title: Edaravone prevents kainic acid-induced neuronal death.
abstract: There is growing evidence that free radical generation may play a key role in the neuronal damage induced by prolonged convulsions. Free radical scavengers are known to inhibit neuronal death induced by exposure to excitotoxins. However, this neuroprotective effect has not been demonstrated with treatment after seizures had been stopped. We investigated whether 3-methyl-1-phenyl-2-pyrazolin-5-one, edaravone (Ed), a newly developed free radical scavenger that has been used clinically to treat cerebral infarction, could prevent neuronal loss when administered after the occurrence of seizures in a kainic acid (KA)-induced seizure model. Compared with KA alone, cell loss was significantly reduced when animals received Ed (10 mg/kg i.v.) just after seizures, and when Ed was administered both 60 min before (30 mg/kg i.p.) and after KA injection. Combined before-and-after treatment with Ed significantly ameliorated the KA-induced decrease of glutathione and blocked the KA-induced increase of 4-hydroxy-2-nonenal (HNE). Because before-and-after treatment with Ed significantly lessened the KA-induced increase of HNE, Ed may exert its neuroprotective effect by inhibiting lipid peroxidation. However, post-treatment with Ed prevented neuronal cell loss, while HNE and glutathione levels did not differ from those in animals without Ed, so a mechanism other than free radical scavenging must be involved in the prevention of cell loss. Patients who develop status epilepticus are unlikely to receive adequate antioxidant therapy before the onset, so it is an advantage that Ed can prevent neuronal death even when administered after seizures.
authors.affiliation: Department of Pediatrics, Osaka Medical College, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Miyamoto
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shimakawa
authors.forename: Shuhei
authors.initials: S
authors.lastname: Suzuki
authors.forename: Tohru
authors.initials: T
authors.lastname: Ogihara
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tamai
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D016923:Cell Death / Q000187:drug effects; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D000077553:Edaravone; D004827:Epilepsy / Q000139:chemically induced / Q000150:complications* / Q000503:physiopathology; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D007608:Kainic Acid / Q000037:antagonists & inhibitors; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009410:Nerve Degeneration / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D009498:Neurotoxins / Q000037:antagonists & inhibitors; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000139:chemically induced / Q000150:complications* / Q000503:physiopathology; D016896:Treatment Outcome
chemical_list: D000447:Aldehydes; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D009498:Neurotoxins; D005978:Glutathione; C027576:4-hydroxy-2-nonenal; D000077553:Edaravone; D007608:Kainic Acid; D000983:Antipyrine