title: Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography.
abstract: A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of six phenolic compounds, five anthraquinones (rhein, aloe-emodin, emodin, chrysophanol and physcion) and a flavonoid (kaempferol), in root extracts from Cassia alata L. Solid-phase extraction, using C(18) cartridges, was used to remove interfering substances from the root extracts. The extracts were analyzed on a C(18) column using an isocratic mobile phase which consisted of acetonitrile, methanol, and 10mM aqueous ammonium acetate (25:55:20, v/v). Identification of the analytes was performed by use of standards and on-line mass spectrometric detection using atmospheric pressure chemical ionization. The concentration of the phenolic compounds in the root extracts was determined using HPLC with ultraviolet detection at 260nm. The limits of detection obtained for the anlytes were in the range of 0.23-4.61ppm. The overall R.S.D. precision values (intra- and inter-day) for the retention times and peak-areas were lower than 0.16 and 2.10%, respectively. In addition, the recovery of the developed method for the analysis of these phenolic compounds was determined, and ranged from 81.2+/-4.3 to 106+/-2%.
authors: Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
authors: Vivian E
authors: VE
authors: Fernand
authors: David T
authors: DT
authors: Dinh
authors: Samuel J
authors: SJ
authors: Washington
authors: Sayo O
authors: SO
authors: Fakayode
authors: Jack N
authors: JN
authors: Losso
authors: Rick O
authors: RO
authors: van Ravenswaay
authors: Isiah M
authors: IM
authors: Warner
mesh_terms: D002138:Calibration; D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts