Abnormal transforming growth factor-beta expression in mesenchymal stem cells from patients with osteoarthritis.
OBJECTIVE
To determine the expression of the genes that code for the isoforms of transforming growth factor-beta (TGF-beta) and TGF-beta receptors (TBR) in mesenchymal stem cells (MSC) from patients with osteoarthritis (OA).


METHODS
Total RNA was extracted from primary cultures of MSC and quantitative real-time reverse transcription-polymerase chain reaction was performed to analyze gene expression.


RESULTS
MSC from patients with OA showed significantly increased total TGF-beta, TGF-beta1 isoform, TBR-II, and TBR-III mRNA expression compared to controls.


CONCLUSIONS
Our study is the first reporting the gene expression levels of TGF-beta and its isoforms and receptors in patients with OA. These findings might have pathological significance for OA disease.
Rollín
Raquel
R
Servicio de Cirugía Ortopédica y Traumatología, Hospital Clínico San Carlos, Madrid, Spain.
Alvarez-Lafuente
Roberto
R
Marco
Fernando
F
García-Asenjo
Jose Antonio
JA
Jover
Juan Angel
JA
Rodríguez
Luis
L
López-Durán
Luis
L
Fernández-Gutiérrez
Benjamín
B
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D053781:Transforming Growth Factor beta2 / Q000378:metabolism; D053782:Transforming Growth Factor beta3 / Q000378:metabolism
D020033:Protein Isoforms; D011509:Proteoglycans; D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D053781:Transforming Growth Factor beta2; D053782:Transforming Growth Factor beta3; C071736:betaglycan; D017346:Protein Serine-Threonine Kinases; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II