Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells.
To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.
Takano
Hirotsugu
H
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Nakazawa
Shunsuke
S
Okuno
Yasushi
Y
Shirata
Naritoshi
N
Tsuchiya
Sohken
S
Kainoh
Takayuki
T
Takamatsu
Seigoh
S
Furuta
Kazuyuki
K
Taketomi
Yoshitaka
Y
Naito
Yuko
Y
Takematsu
Hiromu
H
Kozutsumi
Yasunori
Y
Tsujimoto
Gozoh
G
Murakami
Makoto
M
Kudo
Ichiro
I
Ichikawa
Atsushi
A
Nakayama
Kazuhisa
K
Sugimoto
Yukihiko
Y
Tanaka
Satoshi
S
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D006632:Histamine / Q000032:analysis; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D010447:Peptide Hydrolases / Q000378:metabolism; D010537:Peritoneum / Q000166:cytology; D041701:Swiss 3T3 Cells
D006632:Histamine; D010447:Peptide Hydrolases