Isolation and structural characterization of a novel oligosaccharide from the rhamnogalacturonan of Gossypium hirsutum L.
An alkali extract of cell walls of Gossypium hirsutum L. was sequentially digested by endo-polygalacturonase (EC 3.2.1.15), arabinofuranosidase AN1571.2 (EC 3.2.1.55), endo-arabinase (EC 3.2.1.99), and rhamnogalacturonan hydrolase AN9314.2 (EC 3.2.1.15). The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, size-exclusion, and anion exchange chromatography. Fractions from the anion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOFMS. A new oligosaccharide (RGS29), which contained a rhamnogalacturonan dimer backbone with two galactose and two arabinose residues in the side chains, was found. Its structure was identified by 1D and 2D NMR spectra as follows: [Formula: see text].
Zheng
Yun
Y
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078-3035, USA.
Mort
Andrew
A
D001089:Arabinose / Q000737:chemistry; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D005690:Galactose / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism; D003368:Gossypium / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides; C042491:rhamnogalacturonan I; D010368:Pectins; D001089:Arabinose; C074451:Endo-arabinase; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; D005690:Galactose