Comparison of in-house-developed ELISA with HPLC techniques for the analysis of atrazine residues.
In-house developed ELISA was standardized to monitor atrazine residues in different environmental samples. The standard curve was linear, indicating an increase in log concentration with decrease in absorbance (%B/B(0)=1.075-0.042 Log C; r= -0.966). The middle of the test was at 75 ng/L and the lowest detection limit at 4 ng/L. ELISA significantly correlated with the high performance liquid chromatography (HPLC) (r=0.990). Internal validation showed good accuracy and precision. Maximum atrazine residues were present in Jehlum River water/sediments and maize/sugarcane plant roots. Most of the food samples were found to be contaminated. ELISA required less clean-up steps than HPLC, but showed matrix effect in soil/colored extracts.
Maqbool
Uzma
U
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan. niabmail@niab.org.pk
Ul-Haq
Anwar
A
Qureshi
M Jamil
MJ
D001280:Atrazine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004784:Environmental Monitoring; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis; D006540:Herbicides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012621:Seasons; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis
D006540:Herbicides; D010573:Pesticide Residues; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D001280:Atrazine