Biosensing approach for glutathione detection using glutathione reductase and sulfhydryl oxidase bienzymatic system.
Chitosan membrane with glutathione reductase and sulfhydryl oxidase (SOX) was subsequently integrated onto the surface of spectrographic graphite rods for obtaining a glutathione biosensor. The working principle was based on the monitoring of O(2) consumption that correlates the concentration of glutathione during the enzymatic reaction. A linear relationship between sensor response and concentration was obtained between 0.5 and 2.0 mM for oxidized glutathione (GSSG), and 0.2-1.0 mM for reduced glutathione (GSH) in the presence of 2 microM nicotinamide adenine dinucleotide phosphate (NADPH) under the optimum working conditions. Also, reduced/oxidized glutathione were separated by HPLC and utility of bienzymatic system was investigated as an electrochemical detector for the analysis of these compounds. All data were given as a comparison of two systems: biosensor and diode array detector (DAD).
Timur
Suna
S
Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkiye.
Odaci
Dilek
D
Dincer
Ayse
A
Zihnioglu
Figen
F
Telefoncu
Azmi
A
D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized; D005978:Glutathione / Q000032:analysis*; D019803:Glutathione Disulfide; D005980:Glutathione Reductase / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors
D004800:Enzymes, Immobilized; D005978:Glutathione; D019803:Glutathione Disulfide; D005980:Glutathione Reductase; D010088:Oxidoreductases; D010100:Oxygen; D050862:Oxidoreductases Acting on Sulfur Group Donors