Gene expression profile of peripheral blood mononuclear cells in response to HIV-VLPs stimulation.
BACKGROUND
Baculovirus-expressed HIV-1 Pr55gag Virus-Like Particles (HIV-VLPs) induce maturation and activation of monocyte-derived dendritic cells (MDDCs) with a production of Th1- and Th2-specific cytokines.


RESULTS
The analysis of genomic transcriptional profile of MDDCs, obtained from normal healthy donors and activated by HIV-VLPs, show the modulation of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation. Similar data are obtained using peripheral blood mononuclear cells (PBMCs), without further selection, showing the feasibility of a direct and "simplified" experimental procedure.


CONCLUSIONS
The results here described show that the maturation pattern induced by HIV-VLPs in ex vivo generated MDDCs, can be observed also in CD14-expressing freshly derived PBMCs, with the possible identification of genetic predictors of individual response to immunogens.
Buonaguro
Luigi
L
Lab, Viral Oncogenesis and Immunotherapies & AIDS Reference Center, Department of Experimental Oncology, Istituto Nazionale Tumori "Fond, G, Pascale", 80131 Napoli, Italy. irccsvir@unina.it
Monaco
Alessandro
A
Aricò
Eleonora
E
Wang
Ena
E
Tornesello
Maria Lina
ML
Lewis
George K
GK
Marincola
Franco M
FM
Buonaguro
Franco M
FM
D000465:Algorithms; D001798:Blood Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D006678:HIV / Q000378:metabolism*; D054298:Human Immunodeficiency Virus Proteins / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism*; D014771:Virion / Q000378:metabolism*
D001798:Blood Proteins; D054298:Human Immunodeficiency Virus Proteins