title: Analysis of appositional bone formation using a novel rat experimental model.
abstract: OBJECTIVE
  To analyze the process of appositional bone formation using our original rat experimental model.
  
  
  METHODS
  Rats were anesthetized and a ring made of polytetrafluorethylene was placed on the parietal bone surface in the surgical procedure. The time course of appositional bone formation was analyzed with histomorphometry and in situ hybridization for type I collagen and bone sialoprotein.
  
  
  RESULTS
  The rat experimental model allowed new bone to be formed on the pre-existing bone surface and persist for 12 weeks. We demonstrated that bone is apposed actively for the first 4 weeks and less actively thereafter.
  
  
  CONCLUSIONS
  The experimental model may contribute to biological analysis for appositional bone formation expected to occur in clinical procedures such as alveolar bone augmentation and sinus lifting.
authors.affiliation: Divisions of Oral Surgery, and Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Nakajima
authors.forename: S
authors.initials: S
authors.lastname: Kamakura
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: O
authors.initials: O
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Echigo
authors.forename: Y
authors.initials: Y
authors.lastname: Sasano
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000502:physiology; D001861:Bone Regeneration / Q000502:physiology*; D024042:Collagen Type I / Q000096:biosynthesis; D017403:In Situ Hybridization; D058574:Integrin-Binding Sialoprotein; D008297:Male; D008567:Membranes, Artificial; D023421:Models, Animal; D010006:Osteoblasts / Q000378:metabolism; D010294:Parietal Bone / Q000601:surgery; D010521:Periosteum / Q000502:physiology; D011138:Polytetrafluoroethylene; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000096:biosynthesis
chemical_list: D024042:Collagen Type I; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D008567:Membranes, Artificial; D012795:Sialoglycoproteins; D011138:Polytetrafluoroethylene