Diversity of errantivirus (retrovirus) sequences in two cell lines used for baculovirus expression, Spodoptera frugiperda and Trichoplusia ni.
To determine the diversity of retroviruses (errantiviruses) in cell lines used for baculovirus expression, degenerate primers were designed complementary to conserved regions of lepidopteran errantivirus reverse transcriptase genes. These primers were used to PCR amplify sequences from DNA isolated from Spodoptera frugiperda (Sf-9) and Trichoplusia ni (Hi-5) cell lines. Cloning, sequencing, and phylogenetic analysis of over 20 PCR products from each cell line demonstrated the presence of diverse populations of retrovirus sequences comprising at least six major lineages.
Menzel
Tila
T
Department of Microbiology, Oregon State University, Nash Hall Room 220, Corvallis, OR 97331-3804, USA.
Rohrmann
George F
GF
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics; D012190:Retroviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase