Supramolecular interaction of ethylenediamine linked beta-cyclodextrin dimer and berberine hydrochloride by spectrofluorimetry and its analytical application.
The supramolecular interaction of beta-cyclodextrin dimer with berberine hydrochloride was studied in aqueous KH2PO4-H3PO4 buffer solution of pH 2.00 at room temperature by spectrofluorimetry. The apparent association constant of the complex was 1.53 x 10(4) L mol(-1). Based on the significant enhancement of fluorescence intensity of supramolecular sandwich complexes, a spectrofluorimetric method with high sensitivity and selectivity was developed for the determination of berberine hydrochloride in aqueous solution in presence of ethylenediamine linked beta-CD dimer. The linear range of the method was 12.8-1.00 x 10(4 )ng mL(-1) with the detection limit 3.6 ng mL(-1). There was no interference from the normally used in tablets and serum constituents. The proposed method was successfully applied to the determination of berberine hydrochloride in tablets and serum. And then it has a promising potential in therapeutic drug monitoring, pharmacokinetics and clinical application.
Liu
Fang
F
College of Chemistry, Chemical Engineering and Materials Science, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Shandong Normal University, Jinan 250014, PR China.
Liang
Hui-Ling
HL
Xu
Ke-Hua
KH
Tong
Li-Li
LL
Tang
Bo
B
D000328:Adult; D001599:Berberine / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D005029:Ethylenediamines / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D013050:Spectrometry, Fluorescence; D013607:Tablets; D013696:Temperature; D047392:beta-Cyclodextrins / Q000737:chemistry*
D005029:Ethylenediamines; D013607:Tablets; D047392:beta-Cyclodextrins; D001599:Berberine; C031234:ethylenediamine; C031215:betadex