title: Akt1/protein kinase B alpha is involved in gastric cancer progression and cell proliferation.
abstract: Akt (also known as protein kinase B, PKB) is involved in a variety of biological processes, for example cell development, proliferation, and angiogenesis. Clinical studies in support of the idea that increased activity of Akt could contribute directly to gastric carcinogenesis are rare, however. In this study we discovered that phospho-Akt1 was overexpressed in human gastric cancers and its levels correlated with tumor differentiation and pTNM. Akt1 activation promoted cell survival, because the phosphatidylinositol 3-kinase(PI3K) inhibitor LY294002 inhibited Akt1 phosphorylation and inhibited cell growth, especially in cells with active Akt1. Dominant negative Akt inhibited proliferation of gastric cancer cells and induced G1 cell-cycle arrest whereas constitutively active Akt increased cell proliferation. We have therefore identified Akt1 as an active kinase that contributes to gastric cancer progression and promotes proliferation of gastric cancer cells.
authors: The State Key Laboratory for Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, PR China.
authors: Zheyi
authors: Z
authors: Han
authors: Kaichun
authors: K
authors: Wu
authors: Huiqin
authors: H
authors: Shen
authors: Chunying
authors: C
authors: Li
authors: Shuang
authors: S
authors: Han
authors: Liu
authors: L
authors: Hong
authors: Yongquan
authors: Y
authors: Shi
authors: Na
authors: N
authors: Liu
authors: Changcun
authors: C
authors: Guo
authors: Yan
authors: Y
authors: Xue
authors: Taidong
authors: T
authors: Qiao
authors: Daiming
authors: D
authors: Fan
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D018450:Disease Progression; D006801:Humans; D007124:Immunoenzyme Techniques; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D013274:Stomach Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
chemical_list: D051057:Proto-Oncogene Proteins c-akt