Scanning for DNA variants by denaturant capillary electrophoresis.
Analysis and detection of DNA variation is important in any field of biology. Hence, numerous methods have been developed to analyze DNA. A simple yet effective way of analyzing DNA is by denaturant capillary electrophoresis (DCE). The method is in theory applicable to 95% of the human genome. The method involves three steps; fragment design, PCR amplification and allele separation. The allele separation can in principle be performed with any DNA capillary sequencing instrument.
Ekstrøm
Per O
PO
The Norwegian Radium Hospital, Oslo, Norway.
D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D015894:Genome, Human; D006801:Humans; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction
D004247:DNA