Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts.
Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol.
Debacq-Chainiaux
Florence
F
Unit of Research on Cellular Biology, Department of Biology, University of Namur, Rue de Bruxelles, B-5000 Namur, Belgium.
Pascal
Thierry
T
Boilan
Emmanuelle
E
Bastin
Coralie
C
Bauwens
Emilie
E
Toussaint
Olivier
O
D002460:Cell Line; D016922:Cellular Senescence; D004171:Diploidy; D000431:Ethanol / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D018972:Insulin-Like Growth Factor Binding Protein 3; D000431:Ethanol; D020122:tert-Butylhydroperoxide