title: Protection of chickens against avian influenza with non-replicating adenovirus-vectored vaccine.
abstract: Protective immunity against avian influenza (AI) virus was elicited in chickens by single-dose vaccination with a replication competent adenovirus (RCA)-free human adenovirus (Ad) vector encoding an H7 AI hemagglutinin (AdChNY94.H7). Chickens vaccinated in ovo with an Ad vector encoding an AI H5 (AdTW68.H5) previously described, which were subsequently vaccinated intramuscularly with AdChNY94.H7 post-hatch, responded with robust antibody titers against both the H5 and H7 AI proteins. Antibody responses to Ad vector in ovo vaccination follow a dose-response kinetic. The use of a synthetic AI H5 gene codon optimized to match the chicken cell tRNA pool was more potent than the cognate H5 gene. The use of Ad-vectored vaccines to increase resistance of chicken populations against multiple AI strains could reduce the risk of an avian-originating influenza pandemic in humans.
authors.affiliation: Department of Pathobiology, Auburn University, Auburn, AL 36849, USA. torohar@auburn.edu
authors.forename: Haroldo
authors.initials: H
authors.lastname: Toro
authors.forename: De-chu C
authors.initials: DC
authors.lastname: Tang
authors.forename: David L
authors.initials: DL
authors.lastname: Suarez
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D004306:Dose-Response Relationship, Immunologic; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control*; D010614:Pharynx / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; C109963:hemagglutinin, avian influenza A virus