Conditional transgene and gene targeting methodologies in zebrafish.
The zebrafish has become a powerful tool for dissecting vertebrate gene function during embryogenesis. Numerous molecular systems have been developed to examine gene function in zebrafish, including transgenics for creating lineage-tracer lines of zebrafish that express a fluorescent protein as a marker for specific populations of cells, and antisense strategies, primarily morpholinos, for knocking down gene function. The focus of this review is to summarize the pros and cons of the currently available systems for functional genomics in zebrafish, and to discuss the need for future methodologies.
Deiters
Alexander
A
Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.
Yoder
Jeffrey A
JA