Detecting mitochondrial RNA and other cellular events in living cells.
Intracellular signaling can be monitored in vivo in living cells by genetically encoded intracellular fluorescent probes. In this review, three aspects of these probes are introduced: 1) the imaging dynamics of endogenous mitochondrial RNA; 2) nuclear receptor and coactivator/corepressor interactions, and; 3) the signal sequence in mitochondrial intermembrane space. These probes are generally applicable to fundamental biological studies as well as for assaying and screening possible pharmaceutical or toxic chemicals that facilitate or inhibit cellular signaling pathways.
Umezawa
Yoshio
Y
Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. umezawa@chem.s.u-tokyo.ac.jp
D015374:Biosensing Techniques / Q000379:methods*; D002477:Cells / Q000166:cytology / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D051336:Mitochondrial Membranes / Q000737:chemistry / Q000378:metabolism; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D000077278:RNA, Mitochondrial; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D014116:Toxicology / Q000379:methods*
D005456:Fluorescent Dyes; D004364:Pharmaceutical Preparations; D000077278:RNA, Mitochondrial; D018160:Receptors, Cytoplasmic and Nuclear; D012313:RNA