title: Spread of colistin resistant non-mucoid Pseudomonas aeruginosa among chronically infected Danish cystic fibrosis patients.
abstract: BACKGROUND
  Colistin resistant Pseudomonas aeruginosa have rarely been reported in cystic fibrosis (CF) patients.
  
  
  METHODS
  We performed a 17-year prospective study on colistin susceptibility and compared our findings with clinical variables.
  
  
  RESULTS
  The first outbreak started in 1995 and lasted 5 years. It involved 27 CF patients who had inhaled colistin twice daily for a median of 10 years. Colistin resistant isolates persisted in individual patients for a median of 75 days after colistin was withdrawn. A second outbreak started in 2004. It involved 40 patients, 17 of whom were the same as in the first outbreak. Most resistant isolates belonged to two major clones that had similar genotypes in the two outbreaks. The P. aeruginosa isolates were all non-mucoid and they appeared in a group of chronically infected patients that had been admitted to the same ward for antibiotic treatment and had been followed at the same week-days in the outpatient clinic. Patients were individually isolated to avoid cross-infection and colistin inhalation was avoided in the CF outpatient clinic and in the ward after both outbreaks. Since 2004, no further spread has been observed.
  
  
  CONCLUSIONS
  It is important that the colistin resistant clones do not spread to non-infected patients since colistin is an important antibiotic for eradication of initial and intermittent P. aeruginosa colonisation.
authors: Department of Clinical Microbiology, Dept. 9301 and Danish Cystic fibrosis Centre, Dept. 5003, Rigshospitalet, Copenhagen, Denmark. hkj@cochrane.dk
authors: Helle Krogh
authors: HK
authors: Johansen
authors: Samuel M
authors: SM
authors: Moskowitz
authors: Oana
authors: O
authors: Ciofu
authors: Tacjana
authors: T
authors: Pressler
authors: Niels
authors: N
authors: Høiby
mesh_terms: D000280:Administration, Inhalation; D000293:Adolescent; D000328:Adult; D002648:Child; D003091:Colistin / Q000008:administration & dosage / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000382:microbiology* / Q000503:physiopathology; D003718:Denmark / Q000453:epidemiology; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D010356:Patient Isolation; D011446:Prospective Studies; D011552:Pseudomonas Infections / Q000453:epidemiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D012129:Respiratory Function Tests
chemical_list: D003091:Colistin