Rapid detection of anti-Lu(b) with recombinant Lu(b) protein and the particle gel immunoassay.
BACKGROUND
Until now, it was not possible to identify antibodies to red blood cells (RBCs) except with pretyped RBCs. Here, a novel method with particles coated with recombinant Lu(b) protein for detection of anti-Lu(b) is described.


METHODS
Prokaryotic recombinant Lu(b) proteins were generated and coupled onto superparamagnetic particles coated with streptavidin. The coated particles were tested in the presence of different serum and plasma samples (13 anti-Lu(b), 6 anti-Lu(a), 20 other antibodies, and 35 serum samples from blood donors) with the particle gel immunoassay (ID-PaGIA).


RESULTS
Lu(b)-coated particles reacted with all 13 samples containing anti-Lu(b), but not with any samples lacking anti-Lu(b). In addition, the anti-Lu(b) titers were higher with Lu(b)-coated particles than with Lu(a-b+) RBCs in almost all cases.


CONCLUSIONS
Recombinant blood group proteins may be able to dispense with the need for RBCs for identification of certain RBC alloantibodies.
Seltsam
Axel
A
Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany. seltsam.axel@mh-hannover.de
Agaylan
Ashraf
A
Grueger
Daniela
D
Meyer
Oliver
O
Blasczyk
Rainer
R
Salama
Abdulgabar
A
D007118:Immunoassay / Q000379:methods*; D007518:Isoantibodies / Q000097:blood*; D008188:Lutheran Blood-Group System / Q000276:immunology; D011387:Prokaryotic Cells / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology*; D015203:Reproducibility of Results
D007518:Isoantibodies; D008188:Lutheran Blood-Group System; D011994:Recombinant Proteins