[Sentinel lymph node mapping of the stomach using fluorescent magnetic nanoparticles in rabbits].
OBJECTIVE
Sentinel lymph node (SLN) mapping of the stomach cancer using available techniques is limited by unpredictable lymphatic drainage patterns and skip metastasis. The aim of this study was to test the feasibility of gastric SLN mapping using fluorescent magnetic nanoparticles (FMNP) of uniform nano-size.


METHODS
Biocompatible silica-overcoated magnetic nanoparticles containing rhodamine B isothiocyanate (RITC) within a silica shell of controllable thickness with 60 nm thickness were used as model nanomaterials. Gastric lymphatic mapping was performed by injecting 100 microL of either FMNP or isosulafan blue subserosally. Gastric injections (n=7) were made into the body, approximately 5 cm from the lesser curvature of rabbits. Sentinel lymph nodes were visualized using fluorescent nanoparticle detection system.


RESULTS
In 7 rabbits, it was demonstrated that FMNP quickly and accurately detected sentinel lymph nodes. Injection into the stomach resulted in identification of a retrogastric lymph node. Histological analysis in all cases confirmed the presence of nodal tissue.


CONCLUSIONS
FMNP can be a potential alternative to existing tracers in the detection of SLN in this animal experiment.
Kim
Jin Soo
JS
Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
Yoon
Tae Jong
TJ
Kim
Hyung Keun
HK
Kim
Sung Soo
SS
Chae
Hiun Suk
HS
Choi
Myung Gyu
MG
Kim
Yong Jin
YJ
Yi
Gyu Chul
GC
Cho
Young Seok
YS
D000818:Animals; D004396:Coloring Agents; D005260:Female; D005456:Fluorescent Dyes; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D023421:Models, Animal; D053758:Nanoparticles; D011817:Rabbits; D012235:Rhodamines; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*; D013270:Stomach / Q000473:pathology*; D013997:Time Factors
D004396:Coloring Agents; D005456:Fluorescent Dyes; D012235:Rhodamines; D012394:Rosaniline Dyes; C025484:iso-sulfan blue; C027755:rhodamine isothiocyanate