Imaging exocytosis of single insulin secretory granules with TIRF microscopy.
The total internal reflection fluorescence (TIRF) technique is a powerful tool for visualizing the process of insulin exocytosis. This technique allows us to observe the events just beneath the plasma membrane, allowing us to explore the molecular mechanism of insulin exocytotic processes such as docking and fusion. In this review, we describe the method of TIRF for visualizing insulin exocytosis in pancreatic beta-cells.
Nagamatsu
Shinya
S
Department of Biochemistry, Kyorin University School of Medicine, Tokyo, Japan.
Ohara-Imaizumi
Mica
M
D000818:Animals; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D005089:Exocytosis; D049452:Green Fluorescent Proteins; D006801:Humans; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008561:Membrane Fusion; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted; D014162:Transfection
D007328:Insulin; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins