Identification and differentiation of Candida species from pediatric patients by random amplified polymorphic DNA.
Thirty-four Candida isolates were analyzed by random amplified polymorphic DNA using the primer OPG-10:24 Candida albicans; 4 Candida tropicalis; 2 Candida parapsilosis; 2 Candida dubliniensis; 1 Candida glabrata and 1 Candida krusei. The UPGMA-Pearson correlation coefficient was used to calculate the genetic distance between the different Candida groupings. Samples were classified as identical (correlation of 100%); highly related samples (90%); moderately related samples (80%) and unrelated samples (< 70%). The results showed that the RAPD proposed was capable of classifying the isolates coherently (such that same species were in the same dendrogram), except for two isolates of Candida parapsilosis and the positive control (Netherlands, 1973), probably because they are now recognized as three different species. Concerning the only fluconazole-resistant Candida tropicalis isolate with a genotype that was different to the others, the data were insufficient to affirm that the only difference was the sensitivity to fluconazole. We concluded that the Random Amplified Polymorphic DNA proposed might be used to confirm Candida species identified by microbiological methods.
Rocha
Bruno Aragão
BA
Laboratory of Medical Investigation, Department of Pediatrics, Faculdade de Medicina, Children's Institute, Universidade de São Paulo, Cerqueira César, São Paulo, SP, Brazil.
Negro
Gilda Maria Barbaro del
GM
Yamamoto
Lidia
L
Souza
Mariana Vitule Brito de
MV
Precioso
Alexander Roberto
AR
Okay
Thelma Suely
TS
D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015725:Fluconazole / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D016533:Mycological Typing Techniques; D019105:Random Amplified Polymorphic DNA Technique
D000935:Antifungal Agents; D017931:DNA Primers; D004271:DNA, Fungal; D015725:Fluconazole