A West Nile virus (WNV) recombinant canarypox virus vaccine elicits WNV-specific neutralizing antibodies and cell-mediated immune responses in the horse.
Successful vaccination against West Nile virus (WNV) requires induction of both neutralizing antibodies and cell-mediated immune responses. In this study, we have assessed the ability of a recombinant ALVAC-WNV vaccine (RECOMBITEK WNV) to elicit neutralizing antibodies and virus-specific cell-mediated immune responses in horses. In addition, we examined whether prior exposure to ALVAC-WNV vaccine would inhibit B and cell-mediated immune responses against the transgene product upon subsequent booster immunizations with the same vaccine. The results demonstrated that the recombinant ALVAC-WNV vaccine induced neutralizing antibodies and prM/E insert-specific IFN-gamma(+) producing cells against WNV in vaccinated horses. Prior exposure to ALVAC-WNV vaccine did not impair the ability of horses to respond to two subsequent booster injections with the same vaccine, although anti-vector-specific antibody and cell-mediated immune responses were induced in vaccinated horses. This report describes, for the first time, the induction of antigen-specific cell-mediated responses following vaccination with an ALVAC virus recombinant vaccine encoding WNV antigens. Moreover, we showed that both WNV-specific IFN-gamma producing cells and anti-WNV neutralizing antibody responses, are not inhibited by subsequent vaccinations with the same vector vaccine.
El Garch
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H
Merial SAS, R&D, 254 rue Marcel Mérieux, 69342 Cedex 07 Lyon, France.
Minke
J M
JM
Rehder
J
J
Richard
S
S
Edlund Toulemonde
C
C
Dinic
S
S
Andreoni
C
C
Audonnet
J C
JC
Nordgren
R
R
Juillard
V
V
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D006736:Horses / Q000276:immunology*; D007117:Immunization, Secondary / Q000662:veterinary; D007371:Interferon-gamma / Q000097:blood; D008297:Male; D009500:Neutralization Tests / Q000662:veterinary; D018709:Statistics, Nonparametric; D014611:Vaccination / Q000379:methods / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014901:West Nile Fever / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D053060:West Nile Virus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014902:West Nile virus / Q000276:immunology*
C078788:ALVAC vaccine; D000914:Antibodies, Viral; D014765:Viral Vaccines; D053060:West Nile Virus Vaccines; D007371:Interferon-gamma