title: Biological effects of a sulfated-polysaccharide isolated from the marine red algae Champia feldmannii.
abstract: Sulfated-polysaccharides are exploited as antithrombotic and anticoagulant agents and suggested to be immunostimulants. The sulfated-polysaccharide isolated from the red-marine-algae Champia feldmannii (Cf-PLS) was purified by ion exchange chromatography and tested in experimental protocols of coagulation, inflammation (in Wistar rats) and nociception (in Swiss mice). Cf-PLS was tested i.v. for its anti-inflammatory activity in the paw-edema induced by classical inflammatory stimuli and s.c. for its pro-inflammatory activity in the paw-edema and peritonitis models. The anticoagulant activity was evaluated by the test of partial thromboplastin activation time (aPTT) and the antinociceptive effect in the writhing-test. Cf-PLS was not anti-inflammatory, but rather induced maximal edematogenic activity at 0.9 mg/kg (1.01+/-0.030 x 0.06+/-0.03 ml) compared to controls (0.06+/-0.03 ml), increased vascular-permeability (38.44+/-12.63 x 11.29+/-3.91 microg/g) and stimulated neutrophil migration (3.348+/-295 x 307+/-99 cells/microl) 1 h after injection. Cf-PLS was also antinociceptive (6.6+/-1.28 x 33+/-1.44 writhes) and extended human plasma coagulation time by 3 times. Our data suggest that this molecule may be an important immunostimulant.
authors: Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana 1700, 60740-000, Fortaleza-Ceará-Brasi. assreuy@uece.br
authors: Ana Maria Sampaio
authors: AM
authors: Assreuy
authors: Daniel Magalhães
authors: DM
authors: Gomes
authors: Michelle Soares Josino
authors: MS
authors: da Silva
authors: Valeska Martins
authors: VM
authors: Torres
authors: Rômmulo Celly Lima
authors: RC
authors: Siqueira
authors: Alana de Freitas
authors: Ade F
authors: Pires
authors: David Neil
authors: DN
authors: Criddle
authors: Nylane Maria Nunes
authors: NM
authors: de Alencar
authors: Benildo Sousa
authors: BS
authors: Cavada
authors: Alexandre Holanda
authors: AH
authors: Sampaio
authors: Wladimir Ronald Lobo
authors: WR
authors: Farias
mesh_terms: D019342:Acetic Acid; D018712:Analgesics, Non-Narcotic; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D001938:Brazil; D054442:Cell Migration Assays, Macrophage; D002852:Chromatography, Ion Exchange; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000187:drug effects; D005528:Foot / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control; D008297:Male; D010147:Pain Measurement / Q000187:drug effects; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D000461:Rhodophyta / Q000737:chemistry*
chemical_list: D018712:Analgesics, Non-Narcotic; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D011134:Polysaccharides; D019342:Acetic Acid