Lysyl oxidase (LOX) mRNA expression and genes of the differentiated osteoblastic phenotype are upregulated in human osteosarcoma cells by suramin.
It is well known that suramin influences proliferation and differentiation of tumour cells. To study whether and how suramin effects osteosarcoma (OS) cells, proliferation, differentiation, LOX mRNA expression and telomerase activity (TA) was analysed in the human MG-63 and U-2 OS, and the rat UMR-106 OS cell lines. Data show that suramin inhibited proliferation in the human cell lines and upregulated alkaline phosphatase activity. TA was attenuated in the human cells while in UMR-106 it was not changed. In UMR-106 suramin had no influence on osteocalcin and LOX expression, in the human cells however, both genes were upregulated.
Buchinger
B
B
Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 4th Medical Department, Hanusch Hospital, Heinrich Collinstrasse 30, 1140 Vienna, Austria.
Spitzer
S
S
Karlic
H
H
Klaushofer
K
K
Varga
F
F
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D015675:Osteocalcin / Q000096:biosynthesis; D012516:Osteosarcoma; D008249:Protein-Lysine 6-Oxidase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D013498:Suramin / Q000494:pharmacology*; D019098:Telomerase / Q000378:metabolism; D015854:Up-Regulation
D016588:Anticarcinogenic Agents; D012333:RNA, Messenger; D015675:Osteocalcin; D013498:Suramin; D008249:Protein-Lysine 6-Oxidase; D019098:Telomerase; D000469:Alkaline Phosphatase