title: Monolithic porous polymer stationary phases in polyimide chips for the fast high-performance liquid chromatography separation of proteins and peptides.
abstract: Poly(lauryl methacrylate-co-ethylene dimethacrylate) and poly(styrene-co-divinylbenzene) stationary phases in monolithic format have been prepared by thermally initiated free radical polymerization within polyimide chips featuring channels having a cross-section of 200micromx200microm and a length of 6.8cm. These chips were then used for the separation of a mixture of proteins including ribonuclease A, myoglobin, cytochrome c, and ovalbumin, as well as peptides. The separations were monitored by UV adsorption. Both the monolithic phases based on methacrylate and on styrene chemistries enabled the rapid baseline separation of most of the test mixtures. Best performance was achieved with the styrenic monolith leading to fast baseline separation of all four proteins in less than 2.5min. The in situ monolith preparation process affords microfluidic devices exhibiting good batch-to-batch and injection-to-injection repeatability.
authors: College of Chemistry, University of California, Berkeley, CA 94720-1460, USA.
authors: Pavel A
authors: PA
authors: Levkin
authors: Sebastiaan
authors: S
authors: Eeltink
authors: Thomas R
authors: TR
authors: Stratton
authors: Reid
authors: R
authors: Brennen
authors: Karla
authors: K
authors: Robotti
authors: Hongfeng
authors: H
authors: Yin
authors: Kevin
authors: K
authors: Killeen
authors: Frantisek
authors: F
authors: Svec
authors: Jean M J
authors: JM
authors: Fréchet
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007094:Imides / Q000737:chemistry*; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D007094:Imides; D010455:Peptides; D011506:Proteins