HPLC determination of serotonin and its metabolites from human platelet-rich plasma; shift to 5-hydroxytryptophol formation following alcohol consumption.
A sensitive, simple, and reliable high-performance liquid chromatographic method with electrochemical detection is developed for the measurement of four natural products, the serotonin-related indols from human platelet-rich plasma (PRP) using N-methylserotonin as internal standard. Separation of serotonin (5HT), 5-hydroxytryptophan (5HTP), 5-hydroxytryptophol (5HTOL), and 5-hydroxyindole-acetic acid (5HIAA) is carried out on Supelcosil LC-18DB stationary phase. A mixture of 48 mM citric acid, 28 mM sodium phosphate dibasic, 0.027 mM Na2EDTA, and 3% methanol (pH 3.18) serves as the mobile phase. Measurements are carried out at 25 degrees C at Eox=0.65 V. The calibration curves are linear through the range of 10-200 pg/mL. Method validation is performed according to internationally accepted criteria. Blood is collected from healthy controls and schizophrenic subjects. Significantly higher PRP serotonin is measured in schizophrenics; patients with recent alcohol consumption could be characterized with significantly elevated 5HTOL/5HIAA ratio.
Tekes
Kornélia
K
Department of Pharmacodynamics, Semmelweis University, H-1089 Nagyvárad tér Budapest, Hungary. tekkor@net.sote.hu
D006916:5-Hydroxytryptophan / Q000097:blood; D000328:Adult; D000428:Alcohol Drinking / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D006917:Hydroxytryptophol / Q000097:blood* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D012559:Schizophrenia / Q000378:metabolism; D012701:Serotonin / Q000031:analogs & derivatives / Q000097:blood* / Q000378:metabolism; D018709:Statistics, Nonparametric
C008494:N-methylserotonin; D006917:Hydroxytryptophol; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006916:5-Hydroxytryptophan