[IFN-gamma promotes human monocytes to differentiate to untypical mature DC].
OBJECTIVE
To explore the morphologic and phenotypic changes of monocytess in response to the stimulation of IFN-gamma, TNF-alpha and IFN-alpha.


METHODS
Newly isolated monocytes from human PBMCs were treated with IFN-gamma, TNF-alpha or IFN-alpha. The phenotypic growth of monocytes with or without stimulation was observed by microscope. The surface expression of CD1a, CD14, CD80, CD83, CD86, and HLA-DR on the monocytes was assayed by flow cytometry.


RESULTS
After IFN-gamma stimulation, monocytes displayed the shape of fusiform or polygon and were more likely to adhere and cluster to form the distinct structure as "cell islet". IFN-gamma; also induced or up-regulated the surface expression of CD80, CD83, CD86, and HLA-DR on the monocytes and down-regulated the CD14 expression. IFN-gamma even induced the CD1a expression and changed the monocytes phenotype from CD14(+)CD1a(-)CD83(-) to CD14(+)CD1a(+)CD83(+) after stimulated for 5 days. Cytokines TNF-alpha and IFN-alpha; have the different impacts on monocytes from IFN-gamma.


CONCLUSIONS
IFN-gamma have the immunoregulatory function of making monocytes differentiate to untypical mature dendritic cells (DC).
Wang
Hui-ming
HM
Renal Department of Daping Hospital Chongqing 400042, China. hm_wang72@163.com
Wang
Yi-qin
YQ
Ruan
Zhi-hua
ZH
Fu
Xiao-lan
XL
Xu
Wen-yue
WY
Zhao
Ting-ting
TT
Wu
Yu-zhang
YZ
D015703:Antigens, CD / Q000378:metabolism; D018949:Antigens, CD1 / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D000097802:CD83 Antigen
D015703:Antigens, CD; D018949:Antigens, CD1; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D007136:Immunoglobulins; D016898:Interferon-alpha; D007371:Interferon-gamma; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D014409:Tumor Necrosis Factor-alpha; D000097802:CD83 Antigen; C419393:CD1a antigen