Insights into the role of bone marrow-derived stem cells in renal repair.
Acute kidney injury (AKI) is a frequent clinical problem with a high mortality rate, generally caused by ischemic insults. Nevertheless, the kidney has a remarkably high capacity to regenerate after ischemic injury. Tubular cells can restore renal function by proliferation and dedifferentiation into a mesenchymal cell type, but also stem cells residing in bone marrow may contribute. We compiled a protocol from several published methods to study the contribution of bone marrow-derived cells to renal regeneration. Bone marrow was isolated from donor FVB mice and labeled with enhanced green fluorescent protein (eGFP) through adenovirus transduction. After cell sorting, eGFP-labeled cells were transplanted in sublethally irradiated recipient FVB mice. Four weeks after transplantation, we provoked AKI in mice by inducing unilateral ischemic-reperfusion injury for 30 min. Seven days after the injury, eGFP-positive bone marrow-derived cells were clearly detectable in ischemic kidney tissue, and they contribute to the regeneration of approximately 10% of proximal tubular mass. In this review the advantages and shortcomings of our procedure are critically discussed and compared with other methods described.
Huls
Miriam
M
Department of Pharmacology and Toxicology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands.
Russel
Frans G M
FG
Masereeuw
Rosalinde
R
D058186:Acute Kidney Injury / Q000628:therapy*; D000818:Animals; D016026:Bone Marrow Transplantation / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D004195:Disease Models, Animal; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D012038:Regeneration / Q000502:physiology; D015427:Reperfusion Injury