High-yield production of secreted active proteins by the Pseudomonas aeruginosa type III secretion system.
The Escherichia coli system is the system of choice for recombinant protein production because it is possible to obtain a high protein yield in inexpensive media. The accumulation of protein in an insoluble form in inclusion bodies remains a major disadvantage. Use of the Pseudomonas aeruginosa type III secretion system can avoid this problem, allowing the production of soluble secreted proteins.
Derouazi
M
M
Laboratoire TIMC-IMAG GREPI-TheREx, Université Joseph Fourier-Grenoble 1 UMR CNRS 5525, IFR INSERM 130, Grenoble, France.
Toussaint
B
B
Quénée
L
L
Epaulard
O
O
Guillaume
M
M
Marlu
R
R
Polack
B
B
D001426:Bacterial Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins