Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip.
The measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented.
Hromada
Louis P
LP
University of Maryland, College Park, Department of Mechanical Engineering, College Park, Maryland 20742, USA.
Nablo
Brian J
BJ
Kasianowicz
John J
JJ
Gaitan
Michael A
MA
DeVoe
Don L
DL
D001427:Bacterial Toxins; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006460:Hemolysin Proteins / Q000032:analysis* / Q000378:metabolism; D007473:Ion Channels / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D011092:Polyethylene Glycols / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D018030:Silver Compounds; C021091:staphylococcal alpha-toxin; D012834:Silver; D011092:Polyethylene Glycols; C037548:silver chloride