Highly selective and rapid arsenic removal by metabolically engineered Escherichia coli cells expressing Fucus vesiculosus metallothionein.
An arsenic-chelating metallothionein (fMT) from the arsenic-tolerant marine alga Fucus vesiculosus was expressed in Escherichia coli, resulting in 30- and 26-fold-higher As(III) and As(V) binding, respectively. Coexpression of the As(III)-specific transporter GlpF with fMT further improved arsenic accumulation and offered high selectivity toward As. Resting E. coli cells coexpressing fMT and GlpF completely removed trace amounts (35 ppb) of As(III) within 20 min, providing a promising technology for compliance with the As limit of 10 ppb newly recommended by the U.S. EPA.
Singh
Shailendra
S
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521. wilfred@engr.ucr.edu.
Mulchandani
Ashok
A
Chen
Wilfred
W
D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D001151:Arsenic / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D044645:Fucus / Q000235:genetics; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D020346:Aquaporins; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; C078043:GlpF protein, E coli; D008668:Metallothionein; D001151:Arsenic