title: A plate holder for non-destructive testing of mesophase crystallization assays.
abstract: Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.
authors.affiliation: Unidad de Biofísica (CSIC-UPV/EHU), Bilbao, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Agirre
authors.forename: A
authors.initials: A
authors.lastname: Mechaly
authors.forename: A
authors.initials: A
authors.lastname: Cabo-Bilbao
authors.forename: D M A
authors.initials: DM
authors.lastname: Guérin
mesh_terms: D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D008422:Materials Testing / Q000379:methods*; D044367:Phase Transition; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D013048:Specimen Handling / Q000379:methods*; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D011506:Proteins