title: Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells.
abstract: To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.
authors.affiliation: Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Takano
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Nakazawa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okuno
authors.forename: Naritoshi
authors.initials: N
authors.lastname: Shirata
authors.forename: Sohken
authors.initials: S
authors.lastname: Tsuchiya
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kainoh
authors.forename: Seigoh
authors.initials: S
authors.lastname: Takamatsu
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Furuta
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Taketomi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Naito
authors.forename: Hiromu
authors.initials: H
authors.lastname: Takematsu
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kozutsumi
authors.forename: Gozoh
authors.initials: G
authors.lastname: Tsujimoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Murakami
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kudo
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ichikawa
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakayama
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Sugimoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D006632:Histamine / Q000032:analysis; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D010447:Peptide Hydrolases / Q000378:metabolism; D010537:Peritoneum / Q000166:cytology; D041701:Swiss 3T3 Cells
chemical_list: D006632:Histamine; D010447:Peptide Hydrolases