title: Triple gene block protein interactions involved in movement of Barley stripe mosaic virus.
abstract: Barley stripe mosaic virus (BSMV) encodes three movement proteins in an overlapping triple gene block (TGB), but little is known about the physical interactions of these proteins. We have characterized a ribonucleoprotein (RNP) complex consisting of the TGB1 protein and plus-sense BSMV RNAs from infected barley plants and have identified TGB1 complexes in planta and in vitro. Homologous TGB1 binding was disrupted by site-specific mutations in each of the first two N-terminal helicase motifs but not by mutations in two C-terminal helicase motifs. The TGB2 and TGB3 proteins were not detected in the RNP, but affinity chromatography and yeast two-hybrid experiments demonstrated that TGB1 binds to TGB3 and that TGB2 and TGB3 form heterologous interactions. These interactions required the TGB2 glycine 40 and the TGB3 isoleucine 108 residues, and BSMV mutants containing these amino acid substitution were unable to move from cell to cell. Infectivity experiments indicated that TGB1 separated on a different genomic RNA from TGB2 and TGB3 could function in limited cell-to-cell movement but that the rates of movement depended on the levels of expression of the proteins and the contexts in which they are expressed. Moreover, elevated expression of the wild-type TGB3 protein interfered with cell-to-cell movement but movement was not affected by the similar expression of a TGB3 mutant that fails to interact with TGB2. These experiments suggest that BSMV movement requires physical interactions of TGB2 and TGB3 and that substantial deviation from the TGB protein ratios expressed by the wild-type virus compromises movement.
authors: Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
authors: Hyoun-Sub
authors: HS
authors: Lim
authors: Jennifer N
authors: JN
authors: Bragg
authors: Uma
authors: U
authors: Ganesan
authors: Diane M
authors: DM
authors: Lawrence
authors: Jialin
authors: J
authors: Yu
authors: Masimachi
authors: M
authors: Isogai
authors: John
authors: J
authors: Hammond
authors: Andrew O
authors: AO
authors: Jackson
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D002846:Chromatography, Affinity; D001467:Hordeum / Q000821:virology*; D016297:Mutagenesis, Site-Directed; D009698:Nucleoproteins / Q000737:chemistry / Q000302:isolation & purification; D053830:Plant Viral Movement Proteins / Q000378:metabolism*; D010942:Plant Viruses / Q000502:physiology*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012328:RNA Viruses / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D009698:Nucleoproteins; D053830:Plant Viral Movement Proteins