Scanning conductance microscopy investigations on fixed human chromosomes.
Scanning conductance microscopy investigations were carried out in air on human chromosomes fixed on pre-fabricated SiO2 surfaces with a backgate. The point of the investigation was to estimate the dielectric constant of fixed human chromosomes in order to use it for microfluidic device optimization. The phase shift caused by the electrostatic forces, together with geometrical measurements of the atomic force microscopy (AFM) cantilever and the chromosomes were used to estimate a value for the dielectric constant of different human chromosomes.
Clausen
Casper Hyttel
CH
MIC-Department of Micro and Nanotechnology, Technical University of Denmark, Lyngby, Denmark. chc@mic.dtu.dk
Lange
Jacob Moresco
JM
Jensen
Linda Boye
LB
Shah
Pranjul Jaykumar
PJ
Dimaki
Maria Ioannou
MI
Svendsen
Winnie Edith
WE
D002877:Chromosomes, Human / Q000737:chemistry*; D006801:Humans; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*