[Regulatory properties of Bacillus subtilis isolectins].
The ability of natural and mutant Bacillus subtilis cultures with imperfect reparation/recombination system to synthesis of extracellular and surface lectins was investigated, and dependence of lectin production process on cultures' genotype was proved. Mutant B. subtilis recP has practically lost its ability to produce the extracellular lectins as a result of mutation of a gene of the reparation/recombination system. The application of the method "autofocusing" allowed to investigate all the spectrum oflectin molecular forms of natural B. subtilis culture and to reveal isoforms distinguished by physico-chemical and hemagglutination properties. It was shown that lectin cathode forms inhibit the transcription process from plasmid promnoter completely, and anodic forms activate the transcript formation slightly in the transcription in vitro with T7 bacteriophage DNA-dependent RNA-polymerase.
Pidhors'kyĭ
V S
VS
Sashchuk
O V
OV
Karpova
I S
IS
Kovalenko
E O
EO
Korets'ka
N V
NV
Pal'chykovs'ka
L H
LH
Het'man
K I
KI
D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015536:Down-Regulation; D004912:Erythrocytes / Q000187:drug effects; D006386:Hemagglutination Tests; D007525:Isoelectric Focusing; D037102:Lectins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D009154:Mutation; D014158:Transcription, Genetic
D037102:Lectins; D012321:DNA-Directed RNA Polymerases