title: Analysis of a rapid, simple, and inexpensive technique used to obtain platelet-rich plasma for use in clinical practice.
abstract: The use of platelet-rich plasma (PRP) has become more generally accepted, and implant dentists are using PRP more frequently to promote the healing of oral surgical and/or periodontal wounds. Critical elements of PRP are thought to be growth factors contained within the concentrated platelets. These growth factors are known to promote soft-tissue healing, angiogenesis and osteogenesis. We present a rapid, simple, and inexpensive methodology for preparing PRP using the Cliniseal centrifuge method. This study demonstrates that platelets are concentrated approximately 6-fold without altering platelet morphology. Further we demonstrate that key growth factors, platelet-derived growth factor BB (PDGF-BB), transforming growth factor B (TGF-B1), vasculature endothelial growth factor (VEGF), and epidermal growth factor (EGF) are present in comparable or higher concentrations than those reported with the use of other techniques. Prolonged bench set time (>3 hours) after centrifugation resulted in decreased concentration of TGF-B1 but not decreased concentration of PDGF-BB, VEGF, or EGF. This study confirms the molecular aspects of PRP obtained using this inexpensive and efficient methodology.
authors.affiliation: Duquesne University, Pittsburgh, Pa., USA. james.rutkowski@verizon.net
authors.forename: James L
authors.initials: JL
authors.lastname: Rutkowski
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Thomas
authors.forename: C Larry
authors.initials: CL
authors.lastname: Bering
authors.forename: Julie L
authors.initials: JL
authors.lastname: Speicher
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Radio
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Smith
authors.forename: David A
authors.initials: DA
authors.lastname: Johnson
mesh_terms: D001792:Blood Platelets; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002498:Centrifugation / Q000379:methods; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D053657:Platelet-Rich Plasma
chemical_list: D006133:Growth Substances