Application of non-ionic surfactant in the microwave-assisted extraction of alkaloids from Rhizoma Coptidis.
The feasibility of employing non-ionic surfactant oligoethylene glycol monoalkyl ether (Genapol X-080) as an alternative and effective solvent for the microwave-assisted extraction of alkaloids from Rhizoma Coptidis was demonstrated. When compared with commonly used solvents, 5% Genapol X-080 enhanced the extraction efficiency. Under optimal conditions, i.e. 5% acidified Genapol X-080 (v/v), microwave-assisted extraction for 10 min at 100 degrees C, the extraction recovery of alkaloids reached 92.8% in a one-step extraction. The efficiency of cloud-point preconcentration of three alkaloids was in the range 93.6-94.7% with relative standard deviation lower than 3.3% by the proposed procedure. Furthermore, the combination of microwave-assisted extraction and cloud-point extraction was shown to be a green, rapid and effective approach for alkaloids preconcentration of Rhizoma Coptidis samples.
Sun
Chen
C
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
Liu
Huizhou
H
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008823:Micelles; D008872:Microwaves; D015394:Molecular Structure; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D013501:Surface-Active Agents / Q000737:chemistry*
D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D007477:Ions; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents