How to store autologous bone graft perioperatively: an in vitro study.
BACKGROUND
Autologous bone graft is the golden standard for bone grafting, but little is known about the influence of various preservation techniques used during surgery immediately following harvest on the osseous structures and graft vitality. Several studies focussed on the optimal treatment of the bone during harvest and implantation, but only few examined the intraoperative storage conditions on the back table. The aim of our study was to examine the influence of various intraoperative preservation techniques on human cancellous bone at different points to optimize the storage during surgery.


METHODS
Cancellous bone was harvested during hip arthroplasty and stored for 2 and 4 h under dry conditions, inside a swab moistened with saline solution or in saline solution, 5% glucose solution or culture medium. After the storage period, the bone was cultured and examined 7 days after the first cells grew out in one of these groups. Following the identification of the cells as osteoblast-like cells, the cultures were analysed by fluorescence staining, cell count and the XTT-test.


RESULTS
Fluorescence staining revealed no avital cells in all groups. Dry storage of the bone led to significantly lower cell metabolism after 2 h compared to saline solution and 5% glucose solution. The same was true after 4 h dry storage compared to the moistened swab, and glucose and culture medium. Cell count was significantly lower after 2 h of dry storage compared to saline solution and culture medium.


CONCLUSIONS
Perioperative storage of cancellous bone under dry conditions should be avoided. The bone graft should be stored in saline or 5% glucose solution or a moistened swab.
Maus
Uwe
U
Department of Orthopaedic Surgery, University Hospital of Aachen, Pauwelsstrasse 30, RWTH Aachen, Germany. umaus@ukaachen.de
Andereya
Stefan
S
Gravius
Sascha
S
Siebert
Christian Helge
CH
Schippmann
Thomas
T
Ohnsorge
Jörg Axel Karl
JA
Niedhart
Christopher
C
D000368:Aged; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005269:Femur / Q000166:cytology / Q000637:transplantation*; D005453:Fluorescence; D005947:Glucose; D006801:Humans; D008297:Male; D008875:Middle Aged; D019990:Perioperative Care; D012965:Sodium Chloride; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling; D014182:Transplantation, Autologous
D003470:Culture Media; D012965:Sodium Chloride; D005947:Glucose