title: The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions.
abstract: Saliva is a body fluid with important functions in oral and general health. A consortium of three research groups catalogued the proteins in human saliva collected as the ductal secretions: 1166 identifications--914 in parotid and 917 in submandibular/sublingual saliva--were made. The results showed that a high proportion of proteins that are found in plasma and/or tears are also present in saliva along with unique components. The proteins identified are involved in numerous molecular processes ranging from structural functions to enzymatic/catalytic activities. As expected, the majority mapped to the extracellular and secretory compartments. An immunoblot approach was used to validate the presence in saliva of a subset of the proteins identified by mass spectrometric approaches. These experiments focused on novel constituents and proteins for which the peptide evidence was relatively weak. Ultimately, information derived from the work reported here and related published studies can be used to translate blood-based clinical laboratory tests into a format that utilizes saliva. Additionally, a catalogue of the salivary proteome of healthy individuals allows future analyses of salivary samples from individuals with oral and systemic diseases, with the goal of identifying biomarkers with diagnostic and/or prognostic value for these conditions; another possibility is the discovery of therapeutic targets.
authors: School of Dentistry, University of Southern California, Los Angeles, California 90089, USA.
authors: Paul
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authors: Denny
authors: Fred K
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authors: Hagen
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authors: Hardt
authors: Lujian
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authors: Liao
authors: Weihong
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authors: Yan
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authors: Arellanno
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authors: Bassilian
authors: Gurrinder S
authors: GS
authors: Bedi
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authors: Boontheung
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authors: Cociorva
authors: Claire M
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authors: KF
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authors: Gonzalez-Begne
authors: Frédéric
authors: F
authors: Halgand
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authors: Xuemei
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authors: Bradley
authors: B
authors: Henson
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authors: Hewel
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authors: Hu
authors: Sherry
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authors: Jeffrey
authors: Jiang
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authors: Jiang
authors: Joseph A
authors: JA
authors: Loo
authors: Rachel R
authors: RR
authors: Ogorzalek Loo
authors: Daniel
authors: D
authors: Malamud
authors: James E
authors: JE
authors: Melvin
authors: Olga
authors: O
authors: Miroshnychenko
authors: Mahvash
authors: M
authors: Navazesh
authors: Richard
authors: R
authors: Niles
authors: Sung Kyu
authors: SK
authors: Park
authors: Akraporn
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authors: Prakobphol
authors: Prasanna
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authors: Ramachandran
authors: Megan
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authors: Richert
authors: Sarah
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authors: Robinson
authors: Melissa
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authors: Sondej
authors: Puneet
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authors: Souda
authors: Mark A
authors: MA
authors: Sullivan
authors: Jona
authors: J
authors: Takashima
authors: Shawn
authors: S
authors: Than
authors: Jianghua
authors: J
authors: Wang
authors: Julian P
authors: JP
authors: Whitelegge
authors: H Ewa
authors: HE
authors: Witkowska
authors: Lawrence
authors: L
authors: Wolinsky
authors: Yongming
authors: Y
authors: Xie
authors: Tao
authors: T
authors: Xu
authors: Weixia
authors: W
authors: Yu
authors: Jimmy
authors: J
authors: Ytterberg
authors: David T
authors: DT
authors: Wong
authors: John R
authors: JR
authors: Yates
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authors: Fisher
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000737:chemistry*; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013361:Sublingual Gland / Q000737:chemistry*; D013363:Submandibular Gland / Q000737:chemistry*; D013666:Tears / Q000737:chemistry
chemical_list: D001798:Blood Proteins; D020543:Proteome; D012471:Salivary Proteins and Peptides