The aptamer core of SAM-IV riboswitches mimics the ligand-binding site of SAM-I riboswitches.
A novel family of riboswitches, called SAM-IV, is the fourth distinct set of mRNA elements to be reported that regulate gene expression via direct sensing of S-adenosylmethionine (SAM or AdoMet). SAM-IV riboswitches share conserved nucleotide positions with the previously described SAM-I riboswitches, despite rearranged structures and nucleotide positions with family-specific nucleotide identities. Sequence analysis and molecular recognition experiments suggest that SAM-I and SAM-IV riboswitches share similar ligand binding sites, but have different scaffolds. Our findings support the view that RNA has considerable structural versatility and reveal that riboswitches exploit this potential to expand the scope of RNA in genetic regulation.
Weinberg
Zasha
Z
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Regulski
Elizabeth E
EE
Hammond
Ming C
MC
Barrick
Jeffrey E
JE
Yao
Zizhen
Z
Ruzzo
Walter L
WL
Breaker
Ronald R
RR
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D008024:Ligands; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism
D052157:Aptamers, Nucleotide; D017931:DNA Primers; D008024:Ligands; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012436:S-Adenosylmethionine