[Effect of arotinoid trometamol on the expression of genes in human keratinocytes].
OBJECTIVE
To investigate the gene expression pattern of human keratinocytes (KCs) after arotinoid trometamol (AT) treatment.


METHODS
Human keratinocytes (KCs) of the line HaCat were cultured. AT (10(-6) mol/L) was added in the culture fluid for 24 h. A gene chip containing 4000 cDNA clones was used to identify the differentially expressed genes in the HaCat cells, and then the results were verified by real-time PCR.


RESULTS
Gene chip hybridization showed that 580 of the 4000 full-length human cDNA exhibited differential expression (>or= 2-fold); 253 genes with complete annotation were identified, including 143 genes up-regulated and 110 genes down-regulated. The differentially expressed genes mainly participated into the processes of cell cycle and immunology.


CONCLUSIONS
Microarry technology helps study the third generation of retinoid acid induced gene expression pattern in human KCs in a high through-put way. AT can be used to treat psoriasis by regulating some processes, such as the cell cycle, inflammation, etc.
Liu
Ya-yan
YY
Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Bi
Xin-ling
XL
Mi
Qing-sheng
QS
Gu
Jun
J
D015152:Blotting, Northern; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014212:Tretinoin / Q000494:pharmacology*; D014325:Tromethamine / Q000494:pharmacology*
D014325:Tromethamine; D014212:Tretinoin