title: Quantitative evaluation of signaling events in Drosophila S2 cells.
abstract: Drosophila activates a robust defense response to gram-negative bacteria through the Immune deficiency (Imd) pathway. Imd signaling proceeds through c-Jun N-terminal Kinase (JNK), NF-kB and caspase modules. The individual signaling modules act in a highly coordinated manner to yield a stereotypical response to infection. While considerable attention has focused on NF-kB-mediated antimicrobial activities, more recent studies have highlighted the involvement of JNK signaling in the Imd pathway response. JNK signaling occurs in a transitory burst and drives the expression of a number of gene products through the AP-1 transcription factor. In this report, we describe a simple method for the quantification of JNK activation by Western blot analysis or directly in tissue culture plates.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Bond
authors.forename: David A
authors.initials: DA
authors.lastname: Primrose
authors.forename: Edan
authors.initials: E
authors.lastname: Foley