title: Age-dependent changes in uptake and recycling of ascorbic acid in erythrocytes of Beagle dogs.
abstract: Uptake and recycling of ascorbic acid (AA) were studied in erythrocytes of 1- to 12-month-old Beagle dogs. At 1 month, both AA uptake and recycling capacity were high. Ascorbic acid entered erythrocytes mainly in the oxidized form with elevated activity of Glut 1 glucose transporter. However, this trait of erythrocytes was rapidly lost in the course of postnatal growth. At 3 months, ascorbic acid uptake and recycling capacity decreased to almost adult levels. Thereafter, AA was transported mainly in the reduced form, and its uptake and recycling capacity became one-third the levels of 1-month-old dogs. Postnatal anemia and recovery were indicated by changes in hemoglobin and packed cell volume levels at 1 and 3 months. Glutathione reductase (GR) activity was twice as high as in adults in 1-month-old dogs, allowing efficient reduction of oxidized ascorbic acid, which enters cells in large amounts due to elevated activity of the Glut 1 glucose transporter. One-month-old dogs need high levels of AA for antioxidant protection and skeletal development. The high AA recycling capacity of erythrocytes is considered to balance the expenditure of AA in young Beagle dogs.
authors: School of Veterinary Medicine, Azabu University, Fuchinobe, Sagamihara-shi, Kanagawa, Japan ogawae@azabu-u.ac.jp
authors: Eri
authors: E
authors: Ogawa
mesh_terms: D000367:Age Factors; D000375:Aging / Q000097:blood*; D000740:Anemia / Q000097:blood / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood*; D001692:Biological Transport; D003847:Deoxyglucose / Q000097:blood; D004285:Dogs; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D051272:Glucose Transporter Type 1 / Q000097:blood; D005978:Glutathione / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D006400:Hematocrit; D006454:Hemoglobins / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction
chemical_list: D051272:Glucose Transporter Type 1; D006454:Hemoglobins; D003847:Deoxyglucose; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid