[JNK/p38 MAPK involves in ginsenoside Rb1 attenuating beta-amyloid peptide (25-35) -induced tau protein hyperphosphorylation in embryo rat cortical neurons].
To explore the effect of ginsenoside Rb1 on JNK/p38 MAPK in the process of beta-amyloid peptide (25-35) -induced tau protein hyperphosphorylation, Western blotting and immunocytochemical stain were performed to observe the tau protein phosphorylation and the expression of JNK/p38 MAPK. The level of tau protein phosphorylation in the sites of Ser396 , Ser199/202 and Thr205 increased after rat cortical neurons exposed to 20 micromol x L(-1) Abeta25-35, meanwhile the level of JNK/p38 MAPK also increased after Abeta25-35 treatment for 12 h. Pretreatment with several doses of ginsenoside Rbl markedly attenuated tau protein hyperphosphorylation and the expression of JNK/p38 MAPK. Ginsenoside Rbl markedly attenuated tau protein hyperphosphorylation through JNK/p38 MAPK pathway.
Song
Jin-Qiu
JQ
Fujian Institute of Geriatrics, Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
Chen
Xiao-Chun
XC
Zhang
Jing
J
Huang
Tian-Wen
TW
Zeng
Yu-Qi
YQ
Shen
Jie
J
Chen
Li-Min
LM
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D036145:Ginsenosides / Q000302:isolation & purification / Q000494:pharmacology*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D005894:Panax / Q000737:chemistry; D010446:Peptide Fragments / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
D016229:Amyloid beta-Peptides; D036145:Ginsenosides; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35); D016875:tau Proteins; C442759:ginsenoside Rb1; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases