title: P53 and BCL-2 as prognostic markers in endometrial carcinoma.
abstract: The objective of this study was to verify the frequency of p53 and BCL-2 immunohistochemical expression in patients with endometrial carcinoma and to correlate it with histological factors (histological type, tumor grade, depth of myometrial invasion, lymph node involvement and surgical staging) and survival. Forty-eight patients with endometrial carcinoma who were submitted to primary surgical treatment were assessed. p53 and BCL-2 immunohistochemical expression was determined using paraffin blocks containing the tumor area. p53 and BCL-2 expression was detected in 39.6% and 58.3% of the tumors, respectively. No significant difference was found regarding the frequency of p53 expression when analyzing histological type (33.3% in endometrioid tumors, 58.3% in non-endometrioid tumors; p = 0.176), depth of myometrial invasion (p = 0.632) and surgical staging (I-11.1%, II-66.7%, III-57.1%; p = 0.061). p53 expression was significantly more frequent in undifferentiated tumors (p = 0.007) and in those showing lymph node involvement (p = 0.030). Univariate analysis showed a positive association with death (RR, 3.358; CI, 1.386-8.134; p = 0.005) and short-term survival. The present study did not reveal any correlation between BCL-2 expression and histopathologic markers or survival. In conclusion, this study showed that p53 expression is directly correlated with undifferentiated tumors, lymph-node involvement and risk of death. On the other hand, BCL-2 expression was not correlated with any known histological factors.
authors.affiliation: Department of Gynecology and Obstetrics, Hospital de Clínicas de Porto Alegre, School of Medicine, Federal University of Rio Grande do Sul, Rua Comendador Caminha, Porto Alegre, Rio Grande do Sul, Brazil. marciaappel@terra.com.br
authors.forename: Márcia L M
authors.initials: ML
authors.lastname: Appel
authors.forename: Maria I
authors.initials: MI
authors.lastname: Edelweiss
authors.forename: James
authors.initials: J
authors.lastname: Fleck
authors.forename: Luis F
authors.initials: LF
authors.lastname: Rivero
authors.forename: Waldemar A
authors.initials: WA
authors.lastname: Rivoire
authors.forename: Heleusa I
authors.initials: HI
authors.lastname: Mônego
authors.forename: Ricardo
authors.initials: R
authors.lastname: Dos Reis
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis*; D018269:Carcinoma, Endometrioid / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D016009:Chi-Square Distribution; D016889:Endometrial Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53