title: PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever.
abstract: PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.
authors: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors: Haim
authors: H
authors: Levy
authors: Souleymane
authors: S
authors: Diallo
authors: Sharon M
authors: SM
authors: Tennant
authors: Sofie
authors: S
authors: Livio
authors: Samba O
authors: SO
authors: Sow
authors: Milagritos
authors: M
authors: Tapia
authors: Patricia I
authors: PI
authors: Fields
authors: Matthew
authors: M
authors: Mikoleit
authors: Boubou
authors: B
authors: Tamboura
authors: Karen L
authors: KL
authors: Kotloff
authors: Rosanna
authors: R
authors: Lagos
authors: James P
authors: JP
authors: Nataro
authors: James E
authors: JE
authors: Galen
authors: Myron M
authors: MM
authors: Levine
mesh_terms: D000293:Adolescent; D000942:Antigens, Bacterial / Q000235:genetics; D016470:Bacteremia / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D005798:Genes, Bacterial; D006801:Humans; D010284:Paratyphoid Fever / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012482:Salmonella paratyphi A / Q000235:genetics / Q000302:isolation & purification*; D012484:Salmonella paratyphi B / Q000235:genetics / Q000302:isolation & purification*; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014435:Typhoid Fever / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial