title: Evolution of the arginase fold and functional diversity.
abstract: Novel structural superfamilies can be identified among the large number of protein structures deposited in the Protein Data Bank based on conservation of fold in addition to conservation of amino acid sequence. Since sequence diverges more rapidly than fold in protein Evolution, proteins with little or no significant sequence identity are occasionally observed to adopt similar folds, thereby reflecting unanticipated evolutionary relationships. Here, we review the unique alpha/beta fold first observed in the manganese metalloenzyme rat liver arginase, consisting of a parallel eight-stranded beta-sheet surrounded by several helices, and its evolutionary relationship with the zinc-requiring and/or iron-requiring histone deacetylases and acetylpolyamine amidohydrolases. Structural comparisons reveal key features of the core alpha/beta fold that contribute to the divergent metal ion specificity and stoichiometry required for the chemical and biological functions of these enzymes.
authors: Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA.
authors: D P
authors: DP
authors: Dowling
authors: L
authors: L
authors: Di Costanzo
authors: H A
authors: HA
authors: Gennadios
authors: D W
authors: DW
authors: Christianson
mesh_terms: D000595:Amino Acid Sequence; D000619:Aminohydrolases / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D000818:Animals; D001119:Arginase / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D001665:Binding Sites; D019143:Evolution, Molecular; D006655:Histone Deacetylases / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D006801:Humans; D008099:Liver / Q000201:enzymology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D051381:Rats; D017386:Sequence Homology, Amino Acid; D000581:Amidohydrolases
chemical_list: D000619:Aminohydrolases; D001119:Arginase; D006655:Histone Deacetylases; C058087:acetylpolyamine amidohydrolase; D000581:Amidohydrolases