Screening isolates from antibody phage-display libraries.
Antibody phage display, coupled with automated screening, facilitates and potentiates the mining of complex combinatorial libraries and the identification of potent drug leads. In managing phage screening data, the behavior of individual phage isolates in binding assays must be linked to their antibody identities as deduced from DNA sequencing. Reviewed here are recently reported approaches for high-throughput screening of clones isolated from phage antibody libraries after selection on a defined antigen. Specific information management challenges, and possible solutions, are described for organizing screening data to enable rapid lead discovery using these antibody libraries.
Buckler
David R
DR
Dyax Corp., 300 Technology Square, Cambridge, MA 02139, USA. dbuckler@dyax.com
Park
Albert
A
Viswanathan
Malini
M
Hoet
Rene M
RM
Ladner
Robert C
RC
D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000302:isolation & purification; D019151:Peptide Library; D040081:Protein Array Analysis; D011506:Proteins / Q000276:immunology
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D011506:Proteins