title: Dramatic improvement in FMD DNA vaccine efficacy and cross-serotype antibody induction in pigs following a protein boost.
abstract: To overcome the low and slow development of humoral antibody often observed with DNA vaccines we applied a prime-boost strategy. When FMD DNA vaccine P1-2A3C3D and pGM-CSF primed pigs were boosted with inactivated foot-and-mouth disease virus (FMDV) antigen and recombinant 3D (without adjuvant) an average 36-fold increase in the FMDV antibody response was observed compared to conventional vaccination, that included a log(10) virus neutralising titre increase. Most remarkably, a significant level of cross-serotype reactivity was observed against A, C and Asia1 in the virus neutralisation and ELISA tests. This prime-boost strategy fully protected pigs from a heterologous challenge.
authors.affiliation: Institute for Animal Health Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, UK. yanmin.li@bbsrc.ac.uk
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Li
authors.forename: Catrina M A
authors.initials: CM
authors.lastname: Stirling
authors.forename: Michael S
authors.initials: MS
authors.lastname: Denyer
authors.forename: Pippa
authors.initials: P
authors.lastname: Hamblin
authors.forename: G
authors.initials: G
authors.lastname: Hutchings
authors.forename: Haru-H
authors.initials: HH
authors.lastname: Takamatsu
authors.forename: Paul V
authors.initials: PV
authors.lastname: Barnett
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology; D003429:Cross Reactions; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D007117:Immunization, Secondary / Q000379:methods*; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D013552:Swine; D019444:Vaccines, DNA / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D014765:Viral Vaccines; C103557:virus-infection-associated antigen, Foot-and-mouth disease virus