title: In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells.
abstract: Histone chaperones have been implicated in nucleosome assembly and disassembly as well as histone modification. ASF1 is a highly conserved histone H3/H4 chaperone that synergizes in vitro with two other histone chaperones, chromatin assembly factor 1 (CAF-1) and histone repression A factor (HIRA), in DNA synthesis-coupled and DNA synthesis-independent nucleosome assembly. Here, we identify mutants of histones H3.1 and H3.3 that are unable to interact with human ASF1A and ASF1B isoforms but that are still competent to bind CAF-1 and HIRA, respectively. We show that these mutant histones are inefficiently deposited into chromatin in vivo. Furthermore, we found that both ASF1A and ASF1B participate in the DNA synthesis-independent deposition of H3.3 in HeLa cells, thus highlighting an unexpected role for ASF1B in this pathway. This pathway does not require interaction of ASF1 with HIRA. We provide the first direct determination that ASF1A and ASF1B play a role in the efficiency of nucleosome assembly in vivo in human cells.
authors: CEA, SBIGeM-Bât. 142, 91191 Gif-sur-Yvette Cedex, France.
authors: Angélique
authors: A
authors: Galvani
authors: Régis
authors: R
authors: Courbeyrette
authors: Morgane
authors: M
authors: Agez
authors: Françoise
authors: F
authors: Ochsenbein
authors: Carl
authors: C
authors: Mann
authors: Jean-Yves
authors: JY
authors: Thuret
mesh_terms: D001120:Arginine / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D016193:G1 Phase; D006367:HeLa Cells; D056488:Histone Chaperones; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009707:Nucleosomes / Q000378:metabolism*; D016196:S Phase; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C491641:ASF1A protein, human; C491640:ASF1B protein, human; C489780:CNOT8 protein, human; D018797:Cell Cycle Proteins; D002843:Chromatin; C086057:HIRA protein, human; D056488:Histone Chaperones; D006657:Histones; D018832:Molecular Chaperones; D009707:Nucleosomes; D014157:Transcription Factors; D001120:Arginine