Oseltamivir enhances hippocampal network synchronization.
Oseltamivir, a widely used anti-influenza drug, inhibits virus neuraminidase. A mammalian homologue of this enzyme is expressed in the brain, yet the effect of oseltamivir on central neurons is largely unknown. Patch-clamp recordings ex vivo revealed that oseltamivir enhanced spike synchronization between hippocampal CA3 pyramidal cells. Time-lapse multineuron calcium imaging revealed that oseltamivir and its active metabolite evoked synchronized population bursts that recruited virtually all neurons in the network. This unique, so-far-unknown, event was attenuated by muscarinic receptor antagonist. Thus, oseltamivir is a useful tool for investigating a new aspect of neural circuit operation.
Usami
Atsushi
A
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Sasaki
Takuya
T
Satoh
Nobuhiro
N
Akiba
Takahiro
T
Yokoshima
Satoshi
S
Fukuyama
Tohru
T
Yamatsugu
Kenzo
K
Kanai
Motomu
M
Shibasaki
Masakatsu
M
Matsuki
Norio
N
Ikegaya
Yuji
Y
D000200:Action Potentials; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D008856:Microscopy, Fluorescence; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009415:Nerve Net / Q000187:drug effects* / Q000201:enzymology; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D053139:Oseltamivir / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D046509:Tissue Culture Techniques
D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D018727:Muscarinic Antagonists; D053139:Oseltamivir; D009439:Neuraminidase