title: The function of three indigenous plasmids in Mesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum.
abstract: A Mesorhizobium huakuii strain 2020, isolated from a rice-growing field in southern China, contains three indigenous plasmids named p2020a, p2020b and p2020c, respectively. The plasmids were deleted via Tn5-sacB insertion, and two cured derivatives were obtained. Interestingly, the mutant 2020D29 curing of p2020c could significantly enhance the capacity of symbiotic nitrogen fixation. But the mutant 2020D8 curing of p2020b lost the ability to nodulate Astragalus sinicus. Furthermore, the third plasmid p2020a could be hardly eliminated, suggesting that some house-keeping genes necessary for strain growth located on this plasmid. Then the Sym plasmid pJB5JI of R. leguminosarum bv. viciae was transferred into 2020 and its cured derivatives. The pot plant test showed that the ability of competition and symbiotic nitrogen fixation of transconjugant 2020-137 (pJB5JI) was increased evidently in contrast to 2020. pJB5JI could not restore the ability of 2020D8 to nodulate Astragalus sinicus. 2020D8-8 (pJB5JI) could form ineffective nodules on peas, which implied that the symbiotic plasmid pJB5JI could express its function at the chromosomal background of Mesorhizobium huakuii 2020. The plasmid stability was checked in transconjugants under free-living and during symbiosis. The results indicated that pJB5JI failed to be detected in some nodule isolates. That Km resistance gene could be amplified from all transconjugants and nodule isolates suggested that pJB5JI was fully or partially integrated into the chromosome of recipients.
authors: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors: ChengYun
authors: C
authors: Yang
authors: YouGuo
authors: Y
authors: Li
authors: Li
authors: L
authors: Wei
authors: GuoJun
authors: G
authors: Cheng
authors: JunChu
authors: J
authors: Zhou
mesh_terms: D003227:Conjugation, Genetic; D007887:Fabaceae / Q000382:microbiology; D009586:Nitrogen Fixation; D018517:Plant Roots / Q000382:microbiology; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D016961:Rhizobium leguminosarum / Q000235:genetics*; D013559:Symbiosis