FAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate.
Fatty acid translocase (FAT/CD36) is one of several proteins implicated in receptor-mediated uptake of long-chain fatty acids (LCFAs). We have tested whether levels of FAT/CD36 correlate with cellular oleic acid import, using a Tet-Off inducible transfected CHO cell line. Consistent with our previous findings, FAT/CD36 was enriched in lipid raft-derived detergent-resistant membranes (DRMs) that also contained caveolin-1, the marker protein of caveolae. Furthermore in transfected cells, plasma membrane FAT/CD36 co-localized extensively with the lipid raft-enriched ganglioside GM1, and partially with a caveolin-1-EGFP fusion protein. Nevertheless, even at high levels of expression, FAT/CD36 did not affect uptake of oleic acid. We propose that the ability of FAT/CD36 to mediate enhanced uptake of LCFAs is dependent on co-expression of other proteins or factors that are lacking in CHO cells.
Eyre
Nicholas S
NS
School of Molecular and Biomedical Science, University of Adelaide, Adelaide, SA, Australia.
Cleland
Leslie G
LG
Mayrhofer
Graham
G
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D018955:CD36 Antigens / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016466:CHO Cells; D021941:Caveolae / Q000737:chemistry / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004318:Doxycycline / Q000494:pharmacology; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism*; D014162:Transfection
D018955:CD36 Antigens; D019301:Oleic Acid; D004318:Doxycycline