title: Proteomics of two cultivated mushrooms Sparassis crispa and Hericium erinaceum provides insight into their numerous functional protein components and diversity.
abstract: Mushroom can be defined as a macrofungus with a distinctive fruiting body. Mushrooms of class Basidiomycete are primarily wood degradation fungi, but serve as food and a part of traditional medicine used by humans. Although their life cycle is fairly well-established, the information on the molecular components, especially proteins are very limited. Here, we report proteomics analysis of two edible mushrooms (fruiting bodies) Sparassis crispa and Hericium erinaceum using one- and two-dimensional gel electrophoresis (1-DGE and 2-DGE) based complementary proteomics approaches. 1-DGE coupled with liquid chromatography and mass spectrometry identified 77 (60 nonredundant proteins) and 121 (88 nonredundant proteins) proteins from S. crispa and H. erinaceum, respectively. 2-DGE analysis revealed 480 and 570 protein spots stained with colloidal coomassie brilliant blue in S. crispa and H. erinaceum, respectively. Of the 71 and 115 selected protein spots from S. crispa and H. erinaceum 2D gel blots on polyvinyldifluoride (PVDF) membranes, respectively, 29 and 35 nonredundant proteins were identified by N-terminal amino acid sequencing. Identified nonredundant proteins from 1- or 2-DGE belonged to 19 functional categories. Twenty-one proteins were found common in both S. crispa and H. erinaceum proteomes, including 14-3-3 protein and septin. Together this study provides evidence for the presence of a large number of functionally diverse proteins, expressed in the fruiting body of two economically important mushrooms, S. crispa and H. erinaceum. Data obtained from 1-DGE and 2-DGE analyses is accessible through the mushroom proteomics portal http://foodfunc.agr.ibaraki.ac.jp/mushprot.html.
authors.affiliation: Food Function Laboratory, School of Agriculture, Ibaraki University, Ami 300-0393, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Horie
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Misato
authors.initials: M
authors.lastname: Hirano
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.forename: Hyung Wook
authors.initials: HW
authors.lastname: Nam
authors.forename: Yu Sam
authors.initials: YS
authors.lastname: Kim
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kouzuma
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masuo
authors.forename: Masami
authors.initials: M
authors.lastname: Yonekura
mesh_terms: D000363:Agaricales / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis* / Q000145:classification / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis / Q000235:genetics; D040901:Proteomics / Q000379:methods*
chemical_list: D005656:Fungal Proteins; D020543:Proteome