title: Membrane and protein dynamics in virus-infected plant cells.
abstract: In terms of functional genomics research, Nicotiana benthamiana, more so than other model plants, is highly amenable to high-throughput methods, especially those employing virus-induced gene silencing and agroinfiltration. Furthermore, through recent and ongoing sequencing projects, there are now upward of 18,000 unique N. benthamiana ESTs to support functional genomics research. Despite these advances, the cell biology of N. benthamiana itself, and in the context of virus infection, lags behind that of other model systems. Therefore, to meet the challenges of diverse cell biology studies that will be derived from ongoing functional genomics projects, a series of methods relevant to the characterization of membrane and protein dynamics in virus-infected cells are provided here. The data presented here were derived from our studies with plant rhabdoviruses. However, the employed techniques should be broadly applicable within the field of plant virology. We report here on the use of a novel series of binary vectors for the transient or stable expression of autofluorescent protein fusions in plants. Use of these vectors in conjunction with advanced microscopy techniques such as fluorescent recovery after photobleaching and total internal fluorescence microscopy, has revealed novel insight into the membrane and protein dynamics of virus-infected cells.
authors: Department of Plant Pathology, University of Kentucky, 201F Plant Science Building, Lexington, KY 40546, USA.
authors: Michael
authors: M
authors: Goodin
authors: Romit
authors: R
authors: Chakrabarty
authors: Sharon
authors: S
authors: Yelton
mesh_terms: D017796:Caulimovirus / Q000472:pathogenicity*; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D018745:Genome, Plant; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses / Q000472:pathogenicity*; D016133:Polymerase Chain Reaction; D011523:Protoplasts / Q000648:ultrastructure / Q000821:virology; D014026:Nicotiana / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins