Biotransformation of acyclic monoterpenoids by Debaryomyces sp., Kluyveromyces sp., and Pichia sp. strains of environmental origin.
Sixty yeast strains, which belong to 32 species of the genera Debaryomyces, Kluyveromyces, and Pichia, and which were isolated from plant-, soil- or insect-associated habitats, were screened for their ability to biotransform the acyclic monoterpenes geraniol and nerol. The aptitude to convert both compounds (from 2.6 to 30.6, and from 2.7 to 29.1%/g cell DW (=dry weight), resp.) was apparently a broad distributed character in such yeasts. Depending upon the substrate used, the production of linalool, alpha-terpineol, beta-myrcene, D-limonene, (E)-beta-ocimene, (Z)-beta-ocimene, or carene was observed. Linalool was the main product obtained from geraniol, whereas linalool and alpha-terpineol were the main products obtained through the conversion of nerol. Yet, differently from nerol, the aptitude to exhibit high bioconversion yields of geraniol to linalool was an apparently genus-related character, whereas the ability to produce other monoterpenes was a both genus- and habitat-related character. The possible pathways of bioconversion of geraniol or nerol to their derivatives were proposed/discussed.
Ponzoni
Chiara
C
Dipartimento di Chimica, Università di Modena e Reggio Emilia, Via Campi 183, I-41100 Modena.
Gasparetti
Chiara
C
Goretti
Marta
M
Turchetti
Benedetta
B
Pagnoni
Ugo Maria
UM
Cramarossa
Maria Rita
MR
Forti
Luca
L
Buzzini
Pietro
P
D000080462:Acyclic Monoterpenes; D001711:Biotransformation; D007716:Kluyveromyces / Q000737:chemistry / Q000378:metabolism*; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism*; D010843:Pichia / Q000737:chemistry / Q000378:metabolism*; D004718:Saccharomycetales / Q000737:chemistry / Q000378:metabolism*; D013045:Species Specificity; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*
D000080462:Acyclic Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; C007836:geraniol