title: Detection and species identification of Cryptosporidium from Taiwan feeding animals.
abstract: In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Cryptosporidium spp. oocysts. Ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of Cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. Nucleic acid sequencing of the 18S rRNA gene identified 3 clusters of Cryptosporidium spp. Three Cryptosporidium parvum isolates were from cattle and sheep feces. One Cryptosporidium andersoni isolate was detected from pig feces. The other 2 novel Cryptosporidium genotypes were not similar to any known Cryptosporidium spp. according to the DNA sequences of the 18S rRNA gene.
authors: Department of Earth and Environmental Sciences, National Chung Cheng University, 168, University Road, Min-Hsiung, Chiayi, Taiwan, ROC. bmhsu@eq.ccu.edu.tw
authors: Bing-Mu
authors: BM
authors: Hsu
authors: Hau-Yang
authors: HY
authors: Wun
authors: Chih-Li Lilian
authors: CL
authors: Hsu
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology; D002645:Chickens; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006734:Horse Diseases / Q000453:epidemiology / Q000469:parasitology; D006736:Horses; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000453:epidemiology / Q000469:parasitology; D015800:Protozoan Proteins / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology / Q000469:parasitology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology; D013624:Taiwan / Q000453:epidemiology
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S