title: Assessment of the genetic diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans as a basis to identify putative pathogenicity genes and a type III secretion system of the SPI-1 family by multiple suppression subtractive hybridizations.
abstract: Fluorescent amplified fragment length polymorphism revealed that strains of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans are genetically distinct and can be grouped into four genetic lineages. Four suppression subtractive hybridizations were then performed to isolate DNA fragments present in these bean pathogens and absent from closely related xanthomonads. Virulence gene candidates were identified such as homologs of hemagglutinins, TonB-dependent receptors, zinc-dependent metalloproteases, type III effectors, and type IV secretion system components. Unexpectedly, homologs of the type III secretion apparatus components (SPI-1 family), usually reported in animal pathogens and insect symbionts, were also detected.
authors: UMR PaVé, INRA, 42 rue Georges Morel, 49071 Beaucouzé Cedex 1, France.
authors: Seyed Mehdi
authors: SM
authors: Alavi
authors: Saeideh
authors: S
authors: Sanjari
authors: Fabien
authors: F
authors: Durand
authors: Chrystelle
authors: C
authors: Brin
authors: Charles
authors: C
authors: Manceau
authors: Stéphane
authors: S
authors: Poussier
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001426:Bacterial Proteins / Q000235:genetics*; D016000:Cluster Analysis; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D026901:Membrane Transport Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D010802:Phylogeny; D037521:Virulence Factors / Q000235:genetics*; D014974:Xanthomonas / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D037521:Virulence Factors