title: BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken.
abstract: Reflecting physiological changes in oxygen acquisition and regulatory changes in globin transcription, the makeup of globin chains in erythrocytes varies in development and disease. The relationship between the globin chain composition and erythropoietic lineages/niches is not well-understood. Using a combination of proteomic-, genomic-, and intron-based in situ hybridization analyses, we show that the transcripts and protein product of the major adult beta globin, betaA, are present as early as the major embryonic beta globins during chicken primitive erythropoiesis. A rapid rise in betaA percentage is seen from embryonic day (E) 5, reaching adult profile by E7. Our data suggest that betaA locus is active from the onset of primitive erythropoiesis and that beta globin switching during development may reflect a change in relative transcript abundance rather than a strict on/off switch in gene activation.
authors: Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan.
authors: Cantas
authors: C
authors: Alev
authors: Brendan A S
authors: BA
authors: McIntyre
authors: Hiroki
authors: H
authors: Nagai
authors: Masahiro
authors: M
authors: Shin
authors: Kaori
authors: K
authors: Shinmyozu
authors: Lars M
authors: LM
authors: Jakt
authors: Guojun
authors: G
authors: Sheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D004920:Erythropoiesis; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis; D016415:Sequence Alignment
chemical_list: D020543:Proteome; D005914:Globins