title: The effect of geometry on three-dimensional tissue growth.
abstract: Tissue formation is determined by uncountable biochemical signals between cells; in addition, physical parameters have been shown to exhibit significant effects on the level of the single cell. Beyond the cell, however, there is still no quantitative understanding of how geometry affects tissue growth, which is of much significance for bone healing and tissue engineering. In this paper, it is shown that the local growth rate of tissue formed by osteoblasts is strongly influenced by the geometrical features of channels in an artificial three-dimensional matrix. Curvature-driven effects and mechanical forces within the tissue may explain the growth patterns as demonstrated by numerical simulation and confocal laser scanning microscopy. This implies that cells within the tissue surface are able to sense and react to radii of curvature much larger than the size of the cells themselves. This has important implications towards the understanding of bone remodelling and defect healing as well as towards scaffold design in bone tissue engineering.
authors: Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
authors: Monika
authors: M
authors: Rumpler
authors: Alexander
authors: A
authors: Woesz
authors: John W C
authors: JW
authors: Dunlop
authors: Joost T
authors: JT
authors: van Dongen
authors: Peter
authors: P
authors: Fratzl
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001846:Bone Development; D002460:Cell Line; D003198:Computer Simulation; D017886:Durapatite / Q000494:pharmacology*; D051379:Mice; D018613:Microscopy, Confocal; D010006:Osteoblasts / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite