Coimmunoprecipitation and proteomic analyses.
Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.
Falsone
S Fabio
SF
Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria.
Gesslbauer
Bernd
B
Kungl
Andreas J
AJ
D015153:Blotting, Western; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D036103:Nanotechnology; D040901:Proteomics