title: Is delta-aminolevulinic acid dehydratase activity in bivalves from south-west Iberian Peninsula a good biomarker of lead exposure?
abstract: The coast of Huelva is considered as a heavily contaminated area where the Tinto and Odiel rivers discharge after running through a metalliferous mining area in the Iberian Pyrite Belt and end in common estuary called Ría of Huelva. Lead is a highly toxic and widely distributed element in the aquatic environment; therefore there is a great interest in assessing the impact of this contaminant on aquatic organisms. To study the bioavailability and sources of lead, the bivalve species Chamelea gallina was collected from six sampling sites along the coast of Huelva. Besides the effect of this metal on the enzyme activity, delta-aminolevulinic acid dehydratase (ALA-D) was determined in the whole tissue. Results show the inverse relationship between the lead concentration and the activity of ALA-D measured in whole tissues (r2=0.7). Individuals collected from the Ría of Huelva estuary had the highest levels of total lead concentrations and the lowest activity of ALA-D. Lead isotope analyses demonstrated the different sources of lead contamination and also confirmed the influence of Ría of Huelva on the metal input to the marine environment.
authors.affiliation: Instituto de Ciencias Marinas de Andalucía, CSIC Polígono Río San Pedro s/n, Puerto Real Cádiz, Spain. judit.kalman@vcd.es
authors.forename: Judit
authors.initials: J
authors.lastname: Kalman
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Riba
authors.forename: Julián
authors.initials: J
authors.lastname: Blasco
authors.forename: T Angel
authors.initials: TA
authors.lastname: DelValls
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049872:Bivalvia / Q000201:enzymology*; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D007854:Lead / Q000008:administration & dosage* / Q000032:analysis / Q000494:pharmacology*; D009792:Oceans and Seas; D000623:Porphobilinogen Synthase / Q000378:metabolism*; D013030:Spain
chemical_list: D015415:Biomarkers; D007854:Lead; D000623:Porphobilinogen Synthase