[MLPA technique--principles and use in practice].
MLPA (Multiplex Ligation-dependent Probe Amplification) is a recently introduced method, based on PCR principle, useful for the detection of different genetic abnormalities (aneuploidies, gene deletions/duplications, subtelomeric rearrangements, methylation status etc). The technique is simple, reliable and cheap. We present this method to discuss its importance for a modern genetic service and to underline its multiple advantages.
Rusu
Cristina
C
Disciplina de Genetică Medicală, Facultatea de Medicină, Universitatea de Medicină si Farmacie "Gr.T. Popa", Iaşi.
Sireteanu
Adriana
A
Puiu
Maria
M
Skrypnyk
Cristina
C
Tomescu
E
E
Csep
Katalin
K
Creţ
Victoria
V
Barbarii
Ligia
L
D000782:Aneuploidy; D020732:Cytogenetic Analysis / Q000379:methods*; D019175:DNA Methylation; D017353:Gene Deletion; D020440:Gene Duplication; D015321:Gene Rearrangement; D005820:Genetic Testing; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results