title: [To explore the clinical significance of HBV large protein (HBV-LP) in diagnosing viral replication].
abstract: OBJECTIVE
  To explore the clinical significance of HBV large protein (HBV-LP) in diagnosing viral replication, we detected the HBV-LP, HBV DNA and the hepatitis B viral markers (HBV M) in the serum of the patients infected with HBV.
  
  
  METHODS
  HBV-LP and HBV M were analyzed by using ELISA. HBV-DNA was quantitatively detected using real-time fluorescent polymerase chain reaction.
  
  
  RESULTS
  (1) No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the same HBV M (P 0.05). (2) No significant difference between the positive rate of HBV DNA and HBV-LP was found in HBeAg negative patients. (3) The contents of serum HBV-LP was positively correlated with the number of HBV DNA copies.
  
  
  CONCLUSIONS
  There was a close correlation between the positive rate of HBV-LP and HBV DNA, and HBV-LP is a reliable serological marker that can reflect the replication of HBV.
authors.affiliation: Department of Clinical, ZhenJiang Enter-Exceed territory Inspection Quarantine Bureau ZhenJiang 212008, China. gyh5511@163.com
authors.forename: Yu-hua
authors.initials: YH
authors.lastname: Gong
authors.forename: Yun
authors.initials: Y
authors.lastname: Hu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhang
authors.forename: Qin
authors.initials: Q
authors.lastname: Su
authors.forename: Ren-lian
authors.initials: RL
authors.lastname: Diao
authors.forename: Jia-wen
authors.initials: JW
authors.lastname: Miao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000097:blood; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006513:Hepatitis B e Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens