Rapid species identification of cooked poisonous mushrooms by using real-time PCR.
Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.
Maeta
Kazuhiko
K
The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-cho Minami, Tottori-shi, Tottori 680-8553, Japan.
Ochi
Tomoya
T
Tokimoto
Keisuke
K
Shimomura
Norihiro
N
Maekawa
Nitaro
N
Kawaguchi
Nobuhisa
N
Nakaya
Makoto
M
Kitamoto
Yutaka
Y
Aimi
Tadanori
T
D000363:Agaricales / Q000145:classification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D009145:Mushroom Poisoning / Q000517:prevention & control*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D004271:DNA, Fungal