Absence of 2'-deoxyguanosine-carbon 8-bound ochratoxin A adduct in rat kidney DNA monitored by isotope dilution LC-MS/MS.
The contribution of DNA adduct formation in the carcinogenic action of the mycotoxin ochratoxin A (OTA) has been subject to much debate. Recently, a carbon-bonded ochratoxin A-2'-deoxyguanosine adduct (dGuoOTA) formed by photochemical reaction in vitro has been shown by 32P-postlabeling/TLC to comigrate with a spot detected in DNA isolated from rat and pig kidney following exposure to OTA. Considering the large body of evidence arguing against covalent DNA binding of OTA and the poor resolution and specificity of postlabeling analysis, we developed a stable isotope dilution LC-MS/MS method to analyze dGuoOTA in kidney DNA isolated from rats treated with OTA. dGuoOTA and nitrogen-15-labeled dGuoOTA (15N(5)-dGuoOTA) were prepared by photoirradiation of OTA in the presence of dGuo or nitrogen-15-labeled dGuo. Conditions for DNA hydrolysis were optimized using a synthetic oligonucleotide containing dGuoOTA to ensure complete release of dGuoOTA. The LOD of the method (S/N > 3) was 10 fmol dGuoOTA on-column. However, dGuoOTA was not detected in DNA samples isolated from male F344 rats treated with OTA for up to 90 days at doses known to cause renal tumor formation. Detection limits, calculated for each individual sample based on the absolute LOD and the amount of DNA injected, were as low as 3.5 dGuoOTA/10(9) nucleotides. These data are consistent with previous results showing lack of DNA adduct formation by OTA and demonstrate that dGuoOTA is not formed in biologically relevant amounts under physiological conditions in vivo.
Delatour
Thierry
T
Institut für Toxikologie, Universität Würzburg, Würzburg, Germany. thierry.delatour@rdls.nestle.com
Mally
Angela
A
Richoz
Janique
J
Ozden
Sibel
S
Dekant
Wolfgang
W
Ihmels
Heiko
H
Otto
Daniela
D
Gasparutto
Didier
D
Marin-Kuan
Maricel
M
Schilter
Benoît
B
Cavin
Christophe
C
D000284:Administration, Oral; D000818:Animals; D002853:Chromatography, Liquid; D004247:DNA / Q000187:drug effects* / Q000302:isolation & purification; D003849:Deoxyguanosine / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000502:physiology*; D008297:Male; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D009183:Mycotoxins / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D009711:Nucleotides / Q000737:chemistry; D009793:Ochratoxins / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D009183:Mycotoxins; D009711:Nucleotides; D009793:Ochratoxins; C025589:ochratoxin A; D004247:DNA; D003849:Deoxyguanosine