title: [Matrine-induced erythroid differentiation of K562 cells is associated with activation of the apoptotic pathway].
abstract: OBJECTIVE
  To observe matrine-induced erythroid differentiation of K562 cells in relation to activation of the apoptotic pathway in vitro.
  
  
  METHODS
  K562 cells were cultured in the presence or absence of matrine at different concentrations for 4 days, and the morphological and ultramicrostructural changes of the cells were observed using inverted microscopy and transmission electron microscopy, respectively. The expression of apoptosis-related protein p27kip1 was detected by immunocytochemistry.
  
  
  RESULTS
  Compared to untreated K562 cells, the cells treated with matrine at 0.10 g/L exhibited apoptostic characteristics in the cellular morphology and ultramicrostructure, with the expression of p27kip1 protein upregulated in a concentration- and time-dependent manner.
  
  
  CONCLUSIONS
  Matrine-induced erythroid differentiation of K562 cells is associated with cell apoptosis, and upregulation of p27kip1 protein expression may play a crucial role in the process of apoptosis.
authors: Department of Child Health Care, Zhongshan Bo'ai Hospital, Southern Medical University, Zhongshan 528403, China. zhangcuimei0527@163.com
authors: Cui-mei
authors: CM
authors: Zhang
authors: Jian-hui
authors: JH
authors: Gao
authors: De-le
authors: DL
authors: Li
authors: Jing
authors: J
authors: Li
authors: Yu-qi
authors: YQ
authors: Shi
authors: Jun
authors: J
authors: Lin
authors: Shen-qiu
authors: SQ
authors: Luo
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology; D046529:Microscopy, Electron, Transmission; D011807:Quinolizines / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D050760:Cyclin-Dependent Kinase Inhibitor p27; D000093842:Matrines