title: Quantitative detection of metallo-beta-lactamase of blaIMP-cluster-producing Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis for rapid diagnosis and treatment of nosocomial infection.
abstract: In this study, we established the rapid quantitative detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa in clinical isolates and samples using real-time polymerase chain reaction (PCR) targeting gyrB (identification of P. aeruginosa) and blaIMP. The relative sensitivities and specificities of this real-time PCR assay were as follows: 100.0% and 100.0% for clinical isolates, and 100.0% and 98.4% for clinical specimens, respectively. The relative sensitivities and specificities of blaIMP-PCR were 100.0% in both clinical isolates and clinical specimens. The present PCR assay was easily and quickly performed, and it accurately detected P. aeruginosa and metallo-beta-lactamase.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki City, Nagasaki, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Motoshima
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Morinaga
authors.forename: Junichi
authors.initials: J
authors.lastname: Matsuda
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sugahara
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hirakata
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Yamada
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
authors.forename: Shimeru
authors.initials: S
authors.lastname: Kamihira
mesh_terms: D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D027081:DNA Gyrase / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011552:Pseudomonas Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000627:therapeutic use
chemical_list: D047090:beta-Lactams; D001618:beta-Lactamases; D027081:DNA Gyrase