title: Clonal population of flucytosine-resistant Candida tropicalis from blood cultures, Paris, France.
abstract: Candida tropicalis is a diploid ascomycetes yeast responsible for 4%-24% of candidemia. Resistance to flucytosine is rarely described for this species but was observed for 45 (35%) of 130 C. tropicalis isolates recovered from blood cultures in the Paris area in a 4-year survey. The aims of this study were to test the hypothesis that the flucytosine-resistant isolates could represent a subgroup and to determine the relationship between epidemiologic and genomic data. Epidemiologic data and gene sequences were analyzed, and molecular typing was performed. Our results suggest that a clone of flucytosine-resistant isolates, associated with malignancies and a lower mortality than that for other C. tropicalis isolates, is widespread in the Paris area. We propose the analysis of 2 polymorphic microsatellite markers coupled with URA3 sequencing to track the clone.
authors.affiliation: Institut Pasteur, Paris, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Desnos-Ollivier
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bretagne
authors.forename: Claire
authors.initials: C
authors.lastname: Bernède
authors.forename: Vincent
authors.initials: V
authors.lastname: Robert
authors.forename: Dorothée
authors.initials: D
authors.lastname: Raoux
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Chachaty
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Forget
authors.forename: Claire
authors.initials: C
authors.lastname: Lacroix
authors.forename: Françoise
authors.initials: F
authors.lastname: Dromer
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D041022:Candida tropicalis / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D002177:Candidiasis / Q000382:microbiology*; D025141:Drug Resistance, Fungal; D005437:Flucytosine / Q000494:pharmacology*; D005602:France; D016469:Fungemia / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D010641:Phenotype; D012307:Risk Factors; D013997:Time Factors
chemical_list: D000935:Antifungal Agents; D005437:Flucytosine