Neuropeptide Y promotes neurogenesis in murine subventricular zone.
Stem cells of the subventricular zone (SVZ) represent a reliable source of neurons for cell replacement. Neuropeptide Y (NPY) promotes neurogenesis in the hippocampal subgranular layer and the olfactory epithelium and may be useful for the stimulation of SVZ dynamic in brain repair purposes. We describe that NPY promotes SVZ neurogenesis. NPY (1 microM) treatments increased proliferation at 48 hours and neuronal differentiation at 7 days in SVZ cell cultures. NPY proneurogenic properties are mediated via the Y1 receptor. Accordingly, Y1 receptor is a major active NPY receptor in the mouse SVZ, as shown by functional autoradiography. Moreover, short exposure to NPY increased immunoreactivity for the phosphorylated form of extracellular signal-regulated kinase 1/2 in the nucleus, compatible with a trigger for proliferation, whereas 6 hours of treatment amplified the phosphorylated form of c-Jun-NH(2)-terminal kinase signal in growing axons, consistent with axonogenesis. NPY, as a promoter of SVZ neurogenesis, is a crucial factor for future development of cell-based brain therapy. Disclosure of potential conflicts of interest is found at the end of this article.
Agasse
Fabienne
F
Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, Portugal.
Bernardino
Liliana
L
Kristiansen
Heidi
H
Christiansen
Søren H
SH
Ferreira
Raquel
R
Silva
Bruno
B
Grade
Sofia
S
Woldbye
David P D
DP
Malva
João O
JO
D000818:Animals; D002118:Calcium / Q000502:physiology; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002552:Cerebral Ventricles / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D009478:Neuropeptide Y / Q000494:pharmacology*
D009478:Neuropeptide Y; D048049:Extracellular Signal-Regulated MAP Kinases; D002118:Calcium