Sendai virus F glycoprotein induces IL-6 production in dendritic cells in a fusion-independent manner.
We previously reported that inactivated Sendai virus particle (hemagglutinating virus of Japan envelope; HVJ-E) has anti-tumor effects by eliciting IL-6 production in dendritic cells (DCs). In the present study, we investigated which components of HVJ-E elicit IL-6 production. HVJ-E containing F0 protein inactive for virus envelope-cell membrane fusion enhanced IL-6 production. Reconstituted liposomes containing F protein stimulated IL-6 production. The antibody against F protein inhibited IL-6 secretion by HVJ-E. When carbohydrate chains of the F glycoprotein were removed, HVJ-E lost the ability to stimulate IL-6 secretion. These results suggest that F glycoprotein is required for IL-6 production in DCs.
Suzuki
Hayato
H
Division of Gene Therapy Science, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
Kurooka
Masayuki
M
Hiroaki
Yoko
Y
Fujiyoshi
Yoshinori
Y
Kaneda
Yasufumi
Y
D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000378:metabolism*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008561:Membrane Fusion; D051379:Mice; D014760:Viral Fusion Proteins / Q000502:physiology*
D015850:Interleukin-6; D014760:Viral Fusion Proteins