Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR.
The transcriptional regulators RamA, RamB and GlxR were detected to bind to the promoter region of the resuscitation promoting factor 2 (rpf2) gene involved in growth and culturability of Corynebacterium glutamicum. DNA-binding sites were identified by bioinformatic analysis and verified by electrophoretic mobility shift assays with purified hexahistidyl-tagged proteins. Carbon source-dependent deregulation of rpf2 expression was demonstrated in vivo in ramA and ramB mutants and in a C. glutamicum strain overexpressing glxR. The deduced network of regulatory interactions provided insights into the complex regulation pattern of rpf2 expression in C. glutamicum.
Jungwirth
Britta
B
Centrum für Biotechnologie, Institut für Genomforschung und Systembiologie, Universität Bielefeld, Bielefeld, Germany.
Emer
Denise
D
Brune
Iris
I
Hansmeier
Nicole
N
Pühler
Alfred
A
Eikmanns
Bernhard J
BJ
Tauch
Andreas
A
D000085:Acetates / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001665:Binding Sites; D048230:Corynebacterium glutamicum / Q000235:genetics*; D000242:Cyclic AMP / Q000378:metabolism; D016207:Cytokines / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
D000085:Acetates; D001426:Bacterial Proteins; D016207:Cytokines; D014157:Transcription Factors; C113704:resuscitation-promoting factor, bacteria; D000242:Cyclic AMP