Promoter methylation analysis of SIRT3, SMARCA5, HTERT and CDH1 genes in aging and Alzheimer's disease.
Longevity related genes were investigated concerning promoter methylation. SIRT3, SMARCA5, HTERT and CDH1 promoters were analyzed in peripheral blood in relation to gender, age and Alzheimer's disease (AD). Methylation Specific PCR assay (MSP) was used. There were no significant differences in methylation frequencies of SIRT3, SMARCA5 and CDH1 among young, elderly and AD groups (p> 0.05), showing no association with aging or AD. On the other hand, HTERT methylation frequency was associated with the aging process, in that AD patients differed from elderly controls (p=0.0086), probably due to telomere and immune dysfunctions involved in AD pathogenesis.
Silva
Patrícia Natália Oliveira
PN
Department of Morphology, Genetics Division, Universidade Federal de São Paulo (UNIFESP/EPM), São Paulo, SP, Brazil.
Gigek
Carolina Oliveira
CO
Leal
Mariana Ferreira
MF
Bertolucci
Paulo Henrique Ferreira
PH
de Labio
Roger Willian
RW
Payão
Spencer Luiz Marques
SL
Smith
Marília de Arruda Cardoso
Mde A
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000368:Aged; D000375:Aging / Q000502:physiology*; D000544:Alzheimer Disease / Q000235:genetics*; D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D019175:DNA Methylation; D006801:Humans; D024101:Mitochondrial Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D056566:Sirtuin 3; D037761:Sirtuins / Q000235:genetics*; D019098:Telomerase / Q000235:genetics*
D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D002868:Chromosomal Proteins, Non-Histone; D024101:Mitochondrial Proteins; C509186:TERT protein, human; D019098:Telomerase; C466093:SIRT3 protein, human; D056566:Sirtuin 3; D037761:Sirtuins; D000251:Adenosine Triphosphatases; C409462:SMARCA5 protein, human