title: Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
abstract: Vascular access dysfunction contributes to patient morbidity during maintenance hemodialysis. In this study we determined if knockout of heme oxygenase-1 predisposed to malfunction of arteriovenous fistulas. After three weeks, all fistulas in wild type mice were patent whereas a third of the fistulas in knockout mice were occluded and these exhibited increased neointimal hyperplasia and venous wall thickening. Heme oxygenase-1 mRNA and protein were robustly induced in the fistulas of the wild type mice. In the knockout mice there was increased PAI-1 and MCP-1 expression, marked induction of MMP-2 and MMP-9, but similar expression of PDGF alpha, IGF-1, TGF-beta1, VEGF, and osteopontin compared to wild type mice. We conclude that heme oxygenase-1 deficiency promotes vasculopathic gene expression, accelerates neointimal hyperplasia and impairs the function of arteriovenous fistulas.
authors: Division of Nephrology and Hypertension, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors: J P
authors: JP
authors: Juncos
authors: M J
authors: MJ
authors: Tracz
authors: A J
authors: AJ
authors: Croatt
authors: J P
authors: JP
authors: Grande
authors: A W
authors: AW
authors: Ackerman
authors: Z S
authors: ZS
authors: Katusic
authors: K A
authors: KA
authors: Nath
mesh_terms: D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D001808:Blood Vessels / Q000293:injuries / Q000473:pathology; D002408:Catheters, Indwelling / Q000009:adverse effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051547:Heme Oxygenase-1 / Q000172:deficiency* / Q000235:genetics / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D017539:Tunica Intima / Q000473:pathology
chemical_list: D051547:Heme Oxygenase-1