title: Protection against influenza A virus by memory CD8 T cells requires reactivation by bone marrow-derived dendritic cells.
abstract: Influenza A virus is the causative agent of an acute inflammatory disease of the airway. Although Abs can prevent infection, disease and death can be prevented by T cell-mediated immunity. Recently, we showed that protection against lethal influenza A (PR8/34) virus infection is mediated by central memory CD8 T cells (T(CM)). In this study, using relB(-/-) mice we began to investigate the role of bone marrow (BM)-derived dendritic cells (DCs) in the mechanism of protection. We found that in the absence of functional DCs, memory CD8 T cells specific for the nucleoprotein epitope (NP(366-374)) fail to protect even after adoptive transfer into naive recipients. Through an analysis of Ag uptake, activation of memory CD8 T cells, and display of peptide/MHC complex by DCs in draining LNs and spleen early after virus infection, we established that lack of protection is associated with defective Ag presentation by BM-derived DCs and defective homing of memory T cells in the lymph nodes draining the airway tract. Collectively, the data suggest that protection against the influenza A virus requires that memory CD8 T cells be reactivated by Ag presented by BM-derived DCs in the lymph nodes draining the site of infection. They also imply that protection depends both on the characteristics of systemic adaptive immunity and on the coordinated interplay between systemic and local immunity.
authors: The Laboratory of Immunology, Department of Medicine and Moores Cancer Center, University of California San Diego, La Jolla CA 92093, USA.
authors: Paola
authors: P
authors: Castiglioni
authors: De Shon
authors: de S
authors: Hall
authors: Erica L
authors: EL
authors: Jacovetty
authors: Elizabeth
authors: E
authors: Ingulli
authors: Maurizio
authors: M
authors: Zanetti
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D007156:Immunologic Memory / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology; D019041:L-Selectin / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009976:Orthomyxoviridae Infections / Q000276:immunology; D012995:Solubility; D051841:Transcription Factor RelB / Q000172:deficiency / Q000235:genetics / Q000378:metabolism
chemical_list: D000941:Antigens; D007252:Influenza Vaccines; C497594:Relb protein, mouse; D019041:L-Selectin; D051841:Transcription Factor RelB