Correlation between plasma Flt3-ligand concentration and hematopoiesis during G-CSF-induced CD34+ cell mobilization.
This study aimed to correlate blood Flt3-ligand (FL) concentration with CD34(+) cell number in blood and bone marrow (BM) during granulocyte colony-stimulating factor (G-CSF) mobilization. Nonhuman primates were injected with 10 microg/kg of G-CSF (Lenograstim) daily over a period of 5 days. Daily blood sampling and repeated BM sampling showed that FL concentration before mobilization was negatively correlated to the absolute number of BM CD34(+) cells, but also to the number of G-CSF-mobilized CD34(+) cells on days 3-5 of treatment. This showed that FL concentration in the blood reflected BM status before mobilization, and suggested that this parameter could be used as a predictive indicator of G-CSF-induced CD34(+) cell mobilization.
Bertho
Jean-Marc
JM
Institut de Radioprotection et de Sûreté Nucléaire, DRPH/SRBE, Fontenay aux Roses, France. jean-marc.bertho@irsn.fr
Prat
Marie
M
Stefani
Johanna
J
Demarquay
Christelle
C
Simon
Erwan
E
Dudoignon
Nicolas
N
Thierry
Dominique
D
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D000078224:Lenograstim; D008252:Macaca fascicularis; D008297:Male; D008565:Membrane Proteins / Q000097:blood*; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors
D000276:Adjuvants, Immunologic; D018952:Antigens, CD34; D008565:Membrane Proteins; D011994:Recombinant Proteins; C084881:flt3 ligand protein; D016179:Granulocyte Colony-Stimulating Factor; D000078224:Lenograstim