title: Recent advances in the characterization of HIV-1 neutralization assays for standardized evaluation of the antibody response to infection and vaccination.
abstract: In AIDS vaccine development the pendulum has swung towards a renewed emphasis on the potential role for neutralizing antibodies in a successful global vaccine. It is recognized that vaccine-induced antibody performance, as assessed in the available neutralization assays, may well serve as a "gatekeeper" for HIV-1 subunit vaccine prioritization and advancement. As a result, development of a standardized platform for reproducible measurement of neutralizing antibodies has received considerable attention. Here we review current advancements in our knowledge of the performance of different types of antibodies in a traditional primary cell neutralization assay and the newer, more standardized TZM-bl reporter cell line assay. In light of recently revealed differences (see accompanying article) in the results obtained in these two neutralization formats, parallel evaluation with both platforms should be contemplated as an interim solution until a better understanding of immune correlates of protection is achieved.
authors: Walter Reed Army Institute of Research, Washington, DC, USA. vpolonis@hivresearch.org
authors: Victoria R
authors: VR
authors: Polonis
authors: Bruce K
authors: BK
authors: Brown
authors: Andrew
authors: A
authors: Rosa Borges
authors: Susan
authors: S
authors: Zolla-Pazner
authors: Dimiter S
authors: DS
authors: Dimitrov
authors: Mei-Yun
authors: MY
authors: Zhang
authors: Susan W
authors: SW
authors: Barnett
authors: Ruth M
authors: RM
authors: Ruprecht
authors: Gabriella
authors: G
authors: Scarlatti
authors: Eva-Maria
authors: EM
authors: Fenyö
authors: David C
authors: DC
authors: Montefiori
authors: Francine E
authors: FE
authors: McCutchan
authors: Nelson L
authors: NL
authors: Michael
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology*; D000918:Antibody Specificity; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015658:HIV Infections / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D014611:Vaccination
chemical_list: D016915:AIDS Vaccines; D015483:HIV Antibodies; D008156:Luciferases