Alterations of erythrocyte structure and cellular susceptibility in patients with chronic renal failure: effect of haemodialysis and oxidative stress.
The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H(2)O(2) among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H(2)O(2) (p<0.005) and at 60 min of haemodialysis (p<0.05), (2) significant decrease of cytoskeletal protein-protein interactions (p<0.005) and (3) cellular osmotic fragility (p<0.0005). H(2)O(2) exacerbated these changes. Erythrocytes from CRF patients have changed rheological behaviour and present higher susceptibility to disintegration. Erythrocytes membrane characteristics indicate that CRF patients possess younger and more flexible cells, which are more susceptible to oxidative stress. This may contribute to the shortened survival of young erythrocytes in CRF patients.
Brzeszczynska
Joanna
J
School of Life Sciences, Heriot-Watt University, Edinburgh, UK. joanna.brzeszczynska@hw.ac.uk
Luciak
Marek
M
Gwozdzinski
Krzysztof
K
D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018056:Hemorheology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007676:Kidney Failure, Chronic / Q000097:blood / Q000378:metabolism / Q000628:therapy*; D008560:Membrane Fluidity / Q000187:drug effects; D008875:Middle Aged; D009996:Osmotic Fragility / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D006435:Renal Dialysis; D013997:Time Factors
D003598:Cytoskeletal Proteins; D016877:Oxidants; D006861:Hydrogen Peroxide