Determination of trace glucose and forecast of human diseases by affinity adsorption solid substrate-room temperature phosphorimetry based on triticum vulgaris lectin labeled with dendrimers-porphyrin dual luminescence molecule.
In this paper, 3.5-generation polyamidoamine dendrimers (3.5G-D)-porphyrin (P) dual luminescence molecule (3.5G-D-P) was developed as a new phosphorescence-labeling reagent. Meanwhile, the room temperature phosphorescence (RTP) characteristics of 3.5G-D-P and its product of labeling triticum vulgaris lectin (WGA) on the surface of polyamide membrane (PAM) were studied. Results showed that in the presence of heavy atom perturber LiAc, 3.5G-D and P of 3.5G-D-P molecule could emit strong and stable RTP on the PAM. And the Tween-80 would spike thoroughly the phosphorescence signal of 3.5G-D and P; moreover, specific affinity absorption (AA) reaction between the products (Tween-80-3.5G-D-P-WGA) of WGA labeled with Tween-80-3.5G-D-P and glucose (G) was carried out. The products of the AA reaction could keep good RTP characteristics of 3.5G-D and P dual luminescence molecule, and the DeltaI(p) was linear correlation to the content of G. According to the facts above, a new method of affinity adsorption solid substrate-room temperature phosphorimetry (AA-SS-RTP) for the determination of trace G was established, basing on WGA labeled with Tween-80-3.5G-D-P dual luminescence molecule. The detection limit of this method was 0.13fgspot(-1) (1.7x10(-12)moll(-1), 3.5G-D) and 0.14fgspot(-1) (2.2x10(-12)moll(-1), P). Determination of G in human serum using excitation/emission wavelength of either 3.5G-D or P, the result was coincided with enzyme-linked immunosorbent assay (ELISA). Not only the sensitivity and accuracy of this method were higher, but also the flexibility of AA-SS-RTP was obviously improved and the applicability was wider.
Liu
Jia-Ming
JM
Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, PR China. zzsyliujiaming@163.com
Liu
Zhen-Bo
ZB
Zhu
Guo-Hui
GH
Li
Xue-Lin
XL
Huang
Xiao-Mei
XM
Li
Fei-Ming
FM
Shi
Xiu-Mei
XM
Zeng
Li-Qing
LQ
D000327:Adsorption; D050091:Dendrimers / Q000737:chemistry*; D004194:Disease; D005947:Glucose / Q000032:analysis*; D006801:Humans; D049449:Luminescence; D011166:Porphyrins / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods; D013696:Temperature; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
D050091:Dendrimers; D011166:Porphyrins; D014909:Wheat Germ Agglutinins; D005947:Glucose