Electroanalytical investigation and determination of pefloxacin in pharmaceuticals and serum at boron-doped diamond and glassy carbon electrodes.
The anodic behavior and determination of pefloxacin on boron-doped diamond and glassy carbon electrodes were investigated using cyclic, linear sweep, differential pulse and square wave voltammetric techniques. In cyclic voltammetry, pefloxacin shows one main irreversible oxidation peak and additional one irreversible ill-defined wave depending on pH values for both electrodes. The results indicate that the process of pefloxacin is irreversible and diffusion controlled on boron-doped diamond electrode and irreversible but adsorption controlled on glassy carbon electrode. The peak current is found to be linear over the range of concentration 2x10(-6) to 2x10(-4)M in 0.5M H(2)SO(4) at about +1.20V (versus Ag/AgCl) for differential pulse and square wave voltammetric technique using boron-doped diamond electrode. The repeatability, reproducibility, precision and accuracy of the methods in all media were investigated. Selectivity, precision and accuracy of the developed methods were also checked by recovery studies. The procedures were successfully applied to the determination of the drug in pharmaceutical dosage forms and humans serum samples with good recovery results. No electroactive interferences from the excipients and endogenous substances were found in the pharmaceutical dosage forms and biological samples, respectively.
Uslu
Bengi
B
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Tandogan-Ankara, Turkey.
Topal
Burcu Dogan
BD
Ozkan
Sibel A
SA
D002244:Carbon; D018130:Diamond; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006801:Humans; D015366:Pefloxacin / Q000032:analysis* / Q000097:blood; D004364:Pharmaceutical Preparations / Q000737:chemistry; D015203:Reproducibility of Results
D004364:Pharmaceutical Preparations; D015366:Pefloxacin; D002244:Carbon; D018130:Diamond