The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome.
The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.
Gilany
Kambiz
K
Department of Biomedical Sciences, Protein Chemistery, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
Van Elzen
Roos
R
Mous
Kim
K
Coen
Edmond
E
Van Dongen
Walter
W
Vandamme
Stefaan
S
Gevaert
Kris
K
Timmerman
Evy
E
Vandekerckhove
Joël
J
Dewilde
Sylvia
S
Van Ostade
Xaveer
X
Moens
Luc
L
D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009363:Neoplasm Proteins; D020543:Proteome