title: A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities.
abstract: This paper presents a new polymer lab-on-a-chip for magnetic bead-based immunoassay with fully on-chip sampling and detection capabilities, which provides a smart platform of magnetic immunoassay-based lab-on-a-chip for point-of-care testing (POCT) toward biochemical hazardous agent detection, food inspection or clinical diagnostics. In this new approach, the polymer lab-on-a-chip for magnetic bead-based immunoassay consists of a magnetic bead-based separator, an interdigitated array (IDA) micro electrode, and a microfluidic system, which are fully incorporated into a lab-on-a-chip on cyclic olefin copolymer (COC). Since the polymer lab-on-a-chip was realized using low cost, high throughput polymer microfabrication techniques such as micro injection molding and hot embossing method, a disposable polymer lab-on-a-chip for the magnetic bead-based immunoassay can be successfully realized in a disposable platform. With this newly developed polymer lab-on-a-chip, an enzyme-labelled electrochemical immunoassay (ECIA) was performed using magnetic beads as the mobile solid support, and the final enzyme product produced from the ECIA was measured using chronoamperometry. A sampling and detection of as low as 16.4 ng mL(-1) of mouse IgG has been successfully performed in 35 min for the entire procedure.
authors: Microsystems and BioMEMS Laboratory, Center for BioMEMS and Nanobiosciences, Department of Electrical and Computer Engineering and Computer Science, University of Cincinnati, Cincinnati, OH 45221, USA. dohj@ececs.uc.edu
authors: Jaephil
authors: J
authors: Do
authors: Chong H
authors: CH
authors: Ahn
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis* / Q000378:metabolism; D056656:Lab-On-A-Chip Devices; D008280:Magnetics; D051379:Mice; D046208:Microchip Analytical Procedures / Q000191:economics / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D011108:Polymers