Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays.
Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.
Okochi
Mina
M
Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Hayashi
Hiroki
H
Ito
Akira
A
Kato
Ryuji
R
Tamura
Yasuaki
Y
Sato
Noriyuki
N
Honda
Hiroyuki
H
D000939:Epitopes; D015234:HLA-A Antigens / Q000737:chemistry*; D059871:HLA-A24 Antigen; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry*; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry*; D040081:Protein Array Analysis
D000939:Epitopes; D015234:HLA-A Antigens; D059871:HLA-A24 Antigen; D018840:HSP70 Heat-Shock Proteins; D009842:Oligopeptides