title: Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis.
abstract: OBJECTIVE
  To study the effect of the modulation of inositol hexaphosphate (IP6) in the biological immunohistochemistry expression of cellular signaling marker apoptosis, in model of carcinogenesis of colon induced by azoxymethane (AOM).
  
  
  METHODS
  Wistar rats (N=112) distributed in 4 groups (n=28): Control; B, AOM (5 mg kg-1, 2x, to break week 3); C, IP6 (in water 1%, six weeks); D, IP6+AOM. Weekly euthanasia (n=7), from week three. Immunohistochemistry of ascendant colon with biological marker inositol 1,4,5 triphosphate receptor type III (Itpr3). Quantification of the immune-expression with use of computer-assisted image processing. Analysis statistics of the means between groups, weeks in groups, groups in weeks, and established significance when p<or=0.05.
  
  
  RESULTS
  One proved significant difference between groups in the expression of Itpr3, p<0.0001; with Itpr3 reduction of BxD group, p<0.001.
  
  
  CONCLUSIONS
  Inositol hexaphosphate promotes modulation of biological markers with reduction of Itpr3 in carcinogenesis of colon.
authors: Program in Surgery and Experimentation, Department of Surgery, UNIFESP-SP, São Paulo, Brazil. gmarks@brturbo.com.br
authors: Marks
authors: M
authors: Guido
authors: Djalma José
authors: DJ
authors: Fagundes
authors: Celso Massaschi
authors: CM
authors: Ynouye
authors: Elenir Rose Jardim Cury
authors: ER
authors: Pontes
authors: Luiz Carlos
authors: LC
authors: Takita
authors: Eva Glória
authors: EG
authors: Siufi do Amaral
authors: Roberto
authors: R
authors: Teruya
authors: Manoel Catarino
authors: MC
authors: Paes
authors: José Lacerda
authors: JL
authors: Brasileiro
authors: Ricardo Dutra
authors: RD
authors: Aydos
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001397:Azoxymethane; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002273:Carcinogens; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism*; D008297:Male; D010833:Phytic Acid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D014408:Biomarkers, Tumor; D002273:Carcinogens; D053496:Inositol 1,4,5-Trisphosphate Receptors; D010833:Phytic Acid; D001397:Azoxymethane