title: A Histone2BCerulean BAC transgene identifies differential expression of Phox2b in migrating enteric neural crest derivatives and enteric glia.
abstract: The mammalian enteric nervous system (ENS) derives from migratory enteric neural crest-derived cells (ENCC) that express the transcription factor Phox2b. Studies of these enteric progenitors have typically relied on immunohistochemical (IHC) detection. To circumvent complicating factors of IHC, we have generated a mouse BAC transgenic line that drives a Histone2BCerulean (H2BCFP) reporter from Phox2b regulatory regions. This construct does not alter the endogenous Phox2b locus and enables studies of normal neural crest (NC) derivatives. The Phox2b-H2BCFP transgene expresses the H2BCFP reporter in patterns that recapitulate expression of endogenous Phox2b. Our studies reveal Phox2b expression in mature enteric glia at levels below that of enteric neurons. Moreover, we also observe differential expression of the transgene reporter within the leading ENCC that traverse the gut. Our findings indicate that the wavefront of migrating enteric progenitors is not homogeneous, and suggest these cells may be fate-specified before expression of mature lineage markers appears.
authors: Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0275, USA.
authors: Jennifer C
authors: JC
authors: Corpening
authors: V Ashley
authors: VA
authors: Cantrell
authors: Karen K
authors: KK
authors: Deal
authors: E Michelle
authors: EM
authors: Southard-Smith
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics / Q000378:metabolism; D017615:Enteric Nervous System / Q000033:anatomy & histology / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000196:embryology / Q000294:innervation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009432:Neural Crest / Q000166:cytology / Q000502:physiology; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes
chemical_list: D006657:Histones; D018398:Homeodomain Proteins; C109220:NBPhox protein; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors