title: The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells.
abstract: To further understand the significance of bone as a target tissues of lead toxicity, as well as a reservoir of systemic lead, it is necessary to define the effect of lead on the calcium release activated calcium influx (CRACI) in primary cultures of human osteoblast-like cells (OLC). Pb2+ inhibited the immediate CRACI dose-dependent manner. Influx of Pb2+ into human OLC was increased dose-dependent manner. The present study demonstrates that the interference of Pb2+ with CRACI of human OLC is at least twofold: (1) the initiation of CRACI, i.e., the measurable influx of Ca2+ upon Ca2+ readdition, is partially inhibited by Pb2+ and (2) the influx of Pb2+ was enhanced after CRACI had been induced.
authors: Department of Dental Pharmacology, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Korea.
authors: Hye-Ock
authors: HO
authors: Jang
authors: Ji-Suk
authors: JS
authors: Kim
authors: Woo-Cheol
authors: WC
authors: Kwon
authors: Jeong-Kuk
authors: JK
authors: Kim
authors: Myung-Suk
authors: MS
authors: Ko
authors: Dong-Hoo
authors: DH
authors: Kim
authors: Won-Il
authors: WI
authors: Kim
authors: Young-Chan
authors: YC
authors: Jeon
authors: In-Kyo
authors: IK
authors: Chung
authors: Sang-Hun
authors: SH
authors: Shin
authors: Jin
authors: J
authors: Chung
authors: Moon-Kyung
authors: MK
authors: Bae
authors: Il
authors: I
authors: Yun
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007202:Indicators and Reagents; D007476:Ionophores / Q000494:pharmacology; D007854:Lead / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012996:Solutions; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D004791:Enzyme Inhibitors; D007202:Indicators and Reagents; D007476:Ionophores; D012996:Solutions; D007854:Lead; D000001:Calcimycin; D019284:Thapsigargin; D002118:Calcium