Antithrombogenic polynitrosated polyester/poly(methyl methacrylate) blend for the coating of blood-contacting surfaces.
A nitric oxide (NO) donor polyester containing multiple S-nitrosothiol (S-NO) groups covalently attached to the polymer backbone was synthesized through the esterification of poly(ethylene glycol) with mercaptosuccinic acid, followed by the nitrosation of the -SH moieties. The polynitrosated polyester (PNPE) obtained was blended with poly(methyl methacrylate) (PMMA), yielding solid films capable of releasing NO. Scanning electron microscopy analysis showed that acrylic plates and stainless steel intracoronary stents can be coated with continuous and adherent PNPE/PMMA films. After an initial NO burst, these films release NO spontaneously in dry condition or immersed in aqueous solution at constant rates of 1.8 and 180 nmol/g/h, respectively, for more than 24 h at physiological temperature. PNPE/PMMA coated surfaces were shown to inhibit platelet adhesion when in contact with whole blood. These results show that PNPE/PMMA blend can be used for the coating of blood-contacting surfaces, with potential to inhibit thrombosis and restenosis after stenting.
Seabra
Amedea B
AB
Chemistry Institute, State University of Campinas, UNICAMP, Campinas, São Paulo, Brazil.
da Silva
Regiane
R
de Souza
Gabriela F P
GF
de Oliveira
Marcelo G
MG
D000179:Acrylates / Q000737:chemistry; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D023903:Coronary Restenosis / Q000378:metabolism / Q000517:prevention & control; D003692:Delayed-Action Preparations; D054855:Drug-Eluting Stents; D005343:Fibrinolytic Agents / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008422:Materials Testing; D009569:Nitric Oxide / Q000378:metabolism; D020030:Nitric Oxide Donors / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011091:Polyesters / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D011474:Prosthesis Design; D026403:S-Nitrosothiols / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties; D013927:Thrombosis / Q000378:metabolism / Q000517:prevention & control*
D000179:Acrylates; D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D005343:Fibrinolytic Agents; D020030:Nitric Oxide Donors; D011091:Polyesters; D026403:S-Nitrosothiols; D013193:Stainless Steel; D009569:Nitric Oxide; D019904:Polymethyl Methacrylate