Kinetin enhances in vitro development of parthenogenetic and nuclear transfer porcine embryos.
Culture conditions affect the development of mammalian embryos in vitro. Kinetin belongs to the family of N(6)-substituted adenine derivates and promotes cell division, synthesis of DNA repair enzymes, superoxide dismutase activity, and ribosomal RNA transcription. We investigated the effects of kinetin on in vitro development of parthenogenetic and nuclear transfer (NT) porcine embryos. These embryos were cultured with or without kinetin in either BSA- or polyvinyl alcohol-containing medium for 7 days. mRNA expression of three developmentally important genes, HSP70, Glut-1, and poly[A] polymerase in NT embryos was analyzed. Regardless of kinetin supplementation, the proportion of blastocysts and blastocyst cells were not significantly different in parthenogenetic embryos. However, kinetin supplementation increased expansion and hatching rates in all groups. In somatic cell NT embryos, kinetin increased the proportion of embryos developed to blastocysts from 7.5% to 15.4% in medium supplemented with PVA. However, gene expression levels of HSP70, poly[A] polymerase and Glut-1 mRNA were not significantly different in NT blastocysts. The present study indicates that kinetin not only improves blastocyst expansion and cell number of parthenogenetic porcine embryos but also enhances NT porcine embryo development in a completely defined culture condition in vitro.
Won
C
C
Dental Research Institute and CLS21, Seoul National University School of Dentistry, Seoul, Republic of Korea.
Park
S K
SK
Cho
S-G
SG
Min
B-M
BM
Roh
S
S
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D046149:Embryo Culture Techniques / Q000379:methods; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D051272:Glucose Transporter Type 1 / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D007701:Kinetin / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D010312:Parthenogenesis; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011062:Polynucleotide Adenylyltransferase / Q000096:biosynthesis; D013552:Swine
D051272:Glucose Transporter Type 1; D018840:HSP70 Heat-Shock Proteins; D010937:Plant Growth Regulators; D011062:Polynucleotide Adenylyltransferase; D007701:Kinetin