title: Proteome analysis of aflatoxin B1-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) and functional identification of candidate protein peroxiredoxin II.
abstract: In order to explore the proteins responsible for hepatocellular carcinoma (HCC), aflatoxin B(1)-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) was analyzed with 2-DE and MS. By comparing HCC samples with their own precancerous biopsies and HCC-surrounding tissues, a group of candidate proteins that differentially expressed in HCC were obtained. Peroxiredoxin (Prx) II, one of the candidates with distinct alteration, was further investigated and validated. Western blot and RT-PCR assays confirmed the overexpression of Prx II in both tree shrew and human HCC tissues. RNA interference for silencing Prx II was employed subsequently to explore the function and underlying mechanism of Prx II on liver cancer cell line Hep3B. Results showed the cell proliferation and clone formation decreased obviously when Prx II expression was inhibited, while the flow cytometer analysis showed the percentage of cell apoptosis enhanced. Inhibition of Prx II expression also obviously increased the generation of ROS and malondialdehyde, both are the products from peroxidation. These results imply the important role of Prx II in hepatocarcinogenesis, possibly through its function in regulating peroxidation and hereby to provide a favorable microenvironment for cancer cell surviving and progressing.
authors.affiliation: Department of Experimental Pathology, Guangxi Cancer Institute, Nanning, China. liyuangx@yahoo.com.cn
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Xue
authors.initials: X
authors.lastname: Qin
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Cui
authors.forename: Zhi
authors.initials: Z
authors.lastname: Dai
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Kang
authors.forename: Haiying
authors.initials: H
authors.lastname: Yue
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianjia
authors.initials: J
authors.lastname: Su
authors.forename: Ji
authors.initials: J
authors.lastname: Cao
authors.forename: Chao
authors.initials: C
authors.lastname: Ou
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Duan
authors.forename: Huifen
authors.initials: H
authors.lastname: Yue
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000328:Adult; D016604:Aflatoxin B1; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D054464:Peroxiredoxins / Q000096:biosynthesis / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014417:Tupaia
chemical_list: D016604:Aflatoxin B1; D054464:Peroxiredoxins