title: Auto-immune neutropenia associated with metformin in a patient with splenic marginal zone lymphoma.
abstract: A 75-year-old man with splenic marginal zone lymphoma had been treated by fludarabine. The 3 first courses were very well tolerated, with no cytopenia. To treat diabetes mellitus, metformin was added at day 6 after the beginning of the fourth course of fludarabine treatment. He had previously been briefly treated with metformin a few months before. Four days later, at day 10, haemogram revealed severe neutropenia (neutrophils: 0.7 g/l). Neutrophil count continued to decrease reaching 0.283 g/l at day 17. A bone marrow aspirate was performed and showed very active granulopoiesis with maturation arrest at the promyelocyte stage. Metformin was discontinued on day 22 (16 days after reintroduction) and neutropenia promptly reversed during next week with 1.9 g/l (day 29). Direct and indirect granulocyte immunofluorescence test were used to detect anti-neutrophil autoantibodies. The antibodies were related to metformin and were detectable by granulocyte agglutination test and the monoclonal antibody-specific immobilization of granulocyte antigens assay. A possible explanation is the fludarabine-metformin combination, since fludarabine could deplete regulator T-lymphocytes and lead to the emergence of metformin-induced anti-neutrophil antibodies.
authors.affiliation: Department of Pharmacy, Purpan Hospital, Toulouse University Hospital, Place du Dr.Baylac, 330 Avenue de Grande Bretagne, 31000 Toulouse, France. franck.laporte@yahoo.fr
authors.forename: Franck
authors.initials: F
authors.lastname: Laporte
authors.forename: Safouane
authors.initials: S
authors.lastname: Hamdi
authors.forename: Françoise
authors.initials: F
authors.lastname: Oksman
authors.forename: Françoise
authors.initials: F
authors.lastname: Huguet
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents / Q000009:adverse effects / Q000627:therapeutic use; D001327:Autoimmune Diseases / Q000139:chemically induced*; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007004:Hypoglycemic Agents / Q000009:adverse effects* / Q000627:therapeutic use; D007958:Leukocyte Count; D018442:Lymphoma, B-Cell, Marginal Zone / Q000150:complications* / Q000188:drug therapy; D008297:Male; D008687:Metformin / Q000009:adverse effects* / Q000627:therapeutic use; D009503:Neutropenia / Q000139:chemically induced*; D013160:Splenic Neoplasms / Q000150:complications* / Q000188:drug therapy; D014740:Vidarabine / Q000009:adverse effects / Q000031:analogs & derivatives / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D007004:Hypoglycemic Agents; D008687:Metformin; D014740:Vidarabine; C024352:fludarabine