Study of the biosynthesis of 1-octen-3-ol using a crude homogenate of Agaricus bisporus in a bioreactor.
1-Octen-3-ol and 10-oxo-trans-8-decenoic acid are metabolites of the breakdown of linoleic acid (LA) by mushroom enzymes. These compounds can be produced in a bioreactor using a crude mushroom homogenate and the exogenous addition of LA and oxygen. The factors' duration of blending, mushroom-buffer ratio, effect of a surfactant, whole against partially clarified reaction broths, purity of LA, and utilization of stumps instead of whole mushrooms were studied for their effect on reaction yield using a 1-L bioreactor. The results showed the feasibility of using the more inexpensive 60%-pure LA instead of the 99%-pure LA even when a yield loss was involved. Waste stumps could be used instead of whole mushrooms with a yield decline of 26%.
Morawicki
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RO
Department of Food Science, University of Arkansas, 2756 N. Young Ave., Fayetteville, AR 72704, USA. rmorawic@uark.edu
Beelman
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RB
D000364:Agaricus / Q000737:chemistry* / Q000201:enzymology; D019149:Bioreactors; D019787:Linoleic Acid / Q000378:metabolism*; D008041:Linoleic Acids / Q000032:analysis / Q000378:metabolism*; D000442:Octanols / Q000032:analysis / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology
C480517:10-oxo-8-decenoic acid; D008041:Linoleic Acids; D000442:Octanols; D013501:Surface-Active Agents; D019787:Linoleic Acid; C038844:1-octen-3-ol