Capillary electrophoresis of gene mutation.
This chapter illustrates the usefulness of capillary electrophoresis (CE) for the detection of gene mutation, i.e., point mutation, methylation, and microsatellite analysis. In order to provide a general description of the main results and challenges in the field, some relevant applications and reviews on CE of gene mutation are tabulated. Furthermore, some detailed experimental procedures are shown. Several CE methods of gene mutation detection were developed including the following: (1) single-strand conformation polymorphism with capillary electrophoresis; (2) SNaPshot analysis; (3) constant denaturant capillary electrophoresis; (4) microsatellite analysis; and (5) methylation analysis.
Xu
Guowang
G
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
Shi
Xianzhe
X
Zhao
Chunxia
C
Yuan
Kailong
K
Weng
Qianfeng
Q
Gao
Peng
P
Tian
Jing
J
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D019175:DNA Methylation; D019075:Electrophoresis, Capillary / Q000379:methods*; D005091:Exons / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D000070957:MutL Protein Homolog 1; D009154:Mutation / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D009691:Nucleic Acid Denaturation; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D011401:Promoter Regions, Genetic / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras); D013696:Temperature; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D018631:ras Proteins / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; C117307:KRAS protein, human; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; D000070957:MutL Protein Homolog 1; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins