[Effect of aurora A on carcinogenesis of human prostate cancer].
OBJECTIVE
To investigate whether Aurora A is involved in prostate cancer carcinogenesis.


METHODS
The expressions of Aurora A mRNA and protein in prostate cancer tissue and cell line were measured using RT-PCR, immunohistochemistry and Western blot analyses. Meanwhile, A DNAzyme targeting at Aurora A mRNA was performed to detect the inhibition effect of Aurora A in regulating the growth of prostate cancer cell line PC3.


RESULTS
Aurora A expression was up-regulated in 91% prostate cancer tissue as well as in prostate cancer cell lines such as PC3, LNCaP and Du145. Furthermore, Aurora A expression suppressed by cell transduction of a DNAzyme targeting at Aurora A mRNA made the PC3 cells both arrest the cell cycle in G2/M phase and increase the cell apoptosis.


CONCLUSIONS
These findings suggest that Aurora A up-regulation may be involved in carcinogenesis of prostate cancer, and that aurora A gene may be a valuable target for treatment of prostate cancer.
Qu
Yi
Y
Experimental Center, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Zhang
Ling
L
Mu
De-Zhi
DZ
Huang
Xiang
X
Wei
Da-peng
DP
Zhao
Feng-yan
FY
Liu
Bai-lin
BL
D017209:Apoptosis; D064127:Aurora Kinases; D002453:Cell Cycle; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015854:Up-Regulation
D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases