title: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells.
abstract: Generally, the 3'-end of the duplex microRNA (miR) precursor (pre-miR) is known to be stable in vivo and serve as a mature form of miR. However, both the 3'-end (miR9) and 5'-end (miR9*) of a brain-specific miR9 have been shown to function biologically in brain development. In this study, real-time PCR analysis and in vitro/in vivo bioluminescent imaging demonstrated that the upstream region of a primary miR9-1 (pri-miR9-1) can be used to monitor the highly expressed pattern of endogenous pri-miR9-1 during neurogenesis, and that the Luciferase reporter gene can image the unequal expression patterns of miR9 and miR9* seen during the neuronal differentiation of P19 cells. This demonstrates that our bioimaging system can be used to study the participation of miRs in the regulation of neuronal differentiation.
authors.affiliation: Programs in Neuroscience, Seoul National University, Korea.
authors.forename: Mee Hyang
authors.initials: MH
authors.lastname: Ko
authors.forename: Soonhag
authors.initials: S
authors.lastname: Kim
authors.forename: Do Won
authors.initials: DW
authors.lastname: Hwang
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Ko
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Dong Soo
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000196:embryology; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000502:physiology*
chemical_list: D035683:MicroRNAs