Associations between gout tophus and polymorphisms 869T/C and -509C/T in transforming growth factor beta1 gene.
OBJECTIVE
To investigate the associations between gout tophus and polymorphisms 869T/C and -509C/T in TGF-beta1 gene.


METHODS
The polymorphisms 869T/C and -509C/T were determined in 73 gout patients and 114 healthy controls among male Taiwanese using the PCR-restriction fragment length polymorphism method. Each patient was matched with 1-2 controls by age within 1-2 yrs. The tophus number was measured from all the patients' arms and legs.


RESULTS
Neither 869T/C nor -509C/T showed a significant association between patients and controls in the proportions of genotypes, allele frequency or dominant and recessive models. The mean number of tophi for all patients was 1.53 +/- 3.44, showing a significant difference in distribution among the genotypes at polymorphism 869T/C (P = 0.006), but not those in polymorphism -509C/T (P > 0.05). Those carrying genotype CC at polymorphism 869T/C have a mean number of tophi 0.35 (+/- 1.11), which is significantly lower than those carrying genotype TT (3.73 +/- 4.67; P < 0.05). Those with genotype TT at polymorphism 869T/C also had 11.06 times the likelihood of having at least one tophus compared with the genotype CC after adjustment of hyperuricaemia (95% CI = 1.84, 66.36; P = 0.009). However, except for the tophus number, these two polymorphisms did not show any significant association with the clinical characteristics or biochemical markers.


CONCLUSIONS
The polymorphism 869T/C in TGF-beta1 gene has a significant association with the occurrence of tophus in gout patients.
Chang
S-J
SJ
Department of Public Health, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan. changsj@kmu.edu.tw
Chen
C-J
CJ
Tsai
F-C
FC
Lai
H-M
HM
Tsai
P-C
PC
Tsai
M-H
MH
Ko
Y-C
YC
D000328:Adult; D000704:Analysis of Variance; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006073:Gout / Q000473:pathology*; D006239:Haplotypes; D006801:Humans; D007596:Joints / Q000473:pathology*; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D053773:Transforming Growth Factor beta1