title: Perlecan, the "jack of all trades" proteoglycan of cartilaginous weight-bearing connective tissues.
abstract: Perlecan is a ubiquitous proteoglycan of basement membrane and vascularized tissues but is also present in articular cartilage, meniscus and intervertebral disc, which are devoid of basement membrane and predominantly avascular. It is a prominent pericellular proteoglycan in the transitory matrix of the cartilaginous rudiments that develop into components of diarthrodial joints and the axial skeleton, and it forms intricate perichondrial vessel networks that define the presumptive articulating surfaces of developing joints and line the cartilage canals in cartilaginous rudiments. Such vessels have roles in the nutrition of the expanding cell numbers in the developing joint. Perlecan sequesters a number of growth factors pericellularly (FGFs, PDGF, VEGF and CTGF) and through these promotes cell signalling, cell proliferation and differentiation. Perlecan also interacts with a diverse range of extracellular matrix proteins, stabilising and organising the ECM, and promoting collagen fibrillogenesis. Perlecan is a prominent pericellular component of mesenchymal cells from their earliest developmental stages through to maturation, forming cell-cell and cell-ECM interconnections that are suggestive of a role in mechanosensory processes important to tissue homeostasis.
authors.affiliation: Raymond Purves Research Laboratories, Institute of Bone and Joint Research, Kolling Institute of Medical Research, University of Sydney, Royal North Shore Hospital, St Leonards, NSW, Australia. jmelrose@med.usyd.edu.au
authors.forename: James
authors.initials: J
authors.lastname: Melrose
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Hayes
authors.forename: John M
authors.initials: JM
authors.lastname: Whitelock
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Little
mesh_terms: D000818:Animals; D002356:Cartilage / Q000196:embryology / Q000378:metabolism*; D020219:Chondrogenesis; D003094:Collagen / Q000378:metabolism; D003238:Connective Tissue / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism; D019812:Heparan Sulfate Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D040542:Mechanotransduction, Cellular; D008954:Models, Biological; D010003:Osteoarthritis / Q000378:metabolism; D010012:Osteogenesis; D017434:Protein Structure, Tertiary; D015398:Signal Transduction
chemical_list: D006133:Growth Substances; D019812:Heparan Sulfate Proteoglycans; D008024:Ligands; C071927:perlecan; D003094:Collagen