Separation of complex branched polymers by size-exclusion chromatography probed with multiple detection.
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibration principle). Molecular weights can be determined using viscometry (relying on universal calibration) and light scattering (independent of universal calibration). In the case of complex branched polyacrylates with tetrahydrofuran as eluent, universal calibration is valid, although the separation in term of molecular weight is incomplete: a given elution slice contains a range of molecular weights, described in terms of a 'local polydispersity'. The local polydispersity index decreases when the number of branches per chain increases and complete separation is reached for highly branched chains.
Gaborieau
Marianne
M
Centre for Nutrition and Food Sciences, Hartley Teakle Building, The University of Queensland, Brisbane, QLD 4072, Australia.
Nicolas
Julien
J
Save
Maud
M
Charleux
Bernadette
B
Vairon
Jean-Pierre
JP
Gilbert
Robert G
RG
Castignolles
Patrice
P
D002850:Chromatography, Gel / Q000379:methods*; D008970:Molecular Weight; D011108:Polymers / Q000302:isolation & purification*
D011108:Polymers