Inhibition of ICAM2 induces radiosensitization in oral squamous cell carcinoma cells.
We recently identified genes and molecular pathways related to radioresistance of oral squamous cell carcinoma (OSCC) using Affymetrix GeneChip. The current study focused on the association between one of the target genes, intercellular adhesion molecule 2 (ICAM2), and resistance to X-ray irradiation in OSCC cells, and evaluated the antitumor efficacy of combining ICAM2 small interfering RNA (siRNA) and X-ray irradiation. Downregulation of ICAM2 expression by siRNA enhanced radiosensitivity of OSCC cells with the increased apoptotic phenotype via phosphorylation (ser473) of AKT and activation of caspase-3. Moreover, overexpression of ICAM2 induced greater OSCC cell resistance to the X-ray irradiation with the radioresistance phenotype. These results suggested that ICAM2 silencing is closely related to sensitivity of OSCC cells to radiotherapy, and that ICAM2 may be an effective radiotherapeutic target for this disease.
Ishigami
T
T
Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Uzawa
K
K
Fushimi
K
K
Saito
K
K
Kato
Y
Y
Nakashima
D
D
Higo
M
M
Kouzu
Y
Y
Bukawa
H
H
Kawata
T
T
Ito
H
H
Tanzawa
H
H
D015703:Antigens, CD / Q000032:analysis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000532:radiotherapy*; D053148:Caspase 3 / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D009062:Mouth Neoplasms / Q000473:pathology / Q000532:radiotherapy*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D011836:Radiation Tolerance; D014162:Transfection
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; C065289:ICAM2 protein, human; D034741:RNA, Small Interfering; D051057:Proto-Oncogene Proteins c-akt; D053148:Caspase 3