title: Anaerobic degradation of p-xylene in sediment-free sulfate-reducing enrichment culture.
abstract: Anaerobic degradation of p-xylene was studied with sulfate-reducing enrichment culture. The enrichment culture was established with sediment-free sulfate-reducing consortium on crude oil. The crude oil-degrading consortium prepared with marine sediment revealed that toluene, and xylenes among the fraction of alkylbenzene in the crude oil were consumed during the incubation. The PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene for the p-xylene degrading sulfate-reducing enrichment culture showed the presence of the single dominant DGGE band pXy-K-13 coupled with p-xylene consumption and sulfide production. Sequence analysis of the DGGE band revealed a close relationship between DGGE band pXy-K-13 and the previously described marine sulfate-reducing strain oXyS1 (similarity value, 99%), which grow anaerobically with o-xylene. These results suggest that microorganism corresponding to pXy-K-13 is an important sulfate-reducing bacterium to degrade p-xylene in the enrichment culture.
authors.affiliation: College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Shinya
authors.initials: S
authors.lastname: Sato
authors.forename: Manabu
authors.initials: M
authors.lastname: Fukui
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D020564:Deltaproteobacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010578:Petroleum / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013431:Sulfates / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D014992:Xylenes; C031286:4-xylene