Microscale analysis of in vitro anaerobic degradation of lignocellulosic wastes by rumen microorganisms.
Anaerobic degradation of lignin in waste straw by ruminal microbes was directly observed using atomic force microscope (AFM). A series of high-resolution AFM images of the straw surface in the biodegradation show that the wax flakelets and lignin granules covering the straw surface were removed by the rumen microorganisms. Such degradation resulted in an exposure of cellulose fibers located inside the straw. The appearance of holes and microfibers in fermentation reveals that tunneling might be one of the ways for rumen microorganisms to attack the straw. Increases in the atomic ratio of oxygen to carbon (O/C) and the ratio C2/C3 in C1s spectra of X-ray photoelectron spectroscopy confirm that more cellulose was exposed on the surface after the anaerobic fermentation of straw. Gas chromatography/mass spectrometry analytical results demonstrate the decomposition of lignin by rumen microorganisms. Fourier transform infrared spectroscopy spectra and the measurement of degradation efficiency of the main straw components further verify these microscaled observations.
Hu
Zhen-Hu
ZH
Laboratory of Environmental Engineering, School of Chemistry, University of Science & Technology of China, Hefei 230026, China.
Liu
Shao-Yang
SY
Yue
Zheng-Bo
ZB
Yan
Li-Feng
LF
Yang
Ming-Tao
MT
Yu
Han-Qing
HQ
D000693:Anaerobiosis; D000818:Animals; D001673:Biodegradation, Environmental; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D008031:Lignin / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D012417:Rumen / Q000382:microbiology*; D013057:Spectrum Analysis / Q000379:methods; D014908:Triticum / Q000378:metabolism*; D014965:X-Rays
D008031:Lignin