Construction of infectious clones of double-stranded DNA viruses of plants using citrus yellow mosaic virus as an example.
Double-stranded DNA (dsDNA) viruses of plants are believed to be plant pararetroviruses. Their genome is replicated by reverse transcription of a larger than unit-length terminally redundant RNA transcript of the viral genomic DNA using the virus-encoded replicase. In order to produce a cloned, infectious viral genome, the clone must be constructed in a binary vector and be longer than the full, unit-length viral genome. The clone can then be transferred by Agrobacterium-assisted inoculation into a suitable host plant to induce virus infection.
Huang
Qi
Q
Floral and Nursery Plants Research Unit, U.S. National Arboretum, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD, USA.
Hartung
John S
JS
D019195:Badnavirus / Q000235:genetics* / Q000472:pathogenicity*; D003001:Cloning, Molecular / Q000379:methods; D004267:DNA Viruses / Q000235:genetics* / Q000472:pathogenicity*; D004279:DNA, Viral / Q000235:genetics*; D016679:Genome, Viral; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral