Molecular recognition of endocrine disruptors by synthetic and natural 17beta-estradiol receptors: a comparative study.
A beta-estradiol receptor binding mimic was synthesised using molecular imprinting. Bulk polymers and spherical polymer nanoparticles based on methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and crosslinker, respectively, were prepared in acetonitrile. The selectivity was evaluated by radioligand binding assays. The imprinted polymers were very specific to beta-estradiol since the control polymers bound virtually none of the radioligand. The bulk polymer was then employed to screen endocrine disrupting chemicals. Structurally related steroids like alpha-estradiol, estrone and ethynylestradiol showed, respectively, 14.0, 5.0 and 0.7% of relative binding to the beta-estradiol polymer, whereas most unrelated chemicals did not bind at all. These results are compared to those obtained with a bioassay using stably transfected yeast cells in culture bearing the human estrogen receptor. The receptor was activated by several estrogen-like chemicals and to a lesser extent by some structurally related chemicals. [figure: see text]
Tse Sum Bui
Bernadette
B
Compiègne University of Technology, UMR CNRS 6022, B.P. 20529, 60205, Compiégne cedex, France.
Belmont
Anne-Sophie
AS
Witters
Hilda
H
Haupt
Karsten
K
D001681:Biological Assay / Q000379:methods; D052244:Endocrine Disruptors / Q000032:analysis*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D008968:Molecular Conformation; D054802:Molecular Imprinting / Q000379:methods*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011959:Receptors, Estradiol / Q000737:chemistry*; D011960:Receptors, Estrogen / Q000737:chemistry
D052244:Endocrine Disruptors; D008689:Methacrylates; D011108:Polymers; D011959:Receptors, Estradiol; D011960:Receptors, Estrogen; C008384:methacrylic acid; C004919:ethylene dimethacrylate