title: Cellular basis for arrhythmogenesis in an experimental model of the SQT1 form of the short QT syndrome.
abstract: BACKGROUND
  Short QT syndrome (SQTS) is a primary electrical disease of the heart associated with a high risk of sudden cardiac death. A gain-of-function in I(Kr), due to a mutation in KCNH2, underlies SQT1.
  
  
  OBJECTIVE
  This study sought to examine the cellular basis for arrhythmogenesis in an experimental model of SQT1 created using PD-118057, a novel I(Kr) agonist.
  
  
  METHODS
  Transmembrane action potentials were simultaneously recorded from epicardial, M, and endocardial regions of arterially perfused canine left ventricular (LV) wedge preparations, together with a pseudo-electrocardiogram.
  
  
  RESULTS
  PD-118057 (10 micromol/l) abbreviated the QT interval from 267 +/- 4 to 232 +/- 4 ms and increased transmural dispersion of repolarization (TDR) from 33.7 +/- 2.0 to 49.1 +/- 3.1 ms (P <.001). T-wave amplitude increased from 18.0% +/- 1.4% to 23.1% +/- 1.7% of R-wave amplitude (P =.027). Reversing the direction of activation of the LV wall (epicardial pacing) resulted in an increase in QT interval from 269 +/- 5 to 282 +/- 5 ms and an increase in TDR from 34.1 +/- 2.0 to 57.6 +/- 3.3 ms (P <.001) under baseline conditions. PD-118057 abbreviated the QT interval from 282 +/- 5 to 258 +/- 5 ms and produced a proportional decrease in effective refractory period (ERP). TDR increased from 57.6 +/- 3.3 to 77.6 +/- 4.3 ms (P <.001). Polymorphic ventricular tachycardia (pVT) was induced in 10 of 20 preparations with a single S(2) applied to epicardium. Quinidine (10 micromol/l) increased the ERP and QT interval, did not significantly alter TDR, and prevented induction of pVT in 5 of 5 preparations.
  
  
  CONCLUSIONS
  Our results suggest that a combination of ERP abbreviation and TDR amplification underlie the development of pVT in SQT1 and that quinidine prevents pVT principally by prolonging ERP.
authors: Masonic Medical Research Laboratory, Utica, New York 13501, USA.
authors: Chinmay
authors: C
authors: Patel
authors: Charles
authors: C
authors: Antzelevitch
mesh_terms: D000200:Action Potentials / Q000235:genetics; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D001145:Arrhythmias, Cardiac / Q000235:genetics / Q000503:physiopathology*; D002722:Chlorobenzenes; D016757:Death, Sudden, Cardiac / Q000209:etiology* / Q000473:pathology*; D004285:Dogs; D000072237:ERG1 Potassium Channel; D004594:Electrophysiology; D004699:Endocardium / Q000503:physiopathology; D051638:Ether-A-Go-Go Potassium Channels / Q000235:genetics; D006352:Heart Ventricles / Q000503:physiopathology; D008564:Membrane Potentials; D023421:Models, Animal; D008962:Models, Theoretical; D010496:Pericardium / Q000503:physiopathology; D015221:Potassium Channels / Q000187:drug effects; D011802:Quinidine / Q000494:pharmacology; D012307:Risk Factors; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
chemical_list: C517140:2-(4-(2-(3,4-dichlorophenyl)ethyl)phenylamino)benzoic acid; D000889:Anti-Arrhythmia Agents; D002722:Chlorobenzenes; D000072237:ERG1 Potassium Channel; D051638:Ether-A-Go-Go Potassium Channels; D015221:Potassium Channels; D062367:ortho-Aminobenzoates; D011802:Quinidine