title: Severe vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model.
abstract: Allergic asthma is a complex immunologically mediated disease associated with increased oxidative stress and altered antioxidant defenses. It was hypothesized that alpha-tocopherol (alpha-T) decreases oxidative stress and therefore its absence may influence allergic inflammatory process, a pathobiology known to be accompanied by oxidative stress. Therefore, selected parameters of allergic asthma sensitization and inflammation were evaluated following ovalbumin sensitization and re-challenge of alpha-T transfer protein (TTP) knock-out mice (TTP(-/-)) that have greatly reduced lung alpha-T levels (e.g.<5%) compared to their litter mate controls (TTP(+/+)). Results showed that severe alpha-T deficiency result in a blunted lung expression of IL-5 mRNA and IL-5 protein and plasma IgE levels compared with TTP(+/+) mice following immune sensitization and rechallenge, although lung lavage eosinophil levels were comparable in both genomic strains. It is concluded that the initial stimulation of immune responses by the TTP(-/-) mice were generally blunted compared to the TTP(+/+) mice, thus diminishing some aspects of subsequent allergic inflammatory processes.
authors.affiliation: Department of Internal Medicine, Center for Comparative Lung Biology and Disease, School of Medicine, Davis, CA 95616, USA.
authors.forename: Yunsook
authors.initials: Y
authors.lastname: Lim
authors.forename: Vihas T
authors.initials: VT
authors.lastname: Vasu
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Valacchi
authors.forename: Scott
authors.initials: S
authors.lastname: Leonard
authors.forename: Hnin Hnin
authors.initials: HH
authors.lastname: Aung
authors.forename: Bettina C
authors.initials: BC
authors.lastname: Schock
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Kenyon
authors.forename: Chin-Shang
authors.initials: CS
authors.lastname: Li
authors.forename: Maret G
authors.initials: MG
authors.lastname: Traber
authors.forename: Carroll E
authors.initials: CE
authors.lastname: Cross
mesh_terms: D000818:Animals; D001249:Asthma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D016535:Bronchial Hyperreactivity / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics; D004804:Eosinophils / Q000378:metabolism; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation; D015848:Interleukin-5 / Q000378:metabolism; D008168:Lung / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D010047:Ovalbumin / Q000737:chemistry; D018384:Oxidative Stress; D014811:Vitamin E Deficiency / Q000150:complications*
chemical_list: D002352:Carrier Proteins; D015848:Interleukin-5; C017763:alpha-tocopherol transfer protein; D007073:Immunoglobulin E; D010047:Ovalbumin