Identification of novel metronidazole-inducible genes in Mycobacterium smegmatis using a customized amplification library.
The incidence of antibiotic resistance in pathogenic bacteria is rising. Bacterial resistance may be a natural defense of organisms, or it may result from spontaneous mutations or the acquisition of exogenous resistance genes. We grew spontaneous metronidazole-resistant Mycobacterium smegmatis mutants on solid medium cultures and employed differential expression using a customized amplification library to analyze the global gene profiles of metronidazole-resistant mutants under hypoxic conditions. In total, 66 genes involved in metronidazole resistance were identified and functionally characterized using the gene role category of M. smegmatis. Overall, genes associated with cell wall synthesis, such as methyltransferase and glycosyltransferase, and genes encoding drug transporters were highly expressed. The genes may be involved in the natural drug resistance of mycobacteria by increasing mycobacterial cell wall permeability and the efflux pumps of active drugs. In addition, the genes may play a role in dormancy. The genes identified in this study may lead to a better understanding of the mechanisms of metronidazole resistance during dormancy.
Kim
Su-Young
SY
Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, Korea.
Shin
Sung Jae
SJ
Song
Chang-Hwa
CH
Jo
Eun-Kyeong
EK
Kim
Hwa-Jung
HJ
Park
Jeong-Kyu
JK
D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015723:Gene Library; D005798:Genes, Bacterial / Q000187:drug effects* / Q000502:physiology; D008795:Metronidazole / Q000494:pharmacology*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D008795:Metronidazole