title: Evaluation of the Korean isolate-1 tachyzoite antigen for serodiagnosis of toxoplasmosis.
abstract: To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Republic of Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Shin
authors.forename: Dong-Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Aifen
authors.initials: A
authors.lastname: Lin
authors.forename: Jo-Woon-Yi
authors.initials: JW
authors.lastname: Lee
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Myoung-Hee
authors.initials: MH
authors.lastname: Ahn
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007723:Korea; D007841:Latex Fixation Tests; D008297:Male; D051379:Mice; D008875:Middle Aged; D011817:Rabbits; D012698:Serologic Tests; D014123:Toxoplasmosis / Q000097:blood / Q000175:diagnosis*
chemical_list: D000953:Antigens, Protozoan