title: Bioactive glass induced in vitro apatite formation on composite GBR membranes.
abstract: The aim of this study was to investigate in vitro bioactivity of different thermoplastic biodegradable barrier membranes. Three experimental GBR membranes were fabricated using Poly(epsilon-caprolactone-co-D: ,L-lactide) P(CL/DL-LA) and particulate bioactive glass S53P4 (BAG; granule size 90-315 microm): (A) composite membrane with 60-wt.% of BAG, (B) membrane coated with BAG; and (C) copolymer membrane without BAG. Membranes were immersed in simulated body fluid (SBF), and their surfaces were characterized with SEM, XRD and EDS after 6 and 12 h and after 1, 3, 5, 7, and 14 days. Calcium phosphate (Ca-P) surface formation was observed on both composite membranes (A and B) but not on the copolymer membrane without bioactive glass (C). The Ca-P precipitation appeared to be initiated on the bioactive glass followed by growth of the layer along the polymer surface. In 6-12 h ion dissolution of the bioactive glass led to formation of the silica rich layer on the surface of the exposed glass granules on composite membrane B whereas only small amounts of silica was observed on the polymer surface of the composite membrane A. At 24 h nucleation of Ca-P precipitation was observed, and by 3-5 days membrane surface was covered with a uniform Ca-P layer transforming from amorphous to low crystalline structure. At 7 days composition and structure of the apatite surface resembled the apatite in bone. Once nucleated, the surface topography seemed to have significant effect on the growth of the apatite layer.
authors: Department of Prosthetic Dentistry and Biomaterials Science, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, 20520 Turku, Finland. teemu.tirri@utu.fi
authors: Teemu
authors: T
authors: Tirri
authors: Jaana
authors: J
authors: Rich
authors: Joop
authors: J
authors: Wolke
authors: Jukka
authors: J
authors: Seppälä
authors: Antti
authors: A
authors: Yli-Urpo
authors: Timo O
authors: TO
authors: Närhi
mesh_terms: D001031:Apatites / Q000138:chemical synthesis*; D005898:Glass; D048091:Guided Tissue Regeneration; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites