Isolation and identification of antioxidants from Pedilanthus tithymaloides.
A bioassay-guided methodology utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH), the trolox equivalent antioxidant capacity (TEAC), and reducing power assays, as well as an assessment of scavenging properties against O (2) (.-) , H(2)O(2), HOCl, ROO., .NO, and ONOO(-) were used to find the main antioxidant principles of Pedilanthus tithymaloides (Euphorbiaceae), a shrub used in traditional Cuban medicine. The principles were identified as kaempferol 3-O-beta-D-glucopyranoside-6''-(3-hydroxy-3-methylglutarate), quercitrin, isoquercitrin, and scopoletin. The contents of total phenolics and flavonoids were found to be 76.0 +/- 4.8 mg of gallic acid equivalents/g extract and 9.8 +/- 0.4 mg of rutin equivalents/g extract, respectively.
Abreu
Pedro M
PM
Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, CQFB/REQUIMTE, 2829-516, Caparica, Portugal. pma@dq.fct.unl.pt
Matthew
Susan
S
González
Tania
T
Vanickova
Lucie
L
Costa
David
D
Gomes
Ana
A
Segundo
Marcela A
MA
Fernandes
Eduarda
E
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001681:Biological Assay; D005591:Chemical Fractionation / Q000379:methods; D019665:Euphorbiaceae / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D005960:Glucosides / Q000302:isolation & purification; D044949:Kaempferols / Q000302:isolation & purification; D015394:Molecular Structure; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification; D012603:Scopoletin / Q000302:isolation & purification
D000975:Antioxidants; D005609:Free Radicals; D005960:Glucosides; D044949:Kaempferols; C090063:kaempferol 3-O-glucopyranoside-6''-(3-hydroxy-3-methyl glutarate); C016527:isoquercitrin; C012526:quercitrin; D011794:Quercetin; D012603:Scopoletin