title: Determination of danshensu in rat plasma and tissues by high-performance liquid chromatography.
abstract: A systematic study on pharmacokinetics and tissue distribution of danshensu (one of the major active components from Salvia miltiorrhizae) is conducted using a rapid and sensitive high-performance liquid chromatographic (HPLC) method. Before HPLC analysis, biological samples are pretreated with a liquid-liquid extraction. Separation of danshensu and internal standard is achieved on an Agilent Zorbax C18 column with a mobile phase made up of acetonitrile and 0.05% trifluoracetic acid at a flow rate of 0.8 mL/min. The calibration curves in plasma and tissues are linear in the given concentration ranges, with r2 no less than 0.99. The intra-day and inter-day relative standard deviations in the measurement of quality control samples are less than 15%, and the accuracies are in the range of 86-115%. The recoveries of danshensu in plasma and tissues are among 80% to 118%. Meanwhile, the multi-peaks in pharmacokinetic profiles are observed. The method is successfully applied to the pharmacokinetics and tissue distribution study of danshensu after a single oral administration of 50.0 mg/kg sodium danshensu to rats.
authors: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
authors: Zi-chuan
authors: ZC
authors: Zhang
authors: Man
authors: M
authors: Xu
authors: Shi-feng
authors: SF
authors: Sun
authors: Hui
authors: H
authors: Guo
authors: Xue
authors: X
authors: Qiao
authors: Jian
authors: J
authors: Han
authors: Bao-rong
authors: BR
authors: Wang
authors: De-an
authors: DA
authors: Guo
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007668:Kidney / Q000378:metabolism; D007773:Lactates / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid