Hapten synthesis and monoclonal antibody-based immunoassay development for detection of the fungicide trifloxystrobin.
High-affinity and selective monoclonal antibodies have been produced against the strobilurin fungicide trifloxystrobin. A battery of functionalized haptens has been synthesized, and conjugate-coated enzyme-linked immunosorbent assays following different procedures have been developed. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.10 ng/mL for the extended assay and 0.17 ng/mL for the rapid assay. On the other hand, an immunoassay in the conjugate-coated format was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.21 ng/mL. All of these assays showed detection limits for trifloxystrobin in the low parts per billion range, well below the common maximum residue limits for this pesticide in foodstuffs (50 microg/kg).
Mercader
Josep V
JV
Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, 46100 Burjassot, València, Spain.
Suárez-Pantaleón
Celia
C
Agulló
Consuelo
C
Abad-Somovilla
Antonio
A
Abad-Fuentes
Antonio
A
D000085:Acetates / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005659:Fungicides, Industrial / Q000032:analysis*; D006241:Haptens / Q000737:chemistry* / Q000276:immunology; D007097:Imines / Q000032:analysis* / Q000276:immunology; D008689:Methacrylates / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D000073739:Strobilurins
D000085:Acetates; D000911:Antibodies, Monoclonal; D005659:Fungicides, Industrial; D006241:Haptens; D007097:Imines; D008689:Methacrylates; D010573:Pesticide Residues; D000073739:Strobilurins; C467051:trifloxystrobin