title: Freshly isolated Langerhans cells negatively regulate naïve T cell activation in response to peptide antigen through cell-to-cell contact.
abstract: BACKGROUND
  Epidermal Langerhans cells (LCs) have been believed to function as professional antigen-presenting cells (APCs). However, LC-ablated mice reportedly suffer from severer contact hypersensitivity (CHS) upon cutaneous challenge with hapten than wild-type mice, suggesting LCs as regulators of adaptive immune responses in the skin.
  
  
  OBJECTIVE
  This study was designed to address the possible regulatory roles of LCs in the balanced primary adaptive immune responses to protein antigens.
  
  
  METHODS
  LCs were freshly isolated from skin of BALB/c mice (>95% positive for MHC class II). Naïve CD4+ T cells reactive to ovalbumin (OVA) were purified by FACS-sorting from lymph node cells of DO11.10 BALB/c mice, labeled with CSFE, and incubated with OVA peptide in the presence of splenic dendritic cells (DCs) and/or LCs. Cell division frequencies were determined by the degree of serially diluted expressions of CSFE in the individual CD4+ T cells.
  
  
  RESULTS
  Approximately 70% of them underwent cell division when naïve CD4+ T cells were activated by OVA presented by splenic DCs. In contrast, LCs only very modestly induced their cell division. Furthermore, LCs inhibited the cell division induced by splenic DCs, and this regulatory action was abolished by prevention of their contact to other cells, but not by the treatment with neutralizing antibodies against IL-10 or TGF-beta, well-established regulatory cytokines.
  
  
  CONCLUSIONS
  LCs negatively regulate the primary adaptive T cell response, presumably allowing well-controlled immune response in the skin.
authors: Department of Dermatology, Mie University School of Medicine, Tsu 514-8507, Japan.
authors: Yasutomo
authors: Y
authors: Imai
authors: Nobuki
authors: N
authors: Hayashi
authors: Koubun
authors: K
authors: Yasuda
authors: Hiroko
authors: H
authors: Tsutsui
authors: Hitoshi
authors: H
authors: Mizutani
authors: Kenji
authors: K
authors: Nakanishi
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D005260:Female; D005802:Genes, MHC Class II; D007801:Langerhans Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D012867:Skin / Q000166:cytology / Q000276:immunology*; D012871:Skin Diseases / Q000276:immunology
chemical_list: D010455:Peptides