title: Distribution of Salmonella pathogenicity island (SPI)-8 and SPI-10 among different serotypes of Salmonella.
abstract: Many virulence phenotypes of Salmonella enterica are encoded by genes located on pathogenicity islands. Based on genome analysis, it is predicted that Salmonella pathogenicity island (SPI)-8 is restricted to Salmonella serovars Typhi and Paratyphi A, and SPI-10 to Salmonella serovars Typhi, Paratyphi, Enteritidis, Dublin and Gallinarum. This study was conducted to investigate the distribution of SPI-8 and SPI-10 among Salmonella isolates from sprouts, fish, water and blood. A total of 110 Salmonella isolates and 6 Salmonella serovars from the Microbial Type Culture Collection, Chandigarh, India, were screened. All isolates belonging to Salmonella serovars Washington, Enteritidis and Paratyphi A had both SPI-8 and SPI-10. All Salmonella serovar Typhi isolates from water and blood had both SPI-8 and SPI-10, whereas isolates from fish contained only SPI-8. SPI-8 and SPI-10 were also detected in only 3 out of 42 isolates belonging to Salmonella serovar Typhimurium. Both SPI-8 and SPI-10 were absent in Salmonella serovars Worthington, Dublin, Paratyphi B and Paratyphi C. These results contradict the predictions from Salmonella genome sequences available in GenBank and indicate that SPI-8 and SPI-10 are widely distributed among Salmonella serovars and that virulence factors other than those on SPI-8 and SPI-10 may be responsible for host specificity. This is the first report on the distribution of SPIs in Salmonella isolates from India.
authors: Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
authors: Sunil D
authors: SD
authors: Saroj
authors: Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
authors: R
authors: R
authors: Shashidhar
authors: Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
authors: Manisha
authors: M
authors: Karani
authors: Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
authors: Jayant R
authors: JR
authors: Bandekar
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D044404:Genomic Islands / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D012480:Salmonella Infections / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013045:Species Specificity; D014675:Vegetables / Q000382:microbiology; D014774:Virulence; D014871:Water Microbiology