[Experimental study on tissue engineered blood vessel reconstruction with bionanotechnology].
OBJECTIVE
To build nano-biomimetic tissue engineered blood vessel (NBTEBV) with nanotopology by using electrospinning (ELSP) technology.


METHODS
Cony vascular endothelial cell (VEC) on tubiform tooting in vitro was cultured. NBTEBV was built by use of multi-row nozzle with the suspension of cony vascular smooth muscle cell (VSMC) and mimic ECM (MECM) solution. NBTEBV was cultured with bioreactor in vitro. VEC and VSMC viability and proliferation were observed with MTT; and HE staining, scanning electron microscopy (SEM) observation and biomechanical test were carried out after 24 hours of static culture and 7 days of dynamic culture.


RESULTS
After 7 days of culture, the length of NBTEBV was 57 mm, the external diameter was 4 mm and the thickness of wall was 0.4 mm. The NBTEBV's color was white and the texture was even and flexible. MTT results indicated the viability of cells cultured on NBTEBV for 7 days was normal (8.9 x 10(6)/mg, 3.5 x 10(5)/mg for 24 hours). SEM and HE staining indicated that the topologic character of NBTEBV was similar to that of the natural blood vessel. The NBTEBV showed a network scaffolds structure with 100 nm thick fiber and 600 nm aperture. The HE staining result showed that the NBTEBV was composed of VEC and VSMC by layer. Vascular mechanical results showed that the NBTEBV ultimate hydrostatic pressure was 950 mmHg, the compliance of the NBTEBV under physio-pressure (110/70 mmHg) was 3.0%; the ultimate tensile strength of 20 mm x 5 mm tissue slice was 18.5 MPa.


CONCLUSIONS
The technology of ELSP can use VSMC and MECM scaffold simultaneously to build tissue engineered blood vessel with nanotopology mimic native blood vessel.
Zheng
Xiaobing
X
Department of Vascular Surgery, Affiliated Drum Tower Hospital of Najing University Medical College, Nanjing Jiangsu, 210008, P.R. China.
Qiao
Tong
T
Ran
Feng
F
Liu
Changjian
C
D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D019149:Bioreactors; D001807:Blood Vessel Prosthesis; D049109:Cell Proliferation; D002478:Cells, Cultured; D004548:Elasticity; D004730:Endothelium, Vascular / Q000166:cytology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D036103:Nanotechnology / Q000379:methods; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials