title: An easier method for locating replication origins based on real-time polymerase chain reaction.
abstract: Locating replication origins (ROs) is an old problem to which diverse answers have been previously given. However, a better and more convenient method still remains desirable because previously reported methods are time-consuming. In this study, we report an easier method for locating ROs based on real-time polymerase chain reaction. The feasibility of this method was demonstrated using Escherichia coli K-12 and Halobacterium salinarium NRC-1 as models. Our results show that this method is easy, fast, and accurate in locating ROs.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Mei
authors.forename: D
authors.initials: D
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: P
authors.initials: P
authors.lastname: Shen
authors.forename: X
authors.initials: X
authors.lastname: Chen
mesh_terms: D017931:DNA Primers / Q000032:analysis / Q000235:genetics; D019641:DNA, Archaeal / Q000032:analysis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D048168:Escherichia coli K12 / Q000235:genetics*; D019617:Halobacterium salinarum / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018741:Replication Origin; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D019641:DNA, Archaeal; D004269:DNA, Bacterial