Observations on Cryptosporidium life cycle stages during excystation.
Cryptosporidium parvum (HNJ-1 strain, genotype 2) merozoites were released from oocysts directly during an incubation and excystation procedure without bleach treatment. They were polymorphic, mostly spindle-shaped; others were bean shaped, actively motile, and underwent division. Merozoites survived for short time-period in an in vitro culture system, but could not be established in a subsequent cultivation effort in RPMI medium.
Karanis
Panagiotis
P
Medical and Molecular Parasitology Laboratory, Medical School, Center of Anatomy, Institute II, University of Cologne, 50937 Cologne, Germany. karanis@obihiro.ac.jp
Kimura
Akio
A
Nagasawa
Hideyuki
H
Igarashi
Ikuo
I
Suzuki
Naoyoshi
N
D000328:Adult; D000818:Animals; D016785:Cryptosporidium parvum / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008018:Life Cycle Stages; D051379:Mice; D016513:Mice, SCID; D018613:Microscopy, Confocal