title: X-ray diffraction measurement of the monolayer spontaneous curvature of dioleoylphosphatidylglycerol.
abstract: Phosphatidylglycerol (PG) is an anionic lipid commonly found in large proportions in the cell membranes of bacteria and plants and, to a lesser extent, in animal cells. PG plays an important role in the regulation and determination of the elastic properties of the membrane. Using small angle X-ray scattering experiments, we obtain that the monolayer spontaneous curvature of dioleoylphosphatidylglycerol (DOPG) is -1/150+/-0.021 nm(-1) when measured in 150 mM NaCl. When the experiments are carried out in 150 mM NaCl and 20mM MgCl(2), the value obtained for the monolayer spontaneous curvature is -1/8.7+/-0.037 nm(-1). These values are of importance in modelling the effects of curvature elastic stress in membrane lipid homeostasis in the bacterium Acholeplasma laidlawii [Alley, S.H., Barahona, M., Ces, O., Templer, R.H., in press. Biophysical regulation of lipid biosynthesis in the plasma membrane. Biophys. J.] and indicate that divalent cations can play a significant role in altering curvature elastic stress.
authors.affiliation: Department of Bioengineering, Imperial College London, United Kingdom.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Alley
authors.forename: Oscar
authors.initials: O
authors.lastname: Ces
authors.forename: Mauricio
authors.initials: M
authors.lastname: Barahona
authors.forename: Richard H
authors.initials: RH
authors.lastname: Templer
mesh_terms: D004548:Elasticity; D008051:Lipid Bilayers / Q000737:chemistry*; D015636:Magnesium Chloride / Q000737:chemistry; D008560:Membrane Fluidity; D010715:Phosphatidylglycerols / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013314:Stress, Mechanical; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; D012996:Solutions; D015636:Magnesium Chloride; D012965:Sodium Chloride; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol