Photoisomerization of the green fluorescence protein chromophore and the meta- and para-amino analogues.
The Z --> E photoisomerization and fluorescence quantum yields for the wild-type green fluorescence protein (GFP) chromophore (p-HBDI) and its meta- and para-amino analogues (m-ABDI and p-ABDI) in aprotic solvents (hexane, THF, and acetonitrile) and protic solvents (methanol and 10-20% H(2)O in THF) are reported. The dramatic decrease in the quantum yields on going from aprotic to protic solvents indicates the important role of solvent-solute hydrogen bonding in the nonradiative decay pathways. The enhanced fluorescence of m-ABDI is also discussed.
Yang
Jye-Shane
JS
Department of Chemistry, National Taiwan University, Taipei, Taiwan. jsyang@ntu.edu.tw
Huang
Guan-Jhih
GJ
Liu
Yi-Hung
YH
Peng
Shie-Ming
SM
D001593:Benzyl Compounds / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006860:Hydrogen Bonding; D048288:Imidazolines / Q000737:chemistry*; D007202:Indicators and Reagents; D007536:Isomerism; D010777:Photochemistry; D012997:Solvents; D013050:Spectrometry, Fluorescence
D001593:Benzyl Compounds; D048288:Imidazolines; D007202:Indicators and Reagents; D012997:Solvents; C529029:amino-p-hydroxybenzylideneimidazolinone; C529028:p-hydroxybenzylideneimidazolinone; D049452:Green Fluorescent Proteins