title: Determination of natural olive fruit fly pheromone in insect samples by enzyme linked immunoassays.
abstract: The olive fruit fly pheromone avidin-biotin ELISA immunoassay, based on the use of polyclonal G antibodies derived from rabbits (reported previously) and a newer assay, based on the use of polyclonal Y antibodies isolated from the eggs of laying hens (reported in this paper), were applied successfully for the analysis of natural pheromone in virgin adult female olive fruit flies. According to the results obtained, the pheromone content in the glands of adult female olive fruit flies increases from the third to the ninth day of their age. During the calling period, the female olive fruit flies seem to emit approximately 1.1microg pheromone/insect/day at least. The immunoassay, based on the Y antibodies, is slightly more sensitive (detection limit 40ng/mL) than the assay based on polyclonal anti-pheromone rabbit antiserum (detection limit 80ng/mL). As revealed by thorough cross-reactivity studies, including 14 structurally similar to the olive fruit fly pheromone molecules, the newer immunoassay is less selective than the previous one and seems to cross react with few molecules bearing the spiroketal moiety.
authors: NCSR Demokritos, RRP Institute, Immunopeptide Chemistry Laboratory, Aghia Paraskevi, 153 10 Athens, Greece.
authors: Afroditi
authors: A
authors: Neokosmidi
authors: Valentine
authors: V
authors: Ragoussis
authors: Christos
authors: C
authors: Zikos
authors: Maria
authors: M
authors: Paravatou-Petsotas
authors: Evangelia
authors: E
authors: Livaniou
authors: Nikitas
authors: N
authors: Ragoussis
authors: Gregory
authors: G
authors: Evangelatos
mesh_terms: D000818:Animals; D003429:Cross Reactions; D004175:Diptera; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D010675:Pheromones / Q000032:analysis* / Q000276:immunology
chemical_list: D010675:Pheromones