Application of ion trap technology to liquid chromatography/mass spectrometry quantitation of large peptides.
Triple quadrupole mass spectrometers are generally considered the instrument of choice for quantitative analysis. However, for the analysis of large peptides we have encountered some cases where, as the data presented here would indicate, ion trap mass spectrometers may be a good alternative. In general, specificity and sensitivity in bioanalytical liquid chromatography/mass spectrometry (LC/MS) assays are achieved via tandem MS (MS/MS) utilizing collision-induced dissociation (CID) while monitoring unique precursor to product ion transitions (i.e. selected reaction monitoring, SRM). Due to the difference in CID processes, triple quadrupoles and ion traps often generate significantly different fragmentation spectra of product ion species and intensities. The large peptidic analytes investigated here generated fewer fragments with higher relative abundance on the ion trap as compared to those generated on the triple quadrupole, resulting in lower limits of detection on the ion trap.
Shipkova
Petia
P
Bristol-Myers Squibb Company, PO Box 5400, Princeton, NJ 08543, USA. petia.shipkova@bms.com
Drexler
Dieter M
DM
Langish
Robert
R
Smalley
James
J
Salyan
Mary Ellen
ME
Sanders
Mark
M
D002138:Calibration; D002853:Chromatography, Liquid; D004867:Equipment Design; D052216:Glucagon-Like Peptide 1 / Q000737:chemistry; D007202:Indicators and Reagents; D036341:Intercellular Signaling Peptides and Proteins; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D012015:Reference Standards; D014667:Vasopressins / Q000737:chemistry
D007202:Indicators and Reagents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014667:Vasopressins; D052216:Glucagon-Like Peptide 1; C043046:helospectin I