[Matrine-induced erythroid differentiation of K562 cells is associated with activation of the apoptotic pathway].
OBJECTIVE
To observe matrine-induced erythroid differentiation of K562 cells in relation to activation of the apoptotic pathway in vitro.


METHODS
K562 cells were cultured in the presence or absence of matrine at different concentrations for 4 days, and the morphological and ultramicrostructural changes of the cells were observed using inverted microscopy and transmission electron microscopy, respectively. The expression of apoptosis-related protein p27kip1 was detected by immunocytochemistry.


RESULTS
Compared to untreated K562 cells, the cells treated with matrine at 0.10 g/L exhibited apoptostic characteristics in the cellular morphology and ultramicrostructure, with the expression of p27kip1 protein upregulated in a concentration- and time-dependent manner.


CONCLUSIONS
Matrine-induced erythroid differentiation of K562 cells is associated with cell apoptosis, and upregulation of p27kip1 protein expression may play a crucial role in the process of apoptosis.
Zhang
Cui-mei
CM
Department of Child Health Care, Zhongshan Bo'ai Hospital, Southern Medical University, Zhongshan 528403, China. zhangcuimei0527@163.com
Gao
Jian-hui
JH
Li
De-le
DL
Li
Jing
J
Shi
Yu-qi
YQ
Lin
Jun
J
Luo
Shen-qiu
SQ
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007150:Immunohistochemistry; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology; D046529:Microscopy, Electron, Transmission; D011807:Quinolizines / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D011807:Quinolizines; D050760:Cyclin-Dependent Kinase Inhibitor p27; D000093842:Matrines