[Effect of the self-etching adhesives system on human pulp fibroblast].
OBJECTIVE
To compare and evaluate the biocompatibility of three kinds of dentin bonding agents Xeno III (XO), Adper Prompt (AP), Single bond2 (SB) through cell culture in vitro.


METHODS
Three kinds of dentin bonding agents (XO, AP, SB) were applied on the surface of the dental slices which were 5.0 mm in diameter and 0.5 mm in depth. By immersing the slices into the DMEM culture medium, the maceration extracts were obtained. Normal dental pulps of teenagers were collected and human pulp fibroblast was cultured using tissue explant method. The fifth generation pulp cells were exposed to culture medium containing different concentrations of maceration extracts (100.0%, 50.0%, 25.0%, 12.5%) for 24, 72, 120 h. At last, MTT method was used to evaluate the cytotoxicity of the dentin bonding agents on human pulp fibroblast.


RESULTS
The results showed that all three kinds of dentin bonding systems had cytotoxicity to human pulp fibroblast in different degree in vitro. The cytotoxicity of XO and AP was less than SB. The difference was statistically significant (P<0.05).


CONCLUSIONS
The results of cell culture in vitro indicated that total-etching adhesives system has more irritation to pulp than self-etching adhesives system.
Zhang
Ming
M
Department of Endodontics, The Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China.
Feng
Yan
Y
Huang
Xiao-jing
XJ
Lei
Li-shan
LS
Zheng
Bi-qiong
BQ
Lu
You-guang
YG
D000269:Adhesives; D000293:Adolescent; D003782:Dental Pulp; D003804:Dentin; D017220:Dentin-Bonding Agents; D005347:Fibroblasts; D006801:Humans; D019279:Resin Cements
D000269:Adhesives; D017220:Dentin-Bonding Agents; D019279:Resin Cements; C487957:Xeno III