title: Repetitive postprandial hypertriglyceridemia induces monocyte adhesion to aortic endothelial cells in Goto-Kakizaki rats.
abstract: To compare the effects of postprandial hypertriglyceridemia and postprandial hyperglycemia on monocyte adhesion to endothelial cells, we investigated the effects of twice-daily standard diet (5% fat) and high-fat diet (30% fat) for 3 weeks on monocyte adhesion to endothelial cells and the expression of adhesion molecules in the aortic artery in non-obese type 2 diabetic Goto-Kakizaki rats. Fasting glucose, insulin, non-esterified fatty acid (NEFA), HbA1c, and body weight were comparable between the two diet groups. Postprandial glucose and insulin were higher in the standard diet group, while postprandial NEFA and triglyceride were higher in the high fat diet group, compared with the other group. The number of monocyte adherent to endothelial cells was higher in the high-fat diet group than the standard diet group. Consumption of high-fat diet resulted in overexpression of heme oxygenase-1, intercellular adhesion molecule-1 (ICAM-1), and connecting segment-1 fibronectin on the arterial wall, compared with standard diet. Thus, our data demonstrated that short-term intermittent high-fat diet prevented postprandial hyperglycemia in a model of type 2 diabetes without a significant increase in body weight. However, the resulting postprandial hypertriglyceridemia induces more monocyte adhesion to endothelial cells than postprandial hyperglycemia. This increased monocyte adhesion is associated with the increased aortic expression of adhesion molecules such as ICAM-1, and connecting segment-1 fibronectin.
authors: Department of Medicine, Metabolism and Endocrinology, Juntendo University, School of Medicine, Tokyo, Japan.
authors: Kayoko
authors: K
authors: Motojima
authors: Kosuke
authors: K
authors: Azuma
authors: Yoshiro
authors: Y
authors: Kitahara
authors: Kyoko
authors: K
authors: Miura
authors: Tomoya
authors: T
authors: Mita
authors: Takahisa
authors: T
authors: Hirose
authors: Yoshio
authors: Y
authors: Fujitani
authors: Ryuzo
authors: R
authors: Kawamori
authors: Hirotaka
authors: H
authors: Watada
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology / Q000503:physiopathology*; D001786:Blood Glucose / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology / Q000503:physiopathology*; D004041:Dietary Fats / Q000008:administration & dosage; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000473:pathology / Q000503:physiopathology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006442:Glycated Hemoglobin / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D006943:Hyperglycemia / Q000473:pathology / Q000503:physiopathology; D015228:Hypertriglyceridemia / Q000473:pathology / Q000503:physiopathology*; D007328:Insulin / Q000097:blood; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D009000:Monocytes / Q000473:pathology / Q000502:physiology*; D010455:Peptides / Q000378:metabolism; D019518:Postprandial Period; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D006442:Glycated Hemoglobin; D051547:Heme Oxygenase-1; D007328:Insulin; D018799:Intercellular Adhesion Molecule-1; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014280:Triglycerides; C076687:CS1 peptide