Virucidal activity of ortho-phthalaldehyde solutions against hepatitis B and C viruses.
Ortho-phthalaldehyde (OPA), a high-level disinfectant alternative to glutaraldehyde, was tested for efficacy against human hepatitis B virus (HBV) and hepatitis C virus (HCV) using surrogate animal viruses. HBV and HCV are the most prevalent human bloodborne viruses but have not yet been propagated in the laboratory. The surrogate viruses, duck hepatitis B virus (DHBV) and bovine viral diarrhea virus (BVDV), were used to assess the virucidal efficacy of OPA on HBV and HCV, respectively. After a timed exposure to the test disinfectant, the surrogate virus dried on a hard surface was neutralized and assayed to detect viable viruses using appropriate cell lines. A greater than 4-log(10) reduction in virus titer was demonstrated using dilute OPA solutions against dried DHBV and BVDV after 5 minutes of exposure at 20 degrees C. OPA was shown to be efficacious against surrogate viruses for human hepatitis B and hepatitis C virus. This is the first time that OPA efficacy has been demonstrated for HBV and HCV.
Roberts
Charles G
CG
Advanced Sterilization Products, Johnson and Johnson Company, Irvine, CA 92618, USA. crobert2@aspus.jnj.com
Chan-Myers
Harriet B
HB
Favero
Martin S
MS
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D016174:Hepacivirus / Q000187:drug effects*; D015611:Hepatitis B Virus, Duck / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects*; D010576:Pestivirus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D038301:Virus Inactivation; D009764:o-Phthalaldehyde / Q000494:pharmacology*
D000998:Antiviral Agents; D009764:o-Phthalaldehyde