Proteomic analysis of selenium embryotoxicity in cultured postimplantation rat embryos.
BACKGROUND
Developmental toxicity of selenium (Se) is a nutritional, environmental and medicinal concern. Here, we investigated Se embryotoxicity by proteomic analysis of cultured rat embryos.


METHODS
Rat embryos at day 9.5 or 10.5 of gestation were cultured for 48 or 24 h, respectively, in the presence of sodium selenate (100 or 150 microM) or sodium selenite (20 or 30 microM). Proteins from the embryo proper and yolk sac membrane were analyzed by two-dimensional electrophoresis for quantitative changes from those in control embryos. Proteins with quantitative changes were identified by mass spectrometric analysis.


RESULTS
Growth inhibition and morphological abnormalities of cultured embryos were observed in all the Se treatment groups. By the analysis of the embryo proper, actin-binding proteins were identified as proteins with quantitative changes by selenate: increased phosphorylated-cofilin 1, increased phosphorylated-destrin, decreased drebrin E, and decreased myosin light polypeptide 3. Many proteins showed similar changes between selenate and selenite, including increased ATP-synthase, decreased acidic ribosomal phosphoprotein P0, and decreased pyrroline-5-carboxylate reductase-like. In the yolk sac membrane, antioxidant proteins were identified for protein spots with quantitative changes by selenite: increased peroxiredoxin 1 and increased glutathione S-transferase.


CONCLUSIONS
The identified proteins with quantitative changes by selenate or selenite were considered to be candidate proteins involved in Se embryotoxicity: the actin-binding proteins for selenate embryotoxicity, proteins with the similar changes for the common Se embryotoxicity and antioxidant proteins for modification of Se embryotoxicity by redox-related treatments. These proteins may also be used as biomarkers in developmental toxicity studies.
Usami
Makoto
M
Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan. usami@nihs.go.jp
Mitsunaga
Katsuyoshi
K
Nakazawa
Ken
K
Doi
Osamu
O
D000818:Animals; D002478:Cells, Cultured; D051338:Cofilin 1 / Q000378:metabolism; D051345:Destrin / Q000378:metabolism; D004527:Egg Proteins / Q000187:drug effects / Q000378:metabolism; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011247:Pregnancy; D011494:Protein Kinases / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000187:drug effects; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D064586:Selenic Acid; D012643:Selenium / Q000633:toxicity*; D018036:Selenium Compounds / Q000494:pharmacology / Q000633:toxicity; D018038:Sodium Selenite / Q000494:pharmacology / Q000633:toxicity; D015017:Yolk Sac / Q000737:chemistry / Q000187:drug effects
C495812:Cfl1 protein, rat; D051338:Cofilin 1; D051345:Destrin; D004527:Egg Proteins; D008565:Membrane Proteins; D020543:Proteome; D018036:Selenium Compounds; D011494:Protein Kinases; D012643:Selenium; D018038:Sodium Selenite; D064586:Selenic Acid