Biosynthesis of 15NL-phenylalanine by phenylalanine ammonia-lyase from Rhodotorula glutinis.
Catalyzed by phenylalanine ammonia-lyase from Rhodotorula glutinis, 2% trans-cinnamic acid and 0.5 mol/l (15NH4)2SO4 was bioconverted to 15NL-phenylalanine. The yield and the purity of 15NL-phenylalanine reached 71 and 99.3%, respectively. The results showed that 96% of 15N was labeled on the L-phenylalanine and 88% of (15NH4)2SO4 was recovered. The present paper provides a new and economic way for biosynthesis of 15NL-phenylalanine.
Wang
Wenya
W
State Key Laboratory of Chemical Resource Engineering, Beijing 100029, China.
Yue
Haiyan
H
Yuan
Qipeng
Q
Wang
Wenchuan
W
D002384:Catalysis; D009587:Nitrogen Isotopes / Q000737:chemistry; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D011849:Radioactive Tracers; D012248:Rhodotorula / Q000201:enzymology*
D009587:Nitrogen Isotopes; D011849:Radioactive Tracers; D010649:Phenylalanine; D010650:Phenylalanine Ammonia-Lyase