title: Staging of neurofibrillary pathology in Alzheimer's disease: a study of the BrainNet Europe Consortium.
abstract: It has been recognized that molecular classifications will form the basis for neuropathological diagnostic work in the future. Consequently, in order to reach a diagnosis of Alzheimer's disease (AD), the presence of hyperphosphorylated tau (HP-tau) and beta-amyloid protein in brain tissue must be unequivocal. In addition, the stepwise progression of pathology needs to be assessed. This paper deals exclusively with the regional assessment of AD-related HP-tau pathology. The objective was to provide straightforward instructions to aid in the assessment of AD-related immunohistochemically (IHC) detected HP-tau pathology and to test the concordance of assessments made by 25 independent evaluators. The assessment of progression in 7-microm-thick sections was based on assessment of IHC labeled HP-tau immunoreactive neuropil threads (NTs). Our results indicate that good agreement can be reached when the lesions are substantial, i.e., the lesions have reached isocortical structures (stage V-VI absolute agreement 91%), whereas when only mild subtle lesions were present the agreement was poorer (I-II absolute agreement 50%). Thus, in a research setting when the extent of lesions is mild, it is strongly recommended that the assessment of lesions should be carried out by at least two independent observers.
authors: Department of Neuroscience and Neurology, Kuopio University, Kuopio, Finland. Irina.Alafuzoff@uku.fi
authors: Irina
authors: I
authors: Alafuzoff
authors: Thomas
authors: T
authors: Arzberger
authors: Safa
authors: S
authors: Al-Sarraj
authors: Istvan
authors: I
authors: Bodi
authors: Nenad
authors: N
authors: Bogdanovic
authors: Heiko
authors: H
authors: Braak
authors: Orso
authors: O
authors: Bugiani
authors: Kelly
authors: K
authors: Del-Tredici
authors: Isidro
authors: I
authors: Ferrer
authors: Ellen
authors: E
authors: Gelpi
authors: Giorgio
authors: G
authors: Giaccone
authors: Manuel B
authors: MB
authors: Graeber
authors: Paul
authors: P
authors: Ince
authors: Wouter
authors: W
authors: Kamphorst
authors: Andrew
authors: A
authors: King
authors: Penelope
authors: P
authors: Korkolopoulou
authors: Gábor G
authors: GG
authors: Kovács
authors: Sergey
authors: S
authors: Larionov
authors: David
authors: D
authors: Meyronet
authors: Camelia
authors: C
authors: Monoranu
authors: Piero
authors: P
authors: Parchi
authors: Efstratios
authors: E
authors: Patsouris
authors: Wolfgang
authors: W
authors: Roggendorf
authors: Danielle
authors: D
authors: Seilhean
authors: Fabrizio
authors: F
authors: Tagliavini
authors: Christine
authors: C
authors: Stadelmann
authors: Nathalie
authors: N
authors: Streichenberger
authors: Dietmar R
authors: DR
authors: Thal
authors: Stephen B
authors: SB
authors: Wharton
authors: Hans
authors: H
authors: Kretzschmar
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D001921:Brain / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D018450:Disease Progression; D005260:Female; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008875:Middle Aged; D019579:Neocortex / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016874:Neurofibrillary Tangles / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D015588:Observer Variation; D058225:Plaque, Amyloid / Q000378:metabolism / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D016875:tau Proteins / Q000032:analysis / Q000378:metabolism*
chemical_list: D016875:tau Proteins