Principles of Dicer substrate (D-siRNA) design and function.
An efficient RNAi largely depends on optimal design of the siRNA. In recent studies, Dicer substrates were found to be more potent than classical synthetic 21-mer siRNAs, suggesting a coupling of the Dicer-mediated processing step to the efficient assembly of the silencing complex, RISC. We describe the fundamental principles and experimental results leading to optimal Dicer substrates.
Amarzguioui
Mohammed
M
The Biotechnology Centre of Oslo, Oslo, Norway.
Rossi
John J
JJ
D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism
D034741:RNA, Small Interfering; D043244:Ribonuclease III