Effects of inorganic nutrient levels on the biodegradation of benzene, toluene, and xylene (BTX) by Pseudomonas spp. in a laboratory porous media sand aquifer model.
The effect of inorganic nutrients (sulfate, phosphate, and ammonium chloride) on the aerobic biodegradation of benzene, toluene, and xylene (BTX) by Pseudomonas spp. was studied in the laboratory using a glass sand tank. The increase of nutrient levels resulted in enhanced bacterial growth and BTX degradation. Sulfate and phosphate serve as key electron acceptors in the microbiological processes degrading BTX. The observed bacterial morphological changes during BTX degradation reveal that the filamentous bacteria were the dominant species at low temperatures about 20 degrees C. The spherical and rod-shaped cells became dominant at higher temperatures ranging from 25 degrees C to 28 degrees C. When the BTX mixture was allowed to be biodegraded for longer incubation periods of 21-42 h at high phosphate concentrations, large amounts of rod-shaped cells were clustered. The morphological adaptation appears to be controlled by the temperature and nutrient levels in the sandy medium where Pseudomonas spp. thrives.
Jean
Jiin-Shuh
JS
Department of Earth Sciences, National Cheng Kung University, Tainan City 70101, Taiwan. jiinshuh@mail.ncku.edu.tw
Lee
Ming-Kuo
MK
Wang
Shih-Ming
SM
Chattopadhyay
Pabitra
P
Maity
Jyoti Prakash
JP
D000332:Aerobiosis; D000643:Ammonium Chloride / Q000494:pharmacology; D001483:Base Sequence; D001554:Benzene / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D004269:DNA, Bacterial / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D005502:Food; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010710:Phosphates / Q000494:pharmacology; D016062:Porosity; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000378:metabolism*; D013431:Sulfates / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014050:Toluene / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism
D004269:DNA, Bacterial; D010710:Phosphates; D013431:Sulfates; D014992:Xylenes; D000643:Ammonium Chloride; D014050:Toluene; D012822:Silicon Dioxide; D001554:Benzene