title: Identification of 3,4-didehydrorhodopin as major carotenoid in Rhodopseudomonas species.
abstract: Recently we isolated the purple photosynthetic bacterium, Rhodopseudomonas sp. Rits, which was phylogenetically related to Rhodopseudomonas (Rps.) palustris. In this study, the light-dependent and time-dependent changes in the carotenoid composition were investigated by HPLC analysis of extracts from the cultures. All seven carotenoids in the biosynthetic pathway from lycopene to spirilloxanthin were detected. Especially, 3,4-didehydrorhodopin, having twelve conjugated double bonds as well as one terminal hydroxy group, was isolated in a remarkably large amount and fully characterized for the first time. The biosynthetic intermediate was commonly found in the Rps. palustris strains (CGA009, Morita and NBRC100419).
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Mizoguchi
authors.forename: Megumi
authors.initials: M
authors.lastname: Isaji
authors.forename: Jiro
authors.initials: J
authors.lastname: Harada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tamiaki
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D050296:Microbial Viability; D015394:Molecular Structure; D012241:Rhodopseudomonas / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
chemical_list: C529420:3,4-didehydrorhodopin; D002338:Carotenoids