Protein 600 is a microtubule/endoplasmic reticulum-associated protein in CNS neurons.
There is an increasing body of literature pointing to cytoskeletal proteins as spatial organizers and interactors of organelles. In this study, we identified protein 600 (p600) as a novel microtubule-associated protein (MAP) developmentally regulated in neurons. p600 exhibits the unique feature to interact with the endoplasmic reticulum (ER). Silencing of p600 by RNA interference (RNAi) destabilizes neuronal processes in young primary neurons undergoing neurite extension and containing scarce staining of the ER marker Bip. Furthermore, in utero electroporation of p600 RNAi alters neuronal migration, a process that depends on synergistic actions of microtubule dynamics and ER functions. p600-depleted migrating neurons display thin, crooked, and "zigzag" leading process with very few ER membranes. Thus, p600 constitutes the only known MAP to associate with the ER in neurons, and this interaction may impact on multiple cellular processes ranging from neuronal development to neuronal maturation and plasticity.
Shim
Su Yeon
SY
Hotchkiss Brain Institute, Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
Wang
Jian
J
Asada
Naoyuki
N
Neumayer
Gernot
G
Tran
Hong Chi
HC
Ishiguro
Kei-ichiro
K
Sanada
Kamon
K
Nakatani
Yoshihiro
Y
Nguyen
Minh Dang
MD
D000818:Animals; D000831:Animals, Newborn; D002148:Calmodulin-Binding Proteins; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016253:Microscopy, Immunoelectron / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D016900:Neurofilament Proteins / Q000172:deficiency; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure*; D034622:RNA Interference / Q000502:physiology; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
D002148:Calmodulin-Binding Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D014404:Tubulin; C462941:beta3 tubulin, mouse; C044882:neurofilament protein L; D049452:Green Fluorescent Proteins; D044767:Ubiquitin-Protein Ligases; C528904:Ubr4 protein, mouse