Polymorphism of the PRNP gene in the main breeds of indigenous Chinese goats.
The polymorphism of the PRNP gene plays a key role in susceptibility to prion disease. Scrapie is a neurodegenerative disease affecting sheep and goats and belongs to the group of prion diseases. We isolated DNA from 333 goat samples representing the main local goat breeds in six provinces in China to identify PRNP polymorphisms and to determine whether these breeds were at risk for developing scrapie. Two novel amino acid polymorphisms (R211G and T219I) and a novel silent mutation at codon 125 as well as nine previously reported polymorphisms were observed. Twenty-eight alleles and forty-nine different genotypes were obtained. The codon 142M associated with resistance of goat scrapie was not found in this study. The codon 143R was relatively rare. The codon 222K, a potentially useful candidate site for selecting for scrapie resistance, was also rare in indigenous Chinese goats. These results could provide some useful data for assessing the risk of scrapie in Chinese indigenous goats.
Zhou
R Y
RY
College of Animal Science and Technology, Agricultural University of Hebei, No. 289 Lingyusi Street, 071001 Baoding, Hebei, People's Republic of China.
Li
X L
XL
Li
L H
LH
Wang
H Y
HY
Lü
J G
JG
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002681:China; D003062:Codon / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005838:Genotype; D015511:Goat Diseases / Q000235:genetics; D006041:Goats / Q000235:genetics*; D008297:Male; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics*; D012608:Scrapie / Q000235:genetics; D013045:Species Specificity
D003062:Codon; D017931:DNA Primers; D011328:Prions