title: An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes.
abstract: MIP-T3 is a human protein found previously to associate with microtubules and the kinesin-interacting neuronal protein DISC1 (Disrupted-in-Schizophrenia 1), but whose cellular function(s) remains unknown. Here we demonstrate that the C. elegans MIP-T3 ortholog DYF-11 is an intraflagellar transport (IFT) protein that plays a critical role in assembling functional kinesin motor-IFT particle complexes. We have cloned a loss of function dyf-11 mutant in which several key components of the IFT machinery, including Kinesin-II, as well as IFT subcomplex A and B proteins, fail to enter ciliary axonemes and/or mislocalize, resulting in compromised ciliary structures and sensory functions, and abnormal lipid accumulation. Analyses in different mutant backgrounds further suggest that DYF-11 functions as a novel component of IFT subcomplex B. Consistent with an evolutionarily conserved cilia-associated role, mammalian MIP-T3 localizes to basal bodies and cilia, and zebrafish mipt3 functions synergistically with the Bardet-Biedl syndrome protein Bbs4 to ensure proper gastrulation, a key cilium- and basal body-dependent developmental process. Our findings therefore implicate MIP-T3 in a previously unknown but critical role in cilium biogenesis and further highlight the emerging role of this organelle in vertebrate development.
authors: Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.
authors: Chunmei
authors: C
authors: Li
authors: Peter N
authors: PN
authors: Inglis
authors: Carmen C
authors: CC
authors: Leitch
authors: Evgeni
authors: E
authors: Efimenko
authors: Norann A
authors: NA
authors: Zaghloul
authors: Calvin A
authors: CA
authors: Mok
authors: Erica E
authors: EE
authors: Davis
authors: Nathan J
authors: NJ
authors: Bialas
authors: Michael P
authors: MP
authors: Healey
authors: Elise
authors: E
authors: Héon
authors: Mei
authors: M
authors: Zhen
authors: Peter
authors: P
authors: Swoboda
authors: Nicholas
authors: N
authors: Katsanis
authors: Michel R
authors: MR
authors: Leroux
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D001693:Biological Transport, Active; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002923:Cilia / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D005407:Flagella / Q000502:physiology*; D017238:Genes, Helminth; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D009024:Morphogenesis; D046912:Multiprotein Complexes; D009154:Mutation; D009475:Neurons, Afferent / Q000502:physiology; D010641:Phenotype; D015398:Signal Transduction
chemical_list: D029742:Caenorhabditis elegans Proteins; D017931:DNA Primers; D018509:DNA, Helminth; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; C412724:TRAF3IP1 protein, human