[Effect of berberine hydrochloride on the expression of interleukin-1beta and tumor necrosis factor-alpha in periodontal tissues in rats].
OBJECTIVE
To determine the effects of berberine hydrochloride on the expressions of interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) in periodontal tissues in rat periodontitis model.


METHODS
Based on the successful rat periodontitis model, the experimental rats were randomized into different groups followed by oral treatment with berberine hydrochloride for 1, 2, 3, 4 weeks and then the rats were sacrificed and analyzed. Pathological assay and HE staining were used to detect the general conditions and pathological changes of rat periodontal tissues. And immunohistochemical staining was conducted to determine the expressions of IL-1beta and TNF-alpha in rats periodontitis model periodontal tissues.


RESULTS
The levels of IL-1beta and TNF-alpha in the periodontitis tissues were significantly higher than that in the control group. Treatment with berberine hydrochloride decreased the levels of IL-1beta and TNF-alpha in periodontitis tissues (P<0.05). Moreover, the general conditions and pathological changes in the control group and groups treated with berberine hydrochloride were much better than that in periodontitis groups.


CONCLUSIONS
Berberine hydrochloride inhibited the expression of IL-1beta and TNF-alpha in periodontal tissues in rats periodontitis model and promoted the regeneration of the periodontal tissues. This study suggested that berberine hydrochloride may have potential clinical application.
Yu
Zhan-hai
ZH
Department of Prosthodontics, College of Stomatology, Lanzhou University, Lanzhou 730000, China.
Zhang
Guo-ying
GY
Zhang
Xiao-heng
XH
Du
Jian-dong
JD
Yang
Qian
Q
He
Fu-de
FD
D000818:Animals; D001599:Berberine; D053583:Interleukin-1beta; D008297:Male; D010518:Periodontitis; D051381:Rats; D014409:Tumor Necrosis Factor-alpha
D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D001599:Berberine