Simultaneous determination of two Amadori compounds in Korean red ginseng (Panax ginseng) extracts and rat plasma by high-performance anion-exchange chromatography with pulsed amperometric detection.
A new simple, rapid and sensitive high-performance anion-exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) was developed and validated for the simultaneous determination of two Amadori compounds, arginyl-fructose and arginyl-fructosyl-glucose in Korean red ginseng (Panax ginseng) extracts, rat plasma. Separation of the two target analytes was efficiently undertaken on CarboPac PA1 anion-exchange column with isocratic elution (400 mM sodium hydroxide and deionized water (90:10, v/v)) at flow rate 0.7 mL/min within 15 min of single chromatographic run. Under optimized conditions, the detection limits (signal-to-noise ratio equal to 3) were 20 and 25 ng/mL for arginyl-fructose and arginyl-fructosyl-glucose, respectively. Calibration curves of peak area for the two analytes were linear over three orders of magnitude with a correlation coefficients greater than 0.999. The accuracy of the method was tested by recovery measurement of the spiked samples which yielded good results of 94.15-102.62%. This method was successfully applied to the quantification of arginyl-fructose and arginyl-fructosyl-glucose in herbal extracts and in the plasma samples from rat.
Joo
Kyung-Mi
KM
Skin Research Institute, Amorepacific Corporation R&D Center, 314-1 Bora-Dong, Giheung-Gu, Yongin-Si, Gyeonggi-Do 446-729, Republic of Korea. kmjoo@amorepacific.com
Park
Chan-Woong
CW
Jeong
Hye-Jin
HJ
Lee
Sang Jun
SJ
Chang
Ih Seop
IS
D000818:Animals; D000837:Anion Exchange Resins; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D005894:Panax / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000837:Anion Exchange Resins; D010940:Plant Proteins