In vivo methods to assess polyadenylation efficiency.
Mammalian gene expression can be regulated through various post-transcriptional events, including altered mRNA stability, translational control, and RNA-processing events such as 3'-end formation or polyadenylation (pA). It has become clear in recent years that pA is governed by several core sequence elements and often regulated by additional auxiliary sequence elements. These regulatory events are frequently not reproducible in in vitro assays. Therefore, in vivo methods to measure mRNA pA were developed to meet this need and are described here.
Hague
Lisa K
LK
Department of Biochemistry and Molecular Biology, New Jersey Medical School and the Graduate School of Biomedical Sciences, Newark, NJ, USA.
Hall-Pogar
Tyra
T
Lutz
Carol S
CS
D016375:Antisense Elements (Genetics); D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D026723:Polyadenylation; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012260:Ribonucleases; D014158:Transcription, Genetic; D014162:Transfection
D016375:Antisense Elements (Genetics); D012333:RNA, Messenger; D008156:Luciferases; D012260:Ribonucleases