title: Identification of an N-linked glycosylation in the C4 region of HIV-1 envelope gp120 that is critical for recognition of neighboring CD4 T cell epitopes.
abstract: The heavy glycosylation of HIV-1 envelope gp120 shields this important Ag from recognition by neutralizing Abs and cytolytic CD8 T cells. However, very little work has been done to understand the influence of glycosylation on the generation of gp120 epitopes and their recognition by MHC class II-restricted CD4 T cells. In this study, three conserved glycans (linked to N406, N448, and N463) flanking the C4 region of gp120 that contains many known CD4 T cell epitopes were disrupted individually or in combination by asparagine-to-glutamine substitutions. The mutant proteins lacking the N448 glycan did not effectively stimulate CD4 T cells specific for the nearby C4 epitopes, although the same mutants were recognized well by CD4 T cells specific for epitopes located in the distant C1 and C2 regions. The loss of recognition was not due to amino acid substitutions introduced to the mutant proteins. Data from trypsin digestion and mass spectrometry analyses demonstrated that the N448 glycan removal impeded the proteolytic cleavage of the nearby C4 region, without affecting more distant sites. Importantly, this inhibitory effect was observed only in the digestion of the native nondenatured protein and not in that of the denatured protein. These data indicate that the loss of the N448 glycan induces structural changes in the C4 region of gp120 that make this specific region more resistant to proteolytic processing, thereby restricting the generation of CD4 T cell epitopes from this region. Hence, N-linked glycans are critical determinants that can profoundly influence CD4 T cell recognition of HIV-1 gp120.
authors.affiliation: Department of Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, USA.
authors.forename: Hualin
authors.initials: H
authors.lastname: Li
authors.forename: Peter C
authors.initials: PC
authors.lastname: Chien
authors.forename: Michael
authors.initials: M
authors.lastname: Tuen
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Visciano
authors.forename: Sandra
authors.initials: S
authors.lastname: Cohen
authors.forename: Steven
authors.initials: S
authors.lastname: Blais
authors.forename: Chong-Feng
authors.initials: CF
authors.lastname: Xu
authors.forename: Hui-Tang
authors.initials: HT
authors.lastname: Zhang
authors.forename: Catarina E
authors.initials: CE
authors.lastname: Hioe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D017951:Antigen Presentation / Q000276:immunology*; D001666:Binding Sites, Antibody; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D018984:Epitopes, T-Lymphocyte / Q000276:immunology / Q000378:metabolism*; D006031:Glycosylation; D015699:HIV Envelope Protein gp120 / Q000276:immunology / Q000378:metabolism*; D015497:HIV-1 / Q000276:immunology / Q000378:metabolism*; D006681:HLA-D Antigens / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D018984:Epitopes, T-Lymphocyte; D015699:HIV Envelope Protein gp120; D006681:HLA-D Antigens; D011134:Polysaccharides; C514793:gp120 protein, Human immunodeficiency virus 1