Environmentally relevant concentrations of pharmaceuticals influence the initial adhesion of bacteria.
Pharmaceuticals are abundant at low concentrations (i.e. ng L(-1); microg L(-1)) in natural aquatic systems. However, very little is known about their effects on microorganisms. This study investigated the influence of the pharmaceuticals phenazone, amoxicillin and erythromycin, at low, non-toxic concentrations (i.e. 0.5-50 microg L(-1)), on the initial adhesion of bacteria to uncoated and iron-coated polystyrene. The influence of the pharmaceuticals on the initial adhesion of bacterial pure cultures (Escherichia coli, Aquabacterium commune, Bacillus subtilis) isolated from natural aquatic systems, was investigated with a plate assay. Initial adhesion of the pure cultures depended on the selected pharmaceutical, its concentration, the bacterial strain and the adhesion surface. Different combinations of these parameters resulted in inhibition, enhancement or had no effect on initial adhesion. In addition, a continuous flow system was used to investigate the influence of the pharmaceuticals on the initial adhesion of a drinking water microbial community. The drinking water community showed decreased adhesion in the presence of the pharmaceuticals regardless of adhesion surface. The results show that pharmaceuticals at environmentally relevant concentrations can influence the initial adhesion of bacteria. Thus, pharmaceutical compounds that are introduced to natural aquatic systems are able to exert subtle effects on bacteria.
Schreiber
Frank
F
Department of Environmental Microbiology, Technical University Berlin, Franklin Str. 29, 10587 Berlin, Germany. fschreib@mpi-bremen.de
Szewzyk
Ulrich
U
D000658:Amoxicillin / Q000633:toxicity*; D000900:Anti-Bacterial Agents / Q000633:toxicity; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D000983:Antipyrine / Q000633:toxicity*; D001412:Bacillus subtilis / Q000187:drug effects / Q000502:physiology; D001422:Bacterial Adhesion / Q000187:drug effects*; D020562:Betaproteobacteria / Q000187:drug effects / Q000502:physiology; D004917:Erythromycin / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D005290:Ferric Compounds / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D014881:Water Supply
D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005290:Ferric Compounds; D011137:Polystyrenes; D014874:Water Pollutants, Chemical; C000499:ferric oxide; D004917:Erythromycin; D000658:Amoxicillin; D000983:Antipyrine