Cloning and characterization of a human LYPD7, a new member of the Ly-6 superfamily.
Members of the Ly-6 (Lymphocyte Antigen 6) protein family share one or several repeat units of the LU domain that is defined by a distinct disulfide bonding pattern between 8 or 10 cysteine residues. Here we report the cloning and characterization of a novel human LU domain-containing gene, LYPD7 (LY6/PLAUR domain containing 7), isolated from human testis cDNA library, and mapped to 2q22.3-23.3 by searching the UCSC genomic database. The LYPD7 cDNA sequence consists of 1,600 nucleotides and contains an open reading frame of 624 bp, encoding a putative protein of 207 amino acid residues. RT-PCR analysis showed that LYPD7 was especially highly expressed in testis, lung, stomach, and prostate. Subcellular localization of LYPD7 demonstrated that the protein was localized in the cytoplasm when overexpressed in Hela cells. Furthermore, the subsequent analysis based on reporter gene assays suggested that overexpression of LYPD7 in HEK 293T cells was able to activate the transcriptional activities of AP1 (PMA).
Ni
Jun
J
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai 200433, PR China.
Lang
Qingyu
Q
Bai
Meirong
M
Zhong
Chaomin
C
Chen
Xinya
X
Wan
Bo
B
Yu
Long
L
D000595:Amino Acid Sequence; D000818:Animals; D000950:Antigens, Ly / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D002877:Chromosomes, Human / Q000235:genetics; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
D000950:Antigens, Ly; C540879:LYPD6B protein, human; D018808:Transcription Factor AP-1