title: Circulating endothelial progenitor cells as a new marker of endothelial dysfunction or repair in acute stroke.
abstract: OBJECTIVE
  Understanding on distinct subsets of endothelial progenitor cells may provide insights of endothelial dysfunction or repair in the acute ischemic event. Recent in vitro data have reported the colony-forming unit (CFU) and outgrowth cell population as a subset of endothelial progenitor cells. In this study, we undertook to validate the significance of CFU number and outgrowth cell yield in acute stroke.
  
  
  METHODS
  Mononuclear cells were isolated from the peripheral blood of 75 patients with acute stroke, 45 patients with chronic stroke, and 40 age-matched healthy volunteers. CFU numbers were counted after culturing them for 7 days, and outgrowth cell appearance was measured during the 2 months of culture. Endothelial progenitor cell function was also evaluated by matrigel plate assays. Independent parameters predicting CFU number and outgrowth cell yield were assessed using logistic regression analysis.
  
  
  RESULTS
  The CFU numbers and tube formation abilities in matrigel assays were significantly reduced in patients with acute stroke compared with patients with chronic stroke or healthy control subjects. Moreover, patients with large artery atherosclerosis had much lower CFU numbers and functional activities than ones with cardioembolism. Outgrowth cells were isolated from 10% of healthy control subjects and 22% of patients with chronic stroke during the cultures, but from 71% of patients with stroke. Multivariate analysis identified glycosylated hemoglobin and National Institutes of Health Stroke Scale on admission as significant independent predictors of a low CFU number and a high isolation frequency of outgrowth cells, respectively.
  
  
  CONCLUSIONS
  CFU number may thus represent an accumulated endothelial progenitor cell dysfunctional status, whereas outgrowth cell appearance may reflect the resilience of the systemic circulation to acute ischemic stress.
authors: Stem Cell Research Center, Department of Neurology, Seoul National University Hospital, Neuroscience Research Institute of SNUMRC, Seoul National University, Seoul, Korea.
authors: Kon
authors: K
authors: Chu
authors: Keun-Hwa
authors: KH
authors: Jung
authors: Soon-Tae
authors: ST
authors: Lee
authors: Hee-Kwon
authors: HK
authors: Park
authors: Dong-In
authors: DI
authors: Sinn
authors: Jeong-Min
authors: JM
authors: Kim
authors: Dong-Hyun
authors: DH
authors: Kim
authors: Jin-Hee
authors: JH
authors: Kim
authors: Se-Jeong
authors: SJ
authors: Kim
authors: Eun-Cheol
authors: EC
authors: Song
authors: Manho
authors: M
authors: Kim
authors: Sang Kun
authors: SK
authors: Lee
authors: Jae-Kyu
authors: JK
authors: Roh
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis; D002545:Brain Ischemia / Q000175:diagnosis / Q000503:physiopathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002536:Cerebral Arteries / Q000166:cytology* / Q000503:physiopathology; D003094:Collagen; D003114:Colony-Forming Units Assay / Q000379:methods; D004338:Drug Combinations; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D006801:Humans; D007797:Laminin; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000502:physiology; D011237:Predictive Value of Tests; D011379:Prognosis; D011509:Proteoglycans; D012016:Reference Values; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology; D020521:Stroke / Q000175:diagnosis / Q000503:physiopathology*
chemical_list: D015415:Biomarkers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen