Genomic variability among high pristinamycin-producing recombinants of Streptomyces pristinaespiralis revealed by amplified fragment length polymorphism.
Amplified fragment length polymorphism (AFLP) was used to analyze genomic variability between high pristinamycin-producing recombinants of Streptomyces pristinaespiralis produced by genome shuffling and their ancestral strain. The AFLP fingerprints obtained with two restriction enzyme combinations of ApaI/TaqI and PstI/SacII showed together that there was no major polymorphism (less than 10%) between these high yield recombinants and their ancestor. However, the unique polymorphic bands, which might be related to the yield increasing of pristinamycin, could be distinguished from all the recombinants. Clustering analysis further indicated that the recombinants with similar ability of pristinamycin production had similar genomic variability.
Jin
Qingchao
Q
Department of Biological and Pharmaceutical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China.
Jin
Zhihua
Z
Xu
Bo
B
Wang
Qiang
Q
Lei
Yinlin
Y
Yao
Shanjing
S
Cen
Peilin
P
D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D014644:Genetic Variation; D016680:Genome, Bacterial / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D025762:Pristinamycin / Q000096:biosynthesis*; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial; D025762:Pristinamycin