[Histopathological techniques for diagnosing cryptococcosis due to capsule-deficient Cryptococcus: case report].
A case of cryptococcosis due to capsule-deficient Cryptococcus is presented, in which culturing of the clinical specimen and tests for capsular antigen in cerebrospinal fluid and serum were negative. The histopathological techniques evaluated were hematoxylin-eosin, Grocott methenamine silver, Mayers mucicarmine and Fontana-Masson. The diagnosis of cryptococcosis due to capsule-deficient Cryptococcus was confirmed by means of the Fontana-Masson technique and by direct immunofluorescence. The potential of the histochemical techniques is discussed.
Gazzoni
Alexandra Flávia
AF
Universidade Federal do Rio Grande do Sul, Porto Alegre, RS.
Pegas
Karla Lais
KL
Severo
Luiz Carlos
LC
D000328:Adult; D003453:Cryptococcosis / Q000473:pathology*; D005260:Female; D006801:Humans; D008172:Lung Diseases, Fungal / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*