A reliable high-performance liquid chromatography with ultraviolet detection for the determination of sulfonamides in honey.
The sulfonamides are stable chemotherapeutics used against the bacterial disease affecting bees, known as American foulbrood (Bacillus larvae), so their residues could appear in the honey of treated bees. Their presence at a concentration above the limit value is a potential hazard to human health. Brazilian authorities have included in the National regulatory monitoring program, the control of the three most widely used sulfonamides in honey production, i.e., sulfathiazole, sulfamethazine and sulfadimethoxine. A method for the determination of residual sulfonamides in honey, using sulfapyridine as an internal standard has been developed, optimized and validated. Some changes were implemented on current available methodologies for the analysis of sulfonamides in honey in order to adopt such procedures to Brazilian honey samples. Sulfonamides were extracted from honey with dichloromethane after dissolution with 30% sodium chloride, and cleaned up with solid phase extraction on Florisil columns. The eluate was analyzed by high-performance liquid chromatography with ultraviolet detection. The limit of detection was determined at 3 microg kg(-1), 4 microg kg(-1) and 5 microg kg(-1) for sulfathiazole, sulfamethazine and sulfadimethoxine, respectively with average recoveries of 61.0% for sulfathiazole; 94.5% for sulfamethazine and 86.0% for sulfadimethoxine at the 100 microg kg(-1) level. As the final step of validation procedure, the analysts were submitted to a blind spiked sample prepared by the quality assurance officer which results were successfully obtained regarding recovery and deviations.
Granja
Rodrigo H M M
RH
Microbioticos Laboratories, Av. Santa Isabel 2120, Campinas, SP, 13084-471, Brazil. rodrigo@microbioticos.com
Niño
Alfredo M Montes
AM
Rabone
Fernanda
F
Salerno
Alessandro Gonzalez
AG
D002241:Carbohydrates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D006722:Honey / Q000032:analysis*; D006868:Hydrolysis; D008752:Methylene Chloride / Q000737:chemistry; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D052616:Solid Phase Extraction; D000077589:Sulfathiazole; D013432:Sulfathiazoles / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis*; D014466:Ultraviolet Rays
D002241:Carbohydrates; D013432:Sulfathiazoles; D013449:Sulfonamides; D012965:Sodium Chloride; D008752:Methylene Chloride; D000077589:Sulfathiazole