title: Improved production of ginsenosides in suspension cultures of ginseng by medium replenishment strategy.
abstract: The objective of this study was to improve the accumulation of ginsenosides by the adventitious root cultures of ginseng, which are important secondary metabolites with pharmaceutical applications. The adventitious roots were cultured in bioreactors for 50 d using 1.5-strength Murashige and Skoog (MS) medium supplemented with 10 mg/l indole acetic acid and 75 g/l sucrose. Kinetic studies of the nutrient composition of the spent medium revealed the gradual depletion of various inorganic nutrients and sugars. Cultures were supplied with fresh nutrient medium (medium exchange or replenishment with 0.75- and 1.0-strength MS medium) after 10 and 20 d of culture initiation to fulfill the nutritional requirements of adventitious roots. Medium replenishment strategy (with 1.0-strength MS medium after 20 d of culture) significantly improved the growth of adventitious roots and the biosynthesis of ginsenosides by the adventitious roots. This work is useful for the large-scale cultivation of adventitious roots for the production of ginsenosides.
authors.affiliation: Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, 12-Gaeshin-Dong, Cheongju 361-763, Chugnbuk, South Korea.
authors.forename: Cheol-Seung
authors.initials: CS
authors.lastname: Jeong
authors.forename: Hosakatte Niranjana
authors.initials: HN
authors.lastname: Murthy
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Hahn
authors.forename: Kee-Yoeup
authors.initials: KY
authors.lastname: Paek
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques; D036145:Ginsenosides / Q000096:biosynthesis*; D007210:Indoleacetic Acids / Q000494:pharmacology; D005894:Panax / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology
chemical_list: D036145:Ginsenosides; D007210:Indoleacetic Acids; D013395:Sucrose; C030737:indoleacetic acid