Molecular analysis of drug-resistant Mycobacterium tuberculosis isolates collected in central Poland.
In total, 48 Mycobacterium tuberculosis clinical isolates from 48 patients with drug-resistant pulmonary tuberculosis (TB) in central Poland during 2004 were analysed by spoligotyping and IS6110-Mtb1/Mtb2 PCR. Twelve (25%) isolates were clustered by spoligotyping in combination with IS6110-Mtb1/Mtb2 PCR. Patients from whom these isolates were obtained were assumed to have developed TB as a result of recent transmission. Spoligotyping, used alone, overestimated the number of clustered isolates. However, combined use of spoligotyping and IS6110-Mtb1/Mtb2 PCR was an efficient approach for revealing clonal relatedness among M. tuberculosis isolates.
Augustynowicz-Kopeć
E
E
Department of Microbiology, National Tuberculosis and Lung Diseases Research Institute, Warsaw, Poland.
Jagielski
T
T
Kozińska
M
M
Zabost
A
A
Klatt
M
M
Zwolska
Z
Z
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D010641:Phenotype; D011044:Poland / Q000453:epidemiology; D015995:Prevalence; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology*
D004269:DNA, Bacterial