Localization of leptin and leptin receptor in the bovine adenohypophysis.
The present study was carried out to detail the cellular localization of leptin (Lep) and the leptin receptor (LepR) in the bovine adenohypophysis. Lep immunoreactivity (Lep-ir) was found in about 30% of adenohypophysial cells in the gland. Immunochemistry of Lep and specific hormones using serial sections revealed that Lep-ir was present in 60.4% of somatotrophs, 15.9% of gonadotrophs, 6.5% of mammotrophs, 6.5% of thyrotrophs and 2.4% of corticotrophs. Both the common short isoform (OBRa) and the long isoform (OBRb) of LepR mRNA were expressed in the bovine adenohypophysis. LepR immunoreactivity (LepR-ir) was found in only 2.8% of the adenohypophysial cells and over 50% of LepR-ir cells were gonadotrophs, in which most of the cells were distributed in the zona tuberalis. The findings on Lep and LepR in the adenohypophysial cells indicate that Lep may regulate gonadotroph function through autocrine/paracrine pathway in the bovine adenohypophysis.
Ogasawara
Hideki
H
Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555 Sendai, Japan.
Ohwada
Shyuichi
S
Nagai
Yasuhiro
Y
Taketa
Yoshikazu
Y
Matsuzaki
Masatoshi
M
Tanaka
Sachi
S
Watanabe
Kouichi
K
Aso
Hisashi
H
Yamaguchi
Takahiro
T
D000818:Animals; D019898:Autocrine Communication / Q000235:genetics / Q000502:physiology; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D052681:Gonadotrophs / Q000378:metabolism / Q000502:physiology; D020738:Leptin / Q000235:genetics / Q000378:metabolism*; D008297:Male; D019899:Paracrine Communication / Q000235:genetics / Q000502:physiology; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution
D020738:Leptin; D012333:RNA, Messenger; D054411:Receptors, Leptin