Analysis of selective production of Nalpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde and Nalpha-benzyloxycarbonyl-L-aminoadipic acid by Rhodococcus sp. AIU Z-35-1.
The factors for selective production of N(alpha)-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde (N(alpha)-Z-L-AASA) and N(alpha)-benzyloxycarbonyl-L-aminoadipic acid (N(alpha)-Z-L-AAA) from N(alpha)-benzyloxycarbonyl-L-lysine (N(alpha)-Z-L-lysine) by Rhodococcus sp. AIU Z-35-1 were analyzed. The cultivation time was important for selective production of N(alpha)-Z-L-AASA, since N(alpha)-Z-L-lysine oxidizing enzyme reached maximum at the early stage of cell growth and then decreased. The differences of enzyme activities of N(alpha)-Z-L-lysine oxidizing enzyme and N(alpha)-Z-L-AASA dehydrogenase in pH and temperature also affected the selective production of N(alpha)-Z-L-AASA. For efficient production of N(alpha)-Z-L-AAA, it was important for cultivation time that N(alpha)-Z-L-AASA dehydrogenase activity be higher than N(alpha)-Z-L-lysine oxidizing enzyme activity, since a high concentration of N(alpha)-Z-L-AASA inhibited N(alpha)-Z-L-AASA dehydrogenase activity. The optimum pH of N(alpha)-Z-L-AAA production was affected by the instability of N(alpha)-Z-L-AASA dehydrogenase in the alkaline pH region.
Isobe
Kimiyasu
K
Department of Agro-bioscience, Faculty of Agriculture, Iwate University, 3 Ueda, Morioka 020-8550, Japan. kiso@iwate-u.ac.jp
Fukuda
Nahoko
N
Nagasawa
Shouko
S
D015074:2-Aminoadipic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D012240:Rhodococcus / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity
D001426:Bacterial Proteins; C526190:N(alpha)-benzyloxycarbonyl-aminoadipic acid; C505609:N(alpha)-benzyloxycarbonylaminoadipic acid delta-semialdehyde; D015074:2-Aminoadipic Acid; D000587:Oxidoreductases Acting on CH-NH Group Donors; C109029:delta-1-piperideine-6-carboxylate dehydrogenase