On the sulfation and methylation of catecholestrogens in human mammary epithelial cells and breast cancer cells.
Prolonged exposure to high level of estrogen is a known risk factor for breast carcinogenesis. In cells, estrogens, in particular estrone (E1) and 17 beta-estradiol (E2), can be converted to catecholestrogens (CEs) which may be oxidized to form CE-semiquinones and CE-quinones that are capable of binding to DNA to induce mutations, followed by carcinogenesis. Whether the body is equipped with protective mechanisms against potentially harmful CEs, therefore, is an important issue. The present study was designed to examine the role of sulfation in the metabolism of CEs. MCF-7 breast cancer cells and MCF 10A human mammary epithelial cells were metabolically labeled with [35S]sulfate in the presence of individual CEs. Analysis of the labeling media showed the generation and release of exclusively [35S]sulfated 2-methoxy-E1 or [35S]sulfated 2- or 4-methoxy-E2 by cells labeled in the presence of 2-OH-E1 or 2- or 4-OH-E2. Whereas both [35S]sulfated 4-methoxy-E1 and [35S]sulfated 4-OH-E1 were detected in the labeling media of cells labeled in the presence of 4-OH-E1. These results indicated a concerted action of catechol-O-methyltransferase (COMT) and the cytosolic sulfotransferase (SULT) enzyme(s) in the metabolism of CEs. Enzymatic assays revealed that, five (SULT1A1, SULT1A2, SULT1A3, SULT1C4, and SULT1E1) of eleven known human SULTs tested could use CEs and methoxyestrogens (MEs) as substrates, with SULT1E1 displaying the strongest sulfating activity.
Hui
Ying
Y
Department of Pharmacology, College of Pharmacy, The University of Toledo, Toledo, OH 43606, USA.
Yasuda
Shin
S
Liu
Ming-Yih
MY
Wu
Yi-Yong
YY
Liu
Ming-Cheh
MC
D001940:Breast / Q000166:cytology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism*; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D002396:Catechols / Q000737:chemistry*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D004967:Estrogens / Q000737:chemistry*; D005260:Female; D006801:Humans; D008745:Methylation; D011994:Recombinant Proteins / Q000737:chemistry; D013431:Sulfates / Q000378:metabolism*; D015238:Sulfotransferases / Q000378:metabolism; D013462:Sulfur Radioisotopes / Q000737:chemistry; D014334:Tropolone / Q000494:pharmacology
D002396:Catechols; D004967:Estrogens; D011994:Recombinant Proteins; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014334:Tropolone; D002394:Catechol O-Methyltransferase; D015238:Sulfotransferases