Invasion of metastatic human follicular thyroid cancer is inhibited via antagonism of protein kinase C.
Signal transduction of a human follicular thyroid cancer cell line (FTC133) was investigated. The protein kinase C (PKC)-agonist TPA enhanced invasion by 15%, whereas its antagonists staurosporine, chelerythrine and calphostin C were inhibiting by up to 62%. TSH and EGF stimulated invasion of FTC133. Antagonism of PKC reversed TSH-mediated stimulation, whereas it had no effect on EGF-stimulation. Our data provide evidence for an essential role of PKC in signal transduction of invasive thyroid cancer.
Hoelting
T
T
Department of Surgery, University of Heidelberg, Im Neuenheimer Feld 110, 69120 Heidelberg, Germany.
Duh
Q Y
QY
Clark
O H
OH
Herfarth
C
C
D053119:Benzophenanthridines / Q000494:pharmacology; D045744:Cell Line, Tumor; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D009281:Naphthalenes / Q000494:pharmacology; D009361:Neoplasm Invasiveness; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D019311:Staurosporine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013964:Thyroid Neoplasms / Q000201:enzymology / Q000473:pathology*; D013972:Thyrotropin / Q000494:pharmacology
D053119:Benzophenanthridines; D004791:Enzyme Inhibitors; D009281:Naphthalenes; D013972:Thyrotropin; C016299:chelerythrine; D011493:Protein Kinase C; D019311:Staurosporine; C058819:calphostin C; D013755:Tetradecanoylphorbol Acetate