The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions.
Saliva is a body fluid with important functions in oral and general health. A consortium of three research groups catalogued the proteins in human saliva collected as the ductal secretions: 1166 identifications--914 in parotid and 917 in submandibular/sublingual saliva--were made. The results showed that a high proportion of proteins that are found in plasma and/or tears are also present in saliva along with unique components. The proteins identified are involved in numerous molecular processes ranging from structural functions to enzymatic/catalytic activities. As expected, the majority mapped to the extracellular and secretory compartments. An immunoblot approach was used to validate the presence in saliva of a subset of the proteins identified by mass spectrometric approaches. These experiments focused on novel constituents and proteins for which the peptide evidence was relatively weak. Ultimately, information derived from the work reported here and related published studies can be used to translate blood-based clinical laboratory tests into a format that utilizes saliva. Additionally, a catalogue of the salivary proteome of healthy individuals allows future analyses of salivary samples from individuals with oral and systemic diseases, with the goal of identifying biomarkers with diagnostic and/or prognostic value for these conditions; another possibility is the discovery of therapeutic targets.
Denny
Paul
P
School of Dentistry, University of Southern California, Los Angeles, California 90089, USA.
Hagen
Fred K
FK
Hardt
Markus
M
Liao
Lujian
L
Yan
Weihong
W
Arellanno
Martha
M
Bassilian
Sara
S
Bedi
Gurrinder S
GS
Boontheung
Pinmannee
P
Cociorva
Daniel
D
Delahunty
Claire M
CM
Denny
Trish
T
Dunsmore
Jason
J
Faull
Kym F
KF
Gilligan
Joyce
J
Gonzalez-Begne
Mireya
M
Halgand
Frédéric
F
Hall
Steven C
SC
Han
Xuemei
X
Henson
Bradley
B
Hewel
Johannes
J
Hu
Shen
S
Jeffrey
Sherry
S
Jiang
Jiang
J
Loo
Joseph A
JA
Ogorzalek Loo
Rachel R
RR
Malamud
Daniel
D
Melvin
James E
JE
Miroshnychenko
Olga
O
Navazesh
Mahvash
M
Niles
Richard
R
Park
Sung Kyu
SK
Prakobphol
Akraporn
A
Ramachandran
Prasanna
P
Richert
Megan
M
Robinson
Sarah
S
Sondej
Melissa
M
Souda
Puneet
P
Sullivan
Mark A
MA
Takashima
Jona
J
Than
Shawn
S
Wang
Jianghua
J
Whitelegge
Julian P
JP
Witkowska
H Ewa
HE
Wolinsky
Lawrence
L
Xie
Yongming
Y
Xu
Tao
T
Yu
Weixia
W
Ytterberg
Jimmy
J
Wong
David T
DT
Yates
John R
JR
Fisher
Susan J
SJ
D000328:Adult; D001798:Blood Proteins / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000737:chemistry*; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D013361:Sublingual Gland / Q000737:chemistry*; D013363:Submandibular Gland / Q000737:chemistry*; D013666:Tears / Q000737:chemistry
D001798:Blood Proteins; D020543:Proteome; D012471:Salivary Proteins and Peptides