Trypanosoma brucei mitochondrial ribosomes: affinity purification and component identification by mass spectrometry.
Although eukaryotic mitochondrial (mt) ribosomes evolved from a putative prokaryotic ancestor their compositions vary considerably among organisms. We determined the protein composition of tandem affinity-purified Trypanosoma brucei mt ribosomes by mass spectrometry and identified 133 proteins of which 77 were associated with the large subunit and 56 were associated with the small subunit. Comparisons with bacterial and mammalian mt ribosomal proteins identified T. brucei mt homologs of L2-4, L7/12, L9, L11, L13-17, L20-24, L27-30, L33, L38, L43, L46, L47, L49, L52, S5, S6, S8, S9, S11, S15-18, S29, and S34, although the degree of conservation varied widely. Sequence characteristics of some of the component proteins indicated apparent functions in rRNA modification and processing, protein assembly, and mitochondrial metabolism implying possible additional roles for these proteins. Nevertheless most of the identified proteins have no homology outside Kinetoplastida implying very low conservation and/or a divergent function in kinetoplastid mitochondria.
Zíková
Alena
A
Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
Panigrahi
Aswini K
AK
Dalley
Rachel A
RA
Acestor
Nathalie
N
Anupama
Atashi
A
Ogata
Yuko
Y
Myler
Peter J
PJ
Stuart
Kenneth
K
D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry; D054657:Ribosome Subunits / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D014346:Trypanosoma brucei brucei / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D012269:Ribosomal Proteins