title: Involvement of purinergic P2 receptors in experimental retinal neovascularization.
abstract: OBJECTIVE
  The present study was aimed to investigate the expression of purinergic P2 receptors in oxygen-induced retinal neovascularization.
  
  
  METHODS
  Immunohistochemistry was used to study the expression of purinergic P2Y2 and P2X2 receptors in the neonatal mouse retina during normal vascular development and after oxygen-induced retinopathy (OIR). The effect of the P2 antagonists, suramin and PPADS, on the extent of oxygen-induced retinal neovascularization was analyzed.
  
  
  RESULTS
  In normal mice, the expression of P2Y2 receptors was weak throughout the retina, whereas P2X2 receptor expression was detected in the outer plexiform layer. In mice treated with oxygen, P2Y2 expression was detected in the ganglion and in the nerve fiber layers, whereas P2X2 expression was found in the inner and outer plexiform layers. Oxygen-induced preretinal neovascularization was strongly inhibited by the P2 antagonists, suramin (p<0.05) and PPADS (p<0.05), and this was accompanied by a down-regulation of P2X2 receptor expression in the inner plexiform layer in suramin-treated mice.
  
  
  CONCLUSIONS
  The data suggest that purinergic P2 receptors are involved in neovascularization associated with OIR.
authors: Department of Clinical Neuroscience, St. Erik's Eye Hospital, Karolinska Institutet, Stockholm, Sweden. sylvia.sarman@sankterik.se
authors: Sylvia
authors: S
authors: Sarman
authors: Jorge
authors: J
authors: Mancini
authors: Ingeborg
authors: I
authors: van der Ploeg
authors: J Oscar
authors: JO
authors: Croxatto
authors: Anders
authors: A
authors: Kvanta
authors: Juan E
authors: JE
authors: Gallo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D019084:Fluorescent Antibody Technique, Indirect; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010100:Oxygen / Q000633:toxicity; D058919:Purinergic P2 Receptor Antagonists; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058476:Receptors, Purinergic P2X2; D058473:Receptors, Purinergic P2Y2; D012160:Retina / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015861:Retinal Neovascularization / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D013498:Suramin / Q000494:pharmacology
chemical_list: C546402:P2rx2 protein, mouse; C546386:P2ry2 protein, mouse; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D058476:Receptors, Purinergic P2X2; D058473:Receptors, Purinergic P2Y2; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D011732:Pyridoxal Phosphate; D013498:Suramin; D010100:Oxygen