Isolation of membrane protein complexes by blue native electrophoresis.
Blue native PAGE is a discontinuous electrophoretic system that allows the separation of membrane protein complexes in a native, enzymatically active state with high resolution. Membrane protein complexes are solubilized by neutral, nondenaturing detergents like n-dodecyl-beta-D-maltoside. After addition of Coomassie G250 that binds to the surface of the proteins, separation of the negatively charged complexes according to molecular mass is possible. After electrophoresis the structure and function of the isolated protein complexes can be investigated.
Reisinger
Veronika
V
Ludwig-Maximilians-University Munich, Munich, Germany.
Eichacker
Lutz A
LA
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005960:Glucosides / Q000737:chemistry*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D046912:Multiprotein Complexes / Q000032:analysis* / Q000737:chemistry; D012394:Rosaniline Dyes / Q000737:chemistry*
D005960:Glucosides; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D012394:Rosaniline Dyes; C040358:dodecyl maltoside; C004692:coomassie Brilliant Blue