Differentiation of CD133-positive pancreatic cells into insulin-producing islet-like cell clusters.
Adult pancreatic stem and progenitor cells could represent an alternative source of insulin-producing tissue for diabetes treatment. In order to identify these cells, we have focused on the human pancreatic cells expressing cell surface molecule CD133, a marker of adult stem cells. We found that population of human CD133-positive pancreatic cells contains endocrine progenitors expressing neurogenin-3 and cells expressing human telomerase, ABCG2, Oct-3/4, Nanog, and Rex-1, markers of pluripotent stem cells. These cells were able to differentiate into insulin-producing cells in vitro and secreted C-peptide in a glucose-dependent manner. Based on our results, we suppose that the CD133 molecule represents another cell surface marker suitable for identification and isolation of pancreatic endocrine progenitors.
Koblas
T
T
Laboratory of Pancreatic Islets, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Pektorova
L
L
Zacharovova
K
K
Berkova
Z
Z
Girman
P
P
Dovolilova
E
E
Karasova
L
L
Saudek
F
F
D000071916:AC133 Antigen; D015703:Antigens, CD / Q000032:analysis*; D002096:C-Peptide / Q000032:analysis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000502:physiology; D008280:Magnetics; D010179:Pancreas / Q000166:cytology*; D010455:Peptides / Q000032:analysis*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000071916:AC133 Antigen; D015703:Antigens, CD; D002096:C-Peptide; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D012313:RNA