OX40 (CD134) expression in sentinel lymph nodes correlates with prognostic features of primary melanomas.
BACKGROUND
The expression of OX40 (CD134) on activated CD4+ T cells has been associated with favorable cancer patient outcomes. Because of recent reports that sentinel lymph nodes (SLNs) may represent an immunosuppressive environment, we investigated the expression of OX40 in SLNs from patients with primary cutaneous melanoma.


METHODS
Samples of peripheral blood lymphocytes and a section of 71 SLNs from 53 patients with clinically node negative melanoma were purified for CD4+ T cells, stained for OX40, and analyzed by flow cytometry.


RESULTS
The mean percentage of OX40 on CD4 T cells in the SLNs versus peripheral blood lymphocytes was related indirectly to the T stage of the primary tumor and was decreased in ulcerated primary tumors and positive sentinel nodes.


CONCLUSIONS
The expression of OX40 on CD4+ T cells in SLNs draining primary melanomas decreased with more advanced tumor features (higher T stage, ulceration) and nodal involvement, suggesting that such tumors may have an immunosuppressive effect on the SLN microenvironment.
Sarff
MaryClare
M
Earl A. Chiles Research Institute, Providence Portland Medical Center, Portland, OR, USA.
Edwards
David
D
Dhungel
Birat
B
Wegmann
Keith W
KW
Corless
Christopher
C
Weinberg
Andrew D
AD
Vetto
John T
JT
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007167:Immunotherapy; D008198:Lymph Nodes / Q000378:metabolism*; D008545:Melanoma / Q000276:immunology / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D009367:Neoplasm Staging; D053262:Receptors, OX40 / Q000378:metabolism*; D012878:Skin Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D018709:Statistics, Nonparametric; D050378:T-Lymphocytes, Regulatory
D053262:Receptors, OX40