title: Study on chiral resolution of three beta-blockers by affinity electrokinetic chromatography.
abstract: The chiral resolution of three beta-blockers including propranolol, pindolol and oxprenolol, was studied by affinity electrokinetic chromatography. The effect of various chiral selectors and some key parameters including buffer pH, buffer concentration, capillary temperature and applied voltage were carefully studied, respectively. At optimum condition, based on the signal-to-noise ratio of 3, the detection limits for the simple resolution and chiral resolution were found to be 1.0x10(-5) and 4.0 x 10(-5)M, respectively. In addition, the interactions of these beta-blockers with bovine serum albumin (BSA) were studied and the binding constant (K(a)) between BSA and each of beta-blockers were calculated. Based on linear correlation coefficient, it can be concluded that the binding ratio of pindolol (oxprenolol) combining with BSA is 1:1, and that the binding number of propranolol interacting with BSA deviates one.
authors: Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
authors: Guoqiang
authors: G
authors: Yang
authors: Yifang
authors: Y
authors: Zhao
authors: Meixian
authors: M
authors: Li
authors: Zhiwei
authors: Z
authors: Zhu
authors: Qiankun
authors: Q
authors: Zhuang
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D004563:Electrochemistry; D015394:Molecular Structure; D010096:Oxprenolol / Q000737:chemistry; D010869:Pindolol / Q000737:chemistry; D011433:Propranolol / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000319:Adrenergic beta-Antagonists; D012710:Serum Albumin, Bovine; D010096:Oxprenolol; D011433:Propranolol; D010869:Pindolol