Dietary conjugated linoleic acid improves antioxidant capacity in broiler chicks.
1. The influence of dietary conjugated linoleic acid (CLA) on the antioxidant status in the absence or presence of endotoxin exposure was studied with male broiler chicks. 2. In experiment 1, a total of 240 1-d-old broilers were allotted into 4 dietary groups (0, 2.5, 5.0 or 10.0 g pure CLA/kg) to study the influence of CLA on growth performance and antioxidant defence systems. The results showed that growth performance was not altered by 42 d of CLA consumption. Increased total superoxide dismutase (TSOD) activities in liver, serum and muscle were observed in chicks given 10.0 g CLA/kg diet. Dietary CLA at 10.0 g/kg also markedly elevated liver catalase (CAT) activity. Malondialdehyde (MDA), a marker of lipid peroxidation, decreased in liver, serum and muscle in chicks given 5.0 and 10.0 g CLA/kg diet. 3. In experiment 2, a total of 120 1-d-old broilers were fed on a control diet (without CLA) or 10.0 g CLA/kg diet. Half of the birds fed on each diet were injected intraperitoneally with 0.25 mg/kg body weight of Salmonella enteritidis lipopolysaccharide (LPS) at 16, 18 and 20 d of age. Decreased glutathione peroxidase (GSH-Px), TSOD activity and increased ceruloplasmin and MDA concentrations were seen in the challenged chicks. Dietary CLA prevented the loss of body weight gain and feed conversion ratio of chicks followed repeated endotoxin exposure. CLA partially inhibited the increase of serum ceruloplasmin and MDA at 17 and 21 d of age and notably suppressed the decrease of serum TSOD activity at 21 d of age. 4. These results suggested that dietary CLA enhances the activity of antioxidant enzymes including TSOD and CAT. Supplementation of CLA has been shown to ameliorate the antioxidant balance and performance of chicks during oxidative stress.
Zhang
H J
HJ
Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
Tian
Y D
YD
Guo
Y M
YM
Yuan
J M
JM
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000032:analysis*; D002374:Catalase / Q000378:metabolism; D002570:Ceruloplasmin / Q000032:analysis; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D004032:Diet; D005979:Glutathione Peroxidase / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage*; D015227:Lipid Peroxidation; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008099:Liver / Q000201:enzymology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000097:blood; D018482:Muscle, Skeletal / Q000201:enzymology; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood / Q000378:metabolism
D000975:Antioxidants; D044243:Linoleic Acids, Conjugated; D008070:Lipopolysaccharides; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D002570:Ceruloplasmin