title: Altered expression of 12S/MT-RNR1, MT-CO2/COX2, and MT-ATP6 mitochondrial genes in prostate cancer.
abstract: BACKGROUND
  Prostate cancer is one of the commonest cancers worldwide and is responsible for nearly 6% of all male cancer deaths. Despite this relevance, the mechanisms involved in the development and progression of this malignancy remain unknown. The involvement of polypeptides of the mitochondrial respiratory chain, the Krebs cycle and the glutathione antioxidant system in this type of cancer has been previously described, although no publication has focused on the expression of mitochondrial genes in the prostate of PCa patients.
  
  
  METHODS
  We have determined by reverse transcription-quantitative PCR (RT-qPCR) the relative amount of the transcripts of eight mitochondrial genes (MT-ND2, MT-ND4, MT-ND6, MT-CYB, 12S/MT-RNR1, 16S/MT-RNR2, MT-CO2/COX2, MT-ATP6), and four nuclear genes (COX11, GSR, CS, ACO2), all of them key players in the normal metabolism of mitochondria. Additionally we analyzed the expression of Cyclophilin A (PPIA).
  
  
  RESULTS
  We observed differential expression of mitochondrial 12S/MT-RNR1, MT-CO2/COX2, and MT-ATP6 transcripts in tumor samples when compared to their paired normal samples.
  
  
  CONCLUSIONS
  The amount of mitochondrial 12S/MT-RNR1, MT-CO2/COX2, and MT-ATP6 transcripts is significantly decreased in tumor samples when compared to their paired normal sample, suggesting that mitochondrial gene expression is altered in PCa.
authors.affiliation: Centre de Genètica Mèdica i Molecular-IDIBELL, Hospital Duran i Reynals, L'Hospitalet de Llobregat, Barcelona, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Abril
authors.forename: Miguel López
authors.initials: ML
authors.lastname: de Heredia
authors.forename: Laura
authors.initials: L
authors.lastname: González
authors.forename: Ramon
authors.initials: R
authors.lastname: Clèries
authors.forename: Marga
authors.initials: M
authors.lastname: Nadal
authors.forename: Enric
authors.initials: E
authors.lastname: Condom
authors.forename: Fernando
authors.initials: F
authors.lastname: Aguiló
authors.forename: Montserrat
authors.initials: M
authors.lastname: Gómez-Zaera
authors.forename: Virginia
authors.initials: V
authors.lastname: Nunes
mesh_terms: D000154:Aconitate Hydratase / Q000235:genetics; D000368:Aged; D000079922:Copper Transport Proteins; D004268:DNA-Binding Proteins / Q000235:genetics*; D045222:Electron Transport Chain Complex Proteins; D003576:Electron Transport Complex IV / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D050259:Genes, Mitochondrial / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D009245:NADH Dehydrogenase / Q000235:genetics; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D011471:Prostatic Neoplasms / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C484188:COX11 protein, human; D000079922:Copper Transport Proteins; D004268:DNA-Binding Proteins; D045222:Electron Transport Chain Complex Proteins; C501005:MT-ATP6 protein, human; D024101:Mitochondrial Proteins; C539882:NR4A2 protein, human; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D014157:Transcription Factors; C507225:MT-ND6 protein, human; D009245:NADH Dehydrogenase; C521326:NADH dehydrogenase subunit 2, human; C117108:cytochrome C oxidase subunit II; D003576:Electron Transport Complex IV; D025261:Mitochondrial Proton-Translocating ATPases; C476079:ACO2 protein, human; D000154:Aconitate Hydratase