In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice.
The presence of a perforation is known to significantly compromise the outcome of endodontic treatment. One potential use of regenerative endodontic therapy might be the repair of root canal perforations. In addition to nutrients and systemic in situ interactions, the 3 main components believed to be essential for tissue regeneration are stem cells, scaffold, and growth factors. This study investigated the role of each component of the tissue engineering triad in the organization and differentiation of dental pulp stem cells (DPSCs) in a simulated furcal perforation site by using a mouse model. Collagen served as the scaffold, and dentin matrix protein 1 (DMP1) was the growth factor. Materials were placed in simulated perforation sites in dentin slices. Mineral trioxide aggregate was the control repair material. At 6 weeks, the animals were killed, and the perforation sites were evaluated by light microscopy and histologic staining. Organization of newly derived pulp tissue was seen in the group containing the triad of DPSCs, a collagen scaffold, and DMP1. The other 4 groups did not demonstrate any apparent tissue organization. Under the conditions of the present study, it might be concluded that the triad of DPSCs, a collagen scaffold, and DMP1 can induce an organized matrix formation similar to that of pulpal tissue, which might lead to hard tissue formation.
Prescott
Rebecca S
RS
Department of Endodontics, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
Alsanea
Rajaa
R
Fayad
Mohamed I
MI
Johnson
Bradford R
BR
Wenckus
Christopher S
CS
Hao
Jianjun
J
John
Asha S
AS
George
Anne
A
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D003762:Dental Instruments / Q000009:adverse effects; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010750:Phosphoproteins / Q000494:pharmacology*; D012038:Regeneration / Q000187:drug effects*; D018915:Root Canal Preparation / Q000009:adverse effects / Q000295:instrumentation; D033581:Stem Cell Transplantation; D040521:Subcutaneous Tissue; D054457:Tissue Scaffolds; D018677:Tooth Injuries / Q000628:therapy; D014092:Tooth Root / Q000293:injuries
C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D003094:Collagen