title: [Development of recombinant scFv-antibodies against diphtheria toxin using phage display system].
abstract: Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 x 10(7) M(-1) was selected. scFv preserved activity on storage in the course of 8 months.
authors: E S
authors: ES
authors: Oleĭnik
authors: A A
authors: AA
authors: Kaberniuk
authors: D A
authors: DA
authors: Burkaleva
authors: S I
authors: SI
authors: Romaniuk
authors: D V
authors: DV
authors: Kolibo
authors: A O
authors: AO
authors: Shepeliakovskaia
authors: A G
authors: AG
authors: Laman
authors: S V
authors: SV
authors: Komisarenko
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D004165:Diphtheria / Q000175:diagnosis / Q000276:immunology; D004167:Diphtheria Toxin / Q000276:immunology*; D004355:Drug Stability; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D004167:Diphtheria Toxin; D007135:Immunoglobulin Variable Region; D019151:Peptide Library; D011993:Recombinant Fusion Proteins