[Investigation of mechanisms of synaptic vesicles fusion with acceptor membranes in the model of exocytosis].
Fusion of synaptic vesicles with various target membranes was investigated on the cell-free model system that reflects the final step of exocytosis. Plasma membranes, synaptic vesicles and liposomes were used as acceptor membranes. The process of membrane fusion was triggered by Ca2+. We have demonstrated that synaptic vesicles are prone to fuse with liposomes in buffer solution. This process was strongly dependent on ionic force of medium and phospholipid composition of liposomes. Cytosolic proteins of synaptosomes inhibited the fusion of synaptic vesicles with liposomes, while these were required for the fusion of synaptic vesicles with native membrane structures. Trypsinolysis of acceptor membranes markedly inhibited the fusion response. It means protein components of target membrane are necessary for realization of the final step of exocytosis.
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D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002474:Cell-Free System; D005089:Exocytosis / Q000502:physiology*; D008561:Membrane Fusion / Q000502:physiology*; D051381:Rats; D050600:SNARE Proteins / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism / Q000502:physiology*; D013572:Synaptic Vesicles / Q000378:metabolism / Q000502:physiology*; D013574:Synaptosomes / Q000378:metabolism / Q000502:physiology
D050600:SNARE Proteins; D000255:Adenosine Triphosphate; D002118:Calcium