title: Distribution of the partitioning protein KorB on the genome of IncP-1 plasmid RK2.
abstract: The ParB family partitioning protein, KorB, of plasmid RK2 is central to a regulatory network coordinating replication, maintenance and transfer genes. Previous immunofluorescence microscopy indicated that the majority of KorB is localized in plasmid foci. The 12 identified KorB binding sites on RK2 are differentiated by: position relative to promoters; binding strength; and cooperativity with other repressors and so the distribution of KorB may be sequestered around a sub-set of sites. However, chromatin immunoprecipitation analysis showed that while RK2 DNA molecules appear to sequester KorB to create a higher local concentration, cooperativity between DNA binding proteins does not result in major differences in binding site occupancy. Thus under steady state conditions all operators are close to fully occupied and this correlates with gene expression on the plasmid being highly repressed.
authors: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors: Chung-Min
authors: CM
authors: Chiu
authors: Susan E
authors: SE
authors: Manzoor
authors: Sarah M
authors: SM
authors: Batt
authors: Sidra tul
authors: St
authors: Muntaha
authors: Lewis E H
authors: LE
authors: Bingle
authors: Christopher M
authors: CM
authors: Thomas
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D047369:Chromatin Immunoprecipitation; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D017930:Genes, Reporter; D016680:Genome, Bacterial; D047468:Immunoprecipitation; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D016958:Pseudomonas putida / Q000378:metabolism; D027981:Symporters / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C053863:KorB protein, Plasmid RK2; D027981:Symporters; C033903:xylE protein, E coli; D004247:DNA