Physicochemical properties of fluorescent probes: experimental and computational determination of the overlapping pKa values of carboxyfluorescein.
The overlapping pKa values of carboxyfluorescein have been measured by applying a UV-vis spectroscopic method that uses absorbance diagrams and chemometric analysis. Quantum chemical calculations have also been performed in order to analyze the experimental data.
Aschi
Massimiliano
M
Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università degli Studi de L'Aquila, Via Vetoio (Coppito II), 67010 Coppito, L'Aquila, Italy.
D'Archivio
Angelo A
AA
Fontana
Antonella
A
Formiglio
Alessandra
A
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003198:Computer Simulation; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D015394:Molecular Structure; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
D005452:Fluoresceins; D005456:Fluorescent Dyes; D007477:Ions; D014867:Water; C024098:6-carboxyfluorescein