title: Anti-inflammatory action of alpha-melanocyte stimulating hormone (alpha-MSH) in anti-CD3/CD28-mediated spleen and CD4(+)CD25(-) T cells and a partial participation of IL-10.
abstract: alpha-Melanocyte stimulating hormone (alpha-MSH) has been shown to inhibit the production and the effects of pro-inflammatory cytokine by inflammatory cells in innate immunity. We have determined whether alpha-MSH inhibits anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells proliferation and its mechanism of action. The proliferation of anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells markedly were suppressed by 50-100 nM and 5-100 nM alpha-MSH, respectively. alpha-MSH (100 nM) increased the production of the anti-inflammatory cytokine IL-10 and decreased the production of the pro-inflammatory cytokine IL-2 and IFN-gamma from CD4(+)CD25(-) T cells. Moreover, anti-IL-10 blocking Ab decreased the inhibitory effects of anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells proliferation by alpha-MSH, indicating a partial participation of IL-10 in its mechanism of inhibitory action. These results suggest that alpha-MSH may be useful for treatment of autoimmune diseases and transplantation involving innate and adaptive immunity.
authors.affiliation: Department of Surgery and Asan Institute for Life Sciences, Ulsan University College of Medicine & Asan Medical Center, Songpa-Gu, Seoul, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Chang
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Jung
authors.forename: Dong Gyun
authors.initials: DG
authors.lastname: Lim
authors.forename: Youn Hee
authors.initials: YH
authors.lastname: Park
authors.forename: Yu Mee
authors.initials: YM
authors.lastname: Wee
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Monica Young
authors.initials: MY
authors.lastname: Choi
authors.forename: Sun Kyung
authors.initials: SK
authors.lastname: Koo
authors.forename: Kang Duk
authors.initials: KD
authors.lastname: Choi
authors.forename: Duck Jong
authors.initials: DJ
authors.lastname: Han
authors.forename: Song Cheol
authors.initials: SC
authors.lastname: Kim
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000187:drug effects* / Q000276:immunology*; D000521:alpha-MSH / Q000494:pharmacology*
chemical_list: D015703:Antigens, CD; D018106:CD28 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D016753:Interleukin-10; D000521:alpha-MSH; D017493:Leukocyte Common Antigens