Effects of radix adenophorae and cyclosporine A on an OVA-induced murine model of asthma by suppressing to T cells activity, eosinophilia, and bronchial hyperresponsiveness.
The present study is performed to investigate the inhibitory effects of Radix Adenophorae extract (RAE) on ovalbumin-induced asthma murine model. To study the anti-inflammatory and antiasthmatic effects of RAE, we examined the development of pulmonary eosinophilic inflammation and inhibitory effects of T cells in murine by RAE and cyclosporine A (CsA). We examined determination of airway hyperresponsiveness, flow cytometric analysis (FACS), enzyme-linked immunosorbent assay (ELISA), quantitative real time (PCR), hematoxylin-eosin staining, and Masson trichrome staining in lung tissue, lung weight, total cells, and eosinophil numbers in lung tissue. We demonstrated how RAE suppressed development on inflammation and decreased airway damage.
Roh
Seong-Soo
SS
Laboratory of Herbology, College of Oriental Medicine, Daegu Hanny University, Daegu 706060, South Korea.
Kim
Seung-Hyung
SH
Lee
Young-Cheol
YC
Seo
Young-Bae
YB
D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000188:drug therapy / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D016572:Cyclosporine / Q000276:immunology* / Q000494:pharmacology; D016207:Cytokines / Q000276:immunology; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007166:Immunosuppressive Agents / Q000276:immunology* / Q000494:pharmacology / Q000627:therapeutic use; D008168:Lung / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D010936:Plant Extracts / Q000276:immunology* / Q000494:pharmacology / Q000627:therapeutic use; D011657:Pulmonary Eosinophilia / Q000188:drug therapy / Q000276:immunology*
D016207:Cytokines; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D007073:Immunoglobulin E; D016572:Cyclosporine; D010047:Ovalbumin