title: Effects of a potent antioxidant, platinum nanoparticle, on the lifespan of Caenorhabditis elegans.
abstract: We have shown that platinum nanoparticles (nano-Pt) are a superoxide dismutase (SOD)/catalase mimetic. Various data have shown extension of the Caenorhabditis elegans lifespan by antioxidant treatment. The present study was designed to elucidate the survival benefit conferred by nano-Pt, as compared to the well-known SOD/catalase mimetic EUK-8. At 0.5mM, nano-Pt significantly extended the lifespan of wild-type N2 nematodes and at 0.25 and 0.5mM, nano-Pt recovered the shortened lifespan of the mev-1(kn1) mutant, which is due to excessive oxidative stress. In both instances, EUK-8 at 0.05, 0.5, and 5mM did not extend nematode lifespan. Even when 0.4M paraquat was loaded exogenously, nano-Pt (0.1 and 0.5mM) and EUK-8 (0.5 and 5mM) were effective in rescuing worms. Moreover, 0.5mM nano-Pt significantly reduced the accumulation of lipofuscin and ROS induced by paraquat. We measured the in vitro dose-dependent quenching of O(2)(-) and H(2)O(2), indicating that nano-Pt is a more potent SOD/catalase mimetic than EUK-8. Nano-Pt prolonged the worm lifespan, regardless of thermotolerance or dietary restriction. Taken together, nano-Pt has interesting anti-ageing properties.
authors.affiliation: Department of Integrated Biosciences, University of Tokyo, Chiba, Japan.
authors.forename: Juewon
authors.initials: J
authors.lastname: Kim
authors.forename: Mayumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Takahiko
authors.initials: T
authors.lastname: Shimizu
authors.forename: Takuji
authors.initials: T
authors.lastname: Shirasawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Kajita
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Kanayama
authors.forename: Yusei
authors.initials: Y
authors.lastname: Miyamoto
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000378:metabolism; D008062:Lipofuscin / Q000378:metabolism; D008136:Longevity / Q000187:drug effects*; D053768:Metal Nanoparticles; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress; D010984:Platinum / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D008062:Lipofuscin; D010984:Platinum; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase