Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells.
The advancement of Drosophila germline stem cell research accompanies the development of powerful new tools for genetic analysis. These include the techniques of stem cell labeling, cell lineage tracing, mosaic mutant analysis, and gene manipulation in targeted cell populations, which together constitute the critical methodologies in stem cell research. We discuss four such techniques: the tubulin-lacZ positive-labeling system; the positively marked mosaic lineage (PMML) method; the flipase/flipase recombination target (FLP/FRT)-based mosaic mutant analysis; and the GAL80-based mosaic analysis with a repressible cell marker (MARCM) system.
Liu
Wei
W
Mouse Cancer Genetics Program, National Institutes of Health, National Cancer Institute at Frederick, Frederick, MD, USA.
Hou
Steven X
SX
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila / Q000166:cytology* / Q000235:genetics*; D005260:Female; D005821:Genetic Techniques; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007763:Lac Operon; D008297:Male; D009030:Mosaicism; D009154:Mutation; D011994:Recombinant Proteins / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D014404:Tubulin / Q000235:genetics
C055002:GAL80 protein, S cerevisiae; D011994:Recombinant Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins