Induction and reversibility of insulin resistance in rats exposed to exogenous D-fructose.
Long-term exposure of normal rats to a fructose-enriched diet or drinking water is currently used as an animal model for experimental insulin resistance. The present study deals with a comparison between rats given access to either a fructose-enriched diet or fructose-enriched drinking water. In both situations, a decrease in food intake and body weight gain, and the induction of insulin resistance with intolerance to D-glucose despite increased secretory response to the aldohexose of insulin-producing cells were documented. Moreover, the rats exposed to exogenous D-fructose displayed a lesser sensitivity to overnight fasting than control animals, in terms of the alteration of glucose homeostasis and reduction of the ratio between plasma insulin and D-glucose concentration. It is also shown that the fructose-induced insulin resistance, as assessed in a hyperinsulinemic-euglycemic clamp, represents a phenomenon reversed within 15-30 days after removal of the keto-hexose from the drinking water.
Cancelas
J
J
Department of Metabolism, Nutrition and Hormones, Fundacíón Jiménez Díaz, Madrid, Spain.
Prieto
P G
PG
García-Arévalo
M
M
Sancho
V
V
Villanueva-Peñacarrillo
M L
ML
Malaisse
W J
WJ
Valverde
I
I
D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004032:Diet / Q000009:adverse effects; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects / Q000502:physiology; D005632:Fructose / Q000009:adverse effects* / Q000494:pharmacology; D005951:Glucose Tolerance Test / Q000379:methods; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance / Q000502:physiology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013217:Starvation / Q000378:metabolism; D013997:Time Factors; D015430:Weight Gain / Q000187:drug effects
D001786:Blood Glucose; D007328:Insulin; D005632:Fructose