title: Identification of nickel chelators in three hyperaccumulating plants: an X-ray spectroscopic study.
abstract: We have investigated the accumulation of nickel in a hyperaccumulating plant from the Brassicacae family Leptoplax emarginata (Boiss.) O.E. Schulz. Two supplementary hyperaccumulating plants, which have been the subject of a high number of publications, Alyssum murale Waldst. & Kit and Thlaspi caerulescens J.&C. Presl, and a nonaccumulating species Aurinia saxatilis were also studied for reference. The plants were grown during 4 months in specific rhizoboxes with Ni-bearing minerals as a source of nickel. Nickel speciation was analyzed through X-ray absorption spectroscopy at Ni K-edge (X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure spectroscopy) in the different parts of the plants (leaves, stems and roots) and compared with aqueous solutions containing different organo-Ni(II) complexes. Carboxylic acids (citrate, malate) appeared as the main ligands responsible of nickel transfer within those plants. Citrate was found as the predominant ligand for Ni in stems of Leptoplax and Alyssum, whereas in leaves of the three plants, malate appeared as the chelating organic acid of accumulated metal. Histidine could not be detected either in leaves, stems nor roots of any studied plant sample.
authors: Laboratoire Environnement et Minéralurgie, Nancy Université, CNRS, 15 Avenue du Charmois, BP 40, 54501 Vandoeuvre les Nancy Cedex, France. Emmanuelle.montarges@ensg.inpl-nancy.fr
authors: Emmanuelle
authors: E
authors: Montargès-Pelletier
authors: Vanessa
authors: V
authors: Chardot
authors: Guillaume
authors: G
authors: Echevarria
authors: Laurent J
authors: LJ
authors: Michot
authors: Allan
authors: A
authors: Bauer
authors: Jean-Louis
authors: JL
authors: Morel
mesh_terms: D019607:Brassicaceae / Q000737:chemistry* / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry* / Q000378:metabolism; D019343:Citric Acid / Q000032:analysis / Q000737:chemistry; D018698:Glutamic Acid / Q000032:analysis / Q000737:chemistry; D008024:Ligands; D008293:Malates / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry* / Q000378:metabolism; D013053:Spectrophotometry; D031227:Thlaspi / Q000737:chemistry* / Q000378:metabolism; D014965:X-Rays
chemical_list: D002614:Chelating Agents; D008024:Ligands; D008293:Malates; D019343:Citric Acid; D018698:Glutamic Acid; D009532:Nickel; C030298:malic acid