Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids.
Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.
Vargas
Marisol
M
Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, (CIB, CSIC) Campus de la Ciudad Universitaria, Av, Ramiro de Maeztu 9, 28040 Madrid, Spain. marisolvargasco@yahoo.com
Martínez-García
Belén
B
Díaz-Ruíz
José Ramón
JR
Tenllado
Francisco
F
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000818:Animals; D001042:Aphids / Q000821:virology*; D020029:Base Pairing; D003001:Cloning, Molecular; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified / Q000382:microbiology / Q000821:virology; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D014026:Nicotiana / Q000382:microbiology / Q000821:virology
D012330:RNA, Double-Stranded; D012367:RNA, Viral