title: Ryanodine receptors decant internal Ca2+ store in human and bovine airway smooth muscle.
abstract: Several putative roles for ryanodine receptors (RyR) were investigated in human and bovine airway smooth muscle. Changes in intracellular Ca2+ concentration ([Ca2+]i) and membrane current were investigated in single cells by confocal fluorimetry and patch-clamp electrophysiology, respectively, whereas mechanical activity was monitored in intact strips with force transducers. RyR released Ca2+ from the sarcoplasmic reticulum in a ryanodine- and chloroethyl phenol (CEP)-sensitive fashion. Neither ryanodine nor CEP inhibited responses to KCl, cholinergic agonists or serotonin, indicating no direct role for RyR in contraction; in fact, there was some augmentation of these responses. In tissues pre-contracted with carbachol, the concentration-response relationships for isoproterenol and salmeterol were unaffected by ryanodine; relaxations due to a nitric oxide donor were also largely unaffected. Finally, it was examined whether RyR were involved in regulating [Ca2+]i within the subplasmalemmal space using patch-clamp electrophysiology as well as Ca2+ fluorimetry: isoproterenol increased [Ca2+]i- and Ca2+-dependent K+ current activity in a ryanodine-sensitive fashion. In conclusion, ryanodine receptors in airway smooth muscle are not important in directly mediating contraction or relaxation. The current authors speculate instead that these allow the sarcoplasmic reticulum to release Ca2+ towards the plasmalemma (to unload an overly full Ca2+ store and/or increase the Ca2+-buffering capacity of the sarcoplasmic reticulum) without affecting bronchomotor tone.
authors: Firestone Institute for Respiratory Health, St Joseph's Hospital, Hamilton, ON, Canada.
authors: T
authors: T
authors: Tazzeo
authors: Y
authors: Y
authors: Zhang
authors: S
authors: S
authors: Keshavjee
authors: L J
authors: LJ
authors: Janssen
mesh_terms: D000818:Animals; D001980:Bronchi / Q000473:pathology; D016084:Bronchoconstriction; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D004594:Electrophysiology / Q000379:methods; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D018482:Muscle, Skeletal / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D019800:Phenol / Q000494:pharmacology; D011188:Potassium / Q000737:chemistry; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*
chemical_list: D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D019800:Phenol; D011188:Potassium; D002118:Calcium