title: Chemically modified oligonucleotides with efficient RNase H response.
abstract: Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region. The RNA strand of duplexes with ON3, ON5 and ON12 were cleaved more efficiently than the RNA strand of the DNA:RNA control duplex. There seems to be no correlation between the thermal stability between the duplexes and RNase H cleavage.
authors: Nucleic Acid Center, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
authors: Birte
authors: B
authors: Vester
authors: Anne Marie
authors: AM
authors: Boel
authors: Sune
authors: S
authors: Lobedanz
authors: B Ravindra
authors: BR
authors: Babu
authors: Michael
authors: M
authors: Raunkjaer
authors: Dorthe
authors: D
authors: Lindegaard
authors: Raunak
authors: Patrick J
authors: PJ
authors: Hrdlicka
authors: Torben
authors: T
authors: Højland
authors: Pawan K
authors: PK
authors: Sharma
authors: Surender
authors: S
authors: Kumar
authors: Poul
authors: P
authors: Nielsen
authors: Jesper
authors: J
authors: Wengel
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D016914:Ribonuclease H / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D009841:Oligonucleotides; D012313:RNA; D004247:DNA; D016914:Ribonuclease H