A novel function of bamboo extract in relieving lipotoxicity.
Lipotoxicity is closely related to the etiology and complications of type 2 diabetes mellitus. This study investigated the protective effect of an extract from bamboo Phyllostachys edulis against palmitic acid (PA)-induced lipoapoptosis. The lipo-detoxification function of the bamboo extract (BEX) was evaluated using cell culture models. Cell viability was measured by MTT assay and cell apoptosis was monitored by Annexin V staining. Cellular uptake of fluorescent free fatty acid (FFA) analog was measured by flow cytometry. Protein levels of total protein kinase B (Akt) and phosphorylated Akt (p-Akt) were measured by western blotting. The results show that co-incubating BEX with mouse myoblast C2C12 cells had no effect on the cellular uptake of FFA, but dramatically decreased PA-induced cell apoptosis and protected cell viability. A similar antilipotoxicity effect of BEX was observed in other mammalian cells. BEX significantly decreased the protein levels of both Akt and p-Akt in C2C12 cells under normal cell culture conditions but not under lipotoxic conditions, indicating the regulatory effect of BEX on cell signaling pathways and its response to a high FFA environment. This study demonstrated a novel function of bamboo extract in preventing lipotoxicity in mammalian cells, implicating a promising phytotherapeutic approach for lipo-detoxification.
Panee
Jun
J
Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii at Manoa, 651 Ilalo Street BSB 222, Honolulu, HI 96813, USA. junchen@hawaii.edu
Liu
Wanyu
W
Lin
Yanling
Y
Gilman
Christy
C
Berry
Marla J
MJ
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D031723:Bambusa / Q000737:chemistry*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005434:Flow Cytometry; D051379:Mice; D019308:Palmitic Acid / Q000633:toxicity*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D014709:Vero Cells
D005230:Fatty Acids, Nonesterified; D010936:Plant Extracts; D019308:Palmitic Acid; D051057:Proto-Oncogene Proteins c-akt