Evaluation of recombinant fructose-1,6-bisphosphate aldolase ELISA test for the diagnosis of Schistosoma japonicum in water buffaloes.
Fructose-1,6-bisphosphate aldolase (FBPA) is an ubiquitous enzyme essential for glycolysis, gluconeogenesis and the Calvin cycle. It has been demonstrated to induce immune responses and to be useful in the immunodiagnosis of malaria. In this study, FBPA was cloned from the adult worms of Schistosoma japonicum and tested as an antigen for the diagnosis of S. japonicum infection in water buffaloes. Enzyme-linked immunosorbent assay (ELISA) was performed on the sera from 32 infected water buffaloes and 20 negative controls using the recombinant FBPA protein or soluble worm antigen preparation (SWAP) as an antigen. The OD cut-off values were determined to be 0.57 with 100% specificity and 100% sensitivity for the FBPA ELISA and 1.13 with 93.8% specificity and 95.0% sensitivity for the SWAP ELISA. These findings indicate that the recombinant FBPA of S. japonicum should be an useful diagnostic tool for the detection of antibodies against S. japonicum.
Peng
Shih-Yi
SY
Institute of Tropical Medicine, National Yang-Ming University, Taipei 112, Taiwan.
Lee
Kin-Mu
KM
Tsaihong
John Chin
JC
Cheng
Po-Ching
PC
Fan
Ping-Chin
PC
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D001483:Base Sequence; D002020:Buffaloes / Q000469:parasitology*; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D005784:Gene Amplification; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000175:diagnosis / Q000662:veterinary*
D000909:Antibodies, Helminth; D018076:DNA, Complementary; D011994:Recombinant Proteins; D005634:Fructose-Bisphosphate Aldolase