Cytochrome c is released from coupled mitochondria of yeast en route to acetic acid-induced programmed cell death and can work as an electron donor and a ROS scavenger.
To gain insight into the processes by which acetic acid-induced programmed cell death (AA-PCD) takes place in yeast, we investigated both cytochrome c release from yeast mitochondria and mitochondrial coupling over the time course of AA-PCD. We show that the majority of cytochrome c release occurs early in AA-PCD from intact coupled mitochondria which undergo only gradual impairment. The released cytochrome c can be reduced both by ascorbate and by superoxide anion and in turn be oxidized via cytochrome c oxidase, thus working both as a ROS scavenger and a respiratory substrate. Late in AA-PCD, the released cytochrome c is degraded.
Giannattasio
Sergio
S
CNR, Istituto di Biomembrane e Bioenergetica, I-70126 Bari, and Trani (BA), Italy.
Atlante
Anna
A
Antonacci
Lucia
L
Guaragnella
Nicoletta
N
Lattanzio
Paolo
P
Passarella
Salvatore
S
Marra
Ersilia
E
D019342:Acetic Acid / Q000378:metabolism* / Q000494:pharmacology; D017209:Apoptosis; D001205:Ascorbic Acid / Q000494:pharmacology; D045304:Cytochromes c / Q000378:metabolism*; D004583:Electrons; D016166:Free Radical Scavengers / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D045304:Cytochromes c; D001205:Ascorbic Acid; D019342:Acetic Acid