title: A simple and inexpensive on-column frit fabrication method for fused-silica capillaries for increased capacity and versatility in LC-MS/MS applications.
abstract: A modified sol-gel method for a one-step on-column frit preparation for fused-silica capillaries and its utility for peptide separation in LC-MS/MS is described. This method is inexpensive, reproducible, and does not require specialized equipments. Because the frit fabrication process does not damage polyimide coating, the frit-fabricated column can be tightly connected on-line for high pressure LC. These columns can replace any capillary liquid transfer tubing without any specialized connections up-stream of a spray tip column. Therefore multiple columns with different phases can be connected in series for one- or multiple-dimensional chromatography.
authors.affiliation: Department of Molecular Microbiology and Immunology, USC Norris Cell and Tissue Imaging Core, USC/Norris Comprehensive Cancer Center, Keck School of Medicine at USC, Los Angeles, CA, USA.
authors.forename: Ling-Chi
authors.initials: LC
authors.lastname: Wang
authors.forename: Cindy Yen
authors.initials: CY
authors.lastname: Okitsu
authors.forename: Harold
authors.initials: H
authors.lastname: Kochounian
authors.forename: Anthony
authors.initials: A
authors.lastname: Rodriguez
authors.forename: Chih-Lin
authors.initials: CL
authors.lastname: Hsieh
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Zandi
mesh_terms: D000818:Animals; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D044367:Phase Transition; D040901:Proteomics / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
chemical_list: D012822:Silicon Dioxide