title: TLC analysis of intermediates arising during the preparation of oxime HI-6 dimethanesulfonate.
abstract: In this study, several thin-layer chromatography (TLC) methods are described for the identification of quaternary and non-quaternary compounds (parent compounds, intermediates, by-products, and products) arisen within the synthesis of the acetylcholinesterase reactivator HI-6, at present the most promising antidote in the case of nerve agent poisonings. Using the TLC technique, particular E and Z isomers of this compound on the oxime group are separated. These TLC methods could be of high interest as quick purity control for those who are interested in development of new acetylcholinesterase reactivators and the synthesis of HI-6 in laboratories or in large-scale production.
authors.affiliation: Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Jun
authors.forename: Petr
authors.initials: P
authors.lastname: Stodulka
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
authors.forename: Vit
authors.initials: V
authors.lastname: Koleckar
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Dolezal
authors.forename: Peter
authors.initials: P
authors.lastname: Simon
authors.forename: Miroslav
authors.initials: M
authors.lastname: Veverka
mesh_terms: D002801:Cholinesterase Reactivators / Q000138:chemical synthesis / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D008698:Mesylates / Q000302:isolation & purification*; D010091:Oximes / Q000302:isolation & purification*; D011726:Pyridinium Compounds / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D002801:Cholinesterase Reactivators; D008698:Mesylates; D010091:Oximes; D011726:Pyridinium Compounds; C022870:asoxime chloride