Analysis of geminivirus DNA replication by 2-D gel.
The technique described was developed for the separation of begomovirus DNA. DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots. The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.
Saunders
Keith
K
Biological Chemistry, John Innes Centre, Norwich, NR4 7UH, UK.
D004261:DNA Replication; D018744:DNA, Plant / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D017791:Geminiviridae / Q000235:genetics*; D016679:Genome, Viral; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D010942:Plant Viruses / Q000235:genetics; D010944:Plants / Q000821:virology*
D018744:DNA, Plant; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D007202:Indicators and Reagents