Recognition of tRNALeu by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps.
Recognition of tRNA by the cognate aminoacyl-tRNA synthetase during translation is crucial to ensure the correct expression of the genetic code. To understand tRNA(Leu) recognition sets and their evolution, the recognition of tRNA(Leu) by the leucyl-tRNA synthetase (LeuRS) from the primitive hyperthermophilic bacterium Aquifex aeolicus was studied by RNA probing and mutagenesis. The results show that the base A73; the core structure of tRNA formed by the tertiary interactions U8-A14, G18-U55 and G19-C56; and the orientation of the variable arm are critical elements for tRNA(Leu) aminoacylation. Although dispensable for aminoacylation, the anticodon arm carries discrete editing determinants that are required for stabilizing the conformation of the post-transfer editing state and for promoting translocation of the tRNA acceptor arm from the synthetic to the editing site.
Yao
Peng
P
State Key Laboratory of Molecular Biology - Graduate School of the Chinese Academy of Sciences, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Sciences, Shanghai, People's Republic of China.
Zhu
Bin
B
Jaeger
Sophie
S
Eriani
Gilbert
G
Wang
En-Duo
ED
D000596:Amino Acids / Q000737:chemistry; D000926:Anticodon / Q000737:chemistry; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D007455:Iodine; D007935:Leucine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D023302:Protein Footprinting; D012356:RNA, Transfer, Leu / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012260:Ribonucleases; D013379:Substrate Specificity; D046249:Transfer RNA Aminoacylation
D000596:Amino Acids; D000926:Anticodon; D012356:RNA, Transfer, Leu; D007455:Iodine; D012260:Ribonucleases; D007935:Leucine-tRNA Ligase