[Protective effect of bicyclol against acute fatty liver induced by tetracycline in mice].
To study the effect of bicyclol on lipid disorder and liver damage induced by tetracycline in mice, mice were given (ig) bicyclol (75, 150, and 300 mg x kg(-1)) three times before or after administration of tetracycline (180 mg x kg(-1)). The contents of hepatic lipids, MDA and GSH, serum lipids and ALT/AST levels were measured 24 hours after the injection (ip) of tetracycline. The beta-oxidation rate of hepatic mitochondrial fatty acid and hepatic secretion of VLDL were also observed. Bicyclol (150 and 300 mg x kg(-1)) provided significant protection against fatty liver by inhibiting the elevation of hepatic TG and CHO, adjusting abnormal serum lipids, inhibiting the elevation of serum ALT, AST, and ameliorating the severity of pathological changes. Furthermore, bicyclol significantly accelerated the VLDL (TG) secretion and reversed the impairment of mitochondrial oxidation, resulting in the lipid homeostasis. The increase of MDA formation and depletion of GSH that reflect lipid peroxidation induced by tetracycline were also inhibited by bicyclol administration. The results indicated that the hepatoprotection of bicyclol was mostly due to the improvement on lipid oxidation and transportation as well as the inhibition of lipid peroxidation in tetracycline-intoxicated mice.
Tang
Tao
T
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Li
Yan
Y
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D015243:Cholesterol, VLDL / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005234:Fatty Liver / Q000097:blood / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008930:Mitochondria, Liver / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology; D011897:Random Allocation; D013752:Tetracycline; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D001713:Biphenyl Compounds; D015243:Cholesterol, VLDL; D005227:Fatty Acids; D020011:Protective Agents; D014280:Triglycerides; D008315:Malondialdehyde; C477843:bicyclol; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D013752:Tetracycline; D005978:Glutathione