[Expression of peroxisome proliferator-activated receptor gamma in glioma].
OBJECTIVE
To investigate the expression and significance of peroxisome proliferators-activated receptor gamma (PPAR gamma) in human glioma.


METHODS
Immunohistochemical staining for PPAR gamma was performed using biopsy specimens of human glioma of various histological types. Expression of PPAR gamma and GFAP in glioma cell lines SWO-38, U251 and SHG-44 were analyzed using Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).


RESULTS
Immunohistochemical study showed that PPAR gamma was expressed in glioma tissues with positive rate of 37.5%. Western blotting and RT-PCR showed that PPAR gamma was expressed in both glioma cell lines SWO-38 and U251, but not in SHG-44 cells. However, high expression of GFAP was detected in SHG-44 cells.


CONCLUSIONS
PPAR gamma is associated with carcinogens of glioma. Actived PPAR gamma by agonist may be a novel approach to the treatment of glioma.
Wang
Ming-hua
MH
Department of Pathology, Chongqing Medical Oniversity, Chongqing 410016, China. wangmhhh@yahoo.cn
Zhong
Xue-yun
XY
Lin
Chen-li
CL
Xie
You-ke
YK
Jia
Jin-ping
JP
Li
Su-mei
SM
Mi
Can
C
D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis / Q000235:genetics; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005904:Glial Fibrillary Acidic Protein; D047495:PPAR gamma; D012333:RNA, Messenger