[Establishment of a model of intracellular infection on human dental pulp fibroblast by Porphyromonas gingivalisin vitro].
OBJECTIVE
To establish a model of intracellular infection of human dental pulp fibroblast (HDPF) by Porphyromonas gingivalis in vitro.


METHODS
HDPF was incubated with live Porphyromonas gingivalis with a multiplicitas of infection (MOI) from 100 for 0.5, 1.0, 2.0 hours. HDPF infected with live Porphyromonas gingivalis were observed by inverted microscope. Metronidazole, Penicillin, Streptomycin were added to the medium to kill any extracellular bacteria. Then the cells were lysed by sterile distilled water, dilutions of the lysates of cells were cultured anaerobically. Colony of invasive bacteria were then observed. HDPF exposed live Porphyromonas gingivalis with a MOI 50 and 100 for 1.0 hour, then cells survival rate were detected by MTT assay.


RESULTS
After infected with Porphyromonas gingivalis 2.0 hours, cells were eumorphism. Porphyromonas gingivalis of incubation for 1.0, 2.0 hours was invasive cells. MTT assays indicated 99.07% cells survival rate with a MOI from 50 and 74.43% cells survival rate with a MOI from 100.


CONCLUSIONS
A model of HDPF intracellular infection with Porphyromonas gingivalis was successfully established in vitro.
Lu
Yu
Y
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Luo
Shi-gao
SG
Huang
Ding-ming
DM
D003782:Dental Pulp; D005347:Fibroblasts; D006801:Humans; D008795:Metronidazole; D017861:Porphyromonas; D016966:Porphyromonas gingivalis
D008795:Metronidazole