Genotoxic effects and LC50 value of NaOCl on Orthrias angorae (Steindachner 1897).
Studies show that different organisms used as bio-indicators have indicated several genotoxic and mutagenic effects of disinfected waters. In this study, the 96 h LC(50 )mean value of NaOCl for Orthrias angorae was calculated to be 0.5509 mg/L. The results showed that NaOCl is highly toxic to O. angorae specimens. Statistical analysis demonstrated a significant increase in micronuclei after the induction of 0.5 mg/L NaOCl concentration after 36 h. The same increase has been reported for 0.37 and 0.5 mg/L NaOCl concentrations after 72 h. Even though the MN frequency of 0.37 mg/L was similar after 36 and 72 h, only 72 h micronuclei frequency was statistically significant. The 72 h MN frequency of the negative control group was smaller than 36 h MN frequency of the negative control group. This discrepancy has led to 72 h MN frequency being statistically significant. MN frequency of 0.25 mg/L NaOCl concentration was insignificant when compared to negative test groups. The benzene treatment also caused a significant increase (p < 0.01) in the frequency of micronucleated erythrocytes.
Gül
Süleyman
S
Department of Biology, Faculty of Sciences and Arts, University of Kafkas, Kars, Turkey. suleymangul@hotmail.com
Ozkan
Oktay
O
Nur
Gökhan
G
Aksu
Pinar
P
D000818:Animals; D004202:Disinfectants / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D005399:Fishes; D007928:Lethal Dose 50; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D012973:Sodium Hypochlorite / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D004202:Disinfectants; D009153:Mutagens; D014874:Water Pollutants, Chemical; D012973:Sodium Hypochlorite