Rapid aryl hydrocarbon receptor based polymerase chain reaction screening assay for polychlorinated dibenzo-p-dioxins and furans in soil and sediment.
A method for the estimation of polychlorinated dibenzo-p-dioxin and furan (PCCD/F) toxicity equivalent quotient (TEQ) of soil and sediment matrices is described. The method includes extraction, isolation of the PCDD/Fs from interfering compounds, such as polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs), and measurement of PCDD/F using the PROCEPT aryl hydrocarbon (AhR) receptor based polymerase chain reaction (PCR) assay. The values obtained using the PROCEPT assay correlate well with reference TEQ values generated from gas chromatography-high resolution mass spectrometry (GC-HRMS), with a linearity coefficient (R(2)) of 0.94. Applied in a screening mode at 50pg/g PCDD/F TEQ, the PROCEPT assay yielded five false positive results (2.6%) and no false negative results for 196 analyses of spiked soils and environmental samples obtained from US EPA Superfund sites.
McAlister
Daniel R
DR
Eichrom Technologies, LLC 8205 S Cass Avenue, Suite 106, Darien, IL 60561, United States. dmcalister@pgrf.com
Fern
Michael J
MJ
Allen
Randy L
RL
D001572:Benzofurans / Q000032:analysis*; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics*; D012989:Soil Pollutants / Q000032:analysis*
D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D012989:Soil Pollutants