Progress in allergy signal research on mast cells: the role of histamine in goblet cell hyperplasia in allergic airway inflammation - a study using the Hdc knockout mouse.
Although histamine is a central mediator in the immediate allergic reaction, its role in goblet cell hyperplasia in the airway of asthma is not completely understood. This study was designed to examine the role of histamine in goblet cell hyperplasia using histamine-deficient mice (Hdc(-/-) mice) with allergic airway inflammation. Wild-type and Hdc(-/-) C57BL/6 mice were sensitized with ovalbumin (OVA). After two-week exposure to OVA, goblet cell hyperplasia was evaluated. Cell differentials in BALF were analyzed. The mRNAs level of MUC5AC and Gob-5 gene were quantitatively determined. The number of eosinophils in BALF increased in both the wild-type mice and Hdc(-/-) mice; however, their ratio in Hdc(-/-) mice was significantly lower than that in the wild-type mice. The mRNA levels of Gob-5 and MUC5AC and the ratio of the goblet cells in the airway epithelium were significantly increased in Hdc(-/-) mice exposed to OVA compared to the wild-type mice under the same condition. These results suggested that histamine may play a regulatory role in goblet cell hyperplasia in allergic airway inflammation.
Yamauchi
Kohei
K
Third Department of Internal Medicine, Iwate Medical University School of Medicine, Japan. kyamauch@iwate-med.ac.jp
Piao
Hong Mei
HM
Nakadate
Toshihide
T
Shikanai
Toshiki
T
Nakamura
Yutaka
Y
Niisato
Miyuki
M
Ito
Harumasa
H
Mouri
Takashi
T
Kobayashi
Hitoshi
H
Maesawa
Chihaya
C
Sawai
Takashi
T
Ohtsu
Hiroshi
H
Inoue
Hiroshi
H
D000818:Animals; D001249:Asthma / Q000473:pathology*; D018118:Chloride Channels / Q000235:genetics; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D020397:Goblet Cells / Q000473:pathology*; D006632:Histamine / Q000502:physiology*; D006640:Histidine Decarboxylase / Q000502:physiology*; D006965:Hyperplasia; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055271:Mucin 5AC; D009077:Mucins / Q000235:genetics; D009088:Mucoproteins / Q000235:genetics; D010047:Ovalbumin / Q000276:immunology
D018118:Chloride Channels; C116464:Clca3a1 protein, mouse; D016207:Cytokines; C524679:Muc5ac protein, mouse; D055271:Mucin 5AC; D009077:Mucins; D009088:Mucoproteins; D006632:Histamine; D010047:Ovalbumin; D006640:Histidine Decarboxylase