The effects of tungstate on skin lesions caused by PPD in rats.
P-phenylenediamine (PPD) has been used as one of the ingredients in hair dye. The purpose of this study is to investigate the skin toxicity of PPD application in a tungstate-induced xanthine oxidase (XO) deficient animal model. PPD (2.5% PPD in 2% NH4OH) was applied to rat skin (25 mg/16.5 cm2) five times every other day in rats fed a standard diet (SD) or a tungstate supplemented diet (TD). The skin structure in the SD and the TD group was intact, whereas XO activity was not detected in the TD group during experimental periods. Furthermore, there were no differences between the SD and the TD group in dermal reactive oxygen species (ROS) scavenging enzymes. In these experimental conditions, although XO activity was not detected in the applied PPD rats fed a tungstate supplemented diet (PTD) group, it showed more severe tissue damage compared with the applied PPD rats fed a standard diet (PSD) group. In addition, the PTD group showed higher increased rates of ROS scavenging enzyme activity and lipid peroxide (LPO) content, and decreased glutathione (GSH) content than in the PSD group. In conclusion, the increase of PPD dermal toxicity in tungstate-induced XO deficient animals may be due to excessive ROS via ROS imbalance during PPD skin application.
Lee
Sang-Hee
SH
Department of Style Coordination, Kyongdo Provincial College, Yeochun 757-807, Korea.
Cho
Hyun-Gug
HG
Lee
Sang-Il
SI
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D010655:Phenylenediamines; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D012871:Skin Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D017973:Tungsten Compounds / Q000627:therapeutic use*; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D010655:Phenylenediamines; D017382:Reactive Oxygen Species; D017973:Tungsten Compounds; D005979:Glutathione Peroxidase; D014969:Xanthine Oxidase; C045951:tungstate; C029728:4-phenylenediamine