title: p16(INK4a) translation suppressed by miR-24.
abstract: BACKGROUND
  Expression of the tumor suppressor p16(INK4a) increases during aging and replicative senescence.
  
  
  RESULTS
  Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decreased levels of miR-24, a microRNA that was predicted to associate with the p16 mRNA coding and 3'-untranslated regions. Ectopic miR-24 overexpression reduced p16 protein but not p16 mRNA levels. Conversely, introduction of antisense (AS)-miR-24 blocked miR-24 expression and markedly enhanced p16 protein levels, p16 translation, and the production of EGFP-p16 reporter bearing the miR-24 target recognition sites.
  
  
  CONCLUSIONS
  Together, our results suggest that miR-24 represses the initiation and elongation phases of p16 translation.
authors: Laboratory of Cellular and Molecular Biology, National Institute on Aging-IRP, National Institutes of Health, Baltimore, Maryland, United States of America. alal@cbrinstitute.org
authors: Ashish
authors: A
authors: Lal
authors: Hyeon Ho
authors: HH
authors: Kim
authors: Kotb
authors: K
authors: Abdelmohsen
authors: Yuki
authors: Y
authors: Kuwano
authors: Rudolf
authors: R
authors: Pullmann
authors: Subramanya
authors: S
authors: Srikantan
authors: Ramesh
authors: R
authors: Subrahmanyam
authors: Jennifer L
authors: JL
authors: Martindale
authors: Xiaoling
authors: X
authors: Yang
authors: Fariyal
authors: F
authors: Ahmed
authors: Francisco
authors: F
authors: Navarro
authors: Derek
authors: D
authors: Dykxhoorn
authors: Judy
authors: J
authors: Lieberman
authors: Myriam
authors: M
authors: Gorospe
mesh_terms: D001483:Base Sequence; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D017931:DNA Primers; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D014176:Protein Biosynthesis
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D017931:DNA Primers; D035683:MicroRNAs