The receptor kinase CORYNE of Arabidopsis transmits the stem cell-limiting signal CLAVATA3 independently of CLAVATA1.
Stem cells in shoot and floral meristems of Arabidopsis thaliana secrete the signaling peptide CLAVATA3 (CLV3) that restricts stem cell proliferation and promotes differentiation. The CLV3 signaling pathway is proposed to comprise the receptor kinase CLV1 and the receptor-like protein CLV2. We show here that the novel receptor kinase CORYNE (CRN) and CLV2 act together, and in parallel with CLV1, to perceive the CLV3 signal. Mutations in CRN cause stem cell proliferation, similar to clv1, clv2, and clv3 mutants. CRN has additional functions during plant development, including floral organ development, that are shared with CLV2. The CRN protein lacks a distinct extracellular domain, and we propose that CRN and CLV2 interact via their transmembrane domains to establish a functional receptor.
Müller
Ralf
R
Institut für Genetik, Heinrich-Heine University, D-40225 Düsseldorf, Germany.
Bleckmann
Andrea
A
Simon
Rüdiger
R
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D017931:DNA Primers; D017403:In Situ Hybridization; D018519:Meristem / Q000254:growth & development; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified; D017346:Protein Serine-Threonine Kinases; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism*
C118167:AT2G27250 protein, Arabidopsis; D029681:Arabidopsis Proteins; D017931:DNA Primers; D020794:Receptor Protein-Tyrosine Kinases; C118166:CLV1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases