title: [In vitro percutaneous absorption of chromium powder and the effect of skin cleanser].
abstract: Occupational chromium dermatitis occurs frequently among cement and metal workers, workers dealing with leather tanning and employees in the ceramic industry. The present study, using an in-vitro system, evaluated percutaneous absorption of chromium powder and the effect of rapid skin decontamination with a common detergent. Experiments were performed using the Franz diffusion cell method with human skin. Physiological solution was used as receiving phase and a suspension of chromium powder in synthetic sweat was used as donor phase. The tests were performed without or with decontamination using the cleanser 30 minutes after the start of exposure. The amount of chromium permeated through the skin was analysed by Inductively Coupled Plasma Atomic Emission Spectroscopy and Electro Thermal Atomic Absorption Spectroscopy. Speciation analysis and measurements of chromium skin content were also performed. We calculated a permeation flux of 0.843 +/- 0.25 ng cm(-2) h(-1) and a lag time of 1.1 +/- 0.7 h. The cleaning procedure significantly increased chromium skin content, whereas skin passage was not increased. These results showed that chromium powder can pass through the skin and that skin decontamination did not decrease skin absorption. Therefore, it is necessary to prevent skin contamination when using toxic agents.
authors.affiliation: Unità Clinica Operativa di Medicina del Lavoro, Università degli Studi di Trieste.
authors.forename: F
authors.initials: F
authors.lastname: D'Agostin
authors.forename: M
authors.initials: M
authors.lastname: Crosera
authors.forename: G
authors.initials: G
authors.lastname: Adami
authors.forename: A
authors.initials: A
authors.lastname: Malvestio
authors.forename: R
authors.initials: R
authors.lastname: Rosani
authors.forename: M
authors.initials: M
authors.lastname: Bovenzi
authors.forename: G
authors.initials: G
authors.lastname: Maina
authors.forename: F Larese
authors.initials: FL
authors.lastname: Filon
mesh_terms: D000042:Absorption; D002857:Chromium / Q000493:pharmacokinetics*; D003902:Detergents / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D011208:Powders; D012867:Skin / Q000378:metabolism*
chemical_list: D003902:Detergents; D011208:Powders; D002857:Chromium