title: Physicochemical characterisation of glycoconjugate vaccines for prevention of meningococcal diseases.
abstract: Bacterial capsular polysaccharides covalently linked to an appropriate carrier protein represent the best tool to induce a protective immune response against a wide range of bacterial diseases, such as meningococcal infections. We describe here the physico-chemical characterisation of glycoconjugate molecules designed to prepare a vaccine against Neisseria meningitidis serogroups A, C, W135 and Y. The use of a selective conjugation chemistry resulted in well characterised, reproducible and traceable glycoconjugate that can be consistently manufactured at large scale. A pool of physical and spectroscopic methods was used to establish glycosylation ratio, identity, molecular weight profiles, integrity of carrier protein and sites of glycosylation, assuring effective and consistent lots of vaccines.
authors: Novartis Vaccines and Diagnostics Srl, Via Fiorentina 1, 53100 Siena, Italy.
authors: Angela
authors: A
authors: Bardotti
authors: Giovanni
authors: G
authors: Averani
authors: Francesco
authors: F
authors: Berti
authors: Stefania
authors: S
authors: Berti
authors: Valeria
authors: V
authors: Carinci
authors: Sandro
authors: S
authors: D'Ascenzi
authors: Barbara
authors: B
authors: Fabbri
authors: Sara
authors: S
authors: Giannini
authors: Aldo
authors: A
authors: Giannozzi
authors: Claudia
authors: C
authors: Magagnoli
authors: Daniela
authors: D
authors: Proietti
authors: Francesco
authors: F
authors: Norelli
authors: Rino
authors: R
authors: Rappuoli
authors: Stefano
authors: S
authors: Ricci
authors: Paolo
authors: P
authors: Costantino
mesh_terms: D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008585:Meningitis, Meningococcal / Q000517:prevention & control*; D022401:Meningococcal Vaccines / Q000737:chemistry* / Q000592:standards*; D008958:Models, Molecular; D015394:Molecular Structure; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000592:standards
chemical_list: D022401:Meningococcal Vaccines; D018074:Vaccines, Conjugate