title: Evidence for a putative flavonoid translocator similar to mammalian bilitranslocase in grape berries (Vitis vinifera L.) during ripening.
abstract: During maturation, Vitis vinifera berries accumulate a large amount of several anthocyanins in the epidermal tissue, whereas their precursors and intermediates are ubiquitously synthesized within the fruit. Up to date, several mechanisms of flavonoid transport at subcellular level have been hypothesized, but it is not possible to identify a general model applicable in every plant tissue and organ. Recently, a putative anthocyanin carrier, homologue to mammalian bilitranslocase (BTL) (TC 2.A.65.1.1), was found in Dianthus caryophyllus petal microsomes. In the present paper, an immunohistochemical and immunochemical analysis, using an antibody raised against a BTL epitope, evidences the expression and function of such a transporter in V. vinifera berries (cv. Merlot). Specific localisations of the putative carrier within berry tissues together with expression changes during different developmental stages are shown. Water stress induces an increase in protein expression in both skin and pulp samples. A bromosulfalein (BSP) uptake activity, inhibitable by the BTL antibody, is detected in berry mesocarp microsomes, with K (m) = 2.39 microM BSP and V (max) = 0.29 micromol BSP min(-1) mg(-1) protein. This BSP uptake is also competitively inhibited by quercetin (K (i) = 4 microM). A putative role for this carrier is discussed in relation to the membrane transport of secondary metabolites.
authors: Department of Biology and Plant Protection, University of Udine, via Cotonificio 108, 33100, Udine, Italy.
authors: E
authors: E
authors: Braidot
authors: E
authors: E
authors: Petrussa
authors: A
authors: A
authors: Bertolini
authors: C
authors: C
authors: Peresson
authors: P
authors: P
authors: Ermacora
authors: N
authors: N
authors: Loi
authors: M
authors: M
authors: Terdoslavich
authors: S
authors: S
authors: Passamonti
authors: F
authors: F
authors: Macrì
authors: A
authors: A
authors: Vianello
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002570:Ceruloplasmin; D005419:Flavonoids / Q000378:metabolism*; D005638:Fruit / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D013997:Time Factors; D014634:Valinomycin / Q000494:pharmacology; D027843:Vitis / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D008565:Membrane Proteins; D010940:Plant Proteins; D014634:Valinomycin; D002570:Ceruloplasmin; C033838:bilitranslocase