Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase antibodies for universal detection.
The biosynthesis of steroids from steroidogenic cells are catalyzed by the two major enzymes, P450 side-chain cleavage enzyme (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD). This article describes the construction of two novel polyclonal antibodies against conserved recombinant protein and the validation of these antibodies on fixed tissue sections of bovine corpus luteum. The polyclonal antibodies were used successfully in Western blots and specifically reacted with P450scc and 3beta-HSD protein in bovine luteal cell extracts. Thus, P450scc and 3beta-HSD are two specific polyclonal antibodies that are integral products in the investigation of the biological function and regulatory mechanism involved in steroidogenesis.
Chiu
Chih-Hsien
CH
Laboratory of Animal Physiology, Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan, ROC.
Wei
Hen-Wei
HW
Wu
Leang-Shin
LS
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D002417:Cattle; D045424:Complex Mixtures / Q000737:chemistry / Q000276:immunology; D003338:Corpus Luteum / Q000737:chemistry / Q000201:enzymology / Q000276:immunology*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000276:immunology*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D045424:Complex Mixtures; D012739:Gonadal Steroid Hormones; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D015096:3-Hydroxysteroid Dehydrogenases