Intracellular calcium concentration changes initiated by N-methyl-D-aspartic acid receptors in retinal horizontal cells.
Intracellular calcium concentration changes initiated by N-methyl-D-aspartic acid receptors were studied in carp retinal horizontal cells. Fura-2 fluorescent calcium imaging showed that H1 subtype horizontal cells responded to exogenously applied N-methyl-D-aspartic acid with a transient intracellular free Ca(2+) ([Ca2+]i) increase that decayed to a sustained, but elevated level of [Ca(2+)]i. Contributions of different Ca(2+) flux pathways underlying the time course of this increase in [Ca(2+)]i were explored via experiment as well as via a computational model based on the biophysical properties of H1 cells. Intracellular calcium stores were suggested to play crucial role in the initial transient increase of [Ca(2+)]i.
Wang
Xu-Long
XL
Shanghai Jiao Tong University, Shanghai, China.
Jiang
Xiao-Dong
XD
Liang
Pei-Ji
PJ
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002347:Carps; D003593:Cytoplasm / Q000737:chemistry*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018690:Excitatory Amino Acid Agonists / Q000378:metabolism; D016202:N-Methylaspartate / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D051248:Retinal Horizontal Cells / Q000378:metabolism*
D018690:Excitatory Amino Acid Agonists; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D002118:Calcium