title: [MLPA technique--principles and use in practice].
abstract: MLPA (Multiplex Ligation-dependent Probe Amplification) is a recently introduced method, based on PCR principle, useful for the detection of different genetic abnormalities (aneuploidies, gene deletions/duplications, subtelomeric rearrangements, methylation status etc). The technique is simple, reliable and cheap. We present this method to discuss its importance for a modern genetic service and to underline its multiple advantages.
authors.affiliation: Disciplina de Genetică Medicală, Facultatea de Medicină, Universitatea de Medicină si Farmacie "Gr.T. Popa", Iaşi.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rusu
authors.forename: Adriana
authors.initials: A
authors.lastname: Sireteanu
authors.forename: Maria
authors.initials: M
authors.lastname: Puiu
authors.forename: Cristina
authors.initials: C
authors.lastname: Skrypnyk
authors.forename: E
authors.initials: E
authors.lastname: Tomescu
authors.forename: Katalin
authors.initials: K
authors.lastname: Csep
authors.forename: Victoria
authors.initials: V
authors.lastname: Creţ
authors.forename: Ligia
authors.initials: L
authors.lastname: Barbarii
mesh_terms: D000782:Aneuploidy; D020732:Cytogenetic Analysis / Q000379:methods*; D019175:DNA Methylation; D017353:Gene Deletion; D020440:Gene Duplication; D015321:Gene Rearrangement; D005820:Genetic Testing; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results