SNOM on cell thin sections: observation of Jurkat and MDAMB453 cells.
In this study a comparison of scanning near-field optical microscopy with a traditional, well-known microscopic technique like transmission electron microscopy is discussed. To establish a reliable and comparable method for high-resolution scanning near-field optical microscopy imaging of biological samples, the attention is focussed on cell sections. In particular, we present a study of ultrathin sections of Jurkat T-cells and MDAMB453 cells. We show the relationship among the scanning near-field optical microscopy (topographic and optical) images and the kind of embedding medium (resin), the sections thickness and the staining of the sample. For a complementary investigation atomic force microscopy measurements are carried out, as well. The study reveals that scanning near-field optical microscopy technique on opportunely prepared thin sections can be applied successfully for investigation of the interior of the cells. Scanning near-field optical microscopy and transmission electron microscopy allow to obtain different, however comparable, and complementary information of the cell sample.
Zweyer
M
M
Dipartimento Universitario Clinico di Biomedicina, University of Trieste, Italy. zweyer@univ.trieste.it
Troian
B
B
Spreafico
V
V
Prato
S
S
D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D004853:Epoxy Resins; D005260:Female; D006801:Humans; D019169:Jurkat Cells / Q000166:cytology / Q000648:ultrastructure*; D042361:Mammary Glands, Human / Q000166:cytology / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D020527:Microscopy, Scanning Probe / Q000379:methods*; D008867:Microtomy / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods
D004853:Epoxy Resins