title: [Effect of short interfering RNAs on hepatitis C Virus 5' untranslated region expression].
abstract: OBJECTIVE
  To evaluate interfering effect of several short interfering RNAs (siRNA) on HCV 5' untranslated region(5' UTR).
  
  
  METHODS
  The green fluorescent protein (GFP) was used as reporter gene. A fused gene of HCV-5'UTR and GFP was constructed. It was cloned into the plasmid pCDNA3.1 named as pcDNA-HCV-5'UTR-GFP. Three siRNAs were designed and transfected into HepG2 cells with pcDNA-HCV-5'UTR-GFP. The change of the fluorescence intensity of HepG2 cells was shown by fluorescence microscopy and numerically detected under 488 nm wave length by flow cytometry.
  
  
  RESULTS
  The fused gene of HCV-5'UTR and GFP was successfully constructed. The seven groups displayed inhibitory effects on the gene expression of GFP. The inhibition rates of siRNA A, B and C were 68.4 percent, 72.6 percent and 75.6 percent, respectively. The inhibitory rates of siRN A + B, siRN B +C and siRN A +C were 91.8 percent, 87.2 percent and 92.4 percent, respectively. The inhibitory rates of siRN A+B +C was the highest, up to 95.7 percent.
  
  
  CONCLUSIONS
  These siRNAs could inhibit expression of HCV 5'UTR gene, the inhibitory effect of combined siRNA was better than that of single siRNAs.
authors.affiliation: Clinical Laboratory Center, Hangzhou Second Hospital, Hangzhou 310015, China. hzczj2006@163.com
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Chen
authors.forename: La-hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D020121:5' Untranslated Regions; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins