Intragastric intubation of alcohol during the perinatal period.
Animal models of fetal alcohol spectrum disorder (FASD) have been instrumental in isolating alcohol as a teratogen and demonstrating behavioral and neural effects. There are a number of different models for rodents with various strengths and weaknesses. A three-trimester model of FASD is described here; the model uses intragastric intubation of both pregnant dams and pups to mimic alcohol exposure across all three trimesters in humans. The model does not use expensive equipment and is relatively easy to accomplish. The model allows excellent control of alcohol dose and uses an oral route of administration. There are no undernutrition effects with the doses used here. A drawback of the model is the stress of the intubation procedures and ways in which to minimize this stress are discussed. In addition, a method to measure blood alcohol levels is described.
Kelly
Sandra J
SJ
Department of Psychology, University of South Carolina, Columbia, SC, USA.
Lawrence
Charles R
CR
D000822:Animal Husbandry; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D001921:Brain / Q000187:drug effects / Q000254:growth & development*; D002492:Central Nervous System Depressants / Q000008:administration & dosage* / Q000097:blood / Q000633:toxicity; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood / Q000633:toxicity; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D007263:Infusions, Parenteral; D007441:Intubation, Gastrointestinal; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D020318:Rats, Long-Evans
D002492:Central Nervous System Depressants; D000431:Ethanol