Mesoporous silica nanoparticles inhibit cellular respiration.
We studied the effect of two types of mesoporous silica nanoparticles, MCM-41 and SBA-15, on mitochondrial O 2 consumption (respiration) in HL-60 (myeloid) cells, Jurkat (lymphoid) cells, and isolated mitochondria. SBA-15 inhibited cellular respiration at 25-500 microg/mL; the inhibition was concentration-dependent and time-dependent. The cellular ATP profile paralleled that of respiration. MCM-41 had no noticeable effect on respiration rate. In cells depleted of metabolic fuels, 50 microg/mL SBA-15 delayed the onset of glucose-supported respiration by 12 min and 200 microg/mL SBA-15 by 34 min; MCM-41 also delayed the onset of glucose-supported respiration. Neither SBA-15 nor MCM-41 affected cellular glutathione. Both nanoparticles inhibited respiration of isolated mitochondria and submitochondrial particles.
Tao
Zhimin
Z
Department of Pediatrics, State University of New York, Upstate Medical University, 750 East Adams Street, Syracuse, New York 13210, USA.
Morrow
Matthew P
MP
Asefa
Tewodros
T
Sharma
Krishna K
KK
Duncan
Cole
C
Anan
Abhishek
A
Penefsky
Harvey S
HS
Goodisman
Jerry
J
Souid
Abdul-Kader
AK
D002460:Cell Line; D019069:Cell Respiration / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D053758:Nanoparticles / Q000008:administration & dosage* / Q000648:ultrastructure*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000494:pharmacology*
D012822:Silicon Dioxide