title: RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation and decreases protein translation.
abstract: Methyltransferases play essential roles in modulating important cellular and metabolic processes. A mouse putative N6-DNA methyltransferase gene (GenBank No AY456393) is a novel gene named mN6amt1(mN6A1). To investigate its function in cell fate and protein translation, RNA interference (RNAi)-mediated knock-down method was established. Cell cycle analysis suggests that the cell proliferation decreases after RNAi with mN6A1. The expression plasmid of luciferase was used to detect protein translation, and the results showed that luciferase expression decreased after RNAi with mN6A1, whereas increased after over-expression of mN6A1 or/and eRF1. The binding between mN6A1 and eRF1 was identified by co-immunoprecipitation and pull-down experiments. It might be suggested that mN6A1 participates in protein translation through interaction with eRF1.
authors.affiliation: National Center of Human Stem Cell Research and Engineering, Institute of Human Reproduction and Stem Cell Engineering, Central South University, Changsha 410078, People's Republic China.
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Liu
authors.forename: Dongsong
authors.initials: D
authors.lastname: Nie
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Huang
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Lu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D015254:DNA Modification Methylases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000235:genetics*; D034622:RNA Interference; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
chemical_list: D010454:Peptide Termination Factors; D015254:DNA Modification Methylases; D008780:Methyltransferases; C511868:PRED28 protein, mouse; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)