[Detection of autoantibodies in the sera of nasopharyngeal carcinoma patients].
OBJECTIVE
To investigate whether there are autoantibodies to nasopharyngeal carcinoma (NPC) in the sera of patients and to find new NPC biomarkers.


METHODS
Cell plate of Epstein Barr virus negative NPC cell line CNE1 was prepared, and difference between 32 NPC patient sera and 54 normal sera was analyzed by ELISA. We extracted the total protein of CNE1, and analyzed whether there were specific proteins to react with NPC sera by Western blot.


RESULTS
The average absorbance values of serum antibody in NPC patients (0.904+/-0.032) were significantly higher than those of normal serum antibody absorbance values (0.736+/-0.028) (P< 0.01). The analysis with Western blot showed there were positive bands,and some of these were unanimous bands, but the intensity increased, and some of these were new bands compared with the normal sera. These positive bands may be NPC tumor-associated antigens or NPC tumor-specific antigens.


CONCLUSIONS
Autoantibodies that react with NPC exist in the sera of NPC patients, but they do not react with Epstein-Barr virus. It provides the basis to seek the tumor biomarkers in NPC sera.
Tong
Yong-qing
YQ
Cancer Research Institute, Central South University, Changsha 410078, China.
Huang
Jian
J
Zhou
Guo-hua
GH
Li
Yue-hui
YH
Hu
Jin-yue
JY
Li
Guan-cheng
GC
D000951:Antigens, Neoplasm / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000276:immunology*
D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor