Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma.
Circulating nucleic acids have been shown to have potential as non-invasive diagnostic markers in cancer. We therefore investigated whether microRNAs also have diagnostic utility by comparing levels of tumour-associated MIRN155 (miR-155), MIRN210 (miR-210) and MIRN21 (miR-21) in serum from diffuse large B-cell lymphoma (DLBCL) patients (n = 60) with healthy controls (n = 43). Levels were higher in patient than control sera (P = 0.009, 0.02 and 0.04 respectively). Moreover, high MIRN21 expression was associated with relapse-free survival (P = 0.05). This is the first description of circulating microRNAs and suggests that microRNAs have potential as non-invasive diagnostic markers for DLBCL and possibly other cancers.
Lawrie
Charles H
CH
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK. charles.lawrie@ndcls.ox.ac.uk
Gal
Shira
S
Dunlop
Heather M
HM
Pushkaran
Beena
B
Liggins
Amanda P
AP
Pulford
Karen
K
Banham
Alison H
AH
Pezzella
Francesco
F
Boultwood
Jacqueline
J
Wainscoat
James S
JS
Hatton
Christian S R
CS
Harris
Adrian L
AL
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D005260:Female; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012334:RNA, Neoplasm / Q000097:blood*; D012189:Retrospective Studies; D016019:Survival Analysis
D014408:Biomarkers, Tumor; D035683:MicroRNAs; D012334:RNA, Neoplasm