title: In situ detection of plant viruses and virus-specific products.
abstract: The ability to combine nucleic acid hybridisation or immunospecific reactions with structural and ultrastructural analysis of virus-infected tissues has provided the opportunity to resolve the spatial details of infection with respect to the production of virus-specific products and the nature of the host response. These technologies may seem lengthy and complex but offer high rewards in terms of revealing the details of host-virus interactions not otherwise accessible.
authors: John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
authors: Andrew J
authors: AJ
authors: Maule
authors: Zoltán
authors: Z
authors: Havelda
mesh_terms: D018548:Cotyledon / Q000648:ultrastructure / Q000821:virology; D003890:Desiccation / Q000379:methods; D006790:Host-Parasite Interactions; D018519:Meristem / Q000821:virology; D008854:Microscopy, Electron / Q000379:methods; D009693:Nucleic Acid Hybridization; D018532:Pisum sativum / Q000821:virology; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D010944:Plants / Q000821:virology*; D016707:Tissue Fixation / Q000379:methods