In vivo tracking of human mesenchymal stem cells in experimental stroke.
To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model of middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed, hMSCs were labeled with ferumoxides (Feridex)--protamine sulfate complexes, which were visualized and examined by MRI up to 10 weeks following transplantation. Migration of the transplanted cells to the infarcted area was further confirmed by histological methods. We found that the hMSCs transplanted in MCAo models possess the capacity to migrate to the infarcted area extensively in both ipsilateral and contralateral injections, exhibiting a pathotropism. We also analyzed the detailed migration patterns of transplanted hMSCs. We speculate that the extensive migratory ability of hMSCs may represent a therapeutic potential for developing efficient cell transplantation strategies in stroke.
Kim
Daehong
D
MRI Team, Korea Basic Science Institute, Ochang, Korea.
Chun
Byoung-Gi
BG
Kim
Yeon-Kyung
YK
Lee
Yong Hyun
YH
Park
Cheong-Soo
CS
Jeon
Iksoo
I
Cheong
Chaejoon
C
Hwang
Tae-Sun
TS
Chung
Hyungmin
H
Gwag
Byoung Joo
BJ
Hong
Kwan Soo
KS
Song
Jihwan
J
D000818:Animals; D002465:Cell Movement; D003287:Contrast Media; D003911:Dextrans; D052203:Ferrosoferric Oxide; D006801:Humans; D020244:Infarction, Middle Cerebral Artery / Q000175:diagnosis / Q000473:pathology / Q000628:therapy*; D007501:Iron; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010087:Oxides; D011479:Protamines; D051381:Rats; D017207:Rats, Sprague-Dawley
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D011479:Protamines; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide