title: Quantitative determination of glyphosate in human serum by 1H NMR spectroscopy.
abstract: The determination and quantification of glyphosate in serum using (1)H NMR spectroscopy is reported. This method permitted serum samples to be analysed without derivatization or any other sample pre-treatment, using 3-trimethylsilyl 2,2',3,3'-tetradeuteropropionic acid (TSP-d(4)) as a qualitative and quantitative standard. Characterization of the herbicide N-(phosphonomethyl)glycine was performed by analysing chemical shifts and coupling constant patterns. Quantification was performed by relative integration of CH(2)-P protons to the TSP-d(4) resonance peak. The method was tested for repeatability (n=5) and yielded coefficients of variation of 1% and 3%, respectively: detection and quantification limits were also determined and were 0.03 and 0.1mmol/L, respectively. The method was applied to the quantification of glyphosate in a case of acute poisoning.
authors: Laboratoire de Toxicologie et Génopathies, Hôpital Calmette, 59045 Lille Cedex, France.
authors: Bernard
authors: B
authors: Cartigny
authors: Nathalie
authors: N
authors: Azaroual
authors: Michel
authors: M
authors: Imbenotte
authors: Daniel
authors: D
authors: Mathieu
authors: Erika
authors: E
authors: Parmentier
authors: Gaston
authors: G
authors: Vermeersch
authors: Michel
authors: M
authors: Lhermitte
mesh_terms: D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D012680:Sensitivity and Specificity; D000097797:Glyphosate
chemical_list: D005998:Glycine; D011522:Protons; D000097797:Glyphosate