CD11b expression distinguishes sequential stages of peritoneal B-1 development.
Peritoneal cavity (PerC) B-1 cells have long been known to express CD11b, which is coexpressed with CD18 to form the Mac-1/CR3 complement receptor and adhesion molecule. However, although all PerC B-1 cells are commonly believed to express CD11b, we show here that nearly half of the cells in each of the PerC B-1 subsets (B-1a and B-1b) do not express this surface receptor. The CD11b(+) cells in each B-1 subset are larger and more granular and express higher levels of surface IgM than the CD11b(-) B-1 cells. In addition, the CD11b(+) B-1 cells initiate the formation of tightly associated doublets that are present at high frequency in adult PerC. Finally, and most importantly from a developmental standpoint, the CD11b(+) B-1 cells have a limited reconstitution capability: when sorted and transferred into congenic recipients, they reconstitute their own (CD11b(+)) B-1 subset but do not reconstitute the CD11b(-) B-1 subset. In contrast, CD11b(-) B-1 cells transferred under the same conditions efficiently replenish all components of the PerC B-1 population in appropriate proportions. During ontogeny, CD11b(-) B-1 cells appear before CD11b(+) B-1 cells. However, the clear phenotypic differences between the neonatal and adult CD11b B-1 subsets argue that although CD11b(-) B-1 give rise to CD11b(+) B-1 in both cases different forces may regulate this transition.
Ghosn
Eliver Eid Bou
EE
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5318, USA.
Yang
Yang
Y
Tung
James
J
Herzenberg
Leonard A
LA
Herzenberg
Leonore A
LA
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology*; D039481:CD11b Antigen / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology*; D010641:Phenotype
D039481:CD11b Antigen