title: Characterization of tau in cerebrospinal fluid using mass spectrometry.
abstract: The neurodegenerative disorder Alzheimer's disease (AD) is the most common cause of dementia in the elderly. The presence of neurofibrillary tangles, consisting of hyperphosphorylated tau protein, is one of the major neuropathologic characteristics of the disease, making this protein an attractive biomarker for AD and a possible target for therapy. Here, we describe an optimized immunoprecipitation mass spectrometry method that enables, for the first time, detailed characterization of tau in human cerebrospinal fluid. The identities of putative tau fragments were confirmed using nanoflow liquid chromatography and tandem mass spectrometry. Nineteen tryptic fragments of tau were detected, of which 16 are found in all tau isoforms while 3 represented unique tau isoforms. These results pave the way for clinical CSF studies on the tauopathies.
authors: Clinical Neurochemistry Laboratory, Department of Neuroscience and Physiology, University of Göteborg, Sahlgrenska University Hospital, S-431 80 Mölndal, Sweden. erik.portelius@neuro.gu.se
authors: Erik
authors: E
authors: Portelius
authors: Sara F
authors: SF
authors: Hansson
authors: Ai Jun
authors: AJ
authors: Tran
authors: Henrik
authors: H
authors: Zetterberg
authors: Pierre
authors: P
authors: Grognet
authors: Eugeen
authors: E
authors: Vanmechelen
authors: Kina
authors: K
authors: Höglund
authors: Gunnar
authors: G
authors: Brinkmalm
authors: Ann
authors: A
authors: Westman-Brinkmalm
authors: Eckhard
authors: E
authors: Nordhoff
authors: Kaj
authors: K
authors: Blennow
authors: Johan
authors: J
authors: Gobom
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000595:Amino Acid Sequence; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000134:cerebrospinal fluid / Q000737:chemistry; D020033:Protein Isoforms / Q000134:cerebrospinal fluid* / Q000737:chemistry* / Q000235:genetics; D016875:tau Proteins / Q000134:cerebrospinal fluid* / Q000737:chemistry* / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D020033:Protein Isoforms; D016875:tau Proteins