title: The lifestyle of Corynebacterium urealyticum derived from its complete genome sequence established by pyrosequencing.
abstract: Corynebacterium urealyticum is a lipid-requiring, urealytic bacterium of the human skin flora that has been recognized as causative agent of urinary tract infections. We report the analysis of the complete genome sequence of C. urealyticum DSM7109, which was initially recovered from a patient with alkaline-encrusted cystitis. The genome sequence was determined by a combination of pyrosequencing and Sanger technology. The chromosome of C. urealyticum DSM7109 has a size of 2,369,219bp and contains 2024 predicted coding sequences, of which 78% were considered as orthologous with genes in the Corynebacterium jeikeium K411 genome. Metabolic analysis of the lipid-requiring phenotype revealed the absence of a fatty acid synthase gene and the presence of a beta-oxidation pathway along with a large repertoire of auxillary genes for the degradation of exogenous fatty acids. A urease locus with the gene order ureABCEFGD may play a pivotal role in virulence of C. urealyticum by the alkalinization of human urine and the formation of struvite stones. Multidrug resistance of C. urealyticum DSM7109 is mediated by transposable elements, conferring resistances to macrolides, lincosamides, ketolides, aminoglycosides, chloramphenicol, and tetracycline. The complete genome sequence of C. urealyticum revealed a detailed picture of the lifestyle of this opportunistic human pathogen.
authors: Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universität Bielefeld, Universitätsstrasse 25, D-33615 Bielefeld, Germany. Andreas.Tauch@Genetik.Uni-Bielefeld.DE
authors: Andreas
authors: A
authors: Tauch
authors: Eva
authors: E
authors: Trost
authors: Alexandra
authors: A
authors: Tilker
authors: Ulrike
authors: U
authors: Ludewig
authors: Susanne
authors: S
authors: Schneiker
authors: Alexander
authors: A
authors: Goesmann
authors: Walter
authors: W
authors: Arnold
authors: Thomas
authors: T
authors: Bekel
authors: Karina
authors: K
authors: Brinkrolf
authors: Iris
authors: I
authors: Brune
authors: Susanne
authors: S
authors: Götker
authors: Jörn
authors: J
authors: Kalinowski
authors: Paul-Bertram
authors: PB
authors: Kamp
authors: Francisco Pereira
authors: FP
authors: Lobo
authors: Prisca
authors: P
authors: Viehoever
authors: Bernd
authors: B
authors: Weisshaar
authors: Francisco
authors: F
authors: Soriano
authors: Marcus
authors: M
authors: Dröge
authors: Alfred
authors: A
authors: Pühler
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003352:Corynebacterium / Q000235:genetics*; D016680:Genome, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D001426:Bacterial Proteins