An oxidized tryptophan facilitates copper binding in Methylococcus capsulatus-secreted protein MopE.
Proteins can coordinate metal ions with endogenous nitrogen and oxygen ligands through backbone amino and carbonyl groups, but the amino acid side chains coordinating metals do not include tryptophan. Here we show for the first time the involvement of the tryptophan metabolite kynurenine in a protein metal-binding site. The crystal structure to 1.35 angstroms of MopE* from the methane-oxidizing Methylococcus capsulatus (Bath) provided detailed information about its structure and mononuclear copper-binding site. MopE* contains a novel protein fold of which only one-third of the structure displays similarities to other known folds. The geometry around the copper ion is distorted tetrahedral with one oxygen ligand from a water molecule, two histidine imidazoles (His-132 and His-203), and at the fourth distorted tetrahedral position, the N1 atom of the kynurenine, an oxidation product of Trp-130. Trp-130 was not oxidized to kynurenine in MopE* heterologously expressed in Escherichia coli, nor did this protein bind copper. Our findings indicate that the modification of tryptophan to kynurenine and its involvement in copper binding is an innate property of M. capsulatus MopE*.
Helland
Ronny
R
Norwegian Structural Biology Centre, Faculty of Science, University of Tromso, N-9073 Tromso, Norway.
Fjellbirkeland
Anne
A
Karlsen
Odd Andre
OA
Ve
Thomas
T
Lillehaug
Johan R
JR
Jensen
Harald B
HB
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003001:Cloning, Molecular; D003300:Copper / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D007737:Kynurenine / Q000737:chemistry; D008024:Ligands; D020618:Methylococcus / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D010100:Oxygen / Q000737:chemistry*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014364:Tryptophan / Q000737:chemistry*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D008024:Ligands; D007737:Kynurenine; D003300:Copper; D014364:Tryptophan; D010100:Oxygen