title: Radical scavenging, antibacterial, and antiproliferative activities of Melissa officinalis L. extracts.
abstract: The aromatic herb Melissa officinalis L. can be used as an easily accessible source of natural antioxidants and as a possible food supplement and as a phytochemical. Radical scavenging, antibacterial, and antiproliferative activities of petroleum ether, chloroform, ethyl acetate, n-butanol, and water extracts of M. officinalis L. extracts were investigated. The results of antioxidative activity, obtained by electron spin resonance spectroscopy, confirmed that investigated extracts suppressed the formation of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and lipid peroxyl radicals in all investigated systems in a dose-dependent manner. The maximum DPPH and hydroxyl radical scavenging activities (SA(DPPH) = SA(OH) = 100%) were achieved in the presence of n-butanol extract at concentrations of 0.4 mg/mL and 0.5 mg/mL, respectively. The highest lipid peroxyl scavenging activity (93.20%) was observed at a higher concentration (5 mg/mL) of n-butanol extract in the lipid peroxidation system. The most effective antibacterial activities were expressed by petroleum ether and ethyl acetate extracts on Sarcina lutea. Chloroform extract showed the strongest antiproliferative effect with 50% inhibitory concentration values of 0.09 mg/mL and 0.10 mg/mL for HeLa and MCF-7 cell lines, respectively. The present study demonstrated the high phenolic content and radical scavenging, antibacterial, and antiproliferative activities of extracts of M. officinalis L. originating from Serbia.
authors: Facultie of Technology, University of Novi Sad, Novi Sad, Serbia. jasnab@uns.ns.ac.yu
authors: Jasna
authors: J
authors: Canadanović-Brunet
authors: Gordana
authors: G
authors: Cetković
authors: Sonja
authors: S
authors: Djilas
authors: Vesna
authors: V
authors: Tumbas
authors: Gordana
authors: G
authors: Bogdanović
authors: Anamarija
authors: A
authors: Mandić
authors: Sinisa
authors: S
authors: Markov
authors: Dragoljub
authors: D
authors: Cvetković
authors: Vladimir
authors: V
authors: Canadanović
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds; D001943:Breast Neoplasms; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D017665:Hydroxyl Radical; D015227:Lipid Peroxidation / Q000187:drug effects; D031334:Melissa / Q000737:chemistry*; D010545:Peroxides; D010636:Phenols / Q000032:analysis; D010851:Picrates; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010545:Peroxides; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C049375:perhydroxyl radical; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl