Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections.
An ultra-sensitive method for glycan analysis targeting small tissue sections (1.5mm in diameter) is described as an application of a recently-established lectin microarray technology. The developed system achieved a high level of detection of a tissue section consisting of approximately 500 cells for differential profiling, where both N- and O-glycans attached to a pool of glycoproteins are subjected to multiplex analysis with 43 lectins. By using an optimized protocol for differential glycan analysis, sections of adenocarcinoma (n=28) and normal epithelia (n=12) of the colon were analyzed in an all-in-one manner. As a result, Wisteria floribunda agglutinin (WFA) was found to clearly differentiate cancerous from normal epithelia with P<0.0001. The obtained results correlated well with the subsequent histochemical study using biotinylated WFA. Thus, the developed technology proved to be valid for expanding the lectin microarray applications to tissue-based glycomics, and hence, should accelerate a discovery phase of glycan-related biomarkers.
Matsuda
Atsushi
A
Lectin Application and Analysis Team, Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 2, 1-1-1 Umezono, Tsukuba-shi, Ibaraki 305-8568, Japan.
Kuno
Atsushi
A
Ishida
Hiroyasu
H
Kawamoto
Toru
T
Shoda
Jun-Ichi
J
Hirabayashi
Jun
J
D000230:Adenocarcinoma / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000737:chemistry; D004210:Dissection; D005557:Formaldehyde / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D034781:Receptors, N-Acetylglucosamine / Q000737:chemistry; D046888:Tissue Array Analysis / Q000379:methods*; D016610:Tissue Embedding
D015415:Biomarkers; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D034781:Receptors, N-Acetylglucosamine; C040129:wisteria lectin; D005557:Formaldehyde