Colostrinin decreases hypersensitivity and allergic responses to common allergens.
BACKGROUND
Colostrinin (CLN), isolated from mothers' pre-milk fluid (colostrum), is a uniform mixture of low-molecular-weight, proline-rich polypeptides. CLN induces neurite outgrowth of pheochromocytoma cells, extends the lifespan of diploid fibroblast cells, inhibits beta-amyloid-induced apoptosis and improves cognitive functions when administered to Alzheimer's disease patients.


OBJECTIVE
The aim of this study was to investigate potential allergic responses to CLN and its impact on allergic sensitization and inflammation caused by common allergens.


METHODS
We used a well-characterized mouse model of allergic airway inflammation. Changes in IgE/IgG1 and mucin levels, airway eosinophilia and hyperreactivity to methacholine were determined by ELISA, differential cell counting and whole-body plethysmography, respectively.


RESULTS
CLN did not increase IgE/IgG1 levels or induce cutaneous hypersensitivity reaction, airway inflammation and mucin production. Importantly, CLN significantly (p < 0.001) decreased IgE/IgG1 production, airway eosinophilia, mucin production and hypersensitivity induced by allergenic extracts from ragweed pollen grains and house dust mites.


CONCLUSIONS
CLN itself is non-allergenic; however, it is effective in preventing allergic responses to known indoor and outdoor allergens. These data support the safe application of CLN and its potential use in the prevention of allergic inflammation in humans.
Boldogh
Istvan
I
Department of Microbiology, University of Texas Medical Branch, Galveston, TX 77555, USA. sboldogh@utmb.edu
Aguilera-Aguirre
Leopoldo
L
Bacsi
Attila
A
Choudhury
Barun K
BK
Saavedra-Molina
Alfredo
A
Kruzel
Marian
M
D031164:Ambrosia / Q000276:immunology*; D000818:Animals; D001985:Bronchial Provocation Tests; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006967:Hypersensitivity / Q000097:blood / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000032:analysis / Q000276:immunology; D010455:Peptides / Q000494:pharmacology*; D010993:Plethysmography, Whole Body; D039981:Pyroglyphidae / Q000276:immunology*; D012882:Skin Tests
D007074:Immunoglobulin G; D036341:Intercellular Signaling Peptides and Proteins; D009077:Mucins; D010455:Peptides; C105874:colostrinine; D007073:Immunoglobulin E