Curcumin protects against ischemia/reperfusion injury in rat kidneys.
OBJECTIVE
Renal ischemia followed by reperfusion leads to acute renal failure in both native kidneys and renal allograft. We investigated the effect of curcumin on ischemia-reperfusion (I/R) injury and the antioxidant effects of curcumin in rats.


METHODS
Thirty rats were randomly divided into five experimental groups (control, sham, curcumin, I/R and I/R+curcumin, n=6 each). Curcumin was administered (200 mg kg(-1)) orally to curcumin and I/R+curcumin groups for 7 days. Then, the rats were subjected to bilateral renal ischemia for 45 min and followed by reperfusion for 24 h. All rats were killed and kidney function tests, serum and tissue nitric oxide (NO), protein carbonyl (PC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined. Histopathological examinations were also performed.


RESULTS
Curcumin significantly improved the urea and cystatin C levels in I/R+curcumin group compared to I/R group (p<0.05). Reduction of serum GSH-Px was significantly improved by curcumin (p<0.001), but SOD enzyme activity did not alter (p>0.05). Treatment with curcumin also resulted in significant reduction in serum and tissue MDA, NO and PC and for tissue that were increased by renal I/R injury (p<0.001 for serum and p<0.05 for tissue, respectively). In histological examination, the rats treated with curcumin had nearly normal morphology of the kidney.


CONCLUSIONS
Based on our results, it can be concluded that curcumin protects the kidneys against I/R injury via its antioxidant effects.
Bayrak
Omer
O
Department of Urology, Fatih University, School of Medicine, Ankara, Turkey.
Uz
Ebru
E
Bayrak
Reyhan
R
Turgut
Faruk
F
Atmaca
Ali Fuat
AF
Sahin
Semsettin
S
Yildirim
Mehmet Erol
ME
Kaya
Arif
A
Cimentepe
Ersin
E
Akcay
Ali
A
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003474:Curcumin / Q000008:administration & dosage / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use; D007668:Kidney / Q000098:blood supply*; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D016896:Treatment Outcome
D000975:Antioxidants; D004791:Enzyme Inhibitors; D003474:Curcumin