title: Increased plasma and optic nerve levels of IL-6, TNF-alpha, and MIP-2 following induction of ischemic optic neuropathy in mice.
abstract: OBJECTIVE
  To investigate levels of proinflammatory cytokines in a mouse model of anterior ischemic optic neuropathy (rAION).
  
  
  METHODS
  AION was induced in C57/BL6 mice and levels of IL-6, TNF-alpha, and MIP-2 were measured in plasma by ELISA and in the optic nerves by RT-PCR at predetermined intervals.
  
  
  RESULTS
  Plasma: IL-6 levels were elevated immediately after rAION induction and decreased gradually thereafter. TNF-alpha showed an early peak on day 1 and again from day 21. MIP-2 levels were increased until day 7. Optic nerve: IL-6, TNF-alpha, and MIP-2 levels increased within a few hours and then decreased gradually. IL-6 had a second peak on day 3.
  
  
  CONCLUSIONS
  Proinflammatory cytokines may play a role in the pathogenesis of rAION.
authors.affiliation: The Krieger Eye Research Laboratory, Felsenstein Medical Research Center, Petach Tikva, Israel.
authors.forename: Bat Chen R
authors.initials: BC
authors.lastname: Avraham
authors.forename: Gad
authors.initials: G
authors.lastname: Dotan
authors.forename: Murat
authors.initials: M
authors.lastname: Hasanreisoglu
authors.forename: Michal
authors.initials: M
authors.lastname: Kramer
authors.forename: Yehudit
authors.initials: Y
authors.lastname: Monselise
authors.forename: Yoram
authors.initials: Y
authors.lastname: Cohen
authors.forename: Dov
authors.initials: D
authors.lastname: Weinberger
authors.forename: Nitza
authors.initials: N
authors.lastname: Goldenberg-Cohen
mesh_terms: D000818:Animals; D054426:Chemokine CXCL2 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009900:Optic Nerve / Q000378:metabolism*; D018917:Optic Neuropathy, Ischemic / Q000097:blood / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism*
chemical_list: D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha