Implication of mast cell degranulation in ischemic preconditioning-induced prevention of cerebral injury.
The present study has been designed to pharmacologically investigate the role of mast cell degranulation in ischemic preconditioning-induced reversal of global ischemia- and reperfusion-induced cerebral injury in mice. Bilateral carotid artery occlusion of 17 min followed by reperfusion for 24 h was employed in present study to produce ischemia- and reperfusion-induced cerebral injury in mice. Cerebral infarct size was measured using triphenyltetrazolium chloride staining. Memory was evaluated using Morris water-maze test. Rota-rod test was employed to assess motor incoordination. Bilateral carotid artery occlusion followed by reperfusion produced cerebral infarction and impaired memory and motor coordination. Three preceding episodes of bilateral carotid artery occlusion for 1 min and reperfusion of 1 min (ischemic preconditioning) prevented markedly ischemia-reperfusion-induced cerebral injury measured in terms of infarct size, loss of memory and motor coordination. Sodium cromoglycate (10 mg/kg, i.p.), a mast cell stabilizer attenuated the neuroprotective effect of ischemic preconditioning. It is concluded that neuroprotective effect of ischemic preconditioning may be due to the degranulation of mast cells.
Rehni
Ashish K
AK
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.
Bhateja
Pradeep
P
Singh
Nirmal
N
Jaggi
Amteshwar S
AS
D000818:Animals; D002545:Brain Ischemia / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D016893:Carotid Stenosis / Q000150:complications; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology*; D002544:Cerebral Infarction / Q000473:pathology; D004205:Cromolyn Sodium / Q000494:pharmacology; D005260:Female; D019194:Ischemic Preconditioning; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D018782:Maze Learning; D051379:Mice; D011597:Psychomotor Performance; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*
D004205:Cromolyn Sodium