[Construction of CyclinD (-/-)/Patched (+/-) double knockout mice].
OBJECTIVE
To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways.


METHODS
Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/-), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice.


RESULTS
CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained.


CONCLUSIONS
The method of amphimixis can construct CycD (--)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.
Xin
Shi-Jie
SJ
Department of Vascular Surgery, First Hospital of China Medical University, Shenyang 110001, China. xinshijie1963@yahoo.com.cn
Li
Xuan
X
Molly
Duman-Scheel
DS
Wei
Du
D
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D056741:Cyclin D; D016213:Cyclins / Q000235:genetics* / Q000502:physiology; D005260:Female; D053823:Hedgehog Proteins / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000502:physiology; D016160:Retinoblastoma Protein / Q000502:physiology; D015398:Signal Transduction
D056741:Cyclin D; D016213:Cyclins; D053823:Hedgehog Proteins; D000072079:Patched Receptors; D000072081:Patched-1 Receptor; C000606240:Ptch1 protein, mouse; D011956:Receptors, Cell Surface; D016160:Retinoblastoma Protein