Synthesis and characterization of core-shell star copolymers for in vivo PET imaging applications.
The synthesis of core-shell star copolymers via living free radical polymerization provides a convenient route to three-dimensional nanostructures having a poly(ethylene glycol) outer shell, a hydrophilic inner shell bearing reactive functional groups, and a central hydrophobic core. By starting with well-defined linear diblock copolymers, the thickness of each layer, overall size/molecular weight, and the number of internal reactive functional groups can be controlled accurately, permitting detailed structure/performance information to be obtained. Functionalization of these polymeric nanoparticles with a DOTA-ligand capable of chelating radioactive (64)Cu nuclei enabled the biodistribution and in vivo positron emission tomography (PET) imaging of these materials to be studied and correlated directly to the initial structure. Results indicate that nanoparticles with increasing PEG shell thickness show increased blood circulation and low accumulation in excretory organs, suggesting application as in vivo carriers for imaging, targeting, and therapeutic groups.
Fukukawa
Ken-ichi
K
Materials Research Laboratory, Department of Chemistry, University of California, Santa Barbara, California 93106, USA.
Rossin
Raffaella
R
Hagooly
Aviv
A
Pressly
Eric D
ED
Hunt
Jasmine N
JN
Messmore
Benjamin W
BW
Wooley
Karen L
KL
Welch
Michael J
MJ
Hawker
Craig J
CJ
D000818:Animals; D003301:Copper Radioisotopes; D004337:Drug Carriers; D005260:Female; D006573:Heterocyclic Compounds, 1-Ring / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D053758:Nanoparticles; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D014018:Tissue Distribution
D003301:Copper Radioisotopes; D004337:Drug Carriers; D006573:Heterocyclic Compounds, 1-Ring; D011108:Polymers; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; D011092:Polyethylene Glycols