Cutting edge: Langerin+ dendritic cells in the mesenteric lymph node set the stage for skin and gut immune system cross-talk.
Topical transcutaneous immunization (TCI) presents many clinical advantages, but its underlying mechanism remains unknown. TCI induced Ag-specific IgA Ab-secreting cells expressing CCR9 and CCR10 in the small intestine in a retinoic acid-dependent manner. These intestinal IgA Abs were maintained in Peyer's patch-null mice but abolished in the Peyer's patch- and lymph node-null mice. The mesenteric lymph node (MLN) was shown to be the site of IgA isotype class switching after TCI. Unexpectedly, langerin(+)CD8alpha(-) dendritic cells emerged in the MLN after TCI; they did not migrate from the skin but rather differentiated rapidly from bone marrow precursors. Depletion of langerin(+) cells impaired intestinal IgA Ab responses after TCI. Taken together, these findings suggest that MLN is indispensable for the induction of intestinal IgA Abs following skin immunization and that cross-talk between the skin and gut immune systems might be mediated by langerin(+) dendritic cells in the MLN.
Chang
Sun-Young
SY
Mucosal Immunology Section, International Vaccine Institute, Seoul, Republic of Korea.
Cha
Hye-Ran
HR
Igarashi
Osamu
O
Rennert
Paul D
PD
Kissenpfennig
Adrien
A
Malissen
Bernard
B
Nanno
Masanobu
M
Kiyono
Hiroshi
H
Kweon
Mi-Na
MN
D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects; D000954:Antigens, Surface / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D007422:Intestines / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D037241:Mannose-Binding Lectins / Q000276:immunology*; D008643:Mesentery / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000276:immunology*; D014212:Tretinoin / Q000494:pharmacology
D000954:Antigens, Surface; C498497:Cd207 protein, mouse; D007070:Immunoglobulin A; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D014212:Tretinoin