title: Cultured keratinocytes in fibrin with decellularised dermis close porcine full-thickness wounds in a single step.
abstract: The purpose of this study was to assess the feasibility of combined keratinocyte and dermal scaffold transplantation performed in a single step for treatment of full-thickness wounds. Cultured autologous keratinocytes were suspended in fibrin and grafted together with decellularised human dermis (Alloderm) in a porcine animal model, involving 10 animals over 4 weeks. Wound healing was evaluated by planimetry. Histology included morphological analysis as well as immunohistochemistry at regular intervals (1, 2 and 4 weeks). The results showed both successful histo-integration of the in vivo composite grafts and reduced wound contraction, compared with the control group (plain epithelial grafts). Histologically a neo-epithelium originated from the grafted cells on top of the decellularised dermis, as well as a reconstituted basement membrane. After 4 weeks cellular ingrowth into the dermal matrix could be observed. The successful combination of a keratinocyte-fibrin suspension and acellular dermis applied in a single step onto full-thickness wounds resulted in closure.
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Str. 55, D-79106 Freiburg, Germany. holger.bannasch@uniklinik-freiburg.de
authors.forename: Holger
authors.initials: H
authors.lastname: Bannasch
authors.forename: Tim
authors.initials: T
authors.lastname: Unterberg
authors.forename: Matthias
authors.initials: M
authors.lastname: Föhn
authors.forename: Birgit
authors.initials: B
authors.lastname: Weyand
authors.forename: Raymund E
authors.initials: RE
authors.lastname: Horch
authors.forename: G Björn
authors.initials: GB
authors.lastname: Stark
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D005260:Female; D005337:Fibrin / Q000627:therapeutic use*; D007091:Image Processing, Computer-Assisted; D015603:Keratinocytes / Q000473:pathology / Q000637:transplantation*; D023421:Models, Animal; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013552:Swine; D014182:Transplantation, Autologous; D014945:Wound Healing
chemical_list: C440568:Alloderm; D005337:Fibrin; D003094:Collagen