title: Crystal structure of D351A and P312A mutant forms of the mammalian sarcoplasmic reticulum Ca(2+) -ATPase reveals key events in phosphorylation and Ca(2+) release.
abstract: In recent years crystal structures of the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA1a), stabilized in various conformations with nucleotide and phosphate analogs, have been obtained. However, structural analysis of mutant forms would also be valuable to address key mechanistic aspects. We have worked out a procedure for affinity purification of SERCA1a heterologously expressed in yeast cells, producing sufficient amounts for crystallization and biophysical studies. We present here the crystal structures of two mutant forms, D351A and P312A, to address the issue whether the profound functional changes seen for these mutants are caused by major structural changes. We find that the structure of P312A with ADP and AlF(4)(-) bound (3.5-A resolution) and D351A with AMPPCP or ATP bound (3.4- and 3.7-A resolution, respectively) deviate only slightly from the complexes formed with that of wild-type ATPase. ATP affinity of the D351A mutant was very high, whereas the affinity for cytosolic Ca(2+) was similar to that of the wild type. We conclude from an analysis of data that the extraordinary affinity of the D351A mutant for ATP is caused by the electrostatic effects of charge removal and not by a conformational change. P312A exhibits a profound slowing of the Ca(2+)-translocating Ca(2)E1P-->E2P transition, which seems to be due to a stabilization of Ca(2)E1P rather than a destabilization of E2P. This can be accounted for by the strain that the Pro residue induces in the straight M4 helix of the wild type, which is removed upon the replacement of Pro(312) with alanine in P312A.
authors: Commissariat à l'Energie Atomique (CEA), Institut de Biologie et de Technologies de Saclay, SBSM, URA CNRS 2096, Laboratoire de Recherche Associé, 17V University of Paris-Sud, Gif sur Yvette, France.
authors: Alexandre
authors: A
authors: Marchand
authors: Anne-Marie Lund
authors: AM
authors: Winther
authors: Peter Joakim
authors: PJ
authors: Holm
authors: Claus
authors: C
authors: Olesen
authors: Cedric
authors: C
authors: Montigny
authors: Bertrand
authors: B
authors: Arnou
authors: Philippe
authors: P
authors: Champeil
authors: Johannes D
authors: JD
authors: Clausen
authors: Bente
authors: B
authors: Vilsen
authors: Jens Peter
authors: JP
authors: Andersen
authors: Poul
authors: P
authors: Nissen
authors: Christine
authors: C
authors: Jaxel
authors: Jesper Vuust
authors: JV
authors: Møller
authors: Marc
authors: M
authors: le Maire
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D018360:Crystallography, X-Ray; D008274:Magnesium / Q000378:metabolism; D008958:Models, Molecular; D050505:Mutant Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010766:Phosphorylation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012519:Sarcoplasmic Reticulum / Q000201:enzymology* / Q000235:genetics; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D050505:Mutant Proteins; D000255:Adenosine Triphosphate; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D008274:Magnesium; D002118:Calcium