Sulfated beta-glucan derived from oat bran with potent anti-HIV activity.
China is a major producer of oats; the annual harvested area of 350,000 ha yields approximately 465,000 tons, giving an average yield of 1.33 tons/ha. The bran is not used for animal feed as it is of poor digestibility and low nutritive content and is considered a waste byproduct. Therefore, it is advantageous to produce a value-added product from the bran. We extracted the native polysaccharide, a linear (1-3)-, (1-4)-linked beta-glucan (OBG) from the oat bran and synthesized a sulfated derivative OBGS containing 36.5% sulfate. OBGS had potent activity against a primary isolate of human immunodeficiency virus (HIV-1) in peripheral blood mononuclear cells at a concentration (EC(50)=5.98 x 10(-4) microM) approximately 15,000 times below its cytotoxic concentration. OBGS was also active postinfection (EC(50)=5.3 x 10(-4) microM) and protected pretreated peripheral mononuclear cells (EC(50)=5.2 x 10(-2) microM) washed free of the compounds prior to infection. Thus, OBGS has potential as a vaginal microbicide and is the first such report for oat bran derived sulfated beta-glucan.
Wang
Shun Chun
SC
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Zhangjiang Hi-Tech Park, Shanghai 201210, People's Republic of China.
Bligh
S W Annie
SW
Zhu
Chang Lian
CL
Shi
Song Shan
SS
Wang
Zheng Tao
ZT
Hu
Zhi Bi
ZB
Crowder
John
J
Branford-White
Christopher
C
Vella
Cherelyn
C
D019380:Anti-HIV Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D018554:Avena / Q000737:chemistry*; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D011134:Polysaccharides / Q000138:chemical synthesis* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D013431:Sulfates / Q000138:chemical synthesis* / Q000494:pharmacology; D047071:beta-Glucans / Q000737:chemistry*
D019380:Anti-HIV Agents; D011134:Polysaccharides; D013431:Sulfates; D047071:beta-Glucans; C067858:beta-glucan, (1-3)(1-4)-