Direct determination of serotonin in gut lavage fluid by liquid chromatographic ion trap tandem mass spectrometry.
Direct determination of serotonin (5-HT) in gut lavage fluid from patients examined due to various gastrointestinal complaints has been achieved. The method involves addition of 5-methoxytryptamine (5-CH3O-HT) internal standard, centrifugation, filtration and injection of the sample supernatant in a liquid chromatographic system coupled to an ion trap tandem mass detector. Electro-spray in positive mode was used to isolate and fragment the protonated ions [5-HT+H]+ and [5-CH3O-HT+H]+ signals 177 and 191 m/z, respectively. Quantification was carried out by extracting the ion fragment chromatograms at 160 and 174 m/z for 5-HT and 5-CH3O-HT, respectively. The relationship 5-HT/5-CH3O-HT was modelled by using a simultaneous design in order to estimate the optimal amount of internal standard to be added to the samples prior to quantification.
Gregersen
Kine
K
National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 2029, Nordnes, N-5817 Bergen, Norway.
Frøyland
Livar
L
Berstad
Arnold
A
Araujo
Pedro
P
D034841:Abdominal Cavity; D001826:Body Fluids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D012701:Serotonin / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D007507:Therapeutic Irrigation
D012701:Serotonin