title: Incubation type Si-based planar ion channel biosensor.
abstract: A new planar-type ion channel biosensor with the function of cell culture has been fabricated using silicon on an insulator substrate as the sensor chip material. Coating of the sensor chip with fibronectin was essentially important for cell incubation on the chip. Although the seal resistance was quite low (approximately 7 Mohms) compared with the pipette patch-clamp gigaohm seal, the whole-cell channel current of the transient receptor potential vanilloid type 1 (TRPV1) channel expressing HEK293 cells was successfully observed, with a good signal-to-noise ratio, using capsaicin as a ligand molecule.
authors.affiliation: Institute for Molecular Science, Myodaiji, Okazaki, 444-8585, Japan. urisu@ims.ac.jp
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Urisu
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Asano
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Uno
authors.forename: Ryugo
authors.initials: R
authors.lastname: Tero
authors.forename: Han
authors.initials: H
authors.lastname: Junkyu
authors.forename: Isoda
authors.initials: I
authors.lastname: Hiroko
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Arima
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
authors.forename: Koji
authors.initials: K
authors.lastname: Shibasaki
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002211:Capsaicin / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D007473:Ion Channels / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008024:Ligands; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods; D012825:Silicon / Q000737:chemistry*; D050916:TRPV Cation Channels / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism
chemical_list: D007473:Ion Channels; D008024:Ligands; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D002211:Capsaicin; D012825:Silicon