Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor.
This paper describes the combination of electrochemical immunosensor using gold nanoparticles (GNPs)/carbon nanotubes (CNTs) hybrids platform with horseradish peroxidase (HRP)-functionalized gold nanoparticle label for the sensitive detection of human IgG (HIgG) as a model protein. The GNPs/CNTs nanohybrids covered on the glass carbon electrode (GCE) constructed an effective antibody immobilization matrix and made the immobilized biomolecules hold high stability and bioactivity. Enhanced sensitivity was obtained by using bioconjugates featuring HRP labels and secondary antibodies (Ab(2)) linked to GNPs at high HRP/Ab(2) molar ratio. The approach provided a linear response range between 0.125 and 80ng/mL with a detection limit of 40pg/mL. The immunosensor showed good precision, acceptable stability and reproducibility and could be used for the detection of HIgG in real samples, which provided a potential alternative tool for the detection of protein in clinical laboratory.
Cui
Rongjing
R
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Huang
Haiping
H
Yin
Zhengzhi
Z
Gao
Di
D
Zhu
Jun-Jie
JJ
D015374:Biosensing Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*
D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D006046:Gold; D006735:Horseradish Peroxidase