title: Comparison of cellular ribonucleoprotein complexes associated with the APOBEC3F and APOBEC3G antiviral proteins.
abstract: The human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F (APOBEC3F [A3F]) and A3G proteins are effective inhibitors of infection by various retroelements and share approximately 50% amino acid sequence identity. We therefore undertook comparative analyses of the protein and RNA compositions of A3F- and A3G-associated ribonucleoprotein complexes (RNPs). Like A3G, A3F is found associated with a complex array of cytoplasmic RNPs and can accumulate in RNA-rich cytoplasmic microdomains known as mRNA processing bodies or stress granules. While A3F RNPs display greater resistance to disruption by RNase digestion, the major protein difference is the absence of the Ro60 and La autoantigens. Consistent with this, A3F RNPs also lack a number of small polymerase III RNAs, including the RoRNP-associated Y RNAs, as well as 7SL RNA. Alu RNA is, however, present in A3F and A3G RNPs, and both proteins suppress Alu element retrotransposition. Thus, we define a number of subtle differences between the RNPs associated with A3F and A3G and speculate that these contribute to functional differences that have been described for these proteins.
authors: Department of Infectious Diseases, King's College London School of Medicine, London SE1 9RT, United Kingdom.
authors: Sarah
authors: S
authors: Gallois-Montbrun
authors: Rebecca K
authors: RK
authors: Holmes
authors: Chad M
authors: CM
authors: Swanson
authors: Mireia
authors: M
authors: Fernández-Ocaña
authors: Helen L
authors: HL
authors: Byers
authors: Malcolm A
authors: MA
authors: Ward
authors: Michael H
authors: MH
authors: Malim
mesh_terms: D000071480:APOBEC-3G Deaminase; D002460:Cell Line; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D043525:Cytosine Deaminase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D012261:Ribonucleoproteins / Q000378:metabolism*
chemical_list: D012261:Ribonucleoproteins; C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase