Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone.
Benzalacetone synthase (BAS) from Rheum palmatum is a plant-specific type III polyketide synthase that catalyzes the one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group P2(1), with unit-cell parameters a = 54.6, b = 89.6, c = 81.1 A, alpha = gamma = 90.0, beta = 100.5 degrees . Diffraction data were collected to 1.8 A resolution using synchrotron radiation at BL24XU of SPring-8.
Morita
Hiroyuki
H
Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
Tanio
Michikazu
M
Kondo
Shin
S
Kato
Ryohei
R
Wanibuchi
Kiyofumi
K
Noguchi
Hiroshi
H
Sugio
Shigetoshi
S
Abe
Ikuro
I
Kohno
Toshiyuki
T
D000217:Acyltransferases / Q000737:chemistry* / Q000378:metabolism; D002074:Butanones / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000737:chemistry
D002074:Butanones; D010940:Plant Proteins; D011994:Recombinant Proteins; C025383:benzylideneacetone; D000217:Acyltransferases; C020141:flavanone synthetase