title: Fas expression on peritoneal macrophages during continuous ambulatory peritoneal dialysis peritonitis.
abstract: BACKGROUND
  Peritonitis is a common complication of end stage renal failure (ESRF) patients receiving continuous ambulatory peritoneal dialysis (CAPD). Peritoneal macrophage may participate in the activation of specific T cells and in the generation of local cell-mediated immunity to various pathogens. The purpose of this study is to investigate the possible role of macrophage in CAPD patients with peritonitis.
  
  
  METHODS
  We evaluated the expression of Fas receptor (CD95), ICAM-1 (CD54), CD25, and CD69 by two-color flow cytometry on extravasted macrophages from 16 ESRF patients on CAPD with peritonitis (peritonitis-positive) and compared them to 11 ESRF patients on CAPD without peritonitis (peritonitis-negative) and normal controls.
  
  
  RESULTS
  We found an increased expression of CD95, CD54, and CD25 on macrophage in peritonitis-positive group compared to controls (all p < 0.001). In the peritonitis-positive group, the CD95 expression was significantly higher than that of the peritonitis-negative group (p < 0.001). The expression of CD54, CD25, and CD69, however, was not significantly different between the peritonitis-positive and peritonitis-negative CAPD subgroups.
  
  
  CONCLUSIONS
  We found an abnormally increased percentage of macrophage-expressing Fas receptor and ICAM-1, and the percentage of CD95+ macrophage, but not those of other markers, were increased among the subset of CAPD patients with peritonitis. The later finding suggests that this macrophage phenotype is associated with peritonitis occurring in CAPD.
authors: Division of Nephrology, Department of Medicine, Tian-Sheng Memorial Hospital, Tong-Kang, Ping-Tong, Taiwan.
authors: Chen-Yin
authors: CY
authors: Chen
authors: Chan-Yao
authors: CY
authors: Wu
authors: Tsung-Chang
authors: TC
authors: Tsai
authors: Wei Tung
authors: WT
authors: Lin
authors: Wei-Jen
authors: WJ
authors: Lee
authors: Ching-Chyuan
authors: CC
authors: Su
authors: Hung-Chun
authors: HC
authors: Chen
authors: Hung-Che
authors: HC
authors: Chiang
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000628:therapy; D037181:Lectins, C-Type; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*; D010538:Peritonitis / Q000209:etiology / Q000276:immunology / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018799:Intercellular Adhesion Molecule-1; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D019014:fas Receptor; D000099192:CD69 Antigens