Screening of volatile composition from Portuguese multifloral honeys using headspace solid-phase microextraction-gas chromatography-quadrupole mass spectrometry.
The volatile composition from four types of multifloral Portuguese (produced in Madeira Island) honeys was investigated by a suitable analytical procedure based on dynamic headspace solid-phase microextraction (HS-SPME) followed by thermal desorption gas chromatography-quadrupole mass spectrometry detection (GC-qMS). The performance of five commercially available SPME fibres: 100 microm polydimethylsiloxane, PDMS; 85 microm polyacrylate, PA; 50/30 microm divinylbenzene/carboxen on polydimethylsiloxane, DVB/CAR/PDMS (StableFlex); 75 microm carboxen/polydimethylsiloxane, CAR/PDMS, and 65 microm carbowax/divinylbenzene, CW/DVB; were evaluated and compared. The highest amounts of extract, in terms of the maximum signal obtained for the total volatile composition, were obtained with a DVB/CAR/PDMS coating fibre at 60 degrees C during an extraction time of 40 min with a constant stirring at 750 rpm, after saturating the sample with NaCl (30%). Using this methodology more than one hundred volatile compounds, belonging to different biosynthetic pathways were identified, including monoterpenols, C13-norisoprenoids, sesquiterpenes, higher alcohols, ethyl esters and fatty acids. The main components of the HS-SPME samples of honey were in average ethanol, hotrienol, benzeneacetaldehyde, furfural, trans-linalool oxide and 1,3-dihydroxy-2-propanone.
Pontes
M
M
Centro de Química da Madeira, Departamento de Química da Universidade da Madeira, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.
Marques
J C
JC
Câmara
J S
JS
D000180:Acrylic Resins / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006722:Honey / Q000032:analysis*; D011092:Polyethylene Glycols / Q000737:chemistry; D011174:Portugal; D012828:Silicones / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000295:instrumentation* / Q000379:methods; D014753:Vinyl Compounds / Q000737:chemistry; D014835:Volatilization
D000180:Acrylic Resins; D004129:Dimethylpolysiloxanes; D012828:Silicones; D014753:Vinyl Compounds; D011092:Polyethylene Glycols; C006903:carbopol 940; C013830:baysilon; C004985:divinyl benzene