New validated liquid chromatographic and chemometrics-assisted UV spectroscopic methods for the determination of two multicomponent cough mixtures in syrup.
Multivariate spectrophotometric calibration and liquid chromatographic (LC) methods were applied to the determination of 2 multicomponent mixtures containing diprophylline, guaiphenesin, methylparaben, and propylparaben (Mixture 1), or clobutinol, orciprenaline, saccharin sodium, and sodium benzoate (Mixture 2). For the multivariate spectrophotometric calibration methods, principal component regression (PCR) and partial least-squares regression (PLS-1), a calibration set of the mixtures consisting of the components of each mixture was prepared in 0.1 M HCl. Analytical figures of merit such as sensitivity, selectivity, limit of quantitation, and limit of detection were determined for both PLS-1 and PCR. The LC separation was achieved on a reversed-phase C18 analytical column by using isocratic elution with 20 mM potassium dihydrogen phosphate, pH 3.3-acetonitrile (55 + 45, v/v) as the mobile phase and UV detection at 260 and 220 nm for Mixture 1 and Mixture 2, respectively. The proposed methods were validated and successfully applied to the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing the 2 multicomponent combinations.
Hadad
Ghada M
GM
Suez Canal University, Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Ismailia 41522, Egypt. ghhadad@yahoo.com
El-Gindy
Alaa
A
Mahmoud
Waleed M M
WM
D000996:Antitussive Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D004400:Dyphylline / Q000032:analysis; D006140:Guaifenesin / Q000032:analysis; D010226:Parabens / Q000032:analysis; D020160:Sodium Benzoate / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000996:Antitussive Agents; D010226:Parabens; D004400:Dyphylline; D006140:Guaifenesin; C015358:methylparaben; D020160:Sodium Benzoate; C006068:propylparaben