title: Subtle genomic alterations and genomic instability revealed in diploid cancer cell lines.
abstract: To investigate if genomic instability exists in tumors with cytogenetically normal karyotypes, we analyzed four diploid cancer cell lines A204, CAL51, CH1 and SK-UT-1B. We detected subtle genomic changes in all four cell lines. More of these alterations were found in A204 and CH1 than in the microsatellite unstable lines CAL51 and SK-UT-1B. The number of de novo, non-clonal chromosome rearrangements was also significantly higher in CH1 than CAL51 and SK-UT-1B (p=0.001). This study reveals multiple genomic abnormalities in tumors with near normal karyotypes and suggests that genomic instability may be essential in cancer development.
authors.affiliation: Centre of Medical Oncology, Institute of Cancer, Barts and London School of Medicine and Dentistry, Queen Mary, University of London, Charterhouse Square, London EC1M 6BQ, UK.
authors.forename: Xueying
authors.initials: X
authors.lastname: Mao
authors.forename: Bryan D
authors.initials: BD
authors.lastname: Young
authors.forename: Tracy
authors.initials: T
authors.lastname: Chaplin
authors.forename: Janet
authors.initials: J
authors.lastname: Shipley
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Lu
mesh_terms: D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D004171:Diploidy; D042822:Genomic Instability; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D018895:Microsatellite Repeats; D009369:Neoplasms / Q000235:genetics*