title: Detection and localization of small nuclear ribonucleoproteins (snRNPs) in trypanosomatids using anti-m3G antibodies.
abstract: This work reports for the first time the identification and immunolocalization, by confocal and conventional indirect immunofluorescence, of m3G epitopes present in ribonucleoproteins of the following trypanosomatids: Trypanosoma cruzi epimastigotes of three different strains, Blastocrithidia ssp., and Leishmania major promastigotes. The identity of these epitopes and hence the specificity of the anti-m3G monoclonal antibody were ascertained through competition reaction with 7-methylguanosine that blocks the Ig binding sites, abolishing the fluorescence in all the parasites tested and showing a specific perinuclear localization of the snRNPs, which suggests their nuclear reimport in the parasites. Using an immunoprecipitation technique, it was also possible to confirm the presence of the trimethylguanosine epitopes in trypanosomatids.
authors: Departamento de Ciencias Biologicas, Faculdade de Ciencias Farmaceuticas, Universidade Estadual Paulista, Sao Paulo, Brasil.
authors: Maira Cegatti
authors: MC
authors: Bosetto
authors: Renato Arruda
authors: RA
authors: Mortara
authors: Daniela Luz
authors: DL
authors: Ambrósio
authors: Gabriela Duó
authors: GD
authors: Passerini
authors: Marco Túlio Alves
authors: MT
authors: da Silva
authors: Erica Sayuri
authors: ES
authors: Okuda
authors: Regina Maria Barretto
authors: RM
authors: Cicarelli
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000939:Epitopes / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect; D047468:Immunoprecipitation; D018613:Microscopy, Confocal; D017411:Ribonucleoproteins, Small Nuclear / Q000276:immunology / Q000302:isolation & purification*; D014351:Trypanosomatina / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D017411:Ribonucleoproteins, Small Nuclear