Immunoproteomic assay of surface proteins of Streptococcus suis serotype 9.
Streptococcus suis is an important swine pathogen responsible for a diverse group of diseases. Studies on vaccines have focused on S. suis serotype 2 strains, which are the most invasive isolates worldwide. However, in China S. suis serotype 9 (SS9) is also a prevalent serotype, which is frequently isolated from diseased pigs. Little is known about immunogenic proteins for SS9. Therefore, an immunoproteomic-based approach was developed to identify immunogenic proteins of SS9. Cell wall proteins extracted from SS9 strain GZ0565 isolated from a diseased pig with meningitis were screened by two-dimensional Western blotting using anti-SS9 sera pooled from specific pathogen-free mice. Protein spots were excised from preparative gels and identified by matrix-assisted laser desorption ionization time-of-flight MS (MALDI-TOF-MS) or MALDI-TOF-TOF-MS, which led to the identification of eight immunogenic proteins (arginine deiminase, extracellular solute-binding protein, translation elongation factor Ts, neprilysin, peptide ATP-binding cassette transporter peptide-binding protein, pyruvate kinase, phosphate acetyltransferase, and fructose-bisphosphate aldolase). These immunogenic proteins, which are encoded by genes that are reasonably conserved among SS9 strains, could be developed as vaccine candidates.
Wu
Zongfu
Z
Key Lab Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.
Zhang
Wei
W
Lu
Chengping
C
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D016133:Polymerase Chain Reaction / Q000662:veterinary; D040901:Proteomics / Q000379:methods; D013047:Specific Pathogen-Free Organisms; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D017011:Streptococcus suis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins