[A novel retinoid CD437 induces apoptosis of human epidermoid carcinoma A431 cells].
OBJECTIVE
To investigate the effect of a novel retinoid CD437 and all-trans retinoic acid (ATRA) in inducing cell apoptosis and inhibiting the proliferation of human epidermoid carcinoma A431 cells and normal human epidermal keratinocytes.


METHODS
MTT assay was used to determine the inhibitory effects of CD437 and ATRA on the growth of A431 cells and normal human epidermal keratinocytes, and the cell morphological changes were observed microscopically. Flow cytometry was used to investigate the effect of CD437 and ATRA on the cell cycle and apoptosis.


RESULTS
CD437 was more effective than ATRA in inhibiting the proliferation of A431 cells and normal human epidermal keratinocytes. CD437 increased the percentage of sub-G1 populations in A431 cells and induced G1 arrest in normal human epidermal keratinocytes. ATRA appeared to be relatively ineffective for inducing apoptosis in A431 cells as compared to CD437. CD437 did not duce obvious apoptosis in normal human epidermal keratinocytes.


CONCLUSIONS
CD437 is more effective than ATRA in inhibiting the proliferation and inducing apoptosis in A431 cells and shows selective apoptosis-inducing effect against malignant keratinocytes, suggesting its potential in the prevention or treatment of cutaneous carcinoma.
Pan
Min
M
Department of Dermatology, Second Hospital Affiliated to Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. pan730412@126.com
Peng
Zhen-hui
ZH
Xiao
Sheng-xiang
SX
Li
Xiao-li
XL
Liu
Yan
Y
Li
Zheng-xiao
ZX
Ren
Jian-wen
JW
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004817:Epidermis / Q000187:drug effects / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D012176:Retinoids / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D055815:Young Adult
D000970:Antineoplastic Agents; C099555:CD 437; D012176:Retinoids; D014212:Tretinoin