title: Lignin peroxidase from Streptomyces viridosporus T7A: enzyme concentration using ultrafiltration.
abstract: It is well known that lignin degradation is a key step in the natural process of biomass decay whereby oxidative enzymes such as laccases and high redox potential ligninolytic peroxidases and oxidases play a central role. More recently, the importance of these enzymes has increased because of their prospective industrial use for the degradation of the biomass lignin to increase the accessibility of the cellulose and hemicellulose moieties to be used as renewable material for the production of fuels and chemicals. These biocatalysts also present potential application on environmental biocatalysis for the degradation of xenobiotics and recalcitrant pollutants. However, the cost for these enzymes production, separation, and concentration must be low to permit its industrial use. This work studied the concentration of lignin peroxidase (LiP), produced by Streptomyces viridosporus T7A, by ultrafiltration, in a laboratory-stirred cell, loaded with polysulfone (PS) or cellulose acetate (CA) membranes with molecular weight cutoffs (MWCO) of 10, 20, and 50 KDa. Experiments were carried out at 25 degrees C and pH 7.0 in accordance to the enzyme stability profile. The best process conditions and enzyme yield were obtained using a PS membrane with 10 KDa MWCO, whereby it was observed a tenfold LiP activity increase, reaching 1,000 U/L and 90% enzyme activity upholding.
authors: Departamento de Bioquímica, IQ, CT, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. ledagott@gmail.com
authors: Leda M F
authors: LM
authors: Gottschalk
authors: Elba P S
authors: EP
authors: Bon
authors: Ronaldo
authors: R
authors: Nobrega
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000201:enzymology*; D013379:Substrate Specificity; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D010544:Peroxidases; C042858:lignin peroxidase