title: Effects of Smads and BMPs induced by Ga-Al-As laser irradiation on calcification ability of human dental pulp cells.
abstract: We investigated the effects of Ga-Al-As laser irradiation on the mineralization ability of human dental pulp (HDP) cells and on Smads and bone morphogenetic protein (BMP) production as one mechanism for the transmission of laser photochemical energy to cells. HDP cells in vitro were irradiated once with a Ga-Al-As laser at 1.0 W for 500 s, and calcified nodule formation was assessed by Alizarin red S staining. The laser irradiation was greater in the laser-irradiated group than in the non-irradiated group. Both calcium production and alkaline phosphatase (ALP) activity were higher after laser irradiation. Expression of mRNAs for Smad1, Smad7, BMPs, ALP, and osteocalcin was greater after laser irradiation, whereas expression of Smad6 mRNA was inhibited. Production of BMP-2 and BMP-4 in conditioned medium was also higher after laser irradiation. These results suggest that Smads and BMPs play important roles in ALP activity and calcification upon laser irradiation of HDP cells.
authors.affiliation: Department of Clinical Oral Physiology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan. matsui.satoshi@nihon-u.ac.jp
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsui
authors.forename: Hitomi
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000528:radiation effects; D000880:Anthraquinones; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins / Q000032:analysis / Q000502:physiology / Q000528:radiation effects*; D002113:Calcification, Physiologic / Q000502:physiology / Q000528:radiation effects*; D002118:Calcium / Q000032:analysis / Q000528:radiation effects; D002478:Cells, Cultured; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D003782:Dental Pulp / Q000166:cytology / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor; D015675:Osteocalcin / Q000032:analysis / Q000528:radiation effects; D012333:RNA, Messenger / Q000032:analysis; D051785:Smad Proteins / Q000502:physiology / Q000528:radiation effects*; D051898:Smad1 Protein / Q000032:analysis / Q000528:radiation effects; D051905:Smad6 Protein / Q000032:analysis / Q000528:radiation effects; D051906:Smad7 Protein / Q000032:analysis / Q000528:radiation effects; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000528:radiation effects
chemical_list: D000880:Anthraquinones; C525718:BMP2 protein, human; C525809:BMP4 protein, human; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D019485:Bone Morphogenetic Proteins; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D012333:RNA, Messenger; C497399:SMAD1 protein, human; C497802:SMAD6 protein, human; C497806:SMAD7 protein, human; D051785:Smad Proteins; D051898:Smad1 Protein; D051905:Smad6 Protein; D051906:Smad7 Protein; D016212:Transforming Growth Factor beta; D015675:Osteocalcin; C004468:Alizarin Red S; D000469:Alkaline Phosphatase; D002118:Calcium