title: Promiscuous peptides on the nontypeable Haemophilus influenzae P6 outer membrane protein.
abstract: BACKGROUND
  P6 outer membrane protein is one of the candidates for a vaccine formulation against nontypeable Haemophilus influenzae (NTHi) infection. As otitis-prone children who have recurrent episodes of acute otitis media because of NTHi show an impaired immune response to P6, an innovative approach to vaccination is required to augment their immune response.
  
  
  CONCLUSIONS
  We previously identified human HLA-DR9-restricted T cell epitope peptide and highly immunogenic analog peptides on P6 for peptide vaccine candidates. To develop a vaccine formulation effective in the general population, we identified promiscuous T cell epitope peptides (p41-55, p71-85) on P6. In addition to stimulating with potentially promiscuous peptides (p30-44, p45-59) selected using a computer algorithm, we established peptide-specific T cell lines which respond to P6.
  
  
  CONCLUSIONS
  Our present results indicate that these peptides would be candidates for a widely applicable peptide vaccine formulation.
authors: Department of Otolaryngology-Head and Neck Surgery, Asahikawa Medical College, Midorigaoka E2-1-1-1, Asahikawa, Hokkaido 078-8510, Japan.
authors: Yuka
authors: Y
authors: Nomura
authors: Yusuke
authors: Y
authors: Abe
authors: Yoshiya
authors: Y
authors: Ishida
authors: Hiroya
authors: H
authors: Kobayashi
authors: Yasuaki
authors: Y
authors: Harabuchi
mesh_terms: D000465:Algorithms; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000276:immunology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D018073:Haemophilus Vaccines; D000949:Histocompatibility Antigens Class II; C069990:P6 outer membrane protein, Haemophilus; D010455:Peptides