Sodium arsenite reduces severity of dextran sulfate sodium-induced ulcerative colitis in rats.
The histopathological features and the associated clinical findings of ulcerative colitis (UC) are due to persistent inflammatory response in the colon mucosa. Interventions that suppress this response benefit UC patients. We tested whether sodium arsenite (SA) benefits rats with dextran sulfate sodium (DSS)-colitis. The DSS-colitis was induced by 5% DSS in drinking water. SA (10 mg/kg; intraperitoneally) was given 8 h before DSS treatment and then every 48 h for 3 cycles of 7, 14 or 21 d. At the end of each cycle rats were sacrificed and colon sections processed for histological examination. DSS induced diarrhea, loose stools, hemoccult positive stools, gross bleeding, loss of body weight, loss of epithelium, crypt damage, depletion of goblet cells and infiltration of inflammatory cells. The severity of these changes increased in the order of Cycles 1, 2 and 3. Treatment of rats with SA significantly reduced this severity and improved the weight gain.
Malago
Joshua J
JJ
Department of Pathology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, PO Box 3203, Morogoro, Tanzania. malagojj@yahoo.com
Nondoli
Hortensia
H
D000818:Animals; D018053:Arsenites / Q000494:pharmacology*; D001835:Body Weight; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D003093:Colitis, Ulcerative / Q000139:chemically induced*; D003106:Colon / Q000473:pathology; D016264:Dextran Sulfate / Q000494:pharmacology*; D004848:Epithelium / Q000473:pathology; D007249:Inflammation; D008297:Male; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar; D017670:Sodium Compounds / Q000494:pharmacology*; D013997:Time Factors; D016896:Treatment Outcome
D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite; D016264:Dextran Sulfate