title: Separation of sanguinarine and chelerythrine in Macleaya cordata (Willd) R. Br. based on methyl acrylate-co-divinylbenzene macroporous adsorbents.
abstract: Sanguinarine (SAN) and chelerythrine (CHE) are known as major effective components in the quaternary benzo[c]phenanthridine isoquinoline alkaloids (QBA) fraction of Macleaya cordata (Willd) R. Br. but possess different biological activities. In this study, a method for the separation of SAN and CHE based on methyl acrylate-co-divinylbenzene (MA-co-DVB) macroporous adsorbents was established. The relationship between the polarities of the adsorbents and their adsorption-desorption behaviors towards SAN and CHE was investigated. The results showed that, among three selected commercial adsorbents and seven synthesized macroporous polymeric adsorbents with different MA content, the adsorbent No. 5 with 50% MA content provided the best separation power, and the two alkaloids were separated successfully in a gradient eluent process with 60% (v/v) ethanol aqueous and 80% ethanol aqueous contained 8% acetic acid. Dynamic adsorption and desorption tests had been performed in the column packed with the adsorbent No. 5 for optimizing the process parameters. Under the optimized conditions, the ratio of SAN and CHE transformed from 2:1 in the QBA fraction of M. cordata to 1:13 and 25:1 in the products obtained from the two-step gradient elution, and the recoveries of both SAN and CHE were nearly 90%.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China.
authors.forename: Guopei
authors.initials: G
authors.lastname: Pi
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.forename: Jiamei
authors.initials: J
authors.lastname: Yu
authors.forename: Rongfu
authors.initials: R
authors.lastname: Shi
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yuan
authors.forename: Chunhong
authors.initials: C
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D053119:Benzophenanthridines / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods*; D007546:Isoquinolines / Q000302:isolation & purification*; D028101:Papaveraceae / Q000737:chemistry*; D013343:Styrenes / Q000737:chemistry*
chemical_list: D053119:Benzophenanthridines; D007546:Isoquinolines; D013343:Styrenes; C530114:methyl acrylate-co-divinylbenzene; C005705:sanguinarine; C016299:chelerythrine