[Reconstruction of three different kinds of tympanic membrane in vitro by tissue engineering technique].
OBJECTIVE
To reconstruct three different kinds of tympanic membrane in vitro by tissue engineering technique, and to examine their histological structures and mechanical properties.


METHODS
The skin and dura of pig (weight 30 kg) were processed with high satuated saline and enzymes to make extracellular matrix. Meanwhile, fibroblasts (1 x 10(6)/mL, 0.2 mL) were seeded on the surface of these two scaffolds and collagen. The composite tissues were cultured in vitro for 1 week and examined in histological structure and mechanical properties.


RESULTS
Fibroblasts cultured were spindle-shaped and could grow and attach to these scaffolds with a arrangement of sarciniform and parallel. The reconstructed tissue of ECM and collagen appeared to integrate well and had better bio-compatibility. The mean thickness of the collagen, the skin and the dura (all covered with fibroblasts) were 9.4, 10.0 and 10.4 microm respectively. The tension of the collagen was (1.417+/-0.030) N/mm2, of the acellular dermal matrix was (24.500+/-2.040) N/mm2 (being close to the tension of normal tympanic membrane, 26.700 N/mm2), of the acellular dura was (53.300+/-2.600) N/mm2.


CONCLUSIONS
The results suggest that the tension and the thinkness of acellular dermal matrix is similar to the normal tympanic membrane of guinea pig, it is an ideal material for tympanoplasty.
Deng
Zhihong
Z
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University Xi'an Shaanxi, 710032, P.R. China. dengzh@fmmu.edu.cn
Tian
Yongsheng
Y
Wang
Jinling
J
Qiu
Jianhu
J
Liu
Yuan
Y
Zhao
Yu
Y
Jin
Yan
Y
D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D004388:Dura Mater / Q000166:cytology; D005240:Feasibility Studies; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014432:Tympanic Membrane / Q000166:cytology* / Q000648:ultrastructure
D001672:Biocompatible Materials; D003094:Collagen