Relative quantification of canine CD56 mRNA expression by real-time polymerase chain reaction method in normal tissues and activated lymphocytes.
Real-time PCR was optimized for the quantification of canine CD56 mRNA expression. This study was conducted to easily quantify canine CD56 expression and to identify its expression in normal tissues, peripheral blood mononuclear cells and activated lymphocytes in dogs. This assay revealed the highest level of CD56 mRNA expression in the normal canine brain, followed by the lung, kidney and liver. CD56 mRNA expression level in peripheral blood mononuclear cells was considerably lower; among activated lymphocytes in vitro, CD56 mRNA expression was increased.
Hoshino
Yuki
Y
Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, Japan. yukito@vetmed.hokudai.ac.jp
Tajima
Motoshi
M
Takagi
Satoshi
S
Osaki
Tomohiro
T
Okumura
Masahiro
M
Fujinaga
Toru
T
D000818:Animals; D019002:CD56 Antigen / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004285:Dogs / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000378:metabolism*
D019002:CD56 Antigen; D017931:DNA Primers; D012333:RNA, Messenger