New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C.
We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-gamma and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-gamma are due to the difference in HCV strains, but not the cellular environment.
Mori
Kyoko
K
Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
Abe
Ken-Ichi
K
Dansako
Hiromichi
H
Ariumi
Yasuo
Y
Ikeda
Masanori
M
Kato
Nobuyuki
N
D044966:Anti-Retroviral Agents / Q000494:pharmacology*; D001681:Biological Assay; D002460:Cell Line; D002999:Clone Cells; D016679:Genome, Viral; D015698:Genomic Library; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012093:Replicon / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
D044966:Anti-Retroviral Agents; D012313:RNA; D007371:Interferon-gamma