[Stable expression of promoterless bar gene in transgenic rape plants].
Phosphinothricin resistant plants of two rapeseed (Brassica napus L. var. oleifera DC.) spring industrial cultivars were obtained by Agrobacterium tumefaciens leaf disk transformation. Vector constructions contained the promoterless coding sequence of phosphinothricin acetyltransferase (bar) gene located between two inverted lox-sites (elements of Cre/lox recombination system of P1 phage) and selective neomycinphosphotransferase II gene (nptII). Integration of the alien genes was confirmed by the PCR analyses. Stable and linked inheritance of foreign genes in T1 and T2 progeny was shown.
Sakhno
L A
LA
Gocheva
E A
EA
Komarnitskiĭ
I K
IK
Kuchuk
N V
NV
D000123:Acetyltransferases / Q000235:genetics*; D016960:Agrobacterium tumefaciens / Q000472:pathogenicity; D000613:Aminobutyrates / Q000494:pharmacology; D029688:Brassica napus / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D015870:Gene Expression; D017343:Genes, Plant; D005822:Genetic Vectors; D053209:Herbicide Resistance / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000382:microbiology; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic
D000613:Aminobutyrates; C003121:phosphinothricin; D000123:Acetyltransferases; C056243:phosphinothricin N-acetyltransferase