Heterologous expression of pyruvate decarboxylase in Geobacillus thermoglucosidasius.
Expression of a pyruvate decarboxylase (Pdc) pathway in metabolically versatile thermophilic bacteria could create novel ethanologenic organisms, but no suitable thermostable Pdc is available. We have demonstrated that Pdc from Zymomonas mobilis can be expressed in an active form in Geobacillus thermoglucosidasius at up to 52 degrees C, while expression of Pdc polypeptides up to 54 degrees C was evident from Western blotting. By using an unstable lactate dehydrogenase (ldh) mutant of G. thermoglucosidasius, indirect evidence of Pdc activity in vivo was also obtained.
Thompson
Ann H
AH
Division of Biology, Faculty of Natural Sciences, Imperial College London, South Kensington, London SW7 2AZ, UK.
Studholme
David J
DJ
Green
Edward M
EM
Leak
David J
DJ
D001407:Bacillus / Q000201:enzymology*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000235:genetics; D019344:Lactic Acid / Q000378:metabolism; D011767:Pyruvate Decarboxylase / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D017355:Zymomonas / Q000201:enzymology
D019344:Lactic Acid; D019289:Pyruvic Acid; D011767:Pyruvate Decarboxylase