Crosstalk between integrin and G protein pathways involved in mechanotransduction in mandibular condylar chondrocytes under pressure.
To investigate the role of integrin and G protein pathways in the mechanotransduction process within MCCs and explore the possible crosstalk between the two traditional signal pathways, in vitro-cultured rabbit MCCs were treated with pressure. The mRNA level of alpha5beta1 integrin was determined by in situ hybridization and the distributions of vinculin, Galphaq/11 protein, F-actin and intracellular calcium were studied with a laser scanning confocal microscope. Increased integrin alpha5beta1 expression, enhanced stress fiber assembly, elevated G protein and vinculin level and up-regulated IP(3) channel sensitivity were found in the mechanotransduction process of MCCs under pressure. Furthermore, the vinculin and the Galphaq/11 were observed co-localized with each other, and the F-actin reassembly and stress fibers formation could be inhibited by intracellular calcium channel blocking, which gave direct evidence that the traditional integrin-mediated or G protein-mediated signaling pathways coordinately regulate the function of MCCs under mechanical stimulation.
Zhang
Min
M
Department of General Dentistry & Emergency, School of Stomatology, Fourth Military Medical University, No. 145 Changle West Road, Xi'an 710032, China. zhangmin@fmmu.edu.cn
Chen
Yong-Jin
YJ
Ono
Takahiro
T
Wang
Jing-Jie
JJ
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D019902:Chondrocytes / Q000378:metabolism*; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D008334:Mandible / Q000166:cytology / Q000378:metabolism*; D040542:Mechanotransduction, Cellular; D011312:Pressure; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D016596:Vinculin / Q000378:metabolism
D017931:DNA Primers; D016023:Integrins; D012333:RNA, Messenger; D016596:Vinculin; D019204:GTP-Binding Proteins