Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes.
Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.
Celli
Susanna
S
Département d'Immunologie, G5 Dynamiques des Réponses Immunes, Equipe Avenir, Institut Pasteur, Paris Cedex, France.
Breart
Béatrice
B
Bousso
Philippe
P
D019264:Adoptive Transfer; D000758:Anesthesia; D000818:Animals; D002404:Catheterization; D003713:Dendritic Cells / Q000166:cytology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007601:Jugular Veins; D007694:Killer Cells, Natural / Q000166:cytology*; D008198:Lymph Nodes / Q000166:cytology*; D051379:Mice; D018613:Microscopy, Confocal; D017785:Photons; D013696:Temperature