title: Adenoviral-mediated up-regulation of Otos, a novel specific cochlear gene, decreases cisplatin-induced apoptosis of cultured spiral ligament fibrocytes via MAPK/mitochondrial pathway.
abstract: Previous reports have implicated Otos, a novel specific gene expressed by spiral ligament fibrocytes (SLFs) with unclear functions, as a protective gene for cochlea. However, whether Otos gene could protect SLFs against cisplatin (DDP)-induced apoptosis remains largely unknown. In the present study, we utilized Adenoviral-mediated gene transfection to up-regulate Otos expression in cultured SLFs and further assessed the cell viability and apoptosis as well as possible MAPK and mitochondrial pathways. As expected, the data showed that Otos up-regulation significantly decreased apoptosis of SLFs induced by DDP possibly through activation of ERK and partial inhibition of JNK and mitochondrial pathway but not p-38 pathway, suggesting Otos as a potential protective gene for cochlea and raising the possibility of Otos up-regulation as a promising approach to DDP-induced deafness therapy.
authors.affiliation: Department of Otolaryngology, Southwest Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Xian-Lu
authors.initials: XL
authors.lastname: Zhuo
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-Lei
authors.initials: WL
authors.lastname: Zhuo
authors.forename: Yun-Song
authors.initials: YS
authors.lastname: Zhang
authors.forename: Yun-Jun
authors.initials: YJ
authors.lastname: Wei
authors.forename: Xue-Yuan
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis; D002478:Cells, Cultured; D002945:Cisplatin / Q000633:toxicity*; D003051:Cochlea / Q000166:cytology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D051381:Rats; D014162:Transfection; D015854:Up-Regulation
chemical_list: D000970:Antineoplastic Agents; C503047:Otos protein, rat; D011506:Proteins; D020928:Mitogen-Activated Protein Kinases; D002945:Cisplatin