Fast projection matching for cryo-electron microscopy image reconstruction.
A new FFT-accelerated projection matching method is presented and tested. The electron microscopy images are represented by their Fourier-Bessel transforms and the 3D model by its expansion in spherical harmonics, or more specifically in terms of symmetry-adapted functions. The rotational and translational properties of these representations are used to quickly access all the possible 2D projections of the 3D model, which allow an exhaustive inspection of the whole five-dimensional domain of parameters associated to each particle.
Estrozi
Leandro Farias
LF
IBS, Institut de Biologie Structurale Jean-Pierre Ebel., CEA, CNRS, Université Joseph Fourier, 41 rue Jules Horowitz, F-38027 Grenoble, France. leandro.estrozi@ibs.fr
Navaza
Jorge
J
D020285:Cryoelectron Microscopy / Q000379:methods*; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008954:Models, Biological