Radioprotective effects of chlorogenic acid against mortality induced by gamma-irradiation in mice.
The radioprotective effects of the naturally occurring compound chlorogenic acid have been investigated against mortality induced by gamma-irradiation in mice. Chlorogenic acid was administrated at single doses of 100, 200 and 400 mg/kg 1 or 24 h prior to lethal dose of gamma-irradiation (8.5 Gy). At 30 days after treatment, the percentage of survival in each group was as follows: control, 20%; 100 mg/kg, 20% and 15%; 200 mg/kg, 45% and 15%; 400 mg/kg, 25% and 35% for 1 h and 24 h treatment prior gamma-irradiation, respectively. Survival rate was statistically increased in animals treated with this agent at 200 mg/kg at 1 h prior to irradiation as compared with the irradiation only group. Other doses of chlorogenic acid have not showed any enhanced survival when it was administrated at 1 or 24 h before irradiation. Chlorogenic acid exhibited concentration-dependent activity on 1,1-diphenyl-2-picrylhydrazyl free radical to show strong antioxidant activity. It appeared that chlorogenic acid with antioxidant activity reduced mortality induced by gamma-irradiation.
Hosseinimehr
S J
SJ
Department of Medicinal Chemistry, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran. sjhosseinim@yahoo.com
Zakaryaee
V
V
Ahmadi
A
A
Akhlaghpoor
S
S
D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001713:Biphenyl Compounds; D002726:Chlorogenic Acid / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D051379:Mice; D010851:Picrates / Q000737:chemistry; D011833:Radiation Injuries, Experimental / Q000401:mortality / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*
D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011837:Radiation-Protective Agents; D002726:Chlorogenic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl