title: Mapping the binding site of thiopeptide antibiotics by proximity-induced covalent capture.
abstract: Proximity-induced covalent capture (PICC) has been established for the investigation of ligand binding to composite protein/oligonucleotide target complexes. The RNA-induced attachment of the thiopeptides Thiostrepton and Nosiheptide to engineered Cys mutants of the ribosomal protein L11 was highly position selective and allowed mapping of their binding site at amino acid resolution.
authors.affiliation: Fakultät Chemie, Technische Universität Dortmund, Otto-Hahn-Str. 6, D-44221 Dortmund, Germany.
authors.forename: Sascha
authors.initials: S
authors.lastname: Baumann
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schoof
authors.forename: Surendra D
authors.initials: SD
authors.lastname: Harkal
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Arndt
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D003545:Cysteine / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D008024:Ligands; D009841:Oligonucleotides; D010455:Peptides; D012269:Ribosomal Proteins; D013438:Sulfhydryl Compounds; C027624:ribosomal protein L11; D003545:Cysteine