Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance.
Histone modification in chromatin is one of the key control points in gene regulation in eukaryotic cells. Protein complexes composed of histone acetyltransferase or deacetylase, WD40 repeat protein, and many other components have been implicated in this process. Here, we report the identification and functional characterization of HOS15, a WD40-repeat protein crucial for repression of genes associated with abiotic stress tolerance through histone deacetylation in Arabidopsis. HOS15 shares high sequence similarity with human transducin-beta like protein (TBL), a component of a repressor protein complex involved in histone deacetylation. Mutation of the HOS15 gene renders mutant plants hypersensitive to freezing temperatures. HOS15 is localized in the nucleus and specifically interacts with histone H4. The level of acetylated histone H4 is higher in the hos15 mutant than in WT plants. Moreover, the stress inducible RD29A promoter is hyperinduced and associated with a substantially higher level of acetylated histone H4 in the hos15 mutant under cold stress conditions. Our results suggest a critical role for gene activation/repression by histone acetylation/deacetylation in plant acclimation and tolerance to cold stress.
Zhu
Jianhua
J
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Jeong
Jae Cheol
JC
Zhu
Yanmei
Y
Sokolchik
Irina
I
Miyazaki
Saori
S
Zhu
Jian-Kang
JK
Hasegawa
Paul M
PM
Bohnert
Hans J
HJ
Shi
Huazhong
H
Yun
Dae-Jin
DJ
Bressan
Ray A
RA
D000107:Acetylation; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003080:Cold Temperature; D005615:Freezing; D018506:Gene Expression Regulation, Plant; D006657:Histones / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000302:isolation & purification; D009154:Mutation / Q000235:genetics; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D012097:Repressor Proteins / Q000378:metabolism; D015533:Transcriptional Activation
D029681:Arabidopsis Proteins; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; C528184:Hos15 protein, Arabidopsis; D050505:Mutant Proteins; D012097:Repressor Proteins; D008156:Luciferases