Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation.
OBJECTIVE
To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion.


METHODS
The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.


RESULTS
p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.


CONCLUSIONS
p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
Fei
Rui
R
Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Fei
Yu
Y
Zheng
Sheng
S
Gao
Yan-guang
YG
Sun
Hong-xia
HX
Zeng
Xian-lu
XL
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D020441:Ginkgo biloba / Q000276:immunology* / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects*; D019007:P-Selectin / Q000276:immunology* / Q000378:metabolism; D018515:Plant Leaves / Q000276:immunology* / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D014471:Umbilical Veins / Q000166:cytology
D019007:P-Selectin; D011134:Polysaccharides