Abnormal cellular reactivity to microbial antigens in patients with uveitis.
OBJECTIVE
The purpose of the present study was to evaluate the cellular response to microbial antigens in patients with idiopathic uveitis.


METHODS
Blood lymphocytes from 31 patients with uveitis and 24 healthy controls were cultivated with microbial antigens and analyzed by flow cytometry after staining with monoclonal antibodies against CD3, CD4, and activation markers CD69 and CD25.


RESULTS
Although no difference was noted in circulating lymphocytes, the activation of T cells, detected with CD69, was higher in 24-hour blood culture from uveitis patients with Candida albicans antigen (Ca-Ag) than from controls, especially in posterior uveitis and panuveitis. Moreover, late response, detected with CD25, to different microbial antigens was higher in patient with uveitis.


CONCLUSIONS
Such results suggest the role of Ca-Ag and microbial antigens in the pathogenic mechanisms of idiopathic uveitis.
Cozon
Grégoire Jacques Noël
GJ
Laboratoire d'Immunologie, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
Mbitikon-Kobo
Florentin Martial
FM
Fatoohi
Fathi
F
Spire
Myriam
M
Grange
Jean-Daniel
JD
Kodjikian
Laurent
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D014605:Uveitis / Q000276:immunology*; D000099192:CD69 Antigens
D000942:Antigens, Bacterial; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000946:Antigens, Fungal; D000953:Antigens, Protozoan; D017252:CD3 Complex; D037181:Lectins, C-Type; D000099192:CD69 Antigens