title: Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH.
abstract: Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase-domain fluorescence lifetime-based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24-well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell-based high-throughput screening applications.
authors.affiliation: University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, D-93040 Regensburg, Germany.
authors.forename: Anna S
authors.initials: AS
authors.lastname: Kocincová
authors.forename: Stefan
authors.initials: S
authors.lastname: Nagl
authors.forename: Sarina
authors.initials: S
authors.lastname: Arain
authors.forename: Christian
authors.initials: C
authors.lastname: Krause
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Borisov
authors.forename: Matthias
authors.initials: M
authors.lastname: Arnold
authors.forename: Otto S
authors.initials: OS
authors.lastname: Wolfbeis
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008904:Miniaturization; D055095:Optics and Photonics / Q000295:instrumentation*; D010100:Oxygen / Q000032:analysis*; D016958:Pseudomonas putida / Q000254:growth & development*
chemical_list: D010100:Oxygen