Identification of functionally important amino acids in a novel indigo-producing oxygenase from Rhodococcus sp. strain T104.
A novel indigo-producing oxygenase gene, designated ipoA (1,197 bp) was characterized from Rhodococcus sp. strain T104. Three indigo-negative mutations (A58V, P59L, and G251D) were obtained through random mutagenesis using an E. coli mutator strain. Subsequent saturation mutagenesis resulted in the identification of nine and three amino acid substitutions that restore activity in the A58V and P59L mutants, respectively. Activity was not restored in the G251D mutation by any other amino acids. Interestingly, activity in the A58V mutant, where a methyl group is only replaced by an isopropyl side chain, is restored by a variety of amino acids, including polar ones. A molecular modeling study suggests that the residues at positions 58, 59, and 251 of the T104 IpoA enzyme are far from the active site, indicating that the mutations must alter the overall structure of the enzyme.
Kwon
Na Ra
NR
Department of Biology, Yonsei University, Seoul, Korea.
Chae
Jong-Chan
JC
Choi
Ki Young
KY
Yoo
Miyoun
M
Zylstra
Gerben J
GJ
Kim
Young Min
YM
Kang
Beom Sik
BS
Kim
Eungbin
E
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001665:Binding Sites; D007203:Indigo Carmine; D007211:Indoles / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010105:Oxygenases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012240:Rhodococcus / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment
D001426:Bacterial Proteins; D007211:Indoles; D007203:Indigo Carmine; D010105:Oxygenases