Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma.
Diethylnitrosamine (DEN) is a known carcinogen that can alkylate DNA molecules. In rats, DEN-induced hepatocellular carcinoma (HCC) model is well established. In this study, we used a two-dimensional differential gel electrophoresis (2D-DIGE) system and liquid chromatography/mass spectrometry/mass spectrometry to identify the differential expression protein profiles between the DEN-induced HCC and healthy liver cells. Western blotting and semiquantitative RT-PCR were used to further confirm the results. Seventeen differentially expressed spots were identified in DEN-induced HCC cells. Among all, the most prominent upregulated proteins include the members of the glutathione S-transferase super family, aldo-keto reductase superfamily and proteins involved in the response to oxidative stress. Downregulation was observed in 2 proteins that were known to contribute to hepatic dysfunction. This study provides the first comprehensive protein profiling of the DEN-induced HCC in rats. This model simulates the differential protein expression of human HCC and may be useful for further understanding the mechanism of HCC tumorigenesis.
Qi
Yanting
Y
Institute of Molecular and Chemical Biology, East China Normal University, Shanghai, China.
Chen
Xiaona
X
Chan
Chu-yan
CY
Li
Dan
D
Yuan
Chonggang
C
Yu
Fei
F
Lin
Marie C
MC
Yew
David T
DT
Kung
Hsiang-Fu
HF
Lai
Lihui
L
D000818:Animals; D001483:Base Sequence; D002273:Carcinogens / Q000633:toxicity*; D002853:Chromatography, Liquid; D017931:DNA Primers; D004052:Diethylnitrosamine / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005978:Glutathione / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry
D002273:Carcinogens; D017931:DNA Primers; D004052:Diethylnitrosamine; D005978:Glutathione