Porcine endogenous retrovirus transmission characteristics from a designated pathogen-free herd.
Previously, a strategy for monitoring pigs intended for cell transplantation was developed and successfully applied to several representative herds in New Zealand. A better understanding of porcine viruses' epidemiology in New Zealand has been achieved, and, as a result, a designated pathogen-free (DPF) herd has been chosen as a good candidate for xenotransplantation. This herd is free of all infectious agents relevant to xenotransplantation. The presented study of pig endogenous retrovirus (PERV) transmission with cocultures in vitro has shown no evidence of PERV transmission from DPF pig tissue. Additionally, in PERV-C-positive DPF donor pigs tested, a specific locus for PERV-C present in miniature swine possibly associated with the transmission of PERV was absent. The data on PERV transmission allowed classifying the DPF potential donors as "null" or noninfectious pigs.
Garkavenko
O
O
Living Cell Technologies Ltd, Otara, Manukau, Auckland, New Zealand. ogarkavenko@Ictglobal.com
Wynyard
S
S
Nathu
D
D
Muzina
M
M
Muzina
Z
Z
Scobie
L
L
Hector
R D
RD
Croxson
M C
MC
Tan
P
P
Elliott
B R
BR
D000822:Animal Husbandry / Q000592:standards; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D020077:Endogenous Retroviruses / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D005333:Fetus; D006801:Humans; D007668:Kidney / Q000196:embryology / Q000821:virology; D008297:Male; D009520:New Zealand; D012192:Retroviridae Infections / Q000517:prevention & control / Q000635:transmission*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012449:Safety; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D013737:Testis / Q000196:embryology / Q000821:virology; D014183:Transplantation, Heterologous