PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences.
The sequence diversity of groESL genes among Streptococcus bovis group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the groES and groEL sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between groES and groEL was variable in size (67-342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the groESL genes among species and subspecies ranged from 84.2 to 99.0 % in groES, and from 88.0 to 99.0 % in groEL. Based on the sequences determined, a Streptococcus bovis group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with AclI further differentiated the species and subspecies.
Chen
Hsiao-Jan
HJ
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
Tsai
Jui-Chang
JC
Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.|Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine, Taipei, Taiwan.
Chang
Tsung-Chain
TC
Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, Tainan, Taiwan.
Hung
Wei-Chun
WC
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
Tseng
Sung-Pin
SP
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
Hsueh
Po-Ren
PR
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.
Teng
Lee-Jene
LJ
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, Taiwan.|Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D018835:Chaperonin 10 / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D018833:Chaperonins / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology; D016985:Streptococcus bovis / Q000145:classification* / Q000235:genetics
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; C097970:GroESL protein, Bacteria; C066171:endodeoxyribonuclease AciI; D015252:Deoxyribonucleases, Type II Site-Specific; D018833:Chaperonins