Colchicine against ischemia-reperfusion injury in experimental lung transplantation.
BACKGROUND
To asses the influence of colchicine, a potent antinflammatory agent and neutrophile migration inhibitor, on Ischemia-Reperfusion Injury (IRI) in rat lung isogeneic transplantation.


METHODS
Isogeneic, orthotropic single left lung transplantations were performed among male Wistar rats after total ischemic graft storage time of 12 or 18 hours in temperature of 4 degrees C. Recipients received either no specific treatment (control) or Colchicine 1.2 mg/kg/d ip. Twenty-four hours after transplantation, the native contralateral lung was occluded to assess graft gas exchange function (PaO(2)). The lung graft was excised and assessed for Wet/Dry ratio (W/D) as a measure of edema, Myeloperoxidase activity (MPO) as a measure of neutrophile migration and histology.


RESULTS
PaO(2) differences were not significant among all groups. Comparing colchicine to control group, the W/D ratio 3.93+/-0.66 vs. 1.86+/-0.32, p=0.002 and MPO 8.1+/-3.34 vs. 5.87+/-1.76, p=0.046 were significantly higher for 18 hours colchicine group. Comparing 18 to 12 hours time groups, the W/D ratio 5.70+/-1.53 vs. 1.86+/-0.32, p=0.007 for control groups and 5.40+/-1.49 vs. 3.93+/-0.66, p=0.049 for colchicine groups were significantly higher for both 12 h groups. Histology favored colchicine treated animals.


CONCLUSIONS
Colchicine in tested dose does not decrease edema after lung transplantation and does not improve lung function. 18 vs. 12 hours total ischemic graft storage time causes less lung edema.
Pieróg
Jaroslaw
J
Clinical Department of Thoracic Surgery, Pomeranian Medical University, Szczecin, Poland.
Kubisa
Bartosz
B
Grodzki
Tomasz
T
Wójcik
Janusz
J
Pankowski
Juliusz
J
Ostrowska
Justyna
J
Juzyszyn
Zygmunt
Z
Droździk
Marek
M
D000818:Animals; D003078:Colchicine / Q000627:therapeutic use*; D006084:Graft Rejection / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D011654:Pulmonary Edema / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D011659:Pulmonary Gas Exchange; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control*; D014185:Transplantation, Isogeneic; D050257:Tubulin Modulators / Q000627:therapeutic use*
D050257:Tubulin Modulators; D003078:Colchicine