Improved determination of quinolones in milk at their MRL levels using LC-UV, LC-FD, LC-MS and LC-MS/MS and validation in line with regulation 2002/657/EC.
This paper reports the multi-residue determination of quinolones included in the European Union regulations (marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin and flumequine) in bovine milk, using liquid chromatography with ultraviolet (LC-UV), fluorescence (LC-FD), mass spectrometry (LC-MS) and tandem mass spectrometry detection (LC-MS/MS). The methods were validated, in line with EU requirements (Commission Decision 2002/657/EC). Linearity, decision limit (CCalpha), detection capability (CCbeta), detection limit (LOD), quantification limits (LOQ), recoveries, precision, selectivity and stability were determined and adequate results were obtained. Recoveries higher than 80% were obtained for all quinolones. The methods developed were used to quantify enrofloxacin and its main metabolite, ciprofloxacin, in milk from animals treated with enrofloxacin.
Hermo
M P
MP
Barcelona University, Faculty of Chemistry, Department of Analytical Chemistry, Barcelona E-08028, Spain.
Nemutlu
E
E
Kir
S
S
Barrón
D
D
Barbosa
J
J
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D000077422:Enrofloxacin; D005062:European Union; D005453:Fluorescence; D024841:Fluoroquinolones / Q000737:chemistry; D016014:Linear Models; D008892:Milk / Q000737:chemistry*; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D014466:Ultraviolet Rays
D024841:Fluoroquinolones; D015363:Quinolones; D000077422:Enrofloxacin