title: Two cDNA clones (FsDhn1 and FsClo1) up-regulated by ABA are involved in drought responses in Fagus sylvatica L. seeds.
abstract: Two abscisic acid (ABA)-responsive clones (FsDhn1 and FsClo1) were isolated from a cDNA library of ABA-treated seeds of Fagus sylvatica L. FsDhn1 codes for type-II late embryogenesis abundant (LEA) proteins, also known as dehydrins. The corresponding transcripts were ABA-induced and expressed when seeds were artificially dried. FsClo1 codes for a caleosin, a calcium-binding protein. By expressing FsCLO1 as a fusion protein in Escherichia coli, we obtained biochemical evidence of its ability to bind calcium, a second messenger involved in ABA signaling. This cDNA clone is seed specific, shows a LEA expression pattern and is also induced by ABA and dehydration treatments. The relationship of both genes with seed responses to ABA is discussed.
authors.affiliation: Departamento de Fisiología Vegetal, Centro Hispano-Luso de Investigaciones Agrarias, Facultad de Biología, Universidad de Salamanca, Plaza de los Doctores de la Reina s/n, 37007 Salamanca, Spain.
authors.forename: Jesús Angel
authors.initials: JA
authors.lastname: Jiménez
authors.forename: Ana
authors.initials: A
authors.lastname: Alonso-Ramírez
authors.forename: Carlos
authors.initials: C
authors.lastname: Nicolás
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002999:Clone Cells; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D055864:Droughts; D047108:Embryonic Development / Q000187:drug effects; D029964:Fagus / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects*; D014867:Water / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; C066246:late embryogenesis abundant protein, plant; D014867:Water; D000040:Abscisic Acid; D002118:Calcium