title: Isolation of human adipose-derived stem cells from biopsies and liposuction specimens.
abstract: Adipose tissue has proven to serve as an abundant, accessible, and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe a detailed method for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens and a small scale procedure suitable for processing adipose tissue biopsy specimens of < 0.5 g. Although we have focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.
authors: Pennington Biomedical Research Center, Baton Rouge, LA, USA.
authors: Severine G
authors: SG
authors: Dubois
authors: Elizabeth Z
authors: EZ
authors: Floyd
authors: Sanjin
authors: S
authors: Zvonic
authors: Gail
authors: G
authors: Kilroy
authors: Xiying
authors: X
authors: Wu
authors: Stacy
authors: S
authors: Carling
authors: Yuan Di C
authors: YD
authors: Halvorsen
authors: Eric
authors: E
authors: Ravussin
authors: Jeffrey M
authors: JM
authors: Gimble
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000473:pathology; D001706:Biopsy; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D015187:Lipectomy; D059630:Mesenchymal Stem Cells / Q000166:cytology; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering