The role of alkylphosphocholines in retinal Müller glial cell proliferation.
OBJECTIVE
Alkylphosphocholines (APCs) are investigated for their effect on Müller glial cell proliferation and F-actin stress fiber distribution in vitro.


METHODS
Müller cells were incubated with APCs (C18:1-PC and C22:1-PC) +/- fetal calf serum. Proliferation was assessed by BrdU labeling and with the tetrazolium dye-reduction assay. Toxicity was measured using the trypan blue exclusion assay. The distribution of F-actin stress fibers was determined using FITC-phalloidin staining.


RESULTS
APCs are effective inhibitors of human and rat Müller glial cell proliferation and hypoxia-induced up-regulation of F-actin stress fibers in vitro in non-toxic concentrations.


CONCLUSIONS
APCs might prevent intraretinal changes as a result of serum stimulation and hypoxia following retinal detachment.
Eibl
Kirsten H
KH
University Eye Hospital, Ludwig-Maximilians-University, Munich, Germany. kirsten.eibl@med.uni-muenchen.de
Schwabe
Kerstin
K
Welge-Luessen
Ulrich
U
Kampik
Anselm
A
Eichler
Wolfram
W
D000199:Actins / Q000378:metabolism*; D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009994:Osmolar Concentration; D010767:Phosphorylcholine / Q000031:analogs & derivatives*; D051381:Rats; D020318:Rats, Long-Evans; D012160:Retina / Q000166:cytology*; D013194:Staining and Labeling; D022502:Stress Fibers / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D015854:Up-Regulation
D000199:Actins; D010767:Phosphorylcholine