title: Promoting the formation and stabilization of G-quadruplex by dinuclear RuII complex Ru2(obip)L4.
abstract: The remarkable ability of a new dinuclear Ru (II) complex Ru 2(obip)L 4 [obip = 2-(2-pyridyl)imidazo[4,5- f][1,10]-phenanthroline; L = 2,2'-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru 2(obip)L 4 could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive T m shifts of +9.4 and +5.8 degrees C in Na (+) and K (+) buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru 2(obip)L 4 at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru 2(obip)L 4-quadruplex interaction with a single guanine tetrad.
authors: Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, People's Republic of China.
authors: Shuo
authors: S
authors: Shi
authors: Jie
authors: J
authors: Liu
authors: Tianming
authors: T
authors: Yao
authors: Xiaoting
authors: X
authors: Geng
authors: Lingfeng
authors: L
authors: Jiang
authors: Qingyuan
authors: Q
authors: Yang
authors: Lin
authors: L
authors: Cheng
authors: Liangnian
authors: L
authors: Ji
mesh_terms: D015082:2,2'-Dipyridyl; D006801:Humans; D007093:Imidazoles; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010618:Phenanthrolines; D012428:Ruthenium; D016615:Telomere / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics
chemical_list: D007093:Imidazoles; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; D012428:Ruthenium