Serum constituents analyses in dairy cows: effects of duration and temperature of the storage of clotted blood.
We studied the effects of storage time and temperature of clotted whole blood on the amounts of 17 analytes in bovine blood serum. Serum separated after blood was allowed to stand for 2, 4, 6, 12 and 24h at room temperature or on ice. Results obtained for phosphorous, magnesium, urea, cholesterol, beta-hydroxybutyrate (BHBA), triglyceride, albumin, total protein and gamma-glutamyletransferase (GGT) were not influenced by storage at room temperature or on ice for as long as 24h. Duration of the clotted whole blood storage had a significant effect on calcium, glucose concentrations, creatine kinase (CK) and aspartate aminotransferase (AST) activities and temperature had a significant effect on glucose, non-esterified fatty acids, CK and bilirubin concentrations.
Ehsani
A
A
Center of Excellence in Ruminant Abortion and Neonatal Mortality, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Afshari
A
A
Bahadori
H
H
Mohri
M
M
Seifi
H A
HA
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001774:Blood Chemical Analysis / Q000662:veterinary*; D001777:Blood Coagulation; D001786:Blood Glucose / Q000032:analysis; D002118:Calcium / Q000097:blood; D002417:Cattle / Q000097:blood*; D003402:Creatine Kinase / Q000097:blood; D003612:Dairying / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D013696:Temperature
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; D001663:Bilirubin; D002118:Calcium