title: Development of qualitative and semiquantitative immunoassay-based rapid strip tests for the detection of T-2 toxin in wheat and oat.
abstract: Novel qualitative as well as semiquantitative rapid strip tests for screening of T-2 mycotoxin in agricultural commodities were developed. Colloidal gold particles were coated with monoclonal anti-T-2 antibodies and used as detector reagent, indicating the strip test results by formation of up to two colored lines in a competitive assay format. The test line comprises a protein conjugate of the T-2 mycotoxin and the control line an antispecies-specific antibody to confirm the correct test development. To perform the test, 5 g of sample was extracted in a ratio of 1:5 with methanol/water (70:30) by shaking for 3 min and the extract directly used without further cleanup steps. The T-2 toxin lateral flow device (LFD) presented has a cutoff level around 100 microg/kg for naturally contaminated wheat and oat. The semiquantitative test may be used in the lower micrograms per kilogram range and allows for rapid semiquantitative photometric classification of the level of sample contamination. For both tests, results were obtained within 4 min. The developed LFDs therefore allow for the first time fast and on-site screening for the determination of T-2 toxin in cereals.
authors: Christian Doppler Laboratory for Mycotoxin Research, Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, 3430 Tulln, Austria.
authors: Alexandra
authors: A
authors: Molinelli
authors: Karina
authors: K
authors: Grossalber
authors: Manuela
authors: M
authors: Führer
authors: Sabine
authors: S
authors: Baumgartner
authors: Michael
authors: M
authors: Sulyok
authors: Rudolf
authors: R
authors: Krska
mesh_terms: D018554:Avena / Q000737:chemistry*; D004355:Drug Stability; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D011934:Reagent Strips; D015203:Reproducibility of Results; D013605:T-2 Toxin / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*
chemical_list: D011934:Reagent Strips; D013605:T-2 Toxin