Expression of Foxo3a in non-Hodgkin's lymphomas is correlated with cell cycle inhibitor p27.
OBJECTIVE
Cell cycle arrest by FOXO transcription factors involves in transcriptional activation of p27(kip1), although the exact mechanism remains unclear. And it has been evidenced that reduced level of p27(kip1) which is frequently occurred in human cancers has been associated with poor prognosis. In this study, our purpose is to investigate the clinical relevance of altered patterns of Foxo3a and p27(kip1) expression in Chinese patients with localized non-Hodgkin's lymphomas (NHL).


METHODS
We analyzed the Foxo3a and p27(kip1) expression of Chinese NHL patients by immunohistochemistry and protein levels using Western Blot.


RESULTS
There was a direct relationship between the low level of Foxo3a and the rapid proliferation in immunohistochemical analyses. We also found a positive correlation between Foxo3a and p27(kip1) in immunohistochemical analyses and cell culture. Additionally we revealed that activation of Foxo3a could induce the accumulation of p27(kip1) at protein levels when cell cycle was arrested.


CONCLUSIONS
The expression of Foxo3a may be correlated with patients' survival.
Zhao
Yueming
Y
Department of Immunology, Medical College of Nantong University, Nantong, China.
Fei
Min
M
Wang
Yuchan
Y
Lu
Mudan
M
Cheng
Chun
C
Shen
Aiguo
A
D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins; D049109:Cell Proliferation; D002681:China; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000032:analysis* / Q000235:genetics; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011379:Prognosis
D018797:Cell Cycle Proteins; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D050760:Cyclin-Dependent Kinase Inhibitor p27