17beta-Estradiol enhances expression of inflammatory cytokines and inducible nitric oxide synthase in mouse contact hypersensitivity.
The effects of 17beta-estradiol (E2) on the expression of cytokines, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 in mouse contact hypersensitivity (CHS) were examined. Three week old female mice were ovariectomized, administered 3.2 microg of E2 subcutaneously, the mice sensitized by application of 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (OXA) on their backs, and CHS induced by applying OXA to the auricles. E2 significantly increased mRNA expression of interferon (IFN)-gamma and interleukin (IL)-10 in the auricle at 6 and 24 h after allergy elicitation in the ear, respectively, when compared to untreated controls. Although there was no effect of E2 on the expression of IL-4 and COX-2 at any time, the expression of iNOS mRNA was increased by E2 treatment at 48 h after elicitation. E2 also enhanced the expression of tumor necrosis factor (TNF)-alpha and IL-1beta. Histological evaluation revealed that E2 promoted edema of the auricle dermis. The hyperplasia of the epidermis was suppressed by E2 and the cell infiltration observed after elicitation was not altered by E2. These results suggest that E2 enhances the expression of IFN-gamma, TNF-alpha, and IL-1beta to augment the edema of auricle dermis in mouse CHS.
Sakazaki
Fumitoshi
F
Department of Public Health and Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka 573-0101, Japan.
Ueno
Hitoshi
H
Nakamuro
Katsuhiko
K
D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D017449:Dermatitis, Allergic Contact / Q000378:metabolism* / Q000473:pathology; D054644:Ear Auricle / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007249:Inflammation / Q000378:metabolism*; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D010052:Ovariectomy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D004958:Estradiol; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2