title: Cellular studies of MrDb (DDX18).
abstract: Helicases are present in viruses, prokaryotes, and eukaryotes, and several of them have been linked to human diseases. Here we study the role of one putative DEAD-box RNA helicase, MrDb (DDX18), in tumor cells. We show that MrDb is a nucleolar protein ubiquitously expressed in tumor cells and that it is more abundantly expressed in proliferating cells. Inhibition of MrDb with dominant negative mutant or a shRNA reduces tumor cell proliferation without inducing a cell cycle arrest or apoptosis. These findings suggest that MrDb is important for cell proliferation and that its inhibition could prevent tumor cell proliferation.
authors: Druggability-Enzymology-Profiling Unit, Oncology Research, Novartis Institutes for BioMedical Research, CH-4002 Basel, Switzerland.
authors: Sandy
authors: S
authors: Dubaele
authors: Patrick
authors: P
authors: Chène
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D016503:Drug Delivery Systems; D015870:Gene Expression / Q000502:physiology*; D006801:Humans; D009154:Mutation; D012313:RNA / Q000378:metabolism
chemical_list: D012313:RNA; C529049:DDX18 protein, human; D053487:DEAD-box RNA Helicases