title: Non-labeled detection of waterborne pathogen Cryptosporidium parvum using a polydiacetylene-based fluorescence chip.
abstract: A non-labeling fluorescence sensor system was developed using polydiacetylene (PDA) liposomes composed of 10,12-pentacosadiynoic acid (PCDA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) at a 8:2 molar ratio. The PDA liposomes were immobilized onto an amine-coated glass surface using peptide bonding between the carboxyl group of the liposome and the amine group of the glass surface. The optimum ratio of the cross linker (NHS/EDC) to PDA liposome was determined to be 50% for strong immobilization of the liposomes. Residual carboxyl groups of the PDA liposomes were selectively biotinylated, followed by sequential binding of streptavidin and biotin-antibody (bioreceptor). Finally, the performance of the PDA liposome chip was tested for detecting Cryptosporidium parvum, and yielded a detection limit of 1 x 10(3) oocysts/mL. From these results, it is expected that the PDA liposome chip will have high application potential for the detection of waterborne pathogens including C. parvum.
authors.affiliation: Department of Chemical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Cheon Kyo
authors.initials: CK
authors.lastname: Park
authors.forename: Chang Duk
authors.initials: CD
authors.lastname: Kang
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Sim
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D015169:Colony Count, Microbial / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling; D014867:Water / Q000469:parasitology*; D014873:Water Pollutants / Q000032:analysis*
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D014873:Water Pollutants; D014867:Water; D053279:Polyynes; C082361:polydiacetylene