Site-specific immobilization of CMP-sialic acid synthetase on magnetic nanoparticles and its use in the synthesis of CMP-sialic acid.
Through the native chemical ligation, CMP-sialic acid synthetase (CSS) was site-specifically immobilized on magnetic nanoparticles and presented excellent enzymatic performance.
Yu
Ching-Ching
CC
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Lin
Po-Chiao
PC
Lin
Chun-Cheng
CC
D002851:Chromatography, High Pressure Liquid; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000138:chemical synthesis*; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics; D007202:Indicators and Reagents; D008958:Models, Molecular; D000216:N-Acylneuraminate Cytidylyltransferase / Q000737:chemistry*; D053758:Nanoparticles; D038541:Neisseria meningitidis, Serogroup B / Q000201:enzymology
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D049452:Green Fluorescent Proteins; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D000216:N-Acylneuraminate Cytidylyltransferase; D004229:Dithiothreitol