Expression of interferon-stimulated gene 20 in vascular endothelial cells.
ISG20 is an ribonuclease specific for single-stranded RNA and considered to play a role in innate immunity against virus infections. We herein show that both poly IC, an authentic double-stranded RNA, and IFN-gamma induced ISG20 expression in cultured HUVEC. Poly IC-induced ISG20 expression was inhibited by LY294002, an inhibitor of PI3K, or by RNA interference against IFN regulatory factor three. ISG20 expression was not induced by IFN-beta, loxoribine or CpG oligonucleotide. These results suggest that ISG20 induction by poly IC may not be dependent on the IRF-3-mediated type I IFN induction pathway in HUVEC. ISG20 may be involved in innate immunity against viral infection in vascular endothelial cells.
Imaizumi
Tadaatsu
T
Department of Vascular Biology, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan. timaizum@cc.hirosaki-u.ac.jp
Mechti
Nadir
N
Matsumiya
Tomoh
T
Sakaki
Hirotaka
H
Kubota
Kosei
K
Yoshida
Hidemi
H
Kimura
Hiroto
H
Satoh
Kei
K
D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology; D042783:Endothelial Cells / Q000276:immunology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005092:Exonucleases / Q000096:biosynthesis*; D005095:Exoribonucleases; D020869:Gene Expression Profiling; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D016899:Interferon-beta / Q000494:pharmacology; D007372:Interferons / Q000276:immunology*; D009025:Morpholines / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D011070:Poly I-C / Q000276:immunology
C408982:CPG-oligonucleotide; D002867:Chromones; D004791:Enzyme Inhibitors; D007155:Immunologic Factors; D009025:Morpholines; D009838:Oligodeoxyribonucleotides; D006151:Guanosine; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D016899:Interferon-beta; D007372:Interferons; C068562:loxoribine; D005092:Exonucleases; D005095:Exoribonucleases; C112346:ISG20 protein, human; D011070:Poly I-C