title: Determination of chloride, chlorate and perchlorate by PDMS microchip electrophoresis with indirect amperometric detection.
abstract: In this work, chloride, chlorate and perchlorate are fast separated on PDMS microchip and detected via in-channel indirect amperometric detection mode. With PDMS/PDMS microchip treated by oxygen plasma, anions chloride (Cl-), chlorate (ClO3-), and perchlorate (ClO4-) are separated within 35s. Some parameters including buffer salt concentration, buffer pH, separation voltage and detection potential are investigated in detail. The separation conditions using 15 mM (pH 6.12) of 2-(N-morpholino)ethanesulfonic acid (MES)+L-histidine (L-His) as running buffer, -2000 V as separation voltage and 0.7 V as detection potential are optimized. Under this condition, the detection limits of Cl-, ClO3-, and ClO4- are 1.9, 3.6, and 2.8 microM, respectively.
authors: Key Lab of Analytical Chemistry for Life Sciences (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors: Xin-Ai
authors: XA
authors: Li
authors: Dong-Mei
authors: DM
authors: Zhou
authors: Jing-Juan
authors: JJ
authors: Xu
authors: Hong-Yuan
authors: HY
authors: Chen