title: Water extract of Cordyceps sinensis (WECS) inhibits the RANKL-induced osteoclast differentiation.
abstract: It has been reported that Cordyceps sinensis, a traditional Chinese medicine, has various pharmacological effects. The aim of this study was to clarify the effect of water extract of Cordyceps sinensis (WECS) on osteoclast differentiation in vitro. In mouse bone marrow cells and monocyte/macrophage cell line RAW264.7, WECS dose-dependently inhibited the receptor activator of nuclear factor kappa B (NF-kappaB) ligand (RANKL)-induced osteoclast differentiation by tartrate-resistant acid phosphatase (TRAP) staining. In fact, cytotoxic effect was not observed in the RAW264.7 cells treated with WECS. Moreover, the mRNA expression of osteoclast related genes (calcitonin receptor, cathepsin K, matrix metalloprotease 9 and nuclear factor of activated T cells c1) was also inhibited by WECS. Investigation of inhibitory mechanism by using electrophoretic mobility shift assay (EMSA) and Western blot analysis revealed that WECS inhibited the activation of NF-kappaB through the prevention of IkappaBalpha phosphorylation. In conclusion, the present results demonstrate for the first time that WECS is a potent inhibitor of the RANKL-induced osteoclast differentiation through a mechanism involving the NF-kappaB pathway.
authors: Department of Clinical Nutrition, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto-cho, Tokushima, Japan.
authors: Yoko
authors: Y
authors: Mizuha
authors: Hironori
authors: H
authors: Yamamoto
authors: Tadatoshi
authors: T
authors: Sato
authors: Mitsuyoshi
authors: M
authors: Tsuji
authors: Masashi
authors: M
authors: Masuda
authors: Masayuki
authors: M
authors: Uchida
authors: Kentaro
authors: K
authors: Sakai
authors: Yutaka
authors: Y
authors: Taketani
authors: Koji
authors: K
authors: Yasutomo
authors: Hajime
authors: H
authors: Sasaki
authors: Eiji
authors: E
authors: Takeda
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D056657:Cathepsin K; D002403:Cathepsins / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D032661:Cordyceps / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000187:drug effects; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D050778:NFATC Transcription Factors / Q000235:genetics; D010010:Osteoclasts / Q000187:drug effects*; D053245:RANK Ligand / Q000494:pharmacology*; D018003:Receptors, Calcitonin / Q000235:genetics
chemical_list: D016328:NF-kappa B; D050778:NFATC Transcription Factors; D053245:RANK Ligand; D018003:Receptors, Calcitonin; D002403:Cathepsins; D056657:Cathepsin K; C534132:Ctsk protein, mouse; D020780:Matrix Metalloproteinase 9