Distribution of 3-hydroxy oxylipins and acetylsalicylic acid sensitivity in Cryptococcus species.
Using a well tested antibody specific for 3-hydroxy oxylipins, we mapped the presence of these oxylipins in selected Cryptococcus (Filobasidiella) species. Immunofluorescence microscopy studies revealed that these compounds are deposited on cell wall surfaces, appendages, and collarettes. In vitro studies revealed that growth of Cryptococcus species was inhibited by acetylsalicylic acid (which is known to inhibit mitochondrial function, including the production of 3-hydroxy oxylipins) at concentrations as low as 1 mmol/L. The results suggest that acetylsalicylic acid is effective in controlling the growth of tested pathogens, probably by targeting their mitochondria. This study further expands the known function of this anti-inflammatory drug as anti-fungal agent.
Sebolai
Olihile M
OM
Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Nelson Mandela Drive, P.O. Box 339, Bloemfontein, Free State 9301, South Africa.
Pohl
Carolina H
CH
Botes
Piet J
PJ
van Wyk
Pieter W J
PW
Mzizi
Refilwe
R
Swart
Chantel W
CW
Kock
Johan L F
JL
D000935:Antifungal Agents / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry; D003454:Cryptococcus / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D013058:Mass Spectrometry; D008853:Microscopy; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D054883:Oxylipins / Q000032:analysis*
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D054883:Oxylipins; D001241:Aspirin