title: The characteristics of action potential and nonselective cation current of cardiomyocytes in rabbit superior vena cava.
abstract: As a special focus in initiating and maintaining atrial fibrillation (AF), cardiomyocytes in superior vena cava (SVC) have distinctive electrophysiological characters. In this study, we found that comparing with the right atrial (RA) cardiomyocytes, the SVC cardiomyocytes had longer APD90 at the different basic cycle lengths; the conduction block could be observed on both RA and SVC cardiomyocytes. A few of SVC cardiomyocytes showed slow response action potentials with automatic activity and some others showed early after depolarization (EAD) spontaneously. Further more, we found that there are nonselective cation current (INs) in both SVC and RA cardiomyocytes. The peak density of I Ns in SVC cardiomyocytes was smaller than that in RA cardiomyocytes. Removal of extracellular divalent cation and glucose could increase INs in SVC cardiomyocytes. The agonist or the antagonist of INs may increase or decrease APD. To sum up, some SVC cardiomyocytes possess the ability of spontaneous activity; the difference of transmembrane action potentials between SVC and RA cardiomyocytes is partly because of the different density of INs between them; the agonist or the antagonist of INs can increase or decrease APD leading to the enhancement or reduction of EAD genesis in SVC cardiomyocytes. INs in rabbit myocytes is fairly similar to TRPC3 current in electrophysiological property, which might play an important role in the mechanisms of AF.
authors: Beijing ChaoYang Hospital, Capital Medical University, Beijing 100020, China. diudiupan@hotmail.com
authors: Pan
authors: P
authors: Wang
authors: XinChun
authors: X
authors: Yang
authors: XiuLan
authors: X
authors: Liu
authors: RongFeng
authors: R
authors: Bao
authors: TaiFeng
authors: T
authors: Liu
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000378:metabolism; D015761:4-Aminopyridine / Q000378:metabolism; D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D001145:Arrhythmias, Cardiac / Q000378:metabolism; D001281:Atrial Fibrillation / Q000378:metabolism; D002412:Cations / Q000378:metabolism*; D002586:Cesium / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers / Q000378:metabolism; D011817:Rabbits; D014683:Vena Cava, Superior / Q000166:cytology*
chemical_list: D002412:Cations; D007473:Ion Channels; D026902:Potassium Channel Blockers; D002586:Cesium; D015761:4-Aminopyridine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid