title: [Studies on the methods of extract and determination of total tannins and gallic acids of the root of Euphorbia hylonoma].
abstract: OBJECTIVE
  To determine total tannins and gallic acid of the root of Euphorbia hyloomla.
  
  
  METHODS
  With the gallic acid as reference, tannins and gallic acid of the root of Euphorbia hylonoma were determined by ultraviolet spectrophtometer and high performance liquid chromatography, respectively.
  
  
  RESULTS
  The content of tannins of the root of Euphorbia hylonoma were determined by different extract methods, including extracted by water, 70% alcohol, acetone on supersound, the determination data were 4.375%, 7.240%, 3.958%, respectively. When used recirculation method by water, 70% alcohol, acetone, the determination data were the determination data are 3.773%, 2.503%, 1.59%, respectively. The content of gallic acid of the root was 0.047%.
  
  
  CONCLUSIONS
  Content of total tannins by alcohol super sound is the highest comparing with other extract methods, this method can used in extract of total Tannins of the root of Euphorbia hylonoma.
authors.affiliation: Faculty of Pharmacy of School of Medicine, Xian Jiao-tong University, Xihn 710061, China. guozj@mail.xjtu.edu.cn
authors.forename: Zeng-jun
authors.initials: ZJ
authors.lastname: Guo
authors.forename: Qi-shi
authors.initials: QS
authors.lastname: Sun
authors.forename: Li-hui
authors.initials: LH
authors.lastname: Long
authors.forename: Xiao-qian
authors.initials: XQ
authors.lastname: Bu
mesh_terms: D000096:Acetone; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol; D028481:Euphorbia / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013634:Tannins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D013634:Tannins; D000096:Acetone; D000431:Ethanol; D005707:Gallic Acid