title: A rapid method for isolation of piperine from the fruits of Piper nigrum Linn.
abstract: A simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of Piper nigrum. The method involves extraction of the fruit powder with glacial acetic acid, from which piperine is partitioned into chloroform and subsequently crystallized. The identity of the compound was confirmed by its melting point, comparison of UV, IR, and mass spectral data with those from a reference standard, and co-chromatography with the reference standard using thin-layer chromatography (TLC). The purity of the compound was ascertained by TLC, by recording UV absorption spectra at the start, middle, and end positions of the spot on the plate, and by differential scanning calorimetry (DSC).
authors.affiliation: Department of Phytochemistry and Pharmacognosy, B. V. Patel Pharmaceutical Education and Research Development Centre, Thaltej-Gandhinagar highway, Thaltej, Ahmedabad, 380 054, Gujarat, India.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Kanaki
authors.forename: Mansi
authors.initials: M
authors.lastname: Dave
authors.forename: Harish
authors.initials: H
authors.lastname: Padh
authors.forename: Mandapati
authors.initials: M
authors.lastname: Rajani
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification*; D052117:Benzodioxoles / Q000302:isolation & purification*; D002152:Calorimetry, Differential Scanning; D002855:Chromatography, Thin Layer; D005638:Fruit; D029222:Piper nigrum / Q000737:chemistry*; D010880:Piperidines / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D053284:Polyunsaturated Alkamides / Q000302:isolation & purification*; D012015:Reference Standards; D013057:Spectrum Analysis; D044366:Transition Temperature
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D010880:Piperidines; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; C008922:piperine