title: 2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis.
abstract: Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Glucuronyltransferase-I (GlcAT-I) is the key enzyme that completes the synthesis of this linkage region, which is a prerequisite for the conversion of core proteins to functional proteoglycans bearing GAGs. The Xyl and Gal residues in the linkage region can be modified by phosphorylation and sulfation, respectively, although the biological significance of these modifications remains to be clarified. Here we present evidence that these modifications can significantly influence the catalytic activity of GlcAT-I. Enzyme assays showed that the synthetic substrates, Gal-Gal-Xyl(2-O-phosphate)-O-Ser and Gal-Gal(6-O-sulfate)-Xyl(2-O-phosphate)-O-Ser, served as better substrates than the unmodified compound, whereas Gal(6-O-sulfate)-Gal-Xyl(2-O-phosphate)-O-Ser exhibited no acceptor activity. The crystal structure of the catalytic domain of GlcAT-I with UDP and Gal-Gal(6-O-sulfate)-Xyl(2-O-phosphate)-O-Ser bound revealed that the Xyl(2-O-phosphate)-O-Ser is disordered and the 6-O-sulfate forms interactions with Gln(318) from the second GlcAT-I monomer in the dimeric enzyme. The results indicate the possible involvement of these modifications in the processing and maturation of the growing linkage region oligosaccharide required for the assembly of GAG chains.
authors.affiliation: Department of Biochemistry, Kobe Pharmaceutical University, Higashinada-ku, Kobe 658-8558, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Tone
authors.forename: Lars C
authors.initials: LC
authors.lastname: Pedersen
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Tomomi
authors.initials: T
authors.lastname: Izumikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kitagawa
authors.forename: Junko
authors.initials: J
authors.lastname: Nishihara
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Tamura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Negishi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sugahara
mesh_terms: D000818:Animals; D002417:Cattle; D019281:Dimerization; D005690:Galactose / Q000737:chemistry*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D008968:Molecular Conformation; D009005:Monosaccharides / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D010766:Phosphorylation; D011509:Proteoglycans / Q000737:chemistry; D013379:Substrate Specificity; D014994:Xylose / Q000737:chemistry*
chemical_list: D006025:Glycosaminoglycans; D009005:Monosaccharides; D009844:Oligosaccharides; D011509:Proteoglycans; D014994:Xylose; C111364:galactosylgalactoylxylosylprotein 3-beta-glucuronosyltransferase; D014453:Glucuronosyltransferase; D005690:Galactose