title: Activation properties of GAGA transcription factor.
abstract: GAGA is a Drosophila transcription factor that has been involved in many nuclear activities. We present evidence that GAGA factor enhances transcription by stabilizing pre-initiation complex (PIC) and promoting reinitiation. Formation of PIC prior to GAGA addition prevents activation suggesting that GAGA is required early in the formation of activated complexes. GAGA stimulation of transcription can be attributed in part to a stabilization of PIC. All these properties depend on the GAGA C-terminal glutamine-rich domain and, in addition to other roles and together with previous data, support a role of GAGA as a transcription factor.
authors.affiliation: Departament de Biologia Molecular i Cellular Institut de Biologia Molecular de Barcelona, CSIC Parc Científic de Barcelona Josep Samitier, 1-5 08028 Barcelona, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Vaquero
authors.forename: Marta
authors.initials: M
authors.lastname: Blanch
authors.forename: M Lluïsa
authors.initials: ML
authors.lastname: Espinás
authors.forename: Jordi
authors.initials: J
authors.lastname: Bernués
mesh_terms: D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D029721:Drosophila Proteins / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D017434:Protein Structure, Tertiary; D016385:TATA Box; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C063460:Trl protein, Drosophila