Chelatable zinc modulates excitability and seizure duration in the amygdala rapid kindling model.
Zinc is present in high concentration in many structures of the limbic circuitry, however the role of zinc as a neuromodulator in such synapses is still uncertain. In this work, we verified the effects of zinc chelation in an animal model of epileptogenesis induced by amygdala rapid kindling. The basolateral amygdala was electrically stimulated ten times per day for 2 days. A single stimulus was applied on the third day. Stimulated animals received injections of PBS or the zinc chelator diethildythiocarbamate acid (DEDTC) before each stimulus series. Animals were monitored with video-EEG and were perfused 3h after the last stimulus for subsequent neo-Timm and Fluoro-Jade B analysis. Zinc chelation decreased the duration of both behavioral seizures and electrical after-discharges, and also decreased the EEG spikes frequency, without changing the progression of behavioral seizure severity. These results indicate that the zinc ion may have a facilitatory role during kindling progression.
Foresti
Maira Licia
ML
Neurology, Psychiatry and Medical Psychology Department, University of São Paulo, Ribeirão Preto, SP, Brazil.
Arisi
Gabriel Maisonnave
GM
Fernandes
Artur
A
Tilelli
Cristiane Queixa
CQ
Garcia-Cairasco
Norberto
N
D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D004050:Ditiocarb / Q000494:pharmacology; D004569:Electroencephalography / Q000187:drug effects; D004594:Electrophysiology; D005452:Fluoresceins; D007150:Immunohistochemistry; D007696:Kindling, Neurologic / Q000187:drug effects / Q000502:physiology*; D016014:Linear Models; D008297:Male; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000145:classification / Q000503:physiopathology*; D015032:Zinc / Q000502:physiology*
D002614:Chelating Agents; D005452:Fluoresceins; D009930:Organic Chemicals; C435731:fluoro jade; D004050:Ditiocarb; D015032:Zinc