title: Reconstruction of calvarial defect of rabbits using porous calcium silicate bioactive ceramics.
abstract: In this study, the in vivo bone-regenerative capacity and resorption of the porous beta-calcium silicate (beta-CaSiO(3), beta-CS) bioactive ceramics were investigated in a rabbit calvarial defect model, and the results were compared with porous beta-tricalcium phosphate (beta-Ca(3)(PO(4))(2), beta-TCP) bioceramics. The porous beta-CS and beta-TCP ceramics were implanted in rabbit calvarial defects and the specimens were harvested after 4, 8 and 16 weeks, and evaluated by Micro-CT and histomorphometric analysis. The Micro-CT and histomorphometric analysis showed that the resorption of beta-CS was much higher than that of beta-TCP. The TRAP-positive multinucleated cells were observed on the surface of beta-CS, suggesting a cell-mediated process involved in the degradation of beta-CS in vivo. The amount of newly formed bone was also measured and more bone formation was observed with beta-CS as compared with beta-TCP (p<0.05). Histological observation demonstrated that newly formed bone tissue grew into the porous beta-CS, and a bone-like apatite layer was identified between the bone tissue and beta-CS materials. The present studies showed that the porous beta-CS ceramics could stimulate bone regeneration and may be used as bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.
authors: Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
authors: Songfeng
authors: S
authors: Xu
authors: Kaili
authors: K
authors: Lin
authors: Zhen
authors: Z
authors: Wang
authors: Jiang
authors: J
authors: Chang
authors: Lin
authors: L
authors: Wang
authors: Jianxi
authors: J
authors: Lu
authors: Congqin
authors: C
authors: Ning
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D001862:Bone Resorption; D018786:Bone Substitutes / Q000592:standards; D017610:Calcium Compounds / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry; D002516:Ceramics; D019245:Compressive Strength; D004577:Electron Probe Microanalysis; D020007:Implants, Experimental; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D011817:Rabbits; D017640:Silicates / Q000737:chemistry*; D012886:Skull / Q000473:pathology / Q000601:surgery*; D014057:Tomography, X-Ray Computed
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D017610:Calcium Compounds; D002130:Calcium Phosphates; D017640:Silicates; C485817:beta-tricalcium phosphate; C031293:calcium silicate