title: Quality control of medicinal herbs Fructus gardeniae, Common Andrographis Herb and their preparations for their active constituents by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry.
abstract: Dehydroandrographolide, andrographolide and geniposide are the main active constituents of many herbal medicines, e.g., Fructus gardeniae, Common Andrographis Herb. They are used as the markers to control the quality of such herbal medicines and their herbal preparations. In this paper, a simple and sensitive high-performance liquid chromatographic (HPLC) method coupled with photodiode array detection (DAD) and electrospray mass spectrometry (ESI/MS) were developed to determine the three compounds simultaneously in extracts of medicinal herbs and herbal preparations produced by different companies. The extracts were separated on a C(18) reversed phase HPLC column, with a gradient solvent system, the time for the separation of the three target analytes was 1 0min. The abundance ions were recorded using selected ion monitoring (SIM) mode with m/z 297.3, 297.3 and 411.1 for dehydroandrographolide, andrographolide and geniposide, respectively. The limit of detection for dehydroandrographolide, andrographolide and geniposide were 20, 30 and 150 ng mL(-1), respectively. The proposed method was successfully applied to the determination of the contents of the compounds in related to medicinal herbs and preparations.
authors: College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors: Li
authors: L
authors: Ding
authors: Xu Biao
authors: XB
authors: Luo
authors: Fei
authors: F
authors: Tang
authors: Jin Bin
authors: JB
authors: Yuan
authors: Manli
authors: M
authors: Guo
authors: Shou Zhuo
authors: SZ
authors: Yao
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000032:analysis; D039823:Iridoids / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards
chemical_list: D004224:Diterpenes; D039823:Iridoids; D010936:Plant Extracts; D011714:Pyrans; C007835:geniposide; C030419:andrographolide