Enhancing sensitivity of liquid chromatographic/ion-trap tandem mass spectrometric determination of estrogens by on-line pre-column derivatization.
A rapid and sensitive liquid chromatographic/ion-trap mass spectrometric method has been developed for the simultaneous determination of estriol, alpha-, beta-estradiol, 17alpha-ethynylestradiol and estrone. The substances were extracted from river water samples (typically 250 ml) and cleaned-up using two-stage solid-phase extraction (SPE). 12-(Difluoro-1,3,5-triazinyl)-benz[f]isoindolo-[1,2b][1,3]benzothiazolidine was applied as a derivatizing agent. The recovery for each compounds ranged from 75 to 88% and the repeatability represented as RSDs ranged from 5.6 to 8.4%. Limits of detection (LOD, 3 x S/N) were 67-285 pg/l.
Matejícek
David
D
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno CZ 61300, Czech Republic.
Kubán
Vlastimil
V
D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000032:analysis*; D005618:Fresh Water; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
D004967:Estrogens; D007202:Indicators and Reagents; D014874:Water Pollutants, Chemical