[A study on the effect of the chitosan thermosensitive hydrogel loading recombinant human bone morphogenetic protein-2 on repairing periodontal defects].
OBJECTIVE
To observe the effect of the chitosan thermosensitive hydrogel loading recombinant human bone morphogenetic protein-2 (rhBMP-2) on repairing periodontal defects.


METHODS
To prepare artificial furcation defects model in the posterior area in 3 healthy male dogs, and then to inject chitosan thermosensitive hydrogel loading of rhBMP-2 after fast suturing tissue flap. The groups filled with nothing or filled only with chitosan thermosensitive hydrogel were the controls. The dogs were sacrificed after 5 weeks and the periodontal regeneration was observed histologically.


RESULTS
The histological observation showed that the chitosan thermosensitive hydrogel loading rhBMP-2 group achieved apparent periodontal tissue regeneration occupying the majority of the defects and the control groups got only a small amount of periodontal tissue regeneration.


CONCLUSIONS
The chitosan thermosensitive hydrogel loading rhBMP-2 can effectively promote the periodontal tissue regeneration, while simplifying the surgical operation. It might be a potential means for periodontal regeneration.
Ma
Zhi-wei
ZW
Dept. of Periodontology and Mucosal Diseases, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Zhang
Yong-jie
YJ
Wu
Zhi-fen
ZF
Wang
Rong
R
Zhu
Hao
H
Li
Yuan
Y
Xu
Jie
J
Liu
Qing
Q
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D048271:Chitosan; D004285:Dogs; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008297:Male; D011994:Recombinant Proteins; D012038:Regeneration; D016212:Transforming Growth Factor beta
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan