A simple and convenient approach for evaluation of the parameters of ligand-receptor interaction. Receptor blocking index and its application.
A new approach for determination of the parameters for ligand-receptor interaction, which is based on so-called dilution coordinates, was developed earlier. Equations that allow evaluation of not only the affinity of ligand-receptor interaction but also of the amount of free (or occupied by corresponding ligand) receptors were suggested. The most important advantage of this approach as compared with well-known methods is the ability to determine the binding parameters for ligand-receptor interaction even for the cases in which ligand and receptor are already present in a mixture and separation of counterparts from each other is technically difficult or even impossible. Due to this reason, the proposed approach can be especially useful for studying interactions between highly-labile biological receptors and corresponding ligands as found in vivo. In the present paper I continue to consider how to determine the binding parameters for a given ligand-receptor interaction if the value of receptor blocking index is determined experimentally.
Bobrovnik
S A
SA
Department of Molecular Immunology, Palladin Institute of Biochemistry, Kiev 01601, Ukraine. s-bobrov@bk.ru
D000818:Animals; D000936:Antigen-Antibody Complex; D002645:Chickens; D007201:Indicator Dilution Techniques; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D008024:Ligands; D011956:Receptors, Cell Surface