Properties of an alginate-degrading Flavobacterium sp. strain LXA isolated from rotting algae from coastal China.
A novel bacterium exhibiting alginolytic activity was isolated from rotten algae. The alginate-degrading activity was detected in the culture supernatant by measuring the decrease in alginate viscosity or the increase in reducing sugars. Basic characterization showed that it was gram negative, rod shaped, yellow pigmented, and positive for oxidase and catalase, with a DNA G+C content of 35.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is related to members of the genus Flavobacterium. Sequence similarity values with their nearest phylogenetic neighbours ranged from 95.9% to 96.7%. Genotypic results, together with phenotypic characteristics, differentiated this species from related Flavobacterium organisms with validly published names, which suggests that the organism should be a new species of the genus Flavobacterium tentatively named as Flavobacterium sp. strain LXA.
An
Qing-Da
QD
Department of Chemical and Material Engineering, Dalian College of Light Industry, Dalian 116034, People's Republic of China.
Zhang
Gong-Liang
GL
Wu
Hai-Tao
HT
Zhang
Zhen-Cheng
ZC
Zheng
Guo-Shuang
GS
Zhang
Ying-Li
YL
Li
Xian-Zhen
XZ
Murata
Yoshiyuki
Y
D000464:Alginates / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D056890:Eukaryota / Q000378:metabolism / Q000382:microbiology*; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011133:Polysaccharide-Lyases / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000464:Alginates; D012336:RNA, Ribosomal, 16S; D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase