title: Kid-mediated chromosome compaction ensures proper nuclear envelope formation.
abstract: Toward the end of mitosis, neighboring chromosomes gather closely to form a compact cluster. This is important for reassembling the nuclear envelope around the entire chromosome mass but not individual chromosomes. By analyzing mice and cultured cells lacking the expression of chromokinesin Kid/kinesin-10, we show that Kid localizes to the boundaries of anaphase and telophase chromosomes and contributes to the shortening of the anaphase chromosome mass along the spindle axis. Loss of Kid-mediated anaphase chromosome compaction often causes the formation of multinucleated cells, specifically at oocyte meiosis II and the first couple of mitoses leading to embryonic death. In contrast, neither male meiosis nor somatic mitosis after the morula-stage is affected by Kid deficiency. These data suggest that Kid-mediated anaphase/telophase chromosome compaction prevents formation of multinucleated cells. This protection is especially important during the very early stages of development, when the embryonic cells are rich in ooplasm.
authors.affiliation: Division of Oncology, Department of Cancer Biology, The University of Tokyo, Minato-ku, Tokyo, 108-8639, Japan.
authors.forename: Miho
authors.initials: M
authors.lastname: Ohsugi
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Adachi
authors.forename: Reiko
authors.initials: R
authors.lastname: Horai
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kakuta
authors.forename: Katsuko
authors.initials: K
authors.lastname: Sudo
authors.forename: Hayato
authors.initials: H
authors.lastname: Kotaki
authors.forename: Noriko
authors.initials: N
authors.lastname: Tokai-Nishizumi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sagara
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Iwakura
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000705:Anaphase; D000818:Animals; D001757:Blastomeres / Q000378:metabolism; D033481:Chromosomes, Mammalian / Q000378:metabolism*; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism*; D008297:Male; D051379:Mice; D009685:Nuclear Envelope / Q000378:metabolism*; D013692:Telophase
chemical_list: D004268:DNA-Binding Proteins; C098764:KIF22 protein, human; C527520:Kid protein, mouse; D016547:Kinesins