[Preliminary study of BMSCs seeded into collagen I-glycosaminoglycan matrices induced toward cartilage].
OBJECTIVE
To investigate the possibility of BMSCs seeded into collagen I -glycosaminoglycan (CG) matrices to form the tissue engineered cartilage through chondrocyte inducing culture.


METHODS
Bone marrow aspirate of dogs was cultured and expanded to the 3rd passage. BMSCs were harvested and seeded into the dehydrothemal treatment (DHT) cross-linked CG matrices at 1 x 10(6) cells per 9 mm diameter sample. The samples were divided into experimental group and control group. In the experimental group, chondrogenic differentiation was achieved by the induction media for 2 weeks. Medium was changed every other day in both experimental group and control group. The formation of cartilage was assessed by HE staining and collagen II immunohistochemical staining.


RESULTS
The examinations under the inverted phase contrast microscope indicated the 2nd and 3rd passage BMSCs had the similar morphology. HE staining showed the BMSCs in the experimental group appeared polygon or irregular morphology in the CG matrices, while BMSCs in the control group appeared fibroblast-like spindle or round morphology in the CG matrices. Extracellular matrix could be found around cells in the experimental group. Two weeks after seeded, the cells grew in the CG matrices, and positive collagen II staining appeared around the cells in the experimental group. There was no positive collagen II staining appeared in the control group.


CONCLUSIONS
It is demonstrated that BMSCs seeded CG matrices can be induced toward cartilage by induction media.
Hu
Wei
W
Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R. China.
Xiang
Zhou
Z
Zhang
Xueli
X
Zhou
Haitao
H
Yang
Zhiming
Z
D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D024042:Collagen Type I; D004285:Dogs; D005260:Female; D006025:Glycosaminoglycans; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D024042:Collagen Type I; D006025:Glycosaminoglycans