The human homolog of fission yeast Rad17 is implicated in tumor growth.
The Schizosaccharomyces pombe rad17 is a checkpoint protein critical for maintenance of genomic stability. Since the loss of checkpoint control is a common feature of tumor cells, we investigated the biological function of the human homolog hRAD17. Expression of hRAD17 in a fission yeast rad17 deleted strain reduced growth of yeast colonies and caused slower progression through cell cycle. Immunoprecipitated hRad17 exhibited exonuclease activity. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. Our results support that hRAD17, similarly to other human genes involved in checkpoint mechanisms, plays a role in control of tumor growth.
Beretta
Giovanni L
GL
Department of Experimental Oncology and Laboratories, Fondazione IRCCS Istituto Nazionale Tumori, via Venezian 1, 20133 Milan, Italy.
Gatti
Laura
L
Cesare
Michelandrea De
MD
Corna
Elisabetta
E
Tinelli
Stella
S
Carenini
Nives
N
Zunino
Franco
F
Perego
Paola
P
D000818:Animals; D018797:Cell Cycle Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D048708:Cell Growth Processes; D002461:Cell Line, Transformed; D005092:Exonucleases / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D016193:G1 Phase; D017353:Gene Deletion; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000473:pathology; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics
D018797:Cell Cycle Proteins; C113575:Rad17 protein, human; D029702:Schizosaccharomyces pombe Proteins; D005092:Exonucleases