Pressurized solvent extraction and monolithic column-HPLC/DAD analysis of anthocyanins in red cabbage.
The aim of this work was to develop a fast method for extraction and analysis of anthocyanins in red cabbage. Pressurized hot water containing 5% of ethanol was used as an extremely efficient extraction solvent. HPLC/DAD with a monolithic column was used to accomplish a fast analysis-24 anthocyanin peaks within 18min. Statistical design was used to optimize the studied extraction parameters: temperature (80-120 degrees C); sample amount (1-3g); extraction time (6-11min); concentration of formic acid in the extraction solvent (0-5vol.%). The best extraction conditions for a majority of the anthocyanin peaks were 2.5g of sample, 99 degrees C (at 50bar), 7min of extraction and a solvent composition of water/ethanol/formic acid (94/5/1, v/v/v).
Arapitsas
Panagiotis
P
Uppsala University, Department of Physical and Analytical Chemistry, Analytical Chemistry, P.O. Box 599, 751 24 Uppsala, Sweden.
Turner
Charlotta
C
D000872:Anthocyanins / Q000032:analysis*; D001937:Brassica / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D011312:Pressure; D012997:Solvents; D013696:Temperature
D000872:Anthocyanins; D010936:Plant Extracts; D012997:Solvents