title: Transient expression of homologous hairpin RNA interferes with PVY transmission by aphids.
abstract: Hairpin RNAs have been used to confer resistance to viruses in plants through RNA silencing. However, it has not been demonstrated that RNA silencing was effective against inoculation by aphids of non-persistently transmitted viruses, the major route of plant virus spread in nature. As a proof-of-principle strategy, we made use of Agrobacterium tumefaciens to transiently express a hairpin RNA homologous to Potato virus Y (PVY) in plant tissues. A complete and specific interference with aphid transmission of PVY was achieved by inducers of RNA silencing, as demonstrated by specific siRNAs accumulation in agroinfiltrated tissues. To our knowledge, this is the first report of successful interference with non-persistent transmission of a plant virus using RNA interference.
authors: Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, (CIB, CSIC) Campus de la Ciudad Universitaria, Av, Ramiro de Maeztu 9, 28040 Madrid, Spain. marisolvargasco@yahoo.com
authors: Marisol
authors: M
authors: Vargas
authors: Belén
authors: B
authors: Martínez-García
authors: José Ramón
authors: JR
authors: Díaz-Ruíz
authors: Francisco
authors: F
authors: Tenllado
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D000818:Animals; D001042:Aphids / Q000821:virology*; D020029:Base Pairing; D003001:Cloning, Molecular; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified / Q000382:microbiology / Q000821:virology; D017800:Potyvirus / Q000235:genetics* / Q000472:pathogenicity*; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D014026:Nicotiana / Q000382:microbiology / Q000821:virology
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral