title: Spectrophotometric and reversed-phase high-performance liquid chromatographic methods for simultaneous determination of escitalopram oxalate and clonazepam in combined tablet dosage form.
abstract: Simple, accurate, precise, and sensitive ultraviolet spectrophotometric and reversed-phase high-performance liquid chromatographic (RP-HPLC) methods for simultaneous estimation of escitalopram oxalate (ESC) and clonazepam (CLO) in combined tablet dosage form have been developed and validated. The spectroscopic method employs an absorbance correction method using 238.6 and 308 nm as 2 wavelengths for estimation with methanol and water as solvents. Beer's law is obeyed in the concentration range of 10.0-50.0 and 0.5-3.0 micro/mL for ESC and CLO, respectively. The RP-HPLC method uses a Jasco HPLC system with HiQ SiL C18 column (250 x 4.6 mm id) acetonitrile-0.005 M tetrabutylammonium hydrogen sulfate (55 + 45, v/v) as the mobile phase, and satranidazole as an internal standard. The detection was carried out using an ultraviolet detector set at 287 nm. For the HPLC method, Beer's law is obeyed in the concentration range of 10.0-60.0 and 0.5-3.0 microg/mL for ESC and CLO, respectively. Both methods have been successfully applied for the analysis of the drugs in a pharmaceutical formulation. Results of analysis were validated statistically and by recovery studies.
authors: All India Shri Shivaji Memorial Society's (AISSMS) College of Pharmacy, Department of Pharmaceutical Analysis, Kennedy Rd, Near RTO, Pune, Maharashtra, India. santoshvgandhi@rediffmail.com
authors: Santosh Vilashchand
authors: SV
authors: Gandhi
authors: Nilesh Dnyandev
authors: ND
authors: Dhavale
authors: Vijay Yeshawantrao
authors: VY
authors: Jadhav
authors: Shweta Sadanand
authors: SS
authors: Sabnis
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015283:Citalopram / Q000032:analysis*; D002998:Clonazepam / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013607:Tablets
chemical_list: D013607:Tablets; D015283:Citalopram; D002998:Clonazepam