Rapid purification of annexin V from human placenta by affinity chromatography.
Affinity purification of annexin V from human placenta on column with appropriate monospecific antibodies is developed. The procedure permits purification of the protein to a highly purified state by a two stage procedure. The yield of the protein is about 5 mg per 100 g of wet tissue. Because of high homologies between various annexins, it was supposed that this procedure can be also applied for purification of other annexins from other tissues.
Mikaelyan
M V
MV
Institute of Biochemistry, National Academy of Sciences, Yerevan, Armenia.
Poghosyan
G G
GG
Gasparyan
V K
VK
D017304:Annexin A5 / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D010920:Placenta / Q000378:metabolism*
D017304:Annexin A5