title: Genetic effects of X-ray and carbon ion irradiation in head and neck carcinoma cell lines.
abstract: The effects of X-ray and carbon ion irradiation on DNA and genes in head and neck carcinoma cells were examined. Four head and neck cancer cell lines (squamous cell carcinoma, salivary gland cancer, malignant melanoma, normal keratinocyte) were treated with 1, 4, and 7 GyE of carbon ion, or 1, 4, and 8 Gy of X-ray, respectively. DNA and RNA in the treated cells were extracted and purified. PCR-LOH (polymerase chain reaction-loss of heterozygosity) analysis with 6 microsatellite regions on chromosome 17 was performed to determine DNA structural damage, and then microarray analysis was performed to reveal changes in gene expression. PCR-LOH analysis detected high LOH in cells treated by radiation, indicating that most of the damage by X-ray occurred in the target region on one of the homologous chromosomes. However, carbon ion caused homo-deletion, which means deletion of the counterparts in both homologous chromosomes.
authors: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Chiba, Japan.
authors: Nobuharu
authors: N
authors: Yamamoto
authors: Chihaya
authors: C
authors: Ikeda
authors: Takashi
authors: T
authors: Yakushiji
authors: Takeshi
authors: T
authors: Nomura
authors: Akira
authors: A
authors: Katakura
authors: Takahiko
authors: T
authors: Shibahara
authors: Jun-Etsu
authors: JE
authors: Mizoe
mesh_terms: D002250:Carbon Radioisotopes / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000532:radiotherapy; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D002886:Chromosomes, Human, Pair 17 / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000528:radiation effects*; D017353:Gene Deletion; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects; D019656:Loss of Heterozygosity / Q000528:radiation effects; D008545:Melanoma / Q000235:genetics / Q000532:radiotherapy; D053842:Microsatellite Instability / Q000528:radiation effects; D018895:Microsatellite Repeats / Q000528:radiation effects; D012334:RNA, Neoplasm / Q000528:radiation effects; D019275:Radiopharmaceuticals / Q000627:therapeutic use*; D011879:Radiotherapy Dosage; D012468:Salivary Gland Neoplasms / Q000235:genetics / Q000532:radiotherapy; D017385:Sequence Homology; D014965:X-Rays
chemical_list: D002250:Carbon Radioisotopes; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D019275:Radiopharmaceuticals