[Development and clinical application of male human papillomavirus genotyping by membrane DNA chip].
OBJECTIVE
To develop a new method for the detection of male human papillomavirus (HPV) genotypes and to investigate its clinical application value.


METHODS
With computer assistance and based on the classical common primers MY09/11, modified PGMY09/11 with 23 HPV subtypes for PCR and Genbank data on HPV, we designed probes for the simultaneous detection of 18 high-risk subtypes (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83 and MM4) and 5 low-risk subtypes (HPV-6, 11, 42, 43 and 44) and fixed them to the special membrane to make a DNA chip. A total of 112 male urethral samples were collected with swabs and studied for the clinical value. Meanwhile the single subtypes of HPV positive were sequenced and the standard samples detected for their sensitivity.


RESULTS
Of the total number, 25 samples were found to be HPV positive, 13 single HPV infection and 12 multiple infection. Nine HPV gene subtypes were noted in the samples: 6, 11, 16, 18, 33, 35, 43, 56 and 73, with sensitivity up to 10 copies of HPV DNA.


CONCLUSIONS
Human papillomavirus genotyping by the membrane DNA chip is applicable to the diagnosis of male HPV infection as well as to the related epidemic and etiological investigation.
Jin
Yu-Ji
YJ
Department of Clinical Laboratory, Shenzhen Children's Hospital, Shenzhen, Guangdong 518026, China.
Yue
Li-Jie
LJ
Tao
Lin
L
Deng
Fang-Mei
FM
Li
De-Fa
DF
He
Wen-Shan
WS
Liu
Jie
J
Xiang
Jian
J
Cai
Hong
H
Tu
Qian-Qian
QQ
Hong
Min
M
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015344:DNA Probes, HPV / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*
D015344:DNA Probes, HPV; D004279:DNA, Viral