title: Regions downstream from the WW domain of dystrophin are important for binding to postsynaptic densities in the brain.
abstract: In order to investigate the mechanism of dystrophin localization in the central nervous system (CNS), we generated adenovirus vectors that contained minidystrophin or truncated minidystrophin cDNA. We infected a primary neuronal culture derived from mdx mouse hippocampus with these viruses. Minidystrophin was observed along the plasma membrane as punctate dots or very short segments. In double immunofluorescence staining with anti-dystrophin and anti-postsynaptic density-95 antibodies, we observed that these proteins entirely colocalized. On the other hand, the truncated minidystrophin, which has deleted WW, cysteine-rich and C-terminal domains, was homogenously expressed in cytoplasm, neurites and axons. These findings suggest that a binding site to postsynaptic densities exists in the region extending from the WW domain to the C-terminal domain of dystrophin and that this site is necessary for binding to membrane.
authors: Department of Neurology, Kumamoto University Graduate School of Medical Sciences, 1-1-1 Honjo, Kumamoto, Kumamoto 860-0811, Japan. sakatetsu@fc.kuh.kumamoto-u.ac.jp <sakatetsu@fc.kuh.kumamoto-u.ac.jp>
authors: Tetsuro
authors: T
authors: Sakamoto
authors: Toshiyuki
authors: T
authors: Arima
authors: Masatoshi
authors: M
authors: Ishizaki
authors: Ryoko
authors: R
authors: Kawano
authors: Tatsuya
authors: T
authors: Koide
authors: Yuji
authors: Y
authors: Uchida
authors: Satoshi
authors: S
authors: Yamashita
authors: En
authors: E
authors: Kimura
authors: Teruyuki
authors: T
authors: Hirano
authors: Yasushi
authors: Y
authors: Maeda
authors: Makoto
authors: M
authors: Uchino
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D019556:COS Cells; D018929:Cell Culture Techniques; D002460:Cell Line; D002522:Chlorocebus aethiops; D016189:Dystrophin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D008969:Molecular Sequence Data; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D013569:Synapses / Q000378:metabolism*; D014162:Transfection
chemical_list: D016189:Dystrophin; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins