Therapeutic efficacy of Ac-DMQD-CHO, a caspase 3 inhibitor, for rat spinal cord injury.
We investigated the therapeutic efficacy of Ac-DMQD-CHO, a caspase-3 inhibitor, and functional recovery in spinal cord injury in a rat model. Thirty rats were randomized into three groups of 10 each. In groups 2 and 3, spinal cord trauma was produced in the thoracic region. Group 3 rats were treated with Ac-DMQD-CHO. Treatment responses were evaluated based on histopathological and TUNEL staining findings at 24 h and 5 days post-injury. Neurologic performance was assessed during and following treatment. Twenty-four hours after injury, light microscopy examination revealed diffuse hemorrhagic necrosis, edema, vascular thrombi, and polymorphonuclear leukocyte infiltration in group 2 and 3 rats, but cavitation and demyelinization were less prominent in group 3. At this time point, treatment of the rats with Ac-DMQD-CHO significantly reduced the number of apoptotic cells. Traumatic injury to the spinal cord causes apoptosis and administration of Ac-DMQD-CHO decreases apoptosis and improves functional outcome.
Akdemir
Osman
O
Department of Neurosurgery, Taksim Education and Research Hospital, Istanbul, Turkey.
Berksoy
Ismail
I
Karaoğlan
Alper
A
Barut
Seref
S
Bilguvar
Kaya
K
Cirakoğlu
Beyazit
B
Sahan
Elife
E
Colak
Ahmet
A
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002452:Cell Count / Q000379:methods; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D020287:In Situ Nick-End Labeling / Q000379:methods; D007796:Laminectomy / Q000379:methods; D009043:Motor Activity / Q000187:drug effects; D009842:Oligopeptides / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000150:complications / Q000188:drug therapy* / Q000209:etiology; D018709:Statistics, Nonparametric; D013997:Time Factors
D004791:Enzyme Inhibitors; D009842:Oligopeptides; C110922:acetyl-aspartyl-methionyl-glutaminyl-aspartyl-aldehyde