Cytotoxic constituents from Podocarpus fasciculus.
A new diterpene, 16-hydroxy communic acid (1), along with thirty one known compounds including five norditerpenes (2-6), twenty two flavonoids containing four biflavonoids (7-10), nine monoflavonoids (11-19) and nine flavanoid glycosides (20-28), as well as four phenolic constituents (29-32) were isolated from the 95% ethanolic extract of Podocarpus fasciculus. The structure of 1 was elucidated using spectral methods. Of these isolates, nagilactone C (2) showed the most significant inhibitory effects against DLD cells (human colon carcinoma) (ED(50)=2.57 microg/ml) and compounds 7, 8, 10, 11, and 12 had moderate cytotoxic activity against human KB (human oral epithelium carcinoma), Hela (human cervical carcinoma), Hepa (human hepatoma), DLD (colon carcinoma), and A-549 (human lung carcinoma) tumor cell lines. Preliminary structure-activity relationship studies of the isolated diterpenoids and biflavonoids are discussed.
Kuo
Yu-Jen
YJ
National Research Institute of Chinese Medicine, Taipei, Taiwan, ROC.
Hwang
Shy-Yuan
SY
Wu
Ming-Der
MD
Liao
Chia-Ching
CC
Liang
Yu-Han
YH
Kuo
Yao-Haur
YH
Ho
Hsiu-O
HO
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology; D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007202:Indicators and Reagents; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013778:Tetrazolium Salts; D013844:Thiazoles; D064028:Tracheophyta / Q000737:chemistry*
C529361:16-hydroxycommunic acid; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue