title: Detection of active butyrate-degrading microorganisms in methanogenic sludges by RNA-based stable isotope probing.
abstract: Butyrate-degrading bacteria in four methanogenic sludges were studied by RNA-based stable isotope probing. Bacterial populations in the (13)C-labeled rRNA fractions were distinct from unlabeled fractions, and Syntrophaceae species, Tepidanaerobacter sp., and Clostridium spp. dominated. These results suggest that diverse microbes were active in butyrate degradation under methanogenic conditions.
authors.affiliation: Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Hatamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Imachi
authors.forename: Yuto
authors.initials: Y
authors.lastname: Yashiro
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Ohashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Harada
mesh_terms: D002087:Butyrates / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification*; D007553:Isotope Labeling; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D002087:Butyrates; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008697:Methane