Synthesis, characterization, and studies on DNA binding of a new Mg(II) complex with N1,N8-bis(1-methyl-4-nitropyrrole-2-carbonyl)triethylenetetramine.
A new Mg(II) complex of MgL(NO3)2 (here L = N(1),N(8)-bis(1-methyl-4-nitropyrrole-2-carbonyl)triethylenetetramine) has been synthesized and characterized. The interactions between the Mg(II) complex and calf thymus DNA has been investigated using UV spectra, fluorescent spectra, viscosity, thermal denaturation, and molecular modeling. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. The experimental results show that the mode of binding of the complex to DNA is non-classical electrostatic action and the complex can cleave pBR322 DNA.
Zhou
Cheng-Yong
CY
Department of Chemistry, Changzhi University, Shanxi Changzhi, PR China. cyzhou@sxu.edu.cn
Wu
Yan-Bo
YB
Yang
Pin
P
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium / Q000737:chemistry; D008958:Models, Molecular; D009691:Nucleic Acid Denaturation; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
C529091:(N(1),N(8)-bis(1-methyl-4-nitropyrrole-2-carbonyl)triethylenetetramine)magnesium(II); D009942:Organometallic Compounds; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium