title: Nanoporous niobium oxide for label-free detection of DNA hybridization events.
abstract: We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1nM.
authors: Nanomaterials Application Division, Korea Institute of Ceramic Engineering and Technology (KICET), Seoul 153-801, South Korea. jinsub@kicet.re.kr
authors: Jinsub
authors: J
authors: Choi
authors: Jae Hoon
authors: JH
authors: Lim
authors: Sangchul
authors: S
authors: Rho
authors: Deokjin
authors: D
authors: Jahng
authors: Jaeyoung
authors: J
authors: Lee
authors: Kyung Ja
authors: KJ
authors: Kim
mesh_terms: D004247:DNA / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D009556:Niobium / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared
chemical_list: D009556:Niobium; D004247:DNA