Skin decontamination of glyphosate from human skin in vitro.
This study compared three model decontaminant solutions (tap water, isotonic saline, and hypertonic saline) for their ability to remove a model herbicide (glyphosate) from an in vitro human skin model. Human cadaver skin was dosed (approximately 375microg) of [14C]-glyphosate on 3cm2 per skin. After each exposure time (1, 3, and 30min post-dosing, respectively), the surface skin was washed three times (4ml per time) with each solution. After washing, the skin was stripped twice with tape discs. Lastly, the wash solutions, strippings, receptor fluid, and remainder of skin were liquid scintillation analyzer counted to determine the amount of glyphosate. There were no statistical differences among these groups at any time points. The total mass balance recovery at three time exposure points was between 94.8% and 102.4%. The wash off rates (glyphosate in wash solutions) at three different exposure times is 79-101.2%. Thus the three tested decontaminants possess similar effectiveness in removing glyphosate from skin. This in vitro model is not only economic and rapid, but also provides quantitative data that may aid screening for optimal decontaminants.
Zhai
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Department of Dermatology, University of California, School of Medicine, Box 0989, Surge 110, 90 Medical Center Way, San Francisco, CA 94143-0989, USA. zhaih@derm.ucsf.edu
Chan
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HP
Hui
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Maibach
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D003666:Decontamination / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D006540:Herbicides / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D009997:Osmotic Pressure; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D012869:Skin Absorption; D000097797:Glyphosate
D005998:Glycine; D006540:Herbicides; D006982:Hypertonic Solutions; D000097797:Glyphosate