[Start codon in the serine proteinase gene from Bacillus intermedius].
The translation initiation site in the extracellular serine subtilisin-like proteinase gene from Bacillus intermedius (aprBi) (AN AY754946) secreting at the stationary growth phase was established. The analysis of aprBi open reading frame revealed three putative translation start sites (TTG, GTG, ATG). Using SignalP online freeware program we have determined the functional activity probability of each of them. To identify the translation start point the modified subtilisin-like protease genes carrying nucleotide replacements in supposed start codons were developed using oligonucleotide-directed mutagenesis. We have investigated the expression of these genetic constructions in protease-deficient strain B. subtilis AJ73. According our results it was concluded that the translation in aprBi gene starts from GTG kodon.
Kaiumov
A R
AR
Sabirova
A R
AR
Balaban
N P
NP
Mardanova
A M
AM
Il'inskaia
O N
ON
Kostrov
S V
SV
Sharipova
M R
MR
D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D018387:Codon, Initiator / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D010442:Peptide Chain Initiation, Translational / Q000235:genetics; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D012984:Software
D001426:Bacterial Proteins; D018387:Codon, Initiator; D012697:Serine Endopeptidases