Stable expression of green fluorescent protein mediated by GCV in Giardia canis.
Giardia canis can be infected with a double-stranded RNA virus, that is giardiavirus (G. canis virus, GCV). In this study, green fluorescent protein (GFP) was stably expressed in G. canis mediated by GCV. The plasmid pNEO/GDH/MCS/GFP, containing the neomycin phosphotransferase (NEO) encoding region flanked by the 636 nt of 5'-terminus and the 2174 nt of 3'-terminus from GCV positive strand RNA, was constructed by inserting GFP gene into downstream from the NEO gene and glutamate dehydrogenase (GDH) 5'-terminus uncoding regions on a single plasmid, and its in vitro transcript was introduced into GCV-infected G. canis by electroporation. The transfectants expressed GFP persistently under G418 selection. This stable transfection system should provide a valuable tool for genetic study of G. canis.
Liu
Chengwu
C
College of Animal Science and Veterinary Medicine, JiLin University, 5333 Xi'an Road, Changchun, China.
Li
Jianhua
J
Zhang
Xichen
X
Liu
Quan
Q
Liu
Hui
H
Gong
Pengtao
P
Zhang
Guocai
G
Yao
Longquan
L
Zhang
Xinxin
X
D000818:Animals; D004285:Dogs / Q000469:parasitology*; D018274:Electroporation; D005786:Gene Expression Regulation; D015967:Gene Expression Regulation, Viral; D005872:Giardia / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000821:virology; D019198:Giardiavirus / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D019868:Kanamycin Kinase / Q000235:genetics / Q000378:metabolism; D010274:Parasitology / Q000379:methods; D014162:Transfection / Q000379:methods*
D049452:Green Fluorescent Proteins; D019868:Kanamycin Kinase