Pyrrhocoricin as a potential drug delivery vehicle for Cryptosporidium parvum.
This study analysed the intracellular delivery capacity of insect derived pyrrhocoricin with a peptide cargo in Cryptosporidium parvum in vitro using fluorescence microscopy. Results revealed that pyrrhocoricin was capable of acting as a delivery vehicle in transducing peptides across the parasite cell membrane for multiple life-cycle stages. The successful transduction may aid in target validation and the delivery of future peptide-based drugs against this important human pathogen.
Boxell
Annika
A
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
Lee
Stephanie Hui Chin
SH
Jefferies
Ryan
R
Watt
Paul
P
Hopkins
Richard
R
Reid
Simon
S
Armson
Anthony
A
Ryan
Una
U
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D000981:Antiprotozoal Agents / Q000008:administration & dosage*; D016785:Cryptosporidium parvum / Q000378:metabolism*; D004337:Drug Carriers; D019793:Fluorescein / Q000737:chemistry; D019476:Insect Proteins; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000008:administration & dosage*
D023181:Antimicrobial Cationic Peptides; D000981:Antiprotozoal Agents; D004337:Drug Carriers; D019476:Insect Proteins; D010455:Peptides; C087710:pyrrhocoricin protein, Pyrrhocoris apterus; D019793:Fluorescein