title: A novel C-type lectin from abalone, Haliotis discus discus, agglutinates Vibrio alginolyticus.
abstract: Owing to its specific binding to carbohydrates, lectins play important roles in pathogen recognition and clearance in invertebrate animals. In this study, a novel C-type lectin (designated CLHd) gene was isolated from abalone, Haliotis discus discus, cDNA library. The complete cDNA sequence of the CLHd gene is 508 base pairs in length, and encodes 151 amino acids. CLHd shares a highly conserved carbohydrate recognition domain with C-type lectins from mollusk and fish. The mRNA expressions of CLHd in healthy and bacterial-challenged abalones were examined using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). CLHd mRNA transcription was up-regulated by Vibrio alginolyticus challenge and reached the maximum expression at 24h after the bacterial injection. To understand its biological activity, the recombinant CLHd gene was constructed and expressed in Escherichia coli. The recombinant CLHd specifically agglutinated V. alginolyticus at a concentration of 50microg/ml in a calcium-dependant way. Both the gene expression analysis and recombinant protein activity assay suggest that CLHd is an important immune gene involved in the recognition and elimination of pathogens in abalones.
authors: Department of Aquatic Life Medicine, Cheju National University, 66 Jejudaehakno, Ara-Dong 690-756, Republic of Korea.
authors: Ning
authors: N
authors: Wang
authors: Ilson
authors: I
authors: Whang
authors: Jehee
authors: J
authors: Lee
mesh_terms: D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002241:Carbohydrates / Q000737:chemistry; D049851:Gastropoda / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D037181:Lectins, C-Type / Q000737:chemistry* / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D044149:Vibrio alginolyticus / Q000276:immunology* / Q000502:physiology
chemical_list: D002241:Carbohydrates; D037181:Lectins, C-Type; D012333:RNA, Messenger; D011994:Recombinant Proteins