Expression localization of Bmi1 in mice testis.
B cell-specific Moloney murine leukaemia virus integration site 1 (Bmi1), known as the first functional member of PcG (Polycomb Group) family, is supposed to be a key regulator of stem cell self-proliferation. In this study, we investigated its expression in testis and its impact on spermatogonia proliferation for better understanding of its role in spermatogenesis. Results showed that Bmi1 was expressed in undifferentiated spermatogonia (A(s), A(pr) and A(al) spermatogonia). Overexpression of BMI1 could promote spermatogonia proliferation, while repression of endogenous Bmi1 by RNAi resulted in inhibition of the proliferation.
Zhang
Shiqing
S
Institute of Basic Medical Sciences, Beijing 100850, People's Republic of China.
Li
Dexue
D
Li
Enzhong
E
Li
Lan
L
Wang
Junjun
J
Wang
Changyong
C
Lu
Jingyan
J
Zhang
Xueming
X
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D033481:Chromosomes, Mammalian / Q000235:genetics; D003001:Cloning, Molecular; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D034622:RNA Interference; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D018800:Thy-1 Antigens / Q000378:metabolism; D014162:Transfection
C489421:Bmi1 protein, mouse; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D018800:Thy-1 Antigens; D063150:Polycomb Repressive Complex 1; D019009:Proto-Oncogene Proteins c-kit