Radiolabeling for two-dimensional gel analysis.
Radiolabeling is a highly sensitive method for protein detection, which is easily performed by the incorporation of radioactive amino acids into proteins. This makes radiolabeling a method of choice for visualizing proteins separated on two-dimensional (2-D) gels. This chapter presents protocols to determine in vivo labeling conditions and to label proteins for the comparison of protein samples by means of 2-D gel electrophoresis.
Boucherie
Hélian
H
Centre National de la Recherche Scientifique, Bordeaux, France.
Massoni
Aurélie
A
Monribot-Espagne
Christelle
C
D000596:Amino Acids / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007553:Isotope Labeling / Q000379:methods*
D000596:Amino Acids