A PCR-based method for the identification of important wood rotting fungal taxa within Ganoderma, Inonotus s.l. and Phellinus s.l.
Two multiplex PCRs, based on 10 taxon-specific primers designed on rRNA gene regions, were developed for the identification of taxa within the lignivorous genera Ganoderma, Inonotus s.l. and Phellinus s.l., each comprising both secondary and primary aggressive decay fungi. Each multiplex PCR proved to correctly identify 1 x 10(-2) pg of fungal target DNA directly from wood. This method can be helpful in detecting decay in standing trees independent of its stage of advancement, and to identify the associated decay agents.
Guglielmo
Fabio
F
Department of Exploitation and Protection of the Agricultural and Forestry Resources, University of Torino, Grugliasco, TO, Italy.
Gonthier
Paolo
P
Garbelotto
Matteo
M
Nicolotti
Giovanni
G
D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D016468:Forestry; D005658:Fungi / Q000235:genetics / Q000302:isolation & purification; D032902:Ganoderma / Q000145:classification* / Q000235:genetics; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D014934:Wood / Q000382:microbiology*
D017931:DNA Primers; D004271:DNA, Fungal