[Expression of human serum albumin in metylotrophic yeast Pichia pastoris and its structural and functional analysis].
The stable strain of methylotrophic yeast Pichia pastoris secreting human serum albumin into cultural medium was obtained. Optimal conditions for expression of the protein were determined. We characterized the recombinant protein by mass spectrometry and circular dichroism and analyzed its catalytic activity.
Bobik
T V
TV
Vorob'ev
I I
II
Ponomarenko
N A
NA
Gabibov
A G
AG
Miroshnikov
A I
AI
D002384:Catalysis; D002942:Circular Dichroism; D006801:Humans; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
D011994:Recombinant Proteins; D012709:Serum Albumin