title: Contrast staining of Trichinella spiralis larvae in fresh, frozen and formalin preserved muscles.
abstract: Fresh, frozen-thawed and formalin-preserved muscle samples heavily infected with Trichinella larvae were cut into several pieces and stained with Giemsa and Leishman and the reference Haematoxylineosin (H & E) stain. Observation under microscope revealed that both muscle larvae and nurse cells in fresh and formalin preserved specimens appeared as purplish blue structures contrasting with the pinkish color of non-infected muscle fibers in both Giemsa and Leishman stains. These findings were confirmed in H & E stained samples. However, frozen samples did not show contrast stain.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Ain-Shams University, Cairo 11566, Egypt.
authors.forename: Nehad M
authors.initials: NM
authors.lastname: Ali
authors.forename: Hala S
authors.initials: HS
authors.lastname: Thabet
mesh_terms: D000818:Animals; D001399:Azure Stains; D005260:Female; D007814:Larva; D009132:Muscles / Q000469:parasitology*; D051381:Rats; D013048:Specimen Handling / Q000379:methods*; D017160:Trichinella spiralis / Q000302:isolation & purification* / Q000648:ultrastructure; D014235:Trichinellosis / Q000175:diagnosis / Q000469:parasitology
chemical_list: D001399:Azure Stains