Development of capillary electrochromatography with poly(styrene-divinylbenzene-vinylbenzenesulfonic acid) monolith as the stationary phase.
A new polystyrene-based monolithic stationary phase, which was prepared by single step in situ copolymerization of styrene, divinylbenzene and vinylbenzenesulfonic acid (VBSA), was developed as a separation column for capillary electrochromatography, in which VBSA was employed as the charge-bearing monomer. Polymerization time of the polystyrene-based monolith had slightly influenced the separation time of the tested analytes, but it effectively altered their separation resolutions. Furthermore, baseline separation for a wider range of acetonitrile levels of mobile phase was achieved when a monolithic column prepared by a longer polymerization time was used. This novel polystyrene-based monolithic column provided an adequate electroosmotic flow either in basic or acidic mobile phase when VBSA level was maintained at 2.6% (w/w). Finally, this proposed polystyrene-based column allowed seven tested analytes to achieve a reproducible baseline separation within 2.2 min with theoretical plate numbers higher than 164 000 plates/m.
Huang
Hsi-Ya
HY
R&D Center for Membrane Technology, Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan Christian University, Chung-Li, Taiwan. hyhuang@cycu.edu.tw <hyhuang@cycu.edu.tw>
Liu
Yen-Chen
YC
Cheng
Yi-Jie
YJ
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
D011137:Polystyrenes; D011145:Polyvinyls; D013451:Sulfonic Acids; C530573:poly(styrene-divinylbenzene-vinylbenzenesulfonic acid)