title: Dendritic cells in colonic patches and iliac lymph nodes are essential in mucosal IgA induction following intrarectal administration via CCR7 interaction.
abstract: This study examined dendritic cells (DC) following intrarectal (IR) vaccination with the mucosal adjuvant cholera toxin (CT). Three rounds of IR vaccination with ovalbumin (OVA) and CT resulted in brisk levels of systemic and mucosal Ig responses. Immunohistochemical studies revealed that CD11c+ MHC class II+ cells accumulated primarily in the colonic patches (CP) and lamina propria of the large intestine (LI-LP), iliac LN (ILN) and MLN following IR vaccination with CT. Adoptively transferred CFSE-labeled OVA-specific CD4+ T cells proliferated significantly, secreting predominantly Th1-type cytokines in the CP (48 h after IR vaccination with CT) and Th2-type cytokines in the ILN (96 h after IR vaccination with CT). Following three IR vaccinations, CP-null mice that were generated by in utero treatment with anti-IL-7R Ab showed reduced levels of serum IgG and fecal IgA antibodies, suggesting a crucial role for CP in the initiation of systemic and mucosal immune responses. Of most interest, IR vaccination reduced IgA levels in fecal extracts significantly more in the CCR7-/- mice than in the wild-type mice. These results indicate that IR vaccination primarily mobilizes CD11c+ cells in the CP and ILN to induce optimal mucosal immune responses by CCR7 interaction.
authors: Mucosal Immunology Section, International Vaccine Institute, Seoul, Korea.
authors: Ah-Young
authors: AY
authors: Lee
authors: Sun-Young
authors: SY
authors: Chang
authors: Jung-Im
authors: JI
authors: Kim
authors: Hye-Ran
authors: HR
authors: Cha
authors: Myoung Ho
authors: MH
authors: Jang
authors: Masafumi
authors: M
authors: Yamamoto
authors: Mi-Na
authors: MN
authors: Kweon
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D039521:CD11c Antigen / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D003106:Colon / Q000166:cytology / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007085:Ilium / Q000166:cytology / Q000276:immunology*; D007070:Immunoglobulin A / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D054400:Receptors, CCR7 / Q000276:immunology*; D012007:Rectum / Q000166:cytology / Q000276:immunology*; D014611:Vaccination
chemical_list: D000941:Antigens; D039521:CD11c Antigen; C515371:Ccr7 protein, mouse; D000949:Histocompatibility Antigens Class II; D007070:Immunoglobulin A; D054400:Receptors, CCR7