Novel approach to fast determination of multiple pesticide residues using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
A rapid, high-throughput method employing ultra-performance liquid chromatography with tandem quadrupole mass spectrometry (UPLC-MS/MS) was developed and optimized for simultaneous quantification and confirmation of 64 pesticide residues and their toxic metabolites in fruit extracts prepared by a buffered QuEChERS procedure. The total time required for UPLC-MS/MS analysis was 8 min plus 2 min for re-equilibration to the initial UPLC conditions. Performance characteristics were determined for apple extracts spiked at 10 microg kg(-1). The repeatability of measurements expressed as relative standard deviations was in the range 1.5-13% at this level for most analytes. Thanks to very low limits of quantification (<10 microg kg(-1)for the majority of pesticides), an optimized method allows for the reliable control of not only common maximum residue limits (MRLs) set by European Union regulation for various pesticides/fruit combinations, but also of a uniform MRL of 10 microg kg(-1)endorsed for baby food.
Kovalczuk
T
T
Department of Food Chemistry and Analysis, Institute of Chemical Technology, Technická 3, CZ-166 28 Prague 6, Czech Republic.
Lacina
O
O
Jech
M
M
Poustka
J
J
Hajslová
J
J
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005062:European Union; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000592:standards; D027845:Malus / Q000737:chemistry*; D008452:Maximum Allowable Concentration; D010573:Pesticide Residues / Q000032:analysis*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
D010573:Pesticide Residues