Regulation of RhoBTB2 by the Cul3 ubiquitin ligase complex.
The recently identified RhoBTB family is a member of the Rho GTPase family. One family member, RhoBTB2, has been implicated as a tumor suppressor in lung and breast cancer. Studies have shown that RhoBTB2 binds to the ubiquitin ligase scaffold Cul3 and that Cul3 regulates RhoBTB2 protein levels by ubiquitinating RhoBTB2 directly, leading to its degradation by the proteasome. This chapter details the cell biological and biochemical methods for analyzing the regulation of RhoBTB2 by Cul3.
Wilkins
Andrew
A
Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
Carpenter
Christopher L
CL
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002460:Cell Line; D044842:Cullin Proteins / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000276:immunology / Q000502:physiology*; D006041:Goats; D006367:HeLa Cells; D006801:Humans; D046912:Multiprotein Complexes / Q000302:isolation & purification; D011817:Rabbits; D025521:Tumor Suppressor Proteins / Q000276:immunology / Q000502:physiology*; D054875:Ubiquitination
D000906:Antibodies; C503645:CUL3 protein, human; D044842:Cullin Proteins; D046912:Multiprotein Complexes; C497336:RHOBTB2 protein, human; D025521:Tumor Suppressor Proteins; D019204:GTP-Binding Proteins