Concentration and emission of airborne contaminants in a laboratory animal facility housing rabbits.
Characterization of animal housing conditions can determine the frequency of bedding and cage changes, which are not standardized from facility to facility. Rabbits produce noticeable odors, and their excreta can scald and stain cages. Our facility wanted to document measurable airborne contaminants in a laboratory rabbit room in which excreta pans were changed weekly and cages changed biweekly. Contaminants included particulate, endotoxin, ammonia, carbon dioxide, and a rabbit salivary protein as a marker for rabbit allergen. Concentrations were measured daily over a 2-wk period in a laboratory animal facility to determine whether they increased over time and on days considered to be the dirtiest. Except for ammonia, concentrations of all airborne contaminants did not differ between clean and dirty days. Concentrations were lower than occupational health exposure guidelines for all contaminants studied, including ammonia. After measurement of concentration, a model was applied to calculate mean emission factors in this rabbit room. Examples of emission factor utilization to determine airborne contaminant concentration in rabbit rooms under various environmental conditions and housing densities are provided.
Ooms
Tara G
TG
Department of Vivarial Science and Research, Tulane University, New Orleans, LA, USA. tooms@tulane.edu
Artwohl
James E
JE
Conroy
Lorraine M
LM
Schoonover
Todd M
TM
Fortman
Jeffrey D
JD
D000391:Air Microbiology; D000395:Air Pollutants, Occupational / Q000032:analysis*; D016902:Air Pollution, Indoor / Q000032:analysis*; D000485:Allergens / Q000032:analysis; D000641:Ammonia / Q000032:analysis; D000818:Animals; D000830:Animals, Laboratory; D002245:Carbon Dioxide / Q000032:analysis; D004391:Dust / Q000032:analysis; D004731:Endotoxins / Q000032:analysis; D005260:Female; D006168:Guinea Pigs; D006799:Housing, Animal; D016273:Occupational Exposure; D011817:Rabbits; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000276:immunology; D013047:Specific Pathogen-Free Organisms
D000395:Air Pollutants, Occupational; D000485:Allergens; D004391:Dust; D004731:Endotoxins; D012471:Salivary Proteins and Peptides; D002245:Carbon Dioxide; D000641:Ammonia