title: Prnp knockdown in transgenic mice using RNA interference.
abstract: RNA interference has become a widely used approach to perform gene knockdown experiments in cell cultures and more recently transgenic animals. A designed miRNA targeting the prion protein mRNA was built and expressed using the human PRNP promoter. Its efficiency was confirmed in transfected cells and it was used to generate several transgenic mouse lines. Although expressed at low levels, it was found to downregulate the endogenous mouse Prnp gene expression to an extent that appears to be directly related with the transgene expression level and that could reach up to 80% inhibition. This result highlights the potential and limitations of the RNA interference approach when applied to disease resistance.
authors: UR 339, Laboratoire de Génétique Biochimique et de Cytogénétique, Institut National de la Recherche Agronomique, 78350, Jouy-en-Josas, France.
authors: Micaela
authors: M
authors: Gallozzi
authors: Jérome
authors: J
authors: Chapuis
authors: Fabienne
authors: F
authors: Le Provost
authors: Annick
authors: A
authors: Le Dur
authors: Caroline
authors: C
authors: Morgenthaler
authors: Coralie
authors: C
authors: Peyre
authors: Nathalie
authors: N
authors: Daniel-Carlier
authors: Eric
authors: E
authors: Pailhoux
authors: Marthe
authors: M
authors: Vilotte
authors: Bruno
authors: B
authors: Passet
authors: Laetitia
authors: L
authors: Herzog
authors: Vincent
authors: V
authors: Beringue
authors: José
authors: J
authors: Costa
authors: Philippe
authors: P
authors: Tixador
authors: Gaëlle
authors: G
authors: Tilly
authors: Hubert
authors: H
authors: Laude
authors: Jean-Luc
authors: JL
authors: Vilotte
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D015536:Down-Regulation; D005822:Genetic Vectors; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics; D018621:PrPC Proteins / Q000235:genetics; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics*; D034622:RNA Interference
chemical_list: D017931:DNA Primers; D035683:MicroRNAs; C512656:PRNP protein, human; D018621:PrPC Proteins; D000072002:Prion Proteins; D011328:Prions