Capillary zone electrophoresis method for the determination of famotidine and related impurities in pharmaceuticals.
A simple and rapid capillary zone electrophoresis (CZE) method with UV detection has been developed for the determination of famotidine and its potential impurities in pharmaceutical formulations. The electrophoretic separation of these compounds was performed using a fused silica capillary and 37.5mmolL(-1) phosphate buffer pH 3.5 as the electrolyte. Under the optimised conditions, six impurities could be resolved from the famotidine peak in less than 7min. The calibration curves obtained for the seven compounds were linear over the concentration range investigated (from 1.5 to 78.5microg mL(-1)). The intra- and inter-day relative standard deviations for the migration times and corrected peak areas were less than 2% and 5%, respectively. The detection limits were found to be 0.09microg mL(-1) for famotidine, and from 0.1 to 0.62microg mL(-1) depending on the impurities. The method has been successfully applied to the determination of famotidine in commercial dosage forms.
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Institut National des Sciences Appliquées et de Technologie, Laboratoire de Chimie Analytique et Electrochimie 04UR12-01, Centre Urbain Nord, B.P. No. 676, 1080 Tunis Cedex, Tunisia.
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Monser
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Taverna
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D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D015738:Famotidine / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015738:Famotidine