title: Airway remodeling is correlated with obstruction in children with severe asthma.
abstract: BACKGROUND
  Severe asthma may involve an irreversible obstructive pattern, and structural changes in bronchial airways are believed to play a key role in this context. The aim of the present study was to compare airway remodeling in severe asthmatic children with or without obstructive pattern.
  
  
  METHODS
  Two groups of children with severe asthma and persistent symptoms, 5-14 years old were included, 15 with persistent obstructive pattern (group O) and 10 without obstructive pattern (group N). Persistent obstructive pattern was defined as a forced expiratory volume in 1 s (FEV(1)) less than 80% of the predicted value after a course of systemic corticosteroids and no significant improvement after bronchodilator. We examined bronchial biopsies by pathological and immunochemical methods and quantified airway smooth muscle (ASM) and mucus gland areas, reticular basement membrane (RBM) thickening, distance between ASM and RBM, muscle light chain kinase (MLCK) expression and number of vessels (CD31 expression).
  
  
  RESULTS
  Surface area of ASM (P = 0.009), MLCK expression (P = 0.03) and number of vessels (P = 0.0008) were increased in group O compared with group N. Distance of RBM-ASM was shorter in group O (P = 0.007). FEV(1) negatively correlated with ASM area (r = -0.6; P = 0.002), MLCK expression (r = -0.45; P = 0.02) and CD31 expression (r = -0.7; P = 0.0003), and positively correlated with the distance of RBM-ASM (r = 0.5; P = 0.007).
  
  
  CONCLUSIONS
  Structural abnormalities of airway remodeling are present in children with severe asthma. Only an increase in surface area of ASM and the density of the vascular network are more pronounced in children with persistent obstructive pattern, while RBM thickening is similar. These results are concordant with longitudinal studies which emphasize the precocity of bronchial obstruction.
authors.affiliation: Unité INSERM U 774, Institut Pasteur de Lille, Lille, France.
authors.forename: I
authors.initials: I
authors.lastname: Tillie-Leblond
authors.forename: J
authors.initials: J
authors.lastname: de Blic
authors.forename: F
authors.initials: F
authors.lastname: Jaubert
authors.forename: B
authors.initials: B
authors.lastname: Wallaert
authors.forename: P
authors.initials: P
authors.lastname: Scheinmann
authors.forename: P
authors.initials: P
authors.lastname: Gosset
mesh_terms: D000293:Adolescent; D000402:Airway Obstruction / Q000503:physiopathology*; D001249:Asthma / Q000473:pathology* / Q000503:physiopathology*; D001706:Biopsy; D001980:Bronchi / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology; D020545:Respiratory Mucosa / Q000473:pathology; D012720:Severity of Illness Index