Adenoviral-mediated up-regulation of Otos, a novel specific cochlear gene, decreases cisplatin-induced apoptosis of cultured spiral ligament fibrocytes via MAPK/mitochondrial pathway.
Previous reports have implicated Otos, a novel specific gene expressed by spiral ligament fibrocytes (SLFs) with unclear functions, as a protective gene for cochlea. However, whether Otos gene could protect SLFs against cisplatin (DDP)-induced apoptosis remains largely unknown. In the present study, we utilized Adenoviral-mediated gene transfection to up-regulate Otos expression in cultured SLFs and further assessed the cell viability and apoptosis as well as possible MAPK and mitochondrial pathways. As expected, the data showed that Otos up-regulation significantly decreased apoptosis of SLFs induced by DDP possibly through activation of ERK and partial inhibition of JNK and mitochondrial pathway but not p-38 pathway, suggesting Otos as a potential protective gene for cochlea and raising the possibility of Otos up-regulation as a promising approach to DDP-induced deafness therapy.
Zhuo
Xian-Lu
XL
Department of Otolaryngology, Southwest Hospital, Third Military Medical University, Chongqing, China.
Wang
Yan
Y
Zhuo
Wen-Lei
WL
Zhang
Yun-Song
YS
Wei
Yun-Jun
YJ
Zhang
Xue-Yuan
XY
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis; D002478:Cells, Cultured; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000166:cytology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D051381:Rats; D014162:Transfection; D015854:Up-Regulation
D000970:Antineoplastic Agents; C503047:Otos protein, rat; D011506:Proteins; D020928:Mitogen-Activated Protein Kinases; D002945:Cisplatin