title: Effect of periodontal treatment on IL-1beta, IL-1ra, and IL-10 levels in gingival crevicular fluid in patients with aggressive periodontitis.
abstract: OBJECTIVE
  The aim of this study was to examine the effect of phase I periodontal treatment on the levels of interleukin (IL)-1beta, IL-1ra, and IL-10 in gingival crevicular fluid (GCF) in patients with generalized aggressive periodontitis (G-AgP).
  
  
  METHODS
  Data were obtained from 15 patients with aggressive periodontitis and 15 healthy controls. GCF was collected from at least four pre-selected sites (one shallow, at least two moderate, or at least one deep pockets) in patients with G-AgP. In the healthy group, GCF samples were collected from one site. The cytokine levels were determined by an enzyme-linked immunosorbent assay. Probing depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. The GCF sampling and clinical measurements were recorded at baseline and 6 weeks later after periodontal treatment.
  
  
  RESULTS
  IL-1beta levels were significantly higher at the moderate and deep pocket sites compared with the shallow sites (p<0.05). After periodontal therapy, IL-1beta levels were significantly reduced in the moderate and deep pocket sites (p<0.05). IL-1ra levels at baseline of the moderate and deep pocket sites were significantly lower than the control sites (p<0.05). IL-10 levels were similar in all pockets and did not change after periodontal therapy.
  
  
  CONCLUSIONS
  The periodontal treatment improves the clinical parameters in G-AgP, and this improvement is evident in deep pocket sites for pocket depth and CAL values. These results confirm that IL-1beta is effective for evaluating the periodontal inflammation and can thus be used as a laboratory tool for assessing the activity of periodontal disease.
authors: Department of Periodontology, Cumhuriyet University Faculty of Medicine, Sivas, Turkey. hcakmak@cumhuriyet.edu.tr
authors: Hulya
authors: H
authors: Toker
authors: Omer
authors: O
authors: Poyraz
authors: Kaya
authors: K
authors: Eren
mesh_terms: D000208:Acute Disease; D000328:Adult; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D012534:Dental Scaling; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000096:biosynthesis*; D016753:Interleukin-10 / Q000096:biosynthesis*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008297:Male; D010518:Periodontitis / Q000378:metabolism / Q000628:therapy*; D018709:Statistics, Nonparametric
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D053583:Interleukin-1beta; D016753:Interleukin-10