Quantitative agar-invasion assay.
A new method for quantification of yeast invasion into the agar medium was developed. Classical agar-invasion assays have been the methods of choice for determination of yeast invasion, but their main disadvantage is the lack of quantification. Our new Quantitative yeast agar-invasion test allows for quantitative measurements and enables sorting strains by their degree of invasiveness. The invasion abilities were measured for 10 clinical and non-clinical Saccharomyces cerevisiae strains and a strain of Candida albicans. Finally, the correlation between the degrees of strains invasiveness and their reported virulence was observed, proposing our assay as a method for quick determination of yeast virulence potential.
Zupan
Jure
J
Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
Raspor
Peter
P
D000362:Agar; D002176:Candida albicans / Q000254:growth & development / Q000472:pathogenicity; D002177:Candidiasis / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media; D009172:Mycology / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000254:growth & development; D013223:Statistics as Topic; D014774:Virulence; D015003:Yeasts / Q000254:growth & development*
D003470:Culture Media; D000362:Agar