Immunohistochemical characterization of mononuclear cells and MHC II expression in the brain of horses with experimental chronic Trypanosoma evansi infection.
An histochemical and immunohistochemical study was carried out to evaluate the mechanisms of immune response of horses experimentally infected by Trypanosoma evansi. For this purpose the HE histochemical stain and the avidin biotin peroxidase method were used. To determine the presence and immunoreactivity of immune cells we used anti-major histocompatibility complex II antibodies. Cellular infiltration phenotype was characterized with the aid of anti-CD3 antibody for T lymphocytes and by anti-BLA 36 antibodies for B lymphocytes. Macrophages were marked with an antibody against myeloid/histyocites antigen (clone Mac387). Lesions in the CNS of experimentally infected horses were those of a wide spread non suppurative encephalomyelitis and meningomyelitis. The severity of lesions varied in different parts of the nervous system, reflecting an irregular distribution of inflammatory vascular changes. Lymphoid perivascular cuffs and meningeal infiltrations were of predominantly composed of T and B cells. The parasite, T. evansi, was not identified in these horses tissues.
Lemos
Karen R
KR
Departamento de Medicina Veterinária, Centro Politécnico, UNICENTRO. Rua Simeão Camargo Varela de Sá, 03, Guarapuava, PR, 85040-080, Brasil. krlemos@yahoo.com.br
Marques
Luiz C
LC
Deaquino
Lúcia P C T
LP
Alessi
Antonio C
AC
Machado
Rozângela Z
RZ
D000818:Animals; D001921:Brain / Q000276:immunology* / Q000469:parasitology*; D002908:Chronic Disease; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses; D007150:Immunohistochemistry; D009000:Monocytes; D014352:Trypanosomiasis / Q000276:immunology / Q000662:veterinary*
D000949:Histocompatibility Antigens Class II