title: Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica.
abstract: Complete sequences of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna are presented. In particular, small subunit (18S) and internal transcribed spacers (ITS1 and ITS2) of the ribosomal gene (rDNA), as well as cytochrome c oxidase subunit I (cox1) and nicotinamide dehydrogenase subunit I (nad1) of the mitochondrial DNA (mtDNA), were analyzed. The 18S and ITS sequences were compared with previously published sequences of the liver fluke Fasciola hepatica. Fixed interspecific genetic differences were determined that allow molecular differentiation of F. magna and F. hepatica using either the PCR-RFLP method or PCR amplification of species-specific DNA regions. Additionally, intraspecific sequence polymorphism of the complete cox1 and nad1 mitochondrial genes in geographically distinct F. magna populations was determined. Based on the sequence divergences, short (< 500 bp) variable regions suitable for broader biogeographical studies of giant liver fluke were designed.
authors: Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Kosice, Slovakia. hromadova@saske.sk
authors: Ivica
authors: I
authors: Králová-Hromadová
authors: Marta
authors: M
authors: Spakulová
authors: Eva
authors: E
authors: Horácková
authors: Ludmila
authors: L
authors: Turceková
authors: Adam
authors: A
authors: Novobilský
authors: Relja
authors: R
authors: Beck
authors: Bretislav
authors: B
authors: Koudela
authors: Albert
authors: A
authors: Marinculić
authors: Dusan
authors: D
authors: Rajský
authors: Margo
authors: M
authors: Pybus
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D051545:Cyclooxygenase 1 / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D003670:Deer; D005210:Fasciola hepatica / Q000145:classification / Q000235:genetics*; D005212:Fasciolidae / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation; D008099:Liver / Q000469:parasitology; D008969:Molecular Sequence Data; D009243:NAD / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary
chemical_list: D018509:DNA, Helminth; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D009243:NAD; D051545:Cyclooxygenase 1