trans,trans-2,4-decadienal induces mitochondrial dysfunction and oxidative stress.
Lipid peroxidation produces a large number of reactive aldehydes as secondary products. We have previously shown that the reaction of cytochrome c with trans,trans-2,4-decadienal (DDE), an aldehyde generated as a product of lipid peroxidation in cell membranes, results in the formation of adducts. Mass spectrometry analysis indicated that His-33, Lys-39, Lys-72 and Lys-100 in cytochrome c were modified by DDE. In the present work, we investigated the effect of DDE on isolated rat liver mitochondria. DDE (162 microM) treatment increases the rate of mitochondrial oxygen consumption. Extensive mitochondrial swelling upon treatment with DDE (900 nM-162 microM) was observed by light scattering and transmission electron microscopy experiments. DDE-induced loss of inner mitochondrial membrane potentials, monitored by safranin O fluorescence, was also observed. Furthermore, DDE-treated mitochondria showed an increase in lipid peroxidation, as monitored by MDA formation. These results suggest that reactive aldehydes promote mitochondrial dysfunction.
Sigolo
Carlos A O
CA
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, CEP 05508-900, São Paulo, Brazil.
Di Mascio
Paolo
P
Kowaltowski
Alicia J
AJ
Garcia
Camila C M
CC
Medeiros
Marisa H G
MH
D000818:Animals; D002478:Cells, Cultured; D003633:Dichlorodiphenyl Dichloroethylene / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000502:physiology*; D018384:Oxidative Stress / Q000528:radiation effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
D003633:Dichlorodiphenyl Dichloroethylene