[Analysis of overexpression of vascular endothelial growth factor-C in patients with angioimmunoblastic T-cell lymphoma].
OBJECTIVE
To explore the vascular endothelial growth factor-C (VEGF-C) expression and its clinical significance in malignant lymphoma.


METHODS
Lymphoma cells were isolated by laser microdissection. VEGF-C expression in lymphoma tissue and microdissected lymphoma cells was measured by realtime quantitative PCR. Meanwhile, vessel ultrastructure was identified by transmission electron microscopy.


RESULTS
Comparing with that in 8 patients with reactive lymphocyte hyperplasia, VEGF-C was overexpressed in angioimmunoblastic T-cell lymphoma, both in lymphoma tissue (n = 18, P = 0.0020) and in microdissected lymphoma cells (n = 10, P < 0.0001). Increased VEGF-C level was associated with bone marrow infiltration (P = 0.0039), skin involvement (P = 0.0046) and high-risk international prognostic index (P = 0.0302). In VEGF-C overexpressed cases, ultrastructural study showed dystrophic vessels, with swelling endothelial cells and absence of pericytes.


CONCLUSIONS
The value of VEGF-C expression might be a biomarker of disease progression in angioimmunoblastic T-cell lymphoma.
Zhao
Wei-li
WL
Department of Hematology, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
Liu
Yan-Yan
YY
Plassa
François
F
Jin
Xiao-Long
XL
Wang
Li
L
Janin
Anne
A
Shen
Zhi-Xiang
ZX
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007119:Immunoblastic Lymphadenopathy / Q000378:metabolism* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
D042582:Vascular Endothelial Growth Factor C