Zwitterionic polymers exhibiting high resistance to nonspecific protein adsorption from human serum and plasma.
This study examined six different polymer and self-assembled monolayer (SAM) surface modifications for their interactions with human serum and plasma. It was demonstrated that zwitterionic polymer surfaces are viable alternatives to more traditional surfaces based on poly(ethylene glycol) (PEG) as nonfouling surfaces. All polymer surfaces were formed using atom transfer radical polymerization (ATRP) and they showed an increased resistance to nonspecific protein adsorption compared to SAMs. This improvement is due to an increase in the surface packing density of nonfouling groups on the surface, as well as a steric repulsion from the flexible polymer brush surfaces. The zwitterionic polymer surface based on carboxybetaine methacrylate (CBMA) also incorporates functional groups for protein immobilization in the nonfouling background, making it a strong candidate for many applications such as in diagnostics and drug delivery.
Ladd
Jon
J
Department of Chemical Engineering, University of Washington, Box 351750, Seattle, Washington 98195, USA.
Zhang
Zheng
Z
Chen
Shengfu
S
Hower
Jason C
JC
Jiang
Shaoyi
S
D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry*; D006801:Humans; D007477:Ions; D010949:Plasma; D011108:Polymers / Q000737:chemistry*; D011109:Polymethacrylic Acids; D044967:Serum
D001672:Biocompatible Materials; D001798:Blood Proteins; D007477:Ions; D011108:Polymers; D011109:Polymethacrylic Acids