title: Mesoporous silica nanoparticles inhibit cellular respiration.
abstract: We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500 microg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 microg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 microg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.
authors: Department of Pediatrics, State University of New York, Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA.
authors: Zhimin
authors: Z
authors: Tao
authors: Matthew P
authors: MP
authors: Morrow
authors: Tewodros
authors: T
authors: Asefa
authors: Krishna K
authors: KK
authors: Sharma
authors: Cole
authors: C
authors: Duncan
authors: Abhishek
authors: A
authors: Anan
authors: Harvey S
authors: HS
authors: Penefsky
authors: Jerry
authors: J
authors: Goodisman
authors: Abdul-Kader
authors: AK
authors: Souid
mesh_terms: D002460:Cell Line; D019069:Cell Respiration / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000494:pharmacology*
chemical_list: D012822:Silicon Dioxide