Pregnancy after trophectoderm biopsy of frozen-thawed blastocyst.
OBJECTIVE
To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst.


METHODS
Techniques and instrumentation.


METHODS
A University Medical Center.


METHODS
Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program.


METHODS
Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance.


METHODS
Serum betahCG and transvaginal ultrasound.


RESULTS
Positive betahCG and ongoing pregnancy.


CONCLUSIONS
Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.
Lathi
Ruth Bunker
RB
Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Stanford University Medical Center, Stanford, California 94305, USA. rlathi@stanford.edu
Behr
Barry
B
D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000502:physiology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood; D002970:Cleavage Stage, Ovum / Q000473:pathology; D004624:Embryo Transfer; D005260:Female; D005615:Freezing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D014327:Trophoblasts / Q000166:cytology
D018997:Chorionic Gonadotropin, beta Subunit, Human