Quantitative determination of fluorinated caffeic acid phenethyl ester derivative from rat blood plasma by liquid chromatography-electrospray ionization tandem mass spectrometry.
The quantitative determination of caffeic acid phenethyl ester (CAPE) and its fluorinated derivative (FCAPE) from rat plasma using ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) is reported. CAPE and FCAPE were extracted using ethyl acetate in the presence of methyl caffeate (MC) as internal standard. Separation was achieved using a C(18) column (2.1 mm x 50 mm, 1.7 microm) and gradient elution with water and acetonitrile containing 0.2% and 0.1% formic acid, respectively. A non-linear response over a broad concentration range (1-1000 ng/ml, r(2)>0.995 using a quadratic regression model and 1/concentration weighting) was obtained. The inter-day and intra-day variability for CAPE and FCAPE were found to be less than 14.2% and 9.5%, respectively. Data are presented to illustrate the practicality of the method for the pharmacokinetic evaluation of CAPE and FCAPE after intravenous administration to rats.
Wang
Xinyu
X
Pharmaceutics Division, College of Pharmacy, University of Texas at Austin, Austin, TX 78712, United States.
Pang
Jihai
J
Newman
Robert A
RA
Kerwin
Sean M
SM
Bowman
Phillip D
PD
Stavchansky
Salomon
S
D000818:Animals; D002109:Caffeic Acids / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005461:Fluorine / Q000737:chemistry*; D008297:Male; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002109:Caffeic Acids; D005461:Fluorine; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol