[Comparative study of the resistance of bacteriophageT4, PhiX174D, MS2 and f2 to gamma radiation].
OBJECTIVE
To screen the suitable bacteriophage as virus indicator in irradiation sterilization.


METHODS
Suspensions of bacteriophage T4, phiX174D, MS2, and f2, Escherichia coli 8099, and Bacillus subtilis var.niger.sp. ATCC9372 were irradiated with (60)Co-gamma ray. The mean log(10) inactivation value (LIV) and killing log value (KL) were calculated.


RESULTS
(1) Under 100 Gy of gamma-radiation, the LIV levels of the bacteriophage T4, PhiX174, f2, and MS2 were 6.31, 6.92, 5.74, and 4.46 log(10) respectively, all reaching the disinfection level (LIV >/= 4.00 log(10)), (2) Under the same absorbed dose, the KL of Escherichia coli 8099 was > 7.97 log(10); (3) Under the same absorbed dose, the KL of the Bacillus subtilis var.niger.sp. ATCC9372 was 1.61 log(10).


CONCLUSIONS
The order of resistance of the above six microorganisms to gamma-radiation from the biggest to the smallest is as follows: Bacillus subtilis var. niger. sp. > bacteriophage MS2 > bacteriophage f2 > bacteriophage T4 > bacteriophage phiX 174D > E. coli.
Chen
Zhao-Bin
ZB
Shenzhen Nanshan Center for Disease Prevention and Control, Shenzhen 518054, China.
Huang
Hong-Ying
HY
Zhang
Chao-Wu
CW
D001412:Bacillus subtilis / Q000254:growth & development / Q000528:radiation effects; D017122:Bacteriophage T4 / Q000254:growth & development / Q000528:radiation effects*; D010584:Bacteriophage phi X 174 / Q000254:growth & development / Q000528:radiation effects*; D001435:Bacteriophages / Q000254:growth & development / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000254:growth & development / Q000528:radiation effects; D005720:Gamma Rays