title: [Sentinel lymph node mapping of the stomach using fluorescent magnetic nanoparticles in rabbits].
abstract: OBJECTIVE
  Sentinel lymph node (SLN) mapping of the stomach cancer using available techniques is limited by unpredictable lymphatic drainage patterns and skip metastasis. The aim of this study was to test the feasibility of gastric SLN mapping using fluorescent magnetic nanoparticles (FMNP) of uniform nano-size.
  
  
  METHODS
  Biocompatible silica-overcoated magnetic nanoparticles containing rhodamine B isothiocyanate (RITC) within a silica shell of controllable thickness with 60 nm thickness were used as model nanomaterials. Gastric lymphatic mapping was performed by injecting 100 microL of either FMNP or isosulafan blue subserosally. Gastric injections (n=7) were made into the body, approximately 5 cm from the lesser curvature of rabbits. Sentinel lymph nodes were visualized using fluorescent nanoparticle detection system.
  
  
  RESULTS
  In 7 rabbits, it was demonstrated that FMNP quickly and accurately detected sentinel lymph nodes. Injection into the stomach resulted in identification of a retrogastric lymph node. Histological analysis in all cases confirmed the presence of nodal tissue.
  
  
  CONCLUSIONS
  FMNP can be a potential alternative to existing tracers in the detection of SLN in this animal experiment.
authors: Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
authors: Jin Soo
authors: JS
authors: Kim
authors: Tae Jong
authors: TJ
authors: Yoon
authors: Hyung Keun
authors: HK
authors: Kim
authors: Sung Soo
authors: SS
authors: Kim
authors: Hiun Suk
authors: HS
authors: Chae
authors: Myung Gyu
authors: MG
authors: Choi
authors: Yong Jin
authors: YJ
authors: Kim
authors: Gyu Chul
authors: GC
authors: Yi
authors: Young Seok
authors: YS
authors: Cho
mesh_terms: D000818:Animals; D004396:Coloring Agents; D005260:Female; D005456:Fluorescent Dyes; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D023421:Models, Animal; D053758:Nanoparticles; D011817:Rabbits; D012235:Rhodamines; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013270:Stomach / Q000473:pathology*; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; D012394:Rosaniline Dyes; C025484:iso-sulfan blue; C027755:rhodamine isothiocyanate