Preparation and cytocompatibility of PLGA scaffolds with controllable fiber morphology and diameter using electrospinning method.
Poly(lactide-co-glycolide) (PLGA) scaffolds prepared by the electrospinning technology were paid more attention in tissue engineering, but detailed investigation on the influence of process parameters on scaffold morphology and fiber diameters as well as distribution remains to be conducted. In the current study, PLGA concentration, electric field strength, and feeding rate were varied to prepare different PLGA scaffolds. It was shown that with the varying of the above parameters, the electrospun porous PLGA scaffolds exhibited fibrous morphology either with/out beads or bead-fiber string. The diameter of nanofibers increased with the PLGA concentration and feeding rate, whereas the electric field strength exerted only minor effects on the average diameter of the nanofibers. The cytocompatibility of the scaffold prepared from optimizing fabrication parameters was evaluated using human fibroblasts. Good cell attachment, fast proliferation rate, characteristic fibroblast morphology with homogeneous distribution was observed on the scaffold. As a whole, this study demonstrated that the fiber morphology and diameter of the electrospun PLGA scaffolds could be tailored by controlling fabrication parameters. And nanofibrous PLGA could act as a biologically preferred scaffold for fibroblasts growth with maintenance of characteristic morphology.
Zhao
Li
L
Shanghai Tissue Engineering Research and Development Center, Shanghai 200235, China. jasmine_zhao@hotmail.com
He
Chenguang
C
Gao
Yongjuan
Y
Cen
Lian
L
Cui
Lei
L
Cao
Yilin
Y
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000627:therapeutic use; D049329:Nanostructures / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000627:therapeutic use; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid