title: The anti-inflammatory modulatory role of Solidago chilensis Meyen in the murine model of the air pouch.
abstract: The aim of this study was to investigate the anti-inflammatory efficacy of an aqueous extract (AE), and its butanolic (BuOH) and aqueous residual (AR) fractions, derived from the rhizome of Solidago chilensis in inflammation caused by carrageenan in mice. Solidago chilensis Meyen rhizome was extracted using hot water at 90 degrees C under infusion. The extract was filtered and lyophilized. Part of the aqueous extract was fractionated with n-BuOH, resulting in butanolic (BuOH) and aqueous residual (AR) fractions. Adult Swiss mice were used in the in-vivo experiments. We evaluated the effect of rhizome aqueous extract of Solidago chilensis and these two derived fractions on the inflammation induced by carrageenan in the mouse model of the air pouch. The aqueous extract and its derived fractions significantly inhibited leucocytes, neutrophils, exudation, myeloperoxidase and adenosine deaminase activity, as well as nitric oxide, interleukin-1 beta (IL-1beta), neutrophil chemokine (KC) and tumour necrosis factor-alpha (TNF-alpha) levels (P < 0.05). Indometacin and dexamethasone inhibited all the studied inflammatory parameters (P < 0.01) with the exceptions that indometacin did not inhibit TNF-alpha levels and dexamethasone did not inhibit KC levels (P > 0.05). These results indicate that Solidago chilensis has a significant anti-inflammatory action on acute inflammatory responses and that its inhibitory activity may be due not only to the inhibition of proinflammatory mediators, but also to the inhibition of leucocyte infiltration.
authors.affiliation: Department of Clinical Analysis, Centre of Health Sciences, Federal University of Santa Catarina, Campus Universitário-Trindade, 88040-970, Florianópolis, SC, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Liz
authors.forename: Silvana Virginia Gagliotti
authors.initials: SV
authors.lastname: Vigil
authors.forename: Stella
authors.initials: S
authors.lastname: Goulart
authors.forename: Maria Izabel Goularte
authors.initials: MI
authors.lastname: Moritz
authors.forename: Eloir Paulo
authors.initials: EP
authors.lastname: Schenkel
authors.forename: Tânia Silvia
authors.initials: TS
authors.lastname: Fröde
mesh_terms: D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan; D002465:Cell Movement / Q000187:drug effects; D018925:Chemokines / Q000378:metabolism; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control; D053583:Interleukin-1beta / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D027343:Rhizome / Q000737:chemistry; D036624:Solidago / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D018925:Chemokines; D053583:Interleukin-1beta; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D002351:Carrageenan; D009195:Peroxidase; D000243:Adenosine Deaminase