title: Emergence and spread of carbapenem-resistant Acinetobacter baumannii in a tertiary care hospital in Turkey.
abstract: An intensive care unit (ICU)-based OXA-23-producing multiple-drug resistant Acinetobacter baumannii (MDRAB) outbreak was detected between October 2005 and October 2006. A total of 47 patients were infected/colonized with the outbreak strain. Clinical data were available from 37 patients. The all-cause mortality rate among the patients exposed to the epidemic strain was 35% (13/37). The outbreak strain and the resistance determinants were characterized both by microbiological methods and by molecular techniques. Cloning and sequencing experiments identified ISAbaI-associated bla(oxa-23) on the chromosome. Screening of imipenem-resistant Acinetobacter isolated from the ICU during the outbreak period with PCR identified 97 isolates as positive for the ISAbaI-bla(oxa-23) structure. Pulsed-field gel electrophoresis and plasmid analyses with selected nonrepetitive isolates revealed the clonality. Disk diffusion on cloxacillin-supplemented agar media and the real-time PCR experiments showed that outbreak isolates are overexpressing the ampC enzyme. This study highlights the occurrence of OXA-23-producing and ampC-overexpressing MDRAB in ICUs.
authors: Enf. Hst & Klin. Mikr. AD, Kocaeli Universitesi, Izmit, Turkey.
authors: Meliha
authors: M
authors: Meric
authors: Murat
authors: M
authors: Kasap
authors: Gulcin
authors: G
authors: Gacar
authors: Fatma
authors: F
authors: Budak
authors: Devrim
authors: D
authors: Dundar
authors: Fetiye
authors: F
authors: Kolayli
authors: Cafer
authors: C
authors: Eroglu
authors: Haluk
authors: H
authors: Vahaboglu
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000201:enzymology / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D003428:Cross Infection / Q000453:epidemiology*; D004196:Disease Outbreaks; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007362:Intensive Care Units; D014421:Turkey / Q000453:epidemiology
chemical_list: D015780:Carbapenems