Detection of siRNAs and miRNAs.
Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.
Kauppinen
Sakari
S
Wilhelm Johannsen Center for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Havelda
Zoltán
Z
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D020868:Gene Silencing; D017403:In Situ Hybridization / Q000379:methods; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D015345:Oligonucleotide Probes; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000821:virology; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
D035683:MicroRNAs; D015345:Oligonucleotide Probes; D034741:RNA, Small Interfering; D012367:RNA, Viral