title: Osteoporotic bone formation in mice lacking tob2; involvement of Tob2 in RANK ligand expression and osteoclasts differentiation.
abstract: Mice lacking tob2, a member of the antiproliferative family genes, had decreased bone mass, and the number of osteoclasts differentiated from bone marrow cells was increased. Overexpression of Tob2 in stromal cells repressed vitamin D(3)-induced osteoclasts formation. Furthermore, expression of RANKL mRNA in stromal cells was increased in the absence of Tob2 and decreased in the presence of Tob2. Tob2 interacted with vitamin D(3) receptor (VDR), which suggests its involvement in vitamin D(3) receptor-mediated regulation of transcription. Because VDR regulates RANKL expression, our data suggest that Tob2 negatively regulates formation of osteoclasts by suppressing RANKL expression through its interaction with VDR.
authors: The Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
authors: Rieko
authors: R
authors: Ajima
authors: Toru
authors: T
authors: Akiyama
authors: Michihiko
authors: M
authors: Usui
authors: Mitsuhiro
authors: M
authors: Yoneda
authors: Yutaka
authors: Y
authors: Yoshida
authors: Takahisa
authors: T
authors: Nakamura
authors: Osamu
authors: O
authors: Minowa
authors: Masaki
authors: M
authors: Noda
authors: Sakae
authors: S
authors: Tanaka
authors: Tetsuo
authors: T
authors: Noda
authors: Tadashi
authors: T
authors: Yamamoto
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D002454:Cell Differentiation; D002522:Chlorocebus aethiops; D017931:DNA Primers; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D010010:Osteoclasts / Q000166:cytology*; D010024:Osteoporosis / Q000235:genetics* / Q000473:pathology; D053245:RANK Ligand / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018797:Cell Cycle Proteins; D017931:DNA Primers; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; C485732:Tob2 protein, mouse