Influence of culture conditions on mycelial growth and bioluminescence of Gerronema viridilucens.
Herein we describe a procedure for measuring the total light emission of the naturally bioluminescent tropical fungus Gerronema viridilucens and the optimization of culture conditions using multivariate factorial anova. Cultures growing on an agar surface in 35 mm Petri dishes at 90% humidity show optimal bioluminescence emission at 25 degrees C in the presence of 1.0% sugar cane molasses, 0.10% yeast extract and pH 6.0 (nonbuffered). Temperature and pH are the most important factors for both mycelial growth and bioluminescence.
Mendes
Luiz F
LF
Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.
Bastos
Erick L
EL
Desjardin
Dennis E
DE
Stevani
Cassius V
CV
D000363:Agaricales / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D018533:Biomass; D001938:Brazil; D003470:Culture Media / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008164:Luminescent Proteins / Q000378:metabolism*; D025282:Mycelium / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D008164:Luminescent Proteins