title: Isolation of carrot Argonaute1 from subtractive somatic embryogenesis cDNA library.
abstract: Carrot Argonaute1 (C-Ago1) was isolated from a subtractive cDNA library to obtain somatic embryogenesis related genes. C-Ago1 has three conserved domains, which are found in all other Argonautes. C-Ago1 has specific expression during somatic embryogenesis, which indicates that microRNA gene expression controlling system is required for somatic embryogenesis.
authors.affiliation: Department of Plant Gene and Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Sakyo, Kyoto 606-8502, Japan. kimi2takahata@hotmail.com
authors.forename: Kiminori
authors.initials: K
authors.lastname: Takahata
mesh_terms: D018552:Daucus carota / Q000737:chemistry* / Q000196:embryology*; D047108:Embryonic Development / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D035683:MicroRNAs / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000166:cytology
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins