title: Differential expression of proteins from cultured endothelial cells exposed to uremic versus normal serum.
abstract: BACKGROUND
  Deficient hemostasis and accelerated atherosclerosis coexist in patients with chronic kidney disease. Endothelial dysfunction may be involved in the high incidence of atherothrombotic events in these patients. We established an in vitro model of endothelial dysfunction by exposing endothelial cells to uremic media and applied a proteomic approach to characterize endothelial cell dysfunction in uremia.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  Serum samples from 8 patients with chronic kidney disease on hemodialysis treatment were collected.
  
  
  METHODS
  Exposure of cultured endothelial cells to normal and uremic serum. OUTCOME AND MEASUREMENTS: Proteins from lysed cells were characterized by isoelectric point and molecular weight by using 2-dimensional gel electrophoresis. Spots were visualized by means of silver staining and identified by using mass spectrometry.
  
  
  RESULTS
  Identification of the most prominent proteins showed molecules related to inflammation (high mobility group box 1, aldose reductase, and proteasome components) and oxidative stress (superoxide dismutase and glutathione peroxidase), both associated with chronic kidney disease. These changes may be caused by activation of the nuclear factor-kappaB transcription factor. Changes in expression of cytoskeletal proteins (destrin and vimentin) also were detected.
  
  
  CONCLUSIONS
  In vitro study.
  
  
  CONCLUSIONS
  Proteomic techniques proved to be a powerful tool to investigate endothelial dysfunction in uremia. A more exhaustive analysis will provide answers and potential therapeutic targets in the near future.
authors: Servicio de Hemoterapia-Hemostasia y, Centre de Diagnòstic Biomèdic, Institut d'Investigacions biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
authors: Carla
authors: C
authors: Carbó
authors: Gemma
authors: G
authors: Arderiu
authors: Ginés
authors: G
authors: Escolar
authors: Berta
authors: B
authors: Fusté
authors: Aleix
authors: A
authors: Cases
authors: Montserrat
authors: M
authors: Carrascal
authors: Joaquín
authors: J
authors: Abián
authors: Maribel
authors: M
authors: Díaz-Ricart
mesh_terms: D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D002908:Chronic Disease; D003430:Cross-Sectional Studies; D042783:Endothelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D007674:Kidney Diseases / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014176:Protein Biosynthesis; D014511:Uremia / Q000378:metabolism