Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov.
A novel mesophilic, hydrogenotrophic methanogen, strain SANAET, was isolated from an anaerobic, propionate-degrading enrichment culture, which was originally established from rice paddy soil. The cells were non-motile, Gram-negative and rod-shaped (1.8-2.4 microm long by 0.3-0.6 microm wide). Growth of strain SANAET was observed at 25-40 degrees C, with an optimum temperature range for growth of 35-37 degrees C. The pH range for growth was 6.5-7.8, with an optimum at pH 7.0. The salinity range for growth was 0-1 g NaCl l(-1) (0-17 mM). The isolate was able to utilize H2/CO2 and formate for growth and methane production. The G+C content of the genomic DNA was 56.6 mol%. Based on comparative 16S rRNA gene sequence analysis, strain SANAET was affiliated with a clone lineage of the Archaea, Rice Cluster I (RC-I), placing it between the orders Methanosarcinales and Methanomicrobiales within the class 'Methanomicrobia'. 16S rRNA gene sequence similarities between strain SANAET and members of Methanosarcinales were in the range 80.0-82.8 %, and those between the strain and members of Methanomicrobiales ranged from 77.5 to 82.4 %. In addition to 16S rRNA gene analysis, sequence analysis of the mcrA gene (encoding the alpha subunit of methyl-coenzyme M reductase, a key enzyme in the methane production pathway) also showed that strain SANAET was affiliated with the RC-I lineage. Here, we propose the name Methanocella paludicola gen. nov., sp. nov. for the isolate, the first of the RC-I lineage. The type strain is SANAET (=JCM 13418T=NBRC 101707T=DSM 17711T). In addition, we also propose the status of order for the RC-I lineage, for which we propose the name Methanocellales ord. nov.
Sakai
Sanae
S
Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Imachi
Hiroyuki
H
Hanada
Satoshi
S
Ohashi
Akiyoshi
A
Harada
Hideki
H
Kamagata
Yoichi
Y
D001482:Base Composition; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D019605:Euryarchaeota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019766:Genes, Archaeal; D008697:Methane / Q000096:biosynthesis; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology; D010088:Oxidoreductases / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D009626:Terminology as Topic
D019641:DNA, Archaeal; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C021572:methyl coenzyme M reductase; D008697:Methane