title: Separation of DNA by capillary electrophoresis.
abstract: This chapter reports an overview of the analytical techniques used to perform genetic analysis of polymerase chain reaction products using capillary electrophoresis. Three separate but related techniques are described: the separation of native DNA with detection using fluorescent intercalating dyes, the separation of denatured DNA using fluorescently labeled primers, and the detection of single-strand conformation polymorphisms using denatured DNA separated under native conditions. The various techniques involve electrokinetic injection of the DNA onto a narrow band at the head of the column, sieving the DNA through various entangled polymer matrices, and detection via single or multichannel laser-induced fluorescence. Analytical protocols are provided, and a series of representative electropherograms are included.
authors.affiliation: Department of Chemistry, Florida International University, University Park, Miami, FL, USA.
authors.forename: Bruce
authors.initials: B
authors.lastname: McCord
authors.forename: Brittany
authors.initials: B
authors.lastname: Hartzell-Baguley
authors.forename: Stephanie
authors.initials: S
authors.lastname: King
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D009691:Nucleic Acid Denaturation; D010957:Plasmids / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012015:Reference Standards; D012988:Soil Microbiology
chemical_list: D004275:DNA, Ribosomal; D004247:DNA