Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts.
The antioxidant potential of silk protein sericin from the non-mulberry tropical tasar silkworm Antheraea mylitta cocoon has been assessed and compared with that of the mulberry silkworm, Bombyx mori. Skin fibroblast cell line (AH927) challenged with hydrogen peroxide served as the positive control for the experiment. Our results showed that the sericin obtained from tasar cocoons offers protection against oxidative stress and cell viability is restored to that of control on pre-incubation with the sericin. Fibroblasts pre-incubated with non-mulberry sericin had significantly lower levels of catalase; lactate dehydrogenase and malondialdehyde activity when compared to untreated ones. This report indicates that the silk protein sericin from the non-mulberry tropical tasar silkworm, A. mylitta can serve as a valuable antioxidant.
Dash
Rupesh
R
Department of Biotechnology, Indian Institute of Technology, Kharagpur, India-721 302.
Acharya
Chitrangada
C
Bindu
P C
PC
Kundu
S C
SC
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D012831:Bombyx; D002374:Catalase / Q000378:metabolism; D002415:Cats; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects* / Q000201:enzymology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D047030:Sericins / Q000032:analysis / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*
D000975:Antioxidants; D047030:Sericins; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase