A novel function of dcf1 during the differentiation of neural stem cells in vitro.
The study of neural dendrite formation is of great significance both in theory and applications. However, the molecular mechanisms of regulation remain unclear. We previously described a novel EST, which has high homology with dentritic cell factors (DCF1), expressed differentially between undifferentiated and differentiated neural stem cells (NSCs). In this study, we cloned, expressed, and silenced the dcf1 gene and offered insight into its function in regulating dendrite formation during the differentiation of NSCs. The results indicated that dcf1 encoded a 42 kD protein and could be successfully expressed both in Escherichia coli and NSCs. In order to silence dcf1 gene, three different kinds of siRNA vectors were constructed and transformed into the NSC line C17.2 and primary NSCs, resulting in down regulation of the dcf1 mRNA. Analysis of immunofluorescence or GFP illuminated that with overexpression of the dcf1 gene, the NSCs were maintained in undifferentiated status. After the dcf1 gene was silenced, cells tended to differentiate into neurons and astrocytes.
Wang
Lei
L
The Laboratory of neural molecular biology, School of Life Sciences, Shanghai University, 99 Shangda Road, Shanghai, 200444, China.
Wang
Jiao
J
Wu
Yiliu
Y
Wu
Jie
J
Pang
Shuya
S
Pan
Rong
R
Wen
Tieqiao
T
D000818:Animals; D001253:Astrocytes / Q000502:physiology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003712:Dendrites / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D020868:Gene Silencing; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*
D008565:Membrane Proteins; D034741:RNA, Small Interfering