title: Environmental adaptation: genomic analysis of the piezotolerant and psychrotolerant deep-sea iron reducing bacterium Shewanella piezotolerans WP3.
abstract: Shewanella species are widespread in various environments. Here, the genome sequence of Shewanella piezotolerans WP3, a piezotolerant and psychrotolerant iron reducing bacterium from deep-sea sediment was determined with related functional analysis to study its environmental adaptation mechanisms. The genome of WP3 consists of 5,396,476 base pairs (bp) with 4,944 open reading frames (ORFs). It possesses numerous genes or gene clusters which help it to cope with extreme living conditions such as genes for two sets of flagellum systems, structural RNA modification, eicosapentaenoic acid (EPA) biosynthesis and osmolyte transport and synthesis. And WP3 contains 55 open reading frames encoding putative c-type cytochromes which are substantial to its wide environmental adaptation ability. The mtr-omc gene cluster involved in the insoluble metal reduction in the Shewanella genus was identified and compared. The two sets of flagellum systems were found to be differentially regulated under low temperature and high pressure; the lateral flagellum system was found essential for its motility and living at low temperature.
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen, People's Republic of China.
authors.forename: Fengping
authors.initials: F
authors.lastname: Wang
authors.forename: Jianbin
authors.initials: J
authors.lastname: Wang
authors.forename: Huahua
authors.initials: H
authors.lastname: Jian
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Shengkang
authors.initials: S
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zeng
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.forename: Douglas Hoyt
authors.initials: DH
authors.lastname: Bartlett
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Songnian
authors.initials: S
authors.lastname: Hu
authors.forename: Xiang
authors.initials: X
authors.lastname: Xiao
mesh_terms: D002465:Cell Movement; D003580:Cytochromes / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D004777:Environment; D015964:Gene Expression Regulation, Bacterial; D005821:Genetic Techniques; D023281:Genomics; D007501:Iron / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D040901:Proteomics / Q000379:methods; D012623:Seawater; D020592:Shewanella / Q000378:metabolism*
chemical_list: D003580:Cytochromes; D015118:Eicosapentaenoic Acid; D007501:Iron