Polyacetylenes from the roots of cultivated-wild ginseng and their cytotoxicity in vitro.
Column chromatographic separation of the roots of cultivated-wild ginseng (Jangnoisam) led to the isolation of seven polyacetylenes (1-7). Their structures were determined by spectroscopic methods to be panaxynol (1), ginsenoyne-A (2), panaxydol (3), 10-methoxy heptadeca-1-ene-4, 6-dyne-3, 9-diol (4) (3R, 9R, 10R)-panaxytriol (5), panaxyne (6), and ginsenoyne-C (7). These compounds were isolated from this source for the first time. The compounds were tested for their cytotoxic activity against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15) in vitro using the SRB method. Panaxydol (3) and panaxyne (6) showed significant and selective cytotoxicity against SK-OV-3 with ED50 values 2.93 and 1.40 microM, respectively.
Yang
Min Cheol
MC
Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
Seo
Dong Sang
DS
Choi
Sang Un
SU
Park
Young Hyun
YH
Lee
Kang Ro
KR
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004396:Coloring Agents; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D053279:Polyynes / Q000302:isolation & purification* / Q000494:pharmacology*; D012235:Rhodamines; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D012235:Rhodamines; D053279:Polyynes; C022027:lissamine rhodamine B