title: C677T polymorphism in the methylenetetrahydrofolate reductase gene is associated with primary closed angle glaucoma.
abstract: OBJECTIVE
  To determine whether or not there is an association of the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism with disease in cohorts of primary open-angle glaucoma (POAG) and primary closed-angle glaucoma (PCAG) from Pakistan.
  
  
  METHODS
  This was a prospective study consisting of 150 patients (90 POAG and 60 PCAG) and 70 control subjects. Genomic DNA was extracted from leukocytes of the peripheral blood. MTHFR C677T polymorphism analysis was performed by the polymerase chain reaction-restriction fragment length polymorphism (RFLP) technique.
  
  
  RESULTS
  The prevalence of the MTHFR C/T genotype was 22.2% in POAG, 13.3% in PACG, and 18.6% in controls whereas the MTHFR T/T genotype was present solely in the PACG group (6.9%). The difference regarding the T/T genotype between PACG and controls was statistically significant (p<0.01).
  
  
  CONCLUSIONS
  The MTHFR C677T polymorphism was found to be associated with PCAG but not POAG in patients of Pakistani origin.
authors.affiliation: Department of Biosciences, Comsats Institute of Information Technology, Islamabad, Pakistan.
authors.forename: Shazia
authors.initials: S
authors.lastname: Michael
authors.forename: Raheel
authors.initials: R
authors.lastname: Qamar
authors.forename: Farah
authors.initials: F
authors.lastname: Akhtar
authors.forename: Wajid Ali
authors.initials: WA
authors.lastname: Khan
authors.forename: Asifa
authors.initials: A
authors.lastname: Ahmed
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D003596:Cytosine; D005260:Female; D005838:Genotype; D015812:Glaucoma, Angle-Closure / Q000235:genetics*; D005902:Glaucoma, Open-Angle / Q000235:genetics; D006801:Humans; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D010154:Pakistan; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D013941:Thymine
chemical_list: D003596:Cytosine; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D013941:Thymine