title: Identification of Marteilia refringens infecting the razor clam Solen marginatus by PCR and in situ hybridization.
abstract: Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. It is believed to have a complex life-cycle involving several hosts. In this study, we applied molecular approaches to identify this parasite in samples of the razor clam Solen marginatus from the south west coast of Spain. We used a PCR assay to amplify a fragment of the IGS rDNA region. PCR products were sequenced and the phylogenetic affinity of the sequences was determined. In situ hybridization analysis showed tissue distribution and presence of different developmental stages of the parasite in the digestive diverticula epithelium, which suggested a true parasitism in these individuals. This is the first report of the occurrence of M. refringens in the razor clam S. marginatus in the south Atlantic. The methodology described herein may be useful for accurate identification of the parasite strain in different hosts and thus provide valuable information for marteiliosis control programmes.
authors: IFAPA Centro Agua del Pino, Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Carretera Cartaya-Punta Umbría, 21459 Huelva, Spain. ilopez@ugr.es
authors: Inmaculada
authors: I
authors: López-Flores
authors: Manuel A
authors: MA
authors: Garrido-Ramos
authors: Roberto
authors: R
authors: de la Herran
authors: Carmelo
authors: C
authors: Ruiz-Rejón
authors: Manuel
authors: M
authors: Ruiz-Rejón
authors: José I
authors: JI
authors: Navas
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis; D056890:Eukaryota / Q000235:genetics / Q000302:isolation & purification*; D017403:In Situ Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D016054:DNA, Protozoan