An anti-c-Fms antibody inhibits orthodontic tooth movement.
Orthodontic force induces osteoclastogenesis in vivo. It has recently been reported that administration of an antibody against the macrophage-colony-stimulating factor (M-CSF) receptor c-Fms blocks osteoclastogenesis and bone erosion induced by tumor necrosis factor-alpha (TNF-alpha) administration. This study aimed to examine the effect of an anti-c-Fms antibody on mechanical loading-induced osteoclastogenesis and osteolysis in an orthodontic tooth movement model in mice. Using TNF receptor 1- and 2-deficient mice, we showed that orthodontic tooth movement was mediated by TNF-alpha. We injected anti-c-Fms antibody daily into a local site, for 12 days, during mechanical loading. The anti-c-Fms antibody significantly inhibited orthodontic tooth movement, markedly reduced the number of osteoclasts in vivo, and inhibited TNF-alpha-induced osteoclastogenesis in vitro. These findings suggest that M-CSF plays an important role in mechanical loading-induced osteoclastogenesis and bone resorption during orthodontic tooth movement mediated by TNF-alpha.
Kitaura
H
H
Divisions of Orthodontic and Dentofacial Orthopedics, Department of Translational Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8588, Japan. kitaura@nagasaki-u.ac.jp
Yoshimatsu
M
M
Fujimura
Y
Y
Eguchi
T
T
Kohara
H
H
Yamaguchi
A
A
Yoshida
N
N
D000135:Acid Phosphatase / Q000037:antagonists & inhibitors; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D001862:Bone Resorption / Q000503:physiopathology; D002454:Cell Differentiation / Q000187:drug effects; D007074:Immunoglobulin G / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D023421:Models, Animal; D010010:Osteoclasts / Q000187:drug effects; D010014:Osteolysis / Q000503:physiopathology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000037:antagonists & inhibitors; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000037:antagonists & inhibitors; D013314:Stress, Mechanical; D000071681:Tartrate-Resistant Acid Phosphatase; D014087:Tooth Movement Techniques; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007074:Immunoglobulin G; D007527:Isoenzymes; D047888:Receptors, Tumor Necrosis Factor, Type I; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; D016186:Receptor, Macrophage Colony-Stimulating Factor; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase