In vitro noncontact intravascular femtosecond laser surgery in models of branch retinal vein occlusion.
OBJECTIVE
To investigate intravenous femtosecond laser surgery in models of branch retinal vein occlusion.


METHODS
Non-amplified near infrared femtosecond laser was used to ablate polyamide sutures and human hairs inserted into the vascular lumina of porcine retinal veins in vitro. Specimens were subjected to multiphoton laser scanning microscopy and electron microscopy.


RESULTS
Regular laser cuts within sutures and hairs were detected with laser microscopy and electron microscopy. Neither laser microscopy nor histology revealed collateral damage of the vascular wall.


CONCLUSIONS
Non-amplified femtosecond lasers may allow precise atraumatic non-contact intravenous retinal surgery controlled by high-resolution imaging of the target.
Toropygin
Sergey
S
Department of Ophthalmology and Eye Hospital, University of Saarland, Homburg, Germany. doctorop@mail.ru
Krause
Matthias
M
Riemann
Iris
I
Hild
Matthias
M
Mestres
Pedro
P
Seitz
Berthold
B
Khurieva
Elena
E
Ruprecht
Klaus W
KW
Löw
Ursula
U
Gatzioufas
Zisis
Z
König
Karsten
K
D000818:Animals; D004195:Disease Models, Animal; D007259:Infrared Rays; D053685:Laser Therapy / Q000379:methods*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D010865:Pilot Projects; D012169:Retinal Vein / Q000601:surgery* / Q000648:ultrastructure; D012170:Retinal Vein Occlusion / Q000473:pathology / Q000601:surgery*; D013552:Swine