title: Molecular epidemiology of adenovirus type 3 detected from 1994 to 2006 in Hyogo Prefecture, Japan.
abstract: The molecular epidemiology of 126 adenovirus type 3 (AdV3) isolates obtained in Hyogo Prefecture (population: 5.5 million) from 1994 to 2006 was studied. The hexon-coding region, including 7 hypervariable regions (HVRs) (1,419 bp), was sequenced. We found 5 nonsynonymous nucleotide substitutions in the HVRs. The results are strongly suggestive of positive Darwinian selection. We classified the AdV3 strains analyzed here into 3 genome types: AdV3x (n=44), AdV3y (n=46), and AdV3z (n=36). AdV3x first appeared in 2001 in Hyogo Prefecture, and was detected predominantly during a large outbreak of AdV3 in 2003-2005. AdV3x was identical to a Korean strain responsible for a large outbreak of AdV3 in Korea in 1998-1999. We conclude that at least 3 genome types of AdV3 have circulated in Hyogo Prefecture, Japan, during the past 13 years (1994-2006). The findings also suggest that AdV3x was imported from Korea to Hyogo Prefecture in 2001.
authors: National Institute of Infectious Diseases, Tokyo 162-8640, Japan. fujimo-t@nih.go.jp
authors: Tsuguto
authors: T
authors: Fujimoto
authors: Itsuki
authors: I
authors: Hamamoto
authors: Kiyosu
authors: K
authors: Taniguchi
authors: Masatsugu
authors: M
authors: Chikahira
authors: Nobuhiko
authors: N
authors: Okabe
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology*; D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D002522:Chlorocebus aethiops; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D030561:Databases, Nucleic Acid; D016679:Genome, Viral; D006367:HeLa Cells; D006801:Humans; D007564:Japan / Q000453:epidemiology; D017720:Molecular Epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014709:Vero Cells
chemical_list: D036022:Capsid Proteins; D022801:Complementarity Determining Regions; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus