Transcription factor assembly on the nicotinic receptor beta4 subunit gene promoter.
Nicotinic acetylcholine receptors are involved in a plethora of fundamental biological processes ranging from muscle contraction to formation of memories. The receptors are pentameric proteins whose subunits are encoded by distinct genes. Subunit composition of a mature nicotinic receptor is governed in part by the transcriptional regulation of each subunit gene. Here, using chromatin immunoprecipitation assays, we report the interaction of the transcription factors Sp1, Sp3, c-Jun and Sox10 with the beta4 subunit gene promoter in neuronal-like cell lines and rodent brain tissue. Our results corroborate previous in-vitro data demonstrating that these transcription factors interact with the beta4 promoter. Taken together, these data suggest that Sp1, Sp3, c-Jun and Sox10 regulate expression of the beta4 subunit gene in the mammalian brain.
Scofield
Michael D
MD
Department of Psychiatry, University of Massachusetts Medical School, Worcester, Massachusetts 01604, USA.
Brüschweiler-Li
Lei
L
Mou
Zhongming
Z
Gardner
Paul D
PD
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000502:physiology*; D002460:Cell Line; D005786:Gene Expression Regulation; D047468:Immunoprecipitation; D051379:Mice; D009474:Neurons / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D011978:Receptors, Nicotinic; D014157:Transcription Factors