Flow cytometric analysis of canine umbilical cord blood lymphocytes.
Lymphocyte subsets in canine umbilical cord blood were flow cytometrically analyzed and compared with those of the dams' peripheral blood. The proportion of CD3+ T lymphocytes, CD21+CD3- B lymphocytes, and CD3-CD21- non-T non-B lymphocytes in umbilical cord blood was 52.9%, 30.4%, and 16.7%, respectively. T lymphocyte/B lymphocyte ratio was significantly lower in the umbilical cord blood than in the dams' peripheral blood (2.1 +/- 1.4 versus 11.0 +/- 8.1, P < 0.001). In contrast, CD4+ lymphocyte/CD8+ lymphocyte ratio was significantly higher in the umbilical cord blood than in the dams' peripheral blood (7.6 +/- 2.2 versus 1.8 +/- 0.6, P<0.001). These findings clarified the phenotypic characters of canine umbilical cord blood lymphocytes.
Otani
Isao
I
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. otani@brs.nihon-u.ac.jp
Ohta
Kohei
K
Ishikawa
Akira
A
Yamada
Takeki
T
Ishinazaka
Tsuyoshi
T
Ohtaki
Tadatoshi
T
Tsumagari
Shigehisa
S
Kanayama
Kiichi
K
D000818:Animals; D016516:CD4-CD8 Ratio; D004285:Dogs / Q000097:blood* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D008214:Lymphocytes / Q000097:blood*