Identification of gender-regulated genes in Ancylostoma braziliense by real-time RT-PCR.
Ancylostoma braziliense belongs to the family Ancylostomatidae and infects cats and dogs in various parts of the tropical world. It is also a zoonotic parasite causing cutaneous larva migrants in humans. There are very few, either biological or molecular, studies of this species. In this study, differential display was used to identify differentially expressed genes in male and female A. braziliense. Nineteen new sequences were identified and examined by real-time RT-PCR to confirm male-female specificity. Ten were more expressed in males, while two were more expressed in females. Molecules shown to be important in other host-parasite relationships were also found in this study.
Costa
Ana Flávia D V
AF
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Campus Pampulha, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil.
Gomes-Ruiz
Alessandra C
AC
Rabelo
Elida M L
EM
D000722:Ancylostoma / Q000235:genetics*; D000818:Animals; D002415:Cats; D018076:DNA, Complementary; D004285:Dogs; D020224:Expressed Sequence Tags; D005260:Female; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions; D006801:Humans; D007815:Larva Migrans / Q000469:parasitology / Q000662:veterinary; D008297:Male; D018510:RNA, Helminth / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012733:Sex Differentiation
D018076:DNA, Complementary; D018510:RNA, Helminth