title: A simple and effective system for foreign gene expression in plants via root absorption of agrobacterial suspension.
abstract: Due to the laborious and scale-up limitation we have developed a simple system named "root absorption" to express foreign proteins in plants successfully. It has been shown that GFP was expressed in tobacco plants by root absorbing the Agrobacterium suspension containing TMV-based P35S-30B-GFP vector. Various factors influencing the gene expression were studied including Agrobacterium cell density, seedling age, plant materials and inoculation conditions. This system has the special advantages as simple and convenient work process, ease to scale-up and higher level of expression than leaf infiltration. Interestingly, GFP was expressed at 24h post-absorption. We assume that the root absorption system will facilitate the large-scale production of the recombinant pharmaceutical proteins in plants by means of transient expression.
authors: The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
authors: Liping
authors: L
authors: Yang
authors: Hongwei
authors: H
authors: Wang
authors: Jingying
authors: J
authors: Liu
authors: Liang
authors: L
authors: Li
authors: Yajun
authors: Y
authors: Fan
authors: Xiufeng
authors: X
authors: Wang
authors: Yehua
authors: Y
authors: Song
authors: Shaoguang
authors: S
authors: Sun
authors: Lei
authors: L
authors: Wang
authors: Xiaojuan
authors: X
authors: Zhu
authors: Xingzhi
authors: X
authors: Wang
mesh_terms: D000042:Absorption; D001692:Biological Transport; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000493:pharmacokinetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000382:microbiology; D012231:Rhizobium / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D013535:Suspensions / Q000493:pharmacokinetics; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000235:genetics; D019076:Transgenes
chemical_list: D013535:Suspensions; D049452:Green Fluorescent Proteins