title: Fractionation of proteins by immobilized metal affinity chromatography.
abstract: It is widely known that the progress of proteomics mostly depends on the development of more advanced and sensitive protein separation technologies. Immobilized metal affinity chromatography (IMAC) is a useful protein fractionation method used to enrich metal associated proteins. IMAC represents an affinity separation approach based on the interaction between proteins and metal ions immobilized on a solid support. By changing various metal ions and other experimental conditions such as pH and elution composition, IMAC can selectively isolate metal-binding protein fractions for further specific proteomic analysis. The combination of IMAC with other protein analytical technologies has been successfully applied in characterizing metalloproteome and post-translational modifications. In the future, newly developed IMAC techniques integrated with other proteomic methods will significantly contribute to the further development of proteomics.
authors: The University of Hong Kong, Hong Kong, China.
authors: Xuesong
authors: X
authors: Sun
authors: Jen-Fu
authors: JF
authors: Chiu
authors: Qing-Yu
authors: QY
authors: He
mesh_terms: D000818:Animals; D002413:Cations, Divalent / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019216:Metals, Heavy / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*
chemical_list: D002413:Cations, Divalent; D019216:Metals, Heavy; D011506:Proteins; D020543:Proteome