title: Screening of senescence-associated genes with specific DNA array reveals the role of IGFBP-3 in premature senescence of human diploid fibroblasts.
abstract: Repeated exposures to sublethal concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of WI-38 human diploid fibroblasts. We found 16 replicative senescence-related genes with similar alterations in expression level in replicative senescence and two models of stress-induced premature senescence. Among these genes was IGFBP-3. Using a siRNA approach, we showed that IGFBP-3 regulates the appearance of several biomarkers of senescence after repeated exposures of WI-38 fibroblasts to tert-butylhydroperoxide and ethanol.
authors.affiliation: Unit of Research on Cellular Biology, Department of Biology, University of Namur, Rue de Bruxelles, B-5000 Namur, Belgium.
authors.forename: Florence
authors.initials: F
authors.lastname: Debacq-Chainiaux
authors.forename: Thierry
authors.initials: T
authors.lastname: Pascal
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Boilan
authors.forename: Coralie
authors.initials: C
authors.lastname: Bastin
authors.forename: Emilie
authors.initials: E
authors.lastname: Bauwens
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
mesh_terms: D002460:Cell Line; D016922:Cellular Senescence; D004171:Diploidy; D000431:Ethanol / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D020869:Gene Expression Profiling; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D000431:Ethanol; D020122:tert-Butylhydroperoxide