Mesna protects splanchnic organs from oxidative stress induced by pneumoperitoneum.
BACKGROUND
We investigated the potential beneficial effect of the antioxidant 2-mercaptoethane-sulfonate (mesna) against oxidative stress induced by pneumoperitoneum in splanchnic organs.


METHODS
Wistar rats were subjected to either (a) CO(2) pneumoperitoneum (15 mmHg for 60 min) (group P), (b) pretreatment with mesna (400 mg/kg, p.o.) followed by pneumoperitoneum with a 180 min interval (group MP), (c) sham operation (group S), or (d) administration of mesna only (group M). Forty-five minutes after desufflation (groups P and MP), 60 + 45 min after the induction of anesthesia (group S), or 180 min after mesna administration (group M), tissue specimens were excised from liver, kidneys, jejunum and stomach. Tissue oxidative state was assessed on the basis of glutathione-to-glutathione disulfide ratio, malondialdehyde concentration , and superoxide dismutase activity.


RESULTS
Pneumoperitoneum deteriorated all the oxidative stress markers in the organs studied. Mesna prevented the occurrence of oxidative stress following pneumoperitoneum in all the organs studied. In the absence of pneumoperitoneum, the administration of mesna caused mild enhancement of the oxidative state of liver, stomach, and kidneys compared to sham controls.


CONCLUSIONS
Prophylaxis with mesna prevents oxidative stress induced by pneumoperitoneum in splanchnic organs.
Ypsilantis
Petros
P
Laboratory of Experimental Surgery and Surgical Research, School of Medicine, Democritus University of Thrace, University General Hospital of Alexandroupolis, Greece. pipsil@med.duth.gr
Tentes
Ioannis
I
Anagnostopoulos
Konstantinos
K
Kortsaris
Alexandros
A
Simopoulos
Constantinos
C
D000704:Analysis of Variance; D000818:Animals; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D015080:Mesna / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D015080:Mesna; D019803:Glutathione Disulfide