Analysis of Rho-GTPase mimicry by a family of bacterial type III effector proteins.
Microbial pathogens may hijack the actin cytoskeleton machinery to facilitate actin-based motility, cellular invasion, and intracellular trafficking through the endocytic pathway. Of particular interest has been a large class of virulence factors collectively termed the type III "effector" proteins. Following contact with a eukaryotic cell, Gram-negative bacterial pathogens employ a dedicated protein secretion apparatus termed type III to translocate effector proteins into the cellular cytoplasm where they initiate a pathogenic response. Interestingly, these effectors can mimic the structure or function of eukaryotic signaling proteins and often target components of the actin cytoskeleton. This chapter describes methodologies to examine the cellular role of the recently defined WxxxE family of effector proteins that functionally mimic Rho family small G proteins.
Alto
Neal M
NM
Department of Microbiology, University of Texas Southwestern, Dallas, Texas, USA.
Dixon
Jack E
JE
D036002:ADP Ribose Transferases / Q000037:antagonists & inhibitors; D000577:Amides / Q000494:pharmacology; D000595:Amino Acid Sequence; D001905:Botulinum Toxins / Q000037:antagonists & inhibitors; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004843:Epistasis, Genetic; D029968:Escherichia coli Proteins / Q000502:physiology; D000080865:Formins; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000502:physiology*; D018716:Molecular Mimicry / Q000502:physiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011725:Pyridines / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D020830:rac1 GTP-Binding Protein / Q000096:biosynthesis / Q000502:physiology*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*
D000577:Amides; D002352:Carrier Proteins; C491464:Diap1 protein, mouse; D029968:Escherichia coli Proteins; D000080865:Formins; D011725:Pyridines; C108830:Y 27632; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D020928:Mitogen-Activated Protein Kinases; D001905:Botulinum Toxins; C508203:IpgB1 protein, Shigella flexneri; C508202:IpgB2 protein, Shigella flexneri; D020830:rac1 GTP-Binding Protein; D020741:rho GTP-Binding Proteins