An analysis of long-distance water transport in the soybean stem using H215O.
The lateral water movement in the intact stem of a transpiring soybean plant was analyzed quantitatively by a real-time measurement system utilizing labeled water, H(2)(15)O and gamma ray detectors. A large volume of water escaping from xylem vessels during its transport was detected. The escape of water was not influenced by evaporation from the stem surface or mass flow in the sieve tubes. It was assumed that the total amount of water transported through xylem vessels was kept almost completely constant along the internode. As a result, most of the escaped water was found to re-enter the xylem vessels, i.e. water exchange occurred. The analysis of radiographs of tritiated water suggested that the self-diffusion effect of water was strong for lateral water movement, although another driving force besides thermal motion was included in the process, and that the process was also affected by the water permeability of the plasma membrane. An analysis based on a mathematical model showed that the net volume of water which escaped from xylem vessels was not dependent on the transpiration rate of the plant.
Ohya
Tomoyuki
T
National Institute of Radiological Sciences, Medical Imaging, 4-9-1, Anagawa Inage-ku, Chibashi, 265-8555, Japan. ohya@nirs.go.jp
Tanoi
Keitaro
K
Hamada
Yousuke
Y
Okabe
Hirotaka
H
Rai
Hiroki
H
Hojo
Junko
J
Suzuki
Kazutoshi
K
Nakanishi
Tomoko M
TM
D001692:Biological Transport; D006813:Humidity; D008954:Models, Biological; D010104:Oxygen Radioisotopes; D018547:Plant Stems / Q000378:metabolism*; D018526:Plant Transpiration; D013025:Glycine max / Q000378:metabolism*; D014867:Water / Q000378:metabolism*; D052584:Xylem / Q000378:metabolism
D010104:Oxygen Radioisotopes; D014867:Water