[Amniotic membrane for ocular surface reconstruction after conjunctival squamous cell carcinoma resection].
OBJECTIVE
This study was designed to evaluate the use of human amniotic membrane for ocular surface reconstruction after conjunctival squamous cell carcinoma resection.


METHODS
Amniotic membrane was obtained at the time of cesarean section and was preserved at -80 masculineC in glycerol and cornea culture media at a ratio of 1:1. The inclusion criteria were patients presenting proliferating lesions suggestive of squamous cell carcinoma (flat or elevated white lesions resembling "fish meat") that involve the conjunctiva, limbus and cornea. Eight eyes of 8 patients with conjunctival "squamous cell carcinoma" underwent tumor resection with amniotic membrane transplantation. Three of these cases underwent total corneal epitheliectomy and amniotic membrane transplantation associated with limbal autograft.


RESULTS
Mean follow-up time was 17.8 months (range, 10-35 months). In four patients (71.4%) surgical treatment was successful, with good ocular surface stability. In two patients (28.6%) results were partially successful, with mild cicatricial alterations. One patient was excluded from the study due to aggressive tumor recurrence with intraocular invasion that needed to be removed with exenteration.


CONCLUSIONS
This study suggests that amniotic membrane transplantation is a good alternative for ocular surface reconstruction after conjunctival squamous cell carcinoma resection.
Carvalho-Rêgo
Paulo Roberto de
PR
Setor de Córnea e Doenças Externas, Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil. paulo.r.carvalho@globo.com
Gomes
José Alvaro Pereira
JA
Ballalai
Priscila Luppi
PL
Cunha
Marcelo Carvalho
MC
Sousa
Luciene Barbosa de
LB
Erwenne
Clélia Maria
CM
D000368:Aged; D000369:Aged, 80 and over; D000650:Amnion / Q000637:transplantation*; D002294:Carcinoma, Squamous Cell / Q000601:surgery*; D003230:Conjunctival Neoplasms / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D016896:Treatment Outcome; D055815:Young Adult