title: Rapid detection of anti-Lu(b) with recombinant Lu(b) protein and the particle gel immunoassay.
abstract: BACKGROUND
  Until now, it was not possible to identify antibodies to red blood cells (RBCs) except with pretyped RBCs. Here, a novel method with particles coated with recombinant Lu(b) protein for detection of anti-Lu(b) is described.
  
  
  METHODS
  Prokaryotic recombinant Lu(b) proteins were generated and coupled onto superparamagnetic particles coated with streptavidin. The coated particles were tested in the presence of different serum and plasma samples (13 anti-Lu(b), 6 anti-Lu(a), 20 other antibodies, and 35 serum samples from blood donors) with the particle gel immunoassay (ID-PaGIA).
  
  
  RESULTS
  Lu(b)-coated particles reacted with all 13 samples containing anti-Lu(b), but not with any samples lacking anti-Lu(b). In addition, the anti-Lu(b) titers were higher with Lu(b)-coated particles than with Lu(a-b+) RBCs in almost all cases.
  
  
  CONCLUSIONS
  Recombinant blood group proteins may be able to dispense with the need for RBCs for identification of certain RBC alloantibodies.
authors: Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany. seltsam.axel@mh-hannover.de
authors: Axel
authors: A
authors: Seltsam
authors: Ashraf
authors: A
authors: Agaylan
authors: Daniela
authors: D
authors: Grueger
authors: Oliver
authors: O
authors: Meyer
authors: Rainer
authors: R
authors: Blasczyk
authors: Abdulgabar
authors: A
authors: Salama
mesh_terms: D007118:Immunoassay / Q000379:methods*; D007518:Isoantibodies / Q000097:blood*; D008188:Lutheran Blood-Group System / Q000276:immunology; D011387:Prokaryotic Cells / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology*; D015203:Reproducibility of Results
chemical_list: D007518:Isoantibodies; D008188:Lutheran Blood-Group System; D011994:Recombinant Proteins