title: Bcl-2, CD34 and CD10 expression in basaloid follicular hamartoma, vellus hair hamartoma and neurofollicular hamartoma demonstrate full follicular differentiation.
abstract: Generalized basaloid follicular hamartoma syndrome (GBFHS) is a rare, recently-described, autosomal-dominantly inherited disorder that presents with disseminated milia, palmoplantar pitting, hypotrichosis and basaloid follicular hamartomas (BFH). BFH is a benign adnexal tumor that resembles basal cell carcinoma (BCC). In this study, we report two cases of GBFHS and stain BFH, a vellus hair hamartoma (VHH) and a neurofollicular hamartoma (NH) with CD34, bcl-2 and CD10 to characterize and compare the staining patterns of these follicular tumors. Standard immunohistochemistry labeling with CD34, bcl-2 and CD10 was performed on paraffin-embedded, formalin-fixed tissue sections of five BFH (four for CD10), one VHH and one NH. CD34 stromal staining was observed in all specimens. Bcl-2 stained the outermost cell layers of the basaloid nests in all specimens. CD10 stained the peritumoral stroma of all specimens. The BFH, NFH and the VHH showed CD10 staining of matrical cells. CD34 and CD10 stain peritumoral stroma of BFH, VHH and NH. Bcl-2 stains the outermost cell layer of these tumors. CD10 was also observed to stain matrical cells. These results show the similarities in differentiation between these benign follicular neoplasms and trichoepithelioma.
authors.affiliation: Department of Dermatology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
authors.forename: Frances I
authors.initials: FI
authors.lastname: Ramos-Ceballos
authors.forename: Shayesteh
authors.initials: S
authors.lastname: Pashaei
authors.forename: Jay M
authors.initials: JM
authors.lastname: Kincannon
authors.forename: Michael B
authors.initials: MB
authors.lastname: Morgan
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Smoller
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004814:Epidermal Cyst / Q000378:metabolism / Q000473:pathology; D005260:Female; D006201:Hair Diseases / Q000378:metabolism* / Q000473:pathology; D018859:Hair Follicle / Q000378:metabolism / Q000473:pathology; D006223:Hamartoma Syndrome, Multiple / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D015260:Neprilysin / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D019253:Proto-Oncogene Proteins c-bcl-2; D015260:Neprilysin