title: Increased carbonyl protein levels in the stratum corneum of the face during winter.
abstract: The stratum corneum (SC) is the interface between the body and the environment, and is continuously exposed to oxidative stress that results in carbonyl modification of proteins. We previously developed a simple and non-invasive method to assess the stratum corneum carbonyl protein (SCCP) levels. In this study, we used this method to examine the seasonal changes in the SCCP levels and the relationship between the SCCP level and the physiological condition of the SC. SC was collected from the face of healthy Japanese volunteers by adhesive tape stripping and its carbonyl groups were determined by reaction with fluorescein-5-thiosemicarbazide. The average fluorescence intensity of the SC was calculated as the SCCP level. The SCCP level in the cheek was higher in winter than summer. The SCCP level was negatively correlated with the water content in the SC measured by the conductance and capacitance, and also negatively correlated with the extensibility of the skin measured by a Cutometer, suggesting that the mechanical properties of the skin can be affected by oxidative modification of the SC. These data suggest the involvement of oxidative modification of SC proteins in the generation of rough skin during winter.
authors: Shiseido Life Science Research Center, 2-2-1 Hayabuchi, Tsuzuki-ku, Yokohama 224-8558, Japan.
authors: Y
authors: Y
authors: Kobayashi
authors: I
authors: I
authors: Iwai
authors: N
authors: N
authors: Akutsu
authors: T
authors: T
authors: Hirao
mesh_terms: D000293:Adolescent; D000328:Adult; D003080:Cold Temperature; D004817:Epidermis / Q000378:metabolism; D005145:Face; D005260:Female; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D050050:Protein Carbonylation; D011506:Proteins / Q000378:metabolism*; D012621:Seasons; D012867:Skin / Q000378:metabolism*
chemical_list: D005452:Fluoresceins; D011506:Proteins; C027912:fluorescein thiosemicarbazide