SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis.
Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden. The expression profile was obtained from serial analysis of gene expression (SAGE) library data produced from 3- and 6-year-old trees. Fourteen-base expressed sequence tags (ESTs) were searched against public Eucalyptus ESTs and annotated with GenBank. Altogether 43,304 tags were generated producing 3066 unigenes with three or more copies each, 445 with a putative identity, 215 with unknown function and 2406 without an EST match. The expression profile of the juvenile cambial region revealed the presence of highly frequent transcripts related to general metabolism and energy metabolism, cellular processes, transport, structural components and information pathways. We made a quantitative analysis of a large number of genes involved in the biosynthesis of cellulose, pectin, hemicellulose and lignin. Our findings provide insight into the expression of functionally related genes involved in juvenile wood formation in young fast-growing E. grandis trees.
Gallo de Carvalho
Mayra Costa da Cruz
MC
Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba-SP, Brazil.
Caldas
Danielle Gregorio Gomes
DG
Carneiro
Raphael Tozelli
RT
Moon
David Henry
DH
Salvatierra
Guillermo Rafael
GR
Franceschini
Lívia Maria
LM
de Andrade
Alexander
A
Celedon
Paola Alejandra Fiorani
PA
Oda
Shinitiro
S
Labate
Carlos Alberto
CA
D000064:Acclimatization; D003001:Cloning, Molecular; D017931:DNA Primers; D004798:Enzymes / Q000235:genetics; D005052:Eucalyptus / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D015723:Gene Library; D017343:Genes, Plant; D006019:Glycolysis; D010940:Plant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers; D004798:Enzymes; D010940:Plant Proteins