Effect of anti-inflammatory agents on the integration of autogenous bone graft and bovine bone devitalized matrix in rats.
OBJECTIVE
To study the repair of bone defect filled with autograft or bovine bone devitalized matrix in rats under anti-inflammatory action.


METHODS
Two hundred and forty Wistar rats were distributed to two groups of 120 animals each. A 2mm-diameter defect was created in the femoral diaphysis. Animals of Group I had the bone defect filled with autograft and those of Group II, with bovine bone devitalized matrix. Animals of each group were redistributed to four subgroups according to the intramuscular administration of anti-inflammatory drug or saline solution: A - diclofenac sodium; B - dexamethasone; C - meloxicam; D - saline solution. Evaluation periods were 7, 14 and 30 days. Histological evaluation consisted of quantifying the inflammatory process, the bone neoformation, the collagen formation and the presence of macrophages.


RESULTS
Animals of Group I did not show significant difference considering inflammatory reaction. Significant and progressive increase of bone neoformation was observed in both groups. The animals that received meloxicam and autograft showed less collagen formation at 14 and 30 days. The number of macrophages was higher in Group II than in Group I. The animals treated with dexamethasone and saline solution did not show statistically significant difference.


CONCLUSIONS
Diclofenac sodium and meloxicam delayed bone graft repair and dexamethasone did not interfere in it.
Silva
Roberto Antoniolli da
RA
Department of Surgical Clinics, Federal University of Mato Grosso do SulMato Grosso do Sul, Brazil. ianterra@terra.com.br
Fagundes
Djalma José
DJ
Antoniolli Silva
Andréia Conceição Milan Brochado
AC
Sisti
Karin Ellen
KE
Brochado de Carvalho
Themis Maria Milan
TM
Silva
Daniel Nunes e
DN
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016025:Bone Transplantation; D002417:Cattle; D003907:Dexamethasone / Q000494:pharmacology*; D004008:Diclofenac / Q000494:pharmacology*; D008297:Male; D000077239:Meloxicam; D016348:Osseointegration / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013843:Thiazines / Q000494:pharmacology*; D013844:Thiazoles / Q000494:pharmacology*; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D013843:Thiazines; D013844:Thiazoles; D004008:Diclofenac; D003907:Dexamethasone; D000077239:Meloxicam