A multiplex TaqMan PCR assay for the detection of measles and rubella virus.
Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.
Hübschen
Judith M
JM
Institute of Immunology and WHO Collaborative Centre for Measles and WHO European Regional Reference Laboratory for Measles and Rubella, Laboratoire National de Santé, 20A rue Auguste Lumière, L-1950 Luxembourg, Luxembourg.
Kremer
Jacques R
JR
De Landtsheer
Sébastien
S
Muller
Claude P
CP
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D008457:Measles / Q000175:diagnosis*; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification*; D016415:Sequence Alignment
D017931:DNA Primers; D012367:RNA, Viral