A novel procedure for rapid surface functionalisation and mediator loading of screen-printed carbon electrodes.
We report a simple and rapid procedure that leads to incorporation of mediator and introduction of amine functionality onto the surface of screen-printed carbon electrodes (SPCE). The electrodes were doped with cobalt phthalocyanine (CoPc) by enhanced adsorption in a process that uses minimal amounts of this redox mediator as compared with CoPc loaded inks. The CoPc-doped SPCE showed a substantially increased sensitivity to hydrogen peroxide and thiocholine as compared to unmodified electrodes. This greatly facilitated their use as transducers for the construction of amperometric biosensors based on enzymes producing oxidizable products such as hydrogen peroxide or thiols. Immobilisation of enzymes including glucose oxidase, acetylcholinesterase and choline oxidase was achieved through their multi-contact electrostatic interaction with polyethyleneimine (PEI) which was electrodeposited on the surface of CoPc-doped electrodes in one step from ethanolic solution. The efficiency of enzyme immobilisation was shown to depend on the molecular weight of the PEI used, reaching a maximum for 25 kDa PEI. The biosensors shown sensitivity to glucose at 130 nA mM(-1) (LOD 0.15 mM) and to acetylcholine at 70 nA mM(-1) (LOD 0.10mM) under +0.6 V. Detection of glucose has been demonstrated at +0.4V with the sensitivity of 60 nA mM(-1) and LOD of 0.33 mM. Possibility of the inhibition analysis of pesticides has been shown for acetylcholinesterase-based sensors.
Pchelintsev
Nikolay A
NA
Institute of Membrane and System Biology, University of Leeds, Garstang Building, Leeds, West Yorkshire LS2 9JT, UK.
Millner
Paul A
PA
D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D004566:Electrodes; D004593:Electrophorus; D004800:Enzymes, Immobilized / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry; D013379:Substrate Specificity; D013499:Surface Properties; D013860:Thiocholine / Q000737:chemistry; D013997:Time Factors
D004800:Enzymes, Immobilized; D007211:Indoles; D009942:Organometallic Compounds; C063633:cobalt phthalocyanine; D013860:Thiocholine; D002244:Carbon; D011094:Polyethyleneimine; D006861:Hydrogen Peroxide