title: Effects of physical and chemical treatments upon biophysical properties and micro-relief of human skin.
abstract: The aim of the present study was to determine the attendant effects of physical (tape-stripping) and chemical (three commercial hydrating formulations) treatments upon biophysical and micro-relief properties of human skin. In the first set of experiment, the effects of tape-stripping onto human stratum corneum (SC) biophysical and micro-relief properties were assessed in nine volunteers. Transepidermal water loss (TEWL), skin hydration and micro-relief parameters (including total length of the lines in mm per mm(2); total surface in %; roughness of the skin measured in gray level (Ra); maximum profile valley (Rv) depth; maximum profile peak height (Rp); maximum height (Rt), peak density (Pc) and coefficient of anisotropy) were determined by using SkinEvidence Pro after subsequent tape-stripping of SC. The relevance of roughness determination as gray level by SkinEvidence Pro was confirmed by using surface roughness standards. In the second set of experiment, the effectiveness of three commercial hydrating formulations onto human SC biophysical parameters and micro-relief properties were assessed in six volunteers. TEWL, hydration and micro-relief parameters were assessed onto pre-treated acetone skin and then treated by three commercial hydrating formulations after 2, 4 and 6 h skin exposure. A linear relation between hydration and cutaneous parameters (total length of the lines, Ra and Rp) as function of SC removed was shown. Skin barrier properties evaluated by TEWL measurements, were not modified by topical formulations. However, skin treated by topical formulations showed slightly higher hydration than the one determined in control group, while micro-relief parameters were not modified. In this study was showed that biophysical and micro-relief parameters were closely related in tape-stripping experiment. Efficiency of topical formulations was suggested upon skin hydration but not onto skin micro-relief and barrier function recovering. From both experiments, it appears that different mechanisms relating to skin hydration and potential modification of cutaneous micro-relief were suggested.
authors.affiliation: Laboratoire de Recherche et Développement de Pharmacie Galénique Industrielle, EA 4169 Fonctions normales et pathologiques de la barrière cutanée, Faculté de Pharmacie, Université Claude Bernard Lyon 1, Lyon Cedex 08, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Atrux-Tallau
authors.forename: Ngoc T T
authors.initials: NT
authors.lastname: Huynh
authors.forename: Laurie
authors.initials: L
authors.lastname: Gardette
authors.forename: Cyril
authors.initials: C
authors.lastname: Pailler-Mattéi
authors.forename: Hassan
authors.initials: H
authors.lastname: Zahouani
authors.forename: Eric
authors.initials: E
authors.lastname: Viviant
authors.forename: Henri
authors.initials: H
authors.lastname: Hirsch
authors.forename: Haftek
authors.initials: H
authors.lastname: Marek
authors.forename: Françoise
authors.initials: F
authors.lastname: Falson
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pirot
mesh_terms: D000287:Administration, Topical; D000328:Adult; D003879:Dermatologic Agents / Q000008:administration & dosage*; D046169:Dermoscopy; D004655:Emulsions; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D020127:Recovery of Function / Q000187:drug effects; D012867:Skin / Q000033:anatomy & histology / Q000737:chemistry* / Q000187:drug effects*; D013499:Surface Properties; D053832:Surgical Tape / Q000706:statistics & numerical data* / Q000639:trends; D014870:Water Loss, Insensible / Q000187:drug effects*; D014906:Wetting Agents / Q000008:administration & dosage*
chemical_list: D003879:Dermatologic Agents; D004655:Emulsions; D014906:Wetting Agents