Role of cytokines in mediating mechanical hypernociception in a model of delayed-type hypersensitivity in mice.
In the present study, we used the electronic version of the von Frey test to investigate the role of cytokines (TNF-alpha and IL-1beta) and chemokines (KC/CXCL-1) in the genesis of mechanical hypernociception during antigen-induced inflammation in mice. The nociceptive test consisted of evoking a hindpaw flexion reflex with a hand-held force transducer (electronic anesthesiometer) adapted with a 0.5 mm(2) polypropylene tip. The intraplantar administration of methylated bovine serum albumin (mBSA) in previously immunized (IM), but not in sham-immunized (SI) mice, induced mechanical hypernociception in a dose-dependent manner. Hypernociception induced by antigen was reduced in animals pretreated with IL-1ra and reparixin (a non-competitive allosteric inhibitor of CXCR2), and in TNF receptor type 1 deficient (TNFR1-/-) mice. Consistently, antigen challenge induced a time-dependent release of TNF-alpha, IL-1beta and KC/CXCL-1 in IM, but not in SI, mice. The increase in TNF-alpha levels preceded the increase in IL-1beta and KC/CXCL1. Antigen-induced release of IL-1beta and KC/CXCL1 was reduced in TNFR1-/- mice, and TNF-alpha-induced hypernociception was inhibited by IL-1ra and reparixin. Hypernociception induced by IL-1beta in immunized mice was inhibited by indomethacin, whereas KC/CXCL1-induced hypernociception was inhibited by indomethacin and guanethidine. Antigen-induced hypernociception was reduced by indomethacin and guanethidine and abolished by the two drugs combined. Together, these results suggest that inflammation associated with an adaptive immune response induces hypernociception that is mediated by an initial release of TNF-alpha, which triggers the subsequent release of IL-1beta and KC/CXCL1. The latter cytokines in turn stimulate the release of the direct-acting final mediators, prostanoids and sympathetic amines.
Cunha
Thiago M
TM
Department of Pharmacology, Faculty of Medicine of Ribeirão Preto University of Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil.
Verri
Waldiceu A
WA
Valério
Daniel A
DA
Guerrero
Ana T
AT
Nogueira
Luciana G
LG
Vieira
Silvio M
SM
Souza
Danielle G
DG
Teixeira
Mauro M
MM
Poole
Stephen
S
Ferreira
Sergio H
SH
Cunha
Fernando Q
FQ
D000818:Animals; D054360:Chemokine CXCL1 / Q000276:immunology; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006930:Hyperalgesia / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D006968:Hypersensitivity, Delayed / Q000235:genetics / Q000276:immunology* / Q000503:physiopathology; D018836:Inflammation Mediators / Q000494:pharmacology; D053583:Interleukin-1beta / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015213:Neuroimmunomodulation / Q000235:genetics / Q000276:immunology*; D009619:Nociceptors / Q000276:immunology*; D010147:Pain Measurement; D017288:Pain Threshold; D010812:Physical Stimulation; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
D054360:Chemokine CXCL1; D018925:Chemokines; D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D047888:Receptors, Tumor Necrosis Factor, Type I; C506236:Tnfrsf1a protein, mouse; D014409:Tumor Necrosis Factor-alpha