title: Optimization of UDP-N-acetylmuramic acid synthesis.
abstract: UDP-N-acetylmuramic acid (UDP-MurNAc) is a substrate of MurC, an important enzyme in the intracellular pathway of bacterial peptidoglycan biosynthesis. Various approaches towards preparation of UDP-MurNAc have been published but these synthetic preparations were shown to include many problematic steps. An optimization study with the focus on muramyl phosphate and UMP-morpholidate coupling was performed, resulting in a synthetic procedure enabling robust and easily reproducible production on a multi-gram scale.
authors.affiliation: Drug Discovery Department, Lek Pharmaceuticals, Verovikova 57, 1526 Ljubljana, Slovenia. jan.humljan@sandoz.com
authors.forename: J
authors.initials: J
authors.lastname: Humljan
authors.forename: S
authors.initials: S
authors.lastname: Starcević
authors.forename: V
authors.initials: V
authors.lastname: Car
authors.forename: P
authors.initials: P
authors.lastname: Stefanic Anderluh
authors.forename: D
authors.initials: D
authors.lastname: Kocjan
authors.forename: B
authors.initials: B
authors.lastname: Jenko
authors.forename: U
authors.initials: U
authors.lastname: Urleb
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010766:Phosphorylation; D014538:Uridine Diphosphate N-Acetylmuramic Acid / Q000138:chemical synthesis*
chemical_list: D007202:Indicators and Reagents; D014538:Uridine Diphosphate N-Acetylmuramic Acid