title: Oestrogen and progesterone receptor binding capacity and oestrogen receptor alpha expression (ERalpha mRNA) along the cervix of cycling ewes.
abstract: The aim of the present work was to study the oestrogen receptor (ER) and progesterone receptor (PR) binding capacity and the oestrogen receptor alpha (ERalpha) mRNA concentration in cranial and caudal cervix during the ovine oestrous cycle. Cervical samples of synchronised Corriedale ewes were obtained on Day 1 (n=7), 6 (n=6) or 13 (n=7) after oestrus detection (Day 0). The ER and PR binding capacity by ligand-binding assay and the ERalpha mRNA concentration by solution hybridisation in both cranial and caudal zones of the cervix were determined. The ER and PR binding capacity were higher (P<0.005) on Day 1 than on Days 6 and 13 in both cranial and caudal zones. The ERalpha mRNA concentrations were higher (P<0.0001) on Day 1 than on Days 6 and 13 only in the caudal zone. The PR binding capacity and ERalpha mRNA concentration were higher (P<0.005) in the caudal than in the cranial zone on Day 1. The ER and PR expression in the ovine cervix varied during the oestrous cycle in agreement with the known upregulation exerted by oestrogen and downregulation exerted by progesterone. Differences in ER and PR expression along the longitudinal axis of the ovine cervix were found, reflecting histological and functional differences between the cranial and caudal zones.
authors.affiliation: Biochemistry, Department of Molecular and Cellular Biology, Faculty of Veterinary, Montevideo, Uruguay. marodri@adinet.com.uy
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Piñón
authors.forename: C
authors.initials: C
authors.lastname: Tasende
authors.forename: P
authors.initials: P
authors.lastname: Puime
authors.forename: E G
authors.initials: EG
authors.lastname: Garófalo
mesh_terms: D000818:Animals; D002584:Cervix Uteri / Q000378:metabolism*; D015536:Down-Regulation; D047628:Estrogen Receptor alpha / Q000096:biosynthesis; D030762:Estrous Cycle / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D012756:Sheep; D015854:Up-Regulation
chemical_list: D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone