title: Prevalence of genes encoding extracellular virulence factors among meticillin-resistant Staphylococcus aureus isolates from the University Hospital, Olomouc, Czech Republic.
abstract: A rather fast and complicated progression of an infection caused by some strains of Staphylococcus aureus could be associated with the expression and co-action of virulence factor complexes in these strains. This study screened the antibiotic susceptibility and prevalence of virulence markers in isolates of meticillin-resistant S. aureus (MRSA) obtained from patients hospitalized at the University Hospital in Olomouc, Czech Republic. A total of 100 isolates was screened for 13 genes encoding extracellular virulence determinants (tst, pvl, eta, etb, sea, seb, sec, sed, see, seg, seh, sei and sej) and for their distribution in sample types. Eighty-nine isolates were positive for at least one of the genes. Genes for etb, pvl, see and seh were not detected in any of the MRSA isolates. No statistically significant differences in the occurrence of the determinants studied among sample types were found.
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: P
authors: P
authors: Sauer
authors: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: J
authors: J
authors: Síla
authors: Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: T
authors: T
authors: Štosová
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: R
authors: R
authors: Večeřová
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: P
authors: P
authors: Hejnar
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: I
authors: I
authors: Vágnerová
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: M
authors: M
authors: Kolář
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: V
authors: V
authors: Raclavský
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: J
authors: J
authors: Petrželová
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: Y
authors: Y
authors: Lovečková
authors: University Hospital, Olomouc, Czech Republic.|Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic.
authors: D
authors: D
authors: Koukalová
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D018153:Czech Republic / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D006785:Hospitals, University; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D015995:Prevalence; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D037521:Virulence Factors