title: Effects of Cordyceps sinensis, Cordyceps militaris and their isolated compounds on ion transport in Calu-3 human airway epithelial cells.
abstract: OBJECTIVE
  The traditional Chinese medicine Cordyceps sinensis (CS) (Clavicipitaceae) improves pulmonary function and is used to treat respiratory disease. Here, we compare the efficacy and mechanisms of action of Cordyceps sinensis and Cordyceps militaris (CM) (Clavicipitaceae) in Calu-3 human airway epithelial monolayer model.
  
  
  METHODS
  The extracts of Cordyceps sinensis and Cordyceps militaris, as well as their isolated compounds, cordycepin and adenosine, stimulated ion transport in a dose-dependent manner in Calu-3 monolayers. In subsequent experiments, transport inhibitor bumetanide and carbonic anhydrase inhibitor acetazolamide were added after Cordyceps sinensis and Cordyceps militaris extracts to determine their effects on Cl- and HCO3- movement.
  
  
  RESULTS
  The results suggested that Cordyceps sinensis and Cordyceps militaris extracts may affect the anion movement from the basolateral to apical compartments in the airway epithelia.
  
  
  CONCLUSIONS
  Basolateral Na+-K+-2Cl- cotransporter and apical cAMP-dependent cystic fibrosis transmembrane conductance regulator Cl- channel are involved in the process. The results provide the first evidence for the pharmacological mechanism of Cordyceps sinensis and Cordyceps militaris on respiratory tract.
authors: Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China.
authors: Grace Gar-Lee
authors: GG
authors: Yue
authors: Clara Bik-San
authors: CB
authors: Lau
authors: Kwok-Pui
authors: KP
authors: Fung
authors: Ping-Chung
authors: PC
authors: Leung
authors: Wing-Hung
authors: WH
authors: Ko
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D032661:Cordyceps / Q000737:chemistry*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000502:physiology; D003839:Deoxyadenosines / Q000494:pharmacology*; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D017136:Ion Transport / Q000187:drug effects; D028021:Sodium-Potassium-Chloride Symporters / Q000502:physiology
chemical_list: D002712:Chlorides; D003839:Deoxyadenosines; D028021:Sodium-Potassium-Chloride Symporters; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; C058120:cordycepin; D000241:Adenosine