Isolation of neural stem and precursor cells from rodent tissue.
Isolation and characterization of neural stem cells and lineage-specific progenitors provide important information for central nervous system development study and regenerative medicine. We describe methods for dissection of rodent embryonic spinal cords by enzymatic separation, and isolation and enrichment (or purification) of neuronal and glial precursors at different developing stages by fluorescence-activated cell sorting.
Wu
Yuanyuan
Y
Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.
Liu
Ying
Y
Chesnut
Jonathan D
JD
Rao
Mahendra S
MS
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D005260:Female; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*