Novel halophilic aerobic anoxygenic phototrophs from a Canadian hypersaline spring system.
The first enumeration of cultivable obligately aerobic phototrophic bacteria from a terrestrial saline spring was accomplished in the East German Creek system (salinity approximately 6%), near Lake Winnipegosis, Manitoba, Canada. Occurring at densities up to 3.3x10(7) CFU/ml of sample, aerobic phototrophs comprised 15-36% of the total cultivable bacterial population in the diatom- and chlorophyte-dominated aerobic microbial mats. Many of the representative strains isolated for phenotypic characterization and phylogenetic analysis possessed <96% 16S rDNA sequence overlap with published species, including an obligately aerobic phototrophic gammaproteobacterium displaying only 92.9% 16S rDNA sequence similarity to Congregibacter litoralis. The springs yielded the most highly halotolerant aerobic anoxygenic phototroph yet recorded, strain EG11, which grew with 26% NaCl.
Csotonyi
Julius T
JT
Department of Microbiology, University of Manitoba, 418 Buller Building, R3T 2N2, Winnipeg, MB, Canada.
Swiderski
Jolantha
J
Stackebrandt
Erko
E
Yurkov
Vladimir V
VV
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000737:chemistry; D002170:Canada; D002244:Carbon / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017377:Diatoms; D005843:Geography; D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D010802:Phylogeny; D012492:Salts / Q000737:chemistry / Q000494:pharmacology*; D013234:Stem Cells; D013696:Temperature; D014867:Water / Q000737:chemistry
D001429:Bacteriochlorophylls; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012492:Salts; D014867:Water; D002244:Carbon