Oxybutynin effects on Staphylococcus epidermidis biofilm production.
BACKGROUND
Oxybutynin is a tertiary amine and has chemical similarities like protamine sulfate. Protamine sulfate's effect on bacterial viability has been shown in some studies; however, there is scanty data regarding the effect of oxybutynin on bacterial viability. Accordingly, the aim of the present study was to investigate the effect of oxybutynin on bacterial viability.


METHODS
Twenty of Staphylococcus epidermidis (S. epidermidis) strains were isolated from patients who had catheter-associated urinary tract infection. These strains were exposed to 5 different concentrations of oxybutynin in tryptic soy broth (TSB) media (max: 5 mg/mL, min: 0.05 mg/mL). Quantitative growth patterns were measured by spectrophotometer. Under the same media conditions, biofilm production of individual strains was measured by Deighton's micromethod at the end of the 6-hour incubation period.


RESULTS
After the incubation period, weak and strong slime-producing bacteria groups were obtained and both groups' slime productions were extensively low at 5 mg/mL concentration of oxybutynin. Slime production of S. epidermidis was inversely correlated with oxybutynin concentrations.


CONCLUSIONS
Although there was a dose-dependent reduction of biofilm production, there seemed to be no bactericidal effect of oxybutynin on S. epidermidis.
Gedik
Abdullah
A
Department of Urology, Diyarbakir Hospital, Urology Clinic, Diyarbakir, Turkey.
Ersay
Ahmet
A
Atmaca
Selahattin
S
Sener
Alper
A
D018441:Biofilms / Q000187:drug effects*; D002408:Catheters, Indwelling / Q000009:adverse effects / Q000382:microbiology*; D018678:Cholinergic Agents / Q000494:pharmacology*; D006801:Humans; D008333:Mandelic Acids / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development; D014552:Urinary Tract Infections / Q000382:microbiology
D018678:Cholinergic Agents; D008333:Mandelic Acids; C005419:oxybutynin