title: [Effect of acupotomy lysis on SP and CCK-8 contents in hypothalamus and spinal cord in rats with transverse process syndrome of the third lumbar vertebra].
abstract: OBJECTIVE
  To observe changes of SP and CCK-8 contents in the hypothalamus and spinal cord after performing acupotomy lysis so as to study its central analgesic effect in rats with transverse-process syndrome of the third lumbar vertebra.
  
  
  METHODS
  Twenty-eight SD rats were randomly divided into normal control group, model group, acupotomy lysis group and electroacupuncture (EA) group, with 7 cases in each one. The model of the third lumbar vertebra transverse process syndrome was established by inserting a piece of gelatin sponge into the deep subfascial part of the 3rd lumbar transverse process. Acupotomy lysis was conducted 1 time/week for 2 weeks. EA (2/100 Hz, 1-2 mA) was applied to the left "Shenshu" (BL 23)-"Yaoyangguan"(GV 3) for 20 min/time, 3 times/week and for 2 weeks. SP and CCK-8 contents in the hypothalamus and spinal cord were detected with enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  In model group, SP and CCK-8 contents in the hypothalamus and spinal cord were increased significantly in comparison with normal control group (P < 0.01). Compared with model group, both SP and CCK-8 contents in hypothalamus and SP content in spinal cord in acupotomy lysis and EA groups were apparently lower (P < 0.01, 0.05). CCK-8 content in spinal cord in cupotomy lysis group was lower markedly than that of model group (P < 0.01), while no significant difference was found between model group and EA group in CCK-8 level in spinal cord (P > 0.05).
  
  
  CONCLUSIONS
  Acupotomy lysis can downregulate SP and CCK-8 contents in hypothalamus and spinal cord, which may contribute to its effect in relieving pain from transverse process syndrome of the third lumbar vertebra.
authors.affiliation: College of Acu-moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Guo
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Li
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Sun
authors.forename: Nai-gang
authors.initials: NG
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Shi-zhe
authors.initials: SZ
authors.lastname: Gu
authors.forename: Hui-fang
authors.initials: HF
authors.lastname: Ma
mesh_terms: D026881:Acupuncture; D000818:Animals; D015671:Electroacupuncture; D007031:Hypothalamus / Q000378:metabolism*; D008159:Lumbar Vertebrae; D008297:Male; D011897:Random Allocation; D051381:Rats; D012844:Sincalide / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D013118:Spinal Cord Diseases / Q000378:metabolism* / Q000628:therapy; D013373:Substance P / Q000378:metabolism*
chemical_list: D013373:Substance P; D012844:Sincalide