Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera.
The western blot analysis for identification of immunogenic proteins in whole cell lysate (WCL) antigens (Ags) prepared from the Trypanosoma evansi of buffalo, horse and cattle origins using hyperimmune sera (HIS) showed 11 immunogenic proteins and naturally T. evansi infected immune sera (IS) of horse detected 19 immunogenic proteins. HIS and IS of horse recognized five common immunogenic proteins of relative molecular weight (M(r)) ranges 61-64, 44-47, 33-34, 25-26 and 14-16 kilo Dalton (kDa). HIS rose against WCL Ags of T. evansi of buffalo origin and immune sera of horse cross reacted with WCL Ags of T. evansi of different host origin. It can be concluded that in comparison to HIS, IS of horse could able to detect more numbers of immunogenic proteins and five common immunogenic proteins in WCL Ages of T. evansi of different hosts origin. The evidence of higher reactivity of IS in comparison to HIS against T. evansi is being reported for the first time.
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Indian Veterinary Research Institute, Kolkata 700 037, West Bengal, India.
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D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000276:immunology; D006734:Horse Diseases / Q000276:immunology / Q000469:parasitology*; D006736:Horses; D007194:India; D013045:Species Specificity; D014345:Trypanosoma / Q000276:immunology*; D014352:Trypanosomiasis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*
D000953:Antigens, Protozoan