Molybdate transport through the plant sulfate transporter SHST1.
Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1, on media containing low amounts of molybdate. Kinetic analysis using 99MoO4(2-) demonstrated that SHST1 enhanced the uptake of molybdate into yeast cells at nM concentrations. Uptake was not inhibited by sulfate, but sulfate transport via SHST1 was reduced with molybdate. These results are the first measurement of molybdate transport by a characterised plant sulfate transport protein.
Fitzpatrick
Kate L
KL
School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus Urrbrae, SA 5064, Australia.
Tyerman
Stephen D
SD
Kaiser
Brent N
BN
D007887:Fabaceae / Q000378:metabolism*; D005816:Genetic Complementation Test; D017136:Ion Transport; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008982:Molybdenum / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*
D026901:Membrane Transport Proteins; D010940:Plant Proteins; D013431:Sulfates; C044659:molybdate; D008982:Molybdenum