title: Tissue-specific variation in expression of prolactin receptor gene subtypes in hypergravity-exposed rats.
abstract: Prolactin (PRL) effects are mediated by membrane receptors (PRLR) of which the long (PRLR-L) and short form (PRLR-S) predominate. Our objective was to compare the distribution pattern of PRLR-L and PRLR-S transcripts and their ratio in adipose (AD), liver (LV), mammary (MG) and pituitary (PG) tissues of stationary (SC, n = 8) and hypergravity (HG, n = 8) exposed periparturient rats. Pregnant rats were exposed to 2 g force from day 11 of gestation (G11) through post partum day 1 (P1). PRLR-L mRNA expression compared to PRLR-S was greater (P < 0.001) in AD, MG and PG but was lower (P < 0.001) in LV in both HG and SC animals at P1. The ratio of PRLR-L/PRLR-S mRNA in the AD, LV, MG and PG was not different between HG and SC rats. In summary, these data reveal that the hypergravity-induced downregulation of PRLR is not directly triggered by deranged distribution of PRLR isoforms.
authors: Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA.
authors: Osman V
authors: OV
authors: Patel
authors: R L
authors: RL
authors: Maple
authors: L A
authors: LA
authors: Baer
authors: A
authors: A
authors: Ronca
authors: C E
authors: CE
authors: Wade
authors: K
authors: K
authors: Plaut
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002498:Centrifugation; D005260:Female; D005786:Gene Expression Regulation; D018471:Hypergravity; D008099:Liver / Q000737:chemistry; D008321:Mammary Glands, Animal / Q000737:chemistry; D010902:Pituitary Gland / Q000737:chemistry; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011981:Receptors, Prolactin / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D011981:Receptors, Prolactin