title: [Immunization with catalase and UreB two-valence vaccine for preventing Helicobacter pylori infection in mice].
abstract: OBJECTIVE
  To investigate the effects of the two-valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and urease subunit UreB in preventing H. pyloriinfection in mice.
  
  
  METHODS
  C57BL/6 mice were divided into 7 groups and immunized with intragastric administration of catalase and UreB (both 100 microg) plus cholera toxin (CT, 2 microg), catalase (100 microg) plus CT (2 microg), UreB (100 microg) plus CT (2 microg), catalase (100 microg), UreB (100 microg), CT (2 microg), or PBS, respectively, once a week for 4 consecutive weeks. Two weeks after the last immunization, all the mice were challenged by live H. pylori, and sacrificed 4 weeks after the challenge to obtain the gastric mucosa samples for detecting H. pylori using semi-quantitative bacterial culture assay.
  
  
  RESULTS
  The total protection rate in mice immunized with the two-valence vaccine, single-valence vaccine of catalase, and single-valence vaccine of UreB was 83.3% (20/24), 41.7% (10/24) and 54.2% (13/24), respectively, and the rate in the other 4 groups were all 0. The H. pyloricolony density in mice with vaccination was significantly lower than that of other 4 groups (P<0.05). The total protection rate and H. pylori colony density differed significantly between the two-valence vaccination group and the single-valence vaccination groups (P<0.05).
  
  
  CONCLUSIONS
  The two-valence vaccine consisting of catalase, UreB and adjuvant has better immunoprotective effects than the single-valence vaccines.
authors: Department of Gastroenterology, People's Hospital of Guangdong Province, Guangzhou 510080, China.
authors: Huan-jian
authors: HJ
authors: Lin
authors: Qin
authors: Q
authors: Yang
authors: Jing
authors: J
authors: Li
authors: Ying
authors: Y
authors: Liu
authors: Qi-yi
authors: QY
authors: Wang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology*; D002374:Catalase / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000201:enzymology / Q000276:immunology*; D007114:Immunization / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011897:Random Allocation; D014510:Urease / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002374:Catalase; D014510:Urease