DNMT3B 579 G>T promoter polymorphism and risk of esophagus carcinoma in Chinese.
OBJECTIVE
To investigate the relationship between 579 G>T polymorphisms in the DNMT3B gene, which is involved in de novo methylation and associated with the risk of esophagus cancer (EC) in Chinese.


METHODS
DNMT3B 579 G>T genotypes were determined by PCR-RFLP in 194 EC patients and 210 healthy controls matched for age and sex, who did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed EC.


RESULTS
In control subjects, the frequency of T/T and G/T genotypes, and T and G alleles was 81.4%, 18.1%, 90.05% and 9.55%, respectively. The distribution of genotypes and allelotypes in the EC patients was not significantly different from that in the controls. When stratified by sex and age, there was still no significant association between the risks of EC and GT and GG genotypes. This study also showed a distinct difference in the distribution of DNMT3B and single nucleotide polymorphism (SNP) between Chinese and Koreans.


CONCLUSIONS
DNMT3B 579 G>T polymorphism may not be a stratification marker to predict the susceptibility to EC, at least in Chinese. DNMT3B promoter SNP is diverse in ethnic populations.
Fan
Hong
H
Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China. fanh@seu.edu.cn
Liu
Dong-Sheng
DS
Zhang
Shu-Hong
SH
Hu
Jia-Bo
JB
Zhang
Feng
F
Zhao
Zhu-Jiang
ZJ
D000483:Alleles; D001483:Base Sequence; D002277:Carcinoma / Q000175:diagnosis* / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000502:physiology*; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D000097584:DNA Methyltransferase 3B
D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B