Oxygen tension and medium type actions on blastocyst development and interferon-tau secretion in cattle.
Most current in vitro production systems terminate at the blastocyst stage in cattle. The goal of the present research was to identify culture conditions that support individual blastocyst survival and interferon-tau (IFNT) production in cattle. In the first study, two media (medium 199 [M199] and potassium simplex optimized medium [KSOM]) and two oxygen tensions (5 and 20%) were compared for their ability to sustain blastocyst survival and IFNT production from days 8 to 11 post-insemination. Survival and total cell numbers were greater (P<0.05) for blastocysts cultured in M199 in a 5% oxygen environment compared with other medium and oxygen treatment combinations. Serum supplementation was required for blastocyst survival and IFNT production. IFNT concentrations in conditioned medium were similar for blastocysts cultured in M199 or KSOM, but blastocysts incubated in 5% oxygen produced less (P<0.001) IFNT than their 20% oxygen counterparts. Oxidative stress was not responsible for the increase in IFNT concentrations. Supplementation with fibroblast growth factor 2 did not affect cell numbers but increased (P<0.02) IFNT concentrations for blastocysts cultured in 5% oxygen but not those cultured in 20% oxygen. In conclusion, culturing blastocysts of cattle in a 5% oxygen environment with M199 containing serum sustains embryo viability and permits constitutive and inducible IFNT production. Incubation in 20% oxygen increases IFNT production. The mechanism responsible for this event and its physiological relevance to conceptus development in utero remain unknown.
Rodina
Teresa M
TM
Department of Animal Sciences, University of Florida, Gainesville, 32618, USA.
Cooke
Flavia N T
FN
Hansen
Peter J
PJ
Ealy
Alan D
AD
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001755:Blastocyst / Q000166:cytology*; D002417:Cattle / Q000502:physiology*; D002452:Cell Count / Q000662:veterinary; D002470:Cell Survival / Q000502:physiology; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D007370:Interferon Type I / Q000096:biosynthesis / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000008:administration & dosage*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis / Q000378:metabolism*
D003470:Culture Media; D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau; D010100:Oxygen