[Identification of proteins determining the virulence of Listeria monocytogenes using the bioinformation analysis].
The postgenomic stage of biotechnology allows the bioinformation approaches to be used for revealing previously unknown factors involved in different processes. In this work, bioinformation approaches were applied to analysis of factors involved in the L. monocytogenes virulence. Several open reading frames (ORFs) were identified in L. monocytogenes genome, which encode proteins with high level homology to bacterial peptidases and which meets the developed criteria. The influence of the ORFs on virulence was demonstrated by infection of mice with L. monocytogenes strains mutated in the studied genes. Growth rates, morphology, and production of surface proteins characterized the obtained strains.
Iurov
D S
DS
Pushkareva
V I
VI
Ermolaeva
S A
SA
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D005260:Female; D008089:Listeria monocytogenes / Q000235:genetics* / Q000472:pathogenicity*; D008088:Listeriosis / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D016366:Open Reading Frames / Q000235:genetics*; D010447:Peptide Hydrolases / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
D001426:Bacterial Proteins; D037521:Virulence Factors; D010447:Peptide Hydrolases