Promoting the formation and stabilization of G-quadruplex by dinuclear RuII complex Ru2(obip)L4.
The remarkable ability of a new dinuclear Ru (II) complex Ru 2(obip)L 4 [obip = 2-(2-pyridyl)imidazo[4,5- f][1,10]-phenanthroline; L = 2,2'-bipyridine] to promote the formation and stabilization of the human telomeric repeat AG3(T2AG3)3 quadruplex was reported. The experimental results indicated that Ru 2(obip)L 4 could induce the formation of an antiparallel G-quadruplex structure in the absence of metal cations. It could induce positive T m shifts of +9.4 and +5.8 degrees C in Na (+) and K (+) buffers, respectively, in which an increase in the melting temperature of the quadruplex indicated a stabilizing effect. Binding stoichiometry with the quadruplex was investigated through a luminescence-based Job plot. The major inflection point for Ru 2(obip)L 4 at x = 0.48 was observed. The data were consistent with a 1:1 [quadruplex]/[complex] binding mode, which was suggestive of a specific Ru 2(obip)L 4-quadruplex interaction with a single guanine tetrad.
Shi
Shuo
S
Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Liu
Jie
J
Yao
Tianming
T
Geng
Xiaoting
X
Jiang
Lingfeng
L
Yang
Qingyuan
Q
Cheng
Lin
L
Ji
Liangnian
L
D015082:2,2'-Dipyridyl; D006801:Humans; D007093:Imidazoles; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010618:Phenanthrolines; D012428:Ruthenium; D016615:Telomere / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics
D007093:Imidazoles; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; D012428:Ruthenium