[Study on genetic polymorphisms of P-selectins in Chinese Han of Chengdu and Thai populations].
OBJECTIVE
To study the gene polymorphisms of position --2123 C/G,--1969 G/A,--1817 T/C in promoter region and of Thr715Pro in exon thirteenth of P-selectin in the Chinese Han of Chengdu and Thai populations, and simultaneously to compare distributions of genotype and allelic frequencies of P-selectins among different races. Methods The genotypes and allele frequencies of the P-selectin base --2123 C/G,--1969 G/A,-1817 T/C and amino acid Thr715Pro were detected by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) to 120 healthy Chinese Han of Chengdu and 110 Thai population.


RESULTS
There were no significant differences in the genotype and allele distribution of--2123 C/G,--1969 G/A,--1817 T/C polymorphisms for the P-selectin gene between Chinese Han of Chengdu and Thai populations (P > 0.05), in which compared with England and American, the distribution of P-selectin genotype and allele had significantly differences among ethnics (P < 0.001). No polymorphism of Thr715Pro was found in this study. Conclusion In Chinese Han of Chengdu and Thai populations the polymorphisms exist at base position--2123 C/G,--1969 G/A and --1817 T/C in promoter region of P-selectin. There are no significant differences in the genotype and allele distribution of the P-selectin gene polymorphisms between Chinese Han of Chengdu and Thai populations, but significantly different distribution of P-selectin gene polymorphisms occur among ethnics.
Wei
Ye-sheng
YS
Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Gao
Lin-bo
LB
Zhu
Yin-hua
YH
Tai
Shu-hong
SH
Yang
Zhi-hui
ZH
Liang
Wei-bo
WB
Lu
Mei-li
ML
Zhang
Lin
L
D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D019007:P-Selectin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013785:Thailand / Q000208:ethnology
D019007:P-Selectin