Possible involvement of prolactin in the synthesis of lactoferrin in bovine mammary epithelial cells.
Bovine mammary epithelial cells (bMECs) synthesize lactoferrin, which is secreted into milk. Our results suggest that prolactin stimulated secretion of lactoferrin in primary bMECs and their clonal cell line under serum-free conditions. Prolactin also stimulated mRNA expression of lactoferrin in the clonal cell line. This effect was reduced by AG-490, suggesting that the prolactin-stimulated mRNA expression of lactoferrin was mediated by Janus kinase (JAK)2.
Nakajima
Kei-ichi
K
National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization, 1 Hitsujigaoka, Toyohira-Ku, Sapporo 062-8555, Japan. k1nakaji@affrc.go.jp
Nakamura
Masato
M
Gao
Xiao-Dong
XD
Kozakai
Takaharu
T
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002999:Clone Cells; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D053612:Janus Kinases / Q000378:metabolism; D007781:Lactoferrin / Q000096:biosynthesis*; D008321:Mammary Glands, Animal / Q000166:cytology*; D011388:Prolactin / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology
D012333:RNA, Messenger; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D011388:Prolactin; D053612:Janus Kinases; D007781:Lactoferrin