A simple and effective system for foreign gene expression in plants via root absorption of agrobacterial suspension.
Due to the laborious and scale-up limitation we have developed a simple system named "root absorption" to express foreign proteins in plants successfully. It has been shown that GFP was expressed in tobacco plants by root absorbing the Agrobacterium suspension containing TMV-based P35S-30B-GFP vector. Various factors influencing the gene expression were studied including Agrobacterium cell density, seedling age, plant materials and inoculation conditions. This system has the special advantages as simple and convenient work process, ease to scale-up and higher level of expression than leaf infiltration. Interestingly, GFP was expressed at 24h post-absorption. We assume that the root absorption system will facilitate the large-scale production of the recombinant pharmaceutical proteins in plants by means of transient expression.
Yang
Liping
L
The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
Wang
Hongwei
H
Liu
Jingying
J
Li
Liang
L
Fan
Yajun
Y
Wang
Xiufeng
X
Song
Yehua
Y
Sun
Shaoguang
S
Wang
Lei
L
Zhu
Xiaojuan
X
Wang
Xingzhi
X
D000042:Absorption; D001692:Biological Transport; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000493:pharmacokinetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000382:microbiology; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D013535:Suspensions / Q000493:pharmacokinetics; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000235:genetics; D019076:Transgenes
D013535:Suspensions; D049452:Green Fluorescent Proteins