title: Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals.
abstract: We describe a protocol for imaging bacterial luciferase (Lux)-expressing bacteria in small living animals. In this protocol, light emitted by Lux-expressing bacteria is detected and monitored by a cooled charge-coupled device detector. When these bacteria are administered to animals, it provides a potentially valuable approach to generate sensitive whole-body images with extremely low background. This imaging technology should enable the real-time monitoring of bacterial migration into both primary and metastatic tumors in several different mouse tumor models at a strong quantification power.
authors.affiliation: Laboratory of In Vivo Molecular Imaging, Department of Nuclear Medicine, Chonnam National University Medical School, 5 Hak1 dong, Dong-gu, Gwangju 501-746, Republic of Korea. jjmin@jnu.ac.kr
authors.forename: Jung-Joon
authors.initials: JJ
authors.lastname: Min
authors.forename: Vu H
authors.initials: VH
authors.lastname: Nguyen
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yeongjin
authors.initials: Y
authors.lastname: Hong
authors.forename: Hyon E
authors.initials: HE
authors.lastname: Choy
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D005456:Fluorescent Dyes; D005818:Genetic Engineering; D049449:Luminescence; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes