Astrin regulates Aurora-A localization.
Alterations in the expression and activity of the centrosomal kinase, Aurora-A/STK15, affect genomic stability, disrupt the fidelity of centrosome duplication, and induce cellular transformation. A mitotic spindle-associated protein, astrin/DEEPEST, was identified as an Aurora-A interacting protein by a two-hybrid screen. Astrin and Aurora-A co-express at mitosis and co-localize to mitotic spindles. RNAi-mediated depletion of astrin abolishes the localization of Aurora-A on mitotic spindles and leads to a moderate mitotic cell cycle delay, which resembles the mitotic arrest phenotypes in siAurora-A treated cells. However, depletion of Aurora-A does not affect astrin localization, and co-depletion of both astrin and Aurora-A causes a mitotic arrest phenotype similar to depletion of siAurora-A alone. These results suggest that astrin acts upstream of Aurora-A to regulate its mitotic spindle localization.
Du
Jian
J
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Jablonski
Sandra
S
Yen
Tim J
TJ
Hannon
Gregory J
GJ
D064096:Aurora Kinase A; D064127:Aurora Kinases; D018797:Cell Cycle Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018385:Centrosome / Q000201:enzymology; D004843:Epistasis, Genetic; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis / Q000187:drug effects / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D012441:Saccharomyces cerevisiae / Q000235:genetics; D008941:Spindle Apparatus / Q000201:enzymology*; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; C486663:SPAG5 protein, human; C576917:AURKA protein, human; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases