Ganoderma applanatum: a promising mushroom for antitumor and immunomodulating activity.
The antitumor effect of exo-biopolymer (EXP) produced by Ganoderma applanatum was investigated using sarcoma-180 bearing mice. EXP, when administered (10-80 mg/kg body weight: BW) intraperitoneally, significantly inhibited the growth of solid tumor and increased the natural killer (NK) cell activity. A dose of 40 mg/kg BW was found to be highly effective, as it reduced the tumor formation by 39.7%, and increased the NK cell activity of splenocytes by 51.6% compared with the control group. The complement activity of EXP was increased in accordance with an increase in concentration. The phosphatase activity of macrophages was increased by 0.7-fold (200 microg/mL) compared with the control group. This EXP contained 58.9% carbohydrate and 17.1% protein. The major sugar of EXP was composed of mannose and glucose, while the protein mainly consisted of serine, glycine and aspartic acid.
Jeong
Yong-Tae
YT
Department of Biotechnology, Daegu University, Gyeongbuk, Gyeongsan 712-714, Korea. chsong@daegu.ac.kr
Yang
Byung-Keun
BK
Jeong
Sang-Chul
SC
Kim
Sang-Min
SM
Song
Chi-Hyun
CH
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001704:Biopolymers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D032902:Ganoderma / Q000737:chemistry*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D008517:Phytotherapy; D012510:Sarcoma 180 / Q000473:pathology / Q000517:prevention & control*
D000970:Antineoplastic Agents; D001704:Biopolymers; D007155:Immunologic Factors; D010744:Phosphoric Monoester Hydrolases