title: The effect of an anti-inflammatory pentapeptide produced by Entamoeba histolytica on gene expression in the U-937 monocytic cell line.
abstract: OBJECTIVE
  Evaluate the Monocyte Locomotion Inhibitory Factor (MLIF) effect upon the expression of genes encoding human cytokines, receptors and related factors in the human cell line U-937. MLIF (Met-Gln-Cys-Asn-Ser) is an anti-inflammatory pentapeptide produced by Entamoeba histolytica that inhibits many human monocyte functions.
  
  
  METHODS
  U-937 cell line cultured (24 hrs/RPMI). RNA extracted by Trizol method. 385 genes were analyzed on microarray membranes, complement by real-time RT-PCR and protein expression of some affected genes.
  
  
  RESULTS
  MLIF had a preferentially inhibitory effect on gene expression; four genes were over-expressed and 13 underexpressed in MILF vs. simple medium - constitutive expression. Three genes are over-expressed and 19 under-expressed in MLIF/PMA vs. PMA - induced expression.
  
  
  CONCLUSIONS
  Many modified genes are products regulated by the Nuclear Factor-kappaB and Mitogen Activated Protein Kinase pathways, suggesting MLIF involvement with these two major pathways for the modulation of the inflammation and immune responses.
authors: Centro Médico Nacional Siglo XXI, Unidad de Investigación Médica en Inmunología, Instituto Mexicano del Seguro Social, Hospital de Pediatría, Avenida Cuauhtémoc 330, Col. Doctores, C P 06720, México, D F, México.
authors: R
authors: R
authors: Silva-García
authors: I
authors: I
authors: Estrada-García
authors: R
authors: R
authors: Ramos-Payán
authors: A
authors: A
authors: Torres-Salazar
authors: M E
authors: ME
authors: Morales-Martínez
authors: D
authors: D
authors: Arenas-Aranda
authors: J A
authors: JA
authors: Giménez-Scherer
authors: F
authors: F
authors: Blanco-Favela
authors: M G
authors: MG
authors: Rico-Rosillo
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D008954:Models, Biological; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D009842:Oligopeptides; C430647:monocyte locomotion inhibitory factor; D020929:Mitogen-Activated Protein Kinase Kinases