title: Hepatitis C virus NS5A anchor peptide disrupts human immunodeficiency virus.
abstract: In the absence of an effective vaccine, there is an urgent need for safe and effective antiviral agents to prevent transmission of HIV. Here, we report that an amphipathic alpha-helical peptide derived from the hepatitis C virus NS5A anchor domain (designated C5A in this article) that has been shown to be virocidal for the hepatitis C virus (HCV) also has potent antiviral activity against HIV. C5A exhibits a broad range of antiviral activity against HIV isolates, and it prevents infection of the three in vivo targets of HIV: CD4(+) T lymphocytes, macrophages, and dendritic cells by disrupting the integrity of the viral membrane and capsid core while preserving the integrity of host membranes. C5A can interrupt an ongoing T cell infection, and it can prevent transmigration of HIV through primary genital epithelial cells, infection of mucosal target cells and transfer from dendritic cells to T cells ex vivo, justifying future experiments to determine whether C5A can prevent HIV transmission in vivo.
authors: Departments of Immunology and Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors: Michael D
authors: MD
authors: Bobardt
authors: Guofeng
authors: G
authors: Cheng
authors: Lot
authors: L
authors: de Witte
authors: Suganya
authors: S
authors: Selvarajah
authors: Udayan
authors: U
authors: Chatterji
authors: Brigitte E
authors: BE
authors: Sanders-Beer
authors: Teunis B H
authors: TB
authors: Geijtenbeek
authors: Francis V
authors: FV
authors: Chisari
authors: Philippe A
authors: PA
authors: Gallay
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology; D006678:HIV / Q000187:drug effects*; D015658:HIV Infections / Q000517:prevention & control*; D016174:Hepacivirus / Q000737:chemistry*; D006801:Humans; D008264:Macrophages / Q000821:virology; D010446:Peptide Fragments / Q000494:pharmacology* / Q000627:therapeutic use; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012324:RNA-Dependent RNA Polymerase
chemical_list: D010446:Peptide Fragments; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase