Suspended-drop electroporation for high-throughput delivery of biomolecules into cells.
We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.
Guignet
Emmanuel G
EG
Department of Chemical and Systems Biology, Bio-X Program, Clark Center, 318 Campus Drive, Stanford University School of Medicine, Stanford, California 94305, USA. guignet@stanford.edu
Meyer
Tobias
T
D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D003911:Dextrans / Q000378:metabolism; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D020868:Gene Silencing; D006801:Humans; D009474:Neurons / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
D018076:DNA, Complementary; D003911:Dextrans; D034741:RNA, Small Interfering