title: Blue native electrophoresis study on lipases.
abstract: We have developed a modified blue native polyacrylamide gel electrophoresis (PAGE) protocol that can overcome aggregation of lipases seen in native PAGE. We have shown that two lipases, Pseudomonas aeruginosa lipase and Candida rugosa lipase, which aggregate in the native gel, can be resolved using our protocol. Activity staining was done to test for the functionality of the two lipases.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India.
authors.forename: M
authors.initials: M
authors.lastname: Saminathan
authors.forename: K Thirumalai
authors.initials: KT
authors.lastname: Muthukumaresan
authors.forename: Srinivas
authors.initials: S
authors.lastname: Rengarajan
authors.forename: Nandhini
authors.initials: N
authors.lastname: Muthukrishnan
authors.forename: Pennathur
authors.initials: P
authors.lastname: Gautam
mesh_terms: D002175:Candida / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008049:Lipase / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D013194:Staining and Labeling
chemical_list: D008049:Lipase