title: A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity.
abstract: Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Wen-Kui
authors.initials: WK
authors.lastname: Yang
authors.forename: Xin-Yang
authors.initials: XY
authors.lastname: Li
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Lin
authors.forename: Yan-Ni
authors.initials: YN
authors.lastname: Lun
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Shao-Wu
authors.initials: SW
authors.lastname: Lv
authors.forename: Gang-Lin
authors.initials: GL
authors.lastname: Yan
authors.forename: Jun-Qiu
authors.initials: JQ
authors.lastname: Liu
authors.forename: Jia-Cong
authors.initials: JC
authors.lastname: Shen
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.forename: Gui-Min
authors.initials: GM
authors.lastname: Luo
mesh_terms: D000818:Animals; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000235:genetics*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000235:genetics; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics
chemical_list: D015801:Helminth Proteins; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase