Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia.
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.
Hou
Xiao-li
XL
Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Jiang
Han-liang
HL
Cao
Qing-yi
QY
Zhao
Li-ying
LY
Chang
Barbara J
BJ
Chen
Zhi
Z
D016470:Bacteremia / Q000175:diagnosis* / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D015342:DNA Probes; D005821:Genetic Techniques; D008089:Listeria monocytogenes / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009841:Oligonucleotides / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000737:chemistry; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013234:Stem Cells
D015342:DNA Probes; D009841:Oligonucleotides; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S