title: The carriage population of Staphylococcus aureus from Mali is composed of a combination of pandemic clones and the divergent Panton-Valentine leukocidin-positive genotype ST152.
abstract: Staphylococcus aureus is an important human pathogen, but it appears more commonly in asymptomatic colonization of the nasopharynx than in cases of invasive disease. Evidence concerning the global population structure of S. aureus is limited by the overrepresentation in the multilocus sequence testing database of disease isolates recovered from Western Europe, the Americas, Australia, and Japan. We address this by presenting data from the S. aureus carriage population in Mali, the first detailed characterization of asymptomatic carriage from an African population. These data confirm the pandemic spread of many of the common S. aureus clones in the carriage population. We also note the high frequency (approximately 24%) of a single divergent genotype, sequence type 152 (ST152), which has not previously been recovered from nasal carriage isolates but corresponds to a sporadic Panton-Valentine leukocidin (PVL)-positive, community-acquired methicillin-resistant S. aureus clone noted mostly in Central Europe. We show that 100% of the ST152 isolates recovered from nasal carriage samples in Mali are PVL positive and discuss implications relating to the emergence and spread of this virulent genotype.
authors: Hôpital Bichat-Claude Bernard, AP-HP, EA 3964, Université Denis Diderot-Paris 7 Medical School, 75870 Paris Cedex, France. raymond.ruimy@bch.ap-hop-paris.fr
authors: Raymond
authors: R
authors: Ruimy
authors: Aminata
authors: A
authors: Maiga
authors: Laurence
authors: L
authors: Armand-Lefevre
authors: Ibrahim
authors: I
authors: Maiga
authors: Amadou
authors: A
authors: Diallo
authors: Abdel Karim
authors: AK
authors: Koumaré
authors: Kalilou
authors: K
authors: Ouattara
authors: Sambou
authors: S
authors: Soumaré
authors: Kevin
authors: K
authors: Gaillard
authors: Jean-Christophe
authors: JC
authors: Lucet
authors: Antoine
authors: A
authors: Andremont
authors: Edward J
authors: EJ
authors: Feil
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001427:Bacterial Toxins / Q000235:genetics* / Q000378:metabolism; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology*; D004196:Disease Outbreaks; D005098:Exotoxins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D007956:Leukocidins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008302:Mali / Q000453:epidemiology; D008875:Middle Aged; D009666:Nose / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin