title: Safety evaluation of pulmonary influenza vaccination in healthy and "asthmatic" mice.
abstract: The present study reports animal immuno-toxicological data of pulmonary vaccination against inactivated seasonal influenza. Its aims were (i) to monitor the temporal kinetics of lung inflammation in normal mice over a period of 2 weeks following pulmonary vaccination in order to assess the risk of chronic lung inflammation, (ii) to evaluate the impact of pulmonary vaccination on the asthmatic phenotype in an established allergen-sensitized murine model of asthma. Both sets of experiments were performed using high doses of split influenza virus vaccine. In the first part of this study, we showed that pulmonary influenza vaccination induced a slight local inflammatory response which was limited in duration since it was no longer observed at 2 weeks post-vaccination. At this time point, it has previously been shown that the immunogenic efficacy was maintained. In the second part, we demonstrated that pulmonary influenza vaccination did not significantly exacerbate the cardinal features of asthma, i.e., allergen-specific IgE formation, the development of airway hyperreactivity (AHR) and eosinophilic airway inflammation. Our data therefore suggest that the overall immuno-toxicological profile of pulmonary vaccination against seasonal influenza was acceptable, even in an animal model of pulmonary hypersensitivity.
authors.affiliation: Department of Pharmaceutical Technology, Université catholique de Louvain, Avenue E. Mounier, 73 UCL 73.20, Brussels B-1200, Belgium.
authors.forename: Antoine
authors.initials: A
authors.lastname: Minne
authors.forename: François
authors.initials: F
authors.lastname: Huaux
authors.forename: Joanna
authors.initials: J
authors.lastname: Jaworska
authors.forename: Ro-Dug
authors.initials: RD
authors.lastname: Rha
authors.forename: Eckard
authors.initials: E
authors.lastname: Hamelmann
authors.forename: Rita
authors.initials: R
authors.lastname: Vanbever
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D005260:Female; D007249:Inflammation; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000009:adverse effects*; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects
chemical_list: D007252:Influenza Vaccines; D014409:Tumor Necrosis Factor-alpha; D015164:Vaccines, Inactivated