title: Neuronal maturation in an experimental model of brain tissue heterotopia in the lung.
abstract: Neural maturation involves diverse interaction and signaling mechanisms that are essential to the development of the nervous system. However, little is known about the development of neurons in heterotopic brain tissue in the lung, a rare abnormality observed in malformed babies and fetuses. The aim of this study was to identify the neurons and to investigate their maturation in experimental brain tissue heterotopia during fetal and neonatal periods. The fetuses from 24 pregnant female Swiss mice were used to induce brain tissue heterotopia on the 15th gestational day. Briefly, the brain of one fetus of each dam was extracted, disaggregated, and injected into the right hemithorax of siblings. Six of these fetuses with pulmonary brain tissue implantation were collected on the 18th gestational day (group E18), and six others were collected on the 8th postnatal day (group P8). The brain of each fetus from dams not submitted to any experimental procedure was collected on the 18th gestational day (group CE18) and on the 8th postnatal day (group CP8) to serve as a control for neuronal quantitation and maturation. Immunohistochemical staining of NeuN was used to assess neuron quantity and maturation. The NeuN labeling index was greater in the postnatal period than in the fetal period for the experimental and control groups (P8 > E18 and CP8 > CE18), although there were fewer neurons in experimental than in control groups (P8 < CP8 and E18 < CE18) (P < 0.005). These results indicate that fetal neuroblasts/neurons not only survive a dramatic event such as mechanical disaggregation, in the same way as it happens in human cases, but also they retain their development in heterotopia, irrespective of local tissue influences.
authors: Department of Pathology, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto - SP -Brazil, 14049-900.
authors: Paulo Roberto Veiga
authors: PR
authors: Quemelo
authors: Luiz Cesar
authors: LC
authors: Peres
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain; D002454:Cell Differentiation; D002828:Choristoma; D004195:Disease Models, Animal; D005260:Female; D008171:Lung Diseases / Q000473:pathology*; D008297:Male; D051379:Mice; D009474:Neurons / Q000473:pathology*