Regulatory involvement of abscisic acid in potato tuber wound-healing.
Rapid wound-healing is crucial in protecting potato tubers from infection and dehydration. Wound-induced suberization and the accumulation of hydrophobic barriers to reduce water vapour conductance/loss are principal protective wound-healing processes. However, little is known about the cognate mechanisms that effect or regulate these processes. The objective of this research was to determine the involvement of abscisic acid (ABA) in the regulation of wound-induced suberization and tuber water vapour loss (dehydration). Analysis by liquid chromatography-mass spectrometry showed that ABA concentrations varied little throughout the tuber, but were slightly higher near the periderm and lowest in the pith. ABA concentrations increase then decrease during tuber storage. Tuber wounding induced changes in ABA content. ABA content in wound-healing tuber discs decreased after wounding, reached a minimum by 24 h, and then increased from the 3rd to the 7th day after wounding. Wound-induced ABA accumulations were reduced by fluridone (FLD); an inhibitor of de novo ABA biosynthesis. Wound-induced phenylalanine ammonia lyase activity was slightly reduced and the accumulation of suberin poly(phenolics) and poly(aliphatics) noticeably reduced in FLD-treated tissues. Addition of ABA to the FLD treatment restored phenylalanine ammonia lyase activity and suberization, unequivocally indicating that endogenous ABA is involved in the regulation of these wound-healing processes. Similar experiments showed that endogenous ABA is involved in the regulation of water vapour loss, a process linked to wax accumulation in wound-healing tubers. Rapid reduction of water vapour loss across the wound surface is essential in preventing desiccation and death of cells at the wound site; live cells are required for suberization. These results unequivocally show that endogenous ABA is involved in the regulation of wound-induced suberization and the processes that protect surface cells from water vapour loss and death by dehydration.
Lulai
Edward C
EC
USDA-ARS, Northern Crop Science Laboratory, 1307 18th Street North, Fargo, ND 58105, USA. ed.lulai@ars.usda.gov
Suttle
Jeffrey C
JC
Pederson
Shana M
SM
D000040:Abscisic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010937:Plant Growth Regulators / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018526:Plant Transpiration / Q000187:drug effects; D035281:Plant Tubers / Q000166:cytology / Q000502:physiology*; D011728:Pyridones / Q000494:pharmacology; D011198:Solanum tuberosum / Q000502:physiology*
D010937:Plant Growth Regulators; D010940:Plant Proteins; D011728:Pyridones; C013351:fluridone; D000040:Abscisic Acid; D010650:Phenylalanine Ammonia-Lyase