Autophagic machinery activated by dengue virus enhances virus replication.
Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication.
Lee
Ying-Ray
YR
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Lei
Huan-Yao
HY
Liu
Ming-Tao
MT
Wang
Jen-Ren
JR
Chen
Shun-Hua
SH
Jiang-Shieh
Ya-Fen
YF
Lin
Yee-Shin
YS
Yeh
Trai-Ming
TM
Liu
Ching-Chuan
CC
Liu
Hsiao-Sheng
HS
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D000071187:Autophagy-Related Protein 5; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006224:Cricetinae; D003716:Dengue Virus / Q000472:pathogenicity* / Q000502:physiology; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010588:Phagosomes / Q000648:ultrastructure; D014779:Virus Replication / Q000502:physiology*; D052118:Lysosomal-Associated Membrane Protein 1
D000071187:Autophagy-Related Protein 5; D051907:Lysosomal Membrane Proteins; D008869:Microtubule-Associated Proteins; C502778:ATG5 protein, human; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; C477012:MAP1LC3A protein, human