[Use of multiplex PCR for the diagnosis of Theileria annulata and Theileria buffeli].
The species causing theileriosis in cattle in Turkey are Theileria annulata and T. buffeli. While T. buffeli is low in pathogenicity or non-pathogenic , T. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. In this study, a multiplex PCR was used for a simultaneous diagnosis of these species. Genes for the merozoite surface antigen (Tams 1) and the major piroplasm surface protein (MPSP) were amplified with PCR for T. annulata and T. buffeli, respectively. It was found that both single and mixed infection with T. annulata and T. buffeli could be diagnosed with multiplex PCR.
Altay
Kürşat
K
Firat Universitesi Veteriner Fakültesi Parazitoloji Anabilim Dali, Elaziğ, Turkey. kaltay@firat.edu.tr
Aydin
Mehmet Fatih
MF
Uluişik
Uğur
U
Aktaş
Münir
M
Dumanli
Nazir
N
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002417:Cattle; D016054:DNA, Protozoan / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D015800:Protozoan Proteins / Q000235:genetics; D016794:Theileria / Q000235:genetics / Q000302:isolation & purification*; D016796:Theileria annulata / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D013801:Theileriasis / Q000175:diagnosis* / Q000469:parasitology
D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C113538:Tams1 antigen; C115173:major piroplasm surface protein, Theileria