title: Adult neural stem cells.
abstract: Neural stem cells (NSCs) have been identified in the mature central nervous system (CNS), and they reside in specific areas. Cultures of NSCs can be successfully established in vitro by exploiting the NeuroSphere assay. This methodology relies on the continuous exposure of neural cells to mitogens such as epidermal growth factor and fibroblast growth factor-2. Under these conditions, only NSCs and highly undifferentiated progenitors proliferate, whereas committed precursors and terminally differentiated cells are eliminated from the culture. The proper application of this method to the cells allows the establishment of long-term expanding stable NSC lines, starting from different neural tissues as the adult rodent CNS and human brain tumor specimens.
authors: Stem Cell Research Institute (HSR-SCRI), DIBI-H.S. Raffaele, Milan, Italy.
authors: Rossella
authors: R
authors: Galli
authors: Angela
authors: A
authors: Gritti
authors: Angelo L
authors: AL
authors: Vescovi
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000473:pathology; D004210:Dissection; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology; D009474:Neurons / Q000166:cytology*; D012377:Rodentia; D013234:Stem Cells / Q000166:cytology*