title: An ABC transporter encoding gene lndW confers resistance to landomycin E.
abstract: Streptomyces globisporus 1912 produces a polyketide antibiotic landomycin E (LaE), which possesses anticancer activity. A 1.8 kb DNA fragment at the end of landomycin E biosynthetic gene cluster was sequenced. DNA sequence analysis of this fragment identified one complete open reading frame, designated lndW. The deduced sequence of lndW gene product revealed significant similarity to the ATP-binding domains of the ABC (ATP-binding protein cassette) superfamily of transport-related proteins. Knockout of lndW had no significant effect on resistance to LaE and its production. The expression of lndW in S. globisporus 1912 was proven via transcriptional fusion of lndW promoter to EGFP (enhanced green fluorescent protein). Overexpression of lndW in S. lividans TK24 conferred resistance to LaE. The mechanism of lndW function in LaE biosynthesis is discussed.
authors: Department of Genetics and Biotechnology, Ivan Franko National University of L'viv, Grushevskogo st.4, L'viv, 79005, Ukraine.
authors: Iryna
authors: I
authors: Ostash
authors: Yuriy
authors: Y
authors: Rebets
authors: Bohdan
authors: B
authors: Ostash
authors: Anton
authors: A
authors: Kobylyanskyy
authors: Maksym
authors: M
authors: Myronovskyy
authors: Tatsunosuke
authors: T
authors: Nakamura
authors: Suzanne
authors: S
authors: Walker
authors: Victor
authors: V
authors: Fedorenko
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000617:Aminoglycosides / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D015870:Gene Expression; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D010957:Plasmids; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*
chemical_list: D018528:ATP-Binding Cassette Transporters; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; C113593:landomycin E