title: Histological evaluation of biocompatibility of lyophilized bovine pericardium implanted subcutaneously in rats.
abstract: This article aims at investigating in vivo evaluation of lyophilization procedure on the biocompatibility of bovine pericardium treated with glutaraldehyde (GA). The bovine pericardium was fixed with 0.5% glutaraldehyde during 10 days and preserved in 4% formaldehyde (FA). Two groups of samples were prepared from treated membranes: Group 1, nonlyophilized samples and Group 2, lyophilized samples. Male Sprague-Dawley rats (4 weeks after birth) were anesthetized (pentobarbital sodium 25 mg/kg of body weight) and in each one were implanted subcutaneously in the dorsal region a sample from Group 1 and another from Group 2. These samples were explanted after 30 days for histological analysis. No intercurrences took place after the surgery. No differences (P > 0.05) in the calcification, granulomatous reaction, mononuclear infiltration, and granulation tissue development was observed between both groups. The implanted lyophilized samples presented a trend for a reduced inflammatory reaction. Lyophilization of the bovine pericardium does not seem to increase the above listed tissue reaction.
authors: Bioengineering Division-Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil. biomarina@incor.usp.br
authors: Marina J S
authors: MJ
authors: Maizato
authors: Mara D
authors: MD
authors: Pires
authors: Mauro
authors: M
authors: Canzian
authors: Olga Z
authors: OZ
authors: Higa
authors: Ronaldo N M
authors: RN
authors: Pitombo
authors: Adolfo A
authors: AA
authors: Leirner
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002114:Calcinosis / Q000473:pathology; D002417:Cattle; D002921:Cicatrix / Q000473:pathology; D003432:Cross-Linking Reagents / Q000737:chemistry; D005612:Freeze Drying; D005976:Glutaral / Q000737:chemistry; D006097:Granulation Tissue / Q000473:pathology; D015745:Granuloma, Foreign-Body / Q000473:pathology; D006350:Heart Valve Prosthesis; D008297:Male; D008422:Materials Testing; D010496:Pericardium / Q000473:pathology / Q000637:transplantation*; D011474:Prosthesis Design; D051381:Rats; D017207:Rats, Sprague-Dawley; D040521:Subcutaneous Tissue / Q000473:pathology / Q000601:surgery*; D013997:Time Factors; D016707:Tissue Fixation; D014021:Tissue Preservation / Q000379:methods; D014183:Transplantation, Heterologous
chemical_list: D003432:Cross-Linking Reagents; D005976:Glutaral