title: [Effect of the self-etching adhesives system on human pulp fibroblast].
abstract: OBJECTIVE
  To compare and evaluate the biocompatibility of three kinds of dentin bonding agents Xeno III (XO), Adper Prompt (AP), Single bond2 (SB) through cell culture in vitro.
  
  
  METHODS
  Three kinds of dentin bonding agents (XO, AP, SB) were applied on the surface of the dental slices which were 5.0 mm in diameter and 0.5 mm in depth. By immersing the slices into the DMEM culture medium, the maceration extracts were obtained. Normal dental pulps of teenagers were collected and human pulp fibroblast was cultured using tissue explant method. The fifth generation pulp cells were exposed to culture medium containing different concentrations of maceration extracts (100.0%, 50.0%, 25.0%, 12.5%) for 24, 72, 120 h. At last, MTT method was used to evaluate the cytotoxicity of the dentin bonding agents on human pulp fibroblast.
  
  
  RESULTS
  The results showed that all three kinds of dentin bonding systems had cytotoxicity to human pulp fibroblast in different degree in vitro. The cytotoxicity of XO and AP was less than SB. The difference was statistically significant (P<0.05).
  
  
  CONCLUSIONS
  The results of cell culture in vitro indicated that total-etching adhesives system has more irritation to pulp than self-etching adhesives system.
authors: Department of Endodontics, The Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China.
authors: Ming
authors: M
authors: Zhang
authors: Yan
authors: Y
authors: Feng
authors: Xiao-jing
authors: XJ
authors: Huang
authors: Li-shan
authors: LS
authors: Lei
authors: Bi-qiong
authors: BQ
authors: Zheng
authors: You-guang
authors: YG
authors: Lu
mesh_terms: D000269:Adhesives; D000293:Adolescent; D003782:Dental Pulp; D003804:Dentin; D017220:Dentin-Bonding Agents; D005347:Fibroblasts; D006801:Humans; D019279:Resin Cements
chemical_list: D000269:Adhesives; D017220:Dentin-Bonding Agents; D019279:Resin Cements; C487957:Xeno III