[Analysis of human normal prostate cells membrane proteins by shotgun-MS coupled with laser capture microdissection].
OBJECTIVE
To analyze of membrane proteins of human normal prostate epithelial cells.


METHODS
Laser capture microdissection (LCM) technique was utilized to obtain the epithelial cells of human normal prostate. Shotgun-MS was used to generate protein profiles in the epithelial cells of human normal prostate.


RESULTS
LCM technique successfully separated the normal prostate epithelial cells with homogeneity more than 95%. Under a stringent filter condition (charge +1, Xcorr >/= 1.9; charge +2, Xcorr >/= 2.2; charge +3, Xcorr >/= 3.75; DelCN >/= 0.1), 1164 proteins were identified in the human normal prostate cells, of which 799 had a gene ontology annotation (GOA) indicating a cellular component, others have no GOA terms. Among the GOA terms, 377 (49.15%) were known membrane proteins or membrane associated proteins. In addition to the proteins known to be associated with the membrane, a significant number of novel proteins had also been identified, including several hypothetical proteins and cDNA sequences.


CONCLUSIONS
Shotgun-MS technique coupled with LCM effectively analyzes the proteins of human normal prostate cells, thus helping perfect the complete protein profiles of human normal prostate cells.
Li
Qun
Q
Key Laboratory of Pathobiology, Ministry of Education, Basic Medical School, Jilin University, Changchun 130021, China.
Li
Yi-Lei
YL
Li
Wei
W
Yang
Xin-Yu
XY
Wang
Yi-Mei
YM
Li
Yu-Lin
YL
D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007834:Lasers; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis*; D042282:Microdissection / Q000379:methods; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*
D008565:Membrane Proteins