[Development of recombinant scFv-antibodies against diphtheria toxin using phage display system].
Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 x 10(7) M(-1) was selected. scFv preserved activity on storage in the course of 8 months.
Oleĭnik
E S
ES
Kaberniuk
A A
AA
Burkaleva
D A
DA
Romaniuk
S I
SI
Kolibo
D V
DV
Shepeliakovskaia
A O
AO
Laman
A G
AG
Komisarenko
S V
SV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004165:Diphtheria / Q000175:diagnosis / Q000276:immunology; D004167:Diphtheria Toxin / Q000276:immunology*; D004355:Drug Stability; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011993:Recombinant Fusion Proteins