A highly stable nonbiofouling surface with well-packed grafted zwitterionic polysulfobetaine for plasma protein repulsion.
An ideal nonbiofouling surface for biomedical applications requires both high-efficient antifouling characteristics in relation to biological components and long-term material stability from biological systems. In this study we demonstrate the performance and stability of an antifouling surface with grafted zwitterionic sulfobetaine methacrylate (SBMA). The SBMA was grafted from a bromide-covered gold surface via surface-initiated atom transfer radical polymerization to form well-packed polymer brushes. Plasma protein adsorption on poly(sulfobetaine methacrylate) (polySBMA) grafted surfaces was measured with a surface plasmon resonance sensor. It is revealed that an excellent stable nonbiofouling surface with grafted polySBMA can be performed with a cycling test of the adsorption of three model proteins in a wide range of various salt types, buffer compositions, solution pH levels, and temperatures. This work also demonstrates the adsorption of plasma proteins and the adhesion of platelets from human blood plasma on the polySBMA grafted surface. It was found that the polySBMA grafted surface effectively reduces the plasma protein adsorption from platelet-poor plasma solution to a level superior to that of adsorption on a surface terminated with tetra(ethylene glycol). The adhesion and activation of platelets from platelet-rich plasma solution were not observed on the polySBMA grafted surface. This work further concludes that a surface with good hemocompatibility can be achieved by the well-packed surface-grafted polySBMA brushes.
Chang
Yung
Y
R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Jhong-Li, Taoyuan 320, Taiwan.
Liao
Shih-Chieh
SC
Higuchi
Akon
A
Ruaan
Ruoh-Chyu
RC
Chu
Chih-Wei
CW
Chen
Wen-Yih
WY
D000327:Adsorption; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D001792:Blood Platelets / Q000378:metabolism; D001965:Bromides / Q000737:chemistry; D002448:Cell Adhesion; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008689:Methacrylates / Q000737:chemistry*; D008956:Models, Chemical; D010973:Platelet Adhesiveness; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
D001965:Bromides; D007477:Ions; D008689:Methacrylates; D011506:Proteins; D001622:Betaine; C483727:sulfobetaine; D005340:Fibrinogen