A rapid method for isolation of piperine from the fruits of Piper nigrum Linn.
A simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of Piper nigrum. The method involves extraction of the fruit powder with glacial acetic acid, from which piperine is partitioned into chloroform and subsequently crystallized. The identity of the compound was confirmed by its melting point, comparison of UV, IR, and mass spectral data with those from a reference standard, and co-chromatography with the reference standard using thin-layer chromatography (TLC). The purity of the compound was ascertained by TLC, by recording UV absorption spectra at the start, middle, and end positions of the spot on the plate, and by differential scanning calorimetry (DSC).
Kanaki
Niranjan
N
Department of Phytochemistry and Pharmacognosy, B. V. Patel Pharmaceutical Education and Research Development Centre, Thaltej-Gandhinagar highway, Thaltej, Ahmedabad, 380 054, Gujarat, India.
Dave
Mansi
M
Padh
Harish
H
Rajani
Mandapati
M
D000470:Alkaloids / Q000302:isolation & purification*; D052117:Benzodioxoles / Q000302:isolation & purification*; D002152:Calorimetry, Differential Scanning; D002855:Chromatography, Thin Layer; D005638:Fruit; D029222:Piper nigrum / Q000737:chemistry*; D010880:Piperidines / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D053284:Polyunsaturated Alkamides / Q000302:isolation & purification*; D012015:Reference Standards; D013057:Spectrum Analysis; D044366:Transition Temperature
D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; C008922:piperine