Stretch-activated currents in cardiomyocytes isolated from rabbit pulmonary veins.
Evidence is growing of a relationship between atrial dilation and atrial fibrillation (AF), the most prevalent type of arrhythmia. Pulmonary veins, which are important ectopic foci for provoking AF, are of increasing interest in relation to the early development of AF. Here, using single cardiomyocytes isolated from rabbit pulmonary veins, we characterised the stretch-activated currents induced by swelling and axial mechanical stretching. Swelling induced both a stretch-activated nonselective cationic current (NSC) and a Cl(-) current. The swelling-induced Cl(-) current (I Cl,swell) was inhibited by DIDS, whereas the swelling-induced NSC (I NSC,swell) was inhibited by Gd3+. The cationic selectivity of the I NSC,swell was K+ >Cs+ >Na+ >Li+, whilst the PK/PNa, PCs/PNa, and PLi/PNa permeability ratios were 2.84, 1.86, and 0.85, respectively. Activation of the I NSC,swell was faster than that of the I Cl,swell. Given a high K+ concentration in the bath solution, the I NSC,swell showed limited amplitude (<-70 mV). Mechanical stretching induced an immediate Gd3+- and streptomycin-sensitive NSC (I NSC,stretch) that was permeable to Na+, K+, Cs+ and NMDG. Persistent stretching activated a DIDS-sensitive current (I Cl,stretch). The I NSC,stretch, but not the I NSC,swell, was completely blocked by 400 microM streptomycin; therefore, the two currents may not be associated with the same channel. In addition, the type of current induced may depend on the type of stretching. Thus, stretch-induced anionic and cationic currents are functionally present in the cardiomyocytes of the main pulmonary veins of rabbits, and they may have pathophysiological roles in the development of AF under stretched conditions.
Seol
Chang Ahn
CA
Department of Physiology, University of Ulsan College of Medicine, 388-1 Poongnap-Dong Songpa-Ku, Seoul, Republic of Korea.
Kim
Won Tae
WT
Ha
Jeong Mi
JM
Choe
Han
H
Jang
Yeon Jin
YJ
Youm
Jae Boum
JB
Earm
Yung E
YE
Leem
Chae Hun
CH
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001281:Atrial Fibrillation / Q000503:physiopathology*; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D017136:Ion Transport; D007477:Ions / Q000378:metabolism; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008564:Membrane Potentials; D032383:Myocytes, Cardiac / Q000502:physiology*; D011667:Pulmonary Veins / Q000166:cytology*; D011817:Rabbits; D013307:Streptomycin / Q000494:pharmacology; D013314:Stress, Mechanical
D007477:Ions; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D013307:Streptomycin