SHORT HYPOCOTYL IN WHITE LIGHT1, a serine-arginine-aspartate-rich protein in Arabidopsis, acts as a negative regulator of photomorphogenic growth.
Light is an important factor for plant growth and development. We have identified and functionally characterized a regulatory gene SHORT HYPOCOTYL IN WHITE LIGHT1 (SHW1) involved in Arabidopsis (Arabidopsis thaliana) seedling development. SHW1 encodes a unique serine-arginine-aspartate-rich protein, which is constitutively localized in the nucleus of hypocotyl cells. Transgenic analyses have revealed that the expression of SHW1 is developmentally regulated and is closely associated with the photosynthetically active tissues. Genetic and molecular analyses suggest that SHW1 acts as a negative regulator of light-mediated inhibition of hypocotyl elongation, however, plays a positive regulatory role in light-regulated gene expression. The shw1 mutants also display shorter hypocotyl in dark, and analyses of shw1 cop1 double mutants reveal that SHW1 acts nonredundantly with COP1 to control hypocotyl elongation in the darkness. Taken together, this study provides evidences that SHW1 is a regulatory protein that is functionally interrelated to COP1 and plays dual but opposite regulatory roles in photomorphogenesis.
Bhatia
Shikha
S
National Institute for Plant Genome Research, New Delhi 110067, India.
Gangappa
Sreeramaiah N
SN
Kushwaha
Ritu
R
Kundu
Snehangshu
S
Chattopadhyay
Sudip
S
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000254:growth & development* / Q000502:physiology / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002466:Cell Nucleolus / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D029863:Cicer / Q000235:genetics*; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000254:growth & development* / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics / Q000528:radiation effects; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
D029681:Arabidopsis Proteins; D010940:Plant Proteins; C530965:SHW1 protein, Arabidopsis; D002734:Chlorophyll; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases