title: H5N1-infected cells in lung with diffuse alveolar damage in exudative phase from a fatal case in Vietnam.
abstract: Necropsied lung tissues of three fatal cases with avian influenza A virus (H5N1) infection in Vietnam were analyzed to detect H5N1 virus-infected cells. Formalin-fixed and paraffin-embedded lung tissue sections showed typical histological features of diffuse alveolar damage (DAD) in all cases. Immunohistochemistry for the influenza A virus nucleoprotein antigen revealed positive signals of bronchiolar and alveolar epithelial cells in only one patient, who exhibited DAD with an exudative phase and died on the 6th day after onset. However, no signal was detected in the other two cases of DAD with a proliferative phase. These patients died on day 16 and day 17 after onset, respectively. H5N1 virus antigens were detected predominantly in epithelial cells in terminal bronchioles and in alveoli, i.e., type I and type II alveolar pneumocytes, and in alveolar macrophages. The pathogenesis of exudative DAD caused by H5N1 infection is discussed.
authors: The National Hospital of Pediatrics, Hanoi, Vietnam.
authors: Nguyen Thanh
authors: NT
authors: Liem
authors: Noriko
authors: N
authors: Nakajima
authors: Le Phuc
authors: le P
authors: Phat
authors: Yuko
authors: Y
authors: Sato
authors: Hoang Ngoc
authors: HN
authors: Thach
authors: Pham Viet
authors: PV
authors: Hung
authors: Luong Thi
authors: LT
authors: San
authors: Harutaka
authors: H
authors: Katano
authors: Toshio
authors: T
authors: Kumasaka
authors: Teruaki
authors: T
authors: Oka
authors: Shoji
authors: S
authors: Kawachi
authors: Takeji
authors: T
authors: Matsushita
authors: Tetsutaro
authors: T
authors: Sata
authors: Koichiro
authors: K
authors: Kudo
authors: Kazuo
authors: K
authors: Suzuki
mesh_terms: D000956:Antigens, Viral; D002648:Child; D002675:Child, Preschool; D017809:Fatal Outcome; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000302:isolation & purification; D007251:Influenza, Human / Q000473:pathology* / Q000821:virology; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D011650:Pulmonary Alveoli / Q000473:pathology* / Q000821:virology; D014744:Vietnam
chemical_list: D000956:Antigens, Viral