title: p53 and p21/WAF-1 overexpressions in prostatic adenocarcinoma.
abstract: OBJECTIVE
  This study was carried out to determine the role of p53 and p21 in the pathogenesis of prostatic adenocarcinoma and their association with tumour grade.
  
  
  METHODS
  Sixty-seven histologically confirmed prostatic adenocarcinoma cases collected from Hospital Universiti Kebangsaan Malaysia and General Hospital Kuala Lumpur were studied. The formalin-fixed paraffin-embedded tissues were stained with monoclonal antibody p53 (clone DO-7) and p21 (clone SX II8) using standard avidin-biotin complex immunohistochemistry (IHC) after microwave antigen retrieval. The adenocarcinomas were graded histologically according to Gleason score: low grade = score 2-4, intermediate grade = score 5-7, high grade = score 8-10. Histological grade was analysed against IHC expressions of p53 and p21 and against patient age and ethnicity.
  
  
  RESULTS
  IHC positivity for p53 was expressed in 1/2 (50%) low grade, 14/33 (42%) intermediate grade, and 21/32 (66%) high grade tumours. p21 was expressed in 0/2 low grade, 16/33 (48%) intermediate grade and 15/32 (47%) high grade tumours. p53 and p21 expressions did not show statistically significant correlation with the different grades of prostatic adenocarcinoma or with each other (p = 0.42). There was no correlation between tumour grade and age or ethnicity.
  
  
  CONCLUSIONS
  Although the p53 positivity rate was higher in high-grade prostate adenocarcinoma, this was not statistically significant. We found that a combined expression of p21 and p53 was not correlated with tumour grade. We could not confirm that p21 expression was dependent on p53 expression.
authors: Department of Pathology, Faculty of Medicine, National University of Malaysia. muhaizan@mail.hukm.ukm.my
authors: Wan Mustaffa
authors: WM
authors: Wan Muhaizan
authors: Puaat Khalid
authors: PK
authors: Ahmad
authors: Koon Seng
authors: KS
authors: Phang
authors: Talib
authors: T
authors: Arni
mesh_terms: D000230:Adenocarcinoma / Q000208:ethnology / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000208:ethnology; D014408:Biomarkers, Tumor / Q000378:metabolism; D002681:China / Q000208:ethnology; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D006801:Humans; D008296:Malaysia / Q000208:ethnology; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000208:ethnology / Q000378:metabolism* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016159:Tumor Suppressor Protein p53