title: Two possible cases of Trichosporon infections in bone-marrow-transplanted children: the first case of T. japonicum isolated from clinical specimens.
abstract: Trichosporon spp. are emerging as opportunistic agents that cause systemic diseases in immunocompromised hosts. Trichosporonosis carries a poor prognosis in neutropenic patients. Trichosporon japonicum was isolated from the air and named by Sugita et al. Here we present the first case of T. japonicum isolated from a clinical specimen. Two cases of acute myeloid leukemia who had Trichosporon isolates are discussed because of their rarity and growing importance. T. asahii was isolated from the throat, feces and urine of the first patient. T. japonicum was isolated from the sputum of the second patient. Both cases produced high MICs to itraconazole, and low MICs to fluconazole and voriconazole. In virulance factor investigations there was (++) biofilm formation in T. japonicum but not in T. asahii. Conventional mycological studies were not adequate for the identification of the isolate at the species level. In our second case as in the first one, the isolate was identified as T. asahii with 99.9% accuracy by API 20C AUX. Although two T. asahii isolates from the same patient yielded identical typing profiles by arbitrary primed-PCR, the isolates of the two different patients showed different arbitrary primed-PCR typing profiles. However, the genetic identification of the other patient's strain gave the result of T. japonicum.
authors: Our-Children Leukemia Foundation, Istanbul, Turkey. agirbaslihandan@hotmail.com
authors: Handan
authors: H
authors: Ağirbasli
authors: Hulya
authors: H
authors: Bilgen
authors: Sema Keceli
authors: SK
authors: Ozcan
authors: Baris
authors: B
authors: Otlu
authors: Gulce
authors: G
authors: Sinik
authors: Nilgün
authors: N
authors: Cerikçioğlu
authors: Riza
authors: R
authors: Durmaz
authors: Emine
authors: E
authors: Can
authors: Nevin
authors: N
authors: Yalman
authors: Gunduz
authors: G
authors: Gedikoğlu
authors: Takashi
authors: T
authors: Sugita
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use; D000935:Antifungal Agents / Q000627:therapeutic use; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002648:Child; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D015255:Idarubicin / Q000627:therapeutic use; D016867:Immunocompromised Host; D007223:Infant; D017964:Itraconazole / Q000627:therapeutic use; D015470:Leukemia, Myeloid, Acute / Q000628:therapy*; D008297:Male; D009181:Mycoses / Q000150:complications* / Q000188:drug therapy; D009503:Neutropenia / Q000150:complications; D009894:Opportunistic Infections / Q000150:complications / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014250:Trichosporon / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D017964:Itraconazole; D015255:Idarubicin