Detection of copy number changes at multiple loci in DNA prepared from formalin-fixed, paraffin-embedded tissue by multiplex ligation-dependent probe amplification.
With the increasing knowledge on the genetic alterations associated with various cancers, molecular analysis of these alterations on formalin-fixed paraffin-embedded tissue is of increasing importance. Multiplex ligation-dependent probe amplification (MLPA) is a novel technique to measure the copy number of up to 45 nucleic acid sequences in a single reaction. This method relies on sequence-specific probe hybridization to genomic DNA followed by multiplex-PCR amplification of the hybridized probe, and semi-quantitative analysis of the resulting PCR products. This method is easy to perform; requiring as little as 50 ng of DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue.
Takata
Minoru
M
Shinshu University School of Medicine, Matsumoto, Japan.
D004247:DNA / Q000235:genetics*; D019075:Electrophoresis, Capillary; D005557:Formaldehyde; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods*; D016612:Paraffin Embedding
D005557:Formaldehyde; D004247:DNA