Transplants of encapsulated rat choroid plexus cells exert neuroprotection in a rodent model of Huntington's disease.
Choroid plexus (CP) epithelial cells secrete several neurotrophic factors and have been used in transplantation studies designed to impart neuroprotection against central nervous system (CNS) trauma. In the present study, CP was isolated from adult rats, encapsulated within alginate microcapsules, and transplanted unilaterally into the rat striatum. Three days later, unilateral injections of quinolinic acid (QA; 225 nmol) were made into the ipsilateral striatum to mimic the pathology observed in Huntington's disease (HD). After surgery, animals were tested for motor function using the placement test. Rats receiving CP transplants were significantly less impaired on this test. Nissl-stained sections demonstrated that CP transplants significantly reduced the volume of the striatal lesion produced by QA. Quantitative analysis of striatal neurons further demonstrated that choline acetyltransferase-immunoreactive, but not diaphorase-positive, neurons were protected by CP transplants. These data demonstrate that transplanted CP cells can be used to protect striatal neurons from excitotoxic damage and that the pattern of neuroprotection varies across specific neuronal populations.
Borlongan
Cesario V
CV
Neurology/Institute of Molecular Medicine and Genetics/School of Graduate Studies, Medical College of Georgia, Augusta, GA 30912-3200, USA. cborlongan@mail.mcg.edu
Thanos
Christopher G
CG
Skinner
Steven J M
SJ
Geaney
Marilyn
M
Emerich
Dwaine F
DF
D000464:Alginates; D000818:Animals; D002214:Capsules; D002831:Choroid Plexus / Q000166:cytology*; D003342:Corpus Striatum / Q000473:pathology; D004847:Epithelial Cells / Q000637:transplantation*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006816:Huntington Disease / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D008297:Male; D009043:Motor Activity; D009410:Nerve Degeneration / Q000139:chemically induced / Q000628:therapy; D009474:Neurons / Q000473:pathology; D017378:Quinolinic Acid; D051381:Rats; D017208:Rats, Wistar
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D017378:Quinolinic Acid