title: HMGA2 mRNA expression correlates with the malignant phenotype in human thyroid neoplasias.
abstract: We have analysed the expression of the HMGA2 gene in a panel of normal and neoplastic thyroid tissues by immunohistochemistry and quantitative RT-PCR. HMGA2 protein was detectable in four out of 21 follicular carcinomas, 30 out of 45 papillary carcinomas, and 11 out of 12 undifferentiated carcinomas. As far as follicular thyroid adenomas are concerned, only three cases of the 31 analysed showed HMGA2 protein expression, whereas it was absent in seven normal thyroid tissues and in 12 hyperplastic nodules. Quantitative RT-PCR showed that almost all the papillary thyroid carcinomas and 13 out of 16 follicular thyroid carcinomas express much higher HMGA2 specific mRNA levels in comparison to normal thyroids and adenomas. Therefore, our data support the quantitative RT-PCR analysis of HMGA2 expression, rather than immunohistochemistry, as a powerful tool for the diagnosis of thyroid neoplasias.
authors: Istituto Nazionale dei Tumori, Fondazione Pascale, via Mariano Semmola, 80131 Naples, Italy.
authors: Gennaro
authors: G
authors: Chiappetta
authors: Angelo
authors: A
authors: Ferraro
authors: Emilia
authors: E
authors: Vuttariello
authors: Mario
authors: M
authors: Monaco
authors: Francesca
authors: F
authors: Galdiero
authors: Veronica
authors: V
authors: De Simone
authors: Daniela
authors: D
authors: Califano
authors: Pierlorenzo
authors: P
authors: Pallante
authors: Gerardo
authors: G
authors: Botti
authors: Luciano
authors: L
authors: Pezzullo
authors: Giovanna Maria
authors: GM
authors: Pierantoni
authors: Massimo
authors: M
authors: Santoro
authors: Alfredo
authors: A
authors: Fusco
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics*; D002291:Carcinoma, Papillary / Q000235:genetics*; D025743:HMGA2 Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D025743:HMGA2 Protein; D012333:RNA, Messenger; D012334:RNA, Neoplasm