Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans.
Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains ORS 571 and AA-SK-5 by means of imbibition. All treated calli had significant increases in protein content, total nitrogen and nitrogenase activity. Imbibition of ORS 571 had significant biochemical effect on the developing calli than AA-SK-5. The crop response study from the regenerated plantlets showed a positive correlation in yield than uninoculated control. The endophytic colonization was observed in all parts of the plants analyzed. Further, colonization was also confirmed by microtome sectioning.
Senthilkumar
M
M
Department of Agricultural Microbiology, Horticultural College and Research Institute, Tamilnadu Agricultural University, Periyakulam 625 604, Tamilnadu, India. senthiltnj@rediffmail.com
Madhaiyan
M
M
Sundaram
S P
SP
Sangeetha
H
H
Kannaiyan
S
S
D020599:Azorhizobium caulinodans / Q000166:cytology / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D018556:Crops, Agricultural / Q000166:cytology / Q000187:drug effects; D003470:Culture Media; D007701:Kinetin / Q000494:pharmacology; D012275:Oryza / Q000166:cytology / Q000187:drug effects / Q000382:microbiology*; D018547:Plant Stems / Q000187:drug effects / Q000382:microbiology; D012038:Regeneration / Q000187:drug effects; D036226:Seedlings / Q000382:microbiology; D013559:Symbiosis / Q000187:drug effects; D046509:Tissue Culture Techniques
D003470:Culture Media; D007701:Kinetin