[Isolation and study of the immunobiological properties of the recombinant proteins of measles virus].
Recombinant proteins rN (nucleocapsid) and rH/Nh (hemagglutinin) of the measles virus strain NovO/96 of genotype A were obtained. The immunobiological properties of the proteins were studied in the reaction with a panel of positive and negative sera. Mice of the line Balb/C were immunized with recombinant proteins and native antigen of the measles virus strain NovO/96 in order to obtain hyperimmune serum and its analysis using ELISA (enzyme-linked immunosorbent assay) and PRN (plaque reduction neutralization). The hyperimmune serum against recombinant proteins and native antigen of the measles virus strain NovO/96 were found to be highly active in ELISA. The antibodies against the proteins rN and rH/Nh were found to be able to neutralize the virus in titer 1:13.5 and 1:22.9, respectively. The neutralization titer of the antibodies generated against native antigen of the measles virus strain NovO/96 was 1:25.7.
Kulak
M V
MV
Netesova
N A
NA
Belavin
P A
PA
Seregina
E V
EV
Ignat'ev
G M
GM
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008457:Measles / Q000235:genetics / Q000276:immunology*; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D006388:Hemagglutinins; D019590:Nucleocapsid Proteins