The use of syntaxin chimeras to study polarized protein trafficking in epithelial cells.
The plasma membrane of epithelial cells has two physically separated membrane domains. This membrane polarization is essential for the function of epithelial cells. It has been well established that different plasma membrane syntaxin forms are expressed in epithelial cells. In addition, these syntaxin forms can have a polarized localization, suggesting that they may play a direct role in the specificity of polarized membrane delivery. To determine the mechanism of the polarized syntaxin localization, we have made several chimeras of syntaxin 3 and 4. This allowed us to identify the protein sequences involved in this polarized localization. Using this technique, we showed that targeting information of syntaxin 3 and 4 is located in the first 30 amino acids.
ter Beest
Martin B A
MB
Department of Surgery, The University of Chicago, Chicago, IL, USA.
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D015153:Blotting, Western; D002460:Cell Line; D016764:Cell Polarity; D051152:Clusterin / Q000378:metabolism; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors; D018613:Microscopy, Confocal; D021381:Protein Transport; D050765:Qa-SNARE Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013997:Time Factors; D014161:Transduction, Genetic
D051152:Clusterin; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins