title: Tissue damage in rat ovaries subjected to torsion and detorsion: effects of L-carnitine and N-acetyl cysteine.
abstract: We aimed to evaluate histopathological changes, to detect HIF-1alpha staining intensities and to determine MDA levels in rat ovaries, which were subjected to torsion and detorsion and treated with L -carnitine or N-acetyl cysteine (NAC). Forty-eight prepubertal female Sprague-Dawley rats were divided into five groups (n = 8): 1, control; 2, ischemia; 3, reperfusion; 4, L -carnitine; and 5, NAC groups. In groups 3, 4 and 5, an ischemic period of 3 h was followed by reperfusion for 24 h. In groups 4 and 5, ischemia was performed and either L -carnitine or NAC was infused intraperitoneally 30 min before reperfusion. Ovarian tissues were examined histopathologically; tissue MDA levels and serum IL-6 levels were determined biochemically. HIF-1alpha was applied to all ovaries immunohistochemically. Total tissue damage scores, tissue MDA levels and HIF-1alpha scores, were significantly higher in group 2 (all P < 0.001) than group 4, and group 3 than group 4 (P < 0.001, P = 0.05 and P < 0.001, respectively). They were also significantly higher in group 2 (all P < 0.001) than group 5. When group 3 is compared to group 5, total tissue damage scores and tissue MDA levels were significantly higher in the former (P < 0.01 and P < 0.001, respectively). Serum IL-6 levels were significantly higher in group 2 when compared to groups 1, 4 and 5 (all P < 0.01). The degree of tissue damage of the torsioned ovaries decreased after a reperfusion period of 24 h in the torsioned ovaries. However, ovaries of both L -carnitine and NAC groups showed better recovery than the reperfusion group.
authors: Department of Pathology, Trakya University, Edirne, Turkey. drufukusta@yahoo.com
authors: Ufuk
authors: U
authors: Usta
authors: Mustafa
authors: M
authors: Inan
authors: Hakan
authors: H
authors: Erbas
authors: Nurettin
authors: N
authors: Aydogdu
authors: Fulya
authors: F
authors: Oz Puyan
authors: Semsi
authors: S
authors: Altaner
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D002331:Carnitine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016166:Free Radical Scavengers / Q000627:therapeutic use; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000097:blood; D008315:Malondialdehyde / Q000378:metabolism; D010049:Ovarian Diseases / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D010053:Ovary / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy / Q000209:etiology / Q000473:pathology*; D012720:Severity of Illness Index; D014102:Torsion Abnormality / Q000150:complications / Q000188:drug therapy / Q000473:pathology*; D054159:Torsion, Mechanical; D014803:Vitamin B Complex / Q000627:therapeutic use
chemical_list: D016166:Free Radical Scavengers; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D015850:Interleukin-6; D014803:Vitamin B Complex; D008315:Malondialdehyde; D002331:Carnitine; D000111:Acetylcysteine