title: [Microarray-based methods to identify DNA methylation in cancer].
abstract: DNA methylation is an epigenetic modification associated with gene transcription regulation, X-chromosome inactivation, regulation of development and cell differentiation. Aberrant DNA methylation has been linked to cancer. The advent of microarray technology has provided new opportunities for high-throughput study on DNA methylation. Various microarray-based methods depend on different pretreatments including immunoprecipitation and restriction digestion. Al-though each technique has its limitations, the immunoprecipitation method has high specificity, and the restriction digestion method shows high sensitivity. Together they provide many choices to the study of genome-wide DNA methylation profile in cancer.
authors.affiliation: Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases, Shanghai 200001, China. txqwl2004@yahoo.com.cn
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Tian
authors.forename: Dan-Feng
authors.initials: DF
authors.lastname: Sun
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Fang
mesh_terms: D000818:Animals; D019175:DNA Methylation / Q000235:genetics*; D006801:Humans; D008957:Models, Genetic; D009369:Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*