Comparative expression analysis of the renin-angiotensin system components between white and brown perivascular adipose tissue.
Recent studies have demonstrated that the rat adipose tissue expresses some of the components necessary for the production of angiotensin II (Ang II) and the receptors mediating its actions. The aim of this work is to characterize the expression of the renin-angiotensin system (RAS) components in perivascular adipose tissue and to assess differences in the expression pattern depending on the vascular bed and type of adipose tissue. We analyzed Ang I and Ang II levels as well as mRNA levels of RAS components by a quantitative RT-PCR method in periaortic (PAT) and mesenteric adipose tissue (MAT) of 3-month-old male Wistar-Kyoto rats. PAT was identified as brown adipose tissue expressing uncoupling protein-1 (UCP-1). It had smaller adipocytes than those from MAT, which was identified as white adipose tissue. All RAS components, except renin, were detected in both PAT and MAT. Levels of expression of angiotensinogen, Ang-converting enzyme (ACE), and ACE2 were similar between PAT and MAT. Renin receptor expression was five times higher, whereas expression of chymase, AT(1a), and AT(2) receptors were significantly lower in PAT compared with MAT respectively. In addition, three isoforms of the AT(1a) receptor were found in perivascular adipose tissue. The AT(1b) receptor was found at very a low expression level. Ang II levels were higher in MAT with no differences between tissues in Ang I. The results show that the RAS is differentially expressed in white and brown perivascular adipose tissues implicating a different role for the system depending on the vascular bed and the type of adipose tissue.
Gálvez-Prieto
B
B
Unidad de Cartografía Cerebral, Instituto Pluridisciplinar Universidad Complutense, Juan XXIII 1, 28040, Madrid Spain.
Bolbrinker
J
J
Stucchi
P
P
de Las Heras
A I
AI
Merino
B
B
Arribas
S
S
Ruiz-Gayo
M
M
Huber
M
M
Wehland
M
M
Kreutz
R
R
Fernandez-Alfonso
M S
MS
D002001:Adipose Tissue, Brown / Q000737:chemistry*; D052436:Adipose Tissue, White / Q000737:chemistry*; D000803:Angiotensin I / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D000818:Animals; D007473:Ion Channels / Q000032:analysis; D008297:Male; D024101:Mitochondrial Proteins / Q000032:analysis; D007703:Peptidyl-Dipeptidase A / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011921:Rats, Inbred WKY; D044139:Receptor, Angiotensin, Type 2 / Q000032:analysis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics; D012084:Renin-Angiotensin System; D000071256:Uncoupling Protein 1; D000093342:Prorenin Receptor
D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D044139:Receptor, Angiotensin, Type 2; D011956:Receptors, Cell Surface; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1; D000804:Angiotensin II; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D000093342:Prorenin Receptor