Geminivirus: biolistic inoculation and molecular diagnosis.
The Geminiviridae family is a large family of plant viruses that has single-stranded DNA genomes and infects a large variety of crop species. In this chapter, we describe a biolistic inoculation protocol that has been successfully used to propagate new species of geminivirus in permissive hosts with total DNA extracted from infected plants. This allows us to directly investigate the biological properties of uncloned and not sap-transmissible geminiviruses.
Santos
Anésia A
AA
Departamento de Bioquímica e Biologia Molecular, BIOAGRO- Universidade Federal de Viçosa-36571.000, Viçosa, MG, Brazil.
Florentino
Lilian H
LH
Pires
Acássia B L
AB
Fontes
Elizabeth P B
EP
D019470:Biolistics / Q000379:methods*; D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity*; D018745:Genome, Plant; D016679:Genome, Viral; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods
D018744:DNA, Plant; D004277:DNA, Single-Stranded; D004279:DNA, Viral