Thin semitransparent gels containing phenylboronic acid: porosity, optical response and permeability for sugars.
Radical copolymerization of acrylamide (Am) (90 mol%) with N-acryloyl-m-aminophenylboronic acid (NAAPBA) (10 mol%) carried out on the surface of glass slides in aqueous solution and in the absence of chemical cross-linkers, resulted in the formation of thin semitransparent gels. The phenylboronic acid (PBA) ligand density was ca. 160 micromol/ml gel. The gels exhibited a macroporous structure and displayed optical response to sucrose, lactose, glucose and fructose in 50 mM sodium phosphate buffer, in the pH range from 6.5 to 7.5. The response was fairly reversible and linearly depended on glucose concentration in the wide concentration range from 1 to 60 mM at pH 7.3. The character of response was explained by the balance of two competing equilibrium processes: binding of glucose to phenylboronate anions and binary hydrophobic interactions of neutral PBA groups. The apparent diffusion coefficient of glucose in the gels was ca. 2.5 x 10(-7) cm(2)/s. A freshly prepared gel can be used daily for at least 1 month without changes in sensitivity. Autoclaving (121 degrees C, 1.2 bar, 10 min) allows for the gels sterilization, which is important for their use as glucose sensors in fermentation processes.
Ivanov
Alexander E
AE
Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-22100 Lund, Sweden. alexander.ivanov@biotek.lu.se
Thammakhet
Chongdee
C
Kuzimenkova
Marina V
MV
Thavarungkul
Panote
P
Kanatharana
Proespichaya
P
Mikhalovska
Lyubov I
LI
Mikhalovsky
Sergey V
SV
Galaev
Igor Yu
IY
Mattiasson
Bo
B
D001897:Boronic Acids / Q000737:chemistry*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D004058:Diffusion / Q000187:drug effects; D005782:Gels; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D008854:Microscopy, Electron; D055095:Optics and Photonics; D010539:Permeability / Q000187:drug effects; D016062:Porosity / Q000187:drug effects
D001897:Boronic Acids; D005782:Gels; D005947:Glucose; C010686:benzeneboronic acid