title: Calcium dynamics in the peroxisomal lumen of living cells.
abstract: We here describe the generation of novel, green fluorescent protein-based Ca(2+) indicators targeted to the peroxisome lumen. We show that (i) the Ca(2+) concentration of peroxisomes in living cells at rest is similar to that of the cytosol; (ii) increases in cytosolic Ca(2+) concentration (elicited by either Ca(2+) mobilization from stores or Ca(2+) influx through plasma membrane Ca(2+) channels) are followed by a slow rise in intraperoxisomal [Ca(2+)]; (iii) Ca(2+) influx into peroxisomes is driven neither by an ATP-dependent pump nor by membrane potential nor by a H(+)(Na(+)) gradient. The peroxisomal membrane appears to play a low pass filter role, preventing the organelle from taking up shortlasting cytosolic Ca(2+) transients but allowing equilibration of the peroxisomal luminal [Ca(2+)] with that of the cytosol during prolonged Ca(2+) increases. Thus, peroxisomes appear to be an additional cytosolic Ca(2+) buffer, but their influx and efflux mechanisms are unlike those of any other cellular organelle.
authors: Department of Biomedical Sciences and Consiglio Nazionale delle Ricerche Institute of Neuroscience, University of Padua and Venetian Institute of Molecular Medicine, Padua, Italy.
authors: Ilaria
authors: I
authors: Drago
authors: Marta
authors: M
authors: Giacomello
authors: Paola
authors: P
authors: Pizzo
authors: Tullio
authors: T
authors: Pozzan
mesh_terms: D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002138:Calibration; D002460:Cell Line; D002470:Cell Survival; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020675:Peroxisomes / Q000378:metabolism*; D052683:Somatotrophs
chemical_list: D002118:Calcium