title: Different pattern of pleiotrophin and midkine expression in neuropathic pain: correlation between changes in pleiotrophin gene expression and rat strain differences in neuropathic pain.
abstract: Pleiotrophin (PTN) and midkine (MK) are two growth factors highly redundant in function that exhibit neurotrophic actions and are upregulated at sites of nerve injury, both properties being compatible with a potential involvement in the pathophysiological events that follow nerve damage (i.e. neuropathic pain). We have tested this hypothesis by comparatively studying PTN and MK gene expression in the spinal cord and dorsal root ganglia (DRG) of three rat strains known to differ in their behavioural responses to chronic constriction injury (CCI) of the sciatic nerve: Lewis, Fischer 344 (F344) and Sprague-Dawley (SD). Real time RT-PCR revealed minimal changes in PTN/MK gene expression in the spinal cord after CCI despite the strain considered, but marked changes were detected in DRG. A significant upregulation of PTN gene expression occurred in injured DRG of the F344 strain, the only strain that recovers from CCI-induced mechanical allodynia 28 days after surgery. In contrast, PTN was found to be downregulated in injured DRG of SD rats, the most sensitive strain in behavioural studies. These changes in PTN were not paralleled by concomitant modifications of MK gene expression. The results demonstrate previously unidentified differences between PTN and MK patterns of expression. Furthermore, the data suggest that upregulation of PTN, but not MK, could play an important role in the recovery from CCI.
authors: Department of Molecular and Experimental Medicine and Cell Biology, The Scripps Research Institute, La Jolla, CA, USA.
authors: Laura
authors: L
authors: Ezquerra
authors: Luis F
authors: LF
authors: Alguacil
authors: Trang
authors: T
authors: Nguyen
authors: Thomas F
authors: TF
authors: Deuel
authors: Inmaculada
authors: I
authors: Silos-Santiago
authors: Gonzalo
authors: G
authors: Herradon
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D016207:Cytokines / Q000096:biosynthesis*; D005727:Ganglia, Spinal / Q000473:pathology*; D005786:Gene Expression Regulation; D000078792:Midkine; D009474:Neurons / Q000378:metabolism*; D010146:Pain; D059348:Peripheral Nerve Injuries; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; C066845:pleiotrophin; D000078792:Midkine