Phyllanthus maderaspatensis, a dietary supplement for the amelioration of adriamycin-induced toxicity and oxidative stress in mice.
The effect of ethanol extract of Phyllanthus maderaspatensis (PME), a popular south Indian dietary supplement, was studied for its chemoprotective property on adriamycin (ADR)-induced toxicity and oxidative stress in mice. Adriamycin toxicity was evaluated biochemically by measuring the serum concentration of lactate dehydrogenase (LDH) and creatinine phosphokinase (CPK). Genotoxicity was evaluated by measuring the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in bone marrow cells. Oxidative stress in the heart tissue was estimated by measuring the glutathione (GSH) levels in the homogenate. The treatment of mice with different doses of PME (400 mg/kg and 600 mg/kg body weight, p.o.,) for 7 days before the administration of a single i.p. dose of ADR (15 mg/kg) exhibited significant protection in a dose-dependent manner. The results clearly indicate that PME has a protective effect against ADR-induced toxicity, as revealed by the decrease in the concentrations of LDH, CPK, and the frequency of MNPCEs. The increased levels of GSH are indicative of the antioxidant property of PME.
Bommu
Praveen
P
TIFAC CORE in Herbal Drugs, JSS College of Pharmacy, Ootacamund, 643 001, The Nilgiris, Tamilnadu, India.
Nanjan
Chandrasekar Moola Joghee
CM
Joghee
Nanjan Moola
NM
Nataraj
Satishkumar Muthureddy
SM
Bhojraj
Suresh
S
D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001854:Bone Marrow Cells / Q000187:drug effects; D018890:Chemoprevention; D017024:Chemotherapy, Adjuvant; D003402:Creatine Kinase / Q000097:blood; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000187:drug effects*; D029803:Phyllanthus; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents; D004317:Doxorubicin; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D005978:Glutathione