Biomolecules-carbon nanotubes doped conducting polymer nanocomposites and their sensor application.
A simple method for preparing bio-functionalized soluble single-walled carbon nanotubes (SWNTs) is described. Different proteins such as bovine serum albumin (BSA), cytochrome c and horseradish peroxidase (HRP) were used to solubilize low functionality SWNTs in water aided by sonication. The unbound proteins were removed by column chromatography and the SWNT-protein conjugate was used as the sole anionic dopant in electropolymerization of polypyrrole from polymerization solution at pH above the isoelectric point of the protein to provide a negative charge. The morphology of the polypyrrole with SWNT-protein dopant was found to be three-dimensional and fibrous with wide open interlocking pores in contrast to smooth and cauliflower-like for chloride doped polypyrrole. Enhanced sensor performance was demonstrated for hydrogen peroxide detection on polypyrrole/SWCNT-HRP nanocomposites modified electrode. Such nanocomposites can be potentially applied for other biosensor and bio-fuel cell applications.
Kum
Maxwell C
MC
Department of Chemical and Environmental Engineering, and Center for Nanoscale Science and Engineering, University of California, Riverside, CA 92521, USA.
Joshi
Kanchan A
KA
Chen
Wilfred
W
Myung
Nosang V
NV
Mulchandani
Ashok
A
D000042:Absorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D045304:Cytochromes c; D004563:Electrochemistry; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D012710:Serum Albumin, Bovine; D012995:Solubility
D037742:Nanotubes, Carbon; D011108:Polymers; D011506:Proteins; D011758:Pyrroles; D012710:Serum Albumin, Bovine; C067635:polypyrrole; D045304:Cytochromes c; D006735:Horseradish Peroxidase