title: Cloning and expression profiling of four antibacterial peptide genes from the bumblebee Bombus ignitus.
abstract: Four antibacterial peptide genes (apidaecin, hymenoptaecin, abaecin, and defensin) were cloned from the bumblebee Bombus ignitus, and cDNAs and their genomic structures were sequenced and characterized. Comparative analysis revealed that the four antibacterial peptides of B. ignitus had similar characteristics to other bee antibacterial peptides identified to date. The transcriptional expression profiles of the four antibacterial peptide genes in the fat body of B. ignitus workers revealed that all four antibacterial peptide genes were acutely induced in a similar manner by PBS injection or LPS stimulation, indicating that antibacterial peptides from various classes are simultaneously expressed in a single insect upon infection or injury.
authors: College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Republic of Korea.
authors: Yong Soo
authors: YS
authors: Choi
authors: Young Moo
authors: YM
authors: Choo
authors: Kwang Sik
authors: KS
authors: Lee
authors: Hyung Joo
authors: HJ
authors: Yoon
authors: Iksoo
authors: I
authors: Kim
authors: Yeon Ho
authors: YH
authors: Je
authors: Hung Dae
authors: HD
authors: Sohn
authors: Byung Rae
authors: BR
authors: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001516:Bees / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D023082:Defensins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017344:Genes, Insect; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D023181:Antimicrobial Cationic Peptides; D023082:Defensins; D019476:Insect Proteins; D012333:RNA, Messenger; C080586:hymenoptaecin protein, Apis mellifera; C061361:apidaecin