Autoinducer AI-2 is involved in regulating a variety of cellular processes in Salmonella Typhimurium.
Salmonella Typhimurium is known to exhibit LuxS/AI-2-mediated cell signaling. We investigated the role of LuxS/AI-2 system on Salmonella Typhimurium protein expression using a proteomics approach based on two-dimensional gel electrophoresis (2DGE)-MALDI-MS. The global protein expression profiles of the wild-type, a luxS mutant, and a luxS mutant strain supplemented with AI-2 were compared. Seven proteins were differentially expressed when comparing the wild-type and luxS mutant strains, whereas 13 proteins were differentially expressed when comparisons were made between luxS mutant strains with and without AI-2 supplementation. The seven proteins that were differentially expressed between the wild-type and the luxS mutant strain were also differentially expressed in the luxS mutant strain supplemented with AI-2. The level of PhoP, a virulence determinant, was higher in the presence of AI-2. Proteins associated with the carbohydrate metabolism (pfkA, gpmI, and talB) and ATP synthesis (Pta gene product) were up-regulated by the presence of AI-2 molecules. These results provide experimental evidence that AI-2 molecules regulate a variety of cellular processes in Salmonella Typhimurium.
Soni
Kamlesh A
KA
Food Safety & Environmental Microbiology Program, Departments of Poultry Science, and Nutrition and Food Science, Texas A&M University, College Station, Texas, USA.
Jesudhasan
Palmy R
PR
Cepeda
Martha
M
Williams
Brad
B
Hume
Michael
M
Russell
William K
WK
Jayaraman
Arul
A
Pillai
Suresh D
SD
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D001681:Biological Assay; D050260:Carbohydrate Metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D007783:Lactones; D009154:Mutation; D040901:Proteomics; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014158:Transcription, Genetic; D015854:Up-Regulation; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria