Preventive effects of genistein on leukocyte adhesion in femur venules and on bone-loss induced in ovariectomized female rats.
This study was aimed to examine effects of genistein on leukocyte adhesion in femur microcirculation in relation to bone-loss induced in ovariectomized female rats. Sixty-four female Wistar rats were divided into 4 groups: sham (daily treated with vehicle; DMSO, sc; 100 microl/day), ovariectomized rat treated with vehicle (OVX(veh)), 17beta-estradiol treated-ovariectomized rat (OVX(E2), 5 microg/kg/day, s.c.) and genistein treated-ovariectomized rat (0.25 mg/kg/day, s.c.; OVX(gen)). One and three weeks after the ovariectomy, blood flow perfusion (BF) in femur tissue was measured using laser Doppler flowmetry. Leukocyte adhesion in femur venules (15-30 microm in diameter) of each group was evaluated by intravital fluorescence videomicroscopy. The bone mineral content (BMC) was measured and expressed in terms of ratio of ash-to-dry matter weight. Serum osteocalcin and alkaline phosphatase levels were determined using chemiluminescence immunoassay. In both one and three week-OVX(veh), leukocyte adhesion increased significantly, compared to their age-matched sham groups, but it decreased significantly in OVX(gen), compared to OVX(veh) (p<0.05). In three week-OVX(gen), both BF and BMC increased significantly, but osteocalcin and alkaline phosphatase levels decreased, compared to those of three week-OVX(veh). In conclusion, genistein supplementation could effectively prevent bone-loss and microvascular endothelial dysfunction induced by estrogen deficiency.
Kasiyaphat
Atchareeya
A
Department of Sport Science, Faculty of Physical Education, Srinakharinwirot University, Bangkok 10110, Thailand.
Siriviriyakul
Prasong
P
Patumraj
Suthiluk
S
D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000098:blood supply*; D002448:Cell Adhesion / Q000187:drug effects*; D004195:Disease Models, Animal; D005260:Female; D005269:Femur / Q000098:blood supply / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects; D018715:Microscopy, Video; D010052:Ovariectomy; D048789:Phytoestrogens / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014699:Venules / Q000187:drug effects*
D048789:Phytoestrogens; D019833:Genistein