A plate holder for non-destructive testing of mesophase crystallization assays.
Here, we describe a goniometer holder to mount standard 96-well crystallization plates directly onto the goniometer head of an oscillation camera. This attachment was designed to check crystallization conditions straight from the crystallization plates under X-rays, and was proven to be useful for checking small crystals and solutions that destabilize monoolein-based lipidic cubic phase (LCP) crystallization experiments. A quick procedure for setting up LCP assays employing commercially available instruments is also reported.
Agirre
J
J
Unidad de Biofísica (CSIC-UPV/EHU), Bilbao, Spain.
Mechaly
A
A
Cabo-Bilbao
A
A
Guérin
D M A
DM
D003460:Crystallization / Q000379:methods*; D003461:Crystallography / Q000379:methods*; D008422:Materials Testing / Q000379:methods*; D044367:Phase Transition; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D013048:Specimen Handling / Q000379:methods*; D014961:X-Ray Diffraction / Q000379:methods*
D011506:Proteins