title: Free radical activity and lipid soluble anti-oxidant vitamin status in patients with long-term ileal pouch-anal anastomosis.
abstract: OBJECTIVE
  Ileal pouch-anal anastomosis (IPAA) is the operation of choice for patients with ulcerative colitis. Free radical activity and the status of lipid soluble antioxidant vitamins have not been previously assessed in patients with IPAA. The aim of the present study was to measure the plasma concentrations of lipophyllic antioxidants and free radical activity in IPAA patients and compare them with normal subjects.
  
  
  METHODS
  Forty-eight IPAA patients and 50 healthy controls were studied. A dietary assessment of vitamin E (alpha-tocopherol) and carotene was undertaken and plasma antioxidant status was assessed. Plasma malondialdehyde (MDA) was measured to assess the extent of free radical damage. In IPAA patients, association between the degree of inflammation in the pouch mucosa and the plasma concentration of lipophyllic antioxidants and extent of free radical activity was investigated.
  
  
  RESULTS
  The dietary intake of carotene was similar in both groups. Intake of vitamin E was significantly lower in patients than controls (P = 0.01). In the IPAA group plasma concentrations of alpha-carotene, beta-carotene and lycopene were significantly lower (P < 0.001) and alpha-tocopherol:cholesterol ratio significantly higher (P < 0.001). Free radical damage was significantly greater in patients than controls (P < 0.01). There were no significant correlations between the degree of inflammation in the pouch and plasma concentrations of MDA, carotenoids, alpha-tocopherol:cholesterol ratio or intake of vitamins.
  
  
  CONCLUSIONS
  Compared with normal subjects, patients with IPAA have significantly lower plasma concentrations of lipophyllic antioxidants alpha-carotene, beta-carotene and lycopene and higher free radical activity suggesting increased oxidative stress. These differences do not appear to be related to diet and do not correlate with histological severity of pouch inflammation.
authors.affiliation: Department of Coloproctology, Lister Department of Surgery, Glasgow Royal Infirmary.
authors.forename: M S
authors.initials: MS
authors.lastname: El Muhtaseb
authors.forename: D
authors.initials: D
authors.lastname: Talwar
authors.forename: A
authors.initials: A
authors.lastname: Duncan
authors.forename: D
authors.initials: D
authors.lastname: St J O'reilly
authors.forename: R F
authors.initials: RF
authors.lastname: McKee
authors.forename: J H
authors.initials: JH
authors.lastname: Anderson
authors.forename: A
authors.initials: A
authors.lastname: Foulis
authors.forename: I G
authors.initials: IG
authors.lastname: Finlay
mesh_terms: D000328:Adult; D000368:Aged; D000714:Anastomosis, Surgical; D002338:Carotenoids / Q000097:blood*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000601:surgery; D039021:Colonic Pouches / Q000009:adverse effects* / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007249:Inflammation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D014810:Vitamin E / Q000097:blood*; D055815:Young Adult
chemical_list: D014810:Vitamin E; D002338:Carotenoids; D008315:Malondialdehyde