Effects of prednisolone on specifically expressed genes in pediatric acute B-lymphoblastic leukemia.
Although glucocorticoid is essential in the treatment of pediatric acute lymphoblastic leukemia (ALL), their precise mechanisms of action remain unclear. We used DNA microarray to evaluate prednisolone-regulated genes in pre-B-ALL cells from 2 pediatric patients. We found up-regulation of 26 genes in ALL cells from both patients, compared with peripheral normal B lymphocytes before maintenance chemotherapy. Treatment with prednisolone for 48 hours induced down-regulation of 5 genes (terminal deoxynucleotidyl transferase, heparin-binding epidermal growth factorlike growth factor, pre-B-lymphocyte genes 1 and 3, and immunoglobulin lambda-like polypeptide) among 26 specifically expressed genes in pre-B-ALL cells from both patients.
Akiyama
Masaharu
M
Department of Pediatrics, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo, Japan. makiyama@jikei.ac.jp
Yamada
Osamu
O
Agawa
Miyuki
M
Yuza
Yuki
Y
Yanagisawa
Takaaki
T
Eto
Yoshikatsu
Y
Yamada
Hisashi
H
D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007223:Infant; D016393:Lymphoma, B-Cell / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000235:genetics*; D011241:Prednisone / Q000627:therapeutic use*; D013902:Radiography, Thoracic; D014057:Tomography, X-Ray Computed
D011241:Prednisone