[Effect of antioxidants on internucleosomal DNA fragmentation in thyroid tissue of patients with various thyroid pathology].
The in vitro effects of a-tocopherol and melatonin on the intensity of DNA internucleosomal fragmentation in tissue and neoplastic tissue of patients with thyroid pathology was studied. alpha-Tocopherol at a concentration of 10(-7) M and melatonin at a concentration of 10(-3) M were shown to inhibit internucleosomal DNA fragmentation in unchanged thyroid tissue, while in pathologically changed tissue and neoplastic tissue the effect of antioxidants was either absent, or those effects of preparations were prevailing, which were aimed at an increase in the intensity of internucleosomal DNA fragmentation. Some peculiarities of changes in the content of certain oligonucleosome fractions depending on the preparation, its dose, and thyroid disorder have been identified. The possible mechanisms of the effect of antioxidants on internucleosomal DNA fragmentation in normal thyroid tissue and in the presence of thyroid pathology are discussed.
Myshunina
T M
TM
Kalinichenko
O V
OV
Pil'kevych
L I
LI
Tron'ko
M D
MD
D000975:Antioxidants / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008550:Melatonin / Q000494:pharmacology; D009707:Nucleosomes / Q000187:drug effects / Q000473:pathology; D013959:Thyroid Diseases / Q000473:pathology*; D013961:Thyroid Gland / Q000187:drug effects / Q000473:pathology; D024502:alpha-Tocopherol / Q000494:pharmacology
D000975:Antioxidants; D009707:Nucleosomes; D024502:alpha-Tocopherol; D008550:Melatonin