title: [Electron microscopic study of Burkholderia cepacia biofilms].
abstract: Using the methods of transmission electron microscopy, the structure of the biofilms formed by the bacterium Burkholderia cepacia (clinical isolate and mutants with an increased and decreased ability to produce biofilms) were investigated. The biofilms were obtained on a liquid nutrient medium or on an abiotic surface (polystyrene). It has been demonstrated that the cultures of the studied strains differ in some morphological and functional characteristics. In biofilms, changes in the size and submicroscopic organization of all the components of bacterial cells occur. Staining biofilms with ruthenium red revealed the presence of exopolysaccharides in the intercellular space. The differences in the ultrastructure of bacterial films formed on nutrient medium and abiotic surfaces were demonstrated.
authors: T A
authors: TA
authors: Smirnova
authors: L V
authors: LV
authors: Didenko
authors: A L
authors: AL
authors: Andreev
authors: N V
authors: NV
authors: Aklekseeva
authors: T V
authors: TV
authors: Stepanova
authors: Iu M
authors: IuM
authors: Romanova
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D019121:Burkholderia Infections / Q000382:microbiology; D016956:Burkholderia cepacia / Q000502:physiology* / Q000648:ultrastructure; D003470:Culture Media; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D011137:Polystyrenes
chemical_list: D003470:Culture Media; D011137:Polystyrenes