The effect of an anti-inflammatory pentapeptide produced by Entamoeba histolytica on gene expression in the U-937 monocytic cell line.
OBJECTIVE
Evaluate the Monocyte Locomotion Inhibitory Factor (MLIF) effect upon the expression of genes encoding human cytokines, receptors and related factors in the human cell line U-937. MLIF (Met-Gln-Cys-Asn-Ser) is an anti-inflammatory pentapeptide produced by Entamoeba histolytica that inhibits many human monocyte functions.


METHODS
U-937 cell line cultured (24 hrs/RPMI). RNA extracted by Trizol method. 385 genes were analyzed on microarray membranes, complement by real-time RT-PCR and protein expression of some affected genes.


RESULTS
MLIF had a preferentially inhibitory effect on gene expression; four genes were over-expressed and 13 underexpressed in MILF vs. simple medium - constitutive expression. Three genes are over-expressed and 19 under-expressed in MLIF/PMA vs. PMA - induced expression.


CONCLUSIONS
Many modified genes are products regulated by the Nuclear Factor-kappaB and Mitogen Activated Protein Kinase pathways, suggesting MLIF involvement with these two major pathways for the modulation of the inflammation and immune responses.
Silva-García
R
R
Centro Médico Nacional Siglo XXI, Unidad de Investigación Médica en Inmunología, Instituto Mexicano del Seguro Social, Hospital de Pediatría, Avenida Cuauhtémoc 330, Col. Doctores, C P 06720, México, D F, México.
Estrada-García
I
I
Ramos-Payán
R
R
Torres-Salazar
A
A
Morales-Martínez
M E
ME
Arenas-Aranda
D
D
Giménez-Scherer
J A
JA
Blanco-Favela
F
F
Rico-Rosillo
M G
MG
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology*; D020298:U937 Cells
D016207:Cytokines; D016328:NF-kappa B; D009842:Oligopeptides; C430647:monocyte locomotion inhibitory factor; D020929:Mitogen-Activated Protein Kinase Kinases