[The expression of human macrophage metalloelastase mRNA in gastric cancer cell lines and tissues and its clinical significance].
OBJECTIVE
To make measurement of the expression of HME mRNA in both gastric cancer cell lines and tissues and evaluate its role in development of gastric cancer.


METHODS
The HME mRNA expression in 3 gastric cancer cell lines and tissues was detected by real-time fluorescent quantitative PCR.


RESULTS
All three gastric cancer cell lines expressed HME mRNA, and the HME mRNA expression level in gastric cancer tissues was higher than that in normal tissues (P <0.05). The rate of lymph node metastasis of HME mRNA positive cases was lower than that of HME mRNA negative ones (P < 0.05). Expression of drug resistance gene GST of HME mRNA positive cases was lower than that of HME mRNA negative ones (P <0.05). The two-year survival rate of HME mRNA positive cases was higher than that of HME mRNA negative ones (P < 0.05). There was no correlation between the expression of HME gene and the tumor location, size, depth of invasion, degree of malignancy, expression of drug resistance gene top II and PG.


CONCLUSIONS
The expression of HME gene in gastric cancers may be related with lower possibility of metastasis and predict a better prognosis.
Zhang
Hong
H
Department of Gastroenterology, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.
Li
You-Ming
YM
Xu
Guo-Qiang
GQ
D000328:Adult; D000368:Aged; D045744:Cell Line, Tumor; D005260:Female; D005500:Follow-Up Studies; D005753:Gastric Mucosa / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D053508:Matrix Metalloproteinase 12 / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000378:metabolism; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015996:Survival Rate
D012333:RNA, Messenger; D005982:Glutathione Transferase; C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12