title: Comparative proteomic analysis of indioside D-triggered cell death in HeLa cells.
abstract: Medicinal plants represent a rich source of cancer drug leads. Indioside D, a furostanol glycoside isolated from Solanum mammosum, was found to possess antiproliferative activity toward a panel of human cancer cell lines. Proteomic analysis of indioside D-treated HeLa cells revealed profound protein changes related to energy production and oxidative stress, suggesting that mitochondria dysfunction plays a role in indioside D-induced apoptosis. Indioside D caused a rapid dissipation of mitochondrial transmembrane potential (DeltaPsim) and the generation of reactive oxygen species (ROS), leading to the activation of caspase-dependent apoptotic cell death. The Fas death receptor pathway was also activated following indioside D treatment, and triggered the activation of caspase-8 and cleavage of Bid, which also acted through the mitochondrial apoptosis pathway. These results suggest that indioside D induced apoptosis in HeLa cells via both intrinsic and extrinsic cell death pathways.
authors: School of Biological Sciences, Department of Anatomy, The University of Hong Kong, Hong Kong SAR, China.
authors: Chi Chun
authors: CC
authors: Wong
authors: Ying
authors: Y
authors: Wang
authors: Ka-Wing
authors: KW
authors: Cheng
authors: Jen-Fu
authors: JF
authors: Chiu
authors: Qing-Yu
authors: QY
authors: He
authors: Feng
authors: F
authors: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D020543:Proteome / Q000032:analysis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D053218:Receptors, Death Domain / Q000378:metabolism; D013256:Steroids / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D006027:Glycosides; D020543:Proteome; D017382:Reactive Oxygen Species; D053218:Receptors, Death Domain; D013256:Steroids; C428264:indioside D; D020169:Caspases