Early blood-brain barrier permeability in cerebella of PLSJL mice immunized with myelin basic protein.
The blood-brain barrier (BBB) is dramatically but transiently compromised in the cerebella of myelin basic protein immunized mice at least 1 week prior to the development of the paralytic phase of experimental allergic encephalomyelitis (EAE). Treatment of mice with the peroxynitrite-dependent radical scavenger uric acid (UA) during the first week after immunization blocks the early increase in cerebellar BBB permeability and the subsequent development of clinical signs of EAE. These results indicate that the early loss of BBB integrity in the cerebellum is likely to be a necessary step in the development of paralytic EAE.
Spitsin
Sergei
S
Thomas Jefferson University, 1020 Locust St., JAH room 470C, Philadelphia, PA 19107, United States. sspitsin@mail.jci.tju.edu
Portocarrero
Carla
C
Phares
Timothy W
TW
Kean
Rhonda B
RB
Brimer
Christine M
CM
Koprowski
Hilary
H
Hooper
D Craig
DC
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000276:immunology*; D002199:Capillary Permeability / Q000187:drug effects / Q000276:immunology; D002531:Cerebellum / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D019793:Fluorescein; D005786:Gene Expression Regulation / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000235:genetics / Q000378:metabolism; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018709:Statistics, Nonparametric; D013997:Time Factors
D015703:Antigens, CD; D016177:Macrophage-1 Antigen; D004676:Myelin Basic Protein; D018799:Intercellular Adhesion Molecule-1; D052247:Nitric Oxide Synthase Type II; D019793:Fluorescein