title: Conserved active site residues limit inhibition of a copper-containing nitrite reductase by small molecules.
abstract: The interaction of copper-containing dissimilatory nitrite reductase from Alcaligenes faecalis S-6 ( AfNiR) with each of five small molecules was studied using crystallography and steady-state kinetics. Structural studies revealed that each small molecule interacted with the oxidized catalytic type 2 copper of AfNiR. Three small molecules (formate, acetate and nitrate) mimic the substrate by having at least two oxygen atoms for bidentate coordination to the type 2 copper atom. These three anions bound to the copper ion in the same asymmetric, bidentate manner as nitrite. Consistent with their weak inhibition of the enzyme ( K i >50 mM), the Cu-O distances in these AfNiR-inhibitor complexes were approximately 0.15 A longer than that observed in the AfNiR-nitrite complex. The binding mode of each inhibitor is determined in part by steric interactions with the side chain of active site residue Ile257. Moreover, the side chain of Asp98, a conserved residue that hydrogen bonds to type 2 copper-bound nitrite and nitric oxide, was either disordered or pointed away from the inhibitors. Acetate and formate inhibited AfNiR in a mixed fashion, consistent with the occurrence of second acetate binding site in the AfNiR-acetate complex that occludes access to the type 2 copper. A fourth small molecule, nitrous oxide, bound to the oxidized metal in a side-on fashion reminiscent of nitric oxide to the reduced copper. Nevertheless, nitrous oxide bound at a farther distance from the metal. The fifth small molecule, azide, inhibited the reduction of nitrite by AfNiR most strongly ( K ic = 2.0 +/- 0.1 mM). This ligand bound to the type 2 copper center end-on with a Cu-N c distance of approximately 2 A, and was the only inhibitor to form a hydrogen bond with Asp98. Overall, the data substantiate the roles of Asp98 and Ile257 in discriminating substrate from other small anions.
authors: Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver BC, V6T 1Z3, Canada.
authors: Elitza I
authors: EI
authors: Tocheva
authors: Lindsay D
authors: LD
authors: Eltis
authors: Michael E P
authors: ME
authors: Murphy
mesh_terms: D000085:Acetates / Q000737:chemistry; D042481:Alcaligenes faecalis / Q000201:enzymology*; D001224:Aspartic Acid / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D004791:Enzyme Inhibitors / Q000737:chemistry*; D005561:Formates / Q000737:chemistry; D007532:Isoleucine / Q000737:chemistry*; D007700:Kinetics; D008958:Models, Molecular; D009566:Nitrates / Q000737:chemistry; D009572:Nitrite Reductases / Q000737:chemistry*
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D005561:Formates; D009566:Nitrates; D007532:Isoleucine; D001224:Aspartic Acid; D003300:Copper; D009572:Nitrite Reductases; C052695:nitrite reductase, copper-containing