Resistance, serotype and genetic diversity of Streptococcus pneumoniae-resistant strains isolated in the West Pomerania region of Poland in the years 2001-2005.
The aim of this study was to analyse the resistance patterns, serotypes and genetic diversity of Streptococcus pneumoniae-resistant strains isolated in the West Pomerania region of Poland. They were clinical isolates obtained during a 5-year study (2001-2005) mainly from ambulatory patients with upper respiratory tract infections. The strains showed resistance to 8 out of 9 tested antibiotics (except vancomycin) and 53.8% of the strains were multidrug-resistant (MDR). The increase over time in the number of MDR strains and in resistance degrees was not statistically significant. Resistance to cotrimoxazole was the most frequent (86.7%). Penicillin nonsusceptibility was shown in 38% of the strains and resistance to macrolides in 36.7% of the strains, mainly of MLS(B) phenotype (94.1%). A significant resistance increase was only observed for beta-lactam antibiotic. The population of S. pneumoniae-resistant strains in our region presented 31 resistance patterns, 13 serotypes and a high genetic diversity-70 pulse field gel electrophoresis (PFGE) profiles have been described: 44 of them were unique and 26 clusters consisted of 2 to 30 strains similar by more than 87%. Cluster I, grouping 30 strains of similar resistant patterns (TSH: 70%, SH, TH, T, H, S) and mainly serotype 19F, isolated over the 5 years of the study, could represent a new national clone. The polysaccharide 23-valent vaccine covers 83.5%, while the conjugated 7-, 9- and 11-valent vaccines cover 79.1-79.7% of the resistant strains collected in our region. A statistically significant decrease of vaccine coverage in time has been noted.
Nowosiad
M
M
Department of Microbiology and Immunology, Pomeranian Medical University, Powstańców Wielkopolskich Street 72, Szczecin, Poland. monikan@wp.pl
Giedrys-Kalemba
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011008:Pneumococcal Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D022242:Pneumococcal Vaccines; D011044:Poland / Q000453:epidemiology; D012703:Serotyping; D018709:Statistics, Nonparametric; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D055815:Young Adult
D000900:Anti-Bacterial Agents; D022242:Pneumococcal Vaccines