title: Lysine 263 residue of NPM/B23 is essential for regulating ATP binding and B23 stability.
abstract: Here, we show that Nucleophsomin/B23 provides lysine 263 as a critical binding site for ATP. Mutagenesis of lysine 263 to asparagine (K263N) disrupts B23 from ATP binding. While B23 WT exclusively localizes to the nucleolus, the B23-K263N is redistributed from the nucleolus to the nucleoplam. Notably, the K263N mutant is unstable, and displayed rapid degradation. Alteration of K263 induced B23 instability through increased ubiquitination and proteaosomal degradation. Moreover, mutation of K263 impedes the mitogenic effect of B23 in PC12 cells. Thus, K263 is a critical site for ATP binding and required for B23 stability, confining B23 in the nucleolus.
authors.affiliation: Departments of Molecular Cell Biology, Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea.
authors.forename: Joung Woo
authors.initials: JW
authors.lastname: Choi
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Lee
authors.forename: Chung Kwon
authors.initials: CK
authors.lastname: Kim
authors.forename: Kyung-Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Jee-Yin
authors.initials: JY
authors.lastname: Ahn
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002466:Cell Nucleolus / Q000737:chemistry; D049109:Cell Proliferation; D008239:Lysine / Q000737:chemistry* / Q000235:genetics; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000090243:Nucleophosmin; D016716:PC12 Cells; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D051381:Rats; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D025801:Ubiquitin; D000090243:Nucleophosmin; D000255:Adenosine Triphosphate; D046988:Proteasome Endopeptidase Complex; D008239:Lysine