Effects of four carbamate compounds on antioxidant parameters.
The effect of four carbamates, aldicarb and its metabolites (aldicarb sulfone and aldicarb sulfoxide) and propoxur on glutathione content and the activity of the enzymes involved in the sulfur-redox cycle in the mammalian cellular model CHO-K1 cells after 24-h exposure were determined. Carbamate exposure resulted in a depletion of intracellular reduced glutathione (GSH) content, no change was observed in oxidized glutathione (GSSG) and a decrease in GSH/GSSG ratio was detected. After carbamates exposition a GSH/GSSG decreases in ranged from 12.44% to 21.35% of control was observed. Depletion of GSH levels was accompanied by the induction of glutathione reductase (GR) after 24h exposure with each of the four carbamates to CHO-K1 cells. After aldicarb sulfone, aldicarb sulfoxide, and propoxur exposure, glutathione peroxidase (GPx) activity increased in CHO-K1 cells by 198%, 32%, and 228% of control, respectively. After aldicarb sulfone and propoxur exposure, glutathione transferase (GST) activities increased by 49% and 230% of control, respectively. Due to the role played by GSH in preventing cytotoxicity via free-radical scavenging, results obtained suggest that high concentrations of aldicarb sulfone and propoxur closely resembling oxidative stress in CHO-K1 cells.
Maran
Elisa
E
Sezione di Chimica Analitica e Ambientale, Dip. Scienze Chimiche, Universita degli Studi di Trieste, Italy.
Fernández
Mónica
M
Barbieri
Pierluigi
P
Font
Guillermina
G
Ruiz
María José
MJ
D000448:Aldicarb / Q000031:analogs & derivatives* / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000502:physiology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004790:Enzyme Induction; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D001074:Propoxur / Q000633:toxicity*
D000975:Antioxidants; D007306:Insecticides; C030579:aldicarb sulfoxide; D000448:Aldicarb; D001074:Propoxur; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; C030580:aldicarb sulfone; D019803:Glutathione Disulfide