Solvent-free matrix dry-coating for MALDI imaging of phospholipids.
A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-microm stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods.
Puolitaival
Satu M
SM
Department of Chemistry, Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee, USA.
Burnum
Kristin E
KE
Cornett
D Shannon
DS
Caprioli
Richard M
RM
D008279:Magnetic Resonance Imaging / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D011208:Powders; D012997:Solvents / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D010743:Phospholipids; D011208:Powders; D012997:Solvents