Point mutations abolishing the mannose-binding capability of boar spermadhesin AQN-1.
The mannose-binding capability of recombinant wild-type boar spermadhesin AQN-1 and of its site-directed mutants in the highly-conserved region around of the single glycosylation site (asparagine 50) of some spermadhesins, where the carbohydrate binding site has been proposed to be located, was checked using a solid-phase assay and a biotinylated mannose ligand. Substitution of glycine 54 by amino acids bearing an unipolar side chain did not cause significant decrease in the mannose-binding activity. However, amino acids with uncharged polar side chains or having a charged polar side chain abolished the binding of biotinylated mannose to the corresponding AQN-1 mutants. The results suggest that the higher surface accessibility of amino acids possessing polar side chains compared to those bearing nonpolar groups may sterically interfere with monosaccharide binding. The location of the mannose-binding site in AQN-1 appears to be topologically conserved in other heparin-binding boar spermadhesins, i.e., AQN-3 and AWN, but departs from the location of the mannose-6-phosphate-recognition site of PSP-II. This indicates that different spermadhesin molecules have evolved non-equivalent carbohydrate-binding capabilities, which may underlie their distinct patterns of biological activities.
Ekhlasi-Hundrieser
Mahnaz
M
Department of Reproductive Biology, University of Veterinary Medicine Hannover, Foundation, Bünteweg 2, 30559 Hannover, Germany. mahnaz.ekhlasi@tiho-hannover.de
Calvete
Juan J
JJ
Von Rad
Bettina
B
Hettel
Christiane
C
Nimtz
Manfred
M
Töpfer-Petersen
Edda
E
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D008358:Mannose / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry; D017354:Point Mutation / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D029589:Seminal Plasma Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013552:Swine / Q000378:metabolism*
D050505:Mutant Proteins; D011994:Recombinant Proteins; D029589:Seminal Plasma Proteins; C425042:spermadhesin; D008358:Mannose