Surface modification of polydimethylsiloxane (PDMS) induced proliferation and neural-like cells differentiation of umbilical cord blood-derived mesenchymal stem cells.
Stem cell-based therapy has recently emerged for use in novel therapeutics for incurable diseases. For successful recovery from neurologic diseases, the most pivotal factor is differentiation and directed neuronal cell growth. In this study, we fabricated three different widths of a micro-pattern on polydimethylsiloxane (PDMS; 1, 2, and 4 microm). Surface modification of the PDMS was investigated for its capacity to manage proliferation and differentiation of neural-like cells from umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). Among the micro-patterned PDMS fabrications, the 1 microm-patterned PDMS significantly increased cell proliferation and most of the cells differentiated into neuronal cells. In addition, the 1 microm-patterned PDMS induced an increase in cytosolic calcium, while the differentiated cells on the flat and 4 microm-patterned PDMS had no response. PDMS with a 1 microm pattern was also aligned to direct orientation within 10 degrees angles. Taken together, micro-patterned PDMS supported UCB-MSC proliferation and induced neural like-cell differentiation. Our data suggest that micro-patterned PDMS might be a guiding method for stem cell therapy that would improve its therapeutic action in neurological diseases.
Kim
Sun-Jung
SJ
Adult Stem Cell Research, College of Veterinary Medicine, Seoul National University, 151-742 Seoul, Republic of Korea.
Lee
Jae Kyoo
JK
Kim
Jin Won
JW
Jung
Ji-Won
JW
Seo
Kwangwon
K
Park
Sang-Bum
SB
Roh
Kyung-Hwan
KH
Lee
Sae-Rom
SR
Hong
Yun Hwa
YH
Kim
Sang Jeong
SJ
Lee
Yong-Soon
YS
Kim
Sung June
SJ
Kang
Kyung-Sun
KS
D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014470:Umbilical Cord / Q000166:cytology*
D004129:Dimethylpolysiloxanes; C013830:baysilon