title: Survival and recovery of apheresis platelets stored in a polyolefin container with high oxygen permeability.
abstract: OBJECTIVE
  Oxygen permeability is important in platelet storage media. We compared a new polyolefin container with enhanced oxygen permeability (PO-80; Kawasumi, Tokyo, Japan) to a widely used alternative (PL2410; Baxter Healthcare, Deerfield, IL, USA).
  
  
  METHODS
  In vitro characteristics of paired platelet concentrates (PCs; mean 4.2 x 10(11)/250 ml plasma/bag) stored in PO-80 or PL2410 were assessed through 9 days of storage. In vivo recovery and survival of 7-day-old autologous PCs were assessed according to the Murphy method.
  
  
  RESULTS
  Laboratory assessment of platelet quality favoured PO-80 during 9 days of storage with statistically significant differences in glucose consumption (2.75 vs. 4.93 mmol/10(12)/24 h in the interval 120-168 h), lactate generation (4.37 vs. 8.11 mmol/10(12)/24 h in the interval 120-168 h), pressure of oxygen (pO(2)) (59.3 vs. 38.1 mmHg at day 1), and HCO(3)(-) (14.7 vs. 13.4 mmol/l at day 1). Statistically significant differences were not seen in aggregation, hypotonic shock response or pH. In vivo assessment of autologous platelets stored 7 days in the PO-80 container revealed that recovery was 82.1% and survival was 81.0% of fresh control. Seven-day stored PCs in PO-80 were shown in vivo to be non-inferior to fresh platelets, with upper confidence limits (UCL(95)) in recovery and survival of stored PCs below the maximum acceptable difference (MAD); 15.3% UCL(95) < 20.4% MAD and 2.1 days UCL(95) < 2.1 days MAD.
  
  
  CONCLUSIONS
  The in vitro characteristics of PCs stored in a highly oxygen-permeable container were stable at least 7 days. The in vivo study supports the suitability of PO-80 for 7-day platelet storage.
authors.affiliation: Division of Blood Transfusion and Transplantation Immunology, Fukushima Medical University Hospital, Fukushima, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ezuki
authors.forename: T
authors.initials: T
authors.lastname: Kanno
authors.forename: H
authors.initials: H
authors.lastname: Ohto
authors.forename: L
authors.initials: L
authors.lastname: Herschel
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Kawabata
authors.forename: O
authors.initials: O
authors.lastname: Seino
authors.forename: K
authors.initials: K
authors.lastname: Ikeda
authors.forename: K E
authors.initials: KE
authors.lastname: Nollet
mesh_terms: D000094902:Blood Banking / Q000379:methods*; D001784:Blood Gas Analysis; D001792:Blood Platelets / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism; D010539:Permeability; D010969:Plastics / Q000737:chemistry / Q000494:pharmacology*; D017713:Platelet Transfusion; D010983:Plateletpheresis / Q000295:instrumentation*; D011090:Polyenes / Q000737:chemistry / Q000493:pharmacokinetics*; D013048:Specimen Handling
chemical_list: D010969:Plastics; D011090:Polyenes; C035051:PL 732; D010100:Oxygen