title: Cationized hydroxyethylcellulose as a novel, adsorbed coating for basic protein separation by capillary electrophoresis.
abstract: We present cationized hydroxyethylcellulose (cat-HEC) synthesized in our laboratory as a novel physically adsorbed coating for CE. This capillary coating is simple and easy to obtain as it only requires flushing the capillary with polymer aqueous solution. A comparative study with and without polymers was performed. The adsorbed cat-HEC coating exhibited minimal interactions with basic proteins, providing efficient basic protein separations with excellent reproducibility. Under broad pHs, the amine groups are the main charged groups bringing about a global positive charge on the capillary wall. As a consequence, the cat-HEC coating produced an anodal EOF performance. A comparative study on the use of hydroxyethylcellulose (HEC) and cat-HEC as physically adsorbed coatings for CE are also presented. The separation efficiency and analysis reproducibility proved that the cat-HEC polymer was efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. The long-term stability of the cat-HEC coating in consecutive protein separation runs has demonstrated the suitability of the coating for high-throughput electrophoretic protein separations.
authors.affiliation: Joint Laboratory of Polymer Thin Films and Solution, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, PR China.
authors.forename: Runmiao
authors.initials: R
authors.lastname: Yang
authors.forename: Ronghua
authors.initials: R
authors.lastname: Shi
authors.forename: Shuhua
authors.initials: S
authors.lastname: Peng
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Hang
authors.initials: H
authors.lastname: Liu
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins; D002482:Cellulose; C002283:hydroxyethylcellulose