title: Infectivity studies of influenza virus hemagglutinin receptor binding site mutants in mice.
abstract: The replicative properties of influenza virus hemagglutinin (HA) mutants with altered receptor binding characteristics were analyzed following intranasal inoculation of mice. Among the mutants examined was a virus containing a Y98F substitution at a conserved position in the receptor binding site that leads to a 20-fold reduction in binding. This mutant can replicate as well as wild-type (WT) virus in MDCK cells and in embryonated chicken eggs but is highly attenuated in mice, exhibiting titers in lungs more than 1,000-fold lower than those of the WT. The capacity of the Y98F mutant to induce antibody responses and the structural locations of HA reversion mutations are examined.
authors: Emory University School of Medicine, Atlanta, GA 30322, USA.
authors: Jeffrey
authors: J
authors: Meisner
authors: Kristy J
authors: KJ
authors: Szretter
authors: Konrad C
authors: KC
authors: Bradley
authors: William A
authors: WA
authors: Langley
authors: Zhu-Nan
authors: ZN
authors: Li
authors: Byeong-Jae
authors: BJ
authors: Lee
authors: Sudha
authors: S
authors: Thoennes
authors: Javier
authors: J
authors: Martin
authors: John J
authors: JJ
authors: Skehel
authors: Rupert J
authors: RJ
authors: Russell
authors: Jacqueline M
authors: JM
authors: Katz
authors: David A
authors: DA
authors: Steinhauer
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001665:Binding Sites; D001835:Body Weight; D002460:Cell Line; D002642:Chick Embryo; D004285:Dogs; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000378:metabolism*; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D020125:Mutation, Missense; D009976:Orthomyxoviridae Infections / Q000517:prevention & control / Q000821:virology; D017434:Protein Structure, Tertiary; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014774:Virulence; D014779:Virus Replication / Q000502:physiology
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014764:Viral Proteins; C458881:hemagglutinin fusogenic peptide, influenza virus