Blue native electrophoresis study on lipases.
We have developed a modified blue native polyacrylamide gel electrophoresis (PAGE) protocol that can overcome aggregation of lipases seen in native PAGE. We have shown that two lipases, Pseudomonas aeruginosa lipase and Candida rugosa lipase, which aggregate in the native gel, can be resolved using our protocol. Activity staining was done to test for the functionality of the two lipases.
Saminathan
M
M
Centre for Biotechnology, Anna University, Chennai 600025, India.
Muthukumaresan
K Thirumalai
KT
Rengarajan
Srinivas
S
Muthukrishnan
Nandhini
N
Gautam
Pennathur
P
D002175:Candida / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008049:Lipase / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D013194:Staining and Labeling
D008049:Lipase