Models of Caenorhabditis elegans infection by bacterial and fungal pathogens.
The nematode Caenorhabditis elegans is a simple model host for studying the relationship between the animal innate immune system and a variety of bacterial and fungal pathogens. Extensive genetic and molecular tools are available in C. elegans, facilitating an in-depth analysis of host defense factors and pathogen virulence factors. Many of these factors are conserved in insects and mammals, indicating the relevance of the nematode model to the vertebrate innate immune response. Here, we describe pathogen assays for a selection of the most commonly studied bacterial and fungal pathogens using the C. elegans model system.
Powell
Jennifer R
JR
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA.
Ausubel
Frederick M
FM
D000818:Animals; D001419:Bacteria; D001424:Bacterial Infections / Q000382:microbiology*; D017173:Caenorhabditis elegans / Q000254:growth & development / Q000382:microbiology*; D004195:Disease Models, Animal; D005658:Fungi / Q000502:physiology*; D008828:Microbiological Techniques / Q000379:methods*; D009181:Mycoses / Q000382:microbiology*; D016019:Survival Analysis