CagA and VacA Helicobacter pylori antibodies in gastric cancer.
BACKGROUND
Infection with different genotypes of virulent Helicobacter pylori strains (cytotoxin-associated gene A [CagA]- and/or vacuolating cytotoxin A [VacA]-positive) can play a role in the development of atrophic gastritis, duodenal ulcer (DU) and gastric cancer (GC).


OBJECTIVE
To determine whether patients with GC and H pylori-negative histological staining had previously been infected with H pylori CagA- and/or VacA-positive virulent strains.


METHODS
Twenty-three GC patients with a mean (+/- SD) age of 68.14+/-9.8 years who tested H pylori-negative on histological staining took part in the study. Three control groups were included. The first group comprised 19 patients with past H pylori infection and DUs eradicated 10 years earlier, with a mean age of 58+/-18.2 years. H pylori-negative status for this group was determined every year with Giemsa staining, and follow-up testing occured 120+/-32 months (mean +/- SD) after therapy. The subsequent control groups included 20 asymptomatic children, with a mean age of 7+/-4.47 years, and with H pylori-negative fecal tests; the final group contained 30 patients without clinical symptoms of H pylori infection, with a mean age of 68+/-11.6 years, who tested H pylori-negative by histological staining.


RESULTS
Prevalence of CagA and VacA seropositivity, respectively was 82.6% and 73.91% in GC patients; 84.2% and 84.2% in H pylori-negative DU patients; 25% and 5% in H pylori-negative children; and 36.6% and 16.6% in the patients without clinical symptoms on histological staining. CagA and VacA antibody positivity was not significantly different between GC patients and patients with DUs that had been eradicated 10 years earlier. Significant positivity was found between the children's group and the H pylori-negative (with past DUs) group (P<0.001). A statistically significant difference was found in age between groups (P<0.03).


CONCLUSIONS
Patients with GC, even when H pylori-negative at the time of the present study, may have been infected by H pylori before the onset of the disease, as confirmed by CagA and VacA seropositivity. These data reinforce the hypothesis that H pylori may be a direct carcinogenic agent of GC.
Suriani
R
R
Gastroenterology Clinical Unit, Gastroenterologia ed Endoscopia Digestiva, Università di Modena e Reggio Emilia, Modena, Italy.
Colozza
M
M
Cardesi
E
E
Mazzucco
D
D
Marino
M
M
Grosso
S
S
Sanseverinati
S
S
Venturini
I
I
Borghi
A
A
Zeneroli
M Luisa
ML
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D001399:Azure Stains; D001426:Bacterial Proteins / Q000276:immunology*; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology; D006801:Humans; D007156:Immunologic Memory / Q000502:physiology; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000276:immunology* / Q000382:microbiology*; D014774:Virulence
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001399:Azure Stains; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori