title: [The effect of overdose fluoride on the expression of Cbfalpha1 in the ameloblasts of rat incisor].
abstract: OBJECTIVE
  To evaluate the effect of overdose fluoride on the expression of Cbfalpha1 in the ameloblasts of rat incisor and its correlation with dental fluorosis at protein level.
  
  
  METHODS
  Twenty Wistar rats were divided randomly into two groups, one was fed with distilled water(control group), the other was fed with distilled water of 100ppm F-(experimental group). The incisors of these rats were extracted for immunohistochemical staining eight weeks later to investigate the expression of Cbfalpha1.The data was analyzed with Axioplan 2 imaging system and SPSS10.0 software package.
  
  
  RESULTS
  Cbfalpha1 was expressed in the nuclear of ameloblasts at secretory stage, and the expression level in the experimental group was significantly higher than that in the control group(P<0.01).
  
  
  CONCLUSIONS
  Overdose fluoride causes higher expression of Cbfalpha1 in the ameloblasts at secretory stage,which may effect the mineralization and development of enamel in a certain way and leads to the abnormal development of enamel.
authors: Department of Operative Dentistry, College of Stomatology, Tongji University, Shanghai 200072, China.
authors: Li-Li
authors: LL
authors: Wang
authors: Qiang
authors: Q
authors: Wang
authors: Hong-Mei
authors: HM
authors: Wu
authors: Xiao-Ling
authors: XL
authors: Wei
authors: Na
authors: N
authors: Gao
mesh_terms: D000565:Ameloblasts / Q000187:drug effects*; D000818:Animals; D050657:Core Binding Factor alpha Subunits / Q000378:metabolism*; D005459:Fluorides / Q000633:toxicity*; D009050:Fluorosis, Dental; D007180:Incisor; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050657:Core Binding Factor alpha Subunits; C406688:core binding factor alpha; D005459:Fluorides