title: Amplification by long RT-PCR of near full-length norovirus genomes.
abstract: A long RT-PCR method was developed to amplify the norovirus genome. Starting from RNA extracted directly from clinical samples and using broadly reactive primers, it can generate near full-length amplicons that allow for easy determination of the near complete genomic sequence. Two norovirus isolates from Toronto, Canada, in 2002 and 2005 were sequenced. This approach will facilitate molecular epidemiology studies of noroviruses.
authors: Metabolism Research Program, Hospital for Sick Children, Toronto, Canada.
authors: Jennifer
authors: J
authors: Kostela
authors: Melissa
authors: M
authors: Ayers
authors: John
authors: J
authors: Nishikawa
authors: Lorraine
authors: L
authors: McIntyre
authors: Martin
authors: M
authors: Petric
authors: Raymond
authors: R
authors: Tellier
mesh_terms: D017250:Caliciviridae Infections / Q000821:virology; D002170:Canada; D005243:Feces / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral