Interdisciplinary study for the evaluation of biochemical alterations on mussel Mytilus galloprovincialis exposed to a tributyltin-polluted area.
An interdisciplinary approach was employed to monitor the concentration and the effects of butyltin compounds in mussels (Mytilus galloprovincialis). Tissues from animals exposed to a marine area (Vado Ligure harbour) with a high concentration of tributyltin (TBT) were analysed and compared with control samples. TBT concentrations were measured by gas chromatography-mass spectrometry and the protein pattern in gill tissues was studied by proteomic analysis. Several proteomic signatures associated with contaminant exposure were observed; spots that were significantly increased in all contaminated samples were identified by mass spectrometry as fragments of β-tubulin. The degradation of β-tubulin was then confirmed by western blot analysis with specific anti-β-tubulin antibody. The effects observed on mussel gills after exposure in the TBT-polluted area are discussed.
Magi
Emanuele
E
Department of Chemistry and Industrial Chemistry, University of Genoa, 16146, Genoa, Italy. magie@chimica.unige.it
Liscio
Camilla
C
Pistarino
Erika
E
Santamaria
Barbara
B
Di Carro
Marina
M
Tiso
Micaela
M
Scaloni
Andrea
A
Renzone
Giovanni
G
Cosulich
M Elisabetta
ME
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D008401:Gas Chromatography-Mass Spectrometry; D005880:Gills / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014220:Trialkyltin Compounds / Q000032:analysis* / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism*
D014220:Trialkyltin Compounds; D014404:Tubulin; C011559:tributyltin