Cloning and characterization of an rRNA methyltransferase from Sorangium cellulosum.
A locus (kmr) responsible for aminoglycosides-resistance of Sorangium cellulosum was cloned and characterized in Myxococcus xanthus. The gene kmr encodes a putative rRNA methyltransferase. Expression of the complete ORF endowed the Myxococcus transformants with the resistance to aminoglycosidic antibiotics of kanamycin, apramycin, gentamycin, neomycin, and tobramycin at an extraordinary high-level (MIC, higher than 500 microg/ml). However, the gene did not function in Escherichia coli cells. In Sorangium genome, the gene kmr was followed by a putative integrase gene, and was highly homologous in different Sorangium strains. The Sorangium rRNA methyltransferase sequence was in low similarity to the reported 16S rRNA methyltransferases, and their resistance spectrums were also different. The results indicate that the rRNA methyltransferase (Kmr) in Sorangium strains is a new member of the rRNA methyltransferases family.
Zhao
Jing-Yi
JY
State Key Laboratory of Microbial Technology, College of Life Science, Shandong University, Jinan 250100, People's Republic of China.
Xia
Zhi-Jie
ZJ
Sun
Xia
X
Zhong
Li
L
Jiang
De-Ming
DM
Liu
Hong
H
Wang
Jing
J
Qin
Zhong-Jun
ZJ
Li
Yue-Zhong
YZ
D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D007613:Kanamycin Resistance / Q000235:genetics*; D008780:Methyltransferases / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D009231:Myxococcales / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D016941:Myxococcus xanthus / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000378:metabolism*
D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D008780:Methyltransferases