Visualization of auxin gradients in embryogenesis.
Embryogenesis in Arabidopsis thaliana depends on the proper establishment and maintenance of local auxin accumulation. In the course of elucidating the connections between developmental progress and auxin distribution, several techniques have been developed to investigate spatial and temporal distribution of auxin response or accumulation in Arabidopsis embryos. This chapter reviews and describes two independent methods, the detection of the activity of auxin responsive transgenes and immunolocalization of auxin itself.
Sauer
Michael
M
ZMBP Developmental Genetics, University of TUbingen, Germany.
Friml
Jirí
J
D017360:Arabidopsis / Q000196:embryology*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D007150:Immunohistochemistry; D007210:Indoleacetic Acids / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics; D012639:Seeds / Q000502:physiology*
D007210:Indoleacetic Acids; D005966:Glucuronidase