Influence of hindlimb suspension on calcium-induced contraction characteristics in dystrophin-deficient animals.
The direct data concerning effects of unloading on dystrophic muscle were received in study of mdx mice, a model for Duchenne muscular dystrophy, muscles before and after hindlimb suspension. Experiments were performed on softer skinned soleus muscle fibers isolated from wild-type (C57black) as a control and mdx mice aged 2 weeks. Animals of two experimental groups were tail suspended during 21 days. In both groups of hindlimb suspended mice isolated soleus fibers were thinner than in the control groups. But there was a greater 37% significant decrease in fiber diameter in wild-type (CHS) suspended mice vs. 24% in mdx (MHS) suspended group. Values of absolute peak tension in CHS were less than in the control group by 33%, and in MHS mice suspended--by 39%. 21 days of hindlimb suspension resulted in reduction of mean peak specific tension by 28% in MHS and significantly less drop (15%) in CHS groups. We observed a similar rightward shift of the tension pCa curve in both mice strains.
Litvinova
K S
KS
RF SRC, Institute for Biomedical Problems of Russian Academy of Sciences, 76A, Khoroshevskoe shosse, 123007, Moscow, RF. karlit@bk.ru
Shenkman
B S
BS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004195:Disease Models, Animal; D016189:Dystrophin / Q000172:deficiency* / Q000235:genetics; D019417:Hindlimb Suspension; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009119:Muscle Contraction; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D053580:Muscle Strength; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
D016189:Dystrophin; D002118:Calcium