title: Identification of glutathione sulfotransferase-pi (GSTP1) as a new resveratrol targeting protein (RTP) and studies of resveratrol-responsive protein changes by resveratrol affinity chromatography.
abstract: BACKGROUND
  Resveratrol shows chemopreventive and other biological affects in in vitro and some animal studies. The bioactivities of resveratrol may be attributed to qualitative and quantitative differences in its cell-type-specific interaction and binding with its cellular targets, denoted as resveratrol targeting proteins (RTPs).
  
  
  METHODS
  To isolate RTPs, resveratrol was linked to epoxy-activated agarose generating an affinity platform to allow the isolation, purification, and characterization of distinct RTPs from cultured prostate cancer cell extracts.
  
  
  RESULTS
  Glutathione sulfotransferase-pi (GSTP1) and estrogen receptor-beta (ER-beta) were found to be new RTPs. Resveratrol affinity chromatography was shown to be an easy method for analyzing resveratrol-responsive protein changes in the androgen-dependent LNCaP cells.
  
  
  CONCLUSIONS
  Resveratrol affects cellular functions at multiple levels, ranging from interaction with detoxification enzymes, such as GSTP1 and transcription by targeting factors such as ER-beta.
authors.affiliation: Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
authors.forename: Tze-Chen
authors.initials: TC
authors.lastname: Hsieh
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Wang
authors.forename: Haiteng
authors.initials: H
authors.lastname: Deng
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity / Q000379:methods; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D011485:Protein Binding; D011808:Quinone Reductases / Q000737:chemistry / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D008024:Ligands; D013267:Stilbenes; D011808:Quinone Reductases; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D000077185:Resveratrol