title: Peripherally-derived BDNF promotes regeneration of ascending sensory neurons after spinal cord injury.
abstract: BACKGROUND
  The blood brain barrier (BBB) and truncated trkB receptor on astrocytes prevent the penetration of brain derived neurotrophic factor (BDNF) applied into the peripheral (PNS) and central nervous system (CNS) thus restrict its application in the treatment of nervous diseases. As BDNF is anterogradely transported by axons, we propose that peripherally derived and/or applied BDNF may act on the regeneration of central axons of ascending sensory neurons.
  
  
  RESULTS
  The present study aimed to test the hypothesis by using conditioning lesion of the sciatic nerve as a model to increase the expression of endogenous BDNF in sensory neurons and by injecting exogenous BDNF into the peripheral nerve or tissues. Here we showed that most of regenerating sensory neurons expressed BDNF and p-CREB but not p75NTR. Conditioning-lesion induced regeneration of ascending sensory neuron and the increase in the number of p-Erk positive and GAP-43 positive neurons was blocked by the injection of the BDNF antiserum in the periphery. Enhanced neurite outgrowth of dorsal root ganglia (DRG) neurons in vitro by conditioning lesion was also inhibited by the neutralization with the BDNF antiserum. The delivery of exogenous BDNF into the sciatic nerve or the footpad significantly increased the number of regenerating DRG neurons and regenerating sensory axons in the injured spinal cord. In a contusion injury model, an injection of BDNF into the footpad promoted recovery of motor functions.
  
  
  CONCLUSIONS
  Our data suggest that endogenous BDNF in DRG and spinal cord is required for the enhanced regeneration of ascending sensory neurons after conditioning lesion of sciatic nerve and peripherally applied BDNF may have therapeutic effects on the spinal cord injury.
authors.affiliation: Department of Human Physiology and Centre for Neuroscience, Flinders University, Adelaide, Australia.
authors.forename: Xing-Yun
authors.initials: XY
authors.lastname: Song
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Feng-He
authors.initials: FH
authors.lastname: Zhang
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Zhong
authors.forename: Xin-Fu
authors.initials: XF
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D001522:Behavior, Animal; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D019922:GAP-43 Protein / Q000378:metabolism; D005727:Ganglia, Spinal / Q000502:physiology*; D007124:Immunoenzyme Techniques; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins; D009475:Neurons, Afferent / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000503:physiopathology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D019922:GAP-43 Protein; D009419:Nerve Tissue Proteins; D017978:Receptors, Growth Factor; D017475:Receptors, Nerve Growth Factor; C068313:Ngfr protein, rat