title: [Optimization of biotransformation conditions of active component in Panax notoginseng stalks and leaves by Fusarium sacchari].
abstract: By using Fusarium sacchari, a rare microbial strain isolated and screened from planted ginseng soil, the active component notoginseng triterpenes in Panax notoginseng stalks and leaves was biotransformed. Taking three main anti-tumor components, i. e., 20 (S)-protopanoxadiol-20-O-beta-D-glucopyranose (compound K, C-K), 20-O-beta-D-xylopyranosyl (1 --> 6) -beta-D-glucopyranosyl-20 (S)-protopanaxadiol (compound Mx, C-Mx) and 20 (S)-protopanoxadiol-20-O-alpha-L-arabofuranose (1 --> 6)-beta-D-glucopyranose (ginseng Mc, G-Mc) as evaluation indices, the optimization of biotransformation conditions of notoginseng triterpenes in P. notoginseng stalks and leaves were obtained by factor biotransformation experiment, i. e., initial pH value 6, substrate addition 40 mg, medium volume 30 ml, and transforming for 6 days at 30 degrees C. The method could increase the utility and economic benefit of P. notoginseng stalks and leaves effectively.
authors.affiliation: College of Resources and Civil Engineering, Northeastern University, Shenyang 110004, China. tase110@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Hu
authors.forename: Bin-Hui
authors.initials: BH
authors.lastname: Jiang
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000378:metabolism; D001711:Biotransformation; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D036145:Ginsenosides / Q000302:isolation & purification / Q000378:metabolism*; D005894:Panax / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D018547:Plant Stems / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012988:Soil Microbiology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D036145:Ginsenosides