title: Production and characterization of immortal human neural stem cell line with multipotent differentiation property.
abstract: We document the protocols and methods for the production of immortalized cell lines of human neural stem cells from the human fetal central nervous system (CNS) cells by using a retroviral vector encoding v-myc oncogene. One of the human neural stem cell lines (HB1.F3) was found to express nestin and other specific markers for human neural stem cells, giving rise to three fundamental cell types of the CNS: neurons, astrocytes, and oligodendrocytes. After transplantation into the brain of mouse model of stroke, implanted human neural stem cells were observed to migrate extensively from the site of implantation into other anatomical sites and to differentiate into neurons and glial cells.
authors: Division of Neurology, UBC Hospital, University of British Columbia, Vancouver, BC, Canada.
authors: Seung U
authors: SU
authors: Kim
authors: Atsushi
authors: A
authors: Nagai
authors: Eiji
authors: E
authors: Nakagawa
authors: Hyun B
authors: HB
authors: Choi
authors: Jung H
authors: JH
authors: Bang
authors: Hong J
authors: HJ
authors: Lee
authors: Myung A
authors: MA
authors: Lee
authors: Yong B
authors: YB
authors: Lee
authors: In H
authors: IH
authors: Park
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D020732:Cytogenetic Analysis; D005333:Fetus / Q000166:cytology; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009928:Organ Specificity; D012190:Retroviridae; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase