AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles.
Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.
Hsiong
Susan X
SX
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.
Cooke
Peter H
PH
Kong
Hyun-Joon
HJ
Fishman
Marshall L
ML
Ericsson
Maria
M
Mooney
David J
DJ
D000464:Alginates / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry* / Q000648:ultrastructure; D006046:Gold / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission; D009842:Oligopeptides / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry
D000464:Alginates; D001672:Biocompatible Materials; D020100:Hydrogels; D009842:Oligopeptides; D001710:Biotin; D006046:Gold; C047981:arginyl-glycyl-aspartic acid; D019809:Streptavidin