title: Occurrence and genetic analysis of picorna-like viruses infecting worker bees of Apis mellifera L. populations in Devon, South West England.
abstract: Viruses of the European honeybee, Apis mellifera L. are known to reside at low levels in colonies, typically showing no apparent signs of infection. Using reverse transcription-PCR (RT-PCR), 23 apiaries in Devon were screened for the presence of 6 honeybee viruses, with positive colonies being analysed for viral genetic diversity. Ninety-seven percent of the colonies were positive for deformed wing virus (DWV), 29% were positive for acute bee paralysis virus (ABPV) and 1.4% were positive for both sacbrood virus (SBV) and black queen cell virus (BQCV). Multiple infections were common, with 32% of the colonies were infected by more than one virus.
authors: Marine Biological Association of the United Kingdom, Citadel Hill, Plymouth, Devon PL1 2PB, UK. ancba@mba.ac.uk
authors: Andrea
authors: A
authors: Baker
authors: Declan
authors: D
authors: Schroeder
mesh_terms: D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000821:virology*; D004739:England; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D010849:Picornaviridae / Q000235:genetics*; D010850:Picornaviridae Infections / Q000453:epidemiology* / Q000662:veterinary; D012367:RNA, Viral / Q000032:analysis; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase