Efficient immobilization and patterning of live bacterial cells.
A monolayer of live bacterial cells has been patterned onto substrates through the interaction between CFA/I fimbriae and the corresponding antibody. Patterns of live bacteria have been prepared with cellular resolution on silicon and gold substrates for Salmonella enterica serovar Typhimurium as a model with high specificity and efficiency. The immobilized cells are capable of dividing in growth medium to form a self-sustaining bacterial monolayer on the patterned areas. Interestingly, the immobilized cells can alter their orientation on the substrate, from lying-down to standing-up, as a response to the cell density increase during incubation. This method was successfully used to sort a targeted bacterial species from a mixed culture within 2 h.
Suo
Zhiyong
Z
Imaging and Chemical Analysis Laboratory, Department of Physics, and Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717, USA.
Avci
Recep
R
Yang
Xinghong
X
Pascual
David W
DW
D018914:Cells, Immobilized; D004926:Escherichia coli / Q000737:chemistry* / Q000166:cytology*; D050296:Microbial Viability; D018625:Microscopy, Atomic Force; D012486:Salmonella typhimurium / Q000737:chemistry* / Q000166:cytology*