title: Stopped-flow kinetic fluorimetric studies of the interaction of Ru(II) complex with DNA and its analytical application.
abstract: A simple, rapid, and sensitive stopped-flow kinetic fluorometric approach was established for the assay of DNA in synthetic samples and real samples by using the measures of initial reaction rate. The increased initial reaction rate is in proportion to the concentration of DNA in the range of 2.0x10(-8)M to 2.1x10(-6)M. The optimum conditions for various parameters on which the binding of Ru(phen)(2)(dppz)(2+) to DNA depends, were investigated. The influence of various surfactants on the interaction was discussed. Furthermore, stopped-flow techniques were employed to determine kinetic parameters of Ru(phen)(2)(dppz)(2+) binding to DNA under pseudo-first-order conditions. It was found that the interaction of Ru(phen)(2)(dppz)(2+) with DNA was very fast. A two-step reaction mechanism, a fast phase followed by a slow phase, was proposed.
authors.affiliation: Department of Chemistry, Zhengzhou University, Zhengzhou 450001, China. rywang97@163.com
authors.forename: Rui-Yong
authors.initials: RY
authors.lastname: Wang
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Ji
authors.forename: Ruiqiang
authors.initials: R
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Shi
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005470:Fluorometry / Q000379:methods / Q000706:statistics & numerical data; D007477:Ions; D007700:Kinetics; D009942:Organometallic Compounds / Q000737:chemistry*; D055668:Photochemical Processes; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000706:statistics & numerical data; D013501:Surface-Active Agents
chemical_list: D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D007477:Ions; D009942:Organometallic Compounds; C466600:Ru(phen)(2)dipyrido(3,2-a-2',3'-c)phenazine; D013501:Surface-Active Agents; D012428:Ruthenium; D004247:DNA