title: [Isolation of hepatitis E virus from liver of swine at abattoirs in Shandong province].
abstract: OBJECTIVE
  To determine the prevalence and genotype of hepatitis E virus (HEV) in swine liver before on the market and to analyze the phylogenetic relationship between the isolates from swine and human.
  
  
  METHODS
  35 swine liver specimens were collected from two slaughtering houses in the countryside of Shandong province, China. Nested reverse transcription-polymerase chain reaction (nested RT-PCR) and subsequent sequencing were used to determine the nucleotide sequences. A phylogenetic tree was constructed with Neighbor-joining method based on the Kimura-2-parameter model.
  
  
  RESULTS
  3. (8.57%) of the 35 swine liver specimens being tested were positive for HEV RNA. The three swine HEV strains isolated in the present study from liver samples shared the highest identity to genotype-IV HEV.
  
  
  CONCLUSIONS
  Results from the study confirmed that HEV was detectable among swine before on the market and the genotype was the same as that representing human and swine isolates in China. It also suggested that much more attention should be paid to the safety on the digestion of swine liver.
authors.affiliation: Department of Epidemiology, School of Public Health, Key Laboratory on Public Health Safety, Ministry of Education, Fudan University, Shanghai, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Yi-han
authors.initials: YH
authors.lastname: Lu
authors.forename: Ying-jie
authors.initials: YJ
authors.lastname: Zheng
authors.forename: Qing-wu
authors.initials: QW
authors.lastname: Jiang
mesh_terms: D000003:Abattoirs; D000818:Animals; D002681:China / Q000453:epidemiology; D005838:Genotype; D016751:Hepatitis E / Q000453:epidemiology / Q000821:virology; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D008099:Liver / Q000821:virology*; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013552:Swine / Q000821:virology*
chemical_list: D012367:RNA, Viral