Therapeutic cloning in individual parkinsonian mice.
Cell transplantation with embryonic stem (ES) cell progeny requires immunological compatibility with host tissue. 'Therapeutic cloning' is a strategy to overcome this limitation by generating nuclear transfer (nt)ES cells that are genetically matched to an individual. Here we establish the feasibility of treating individual mice via therapeutic cloning. Derivation of 187 ntES cell lines from 24 parkinsonian mice, dopaminergic differentiation, and transplantation into individually matched host mice showed therapeutic efficacy and lack of immunological response.
Tabar
Viviane
V
Department of Neurosurgery, Sloan-Kettering Institute, 1275 York Ave, New York, New York 10065, USA. tabarv@mskcc.org
Tomishima
Mark
M
Panagiotakos
Georgia
G
Wakayama
Sayaka
S
Menon
Jayanthi
J
Chan
Bill
B
Mizutani
Eiji
E
Al-Shamy
George
G
Ohta
Hiroshi
H
Wakayama
Teruhiko
T
Studer
Lorenz
L
D000818:Animals; D001522:Behavior, Animal; D002460:Cell Line; D019976:Cloning, Organism; D004298:Dopamine / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000637:transplantation*; D051379:Mice; D020297:Mice, Congenic; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism / Q000637:transplantation; D053652:Nuclear Transfer Techniques; D020734:Parkinsonian Disorders / Q000503:physiopathology / Q000523:psychology / Q000628:therapy*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D004298:Dopamine