title: Optimum diagnostic assay and clinical specimen for routine rotavirus surveillance.
abstract: Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.
authors: National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Lstockman@cdc.gov
authors: Lauren J
authors: LJ
authors: Stockman
authors: Mary A
authors: MA
authors: Staat
authors: Michol
authors: M
authors: Holloway
authors: David I
authors: DI
authors: Bernstein
authors: Tara
authors: T
authors: Kerin
authors: Jennifer
authors: J
authors: Hull
authors: Eileen
authors: E
authors: Yee
authors: Jon
authors: J
authors: Gentsch
authors: Umesh D
authors: UD
authors: Parashar
mesh_terms: D002675:Child, Preschool; D005243:Feces / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D008403:Mass Screening / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*