In vitro haemolytic and cytotoxic activity of soluble extract antigen of T. vaginalis isolates from symptomatic and asymptomatic women.
Human Trichomonas vaginalis infection ranges from asymptomatic to mild, moderate or severe clinical manifestations. The reasons for diverse symptomatology have been found to vary in several attributes and both parasite and host factors appear to play a role in the pathogenesis. The present study reports the in vitro haemolytic and cytotoxic activity of crude soluble extract (CSE) antigen of T. vaginalis isolates from 15 symptomatic and 15 asymptomatic women. The haemolytic activity following the interaction of CSE antigen with human erythrocytes and cytotoxic activity by adding CSE antigen to normal human vaginal epithelial cells was significantly higher with the use of CSE antigen of isolates from symptomatic women as compared to those from asymptomatic women. Furthermore, cytotoxic effect was found to be pH dependent. The study demonstrates, for the first time, the significant effect of parasite antigen of isolates from symptomatic women as compared to those from asymptomatic women in inducing haemolytic and cytotoxic effect and supports the earlier report that contact-independent mechanism(s) may be playing a role in establishing symptomatic trichomoniasis.
Malla
Nancy
N
Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India. drnancymalla@yahoo.com
Kaul
Praneet
P
Sehgal
Rakesh
R
Gupta
Indu
I
D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification / Q000633:toxicity*; D002353:Carrier State / Q000469:parasitology*; D002457:Cell Extracts / Q000302:isolation & purification / Q000633:toxicity*; D004847:Epithelial Cells / Q000187:drug effects*; D005260:Female; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014247:Trichomonas Vaginitis / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000737:chemistry* / Q000302:isolation & purification
D000953:Antigens, Protozoan; D002457:Cell Extracts