Reduced effects of spherical aberration on penetration depth under two-photon excitation.
We compare the effects of spherical aberration on the penetration depth of single-photon and two-photon excitation for instances in which the aberration is caused by the refractive-index mismatch when a beam is focused through an interface. It is shown both theoretically and experimentally that two-photon fluorescence imaging experiences less spherical aberration and can thus propagate to a deeper depth within a thick medium.
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Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, PO Box 218, Hawthorn 3122, Victoria, Australia.
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