Isolation of the islets of Langerhans from the human pancreas with magnetic retraction.
Low yield and insufficient purity limit the transplantation of human islets of Langerhans. In the rat, a new technique to isolate the islets of Langerhans was developed by intraarterial infusion of iron particles into the islet capillaries. After digestion the iron-loaded islets were purified with magnetic retraction (MR). In the present study, 10 human pancreata not suitable for clinical use were arterially injected with an iron-oxide suspension. After injection a small piece was used for histological analysis, and the tail was intraductally perfused with collagenase and manually digested. The yield was compared with 11 pancreata processed in the standard way. Nine of 10 pancreata were successfully injected with iron-oxide and digested. After MR, enrichment was achieved but the purity was not more than 50%. Similar results between the 2 groups were obtained regarding digestion times (23 +/- 1.1 vs 22.7 +/- 1.5 minutes) and purification yields (1749 +/- 502.1 vs 2111 +/- 501.8 IE/g, P = .6) for the MR vs control groups, respectively. Histological analysis revealed that 60% to 88% of the islets contained iron aggregations with substantially higher concentrations compared with the exocrine tissue. In conclusion, iron-oxide did not influence the isolation outcome before purification. Islet purification with MR gave enrichment but no pure fractions.
Töns
H A M
HA
Department of Surgery, Leiden University Medical Center, Leiden, The Netherlands. h.a.m.tons@lumc.nl
Baranski
A G
AG
Terpstra
O T
OT
Bouwman
E
E
D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation; D008280:Magnetics; D010179:Pancreas / Q000166:cytology