Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts.
The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.
Pacholczyk
Gabriela
G
Mouse Embryonic Stem Cell & Transgenesis Core Laboratories, Medical College of Georgia, Augusta, GA, USA.
Suhag
Rupali
R
Mazurek
Magdalena
M
Dederscheck
Suzanne M
SM
Koni
Pandelakis A
PA
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000502:physiology*; D005818:Genetic Engineering / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout / Q000196:embryology / Q000235:genetics* / Q000254:growth & development*