title: [Matrine-induced apoptosis in human colon adenocarcinoma SW620 cells].
abstract: OBJECTIVE
  To investigate the effects of matrine on the cell cycle and apoptosis in human colon adenocarcinoma SW620 cells and explore the possible mechanisms.
  
  
  METHODS
  The effect of matrine on cell proliferation was assessed using MTT assay, and the cell cycle arrest induced by matrine was determined by flow cytometry. The changes of cell morphology were observed through optical microscope, fluorescence microscope and electron microscope, and the cell apoptosis was detected using Annexin V-FITC apoptosis assay.
  
  
  RESULTS
  Matrine inhibited the proliferation of SW620 cells in a dose- and time-dependent manner. Compared with the control group, the matrine-treated cells showed increased cell percentage arrested in G 0/G1 phase with decreased S-phase cells. Morphologically, the SW620 cells treated with matrine exhibited cell shrinkage, cell size reduction, plasma condensation, cytoplasmic vacuolar changes, and formation of apoptotic body with also the presence of the signet-ring cells, all typical of apoptotic cells.
  
  
  CONCLUSIONS
  Matrine exposure of SW620 cells inhibits the cell proliferation, causes cell cycle arrest at G 0/G1 phase, and induces apoptosis in a dose- and time- dependent manner.
authors: Department of Pathology, Southern Medical University, Guangzhou 510515, China. wangxiaoyan639@163.com
authors: Xiao-yan
authors: XY
authors: Wang
authors: Lei
authors: L
authors: Liang
authors: Hong-zhu
authors: HZ
authors: Deng
authors: Wang-jun
authors: WJ
authors: Liao
authors: Zu-guo
authors: ZG
authors: Li
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000648:ultrastructure; D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000473:pathology / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D011807:Quinolizines / Q000494:pharmacology*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D000093842:Matrines