Anti-herpesvirus activities of Pseudomonas sp. S-17 rhamnolipid and its complex with alginate.
The rhamnolipid biosurfactant PS-17 and its complex with the polysaccharide alginate, both produced by the Pseudomonas sp. S-17 strain, were studied for their antiviral activity against herpes simplex virus (HSV) types 1 and 2. They significantly inhibited the herpesvirus cytopathic effect (CPE) in the Madin-Darby bovine kidney (MDBK) cell line. The investigations were carried out according to the CPE inhibition assay protocol. The suppressive effect of the compounds on HSV replication was dose-dependent and occurred at concentrations lower than the critical micelle concentration of the surfactant. The 50% inhibitory concentration (IC50) of rhamnolipid PS-17 was 14.5 microg/ml against HSV-1 and 13 microg/ml against HSV-2. The IC50 values of the complex were 435 microg/ml for HSV-1 and 482 microg/ml for HSV-2. The inhibitory effects of the substances were confirmed by measuring the infectious virus yields with the multicycle virus growth experimental design as well: deltalog CCID50 of 1.84-2.0 against the two types of herpes simplex viruses by rhamnolipid PS-17 (20 microg/ml), and a strong reduction of the HSV-2 virus yield under the effect of the alginate complex at a concentration of 450 microg/ml. The results indicate that rhamnolipid PS-17 and its alginate complex may be considered as promising substances for the development of anti-herpetic compounds.
Remichkova
Mimi
M
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia. mimy_remichkova@yahoo.com
Galabova
Danka
D
Roeva
Ivana
I
Karpenko
Elena
E
Shulga
Alexander
A
Galabov
Angel S
AS
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002417:Cattle; D002460:Cell Line; D006017:Glycolipids / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D007668:Kidney / Q000821:virology; D011549:Pseudomonas / Q000737:chemistry* / Q000821:virology; D018139:Simplexvirus / Q000187:drug effects*
D000464:Alginates; D000998:Antiviral Agents; D006017:Glycolipids; C418382:rhamnolipid