title: Suppression of ClC-3 channel expression reduces migration of nasopharyngeal carcinoma cells.
abstract: Recent studies suggest that chloride (Cl-) channels regulate tumor cell migration. In this report, we have used antisense oligonucleotides specific for ClC-3, the most likely molecular candidate for the volume-activated Cl- channel, to investigate the role of ClC-3 in the migration of nasopharyngeal carcinoma cells (CNE-2Z) in vitro. We found that suppression of ClC-3 expression inhibited the migration of CNE-2Z cells in a concentration-dependent manner. Whole-cell patch-clamp recordings and image analysis further demonstrated that ClC-3 suppression inhibited the volume-activated Cl- current (I(Cl,vol)) and regulatory volume decrease (RVD) of CNE-2Z cells. The expression of ClC-3 positively correlated with cell migration, I(Cl,vol) and RVD. These results strongly suggest that ClC-3 is a component or regulator of the volume-activated Cl- channel. ClC-3 may regulate CNE-2Z cell migration by modulating cell volume. ClC-3 may be a new target for cancer therapies.
authors: Institute of Basic Medical Sciences and Department of Biology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors: Jianwen
authors: J
authors: Mao
authors: Lixin
authors: L
authors: Chen
authors: Bin
authors: B
authors: Xu
authors: Lijing
authors: L
authors: Wang
authors: Hongzhi
authors: H
authors: Li
authors: Jiao
authors: J
authors: Guo
authors: Weidong
authors: W
authors: Li
authors: Sihuai
authors: S
authors: Nie
authors: Tim J C
authors: TJ
authors: Jacob
authors: Liwei
authors: L
authors: Wang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D018118:Chloride Channels / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D002712:Chlorides / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D020128:Inhibitory Concentration 50; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D014162:Transfection
chemical_list: D018118:Chloride Channels; D002712:Chlorides; C115376:ClC-3 channel; D016376:Oligonucleotides, Antisense