Identification of Marteilia refringens infecting the razor clam Solen marginatus by PCR and in situ hybridization.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. It is believed to have a complex life-cycle involving several hosts. In this study, we applied molecular approaches to identify this parasite in samples of the razor clam Solen marginatus from the south west coast of Spain. We used a PCR assay to amplify a fragment of the IGS rDNA region. PCR products were sequenced and the phylogenetic affinity of the sequences was determined. In situ hybridization analysis showed tissue distribution and presence of different developmental stages of the parasite in the digestive diverticula epithelium, which suggested a true parasitism in these individuals. This is the first report of the occurrence of M. refringens in the razor clam S. marginatus in the south Atlantic. The methodology described herein may be useful for accurate identification of the parasite strain in different hosts and thus provide valuable information for marteiliosis control programmes.
López-Flores
Inmaculada
I
IFAPA Centro Agua del Pino, Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Carretera Cartaya-Punta Umbría, 21459 Huelva, Spain. ilopez@ugr.es
Garrido-Ramos
Manuel A
MA
de la Herran
Roberto
R
Ruiz-Rejón
Carmelo
C
Ruiz-Rejón
Manuel
M
Navas
José I
JI
D000818:Animals; D049872:Bivalvia / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis; D056890:Eukaryota / Q000235:genetics / Q000302:isolation & purification*; D017403:In Situ Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D016054:DNA, Protozoan