New insights into a bacterial metabolic and detoxifying association responsible for the mineralization of the thiocarbamate herbicide molinate.
A novel pathway of molinate mineralization promoted by a defined mixed culture composed of five bacteria (named ON1 to ON5) was proposed previously. Evidence was obtained of a metabolic association between Gulosibacter molinativorax ON4(T), capable of molinate breakdown, and the remaining bacteria. In the present study, the role of each isolate in that metabolic association was further explored and the possible synergistic effect of all the bacterial isolates for the stability of the mixed culture is discussed. The cleavage of the molinate thioester bond, whether occurring under aerobic or anaerobic conditions, releases ethanethiol (S-ethyl moiety) and an azepane moiety derivative, identified as azepane-1-carboxylic acid. This azepane moiety is degraded, in the presence of oxygen, by Pseudomonas strains ON1 and ON3 and G. molinativorax ON4(T). Ethanethiol, which inhibits G. molinativorax ON4(T), is consumed by Pseudomonas strain ON1 and Stenotrophomonas maltophilia ON2. Although a two-member mixed culture of G. molinativorax ON4(T) and Pseudomonas strain ON1 was able to promote the aerobic mineralization of molinate, after 20 successive transfers of the five-member mixed culture in mineral medium with molinate, none of these isolates were lost. The results obtained indicate that the whole mixed culture may have a higher fitness than the two-member culture, even when the basic degradative and cross-protection functions are assured.
Barreiros
Luisa
L
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal.|LEPAE - Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal.
Fernandes
Ana
A
LEPAE - Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal.
Ferreira
António C Silva
ACS
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal.
Pereira
Helena
H
Instituto de Tecnologia Química e Biológica, Av. da República - EAN, 2780-157 Oeiras, Portugal.
Bastos
Margarida M S M
MMSM
LEPAE - Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal.
Manaia
Célia M
CM
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal.
Nunes
Olga C
OC
LEPAE - Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal.
D000192:Actinomycetales / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D001381:Azepines / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006540:Herbicides / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D015394:Molecular Structure; D011549:Pseudomonas / Q000378:metabolism*; D020615:Stenotrophomonas maltophilia / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013859:Thiocarbamates / Q000378:metabolism*
D001381:Azepines; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D006540:Herbicides; D013438:Sulfhydryl Compounds; D013859:Thiocarbamates; C009821:molinate; C007638:ethanethiol