title: Whole-genome analysis of histone H3 lysine 4 and lysine 27 methylation in human embryonic stem cells.
abstract: We mapped Polycomb-associated H3K27 trimethylation (H3K27me3) and Trithorax-associated H3K4 trimethylation (H3K4me3) across the whole genome in human embryonic stem (ES) cells. The vast majority of H3K27me3 colocalized on genes modified with H3K4me3. These commodified genes displayed low expression levels and were enriched in developmental function. Another significant set of genes lacked both modifications and was also expressed at low levels in ES cells but was enriched for gene function in physiological responses rather than development. Commodified genes could change expression levels rapidly during differentiation, but so could a substantial number of genes in other modification categories. SOX2, POU5F1, and NANOG, pluripotency-associated genes, shifted from modification by H3K4me3 alone to colocalization of both modifications as they were repressed during differentiation. Our results demonstrate that H3K27me3 modifications change during early differentiation, both relieving existing repressive domains and imparting new ones, and that colocalization with H3K4me3 is not restricted to pluripotent cells.
authors: Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, WI 53706-1580, USA.
authors: Guangjin
authors: G
authors: Pan
authors: Shulan
authors: S
authors: Tian
authors: Jeff
authors: J
authors: Nie
authors: Chuhu
authors: C
authors: Yang
authors: Victor
authors: V
authors: Ruotti
authors: Hairong
authors: H
authors: Wei
authors: Gudrun A
authors: GA
authors: Jonsdottir
authors: Ron
authors: R
authors: Stewart
authors: James A
authors: JA
authors: Thomson
mesh_terms: D002454:Cell Differentiation; D019070:Cell Lineage; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D015894:Genome, Human / Q000235:genetics*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport
chemical_list: D006657:Histones; D008239:Lysine