Two new bis-coumarin glycosides from Daphne giraldii NITSCHE.
Two new bis-coumarin glycosides were isolated from the stem barks of Daphne giraldii NITSCHE. Their structures were elucidated as 6-O-alpha-L-rhamnnopyranosyl daphnogirin (1), and 6-O-beta-D-apiofuranosyl daphnogirin (2), on the basis of detailed spectroscopic analysis. Compounds 1 and 2 were tested inhibitory effects against production of nitric oxide (NO) in RAW264.7 cells, and cytotoxicity of human tumor cell lines A549, LOVO, QGY-7703, 6T-CEM. The results showed that compounds 1 and 2 neither reduced production of NO, nor inhibited human tumor cell lines.
Su
Juan
J
Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai, PR China.
Wu
Zhi-Jun
ZJ
Zhang
Wei-Dong
WD
Zhang
Chuan
C
Li
Hui-Liang
HL
Liu
Run-Hui
RH
Shen
Yun-Heng
YH
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D003374:Coumarins / Q000737:chemistry* / Q000494:pharmacology*; D032364:Daphne / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D024301:Plant Bark / Q000737:chemistry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013778:Tetrazolium Salts; D013844:Thiazoles
C529362:6-O-alpha-L-rhamnnopyranosyl daphnogirin; C529363:6-O-beta-D-apiofuranosyl daphnogirin; D000972:Antineoplastic Agents, Phytogenic; D003374:Coumarins; D004791:Enzyme Inhibitors; D006027:Glycosides; D013778:Tetrazolium Salts; D013844:Thiazoles; D019001:Nitric Oxide Synthase; C022616:thiazolyl blue