title: Response surface methodology as an approach to determine optimal activities of lipase entrapped in sol-gel matrix using different vegetable oils.
abstract: The conditions for maximization of the enzymatic activity of lipase entrapped in sol-gel matrix were determined for different vegetable oils using an experimental design. The effects of pH, temperature, and biocatalyst loading on lipase activity were verified using a central composite experimental design leading to a set of 13 assays and the surface response analysis. For canola oil and entrapped lipase, statistical analyses showed significant effects for pH and temperature and also the interactions between pH and temperature and temperature and biocatalyst loading. For the olive oil and entrapped lipase, it was verified that the pH was the only variable statistically significant. This study demonstrated that response surface analysis is a methodology appropriate for the maximization of the percentage of hydrolysis, as a function of pH, temperature, and lipase loading.
authors: Chemical Engineering Department, State University of Maringa, Maringá, PR, Brazil.
authors: Rubiane C
authors: RC
authors: Pinheiro
authors: Cleide M F
authors: CM
authors: Soares
authors: Heizir F
authors: HF
authors: de Castro
authors: Flavio F
authors: FF
authors: Moraes
authors: Gisella M
authors: GM
authors: Zanin
mesh_terms: D000327:Adsorption; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D008049:Lipase / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D011786:Quality Control; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D010938:Plant Oils; D008049:Lipase