Preparative-scale chromatography of ecdysteroids: a class of biologically active steroids.
A simple separation procedure is developed for the isolation of the main phytoecdysteroid 20-hydroxyecdysone from the herb Silene viridiflora. The purification in four steps uses only a simple preparative-scale separation technique (i.e., liquid-liquid extraction, precipitation, solid-phase extraction on octadecyl silica, and crystallization). This procedure is extended using classical normal-phase liquid column chromatography, rotation planar chromatography, and preparative high-performance liquid chromatography for the isolation of the minor ecdysteroids: integristerone A, 26-hydroxypolypodine B, 2-deoxy-20,26-dihydroxyecdysone, and polypodine B. 2-Deoxy-20,26-dihydroxyecdysone is isolated from this species for the first time. The isolation of these ecdysteroids in adequate amounts makes them readily available for insect physiology experiments and for structure-activity relationship studies. The preparative-scale separation work also results in a minor, as yet unknown ecdysteroid.
Toth
Noemi
N
Department of Pharmacognosy, University of Szeged, H-6720 Szeged, Eotovs u. 6, Hungary.
Bathori
Maria
M
D002853:Chromatography, Liquid / Q000379:methods*; D026461:Ecdysteroids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D004441:Ecdysterone / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D029751:Silene / Q000737:chemistry*
D026461:Ecdysteroids; D004441:Ecdysterone