The effect of signal suppression and mobile phase composition on the simultaneous analysis of multiple classes of acidic/neutral pharmaceuticals and personal care products in surface water by solid-phase extraction and ultra performance liquid chromatography-negative electrospray tandem mass spectrometry.
A new multi-residue method for the determination of 25 acidic/neutral pharmaceuticals (antibiotics, anti-inflammatory/analgesics, lipid regulating agents, diuretics, triazides, H2-receptor antagonists, cardiac glicozides and angiotensin II antagonists) and personal care products (sunscreen agents and preservatives) in surface water with the usage of a new technique: ultra performance liquid chromatography-negative electrospray tandem mass spectrometry (UPLC-MS/MS) was developed and validated. The novel UPLC system with 1.7 microm particle-packed column allowed for good resolution of analytes with the application of low mobile phase flow rates (0.05 mL min(-1)) and short retention times (from 4.7 min to 13.3 min) delivering a fast and cost-effective multi-residue method. SPE with the usage of Oasis MCX strong cation-exchange mixed-mode polymeric sorbent was chosen for sample clean-up and concentration. The influence of mobile-phase composition, matrix assisted ion suppression and SPE recovery on the sensitivity of the method was identified and quantified. The instrumental limits of quantification varied from 0.2 microgL(-1) to 30mugL(-1). The method limits of quantification were at low nanogram per litre levels and ranged from 0.3 ng L(-1) to 30 ng L(-1). The instrumental and method intra-day and inter-day repeatabilities were on average less than 5%. The method was successfully applied for the determination of PPCPs in River Taff. Thirteen compounds were determined in river water at levels ranging from a single to a few hundred nanograms per litre. Among them were ten pharmaceuticals (aspirin, salicylic acid, ketoprofen, naproxen, diclofenac, ibuprofen, mefenamic acid, furosemide, sulfasalazine and valsartan) and three personal care products (methyl- and ethylparaben and 4-benzophenone).
Kasprzyk-Hordern
Barbara
B
University of Glamorgan, Sustainable Environment Research Centre, Pontypridd CF37 1DL, UK.
Dinsdale
Richard M
RM
Guwy
Alan J
AJ
D002853:Chromatography, Liquid / Q000379:methods; D005618:Fresh Water; D004364:Pharmaceutical Preparations / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical