title: Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus.
abstract: Prunus necrotic ringspot virus (PNRSV) is distributed worldwide, but no molecular data have been previously reported from South American isolates. The nucleotide sequences corresponding to the movement (MP) and coat (CP) proteins of 23 isolates of PNRSV from Chile, Brazil, and Uruguay, and from different Prunus species, have been obtained. Phylogenetic analysis performed with full-length MP and CP sequences from all the PNRSV isolates confirmed the clustering of the isolates into the previously reported PV32-I, PV96-II and PE5-III phylogroups. No association was found between specific sequences and host, geographic origin or symptomatology. Comparative analysis showed that both MP and CP have phylogroup-specific amino acids and all of the motifs previously characterized for both proteins. The study of the distribution of synonymous and nonsynonymous changes along both open reading frames revealed that most amino acid sites are under the effect of negative purifying selection.
authors: Facultad de Ciencias Agronómicas, Universidad de Chile, Avenida Santa Rosa, 11315 Santiago, Chile.
authors: Nicola
authors: N
authors: Fiore
authors: Thor V M
authors: TV
authors: Fajardo
authors: Simona
authors: S
authors: Prodan
authors: María Carmen
authors: MC
authors: Herranz
authors: Frederic
authors: F
authors: Aparicio
authors: Jaime
authors: J
authors: Montealegre
authors: Santiago F
authors: SF
authors: Elena
authors: Vicente
authors: V
authors: Pallás
authors: Jesús
authors: J
authors: Sánchez-Navarro
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D019179:Ilarvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D053830:Plant Viral Movement Proteins / Q000235:genetics*; D027861:Prunus / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012641:Selection, Genetic; D017386:Sequence Homology, Amino Acid; D013020:South America
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; D053830:Plant Viral Movement Proteins; D012367:RNA, Viral