title: Modulation of the genomic estrogen receptor pathway by water extracts of Cirsium japonicum.
abstract: We investigated the estrogenic activity of Cirsium japonicum water extracts, which have long been used to treat vascular-related diseases. The activity of the extracts was characterized in a transient transfection system, using estrogen receptor isoforms and estrogen-responsive luciferase plasmids in HEK 293 cells. The extract activated both and estrogen receptors. Activation was inhibited by the estrogen receptor antagonist ICI 182,780, indicating that the effects were mediated through the estrogen receptor isoforms. Treatment with the extracts increased expression of the progesterone receptor and pS2 genes and expression of estrogen receptor was decreased in MCF-7 cells. These results suggested that the Cirsium japonicum water extracts showed estrogenic effects and may be a potential clinical application for treatment of estrogen related vascular diseases.
authors: College of Engineering, Institute of Biotechnology, Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea.
authors: Mi-Kyung
authors: MK
authors: Park
authors: Mee-ra
authors: MR
authors: Rhyu
authors: Byung-Koo
authors: BK
authors: Yoon
authors: Hyeok yi
authors: Hy
authors: Kwon
authors: Siyoul
authors: S
authors: Jang
authors: Young Joo
authors: YJ
authors: Lee
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D045744:Cell Line, Tumor; D031180:Cirsium / Q000737:chemistry*; D047628:Estrogen Receptor alpha / Q000187:drug effects / Q000235:genetics; D047629:Estrogen Receptor beta / Q000187:drug effects / Q000235:genetics; D006801:Humans; D007202:Indicators and Reagents; D008156:Luciferases / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects*; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014162:Transfection; D014867:Water
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D007202:Indicators and Reagents; D010936:Plant Extracts; D011960:Receptors, Estrogen; D012997:Solvents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014867:Water; D012313:RNA; D008156:Luciferases; C022616:thiazolyl blue