Characterisation of recombinant immunoreactive antigens of the scab mite Sarcoptes scabiei.
Sarcoptic mange (or scabies) is an important skin disease which can affect a variety of species including humans, cattle, goats, sheep, horses, pigs, rabbits, and dogs. Approximately 300 million people are affected worldwide and in lifestock animals the infestation may lead to substantial economic losses caused by depression in growth and feed conversion rates. Diagnosis of Sarcoptes infestation is difficult and only a few serological tests have been developed using whole mite antigen for diagnosis of mange in animals. Here we describe the isolation and characterisation of cDNAs of several immunoreactive clones and their recombinant expression in Escherichia coli. Three of the proteins contain repetitive sequences which suggests that they might be involved in immune evasion. The application of these antigens in serodiagnosis and the suitability for diagnosis is discussed.
Kuhn
C
C
Department of Molecular Parasitology, Institute of Biology, Humboldt-University Berlin, Berlin, Germany. Carola.Kuhn@rz.hu-berlin.de
Lucius
R
R
Matthes
H F
HF
Meusel
G
G
Reich
B
B
Kalinna
B H
BH
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D004926:Escherichia coli; D015723:Gene Library; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012520:Sarcoptes scabiei / Q000235:genetics* / Q000276:immunology*; D012532:Scabies / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment / Q000662:veterinary; D012698:Serologic Tests / Q000379:methods / Q000592:standards / Q000662:veterinary
D000941:Antigens; D018076:DNA, Complementary