The editing enzyme ADAR1 and the mRNA surveillance protein hUpf1 interact in the cell nucleus.
Posttranscriptional regulation is an important step in the regulation of gene expression. In this article, we show an unexpected connection between two proteins that participate in different processes of posttranscriptional regulation that ensures the production of functional mRNA molecules. Specifically, we show that the A-to-I RNA editing protein adenosine deaminase that acts on RNA 1 (ADAR1) and the human Upf1 (hUpf1) protein involved in RNA surveillance are found associated within nuclear RNA-splicing complexes. A potential functional role for this association was revealed by RNAi-mediated down-regulation of ADAR1, which was accompanied by up-regulation of a number of genes previously shown to undergo A-to-I editing in Alu repeats and to be down-regulated by hUpf1. This study suggests a regulatory pathway by a combination of ADAR1 A-to-I editing enzyme and RNA degradation presumably with the aid of hUpf1.
Agranat
Lily
L
Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Raitskin
Oleg
O
Sperling
Joseph
J
Sperling
Ruth
R
D000243:Adenosine Deaminase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D011485:Protein Binding; D017393:RNA Editing / Q000235:genetics*; D020365:RNA Helicases; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D003432:Cross-Linking Reagents; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D015534:Trans-Activators; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase; D020365:RNA Helicases; C101828:UPF1 protein, human