title: Study of oxidative stress in type 2 diabetic patients and its relationship with glycated hemoglobin.
abstract: OBJECTIVE
  To evaluate the activity of antioxidant enzymes in diabetic patients and also to determine the correlation between hyperglycemia and lipid peroxidation.
  
  
  METHODS
  Thirty patients with type 2 diabetes and 30 healthy individuals control group participated in this case-control study. The patients were referred to Sina Hospital, Hamadan, Iran from April to June 2006. Glycated hemoglobin HbA1c was measured as a marker of hyperglycemia using the chromatography method Biosystem and malondialdehyde MDA was determined using the colorimetric method. Glutathione peroxidase GPx and superoxide dismutase SOD activity were assessed using the UV-Vis spectrophotometric technique Randox kit.
  
  
  RESULTS
  The mean of HbA1c was higher in diabetic patients compared to the healthy group, and the difference was statistically significant p<0.001. Serum MDA in diabetics was higher compared to those of healthy subjects p<0.001. There were significant differences in activities of SOD and GPx between the 2 studied groups indicating lower activity in diabetic patients p<0.001. There was a significant relationship between MDA and HbA1c in diabetic and healthy subjects.
  
  
  CONCLUSIONS
  The data showed an increase in lipid peroxidation and oxidative stress in diabetes and also indicated a positive correlation between the degree of hyperglycemia and oxidative stress. Evaluation of oxidative status and choosing the appropriate treatment may help to support antioxidant defense in these patients.
authors.affiliation: Department of Biochemistry and Nutrition, Medical School, Hamadan University of Medical Sciences, Hamadan 65178, Iran. mtgoodarzi@yahoo.com
authors.forename: Mohammad T
authors.initials: MT
authors.lastname: Goodarzi
authors.forename: Liela
authors.initials: L
authors.lastname: Varmaziar
authors.forename: Ali A
authors.initials: AA
authors.lastname: Navidi
authors.forename: Kazem
authors.initials: K
authors.lastname: Parivar
mesh_terms: D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D005979:Glutathione Peroxidase; D006442:Glycated Hemoglobin; D008315:Malondialdehyde; D013482:Superoxide Dismutase