E2F1 contributes to the transcriptional activation of the KIR3DL1 gene.
The KIR3DL1 gene is a member of killer immunoglobulin-like receptors family, which exhibits a variegated expression pattern in NK cells and subsets of CD4(+) and CD8(+) T cells. The E2F family of transcription factors plays a crucial role in the regulation of gene expression. The present study reports a naturally occurring point mutation (TTTGGCGC-->TTCGGCGC) within a putative E2F binding site in the KIR3DL1 promoter in K562 cells. Interestingly, this mutation introduces a new methylation site. This study shows for the first time that E2F1 binds to the KIR3DL1 promoter in vivo. This point mutation and concomitantly altered methylation pattern within the E2F1 binding site abolishes their binding and reduces the promoter activity, while elevated expression of E2F1 correlates with increased promoter activity. Therefore, E2F1 contributes to the transcriptional activation of the KIR3DL1 gene.
Gao
Xiao-Ning
XN
Department of Hematology, General Hospital of PLA, 28 Fuxing Road, Beijing 100853, China.
Yu
Li
L
D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D019175:DNA Methylation; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D054341:Receptors, KIR3DL1 / Q000235:genetics*; D015533:Transcriptional Activation
D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; C515083:KIR3DL1 protein, human; D054341:Receptors, KIR3DL1