Sample preparation of culture medium from Madin-Darby canine kidney cells.
A reproducible, standardized and simple sample preparation methodology is the key to successful two-dimensional gel electrophoresis (2-DE). This chapter describes step-by-step the sample preparation of culture medium from Madin-Darby canine kidney (MDCK) cells. Tips and tricks are given to circumvent possible pitfalls.
Ambort
Daniel
D
University of Berne, Berne, Switzerland.
Lottaz
Daniel
D
Sterchi
Erwin
E
D000818:Animals; D002460:Cell Line; D003470:Culture Media; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional; D007668:Kidney / Q000166:cytology*; D014461:Ultracentrifugation; D014462:Ultrafiltration
D003470:Culture Media