Lithium thiazolidine-4-carboxylate: synthesis, spectroscopic characterization and preliminary in vitro cytotoxic studies in human HeLa cells.
A new water-soluble lithium salt of thiazolidine-4-carboxylic acid was synthesized and characterized by chemical and spectroscopic techniques. Elemental and mass spectrometric (ESI-MS) analyses of the solid compound fit to the composition LiC(4)H(6)NSO(2). (1)H, (13)C nuclear magnetic resonance (NMR), [(1)H-(15)N] NMR and infrared (IR) analyses permitted to elucidate the structure of the compound. Biological activity was evaluated by cytotoxic analysis using HeLa cells. Determination of cell death was assessed using a tetrazolium salt colorimetric assay, which reflects the cells viability.
Corbi
Pedro P
PP
Departamento de Ciências Biológicas e da Saúde, Centro Universitário de Araraquara-UNIARA, Associação São Bento de Ensino, Rua Voluntários da Pátria 1309, CEP 14801-320, Araraquara, SP, Brazil. pedrocorbi@yahoo.com
Andrade
Fabiana C
FC
Massabni
Antonio C
AC
Heinrich
Tassiele A
TA
Souza
Pedro P C
PP
Costa-Neto
Cláudio M
CM
D016923:Cell Death / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D018020:Lithium Compounds / Q000138:chemical synthesis* / Q000737:chemistry* / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D013055:Spectrophotometry, Infrared; D053778:Thiazolidines / Q000138:chemical synthesis* / Q000737:chemistry* / Q000633:toxicity
D018020:Lithium Compounds; D053778:Thiazolidines; C003438:thiazolidine-4-carboxylic acid