title: Development of electroactive and elastic nanofibers that contain polyaniline and poly(L-lactide-co-epsilon-caprolactone) for the control of cell adhesion.
abstract: In this work, electrically conductive polyaniline (PAni) doped with camphorsulfonic acid (CPSA) is blended with poly(L-lactide-co-epsilon-caprolactone) (PLCL), and then electrospun to prepare uniform nanofibers. The CPSA-PAni/PLCL nanofibers show a smooth fiber structure without coarse lumps or beads and consistent fiber diameters (which range from 100 to 700 nm) even with an increase in the amount of CPSA-PAni (from 0 to 30 wt.-%). However, the elongation at break decreases from 391.54 +/- 9.20% to 207.85 +/- 6.74% when 30% of CPSA-PAni is incorporated. Analysis of the surface of the nanofibers demonstrates the presence of homogeneously blended CPSA-PAni. Most importantly, a four-point probe analysis reveals that electrical properties are maintained in the nanofibers where the conductivity is significantly increased from 0.0015 to 0.0138 S x cm(-1) when the nanofibers are prepared with 30% CPSA-PAni. The cell adhesion tests using human dermal fibroblasts, NIH-3T3 fibroblasts, and C2C12 myoblasts demonstrate significantly higher adhesion on the CPSA-PAni/PLCL nanofibers than pure PLCL nanofibers. In addition, the growth of NIH-3T3 fibroblasts is enhanced under the stimulation of various direct current flows. The CPSA-PAni/PLCL nanofibers with electrically conductive properties may potentially be used as a platform substrate to study the effect of electrical signals on cell activities and to direct desirable cell function for tissue engineering applications.
authors.affiliation: School of Chemical Engineering, College of Engineering, Hanyang University, Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Sung In
authors.initials: SI
authors.lastname: Jeong
authors.forename: In Dong
authors.initials: ID
authors.lastname: Jun
authors.forename: Moon Jae
authors.initials: MJ
authors.lastname: Choi
authors.forename: Young Chang
authors.initials: YC
authors.lastname: Nho
authors.forename: Young Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004563:Electrochemistry / Q000379:methods; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000814:Aniline Compounds; D011091:Polyesters; C416807:polyaniline; C062968:lactide-caprolactone copolymer