Wash-free, antibody-assisted magnetoreduction assays of orchid viruses.
This study demonstrates the feasibility of wash-free magnetoreduction assays (MRA) of orchid viruses. A magnetic reagent, consisting of magnetic beads coated with antibodies and dispersed in water, was synthesized. By using a mixed-frequency alternative-current (ac) magnetosusceptometer, differences in the magnetic susceptibilities of the magnetic reagent before and after the addition of orchid solutions were measured. The results show significant advantages for MRA of orchid viruses.
Yang
S Y
SY
Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan.
Jian
Z F
ZF
Chieh
J J
JJ
Horng
H E
HE
Yang
H C
HC
Huang
I J
IJ
Hong
Chin-Yih
CY
D000914:Antibodies, Viral / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D008280:Magnetics; D008863:Microspheres; D029595:Orchidaceae / Q000821:virology*; D010942:Plant Viruses / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*
D000914:Antibodies, Viral