Incubation type Si-based planar ion channel biosensor.
A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (approximately 7 Mohms) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.
Urisu
Tsuneo
T
Institute for Molecular Science, Myodaiji, Okazaki, 444-8585, Japan. urisu@ims.ac.jp
Asano
Toshifumi
T
Zhang
Zhenlong
Z
Uno
Hidetaka
H
Tero
Ryugo
R
Junkyu
Han
H
Hiroko
Isoda
I
Arima
Yusuke
Y
Iwata
Hiroo
H
Shibasaki
Koji
K
Tominaga
Makoto
M
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002211:Capsaicin / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D007473:Ion Channels / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008024:Ligands; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods; D012825:Silicon / Q000737:chemistry*; D050916:TRPV Cation Channels / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
D007473:Ion Channels; D008024:Ligands; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D002211:Capsaicin; D012825:Silicon