Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays.
A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection.
Fernández-Argüelles
M T
MT
Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería, 8, 33006 Oviedo, Spain.
Costa-Fernández
J M
JM
Pereiro
R
R
Sanz-Medel
A
A
D016604:Aflatoxin B1 / Q000032:analysis*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D053758:Nanoparticles; D011108:Polymers; D045663:Quantum Dots; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000906:Antibodies; D011108:Polymers; D016604:Aflatoxin B1