Interleukin-10 containing normal human serum inhibits granzyme B release but not perforin release from alloreactive and EBV-specific T cell clones.
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor, is capable of inhibiting synthesis of pro-inflammatory cytokines like IFNgamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and T helper Type 1 cells. We observed that normal human serum, derived from a healthy individual but containing large amounts of IL-10, inhibited cytotoxic activity and interfered with granzyme B release from alloreactive cytotoxic T cell (CTL) clones in vitro, but did not affect perforin release. The addition of normal human serum containing high levels of anti-IL-10 IgG neutralized the inhibitory effects of IL-10 serum. Moreover, we have identified that cytotoxic activity and granzyme B release from an Epstein-Barr virus (EBV)-specific CTL clone was similarly inhibited in the presence of IL-10 serum, while perforin release was unaffected. Anti-IL-10 IgG serum also appeared to neutralize the inhibitory effect of IL-10 serum on an EBV-specific CTL clone.
Nishimura
Motoko
M
Department of Research and Development, Central Blood Institute, Japanese Red Cross Society, 2-1-67, Tatsumi, Koutou-ku, Tokyo 135-8521, Japan. m-nishimura@bs.jrc.or.jp
Sato
Hideya
H
Okazaki
Hitoshi
H
Satake
Masahiro
M
Tadokoro
Kenji
K
D017209:Apoptosis / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D002472:Cell Transformation, Viral / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D018920:Coculture Techniques; D003601:Cytotoxicity Tests, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D053804:Granzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000097:blood / Q000494:pharmacology*; D054353:Perforin / Q000378:metabolism*; D044967:Serum / Q000737:chemistry / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism
D018984:Epitopes, T-Lymphocyte; D007074:Immunoglobulin G; D054353:Perforin; D016753:Interleukin-10; D053804:Granzymes