title: Gene activation using FLP recombinase in C. elegans.
abstract: The FLP enzyme catalyzes recombination between specific target sequences in DNA. Here we use FLP to temporally and spatially control gene expression in the nematode C. elegans. Transcription is blocked by the presence of an "off cassette" between the promoter and the coding region of the desired product. The "off cassette" is composed of a transcriptional terminator flanked by FLP recognition targets (FRT). This sequence can be excised by FLP recombinase to bring together the promoter and the coding region. We have introduced two fluorescent reporters into the system: a red reporter for promoter activity prior to FLP expression and a green reporter for expression of the gene of interest after FLP expression. The constructs are designed using the multisite Gateway system, so that promoters and coding regions can be quickly mixed and matched. We demonstrate that heat-shock-driven FLP recombinase adds temporal control on top of tissue specific expression provided by the transgene promoter. In addition, the temporal switch is permanent, rather than acute, as is usually the case for heat-shock driven transgenes. Finally, FLP expression can be driven by a tissue specific promoter to provide expression in a subset of cells that can only be addressed as the intersection of two available promoters. As a test of the system, we have driven the light chain of tetanus toxin, a protease that cleaves the synaptic vesicle protein synaptobrevin. We show that we can use this to inactivate synaptic transmission in all neurons or a subset of neurons in a FLP-dependent manner.
authors: Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, United States of America.
authors: M Wayne
authors: MW
authors: Davis
authors: J Jason
authors: JJ
authors: Morton
authors: Dana
authors: D
authors: Carroll
authors: Erik M
authors: EM
authors: Jorgensen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation; D017238:Genes, Helminth; D049452:Green Fluorescent Proteins / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011995:Recombination, Genetic; D013744:Tetanus Toxin / Q000235:genetics; D015533:Transcriptional Activation; D000097573:Red Fluorescent Protein
chemical_list: D004254:DNA Nucleotidyltransferases; D017931:DNA Primers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin; D000097573:Red Fluorescent Protein; C045119:FLP recombinase