title: Transcriptome profiling of Saccharomyces cerevisiae mutants lacking C2H2 zinc finger proteins.
abstract: BACKGROUND
  The budding yeast Saccharomyces cerevisiae is a eukaryotic organism with extensive genetic redundancy. Large-scale gene deletion analysis has shown that over 80% of the ~6200 predicted genes are nonessential and that the functions of 30% of all ORFs remain unclassified, implying that yeast cells can tolerate deletion of a substantial number of individual genes. For example, a class of zinc finger proteins containing C2H2 zinc fingers in tandem arrays of two or three is predicted to be transcription factors; however, seven of the thirty-one predicted genes of this class are nonessential, and their functions are poorly understood. In this study we completed a transcriptomic profiling of three mutants lacking C2H2 zinc finger proteins, ypr013cDelta,ypr015cDelta and ypr013cDeltaypr015cDelta.
  
  
  RESULTS
  Gene expression patterns were remarkably different between wild type and the mutants. The results indicate altered expression of 79 genes in ypr013cDelta, 185 genes in ypr015cDelta and 426 genes in the double mutant when compared with that of the wild type strain. More than 80% of the alterations in the double mutants were not observed in either one of the single deletion mutants. Functional categorization based on Munich Information Center for Protein Sequences (MIPS) revealed up-regulation of genes related to transcription and down-regulation of genes involving cell rescue and defense, suggesting a decreased response to stress conditions. Genes related to cell cycle and DNA processing whose expression was affected by single or double deletions were also identified.
  
  
  CONCLUSIONS
  Our results suggest that microarray analysis can define the biological roles of zinc finger proteins with unknown functions and identify target genes that are regulated by these putative transcriptional factors. These findings also suggest that both YPR013C and YPR015C have biological processes in common, in addition to their own regulatory pathways.
authors: Department of Biology, Tougaloo College, Tougaloo, MS 39174, USA. jmao@tougaloo.edu
authors: Jinghe
authors: J
authors: Mao
authors: Tanwir
authors: T
authors: Habib
authors: Ming
authors: M
authors: Shenwu
authors: Baobin
authors: B
authors: Kang
authors: Wilbur
authors: W
authors: Allen
authors: LaShonda
authors: L
authors: Robertson
authors: Jack Y
authors: JY
authors: Yang
authors: Youping
authors: Y
authors: Deng
mesh_terms: D004268:DNA-Binding Proteins / Q000172:deficiency* / Q000235:genetics; D020869:Gene Expression Profiling; D009154:Mutation / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins