title: A different molecular mechanism underlying antimicrobial and hemolytic actions of temporins A and L.
abstract: In this work, the naturally occurring antimicrobial peptides temporin A (TA) and L (TL) are studied by spectroscopic (CD and NMR) techniques and molecular dynamics simulation. We analyzed the interactions of TA and TL with sodium dodecyl sulfate (SDS) and dodecylphosphocholine (DPC) micelles, which mimic bacterial and mammalian membranes, respectively. In SDS, the peptides prefer a location at the micelle-water interface; in DPC, they prefer a location perpendicular to the micelle surface, with the N-terminus imbedded in the hydrophobic core. TL shows higher propensity, with respect to TA, in forming alpha-helical structures in both membrane mimetic systems and the highest propensity to penetrate the micelles. Hence, we have proposed a different molecular mechanism underlying the antimicrobial and hemolytic activities of the two peptides. We also designed new analogues of TA and TL and found interesting differences in their efficacy against microbial species and human erythrocytes.
authors: Department of Pharmaceutical and Toxicological Chemistry, University of Naples "Federico II", I-80131 Naples, Italy.
authors: Alfonso
authors: A
authors: Carotenuto
authors: Stefania
authors: S
authors: Malfi
authors: Maria Rosaria
authors: MR
authors: Saviello
authors: Pietro
authors: P
authors: Campiglia
authors: Isabel
authors: I
authors: Gomez-Monterrey
authors: Maria Luisa
authors: ML
authors: Mangoni
authors: Ludovica Marcellini Hercolani
authors: LM
authors: Gaddi
authors: Ettore
authors: E
authors: Novellino
authors: Paolo
authors: P
authors: Grieco
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D002942:Circular Dichroism; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D011506:Proteins; C104501:temporin; D010767:Phosphorylcholine; D012967:Sodium Dodecyl Sulfate; C028810:dodecylphosphocholine