Biosensors for RNA aptamers-protein interaction.
Aptamers are an alternative to antibodies in their role as biorecognition elements in analytical devices. RNA aptamers, specific for different proteins, have been exploited as biorecognition elements to develop specific biosensors (aptasensors). These recognition elements have been coupled to piezoelectric quartz crystals and surface plasmon resonance (SPR) devices as transducers. The procedure for fixing the aptamer onto these transducers and for monitoring the interaction with the target protein is shown here.
Tombelli
Sara
S
Dipartimento di Chimica, Università degli Studi di Firenze, Sesto Fiorentino, Italy.
Minunni
Maria
M
Mascini
Marco
M
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D003460:Crystallization; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011791:Quartz; D016601:RNA-Binding Proteins / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000378:metabolism
D052157:Aptamers, Nucleotide; D016601:RNA-Binding Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D011791:Quartz