title: Infection of primary cultures of murine splenic and thymic cells with coxsackievirus B4.
abstract: Infection of primary cultures of total splenic and thymic cells from BALB/c and C3H/HeN mice with CVB4 E2 and JVB strains has been investigated. The presence of positive-strand viral RNA within cells was determined by semi-nested RT-PCR, and viral replication was attested by detection of intracellular negative-strand viral RNA and by release of infectious particles in culture supernatants. Viral replication occurred with both CVB4 strains to an extent dependent on the genetic background of the host. No interferon-alpha production was detected in the supernatants of CVB4-infected cultures using biological titration. Together these results suggest that infection of splenic and thymic cells can play a role in virus dissemination, and therefore in the pathophysiology of CVB4 infections.
authors: Virology Laboratory/UPRES EA3610, Faculty of Medicine, University of Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037 Lille, France.
authors: Hela
authors: H
authors: Jaïdane
authors: Jawhar
authors: J
authors: Gharbi
authors: Pierre-Emmanuel
authors: PE
authors: Lobert
authors: Delphine
authors: D
authors: Caloone
authors: Bernadette
authors: B
authors: Lucas
authors: Famara
authors: F
authors: Sané
authors: Thierry
authors: T
authors: Idziorek
authors: Marie-Bénédicte
authors: MB
authors: Romond
authors: Mahjoub
authors: M
authors: Aouni
authors: Didier
authors: D
authors: Hober
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D008214:Lymphocytes / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000821:virology*; D013950:Thymus Gland / Q000166:cytology / Q000821:virology*
chemical_list: D012367:RNA, Viral