title: [Expression of brain natriuretic peptide and c-fos gene in rat after acute myocardial ischemia and its medicolegal significance].
abstract: OBJECTIVE
  To study the mRNA expression of BNP and c-fos gene in rat heart after acute myocardial ischemia (AMI) and to provide a marker for its medicolegal diagnosis.
  
  
  METHODS
  AMI animal model of rat was made by ligating LAD. mRNA expression of BNP and c-fos gene were studied with RT-qPCR and ordinary PCR at 10 min, 30 min, 60 min and 3h after the successful ligation. The H&E staining was also used. Changes of the mRNA expression in different time groups were compared.
  
  
  RESULTS
  There was significant difference in BNP mRNA expression of the 3 h group by RT-qPCR compared with normal control group, 10 min, 30 min, and 60 min groups (P < 0.05). There were dramatic differences in c-fos mRNA expression between every two groups (P < 0.05) except between the normal group and the 10 min group, between the 30 min group and the 3 h group. The peak of c-fos expression was in 60 min group. No difference was shown between groups by the ordinary PCR. Myocardial fiber acidophilia staining and wavy changes could be seen occasionally at 3 h experimental group by H&E staining.
  
  
  CONCLUSIONS
  C-fos gene probably be used as an auxiliary test for myocardial ischemia of duration of 30 minutes or longer. RT-qPCR may be suitable for diagnosis of early AMI.
authors.affiliation: Department of Forensic Science, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. xyc1123@126.com
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Xu
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Jing
authors.forename: Yong'
authors.initials: Y
authors.lastname: Ke
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D005554:Forensic Medicine; D008297:Male; D017202:Myocardial Ischemia / Q000235:genetics / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D015415:Biomarkers; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain