[The effect of different concentrations of platelet-rich plasma on human periodontal ligament cells in vitro].
OBJECTIVE
To assess the effect of different concentrations of platelet-rich plasma (PRP) on cell proliferation and alkaline phosphatase (ALP) activity of human periodontal ligament cells (hPDLCs) in vitro.


METHODS
PRP was obtained from volunteer donors using two-step centrifugation. TGF-beta1 and PDGF-AB levels in activated PRP, PRP and plasma were evaluated by enzyme-linked immunosorbent assay. hPDLCs were exposed to various concentrations of PRP (2%,5%,10% and 20%) and DMEM (negative control), respectively. After 24 and 72 hours, cell proliferation was evaluated using Cell Counting Kit-8 assay. Alkaline phosphatase (ALP) activity of hPDLCs was evaluated by a p-nitrophenyl phosphate disodium assay. Statistical analysis was performed using one-way ANOVA and Student's t test in SPSS10.0.


RESULTS
TGF-beta1 and PDGF-AB levels were highest in activated PRP. The effect of various concentrations of PRP on cell proliferation and ALP activity was significantly greater than that of negative control (P<0.001). The effect of PRP was significantly greater at 72-hour compared with that at 24-hour (P<0.01), with significantly difference among various PRP concentrations (P<0.001). Cell proliferation and ALP activity increased when the concentration of PRP increased from 2% to 10%. The maximum effect on cell proliferation was achieved with 10% PRP; 20% PRP resulted in a reduced cell proliferation. However, ALP activity was greatest with 20% PRP.


CONCLUSIONS
PRP preparations exert a dose-dependent effect on hPDLCs proliferation and ALP activity in a certain extent in vitro at 24-hour and 72-hour. Supported by the Capital Medical Development Fund (Grant No. 2005-2007).
Qiao
Jing
J
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Ouyang
Xiang-Ying
XY
Cao
Cai-Fang
CF
D000469:Alkaline Phosphatase; D001792:Blood Platelets; D049109:Cell Proliferation; D006801:Humans; D066298:In Vitro Techniques; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010513:Periodontal Ligament; D010982:Platelet-Derived Growth Factor; D053657:Platelet-Rich Plasma; D053773:Transforming Growth Factor beta1
D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010982:Platelet-Derived Growth Factor; D053773:Transforming Growth Factor beta1; C095477:platelet-derived growth factor AB; C008644:nitrophenylphosphate; D000469:Alkaline Phosphatase