Crystallization and preliminary X-ray crystallographic analysis of a carbonyl reductase from Candida parapsilosis.
A novel short-chain NADPH-dependent (S)-1-phenyl-1,2-ethanediol dehydrogenase (SCR) has been crystallized. Two distinct but related crystal forms of SCR were obtained using the hanging-drop vapour-diffusion method and a reservoir solution consisting of 18%(w/v) polyethylene glycol 2000 monomethyl ether and 8%(v/v) 2-propanol as the precipitant. The crystals were rhomboid in shape with average dimensions of 0.3 x 0.3 x 0.4 mm and diffracted to a resolution of 2.7-3.0 A. The crystal forms both belong to space group P2(1)2(1)2(1) and have unit-cell parameters a = 104.7, b = 142.8, c = 151.8 A and a = 101.1, b = 146.0, c = 159.8 A. The calculated values of V(M), rotation-function and translation-function solutions and consideration of potential crystal packing suggest that there are eight protein subunits per asymmetric unit.
Zhang
Rongzhen
R
Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
Xu
Yan
Y
Sun
Ying
Y
Nie
Yao
Y
Mu
Xiaoqing
X
Li
Xuemei
X
Zhang
Xuejun C
XC
Rao
Zhihe
Z
D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000737:chemistry*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D002175:Candida / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry*
D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase