title: Dynamic remodeling of individual nucleosomes across a eukaryotic genome in response to transcriptional perturbation.
abstract: The eukaryotic genome is packaged as chromatin with nucleosomes comprising its basic structural unit, but the detailed structure of chromatin and its dynamic remodeling in terms of individual nucleosome positions has not been completely defined experimentally for any genome. We used ultra-high-throughput sequencing to map the remodeling of individual nucleosomes throughout the yeast genome before and after a physiological perturbation that causes genome-wide transcriptional changes. Nearly 80% of the genome is covered by positioned nucleosomes occurring in a limited number of stereotypical patterns in relation to transcribed regions and transcription factor binding sites. Chromatin remodeling in response to physiological perturbation was typically associated with the eviction, appearance, or repositioning of one or two nucleosomes in the promoter, rather than broader region-wide changes. Dynamic nucleosome remodeling tends to increase the accessibility of binding sites for transcription factors that mediate transcriptional changes. However, specific nucleosomal rearrangements were also evident at promoters even when there was no apparent transcriptional change, indicating that there is no simple, globally applicable relationship between chromatin remodeling and transcriptional activity. Our study provides a detailed, high-resolution, dynamic map of single-nucleosome remodeling across the yeast genome and its relation to global transcriptional changes.
authors: Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, and Section of Molecular Genetics and Microbiology, University of Texas at Austin,Austin, Texas, United States of America.
authors: Sushma
authors: S
authors: Shivaswamy
authors: Akshay
authors: A
authors: Bhinge
authors: Yongjun
authors: Y
authors: Zhao
authors: Steven
authors: S
authors: Jones
authors: Martin
authors: M
authors: Hirst
authors: Vishwanath R
authors: VR
authors: Iyer
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D042002:Chromatin Assembly and Disassembly; D005057:Eukaryotic Cells / Q000378:metabolism*; D016678:Genome / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000235:genetics* / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016385:TATA Box; D014157:Transcription Factors / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D009707:Nucleosomes; D014157:Transcription Factors