Non-invasive imaging of epileptic seizures in vivo using photoacoustic tomography.
Non-invasive laser-induced photoacoustic tomography (PAT) is an emerging imaging modality that has the potential to image the dynamic function of the brain due to its unique ability of imaging biological tissues with high optical contrast and ultrasound resolution. Here we report the first application of our finite-element-based PAT for imaging of epileptic seizures in an animal model. In vivo photoacoustic images were obtained in rats with focal seizures induced by microinjection of bicuculline, a GABA(A) antagonist, into the neocortex. The seizure focus was accurately localized by PAT as confirmed with gold-standard electroencephalogram (EEG). Compared to the existing neuroimaging modalities, PAT not only has the unprecedented advantage of high spatial and temporal resolution in a single imaging modality, but also is portable and low in cost, making it possible to bring brain imaging to the bedside.
Zhang
Qizhi
Q
The J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USA.
Liu
Zhao
Z
Carney
Paul R
PR
Yuan
Zhen
Z
Chen
Huanxin
H
Roper
Steven N
SN
Jiang
Huabei
H
D000162:Acoustics; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000473:pathology*; D003292:Convulsants / Q000494:pharmacology; D004195:Disease Models, Animal; D004569:Electroencephalography / Q000379:methods; D004827:Epilepsy / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods*; D014463:Ultrasonography / Q000379:methods
D003292:Convulsants; D001640:Bicuculline