Temporal study of estrogenic responses of mussel (Mytilus galloprovinciallis) extracts applied to reporter cell lines.
In vitro bioassays would facilitate monitoring of estrogen-like compounds in mussels (Mytilus galloprovincialis) since they tend to accumulate lipophilic compounds in their fat and muscle tissue. However, estradiol (E2) steroid (already identified in mussels) could induce false positive responses. This study focuses on temporal variability in estrogenic responses of mussel extracts and the possible relation of this response with E2 levels. The reproductive cycle and growth were thus monitored for six months in sexed mussels. E2 levels were similar between genders and these levels varied with reproductive stages. human estrogen receptor (hER) activities were similar at all stages except in February. E2 present in male and female extracts accounted for part of the hER activation observed, but no correlation was found between E2 levels and hER activities.
David
Arthur
A
Hydrosciences, UMR 5559-Faculté de Pharmacie Avenue Charles Flahaut, F-34093 Montpellier Cedex 5, BP 14491, France.
Dagnino
Sonia
S
Pichot
Yves
Y
Munaron
Dominique
D
Escande
Aurélie
A
Casellas
Claude
C
Fenet
Hélène
H
Gomez
Elena
E
D000818:Animals; D002460:Cell Line; D004958:Estradiol / Q000378:metabolism*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D017930:Genes, Reporter / Q000235:genetics; D008297:Male; D049878:Mytilus / Q000737:chemistry* / Q000502:physiology; D013997:Time Factors
C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D004958:Estradiol