Protective role of new nitrogen compounds on ROS/RNS-mediated damage to PC12 cells.
Reactive oxygen (ROS) and nitrogen (RNS) species are known to be involved in many degenerative diseases. This study reports four new nitrogen compounds from organic synthesis, identified as FMA4, FMA7, FMA762 and FMA796, which differ mainly by the number of hydroxyl groups within their phenolic unit. Their potential role as antioxidants was evaluated in PC12 cells by assessing their protection against oxidative and nitrosative insults. The four compounds, and particularly FMA762 and FMA796, were able to protect cells against lipid peroxidation and intracellular ROS/RNS formation to a great extent. Their protective effects were likely mediated by their free radicals scavenging ability, as they appeared to be involved neither in the induction of natural antioxidant enzymes like GSH-PX and SOD, nor in the inhibition of NOS. Nevertheless, these results suggest a promising potential for these compounds as ROS/RNS scavengers in pathologies where oxidative/nitrosative stress are involved.
Silva
João P
JP
CBMA - Molecular and Environmental Biology Centre/Biology Department, University of Minho, Campus de Gualtar, Braga, Portugal.
Proença
Fernanda
F
Coutinho
Olga P
OP
D000578:Amidines / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001713:Biphenyl Compounds; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D015227:Lipid Peroxidation / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000633:toxicity; D009599:Nitroprusside / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010851:Picrates / Q000737:chemistry; D051381:Rats; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism
D000578:Amidines; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D020030:Nitric Oxide Donors; D010851:Picrates; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D009599:Nitroprusside; C004931:1,1-diphenyl-2-picrylhydrazyl; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione