title: Chimeras of bacterial translation factors Tet(O) and EF-G.
abstract: Ribosomal protection proteins (RPPs) confer bacterial resistance to tetracycline by releasing this antibiotic from ribosomes stalled in protein synthesis. RPPs share structural similarity to elongation factor G (EF-G), which promotes ribosomal translocation during normal protein synthesis. We constructed and functionally characterized chimeric proteins of Campylobacter jejuni Tet(O), the best characterized RPP, and Escherichia coli EF-G. A distinctly conserved loop sequence at the tip of domain 4 is required for both factor-specific functions. Domains 3-5: (i) are necessary, but not sufficient, for functional specificity; and (ii) modulate GTP hydrolysis by EF-G, while minimally affecting Tet(O), under substrate turnover conditions.
authors: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
authors: Nehal S
authors: NS
authors: Thakor
authors: Roxana
authors: R
authors: Nechifor
authors: Paul G
authors: PG
authors: Scott
authors: Monika
authors: M
authors: Keelan
authors: Diane E
authors: DE
authors: Taylor
authors: Kevin S
authors: KS
authors: Wilson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008958:Models, Molecular; D020653:Peptide Elongation Factor G / Q000737:chemistry / Q000235:genetics*; D014176:Protein Biosynthesis; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D020653:Peptide Elongation Factor G; D011993:Recombinant Fusion Proteins; C083139:Tet O resistance protein, Bacteria