The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing.
Corynebacterium urealyticum is a lipid-requiring, urealytic bacterium of the human skin flora that has been recognized as causative agent of urinary tract infections. We report the analysis of the complete genome sequence of C. urealyticum DSM7109, which was initially recovered from a patient with alkaline-encrusted cystitis. The genome sequence was determined by a combination of pyrosequencing and Sanger technology. The chromosome of C. urealyticum DSM7109 has a size of 2,369,219bp and contains 2024 predicted coding sequences, of which 78% were considered as orthologous with genes in the Corynebacterium jeikeium K411 genome. Metabolic analysis of the lipid-requiring phenotype revealed the absence of a fatty acid synthase gene and the presence of a beta-oxidation pathway along with a large repertoire of auxillary genes for the degradation of exogenous fatty acids. A urease locus with the gene order ureABCEFGD may play a pivotal role in virulence of C. urealyticum by the alkalinization of human urine and the formation of struvite stones. Multidrug resistance of C. urealyticum DSM7109 is mediated by transposable elements, conferring resistances to macrolides, lincosamides, ketolides, aminoglycosides, chloramphenicol, and tetracycline. The complete genome sequence of C. urealyticum revealed a detailed picture of the lifestyle of this opportunistic human pathogen.
Tauch
Andreas
A
Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany. Andreas.Tauch@Genetik.Uni-Bielefeld.DE
Trost
Eva
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Tilker
Alexandra
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Ludewig
Ulrike
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Schneiker
Susanne
S
Goesmann
Alexander
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Arnold
Walter
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Bekel
Thomas
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Brinkrolf
Karina
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Brune
Iris
I
Götker
Susanne
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Kalinowski
Jörn
J
Kamp
Paul-Bertram
PB
Lobo
Francisco Pereira
FP
Viehoever
Prisca
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Weisshaar
Bernd
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Soriano
Francisco
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Dröge
Marcus
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Pühler
Alfred
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D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003352:Corynebacterium / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
D001426:Bacterial Proteins