Detection and species identification of Cryptosporidium from Taiwan feeding animals.
In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Cryptosporidium spp. oocysts. Ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of Cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. Nucleic acid sequencing of the 18S rRNA gene identified 3 clusters of Cryptosporidium spp. Three Cryptosporidium parvum isolates were from cattle and sheep feces. One Cryptosporidium andersoni isolate was detected from pig feces. The other 2 novel Cryptosporidium genotypes were not similar to any known Cryptosporidium spp. according to the DNA sequences of the 18S rRNA gene.
Hsu
Bing-Mu
BM
Department of Earth and Environmental Sciences, National Chung Cheng University, 168, University Road, Min-Hsiung, Chiayi, Taiwan, ROC. bmhsu@eq.ccu.edu.tw
Wun
Hau-Yang
HY
Hsu
Chih-Li Lilian
CL
D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology; D002645:Chickens; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology; D006736:Horses; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology; D015800:Protozoan Proteins / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology; D013624:Taiwan / Q000453:epidemiology
D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S