title: [Expression of human serum albumin in metylotrophic yeast Pichia pastoris and its structural and functional analysis].
abstract: The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity.
authors: T V
authors: TV
authors: Bobik
authors: I I
authors: II
authors: Vorob'ev
authors: N A
authors: NA
authors: Ponomarenko
authors: A G
authors: AG
authors: Gabibov
authors: A I
authors: AI
authors: Miroshnikov
mesh_terms: D002384:Catalysis; D002942:Circular Dichroism; D006801:Humans; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D012709:Serum Albumin