DNA-binding properties studies and spectra of a novel Cu(II) complex with a new coumarin derivative.
A new coumarin derivative (8-methylcoumaro-4a,10a-pyrone-3-carbaldehyde benzoyl hydrazone) ligand and its novel Cu(II) complex have been synthesized and characterized on the basis of elemental analyses, molar conductivities, (1)H-NMR, IR spectra, UV-visible spectroscopy and thermal analyses. In addition, the interactions of the Cu(II) complex and the ligand with calf-thymus DNA were investigated by spectrometric titrations, ethidium bromide displacement experiments and viscosity measurements. It was found that both the two compounds, specially the Cu(II) complex, strongly bind with calf-thymus DNA, presumably via an intercalation mechanism.
Qi
Gao-Fei
GF
College of Chemistry and Chemical Engineering and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou University, PR China.
Yang
Zheng-Yin
ZY
Qin
Dong-Dong
DD
Wang
Bao-Dui
BD
Li
Tian-Rong
TR
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003300:Copper / Q000737:chemistry*; D003374:Coumarins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D007364:Intercalating Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity
D003374:Coumarins; D007364:Intercalating Agents; D008024:Ligands; C529357:methylcoumaro-4a,10a-pyrone-3-carbaldehyde; D003300:Copper; D004247:DNA; D004996:Ethidium