A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase.
To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
Kwon
Yeong-Deok
YD
Department of Life Science, Catholic University of Korea, Bucheon, Gyunggi 420-743, Korea.
Lee
Sang Yup
SY
Kim
Pil
P
D000255:Adenosine Triphosphate / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D020869:Gene Expression Profiling; D005943:Gluconeogenesis / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000235:genetics* / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D000255:Adenosine Triphosphate; D010730:Phosphoenolpyruvate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP)