Pueraria mirifica, phytoestrogen-induced change in synaptophysin expression via estrogen receptor in rat hippocampal neuron.
OBJECTIVE
To examine Pueraria mirifica (Leguminosae) containing-phytoestrogen effect on synaptic density and involvement of estrogen receptor.


METHODS
The level of synaptophysin, a presynaptic vesicle protein, was measured using Western blot analysis and immunocytochemistry in hippocampal primary cell cultures at 6 days in vitro.


RESULTS
P. mirifica and 17beta-estradiol (0.1 microM) treatment for 4 days, but not for 2 days, significantly increased synaptophysin immunoreactivity and level of synaptophysin. P. mirifica up to 60 microg/ml resulted in a dose related increase in the level of synaptophysin immunoreactivity. The classical estrogen receptor antagonist, ICI 182 780, significantly blocked P. mirifica-induced increase in synaptophysin.


CONCLUSIONS
P. mirifica-containing phytoestrogen affects synaptic density by inducing synaptophysin expression via estrogen receptor.
Chindewa
Rattiya
R
Center for Neuroscience, Faculty of Science, Mahidol University, Bangkok, Thailand.
Lapanantasin
Saitida
S
Sanvarinda
Yupin
Y
Chongthammakun
Sukumal
S
D000818:Animals; D004958:Estradiol; D005260:Female; D006624:Hippocampus / Q000187:drug effects*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D023421:Models, Animal; D009474:Neurons / Q000187:drug effects*; D048789:Phytoestrogens; D028321:Plant Preparations / Q000494:pharmacology*; D011247:Pregnancy; D029908:Pueraria; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000187:drug effects*; D016708:Synaptophysin / Q000096:biosynthesis / Q000187:drug effects*; D013997:Time Factors
D048789:Phytoestrogens; D028321:Plant Preparations; D011960:Receptors, Estrogen; D016708:Synaptophysin; D004958:Estradiol