title: Cellular motility of Down syndrome gingival fibroblasts is susceptible to impairment by Porphyromonas gingivalis invasion.
abstract: BACKGROUND
  Severe periodontal breakdown is often associated with Down syndrome (DS); however, the etiology of this condition is not understood fully. Cellular motility of gingival fibroblasts is a critical event for wound healing and regeneration of periodontal tissues. Porphyromonas gingivalis is known to be a periodontal pathogen that invades host cells, contributing to periodontal destruction. In this study, we examined the influence of P. gingivalis infection on the motility of DS gingival fibroblasts (DGFs).
  
  
  METHODS
  DGFs and normal gingival fibroblasts (NGFs) were infected with P. gingivalis with type II fimbriae, and cellular motility was evaluated using an in vitro wounding assay. Protein degradation of alpha5beta1-integrin subunits and a migration-regulating signaling molecule, paxillin, were investigated using specific antibodies. The adhesion to and invasion of fibroblasts by P. gingivalis were determined with a colony forming assay. The gene expressions of alpha5beta1-integrin subunits were also quantified using a reverse transcription-polymerase chain reaction method.
  
  
  RESULTS
  The cellular motility of DGFs was impaired significantly by P. gingivalis compared to NGFs, and the former were invaded readily by P. gingivalis. Further, cellular paxillin from DGFs was degraded markedly by the pathogen. Although protein degradation of alpha5beta1 integrin was induced, its mRNA expression was not affected significantly.
  
  
  CONCLUSIONS
  P. gingivalis readily invades DGFs and subsequently degrades paxillin, which impairs cellular motility and likely prevents wound healing and the regeneration of periodontal tissues. These characteristics may be involved in the etiology of DS periodontitis.
authors.affiliation: Division of Special Care Dentistry, Osaka University Dental Hospital, Suita-Osaka, Japan.
authors.forename: Jumpei
authors.initials: J
authors.lastname: Murakami
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kato
authors.forename: Shinji
authors.initials: S
authors.lastname: Kawai
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Akiyama
authors.forename: Atsuo
authors.initials: A
authors.lastname: Amano
authors.forename: Ichijiro
authors.initials: I
authors.lastname: Morisaki
mesh_terms: D000293:Adolescent; D001422:Bacterial Adhesion / Q000502:physiology; D016022:Case-Control Studies; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004314:Down Syndrome / Q000473:pathology*; D005260:Female; D005347:Fibroblasts / Q000382:microbiology / Q000473:pathology*; D010861:Fimbriae, Bacterial / Q000145:classification; D005881:Gingiva / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D039081:Integrin alpha5beta1 / Q000032:analysis; D051419:Paxillin / Q000032:analysis; D016966:Porphyromonas gingivalis / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D039081:Integrin alpha5beta1; D051419:Paxillin