic bath. The extracts were analyzed at 35 degrees C on a Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with 0.1 mol x L(-1) potassium dihydrogen phosphate water-solution as mobile phase A, and methanol as mobile phase B. The analysis followed a linear gradient elution program. Initial condition of the mobile phase was 10% B for 2 minutes; then changed to 90% B in 40 minutes. The flow rate was kept at 1.0 ml x min(-1) and the detector wavelength was 262 nm.
  
  
  RESULTS
  The main peaks in fingerprint chromatogram of Qingfengtong capsules were separated fairly well. The results of method validation meet the requirements for the fingerprints.
  
  
  CONCLUSIONS
  The established method can be used for the quality control of Qingfengtong capsules.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Yi
authors.forename: Gui-Pin
authors.initials: GP
authors.lastname: Chen
mesh_terms: D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification / Q000592:standards; D034181:Epimedium / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry; D032841:Sinomenium / Q000737:chemistry
chemical_list: D002214:Capsules; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C539471:qingfengtong