Plasmodium falciparum dolichol phosphate mannose synthase represents a novel clade.
Dolichol phosphate mannose synthase (DPM) catalyzes the reaction between dolichol phosphate (Dol-P) and guanosine diphosphate mannose (GDP-Man) to form dolichol-phosphate-mannose (Dol-P-Man). This molecule acts as mannose donor for N-glycosylation and glycosylphosphatidylinositol (GPI) biosynthesis. The Plasmodium falciparum DPM1 (Pfdpm1) possesses a single predicted transmembrane region near the N-, but not the C-terminus. Here we show that the cloned Pfdpm1 gene failed to complement a Saccharomyces cerevisiae mutant indicating that the parasite gene does not belong to the baker's yeast group, as was previously assumed. Furthermore, Pfdpm1 was unable to complement a mouse mutant deficient in DPM but efficiently complements the Schizosaccharomyces pombe fission yeast mutant, indicating a difference between fission yeast and mammalian DPM genes. Therefore, we reanalyzed the hydrophobicity scales of all known DPMs and consequently reclassify the DPM clade into six major novel subgroups. Furthermore, we show that Pfdpm1 represents a unique enzyme among these subgroups.
Shams-Eldin
Hosam
H
Institute for Virology, Laboratory of Parasitology, Medical Centre for Hygiene and Medical Microbiology, Philipps University, Hans-Meerwein-Str. 2, 35043 Marburg, Germany. shamseld@staff.uni-marburg.de
de Macedo
Cristiana Santos
CS
Niehus
Sebastian
S
Dorn
Caroline
C
Kimmel
Jürgen
J
Azzouz
Nahid
N
Schwarz
Ralph T
RT
D000818:Animals; D003001:Cloning, Molecular; D005816:Genetic Complementation Test; D006801:Humans; D008364:Mannosyltransferases / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D010963:Plasmodium falciparum / Q000201:enzymology*; D015800:Protozoan Proteins / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics
D015800:Protozoan Proteins; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase