Human osteoblastic cell response to a Ca- and P-enriched titanium surface obtained by anodization.
The aim of the present study was to evaluate the in vitro osteogenic potential of subcultured human osteoblastic cells derived from alveolar bone on a titanium (Ti) surface produced by an anodized alkali treatment (BSP-AK). Primary osteoblastic cells were subcultured on BSP-AK and machined Ti discs (control) and grown for periods of up to 21 days under osteogenic conditions. Morphologic and biochemical methods were used to assess important parameters of in vitro bone-like tissue formation. Although no major differences were observed between the BSP-AK and the control Ti surface in terms of cell attachment and mineralized matrix formation, a significant increase in cell population, ALP activity, and collagen content was detected in cultures on BSP-AK surface. Our results demonstrate that human osteoblastic cells are sensitive to the BSP-AK-modified Ti surface during the transitional stage between the end of the proliferative phase and the onset of the differentiation/matrix maturation ones. Together with the good mechanical properties exhibited by the Ca- and P- coating, our findings suggest that BSP-AK treatment could be useful for the development of a new surface for dental and orthopedic implants.
Franco
Rosemeire de Lordo
Rde L
Cell Culture Laboratory, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Brazil.
Chiesa
Roberto
R
Beloti
Marcio Mateus
MM
de Oliveira
Paulo Tambasco
PT
Rosa
Adalberto Luiz
AL
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D010012:Osteogenesis; D010758:Phosphorus / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties; D014025:Titanium / Q000378:metabolism*
D020099:Coated Materials, Biocompatible; D010758:Phosphorus; D014025:Titanium; D000469:Alkaline Phosphatase; D002118:Calcium