title: The effect of immune selection on the structure of the meningococcal opa protein repertoire.
abstract: The opa genes of the Gram negative bacterium Neisseria meningitidis encode Opacity-associated outer membrane proteins whose role is to promote adhesion to the human host tissue during colonisation and invasion. Each meningococcus contains 3-4 opa loci, each of which may be occupied by one of a large number of alleles. We analysed the Opa repertoire structure in a large, well-characterised collection of asymptomatically carried meningococci. Our data show an association between Opa repertoire and meningococcal lineages similar to that observed previously for meningococci isolated from cases of invasive disease. Furthermore, these Opa repertoires exhibit discrete, non-overlapping structure at a population level, and yet low within-repertoire diversity. These data are consistent with the predictions of a mathematical model of strong immune selection upon a system where identical alleles may occupy different loci.
authors: Department of Paediatrics, University of Oxford, Centre for Clinical Vaccinology and Tropical Medicine (CCVTM), Churchill Hospital, Oxford, United Kingdom.
authors: Martin J
authors: MJ
authors: Callaghan
authors: Caroline O
authors: CO
authors: Buckee
authors: Keith A
authors: KA
authors: Jolley
authors: Paula
authors: P
authors: Kriz
authors: Martin C J
authors: MC
authors: Maiden
authors: Sunetra
authors: S
authors: Gupta
mesh_terms: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial / Q000737:chemistry*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D054884:Host-Pathogen Interactions; D006801:Humans; D008957:Models, Genetic; D008962:Models, Theoretical; D009345:Neisseria meningitidis / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; C090348:Opa protein, Neisseria