PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever.
PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.
Levy
Haim
H
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
Diallo
Souleymane
S
Tennant
Sharon M
SM
Livio
Sofie
S
Sow
Samba O
SO
Tapia
Milagritos
M
Fields
Patricia I
PI
Mikoleit
Matthew
M
Tamboura
Boubou
B
Kotloff
Karen L
KL
Lagos
Rosanna
R
Nataro
James P
JP
Galen
James E
JE
Levine
Myron M
MM
D000293:Adolescent; D000942:Antigens, Bacterial / Q000235:genetics; D016470:Bacteremia / Q000382:microbiology; D001769:Blood / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D005798:Genes, Bacterial; D006801:Humans; D010284:Paratyphoid Fever / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012482:Salmonella paratyphi A / Q000235:genetics / Q000302:isolation & purification*; D012484:Salmonella paratyphi B / Q000235:genetics / Q000302:isolation & purification*; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014435:Typhoid Fever / Q000175:diagnosis* / Q000382:microbiology
D000942:Antigens, Bacterial