title: Analysis of solvent content and oligomeric states in protein crystals--does symmetry matter?
abstract: A nonredundant set of 9081 protein crystal structures in the Protein Data Bank was used to examine the solvent content, the number of polypeptide chains, and the oligomeric states of proteins in crystals as a function of crystal symmetry (as classified by crystal systems and space groups). It was found that there is a correlation between solvent content and crystal symmetry. Surprisingly, proteins crystallizing in lower symmetry systems have lower solvent content compared to those crystallizing in higher symmetry systems. Nevertheless, there is no universal correlation between solvent content and preferences of macromolecules to crystallize in certain space groups. Crystal symmetry as a function of oligomeric state was examined, where trimers, tetramers, and hexamers were found to prefer to crystallize in systems where the oligomer symmetry could be incorporated in the crystal symmetry. Our analysis also shows that the frequency distribution within the enantiomorphous pairs of space groups does not differ significantly, in contrast to previous reports.
authors: Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA.
authors: Maksymilian
authors: M
authors: Chruszcz
authors: Wojciech
authors: W
authors: Potrzebowski
authors: Matthew D
authors: MD
authors: Zimmerman
authors: Marek
authors: M
authors: Grabowski
authors: Heping
authors: H
authors: Zheng
authors: Piotr
authors: P
authors: Lasota
authors: Wladek
authors: W
authors: Minor
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D008962:Models, Theoretical; D020836:Protein Structure, Quaternary; D011506:Proteins / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D011506:Proteins; D012997:Solvents