The carriage population of Staphylococcus aureus from Mali is composed of a combination of pandemic clones and the divergent Panton-Valentine leukocidin-positive genotype ST152.
Staphylococcus aureus is an important human pathogen, but it appears more commonly in asymptomatic colonization of the nasopharynx than in cases of invasive disease. Evidence concerning the global population structure of S. aureus is limited by the overrepresentation in the multilocus sequence testing database of disease isolates recovered from Western Europe, the Americas, Australia, and Japan. We address this by presenting data from the S. aureus carriage population in Mali, the first detailed characterization of asymptomatic carriage from an African population. These data confirm the pandemic spread of many of the common S. aureus clones in the carriage population. We also note the high frequency (approximately 24%) of a single divergent genotype, sequence type 152 (ST152), which has not previously been recovered from nasal carriage isolates but corresponds to a sporadic Panton-Valentine leukocidin (PVL)-positive, community-acquired methicillin-resistant S. aureus clone noted mostly in Central Europe. We show that 100% of the ST152 isolates recovered from nasal carriage samples in Mali are PVL positive and discuss implications relating to the emergence and spread of this virulent genotype.
Ruimy
Raymond
R
Hôpital Bichat-Claude Bernard, AP-HP, EA 3964, Université Denis Diderot-Paris 7 Medical School, 75870 Paris Cedex, France. raymond.ruimy@bch.ap-hop-paris.fr
Maiga
Aminata
A
Armand-Lefevre
Laurence
L
Maiga
Ibrahim
I
Diallo
Amadou
A
Koumaré
Abdel Karim
AK
Ouattara
Kalilou
K
Soumaré
Sambou
S
Gaillard
Kevin
K
Lucet
Jean-Christophe
JC
Andremont
Antoine
A
Feil
Edward J
EJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D005098:Exotoxins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D007956:Leukocidins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008302:Mali / Q000453:epidemiology; D008875:Middle Aged; D009666:Nose / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin