Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.
We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.
Zhang
Changqing
C
Department of Plant Sciences , The University of Arizona, Tucson, AZ 85721, USA.
Barthelson
Roger A
RA
Lambert
Georgina M
GM
Galbraith
David W
DW
D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D052585:Phloem / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism
D049452:Green Fluorescent Proteins