Differential expression analysis by cDNA-AFLP of Saccharum spp. after inoculation with the host pathogen Sporisorium scitamineum.
A differential expression study on the Saccharum spp.-Sporisorium scitamineum pathogenic interaction was carried out in the susceptible Ja60-5 and the resistant M31/45 genotypes. Using cDNA-AFLP analysis, a total of 64 transcript-derived fragments (TDFs) was found to be differentially expressed, with the majority (67.2%) of the differential TDFs up-regulated in the resistant M31/45 cultivar. The plant response against S. scitamineum infection was complex, representing major genes involved in oxidative burst, defensive response, ethylene and auxins pathways during the first 72 h post-inoculation. Results obtained suggest a key role for genes involved in the oxidative burst and the lignin pathways in the initial sugarcane defense against the S. scitamineum infection.
Lao
María
M
National Institute for Sugarcane Research, CUJAE Km 2 1/2 Road. Boyeros, 19390 Havana City, Cuba.
Arencibia
Ariel D
AD
Carmona
Elva R
ER
Acevedo
Ricardo
R
Rodríguez
Eida
E
León
Ondina
O
Santana
Ignacio
I
D054458:Amplified Fragment Length Polymorphism Analysis; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D005030:Ethylenes / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D007210:Indoleacetic Acids / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D010084:Oxidation-Reduction; D010935:Plant Diseases / Q000235:genetics; D031786:Saccharum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D014587:Ustilaginales / Q000502:physiology*
D018076:DNA, Complementary; D018744:DNA, Plant; D005030:Ethylenes; D007210:Indoleacetic Acids; D008031:Lignin; C036216:ethylene