[The expression rule of proliferating cell nuclear antigen in rat thyroid of hyperplasia induced by propylthiouracil].
OBJECTIVE
To study the expression rule of proliferating cell nuclear antigen (PCNA) in thyroid when the hyperplasia of male rat thyroid is induced by propylthiouracil (PTU).


METHODS
PTU was administered to rats by gavage at a dose of 5.0 mg/kg B. W for 0,3,6,9 and 12 days respectively. All animals were sacrificed after the last dosage, and the expression of PCNA in thyroid was detected by immunohistochemistry and RT-PCR.


RESULTS
Compared with the control group, the PCNA level in the thyroid of rats given PTU for 3 days significantly increased (P < 0.05),and reached the highest expression when it was 6 days, but then when it was 9 days and 12 days, the PCNA expression showed a descending tendency.


CONCLUSIONS
PCNA has a expression rule in hyperplastic thyroid: expression increase first but then decrease. This rule confirms the histological change of thyroid in earlier stage. The experimental time of assessment test to thyroid hormone disruptors will be shortened to six days.
Zhang
Su-Cai
SC
Department of Pathology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Wang
Yu
Y
Yang
Kai-xuan
KX
Cheng
Wei-bo
WB
Shang
Wei-wei
WW
Wang
Wen-dong
WD
D000818:Animals; D013956:Antithyroid Agents / Q000009:adverse effects*; D006965:Hyperplasia / Q000378:metabolism; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011441:Propylthiouracil / Q000009:adverse effects*; D051381:Rats; D013961:Thyroid Gland / Q000473:pathology*
D013956:Antithyroid Agents; D018809:Proliferating Cell Nuclear Antigen; D011441:Propylthiouracil