Development of a one-step strip for the detection of triazophos residues in environmental samples.
Environmental and food safety issues now are recognized internationally, and pesticide residues play key roles as environment and food pollutants. It is crucial to develop methods for rapid determination of pesticide residues in environments and foods. A one-step strip based on nanocolloidal-gold-labeled monoclonal antibodies for detection of triazophos residue was developed. The nanocolloidal gold, with an average particle diameter of 25 nm (G25), was labeled to an antitriazophos monoclonal antibody. This conjugate was dispensed on the conjugate pad of a porous glass fiber. Ovalbumin hapten and goat anti-mouse IgG were dispensed on the nitrocellulose membrane and served as the test line (T-line) and control line (C-line), respectively. After conditions optimization, the one-step strip was finally developed for the residue determination of triazophos. The limit of detection (LOD) of the strip was 4 ng/mL for standard. The detection was not affected by the pH of the liquid sample but low total ion concentration will induce illegible C-line and T-line. The LOD for spiked samples of soil and water was 5ng/mL, with run time of no more than 10min.
Gui
Wen-Jun
WJ
Institute of Pesticide and Environmental Toxicology, Zhejiang University, 268 kaixuan road, Hangzhou 310029, China.
Wang
Shu-Ting
ST
Guo
Yi-Rong
YR
Zhu
Guo-Nian
GN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003101:Collodion / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D053768:Metal Nanoparticles / Q000737:chemistry; D063086:Organothiophosphates / Q000032:analysis* / Q000276:immunology; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D012987:Soil / Q000032:analysis; D014230:Triazoles / Q000032:analysis* / Q000276:immunology; D014867:Water / Q000737:chemistry
D000911:Antibodies, Monoclonal; D003102:Colloids; D004785:Environmental Pollutants; D007074:Immunoglobulin G; D063086:Organothiophosphates; D010573:Pesticide Residues; D012987:Soil; D014230:Triazoles; D014867:Water; C012584:triazophos; D006046:Gold; D003101:Collodion