Concentration changes to counteract the effects of bacteriological sampling on PLT yields.
BACKGROUND
Bacterial culturing of apheresis platelet (PLT) units appeared to increase the incidence of low yield products of <3.0 x 10(11) PLT (LYP) and decrease the incidence of multiple PLT products. To determine the effects of sampling and subsequent modifications in collections on PLT yield and adverse reactions, a retrospective analysis was performed.


METHODS
Four time periods were examined: baseline, after sampling implementation, after concentration change (Conc. Delta) implementation, and after concentration and yield target change (Target + Conc. Delta) implementation. Collections were performed on the Gambro Trima Accel. PLT concentration settings were changed from 1,550 to 1,200 (single and double PLT products) or 1,610 to 1,500 (triple PLT products). A 3.2 x 10(11) target was eliminated and a 9.4 x 10(11) target added.


RESULTS
Donors in all groups were comparable. Average incidence of LYP per week was significantly higher for Sampling (8.1%) than Baseline (2.0%) and declined significantly in the Conc. Delta (3.4%) and Target + Conc. Delta (2.0%) groups. Average PLT products/donor per week for Sampling (1.7) was significantly lower than Baseline (1.8), equivalent to Conc. Delta (1.7), and significantly lower than Target + Conc. Delta (1.9). Average weekly incidence of triple PLT products was significantly higher for Target + Conc. Delta (17%) compared with Baseline (12.4%), Sampling (10.7%), and Conc. Delta (10.8%). Donor reaction incidence was not significantly different among the groups. There was no change in recipient reactions.


CONCLUSIONS
The use of a yield target change and PLT concentration reduction compensated for changes associated with bacterial culturing without increasing donor or recipient reactions.
Burgstaler
Edwin A
EA
Division of Transfusion Medicine, Mayo Clinic, Rochester MN 55905, USA.
Mako
Amy M
AM
Winters
Jeffrey L
JL
D001782:Blood Donors; D001792:Blood Platelets / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D017713:Platelet Transfusion / Q000009:adverse effects / Q000379:methods; D010983:Plateletpheresis; D012189:Retrospective Studies