[Rapamycin combined with donor bone marrow-derived immature dendritic cells induces mouse skin allograft tolerance].
OBJECTIVE
To investigate the synergic effects of rapamycin and donor bone marrow-derived immature dendritic cells (DCs) in inducing skin allograft tolerance in mice.


METHODS
The recipient BALB/c mice receiving transplantation of skin allograft from C57BL/6 mice were divided into control group (without perioperative treatments), rapamycin group (receiving rapamycin at 1 mg.kg(-1).d(-1) by gavage for 7 consecutive 7 days after skin transplantation), immature DC group (receiving an injection of donor bone marrow-derived immature DCs of 2 x 10(6) via tail vein before skin transplantation), combined group (receiving an injection of the DCs of 2 x 10(6) before transplantation and rapamycin at 1 mg.kg(-1).d(-1) for 7 consecutive days after transplantation). The survival time of the skin allograft was observed in each group.


RESULTS
The survival time of the skin allograft in the control, rapamycin, immature DC and immature DC +rapamycin groups were 6.9-/+1.9, 12.3-/+3.0, 17.0-/+3.4 and 20.8-/+3.6 days, respectively, showing significant differences among the groups (P<0.05), and SNK test also indicated significant differences between every two groups.


CONCLUSIONS
Rapamycin and donor bone marrow-derived immature DCs have synergic effects in inducing skin allograft tolerance in mice.
Yu
Si
S
Department of Organ Transplantation, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
He
Xiaoshun
X
Hu
Anbin
A
Fu
Bi-mang
BM
Ma
Yi
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D006085:Graft Survival / Q000187:drug effects* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020123:Sirolimus / Q000494:pharmacology*; D016038:Skin Transplantation / Q000276:immunology* / Q000379:methods; D014184:Transplantation, Homologous
D007166:Immunosuppressive Agents; D020123:Sirolimus