Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane.
In this paper, we report a new method of fabricating a high-throughput protein preconcentrator in poly(dimethylsiloxane) (PDMS) microfluidic chip format. We print a submicron thick ion-selective membrane on the glass substrate by using standard patterning techniques. By simply plasma-bonding a PDMS microfluidic device on top of the printed glass substrate, we can integrate the ion-selective membrane into the device and rapidly prototype a PDMS preconcentrator without complicated microfabrication and cumbersome integration processes. The PDMS preconcentrator shows a concentration factor as high as approximately 10(4) in 5 min. This printing method even allows fabricating a parallel array of preconcentrators to increase the concentrated sample volume, which can facilitate an integration of our microfluidic preconcentrator chip as a signal enhancing tool to various detectors such as a mass spectrometer.
Lee
Jeong Hoon
JH
Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Song
Yong-Ak
YA
Han
Jongyoon
J
D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D017736:Ion-Selective Electrodes; D013058:Mass Spectrometry / Q000379:methods; D008567:Membranes, Artificial; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
D004129:Dimethylpolysiloxanes; D008567:Membranes, Artificial