Distribution of Salmonella pathogenicity island (SPI)-8 and SPI-10 among different serotypes of Salmonella.
Many virulence phenotypes of Salmonella enterica are encoded by genes located on pathogenicity islands. Based on genome analysis, it is predicted that Salmonella pathogenicity island (SPI)-8 is restricted to Salmonella serovars Typhi and Paratyphi A, and SPI-10 to Salmonella serovars Typhi, Paratyphi, Enteritidis, Dublin and Gallinarum. This study was conducted to investigate the distribution of SPI-8 and SPI-10 among Salmonella isolates from sprouts, fish, water and blood. A total of 110 Salmonella isolates and 6 Salmonella serovars from the Microbial Type Culture Collection, Chandigarh, India, were screened. All isolates belonging to Salmonella serovars Washington, Enteritidis and Paratyphi A had both SPI-8 and SPI-10. All Salmonella serovar Typhi isolates from water and blood had both SPI-8 and SPI-10, whereas isolates from fish contained only SPI-8. SPI-8 and SPI-10 were also detected in only 3 out of 42 isolates belonging to Salmonella serovar Typhimurium. Both SPI-8 and SPI-10 were absent in Salmonella serovars Worthington, Dublin, Paratyphi B and Paratyphi C. These results contradict the predictions from Salmonella genome sequences available in GenBank and indicate that SPI-8 and SPI-10 are widely distributed among Salmonella serovars and that virulence factors other than those on SPI-8 and SPI-10 may be responsible for host specificity. This is the first report on the distribution of SPIs in Salmonella isolates from India.
Saroj
Sunil D
SD
Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
Shashidhar
R
R
Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
Karani
Manisha
M
Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
Bandekar
Jayant R
JR
Food Technology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India.
D000818:Animals; D015373:Bacterial Typing Techniques; D001769:Blood / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D044404:Genomic Islands / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D012480:Salmonella Infections / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013045:Species Specificity; D014675:Vegetables / Q000382:microbiology; D014774:Virulence; D014871:Water Microbiology