Xenopus Paraxial Protocadherin regulates morphogenesis by antagonizing Sprouty.
Xenopus Paraxial Protocadherin (xPAPC) has signaling functions that are essential for convergent extension (CE) movements and tissue separation during gastrulation. PAPC modulates components of the planar cell polarity (PCP) pathway, but it is not clear how PAPC is connected to beta-catenin-independent Wnt-signaling. By yeast two-hybrid screen, we found that the intracellular domain of PAPC interacts with Sprouty (Spry), an inhibitor of CE movements. Upon binding to PAPC, Spry function is inhibited and PCP signaling is enhanced. Our data indicate that PAPC promotes gastrulation movements by sequestration of Spry and reveal a novel mechanism by which protocadherins modulate beta-catenin-independent Wnt-signaling.
Wang
Yingqun
Y
Institute of Human Genetics, University Heidelberg, 69120 Heidelberg, Germany. Yingqun.Wang@med.uni-heidelberg.de
Janicki
Patricia
P
Köster
Isabelle
I
Berger
Corinna D
CD
Wenzl
Christian
C
Grosshans
Jörg
J
Steinbeisser
Herbert
H
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D004330:Drosophila / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007814:Larva / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D000091363:Protocadherins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*; D015003:Yeasts / Q000235:genetics
D048868:Adaptor Proteins, Signal Transducing; D015820:Cadherins; D008565:Membrane Proteins; C117635:Pcdh8 protein, Xenopus; D010750:Phosphoproteins; D000091363:Protocadherins; D011993:Recombinant Fusion Proteins; D029867:Xenopus Proteins; C528766:spry1 protein, Xenopus; D049452:Green Fluorescent Proteins