title: Analysis of acetylcholine, choline and butyrobetaine in human liver tissues by hydrophilic interaction liquid chromatography-tandem mass spectrometry.
abstract: The strong polar quaternary ammoniums, acetylcholine (ACh), choline (Ch) and butyrobetaine (BB, (3-carboxypropyl)trimethylammonium), are believed playing important roles in liver metabolism. These metabolites are at low levels and are weakly retained on reversed-phase liquid chromatographic (RP-LC) columns. Several hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) methods have been reported to analyze these compounds from different samples. However, no application to human liver tissues has been published. In this study, HILIC-MS/MS method was developed to simultaneously determine these three metabolites in human liver tissues. They were simply extracted from tissue, separated on a HILIC column, and detected by tandem MS in the mode of multiple reaction monitoring (MRM). Further studies on the recovery and repeatability based on real samples indicated the method was accurate and reliable. This method was successfully applied to measure the levels of ACh, Ch and BB in 61 human liver tissue samples including normal, hepatocellular carcinoma (HCC) and matched non-cancerous liver tissues. By comparison of Ch and ACh contents in 29 HCC with their matched non-cancerous liver tissues, it was found that ACh content increased in 11/29 HCC cases and decreased in 13/29 cases. Furthermore, the ACh/Ch ratio increased in 16/29 HCC cases, while it decreased in 8/29 cases. These results strongly indicated that there exist different patterns of ACh content in cancer tissues among HCC patients, thus highlighting the understanding of ACh and its relevant signal pathways in hepatic carcinogenesis and HCC progression.
authors.affiliation: National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Xianzhe
authors.initials: X
authors.lastname: Shi
authors.forename: Dafang
authors.initials: D
authors.lastname: Wan
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.forename: Xianghuo
authors.initials: X
authors.lastname: He
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Shengli
authors.initials: S
authors.lastname: Yang
authors.forename: Jianren
authors.initials: J
authors.lastname: Gu
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D000109:Acetylcholine / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D001622:Betaine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D006528:Carcinoma, Hepatocellular / Q000737:chemistry; D002331:Carnitine / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002794:Choline / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D008113:Liver Neoplasms / Q000737:chemistry; D015394:Molecular Structure; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001622:Betaine; C002889:gamma-butyrobetaine; D002794:Choline; D000109:Acetylcholine; D002331:Carnitine