Semisynthesis of dimeric proteins by expressed protein ligation.
A one-pot synthesis of homodimeric proteins is described. The synthetic strategy is based on a double expressed protein ligation reaction between thioester peptides and a new bis-cysteinyl linker. The protocol was also applied to the synthesis of heterodimers.
Ziaco
Barbara
B
Università di Napoli Federico II, Dipartimento delle Scienze Biologiche, Facoltà di Scienze Biotecnologiche, via Mezzocannone 16, 80134 Napoli, Italy.
Pensato
Soccorsa
S
D'Andrea
Luca D
LD
Benedetti
Ettore
E
Romanelli
Alessandra
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003432:Cross-Linking Reagents / Q000737:chemistry; D019281:Dimerization; D008024:Ligands; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013997:Time Factors
D003432:Cross-Linking Reagents; D008024:Ligands; D010455:Peptides; D011506:Proteins; D013438:Sulfhydryl Compounds