title: [Study on effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats].
abstract: OBJECTIVE
  To build an experimental method to determine the content of puerarin in plasma, observe the effect of different combinations of traditional Chinese medicine preparation active parts on metabolism of puerarin in plasma of rats suffering cerebral ischemia attack after being orally administered the main active parts of Yangyin Tongnao granules.
  
  
  METHODS
  Absorption of puerarin in plasma was extracted by acetonitrile. Using hydroxybenzoate as interior standard, methanol-0.5% acetic acid water solution as mobile phase, ODS C(18) chromatography column (4.6 mm x 150 mm, 5 microm) as stationary phase, puerarin was detected at 248 nm.
  
  
  RESULTS
  There is a good linear relationship for puerarin in the range 0.025-3.2 microg x mL-1, RSD < 10%, and the average recovery is 97.6%.
  
  
  CONCLUSIONS
  The method is sensitive, reliable, and can be well applied in this experiment. It can provide a method for determination of active components in TCM preparation in vitro. Different compatibility influences metabolism of components.
authors.affiliation: Institute of Heart Brain Vessel Disease, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Hai-Tong
authors.initials: HT
authors.lastname: Wan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Yuan-Jiang
authors.initials: YJ
authors.lastname: Pan
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D019540:Area Under Curve; D002545:Brain Ischemia / Q000097:blood; D004338:Drug Combinations; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification; D007529:Isoflavones / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000470:Alkaloids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D047309:Flavones; D007529:Isoflavones; D009822:Oils, Volatile; D012503:Saponins; C033607:puerarin