Production and characterization of immortal human neural stem cell line with multipotent differentiation property.
We document the protocols and methods for the production of immortalized cell lines of human neural stem cells from the human fetal central nervous system (CNS) cells by using a retroviral vector encoding v-myc oncogene. One of the human neural stem cell lines (HB1.F3) was found to express nestin and other specific markers for human neural stem cells, giving rise to three fundamental cell types of the CNS: neurons, astrocytes, and oligodendrocytes. After transplantation into the brain of mouse model of stroke, implanted human neural stem cells were observed to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells.
Kim
Seung U
SU
Division of Neurology, UBC Hospital, University of British Columbia, Vancouver, BC, Canada.
Nagai
Atsushi
A
Nakagawa
Eiji
E
Choi
Hyun B
HB
Bang
Jung H
JH
Lee
Hong J
HJ
Lee
Myung A
MA
Lee
Yong B
YB
Park
In H
IH
D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D020732:Cytogenetic Analysis; D005333:Fetus / Q000166:cytology; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009928:Organ Specificity; D012190:Retroviridae; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase