Identification of the RNA N-glycosidase activity of ricin in castor bean extracts by an electrochemiluminescence-based assay.
A simple electrochemiluminescence-based assay for RNA N-glycosidase activity has been modified to permit its use with authentic extracts of Ricinus communis (castor beans) and Abrus precatorius (jequirity seeds)--the natural sources of ricin and abrin. Modifications include the addition of an RNase inactivator to the reaction mixture, elimination of a signal-enhancing monoclonal antibody, and optimization of the incubation temperature. Concurrent testing with two substrates provides a diagnostic tool enabling castor bean toxins to be differentiated from a larger selection of N-glycosidase toxins than was previously examined.
Keener
William K
WK
U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA. william.keener@us.army.mil
Rivera
Victor R
VR
Cho
Chung Y
CY
Hale
Martha L
ML
Garber
Eric A E
EA
Poli
Mark A
MA
D002367:Ricinus communis / Q000201:enzymology*; D004563:Electrochemistry; D004789:Enzyme Activation; D007201:Indicator Dilution Techniques; D008163:Luminescent Measurements / Q000379:methods*; D010936:Plant Extracts / Q000378:metabolism; D054788:Ribosome Inactivating Proteins / Q000032:analysis* / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism*
D010936:Plant Extracts; D012276:Ricin; D054788:Ribosome Inactivating Proteins