title: Successful pregnancy and childbirth after intracytoplasmic sperm injection with calcium ionophore oocyte activation in a globozoospermic patient.
abstract: OBJECTIVE
  To check the effectiveness of intracytoplasmic sperm injection (ICSI) combined with assisted oocyte activation (AOA) in a globozoospermic patient.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Instituto Valenciano de Infertilidad, Valencia, Spain.
  
  
  METHODS
  A patient with globozoospermia.
  
  
  METHODS
  ICSI was administered in 14 oocytes. ICSI combined with AOA, in which a small amount of calcium was injected followed by calcium ionophore exposure, was done in 9 oocytes.
  
  
  METHODS
  Fertilization rate and embryo quality was assessed in both groups.
  
  
  RESULTS
  Chemical activation increased fertilization rate (55.6% vs. 35.7%) and the number of embryos with less multinucleation on day 2 (0 vs. 60%). Two embryos generated from AOA were transferred into the uterus (on day 3), resulting in a pregnancy and a healthy newborn.
  
  
  CONCLUSIONS
  The AOA with calcium ionophore treatment improved fertilization rate and quality of the embryos, and was found to be an effective method for AOA in this patient with a low fertilization rate after previous ICSI treatment.
authors: Instituto Valenciano de Infertilidad Bilbao, In Vitro Fertilization Laboratory, Vizcaya, Spain. atejera@ivi.es
authors: Alberto
authors: A
authors: Tejera
authors: Marta
authors: M
authors: Mollá
authors: Lourdes
authors: L
authors: Muriel
authors: Jose
authors: J
authors: Remohí
authors: Antonio
authors: A
authors: Pellicer
authors: Jose Luis
authors: JL
authors: De Pablo
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000175:diagnosis / Q000628:therapy*; D002118:Calcium / Q000008:administration & dosage*; D003131:Combined Modality Therapy; D005260:Female; D005303:Fertility Agents, Male / Q000008:administration & dosage*; D006801:Humans; D007476:Ionophores / Q000008:administration & dosage; D050498:Live Birth; D008297:Male; D009865:Oocytes / Q000187:drug effects*; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000187:drug effects* / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D005303:Fertility Agents, Male; D007476:Ionophores; D002118:Calcium