title: Beta and gamma-sarcoglycans are decreased in the detrusor smooth muscle cells of the partially obstructed rabbit bladder.
abstract: OBJECTIVE
  We evaluated and quantified the levels of sarcoglycans present in the detrusor muscle layer of rabbits with partial bladder outlet obstruction.
  
  
  METHODS
  Rabbits underwent surgery, as previously described, to partially obstruct the urethra. One, 3, 7 and 14 days after obstruction the detrusor muscle layer was dissected free of the remaining bladder tissue and extracted with detergent to isolate the transmembrane components of the dystroglycan-glycoprotein complex. Several components of the dystroglycan-glycoprotein complex were characterized and quantified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.
  
  
  RESULTS
  Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis several bands were noted on gels with a molecular weight (43 and 35 kDa, respectively) corresponding to beta and gamma-sarcoglycan. As obstruction progressed longitudinally, the levels of beta and gamma-sarcoglycan showed progressive decrease at the protein level with beta-sarcoglycan levels recovering at later time points. Bladders with a functional physiology that showed more advanced symptoms of dysfunction had a greater decrease in beta and gamma-sarcoglycan protein.
  
  
  CONCLUSIONS
  The levels of beta and gamma-sarcoglycan progressively change with obstruction with greater changes occurring in the levels of gamma-sarcoglycan. It is likely that alterations in the dystroglycan-glycoprotein complex are responsible for some of the changes in muscle physiology that occur as a consequence of obstruction.
authors: Department of Anatomy and Cell Biology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors: Wenjie
authors: W
authors: Wei
authors: Pamela S
authors: PS
authors: Howard
authors: Steven A
authors: SA
authors: Zderic
authors: Edward J
authors: EJ
authors: Macarak
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism; D011817:Rabbits; D049031:Sarcoglycans / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism*
chemical_list: C483098:SGCB protein, human; C526573:SGCG protein, human; D049031:Sarcoglycans