Authentication of farmed and wild turbot (Psetta maxima) by fatty acid and isotopic analyses combined with chemometrics.
Fatty acid composition and stable isotope ratios of carbon (delta(13)C) and nitrogen (delta(15)N) were determined in muscle tissue of turbot (Psetta maxima). The multivariate analysis of the data was performed to evaluate their utility in discriminating wild and farmed fish. Wild (n=30) and farmed (n=30) turbot of different geographical origins (Denmark, The Netherlands, and Spain) were sampled from March 2006 to February 2007. The application of linear discriminant analysis (LDA) and soft independent modeling of class analogy (SIMCA) to analytical data demonstrated the combination of fatty acids and isotopic measurements to be a promising method to discriminate between wild and farmed fish and between wild fish of different geographical origin. In particular, IRMS (Isotope Ratio Mass Spectrometry) alone did not permit us to separate completely farmed from wild samples, resulting in some overlaps between Danish wild and Spanish farmed turbot. On the other hand, fatty acids alone differentiated between farmed and wild samples by 18:2n-6 but were not able to distinguish between the two groups of wild turbot. When applying LDA isotope ratios, 18:2n-6, 18:3n-3, and 20:4n-6 fatty acids were decisive to distinguish farmed from wild turbot of different geographical origin, while delta(15)N, 18:2n-6, and 20:1n-11 were chosen to classify wild samples from different fishing zones. In both cases, 18:2n-6 and delta(15)N were determinant for classification purposes. We would like to emphasize that IRMS produces rapid results and could be the most promising technique to distinguish wild fish of different origin. Similarly, fatty acid composition could be used to easily distinguish farmed from wild samples.
Busetto
Maria L
ML
University of Milan, Department of Veterinary Science & Technology for Food Safety, via Trentacoste 2, 20134 Milano, Italy.
Moretti
Vittorio M
VM
Moreno-Rojas
Jose M
JM
Caprino
Fabio
F
Giani
Ivan
I
Malandra
Renato
R
Bellagamba
Federica
F
Guillou
Claude
C
D000818:Animals; D002247:Carbon Isotopes / Q000032:analysis; D016002:Discriminant Analysis; D005227:Fatty Acids / Q000032:analysis*; D005398:Fisheries; D005412:Flatfishes / Q000145:classification*; D013058:Mass Spectrometry / Q000379:methods; D015233:Models, Statistical; D009132:Muscles / Q000737:chemistry*; D009587:Nitrogen Isotopes / Q000032:analysis
D002247:Carbon Isotopes; D005227:Fatty Acids; D009587:Nitrogen Isotopes