title: Purification and characterization of a rice class I chitinase, OsChia1b, produced in Esherichia coli.
abstract: To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, 8050 Ikarashi-2, Niigata 950-2181, Japan.
authors.forename: Ryoji
authors.initials: R
authors.lastname: Mizuno
authors.forename: Yoshikane
authors.initials: Y
authors.lastname: Itoh
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishizawa
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kezuka
authors.forename: Kazushi
authors.initials: K
authors.lastname: Suzuki
authors.forename: Takamasa
authors.initials: T
authors.lastname: Nonaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D029968:Escherichia coli Proteins / Q000235:genetics; D025301:Hyphae / Q000254:growth & development; D012275:Oryza / Q000201:enzymology*; D011487:Protein Conformation; D012995:Solubility; D013379:Substrate Specificity; D014242:Trichoderma / Q000276:immunology / Q000648:ultrastructure
chemical_list: D029968:Escherichia coli Proteins; D002686:Chitin; D002688:Chitinases; C094590:chitinase ChiA