title: cis-Jasmone induces Arabidopsis genes that affect the chemical ecology of multitrophic interactions with aphids and their parasitoids.
abstract: It is of adaptive value for a plant to prepare its defenses when a threat is detected, and certain plant volatiles associated with insect damage, such as cis-jasmone (CJ), are known to switch-on defense metabolism. We used aphid and aphid parasitoid responses to Arabidopsis thaliana as a model system for studying gene expression and defense chemistry and its impact at different trophic levels. Differential responses to volatiles of induced Arabidopsis occurred for specialist and generalist insects: the generalist aphid, Myzus persicae, was repelled, whereas the specialist, Lipaphis erysimi, was attracted; the generalist aphid parasitoid Aphidius ervi was attracted, but the specialist parasitoid Diaeretiella rapae was not affected. A. ervi also spent longer foraging on induced plants than on untreated ones. Transcriptomic analyses of CJ-induced Arabidopsis plants revealed that a limited number of genes, including a gene for a cytochrome P450, CYP81D11, were strongly up-regulated in the treated plants. We examined transgenic Arabidopsis lines constitutively overexpressing this gene in bioassays and found insect responses similar to those obtained for wild-type plants induced with CJ, indicating the importance of this gene in the CJ-activated defense response. Genes involved in glucosinolate biosynthesis and catabolism are unaffected by CJ and, because these genes relate to interactions with herbivores and parasitoids specific to this family of plants (Brassicaceae), this finding may explain the differences in behavioral response of specialist and generalist insects.
authors: Rothamsted Research, Harpenden AL5 2JQ, United Kingdom.
authors: Toby J A
authors: TJ
authors: Bruce
authors: Michaela C
authors: MC
authors: Matthes
authors: Keith
authors: K
authors: Chamberlain
authors: Christine M
authors: CM
authors: Woodcock
authors: Abdul
authors: A
authors: Mohib
authors: Ben
authors: B
authors: Webster
authors: Lesley E
authors: LE
authors: Smart
authors: Michael A
authors: MA
authors: Birkett
authors: John A
authors: JA
authors: Pickett
authors: Johnathan A
authors: JA
authors: Napier
mesh_terms: D000818:Animals; D001042:Aphids / Q000187:drug effects / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000469:parasitology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001681:Biological Assay; D015152:Blotting, Northern; D002849:Chromatography, Gas; D003517:Cyclopentanes / Q000494:pharmacology*; D004463:Ecology; D017753:Ecosystem; D005247:Feeding Behavior; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D006790:Host-Parasite Interactions / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology*; D010271:Parasites / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D012333:RNA, Messenger; C086299:jasmone