Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes.
In this work, a new biomaterial resulting from the isolation of octopus rhodopsin (OR) starting from octopus photoreceptor membranes is presented. Mass spectroscopic characterization was employed in order to verify the presence of rhodopsin in the extract. Photoreversibility and photochromic properties were investigated using spectrophotometric measurements and pulsed light. Thin films of OR were realized using the gel-matrix entrapment method in polyvinyl alcohol solution. The results indicate that the photoreversibility and the photostability of the OR in gel-matrices are maintained. Several measurements were performed to test the stability of the resulting biomaterial in time and at room temperature. Preliminary tests demonstrate that the photoreversibility and the photostability are still found after few days from the biomaterial preparation and after the exposure for several hours at room temperature.
Paternolli
Cristina
C
Fondazione Elba, Piazza SS, Apostoli 66, 00100 Rome, Italy.
Neebe
Martin
M
Stura
Enrico
E
Barbieri
Francesca
F
Ghisellini
Paola
P
Hampp
Norbert
N
Nicolini
Claudio
C
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry*; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D049831:Octopodiformes; D010777:Photochemistry; D017956:Photoreceptor Cells, Invertebrate / Q000737:chemistry* / Q000166:cytology; D012160:Retina / Q000737:chemistry / Q000166:cytology; D012243:Rhodopsin / Q000737:chemistry / Q000302:isolation & purification; D013053:Spectrophotometry / Q000379:methods
D001672:Biocompatible Materials; D012243:Rhodopsin