title: Semisynthesis of dimeric proteins by expressed protein ligation.
abstract: A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
authors.affiliation: Università di Napoli Federico II, Dipartimento delle Scienze Biologiche, Facoltà di Scienze Biotecnologiche, via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ziaco
authors.forename: Soccorsa
authors.initials: S
authors.lastname: Pensato
authors.forename: Luca D
authors.initials: LD
authors.lastname: D'Andrea
authors.forename: Ettore
authors.initials: E
authors.lastname: Benedetti
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D019281:Dimerization; D008024:Ligands; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D008024:Ligands; D010455:Peptides; D011506:Proteins; D013438:Sulfhydryl Compounds