title: Low-level laser therapy (670 nm) on viability of random skin flap in rats.
abstract: This study investigated the effects of 670 nm laser, at different fluences, on the viability of skin flap in rats. One hundred male animals were used. The animals were divided into control group; group treated with 3 J/cm(2); group treated with 6 J/cm(2); group treated with 12 J/cm(2) and group treated with 24 J/cm(2). The skin flap was made on the backs of all animals studied, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was done immediately after the surgery and on days 1, 2, 3 and 4 after surgery. The percentage of necrosis of the flap was calculated at the 7th postoperative day. Additionally, a sample of each flap was collected to enable us to count the blood vessels. Treated animals showed a statistically significant smaller area of necrosis than did the control group. The necrosis in the treated groups was 41.82% (group 2), 36.51% (group 3), 29.45% (group 4) and 20.37% (group 5). We also demonstrated that laser irradiation at 670 nm, at all doses used, had a stimulatory effect on angiogenesis. Our study showed that the 670 nm laser was efficient to increase the viability of the skin flap, at all fluences used, with a tendency of reaching better results at higher doses.
authors: Department of Physiotherapy, Federal University of São Carlos, Sao Carlos, SP, Brazil.
authors: Paulo Sérgio
authors: PS
authors: Bossini
authors: Renan
authors: R
authors: Fangel
authors: Rafael Malfará
authors: RM
authors: Habenschus
authors: Ana Claudia
authors: AC
authors: Renno
authors: Benedito
authors: B
authors: Benze
authors: José Antônio
authors: JA
authors: Zuanon
authors: Carlos Benatti
authors: CB
authors: Neto
authors: Nivaldo Antonio
authors: NA
authors: Parizotto
mesh_terms: D000818:Animals; D062109:Dermatologic Surgical Procedures; D004307:Dose-Response Relationship, Radiation; D054023:Lasers, Semiconductor; D028022:Low-Level Light Therapy; D008297:Male; D011879:Radiotherapy Dosage; D051381:Rats; D012867:Skin / Q000473:pathology / Q000528:radiation effects*; D013524:Surgical Flaps; D013536:Suture Techniques; D014022:Tissue Survival / Q000528:radiation effects*