Reductively labile PRINT particles for the delivery of doxorubicin to HeLa cells.
A Trojan horse PRINT particle composition was developed that incorporates a reductively labile cross-linker to achieve activated release of doxorubicin in vitro. Particles of discrete size and shape (cube side length = 2 micron) containing 30 wt % of a disulfide-based cross-linker and 2 wt % doxorubicin were synthesized. This PRINT composition was shown to release doxorubicin in response to a reducing environment as measured by flow cytometry and was found to be highly proficient at killing HeLa cells in vitro.
Petros
Robby A
RA
Department of Chemistry, University of North Carolina at Chapel Hill, Caudill Laboratories CB 3290, Chapel Hill, North Carolina 27599-3290, USA.
Ropp
Patricia A
PA
DeSimone
Joseph M
JM
D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry
D011108:Polymers; D004317:Doxorubicin