Expression of avian prickle genes during early development and organogenesis.
Chicken homologues of prickle-1 (pk-1) and prickle-2 (pk-2) were isolated to gain insight into the extent of planar cell polarity signaling during avian embryogenesis. Bioinformatics analyses demonstrated homology and showed that pk-1 and pk-2 exhibited conserved synteny with ADAMTS20 and ADAMTS9, GON-related zinc metalloproteases. Expression of pk-1 and pk-2 was established during embryogenesis and early organogenesis, using in situ hybridization and sections of chicken embryos. At early stages, pk-1 was expressed in Hensen's node, primitive streak, ventral neural tube, and foregut. In older embryos, pk-1 transcripts were detected in dorsolateral epithelial somites, dorsomedial lip of dermomyotomes, and differentiating myotomes. Furthermore, pk-1 expression was seen in lateral body folds, limb buds, and ventral metencephalon. pk-2 was expressed in Hensen's node and neural ectoderm at early stages. In older embryos, pk-2 expression was restricted to ventromedial epithelial somites, except in the most recently formed somite pair, and limb bud mesenchyme.
Cooper
Oliver
O
School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
Sweetman
Dylan
D
Wagstaff
Laura
L
Münsterberg
Andrea
A
D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo / Q000033:anatomy & histology / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D060588:LIM Domain Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000502:physiology*; D010802:Phylogeny; D016415:Sequence Alignment; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
D060588:LIM Domain Proteins; D008565:Membrane Proteins; C515173:PRICKLE1 protein, human; C518732:Prickle2 protein, mouse; D025521:Tumor Suppressor Proteins