Degradation of N-acyl homoserine lactone quorum sensing signal molecules by forest root-associated fungi.
A collection of mycorrhizal and nonmycorrhizal root-associated fungi coming from forest environments was screened for their ability to degrade N-acyl homoserine lactones (AHL) or to prevent AHL recognition by producing quorum sensing inhibitors (QSI). No production of QS-inhibitors or -activators was detected using the two biosensors Chromobacterium violaceum CV026 and Agrobacterium tumefaciens in the culture supernatant of these fungi. However, the ability to degrade C6- and 3O,C6-HSL was detected for three fungal isolates. Acidification assay revealed that the AHL were degraded by a lactonase activity for two of these isolates. These results demonstrated for the first time that the forest root-associated fungi are capable of degrading the AHL signal molecules.
Uroz
Stephane
S
INRA/UHP UMR 1136 Interactions Arbres Micro-organismes, Centre INRA de Nancy, Champenoux, France. uroz@nancy.inra.fr
Heinonsalo
Jussi
J
D054742:Acyl-Butyrolactones / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D005658:Fungi / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D053038:Quorum Sensing; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D012988:Soil Microbiology; D014197:Trees
D054742:Acyl-Butyrolactones; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase