Complete nucleotide sequence of a maize chlorotic mottle virus isolate from Nebraska.
The complete genome of a maize chlorotic mottle virus isolate from Nebraska (MCMV-NE) was cloned and sequenced. The MCMV-NE genome consists of 4,436 nucleotides and shares 99.5 nucleotide sequence identity with an MCMV isolate from Kansas (MCMV-KS). Of 22 polymorphic sites, most resulted from transition with a clear bias for U to C and C to U substitutions. The MCMV-NE genome was assembled into a single plasmid insert and used as a template to transcribe RNA in vitro. As RNA transcribed from the cloned MCMV-NE genome was infectious to maize plants, sequence differences between MCMV-NE and MCMV-KS are most likely neutral with respect to pathogenicity and virulence.
Stenger
Drake C
DC
USDA-ARS and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583, USA. drake.stenger@ars.usda.gov
French
Roy
R
D003001:Cloning, Molecular; D016679:Genome, Viral; D007615:Kansas; D008969:Molecular Sequence Data; D009329:Nebraska; D010935:Plant Diseases / Q000821:virology; D011110:Polymorphism, Genetic; D013045:Species Specificity; D019183:Tombusviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics; D003313:Zea mays / Q000235:genetics*