title: Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification.
abstract: A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction.
authors: Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors: Hao-Tai
authors: HT
authors: Chen
authors: Jie
authors: J
authors: Zhang
authors: De-Hui
authors: DH
authors: Sun
authors: Yue-Feng
authors: YF
authors: Chu
authors: Xue-Peng
authors: XP
authors: Cai
authors: Xiang-Tao
authors: XT
authors: Liu
authors: Xue-Nong
authors: XN
authors: Luo
authors: Qing
authors: Q
authors: Liu
authors: Yong-Sheng
authors: YS
authors: Liu
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000175:diagnosis*; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D013696:Temperature; D013997:Time Factors
chemical_list: D017931:DNA Primers