[Adaptation of free and total plasma carnitine determination on the Dimension HM, X-Pand model (Dade Behring)].
L-carnitine is a quaternary ammonium compound that facilitates long-chain fatty acids crossing through the mitochondrial membrane allowing their beta-oxidation. Human pathologies relatives to carnitine are mainly deficiencies, with myopathy and lipids metabolism disorders. The aim of this study was to validate the free and total carnitine plasmatic level measurement using the Dimension HM, X-Pand model analyzer (Dade Behring). Free carnitine was directly determined on plasma samples, deproteinized by using ultrafiltration, whereas total carnitine was measured after alkaline hydrolysis. Within-day and between-day imprecision were < 4,00 and 9,00 % respectively. The method is linear through 250,00 micromol/L and the limit of quantification is 3,00 micromol/L. Our method was correlated to tandem mass spectrometry (r = 0,956 for free and total carnitine). Recovery of free and total carnitine from spiked plasmas was > 99 %. On board stability of cartridge (5 days) and of calibration (at least 30 days) are in agreement with a method easily adaptable in routine laboratories.
Kerspern
H
H
Service de biochimie et biologie moléculaire, CHU de Brest. helene.kerspern@chu-brest.fr
Carré
J-L
JL
D001331:Automation; D002138:Calibration; D002331:Carnitine / Q000031:analogs & derivatives / Q000097:blood*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D008297:Male; D013058:Mass Spectrometry; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D013997:Time Factors; D014462:Ultrafiltration
D007202:Indicators and Reagents; C116917:acylcarnitine; D002331:Carnitine