title: Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction.
abstract: Six molecularly imprinted polymers (MIPs) of erythromycin (ERY) were prepared by noncovalent bulk polymerization using methacrylic acid (MAA) as the functional monomer. On the basis of binding analysis, the MIPs with 1:2 optimum ratio of template to MAA were selected for subsequent scanning electron microscopy and Brunauer-Emmett-Teller analyses, which indicated that the MIPs had more convergent porous structures than the nonimprinted polymers. The equilibrium binding experiments showed that the binding sites of MIPs were heterogeneous, with two dissociation constants of 0.005 and 0.63 mg mL(-1), respectively. Furthermore, the performance of the MIPs as solid-phase extraction (SPE) sorbents was evaluated, and the selectivity analysis showed that the MIPs could recognize ERY with moderate cross-reactivity for other macrolides. The overall investigation of molecularly imprinted SPE for cleanup and enrichment of the ERY in pig muscle and tap water confirmed the feasibility of utilizing the MIPs obtained as specific SPE sorbents for ERY extraction in real samples. [figure: see text]
authors: Bor Luh Food Safety Center, Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
authors: Suquan
authors: S
authors: Song
authors: Aibo
authors: A
authors: Wu
authors: Xizhi
authors: X
authors: Shi
authors: Rongxiu
authors: R
authors: Li
authors: Zhixin
authors: Z
authors: Lin
authors: Dabing
authors: D
authors: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D001665:Binding Sites; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004917:Erythromycin / Q000032:analysis* / Q000493:pharmacokinetics; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D015394:Molecular Structure; D009130:Muscle, Smooth / Q000737:chemistry; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine; D014874:Water Pollutants, Chemical / Q000032:analysis; D014881:Water Supply / Q000032:analysis
chemical_list: D008689:Methacrylates; D011108:Polymers; D014874:Water Pollutants, Chemical; C008384:methacrylic acid; D004917:Erythromycin