Elimination kinetic of 17beta-estradiol 3-benzoate and 17beta-nandrolone laureate ester metabolites in calves' urine.
Efficient control of the illegal use of anabolic steroids must both take into account metabolic patterns and associated kinetics of elimination; in this context, an extensive animal experiment involving 24 calves and consisting of three administrations of 17beta-estradiol 3-benzoate and 17beta-nandrolone laureate esters was carried out over 50 days. Urine samples were regularly collected during the experiment from all treated and non-treated calves. For sample preparation, a single step high throughput protocol based on 96-well C(18) SPE was developed and validated according to the European Decision 2002/657/EC requirements. Decision limits (CCalpha) for steroids were below 0.1 microg L(-1), except for 19-norandrosterone (CCalpha=0.7 microg L(-1)) and estrone (CCalpha=0.3 microg L(-1)). Kinetics of elimination of the administered 17beta-estradiol 3-benzoate and 17beta-nandrolone laureate were established by monitoring 17beta-estradiol, 17alpha-estradiol, estrone and 17beta-nandrolone, 17alpha-nandrolone, 19-noretiocholanolone, 19-norandrostenedione, respectively. All animals demonstrated homogeneous patterns of elimination both from a qualitative (metabolite profile) and quantitative point of view (elimination kinetics in urine). Most abundant metabolites were 17alpha-estradiol and 17alpha-nandrolone (>20 and 2 mg L(-1), respectively after 17beta-estradiol 3-benzoate and 17beta-nandrolone laureate administration) whereas 17beta-estradiol, estrone, 17beta-nandrolone, 19-noretiocholanolone and 19-norandrostenedione were found as secondary metabolites at concentration values up to the microg L(-1) level. No significant difference was observed between male and female animals. The effect of several consecutive injections on elimination profiles was studied and revealed a tendency toward a decrease in the biotransformation of administered steroid 17beta form.
Pinel
Gaud
G
Laboratoire d'Etude des Résidus et Contaminants dans les Aliments, Ecole Nationale Vétérinaire de Nantes, Route de Gachet, BP 50707, 44307 Nantes cedex 3, France. laberca@vet-nantes.fr
Rambaud
Lauriane
L
Cacciatore
Giuseppe
G
Bergwerff
Aldert
A
Elliott
Chris
C
Nielen
Michel
M
Le Bizec
Bruno
B
D045930:Anabolic Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine; D000735:Androstenedione / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000652:urine; D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000652:urine; D004962:Estranes / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine; D004970:Estrone / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine; D005260:Female; D007700:Kinetics; D008297:Male; D008657:Metabolic Clearance Rate; D009277:Nandrolone / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000652:urine*
D045930:Anabolic Agents; D004962:Estranes; C074283:estradiol 3-benzoate; D004970:Estrone; D000735:Androstenedione; D004958:Estradiol; C036015:19-norandrosterone; D009277:Nandrolone; C002828:19-norandrostenedione