Restricted-access media development for direct analysis of drugs in biofluids using capillary liquid chromatography.
In analytical sciences the design of novel materials and stationary phases for the sample preparation and separation of analytes from biological fluids is needed. In this work we present different strategies for modification of stationary phases to produce tailored solutions for the analytical problem. In this context a novel shielded polymeric reversed-phase monolithic material was prepared in the presence of different numbers of reactive groups and concentrations of the coating polymer. Chromatographic experiments were performed using benzoic acid propyl ester in order to characterize the hydrophobicity and efficiency of the different restricted-access continuous beds prepared. Inverse size-exclusion chromatography was used for investigation of the pore structure properties of the beds. Capillary columns were applied for nanochromatography of biological fluids containing a mixture of nitrazepamum and medazepamum.
Jarmalaviciene
Reda
R
Department of Chemistry, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos 8, 44404, Kaunas, Lithuania.
Kornysova
Olga
O
Bendokas
Vidmantas
V
Westerlund
Douglas
D
Buszewski
Boguslaw
B
Maruska
Audrius
A
D000818:Animals; D001826:Body Fluids / Q000737:chemistry*; D053801:Capillary Electrochromatography / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011108:Polymers; D016062:Porosity
D004364:Pharmaceutical Preparations; D011108:Polymers