title: Amelogenin sex determination by pyrosequencing of short PCR products.
abstract: We developed an assay, which allows the sex determination of human DNA samples by pyrosequencing of short PCR products. A 48/45-bp stretch including primers of the amelogenin gene with a 3-bp insertion on the Y chromosome was chosen for analysis. In an initial study, we correctly typed 50 male and 50 female DNA samples from unrelated donors. First experiments with forensic samples, which failed in conventional analyses, indicate that this approach might be an advantage when dealing with degraded DNA.
authors: Institute of Legal Medicine, University of Duisburg-Essen, Essen, Germany.
authors: Frank
authors: F
authors: Tschentscher
authors: Ulrich H
authors: UH
authors: Frey
authors: Thomas
authors: T
authors: Bajanowski
mesh_terms: D053523:Amelogenin / Q000235:genetics*; D041322:Chromosomes, Human, Y; D005260:Female; D053803:Forensic Genetics; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D012732:Sex Determination Analysis
chemical_list: D053523:Amelogenin