Analysis of the C. elegans germline stem cell region.
We present methods for characterizing the mitotic and early meiotic regions of the Caenorhabditis elegans germline. The methods include examination of germlines in living and fixed worms, cell cycle analysis, analysis of markers, and initial characterization of mutants that affect germline proliferation.
Crittenden
Sarah L
SL
Howard Hughes Medical Institute and Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA.
Kimble
Judith
J
D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D002453:Cell Cycle; D049109:Cell Proliferation; D005260:Female; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D008297:Male; D008540:Meiosis; D008857:Microscopy, Interference; D008938:Mitosis; D008940:Mitotic Index; D008967:Molecular Biology / Q000379:methods; D009154:Mutation
D015415:Biomarkers; D001973:Bromodeoxyuridine