Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica.
Complete sequences of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna are presented. In particular, small subunit (18S) and internal transcribed spacers (ITS1 and ITS2) of the ribosomal gene (rDNA), as well as cytochrome c oxidase subunit I (cox1) and nicotinamide dehydrogenase subunit I (nad1) of the mitochondrial DNA (mtDNA), were analyzed. The 18S and ITS sequences were compared with previously published sequences of the liver fluke Fasciola hepatica. Fixed interspecific genetic differences were determined that allow molecular differentiation of F. magna and F. hepatica using either the PCR-RFLP method or PCR amplification of species-specific DNA regions. Additionally, intraspecific sequence polymorphism of the complete cox1 and nad1 mitochondrial genes in geographically distinct F. magna populations was determined. Based on the sequence divergences, short (< 500 bp) variable regions suitable for broader biogeographical studies of giant liver fluke were designed.
Králová-Hromadová
Ivica
I
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Kosice, Slovakia. hromadova@saske.sk
Spakulová
Marta
M
Horácková
Eva
E
Turceková
Ludmila
L
Novobilský
Adam
A
Beck
Relja
R
Koudela
Bretislav
B
Marinculić
Albert
A
Rajský
Dusan
D
Pybus
Margo
M
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D051545:Cyclooxygenase 1 / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D003670:Deer; D005210:Fasciola hepatica / Q000145:classification / Q000235:genetics*; D005212:Fasciolidae / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D008099:Liver / Q000469:parasitology; D008969:Molecular Sequence Data; D009243:NAD / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
D018509:DNA, Helminth; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D009243:NAD; D051545:Cyclooxygenase 1