title: Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay.
abstract: Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension, and ligation) assay in which expression of 502 genes are analyzed simultaneously using as little as 100 ng of input RNA.
authors: VM Institute of Research, Montreal, Quebec, Canada.
authors: Mark
authors: M
authors: Abramovitz
authors: Maja
authors: M
authors: Ordanic-Kodani
authors: Yuefang
authors: Y
authors: Wang
authors: Zhenhong
authors: Z
authors: Li
authors: Charles
authors: C
authors: Catzavelos
authors: Mark
authors: M
authors: Bouzyk
authors: George W
authors: GW
authors: Sledge
authors: Carlos S
authors: CS
authors: Moreno
authors: Brian
authors: B
authors: Leyland-Jones
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism*; D005557:Formaldehyde; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016612:Paraffin Embedding; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods
chemical_list: D009363:Neoplasm Proteins; D005557:Formaldehyde; D012313:RNA