13 Cis-retinoic acid mediates apoptosis in Dalton's lymphoma ascites cells by regulating gene expression.
Apoptosis is a form of regulated cell death that plays an important role in the maintenance of tissue homeostasis. Treatment of Dalton's lymphoma ascites cells (DLA)-induced experimental solid tumor tumor cells with 13 cis-retinoic acid, at concentrations of 25 microg/mL and 50 microg/mL, produced apoptotic morphologic changes, such as nuclear condensation and DNA-ladder formation after 48h incubation at 37 degrees C. in addition, the compound upregulated caspase-3 expression and downregulated bcl-2 gene expression. In vivo treatment with 13 cis-retinoic acid (200 micromoles/kg body weight, ip) inhibited solid tumor development induced with DLA cells in Swiss albino mice. In conclusion, the results suggest that the anti-tumor activity of 13 cis-retinoic acid in DLA cells is due to apoptosis mediated by the regulation of bcl-2 and caspase-3 gene expression.
Guruvayoorappan
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Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Thrissur 680555, Kerala state, India.
Pradeep
C R
CR
Kuttan
Girija
G
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001201:Ascites / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D015474:Isotretinoin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008223:Lymphoma / Q000473:pathology; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019253:Proto-Oncogene Proteins c-bcl-2; D053148:Caspase 3; D015474:Isotretinoin