Sub-micromolar affinity of Escherichia coli NikR for Ni(II).
The dissociation constant of Ni(II) for Escherichia coli NikR was determined using three independent techniques, including binding kinetics, and shown to be in the sub-micromolar range.
Diederix
Rutger E M
RE
CNRS, UMR 5249, 17 avenue des martyrs, Grenoble F-38054 cedex 9, France.
Fauquant
Caroline
C
Rodrigue
Agnès
A
Mandrand-Berthelot
Marie-Andrée
MA
Michaud-Soret
Isabelle
I
D029968:Escherichia coli Proteins / Q000378:metabolism*; D009532:Nickel / Q000378:metabolism*; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D029968:Escherichia coli Proteins; C402409:NikR protein, E coli; D012097:Repressor Proteins; D009532:Nickel