title: Establishment of a young mouse model and identification of an allelic variation of zmpB in complicated pneumonia caused by Streptococcus pneumoniae.
abstract: OBJECTIVE
  Complicated pneumonia, including necrotizing pneumonia, lung abscess, and empyema, caused by Streptococcus pneumoniae in children has been increasing. We thus determined to investigate its virulence in an animal model and to identify virulence factors of S. pneumoniae.
  
  
  METHODS
  Prospective, randomized, controlled animal study.
  
  
  METHODS
  University medical laboratory.
  
  
  METHODS
  Male Balb/c-strain mice, 3 wks old.
  
  
  METHODS
  We used a young mouse model to monitor bacterial virulence and a microarray to compare gene expression between S. pneumoniae from children with complicated and noncomplicated pneumonia. Deletion and complementation of a candidate gene were performed to study its role on the virulence of S. pneumoniae.
  
  
  RESULTS
  A model of complicated pneumonia in young mice infected with strains of S. pneumoniae from children with complicated pneumonia was established. Using a microarray analysis, differences in zinc metalloprotease B (zmpB) RNA hybridization between two strains from children with complicated pneumonia (NTUH-p28 and NTUH-p15) and a strain (NTUH-p3) from a child with pneumococcal lobar pneumonia were found. Confirmatory assays revealed the signal differences were due to sequence variation in the zmpB gene. Infection with the zmpB deletion mutant of NTUH-p15 showed a significant decrease in the severity of pneumonia and no destructive lung injury. The zmpB complementation strain of NTUH-p15 significantly restored the level of inflammation and caused lung necrosis. For studying the effect of allelic variation of zmpB on the virulence of S. pneumoniae, we added zmpB of NTUH-p15 in the zmpB deletion mutant of NTUH-p3, which resulted in a higher bacterial burden than that in wild-type NTUH-p3.
  
  
  CONCLUSIONS
  A young mouse model is established for complicated pneumococcal pneumonia. This model proved that allelic variation of zmpB affects the virulence of S. pneumoniae.
authors: Section of Infection, Department of Medicine, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan.
authors: Yu-Chia
authors: YC
authors: Hsieh
authors: Po-Nien
authors: PN
authors: Tsao
authors: Chi-Long
authors: CL
authors: Chen
authors: Tzu-Lung
authors: TL
authors: Lin
authors: Wen-Sen
authors: WS
authors: Lee
authors: Pei-Lan
authors: PL
authors: Shao
authors: Chin-Yun
authors: CY
authors: Lee
authors: Po-Ren
authors: PR
authors: Hsueh
authors: Li-Min
authors: LM
authors: Huang
authors: Jin-Town
authors: JT
authors: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D002648:Child; D004195:Disease Models, Animal; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D011018:Pneumonia, Pneumococcal / Q000145:classification / Q000382:microbiology*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D012720:Severity of Illness Index; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity
chemical_list: D012329:RNA, Bacterial; C412019:ZmpB enzyme; D008666:Metalloendopeptidases