Comparison of regrowth of Enterococcus faecalis in dentinal tubules after sealing with gutta-percha or Resilon.
The objective of this study was to compare the sealing ability of gutta-percha (GP) and Resilon on Enterococcus faecalis in the dentinal tubules. Bovine root dentin blocks were infected and filled with GP alone, GP with zinc oxide-eugenol sealer, GP with resin sealer, or Resilon with resin sealer. The blocks were incubated in Brain-Heart Infusion agar plates up to 4 weeks, after which dentin chips were collected from the canal walls. The optical density of the culture medium containing the dentin chips was measured and analyzed statistically. Our results indicated that none of the filling materials were able to prevent the regrowth of E. faecalis completely. Although we noted no significant differences in bacterial counts among those groups filled with sealers, different patterns of change in bacterial counts were noted regarding time points. Filling with Resilon and a resin-based sealer induced a significant reduction in the bacterial counts over time (P < .05).
Shin
Su-Jung
SJ
Department of Conservative Dentistry, Department of Oral Biology and Oral Science Research Center, College of Dentistry, Yonsei University, Seoul, South Korea.
Jee
Sang-Wook
SW
Song
Jin-Seon
JS
Jung
Il-Young
IY
Cha
Jeong-Heon
JH
Kim
Euiseong
E
D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D003804:Dentin / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000254:growth & development; D006171:Gutta-Percha; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation / Q000379:methods*
D012387:Root Canal Filling Materials; C486892:resilon sealer; D006171:Gutta-Percha