Analysis of Rab GTPase and GTPase-activating protein function at primary cilia.
Primary cilia are sensory structures on the cell surface whose formation requires tight integration of microtubules and polarized membrane trafficking events. Rabs are GTP-binding proteins of the Ras superfamily that control directed membrane trafficking by regulating membrane-membrane and membrane-cytoskeleton interactions. This chapter describes cell biological and biochemical methods for the analysis of Rab function and Rab GTPase-activating proteins during primary cilia formation.
Yoshimura
Shin-ichiro
S
University of Liverpool, Cancer Research Centre, Liverpool, United Kingdom.
Haas
Alexander K
AK
Barr
Francis A
FA
D018797:Cell Cycle Proteins; D002460:Cell Line; D002923:Cilia / Q000378:metabolism*; D003001:Cloning, Molecular; D020690:GTPase-Activating Proteins / Q000032:analysis*; D006360:Heat-Shock Proteins / Q000032:analysis / Q000502:physiology; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000302:isolation & purification; D020691:rab GTP-Binding Proteins / Q000032:analysis* / Q000502:physiology
D018797:Cell Cycle Proteins; C506399:EVI5 protein, human; D020690:GTPase-Activating Proteins; D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C490107:ODF2 protein, human; C119053:RABGAP1 protein, human; C082377:RAB8A protein, human; D020691:rab GTP-Binding Proteins