Development of chloride homeostasis in albino and pigmented rat visual cortex neurons.
Albinism has a profound effect on visual development and visual function. Pharmacologically significant alterations of the two most important chloride-transporters--KCC2 (outward transporter) and NKCC1 (inward transporter)--functions were found in albino visual cortex neurons, comprising a higher NKCC1 and a lower KCC2 action. In this study, we compare the early postnatal development of the reversal potential of gamma-aminobutyric acidAR-mediated currents in visual cortex neurons of albino and pigmented rats. At birth we found no differences. At the time of eye opening (second week postnatally) the reversal potential of gamma-aminobutyric acidAR-mediated currents is 15 mV more positive and intracellular Cl- concentration is higher in visual cortex neurons of albinos than of pigmented rats.
Diykov
Dmitry
D
International Graduate School of Neuroscience, General Zoology and Neurobiology, Ruhr University Bochum, Bochum, Germany.
Barmashenko
Gleb
G
Hoffmann
Klaus-Peter
KP
D000367:Age Factors; D000417:Albinism / Q000473:pathology*; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002712:Chlorides / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D006706:Homeostasis / Q000502:physiology*; D066298:In Vitro Techniques; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000528:radiation effects; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D051381:Rats; D020318:Rats, Long-Evans; D017208:Rats, Wistar; D014793:Visual Cortex / Q000166:cytology* / Q000254:growth & development
D002712:Chlorides; D018691:Excitatory Amino Acid Antagonists; D018756:GABA Antagonists