title: Suspended-drop electroporation for high-throughput delivery of biomolecules into cells.
abstract: We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.
authors.affiliation: Department of Chemical and Systems Biology, Bio-X Program, Clark Center, 318 Campus Drive, Stanford University School of Medicine, Stanford, California 94305, USA. guignet@stanford.edu
authors.forename: Emmanuel G
authors.initials: EG
authors.lastname: Guignet
authors.forename: Tobias
authors.initials: T
authors.lastname: Meyer
mesh_terms: D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D003911:Dextrans / Q000378:metabolism; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D020868:Gene Silencing; D006801:Humans; D009474:Neurons / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D003911:Dextrans; D034741:RNA, Small Interfering