PEG-ylated cationic CdSe/ZnS QDs as an efficient intracellular labeling agent.
Quantum dots (QDs) have size-tunable optical properties, such as photostability, strong photoluminescence and a large Stokes-shift, which make it possible to adapt them to various biological applications. In many cases, surface modification of QDs by carboxylate ligands has been extensively studied. However, there have been few applications on QDs modified with a potential cationic ligand such as amine. In this study, we synthesized robust amine-functionalized QDs and modified their surface with poly(ethylene glycol) for long-term stability. These QDs showed an excellent stability over a broad pH-range and remarkable internalization efficiency into living cells.
Lee
Junghan
J
Nano/Bio Chemistry Laboratory, Institut Pasteur Korea (IP-K), 39-1 Hawolgok-dong Seongbuk-gu, 136-791, Seoul, Korea.
Kim
Junwon
J
Park
Eunjung
E
Jo
Shineun
S
Song
Rita
R
D002264:Carboxylic Acids / Q000737:chemistry; D002412:Cations; D003584:Cytological Techniques; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D008956:Models, Chemical; D015336:Molecular Probe Techniques; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D045663:Quantum Dots
D002264:Carboxylic Acids; D002412:Cations; D008024:Ligands; D010455:Peptides; D011092:Polyethylene Glycols