Enhanced migration and CXCR4 over-expression in fibroblasts with telomerase reconstitution.
Telomerase reconstitution shows great potential for cell treatment and tissue engineering. Although the effects of telomerase on cell lifespan are well documented, the effects of telomerase on cellular biological characteristics, such as cellular migration, are relatively unknown. In this study, we tried to investigate if telomerase is involved in the regulation of fibroblast migration and the mechanism behind it. We found that when stimulated with a chemokine, CXCL12, the rate of migration was significantly higher in fibroblasts with telomerase reconstitution than that in fibroblasts without. Furthermore, the CXCL12 receptor, CXCR4, and multiple down-stream factors (Rho family members), were upregulated in the telomerase reconstituted fibroblasts. We concluded for the first time that telomerase reconstitution increased fibroblast migration through activation of CXCL12/CXCR4 axis and Rho family. The finding that fibroblasts with telomerase reconstitution have enhanced migration may have broad implications for cell therapy.
Qu
Yi
Y
Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Mao
Meng
M
Li
Xihong
X
Zhang
Lin
L
Huang
Xiang
X
Yang
Chunlei
C
Zhao
Fengyang
F
Xiong
Ying
Y
Mu
Dezhi
D
D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000494:pharmacology; D024042:Collagen Type I / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D051265:NF-E2-Related Factor 1 / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*; D014746:Vimentin / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000378:metabolism
D054377:Chemokine CXCL12; D024042:Collagen Type I; D051265:NF-E2-Related Factor 1; C088357:NFE2L1 protein, human; D012333:RNA, Messenger; D019718:Receptors, CXCR4; D014746:Vimentin; D019098:Telomerase; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins; D020741:rho GTP-Binding Proteins