title: Increased prostaglandin H synthase-1 expression and activity level in stably Harvey-ras transfected C3H10T1/2 cells.
abstract: In these studies, prostaglandin H synthase activity was increased in stably Harvey-ras transfected C3H10T1/2 cells. The level of total prostaglandin H synthase protein was two-fold higher in stably Harvey-ras transfected C3H10T1/2 cells than in control cells with no difference in prostaglandin H synthase-2 level. Prostaglandin H synthase-1 mRNA level was two-fold higher in transfected than in control cells, while prostaglandin H synthase-2 was not significantly different. Thus, prostaglandin H synthase-1, but not prostaglandin H synthase-2, expression was increased in Harvey-ras transfected C3H10T1/2 cells.
authors: Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907-1264, USA.
authors: X
authors: X
authors: Xu
authors: J R
authors: JR
authors: Burgess
authors: D
authors: D
authors: Teegarden
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D011905:Genes, ras / Q000502:physiology*; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
chemical_list: D012333:RNA, Messenger; D051545:Cyclooxygenase 1