title: [A novel retinoid CD437 induces apoptosis of human epidermoid carcinoma A431 cells].
abstract: OBJECTIVE
  To investigate the effect of a novel retinoid CD437 and all-trans retinoic acid (ATRA) in inducing cell apoptosis and inhibiting the proliferation of human epidermoid carcinoma A431 cells and normal human epidermal keratinocytes.
  
  
  METHODS
  MTT assay was used to determine the inhibitory effects of CD437 and ATRA on the growth of A431 cells and normal human epidermal keratinocytes, and the cell morphological changes were observed microscopically. Flow cytometry was used to investigate the effect of CD437 and ATRA on the cell cycle and apoptosis.
  
  
  RESULTS
  CD437 was more effective than ATRA in inhibiting the proliferation of A431 cells and normal human epidermal keratinocytes. CD437 increased the percentage of sub-G1 populations in A431 cells and induced G1 arrest in normal human epidermal keratinocytes. ATRA appeared to be relatively ineffective for inducing apoptosis in A431 cells as compared to CD437. CD437 did not duce obvious apoptosis in normal human epidermal keratinocytes.
  
  
  CONCLUSIONS
  CD437 is more effective than ATRA in inhibiting the proliferation and inducing apoptosis in A431 cells and shows selective apoptosis-inducing effect against malignant keratinocytes, suggesting its potential in the prevention or treatment of cutaneous carcinoma.
authors: Department of Dermatology, Second Hospital Affiliated to Xi'an Jiaotong University College of Medicine, Xi'an 710004, China. pan730412@126.com
authors: Min
authors: M
authors: Pan
authors: Zhen-hui
authors: ZH
authors: Peng
authors: Sheng-xiang
authors: SX
authors: Xiao
authors: Xiao-li
authors: XL
authors: Li
authors: Yan
authors: Y
authors: Liu
authors: Zheng-xiao
authors: ZX
authors: Li
authors: Jian-wen
authors: JW
authors: Ren
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004817:Epidermis / Q000187:drug effects / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D012176:Retinoids / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents; C099555:CD 437; D012176:Retinoids; D014212:Tretinoin