Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptome.
Sperm analysis following World Health Organization guidelines is unable to explain the molecular causes of male infertility when basic sperm parameters are within a normal range and women do not present gynecologic pathology. Consequently, there is a need for accurate diagnostic tools in this area, and microarray technology emerges as promising. We present, for the first time, preliminary results of a comparison of sperm mRNA expression profiles between fertile and infertile men with normal semen parameters, discovering profound discrepancies between groups, with potential diagnostic and therapeutic possibilities.
Garrido
N
N
Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain. nicolas.garrido@ivi.es
Martínez-Conejero
J A
JA
Jauregui
J
J
Horcajadas
J A
JA
Simón
C
C
Remohí
J
J
Meseguer
M
M
D000951:Antigens, Neoplasm / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005298:Fertility / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D012661:Semen / Q000166:cytology / Q000378:metabolism; D013094:Spermatozoa / Q000002:abnormalities / Q000378:metabolism*; D014357:Trypsin; D014362:Trypsinogen / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000951:Antigens, Neoplasm; D051017:Apoptosis Regulatory Proteins; C534238:CAB39L protein, human; D012333:RNA, Messenger; D014362:Trypsinogen; D004247:DNA; D005723:gamma-Glutamyltransferase; C489303:PRSS1 protein, human; D014357:Trypsin