Reverse flow injection spectrophotometric determination of iron(III) using chlortetracycline reagent.
A simple reversed flow injection colourimetric procedure for determining iron(III) was proposed. It is based on the reaction between iron(III) with chlortetracycline, resulting in an intense yellow complex with a suitable absorption at 435nm. A 200mul chlortetracycline reagent solution was injected into the phosphate buffer stream (flow rate 2.0mlmin(-1)) which was then merged with iron(III) standard or sample in dilute nitric acid stream (flow rate 1.5mlmin(-1)). Optimum conditions for determining iron(III) were investigated by univariate method. Under the optimum conditions, a linear calibration graph was obtained over the range 0.5-20.0mugml(-1). The detection limit (3sigma) and the quantification limit (10sigma) were 0.10 and 0.82mugml(-1), respectively. The relatives standard deviation of the proposed method calculated from 12 replicate injections of 2.0 and 10.0mugml(-1) iron(III) were 0.43 and 0.59%, respectively. The sample throughput was 60h(-1). The proposed method has been satisfactorily applied to the determination of iron(III) in natural waters.
Ruengsitagoon
Wirat
W
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
D002138:Calibration; D002751:Chlortetracycline; D003124:Colorimetry; D017022:Flow Injection Analysis; D005618:Fresh Water / Q000737:chemistry; D007202:Indicators and Reagents; D007501:Iron / Q000032:analysis*; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
D007202:Indicators and Reagents; D007501:Iron; D002751:Chlortetracycline