title: N-hexanoyl chitosan-stabilized magnetic nanoparticles: enhancement of adenoviral-mediated gene expression both in vitro and in vivo.
abstract: We developed hexanoyl chloride-modified chitosan (Nac-6) stabilized iron oxide nanoparticles (Nac-6-IOPs) as magnetic nanoparticles for viral gene (Ad/LacZ) delivery via magnetofection. This vector, Nac-6-IOPs/Ad/LacZ, binds to K562 cells in the presence of external magnetic fields and results in enhanced expression of the transgene in those cells that do not exhibit the coxsackie-adenovirus receptor (CAR). Our results demonstrate that Nac-6-IOPs/Ad/LacZ is able to transduce K562 cells specifically with reduced infection of CAR- cells. The dramatic enhancement in intracellular trafficking of the adenovirus without genetically modified vesicles can lead to enhanced nuclear transfer, especially in CAR- cells. In vivo magnetofection results also clearly demonstrated that the present Nac-6-IOPs could be applied in other cell lines. Whether cells or organs, in the presence of magnetic fields Nac-6-IOPs/Ad/LacZ has high transduction efficiency. The newly formulated Nac-6-IOPs, introduced by magnetofection, provide a high-throughput gene screening both in vitro and in vivo.
authors: Department of Pharmaceutical Sciences, Wayne State University, Detroit, Michigan, USA.
authors: Shanta Raj
authors: SR
authors: Bhattarai
authors: Sun Young
authors: SY
authors: Kim
authors: Kyu Yun
authors: KY
authors: Jang
authors: Ki Chang
authors: KC
authors: Lee
authors: Ho Keun
authors: HK
authors: Yi
authors: Dae Yeol
authors: DY
authors: Lee
authors: Hak Yong
authors: HY
authors: Kim
authors: Pyoung Han
authors: PH
authors: Hwang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D004279:DNA, Viral / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D020014:K562 Cells; D008280:Magnetics; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D004279:DNA, Viral; D004337:Drug Carriers; D048271:Chitosan