Isolation and characterization of a nitrobenzene degrading yeast strain from activated sludge.
Strain Z1 was isolated from nitrobenzene-contaminated sludge. Strain Z1 was able to utilize nitrobenzene as a sole source of carbon, nitrogen, and energy under aerobic condition. Based on the morphology, physiological biochemical characteristics, and 26S rDNA D1/D2 domain sequence, strain Z1 was identified as Rhodotorula mucilaginosa. Strain Z1 mineralized up to 450mg L(-1) nitrobenzene. Kinetics of nitrobenzene degradation was described using the Andrews equation. The kinetic parameters were as follows: q(max)=1.50h(-1), K(s)=31.31mg L(-1), and K(i)=101.34mg L(-1). Strain Z1 had a high-salinity tolerance. It degraded nitrobenzene effectively in 5% NaCl (quality concentration). Even in the presence of aniline or phenol, strain Z1 degraded nitrobenzene efficiently. Strain Z1 therefore could be an excellent candidate for the bio-treatment of nitrobenzene industrial wastewaters.
Chunli
Zheng
Z
School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, Liaoning Province, People's Republic of China. zhengchunli76@163.com
Jiti
Zhou
Z
Jing
Wang
W
Jing
Wang
W
Baocheng
Qu
Q
D000465:Algorithms; D004271:DNA, Fungal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009578:Nitrobenzenes / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D013696:Temperature; D014865:Waste Disposal, Fluid; D015003:Yeasts / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D004271:DNA, Fungal; D009578:Nitrobenzenes; D012722:Sewage; C036077:nitrobenzene