Modulation of steroidogenesis and xenobiotic biotransformation responses in zebrafish (Danio rerio) exposed to water-soluble fraction of crude oil.
The induction of CYP enzyme activities, particularly CYP1A1, through the aryl hydrocarbon receptor (AhR) in most vertebrate species is among the most studied biochemical response to planar and aromatic organic contaminant exposure. Since P450 families play central roles in the oxidative metabolism of a wide range of exogenous and endogenous compounds, interactions between the biotransformation processes and reproductive physiological responses are inevitable. Steroidogenesis is the process by which specialized cells in specific tissues, such as the gonad, brain (neurosteroids) and kidney, synthesize steroid hormones. In the present study, we evaluated the effects of water-soluble fraction (WSF) of crude oil on the xenobiotic biotransformation and steroidogenic processes in the head (brain) and whole-body tissue of a model species by transcript analysis using quantitative (real-time) polymerase chain reaction (qPCR), enzyme activities and steroid hormone (testosterone: T and 17beta-estradiol: E2) levels using enzyme immune assay (EIA). Our data showed that exposure of fish to WSF produced an apparent concentration-specific increase of AhR1, CYP1A1 and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) mRNA levels, and decrease of AhR2. On the activity level, WSF produced concentration-specific increase of ethoxyresorufin O-deethylase (EROD), benzyloxyresorufin (BROD) methoxyresorufin (MROD) and pentoxyresorufin (PROD) activities in whole-body tissue. In the steroidogenic pathway, WSF exposure produced apparent concentration-specific decrease of ER* and ERbeta, steroidogenic acute regulatory (StAR) protein, cytochrome P450 side-chain cleavage (P450scc), P450aromA and P450aromB mRNA expression. For steroid hormones, while T levels decreased, E2 levels increased in an apparent WSF concentration-specific manner. In general, the xenobiotic biotransformation and estrogenic responses showed negative relationship after exposure of zebrafish to WSF, suggesting an interaction between these physiological pathways. The relationship between WSF mediated changes in brain StAR, P450scc, 3beta-HSD, ER*alpha, ERbeta, P450aromA, P450aromB and whole-body steroid hormone levels suggests that the experimental animals might be experiencing altered neurosteroidogenesis probably through increased activity level of the biotransformation system. Thus, these responses might represent sensitive diagnostic tools for short-term and acute exposure of fish or other aquatic organisms to WSF.
Arukwe
Augustine
A
Department of Biology, Norwegian University of Science and Technology NTNU, Høgskoleringen 5, 7491 Trondheim, Norway. arukwe@bio.ntnu.no
Nordtug
Trond
T
Kortner
Trond M
TM
Mortensen
Anne S
AS
Brakstad
Odd Gunnar
OG
D000818:Animals; D001711:Biotransformation; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009664:Norway; D010578:Petroleum / Q000032:analysis*; D012995:Solubility; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics; D015262:Xenobiotics / Q000032:analysis / Q000493:pharmacokinetics; D015027:Zebrafish / Q000378:metabolism*
D012739:Gonadal Steroid Hormones; D010578:Petroleum; D014874:Water Pollutants, Chemical; D015262:Xenobiotics