Evaluation of recA sequencing for the classification of Aeromonas strains at the genotype level.
OBJECTIVE
To evaluate the usefulness of partial recA sequences for the identification of Aeromonas strains at the genotype level.


RESULTS
A partial recA sequence was obtained from 21 type or reference strains and 33 Aeromonas isolates, collected in the South of Switzerland from human, animal and aquatic environments. The 272 bp long recA fragments showed a mean interspecies divergence of 7.8% and allowed the classification of strains at genotype level. However, some discrepancies could be observed with other gene sequence based analyses in the classification of some strains.


CONCLUSIONS
The 272 bp long recA fragment is a good molecular marker to infer taxonomy of members of the genus Aeromonas, even if the primers we chose for the amplification did not allow its direct sequencing.


CONCLUSIONS
In the genus Aeromonas, nucleotide sequences of some protein-encoding genes have already been evaluated as molecular markers to be used in taxonomical and epidemiological researches. This study suggests the usefulness of a recA fragment as a further sequence to investigate for these purposes.
Sepe
A
A
Laboratory of Environmental Microbiology, Istituto Cantonale di Microbiologia, Bellinzona, Switzerland, and Università degli Studi dell'Insubria, Varese, Italy.
Barbieri
P
P
Peduzzi
R
R
Demarta
A
A
D000333:Aeromonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002417:Cattle; D002645:Chickens; D002648:Child; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D004783:Environmental Microbiology; D006041:Goats; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000662:veterinary*; D006736:Horses; D006801:Humans; D010802:Phylogeny; D011938:Rec A Recombinases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013557:Switzerland; D014426:Turtles
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011938:Rec A Recombinases