Increased uncoupling protein 1 mRNA expression in mice brown adipose tissue after burn injury.
Brown adipose tissue (BAT) contains numerous mitochondria and is characterized by the presence of uncoupling protein 1 (UCP1). UCP1 is the main mediator of thermogenesis that plays an important role in the modulation of energy balance. The authors hypothesize that alterations in the expression of UCP1 might be involved in the major metabolic disorders occurring during burn trauma. The present study is designed to explore the potential role of the UCP1 in metabolic disorders after burn injury. The authors have used the real-time reverse transcription-polymerase chain reaction to quantify UCP1 mRNA expression in the mice BAT and white adipose tissue (WAT). UCP1 mRNA expression was up-regulated in BAT, especially at 24 hours after burn. UCP1 mRNA expression was detectable and also up-regulated by burn injury in WAT. The authors provide evidence that one of the mechanisms mediating hypermetabolism and increased energy expenditure in burn injury is a pronounced increase in thermogenic capacity, as illustrated by robust gene expression of UCP1 in BAT and WAT.
Zhang
Qin
Q
Department of Surgery, Massachusetts General Hospital, and Shriners Hospital for Children, Boston, MA 02114, USA.
Ma
Bangyi
B
Fischman
Alan J
AJ
Tompkins
Ronald G
RG
Carter
Edward A
EA
D002001:Adipose Tissue, Brown / Q000503:physiopathology*; D052436:Adipose Tissue, White / Q000503:physiopathology*; D000818:Animals; D002056:Burns / Q000150:complications* / Q000235:genetics / Q000503:physiopathology; D004734:Energy Metabolism; D015870:Gene Expression; D007473:Ion Channels / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D024101:Mitochondrial Proteins / Q000096:biosynthesis / Q000235:genetics*; D023421:Models, Animal; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1
D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1