title: Preventive effects of curcumin and dexamethasone on lung transplantation-associated lung injury in rats.
abstract: OBJECTIVE
  To investigate potential effects of curcumin or dexamethasone on lung transplantation-associated lung injury.
  
  
  METHODS
  Prospective, randomized, controlled study.
  
  
  METHODS
  Research laboratory.
  
  
  METHODS
  Adult male Sprague-Dawley rats.
  
  
  METHODS
  Sham-operated rats were used as time-matched controls. Experimental rats were subjected to unilateral orthotopic lung transplantation with 4 hrs of cold ischemia followed by 2 hrs (or 24 hrs) of reperfusion. Animals were randomly assigned to vehicle-, curcumin-, or dexamethasone-treated groups.
  
  
  RESULTS
  Transplantation-associated lung injury was characterized by an increased alveolar-capillary permeability and myeloperoxidase activity and decreased levels of arterial oxygen tension/inspired oxygen concentration ratio. Pretreatment with curcumin and dexamethasone significantly prevented barrier disruption, lung edema, tissue inflammation, and decreased PaO2 at the early stage of posttransplantation. Nuclear factor-kappaB in transplanted lungs was activated, accompanied by an increase in messenger RNA levels and protein content of tumor necrosis factor-alpha, interleukin-6, and matrix metalloproteinase-9 in lung graft. Those changes were prevented by pretreatment with curcumin and dexamethasone.
  
  
  CONCLUSIONS
  Curcumin can be an alternative therapy for protecting lung transplantation-associated injury by suppressing nuclear factor-kappaB-mediated expression of inflammatory genes.
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, PR China.
authors: Jiayuan
authors: J
authors: Sun
authors: Weigang
authors: W
authors: Guo
authors: Yong
authors: Y
authors: Ben
authors: Jinjun
authors: J
authors: Jiang
authors: Changjun
authors: C
authors: Tan
authors: Zude
authors: Z
authors: Xu
authors: Xiangdong
authors: X
authors: Wang
authors: Chunxue
authors: C
authors: Bai
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002199:Capillary Permeability / Q000187:drug effects; D003474:Curcumin / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism*; D003907:Dexamethasone / Q000627:therapeutic use*; D016040:Lung Transplantation / Q000009:adverse effects*; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D016328:NF-kappa B; D003907:Dexamethasone; D003474:Curcumin