Characterization of chemical components in extracts from Si-wu decoction with proliferation-promoting effects on rat mesenchymal stem cells.
Si-wu decoction (SDE), a classic prescription in Traditional Chinese Medicine, has been used for the treatment of a variety of anaemia in China for centuries. In order to explore the scientific basis of the formula, we investigated the relationship between its chemical components and proliferation-promoting effects on rat marrow-derived mesenchymal stem cells (MSCs). Twenty (F-1-F-20) components were obtained and their proliferation-promoting effects on MSCs were investigated. The results showed that F-4, F-7, F-10, and F-11 stimulated the proliferation of the MSCs. The chemical components with proliferation-promoting effects on the MSCs were further identified by GC-MS, HPLC, LC-MS, and other spectra. Ligustilide (F-4) isolated from SDE showed the best proliferation-promoting effect. Palmitic acid methyl ester and stearic acid ethyl identified from F-7 and F-10 by HPLC were also confirmed to be responsible for stimulating MSC proliferation. A novel compound, 6,7-dihydroxy-3-(-prop-1-enyl)isobenzofuran-1(3H)-one, was found in the SDE for the first time by LC-MS(n), whose structure was similar to ligustilide.
Zeng
He-Ping
HP
Institute of Functional Molecule, School of Chemistry, South China University of Technology, Guangzhou 510641, China. zenghp@scnu.edu.cn
Wang
Ting-Ting
TT
Chen
Wei
W
Wang
Chun-Yan
CY
Chen
Dong-Feng
DF
Shen
Jian-Gang
JG
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D015394:Molecular Structure; D010168:Palmitates / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013228:Stearates / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
C530897:6,7-dihydroxy-3-(-prop-1-enyl)isobenzofuran-1(3H)-one; D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D010168:Palmitates; D013228:Stearates; C502769:si-wu decoction; C530910:stearic acid ethyl ester; C027820:ligustilide; C019012:methyl palmitate; D015107:4-Butyrolactone