title: New chromogenic substrates for feruloyl esterases.
abstract: Indolyl and nitrophenyl 5-O-hydroxycinnamoyl-alpha-L-arabinofuranosides were prepared by chemo-enzymatic syntheses. These probes were designed as substrates to be used in assays of feruloyl esterase activity (EC 3.1.1.77). Color development in the assays only occurs when feruloyl esterase activity releases an intermediate chromogenic arabinoside that is a suitable substrate for alpha-L-arabinofuranosidase (EC 3.2.1.55), which in turn releases the free chromogenic group. The usefulness of these compounds was evaluated in both qualitative solid media-based assays and quantitative liquid assays that can be performed in microtiter plates using feruloyl esterases and arabinofuranosidases from various origins.
authors: Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), BP53, 38041, Grenoble cedex 9, France.
authors: Laurence
authors: L
authors: Marmuse
authors: Michèle
authors: M
authors: Asther
authors: Emeline
authors: E
authors: Fabre
authors: David
authors: D
authors: Navarro
authors: Laurence
authors: L
authors: Lesage-Meessen
authors: Marcel
authors: M
authors: Asther
authors: Michael
authors: M
authors: O'Donohue
authors: Sébastien
authors: S
authors: Fort
authors: Hugues
authors: H
authors: Driguez
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000737:chemistry*; D002863:Chromogenic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry; D015394:Molecular Structure; D009596:Nitrophenols / Q000737:chemistry
chemical_list: D002863:Chromogenic Compounds; D015335:Molecular Probes; D009596:Nitrophenols; C071472:2-chloro-4-nitrophenyl acetate; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase