title: Autophagic machinery activated by dengue virus enhances virus replication.
abstract: Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication.
authors: Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors: Ying-Ray
authors: YR
authors: Lee
authors: Huan-Yao
authors: HY
authors: Lei
authors: Ming-Tao
authors: MT
authors: Liu
authors: Jen-Ren
authors: JR
authors: Wang
authors: Shun-Hua
authors: SH
authors: Chen
authors: Ya-Fen
authors: YF
authors: Jiang-Shieh
authors: Yee-Shin
authors: YS
authors: Lin
authors: Trai-Ming
authors: TM
authors: Yeh
authors: Ching-Chuan
authors: CC
authors: Liu
authors: Hsiao-Sheng
authors: HS
authors: Liu
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D000071187:Autophagy-Related Protein 5; D002460:Cell Line; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D006224:Cricetinae; D003716:Dengue Virus / Q000472:pathogenicity* / Q000502:physiology; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010588:Phagosomes / Q000648:ultrastructure; D014779:Virus Replication / Q000502:physiology*; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D000071187:Autophagy-Related Protein 5; D051907:Lysosomal Membrane Proteins; D008869:Microtubule-Associated Proteins; C502778:ATG5 protein, human; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; C477012:MAP1LC3A protein, human