[The role of OX40 in CD4+ T cells cytokines production in ulcerative colitis].
OBJECTIVE
To investigate the expression of OX40 on CD(4)(+) T cells in patients with ulcerative colitis (UC) and the role of OX40/OX40L interaction for the cytokine production of lamina propria (LP)-CD(4)(+) T cells from UC.


METHODS
LP-CD(4)(+) T cells were purified. The expression of OX40 molecule was measured with FACS. LP-CD(4)(+) T cells were cultured with different stimuli and proliferation was assessed. The cytokines concentrations of the culture supernatant were detected.


RESULTS
No difference of the OX40 expression was observed among the CD(4)(+) T cells from peripheral blood (PB) of UC patients, LP of non-inflammatory colonic tissue in UC patients and control PB. However, the expression of OX40 was significantly higher on LP-CD(4)(+) T cells from inflammatory colonic tissue in UC patients. In vitro culture with antigen presenting cells, the levels of IFNgamma and TNFalpha secreted by LP-CD(4)(+) T cells from the inflammatory colonic tissue were significantly higher than those from the non-inflammatory colonic tissue (both P < 0.01). The levels of IFNgamma and TNFalpha secreted by LP-CD(4)(+) T cells from the inflammatory colonic tissue were further increased by anti-OX40 MoAb stimulation, but suppressed significantly by adding anti-OX40L MoAb (compared with non stimulation, P < 0.01, respectively). The IFNgamma and TNFalpha secretion of the LP-CD(4)(+) T cells from the non-inflammatory colonic tissue were not significantly different with and without anti-OX40 or anti-OX40L MoAbs stimulation. IL-4 and IL-10 produced by LP-CD(4)(+) T cells from the inflammatory or non-inflammatory colonic tissue were not significantly changed when adding different stimuli.


CONCLUSIONS
OX40 is highly expressed on LP-CD(4)(+) T cells from inflammatory colonic tissue in patients with UC. Anti-OX40L MoAb can inhibit the proinflammatory cytokines secreted by these cells. It is indicated that OX40(+) T cells are involved in the immunopathological process in UC and blockage of the interaction of OX40 and OX40L is a new strategy to be considered for the treatment of the disease.
Wang
Xiao-di
XD
Department of Gastroenterology, China-Japan Friendship Hospital, Beijing 100029, China. xdwang60@hotmail.com
Wu
Tie-Yong
TY
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008297:Male; D053278:OX40 Ligand / Q000096:biosynthesis* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D053278:OX40 Ligand; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma