Dual examinations for identification of urine as being of human origin and for DNA-typing from small stains of human urine.
Concurrent methods for identification of urine as being of human origin, and for DNA-typing from small stains of human urine were examined. A urine stain was extracted with phosphate-buffered saline (PBS), and the extract was filtered using a Centricon-100 device. The filtrate was subjected to electrospray ionization liquid chromatography-mass spectrometry (ESI-LC-MS) for identification of human urine and a DNA-typing sample was obtained by dialfiltration of the residue using a DNA purification kit. After the purified residue was treated with an AmpflSTR Profiler PCR amplification kit, the DNA-types were analyzed by capillary electrophoresis using a Genetic Analyzer. It was possible to identify a urine stain as being of human origin, and complete DNA profiles could be successfully obtained from a urine stain which had been created by 50 microL of female urine. Serial analyses of urine stains found at a crime scene provide effective information for forensic investigation. This method is recommended for stain identification and for DNA-typing from a urine stain.
Nakazono
Takehiko
T
Forensic Science Laboratory, Fukuoka Prefectural Police Headquarters, Fukuoka, Japan. nakazono@indigo.plala.or.jp
Kashimura
Seiich
S
Hayashiba
Yasuhiko
Y
Hara
Kenji
K
Matsusue
Aya
A
Augustin
Christa
C
D015068:17-Ketosteroids / Q000652:urine; D004247:DNA / Q000652:urine*; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D016133:Polymerase Chain Reaction; D021241:Spectrometry, Mass, Electrospray Ionization
D015068:17-Ketosteroids; D004247:DNA