Investigating the involvement of host factors involved in intracellular pathogen infection by RNAi in Drosophila cells.
Intracellular pathogens represent a serious threat to human health. Although the biology and the virulence factors involved in intracellular bacterial infection are relatively well documented, little is known about the host factors involved in the infection process. This situation is mainly due to the difficulty of conducting extensive genetic analyses in the targeted host cells and points to the need for developing genetic systems to model the infection process. Here, we describe a method (amenable to high-throughput analysis) that allows for the study of host factors involved in intracellular pathogen infection by using dsRNA-mediated gene expression knockdown [RNA interference (RNAi)] in Drosophila cell lines.
Agaisse
Hervé
H
Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, CT, USA.
D000818:Animals; D002460:Cell Line; D004331:Drosophila melanogaster / Q000166:cytology* / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D042541:Intracellular Space / Q000382:microbiology*; D008087:Listeria / Q000502:physiology; D008088:Listeriosis / Q000382:microbiology; D008856:Microscopy, Fluorescence; D008967:Molecular Biology / Q000379:methods*; D012313:RNA / Q000302:isolation & purification; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012330:RNA, Double-Stranded; D012313:RNA