Chemical synthesis and characterization of J46 peptide, an atypical class IIa bacteriocin from Lactococcus lactis subsp. cremoris J46 Strain.
Bacteriocin J46 is a 27-residue polypeptide produced by Lactococcus lactis subsp. cremoris J46 in fermented milk. The natural form of J46 (nJ46) exhibits a broad antimicrobial spectrum. Herein, we produced the synthetic form of J46 (sJ46) by solid-phase chemical synthesis. The biochemical and physico-chemical properties of sJ46, as well as its antimicrobial activity, were found to be identical to those of its natural counterpart nJ46. It showed a potent antimicrobial activity against both lactic acid bacteria and other Gram-positive microorganisms. (1)H-NMR conformational analysis of sJ46 indicates that it adopts a flexible random coil structure.
Lasta
Samar
S
Laboratoire de Biochimie, CNRS FRE 2738, Faculté de Médecine Nord, Bd Pierre Dramard, 13916 Marseille Cedex 20, France.
Fajloun
Ziad
Z
Darbon
Hervé
H
Mansuelle
Pascal
P
Andreotti
Nicolas
N
Sabatier
Jean-Marc
JM
Boudabous
Abdellatif
A
Sampieri
François
F
D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001430:Bacteriocins / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013294:Lactococcus lactis / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology; D008968:Molecular Conformation
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C530003:bacteriocin J46