title: Coenzyme F420-dependent sulfite reductase-enabled sulfite detoxification and use of sulfite as a sole sulfur source by Methanococcus maripaludis.
abstract: Coenzyme F(420)-dependent sulfite reductase (Fsr) of Methanocaldococcus jannaschii, a sulfite-tolerant methanogen, was expressed with activity in Methanococcus maripaludis, a sulfite-sensitive methanogen. The recombinant organism reduced sulfite to sulfide and grew with sulfite as the sole sulfur source, indicating that Fsr is a sulfite detoxification and assimilation enzyme for methanogens and that M. maripaludis synthesizes siroheme.
authors: Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors: Eric F
authors: EF
authors: Johnson
authors: Biswarup
authors: B
authors: Mukhopadhyay
mesh_terms: D003001:Cloning, Molecular; D015870:Gene Expression; D017015:Methanococcales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics / Q000378:metabolism*; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism; D013447:Sulfites / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism
chemical_list: D013440:Sulfides; D013447:Sulfites; C015084:coenzyme F420; D013455:Sulfur; D050862:Oxidoreductases Acting on Sulfur Group Donors; D012256:Riboflavin