[New approaches to the immunotherapy of Alzheimer's disease with the synthetic fragments of alpha7 subunit of the acetylcholine receptor].
The effect of immunization with the synthetic fragments of the alpha7 subunit of the acetylcholine nicotine receptor on the spatial memory of mice subjected to olfactory bulbectomy, which causes the development of the neuro-degenetrative disease of Alzheimer's type, was studied. Mice of the NMRI line were immunized with the KLH conjugates of two peptide fragments of the N-terminal fragment of the alpha7 subunit extraxcellular fragment, subjected to olfactory bulbectomy to cause the development of the neurodegenetrative disease of Alzheimer's type, and then the state of the spartial memory was evaluated. It was shown that 20% of bulbectomized mice immunized with the N-terminal 1-23 fragment exhibited good spatial memory after training. Immunization with the peptide construct (159-167)-(179-188) consisting of two hydrophilic exposed regions of alpha7-subunit induced good spatial memory in 50% of bulbectomized mice, while in the control group, which received only KLH, none of the animals were educated. Thus, the development of immunotherapy with peptide (159-167)-(179-188) seems to be a promising approach to prophylaxis and treatment of Alzheimer's disease.
Vol'pina
O M
OM
Volkova
T D
TD
Titova
M A
MA
Gershovich
Iu G
IuG
medvinskaia
N I
NI
Samokhin
A N
AN
Kamynina
A V
AV
Shalgunov
V S
VS
Koroev
D O
DO
Filatova
M P
MP
Oboznaia
M B
MB
Bobkova
N V
NV
D000544:Alzheimer Disease / Q000453:epidemiology / Q000276:immunology / Q000628:therapy*; D000818:Animals; D006433:Hemocyanins / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D007114:Immunization; D008568:Memory / Q000187:drug effects*; D051379:Mice; D021122:Protein Subunits / Q000276:immunology* / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000276:immunology*
D021122:Protein Subunits; D011978:Receptors, Nicotinic; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin