title: PILRalpha is a herpes simplex virus-1 entry coreceptor that associates with glycoprotein B.
abstract: Glycoprotein B (gB) is one of the essential components for infection by herpes simplex virus-1 (HSV-1). Although several cellular receptors that associate with glycoprotein D (gD), such as herpes virus entry mediator (HVEM) and Nectin-1, have been identified, specific molecules that mediate HSV-1 infection by associating with gB have not been elucidated. Here, we found that paired immunoglobulin-like type 2 receptor (PILR) alpha associates with gB, and cells transduced with PILRalpha become susceptible to HSV-1 infection. Furthermore, HSV-1 infection of human primary cells expressing both HVEM and PILRalpha was blocked by either anti-PILRalpha or anti-HVEM antibody. Our results demonstrate that cellular receptors for both gB and gD are required for HSV-1 infection and that PILRalpha plays an important role in HSV-1 infection as a coreceptor that associates with gB. These findings uncover a crucial aspect of the mechanism underlying HSV-1 infection.
authors: Department of Immunochemistry, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Takeshi
authors: T
authors: Satoh
authors: Jun
authors: J
authors: Arii
authors: Tadahiro
authors: T
authors: Suenaga
authors: Jing
authors: J
authors: Wang
authors: Amane
authors: A
authors: Kogure
authors: Junji
authors: J
authors: Uehori
authors: Noriko
authors: N
authors: Arase
authors: Ikuo
authors: I
authors: Shiratori
authors: Shinya
authors: S
authors: Tanaka
authors: Yasushi
authors: Y
authors: Kawaguchi
authors: Patricia G
authors: PG
authors: Spear
authors: Lewis L
authors: LL
authors: Lanier
authors: Hisashi
authors: H
authors: Arase
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D006561:Herpes Simplex / Q000378:metabolism* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; C412263:PILRA protein, human; D011971:Receptors, Immunologic; D011991:Receptors, Virus; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus