title: Supplementation of ascorbic acid and alpha-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats.
abstract: Contamination of arsenic in drinking water is associated with several human diseases including cancer. It has been reported that oxidative stress plays a vital role in arsenic-induced biochemical and molecular alterations. The aim of the present study was to improve the understanding of arsenic-induced oxidative damage to proteins and to DNA and the role of antioxidants such as ascorbic acid and alpha-tocopherol in alleviating arsenic-induced damages in experimental rats. A significant increase in the levels of protein oxidation, DNA strand breaks, and DNA-protein cross-links was observed in blood, liver, and kidney of rats exposed to arsenic (100 ppm in drinking water) for 30 days. Co-administration of ascorbic acid and alpha-tocopherol to arsenic-exposed rats showed a substantial reduction in the levels of arsenic-induced oxidative products of protein and DNA. The results of this study support that free radical-mediated toxic manifestations of arsenic and also suggest that ascorbic acid and alpha-tocopherol supplementation can improve the arsenic-induced molecular alterations.
authors: Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India.
authors: R
authors: R
authors: Kadirvel
authors: K
authors: K
authors: Sundaram
authors: S
authors: S
authors: Mani
authors: S
authors: S
authors: Samuel
authors: N
authors: N
authors: Elango
authors: C
authors: C
authors: Panneerselvam
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D020261:Arsenic Poisoning / Q000378:metabolism / Q000517:prevention & control*; D018053:Arsenites / Q000633:toxicity; D001205:Ascorbic Acid / Q000494:pharmacology*; D020552:Comet Assay; D003432:Cross-Linking Reagents; D004249:DNA Damage / Q000187:drug effects*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004791:Enzyme Inhibitors / Q000633:toxicity; D007668:Kidney / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000633:toxicity; D014881:Water Supply; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D018053:Arsenites; D003432:Cross-Linking Reagents; D004791:Enzyme Inhibitors; D017670:Sodium Compounds; C017947:sodium arsenite; D024502:alpha-Tocopherol; D001205:Ascorbic Acid