Histological evaluation of biocompatibility of lyophilized bovine pericardium implanted subcutaneously in rats.
This article aims at investigating in vivo evaluation of lyophilization procedure on the biocompatibility of bovine pericardium treated with glutaraldehyde (GA). The bovine pericardium was fixed with 0.5% glutaraldehyde during 10 days and preserved in 4% formaldehyde (FA). Two groups of samples were prepared from treated membranes: Group 1, nonlyophilized samples and Group 2, lyophilized samples. Male Sprague-Dawley rats (4 weeks after birth) were anesthetized (pentobarbital sodium 25 mg/kg of body weight) and in each one were implanted subcutaneously in the dorsal region a sample from Group 1 and another from Group 2. These samples were explanted after 30 days for histological analysis. No intercurrences took place after the surgery. No differences (P > 0.05) in the calcification, granulomatous reaction, mononuclear infiltration, and granulation tissue development was observed between both groups. The implanted lyophilized samples presented a trend for a reduced inflammatory reaction. Lyophilization of the bovine pericardium does not seem to increase the above listed tissue reaction.
Maizato
Marina J S
MJ
Bioengineering Division-Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil. biomarina@incor.usp.br
Pires
Mara D
MD
Canzian
Mauro
M
Higa
Olga Z
OZ
Pitombo
Ronaldo N M
RN
Leirner
Adolfo A
AA
D000818:Animals; D001705:Bioprosthesis; D002114:Calcinosis / Q000473:pathology; D002417:Cattle; D002921:Cicatrix / Q000473:pathology; D003432:Cross-Linking Reagents / Q000737:chemistry; D005612:Freeze Drying; D005976:Glutaral / Q000737:chemistry; D006097:Granulation Tissue / Q000473:pathology; D015745:Granuloma, Foreign-Body / Q000473:pathology; D006350:Heart Valve Prosthesis; D008297:Male; D008422:Materials Testing; D010496:Pericardium / Q000473:pathology / Q000637:transplantation*; D011474:Prosthesis Design; D051381:Rats; D017207:Rats, Sprague-Dawley; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery*; D013997:Time Factors; D016707:Tissue Fixation; D014021:Tissue Preservation / Q000379:methods; D014183:Transplantation, Heterologous
D003432:Cross-Linking Reagents; D005976:Glutaral