Homogenization technique for analysis of post-iontophoretic acyclovir content in porcine skin.
The aim of present paper is to develop a method of analysis of acyclovir in porcine skin after iontophoretic delivery using a novel homogenization technique. There are various reported methods available for the analysis of acyclovir in skin samples. All of these methods involve assumption of complete absorption of spiked drug by skin slices for validation. In the current work this assumption has been removed. The method utilizes a step of homogenization to convert the skin into plasma-like matrix, which can be then conveniently used for validation of method as it is done for any plasma sample. It was found that this method can be conveniently used for the estimation of acyclovir in skin layers and that it also virtually removes the step of extraction.
Shaji
Jessy
J
Department of Pharmaceutics, Prin. K. M. Kundnani College of Pharmacy, Cuffe Parade, Colaba, Mumbai, India. jshaji@rediffmail.com
Marathe
Shripad
S
D000212:Acyclovir / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004058:Diffusion; D066298:In Vitro Techniques; D007478:Iontophoresis; D011786:Quality Control; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*; D013552:Swine
D000998:Antiviral Agents; D000212:Acyclovir