Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis, or to a multiplexing combination.
Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them as having excellent potential as donors for FRET and, in principle, differently colored QDs could be excited simultaneously. The present work describes the preparation and operation of FRET-based QD bioprobes individually able to detect the actions of protease, deoxyribonuclease, DNA polymerase, or changes in pH. In addition, two such QD-mounted biosensors were excited at a single wavelength, and shown to operate simultaneously and independently of each other in the same sample solution, allowing multiplex detection of the action of a protease, trypsin, in the presence of deoxyribonuclease.
Suzuki
Miho
M
Functional Materials Science, Graduate school of Science and Engineering, Saitama University, 225, Shimo-okubo, Sakura-ku, Saitama, Saitama, 338-8570, Japan. miho@fms.saitama-u.ac.jp
Husimi
Yuzuru
Y
Komatsu
Hirokazu
H
Suzuki
Koji
K
Douglas
Kenneth T
KT
D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000378:metabolism; D003851:Deoxyribonucleases / Q000032:analysis / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism; D045663:Quantum Dots; D012996:Solutions / Q000737:chemistry; D014357:Trypsin / Q000032:analysis / Q000378:metabolism
D012996:Solutions; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D010447:Peptide Hydrolases; D014357:Trypsin