A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system.
Extracellular matrix associated Sparc-like 1 (SC1/SPARCL1) can influence the function of astroglial cells in the developing and mature central nervous system (CNS). To examine SC1's significance in the CNS, we generated a BAC transgenic mouse model in which Sc1 is expressed in radial glia and their astrocyte derivatives using the astroglial-specific Blbp (Brain-lipid binding protein; [Feng et al., (1994) Neuron 12:895-908]) regulatory elements. Characterization of these Blbf-Sc1 transgenic mice show elevated Sc1 transcript and protein in an astroglial selective pattern throughout the CNS. This model provides a novel in vivo system for evaluating the role of SC1 in brain development and function, in general, and for understanding SC1's significance in the fate and function of astroglial cells, in particular.
Weimer
Jill M
JM
UNC Neuroscience Center, The University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA.
Stanco
Amelia
A
Cheng
Jr-Gang
JG
Vargo
Ana C
AC
Voora
Santhi
S
Anton
E S
ES
D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000502:physiology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D023421:Models, Animal
D002135:Calcium-Binding Proteins; D016326:Extracellular Matrix Proteins; C503127:Sparcl1 protein, mouse