Activity-based protein profiling: from enzyme chemistry to proteomic chemistry.
Genome sequencing projects have provided researchers with a complete inventory of the predicted proteins produced by eukaryotic and prokaryotic organisms. Assignment of functions to these proteins represents one of the principal challenges for the field of proteomics. Activity-based protein profiling (ABPP) has emerged as a powerful chemical proteomic strategy to characterize enzyme function directly in native biological systems on a global scale. Here, we review the basic technology of ABPP, the enzyme classes addressable by this method, and the biological discoveries attributable to its application.
Cravatt
Benjamin F
BF
The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA. cravatt@scripps.edu
Wright
Aaron T
AT
Kozarich
John W
JW
D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D003546:Cysteine Endopeptidases / Q000737:chemistry; D016678:Genome; D006801:Humans; D006867:Hydrolases / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D040901:Proteomics / Q000379:methods*
D011506:Proteins; D020543:Proteome; D000255:Adenosine Triphosphate; D006867:Hydrolases; D003546:Cysteine Endopeptidases