Advantages of hydride generation interface for selenium speciation in waters by high performance liquid chromatography-inductively coupled plasma mass spectrometry coupling.
This paper focuses on the analytical performance improvement of the coupled technique HPLC-ICPMS using on-line collision/reaction cell technology for selenium elemental and speciation analyses at the ng (Se) l(-1) level in aquatic environment. Collision/reaction cell operating parameters were optimised, resulting in selected conditions of 5.5 ml min(-1) H(2) and 0.5 ml min(-1) He mixture. The detection limits obtained were around 5 ng (Se) l(-1) for total analysis, and between 7 and 15 ng (Se) l(-1) depending on the species for speciation analysis. The capability of UV irradiation-hydride generation interfacing to increase detector sensitivity was also evaluated for speciation analysis. The detection limits obtained were in the range 2-8 ng (Se) l(-1) depending on the species. Moreover, such interface allowed to prevent bromine introduction to the ICPMS which is particularly convenient for selenium trace analysis in natural waters as (80)Se is preserved free from BrH interferences. The developed method was validated using certified water with low selenium content (TM Rain 95, NWRI, Canada) and applied to the analysis of different waters.
Darrouzès
Jérôme
J
Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, Université de Pau et des Pays de l'Adour, UMR CNRS 5254, Hélioparc Pau-Pyrénées, 2 avenue du Président Pierre Angot, 64053 PAU Cedex, France.
Bueno
Maïté
M
Simon
Stéphane
S
Pannier
Florence
F
Potin-Gautier
Martine
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays