title: High expression levels of cyclin B1 and Polo-like kinase 1 in ectopic endometrial cells associated with abnormal cell cycle regulation of endometriosis.
abstract: OBJECTIVE
  To investigate the possible roles of cyclin B1/cyclin-dependent kinase (cdc2) and Polo-like kinase 1 (Plk1) in the pathogenesis of endometriosis.
  
  
  METHODS
  A case-control study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Patients with or without endometriosis were diagnosed by pathological examination or laparoscopy. The patients with the following criteria within the past 6 months were excluded: endocrine or inflammatory diseases, pregnancy or lactation, hormonal therapy, and neoplasm in the uterine cavity.
  
  
  METHODS
  Eutopic and ectopic endometria were obtained at the time of surgery. Blood was collected on the same day as surgery.
  
  
  METHODS
  The mRNA/protein expression and localization of cyclin B1, cdc2, and Plk1 in endometrium, and serum levels of E(2) and P.
  
  
  RESULTS
  The expression levels of cyclin B1 and Plk1, but not cdc2, in ectopic endometria were significantly higher than in eutopic endometria. The immunohistochemical staining of cyclin B1 and Plk1 was detected in the nuclei of ectopic and eutopic endometrial cells. Furthermore, ectopic endometrial expression levels of cyclin B1 or Plk1 were positively correlated with serum E(2) levels.
  
  
  CONCLUSIONS
  Cyclin B1 and Plk1 may play important roles in the pathogenesis of endometriosis by mediating ectopic endometrial cell proliferation under regulation of ovarian hormones.
authors: Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors: Li
authors: L
authors: Tang
authors: Ting-Ting
authors: TT
authors: Wang
authors: Yan-Ting
authors: YT
authors: Wu
authors: Cai-Yun
authors: CY
authors: Zhou
authors: He-Feng
authors: HF
authors: Huang
mesh_terms: D000328:Adult; D016203:CDC2 Protein Kinase; D002453:Cell Cycle / Q000235:genetics* / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D019926:Cyclin B / Q000235:genetics* / Q000378:metabolism; D056744:Cyclin B1; D018844:Cyclin-Dependent Kinases; D004715:Endometriosis / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D005260:Female; D005498:Follicular Phase / Q000097:blood / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008183:Luteal Phase / Q000097:blood / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D011374:Progesterone / Q000097:blood / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation; D014591:Uterine Diseases / Q000097:blood / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055815:Young Adult; D000097869:Polo-Like Kinase 1
chemical_list: D016203:CDC2 Protein Kinase; D018797:Cell Cycle Proteins; D019926:Cyclin B; D056744:Cyclin B1; D018844:Cyclin-Dependent Kinases; D004958:Estradiol; D011374:Progesterone; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C534699:CCNB1 protein, human; C528899:CDK1 protein, human