Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes.
A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.
Gyarmati
Péter
P
Joint Research and Development Division, Department of Virology, National Veterinary Institute and Swedish University of Agricultural Sciences, Ulls väg 2B, SE-751 89 Uppsala, Sweden. peter.gyarmati@sva.se
Conze
Tim
T
Zohari
Siamak
S
LeBlanc
Neil
N
Nilsson
Mats
M
Landegren
Ulf
U
Banér
Johan
J
Belák
Sándor
S
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology*; D009439:Neuraminidase / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D009975:Orthomyxoviridae / Q000145:classification* / Q000235:genetics*; D011200:Poultry; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics
D017931:DNA Primers; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014764:Viral Proteins; C109963:hemagglutinin, avian influenza A virus; D009439:Neuraminidase