title: Crystallization and preliminary X-ray diffraction analysis of the glucuronoyl esterase catalytic domain from Hypocrea jecorina.
abstract: The catalytic domain of the glucuronoyl esterase from Hypocrea jecorina (anamorph Trichoderma reesei) was overexpresssed, purified and crystallized by the sitting-drop vapor-diffusion method using 1.4 M sodium/potassium phosphate pH 6.9. The crystals belonged to space group P2(1)2(1)2(1) and X-ray diffraction data were collected to 1.9 A resolution. This is the first enzyme with glucoronoyl esterase activity to be crystallized; its structure will be valuable in lignocellulose-degradation research.
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Wood
authors.forename: X-L
authors.initials: XL
authors.lastname: Li
authors.forename: M A
authors.initials: MA
authors.lastname: Cotta
authors.forename: P
authors.initials: P
authors.lastname: Biely
authors.forename: N E C
authors.initials: NE
authors.lastname: Duke
authors.forename: M
authors.initials: M
authors.lastname: Schiffer
authors.forename: P R
authors.initials: PR
authors.lastname: Pokkuluri
mesh_terms: D020134:Catalytic Domain / Q000235:genetics; D003460:Crystallization; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D005965:Glucuronates / Q000737:chemistry* / Q000378:metabolism; D035901:Hypocrea / Q000201:enzymology*; D011994:Recombinant Proteins / Q000737:chemistry; D013379:Substrate Specificity; D014242:Trichoderma / Q000201:enzymology; D014961:X-Ray Diffraction
chemical_list: D005656:Fungal Proteins; D005965:Glucuronates; D011994:Recombinant Proteins; D004950:Esterases