title: Isolation and characterization of new cyclohexylacetic acid-degrading bacteria.
abstract: Six cyclohexylacetic acid-degrading strains were isolated from soil samples in Japan and identified as members of the genera Cupriavidus (strain KUA-1), Rhodococcus, and Dietzia by 16S rRNA gene sequence analysis. For the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. A selected strain, KUA-1, which is the first reported Gram-negative organism capable of growth on cyclohexylacetic acid, was identified as a Cupriavidus metallidurans, based on morphologic and physiologic characteristics and its 16S rRNA gene sequence. Metabolite analysis by HPLC-MS indicated that 1-cyclohexenylacetic acid is an intermediate of cyclohexaneacetic acid metabolism in strain KUA-1.
authors.affiliation: Department of Life Science and Biotechnology, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Iwaki
authors.forename: Emiko
authors.initials: E
authors.lastname: Nakai
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Hasegawa
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000192:Actinomycetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D002851:Chromatography, High Pressure Liquid; D049921:Cupriavidus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003510:Cyclohexanes; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology
chemical_list: D000085:Acetates; D003510:Cyclohexanes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C018883:cyclohexaneacetic acid