Proliferation and interleukin 5 production by CD8hi CD57+ T cells.
CD8hi CD57+ T cells have previously been described as effector memory T cells with minimal expansion capacity and high susceptibility to activation-induced cell death. In contrast, we demonstrate here that CD8hi CD57+ T cells are capable of rapid expansion using multiple techniques including [(3)H]thymidine uptake, flow cytometric bead-based enumeration and standard haemocytometer counting. Previous reports can be explained by marked inhibition of activation-induced expansion and increased 7-amino-actinomycin D uptake by CD8hi CD57+ T cells following treatment with CFSE, a dye previously used to measure their proliferation, combined with specific media requirements for the growth of this cell subset. The ability of CD8hi CD57+ T cells to further differentiate is highlighted by a distinct cytokine profile late after activation that includes the unexpected release of high levels of interleukin 5. These data indicate that CD8hi CD57+ T cells should not be considered as "end-stage" effector T cells incapable of proliferation, but represent a highly differentiated subset capable of rapid division and exhibiting novel functions separate from their previously described cytotoxic and IFN-gamma responses.
Chong
Lee K
LK
Department of Medical Biochemistry & Immunology, School of Medicine, Cardiff University, Cardiff, UK.
Aicheler
Rebecca J
RJ
Llewellyn-Lacey
Sian
S
Tomasec
Peter
P
Brennan
Paul
P
Wang
Eddie C Y
EC
D000328:Adult; D018959:CD57 Antigens / Q000276:immunology*; D016827:CD8 Antigens / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003609:Dactinomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D005452:Fluoresceins / Q000494:pharmacology; D006801:Humans; D015848:Interleukin-5 / Q000096:biosynthesis*; D008875:Middle Aged; D013388:Succinimides / Q000494:pharmacology
C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D018959:CD57 Antigens; D016827:CD8 Antigens; D005452:Fluoresceins; D015848:Interleukin-5; D013388:Succinimides; D003609:Dactinomycin; C025942:7-aminoactinomycin D