Expression of alpha-actinin-1 in human glomerular mesangial cells in vivo and in vitro.
Recent studies have demonstrated important roles of alpha-actinins in glomerular disease, while little information is known about the expression profile of alpha-actinins in human glomerular mesangial cells. Here, immunofluorescence and confocal microscopy showed that alpha-actinin-1 exclusively distributed along mesangial cells in human glomeruli of IgA nephropathy. RT-PCR and Western blot further confirmed the expression of alpha-actinin-1 in primary cultured human mesangial cells. We also found that transforming growth factor-beta 1 (TGF-beta 1) stimulated ACTN1 gene transcription and that transiently transfected alpha-actinin-1 significantly increased TGF-beta 1-induced plasminogen activator inhibitor-1 (PAI-1) promoter activity in human mesangial cells. These findings suggest that alpha-actinin-1 may play a role in human glomerular disease.
Yang
Chen
C
8701 Watertown Plank Road, Milwaukee, WI 53226, USA. chenyang@mcw.edu
Glass
William F
WF
D000185:Actinin / Q000096:biosynthesis*; D001706:Biopsy; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005922:Glomerulonephritis, IGA / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D050527:Mesangial Cells / Q000378:metabolism* / Q000473:pathology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis; D011379:Prognosis; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D017395:Plasminogen Activator Inhibitor 1; D053773:Transforming Growth Factor beta1; D000185:Actinin