Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Flavobacterium columnare.
Outer membrane proteins (OMPs) of bacteria are key molecules interacting with the host environment. Flavobacterium columnare, a pathogen-causing columnaris disease of fish worldwide, was studied in order to understand the composition of its OMPs. The sarcosine-insoluble membrane fraction of the OMPs was analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in combination with reverse-phase high-performance liquid chromatography-tandem mass spectrometry (RP-HPLC MS/MS). Thirty-six proteins were identified, including proteins involved in cell wall/membrane biogenesis, specific transport of various nutrients and in essential metabolism. The present study is the first report on the OMPs of F. columnare, and may serve as the basis for understanding the pathogenesis of the bacterium.
Liu
G Y
GY
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Nie
P
P
Zhang
J
J
Li
N
N
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000502:physiology; D002347:Carps / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D005417:Flavobacterium / Q000737:chemistry*; D040901:Proteomics; D012521:Sarcosine / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000662:veterinary
D001425:Bacterial Outer Membrane Proteins; D012521:Sarcosine