The complete nucleotide sequence of the type 5 Helicoverpa armigera cytoplasmic polyhedrosis virus genome.
The S1-6, S8, and S9 segments of the type 5 Helicoverpa armigera cytoplasmic polyhedrosis virus (HaCPV-5, Chinese strain) were cloned and sequenced, completing the HaCPV-5 genome. We found that each HaCPV-5 segment exhibits the conserved terminal sequences AGUU and UUGC located at the 5' and 3' ends, respectively. We also analyzed the translation initiation codon of the HaCPV-5 genome and compared it with the available cypovirus sequences in GenBank. We postulated that the conserved purine at the -3 position in relation to the AUG codon is probably the most important nucleotide for efficient translation initiation in cypovirus. Although the nucleotide sequences of the HaCPV-5 segments S1-10 exhibit no significant similarity to other viruses, blast searches did reveal some similarities between predicted HaCPV-5 amino acid sequences and those of other viruses.
Tan
Li
L
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, 430072, China.
Zhang
Jiamin
J
Li
Yang
Y
Li
Yanqiu
Y
Jiang
Hong
H
Cao
Xu
X
Hu
Yuanyang
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics; D012087:Reoviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase