title: In vivo imaging of presynaptic terminals and postsynaptic sites in the mouse submandibular ganglion.
abstract: Much of what is currently known about the behavior of synapses in vivo has been learned at the mammalian neuromuscular junction, because it is large and accessible and also its postsynaptic acetylcholine receptors (AChRs) are readily labeled with a specific, high-affinity probe, alpha-bungarotoxin (BTX). Neuron-neuron synapses have thus far been much less accessible. We therefore developed techniques for imaging interneuronal synapses in an accessible ganglion in the peripheral nervous system. In the submandibular ganglion, individual preganglionic axons establish large numbers of axo-somatic synapses with postganglionic neurons. To visualize these sites of synaptic contact, presynaptic axons were imaged by using transgenic mice that express fluorescent protein in preganglionic neurons. The postsynaptic sites were visualized by labeling the acetylcholine receptor (AChR) alpha7 subunit with fluorescently tagged BTX. We developed in vivo methods to acquire three-dimensional image stacks of the axons and postsynaptic sites and then follow them over time. The submandibular ganglion is an ideal site to study the formation, elimination, and maintenance of synaptic connections between neurons in vivo.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors.forename: Corey M
authors.initials: CM
authors.lastname: McCann
authors.forename: Jeff W
authors.initials: JW
authors.lastname: Lichtman
mesh_terms: D000818:Animals; D002038:Bungarotoxins / Q000493:pharmacokinetics; D005726:Ganglia, Parasympathetic / Q000166:cytology*; D021621:Imaging, Three-Dimensional; D007395:Interneurons / Q000166:cytology*; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009469:Neuromuscular Junction / Q000033:anatomy & histology* / Q000378:metabolism; D017729:Presynaptic Terminals / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects / Q000502:physiology; D018800:Thy-1 Antigens / Q000235:genetics; D013997:Time Factors
chemical_list: D002038:Bungarotoxins; D008164:Luminescent Proteins; D018800:Thy-1 Antigens