title: Surface modification of polydimethylsiloxane (PDMS) induced proliferation and neural-like cells differentiation of umbilical cord blood-derived mesenchymal stem cells.
abstract: Stem cell-based therapy has recently emerged for use in novel therapeutics for incurable diseases. For successful recovery from neurologic diseases, the most pivotal factor is differentiation and directed neuronal cell growth. In this study, we fabricated three different widths of a micro-pattern on polydimethylsiloxane (PDMS; 1, 2, and 4 microm). Surface modification of the PDMS was investigated for its capacity to manage proliferation and differentiation of neural-like cells from umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). Among the micro-patterned PDMS fabrications, the 1 microm-patterned PDMS significantly increased cell proliferation and most of the cells differentiated into neuronal cells. In addition, the 1 microm-patterned PDMS induced an increase in cytosolic calcium, while the differentiated cells on the flat and 4 microm-patterned PDMS had no response. PDMS with a 1 microm pattern was also aligned to direct orientation within 10 degrees angles. Taken together, micro-patterned PDMS supported UCB-MSC proliferation and induced neural like-cell differentiation. Our data suggest that micro-patterned PDMS might be a guiding method for stem cell therapy that would improve its therapeutic action in neurological diseases.
authors: Adult Stem Cell Research, College of Veterinary Medicine, Seoul National University, 151-742 Seoul, Republic of Korea.
authors: Sun-Jung
authors: SJ
authors: Kim
authors: Jae Kyoo
authors: JK
authors: Lee
authors: Jin Won
authors: JW
authors: Kim
authors: Ji-Won
authors: JW
authors: Jung
authors: Kwangwon
authors: K
authors: Seo
authors: Sang-Bum
authors: SB
authors: Park
authors: Kyung-Hwan
authors: KH
authors: Roh
authors: Sae-Rom
authors: SR
authors: Lee
authors: Yun Hwa
authors: YH
authors: Hong
authors: Sang Jeong
authors: SJ
authors: Kim
authors: Yong-Soon
authors: YS
authors: Lee
authors: Sung June
authors: SJ
authors: Kim
authors: Kyung-Sun
authors: KS
authors: Kang
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon