Cytochrome-b variation in Apis mellifera samples and its association with COI-COII patterns.
Five Mbo I (Mbo-A, Mbo-M, Mbo-C(1), Mbo-C(2) and Mbo-C(3)) and Hinf I (Hinf-1 to Hinf-5) patterns were observed in Apis mellifera samples after restriction of a 485 bp fragment of the mitochondrial cytochrome-b (cyt-b) gene. Associating the cyt-b Restriction fragment length polymorphism (RFLP) pattern of each sample to its respective previously established COI-COII (Dra I sites) pattern, five restriction patterns (Mbo-C(1), Mbo-C(2), Mbo-C(3), Hinf-1 and Hinf-4) were observed in samples of maternal origin associated to the evolutionary branch C. No deletions or insertions were observed and the nucleotide substitution rate was estimated at 5.4%. Higher nucleotide diversity was observed among the branch C-haplotypes when compared with A and M lineages. Further studies are needed to confirm if the cyt-b + COI-COII haplotypes help to assign certain phylogeographic patterns to the branch C and to clarify phylogenetic relationships among A. mellifera subspecies.
Ferreira
Kátia M
KM
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Paulo, Rodovia Washington Luís km 235, São Carlos, São Paulo, 13565.905, Brazil.
Lino e Silva
Otávio
O
Arias
Maria C
MC
Del Lama
Marco A
MA
D000818:Animals; D001516:Bees / Q000201:enzymology* / Q000235:genetics*; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation; D006239:Haplotypes; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D045303:Cytochromes b; D003576:Electron Transport Complex IV