title: Interferon-gamma alters electrical activity and clock gene expression in suprachiasmatic nucleus neurons.
abstract: The proinflammatory cytokine interferon (IFN-gamma) is an immunomodulatory molecule released by immune cells. It was originally described as an antiviral agent but can also affect functions in the nervous system including circadian activity of the principal mammalian circadian pacemaker, the suprachiasmatic nucleus. IFN-gamma and the synergistically acting cytokine tumor necrosis factor-alpha acutely decrease spontaneous excitatory postsynaptic activity and alter spiking activity in tissue preparations of the SCN. Because IFN-gamma can be released chronically during infections, the authors studied the long-term effects of IFN-gamma on SCN neurons by treating dispersed rat SCN cultures with IFN-gamma over a 4-week period. They analyzed the effect of the treatment on the spontaneous spiking pattern and rhythmic expression of the "clock gene," Period 1. They found that cytokine-treated cells exhibited a lower average spiking frequency and displayed a more irregular firing pattern when compared with controls. Furthermore, long-term treatment with IFN-gamma in cultures obtained from a transgenic Per1-luciferase rat significantly reduced the Per1-luc rhythm amplitude in individual SCN neurons. These results show that IFN-gamma can alter the electrical properties and circadian clock gene expression in SCN neurons. The authors hypothesize that IFN-gamma can modulate circadian output, which may be associated with sleep and rhythm disturbances observed in certain infections and in aging.
authors: Department of Biology, University of Virginia, Charlottesville, VA, USA.
authors: Yongho
authors: Y
authors: Kwak
authors: Gabriella B
authors: GB
authors: Lundkvist
authors: Johan
authors: J
authors: Brask
authors: Alec
authors: A
authors: Davidson
authors: Michael
authors: M
authors: Menaker
authors: Krister
authors: K
authors: Kristensson
authors: Gene D
authors: GD
authors: Block
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D001683:Biological Clocks / Q000502:physiology; D056926:CLOCK Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D005786:Gene Expression Regulation; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D056950:Period Circadian Proteins; D051381:Rats; D013493:Suprachiasmatic Nucleus / Q000166:cytology* / Q000378:metabolism; D013696:Temperature; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; C539024:Per1 protein, rat; D056950:Period Circadian Proteins; D015534:Trans-Activators; D005680:gamma-Aminobutyric Acid; D007371:Interferon-gamma; D056926:CLOCK Proteins; C538945:Clock protein, rat