title: Altered expression of the chloroplast ATP synthase through site-directed mutagenesis in Chlamydomonas reinhardtii.
abstract: The chloroplast ATP synthase gates the flow of protons out of the thylakoid lumen. In Chlamydomonas reinhardtii deletion of any of the genes for the ATP synthase subunits, or misfolding of the peptides results in photosynthetic membranes devoid of the enzyme (Lemaire and Wollman, J Biol Chem 264:675-685, 1989). This work examines the physiologic response of an algal strain in which the epsilon subunit of the chloroplast ATP synthase has been truncated. Removal of 10 amino acids from the C-terminus of the peptide results in a sharp decrease in the content of the enzyme, but does not result in its exclusion from the thylakoid membranes. The ATP synthase of this mutant strain has a higher rate of ATP hydrolysis than the wild-type enzyme. This strain of C. reinhardtii exhibits reduced growth in the light, dependence on acetate, and a low threshold for the onset of photoinhibition. The role of the ATP synthase in regulating the proton concentration of the lumen is discussed.
authors: Department of Biology, Johns Hopkins University, Baltimore, MD 21218-2685, USA. ejohn@jhu.edu
authors: Eric A
authors: EA
authors: Johnson
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D025222:Chloroplast Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D009154:Mutation; D010788:Photosynthesis; D017386:Sequence Homology, Amino Acid; D020524:Thylakoids / Q000201:enzymology / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D000255:Adenosine Triphosphate; D025222:Chloroplast Proton-Translocating ATPases