title: [Analysis of overexpression of vascular endothelial growth factor-C in patients with angioimmunoblastic T-cell lymphoma].
abstract: OBJECTIVE
  To explore the vascular endothelial growth factor-C (VEGF-C) expression and its clinical significance in malignant lymphoma.
  
  
  METHODS
  Lymphoma cells were isolated by laser microdissection. VEGF-C expression in lymphoma tissue and microdissected lymphoma cells was measured by realtime quantitative PCR. Meanwhile, vessel ultrastructure was identified by transmission electron microscopy.
  
  
  RESULTS
  Comparing with that in 8 patients with reactive lymphocyte hyperplasia, VEGF-C was overexpressed in angioimmunoblastic T-cell lymphoma, both in lymphoma tissue (n = 18, P = 0.0020) and in microdissected lymphoma cells (n = 10, P < 0.0001). Increased VEGF-C level was associated with bone marrow infiltration (P = 0.0039), skin involvement (P = 0.0046) and high-risk international prognostic index (P = 0.0302). In VEGF-C overexpressed cases, ultrastructural study showed dystrophic vessels, with swelling endothelial cells and absence of pericytes.
  
  
  CONCLUSIONS
  The value of VEGF-C expression might be a biomarker of disease progression in angioimmunoblastic T-cell lymphoma.
authors.affiliation: Department of Hematology, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Wei-li
authors.initials: WL
authors.lastname: Zhao
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Liu
authors.forename: François
authors.initials: F
authors.lastname: Plassa
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Jin
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Anne
authors.initials: A
authors.lastname: Janin
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Shen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000378:metabolism* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
chemical_list: D042582:Vascular Endothelial Growth Factor C