[Frequent allelic losses in tumor-associated stromal cells and tumor epitelium of prostate cancer].
It has become increasingly clear that tumor microenvironment plays a critical role in carcinogenesis. Accumulation of genetic alterations is typical not only for cancer epithelial cells but tumor-associated fibroblasts as well. Tumor epithelia, tumor-associated stroma from prostatectomy specimens of patients with prostate cancer and cells from prostatic intraepithelial neoplasia (PIN) and adjacent stroma from males with PIN were isolated by using laser capture microdissection. Microsatellite allelotyping was evaluated using 4 highly polymorphic markers for chromosomal regions 8p22, 16q23-24 and 13q14. Incidences of alterations (loss of heterozygosity or allelic imbalance) were 48% for region 8p22, 72% for 16q23 and 37% for 13q14. The LOH frequencies in tumor-associated stroma cells were very similar. Alterations at chromosome 13q were significantly associated with advanced tumor stage, whereas AI at 16q was also associated with high Gleason score and lymph node metastasis. We find some incidences of allelic imbalance in premalignant lesions in epithelial (16-27%) and stromal (7-22%) components. Our results show that the frequencies of genetic aberrations are as high in stromal cells as in tumor cells.
Kekeeva
T V
TV
Popova
O P
OP
Shegaĭ
P V
PV
Zavalishina
L E
LE
Andreeva
Iu Iu
IuIu
Zaletaev
D V
DV
Nemtsova
M V
MV
D002877:Chromosomes, Human / Q000235:genetics*; D004847:Epithelial Cells / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D042822:Genomic Instability / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D019048:Prostatic Intraepithelial Neoplasia / Q000235:genetics* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D040641:Quantitative Trait Loci / Q000235:genetics; D017154:Stromal Cells / Q000473:pathology