title: Influence of postprandial triglyceride-rich lipoproteins on lipid-mediated gene expression in smooth muscle cells of the human coronary artery.
abstract: OBJECTIVE
  Postprandial triglyceride-rich lipoproteins (TRL) have a direct effect on vascular smooth muscle cells (SMC) and they increase the risk of atherogenesis. Here, we have tested the hypothesis that the different fatty acid composition of TRL is capable of differentially modifying gene expression in human coronary artery SMC (CASMC). In addition, the effect of TRL on cell proliferation and transcription factor activation was also evaluated.
  
  
  RESULTS
  TRL were prepared from plasma of healthy volunteers after the ingestion of meals enriched in refined olive oil (ROO), butter or a mixture of vegetable and fish oils (VEFO). We use cDNA microarrays to determine the genes differentially expressed in TRL-treated CASMC. Correspondence cluster analysis demonstrated that TRL-butter, -ROO and -VEFO provoked different transcriptional profiles in CASMC. Sixty-six genes were regulated by TRL-butter, 55 by -ROO, and 47 by -VEFO. The data revealed that TRL-butter predominantly activated genes involved in the regulation of cell proliferation and inflammation. Likewise, TRL-VEFO induced the expression of genes implicated in inflammation, while TRL-ROO promoted a less atherogenic gene profile.
  
  
  CONCLUSIONS
  The pathophysiological contribution of TRL to the development of atherosclerosis and the stability of atherosclerotic plaques may depend on the fatty acid composition of TRL. Our findings suggest a role for macrophage-inhibiting cytokine-1 (MIC-1) in coronary artery cardiovascular events.
authors: Cellular and Molecular Nutrition, Instituto de la Grasa, Avda, Padre García Tejero, 4, 41012 Seville, Spain.
authors: Beatriz
authors: B
authors: Bermúdez
authors: Sergio
authors: S
authors: López
authors: Yolanda M
authors: YM
authors: Pacheco
authors: José
authors: J
authors: Villar
authors: Francisco J G
authors: FJ
authors: Muriana
authors: Jöerg D
authors: JD
authors: Hoheisel
authors: Andrea
authors: A
authors: Bauer
authors: Rocío
authors: R
authors: Abia
mesh_terms: D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016000:Cluster Analysis; D003331:Coronary Vessels / Q000187:drug effects / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D055436:Growth Differentiation Factor 15; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D019518:Postprandial Period / Q000502:physiology*; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D016207:Cytokines; D005227:Fatty Acids; C526140:GDF15 protein, human; D055436:Growth Differentiation Factor 15; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D014280:Triglycerides