title: Silencing preBötzinger complex somatostatin-expressing neurons induces persistent apnea in awake rat.
abstract: Delineating neurons that underlie complex behaviors is of fundamental interest. Using adeno-associated virus 2, we expressed the Drosophila allatostatin receptor in somatostatin (Sst)-expressing neurons in the preBötzinger Complex (preBötC). Rapid silencing of these neurons in awake rats induced a persistent apnea without any respiratory movements to rescue their breathing. We hypothesize that breathing requires preBötC Sst neurons and that their sudden depression can lead to serious, even fatal, respiratory failure.
authors.affiliation: Department of Neurobiology, Box 951763, David Geffen School of Medicine at University of California Los Angeles, 650 Charles E. Young Dr. South, Los Angeles, California 90095, USA.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Tan
authors.forename: Wiktor A
authors.initials: WA
authors.lastname: Janczewski
authors.forename: Paul
authors.initials: P
authors.lastname: Yang
authors.forename: Xuesi M
authors.initials: XM
authors.lastname: Shao
authors.forename: Edward M
authors.initials: EM
authors.lastname: Callaway
authors.forename: Jack L
authors.initials: JL
authors.lastname: Feldman
mesh_terms: D000818:Animals; D001049:Apnea / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology*; D001683:Biological Clocks / Q000187:drug effects / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D000229:Dependovirus / Q000235:genetics; D004330:Drosophila; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D009415:Nerve Net / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009433:Neural Inhibition / Q000187:drug effects / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology; D010507:Periodicity; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000235:genetics*; D018040:Receptors, Neurokinin-1 / Q000378:metabolism; D018013:Receptors, Neuropeptide / Q000096:biosynthesis / Q000235:genetics*; D012125:Respiratory Center / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012143:Respiratory Physiological Phenomena; D013004:Somatostatin / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D014851:Wakefulness / Q000502:physiology
chemical_list: C096426:AstA-R1 protein, Drosophila; D015415:Biomarkers; D029721:Drosophila Proteins; D009479:Neuropeptides; D043562:Receptors, G-Protein-Coupled; D018040:Receptors, Neurokinin-1; D018013:Receptors, Neuropeptide; C485184:enhanced green fluorescent protein; C076523:allatostatin; D049452:Green Fluorescent Proteins; D013004:Somatostatin