title: Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection.
abstract: Toll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral infection, a full-length cDNA of TLR3 (GrTLR3) with a splice variant (GrTLR3s) was identified by homologous cloning and RACE techniques. The antiviral effector molecule Mx gene was cloned and partially sequenced. The mRNA expression levels of GrTLR3, GrTLR3s, and Mx were studied in different tissues before and after virus infection by real-time quantitative RT-PCR. The transcripts of all three genes in liver were significantly increased following GCRV infection (P < 0.05). The mRNA levels in liver were upregulated at 24 h post-injection for GrTLR3 and GrTLR3s, and at 12 h for Mx. The upregulated expression levels were several folds for GrTLR3s, tens of folds for GrTLR3, and hundreds of folds for Mx. By semi-quantitative RT-PCR, GrTLR3 and Mx expressed at all the developmental stages, whereas GrTLR3s could only be detected at later developmental stages. Using RNAi and transgenic techniques, GrTLR3 mediated Mx expression but GrTLR3s did not. The time-dependent upregulation of receptor and effector, and the Mx over-expression dependent on TLR3, indicated that GrTLR3 regulated Mx expression in viral infection through a configuration change in rare minnow, and its splice variant did not contribute to the process.
authors: Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, 430072, People's Republic of China.
authors: Jianguo
authors: J
authors: Su
authors: Zuoyan
authors: Z
authors: Zhu
authors: Yaping
authors: Y
authors: Wang
authors: Jun
authors: J
authors: Zou
authors: Wei
authors: W
authors: Hu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000276:immunology* / Q000821:virology; D005393:Fish Diseases / Q000276:immunology*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary*; D051196:Toll-Like Receptor 3 / Q000502:physiology*
chemical_list: D064547:Myxovirus Resistance Proteins; D051196:Toll-Like Receptor 3; D019204:GTP-Binding Proteins