title: Long-term survival of HLA-DR15+ pig skin in SCID mice after reconstitution with human peripheral blood mononuclear cells and under short-term immunosuppression.
abstract: In allotransplantation, donor-recipient human leukocyte antigen (HLA) matches improve graft survival. For studies of the role of donor-recipient HLA II matching on xenotransplantation, we successfully generated HLA-DR15+ transgenic pigs the the skins of which were transplanted to SCID mice, which were thereafter reconstituted with HLA-DR15+ or -DR15(-) hPBMC. Cyclosporine was given intraperitoneally to SCID mice for 12 days. Human T cells were observed in SCID mice after reconstitution. Mixed lymphocytes responses showed greater responses by HLA-DR15(-) human peripheral blood mononuclear cells (hPBMC) against HLA-DR15+ porcine PBMC. HLA-DR15+ porcine skins survived more than 100 days in all SCID mice. HLA-DR15+ porcine skins were rejected in all non-SCID (Balb/c) mice. The histologic pictures of transplanted HLA-DR15+ porcine skins showed surviving porcine epithelium in remodeling murine dermis and little lymphocyte infiltration into the murine dermis. The long-term survival of HLA-DR15+ pig skin in all hPBMC-SCID mice might be due to poor engraftment or function of reconstituted T cells. Further studies are needed to clarify the role of donor-recipient matching of HLA-DR15.
authors: Department of Surgery, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan.
authors: H-C
authors: HC
authors: Tai
authors: C-F
authors: CF
authors: Tu
authors: J-M
authors: JM
authors: Lee
authors: L-L
authors: LL
authors: Ho
authors: Y-L
authors: YL
authors: Tseng
authors: N-K
authors: NK
authors: Chou
authors: T-S
authors: TS
authors: Yang
authors: C-N
authors: CN
authors: Weng
authors: P-H
authors: PH
authors: Lee
authors: K-J
authors: KJ
authors: Chang
authors: Y-B
authors: YB
authors: Tang
mesh_terms: D000818:Animals; D006085:Graft Survival / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D007165:Immunosuppression Therapy; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D016513:Mice, SCID; D012867:Skin / Q000276:immunology*; D016038:Skin Transplantation / Q000276:immunology*; D013552:Swine
chemical_list: D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C106559:HLA-DR15 antigen