title: Virus-induced gene silencing as a tool to identify host genes affecting viral pathogenicity.
abstract: Host factors are crucial determinants of viral pathogenicity. Identifying host factors and their contributions to virus infections may lead to the development of novel antiviral strategies. The recently developed virus-induced gene silencing (VIGS) approach offers a rapid means to knock down expression of a given gene in plants. VIGS can be used to determine biological function of candidate genes or to discover new genes that play a role in a given biological pathway. Here, we describe genome-wide Tobacco rattle virus (TRV)-based VIGS screening methods to identify host factors involved in viral pathogenicity.
authors: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.
authors: Xiaohong
authors: X
authors: Zhu
authors: S P
authors: SP
authors: Dinesh-Kumar
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D020868:Gene Silencing; D010935:Plant Diseases / Q000382:microbiology / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity; D012639:Seeds / Q000821:virology; D014026:Nicotiana / Q000821:virology; D017898:Tobamovirus / Q000235:genetics*