title: DNA microarray mediated transcriptional profiling of Nitrosomonas europaea in response to linear alkylbenzene sulfonates.
abstract: Linear alkylbenzene sulfonates (LAS) constitute, quantitatively, the most important group of synthetic surfactants used today. We studied the gene expression of Nitrosomonas europaea in response to LAS using a DNA microarray because ammonia-oxidizers are thought to be more sensitive to LAS than other microorganisms. Our objective was to elucidate which genes are expressed for N. europaea in response to LAS exposure. Microarray analysis and real-time PCR assay revealed that c. 30 genes were significantly expressed after LAS exposure, in particular genes associated with energy production and conversion. Our findings demonstrate that physical disruption of membrane structures, which contain enzymes associated with energy production and conversion, might be an important explanation for the high sensitivity of N. europaea to LAS exposure.
authors: Ocean Research Institute, The University of Tokyo, Nakano, Tokyo, Japan. urakawa@ori.u-tokyo.ac.jp
authors: Hidetoshi
authors: H
authors: Urakawa
authors: Junpei
authors: J
authors: Matsumoto
authors: Kazuho
authors: K
authors: Inaba
authors: Satoshi
authors: S
authors: Tsuneda
mesh_terms: D017738:Alkanesulfonic Acids / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods*; D043370:Nitrosomonas europaea / Q000187:drug effects* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D017738:Alkanesulfonic Acids; C030641:alkylbenzyl sulfonic acid