Perfluorocarbons: new tool for islets preservation in vitro.
Pancreatic islet transplantations to treat type 1 diabetes often fail to function because of hypoxia. Perfluorocarbons (PFCs) exhibit a high oxygen solubility coefficient and maintain high oxygen partial pressure for extended times. They also serve as oxygen "reservoirs" for harvested organs in pancreas organ transplantation. Previous studies have shown the PFCs display antiadhesive effects on beta cells. The aim of this study was to evaluate the effects of PFC on islet viability and functionality and on extracellular matrix (ECM) disruption of islets via inhibition of adhesion. Primary cultures of rat islets were incubated for 24 hours in the presence or absence of 3.5% (weight/volume) PFCs in culture media. We studied viability (FDA/PI), stimulation index linked to insulin secretion (ELISA), and expression of insulin and laminin messenger RNAs (mRNAs). Immunostaining was performed on insulin and laminin. Islet viability was similar in the presence or absence of PFCs (about 80%). Stimulation index showed preservation of islet functionality in the presence of PFC (4.9 +/- 0.7) as compared with controls (2.8 +/- 0.5). Moreover, laminin mRNA expression was lower compared with controls (55% of PFC incubated vs control islets). Immunohistochemistry studies showed preservation of ECM inside the islets in the presence of PFCs versus controls at 24 hours after islet isolation. In conclusion, PFCs preserved islet viability and functionality and prevented ECM disruption. PFCs may represent a new tool for islet preservation in vitro.
Maillard
E
E
Centre Européen d'Etude du Diabète, Strasbourg, France.
Sanchez-Dominguez
M
M
Kleiss
C
C
Langlois
A
A
Sencier
M C
MC
Vodouhe
C
C
Beitigier
W
W
Krafft
M P
MP
Pinget
M
M
Belcourt
A
A
Sigrist
S
S
D000199:Actins / Q000235:genetics; D000818:Animals; D005466:Fluorocarbons / Q000494:pharmacology*; D007150:Immunohistochemistry; D007328:Insulin / Q000235:genetics; D007515:Islets of Langerhans / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D007797:Laminin / Q000235:genetics; D009929:Organ Size; D010179:Pancreas / Q000033:anatomy & histology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014021:Tissue Preservation / Q000379:methods*
D000199:Actins; D005466:Fluorocarbons; D007328:Insulin; D007797:Laminin; D012333:RNA, Messenger