Turnover of ubiquinone-0 at the acceptor side of photosynthetic reaction center.
The steady-state operation of photosynthetic reaction center from Rhodobacter sphaeroides was investigated by measuring the rate of cytochrome photo-oxidation under intensive continuous illumination (808 nm, 5 W cm(-2)). The native quinone UQ10 in Q(B) binding site of the reaction center was substituted by tailless UQ0 and the binding parameters and the turnover rate of the UQ0 was studied to test the recently discovered light-intensity dependent acceptor side effect (Gerencsér and Maróti 2006). The binding parameters of UQ0 (k(on) = 2.1 x 10(5) M(-1) s(-1) and k(off) = 100 s(-1)) were characteristic to the RC exposed to high light-intensity. The dissociation constant (K (D) = 480 microM) determined under high light intensity is 2-3 times larger than that determined from flash-experiments. The light-intensity dependent acceleration of cytochrome turnover measured on reaction center of inhibited proton binding was independent of the type of the quinone and was sensitive only to the size ("pressure") of the quinone pool. The dissociation constants of different types of semiquinones show similarly high (several orders of magnitude) increase in the modified conformation of the Q(B) binding pocket due to high intensity of illumination. This result indicates the exclusive role of the quinone headgroup in the binding of semiquinone to different conformations of the protein.
Gerencsér
László
L
Department of Biophysics, University of Szeged, Egyetem utca 2, Szeged, 6722, Hungary. glaszlo@sol.cc.u-szeged.hu
Maróti
Péter
P
D016227:Benzoquinones / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D003580:Cytochromes / Q000378:metabolism; D003902:Detergents / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008027:Light; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D011522:Protons; D012242:Rhodobacter sphaeroides / Q000201:enzymology*; D012995:Solubility; D013379:Substrate Specificity; D014451:Ubiquinone / Q000737:chemistry
D016227:Benzoquinones; D003580:Cytochromes; D003902:Detergents; D045322:Photosynthetic Reaction Center Complex Proteins; D011522:Protons; D014451:Ubiquinone; C109863:ubiquinone-O; C025204:Ubiquinone Q2