Evaluation of growth inhibitory and apoptosis inducing activity of human calprotectin on the human gastric cell line (AGS).
Calprotectin is cytotoxic agent that its anti-tumor effects are governed through suppression of topoisomerase II; a key enzyme in apoptosis. In previous studies, cytotoxicity and apoptotic effects of calprotectin are shown on different cancer cell lines, but not human gastric cancer cell lines. In the present study, cytotoxicity and apoptotic effects of calprotectin on human gastric adenocarcinoma cancer cell line (AGS) were evaluated.



The AGS cells were exposed to the different concentrations of calprotectin for 24, 48 and 72 hours. Cell proliferation was assessed using dimethylthiazol diphenyl tetrazolium bromide assay and dye exclusion tests. For evaluation of cytotoxic mechanism in calprotectin on AGS cells, flow cytometric analysis was performed.



Our results revealed that calprotectin induces growth inhibition of AGS in a dose- and time-dependent manner. Results of this investigation showed that sensitivity of AGS cells to cytotoxic effect of human calprotectin was highly remarkable. In addition, growth inhibitory effect of this cytotoxic agent mostly was governed through induction of apoptosis in the AGS cells.



These findings indicated that calprotectin induces growth inhibition and apoptosis in the AGS cells.
Zali
Hakimeh
H
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.|Dept. of Mole3Medicine Faculty, Ilam Medical Science University, Ilam; cular Biology, Khatam University, Tehran, Iran.
Rezaei-Tavirani
Mostafa
M
Dept. of Mole3Medicine Faculty, Ilam Medical Science University, Ilam; cular Biology, Khatam University, Tehran, Iran.|Medicine Faculty, Ilam Medical Science University, Ilam, Tehran, Iran.|4Asre-Novin Institute of Research and Services, Unit 15, Nariman Building, Kamranyeh Junction, Shaheed-Bahonar Ave., Tehran, Iran.
Kariminia
Amina
A
Dept.  of Immunology, Pasteur Institute of Iran, Tehran, Iran.
Yousefi
Reza
R
Institute of Biochemistry and Biophysics, Tehran, Iran
Shokrgozar
Mohammad Ali
MA
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D039841:Leukocyte L1 Antigen Complex / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology
D017304:Annexin A5; D039841:Leukocyte L1 Antigen Complex