Subcellular localization of the Schlafen protein family.
Although the first members of the Schlafen gene family were first described almost 10 years ago, the precise molecular/biochemical functions of the proteins they encode still remain largely unknown. Roles in cell growth, haematopoietic cell differentiation, and T cell development/maturation have, with some experimental support, been postulated, but none have been conclusively verified. Here, we have determined the subcellular localization of Schlafens 1, 2, 4, 5, 8, and 9, representing all three of the murine subgroups. We show that the proteins from subgroups I and II localize to the cytoplasm, while the longer forms in subgroup III localize exclusively to the nuclear compartment. We also demonstrate upregulation of Schlafen2 upon differentiation of haematopoietic cells and show this endogenous protein localizes to the cytoplasm. Thus, we propose the different subgroups of Schlafen proteins are likely to have functionally distinct roles, reflecting their differing localizations within the cell.
Neumann
Brent
B
University of Queensland Diamantina Institute for Cancer, Immunology and Metabolic Medicine, Level 4, R-Wing, Building 1, Princess Alexandra Hospital, Brisbane, Qld. 4102, Australia.
Zhao
Liang
L
Murphy
Kathleen
K
Gonda
Thomas J
TJ
D000818:Animals; D018797:Cell Cycle Proteins / Q000032:analysis / Q000145:classification* / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D051379:Mice; D022423:Myeloid Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism; D010802:Phylogeny
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins