title: [Application of flow cytometric immunophenotypic analysis in the diagnosis of malignant lymphoma].
abstract: OBJECTIVE
  To explore the value of flow cytometric immunophenotyping (FCI) in the diagnosis and differentiated diagnosis of lymphoma and explain the immunophenotypic features and differences of malignant lymphoma.
  
  
  METHODS
  Seventy four fresh samples of suspicious lymphoma were collected from Nov. 2004 to Aug. 2006. Each sample was individually evaluated by FCI. The results were analyzed and compared with the histopathological diagnosis.
  
  
  RESULTS
  Among the 74 cases, the FCI data consisted with the final morphological diagnosis in 61 cases (82.4%). For the diagnosis of B and T non-Hodgkin's lymphoma (NHL), thymoma, carcinoma and benign lesions of lymph node, the concordance between FCI data and morphological diagnosis were 93.5%, 100%, 100%, 100% and 81.3%, respectively.
  
  
  CONCLUSIONS
  Multi-parameter FCI analysis can provide important information and help for diagnosis of lymphoma. It is an assistant but necessary approach for the diagnosis and differential diagnosis of lymphoma.
authors.affiliation: Department of Pathology, Cancer Hospital/Institute, CAMS & PUMC, Beijing 100021, China.
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Ren
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xue
authors.forename: Rui
authors.initials: R
authors.lastname: Bi
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Liang
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Lin
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: Lü
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008223:Lymphoma / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged