Comparison of the essential cellular functions of the two murA genes of Bacillus anthracis.
Targeted antisense and gene replacement mutagenesis experiments demonstrate that only the murA1 gene and not the murA2 gene is required for the normal cellular growth of Bacillus anthracis. Antisense-based modulation of murA1 gene expression hypersensitizes cells to the MurA-specific antibiotic fosfomycin despite the normally high resistance of B. anthracis to this drug.
Kedar
G C
GC
Trius Therapeutics, Inc., 6310 Nancy Ridge Drive, Suite 105, San Diego, CA 92121, USA. kgc@triusrx.com
Brown-Driver
Vickie
V
Reyes
Daniel R
DR
Hilgers
Mark T
MT
Stidham
Mark A
MA
Shaw
Karen Joy
KJ
Finn
John
J
Haselbeck
Robert J
RJ
D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001408:Bacillus anthracis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial; D005578:Fosfomycin / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D016372:RNA, Antisense / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016372:RNA, Antisense; D005578:Fosfomycin; D019883:Alkyl and Aryl Transferases; C022566:UDP-N-acetylglucosamine 1-carboxyvinyltransferase