Five donors-one recipient: modeling a mosaic of granulocytes, natural killer and T cells from cord-blood and third-party donors.
BACKGROUND
A 21-year-old man was admitted to hospital because of leukocytosis, thrombocytopenia and anemia. The patient had been in good health until a few days earlier, when he developed fever and night sweats and his performance status dramatically declined.


METHODS
Laboratory tests, immunophenotyping, cytogenetic analyses, bone-marrow biopsy, minimal residual disease analysis using quantitative real-time polymerase chain reaction, differential chimerism analysis using flow cytometry, mixed chimerism analysis, CT scans, electro-encephalography, cerebral magnetic resonance tomography.


METHODS
Bcr-abl-positive and Philadelphia-chromosome-positive acute lymphoblastic leukemia, and primary graft failure complicated by invasive fungal infection and cytomegalovirus encephalitis.


RESULTS
Double cord-blood rescue transplantation, third-party CD34-positive stem-cell rescue transplantation, third-party cytomegalovirus-specific T lymphocyte transplantation.
Schöttker
Björn
B
Department of Internal Medicine II, University of Würzburg, Germany.
Feuchtinger
Tobias
T
Schumm
Michael
M
Klinker
Erdwine
E
Handgretinger
Rupert
R
Einsele
Hermann
H
Stuhler
Gernot
G
D000328:Adult; D017690:Cell Transplantation; D036101:Cord Blood Stem Cell Transplantation; D006098:Granulocytes / Q000637:transplantation; D006801:Humans; D007694:Killer Cells, Natural / Q000637:transplantation; D008297:Male; D009030:Mosaicism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000628:therapy*; D013601:T-Lymphocytes / Q000637:transplantation