[Relationship between the radiosensitivity of hepatic carcinoma cells and their survivin expression levels].
OBJECTIVE
To investigate the relationship between the radiosensitivity of hepatic carcinoma cells and their survivin expression levels.


METHODS
Hepatic carcinoma cell lines HepG2 and SMMC-7721 were irradiated with various doses of 60Co gamma-rays. The cell survival rate, expression of survivin, cell cycle profile and activity of caspase-3 were respectively detected by clonogenic assay, immunocytochemistry, flow cytometry and chromatometry.


RESULTS
The surviving fraction at 2Gy (SF2) of HepG2 and SMMC-7721 were 0.43+/-0.01 vs 0.70+/-0.02, and HepG2 had higher radiosensitivity than SMMC-7721. gamma-rays radiation up-regulated the expression of survivin. SMMC-7721 had a significantly higher expression of survivin than HepG2 (t = 2.81-5.20, P < 0.05). The activity of caspase-3 was more powerful in HepG2 than in SMMC-7721 (t = 6.05-6.72, P < 0.01).


CONCLUSIONS
Survivin may play a critical role in mediating radiation resistance in SMMC-7721 through its up-regulation and caspase-3 dependent manner.
Ma
Xiao-jie
XJ
Department of Oncology, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Chen
Xiao-pin
XP
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000532:radiotherapy; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000528:radiation effects*; D045744:Cell Line, Tumor; D005720:Gamma Rays; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008113:Liver Neoplasms / Q000378:metabolism* / Q000532:radiotherapy; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D000077022:Survivin; D015854:Up-Regulation
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin; D053148:Caspase 3