Evaluation of a commercial direct fluorescent-antibody assay for human metapneumovirus in respiratory specimens.
The performance of a commercially manufactured direct fluorescent-antibody assay kit for human metapneumovirus was compared to that of reverse transcriptase PCR, the current "gold standard." The kit demonstrated a sensitivity of 95.2%, a specificity of 100%, and an accuracy of 98.9%.
Vinh
Donald C
DC
Department of Medical Microbiology, Clinical Virology Laboratory, McGill University Health Centre, Montreal, Quebec, Canada.
Newby
Diane
D
Charest
Hugues
H
McDonald
Jane
J
D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D029121:Metapneumovirus / Q000276:immunology / Q000302:isolation & purification*; D008875:Middle Aged; D018184:Paramyxoviridae Infections / Q000175:diagnosis*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity