Assays of MSCs with microarrays.
The rapid development of microarray technology during the last decade has greatly expanded the ability to define the genes expressed in cells. This chapter will focus on describing the steps required for conducting successful microarray experiments with multipotential stromal cells (MSCs). A complete microarray experiment, using the Affymetrix system, will be described starting from experimental design and ending with examples of data analysis using the dChip program.
Ylöstalo
Joni
J
Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA, USA.
Pochampally
Radhika
R
Prockop
Darwin J
DJ
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D017154:Stromal Cells / Q000166:cytology