[Effect of epigallocatechin-3-gallate on the invasiveness of hepatocarcinoma cells in vitro].
OBJECTIVE
The effect of EGCG on the invasion of HepG2 cells in vitro was studied.


METHODS
The expressions of MUC1 in HepG2 cells before and after they were treated with EGCG were studied with immunohistochemistry. The effects of EGCG on the invasion of HepG2 cells were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membranes (Matrigel). Gelatin zymography was performed to detect the secretion of MMP-2 and MMP-9.


RESULTS
EGCG reduced the expression of MUC1, suppressed significantly the invasion of tumor cells into the basement membranes (P < 0.01) and reduced the secretion of MMP-2, MMP-9.


CONCLUSIONS
EGCG has anti-invasion effects on HepG2 cells.
Lu
Li
L
Hangzhou No. 6 Hospital, China. luli7711@163.com
Liu
Hui-Min
HM
Tang
Wei-Xue
WX
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion; D002470:Cell Survival; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D018396:Mucin-1 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010936:Plant Extracts / Q000494:pharmacology*
C060192:MUC1 protein, human; D018396:Mucin-1; D010936:Plant Extracts; D002392:Catechin; C045651:epigallocatechin gallate; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9