title: Patterned growth of neuronal cells on modified diamond-like carbon substrates.
abstract: Diamond-like carbon (DLC) has been explored as a biomaterial with potential use for coating implantable devices and surgical instruments. In this study the interaction of DLC with mammalian neuronal cells has been studied along with its modifications to improve its function as a biomaterial. We describe the use of DLC, oxidised DLC and phosphorus-doped DLC to support the growth and survival of primary central nervous system neurones and neuroblastoma cells. None of these substrates were cytotoxic and primary neurones adhered better to phosphorus-doped DLC than unmodified DLC. This property was used to culture cortical neurones in a predetermined micropattern. This raises the potential of DLC as a biomaterial for central nervous system (CNS) implantation. Furthermore, patterned DLC and phosphorus-doped DLC can direct neuronal growth, generating a powerful tool to study neuronal networks in a spatially distinct way. This study reports the generation of nerve cell patterns via patterned deposition of DLC.
authors: Henry Wellcome L.I.N.E., Clinical Sciences South Bristol, University of Bristol, UK.
authors: Stephen
authors: S
authors: Kelly
authors: Edward M
authors: EM
authors: Regan
authors: James B
authors: JB
authors: Uney
authors: Andrew D
authors: AD
authors: Dick
authors: Joseph P
authors: JP
authors: McGeehan
authors: Eric J
authors: EJ
authors: Mayer
authors: Frederik
authors: F
authors: Claeyssens
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002244:Carbon; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D018130:Diamond; D004622:Embryo, Mammalian / Q000166:cytology; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000166:cytology* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010758:Phosphorus / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013059:Spectrum Analysis, Raman; D013379:Substrate Specificity; D013778:Tetrazolium Salts / Q000032:analysis; D013844:Thiazoles / Q000032:analysis
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D013778:Tetrazolium Salts; D013844:Thiazoles; D011107:Polylysine; D010758:Phosphorus; D002244:Carbon; D018130:Diamond; C022616:thiazolyl blue