title: Physicochemical properties of fluorescent probes: experimental and computational determination of the overlapping pKa values of carboxyfluorescein.
abstract: The overlapping pKa values of carboxyfluorescein have been measured by applying a UV-vis spectroscopic method that uses absorbance diagrams and chemometric analysis. Quantum chemical calculations have also been performed in order to analyze the experimental data.
authors: Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università degli Studi de L'Aquila, Via Vetoio (Coppito II), 67010 Coppito, L'Aquila, Italy.
authors: Massimiliano
authors: M
authors: Aschi
authors: Angelo A
authors: AA
authors: D'Archivio
authors: Antonella
authors: A
authors: Fontana
authors: Alessandra
authors: A
authors: Formiglio
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003198:Computer Simulation; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D015394:Molecular Structure; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D007477:Ions; D014867:Water; C024098:6-carboxyfluorescein