Biodegradable nanoparticles of partially methylated fungal poly(beta-L-malic acid) as a novel protein delivery carrier.
The preparation of nanoparticles from 75% methylated poly(beta-L-malic acid) is described. Their degradation in aqueous environments was examined and the influence of pH and lipase on the rate of hydrolysis was evaluated. Six proteins were used to estimate the loading efficiency of the nanoparticles. The amount of protein retained in the nanoparticles was found to depend on the acid/basic character of the protein. Protein release from the loaded nanoparticles upon incubation in water under physiological conditions encompassed polymer hydrolysis and happened steadily within 3-10 d. The activity loss of entrapped alpha-chymotrypsin caused by loading and releasing depended on the method used for loading.
Portilla-Arias
José A
JA
Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, ETSEIB, Diagonal 647, 08028 Barcelona, Spain.
García-Alvarez
Montserrat
M
Galbis
Juan A
JA
Muñoz-Guerra
Sebastián
S
D000818:Animals; D002234:Carbodiimides / Q000737:chemistry; D002918:Chymotrypsin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D045304:Cytochromes c / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004951:Esterification; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007782:Lactoglobulins / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D008049:Lipase; D008293:Malates / Q000138:chemical synthesis / Q000737:chemistry*; D008745:Methylation; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D009211:Myoglobin / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D016839:Physarum polycephalum / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011506:Proteins / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D055672:Static Electricity; D013499:Surface Properties
D002234:Carbodiimides; D004337:Drug Carriers; D007782:Lactoglobulins; D008293:Malates; D009211:Myoglobin; D011108:Polymers; D011506:Proteins; D012710:Serum Albumin, Bovine; C074640:poly(malate); D045304:Cytochromes c; D008049:Lipase; D009113:Muramidase; D002918:Chymotrypsin; C117608:alpha-chymotrypsin