[Nucleotide excision repair in mammalia: mechanism of a primary damage recognition].
Nucleotide excision repair is one of the most important pathways of DNA repair in eukaryotic cells. Defects of this system lead to serious diseases including certain kinds of cancer. Nucleotide excision repair is able to remove a wide range of the structurally diverse DNA damages such as UV induced pyrimidine dimers, bulky chemical adducts arising under the action of carcinogenic compounds or chemotherapeutical drugs on cellular DNA. A broad substrate specificity of this repair pathway is related to the main intriguing question that is the mechanism of damage recognition by the protein complex in the context of the large excess of undamaged DNA. This review is detailed on the key stage of nucleotide excision repair--the recognition of a lesion in DNA, which is still most debated. We have considered the main models of a primary damage recognition and preincision complex formation that have been suggested by the leading groups in this field. Data presented allow to suggest the model of sequential loading of the proteins of reparative complex on damage DNA as the most reasonable.
Rechkunova
N I
NI
Mal'tseva
E A
EA
Lavrik
O I
OI
D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D005057:Eukaryotic Cells / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism; D011740:Pyrimidine Dimers / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects
D002273:Carcinogens; D046912:Multiprotein Complexes; D011740:Pyrimidine Dimers