Carbonyl cyanide m-chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H(+)-ATPase in the yeast Saccharomyces cerevisiae.
The plasma membrane H(+)-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H(+)-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase C-dependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H(+)-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase.
Pereira
Michele B P
MB
Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, Ouro Preto, MG - Brazil.
Tisi
Renata
R
Fietto
Luciano G
LG
Cardoso
Anamaria S
AS
França
Mônica M
MM
Carvalho
Fernanda M
FM
Trópia
Maria José M
MJ
Martegani
Enzo
E
Castro
Ieso M
IM
Brandão
Rogelio L
RL
D002118:Calcium / Q000032:analysis; D020013:Calcium Signaling / Q000187:drug effects*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology*; D003600:Cytosol / Q000737:chemistry; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
D044385:GTP-Binding Protein alpha Subunits; D007476:Ionophores; D029701:Saccharomyces cerevisiae Proteins; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D011493:Protein Kinase C; D010738:Type C Phospholipases; D006180:Proton-Translocating ATPases; C083907:Gpa2 protein, S cerevisiae; D002118:Calcium