title: Gene delivery to dystrophic muscle.
abstract: Electroporation is a powerful method for gene delivery to dystrophic muscle in the mdx mouse model of Duchenne muscular dystrophy. Successful transfer of reporter and therapeutic plasmids and antisense oligonucleotides has been demonstrated. However, the efficiency falls with increasing plasmid size. Although it is unlikely that the electrotransfer approach will be useful clinically, it is an important experimental tool, particularly in testing potential immune responses to gene transfer in the absence of vector proteins.
authors: Department of Cellular and Molecular Neuroscience, Division of Neuroscience and Mental Health, Imperial College, London, United Kingdom.
authors: Kim E
authors: KE
authors: Wells
authors: Jill
authors: J
authors: McMahon
authors: Helen
authors: H
authors: Foster
authors: Aurora
authors: A
authors: Ferrer
authors: Dominic J
authors: DJ
authors: Wells
mesh_terms: D000818:Animals; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D016189:Dystrophin / Q000235:genetics / Q000378:metabolism; D053672:Electrochemotherapy / Q000379:methods*; D015870:Gene Expression; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D007273:Injections, Intramuscular; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics / Q000628:therapy; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D004274:DNA, Recombinant; D016189:Dystrophin; D001616:beta-Galactosidase