The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells.
To further understand the significance of bone as a target tissues of lead toxicity, as well as a reservoir of systemic lead, it is necessary to define the effect of lead on the calcium release activated calcium influx (CRACI) in primary cultures of human osteoblast-like cells (OLC). Pb2+ inhibited the immediate CRACI dose-dependent manner. Influx of Pb2+ into human OLC was increased dose-dependent manner. The present study demonstrates that the interference of Pb2+ with CRACI of human OLC is at least twofold: (1) the initiation of CRACI, i.e., the measurable influx of Ca2+ upon Ca2+ readdition, is partially inhibited by Pb2+ and (2) the influx of Pb2+ was enhanced after CRACI had been induced.
Jang
Hye-Ock
HO
Department of Dental Pharmacology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Korea.
Kim
Ji-Suk
JS
Kwon
Woo-Cheol
WC
Kim
Jeong-Kuk
JK
Ko
Myung-Suk
MS
Kim
Dong-Hoo
DH
Kim
Won-Il
WI
Jeon
Young-Chan
YC
Chung
In-Kyo
IK
Shin
Sang-Hun
SH
Chung
Jin
J
Bae
Moon-Kyung
MK
Yun
Il
I
D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007202:Indicators and Reagents; D007476:Ionophores / Q000494:pharmacology; D007854:Lead / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012996:Solutions; D019284:Thapsigargin / Q000494:pharmacology
D015220:Calcium Channels; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D007476:Ionophores; D012996:Solutions; D007854:Lead; D000001:Calcimycin; D019284:Thapsigargin; D002118:Calcium