Hepatocyte growth factor gene therapy for hypertension.
Hepatocyte growth factor (HGF) has mitogenic, motogenic, and morphogenic biological activities as well as helps in regenerating various tissues. In cardiovascular organs, HGF was reported to have anti-apoptotic, anti-fibrotic, and vasodilating effects. HGF has close relationships with hypertension, arteriosclerosis, and heart failure. HGF enhances renal regeneration and suppresses the progression of hypertension. Intramuscular electroporation of the therapeutic gene is a simple, economic, and low toxic method compared with systemic administration of the purified proteins or peptides. We outline the technique of intramuscular electroporation of HGF gene as a remedy for hypertension.
Komamura
Kazuo
K
Department of Cardiovascular Dynamics, Research Institute, National Cardiovascular Center, Suita, Japan.
Miyazaki
Jun-Ichi
J
Imai
Enyu
E
Matsumoto
Kunio
K
Nakamura
Toshikazu
T
Hori
Masatsugu
M
D000818:Animals; D002318:Cardiovascular Diseases / Q000628:therapy; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D053672:Electrochemotherapy / Q000379:methods*; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D017228:Hepatocyte Growth Factor / Q000097:blood / Q000235:genetics* / Q000502:physiology; D006973:Hypertension / Q000503:physiopathology / Q000628:therapy*; D007273:Injections, Intramuscular; D015848:Interleukin-5 / Q000235:genetics; D007763:Lac Operon; D008297:Male; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D015848:Interleukin-5; D017228:Hepatocyte Growth Factor; D001616:beta-Galactosidase