title: Tel/PDGFRbeta inhibits self-renewal and directs myelomonocytic differentiation of ES cells.
abstract: The leukemic oncogene Tel/PDGFRbeta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFRbeta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFRbeta-expressing cells. This study indicates Tel/PDGFRbeta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.
authors: Biomedical Sciences Research Institute, University of Ulster, Cromore Road, Coleraine, Northern Ireland, UK.
authors: E
authors: E
authors: Dobbin
authors: P M
authors: PM
authors: Corrigan
authors: C P
authors: CP
authors: Walsh
authors: M J
authors: MJ
authors: Welham
authors: R W
authors: RW
authors: Freeburn
authors: H
authors: H
authors: Wheadon
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D006410:Hematopoiesis / Q000235:genetics; D053605:Leukemia Inhibitory Factor / Q000494:pharmacology; D051379:Mice; D022423:Myeloid Cells / Q000166:cytology; D038042:Myelopoiesis; D015514:Oncogene Proteins, Fusion / Q000378:metabolism*
chemical_list: D053605:Leukemia Inhibitory Factor; D015514:Oncogene Proteins, Fusion; C120888:TEL-PDGFRbeta fusion protein, human