Rapid assay of trace immunoglobulin M by a new immunonanogold resonance scattering spectral probe.
Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH(2)PO( 4)-Na(2)HPO(4) buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm (DeltaI(580 nm)) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity.
Jiang
Zhiliang
Z
School of Environment and Resource, Guangxi Normal University, Guilin 541004, China. zljiang@mailbox.gxnu.edu.cn
Wei
Lili
L
Zou
Mingjing
M
Liang
Aihui
A
Meng
Mianwu
M
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007075:Immunoglobulin M / Q000097:blood*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry; D012542:Scattering, Radiation; D013057:Spectrum Analysis; D013997:Time Factors
D007075:Immunoglobulin M; D011092:Polyethylene Glycols; D006046:Gold