Effect of some natural products either alone or in combination on gastritis induced in experimental rats.
Gastritis, an inflammatory state in gastric mucosa, can be induced experimentally in various ways. The present study considered the iodoacetamide model (Iodo). Omega-3 fatty acids (fish oil), black seed oil, and curcuminoids (natural products) in addition to omeprazole (synthetic proton-pump inhibitor) were tested. Supplementation of 0.1% iodoacetamide to drinking water of experimental rats for two consecutive weeks resulted in: (i) increased serum nitric oxide (NO) and gastrin, and decreased pepsinogen, (ii) depletion of gastric mucosal glutathione (GSH), and (iii) increased gastric mucosal lipid peroxidation (MDA), but failed to affect gastric mucosal myeloperoxidase (MPO) activity. Histological examination showed marked neutrophilic infiltration after 1 week of iodoacetamide administration and shedding of apical cell layer with pale edematous vacuolated gastric gland cells and thickening of muscularis mucosa after 2 weeks of iodoacetamide intake. Individual administration of omega-3 fatty acids 12 mg/kg, black seed oil 50 mg/kg, and curcuminoids 50 mg/kg body weight orally daily for 3 weeks decreased MDA, gastrin, and NO, and normalized mucosal GSH but failed to affect serum pepsinogen level. Combined administration of these natural products for 3 weeks normalized MPO activity, and other effects were nearly the same as with individual use. Omeprazole administration 30 mg/kg body weight orally daily for 3 weeks induced a similar response except for an observed increase in serum gastrin and pepsinogen levels.
Elseweidy
Mohamed M
MM
Biochemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt. mmelseweidy@yahoo.com
Younis
Nahla N
NN
Amin
Rawia S
RS
Abdallah
Fatma R
FR
Fathy
Azza M
AM
Yousif
Zeinab A
ZA
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D030024:Curcuma / Q000737:chemistry*; D004195:Disease Models, Animal; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology; D005755:Gastrins / Q000097:blood; D005756:Gastritis / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D007460:Iodoacetamide / Q000633:toxicity; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D009853:Omeprazole / Q000494:pharmacology; D020116:Pepsinogen A / Q000097:blood; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D015525:Fatty Acids, Omega-3; D005755:Gastrins; D009573:Nitrites; D010936:Plant Extracts; D010938:Plant Oils; D008315:Malondialdehyde; D020116:Pepsinogen A; D009195:Peroxidase; D009853:Omeprazole; D007460:Iodoacetamide