title: Pregnancy after trophectoderm biopsy of frozen-thawed blastocyst.
abstract: OBJECTIVE
  To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst.
  
  
  METHODS
  Techniques and instrumentation.
  
  
  METHODS
  A University Medical Center.
  
  
  METHODS
  Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program.
  
  
  METHODS
  Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance.
  
  
  METHODS
  Serum betahCG and transvaginal ultrasound.
  
  
  RESULTS
  Positive betahCG and ongoing pregnancy.
  
  
  CONCLUSIONS
  Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.
authors: Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Stanford University Medical Center, Stanford, California 94305, USA. rlathi@stanford.edu
authors: Ruth Bunker
authors: RB
authors: Lathi
authors: Barry
authors: B
authors: Behr
mesh_terms: D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000502:physiology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000097:blood; D002970:Cleavage Stage, Ovum / Q000473:pathology; D004624:Embryo Transfer; D005260:Female; D005615:Freezing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D014327:Trophoblasts / Q000166:cytology
chemical_list: D018997:Chorionic Gonadotropin, beta Subunit, Human