The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis.
Because lipid rafts are plasma membrane platforms mediating various cellular events such as in signal transduction, immunological response, pathogen invasion, and neurodegenerative diseases, protein identification in the rafts could provide important information to study their function. Here, we present an optimized method to isolate detergent-resistant lipid rafts that are subsequently analyzed by two-dimensional electrophoresis (2-DE). Lipid rafts were isolated based on their two distinct biochemical properties such as Triton X-100 insolubility and low density. To solubilize completely the proteins embedded in lipid rafts, sample lysis buffer (9 M urea, 2 M thiourea, 100 mM DTT, 2% CHAPS (w/v), 60 mM n-octylbeta-D-glucopyranoside, 2% IPG buffer) was applied to the isolated rafts. This method was found to be the most suitable choice for obtaining 2-DE profile of lipid raft proteome from various cells and tissues. We expect that this method could provide the way to dissect the function of raft-associated proteins and to gain a comprehensive insight upon various cellular events mediated through lipid rafts, the specialized domains in cell surface.
Kim
Ki-Bum
KB
Korea University, Seoul, Korea.
Lee
Jae-Seon
JS
Ko
Young-Gyu
YG
D000818:Animals; D003902:Detergents / Q000737:chemistry*; D004229:Dithiothreitol / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D013890:Thiourea / Q000737:chemistry; D014508:Urea / Q000737:chemistry
D003902:Detergents; D008565:Membrane Proteins; D014508:Urea; D013890:Thiourea; D004229:Dithiothreitol