[Search of streptomycin-resistant bacteria in creek water and application of MALDI-TOF MS to grouping of the isolated bacteria].
A search of streptomycin-resistant bacteria was carried out using ten creek water samples collected in Saga prefecture by spreading the sample water on an R2A medium containing 10 microg/ml of streptomycin. It was clarified that such streptomycin-resistant bacteria as Bacillus, Novosphigobium, Sphingopyxis and Oceanobacillus were distributed in the creek water. Further, 60% of the isolates didn't form an inhibitory zone by the application of 700 microg/ml streptomycin solution in the cup method assay. Further, the effectiveness of the MALDI-TOF MS analysis for the grouping of the isolates was examined. The discriminating ability of MALDI-TOF MS analysis was higher than that of RFLP analysis and it was almost equal to that of sequence analysis using 16S rDNA. Considering the high-throughput ability of the MALDI-TOF MS instrument, MALDI-TOF mass spectral identification of bacteria will be a powerful method in the construction of a MALDI-TOF mass spectra database.
Ichiki
Yayoi
Y
Graduate School of Engineering, Fukuoka Institute of Technology. Higashi-ku, Fukuoka 811-0295, Japan.
Aoki
Tomohiro
T
Takashima
Yasuto
Y
Tamura
Hiroto
H
Teramoto
Kanae
K
Sato
Hiroaki
H
Arashidani
Keiichi
K
Yoshikawa
Hiromichi
H
D016573:Agrochemicals / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification*; D024881:Drug Resistance, Bacterial; D012150:Polymorphism, Restriction Fragment Length; D045483:Rivers / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D013307:Streptomycin / Q000494:pharmacology*
D016573:Agrochemicals; D000900:Anti-Bacterial Agents; D013307:Streptomycin