Phenol biosensor based on Sonogel-Carbon transducer with tyrosinase alumina sol-gel immobilization.
A new biosensor for detection of phenols, based on tyrosinase immobilization with alumina sol-gel on Sonogel-Carbon transducer, has been developed. The electrode was prepared using high energy ultrasounds directly applied to the precursors. The alumina sol-gel provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenols are oxidized by tyrosinase biosensor to form a detectable product, which was determined at -300 mV vs. Ag/AgCl reference electrode. For phenol, the sensor exhibited a fast response which resulted from the porous structure and high enzyme loading of the sol-gel matrix. The linear range was from 5 x 10(-7)M to 3 x 10(-5)M, with a detection limit of 3 x 10(-7)M. The stability of the biosensor was also evaluated.
Zejli
H
H
IMAGES EA 4218, Centre de phytopharmacie, Université de PerpignanVia Domitia, Perpignan Cedex, France.
Hidalgo-Hidalgo de Cisneros
J L
JL
Naranjo-Rodriguez
I
I
Liu
Baohong
B
Temsamani
K R
KR
Marty
J L
JL
D000363:Agaricales / Q000201:enzymology; D000537:Aluminum Oxide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D014159:Transducers
D004800:Enzymes, Immobilized; D005782:Gels; D010636:Phenols; D012996:Solutions; D002244:Carbon; D014442:Monophenol Monooxygenase; D000537:Aluminum Oxide