Osteoclasts support the survival of human plasma cells in vitro.
The aim of this in vitro study was to evaluate if osteoclasts (OCs) and dendritic cells (DCs), both of monocyte origin, can support the survival of normal human plasma cells (PCs). PCs differentiate from plasmablasts (PBs) arising from activated B cells, essentially memory B cells. To study the survival of both PBs (CD20(low)CD38(high)CD138(neg)) and PCs (CD20(neg)CD38(bright)CD138(bright)), we generated pre-PBs (CD20(low)CD38(pos)CD138(neg)) from CD40-activated B cells (CD20(high)CD38(neg)CD138(neg)) and cultured them on DCs or OCs in the presence of added IL-6. By quantitative and qualitative study, we showed that DCs support the survival of PBs and early PCs, but not that of PCs. In contrast, OCs support the survival of PBs, early PCs and PCs. PCs surviving on OCs 12 days after pre-PB input display phenotypic features of bone marrow PCs, CD138(bright)CD38(bright)HLA-DR(low)CD45(dim). The ability for OCs to support the survival of PCs was fully dependent on cell-cell contact and not inhibited by BCMA-Fc suggesting that secreted BAFF and APRIL were not involved.
Geffroy-Luseau
Alexandrine
A
INSERM U601, Department of Cancer Research, Biology Institute, 9 Quai Moncousu, F-44000 Nantes, France.
Jégo
Gaëtan
G
Bataille
Régis
R
Campion
Loïc
L
Pellat-Deceunynck
Catherine
C
D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation / Q000276:immunology; D002469:Cell Separation; D002470:Cell Survival / Q000276:immunology; D018920:Coculture Techniques; D003260:Contact Inhibition / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation; D008213:Lymphocyte Activation; D010010:Osteoclasts / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
D015703:Antigens, CD; D019013:CD40 Antigens