Alpha-glucosidase inhibitory activity of Syzygium cumini (Linn.) Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats.
Syzygium cumini seed kernel extracts were evaluated for the inhibition of alpha-glucosidase from mammalian (rat intestine), bacterial (Bacillus stearothermophilus), and yeast (Saccharomyces cerevisiae, baker's yeast). In vitro studies using the mammalian alpha-glucosidase from rat intestine showed the extracts to be more effective in inhibiting maltase when compared to the acarbose control. Since acarbose is inactive against both the bacterial and the yeast enzymes, the extracts were compared to 1-deoxynojirimycin. We found all extracts to be more potent against alpha-glucosidase derived from B. stearothermophilus than that against the enzymes from either baker's yeast or rat intestine. In an in vivo study using Goto-Kakizaki (GK) rats, the acetone extract was found to be a potent inhibitor of alpha-glucosidase hydrolysis of maltose when compared to untreated control animals. Therefore, these results point to the inhibition of alpha-glucosidase as a possible mechanism by which this herb acts as an anti-diabetic agent.
Shinde
Jayantrao
J
Department of Pharmaceutical Sciences, College of Pharmacy and Allied Health Professions, St. John's University, 8000 Utopia Parkway, Jamaica, NY 11439, United States.
Taldone
Tony
T
Barletta
Michael
M
Kunaparaju
Naveen
N
Hu
Bo
B
Kumar
Sunil
S
Placido
Jessica
J
Zito
S William
SW
D020909:Acarbose / Q000494:pharmacology; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000628:therapy*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D065089:Glycoside Hydrolase Inhibitors; D066298:In Vitro Techniques; D007422:Intestines / Q000201:enzymology; D008297:Male; D008320:Maltose / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D011922:Rats, Mutant Strains; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012639:Seeds / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry*
D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D010936:Plant Extracts; D008320:Maltose; D020909:Acarbose