title: PCB 118 and Aryl Hydrocarbon Receptor Immunoassays for Screening Dioxins in Retail Fish.
abstract: The efficacy of a combination of two enzyme-linked immunosorbent assay (ELISA) kits was examined for screening the toxic equivalent (TEQ) concentrations of dioxins in retail fish. The coplanar PCB-EIA system, which is a competitive immunoassay specific for polychlorinated biphenyl (PCB) 118, was tested as a screening method for mono- ortho PCBs. The Ah immunoassay (Ah-I), which is an ELISA-based aryl hydrocarbon receptor binding assay, was analyzed for its screening ability for non- ortho PCBs, polychlorinated dibenzo- p-dioxins (PCDDs), and dibenzofurans (PCDFs). Dilution and recovery tests using purified fish extracts revealed no major interference of the matrix in the PCB-EIA and suggested that the matrix effect was minimized in the Ah-I. Finally, the results for the fish samples ( n = 20) showed a strong correlation between this method and high-resolution gas chromatography coupled to high-resolution mass spectrometry for the determination of the TEQ concentrations of mono- ortho PCBs ( r = 0.99) and non- ortho PCBs and PCDD/Fs ( r = 0.97). These data indicate that our method is suitable for screening retail fish to determine the TEQ concentrations of dioxins.
authors.affiliation: National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan. tutumi@nihs.go.jp
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Tsutsumi
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Amakura
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ashieda
authors.forename: Akira
authors.initials: A
authors.lastname: Okuyama
authors.forename: Youhei
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Kazuto
authors.initials: K
authors.lastname: Sakata
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Kumiko
authors.initials: K
authors.lastname: Sasaki
authors.forename: Tamio
authors.initials: T
authors.lastname: Maitani
mesh_terms: D000818:Animals; D004147:Dioxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005399:Fishes; D008460:Meat / Q000032:analysis*; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D004147:Dioxins; D018336:Receptors, Aryl Hydrocarbon; C070055:2,3',4,4',5-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls