title: Phospholipase C and protein kinase A mediate bradykinin sensitization of TRPA1: a molecular mechanism of inflammatory pain.
abstract: Bradykinin is an inflammatory mediator that plays a pivotal role in pain and hyperalgesia in inflamed tissues by exciting and/or sensitizing nociceptors. TRPA1 is an important component of the transduction machinery through which environmental irritants and endogenous proalgesic agents depolarize nociceptors to elicit inflammatory pain. Here, using electrophysiological, immunocytochemical and behavioural analyses, we showed a functional interaction of these two inflammation-related molecules in both heterologous expressing systems and primary sensory neurons. We found that bradykinin increased the TRPA1 currents evoked by allyl isothiocyanate (AITC) or cinnamaldehyde in HEK293 cells expressing TRPA1 and bradykinin receptor 2 (B2R). This potentiation was inhibited by phospholipase C (PLC) inhibitor or protein kinase A (PKA) inhibitor, and mimicked by PLC or PKA activator. The functional interaction between B2R and TRPA1, as well as the modulation mechanism, was also observed in rat dorsal root ganglia neurons. In an occlusion experiment, the PLC activator could enhance AITC-induced TRPA1 current further even in saturated PKA-mediated potentiation, indicating the additive potentiating effects of the PLC and PKA pathways. These data for the first time indicate that a cAMP-PKA signalling is involved in the downstream from B2R in dorsal root ganglia neurons in addition to PLC. Finally, subcutaneous pre-injection of a sub-inflammatory dose of bradykinin into rat hind paw enhanced AITC-induced pain behaviours, which was consistent with the observations in vitro. Collectively, these results represent a novel mechanism through which bradykinin released in response to tissue inflammation might trigger the sensation of pain by TRPA1 activation.
authors: Department of Anatomy and Neuroscience, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan.
authors: Shenglan
authors: S
authors: Wang
authors: Yi
authors: Y
authors: Dai
authors: Tetsuo
authors: T
authors: Fukuoka
authors: Hiroki
authors: H
authors: Yamanaka
authors: Kimiko
authors: K
authors: Kobayashi
authors: Koichi
authors: K
authors: Obata
authors: Xiuyu
authors: X
authors: Cui
authors: Makoto
authors: M
authors: Tominaga
authors: Koichi
authors: K
authors: Noguchi
mesh_terms: D000818:Animals; D017487:Ankyrins; D001920:Bradykinin / Q000494:pharmacology / Q000502:physiology*; D015220:Calcium Channels / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D017868:Cyclic AMP-Dependent Protein Kinases / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005727:Ganglia, Spinal / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism* / Q000503:physiopathology; D017879:Isothiocyanates / Q000494:pharmacology; D008297:Male; D010146:Pain / Q000378:metabolism* / Q000503:physiopathology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D010738:Type C Phospholipases / Q000502:physiology
chemical_list: D017487:Ankyrins; D015220:Calcium Channels; D017879:Isothiocyanates; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; C480976:Trpa1 protein, rat; C004471:allyl isothiocyanate; D017868:Cyclic AMP-Dependent Protein Kinases; D010738:Type C Phospholipases; D001920:Bradykinin