Chemically modified oligonucleotides with efficient RNase H response.
Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.
Vester
Birte
B
Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
Boel
Anne Marie
AM
Lobedanz
Sune
S
Babu
B Ravindra
BR
Raunkjaer
Michael
M
Lindegaard
Dorthe
D
Raunak
Hrdlicka
Patrick J
PJ
Højland
Torben
T
Sharma
Pawan K
PK
Kumar
Surender
S
Nielsen
Poul
P
Wengel
Jesper
J
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016914:Ribonuclease H / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D016914:Ribonuclease H