title: Clonal transmission of ESBL-producing Klebsiella spp. at a university hospital in Brazil.
abstract: The present study was designed to determine the prevalence and extended-spectrum beta-lactamase (ESBL) types in clinical isolates of Klebsiella spp. at a university hospital located in the Brazilian southern region (Ribeirão Preto, São Paulo) as well as their antibiotic susceptibility and genetic profiles. This study included 147 non-repeat Klebsiella spp. isolates collected from January to June 2000, of which 23 K. pneumoniae and 8 K. oxytoca were selected as ESBL producers by using the Oxoid combination disk method and Etest ESBL strip. beta-lactamases were characterized by IEF, PCR and sequencing assays using primers for ESBL genes. Antibiotic susceptibility was evaluated by MicroScan system. Dissemination of two major clones of ESBL-producing Klebsiella spp. occurred in the hospital. According to the results obtained in this study there was a clonal spread of CTX-M-producing K. oxytoca in five clinics and dissemination of ESBL-producing K. pneumoniae in the nursery and pediatrics wards.
authors: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil.
authors: Luciene A R
authors: LA
authors: Minarini
authors: Eduardo C
authors: EC
authors: Clímaco
authors: Daniela B
authors: DB
authors: Guimarães
authors: Joseane C
authors: JC
authors: Ferreira
authors: Izabel C V
authors: IC
authors: Palazzo
authors: Roberto
authors: R
authors: Martinez
authors: Ana Lúcia C
authors: AL
authors: Darini
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D001938:Brazil / Q000453:epidemiology; D024901:Drug Resistance, Multiple, Bacterial; D006785:Hospitals, University; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D008826:Microbial Sensitivity Tests; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001618:beta-Lactamases