Promiscuous peptides on the nontypeable Haemophilus influenzae P6 outer membrane protein.
BACKGROUND
P6 outer membrane protein is one of the candidates for a vaccine formulation against nontypeable Haemophilus influenzae (NTHi) infection. As otitis-prone children who have recurrent episodes of acute otitis media because of NTHi show an impaired immune response to P6, an innovative approach to vaccination is required to augment their immune response.


CONCLUSIONS
We previously identified human HLA-DR9-restricted T cell epitope peptide and highly immunogenic analog peptides on P6 for peptide vaccine candidates. To develop a vaccine formulation effective in the general population, we identified promiscuous T cell epitope peptides (p41-55, p71-85) on P6. In addition to stimulating with potentially promiscuous peptides (p30-44, p45-59) selected using a computer algorithm, we established peptide-specific T cell lines which respond to P6.


CONCLUSIONS
Our present results indicate that these peptides would be candidates for a widely applicable peptide vaccine formulation.
Nomura
Yuka
Y
Department of Otolaryngology-Head and Neck Surgery, Asahikawa Medical College, Midorigaoka E2-1-1-1, Asahikawa, Hokkaido 078-8510, Japan.
Abe
Yusuke
Y
Ishida
Yoshiya
Y
Kobayashi
Hiroya
H
Harabuchi
Yasuaki
Y
D000465:Algorithms; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006192:Haemophilus Infections / Q000276:immunology / Q000517:prevention & control; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000276:immunology*
D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D018073:Haemophilus Vaccines; D000949:Histocompatibility Antigens Class II; C069990:P6 outer membrane protein, Haemophilus; D010455:Peptides