title: Effect of antimicrobial peptides divergicin M35 and nisin A on Listeria monocytogenes LSD530 potassium channels.
abstract: The aim of this work was to study the effect of antimicrobial peptides: divergicin M35 and nisin A on Listeria monocytogenes LSD 530 potassium (K+) channels: ATP-sensitive (K ATP), calcium-activated (BK Ca), and depolarization-activated (Kv) types. Increase on K+ efflux and inhibition of cellular growth were observed after adding K+ channel activators pinacidil, NS1619, and cromakalim to divergicin M35. Increase in K+ efflux from log-phase cells was about 18 +/- 1.1, 11 +/- 0.63, and nmol mg(-1) of cell dry weight (CDW) for pinacidil and NS1619, respectively, over the efflux obtained with divergicin M35 alone. Increases in K+ efflux obtained by adding the same K+ channel activators to nisin A fit a completely different profile. Divergicin M35 activates K+ channels, particularly of the Kv and BK Ca types and to a lesser extent the K ATP type, causing K+ efflux and consequently cell death.
authors: STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute, Université Laval, G1K 7P4, Québec, QC, Canada.
authors: Karim
authors: K
authors: Naghmouchi
authors: Djamel
authors: D
authors: Drider
authors: Riadh
authors: R
authors: Hammami
authors: Ismail
authors: I
authors: Fliss
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology*; D015221:Potassium Channels / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D015221:Potassium Channels; C514464:divergicin M35, Carnobacterium divergicin M35; D009561:Nisin; C102526:nisin A