title: Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus bacteria in environmental waters.
abstract: An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.
authors: MLT Research Ltd., 5 Chiltern Close, Cardiff CF14 5DL, United Kingdom. ceri.morris@mltresearch.com
authors: Ceri A
authors: CA
authors: Morris
authors: A Denene
authors: AD
authors: Blackwood
authors: Marek
authors: M
authors: Kirs
authors: Neil D
authors: ND
authors: Buttigieg
authors: Rhian R
authors: RR
authors: Morgan
authors: James J
authors: JJ
authors: Hogan
authors: Ian
authors: I
authors: Weeks
authors: Rachel T
authors: RT
authors: Noble
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D016983:Enterococcus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014871:Water Microbiology