title: Accuracy of FISH analysis in predicting chromosomal status in patients undergoing preimplantation genetic diagnosis.
abstract: OBJECTIVE
  The purpose of this study was to determine the positive predictive value (PPV) and negative predictive value (NPV) of FISH analysis and to determine which chromosomal abnormalities are most frequently confirmed.
  
  
  METHODS
  Prospective observational.
  
  
  METHODS
  IVF laboratory.
  
  
  METHODS
  Two hundred forty-one embryos were analyzed from 98 patients.
  
  
  METHODS
  FISH reanalysis.
  
  
  METHODS
  Embryos that would have been discarded in patients undergoing preimplantation genetic diagnosis (PGD) were fixed and FISH reanalysis was performed. Results of reanalysis were compared with the day 3 diagnosis while PPV and NPV were calculated.
  
  
  RESULTS
  Among the 241 embryos, 198 embryos were abnormal and 43 were normal by day 3 FISH analysis. The PPV was 83% and the NPV was 81%. PPV was also determined for specific categories of aneuploidy, and certain abnormalities such as monosomies, trisomies, tetrasomies, and polyploidies were frequently confirmed on reanalysis (PPV >80%), whereas Turner syndrome diagnosis was not (PPV = 17%).
  
  
  CONCLUSIONS
  FISH analysis offers a PPV of 83% and NPV of 81% when evaluating a single blastomere in conjunction with PGD. FISH errors and mosaicism are primarily responsible for the errors associated with FISH analysis in PGD.
authors: Pacific Fertility Center, Los Angeles, California 90024, USA. cmdeugarte@hotmail.com
authors: Catherine M
authors: CM
authors: DeUgarte
authors: Man
authors: M
authors: Li
authors: Mark
authors: M
authors: Surrey
authors: Hal
authors: H
authors: Danzer
authors: David
authors: D
authors: Hill
authors: Alan H
authors: AH
authors: DeCherney
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000196:embryology* / Q000235:genetics*; D002874:Chromosome Mapping / Q000706:statistics & numerical data*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000706:statistics & numerical data*; D008297:Male; D019836:Preimplantation Diagnosis / Q000706:statistics & numerical data*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity