Surface chemistry and in situ spectroscopy of a lysozyme langmuir monolayer.
Surface pressure and surface potential-area isotherms were used to characterize a lysozyme Langmuir monolayer. The compression-decompression cycles and stability measurements showed a homogeneous and stable monolayer at the air-water interface. Salt concentration in the subphase and pH of the subphase were parameters controlling the homogeneity and stability of the Langmuir monolayer. In situ UV-vis and fluorescence spectroscopies were used to verify the homogeneity of the lysozyme monolayer and to identify the chromophore residues in the lysozyme. Optimal experimental conditions were determined to prepare a homogeneous and stable lysozyme Langmuir monolayer.
Thakur
Garima
G
Department of Chemistry, 1301 Memorial Drive, University of Miami, Coral Gables, Florida 33146, USA.
Wang
Chengshan
C
Leblanc
Roger M
RM
D000818:Animals; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011189:Potassium Chloride; D011312:Pressure; D013057:Spectrum Analysis; D013499:Surface Properties; D013696:Temperature
D011189:Potassium Chloride; D009113:Muramidase