title: Physiological heart activation by adrenaline involves parallel activation of ATP usage and supply.
abstract: During low-to-high work transition in adult mammalian heart in vivo the concentrations of free ADP, ATP, PCr (phosphocreatine), P(i) and NADH are essentially constant, in striking contrast with skeletal muscle. The direct activation by calcium ions of ATP usage and feedback activation of ATP production by ADP (and P(i)) alone cannot explain this perfect homoeostasis. A comparison of the response to adrenaline (increase in rate-pressure product and [PCr]) of the intact beating perfused rat heart with the elasticities of the PCr producer and consumer to PCr concentration demonstrated that both the ATP/PCr-producing block and ATP/PCr-consuming block are directly activated to a similar extent during physiological heart activation. Our finding constitutes a direct evidence for the parallel-activation mechanism of the regulation of oxidative phosphorylation in heart postulated previously in a theoretical way.
authors: Résonance Magnétique des Systèmes Biologiques, UMR5536 Centre National de la Recherche Scientifique, Université Victor Segalen Bordeaux 2, France.
authors: Bernard
authors: B
authors: Korzeniewski
authors: Véronique
authors: V
authors: Deschodt-Arsac
authors: Guillaume
authors: G
authors: Calmettes
authors: Jean-Michel
authors: JM
authors: Franconi
authors: Philippe
authors: P
authors: Diolez
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D002118:Calcium / Q000737:chemistry; D002395:Catecholamines / Q000378:metabolism; D004837:Epinephrine / Q000737:chemistry / Q000378:metabolism*; D006321:Heart / Q000502:physiology*; D007477:Ions; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010725:Phosphocreatine / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002395:Catecholamines; D007477:Ions; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium; D004837:Epinephrine