Rhodamine-based fluorogenic probe for imaging biological thiol.
We have developed a new fluorescent probe for biological thiol. The probe was synthesized by the modification of the 2,4-dinitrobenzenesulfonyl group with rhodamine 110. The selective detection of thiol species such as cysteine or glutathione was achieved in biological conditions. Moreover, the probe was successfully applied to the imaging of thiol species in living human cells.
Shibata
Aya
A
Nano Medical Engineering Laboratory Discovery Research Institute, RIKEN 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Furukawa
Kazuhiro
K
Abe
Hiroshi
H
Tsuneda
Satoshi
S
Ito
Yoshihiro
Y
D001557:Benzenesulfonates / Q000737:chemistry; D002460:Cell Line; D003545:Cysteine / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D019793:Fluorescein / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D005978:Glutathione / Q000737:chemistry; D006801:Humans; D008956:Models, Chemical; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D012235:Rhodamines; D013438:Sulfhydryl Compounds; C049025:rhodamine 110; D005978:Glutathione; D003545:Cysteine; D019793:Fluorescein