title: Quantitative RT-PCR analysis of pacifastin-related precursor transcripts during the reproductive cycle of solitarious and gregarious desert locusts.
abstract: In locusts, little is known about the physiological and biochemical mechanisms regulating complex processes, such as reproduction and phase transition. The pacifastin family constitutes a family of peptidic inhibitors of serine proteases that are considered to be important regulators of several physiological processes in arthropods. We have performed a detailed transcript profiling analysis of two pacifastin-related peptide precursors, SGPP-2 and SGPP-4, during the reproductive cycle of adult desert locusts (Schistocerca gregaria). This quantitative real-time (RT)-PCR analysis revealed a temporal regulation of both transcripts, which is paralleled by several events that occur during the reproductive cycle of adult locusts. The observed temporal transcript profiles display a strong tissue-, gender- and phase-dependence. In addition, a partial regregarization experiment suggests that both transcript levels are regulated during phase transition and can be employed as molecular markers of the gregarization process.
authors: Department of Animal Physiology and Neurobiology, Zoological Institute K.U. Leuven, Leuven, Belgium. bert.breugelmans@bio.kuleuven.be
authors: B
authors: B
authors: Breugelmans
authors: G
authors: G
authors: Simonet
authors: V
authors: V
authors: van Hoef
authors: I
authors: I
authors: Claeys
authors: S
authors: S
authors: Van Soest
authors: J
authors: J
authors: Vanden Broeck
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D006110:Grasshoppers / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008297:Male; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012098:Reproduction / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D011498:Protein Precursors; D011506:Proteins; D012333:RNA, Messenger; C106709:pacifastin