title: Development of magnetically separable immobilized lipase by using cellulose derivatives and their application in enantioselective esterification of ibuprofen.
abstract: Highly active, stable, and magnetically separable immobilized enzymes were developed using carboxymethyl cellulose (CMC) and diethylaminoethyl cellulose DEAE-C; hereafter designated "DEAE" as supporting materials. Iron oxide nanoparticles penetrated the micropores of the supporting materials, rendering them magnetically separable. Lipase (LP) was immobilized on the surface of the supporting materials by using cross-linked enzyme aggregation (CLEA) by glutaraldehyde. The activity of enzyme aggregates coated on DEAE was approximately 2 times higher than that of enzyme aggregates coated on CMC. This is explained by the fact that enzyme aggregates with amine residues are more efficient than those with carboxyl residues. After a 96-h enantioselective ibuprofen esterification reaction, 6% ibuprofen propyl ester was produced from the racemic mixture of ibuprofen by using DEAE-LP, and 2.8% using CMC-LP.
authors.affiliation: Department of Chemical Engineering, Chosun University, Kwangju 501-759, Korea.
authors.forename: Gowoun
authors.initials: G
authors.lastname: Lee
authors.forename: Hongil
authors.initials: H
authors.lastname: Joo
authors.forename: Jungbae
authors.initials: J
authors.lastname: Kim
authors.forename: Jung-Heon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000201:enzymology; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D003636:DEAE-Cellulose / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000648:ultrastructure; D004951:Esterification; D007052:Ibuprofen / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000648:ultrastructure; D008280:Magnetics; D011355:Prodrugs / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D011355:Prodrugs; D002482:Cellulose; D003636:DEAE-Cellulose; D008049:Lipase; D002266:Carboxymethylcellulose Sodium; D007052:Ibuprofen