Detection of Cryptosporidium parvum oocysts by dot-blotting using monoclonal antibodies to Cryptosporidium parvum virus 40-kDa capsid protein.
Monoclonal antibodies (MAb) were prepared against the 40-kDa capsid protein of Cryptosporidium parvum virus (CPV) by immunizing mice with purified recombinant CPV40 protein. In immunoblotting analysis, MAbCPV40-1 bound to a 40-kDa protein in extracts of C. parvum oocysts. This 40-kDa protein was localized in the sporozoite cytoplasm by immunofluorescence (IFA) staining with MAbCPV40-1. In a dot-blot assay, MAbCPV40-1 was capable of detecting 10(2) non-bleach-treated and 10(2)-10(3) bleach-treated C. parvum oocysts. MAbCPV40-1 was capable of detecting CPV40 antigen in both soluble and total C. parvum oocyst protein extracts, indicating a potential use for detecting this parasite in environmental samples.
Jenkins
Mark C
MC
Animal Parasitic Diseases Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA. mark.jenkins@ars.usda.gov
O'Brien
Celia N
CN
Trout
James M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D002417:Cattle; D016785:Cryptosporidium parvum / Q000302:isolation & purification* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D015151:Immunoblotting / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D033661:Oocysts / Q000821:virology; D012328:RNA Viruses / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D013559:Symbiosis
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D011994:Recombinant Proteins