Binding of tau protein to the ends of ex vivo paired helical filaments.
Human recombinant tau can bind to the end of isolated human paired helical filaments (PHF). The sequential binding of tau protein to PHF could result in an elongation of the previously polymerized PHF. However, we have observed that the elongation takes place in a different way on different types of PHF. We have found that there are at least three populations of PHF. For one population, tau protein is able to bind to the ends of the filament and to elongate that filament. In the second PHF population, tau protein binds but does not elongates the filament. In the third, neither tau binding nor elongation was observed.
Santa-Maria
Ismael
I
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Cuadros
Raquel
R
Moreno
Francisco J
FJ
Muñoz
Victor
V
Avila
Jesús
J
Hernández
Félix
F
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002232:Carbocyanines; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism; D008968:Molecular Conformation; D050257:Tubulin Modulators / Q000378:metabolism; D016875:tau Proteins / Q000235:genetics* / Q000378:metabolism*
D002232:Carbocyanines; D005456:Fluorescent Dyes; D007381:Intermediate Filament Proteins; D050257:Tubulin Modulators; C089187:cyanine dye 3; D016875:tau Proteins