title: Transcriptional activation by GAGA factor is through its direct interaction with dmTAF3.
abstract: The GAGA factor (GAF), encoded by the Trithorax like gene (Trl) is a multifunctional protein involved in gene activation, Polycomb-dependent repression, chromatin remodeling and is a component of chromatin domain boundaries. Although first isolated as transcriptional activator of the Drosophila homeotic gene Ultrabithorax (Ubx), the molecular basis of this GAF activity is unknown. Here we show that dmTAF3 (also known as BIP2 and dTAF(II)155), a component of TFIID, interacts directly with GAF. We generated mutations in dmTAF3 and show that, in Trl mutant background, they affect transcription of Ubx leading to enhancement of Ubx phenotype. These results reveal that the gene activation pathway involving GAF is through its direct interaction with dmTAF3.
authors: Center for Cellular and Molecular Biology, Uppal Road, Hyderabad, 500007, India.
authors: Vivek Sarojkumar
authors: VS
authors: Chopra
authors: Arumugam
authors: A
authors: Srinivasan
authors: Ram Parikshan
authors: RP
authors: Kumar
authors: Krishnaveni
authors: K
authors: Mishra
authors: Denis
authors: D
authors: Basquin
authors: Mylène
authors: M
authors: Docquier
authors: Carole
authors: C
authors: Seum
authors: Daniel
authors: D
authors: Pauli
authors: Rakesh Kumar
authors: RK
authors: Mishra
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D018398:Homeodomain Proteins / Q000378:metabolism; D008957:Models, Genetic; D016296:Mutagenesis; D035381:TATA-Binding Protein Associated Factors / Q000378:metabolism*; D035362:Transcription Factor TFIID / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics / Q000502:physiology*
chemical_list: C432688:BIP2 protein, Drosophila; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D014157:Transcription Factors; C063460:Trl protein, Drosophila; C066177:Ubx protein, Drosophila