Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor.
Endothelial exocytosis of granules is a rapid response to vascular injury. However, the molecular machinery that regulates exocytosis in endothelial cells is not well understood. Recently developed techniques have defined the endothelial proteins that control vesicle and granule trafficking in endothelial cells. These techniques have revealed that syntaxin 4, synaptobrevin 3, and N-ethylmaleimide-sensitive factor (NSF) play a critical role in endothelial granule exocytosis. Additional studies have shown that nitric oxide regulates exocytosis by chemically modifying NSF. Further characterization of the factors that regulate exocytosis will lead to novel treatments for vascular diseases such as myocardial infarction and stroke.
Yamakuchi
Munekazu
M
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ferlito
Marcella
M
Morrell
Craig N
CN
Matsushita
Kenji
K
Fletcher
Craig A
CA
Cao
Wangsen
W
Lowenstein
Charles J
CJ
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005089:Exocytosis; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D047468:Immunoprecipitation; D036904:Leukocyte Rolling; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D018715:Microscopy, Video; D052066:N-Ethylmaleimide-Sensitive Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D050765:Qa-SNARE Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D022142:Secretory Vesicles / Q000378:metabolism*; D013997:Time Factors; D051040:Vesicle-Associated Membrane Protein 3 / Q000378:metabolism; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
D010446:Peptide Fragments; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins; D051040:Vesicle-Associated Membrane Protein 3; D054322:tat Gene Products, Human Immunodeficiency Virus; D009569:Nitric Oxide; D052066:N-Ethylmaleimide-Sensitive Proteins