Lupane triterpenes with a carbonyl group at C-20 induce cancer cell apoptosis.
We evaluated the effects of various lupane triterpenes on B16 2F2 mouse melanoma cell differentiation and proliferation. All of the compounds tested (numbered 1-6) induced melanogenesis of B16 2F2 cells, a marker of melanoma cell differentiation. Compounds 4-6, which have a carbonyl group at C-20, markedly inhibited the growth of B16 2F2 cells by the induction of apoptosis. Cytotoxic profiles of these lupane triterpenes against human cancer cells demonstrated that compounds 4-6 showed inhibitory effects on the proliferations of leukemia and lung cancer cells, to a greater extent than other cancer and normal fibroblast cells. These results suggest that the carbonyl group at C-20 of lupane triterpenes played important roles in their apoptosis-inducing activity against cancer cells.
Hata
Keishi
K
Institute for Food & Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, 4-26 Sanuki, Araya-machi, Akita, 010-1623, Japan. hata@arif.pref.akita.jp
Ogawa
Shoujiro
S
Makino
Mitsuko
M
Mukaiyama
Toshiyuki
T
Hori
Kazuyuki
K
Iida
Takashi
T
Fujimoto
Yasuo
Y
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D010936:Plant Extracts / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
D008543:Melanins; D010936:Plant Extracts; D014315:Triterpenes