A simple silica-based method for metagenomic DNA extraction from soil and sediments.
A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.
Rojas-Herrera
R
R
Universidad Autónoma de Yucatán, Ciudad Industrial, Mérida, Yucatán, Mexico. r.rojas@uady.mx
Narváez-Zapata
J
J
Zamudio-Maya
M
M
Mena-Martínez
M E
ME
D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D023281:Genomics; D019015:Geologic Sediments / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*; D012987:Soil / Q000032:analysis*
D017931:DNA Primers; D012987:Soil; D012822:Silicon Dioxide; D004247:DNA