Micromonospora chaiyaphumensis sp. nov., isolated from Thai soils.
Three actinomycete strains, MC5-1T, MC7-1 and R1-1, were isolated from soil samples collected in Thailand. Their taxonomic positions were determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus Micromonospora, i.e. meso-diaminopimelic acid and N-glycolyl muramic acid were present in the cell-wall peptidoglycan, the whole-cell sugars were of pattern D, the phospholipids were of type II and the cellular fatty acids were of type 3b. Phylogenetic analysis of the 16S rRNA gene sequences revealed a close relationship between strains MC5-1T, MC7-1 and R1-1 (99.8 % sequence similarity) and Micromonospora auratinigra JCM 12357T (99.3 %). The three novel strains were clearly distinguishable from M. auratinigra JCM 12357T from the low DNA-DNA relatedness (< or =43.4 %). On the basis of the data presented, strain MC5-1T represents a novel species of the genus Micromonospora, for which the name Micromonospora chaiyaphumensis is proposed. The type strain is MC5-1T (=KCTC 19332T=JCM 12873T=PCU 267T=TISTR 1564T).
Jongrungruangchok
Suchada
S
Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Tanasupawat
Somboon
S
Kudo
Takuji
T
D050260:Carbohydrate Metabolism; D005798:Genes, Bacterial; D008847:Micromonospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D009626:Terminology as Topic; D013785:Thailand
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S