title: Is the Sequenced Bordetella pertussis strain Tohama I representative of the species?
abstract: Subtractive hybridization was carried out to identify differences between the sequenced genome of Bordetella pertussis Tohama I and those of two recently collected isolates. We identified genetic regions specific to recent isolates, old isolates, and isolates of B. parapertussis and B. bronchiseptica species. We conclude that Tohama I strain is not representative of the B. pertussis species.
authors.affiliation: Institut Pasteur, Molecular Prevention and Therapy of Human Diseases Unit, URA-CNRS 3012, National Center of Reference of Whooping Cough and Bordetelloses, Paris, France. vcaro@pasteur.fr
authors.forename: Valérie
authors.initials: V
authors.lastname: Caro
authors.forename: Valérie
authors.initials: V
authors.lastname: Bouchez
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
mesh_terms: D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; C040158:GTAC-specific type II deoxyribonucleases