In vitro and in vivo effectiveness of egg yolk antibody against Candida albicans (anti-CA IgY).
We prepared anti-Candida albicans antibody in chicken egg yolk (anti-CA IgY) and investigated its in vitro and in vivo effectiveness. Anti-CA IgY significantly reduced the adherence capacity of C. albicans to FaDu cells (human pharynx carcinoma cells) in a dose-dependent manner. The protective efficacy of anti-CA IgY was investigated in experimentally induced oral candidiasis in immunosuppressed mice. Oral administration of anti-CA IgY significantly reduced the number of C. albicans and the scores of the tongue lesions. Moreover, anti-CA IgY reduced the colonization of C. albicans in mice organs. These results indicate that anti-CA IgY has a protective effect against the oral candidiasis of experimentally infected mice and reduces the dissemination of C. albicans. Putting together, these results indicate that anti-CA IgY is effective against C. albicans. This effect might be due to the blocking of the binding of C. albicans to the host cells. Therefore, anti-CA IgY might be considered as a prophylactic immunotherapy or possibly an adjunctive antifungal therapy.
Ibrahim
El-Sayed Moustafa
el-SM
Immunology Research Institute, GHEN Corporation, 839-1 Sano, Gifu 501-1101, Japan. ibrahim@ghen.co.jp
Rahman
A K M Shofiqur
AK
Isoda
Rie
R
Umeda
Kouji
K
Van Sa
Nguyen
N
Kodama
Yoshikatsu
Y
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000276:immunology; D002177:Candidiasis / Q000188:drug therapy / Q000276:immunology* / Q000517:prevention & control; D002180:Candidiasis, Oral / Q000382:microbiology; D045744:Cell Line, Tumor; D004530:Egg Yolk / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D051379:Mice
D000908:Antibodies, Fungal; C016512:IgY; D007136:Immunoglobulins