Exposure of sticklebacks (Gasterosteus aculeatus) to cadmium sulfide nanoparticles: biological effects and the importance of experimental design.
Effects of nanoparticles on aquatic organisms have been little studied to date and toxicological data are urgently needed for development of regulatory frameworks for these substances. Here, we report the findings of a study exposing sticklebacks to cadmium sulfide (CdS) as bulk material and quantum dots. Fish were exposed for 21 d in a flow through test system to 5, 50 or 500 microg l(-1) CdS nanoparticles (nCdS) coated in thiol terminated methyl polyethylene glycol (MPEG), bulk CdS or MPEG at 500 microg l(-1) (nominal concentrations). With the exception of the highest nCdS exposure, measured concentrations were approximately one order of magnitude below nominal. A single fish from each group (excluding MPEG) was examined using energy dispersive X-ray analysis (EDX) to localise cadmium, however, cadmium could not be detected in whole body sections. Elevated levels of oxidized glutathione were measured in the gills of fish exposed to 50 and 500 microg l(-1) nCdS. Induction of vitellogenin synthesis was not detected in any of the treatment groups. The number of males engaged in nest-building behaviour following exposure to 500 microg l(-1) nCdS was reduced and livers of 4/6 fish in the same treatment displayed hepatocellular nuclear pleomorphism. The results are discussed emphasising the fundamental importance of experimental design and the need to understand the behaviour of nanoparticles in the aqueous phase.
Sanders
Matthew B
MB
Cefas Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset, UK.
Sebire
Marion
M
Sturve
Joachim
J
Christian
Paul
P
Katsiadaki
Ioanna
I
Lyons
Brett P
BP
Sheahan
Dave
D
Weeks
Jason M
JM
Feist
Stephen W
SW
D000818:Animals; D019187:Cadmium Compounds / Q000633:toxicity*; D005880:Gills / Q000737:chemistry / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D009425:Nesting Behavior / Q000187:drug effects; D010865:Pilot Projects; D012107:Research Design; D023701:Smegmamorpha / Q000502:physiology*; D013440:Sulfides / Q000633:toxicity*
D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D005978:Glutathione