title: Genotypic comparison of clinical Legionella isolates and patient-related environmental isolates in The Netherlands, 2002-2006.
abstract: This study investigated the hypothesis that the genotype distribution of Legionella isolates from sporadic patients with Legionnaires' disease differs from that of Legionella strains in the environment. An amplified fragment-length polymorphism (AFLP) assay was used to genotype patient-derived and environmental Legionella isolates. The three Legionella pneumophila genotypes isolated most frequently from human respiratory secretions were AFLP types 004 Lyon, 010 London and 006 Copenhagen. These genotypes were cultured significantly less frequently from environmental samples (50% vs. 4%; p <0.001). The most frequently observed L. pneumophila serogroup 1 genotype among patient-derived isolates was 004 Lyon (32%). This genotype was cultured from only one of 6458 environmental samples. The positive sample contained 1.26 x 10(6) CFU/mL and originated from a whirlpool spa that had not been disinfected and had been maintained at 36 degrees C for several months. Overall, the distribution of genotypes differed significantly among patient and environmental isolates. A possible explanation is that virulent strains may exist in potential environmental sources at undetectable concentrations.
authors.affiliation: Municipal Health Service Kennemerland, Haarlem, The Netherlands. jwdenboer@hdk.nl
authors.forename: J W
authors.initials: JW
authors.lastname: Den Boer
authors.forename: J P
authors.initials: JP
authors.lastname: Bruin
authors.forename: L P B
authors.initials: LP
authors.lastname: Verhoef
authors.forename: K
authors.initials: K
authors.lastname: Van der Zwaluw
authors.forename: R
authors.initials: R
authors.lastname: Jansen
authors.forename: E P F
authors.initials: EP
authors.lastname: Yzerman
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D015982:Bias; D005838:Genotype; D006801:Humans; D016952:Legionella pneumophila / Q000235:genetics* / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000453:epidemiology*; D009426:Netherlands / Q000453:epidemiology; D014871:Water Microbiology