Optimum diagnostic assay and clinical specimen for routine rotavirus surveillance.
Rates of detection of rotavirus were compared by diagnostic assay and specimen type. For bulk stools, rates of detection by reverse transcriptase PCR (RT-PCR) and enzyme immunoassay (EIA) were similar, but 18% of healthy controls tested positive by RT-PCR. Testing of bulk stools by EIA appears to be optimum for rotavirus surveillance.
Stockman
Lauren J
LJ
National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. Lstockman@cdc.gov
Staat
Mary A
MA
Holloway
Michol
M
Bernstein
David I
DI
Kerin
Tara
T
Hull
Jennifer
J
Yee
Eileen
E
Gentsch
Jon
J
Parashar
Umesh D
UD
D002675:Child, Preschool; D005243:Feces / Q000821:virology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D008403:Mass Screening / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*