title: Expression and immunogenic comparison of VP2 and VP3 from marine birnavirus.
abstract: The coding regions for the major epitopes of structural protein VP2 (vp2e) and structural protein VP3 were amplified from marine birnavirus (MABV) cDNA and efficiently expressed as glutathione S-transferase (GST) fusion proteins in Escherichia coli. Polyclonal antibodies against VP2e and VP3 were raised in rabbits and fish using the purified proteins of GST/VP2e and GST/VP3. The rabbit anti-serum against VP3 was more sensitive than the rabbit anti-VP2e serum in detecting virus in MABV-infected fish, while fish anti-VP2e serum showed a stronger neutralization response than fish anti-VP3 serum.
authors: College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, China.
authors: H-J
authors: HJ
authors: Xu
authors: J-G
authors: JG
authors: Lin
authors: C-X
authors: CX
authors: Zhang
authors: S
authors: S
authors: Suzuki
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D018059:Birnaviridae / Q000235:genetics / Q000276:immunology*; D018175:Birnaviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology*; D015967:Gene Expression Regulation, Viral / Q000276:immunology*; D005982:Glutathione Transferase / Q000235:genetics; D007106:Immune Sera / Q000378:metabolism; D007668:Kidney / Q000821:virology; D036441:Osmeriformes / Q000821:virology; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; D005982:Glutathione Transferase