Isolation of amyloplasts.
Two different methods for the preparation of starch-rich plastids are described together with protocols for the determination of plastid yield, purity, and intactness. The preparation of amyloplasts from maize endosperm and oilseed rape embryos are given as examples, but the protocols could be adapted for the isolation of starch-rich plastids from other plant organs. A method for the determination of the quantitative distribution of an enzyme between the plastids and cytosol is given. Typical results and references for marker enzymes for a range of subcellular compartments are listed.
Denyer
Kay
K
John Innes Centre, Norwich Research Park, Norwich, Norfolk.
Pike
Marilyn
M
D015415:Biomarkers / Q000032:analysis*; D029688:Brassica napus / Q000196:embryology / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000378:metabolism; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure; D018087:Plastids / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D012639:Seeds / Q000378:metabolism; D013347:Subcellular Fractions; D003313:Zea mays / Q000737:chemistry*
D015415:Biomarkers; D051599:Glucose-1-Phosphate Adenylyltransferase