title: Oxygen diffusion through natural extracellular matrices: implications for estimating "critical thickness" values in tendon tissue engineering.
abstract: Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (Alloderm), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7 x 10(-6) - 2 x 10(-5) cm2/s, Alloderm: 1.9 - 3.1 x 10(-5) cm2/s, and canine fascia lata: 1.6 - 4 x 10(-5) cm2/s. We used the experimental oxygen diffusivity data for these natural ECMs in a mathematical model of oxygen diffusion through a cell-seeded scaffold to estimate the critical size of cell-seeded scaffold that can be cultured in vitro.
authors: Department of Biomedical Engineering, The Cleveland Clinic, Cleveland, Ohio 44195, USA.
authors: Caroline
authors: C
authors: Androjna
authors: Jorge E
authors: JE
authors: Gatica
authors: Joanne M
authors: JM
authors: Belovich
authors: Kathleen A
authors: KA
authors: Derwin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D019149:Bioreactors; D003094:Collagen / Q000378:metabolism; D004058:Diffusion; D004285:Dogs; D005109:Extracellular Matrix / Q000378:metabolism*; D005206:Fascia Lata / Q000378:metabolism / Q000637:transplantation; D006801:Humans; D007413:Intestinal Mucosa / Q000637:transplantation; D007421:Intestine, Small / Q000637:transplantation; D008962:Models, Theoretical; D010100:Oxygen / Q000378:metabolism*; D034421:Sus scrofa; D013710:Tendons; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: C440568:Alloderm; D001672:Biocompatible Materials; D003094:Collagen; D010100:Oxygen