[Effects of ligfol after radiation-mercury exposure in rats of various age].
The study was carried out with the young (4-5 months) and elderly (15-18 months) female and male rats at the experiment start. The mercury salt was administered into animals with drinking water in 1 mcg/l concentration during 3 months before and 1 month after single X-irradiation with 25 cGy dose. The genotoxic effects in leukocytes were evaluated on the base of the content and structure DNA alterations in 24 hrs and in 1 mth after irradiation. The alaninaminotransferase activity in serum was determined to evaluate functional status of liver. A protector intramuscular injection of Ligfol preparation with the 250 mg/kg dose in 24 hrs before irradiation resulted in increase of the blood leukocyte amount, more expressed in 1 mth after irradiation. The standardizing effect of preparation on the leukocyte DNA content is manifested in 1 mth after combined exposure on rats within young male and elderly female. At the same time, Ligfol showed some restoring effect on the alterations of leukocyte supercoiled DNA structure in 1 mth after irradiation within elderly female rats. The preparation showed the standardizing effect on the liver cells in young males injured by radiation-mercury exposures with low doses, however Ligfol had not influence practically on the destruction degree of hepatocytes after similar injury within female young and elderly rats. So, results of the intramuscular Ligfol injection is manifested at least during 1-2 mths; the young rats of both gender and elderly females showed the most sensitivity to the radiation-mercury exposure with low doses and to the Ligfol, according to the studied indexes.
Ivanov
S D
SD
Sobutskiĭ
M P
MP
Kovan'ko
E G
EG
Liutinskiĭ
S I
SI
Berkovich
A M
AM
D000375:Aging / Q000097:blood / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D004249:DNA Damage; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008031:Lignin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D008111:Liver Function Tests; D008297:Male; D017669:Mercury Compounds / Q000633:toxicity*; D009566:Nitrates / Q000633:toxicity*; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000209:etiology / Q000517:prevention & control; D051381:Rats
D000975:Antioxidants; D017669:Mercury Compounds; D009566:Nitrates; C413426:olipifat; C029948:mercury nitrate; D008031:Lignin