title: Proteomic analysis of microsomes from lactating bovine mammary gland.
abstract: Mammary gland has multiple metabolic potential including for large-scale synthesis of milk proteins, carbohydrate, and lipids including nutrient triacylglycerols. We have carried out a proteomic analysis of mammary tissue to discover proteins that affect lipid metabolism. Unfractionated microsomes from lactating bovine mammary tissue were analyzed using one-dimensional SDS-PAGE with RPLC-ESI-MS/MS. This approach gave 703 proteins including 160 predicted transmembrane proteins. Proteins were classified according to their subcellular localizations and biological functions. Over 50 proteins were associated with cellular uptake, metabolism, and secretion of lipids, including some enzymes that have been previously associated with breast cancer and potential therapeutic targets. This database develops a proteomic view of the metabolic potential of mammary gland that can be expected to contribute to a greater understanding of gene expression and tissue remodeling associated with lactation, and to further dissection of normal and pathological processes in mammary tissue.
authors.affiliation: Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Peng
authors.forename: Pisana
authors.initials: P
authors.lastname: Rawson
authors.forename: Danyl
authors.initials: D
authors.lastname: McLauchlan
authors.forename: Klaus
authors.initials: K
authors.lastname: Lehnert
authors.forename: Russell
authors.initials: R
authors.lastname: Snell
authors.forename: T William
authors.initials: TW
authors.lastname: Jordan
mesh_terms: D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000032:analysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007774:Lactation / Q000378:metabolism*; D050356:Lipid Metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002352:Carrier Proteins; D020543:Proteome