Expression and immunogenic comparison of VP2 and VP3 from marine birnavirus.
The coding regions for the major epitopes of structural protein VP2 (vp2e) and structural protein VP3 were amplified from marine birnavirus (MABV) cDNA and efficiently expressed as glutathione S-transferase (GST) fusion proteins in Escherichia coli. Polyclonal antibodies against VP2e and VP3 were raised in rabbits and fish using the purified proteins of GST/VP2e and GST/VP3. The rabbit anti-serum against VP3 was more sensitive than the rabbit anti-VP2e serum in detecting virus in MABV-infected fish, while fish anti-VP2e serum showed a stronger neutralization response than fish anti-VP3 serum.
Xu
H-J
HJ
College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
Lin
J-G
JG
Zhang
C-X
CX
Suzuki
S
S
D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D018059:Birnaviridae / Q000235:genetics / Q000276:immunology*; D018175:Birnaviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology*; D015967:Gene Expression Regulation, Viral / Q000276:immunology*; D005982:Glutathione Transferase / Q000235:genetics; D007106:Immune Sera / Q000378:metabolism; D007668:Kidney / Q000821:virology; D036441:Osmeriformes / Q000821:virology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D005982:Glutathione Transferase