Protein extraction from green plant tissue.
A method for preparation of protein from green plant tissue for two-dimensional electrophoresis is described. The method is demonstrated on barley leaves, potato leaves and spruce needles and appears to overcome the obstacles inherent in green plants to proteomic analysis. The yield and the representation of proteins are discussed.
Flengsrud
Ragnar
R
Norwegian University of Life Sciences, As, Norway.
D015180:Electrophoresis, Gel, Two-Dimensional; D010940:Plant Proteins / Q000302:isolation & purification*; D010944:Plants / Q000737:chemistry*
D010940:Plant Proteins