title: [Biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts].
abstract: OBJECTIVE
  To investigate biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts (HPDLF) for verifying the hypothesis of delivering medicine to the periodontium and whole body through the root canal.
  
  
  METHODS
  HPDLF and MC3T3-E1 cells were incubated in antibiotics solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by bradford protein assay.
  
  
  RESULTS
  The intracellular contents increased with incubation time. The extracellular medicine concentration had effect on the intracellular contents.
  
  
  CONCLUSIONS
  Tetracycline hydrochloride can be transported into HPDLF with incubation and this transport is time-dependent and concentration-dependent.
authors.affiliation: Dental Institute, General Hospital of PLA, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: E
authors.forename: Bin
authors.initials: B
authors.lastname: Leng
mesh_terms: D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D001692:Biological Transport; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D010513:Periodontal Ligament / Q000378:metabolism*; D013752:Tetracycline / Q000493:pharmacokinetics*
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline