Production and characteristics of a bioflocculant produced by Bacillus sp. F19.
A bioflocculant-producing bacterium isolated from soil was identified as Bacillus sp. and the bioflocculant produced was named MBFF19. Effects of physico-chemical conditions including pH, carbon sources and nitrogen sources on MBFF19 production were studied. Chemical analyses of the purified bioflocculant MBFF19 indicated that it was a sugar-protein derivative, composed of neutral sugar (3.6%, w/w), uronic acid (37.0%, w/w), amino sugars (0.5%, w/w) and protein (16.4%, w/w). The two neutral sugar components were mannose and glucose and the molar ratio was 1.2:1. Infrared spectrophotometry analysis revealed that MBFF19 contained carboxyl, hydroxyl and methoxyl groups in its structural. Flocculating properties of bioflocculant MBFF19 was examined using kaolin, activated carbon and fly coal suspension. Cation supplement had no positive effects on the flocculating activity whereas the presence of Fe3+ inhibited flocculation. Influences of pH and bioflocculant dosage on the flocculation were also examined.
Zheng
Yan
Y
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Ye
Zhi-Long
ZL
Fang
Xu-Liang
XL
Li
Ya-Hong
YH
Cai
Wei-Min
WM
D001407:Bacillus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005427:Flocculation; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D017550:Spectroscopy, Fourier Transform Infrared
D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D009584:Nitrogen