title: Quantitative determination of fluorinated caffeic acid phenethyl ester derivative from rat blood plasma by liquid chromatography-electrospray ionization tandem mass spectrometry.
abstract: The quantitative determination of caffeic acid phenethyl ester (CAPE) and its fluorinated derivative (FCAPE) from rat plasma using ultra-performance liquid chromatography with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) is reported. CAPE and FCAPE were extracted using ethyl acetate in the presence of methyl caffeate (MC) as internal standard. Separation was achieved using a C(18) column (2.1 mm x 50 mm, 1.7 microm) and gradient elution with water and acetonitrile containing 0.2% and 0.1% formic acid, respectively. A non-linear response over a broad concentration range (1-1000 ng/ml, r(2)>0.995 using a quadratic regression model and 1/concentration weighting) was obtained. The inter-day and intra-day variability for CAPE and FCAPE were found to be less than 14.2% and 9.5%, respectively. Data are presented to illustrate the practicality of the method for the pharmacokinetic evaluation of CAPE and FCAPE after intravenous administration to rats.
authors: Pharmaceutics Division, College of Pharmacy, University of Texas at Austin, Austin, TX 78712, United States.
authors: Xinyu
authors: X
authors: Wang
authors: Jihai
authors: J
authors: Pang
authors: Robert A
authors: RA
authors: Newman
authors: Sean M
authors: SM
authors: Kerwin
authors: Phillip D
authors: PD
authors: Bowman
authors: Salomon
authors: S
authors: Stavchansky
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D005461:Fluorine / Q000737:chemistry*; D008297:Male; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002109:Caffeic Acids; D005461:Fluorine; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol