Analysis of probenazole-responsiveness of rice RPR1 upstream fragments.
RPR1 (rice probenazole-responsive) is a rice gene, the expression of which is responsive to probenazole (PBZ), a synthetic compound that may act as a plant defense activator. It has been shown that RPR1 gene may be involved in disease resistance responses. In this study, a series of amplified fragments from the rice RPR1 promoter region, including 2,416 bp, 1,574 bp, 819 bp, 568 bp and 208 bp fragments upstream to the ATG translation start site, were prepared and linked to the coding region of beta-glucuronidase (GUS) gene. Analysis of GUS gene transient expression in rice calli demonstrated that the 568 bp fragment was sufficient for probenazole responsiveness. Analysis of GUS gene stable expression in Arabidopsis thaliana indicated that the 2,416 bp and 1,574 bp fragments drove GUS expression only in shoot apical meristem and petiole. Identification of these PBZ-responsive fragments provides a basis on which PBZ-inducible gene regulatory systems can be constructed for experimental analysis of gene expression and for field application.
Zhao
Xiang-Hui
XH
Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China.
Yang
Jin-Xiao
JX
Gao
Jiong
J
Zhou
Qian
Q
Kuai
Ben-Ke
BK
D017360:Arabidopsis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D018519:Meristem / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics
D004268:DNA-Binding Proteins; D010940:Plant Proteins; C121428:RPR1 protein, Oryza sativa; D005966:Glucuronidase