title: Diversity in coffee assessed with SSR markers: structure of the genus Coffea and perspectives for breeding.
abstract: The present study shows transferability of microsatellite markers developed in the two cultivated coffee species (Coffea arabica L. and C. canephora Pierre ex Froehn.) to 15 species representing the previously identified main groups of the genus Coffea. Evaluation of the genetic diversity and available resources within Coffea and development of molecular markers transferable across species are important steps for breeding of the two cultivated species. We worked on 15 species with 60 microsatellite markers developed using different strategies (SSR-enriched libraries, BAC libraries, gene sequences). We focused our analysis on 4 species used for commercial or breeding purposes. Our results establish the high transferability of microsatellite markers within Coffea. We show the large amount of diversity available within wild species for breeding applications. Finally we discuss the consequences for future comparative mapping studies and breeding of the two cultivated species.
authors: CIRAD, UMR DAP, TA A-96/03, avenue Agropolis, 34398 Montpellier Cedex 5, France. philippe.cubry@cirad.fr
authors: Philippe
authors: P
authors: Cubry
authors: Pascal
authors: P
authors: Musoli
authors: Hyacinte
authors: H
authors: Legnaté
authors: David
authors: D
authors: Pot
authors: Fabien
authors: F
authors: de Bellis
authors: Valérie
authors: V
authors: Poncet
authors: François
authors: F
authors: Anthony
authors: Magali
authors: M
authors: Dufour
authors: Thierry
authors: T
authors: Leroy
mesh_terms: D001947:Breeding; D040503:Coffea / Q000145:classification / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats
chemical_list: D005819:Genetic Markers