title: Role of hepatic macrophages during the viral haemorrhagic fever induced by African Swine Fever Virus.
abstract: To ascertain the role played by the various liver monocyte-macrophage populations in the course of a viral hemorrhagic fever, fifteen pigs were inoculated intramuscularly with the highly virulent isolate of African Swine Fever Virus (ASFV) España-70 and slaughtered at 1-7 days post-inoculation (dpi). Samples of liver were fixed in different solutions and routinely processed for morphological, immunohistochemical and ultrastructural studies. Viral antigen (vp73) was detected from 3 dpi onward, mainly in circulating monocytes of sinusoid and Kupffer's cells (KC), as well as in portal macrophages and hepatocytes from 5 dpi. Anti-SWC3 immunolabelled cells were increased from 1 dpi, peaking between 3 and 5 dpi, thereafter declining until the end of the experiment. The significant increase in the number of sinusoidal circulating monocytes and KC expressing IL-1alpha, TNFalpha and IL-6 from 1 dpi, confirmed the secretory activation of these cells. The results show that in the course of an ASFV-induced hemorrhagic syndrome, hepatic macrophage populations undergo major quantitative and biosynthetic changes prior to virus detection, suggesting the existence of a mechanism by which the virus concentrates infectable cells, which subsequently spread the virus around the body.
authors: Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain.
authors: Pedro José
authors: PJ
authors: Sánchez-Cordón
authors: José Lorenzo
authors: JL
authors: Romero-Trevejo
authors: Miriam
authors: M
authors: Pedrera
authors: José Manuel
authors: JM
authors: Sánchez-Vizcaíno
authors: María José
authors: MJ
authors: Bautista
authors: José Carlos
authors: JC
authors: Gómez-Villamandos
mesh_terms: D000358:African Swine Fever Virus / Q000502:physiology*; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D017209:Apoptosis; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006482:Hemorrhagic Fevers, Viral / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D007728:Kupffer Cells / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D008099:Liver / Q000473:pathology*; D008297:Male; D009000:Monocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism / Q000473:pathology*
chemical_list: D000956:Antigens, Viral; D016207:Cytokines