title: A simple silica-based method for metagenomic DNA extraction from soil and sediments.
abstract: A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.
authors: Universidad Autónoma de Yucatán, Ciudad Industrial, Mérida, Yucatán, Mexico. r.rojas@uady.mx
authors: R
authors: R
authors: Rojas-Herrera
authors: J
authors: J
authors: Narváez-Zapata
authors: M
authors: M
authors: Zamudio-Maya
authors: M E
authors: ME
authors: Mena-Martínez
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D023281:Genomics; D019015:Geologic Sediments / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*; D012987:Soil / Q000032:analysis*
chemical_list: D017931:DNA Primers; D012987:Soil; D012822:Silicon Dioxide; D004247:DNA