Nuclear ferritin-mediated protection of corneal epithelial cells from oxidative damage to DNA.
We previously obtained evidence that ferritin is a nuclear protein in embryonic avian corneal epithelial (CE) cells, and that the ferritin in this site protects DNA from UV-induced damage. UV irradiation is known to produce reactive oxygen species (ROS) and ferritin is known to ameliorate further oxidative damage by sequestering free iron, thus decreasing the formation of hydroxyl radicals through the Fenton reaction. Here we present evidence that nuclear ferritin can similarly prevent damage by the ROS, H2O2. These results, when coupled with our previous ones showing that nuclear ferritin appears in the CE early in its development, raises the possibility that this ferritin may serve two protective roles. The initial one would be during embryonic development to protect the CE from ROS endogenously produced by the embryo itself (e.g., H2O2; the latter one would be post-hatching to protect the CE from environmentally produced oxidative insults (e.g., from U.V. light).
Cai
Cindy
C
St. Louis University School of Medicine, St. Louis, Missouri, USA.
Ching
Anna
A
Lagace
Christopher
C
Linsenmayer
Thomas
T
D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D003315:Cornea / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism*; D005293:Ferritins / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D005293:Ferritins; D006861:Hydrogen Peroxide; D007501:Iron