title: Affordable and sensitive determination of artemisinin in Artemisia annua L. by gas chromatography with electron-capture detection.
abstract: Artemisinin demand has increased sharply since the World Health Organization recommended its use as part of the artemisinin combination therapies in 2001. The area for the crop cultivation has expanded in Africa and Asia and simpler and affordable methods for artemisinin analysis are needed for crop quality control. This work presented a novel chromatographic method of artemisinin analysis using gas chromatography with electron-capture detection. The sample extraction and preparation involved a single-solvent one-step extraction, with samples being analyzed in the extraction solvent directly after extraction. This method was accurate and reproducible with over 97% recoveries. The limit of detection was less than 3 microg/mL and the limit of quantification was less than 9 microg/mL, allowing samples as low as 100mg dry weight to be analyzed for artemisinin. The method can be applied to quality control of commercial plant extracts and to artemisinin-derived pharmaceuticals.
authors: Natural Products Research Center, Hunan Agricultural University, Changsha 410128, China.
authors: Shuoqian
authors: S
authors: Liu
authors: Na
authors: N
authors: Tian
authors: Zhonghua
authors: Z
authors: Liu
authors: Jianan
authors: J
authors: Huang
authors: Juan
authors: J
authors: Li
authors: Jorge F S
authors: JF
authors: Ferreira
mesh_terms: D019306:Artemisia / Q000737:chemistry*; D037621:Artemisinins / Q000032:analysis*; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D037621:Artemisinins; C031327:artemisinin