Molecular typing of Streptococcus agalactiae isolates from fish.
The genetic variability among Streptococcus agalactiae isolates recovered from fish was characterized using single-stranded conformation polymorphism (SSCP) analysis of the intergenic spacer region (ISR), and amplified fragment length polymorphism (AFLP) fingerprinting. A total of 46 S. agalactiae cultures isolated from different fish species and geographic origins as well as related reference strains were included in the study. ISR-SSCP divided the S. agalactiae isolates analysed into five distinct genotypes. Genotype 1 grouped all Kuwait isolates while genotype 4 clustered the majority of non-Kuwait isolates (USA, Brazil and Honduras). AFLP analysis offered a higher resolution level by dividing the isolates into 13 different genotypes. Two different AFLP profiles were identified within the Kuwait isolates. When data from both ISR-SSCP and AFLP were combined through a multidimensional analysis (MDS), a good correlation between geographical origin and genotypes was observed. Both AFLP and ISR-SSCP revealed genetic differences between S. agalactiae isolates from fish. While AFLP offered a higher resolution, ISR-SSCP also provided valid information being a simpler and faster method.
Olivares-Fuster
O
O
Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, AL 36849, USA.
Klesius
P H
PH
Evans
J
J
Arias
C R
CR
D054458:Amplified Fragment Length Polymorphism Analysis / Q000662:veterinary; D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary*; D016000:Cluster Analysis; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013292:Streptococcus agalactiae / Q000235:genetics* / Q000302:isolation & purification
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S