Major anthocyanins from purple asparagus (Asparagus officinalis).
Two major anthocyanins (A1 and A2) were isolated from peels of the spears of Asparagus officinalis cv. Purple Passion. They were purified by column, paper and high-performance liquid chromatographic separations, and their structures were elucidated by high-resolution Fourier transform ion cyclotron resonance mass spectrometry (HR-FT-ICR MS), 1H, 13C and two-dimensional NMR spectroscopic analyses and either acid or alkaline hydrolysis, respectively. A1 was identified as cyanidin 3-[3''-(O-beta-d-glucopyranosyl)-6''-(O-alpha-l-rhamnopyranosyl)-O-beta-d-glucopyranoside], whereas A2 was cyanidin 3-rutinoside, which is widely distributed in higher plants. Oxygen radical absorbance capacity (ORAC) assays proved their high antioxidant activities.
Sakaguchi
Yumi
Y
Laboratory of Horticultural Science, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-Ku, Fukuoka 812-8581, Japan. yumi_s@agr.kyushu-u.ac.jp
Ozaki
Yukio
Y
Miyajima
Ikuo
I
Yamaguchi
Masaatsu
M
Fukui
Yuko
Y
Iwasa
Keiko
K
Motoki
Satoru
S
Suzuki
Takashi
T
Okubo
Hiroshi
H
D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D027761:Asparagus Plant / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry
D000872:Anthocyanins; D000975:Antioxidants