title: More about the role of 2,6-dichlorophenol in tick courtship: identification and olfactometer bioassay in Amblyomma cajennense and Rhipicephalus sanguineus.
abstract: This study aimed to identify 2,6-dichlorophenol (2,6-DCP) in Amblyomma cajennense and to evaluate its role in A. cajennense and Rhipicephalus sanguineus courtship. Hexanic extract from attractive females was purified by solid phase extraction and the phenol was identified by the single ion monitoring method using GC/MS. In an olfactometer, the responses of A. cajennense and R. sanguineus males to females, control rubber septa or rubber septa impregnated with 2,6-DCP at 50, 500, and 5000 ng, respectively, were studied. 2,6-DCP was identified in A. cajennense extract and the males oriented themselves toward the concentration of 500 ng. These septa and the females were recognized as copula partners. The septa treated with 2,6-DCP did not attract and were not even recognized by the R. sanguineus males, whereas the females were recognized. Due to the presence of 2,6-DCP in A. cajennense and the results of biological bioassays, it was concluded that this compound acts as an attractant and mounting sex pheromone in this tick, but it does not play any role in R. sanguineus courtship.
authors: Escola de Veterinária, Universidade Federal de Goiás.
authors: Carla Cristina Braz
authors: CC
authors: Louly
authors: Diana da Nóbrega
authors: Dda N
authors: Silveira
authors: Sara Fernandes
authors: SF
authors: Soares
authors: Pedro Henrique
authors: PH
authors: Ferri
authors: Ariane Carla Campos de
authors: AC
authors: Melo
authors: Lígia Miranda Ferreira
authors: LM
authors: Borges
mesh_terms: D000818:Animals; D001681:Biological Assay; D002733:Chlorophenols / Q000302:isolation & purification / Q000494:pharmacology*; D003380:Courtship; D004305:Dose-Response Relationship, Drug; D005260:Female; D026863:Ixodidae / Q000187:drug effects* / Q000502:physiology; D008297:Male; D048508:Rhipicephalus sanguineus / Q000187:drug effects / Q000502:physiology; D012724:Sex Attractants / Q000302:isolation & purification / Q000494:pharmacology* / Q000502:physiology; D012726:Sexual Behavior, Animal / Q000187:drug effects* / Q000502:physiology
chemical_list: D002733:Chlorophenols; D012724:Sex Attractants; C032729:2,6-dichlorophenol