title: Pregnancy outcome after preimplantation genetic diagnosis in an affected couple with X-linked adrenoleukodystrophy.
abstract: OBJECTIVE
  To achieve a pregnancy free of X-linked adrenoleukodystrophy (X-ALD) by intracytoplasmic sperm injection (ICSI) and preimplantation genetic diagnosis (PGD).
  
  
  METHODS
  Case report.
  
  
  METHODS
  Clínica FIV Recoletos, a private IVF center.
  
  
  METHODS
  A couple in which the man had X-ALD.
  
  
  METHODS
  The ICSI protocol and PGD of the obtained embryos.
  
  
  METHODS
  Blastomeres were analyzed by fluorescence in situ hybridization using sex selection techniques. Embryos were transferred and pregnancy was diagnosed by hCG analysis and ultrasonographic examination.
  
  
  RESULTS
  Ten embryos were obtained by ICSI. A biopsy was taken from eight embryos to perform PGD and two male embryos were transferred resulting in a twin pregnancy.
  
  
  CONCLUSIONS
  This is the first registered gestation in which PGD has been used to prevent X-ALD transmission.
authors.affiliation: Department of In Vitro Fertilization, Clínica FIV Recoletos, Ciudad Real, Spain. miriamen1@yahoo.es
authors.forename: Miriam
authors.initials: M
authors.lastname: Iglesias
authors.forename: Patricia
authors.initials: P
authors.lastname: Ceballos
authors.forename: Carles
authors.initials: C
authors.lastname: Giménez
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: García-Nebreda
authors.forename: Raquel
authors.initials: R
authors.lastname: Domínguez
authors.forename: Alberto
authors.initials: A
authors.lastname: García-Enguídanos
mesh_terms: D000326:Adrenoleukodystrophy / Q000175:diagnosis* / Q000235:genetics; D000328:Adult; D002887:Chromosomes, Human, Pair 18; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D004624:Embryo Transfer; D005260:Female; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011247:Pregnancy; D011272:Pregnancy, Multiple; D019836:Preimplantation Diagnosis; D012732:Sex Determination Analysis; D012743:Sex Preselection; D020554:Sperm Injections, Intracytoplasmic; D014427:Twins; D055815:Young Adult