title: Myosin-Va mediates RNA distribution in primary fibroblasts from multiple organs.
abstract: Myosin-Va has been shown to have multiple functions in a variety of cell types, including a role in RNA transport in neurons. Using primary cultures of cells from organs of young dilute-lethal (Myo5a(d-l)/Myo5a(d-l)) null mutant mice and wild-type controls, we show that in some, but not all, tissues, RNA distribution is dramatically different in the homozygous null mutant cells. The dependence of RNA localization on myosin-Va correlates with the relative abundance of the brain-specific splicing pattern of the myosin-Va tail. We also show that myosin-Va is involved in RNA localization soon after synthesis, because the effects of its absence are diminished for RNAs that are more than 30 min old. Finally, we show that localization of beta-actin mRNA is significantly changed by the absence of myosin-Va. These results suggest that myosin-Va is involved in a transient transport or tethering function in the perinuclear region.
authors: McLaughlin Research Institute, Great Falls, Montana 59405-4900, USA.
authors: Verônica P
authors: VP
authors: Salerno
authors: Aldo
authors: A
authors: Calliari
authors: D William
authors: DW
authors: Provance
authors: José R
authors: JR
authors: Sotelo-Silveira
authors: José R
authors: JR
authors: Sotelo
authors: John A
authors: JA
authors: Mercer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D051379:Mice; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D009928:Organ Specificity; D034443:RNA Transport; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*
chemical_list: C513082:Myo5a protein, mouse; D012333:RNA, Messenger; D024701:Myosin Type V; D018995:Myosin Heavy Chains