A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate.
A novel class II 5-enoylpyruvylshikimate-3-phosphate synthase (EPSPS) was identified from Pseudomonas stutzeri A1501 by complementation of an Escherichia coli auxotrophic aroA mutant. The single amino acid substitution of serine (Ser) for asparagine (Asn)-130 of the A1501 EPSPS enhanced resistance to 200 mM glyphosate. The mutated EPSPS had a 2.5-fold increase for IC(50) [glyphosate] value, a 2-fold increase for K (i) [glyphosate] value, but a K (m) [PEP] value similar to that of wild type. The effect of the single residue mutation on glyphosate resistance was also analyzed using a computer-based three-dimensional model.
Liang
Aimin
A
College of Biological Sciences, China Agricultural University, Beijing 100094, PR China.
Sha
Jiying
J
Lu
Wei
W
Chen
Ming
M
Li
Liang
L
Jin
Dan
D
Yan
Yongliang
Y
Wang
Jin
J
Ping
Shuzhen
S
Zhang
Wei
W
Wang
Yiding
Y
Lin
Min
M
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000737:chemistry / Q000235:genetics*; D019943:Amino Acid Substitution; D005798:Genes, Bacterial; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000494:pharmacology*; D007700:Kinetics; D050505:Mutant Proteins / Q000737:chemistry; D009154:Mutation / Q000235:genetics*; D010802:Phylogeny; D044223:Pseudomonas stutzeri / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000097797:Glyphosate
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D005998:Glycine; D006540:Herbicides; D050505:Mutant Proteins; D000097797:Glyphosate