title: [Therapeutic angiogenesis induced by hepatocyte growth factor directed by ultrasound-targeted microbubble destruction].
abstract: OBJECTIVE
  To explore the feasibility of therapeutic angiogenesis in myocardial infarction induced by hepatocyte growth factor (HGF) mediated by ultrasound-targeted microbubble destruction.
  
  
  METHODS
  Forty Wistar rats were divided into 4 groups after the models of myocardial infarction were established: HGF + ultrasound + microbubble (HGF + US/MB) groups, HGF and ultrasound (HGF + US) group, HGF and microbubble (HGF + MB) group, and surgery alone (SA) group. Ultrasound-targeted destruction microbubble loaded with HGF gene with ECG trigger was performed in HGF + US group. Microbubble loaded with HGF gene was infused intravenously in HGF + MB group, and normal saline were infused in SA group. All rats were killed 14 days after transfection. The CD34 expression was detected by immunohistochemistry (IHC), and microvessel density (MVD) was counted in high power field. The HGF expression on myocardium was detected by ELISA, and the correlation between the contents of HGF and MVD in myocardium was analyzed.
  
  
  RESULTS
  IHC results showed that CD34 expressions, shown as brown granules, were located on the membrane and endochylema of vascular endothelial cells. The MVD in HGF + US/MB group [ (266.9 +/- 39.8) /HPF] were highest among all the groups. The contents of HGF in myocardium were highest in HGF + US/MB group [(5.54 +/- 0.81) ng/g], and the contents of HGF in anterior wall were significantly higher than those in posterior wall (P < 0.05); the difference was also significant when compared with others groups (P < 0.01). The correlation analysis showed the contents of HGF was positively correlated with MVD in myocardium.
  
  
  CONCLUSIONS
  Ultrasound-targeted microbubble destruction can effectively deliver HGF into the infracted myocardium and facilitate angiogenesis, which provides a novel way in the gene therapy of myocardial infarction.
authors: Institute of Ultrasound Imaging, Chongqing University of Medical Sciences, Chongqing 400010, China. wzg62942443@163.com
authors: Zhi-Gang
authors: ZG
authors: Wang
authors: Xing-Sheng
authors: XS
authors: Li
authors: Xue-Lin
authors: XL
authors: Li
authors: Qiao-Ying
authors: QY
authors: Yuan
authors: Jian-Li
authors: JL
authors: Ren
authors: Hai-Tao
authors: HT
authors: Ran
authors: Pan
authors: P
authors: Li
authors: Chun-Jiang
authors: CJ
authors: Yang
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems; D017228:Hepatocyte Growth Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D045423:Microbubbles; D055806:Microvessels / Q000187:drug effects / Q000503:physiopathology; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014465:Ultrasonics; D014463:Ultrasonography
chemical_list: D017228:Hepatocyte Growth Factor