Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
Gasser
Brigitte
B
University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria. diethard.mattanovich@boku.ac.at.
Saloheimo
Markku
M
Rinas
Ursula
U
Dragosits
Martin
M
Rodríguez-Carmona
Escarlata
E
Baumann
Kristin
K
Giuliani
Maria
M
Parrilli
Ermenegilda
E
Branduardi
Paola
P
Lang
Christine
C
Porro
Danilo
D
Ferrer
Pau
P
Tutino
Maria Luisa
ML
Mattanovich
Diethard
D
Villaverde
Antonio
A