title: [Search of streptomycin-resistant bacteria in creek water and application of MALDI-TOF MS to grouping of the isolated bacteria].
abstract: A search of streptomycin-resistant bacteria was carried out using ten creek water samples collected in Saga prefecture by spreading the sample water on an R2A medium containing 10 microg/ml of streptomycin. It was clarified that such streptomycin-resistant bacteria as Bacillus, Novosphigobium, Sphingopyxis and Oceanobacillus were distributed in the creek water. Further, 60% of the isolates didn't form an inhibitory zone by the application of 700 microg/ml streptomycin solution in the cup method assay. Further, the effectiveness of the MALDI-TOF MS analysis for the grouping of the isolates was examined. The discriminating ability of MALDI-TOF MS analysis was higher than that of RFLP analysis and it was almost equal to that of sequence analysis using 16S rDNA. Considering the high-throughput ability of the MALDI-TOF MS instrument, MALDI-TOF mass spectral identification of bacteria will be a powerful method in the construction of a MALDI-TOF mass spectra database.
authors: Graduate School of Engineering, Fukuoka Institute of Technology. Higashi-ku, Fukuoka 811-0295, Japan.
authors: Yayoi
authors: Y
authors: Ichiki
authors: Tomohiro
authors: T
authors: Aoki
authors: Yasuto
authors: Y
authors: Takashima
authors: Hiroto
authors: H
authors: Tamura
authors: Kanae
authors: K
authors: Teramoto
authors: Hiroaki
authors: H
authors: Sato
authors: Keiichi
authors: K
authors: Arashidani
authors: Hiromichi
authors: H
authors: Yoshikawa
mesh_terms: D016573:Agrochemicals / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D012150:Polymorphism, Restriction Fragment Length; D045483:Rivers / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D013307:Streptomycin / Q000494:pharmacology*
chemical_list: D016573:Agrochemicals; D000900:Anti-Bacterial Agents; D013307:Streptomycin