title: Microarray expression profiling of human dental pulp from single subject.
abstract: BACKGROUND
  Microarray is a recently developed simultaneous analysis of expression patterns of thousand of genes. The aim of this research was to evaluate the expression profile of human healthy dental pulp in order to find the presence of genes activated and encoding for proteins involved in the physiological process of human dental pulp. We report data obtained by analyzing expression profiles of human tooth pulp from single subjects, using an approach based on the amplification of the total RNA.
  
  
  METHODS
  Experiments were performed on a high-density array able to analyse about 21,000 oligonucleotide sequences of about 70 bases in duplicate, using an approach based on the amplification of the total RNA from the pulp of a single tooth. Obtained data were analyzed using the S.A.M. system (Significance Analysis of Microarray) and genes were merged according to their molecular functions and biological process by the Onto-Express software.
  
  
  RESULTS
  The microarray analysis revealed 362 genes with specific pulp expression. Genes showing significant high expression were classified in genes involved in tooth development, protoncogenes, genes of collagen, DNAse, Metallopeptidases and Growth factors.
  
  
  CONCLUSIONS
  We report a microarray analysis, carried out by extraction of total RNA from specimens of healthy human dental pulp tissue. This approach represents a powerful tool in the study of human normal and pathological pulp, allowing minimization of the genetic variability due to the pooling of samples from different individuals.
authors: Department of Oral Science, University G. d' Annunzio, Chieti, Italy.
authors: Stefano
authors: S
authors: Tete
authors: Filiberto
authors: F
authors: Mastrangelo
authors: Anna Paola
authors: AP
authors: Scioletti
authors: Michelangelo
authors: M
authors: Tranasi
authors: Florina
authors: F
authors: Raicu
authors: Michele
authors: M
authors: Paolantonio
authors: Liborio
authors: L
authors: Stuppia
authors: Raffaele
authors: R
authors: Vinci
authors: Enrico
authors: E
authors: Gherlone
authors: Cristian
authors: C
authors: Ciampoli
authors: Maria Teresa
authors: MT
authors: Sberna
authors: Pio
authors: P
authors: Conti
mesh_terms: D000293:Adolescent; D000483:Alleles; D030541:Databases, Genetic; D003782:Dental Pulp / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D014644:Genetic Variation; D006801:Humans; D046228:Microarray Analysis; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000378:metabolism; D012984:Software
chemical_list: D012313:RNA