title: Endurance training modulates lymphocyte function in rats with post-MI CHF.
abstract: OBJECTIVE
  Exercise training restores innate immune system cell function in post-myocardial infarction (post-MI) rats. However, studies of the involvement of lymphocyte (Ly) in the setting of the congestive heart failure (CHF) are few. To address this issue, we investigated the function of Ly obtained from cervical lymph nodes from post-MI CHF rats submitted to treadmill running training.
  
  
  METHODS
  Twenty-five male Wistar rats were randomly assigned to the following groups: rats submitted to ligation of the left coronary artery, which were sedentary (MI-S, N = 7, only limited activity) or trained (MI-T, N = 6, on a treadmill (0% grade at 13-20 m.m) for 60 min.d, 5 d.wk, for 8-10 wk); or sham-operated rats, which were sedentary (sham-S, N = 6) or trained (sham-T, N = 6). The incorporation of [2-C]-thymidine by Ly cultivated in the presence of concanavalin A (Con A) and lipopolysaccharide (LPS), cytokine production by Ly cultivated in the presence of phytohemagglutinin (PHA), and plasma concentration of glutamine were assessed in all groups, 48 h after the last exercise session.
  
  
  RESULTS
  Proliferative capacity was increased, following incubation with Con-A in the MI groups, when compared with the sham counterparts. When incubated in the presence of PHA, MI-S produced more IL-4 (96%) than sham-S (P < 0.001). The training protocol induced a 2.2-fold increase in the production of interleukin-2 (P < 0.001) of the cells obtained from the cervical lymph nodes of MI-T, compared with MI-S.
  
  
  CONCLUSIONS
  The moderate endurance training protocol caused an increase in IL-2 production, and a trend toward the reversion of the Th1/Th2 imbalance associated with IL-4 production increased in the post-MI CHF animal model.
authors: Molecular Biology of the Cell Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. migueljr@usp.br
authors: Miguel Luiz
authors: ML
authors: Batista
authors: Ronaldo V T
authors: RV
authors: Santos
authors: Renato D
authors: RD
authors: Lopes
authors: Antônio C
authors: AC
authors: Lopes
authors: Luís Fernando B P
authors: LF
authors: Costa Rosa
authors: Marília C L
authors: MC
authors: Seelaender
mesh_terms: D000818:Animals; D005973:Glutamine; D006333:Heart Failure; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology*; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005973:Glutamine