title: Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation.
abstract: OBJECTIVE
  To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion.
  
  
  METHODS
  The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.
  
  
  RESULTS
  p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.
  
  
  CONCLUSIONS
  p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
authors: Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
authors: Rui
authors: R
authors: Fei
authors: Yu
authors: Y
authors: Fei
authors: Sheng
authors: S
authors: Zheng
authors: Yan-guang
authors: YG
authors: Gao
authors: Hong-xia
authors: HX
authors: Sun
authors: Xian-lu
authors: XL
authors: Zeng
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D020441:Ginkgo biloba / Q000276:immunology* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects*; D019007:P-Selectin / Q000276:immunology* / Q000378:metabolism; D018515:Plant Leaves / Q000276:immunology* / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D019007:P-Selectin; D011134:Polysaccharides