Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced Parkinson disease mice.
To determine the survival and differentiation of cultured Human amniotic cells (HACs) upon transplantation into the brain of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson disease (PD) mice. Mouse model of PD was established with injections of MPTP (15 mg/kg, fourth, 2 h interval). After being labeled with PKH26, HACs isolated from human were transplanted into the striatum of PD mice. Immunohistochemistry was performed to evaluate the toxicity of MPTP in the substantia nigra, graft survival and endogenous neurogenesis. Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) level in the striatum were tested by ELISA. Our results showed that cultured HACs can express the marker of neural progenitor cells and differentiate into neuron, dopaminergic neuron, astrocyte and oligodendrocyte. TH-positive neural cells were significantly reduced in the substantia nigra in the model mice, whereas which increased in transplantation mice. Immunohistology results showed that transplanted HACs survived and migrated in the brain of PD model mouse, though no morphological integration was observed. BrdU-positive cells in the Subventricular zone (SVZ) and neurotrophins of the striatum increased in the transplantation mice. The results suggested that transplanted HACs could survive and promote the endogenous neurogenesis of mice, which maybe related to the increased level of neurotrophins of the striatum.
Kong
Xiang-Ying
XY
Department of Pharmacology, School of Basic Medicine, Peking Union Medical College and Institute of Basic Medical Science, Chinese Academy of Medical Sciences, Beijing, 100005 China.
Cai
Zhe
Z
Pan
Lin
L
Zhang
Lan
L
Shu
Jun
J
Dong
Yi-Long
YL
Yang
Nan
N
Li
Qing
Q
Huang
Xiao-Jie
XJ
Zuo
Ping-Ping
PP
D000650:Amnion / Q000166:cytology*; D000818:Animals; D000963:Antimetabolites; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D001973:Bromodeoxyuridine; D002470:Cell Survival / Q000502:physiology; D017690:Cell Transplantation / Q000502:physiology*; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology; D004298:Dopamine / Q000502:physiology; D005455:Fluorescent Antibody Technique; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D020267:MPTP Poisoning / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D009043:Motor Activity / Q000502:physiology; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D009414:Nerve Growth Factors / Q000378:metabolism; D004856:Postural Balance / Q000502:physiology; D016707:Tissue Fixation; D014183:Transplantation, Heterologous; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D000963:Antimetabolites; D019208:Brain-Derived Neurotrophic Factor; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D014446:Tyrosine 3-Monooxygenase; D001973:Bromodeoxyuridine; D004298:Dopamine