title: Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin.
abstract: An efficient and easy-to-execute single microbead SELEX approach is developed to generate high affinity ssDNA aptamers against botulinum neurotoxin.
authors.affiliation: Chemistry, Materials, Earth and Life Sciences Directorate, Lawrence Livermore National Laboratory, 7000 East Ave., Livermore, CA 94550, USA. tok2@llnl.gov
authors.forename: Jeffrey B-H
authors.initials: JB
authors.lastname: Tok
authors.forename: Nicholas O
authors.initials: NO
authors.lastname: Fischer
mesh_terms: D000595:Amino Acid Sequence; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001905:Botulinum Toxins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000037:antagonists & inhibitors* / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D009498:Neurotoxins; D001905:Botulinum Toxins