Effect of roughness of zirconia and titanium on fibroblast adhesion.
The aim of this study was to investigate the adhesion (4 and 24 h) and the morphology of fibroblast Balb/c 3T3 seeded onto polystyrene, partially stabilized (ZrO(2)Y(2)O(3)), stabilized zirconia ceramic (3YTZP), and pure titanium (Ti, grade 2). Initial cell adhesion (4 h) was greater (P < 0.05, analysis of variance and Tukey's Multiple Comparisons Test) onto ZrO(2)Y(2)O(3) and polystyrene than in Ti and 3YTZ. After 24 h, the number of adhered cells was similar between the biomaterials tested, but smaller than onto polystyrene (P < 0.05). Cells were more spread onto glass surface after 4 h, but after 24 h, the morphology and density of the cells were similar in all groups (SEM). Profilometry showed distinct Ra values for each material: glass coverslips and ZrO(2)Y(2)O(3) (0.09 microm), Ti (0.88 microm), and 3YTZP (30.93 microm). It was concluded that ZrO(2)Y(2)O(3) promoted the best initial adhesion, thus indicating that surfaces with Ra values smaller than 0.1 microm could be more favorable to initial adhesion.
Takamori
Esther Rieko
ER
Department of Biological Sciences, University of Sao Paulo, Bauru, São Paulo, Brazil.
Cruz
Renato
R
Gonçalvez
Fábio
F
Zanetti
Raquel Virgínia
RV
Zanetti
Artemio
A
Granjeiro
José Mauro
JM
D000818:Animals; D041702:BALB 3T3 Cells; D001672:Biocompatible Materials; D002448:Cell Adhesion; D048430:Cell Shape; D003776:Dental Porcelain / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology*; D051379:Mice; D011137:Polystyrenes / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
D001672:Biocompatible Materials; D011137:Polystyrenes; C499362:yttria stabilized tetragonal zirconia; D003776:Dental Porcelain; D015019:Yttrium; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide