[Effect of intracoronary transplantation of autologous bone marrow mononuclear cells on myocardial ischemia reperfusion injury in mini-swine model].
OBJECTIVE
To investigate the therapeutic effectiveness of intracoronary transplantation of autologous bone marrow mononuclear cells (BM-MNC) on myocardial ischemia reperfusion injury in mini-swine model.


METHODS
Myocardial ischemia reperfusion injury model was established by ligating in 16 mini-swines, which were further randomized into two groups: (3.54 +/- 0.90) x 10(8) BM-MNC was intracoronarily transplanted in BM-MNC group (n = 9), and phosphate buffer saline was intracoronarily applied in the control group (n = 7). Ultrasonic cardiograhpy, hemodynamics, neovascular density, and myocardium infarction size were evaluated before and 4 weeks after transplantation.


RESULTS
In BM-MNC group, left ventricular ejection fraction (LVEF), intra-ventricular septa, lateral wall and anterior wall, cardiac output (CO) and + dp/dt(max) had no significant differences before and 4 weeks after transplantation (P > 0.05). In the control group, LVEF, intraventricular septa, lateral wall and anterior wall, CO, and + dp/dt(max) significantly decreased 4 weeks after transplantation (P < 0.05). Left ventricular end-diastolic pressure and- dp/dt(max) had no significant differences before and after cell transplantation. Capillary density was significantly larger in the BM-MNC group than in the control group [(13.39 +/- 6.96) /HP vs. (3.50 +/- 1.90) /HP]. The percentage and size of myocardial infarction was significantly lower in the BM-MNC group than in the control group.


CONCLUSIONS
Transplantation of BM-MNC into the myocardial ischemic reperfusion-injury area can increase capillary density and decrease infarction area, and thus remarkably improve cardiac systolic function.
Li
Chong-Jian
CJ
Center of Coronary Heart Disease, Fuwai Hospital, CAMS and PUMC, Beijing 100037, China.
Gao
Run-Lin
RL
Yang
Yue-Jin
YJ
Hu
Feng-Huan
FH
Yang
Wei-Xian
WX
You
Shi-Jie
SJ
Song
Lai-Feng
LF
Ruan
Ying-Mao
YM
Qiao
Shu-Bin
SB
Chen
Ji-Lin
JL
Chen
Zai-Jia
ZJ
D000818:Animals; D016026:Bone Marrow Transplantation; D003331:Coronary Vessels; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature