Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection.
We admixed cultured porcine keratocytes or corneal endothelial cells in the presence of human sera or peripheral blood mononuclear cells (PBMCs) for 4 to 72 hours to investigate their immune-related susceptibilities to xeno-related rejection. We evaluated complement deposition at 48 hours by flow cytometry after staining with the C3 anti-goat cy3 antibody. The inhibition of proliferation of porcine corneal cells by human sera was examined using the 3-[4,5-dimethy/thiazol-2,5-dephenyl tetrazolium bromide (MTT) assay over 24 to 72 hours. The amount of 51chromium (Cr)-release was estimated after a reaction between the porcine cells and human PBMCs for 4 hours. There was greater C3 deposition in keratocytes (60.2%) than in endothelial cells (26.9%; P = .05, Mann-Whitney U test). Both keratocytes and endothelial cells showed significant levels of proliferative inhibition over a period of 72 hours. The number of 51Cr-release cells on interleukin-2 addition was significantly higher among keratocytes (88.0%) than endothelial cells (51.4%) at a 1:100 target:effector ratio (P = .04, Mann-Whitney U test). Our present data suggested that porcine keratocytes might be key target cells in xeno-related rejections when the porcine cornea is transplanted to primates.
Kim
M K
MK
Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
Oh
J Y
JY
Lee
H I
HI
Ko
J H
JH
Lee
H J
HJ
Lee
J H
JH
Wee
W R
WR
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology* / Q000276:immunology*; D016039:Corneal Transplantation / Q000276:immunology*; D017931:DNA Primers; D042783:Endothelial Cells / Q000166:cytology* / Q000276:immunology*; D016232:Endothelins / Q000235:genetics; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D014183:Transplantation, Heterologous / Q000276:immunology* / Q000473:pathology; D014746:Vimentin / Q000235:genetics
D017931:DNA Primers; D016232:Endothelins; D014746:Vimentin