title: In vivo tracking of human mesenchymal stem cells in experimental stroke.
abstract: To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model of middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed, hMSCs were labeled with ferumoxides (Feridex)--protamine sulfate complexes, which were visualized and examined by MRI up to 10 weeks following transplantation. Migration of the transplanted cells to the infarcted area was further confirmed by histological methods. We found that the hMSCs transplanted in MCAo models possess the capacity to migrate to the infarcted area extensively in both ipsilateral and contralateral injections, exhibiting a pathotropism. We also analyzed the detailed migration patterns of transplanted hMSCs. We speculate that the extensive migratory ability of hMSCs may represent a therapeutic potential for developing efficient cell transplantation strategies in stroke.
authors: MRI Team, Korea Basic Science Institute, Ochang, Korea.
authors: Daehong
authors: D
authors: Kim
authors: Byoung-Gi
authors: BG
authors: Chun
authors: Yeon-Kyung
authors: YK
authors: Kim
authors: Yong Hyun
authors: YH
authors: Lee
authors: Cheong-Soo
authors: CS
authors: Park
authors: Iksoo
authors: I
authors: Jeon
authors: Chaejoon
authors: C
authors: Cheong
authors: Tae-Sun
authors: TS
authors: Hwang
authors: Hyungmin
authors: H
authors: Chung
authors: Byoung Joo
authors: BJ
authors: Gwag
authors: Kwan Soo
authors: KS
authors: Hong
authors: Jihwan
authors: J
authors: Song
mesh_terms: D000818:Animals; D002465:Cell Movement; D003287:Contrast Media; D003911:Dextrans; D052203:Ferrosoferric Oxide; D006801:Humans; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis / Q000473:pathology / Q000628:therapy*; D007501:Iron; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010087:Oxides; D011479:Protamines; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D011479:Protamines; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide