Nanoporous niobium oxide for label-free detection of DNA hybridization events.
We found that DNA probes can be immobilized on anodically prepared porous niobium oxide without a chemical modification of both the DNA probes and the substrate. By using the porous niobium oxide with a positive surface charge, DNA hybridization events are detected on the basis of the blue-shift of a maximum absorption peak in UV-vis-NIR spectroscopy. The blue-shift is ascribed to the change of surface charge upon single- or double-stranded DNA. The method does not require a label and shows high sensitivity with the detection limit of the concentration of 1nM.
Choi
Jinsub
J
Nanomaterials Application Division, Korea Institute of Ceramic Engineering and Technology (KICET), Seoul 153-801, South Korea. jinsub@kicet.re.kr
Lim
Jae Hoon
JH
Rho
Sangchul
S
Jahng
Deokjin
D
Lee
Jaeyoung
J
Kim
Kyung Ja
KJ
D004247:DNA / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D009556:Niobium / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared
D009556:Niobium; D004247:DNA