Fluorimetric determination of cyclofenil using photochemical derivatization.
In this work, a fluorimetric approach for the determination of cyclofenil, 4-4'-(cyclohexylidenemethylene)bis(phenylacetate), is presented. The method was based on the intense fluorescence (250/410 nm) observed after a UV photochemical treatment of cyclofenil. The influence of the pH and solvent system and UV exposure time on the fluorescence magnitude was studied. Optimization of parameters was made using experimental design (factorial design and central composite design). Limit of detection (3S(b)/m) were estimated to be 1.1 x 10(-8)mol L(-1) with the linear dynamic range extending up to 8 x 10(-5)mol L(-1). This analytical approach was tested through the analysis of a commercial pharmaceutical formulation. In this case, tests enabled an average recovery of 98.3+/-3.9% (for n=9) using the analytical curve. The identification of the fluorescent derivative is proposed based on results achieved from GC-MS.
Pacheco
Wagner F
WF
Chemistry Department, Pontifícia Universidade Católica do Rio de Janeiro (PUC-RJ), Rio de Janeiro, RJ 22453-900, Brazil.
Batalha
João A L
JA
de Oliveira
Cláudia C
CC
Aucelio
Ricardo Q
RQ
D003506:Cyclofenil / Q000032:analysis*; D005300:Fertility Agents, Female; D005470:Fluorometry / Q000379:methods* / Q000592:standards*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations; D010777:Photochemistry / Q000379:methods; D012997:Solvents; D014466:Ultraviolet Rays
D005300:Fertility Agents, Female; D004364:Pharmaceutical Preparations; D012997:Solvents; D003506:Cyclofenil