Microarray expression profiling of human dental pulp from single subject.
BACKGROUND
Microarray is a recently developed simultaneous analysis of expression patterns of thousand of genes. The aim of this research was to evaluate the expression profile of human healthy dental pulp in order to find the presence of genes activated and encoding for proteins involved in the physiological process of human dental pulp. We report data obtained by analyzing expression profiles of human tooth pulp from single subjects, using an approach based on the amplification of the total RNA.


METHODS
Experiments were performed on a high-density array able to analyse about 21,000 oligonucleotide sequences of about 70 bases in duplicate, using an approach based on the amplification of the total RNA from the pulp of a single tooth. Obtained data were analyzed using the S.A.M. system (Significance Analysis of Microarray) and genes were merged according to their molecular functions and biological process by the Onto-Express software.


RESULTS
The microarray analysis revealed 362 genes with specific pulp expression. Genes showing significant high expression were classified in genes involved in tooth development, protoncogenes, genes of collagen, DNAse, Metallopeptidases and Growth factors.


CONCLUSIONS
We report a microarray analysis, carried out by extraction of total RNA from specimens of healthy human dental pulp tissue. This approach represents a powerful tool in the study of human normal and pathological pulp, allowing minimization of the genetic variability due to the pooling of samples from different individuals.
Tete
Stefano
S
Department of Oral Science, University G. d' Annunzio, Chieti, Italy.
Mastrangelo
Filiberto
F
Scioletti
Anna Paola
AP
Tranasi
Michelangelo
M
Raicu
Florina
F
Paolantonio
Michele
M
Stuppia
Liborio
L
Vinci
Raffaele
R
Gherlone
Enrico
E
Ciampoli
Cristian
C
Sberna
Maria Teresa
MT
Conti
Pio
P
D000293:Adolescent; D000483:Alleles; D030541:Databases, Genetic; D003782:Dental Pulp / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D014644:Genetic Variation; D006801:Humans; D046228:Microarray Analysis; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012313:RNA / Q000378:metabolism; D012984:Software
D012313:RNA