title: Comparison of E-Test, disk diffusion and a modified CLSI broth microdilution (M 38-A) method for in vitro testing of itraconazole, fluconazole and voriconazole against dermatophytes.
abstract: We compared two agar-based methods, the E-test and the disk diffusion method with the Clinical Laboratory Standards Institute (CLSI) reference broth microdilution method (CLSI M38-A; MD). Forty six dermatophytes strains including 30 Trichophyton mentagrophytes, 8 T. rubrum and 8 M. gypseum were tested against three antifungal agents, i.e., fluconazole (FLC), itraconazole (ITC) and voriconazole (VRC). The level of agreement between the E-test and MD (+/-2 dilutions) was 45.6% for fluconazole, 19.5% for itraconazole and 52.1% for voriconazole. The results obtained with disk diffusion had low correlation with the results obtained by the CLSI broth microdilution reference method with azoles.
authors.affiliation: Servicio de Microbiología Clínica y Parasitología Clínica, Hospital Universitario de Valme, Cádiz, Ctra, Spain.
authors.forename: Carmen Castro
authors.initials: CC
authors.lastname: Méndez
authors.forename: Maria Carmen
authors.initials: MC
authors.lastname: Serrano
authors.forename: Anastasio
authors.initials: A
authors.lastname: Valverde
authors.forename: Javier
authors.initials: J
authors.lastname: Pemán
authors.forename: Carmen
authors.initials: C
authors.lastname: Almeida
authors.forename: Estrella
authors.initials: E
authors.lastname: Martín-Mazuelos
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects* / Q000254:growth & development; D001230:Aspergillus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D052978:Disk Diffusion Antimicrobial Tests; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011743:Pyrimidines / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; D017964:Itraconazole; D000666:Amphotericin B; D065819:Voriconazole