Development and validation of high performance liquid chromatography method for analysis of sibutramine hydrochloride and its impurity.
A simple, Precise, Rapid reproducible and selective reverse phase HPLC method has been developed for the estimation of Sibutramine Hydrochloride monohydrate and its Impurity in Bulk as well as Formulation. The analyte was resolved by using Mobile phase (Sodium Dihydrogen phosphate and Acetonitrile) at the flow rate of 1.0 Ml/Min. on Isocratic HPLC system consisting of Jasco Make UV visible Detector of model UV 1575 & Jasco make HPLC pump of model PU 1580. An ODS C- 8 RP Column (4.6mm ID, 250mm L, particle size 5 Micron, at wavelength of 230 nm.
Chandorkar
J G
JG
Aarti Drugs Ltd., Mumbai, India. jgchandorkar@innovassynth.com
Kotwal
V B
VB
Dhande
N S
NS
Pachpor
M P
MP
Pande
V V
VV
D000928:Antidepressive Agents / Q000737:chemistry / Q000302:isolation & purification; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003503:Cyclobutanes / Q000737:chemistry* / Q000302:isolation & purification; D004364:Pharmaceutical Preparations / Q000032:analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000928:Antidepressive Agents; D003503:Cyclobutanes; D004364:Pharmaceutical Preparations; C058254:sibutramine