[Experimental study of Gukangling Decoction combined with technetium [99Tc] methylene diphosphonate injection in treating osteoporotic rabbits].
OBJECTIVE
To evaluate the advantage of Gukangling Decoction (GKLD), a compound traditional Chinese herbal medicine, combined with technetium [(99)Tc] methylene diphosphonate injection ((99)Tc-MDP) in treating osteoporosis in rabbits.


METHODS
A rabbit model of osteoporosis was established by intramuscular injection of dexamethasone (DX). Fifty-six rabbits were divided into 8 groups: Group A, B, C, D, E, F, G and H. Rabbits in groups A and B were intramuscularly injected normal saline as normal control, groups C and D were untreated groups, rabbits in group E were treated by (99)-MDP, rabbits in group F were treated by aminodiphosphate, rabbits in group G were treated by GKLD, and rabbits in group H were treated by (99)-MDP and GKLD. Rabbits in groups A and C were executed to demonstrate the establishment of the rabbit model of osteoporosis at the 8th week of experiment. Rabbits in the other six groups were executed after 16-week experiment (8-week treatment), and then bone structure and cell shape were observed by electron microscope, X-ray, CT and emission computed tomography (ECT). Bone density, biomechanical parameters, the levels of bone specific alkaline phosphatase (BALP) and bone Gla protein (BGP) were measured too.


RESULTS
After 8-week of intramuscular injection of DX, the bone trabecula in group A were regular and showed normal configuration, while the bone trabecula in group C were sparse, ruptured and showed damaged form. The bone density and biomechanical parameters in group A were higher than those in group C, indicating that the rabbit model of osteoporosis was established successfully. At the 9th week of experiment, the results of cell pathology in group D showed that the bone trabeculas were sparse, ruptured, defected or had hollow section, but the bone trabeculas in group B were regular and dense. The bone trabeculas in groups H and E were restored, and were thicker than those in group D. The bone quality in groups H and E was better than group D significantly, the bone quality in group F was better than group G, and the bone quality in group G was better than group D slightly.


CONCLUSIONS
GKLD combined with (99)-MDP had superiority in treating osteoporosis of rabbits as compared with the respective single therapy.
Zhao
Guo-ding
GD
Department of Traditional Chinese Internal Medicine, Central Hospital of Huangpu District, Shanghai 200002, China. chenghualong@126.com
Gao
Ke-jia
KJ
Tian
Ying-min
YM
Ye
Zhi-wei
ZW
Li
Wei
W
Song
Hai-ping
HP
Cai
Zheng-yu
ZY
D000818:Animals; D003907:Dexamethasone; D004359:Drug Therapy, Combination; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D008297:Male; D010024:Osteoporosis / Q000139:chemically induced / Q000188:drug therapy*; D008517:Phytotherapy; D011817:Rabbits; D013669:Technetium Tc 99m Medronate / Q000627:therapeutic use*
D004365:Drugs, Chinese Herbal; D003907:Dexamethasone; D013669:Technetium Tc 99m Medronate