Effects of long-term exposure of extremely low frequency magnetic field on oxidative/nitrosative stress in rat liver.
Thirty-two adult Wistar-Albino female and male rats were used to investigate the long-term (45 days) effects of extremely low frequency magnetic field (ELF-MF; 50Hz, 1mT, 4h/day) exposure on oxidative/nitrosative stress in liver tissues of rats. The rats were divided randomly into four groups: female control (FC; n = 8) and MF-exposed female rats (F-MF; n = 8); male control (MC; n = 8) and MF-exposed male rats (M-MF; n = 8). Liver tissue from each animal was harvested and utilized for malondialdehyde (MDA) and 3-nitrotyrosine (3-NT) detection. MDA levels were measured by MDA-TBA method, while the 3-NT levels were determined by the HPLC-UV system. There were no significant differences between the MDA levels of the control (FC; MC) and MF-exposed (F-MF; M-MF) rats (P > 0.05). In the F-MF rats, 3-NT levels were significantly increased when compared to those of the FC rats (P < 0.05). There were no significant differences between the 3-NT levels of the MC and M-MF rats. In conclusion, our study suggests that the long-term ELF-MF exposure may enhance the oxidative/nitrosative stress in liver tissue of the female rats and could have a deteriorative effect on cellular proteins rather than lipids by enhancing 3-NT formation.
Erdal
Nurten
N
Department of Biophysics, Faculty of Medicine, Mersin University, Turkey. nerdal@mersin.edu.tr
Gürgül
Serkan
S
Tamer
Lülüfer
L
Ayaz
Lokman
L
D000818:Animals; D005260:Female; D008099:Liver / Q000502:physiology*; D008280:Magnetics; D008297:Male; D015538:Nitrosation; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000503:physiopathology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000096:biosynthesis
C002744:3-nitrotyrosine; D014443:Tyrosine