title: Crystallization and preliminary X-ray crystallographic analysis of a carbonyl reductase from Candida parapsilosis.
abstract: A novel short-chain NADPH-dependent (S)-1-phenyl-1,2-ethanediol dehydrogenase (SCR) has been crystallized. Two distinct but related crystal forms of SCR were obtained using the hanging-drop vapour-diffusion method and a reservoir solution consisting of 18%(w/v) polyethylene glycol 2000 monomethyl ether and 8%(v/v) 2-propanol as the precipitant. The crystals were rhomboid in shape with average dimensions of 0.3 x 0.3 x 0.4 mm and diffracted to a resolution of 2.7-3.0 A. The crystal forms both belong to space group P2(1)2(1)2(1) and have unit-cell parameters a = 104.7, b = 142.8, c = 151.8 A and a = 101.1, b = 146.0, c = 159.8 A. The calculated values of V(M), rotation-function and translation-function solutions and consideration of potential crystal packing suggest that there are eight protein subunits per asymmetric unit.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China.
authors.forename: Rongzhen
authors.initials: R
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Yao
authors.initials: Y
authors.lastname: Nie
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Mu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.forename: Xuejun C
authors.initials: XC
authors.lastname: Zhang
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Rao
mesh_terms: D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000737:chemistry*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D002175:Candida / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase