title: Anti-allergic effect of a combination of Citrus unshiu unripe fruits extract and prednisolone on picryl chloride-induced contact dermatitis in mice.
abstract: Effect of 50% ethanolic extract of unripe fruits of Citrus unshiu (CU-ext) on type IV allergic reaction was examined by inhibitory activity of ear swelling of picryl chloride-induced contact dermatitis (PC-CD) in mice. Oral administration of CU-ext and subcutaneous administration of prednisolone showed inhibition of ear swelling during both induction and effector phases of PC-CD. The inhibitory activities of combinations of CU-ext (p.o.) and prednisolone (s.c.) during induction phase of PC-CD were more potent than those of CU-ext alone and prednisolone alone. Successive oral administration of hesperidin, a major flavanone glycoside of CU-ext, inhibited ear swelling during induction phase of PC-CD. The inhibitory activities of combinations of hesperidin (p.o.) and prednisolone (s.c.) were more potent than those of hesperidin alone and prednisolone alone. These results indicated that the combinations of prednisolone and CU-ext or hesperidin exerted a synergistic effect.
authors.affiliation: School of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka, 577-0818, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Fujita
authors.forename: Takehumi
authors.initials: T
authors.lastname: Shiura
authors.forename: Megumi
authors.initials: M
authors.lastname: Masuda
authors.forename: Masashi
authors.initials: M
authors.lastname: Tokunaga
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawase
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iwaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Gato
authors.forename: Masahiko
authors.initials: M
authors.lastname: Fumuro
authors.forename: Katsuaki
authors.initials: K
authors.lastname: Sasaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Utsunomiya
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002957:Citrus; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000209:etiology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D004487:Edema / Q000209:etiology / Q000517:prevention & control; D004890:Erythema / Q000209:etiology / Q000517:prevention & control; D005260:Female; D005638:Fruit; D005938:Glucocorticoids / Q000008:administration & dosage / Q000627:therapeutic use*; D006569:Hesperidin / Q000494:pharmacology / Q000627:therapeutic use; D007279:Injections, Subcutaneous; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010853:Picryl Chloride; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011239:Prednisolone / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D018926:Anti-Allergic Agents; D005938:Glucocorticoids; D010936:Plant Extracts; D011239:Prednisolone; D006569:Hesperidin; D010853:Picryl Chloride