title: Effect of xylitol on adhesion of Salmonella Typhimurium and Escherichia coli O157:H7 to beef carcass surfaces.
abstract: Effects of 10% xylitol (a five-carbon sugar alcohol) on adhesion of Escherichia coli O157:H7 and Salmonella Typhimurium to meat surfaces were examined with three approaches. First, beef outside round was inoculated with rifampin-resistant E. coli O157:H7 and Salmonella Typhimurium dispersed in xylitol or peptone solution. Samples were rinsed with water or not rinsed in a 2 x 2 factorial arrangement. No interaction existed between inoculum and rinsing treatments (P > 0.84). Incubation in xylitol had minimal impact on pathogen adhesion (P > 0.76); however, rinsing reduced pathogen cell counts (P < 0.01). Second, meat samples were treated with water, xylitol, or no rinse; inoculated with pathogens dispersed in peptone solution (8.6 log CFU/ml for each pathogen); and then treated with water, xylitol, or no rinse in a 3 x 3 factorial arrangement. No interactions were observed (P > 0.50). Postinoculation rinsing reduced pathogen loads (P < 0.01) without difference between water and xylitol (P > 0.64). Third, carcass surfaces inoculated with pathogens (5.5 log CFU/cm2) were treated with 35 degrees C water wash, 2.5% L-lactic acid spray, 10% xylitol spray, lactic acid plus xylitol, or hot water plus xylitol. Lactic acid treatments reduced Salmonella Typhimurium at 0 h (P < 0.01) and 24 h (P < 0.02). Hot water treatments tended to reduce Salmonella Typhimurium at 0 h (P < 0.07). Xylitol did not reduce pathogens (P > 0.62) or increase effectiveness of other treatments. Xylitol does not influence E. coli O157:H7 and Salmonella Typhimurium adhesion to meat surfaces.
authors.affiliation: Department of Animal Science, Texas A&M University, 2471 TAMU, College Station, Texas 77843-2471, USA. j-sawyer@tamu.edu
authors.forename: J E
authors.initials: JE
authors.lastname: Sawyer
authors.forename: S T
authors.initials: ST
authors.lastname: Greiner
authors.forename: G R
authors.initials: GR
authors.lastname: Acuff
authors.forename: L M
authors.initials: LM
authors.lastname: Lucia
authors.forename: E
authors.initials: E
authors.lastname: Cabrera-Diaz
authors.forename: D S
authors.initials: DS
authors.lastname: Hale
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle / Q000382:microbiology*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D019453:Escherichia coli O157 / Q000187:drug effects / Q000502:physiology*; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling / Q000379:methods; D005516:Food Microbiology; D006801:Humans; D012486:Salmonella typhimurium / Q000187:drug effects / Q000502:physiology*; D012499:Sanitation; D013549:Sweetening Agents / Q000494:pharmacology; D014993:Xylitol / Q000494:pharmacology*
chemical_list: D013549:Sweetening Agents; D014993:Xylitol