title: In vitro production of azadirachtin from cell suspension cultures of Azadirachta indica.
abstract: The present study aimed to elucidate the effect of nutritional alteration on biomass content and azadirachtin production in cell suspensions of the elite neem variety crida-8. Variations in total nitrogen availability in the medium in terms of different ratios of nitrate: ammonium showed that the ratio 4:1 revealed a profound effect, leading to a 1.5-fold increase in the total extracellular azadirachtin production (5.59 mg/l) over the standard MS medium. Reduction in sucrose (15 mg/l) in the medium exhibited a reduction in biomass and absence of azadirachtin, whereas total phosphate reduction raised intracellular azadirachtin production (6.98 mg/l). An altered medium with a nitrate: ammonium ratio of 4:1 coupled with complete elimination of phosphate enhanced biomass by 36% (59.36 g/l).
authors: Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Anantapur Campus, Anantapur 51500, AP, India.
authors: S
authors: S
authors: Sujanya
authors: B Poornasri
authors: BP
authors: Devi
authors: Isha
authors: I
authors: Sai
mesh_terms: D031601:Azadirachta / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D007700:Kinetics; D036701:Limonins / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D036701:Limonins; C010329:azadirachtin