Begomoviruses: molecular cloning and identification of replication origin.
The Begomovirus genus is the largest genus of the Geminiviridae family and comprises the whitefly transmitted geminiviruses that infect dicotyledonous plants. They can be either mono or bipartite. In this chapter, we describe the cloning of begomovirus replication modules and the subsequent functional characterization of geminivirus replication origins.
Florentino
Lilian H
LH
Departamento de Bioquímica e Biologia Molecular, BIOAGRO-Universidade, Federal de Viçosa-36571.000, Viçosa, MG, Brazil.
Santos
Anésia A
AA
Zerbini
Francisco M
FM
Fontes
Elizabeth P B
EP
D001483:Base Sequence; D053839:Begomovirus / Q000235:genetics* / Q000472:pathogenicity; D003001:Cloning, Molecular / Q000379:methods; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification; D005822:Genetic Vectors; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010935:Plant Diseases / Q000821:virology; D018741:Replication Origin / Q000235:genetics*; D015183:Restriction Mapping
D004279:DNA, Viral