title: Novel human adenovirus causing nosocomial epidemic keratoconjunctivitis.
abstract: In 2000, we encountered cases of nosocomial infections with epidemic keratoconjunctivitis (EKC) at a university hospital in Kobe, in the western part of Japan. Two human adenovirus (HAdV) strains, Kobe-H and Kobe-S, were isolated from patients with nosocomial EKC infection. They were untypeable by existing neutralizing antisera; however, the isolate was neutralized with homologous antisera. We then encountered several cases of EKC due to nosocomial infections in eye clinics in different parts of Japan. A total of 80 HAdVs were isolated from patients with EKC at eight different hospitals. The partial hexon gene sequences of the isolates were determined and compared to those of the prototype strains of 51 serotypes. All isolates had identical partial hexon nucleotide sequences. Phylogenetic analysis classified these isolates into species of HAdV-D. The isolates showed 93.9 to 96.7% nucleotide identity with HAdV-D prototype strains, while all 32 HAdV-D prototype strains ranged from 93.2 to 99.2% identity. The sequences of the loop 2 and fiber knob regions from the representative strain, Kobe-H, were dissimilar in all prototype strains of 51 serotypes. We believe that this virus is a novel serotype of HAdV that causes EKC.
authors.affiliation: Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Shimura 3-30-1, Itabashi-ku, Tokyo 174-8555, Japan. Ishiko.Hiroaki@mp.medience.co.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishiko
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimada
authors.forename: Tsunetada
authors.initials: T
authors.lastname: Konno
authors.forename: Akio
authors.initials: A
authors.lastname: Hayashi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohguchi
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Tagawa
authors.forename: Koki
authors.initials: K
authors.lastname: Aoki
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Ohno
authors.forename: Shudo
authors.initials: S
authors.lastname: Yamazaki
mesh_terms: D000258:Adenovirus Infections, Human / Q000453:epidemiology / Q000821:virology; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D036022:Capsid Proteins / Q000235:genetics; D003236:Conjunctivitis, Viral / Q000453:epidemiology / Q000821:virology; D003428:Cross Infection / Q000453:epidemiology / Q000821:virology; D004196:Disease Outbreaks; D006801:Humans; D007564:Japan; D007637:Keratoconjunctivitis / Q000453:epidemiology / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus