Quantum dots-bienzyme hybrid system for the sensitive determination of glucose.
In this paper, we attempt to develop a sensitive detection method for glucose with the combination of the unique optical property of quantum dots and the specificity of enzymatic reactions. With glucose and hydroquinone as substrates, benzoquinone that intensively quenches the photoluminescence of quantum dots can be produced via the catalysis of bienzyme (glucose oxidase and horseradish peroxidase) system. A relatively low detection limit of 1.0x10(-8)mol/L can be achieved. Two linear ranges from 1.0x10(-6) to 1.5x10(-4)M and from 1.5x10(-4) to 1.0x10(-3)M were obtained. For the detection of 1.0x10(-4)M glucose, six replicative measurements showed the reproducibility (R.S.D.) of 4.43%. The quantum dots-enzymes system possesses advantages of simple procedure without modification of quantum dots or immobilization of enzymes, low cost, high sensitivity and short detection times within several minutes.
Yuan
Jipei
J
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Guo
Weiwei
W
Wang
Erkang
E
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis* / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018511:Systems Integration
D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose