Preparation of PVA membrane for immobilization of GOD for glucose biosensor.
A membrane was prepared using polyvinyl alcohol (PVA) with low and high degree of polymerization (DOP), acetone, benzoic acid (BA) and was cross-linked by UV treatment. Membrane composition was optimized on the basis of swelling index. Membrane prepared with 12% low DOP and 8% high DOP of PVA, 2% BA, dissolved in buffer containing 20% acetone and cross-linked with UV treatment exhibited lower swelling index. Fourier transform infrared (FTIR) study of the membranes showed appearance of a strong band at approximately 2337 cm(-1) when UV was used for cross-linking in the presence of benzoic acid. Scanning electron microscope (SEM) study revealed that membrane cross-linked with UV treatment was smoother. Glucose oxidase (GOD)-PVA membrane was associated with the dissolved oxygen (DO) probe for biosensor reading. Glucose was detected on the basis of depletion of oxygen, when immobilized GOD oxidizes glucose to gluconolactone. A wide detection range, 0.9-225 mg/dl was estimated from the linear range of calibration plot of biosensor reading. Membranes were reused for 32 reactions without significant loss of activity and stored for 30 days (approximately 90% activity) at 4 degrees C. Membranes were also used with real blood samples.
Kumar
Jitendra
J
Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
D'Souza
S F
SF
D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006801:Humans; D008567:Membranes, Artificial; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011142:Polyvinyl Alcohol; D005949:Glucose Oxidase; D005947:Glucose