Simultaneous analysis of five antidepressant drugs using direct injection of biofluids in a capillary restricted-access media-liquid chromatography-tandem mass spectrometry system.
Direct analysis, with minimal sample pretreatment, of antidepressant drugs, fluoxetine, imipramine, desipramine, amitriptyline, and nortriptyline in biofluids was developed with a total run time of 8 min. The setup consists of two HPLC pumps, injection valve, capillary RAM-ADS-C18 pre-column and a capillary analytical C18 column connected by means of a six-port valve in backflush mode. Detection was performed with ESI-MS/MS and only 1 microm of sample was injected. Validation was adequately carried out using FLU-d(5) as internal standard. Calibration curves were constructed under a linear range of 1-250 ng mL(-1) in plasma, being the limit of quantification (LOQ), determined as 1 ng mL(-1), for all the analytes. With the described approach it was possible to reach a quantified mass sensitivity of 0.3 pg for each analyte (equivalent to 1.1-1.3 fmol), translating to a lower sample consumption (in the order of 10(3) less sample than using conventional methods).
Santos-Neto
Alvaro J
AJ
Laboratory of Chromatography, Institute of Chemistry at Sao Carlos, University of Sao Paulo, P.O. Box 780, Av. Trabalhador Saocarlense 400, 13566-590 Sao Carlos, Brazil.
Bergquist
Jonas
J
Lanças
Fernando M
FM
Sjöberg
Per J R
PJ
D000928:Antidepressive Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007540:Isonipecotic Acids / Q000032:analysis / Q000737:chemistry; D008614:Meperidine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000928:Antidepressive Agents; D007540:Isonipecotic Acids; D010636:Phenols; C011044:norketobemidone; D008614:Meperidine; C012394:ketobemidone