Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer.
Parthenogenesis and somatic cell nuclear transfer (SCNT) are two methods for deriving embryonic stem (ES) cells that are genetically matched to the oocyte donor or somatic cell donor, respectively. Using genome-wide single nucleotide polymorphism (SNP) analysis, we demonstrate distinct signatures of genetic recombination that distinguish parthenogenetic ES cells from those generated by SCNT. We applied SNP analysis to the human ES cell line SCNT-hES-1, previously claimed to have been derived by SCNT, and present evidence that it represents a human parthenogenetic ES cell line. Genome-wide SNP analysis represents a means to validate the genetic provenance of an ES cell line.
Kim
Kitai
K
Division of Pediatric Hematology/Oncology, Children's Hospital Boston and Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Ng
Kitwa
K
Rugg-Gunn
Peter J
PJ
Shieh
Jae-Hung
JH
Kirak
Oktay
O
Jaenisch
Rudolf
R
Wakayama
Teruhiko
T
Moore
Malcolm A
MA
Pedersen
Roger A
RA
Daley
George Q
GQ
D000818:Animals; D002460:Cell Line; D020732:Cytogenetic Analysis; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000378:metabolism*; D015894:Genome, Human / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D051379:Mice; D053652:Nuclear Transfer Techniques; D010312:Parthenogenesis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011995:Recombination, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013048:Specimen Handling