Synthesis and proteomic activity evaluation of a new isotope-coded affinity tagging (ICAT) reagent.
During recent years, quantitative proteome profiling has taken advantage of incorporating the traditional stable isotope dilution analysis into global scale or discovery-based proteomic experiments that use mass spectrometers as detectors to allow the pairwise study of differently expressed proteins. Quantitative protein analysis by means of the isotope-coded affinity tag (ICAT) method and tandem mass spectrometry (MS) enables the pairwise comparison of protein expression levels in biological samples. Herein, a modified ICAT reagent, named BAA-ICAT (beta-alanine-arm-ICAT) in which the polyether linker is replaced by a more water-soluble polyamide one, was investigated.
Guaragna
Annalisa
A
Dipartimento di Chimica Organica e Biochimica Università di Napoli Federico II Via Cinthia, 4 I-80126 Napoli, Italy. guaragna@unina.it
Amoresano
Angela
A
Pinto
Vittoria
V
Monti
Gianluca
G
Mastrobuoni
Guido
G
Marino
Gennaro
G
Palumbo
Giovanni
G
D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D006574:Heterocyclic Compounds, 2-Ring / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D007553:Isotope Labeling; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry*; D040901:Proteomics; D031161:Pseudoalteromonas / Q000737:chemistry; D012995:Solubility; D053719:Tandem Mass Spectrometry / Q000379:methods; D013997:Time Factors; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*
D000345:Affinity Labels; D001426:Bacterial Proteins; D006574:Heterocyclic Compounds, 2-Ring; D007202:Indicators and Reagents; D011108:Polymers; C532007:beta-alanine-arm-ICAT; D015091:beta-Alanine