title: Low-molecular-weight hyaluronan permeates through human intestinal Caco-2 cell monolayers via the paracellular pathway.
abstract: The intestinal permeability of low-molecular-weight hyaluronan (LMW-HA) was investigated by using cultured monolayers of Caco-2 cells. The amount of LMW-HA that permeated the Caco-2 monolayers was measured by a carbazole assay. The permeability of LMW-HA increased inversely with the molecular size and was dose-dependent. The transport was observed to be energy-independent, and was correlated with the tight junction (TJ) permeability. These results suggest that LMW-HA permeated the Caco-2 cell monolayers via the paracellular pathway.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Hisada
authors.forename: Hideo
authors.initials: H
authors.lastname: Satsu
authors.forename: Akira
authors.initials: A
authors.lastname: Mori
authors.forename: Mamoru
authors.initials: M
authors.lastname: Totsuka
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kamei
authors.forename: Takashi
authors.initials: T
authors.lastname: Nozawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D010539:Permeability
chemical_list: D009844:Oligosaccharides; D006820:Hyaluronic Acid