[Biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts].
OBJECTIVE
To investigate biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts (HPDLF) for verifying the hypothesis of delivering medicine to the periodontium and whole body through the root canal.


METHODS
HPDLF and MC3T3-E1 cells were incubated in antibiotics solutions. The intracellular antibiotics contents were measured by high performance liquid chromatography (HPLC) and the cell total protein was measured by bradford protein assay.


RESULTS
The intracellular contents increased with incubation time. The extracellular medicine concentration had effect on the intracellular contents.


CONCLUSIONS
Tetracycline hydrochloride can be transported into HPDLF with incubation and this transport is time-dependent and concentration-dependent.
Liu
Yu
Y
Dental Institute, General Hospital of PLA, Beijing 100853, China.
Liu
Hong-chen
HC
Wu
Xia
X
E
Ling-ling
LL
Leng
Bin
B
D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D001692:Biological Transport; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D010513:Periodontal Ligament / Q000378:metabolism*; D013752:Tetracycline / Q000493:pharmacokinetics*
D000900:Anti-Bacterial Agents; D013752:Tetracycline