title: Nitrosative protein tyrosine modifications: biochemistry and functional significance.
abstract: Nitrosative modifications regulate cellular signal transduction and pathogenesis of inflammatory responses and neurodegenerative diseases. Protein tyrosine nitration is a biomarker of oxidative stress and also influences protein structure and function. Recent advances in mass spectrometry have made it possible to identify modified proteins and specific modified amino acid residues. For analysis of nitrated peptides with low yields or only a subset of peptides, affinity 'tags' can be bait for 'fishing out' target analytes from complex mixtures. These tagged peptides are then extracted to a solid phase, followed by mass analysis. In this review, we focus on protein tyrosine modifications caused by nitrosative stresses and proteomic methods for selective enrichment and identification of nitrosative protein modifications.
authors: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Korea.
authors: Woon-Seok
authors: WS
authors: Yeo
authors: Soo Jae
authors: SJ
authors: Lee
authors: Jung Rok
authors: JR
authors: Lee
authors: Kwang Pyo
authors: KP
authors: Kim
mesh_terms: D006801:Humans; D013058:Mass Spectrometry; D015538:Nitrosation; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D010455:Peptides; D011506:Proteins; D014443:Tyrosine