title: Downregulation of Tie2 gene by a novel antitumor sulfolipid, 3'-sulfoquinovosyl-1'-monoacylglycerol, targeting angiogenesis.
abstract: We previously reported that 3'-sulfoquinovosyl-1'-monoacylglycerol (SQMG) was effective in suppressing the growth of solid tumors due to hemorrhagic necrosis in vivo. In the present study, we investigated the antiangiogenic effect of SQMG. In vivo assessment of antitumor assays showed that some tumor cell lines, but not others, were sensitive to SQMG. Microscopic study suggested that in SQMG-sensitive tumors, but not SQMG-resistant tumors, angiogenesis was reduced. We next investigated gene expression relating to angiogenesis in tumor tissues by quantitative real-time polymerase chain reaction. Consequently, although vascular endothelial growth factor gene expression was not detected with significant differences among the cases, significant downregulation of Tie2 gene expression was observed in all SQMG-sensitive tumors as compared with controls, but not in SQMG-resistant tumors. These data suggested that the antitumor effects of SQMG could be attributed to antiangiogenic effects, possibly via the downregulation of Tie2 gene expression in SQMG-sensitive tumors.
authors.affiliation: Marine Biomedical Institute, Sapporo Medical University School of Medicine, S1, W17, Chuo-ku, Sapporo 060-8556, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mori
authors.forename: Hiroeki
authors.initials: H
authors.lastname: Sahara
authors.forename: Kayo
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ohta
authors.forename: Satoko
authors.initials: S
authors.lastname: Aoki
authors.forename: Masahiko
authors.initials: M
authors.lastname: Miura
authors.forename: Fumio
authors.initials: F
authors.lastname: Sugawara
authors.forename: Kengo
authors.initials: K
authors.lastname: Sakaguchi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Sato
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005260:Female; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000098:blood supply*; D009389:Neovascularization, Pathologic / Q000201:enzymology* / Q000235:genetics; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D042787:Receptor, TIE-2 / Q000235:genetics* / Q000378:metabolism
chemical_list: C477365:1-mono-O-acyl-3-O-(alpha-D-sulfoquinovosyl)-glyceride; D020533:Angiogenesis Inhibitors; D006017:Glycolipids; D047428:Protein Kinase Inhibitors; D042787:Receptor, TIE-2