title: Gene expression patterns in glucose-stimulated podocytes.
abstract: To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-gamma were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.
authors.affiliation: Department of Internal Medicine, Yonsei University College of Medicine, 134 Shinchon-Dong Seodaemoon-Gu, Seoul 120-752, Republic of Korea.
authors.forename: Seung Hyeok
authors.initials: SH
authors.lastname: Han
authors.forename: Sanghwa
authors.initials: S
authors.lastname: Yang
authors.forename: Dong Sub
authors.initials: DS
authors.lastname: Jung
authors.forename: Jin Ji
authors.initials: JJ
authors.lastname: Li
authors.forename: Jin Ju
authors.initials: JJ
authors.lastname: Kim
authors.forename: Seung Jae
authors.initials: SJ
authors.lastname: Kwak
authors.forename: Dong Ki
authors.initials: DK
authors.lastname: Kim
authors.forename: Sung Jin
authors.initials: SJ
authors.lastname: Moon
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Dae-Suk
authors.initials: DS
authors.lastname: Han
authors.forename: Shin-Wook
authors.initials: SW
authors.lastname: Kang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D050199:Podocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005947:Glucose