The unique localization of ZFP185 at uropod of mouse T lymphocytes.
Zinc finger protein 185 (ZFP185), which contains a conservative Lin-l 1, Isl-1 and Mec-3 (LIM) domain at its C-terminus, is a member of mouse LIM protein family. Although brahma-related gene 1(BRG1), the upstream regulation gene of ZFP185, is essential in the development and activation of T lymphocytes, there is no report on the involvement of ZFP185 in lymphocyte activation. The expression of ZFP185 protein was up-regulated significantly in T lymphocytes during their activation by phorbol 12-myristate 13-acetate (PMA)/ionomycin, compared with non-activated T lymphocytes, as determined by western blot assays. However, over-expression of ZFP185 in EL4 cells showed no significant effect on the cell proliferation. Importantly, expression of ZFP185-enhanced green fluorescent protein fusion protein in EL4 cells showed that ZFP185 uniquely localized in the uropod of lymphocytes as observed with a two-photon laser scanning confocal microscope. The direct interaction between ZFP185 and actin was supported by co-immunoprecipitation and immunofluorescence assays. The special distribution of ZFP185 in uropod indicates the potential involvement of ZFP185 in T-cell morphology and movement.
Wang
N
N
Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Zhang
J-S
JS
Zheng
Q
Q
Zhao
Y
Y
D000199:Actins / Q000378:metabolism; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D053645:Interleukin-2 Receptor alpha Subunit; D015759:Ionomycin / Q000494:pharmacology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011554:Pseudopodia / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000199:Actins; D004268:DNA-Binding Proteins; D053645:Interleukin-2 Receptor alpha Subunit; C484614:Zfp185 protein, mouse; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate