title: Abrogation of anti-retinal autoimmunity in IL-10 transgenic mice due to reduced T cell priming and inhibition of disease effector mechanisms.
abstract: Experimental autoimmune uveitis (EAU) induced by immunization of animals with retinal Ags is a model for human uveitis. The immunosuppressive cytokine IL-10 regulates EAU susceptibility and may be a factor in genetic resistance to EAU. To further elucidate the regulatory role of endogenous IL-10 in the mouse model of EAU, we examined transgenic (Tg) mice expressing IL-10 either in activated T cells (inducible) or in macrophages (constitutive). These IL-10-Tg mice and non-Tg wild-type controls were immunized with a uveitogenic regimen of the retinal Ag interphotoreceptor retinoid-binding protein. Constitutive expression of IL-10 in macrophages abrogated disease and reduced Ag-specific immunological responses. These mice had detectable levels of IL-10 in sera and in ocular extracts. In contrast, expression of IL-10 in activated T cells only partially protected from EAU and marginally reduced Ag-specific responses. All IL-10-Tg lines showed suppression of Ag-specific effector cytokines. APC from Tg mice constitutively expressing IL-10 in macrophages exhibited decreased ability to prime naive T cells, however, Ag presentation to already primed T cells was not compromised. Importantly, IL-10-Tg mice that received interphotoreceptor retinoid-binding protein-specific uveitogenic T cells from wild-type donors were protected from EAU. We suggest that constitutively produced endogenous IL-10 ameliorates the development of EAU by suppressing de novo priming of Ag-specific T cells and inhibiting the recruitment and/or function of inflammatory leukocytes, rather than by inhibiting local Ag presentation within the eye.
authors.affiliation: The Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Agarwal
authors.forename: Reiko
authors.initials: R
authors.lastname: Horai
authors.forename: Angelia M
authors.initials: AM
authors.lastname: Viley
authors.forename: Phyllis B
authors.initials: PB
authors.lastname: Silver
authors.forename: Rafael S
authors.initials: RS
authors.lastname: Grajewski
authors.forename: Shao Bo
authors.initials: SB
authors.lastname: Su
authors.forename: Arrash T
authors.initials: AT
authors.lastname: Yazdani
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Mitchell
authors.initials: M
authors.lastname: Kronenberg
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Murray
authors.forename: Robert L
authors.initials: RL
authors.lastname: Rutschman
authors.forename: Chi-Chao
authors.initials: CC
authors.lastname: Chan
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Caspi
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D001327:Autoimmune Diseases / Q000276:immunology*; D015551:Autoimmunity; D004195:Disease Models, Animal; D005136:Eye Proteins / Q000276:immunology*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008285:Major Histocompatibility Complex / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012160:Retina / Q000276:immunology*; D012177:Retinol-Binding Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014605:Uveitis / Q000276:immunology* / Q000378:metabolism; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D005136:Eye Proteins; D016753:Interleukin-10; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial