Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.
The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.
Benson
Robert
R
CDC, Atlanta, GA 30333, USA.
Tondella
Maria L
ML
Bhatnagar
Julu
J
Carvalho
Maria da Glória S
Mda G
Sampson
Jacquelyn S
JS
Talkington
Deborah F
DF
Whitney
Anne M
AM
Mothershed
Elizabeth
E
McGee
Lesley
L
Carlone
George
G
McClee
Vondguraus
V
Guarner
Jeannette
J
Zaki
Sherif
S
Dejsiri
Surang
S
Cronin
K
K
Han
Jian
J
Fields
Barry S
BS
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic