Immunochemical methods for the rapid screening of the o-glycosidically linked N-acetylglucosamine modification of proteins.
for the rapid screening of specific post-translational modifications antibody-based methods are very well suited and applicable without demanding expenditure. Here we describe the immunochemical detection of the O-glycosidically linked cytosolic N-acetylglucosamine modification of proteins, which has attracted increasing interest in the last years. Two different monoclonal antibodies were used in enzyme-linked immunosorbent assays (ELISA), Western blots of 1- and 2- dimension (1D and 2D) separated proteins and immunohistochemical analysis of tissue sections. Slight differences in the recognition of this post-translational epitope by the 2 antibodies are observed and will be discussed.
Ahrend
Michael
M
Department of Biochemistry, Institute of Animal Sciences, University of Bonn, Bonn, Germany.
Käberich
Angela
A
Fergen
Marie-Therese
MT
Schmitz
Brigitte
B
D000117:Acetylglucosamine / Q000737:chemistry*; D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006027:Glycosides / Q000737:chemistry*; D007150:Immunohistochemistry; D051379:Mice; D011506:Proteins / Q000737:chemistry*
D006027:Glycosides; D011506:Proteins; D000117:Acetylglucosamine