Nisin adsorption to hydrophobic surfaces coated with the PEO-PPO-PEO triblock surfactant Pluronic F108.
The adsorption and elution of the antimicrobial peptide nisin at hydrophobic, silanized silica surfaces coated with the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) surfactant Pluronic F108 were measured in situ, with ellipsometry. While such layers are known to inhibit protein adsorption, nisin was observed to adsorb in multilayer quantities, to an extent similar to its adsorption at uncoated, hydrophobic surfaces. The rates of nisin adsorption and elution were generally slower at F108-coated surfaces. And, the sequential adsorption of nisin, including two adsorption-elution cycles at each surface, showed greater differences in adsorption rates between the first and second adsorption cycles, when evaluated at identical mass density, for uncoated relative to F108-coated surfaces. These results indicate that nisin adsorption occurs via "entrapment" within the PEO brush layer at F108-coated surfaces, in this way slowing adsorption and spontaneous elution, and inhibiting post-adsorptive molecular rearrangements by reducing the lateral mobility of nisin. While F108-coated layers rejected adsorption of serum albumin, sequential adsorption experiments carried out with nisin and albumin showed a low level of albumin adsorption when nisin was present at the interface.
Tai
Yuan-Ching
YC
Department of Chemical Engineering, Oregon State University, Corvallis, OR 97331, USA.
Joshi
Pranav
P
McGuire
Joseph
J
Neff
Jennifer A
JA
D000327:Adsorption; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008969:Molecular Sequence Data; D009561:Nisin / Q000737:chemistry* / Q000494:pharmacology; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; C116176:PEO-PPO-PEO; D011409:Propylene Glycols; D012821:Silanes; D013501:Surface-Active Agents; D020442:Poloxamer; D009561:Nisin; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols