title: Respiratory viruses in HIV-infected patients with suspected respiratory opportunistic infection.
abstract: OBJECTIVE
  To assess the incidence and epidemiological pattern of respiratory viruses in HIV-infected patients and to evaluate their potential clinical impact.
  
  
  METHODS
  A prospective population-based cohort study was conducted at three Swiss university hospitals. Study participants were HIV-infected patients who underwent a bronchoalveolar lavage to rule out an opportunistic event. All bronchoalveolar lavage specimens were screened using a set of real-time reverse transcriptase-polymerase chain reaction assays targeting 17 different respiratory viruses.
  
  
  RESULTS
  Between November 2003 and November 2006, 59 bronchoalveolar episodes from 55 HIV-infected patients were analysed. Eleven of 59 episodes (18.6%) were positive for at least one respiratory virus. Coronavirus OC43 was identified in three cases (27.3%) followed by influenza A in two (18.2%). Parainfluenza virus (PIV) 2, PIV 3, PIV 4, bocavirus, human rhinovirus A and human metapneumovirus were each identified in one case (9%). In the majority of these cases (63.6%) no other concomitant microorganism was isolated.
  
  
  CONCLUSIONS
  Clinical investigation of respiratory viral infections in HIV-infected patients should not be restricted to prototype viruses and also need to target all the different family of viruses as it seems likely that these viruses contribute to pulmonary complications and morbidity in this population.
authors: Division of Infectious Diseases, University Hospitals of Geneva, Geneva, Switzerland. jorge.garbino@hcuge.ch
authors: Jorge
authors: J
authors: Garbino
authors: Sarra
authors: S
authors: Inoubli
authors: Erik
authors: E
authors: Mossdorf
authors: Rainer
authors: R
authors: Weber
authors: Michael
authors: M
authors: Tamm
authors: Paola
authors: P
authors: Soccal
authors: John-David
authors: JD
authors: Aubert
authors: Pierre-Olivier
authors: PO
authors: Bridevaux
authors: Caroline
authors: C
authors: Tapparel
authors: Laurent
authors: L
authors: Kaiser
mesh_terms: D000328:Adult; D000368:Aged; D052660:Bocavirus / Q000235:genetics; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D028962:Coronavirus OC43, Human / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008297:Male; D029121:Metapneumovirus / Q000235:genetics; D008875:Middle Aged; D009894:Opportunistic Infections / Q000821:virology*; D010223:Parainfluenza Virus 2, Human / Q000235:genetics; D010224:Parainfluenza Virus 3, Human / Q000235:genetics; D029482:Parainfluenza Virus 4, Human / Q000235:genetics; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis*; D012141:Respiratory Tract Infections / Q000821:virology*; D012229:Rhinovirus / Q000235:genetics; D013557:Switzerland; D014777:Virus Diseases / Q000821:virology*; D014780:Viruses / Q000235:genetics*
chemical_list: D012333:RNA, Messenger