Agroinoculation: a simple procedure for systemic infection of plants with viruses.
Plant-virus interaction studies, for long, plagued by asynchronous/failed infections, have improved since the usage of Agrobacterium as a delivery agent for viral genomes. Popularly known as "agroinoculation," this method has revolutionized plant virology studies, leading to identification of viruses as casual agents of disease, viral genome mutagenesis and recombination analyses, and virus-induced gene silencing (VIGS) applications. We present here a brief overview of the recent applications of this method and a detailed protocol for agroinoculation and VIGS used in our laboratory.
Vaghchhipawala
Zarir E
ZE
Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Pky., Ardmore, OK 73401, USA.
Mysore
Kirankumar S
KS
D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D017930:Genes, Reporter; D018551:Solanum lycopersicum / Q000821:virology; D009029:Mosaic Viruses / Q000472:pathogenicity; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000472:pathogenicity*; D010944:Plants / Q000382:microbiology / Q000821:virology*; D010957:Plasmids; D034622:RNA Interference; D012367:RNA, Viral / Q000235:genetics; D012231:Rhizobium / Q000472:pathogenicity
D017931:DNA Primers; D012367:RNA, Viral