Enhancing ultrasound-mediated cell membrane permeabilisation (sonoporation) using a high frequency pulse regime and implications for ultrasound-aided cancer chemotherapy.
Delivering ultrasound to HeLa cells at 1MHz using a high frequency pulse regime (40kHz) and at a maximum energy density of 270Jcm(-2) resulted in significant cell membrane permeabilisation. Using FITC-dextran as a fluorogenic marker, optimally up to 64% of treated populations were permeabilised with cell viability remaining above 80%. Although cell membrane permeabilisation was observed in the presence of the microbubble-based ultrasound contrast agent, SonoVue, cell viability was severely compromised. Using the high frequency pulse regime in the absence of microbubbles, the LD50 of the cancer chemotherapeutic agent, camptothecin, was reduced from 58 to 18nM.
Li
Ying Suet
YS
School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine, Co. Derry BT52 1SA, Northern Ireland, United Kingdom.
Reid
Cherith N
CN
McHale
Anthony P
AP
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000378:metabolism / Q000633:toxicity; D002166:Camptothecin / Q000008:administration & dosage* / Q000378:metabolism / Q000633:toxicity; D002463:Cell Membrane Permeability; D003131:Combined Modality Therapy; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy* / Q000628:therapy; D014465:Ultrasonics
D000972:Antineoplastic Agents, Phytogenic; D003911:Dextrans; D005456:Fluorescent Dyes; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate; D002166:Camptothecin