Antioxidant potential of dimethyl lithospermate isolated from Salvia miltiorrhiza (red sage) against peroxynitrite.
Peroxynitrite (ONOO(-)) is a reactive oxidant formed from superoxide and nitric oxide that can readily oxidize cellular components, including essential protein, non-protein thiols, and DNA. ONOO(-) has contributed to the pathogenesis of diseases such as stroke, heart disease, Alzheimer's disease, and atherosclerosis. In this study, the ability of dimethyl lithospermate (DML), isolated from Salvia miltiorrhiza, to scavenge ONOO(-) and to protect cells against reactive species and ONOO(-) was investigated. The data obtained show that DML can efficiently scavenge native ONOO(-) as well as ONOO(-) derived from the ONOO(-) donor 3-morpholinosydnonimine hydrochloride. Spectrophotometric analysis revealed that DML led to decreased ONOO(-)-mediated nitration of tyrosine through electron donation. DML significantly inhibited nitration of bovine serum albumin by ONOO(-) in a dose-dependent manner. DML also manifested cytoprotection from cell damage induced by ONOO(-). The present study suggests that DML is an effective ONOO(-) scavenger and promotes cellular defense activity in the protection against ONOO(-)-involved diseases.
Kim
Ji Young
JY
College of Pharmacy, Aging Tissue Bank, Pusan National University, Busan, Republic of Korea.
Kim
Hyung Seok
HS
Kang
Hye Sook
HS
Choi
Jae Sue
JS
Yokozawa
Takako
T
Chung
Hae Young
HY
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D002460:Cell Line; D042783:Endothelial Cells; D016166:Free Radical Scavengers / Q000494:pharmacology; D030421:Peroxynitrous Acid / Q000737:chemistry*; D051381:Rats; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry
D000975:Antioxidants; D001572:Benzofurans; D016166:Free Radical Scavengers; C530488:dimethyl lithospermate; D030421:Peroxynitrous Acid; D014443:Tyrosine