Transgenic RNA interference in mice.
RNA interference (RNAi) is a powerful tool in deciphering gene function. It has been used extensively, especially in cultured mammalian cells. We have shown that RNAi-induced gene silencing can be generated in mice. With conventional transgenic techniques, shRNA-expressing constructs can be introduced into one-cell mouse embryos. The transgenic animals so obtained exhibit reduced expression of the targeted genes. Furthermore, the knockdown effect can be transmitted through the germline in these animals. We describe a method of generating a transgenic RNAi mouse line.
Zhang
Pumin
P
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA.
D000818:Animals; D050761:Cyclin-Dependent Kinase Inhibitor p57 / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D012313:RNA / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference
C493965:Cdkn1c protein, mouse; D050761:Cyclin-Dependent Kinase Inhibitor p57; D049452:Green Fluorescent Proteins; D012313:RNA