title: [The splenic inferior pole of rats and hyperbaric oxygen].
abstract: OBJECTIVE
  To study the functional and morphological features of the lower pole of the spleen in rats submitted, or not, to postoperative hyperbaric oxygen therapy.
  
  
  METHODS
  Seventy-nine Wistar rats, weighing 248.7 +/- 27 g, divided into two groups [group A - simulation (n=40), group B - lower pole (n=39)] underwent surgery and were subdivided into two groups: 11 and 70 postoperative days. Each subgroup was subdivided into animals not treated (nt) (A11nt, n=10; B11nt, n=13; A70nt, n=10; B70nt, n=9) and treated with hyperbaric oxygen (t) (A11t, n=10; B11t, n=9; A70t, n=10; B70t, n=8). Blood was collected for measurement of lipids and immunoglobulins, platelet and Howell-Jolly body count before and after surgery. The spleen and lower pole were removed for histology.
  
  
  RESULTS
  There was an increase of total cholesterol (p=0.002), VLDL-cholesterol and triglycerides (p=0.003) and of LDL-cholesterol (p=0.013) in subgroup B11nt, and no alterations were observed in the other subgroups. IgM decreased in subgroups B11t (p=0.007), B11nt (p=0.0000), B70nt (p=0.0005), B70t (p=0.02), and no change was observed in the simulation group. The number of platelets increased in subgroups B11nt (p=0.002) and B11t (p=0.01) and remained unchanged in the other subgroups. Howell-Jolly bodies were less numerous in subgroup B70nt than in subgroup B11nt (p=0.019). Lower pole viability was better in subgroup B11t than in B11nt and in subgroup B70 than in B11, and did not differ between subgroups B70t and B70nt.
  
  
  CONCLUSIONS
  Function and viability of the remaining lower pole improved during the late postoperative period. Viability and function of the lower pole were better during the early but not during the late postoperative period, in animals submitted to hyperbaric oxygen therapy.
authors.affiliation: Laboratório da Disciplina de Fundamentos da Cirurgia, Escola Superior de Ciências, Santa Casa de Misericórdia de Vitória, ES, Brazil.
authors.forename: Isabel Cristina Andreatta Lemos
authors.initials: IC
authors.lastname: Paulo
authors.forename: Danilo Nagib Salomão
authors.initials: DN
authors.lastname: Paulo
authors.forename: Thiago Antunes
authors.initials: TA
authors.lastname: Ferrari
authors.forename: Thiago Caetano Valadão de
authors.initials: TC
authors.lastname: Azeredo
authors.forename: Alcino Lázaro da
authors.initials: AL
authors.lastname: Silva
mesh_terms: D000818:Animals; D004812:Epidemiologic Methods; D006931:Hyperbaric Oxygenation; D007074:Immunoglobulin G / Q000097:blood; D008026:Ligation / Q000379:methods; D008055:Lipids / Q000097:blood; D008297:Male; D023421:Models, Animal; D009504:Neutrophils / Q000473:pathology; D010976:Platelet Count; D011184:Postoperative Period; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000473:pathology* / Q000502:physiology* / Q000601:surgery; D013156:Splenectomy
chemical_list: D007074:Immunoglobulin G; D008055:Lipids