Functional reinnervation of the canine bladder after spinal root transection and immediate somatic nerve transfer.
This study was performed to determine whether nerve transfer immediately after spinal root transection would lead to bladder reinnervation in a canine model. In one animal, the left T12 intercostal nerve was mobilized, cut and attached to the severed ends of sacral roots inducing bladder contraction using a graft from the T11 intercostal nerve. On the right side and bilaterally in two other dogs, coccygeal roots innervating tail musculature were cut and attached to the severed bladder sacral roots (coccygeal nerve transfer [CG NT]). In four other dogs, bladder sacral roots were transected in the vertebral column, and the genitofemoral nerve was transferred within the abdomen to the pelvic nerve (genitofemoral nerve transfer [GF NT]). After 14 months for CG NT and 4.5 months for GF NT, electrical stimulation of the pelvic nerve induced bladder pressure and urethral fluid flow on the intercostal nerve transfer side, in each of the five CG NT sites and bilaterally in three of the four GF NT animals. Reinnervation was further shown by retrograde labeling of spinal cord neurons following fluorogold injections into the bladder wall and by histological examination of the root/nerve suture sites. In all CG NT animals, labeled neuronal cell bodies were located in ventral horns in lamina IX of coccygeal cord segments. In the three GF NT animals in which pelvic nerve stimulation induced bladder contraction, abundant labeled cell bodies were observed in lamina IX and lateral zona intermedia of upper lumbar cord. These results clearly demonstrate that bladder reinnervation can be accomplished by immediate nerve transfer of intercostal nerves or coccygeal spinal roots to severed bladder sacral roots, or by transfer of peripheral genitofemoral nerves (L1,2 origin) to pelvic nerves.
Ruggieri
Michael R
MR
Department of Urology, Temple University, Philadelphia, Pennsylvania 19140-5104, USA. rugg@temple.edu
Braverman
Alan S
AS
D'Andrea
Linda
L
McCarthy
James
J
Barbe
Mary F
MF
D000818:Animals; D004285:Dogs; D004558:Electric Stimulation; D005260:Female; D009416:Nerve Regeneration / Q000502:physiology*; D016067:Nerve Transfer / Q000379:methods*; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000502:physiology; D011843:Radiculopathy / Q000503:physiopathology / Q000601:surgery*; D020127:Recovery of Function / Q000502:physiology; D012447:Sacrum; D013119:Spinal Cord Injuries / Q000503:physiopathology / Q000601:surgery; D013126:Spinal Nerve Roots / Q000293:injuries / Q000503:physiopathology / Q000601:surgery*; D013127:Spinal Nerves / Q000033:anatomy & histology / Q000502:physiology / Q000601:surgery; D013904:Thoracic Vertebrae; D001743:Urinary Bladder / Q000294:innervation* / Q000503:physiopathology; D001750:Urinary Bladder, Neurogenic / Q000209:etiology / Q000503:physiopathology / Q000601:surgery*; D014554:Urination / Q000502:physiology; D014855:Wallerian Degeneration / Q000503:physiopathology / Q000517:prevention & control / Q000601:surgery