title: Cytokeratin 8 silencing in human nasopharyngeal carcinoma cells leads to cisplatin sensitization.
abstract: By comparing protein profiles of nasopharyngeal carcinoma HONE1 cells to transformed nasopharyngeal epithelial NP 69 cells, several clusters of differentially expressed proteins were identified. The increased expression of cytokeratin 8 (CK8) and pyruvate kinase M2 was a common feature in four NPC cell lines compared to the two transformed epithelial cell lines. Suppression of CK8 was associated with the sensitivity to cisplatin in HONE1 cells; while overexpression of CK8 provided resistance to cisplatin-mediated apoptosis; and this protection occurred through an enhanced phosphorylation of c-Jun NH(2)-terminal kinase (JNK). Our findings implicate an underlying molecular mechanism in which CK8 is required for cisplatin resistance.
authors: Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China.
authors: Ying
authors: Y
authors: Wang
authors: Qing-Yu
authors: QY
authors: He
authors: Sai-Wah
authors: SW
authors: Tsao
authors: Yim-Hing
authors: YH
authors: Cheung
authors: Anita
authors: A
authors: Wong
authors: Jen-Fu
authors: JF
authors: Chiu
mesh_terms: D017209:Apoptosis; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000502:physiology*; D020868:Gene Silencing; D006801:Humans; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D053533:Keratin-8 / Q000502:physiology*; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D010766:Phosphorylation; D040081:Protein Array Analysis; D014162:Transfection
chemical_list: D053533:Keratin-8; D048031:JNK Mitogen-Activated Protein Kinases; D002945:Cisplatin