title: Coimmunoprecipitation and proteomic analyses.
abstract: Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.
authors: Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria.
authors: S Fabio
authors: SF
authors: Falsone
authors: Bernd
authors: B
authors: Gesslbauer
authors: Andreas J
authors: AJ
authors: Kungl
mesh_terms: D015153:Blotting, Western; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D036103:Nanotechnology; D040901:Proteomics