[Effect of Janus kinase inhibitor AG490 on invasion and metastasis of human breast cancer cells].
OBJECTIVE
To investigate the effect of Janus kinase (JAK) inhibitor AG490 on invasion and metastasis of the human breast cancer cell MDA-MB-231, and to explore the regulating role of JAK-STAT3 signaling pathway when the breast cancer occurs to the invasiveness and metastasis.


METHODS
The human breast cancer cell MDA-MB-231 was used as the research object, and AG490 was as Janus kinase inhibitor. The adhesion of MDA-MB-231 cell attaching to matrigel was measured with MTT assay. The invasion and metastasis potential were evaluated with transwell chamber. The P-STAT3 protein in cell was detected by Western-blot.


RESULTS
Janus kinase inhibitor AG490 could make that the expression of P-STAT3 in human breast cancer cell MDA-MB-231 became weak, and that the abilities of adhesion, invasion and metastasis were also dropping down as compared with the control group (P < 0.01).


CONCLUSIONS
JAK-STAT3 signaling pathway participates in regulating the invasion and metastasis of human breast cancer. Inhibiting the activation of JAK-STAT3 signaling pathway can suppress the invasion and metastasis of human breast cancer.
Chen
Hong-fang
HF
Department of Pathophysiology, Chongqing Medical University, Chongqing 400016, China.
Deng
Hua-yu
HY
Fan
Wei-ke
WK
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D006801:Humans; D053612:Janus Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009362:Neoplasm Metastasis; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D020032:Tyrphostins / Q000494:pharmacology*
D000970:Antineoplastic Agents; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D053612:Janus Kinases