title: Basement membranes in skin are differently affected by lack of nidogen 1 and 2.
abstract: Nidogens have been proposed to play a key role in basement membrane (BM) formation. However, recent findings using genetic approaches and organotypic coculture models demonstrated distinct tissue requirements thus changing the classical view of BM assembly. Toward this end, we have analyzed the dermo-epidermal junction and the microvasculature in skin of nidogen-deficient mice for their BM composition and structural assembly. Histology of nidogen double-null embryos at embryonic day (E)18.5 revealed overall normal skin morphology with a regularly differentiated epidermis. However, in the dermis, numerous erythrocytes had extravasated out of the microvasculature. Residual composition and ultrastructure of the dermo-epidermal BM are not altered in the absence of nidogens, demonstrating that the deposition of laminin, collagen IV, and perlecan occurs and allows cutaneous BM formation. In contrast, in capillaries, BM formation is severely impaired in the absence of nidogens, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411. Ultrastructure revealed thin fragile walls in the small blood vessels next to the epidermis, completely lacking a distinct endothelial BM. In summary, our results indicate that in skin the laminin composition of the various BMs determines whether nidogens are required for their assembly and stabilization.
authors: Department of Dermatology, University of Cologne, Cologne, Germany.
authors: Sharada
authors: S
authors: Mokkapati
authors: Anke
authors: A
authors: Baranowsky
authors: Nicolae
authors: N
authors: Mirancea
authors: Neil
authors: N
authors: Smyth
authors: Dirk
authors: D
authors: Breitkreutz
authors: Roswitha
authors: R
authors: Nischt
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002135:Calcium-Binding Proteins; D002196:Capillaries / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D015815:Cell Adhesion Molecules; D024141:Collagen Type IV / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012867:Skin / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D015815:Cell Adhesion Molecules; D024141:Collagen Type IV; D019812:Heparan Sulfate Proteoglycans; D007797:Laminin; D008562:Membrane Glycoproteins; C112846:Nid2 protein, mouse; C029967:nidogen; C071927:perlecan