Phytate utilization by genetically engineered lysine-producing Corynebacterium glutamicum.
Heterologous expression of a phytase gene (phyC) from Bacillus amyloliquefaciens DSM 7 enabled the growth of Corynebacterium glutamicum with phytate (myo-inositol-1,2,3,4,5,6-hexakisphosphate) as a new, sole source of phosphorus. Phytate was not used as a carbon source. During growth of the phyC-expressing amino acid (l-lysine)-producing strain C. glutamicum ATCC 21253 (pWLQ2::phyC) with phytate as the source of phosphorus, merely a small, transient accumulation of inorganic phosphate was observed in the fermentation broth. At the later stages of fermentation, free inorganic phosphate from phytate degradation was no longer detectable. Growth and l-lysine production by the phytase-producing strain C. glutamicum ATCC 21253 (pWLQ2::phyC) in phytate medium did not differ significantly from control experiments with strain C. glutamicum ATCC 21253 (pWLQ2) conducted with an excess of inorganic phosphate, demonstrating that there was no phosphate limitation when phytate was used as the phosphorus source. Under the expression conditions employed, only part of PhyC was secreted to the culture broth by C. glutamicum, but this did not significantly affect growth or lysine production.
Tzvetkov
Mladen V
MV
Institut für Mikrobiologie und Genetik, Georg-August-Universität, Göttingen, Germany.
Liebl
Wolfgang
W
D010832:6-Phytase / Q000235:genetics* / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology / Q000235:genetics; D048230:Corynebacterium glutamicum / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D003470:Culture Media / Q000378:metabolism; D005818:Genetic Engineering; D005822:Genetic Vectors; D008239:Lysine / Q000032:analysis / Q000096:biosynthesis; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism; D010833:Phytic Acid / Q000378:metabolism*; D014169:Transformation, Bacterial; D019076:Transgenes / Q000502:physiology
D003470:Culture Media; D010710:Phosphates; D010758:Phosphorus; D010833:Phytic Acid; D010832:6-Phytase; D008239:Lysine