title: [Expression of peroxisome proliferator-activated receptor gamma in glioma].
abstract: OBJECTIVE
  To investigate the expression and significance of peroxisome proliferators-activated receptor gamma (PPAR gamma) in human glioma.
  
  
  METHODS
  Immunohistochemical staining for PPAR gamma was performed using biopsy specimens of human glioma of various histological types. Expression of PPAR gamma and GFAP in glioma cell lines SWO-38, U251 and SHG-44 were analyzed using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Immunohistochemical study showed that PPAR gamma was expressed in glioma tissues with positive rate of 37.5%. Western blotting and RT-PCR showed that PPAR gamma was expressed in both glioma cell lines SWO-38 and U251, but not in SHG-44 cells. However, high expression of GFAP was detected in SHG-44 cells.
  
  
  CONCLUSIONS
  PPAR gamma is associated with carcinogens of glioma. Actived PPAR gamma by agonist may be a novel approach to the treatment of glioma.
authors: Department of Pathology, Chongqing Medical Oniversity, Chongqing 410016, China. wangmhhh@yahoo.cn
authors: Ming-hua
authors: MH
authors: Wang
authors: Xue-yun
authors: XY
authors: Zhong
authors: Chen-li
authors: CL
authors: Lin
authors: You-ke
authors: YK
authors: Xie
authors: Jin-ping
authors: JP
authors: Jia
authors: Su-mei
authors: SM
authors: Li
authors: Can
authors: C
authors: Mi
mesh_terms: D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005904:Glial Fibrillary Acidic Protein; D047495:PPAR gamma; D012333:RNA, Messenger