Urinary uromodulin carries an intact ZP domain generated by a conserved C-terminal proteolytic cleavage.
Uromodulin (or Tamm-Horsfall protein) is the most abundant protein in human urine under physiological conditions. Little is known about the molecular mechanism of uromodulin secretion. By extensive Mass Spectrometry analyses we mapped the C-termini of human and murine urinary proteins demonstrating that urinary uromodulin is generated by a conserved C-terminal proteolytic cleavage and retains its entire ZP domain.
Santambrogio
Sara
S
Dulbecco Telethon Institute, Molecular Genetics of Renal Disorders, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy.
Cattaneo
Angela
A
Bernascone
Ilenia
I
Schwend
Thomas
T
Jovine
Luca
L
Bachi
Angela
A
Rampoldi
Luca
L
D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000737:chemistry* / Q000378:metabolism / Q000652:urine*; D017434:Protein Structure, Tertiary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D058949:Uromodulin
D009088:Mucoproteins; C548612:UMOD protein, human; C548613:Umod protein, mouse; D058949:Uromodulin