title: Transparent adult zebrafish as a tool for in vivo transplantation analysis.
abstract: The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.
authors: Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA.
authors: Richard Mark
authors: RM
authors: White
authors: Anna
authors: A
authors: Sessa
authors: Christopher
authors: C
authors: Burke
authors: Teresa
authors: T
authors: Bowman
authors: Jocelyn
authors: J
authors: LeBlanc
authors: Craig
authors: C
authors: Ceol
authors: Caitlin
authors: C
authors: Bourque
authors: Michael
authors: M
authors: Dovey
authors: Wolfram
authors: W
authors: Goessling
authors: Caroline Erter
authors: CE
authors: Burns
authors: Leonard I
authors: LI
authors: Zon
mesh_terms: D000417:Albinism / Q000235:genetics; D000818:Animals; D005434:Flow Cytometry; D008027:Light; D023421:Models, Animal; D009368:Neoplasm Transplantation; D010858:Pigmentation / Q000235:genetics*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000648:ultrastructure*; D015027:Zebrafish