title: Isolation and identification of cytotoxic compounds from the wood of Pinus resinosa.
abstract: Methanol extracts of wood from Pinus resinosa were found to be selectively cytotoxic against human lung carcinoma cells, A549 (IC50 41 +/- 6 microg/mL), human colorectal adenocarcinoma cells, DLD-1 (IC50 47 +/- 4 microg/mL) in comparison with healthy cells, WS1 (IC50 130 +/- 11 microg/mL). Five known compounds were isolated and identified by 1H, 13C NMRspectroscopy and HR-ESI-MS mass spectrometry as, pinosylvin monomethyl ether (1), pinosylvin (2), pinosylvin dimethyl ether (3), pinobanksin (4) and (-)-norachelogenin (5). Compound 4 was isolated for the first time in P. resinosa. The cytotoxicity of compounds 1-5 was evaluated against A549, DLD-1 and WS1. Compound 1 exhibited the strongest cytotoxicity against both tumor cell lines and the healthy cell line with an IC50 of 25 +/- 4 microm for A549, 20 +/- 1 microm for DLD-1 and 34 +/- 3 microm for WS1.
authors.affiliation: Laboratoire LASEVE, Université du Québec à Chicoutimi, Chicoutimi (Québec), Canada.
authors.forename: François
authors.initials: F
authors.lastname: Simard
authors.forename: Jean
authors.initials: J
authors.lastname: Legault
authors.forename: Serge
authors.initials: S
authors.lastname: Lavoie
authors.forename: Vakhtang
authors.initials: V
authors.lastname: Mshvildadze
authors.forename: André
authors.initials: A
authors.lastname: Pichette
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005347:Fibroblasts / Q000187:drug effects; D044950:Flavanones / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D009369:Neoplasms / Q000188:drug therapy; D028223:Pinus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000494:pharmacology; D014934:Wood / Q000737:chemistry*; D000094162:Phytoalexins
chemical_list: D000970:Antineoplastic Agents; D044950:Flavanones; D000432:Methanol; D010936:Plant Extracts; D013267:Stilbenes; C108261:pinobanksin; C049032:pinosylvin; D000094162:Phytoalexins