title: Protective effects of phenolic fraction of blue honeysuckle fruits against UVA-induced damage to human keratinocytes.
abstract: In this study, the UVA (photo)protective activity of the phenolic fraction of L. caerulea fruits (PFLC) was assessed in human keratinocytes HaCaT. The keratinocytes were pre- or post-treated with PFLC (1-250 mg/l) and exposed to UVA irradiation (10-30 J/cm(2)). The results showed that both pre- and post-treatment with PFLC significantly suppressed UVA-induced ROS production, which was also revealed as a decrease in intracellular lipid peroxidation and elevation of reduced glutathione. Protection was concentration-dependent with a maximum at 50 mg/l. These results suggest that PFLC attenuates UVA-induced oxidative stress by reduction of ROS generation and ROS-mediated damage. For this reason, PFLC has potentially skin-protective functions against the deleterious effects of sunlight.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Czech Republic. alf.svoboda@seznam.cz
authors.forename: Alena
authors.initials: A
authors.lastname: Svobodová
authors.forename: Jana
authors.initials: J
authors.lastname: Rambousková
authors.forename: Daniela
authors.initials: D
authors.lastname: Walterová
authors.forename: Jitka
authors.initials: J
authors.lastname: Vostálová
mesh_terms: D002461:Cell Line, Transformed; D016166:Free Radical Scavengers / Q000378:metabolism; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D031239:Lonicera; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D019800:Phenol / Q000737:chemistry; D008517:Phytotherapy; D010936:Plant Extracts / Q000138:chemical synthesis / Q000494:pharmacology*; D011832:Radiation Injuries / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000528:radiation effects; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D019800:Phenol; D005978:Glutathione