Polymorphisms in the lymphotoxin alpha gene and the risk of ischemic stroke in the Japanese population. The Fukuoka Stroke Registry and the Hisayama Study.
OBJECTIVE
Lymphotoxin alpha (LTA), one of the tumor necrosis factor family proteins, is an important proinflammatory cytokine and appears to play a putative role in the inflammatory process of atherosclerosis. Recent genetic studies have suggested that variations in the gene encoding LTA, which affect its expression and biological function, may contribute to the development of vascular diseases. We conducted a case-control study to clarify the association of LTA gene polymorphisms with ischemic stroke in a large Japanese population.


METHODS
Genotyping for LTA A252G and C804A polymorphisms was achieved by a rapid-cycle polymerase chain reaction and melting curve analysis using fluorescent probes in 1,044 incident cases of ischemic stroke recruited from the Fukuoka Stroke Registry and 1,044 age- and sex-matched control subjects recruited from the Hisayama Study.


RESULTS
The overall distribution of allele and genotype for each polymorphism was similar between stroke patients and control subjects. The allele frequencies of 252G and 804A were slightly lower in stroke patients than in control subjects; however, conditional logistic regression analysis adjusted for potential risk factors found no association between the risk of ischemic stroke and either polymorphism. In terms of stroke subtype, we also found no association of these polymorphisms with any subtypes of ischemic stroke.


CONCLUSIONS
Neither the A252G nor C804A polymorphism of the LTA gene was associated with stroke overall and any subtypes of ischemic stroke in the Japanese population.
Hagiwara
Noriko
N
Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Kitazono
Takanari
T
Kamouchi
Masahiro
M
Kuroda
Junya
J
Ago
Tetsuro
T
Hata
Jun
J
Ninomiya
Toshiharu
T
Ooboshi
Hiroaki
H
Kumai
Yasuhiro
Y
Yoshimura
Sohei
S
Tamaki
Kinya
K
Fujii
Kenichiro
K
Nagao
Tetsuhiko
T
Okada
Yasushi
Y
Toyoda
Kazunori
K
Nakane
Hiroshi
H
Sugimori
Hiroshi
H
Yamashita
Yoshichika
Y
Wakugawa
Yoshiyuki
Y
Kubo
Michiaki
M
Tanizaki
Yumihiro
Y
Kiyohara
Yutaka
Y
Ibayashi
Setsuro
S
Iida
Mitsuo
M
D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002545:Brain Ischemia / Q000150:complications / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007564:Japan; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012042:Registries; D020521:Stroke / Q000208:ethnology / Q000235:genetics*
D008233:Lymphotoxin-alpha