Intestinal oleoyl-estrone esterase activity in the Wistar rat.
Low-dose oral oleoyl-estrone (OE) (i.e. in dairy products) is hydrolysed to estrone, which promotes growth and fat deposition. However, pharmacological doses of OE are absorbed largely intact and elicit fat losses. Thus, in order to find out how the intestine handles OE, esterase activity (at pH 5, 7 or 8) was measured in rat stomach, duodenum, jejunum, ileum, cecum, large intestine, and liver using OE as substrate. There were no sex-related differences. Pure pancreatic cholesterol-ester esterase hydrolysed OE even in the absence of taurocholate. The differences in the pH-related activity distribution pattern and selective inhibition and taurocholate dependence show that, in addition to the luminal (i.e. pancreatic) cholesterol-ester esterase, other esterases hydrolyse OE; these combined activities may be sufficient to rapidly dispose of pharmacological doses of OE. Female rats received a tritium-labeled OE gavage; the luminal and tissue label content were measured up to 24 h. The high retention of label in the stomach suggest that this may be a significant site of absorption. The rapid decrease of label in intestinal lumen (and rat tissues) shortly after the administration, hint at rapid absorption and disposal. In conclusion, the high OE-esterase activity and early absorption of OE are indicative of upper gastro-intestinal tract absorption skipping most of the medium-tract esterases.
Serrano-Muñoz
M
M
Department of Nutrition and Food Science, Faculty of Biology, University of Barcelona, Barcelona, Spain.
Grasa
M M
MM
González-Martínez
D
D
Cabot
C
C
Fernández-López
J A
JA
Alemany
M
M
D000818:Animals; D001835:Body Weight; D002788:Cholesterol Esters / Q000378:metabolism; D004970:Estrone / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000201:enzymology*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012727:Sex Characteristics; D002787:Sterol Esterase / Q000378:metabolism*; D013552:Swine; D013997:Time Factors; D014018:Tissue Distribution
D002788:Cholesterol Esters; D009829:Oleic Acids; D004970:Estrone; C014409:cholesteryl oleate; C108709:oleoyl-estrone; D002787:Sterol Esterase