title: Determination of 8-iso-prostaglandin F(2alpha) in exhaled breath condensate using combination of immunoseparation and LC-ESI-MS/MS.
abstract: Rapid and precise method for the determination of 8-iso-prostaglandin F(2alpha), an essential marker of the oxidative stress, in exhaled breath condensate (EBC) was developed. The protocol consisted of stable isotope dilution, immunoseparation combined with selective and sensitive LC-ESI-MS/MS operated in multiple reaction monitoring (MRM) mode. The imprecision of the developed method was below 8.8%, the parameter of mean inaccuracy was determined as <9.6% (0-250pg of 8-iso-prostaglandin F(2alpha)/ml EBC). The limit of detection (LOD) was 1 pg/ml EBC and limit of quantification (LOQ) 5 pg/ml EBC. A significant difference in 8-iso-prostaglandin F(2alpha) content between the group of asbestosis patients and healthy volunteers was found.
authors: Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech Republic.
authors: Kamila
authors: K
authors: Syslová
authors: Petr
authors: P
authors: Kacer
authors: Marek
authors: M
authors: Kuzma
authors: Pavlína
authors: P
authors: Klusácková
authors: Zdena
authors: Z
authors: Fenclová
authors: Jindriska
authors: J
authors: Lebedová
authors: Daniela
authors: D
authors: Pelclová
mesh_terms: D001944:Breath Tests; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost