title: Saccharomyces cerevisiae phospholipase C regulates transcription of Msn2p-dependent stress-responsive genes.
abstract: Phosphatidylinositol phosphates are involved in signal transduction, cytoskeletal organization, and membrane trafficking. Inositol polyphosphates, produced from phosphatidylinositol phosphates by the phospholipase C-dependent pathway, regulate chromatin remodeling. We used genome-wide expression analysis to further investigate the roles of Plc1p (phosphoinositide-specific phospholipase C in Saccharomyces cerevisiae) and inositol polyphosphates in transcriptional regulation. Plc1p contributes to the regulation of approximately 2% of yeast genes in cells grown in rich medium. Most of these genes are induced by nutrient limitation and other environmental stresses and are derepressed in plc1 Delta cells. Surprisingly, genes regulated by Plc1p do not correlate with gene sets regulated by Swi/Snf or RSC chromatin remodeling complexes but show correlation with genes controlled by Msn2p. Our results suggest that the increased expression of stress-responsive genes in plc1 Delta cells is mediated by decreased cyclic AMP synthesis and protein kinase A (PKA)-mediated phosphorylation of Msn2p and increased binding of Msn2p to stress-responsive promoters. Accordingly, plc1 Delta cells display other phenotypes characteristic of cells with decreased PKA activity. Our results are consistent with a model in which Plc1p acts together with the membrane receptor Gpr1p and associated G(alpha) protein Gpa2p in a pathway separate from Ras1p/Ras2p and converging on PKA.
authors: Department of Biological Sciences, St. John's University, 8000 Utopia Parkway, Queens, NY 11439, USA.
authors: Agnieszka
authors: A
authors: Demczuk
authors: Nilanjan
authors: N
authors: Guha
authors: Peter H
authors: PH
authors: Nguyen
authors: Parima
authors: P
authors: Desai
authors: Jennifer
authors: J
authors: Chang
authors: Katarzyna
authors: K
authors: Guzinska
authors: Janet
authors: J
authors: Rollins
authors: Chandra C
authors: CC
authors: Ghosh
authors: Leslie
authors: L
authors: Goodwin
authors: Ales
authors: A
authors: Vancura
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D046912:Multiprotein Complexes / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D010738:Type C Phospholipases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; C081935:MSN2 protein, S cerevisiae; D046912:Multiprotein Complexes; D018129:Phosphatidylinositol Phosphates; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D017868:Cyclic AMP-Dependent Protein Kinases; D010738:Type C Phospholipases; C522285:Plc1 protein, S cerevisiae