title: Micropermeability of current self-etching and etch-and-rinse adhesives bonded to deep dentine: a comparison study using a double-staining/confocal microscopy technique.
abstract: Water sorption decreases the mechanical properties and the bond strengths of resin-bonded dentine. The aim of this study was to evaluate the micropermeability of several self-etching and etch-and-rinse adhesives. Optibond FL, Silorane, Scotchbond 1XT, G-Bond, and DC-Bond were bonded under simulated pulpal pressure. A 10 wt% solution of ammoniacal silver nitrate and a 1 wt% solution of rhodamine B were injected into the pulp chamber at 20 cm of water pressure. The dentine-adhesive interfaces were examined using a confocal scanning microscope. Micropermeability was detected in all the adhesives. DC-Bond, G-Bond, and Scotchbond 1XT showed voids along the resin-bonded interface. Silorane and Optibond FL showed an adhesive layer that was free from water trees and micropermeability. The double staining technique is a method that gives accurate results in the study of the resin-dentine micropermeability. Each class of adhesive has a different distribution of micropermeability. The higher the micropermeability, the higher the risk of defects at the resin-dentine interface, which may represent the pathway for hydrolytic and enzymatic degradation of resin-dentine bonds over time.
authors: Dental Biomaterials Science, Biomimetics and Biophotonics, King's College London Dental Institute at Guy's, St Thomas Hospitals, London, UK. salvatore.sauro@kcl.ac.uk
authors: Salvatore
authors: S
authors: Sauro
authors: David H
authors: DH
authors: Pashley
authors: Francesco
authors: F
authors: Mannocci
authors: Franklin R
authors: FR
authors: Tay
authors: Peter
authors: P
authors: Pilecki
authors: Martyn
authors: M
authors: Sherriff
authors: Timothy F
authors: TF
authors: Watson
mesh_terms: D000328:Adult; D001840:Dental Bonding; D045662:Dental Etching / Q000379:methods; D003782:Dental Pulp; D003804:Dentin; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D006801:Humans; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D018613:Microscopy, Confocal; D019279:Resin Cements; D012235:Rhodamines; D016622:Silver Staining
chemical_list: D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; D019279:Resin Cements; D012235:Rhodamines; C029773:rhodamine B