[Determination of mexiletine in human blood by liquid chromatography-tandem mass spectrometry].
OBJECTIVE
To establish a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detection of mexiletine by liquid chromatography tandem mass spectrometry.


METHODS
After simple protein precipitation of the blood sample with acetonitrile, the organic solvent layer diluted with LC mobile solvent was separated by Allure PFP Propyl column, confirmed and quantified by MS/MS in the multi-reaction monitoring (MRM) mode via positive electrospray ionization.


RESULTS
Mexiletine and naloxone (internal standard) got ideal resolution under the selected analytical condition. The correlation coeficient of linear calibration curve was over 0.9999 within the mexiletine concentration range 0.02-10 microg/mL. The relative standard deviations were under 10% for intra-day and under 15% for inter-day, and the detection limit was 0.01 microg/mL.


CONCLUSIONS
The established LC-MS/MS method is simple, rapid, sensitive, unaffected by matrix effect and appropriate for detection of mexiletine in blood in the field of therapeutic drug monitoring and forensic toxicology.
Yan
Hui
H
Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China.
Xiang
Ping
P
Bo
Juni
J
Shen
Min
M
D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000737:chemistry; D005554:Forensic Medicine; D006801:Humans; D008801:Mexiletine / Q000097:blood* / Q000737:chemistry / Q000506:poisoning; D015394:Molecular Structure; D009270:Naloxone / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000889:Anti-Arrhythmia Agents; D008801:Mexiletine; D009270:Naloxone