Interaction behaviors between chitosan and hemoglobin.
The interaction and association between chitosan and hemoglobin (Hb) are studied by UV-vis and fluorescence spectroscopies, viscometry, circular dichroism, dynamic and static light scattering. Chitosan can obviously associate with Hb to form protein-chitosan complexes, which affects microstructure of Hb. The distance between the first association site of chitosan with 214-tryptophan residue in Hb is about 5.473 nm. The intrinsic UV-vis absorption and fluorescence intensities of Hb increase with an increase of chitosan concentration. The alpha-helix in Hb is drawn and changed into beta-sheet.
Chen
Liuhua
L
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jinagsu, 25002, PR China.
Tianqing
Liu
L
D002240:Carbohydrate Sequence; D048271:Chitosan / Q000737:chemistry*; D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013053:Spectrophotometry; D014783:Viscosity; D014867:Water / Q000737:chemistry
D006454:Hemoglobins; D014867:Water; D048271:Chitosan