Characterization of a novel gene encoding ankyrin repeat domain from Cotesia vestalis polydnavirus (CvBV).
Cotesia vestalis (Haliday) is an endoparasitoid of Plutella xylostella (L.) larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we describe the characterization of a gene (CvBV805) and its products. CvBV805 is located on the segment S8 of CvBV genome; it has a size of 909 bp and encodes a predicted protein of 125 amino acids. This protein contains an ankyrin repeat domain with a high degree of similarity with IkappaB-like genes. Gene transcripts were detected in extracts of the host as early as 2 h post-parasitization (p.p.) and continued to be detected through 24 h. Tissue-specific expression patterns showed that CvBV805 might be involved in early host immunosuppression. CvBV805 was detected in parasitized hosts at 12 h p.p. and in rBac-eGFP-CvBV805-infected Tn-5B1-4 cells at 72 h.p.i. by using western blots analysis. The size of the protein expressed in the host hemocytes and infected Tn-5B1-4 cells was 17 kDa and 56 kDa (including eGFP), respectively, which nearly corresponded with the predicted molecular weight (14.31 kDa) of CvBV805, suggesting that the protein did not undergo extensive post-translational modification. The protein was confirmed to be present within the nuclear region in hemocytes of the parasitized P. xylostella larvae at 48 h p.p. using confocal laser scanning microscopy.
Shi
Min
M
Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Chen
Ya-Feng
YF
Huang
Fang
F
Liu
Peng-Cheng
PC
Zhou
Xue-Ping
XP
Chen
Xue-Xin
XX
D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics*; D006434:Hemocytes / Q000821:virology; D007108:Immune Tolerance; D007814:Larva / Q000276:immunology / Q000821:virology; D018613:Microscopy, Confocal; D020527:Microscopy, Scanning Probe; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009036:Moths / Q000276:immunology / Q000821:virology*; D010802:Phylogeny; D018071:Polydnaviridae / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
D014764:Viral Proteins