title: Adiponectin induces insulin secretion in vitro and in vivo at a low glucose concentration.
abstract: OBJECTIVE
  A decrease in plasma adiponectin levels has been shown to contribute to the development of diabetes. However, it remains uncertain whether adiponectin plays a role in the regulation of insulin secretion. In this study, we investigated whether adiponectin may be involved in the regulation of insulin secretion in vivo and in vitro.
  
  
  METHODS
  The effect of adiponectin on insulin secretion was measured in vitro and in vivo, along with the effects of adiponectin on ATP generation, membrane potentials, Ca2+ currents, cytosolic calcium concentration and state of 5'-AMP-activated protein kinase (AMPK). In addition, insulin granule transport was measured by membrane capacitance and total internal reflection fluorescence (TIRF) analysis.
  
  
  RESULTS
  Adiponectin significantly stimulated insulin secretion from pancreatic islets to approximately 2.3-fold the baseline value in the presence of a glucose concentration of 5.6 mmol/l. Although adiponectin had no effect on ATP generation, membrane potentials, Ca2+ currents, cytosolic calcium concentrations or activation status of AMPK, it caused a significant increase of membrane capacitance to approximately 2.3-fold the baseline value. TIRF analysis revealed that adiponectin induced a significant increase in the number of fusion events in mouse pancreatic beta cells under 5.6 mmol/l glucose loading, without affecting the status of previously docked granules. Moreover, intravenous injection of adiponectin significantly increased insulin secretion to approximately 1.6-fold of baseline in C57BL/6 mice.
  
  
  CONCLUSIONS
  The above results indicate that adiponectin induces insulin secretion in vitro and in vivo.
authors: Department of Metabolic Diseases, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Tokyo, 113-8655, Japan.
authors: M
authors: M
authors: Okamoto
authors: M
authors: M
authors: Ohara-Imaizumi
authors: N
authors: N
authors: Kubota
authors: S
authors: S
authors: Hashimoto
authors: K
authors: K
authors: Eto
authors: T
authors: T
authors: Kanno
authors: T
authors: T
authors: Kubota
authors: M
authors: M
authors: Wakui
authors: R
authors: R
authors: Nagai
authors: M
authors: M
authors: Noda
authors: S
authors: S
authors: Nagamatsu
authors: T
authors: T
authors: Kadowaki
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004594:Electrophysiology; D005947:Glucose / Q000494:pharmacology*; D006019:Glycolysis; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D019308:Palmitic Acid / Q000378:metabolism
chemical_list: D052242:Adiponectin; D007328:Insulin; D019308:Palmitic Acid; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D005947:Glucose