Radiofungicidal effects of external gamma radiation and antibody-targeted beta and alpha radiation on Cryptococcus neoformans.
We evaluated the clonogenic survival, membrane permeability, metabolic activity (XTT reduction), and apoptosis (FLICA binding) of Cryptococcus neoformans cells subjected to gamma rays from an external source, and beta and alpha particles delivered to fungal cells by capsule-specific antibody. We found that gamma, beta, and alpha radiation affected cells through different pathways.
Bryan
Ruth A
RA
Department of Nuclear Medicine, Joint Research Centre, Institute for Transuranium Elements, Karlsruhe, Germany.
Huang
Xianchun
X
Morgenstern
Alfred
A
Bruchertseifer
Frank
F
Casadevall
Arturo
A
Dadachova
Ekaterina
E
D000512:Alpha Particles; D000818:Animals; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000528:radiation effects; D001610:Beta Particles; D001729:Bismuth; D002463:Cell Membrane Permeability / Q000528:radiation effects*; D015169:Colony Count, Microbial; D003455:Cryptococcus neoformans / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D011868:Radioisotopes; D012211:Rhenium; D013778:Tetrazolium Salts / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011868:Radioisotopes; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D012211:Rhenium; D001729:Bismuth