Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis.
OBJECTIVE
To study the effect of the modulation of inositol hexaphosphate (IP6) in the biological immunohistochemistry expression of cellular signaling marker apoptosis, in model of carcinogenesis of colon induced by azoxymethane (AOM).


METHODS
Wistar rats (N=112) distributed in 4 groups (n=28): Control; B, AOM (5 mg kg-1, 2x, to break week 3); C, IP6 (in water 1%, six weeks); D, IP6+AOM. Weekly euthanasia (n=7), from week three. Immunohistochemistry of ascendant colon with biological marker inositol 1,4,5 triphosphate receptor type III (Itpr3). Quantification of the immune-expression with use of computer-assisted image processing. Analysis statistics of the means between groups, weeks in groups, groups in weeks, and established significance when p<or=0.05.


RESULTS
One proved significant difference between groups in the expression of Itpr3, p<0.0001; with Itpr3 reduction of BxD group, p<0.001.


CONCLUSIONS
Inositol hexaphosphate promotes modulation of biological markers with reduction of Itpr3 in carcinogenesis of colon.
Guido
Marks
M
Program in Surgery and Experimentation, Department of Surgery, UNIFESP-SP, São Paulo, Brazil. gmarks@brturbo.com.br
Fagundes
Djalma José
DJ
Ynouye
Celso Massaschi
CM
Pontes
Elenir Rose Jardim Cury
ER
Takita
Luiz Carlos
LC
Siufi do Amaral
Eva Glória
EG
Teruya
Roberto
R
Paes
Manoel Catarino
MC
Brasileiro
José Lacerda
JL
Aydos
Ricardo Dutra
RD
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001397:Azoxymethane; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002273:Carcinogens; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism*; D007150:Immunohistochemistry; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism*; D008297:Male; D010833:Phytic Acid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D014408:Biomarkers, Tumor; D002273:Carcinogens; D053496:Inositol 1,4,5-Trisphosphate Receptors; D010833:Phytic Acid; D001397:Azoxymethane