title: Cloning and characterization of an rRNA methyltransferase from Sorangium cellulosum.
abstract: A locus (kmr) responsible for aminoglycosides-resistance of Sorangium cellulosum was cloned and characterized in Myxococcus xanthus. The gene kmr encodes a putative rRNA methyltransferase. Expression of the complete ORF endowed the Myxococcus transformants with the resistance to aminoglycosidic antibiotics of kanamycin, apramycin, gentamycin, neomycin, and tobramycin at an extraordinary high-level (MIC, higher than 500 microg/ml). However, the gene did not function in Escherichia coli cells. In Sorangium genome, the gene kmr was followed by a putative integrase gene, and was highly homologous in different Sorangium strains. The Sorangium rRNA methyltransferase sequence was in low similarity to the reported 16S rRNA methyltransferases, and their resistance spectrums were also different. The results indicate that the rRNA methyltransferase (Kmr) in Sorangium strains is a new member of the rRNA methyltransferases family.
authors: State Key Laboratory of Microbial Technology, College of Life Science, Shandong University, Jinan 250100, People's Republic of China.
authors: Jing-Yi
authors: JY
authors: Zhao
authors: Zhi-Jie
authors: ZJ
authors: Xia
authors: Xia
authors: X
authors: Sun
authors: Li
authors: L
authors: Zhong
authors: De-Ming
authors: DM
authors: Jiang
authors: Hong
authors: H
authors: Liu
authors: Jing
authors: J
authors: Wang
authors: Zhong-Jun
authors: ZJ
authors: Qin
authors: Yue-Zhong
authors: YZ
authors: Li
mesh_terms: D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D007613:Kanamycin Resistance / Q000235:genetics*; D008780:Methyltransferases / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D009231:Myxococcales / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D016941:Myxococcus xanthus / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases