title: Dewetting of polystyrene thin films on poly(ethylene glycol)-modified surfaces as a simple approach for patterning proteins.
abstract: A simple technique for patterning proteins utilizing dewetted polystyrene (PS) droplets is demonstrated. A polystyrene thin film was spin coated on a poly(ethylene glycol) (PEG) silane-modified surface. As the PS film dewets from the surface, upon annealing, to form droplets, the PEG-silane-modified surface is exposed, which retains its capability to resist protein adsorption, and the PS droplets allow the selective adsorption of proteins. In contrast to the undewetted flat PS film, the droplet surface had a greater amount of adsorbed proteins. Atomic force microscopy scans reveal that the roughness of the droplet surface is higher, and a multilayer of proteins results on the droplet surface. Moreover, micro- and nanoscale droplet patterns can easily be achieved by tuning the thickness of PS thin films. Because dewetting approaches for generating ordered dewetting droplets have been successfully generated by others, those approaches could be easily combined with this technique to fabricate ordered protein patterns.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325-3906, USA.
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Cai
authors.forename: Bi-min Zhang
authors.initials: BM
authors.lastname: Newby
mesh_terms: D000327:Adsorption; D000818:Animals; D001709:Biotechnology / Q000379:methods; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D015202:Protein Engineering / Q000379:methods; D012709:Serum Albumin / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: D020099:Coated Materials, Biocompatible; D011137:Polystyrenes; D012709:Serum Albumin; D011092:Polyethylene Glycols; D012825:Silicon