Presence of cytosolic peroxiredoxin 2 in the erythrocyte membrane of patients with hereditary spherocytosis.
We studied 82 Portuguese individuals, 57 with hereditary spherocytosis (HS) and 25 unaffected controls. We performed standardized diagnosis tests, including electrophoretic membrane protein analysis to identify and quantify protein deficiencies underlying HS. Membrane bound hemoglobin (MBH) and band 3 profiles were determined as oxidative stress and aging markers. A protein of about 22 kDa, present in 21 of 57 HS patients, but not in controls, was identified as peroxiredoxin 2 (Prx2), by mass-spectroscopy and by immunoblotting. Human erythrocyte Prx2 is a peroxiredoxin with thiol-specific antioxidant activity. The presence of Prx2 in erythrocyte membranes was linked to higher levels of oxidative stress, as reflected by significantly increased MBH in those HS patients. No relation with HS clinical severity was observed and Prx2 was detected in all types of membrane protein abnormalities. Prx2 membrane linkage is associated with a higher oxidative stress susceptibility of HS erythrocytes.
Rocha
Susana
S
Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Porto, Portugal. susaroc@hotmail.com
Vitorino
Rui M P
RM
Lemos-Amado
Francisco M
FM
Castro
Elisabeth Bayer
EB
Rocha-Pereira
Petronila
P
Barbot
José
J
Cleto
Esmeralda
E
Ferreira
Fátima
F
Quintanilha
Alexandre
A
Belo
Luís
L
Santos-Silva
Alice
A
D004910:Erythrocyte Membrane / Q000737:chemistry*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000032:analysis*; D013103:Spherocytosis, Hereditary / Q000378:metabolism*
D006454:Hemoglobins; C086065:PRDX2 protein, human; D054464:Peroxiredoxins