Role of invariant natural killer T cells in liver sinusoidal endothelial cell-induced immunosuppression among T cells with indirect allospecificity.
We have previously demonstrated that liver sinusoidal endothelial cells (LSECs) that endocytose portally injected allogeneic cells exert immunosuppressive effects on T cells with indirect allospecificity. In this study, we have demonstrated that invariant natural killer T cells plays a significant role in such immunosuppressive effects induced by LSECs. The endocytic activity of LSECs toward intraportally injected splenocytes from B6 major histocompatibility complex class II-deficient C2ta (C2D) mice was markedly impaired in BALB/c CD1d-deficient mice. The intraportal adoptive transfer of LSECs isolated from BALB/c wild-type mice treated with a portal injection of B6 C2D splenocytes into BALB/c mice significantly prolonged the survival of subsequently transplanted heart allografts; however, the transfer of LSECs isolated from similarly treated BALB/c CD1d-deficient mice did not produce such a survival prolonging effect. These findings indicate that natural killer T cells are required for the LSEC-induced immune modulation of T cells with indirect allospecificity.
Shishida
Masayuki
M
Division of Frontier Medical Science, Department of Surgery, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Ohdan
Hideki
H
Onoe
Takashi
T
Tanaka
Yuka
Y
Igarashi
Yuka
Y
Banshodani
Masataka
M
Asahara
Toshimasa
T
D019264:Adoptive Transfer; D000818:Animals; D004705:Endocytosis; D042783:Endothelial Cells / Q000276:immunology* / Q000502:physiology; D022781:Hepatocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D007108:Immune Tolerance; D007165:Immunosuppression Therapy; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011169:Portal Vein; D013154:Spleen / Q000637:transplantation; D014184:Transplantation, Homologous / Q000276:immunology