title: Detection of siRNAs and miRNAs.
abstract: Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.
authors: Wilhelm Johannsen Center for Functional Genome Research, Department of Cellular and Molecular Medicine, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
authors: Sakari
authors: S
authors: Kauppinen
authors: Zoltán
authors: Z
authors: Havelda
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D020868:Gene Silencing; D017403:In Situ Hybridization / Q000379:methods; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D015345:Oligonucleotide Probes; D010942:Plant Viruses / Q000235:genetics*; D010944:Plants / Q000821:virology; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics
chemical_list: D035683:MicroRNAs; D015345:Oligonucleotide Probes; D034741:RNA, Small Interfering; D012367:RNA, Viral