title: [Evaluation of the cumulative effect of meglumine antimoniate on the progeny of swiss mice: biologic assay].
abstract: OBJECTIVE
  Evaluate the effect of Meglumine Antimoniate on maternal-fetal transference in F1 generations (offspring of dams exposed to the drug), and embryotoxicity in F2 generations.
  
  
  METHODS
  Female Swiss mice were treated with daily s.c. injection of Meglumine Antimoniate (100mgSb v/kg bw/day) from day 7 until day 12 of pregnancy. The control group received only the vehicle (distilled water). After birth of offspring (F1 generation), 59 females were examined daily for determination of the estral cycle. When the cycle estrus was determined, males were mated with 18 females of the same lineage. On day 18 of pregnancy, females were euthanasied in a chamber of CO2 and after incision of the abdomen, the uterus was exposed. Then, resorptions as well as living and dead fetuses were evaluated, also the number of embryo/fetal implantation sites. Fetuses and their placenta were weighted to calculate the placental index. Three placentas of each litter were separated for microscopic analysis.
  
  
  RESULTS
  Administration of the Meglumine Antimoniate did not interfere in the estral cycle of the treated group, since it did not alter the precoital interval and fertility index. Placenta alterations were not observed in the F2 generations.
  
  
  CONCLUSIONS
  Meglumine Antimoniate did not interfere in the reproductive performance, after chronic exposition of dams. Data suggest that there is a gradual elimination of Meglumine Antimoniate by the maternal organism without damaging the future offspring.
authors.affiliation: Universidade Regional Integrada do Alto Uruguai e das Missões, Campus de Erechim, Erechim, RS, Brazil.
authors.forename: Michelle Rodrigues Dos
authors.initials: MR
authors.lastname: Santos
authors.forename: Clarisse Juliana
authors.initials: CJ
authors.lastname: Krignl
authors.forename: Alexandra
authors.initials: A
authors.lastname: Nava
authors.forename: Camila Michele Sabedot
authors.initials: CM
authors.lastname: Reik
authors.forename: Fabiana Ernestina Barcellos da
authors.initials: FE
authors.lastname: Silva
authors.forename: Silvane Souza
authors.initials: SS
authors.lastname: Roman
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000633:toxicity*; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D047109:Fetal Development / Q000187:drug effects*; D008431:Maternal-Fetal Exchange; D008536:Meglumine / Q000008:administration & dosage / Q000633:toxicity*; D000077485:Meglumine Antimoniate; D051379:Mice; D023421:Models, Animal; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000633:toxicity*; D010920:Placenta / Q000187:drug effects / Q000473:pathology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012098:Reproduction / Q000187:drug effects
chemical_list: D000981:Antiprotozoal Agents; D009942:Organometallic Compounds; D008536:Meglumine; D000077485:Meglumine Antimoniate