Characterization of the roles of Rac1 and Rac2 GTPases in lymphocyte development.
This chapter describes methods for the analysis of B and T lymphocyte development in mice deficient in Rac1 and/or Rac2 GTPases. The development of both B and T cells is critically dependent on transition through checkpoints monitoring for correct rearrangement of antigen receptor genes. Progression through these checkpoints depends on signaling from the antigen receptors. In addition, signals from cytokine, chemokine, Notch, and death receptors play important roles in the survival, proliferation, and migration of developing lymphocytes. Analysis of these processes in mice deficient in these GTPases can illuminate their roles in transducing signals from these different receptors.
Dumont
Celine
C
Division of Immune Cell Biology, National Institute for Medical Research, London, United Kingdom.
Henderson
Robert
R
Tybulewicz
Victor L J
VL
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002451:Cell Compartmentation; D016923:Cell Death; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D005434:Flow Cytometry; D016131:Lymphocyte Subsets / Q000166:cytology; D051379:Mice; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000502:physiology*; D020744:rac GTP-Binding Proteins / Q000172:deficiency / Q000502:physiology*; D020830:rac1 GTP-Binding Protein / Q000172:deficiency / Q000502:physiology*; D000097766:RAC2 GTP-Binding Protein
D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein; D000097766:RAC2 GTP-Binding Protein