Involvement of septal Cdk5 in the emergence of excessive anxiety induced by stress.
The aim of the present study was to evaluate whether the activation of Cdk5, a protein that has been suggested to participate in higher cognitive functions, is required for the onset of a sensitized anxiety-related behavior induced by stress. The exposure to restraint enhanced both Cdk5 expression in certain subareas of the septohippocampal system, principally in the lateral septum (LS) and septal Cdk5 kinase activity in rats. Behaviorally, restrained wild type mice showed a behavior indicative of enhanced anxiety in the elevated plus maze (EPM). In contrast, unstressed mice and stressed knockout mice, which lacked the p35 protein, the natural activator of Cdk5, displayed similar anxiety-like behavior in the EPM. Finally, the intra-LS infusion of olomoucine - a Cdk5 inhibitor - blocked the enhanced anxiety in the EPM induced by prior stress in rats. All these data provide evidence that septal Cdk5 is required in the emergence of a sensitized emotional process induced by stress.
Bignante
Elena Anahi
EA
Departamento de Farmacología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Argentina.
Rodriguez Manzanares
Pablo Andrés
PA
Mlewski
Estela Cecilia
EC
Bertotto
María Eugenia
ME
Bussolino
Daniela Fernanda
DF
Paglini
Gabriela
G
Molina
Victor Alejandro
VA
D000704:Analysis of Variance; D000818:Animals; D001007:Anxiety / Q000209:etiology / Q000473:pathology* / Q000517:prevention & control; D001522:Behavior, Animal; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D007701:Kinetin / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020665:Septum of Brain / Q000201:enzymology*; D013315:Stress, Psychological / Q000150:complications*
D004791:Enzyme Inhibitors; C090046:olomoucine; D051360:Cyclin-Dependent Kinase 5; C495907:Cdk5 protein, rat; D007701:Kinetin