Mapping the binding site of thiopeptide antibiotics by proximity-induced covalent capture.
Proximity-induced covalent capture (PICC) has been established for the investigation of ligand binding to composite protein/oligonucleotide target complexes. The RNA-induced attachment of the thiopeptides Thiostrepton and Nosiheptide to engineered Cys mutants of the ribosomal protein L11 was highly position selective and allowed mapping of their binding site at amino acid resolution.
Baumann
Sascha
S
Fakultät Chemie, Technische Universität Dortmund, Otto-Hahn-Str. 6, D-44221 Dortmund, Germany.
Schoof
Sebastian
S
Harkal
Surendra D
SD
Arndt
Hans-Dieter
HD
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D003545:Cysteine / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009154:Mutation; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D008024:Ligands; D009841:Oligonucleotides; D010455:Peptides; D012269:Ribosomal Proteins; D013438:Sulfhydryl Compounds; C027624:ribosomal protein L11; D003545:Cysteine