title: Abnormal cellular reactivity to microbial antigens in patients with uveitis.
abstract: OBJECTIVE
  The purpose of the present study was to evaluate the cellular response to microbial antigens in patients with idiopathic uveitis.
  
  
  METHODS
  Blood lymphocytes from 31 patients with uveitis and 24 healthy controls were cultivated with microbial antigens and analyzed by flow cytometry after staining with monoclonal antibodies against CD3, CD4, and activation markers CD69 and CD25.
  
  
  RESULTS
  Although no difference was noted in circulating lymphocytes, the activation of T cells, detected with CD69, was higher in 24-hour blood culture from uveitis patients with Candida albicans antigen (Ca-Ag) than from controls, especially in posterior uveitis and panuveitis. Moreover, late response, detected with CD25, to different microbial antigens was higher in patient with uveitis.
  
  
  CONCLUSIONS
  Such results suggest the role of Ca-Ag and microbial antigens in the pathogenic mechanisms of idiopathic uveitis.
authors: Laboratoire d'Immunologie, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
authors: Grégoire Jacques Noël
authors: GJ
authors: Cozon
authors: Florentin Martial
authors: FM
authors: Mbitikon-Kobo
authors: Fathi
authors: F
authors: Fatoohi
authors: Myriam
authors: M
authors: Spire
authors: Jean-Daniel
authors: JD
authors: Grange
authors: Laurent
authors: L
authors: Kodjikian
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D014605:Uveitis / Q000276:immunology*; D000099192:CD69 Antigens
chemical_list: D000942:Antigens, Bacterial; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000946:Antigens, Fungal; D000953:Antigens, Protozoan; D017252:CD3 Complex; D037181:Lectins, C-Type; D000099192:CD69 Antigens