title: Fibrillin-1 in incisional hernias: an immunohistochemical study in scar and non-scar regions of human skin and muscle fasciae.
abstract: Incisional hernias represent one of the most common complications after laparotomy. Specific pre-operative risk factors have not yet been identified. Recent studies indicate that changes in extracellular matrix components such as collagen I and collagen III may be involved in hernia development. In the present study we have evaluated the significance of fibrillin-1 in hernia development as one of the main components of the extracellular matrix. Tissue samples from non-scar skin and muscle fascia of 12 patients with incisional hernias as well as from the respective scar tissues were obtained. Corresponding tissue samples of 10 patients with normal postoperative wound healing served as controls. Distribution of fibrillin-1 was evaluated immunohistochemically. Differences in fibrillin-1 distribution in the non-scar tissues of muscle fascia have been found in patients with incisional hernia, compared to those without hernia. In scar regions of both patient groups, slight differences in the pattern of fibrillin-1 were observed. A tendency to a differential deposition of fibrillin-1 in skin samples, although hardly quantifiable, was observed as well. Our results suggest that fibrillin-1 is a relevant factor contributing to tissue stability. Disturbances in its deposition, even before scar formation, may be an important factor to the development of incisional hernias.
authors.affiliation: Dept of Histology, University of Göttingen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Fricke
authors.forename: Claus
authors.initials: C
authors.lastname: Langer
authors.forename: Edgar
authors.initials: E
authors.lastname: Brunner
authors.forename: Lynn Y
authors.initials: LY
authors.lastname: Sakai
authors.forename: Laszlo
authors.initials: L
authors.lastname: Füzesi
authors.forename: Dieter P
authors.initials: DP
authors.lastname: Reinhardt
authors.forename: Fabio
authors.initials: F
authors.lastname: Quondamatteo
mesh_terms: D034861:Abdominal Wall; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002921:Cicatrix / Q000378:metabolism*; D024042:Collagen Type I / Q000032:analysis; D024061:Collagen Type III / Q000032:analysis; D005205:Fascia / Q000737:chemistry*; D005260:Female; D000071838:Fibrillin-1; D000071837:Fibrillins; D046449:Hernia, Abdominal / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007813:Laparotomy; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis*; D008875:Middle Aged; D011183:Postoperative Complications / Q000378:metabolism*; D012086:Reoperation; D012306:Risk; D012867:Skin / Q000737:chemistry*; D014945:Wound Healing; D054392:Adipokines
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D024061:Collagen Type III; D000071838:Fibrillin-1; D000071837:Fibrillins; D008840:Microfilament Proteins; C000605722:FBN1 protein, human; D054392:Adipokines