title: Versatile microbial surface-display for environmental remediation and biofuels production.
abstract: Surface display is a powerful technique that uses natural microbial functional components to express proteins or peptides on the cell exterior. Since the reporting of the first surface-display system in the mid-1980s, a variety of new systems have been reported for yeast, Gram-positive and Gram-negative bacteria. Non-conventional display methods are emerging, eliminating the generation of genetically modified microorganisms. Cells with surface display are used as biocatalysts, biosorbents and biostimulants. Microbial cell-surface display has proven to be extremely important for numerous applications, ranging from combinatorial library screening and protein engineering to bioremediation and biofuels production.
authors.affiliation: Lawrence Berkeley National Laboratory, One Cyclotron Road. MS 70A-3317, Berkeley, CA 94720, USA.
authors.forename: Cindy H
authors.initials: CH
authors.lastname: Wu
authors.forename: Ashok
authors.initials: A
authors.lastname: Mulchandani
authors.forename: Wilfred
authors.initials: W
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D052918:Environmental Restoration and Remediation / Q000379:methods*; D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D000440:Butanols; D005656:Fungal Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D000431:Ethanol