title: Liposome modified with Mn-porphyrin complex can simultaneously induce antioxidative enzyme-like activity of both superoxide dismutase and peroxidase.
abstract: An antioxidative liposome catalysis that mimics both superoxide dismutase (SOD) and peroxidase (POD) activities has been developed by using the liposomes modified with lipophilic Mn-(5,10,15,20-tetrakis[1-hexadecylpyridium-4-yl]-21H,23H-porphyrin) (Mn-HPyP). The SOD- and POD-like activities of the Mn-HPyP-modified liposome were first investigated by varying the type of phospholipid, such as 1,2-distearyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC). Higher SOD-like activity was obtained in the case of DLPC and DMPC liposomes, in which the ligands were well-dispersed on the membrane in the liquid crystalline phase. The POD-like activity was maximal in the case of DMPC liposome, in which the Mn-HPyP complex was appropriately clustered on the membrane in the gel phase. On the basis of the above results, the co-induction of the SOD and POD activities to eliminate the superoxide and also hydrogen peroxide as a one-pot reaction was finally performed by using the Mn-HPyP-modified DMPC liposome, resulting in an increase in the efficiency of the elimination of both superoxide and hydrogen peroxide.
authors: Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Hiroshi
authors: H
authors: Umakoshi
authors: Kengo
authors: K
authors: Morimoto
authors: Yuji
authors: Y
authors: Ohama
authors: Hideto
authors: H
authors: Nagami
authors: Toshinori
authors: T
authors: Shimanouchi
authors: Ryoichi
authors: R
authors: Kuboi
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism*; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D008081:Liposomes / Q000737:chemistry; D008345:Manganese / Q000737:chemistry*; D015394:Molecular Structure; D010544:Peroxidases / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D008081:Liposomes; D011166:Porphyrins; D008345:Manganese; D010544:Peroxidases; D013482:Superoxide Dismutase