[The disinfecting effect of ozone on four kinds of bacteria].
OBJECTIVE
To evaluate the disinfecting effect of ozone on 4 kinds of bacteria: Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis and Streptococcus pneumoniae.


METHODS
The concentration of ozone that was transmitted by ozone generator at different time was determined by using iodine titrimetric method. According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50 microl eluant was seeded on the TSA plates. The TSA plates were put into the anaerobiotic incubator (90% N(2),10% CO(2),37 degrees centigrade). After 24 to 48 hours, the CFU on the plate was counted.The data was analyzed by one-way ANOVA with SAS 6.12 software package.


RESULTS
The sterilization effect depended on the ozone concentration and the treatment time. When the 4 kinds of bacteria were treated with 2.73 mg/L ozone for 45 minutes, there was no bacteria alive.


CONCLUSIONS
Ozone has obvious disinfecting effect on the 4 kinds of bacteria and the effect is correlated with the concentration of ozone.
Wang
Rao-Rao
RR
Department of Dentistry, The 10th People's Hospital of Shanghai, Shanghai 200072, China.
Shang
Guang-Wei
GW
Hu
Lin-Feng
LF
Wang
Xiao-Ping
XP
Huang
Wen
W
Li
Ming-Yu
MY
Zhu
Cai-Lian
CL
D000900:Anti-Bacterial Agents; D004202:Disinfectants; D010126:Ozone; D013211:Staphylococcus aureus; D013295:Streptococcus mutans
D000900:Anti-Bacterial Agents; D004202:Disinfectants; D010126:Ozone