title: [Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin].
abstract: OBJECTIVE
  To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC).
  
  
  METHODS
  The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate: n-butanol: water (2 : 1 : 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm.
  
  
  RESULTS
  136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes.
  
  
  CONCLUSIONS
  The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian 116027, China.
authors.forename: You-Wei
authors.initials: YW
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Xu
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Xu
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Peng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D030012:Gentiana / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012117:Resins, Synthetic; D027343:Rhizome / Q000737:chemistry
chemical_list: D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D012117:Resins, Synthetic; C012997:gentiopicroside