title: Structure of a phosphoethanolamine-containing O-polysaccharide of Citrobacter freundii strain PCM 1443 from serogroup O39 and its relatedness to the Klebsiella pneumoniae O1 polysaccharide.
abstract: Lipopolysaccharide was extracted from cells of Citrobacter freundii PCM 1443 from serogroup O39 and degraded by mild acid hydrolysis to give an O-polysaccharide. Based on enzymatic and methylation analyses, along with 1H and 13C nuclear magnetic resonance spectroscopy, it was found that the lipopolysaccharide studied has two different linear polysaccharide chains of d-galactan type containing 3-substituted galactose residues. One of the galactans has the disaccharide repeating units of alpha-D-galactopyranose and beta-D-galactofuranose and the other is comprised of alpha-D-galactopyranose and beta-D-galactopyranose, the latter being substituted in 25% repeats with PEtN at O-6. An immunoblotting assay demonstrated that the lipopolysaccharide of C. freundii PCM 1443 is serologically related to that of Klebsiella pneumoniae O1, which contains the same galactan chains but is devoid of phosphoethanolamine.
authors: L Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla, Wrocław, Poland. katzenel@iitd.pan.wroc.pl
authors: Ewa
authors: E
authors: Katzenellenbogen
authors: Philip V
authors: PV
authors: Toukach
authors: Nina A
authors: NA
authors: Kocharova
authors: Agnieszka
authors: A
authors: Korzeniowska-Kowal
authors: Andrzej
authors: A
authors: Gamian
authors: Alexander S
authors: AS
authors: Shashkov
authors: Yuriy A
authors: YA
authors: Knirel
mesh_terms: D015153:Blotting, Western; D016971:Citrobacter freundii / Q000737:chemistry*; D005685:Galactans / Q000737:chemistry* / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005685:Galactans; D008070:Lipopolysaccharides; D019081:O Antigens; D010714:Phosphatidylethanolamines