title: [Effect of Ca2+ complexation on oxygen partial pressure in the Bladder Meridian in goats].
abstract: OBJECTIVE
  To explore the relationship between energy metabolism and meridian phenomenon. Methods Ten Boer hybridism goats mitigated under chlorpromazine (0.85 mg/kg, intramuscular injection) were subjected to the present study. Changes of oxygen partial pressure (PO2) in "Ganshu" (BL 18), "Dachangshu" (BL 25) and "Guanyuanshu" (BL 26) of Bladder Meridian and in 3 non-acupoints (3 cm apart from BL18, BL25 and BL26 separately on the unilateral side) were recorded by using a needle-type tissue oxygen tension sensor before and following local injection of EDTA-Na2 (200 microL, 0.05 kg/mol, for complexing Ca2+) and 0.9% saline.
  
  
  RESULTS
  (1) Compared with the corresponding non-acupoints, Ca2+ potentials in BL18, BL25 and BL26 were all significantly higher (P < 0.05, 0.01). (2) In comparison with the corresponding non-acupoints, the values of tissue PO2 of BL18 and BL26 were significantly higher (P < 0.01). (3) After injection of EDTA-Na2, PO2 values in both BL18, BL26, and their respective non-acupoints increased significantly in comparison with those of either injection of saline or their respective basal values (P < 0.01, 0.05). Compared with those of their corresponding non-acupoints PO2 values in both BL18 and BL26 increased markedly (P < 0.01).
  
  
  CONCLUSIONS
  Ca2+ potential of the Bladder Meridian was significantly higher than that of non-acupoints, and after complexing Ca2+, PO2 in acupoints and non-acupoints increased markedly, suggesting that Ca2+ may play an important role in meridian signaling and production of acupuncture effect.
authors.affiliation: Institute of Integrated Chinese and Western Medicine, Tongji Hospital, China. wangqixlp@hotmail.com
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Jiang
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Chen
authors.forename: Ming-min
authors.initials: MM
authors.lastname: Zhang
authors.forename: Ming-xing
authors.initials: MX
authors.lastname: Ding
authors.forename: E-sheng
authors.initials: ES
authors.lastname: Kong
authors.forename: Guang-ying
authors.initials: GY
authors.lastname: Huang
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D002118:Calcium / Q000502:physiology*; D004492:Edetic Acid / Q000494:pharmacology; D004594:Electrophysiology; D005260:Female; D006041:Goats / Q000502:physiology*; D016740:Meridians; D010100:Oxygen; D010313:Partial Pressure; D001743:Urinary Bladder
chemical_list: D004492:Edetic Acid; D010100:Oxygen; D002118:Calcium