Theoretical investigation of the reaction mechanism of the dinuclear zinc enzyme dihydroorotase.
The reaction mechanism of the dinuclear zinc enzyme dihydroorotase was investigated by using hybrid density functional theory. This enzyme catalyzes the reversible interconversion of dihydroorotate and carbamoyl aspartate. Two reaction mechanisms in which the important active site residue Asp250 was either protonated or unprotonated were considered. The calculations establish that Asp250 must be unprotonated for the reaction to take place. The bridging hydroxide is shown to be capable of performing nucleophilic attack on the substrate from its bridging position and the role of Zn(beta) is argued to be the stabilization of the tetrahedral intermediate and the transition state leading to it, thereby lowering the barrier for the nucleophilic attack. It is furthermore concluded that the rate-limiting step is the protonation of the amide nitrogen by Asp250 coupled with C-N bond cleavage, which is consistent with previous experimental findings from isotope labeling studies.
Liao
Rong-Zhen
RZ
Department of Theoretical Chemistry, School of Biotechnology, Royal Institute of Technology, 10691 Stockholm, Sweden.
Yu
Jian-Guo
JG
Raushel
Frank M
FM
Himo
Fahmi
F
D001665:Binding Sites; D018360:Crystallography, X-Ray; D004080:Dihydroorotase / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011487:Protein Conformation; D013816:Thermodynamics; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism*
D004080:Dihydroorotase; D015032:Zinc