Protein fractionation by preparative electrophoresis.
Preparative electrophoresis is a protein fractionation approach useful for the enrichment of low-abundance gene products. Preparative electrophoresis is usually performed in the PrepCell apparatus. Proteins are separated according to their size in a cylindrical gel in the presence of an ionic detergent. The method is particularly efficient for the enrichment of low-molecular-mass gene products. Preparative electrophoresis can be followed by proteomic analysis, and the proteins eluted from the preparative gel can be separated by two-dimensional gel electrophoresis and identified by mass spectrometry.
Fountoulakis
Michael
M
F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Dimitraki
Ploumisti
P
D000818:Animals; D001923:Brain Chemistry; D004586:Electrophoresis / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods
D009419:Nerve Tissue Proteins