Proteome analysis of aflatoxin B1-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) and functional identification of candidate protein peroxiredoxin II.
In order to explore the proteins responsible for hepatocellular carcinoma (HCC), aflatoxin B(1)-induced hepatocarcinogenesis in tree shrew (Tupaia belangeri chinensis) was analyzed with 2-DE and MS. By comparing HCC samples with their own precancerous biopsies and HCC-surrounding tissues, a group of candidate proteins that differentially expressed in HCC were obtained. Peroxiredoxin (Prx) II, one of the candidates with distinct alteration, was further investigated and validated. Western blot and RT-PCR assays confirmed the overexpression of Prx II in both tree shrew and human HCC tissues. RNA interference for silencing Prx II was employed subsequently to explore the function and underlying mechanism of Prx II on liver cancer cell line Hep3B. Results showed the cell proliferation and clone formation decreased obviously when Prx II expression was inhibited, while the flow cytometer analysis showed the percentage of cell apoptosis enhanced. Inhibition of Prx II expression also obviously increased the generation of ROS and malondialdehyde, both are the products from peroxidation. These results imply the important role of Prx II in hepatocarcinogenesis, possibly through its function in regulating peroxidation and hereby to provide a favorable microenvironment for cancer cell surviving and progressing.
Li
Yuan
Y
Department of Experimental Pathology, Guangxi Cancer Institute, Nanning, China. liyuangx@yahoo.com.cn
Qin
Xue
X
Cui
Jiefeng
J
Dai
Zhi
Z
Kang
Xiaonan
X
Yue
Haiying
H
Zhang
Yu
Y
Su
Jianjia
J
Cao
Ji
J
Ou
Chao
C
Yang
Chun
C
Duan
Xiaoxian
X
Yue
Huifen
H
Liu
Yinkun
Y
D000328:Adult; D016604:Aflatoxin B1; D000368:Aged; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D054464:Peroxiredoxins / Q000096:biosynthesis / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014417:Tupaia
D016604:Aflatoxin B1; D054464:Peroxiredoxins