title: Rehmannia inhibits adipocyte differentiation and adipogenesis.
abstract: Rehmannia glutinosa, a Traditional Chinese Medicine (TCM), has been used to increase physical strength. Here, we report that Rehmannia glutinosa extract (RE) inhibits adipocyte differentiation and adipogenesis. RE impairs differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. At the molecular level, treatment with RE inhibits expression of the key adipocyte differentiation regulator C/EBPbeta, as well as C/EBPalpha and the terminal marker protein 422/aP2, during differentiation of preadipocytes into adipocytes. Additionally, RE inhibits the mitotic clonal expansion (MCE) process of adipocyte differentiation, and RE prevents localization of C/EBPbeta to the centromeres. RE also prevents high fat diet (HFD) induced weight gain and adiposity in rats. Taken together, our results indicate that Rehmannia glutinosa extract inhibits preadipocyte differentiation and adipogenesis in cultured cells and in rodent models of obesity.
authors.affiliation: The Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.forename: Nai-xian
authors.initials: NX
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Mo
authors.forename: Rui
authors.initials: R
authors.lastname: Wan
authors.forename: Chun-gu
authors.initials: CG
authors.lastname: Ma
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Yin-liang
authors.initials: YL
authors.lastname: Gu
authors.forename: Xin-ying
authors.initials: XY
authors.lastname: Yang
authors.forename: Qi-qun
authors.initials: QQ
authors.lastname: Tang
authors.forename: Hou-yan
authors.initials: HY
authors.lastname: Song
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002503:Centromere / Q000737:chemistry / Q000378:metabolism; D004032:Diet; D004195:Disease Models, Animal; D005223:Fats / Q000008:administration & dosage / Q000009:adverse effects; D050556:Fatty Acid-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009765:Obesity / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; C493411:Fabp4 protein, mouse; D005223:Fats; D050556:Fatty Acid-Binding Proteins; D010936:Plant Extracts