title: Gene profiling in human blood leucocytes during recovery from septic shock.
abstract: OBJECTIVE
  To assess blood leucocytes gene profiling during recovery phase of septic shock; to test the relation between encoding gene expression and protein level.
  
  
  METHODS
  Gene expression levels were studied at days 0, 1, 7 and 28 (D0, 1, 7 and 28) on a dedicated microarray of 340 genes involved in inflammatory processes.
  
  
  METHODS
  16-bed intensive care unit, Lariboisière University hospital.
  
  
  METHODS
  Seventeen septic shock patients enrolled when at least one additional organ dysfunction occurred.
  
  
  RESULTS
  Changes over time were compared with D0 via the ratio Dx/D0. The time-related gene expression study showed significant changes in ten genes. Among them, S100A8 and S100A12 had a reduced expression over time compared with D0, whereas CD74's expression increased. The microarray results were validated by RT-qPCR for four genes. The S100A8 plasma levels decrease along recovery in parallel with the gene expression decrease. The CD74 gene expression evolution significantly correlated with HLA-DR monocyte expression.
  
  
  CONCLUSIONS
  These results are the first description of variations in expression of key inflammatory genes in the course of the septic shock recovery period.
authors.affiliation: Lariboisière University Hospital, Assistance Publique - Hôpitaux de Paris, Department of Anesthesiology and Critical Care Medicine, Paris, France. dpayen1234@aol.com
authors.forename: Didier
authors.initials: D
authors.lastname: Payen
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Lukaszewicz
authors.forename: Ioulia
authors.initials: I
authors.lastname: Belikova
authors.forename: Valérie
authors.initials: V
authors.lastname: Faivre
authors.forename: Catherine
authors.initials: C
authors.lastname: Gelin
authors.forename: Stefan
authors.initials: S
authors.lastname: Russwurm
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Launay
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sevenet
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics; D040501:Calgranulin A / Q000097:blood* / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006801:Humans; D007962:Leukocytes; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012772:Shock, Septic / Q000235:genetics* / Q000401:mortality; D016019:Survival Analysis
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D040501:Calgranulin A; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C466441:HLA-DRB1*12:01:01 antigen; D000949:Histocompatibility Antigens Class II; C045084:invariant chain