title: Efficient in vivo electroporation of the postnatal rodent forebrain.
abstract: Functional gene analysis in vivo represents still a major challenge in biomedical research. Here we present a new method for the efficient introduction of nucleic acids into the postnatal mouse forebrain. We show that intraventricular injection of DNA followed by electroporation induces strong expression of transgenes in radial glia, neuronal precursors and neurons of the olfactory system. We present two proof-of-principle experiments to validate our approach. First, we show that expression of a human isoform of the neural cell adhesion molecule (hNCAM-140) in radial glia cells induces their differentiation into cells showing a neural precursor phenotype. Second, we demonstrate that p21 acts as a cell cycle inhibitor for postnatal neural stem cells. This approach will represent an important tool for future studies of postnatal neurogenesis and of neural development in general.
authors.affiliation: IBDML, CNRS/Université de Méditerranée, Campus de Luminy, Marseille, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Boutin
authors.forename: Simone
authors.initials: S
authors.lastname: Diestel
authors.forename: Angélique
authors.initials: A
authors.lastname: Desoeuvre
authors.forename: Marie-Catherine
authors.initials: MC
authors.lastname: Tiveron
authors.forename: Harold
authors.initials: H
authors.lastname: Cremer
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004247:DNA / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D020869:Gene Expression Profiling; D005821:Genetic Techniques; D008954:Models, Biological; D009457:Neuroglia / Q000166:cytology; D009830:Olfactory Bulb / Q000378:metabolism; D010641:Phenotype; D016548:Prosencephalon / Q000254:growth & development / Q000378:metabolism / Q000473:pathology*; D020033:Protein Isoforms; D051381:Rats; D019076:Transgenes
chemical_list: D020033:Protein Isoforms; D004247:DNA