Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays.
Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.
Ellmark
P
P
Department of Immunotechnology, Lund University, BMC D13, SE-221 84 Lund, Sweden. peter.ellmark@immun.lth.se
Högerkorp
C M
CM
Ek
S
S
Belov
L
L
Berglund
M
M
Rosenquist
R
R
Christopherson
R I
RI
Borrebaeck
C A K
CA
D000293:Adolescent; D000906:Antibodies; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D025341:Principal Component Analysis; D040081:Protein Array Analysis; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*
D000906:Antibodies; D015703:Antigens, CD; D011947:Receptors, Antigen, B-Cell