title: Interference by atrazine and bisphenol-A with progestin binding to the ovarian progestin membrane receptor and induction of oocyte maturation in Atlantic croaker.
abstract: Recent studies have shown that several environmental chemicals which disrupt classical genomic steroid actions can also interfere with nongenomic steroid actions initiated at the cell surface. The effects of bisphenol-A and atrazine on the nongenomic actions of a progestin, 17,20 beta,21-trihydroxy-4-pregnen-3-one (20 beta-S), on oocyte maturation (OM) were investigated an in vitro Atlantic croaker (Micropogonias undulatus) oocyte bioassay. Treatment of croaker follicle-enclosed oocytes with either bisphenol-A or atrazine blocked OM in response to 20 beta-S in a concentration-dependent manner at 10-25 microM (2.2-5.7 ppm) and higher concentrations. These compounds were also effective competitors at concentrations of 10(-6)-10(-5)M of [3H]-20 beta-S binding to the ovarian progestin membrane receptor that mediates the OM response to 20 beta-S. The results suggest that diverse classes of environmental chemicals can disrupt nongenomic progestin actions via receptor-mediated mechanisms.
authors: University of Texas at Austin, Marine Science Institute, Port Aransas, TX 78373, USA. thomas@utmsi.utexas.edu
authors: Peter
authors: P
authors: Thomas
authors: Jennifer
authors: J
authors: Sweatman
mesh_terms: D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D001559:Benzhydryl Compounds; D003350:Cortodoxone / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010473:Perciformes / Q000502:physiology*; D010636:Phenols / Q000633:toxicity*; D011372:Progestins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011980:Receptors, Progesterone / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D000098826:Bisphenol A Compounds
chemical_list: D001280:Atrazine; D001559:Benzhydryl Compounds; D003350:Cortodoxone; D010636:Phenols; D011372:Progestins; D011980:Receptors, Progesterone; D014874:Water Pollutants, Chemical; C051954:17,20,21-trihydroxy-4-pregnen-3-one; C006780:bisphenol A; D000098826:Bisphenol A Compounds