Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus bacteria in environmental waters.
An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.
Morris
Ceri A
CA
MLT Research Ltd., 5 Chiltern Close, Cardiff CF14 5DL, United Kingdom. ceri.morris@mltresearch.com
Blackwood
A Denene
AD
Kirs
Marek
M
Buttigieg
Neil D
ND
Morgan
Rhian R
RR
Hogan
James J
JJ
Weeks
Ian
I
Noble
Rachel T
RT
D001431:Bacteriological Techniques / Q000379:methods*; D016983:Enterococcus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014871:Water Microbiology