title: Trypanosoma brucei mitochondrial ribosomes: affinity purification and component identification by mass spectrometry.
abstract: Although eukaryotic mitochondrial (mt) ribosomes evolved from a putative prokaryotic ancestor their compositions vary considerably among organisms. We determined the protein composition of tandem affinity-purified Trypanosoma brucei mt ribosomes by mass spectrometry and identified 133 proteins of which 77 were associated with the large subunit and 56 were associated with the small subunit. Comparisons with bacterial and mammalian mt ribosomal proteins identified T. brucei mt homologs of L2-4, L7/12, L9, L11, L13-17, L20-24, L27-30, L33, L38, L43, L46, L47, L49, L52, S5, S6, S8, S9, S11, S15-18, S29, and S34, although the degree of conservation varied widely. Sequence characteristics of some of the component proteins indicated apparent functions in rRNA modification and processing, protein assembly, and mitochondrial metabolism implying possible additional roles for these proteins. Nevertheless most of the identified proteins have no homology outside Kinetoplastida implying very low conservation and/or a divergent function in kinetoplastid mitochondria.
authors: Seattle Biomedical Research Institute, Seattle, Washington 98109, USA.
authors: Alena
authors: A
authors: Zíková
authors: Aswini K
authors: AK
authors: Panigrahi
authors: Rachel A
authors: RA
authors: Dalley
authors: Nathalie
authors: N
authors: Acestor
authors: Atashi
authors: A
authors: Anupama
authors: Yuko
authors: Y
authors: Ogata
authors: Peter J
authors: PJ
authors: Myler
authors: Kenneth
authors: K
authors: Stuart
mesh_terms: D000465:Algorithms; D000818:Animals; D030801:Animals, Genetically Modified; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry; D054657:Ribosome Subunits / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D014346:Trypanosoma brucei brucei / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D012269:Ribosomal Proteins