Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues.
Plants are the organisms containing the most complex multigenic family for thioredoxins (TRX). Several types of TRXs are targeted to chloroplasts, which have been classified into four subgroups: m, f, x, and y. Among them, TRXs f and m were the first plastidial TRXs characterized, and their function as redox modulators of enzymes involved in carbon assimilation in the chloroplast has been well-established. Both TRXs, f and m, were named according to their ability to reduce plastidial fructose-1,6-bisphosphatase (FBPase) and malate dehydrogenase (MDH), respectively. Evidence is presented here based on the immunocytochemistry of the localization of f and m-type TRXs from Pisum sativum in non-photosynthetic tissues. Both TRXs showed a different spatial pattern. Whilst PsTRXm was localized to vascular tissues of all the organs analysed (leaves, stems, and roots), PsTRXf was localized to more specific cells next to xylem vessels and vascular cambium. Heterologous complementation analysis of the yeast mutant EMY63, deficient in both yeast TRXs, by the pea plastidial TRXs suggests that PsTRXm, but not PsTRXf, is involved in the mechanism of reactive oxygen species (ROS) detoxification. In agreement with this function, the PsTRXm gene was induced in roots of pea plants in response to hydrogen peroxide.
Traverso
José A
JA
Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), C/ Prof. Albareda 1, E-18008-Granada, Spain. jose.traverso@isv.cnrs-gif.fr
Vignols
Florence
F
Cazalis
Roland
R
Serrato
Antonio J
AJ
Pulido
Pablo
P
Sahrawy
Mariam
M
Meyer
Yves
Y
Cejudo
Francisco Javier
FJ
Chueca
Ana
A
D015870:Gene Expression / Q000187:drug effects; D005816:Genetic Complementation Test; D006861:Hydrogen Peroxide / Q000494:pharmacology; D005810:Multigene Family; D018384:Oxidative Stress; D018532:Pisum sativum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018514:Plant Structures / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013879:Thioredoxins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics
D020033:Protein Isoforms; D013879:Thioredoxins; D006861:Hydrogen Peroxide