Radiosynthesis and in vivo evaluation of N-[11C]methylated imidazopyridineacetamides as PET tracers for peripheral benzodiazepine receptors.
Imidazopyridineacetoamide 5-8, a series of novel and potentially selective peripheral benzodiazepine receptor (PBR) ligands with affinities comparable to those of known PBR ligands, was investigated. Radiosyntheses of [11C]5, 6, 7 or 8 was accomplished by N-methylation of the corresponding desmethyl precursors with [11C]methyl iodide in the presence of NaH in dimethylformamide (DMF), resulting in 25% to 77% radiochemical yield and specific activitiy of 20 to 150 MBq/nmol. Each of the labeled compounds was injected in ddY mice, and the radioactivity and weight of dissected peripheral organs and brain regions were measured. Organ distribution of [11C]7 was consistent with the known PBR distribution. Moreover, [11C]7 showed the best combination of brain uptake and PBR binding, leading to its high retention in the olfactory bulb and cerebellum, areas where PBR density is high in mouse brain. Coinjection of PK11195 or unlabeled 7 significantly reduced the brain uptake of [11C]7. These results suggest that [11C]7 could be a useful radioligand for positron emission tomography imaging of PBRs.
Sekimata
Katsuhiko
K
Department of Brain Sciences and Molecular Imaging, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522, Japan.
Hatano
Kentaro
K
Ogawa
Mikako
M
Abe
Junichiro
J
Magata
Yasuhiro
Y
Biggio
Giovanni
G
Serra
Mariangela
M
Laquintana
Valentino
V
Denora
Nunzio
N
Latrofa
Andrea
A
Trapani
Giuseppe
G
Liso
Gaetano
G
Ito
Kengo
K
D000081:Acetamides / Q000737:chemistry; D000818:Animals; D001667:Binding, Competitive; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging; D001921:Brain / Q000000981:diagnostic imaging; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D006321:Heart / Q000000981:diagnostic imaging; D007093:Imidazoles / Q000737:chemistry; D007546:Isoquinolines / Q000493:pharmacokinetics; D007668:Kidney / Q000000981:diagnostic imaging; D008099:Liver / Q000000981:diagnostic imaging; D008168:Lung / Q000000981:diagnostic imaging; D008297:Male; D008657:Metabolic Clearance Rate; D008745:Methylation; D051379:Mice; D008815:Mice, Inbred Strains; D049268:Positron-Emission Tomography / Q000379:methods; D011725:Pyridines / Q000737:chemistry; D011869:Radioligand Assay; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000493:pharmacokinetics*; D011963:Receptors, GABA-A / Q000378:metabolism*; D013154:Spleen / Q000000981:diagnostic imaging; D014018:Tissue Distribution
D000081:Acetamides; D002250:Carbon Radioisotopes; D007093:Imidazoles; D007546:Isoquinolines; D011725:Pyridines; D019275:Radiopharmaceuticals; D011963:Receptors, GABA-A; C029899:imidazole; C037850:PK 11195