Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry.
BACKGROUND
The European Centre for the Validation of Alternative Methods (ECVAM) designed the Embryonic Stem Cell Test (EST) as a tool for classifying developmentally toxic compounds. An in vitro tool to assess developmental toxicity would be of great value to the pharmaceutical industry to help with toxicity-associated attrition.


METHODS
ECVAM's EST protocol was used, but employing a different mouse embryonic stem cell (ESC) line and an alternative differentiation medium. A subset of the compounds used to validate the EST assay along with a number of in-house pharmaceutical compounds plus marketed pharmaceutical compounds were used to assess the EST performance with receptor-mediated compounds.


RESULTS
Our results with ECVAM compounds mirrored ECVAM's. Compounds that were developmentally toxic in vivo were classified by the EST as moderate risk. Overall, the accuracy was 75% with the current set of data and the predictivity of low-, moderate-, and high-risk compounds was 90, 71, and 60% while the precision was 59, 86, and 100%, respectively. Interestingly, a number of the non-developmentally toxic compounds had values for the 3T3 IC(50) values, which were lower than the ESC IC(50) and ID(50), a situation not taken into account by ECVAM when designing the EST algorithm.


CONCLUSIONS
The assay as currently constructed has a significant false-positive rate (approximately 40%), but a very low false-negative rate (approximately 7%). Additional moderate- and high-risk compounds need to be assessed to increase confidence, accuracy, and understanding in the EST's predictivity.
Paquette
Jennifer A
JA
Pfizer Global Research & Development, Global DART, Groton/New London Laboratories, Pfizer Inc., Groton, CT 06340, USA. jennifer.a.paquette@pfizer.com
Kumpf
Steven W
SW
Streck
Randal D
RD
Thomson
Jason J
JJ
Chapin
Robert E
RE
Stedman
Donald B
DB
D000826:Animal Testing Alternatives / Q000379:methods; D000818:Animals; D041702:BALB 3T3 Cells; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004345:Drug Industry / Q000379:methods*; D053595:Embryonic Stem Cells / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008811:Mice, Inbred DBA; D018675:Toxicity Tests / Q000379:methods*