title: Biosensing approach for glutathione detection using glutathione reductase and sulfhydryl oxidase bienzymatic system.
abstract: Chitosan membrane with glutathione reductase and sulfhydryl oxidase (SOX) was subsequently integrated onto the surface of spectrographic graphite rods for obtaining a glutathione biosensor. The working principle was based on the monitoring of O(2) consumption that correlates the concentration of glutathione during the enzymatic reaction. A linear relationship between sensor response and concentration was obtained between 0.5 and 2.0 mM for oxidized glutathione (GSSG), and 0.2-1.0 mM for reduced glutathione (GSH) in the presence of 2 microM nicotinamide adenine dinucleotide phosphate (NADPH) under the optimum working conditions. Also, reduced/oxidized glutathione were separated by HPLC and utility of bienzymatic system was investigated as an electrochemical detector for the analysis of these compounds. All data were given as a comparison of two systems: biosensor and diode array detector (DAD).
authors: Ege University, Faculty of Science, Biochemistry Department, 35100 Bornova, Izmir, Turkiye.
authors: Suna
authors: S
authors: Timur
authors: Dilek
authors: D
authors: Odaci
authors: Ayse
authors: A
authors: Dincer
authors: Figen
authors: F
authors: Zihnioglu
authors: Azmi
authors: A
authors: Telefoncu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004800:Enzymes, Immobilized; D005978:Glutathione / Q000032:analysis*; D019803:Glutathione Disulfide; D005980:Glutathione Reductase / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors
chemical_list: D004800:Enzymes, Immobilized; D005978:Glutathione; D019803:Glutathione Disulfide; D005980:Glutathione Reductase; D010088:Oxidoreductases; D010100:Oxygen; D050862:Oxidoreductases Acting on Sulfur Group Donors