[Analysis of maltose clearance in plasma and urine of healthy volunteers with high-performance liquid chromatography].
OBJECTIVE
To analyze the maltose clearance in plasma and urine of healthy volunteers with high-performance liquid chromatography.


METHODS
Maltose solution was infused to 12 healthy volunteers during a 4-hour period at an infusion rate of 0.2, 0.3, and 0.5 g/(kg x h), Plasma and urine specimens were collected at different time points before and after infusion, and then analyzed with high-performance liquid chromatography.


RESULTS
The coefficients of variation of the precision and accuracy of the analysis method ranged 3.68%-4.58% and 0.44%-4.83% for plasma, respectively, and 2.91%-7.62% and 0.95%-8.27% for urine, respectively. The plasma maltose concentration increased in a dose-dependent manner (r > 0.99). The plasma maltose concentrations returned to the baseline levels 12 hours later. Two hours after injection, the urinary excretion of maltose increased, reached the peak value within 2-4 hours, began to decrease 6 hours later, and became zero 24 hours later.


CONCLUSIONS
An infusion rate of 0.2-0.5 g/(kg x h) of maltose will not remarkably increase the blood glucose level in healthy people. The routine infusion rate should below 0.3 g/(kg x h), unless an emergency exists.
He
Gui-Zhen
GZ
Department of Parenteral and Enteral Nutrition, PUMC Hospital, CAMS and PUMC, Beijing 100730, China. hgzpumc@163.com
Shu
Hong
H
Wang
Xiu-Rong
XR
Dong
Liang-Guang
LG
Ma
En-Ling
EL
D001786:Blood Glucose / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008320:Maltose / Q000097:blood* / Q000652:urine*
D001786:Blood Glucose; D008320:Maltose