Micro-flow injection system for the urinary protein assay.
A urinary protein assay has been investigated, employing a micro-flow injection analysis (muFIA) combined with an adsorptive separation of protein from analyte. The adsorptive separation part of protein in the artificial urine with ceramic hydroxyapatite is integrated on the muFIA chip, since the interference of other components coexisting in urine occurs in the conventional FIA system. The typical FI peak can be obtained following the adsorption-elution process of the protein prior to the detection, and the protein concentration in artificial urine can be quantitatively determined.
Nishihama
Syouhei
S
Department of Chemical Engineering, Faculty of Environmental Engineering, The University of Kitakyushu, Hibikino 1-1, Kitakyushu 808-0135, Japan.
Imabayashi
Hisano
H
Matoba
Tomoko
T
Toya
Chika
C
Watanabe
Kosuke
K
Yoshizuka
Kazuharu
K
D000327:Adsorption; D017886:Durapatite; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D046208:Microchip Analytical Procedures; D011506:Proteins / Q000032:analysis*; D011507:Proteinuria / Q000175:diagnosis*
D011506:Proteins; D017886:Durapatite