Gene expression profile analysis of the peripheral blood mononuclear cells from tolerant living-donor liver transplant recipients.
Induction of transplant tolerance is a clinically desirable goal. To provide unbiased insight into transplant tolerance, we analyzed gene expression profiling in peripheral blood mononuclear cells from recipients of living-donor liver transplants (LDLTs) who had retained an immune tolerance with a well-functioning graft for several years using cDNA microarray. The comparative analyses with nontransplanted normal healthy volunteers showed that the majority of reliable detected genes were similar, and 5.6% of the genes in the tested genome (of which 627 up-regulated and 90 down-regulated) were significantly regulated and specific to tolerant LDLT recipients, indicating a significant genetic feature for inducing and maintaining immune tolerance. Moreover, the expression of several selected genes was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction, which correlated to microarray data. Our data indicated that cDNA microarray technology was useful for this application and provided many informative insights into transplant tolerance mechanism.
Kawasaki
Mikiko
M
Laboratory of Transplantation Immunology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan.
Iwasaki
Minoru
M
Koshiba
Takaaki
T
Fujino
Masayuki
M
Hara
Yoshiaki
Y
Kitazawa
Yusuke
Y
Kimura
Hiromitsu
H
Uemoto
Shinji
S
Li
Xiao-Kang
XK
Tanaka
Koichi
K
D001773:Blood Cells / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics* / Q000276:immunology; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D016031:Liver Transplantation / Q000276:immunology*; D019520:Living Donors; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis