Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus.
Prunus necrotic ringspot virus (PNRSV) is distributed worldwide, but no molecular data have been previously reported from South American isolates. The nucleotide sequences corresponding to the movement (MP) and coat (CP) proteins of 23 isolates of PNRSV from Chile, Brazil, and Uruguay, and from different Prunus species, have been obtained. Phylogenetic analysis performed with full-length MP and CP sequences from all the PNRSV isolates confirmed the clustering of the isolates into the previously reported PV32-I, PV96-II and PE5-III phylogroups. No association was found between specific sequences and host, geographic origin or symptomatology. Comparative analysis showed that both MP and CP have phylogroup-specific amino acids and all of the motifs previously characterized for both proteins. The study of the distribution of synonymous and nonsynonymous changes along both open reading frames revealed that most amino acid sites are under the effect of negative purifying selection.
Fiore
Nicola
N
Facultad de Ciencias Agronómicas, Universidad de Chile, Avenida Santa Rosa, 11315 Santiago, Chile.
Fajardo
Thor V M
TV
Prodan
Simona
S
Herranz
María Carmen
MC
Aparicio
Frederic
F
Montealegre
Jaime
J
Elena
Santiago F
SF
Pallás
Vicente
V
Sánchez-Navarro
Jesús
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D019179:Ilarvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D053830:Plant Viral Movement Proteins / Q000235:genetics*; D027861:Prunus / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012641:Selection, Genetic; D017386:Sequence Homology, Amino Acid; D013020:South America
D036022:Capsid Proteins; D017931:DNA Primers; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral