TbG63, a golgin involved in Golgi architecture in Trypanosoma brucei.
Golgins are coiled-coil proteins that have been implicated in the structure and function of the Golgi complex. Here, we identify and characterize a trypanosomal golgin, TbG63, showing that it has a C-terminal membrane anchor and an N-terminus that projects into the cytoplasm. TbG63 in procyclic parasites is localized to the Golgi and interacts with the active, GTP-form of TbRab1A. Overexpression of TbG63 has dramatic effects on Golgi architecture -- effects that require the N-terminus -- whereas depletion has little, if any, effect on the growth rate. By contrast, in the bloodstream form of the parasite, depletion of TbG63 slows growth, although it has no obvious effect on the transport of a variant surface glycoprotein (VSG) or on Golgi structure. TbG63 might be a useful tool to study the structure and functioning of the Golgi complex.
Ramirez
Irene Barinaga-Rementeria
IB
Faculty of Life Sciences, University of Manchester, The Michael Smith Building, Manchester, UK.
de Graffenried
Christopher L
CL
Ebersberger
Ingo
I
Yelinek
Jordan
J
He
Cynthia Y
CY
Price
Albert
A
Warren
Graham
G
D000818:Animals; D019295:Computational Biology; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry; D010271:Parasites / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D014346:Trypanosoma brucei brucei / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000378:metabolism; D033921:Vesicular Transport Proteins; D020761:rab1 GTP-Binding Proteins / Q000378:metabolism
C060042:GOLGA5 protein, human; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D015800:Protozoan Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D033921:Vesicular Transport Proteins; D020761:rab1 GTP-Binding Proteins