title: Enzymatic hydrolysis of organic-core microcapsules to produce aqueous-core microcapsules.
abstract: This paper describes the development of a new method to obtain aqueous-core microcapsules from organic-core capsules. The direct production of microcapsules, using tripropionin as organic material, followed by the hydrolysis of the core by a lipase was investigated. The enzymatic study showed that the enzyme obeyed a Michaelis-Menten mechanism and conditions for optimal activity were pH 7.5, 25-37 degrees C and 0% NaCl. Under these conditions, incubation of tripropionin-alginate microcapsules in a buffer containing the enzyme successfully produced aqueous-core capsules with reduced accumulation of alginate in the core in approximately 3 h.
authors.affiliation: Laboratory of Biological and Chemical Engineering, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
authors.forename: Veronique
authors.initials: V
authors.lastname: Breguet
authors.forename: Vojislav
authors.initials: V
authors.lastname: Vojinovic
authors.forename: Urs
authors.initials: U
authors.lastname: Von Stockar
authors.forename: Ian W
authors.initials: IW
authors.lastname: Marison
mesh_terms: D000818:Animals; D016466:CHO Cells; D002175:Candida / Q000201:enzymology; D002214:Capsules; D049109:Cell Proliferation; D002625:Chemistry, Organic / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014280:Triglycerides / Q000737:chemistry
chemical_list: D002214:Capsules; D014280:Triglycerides; D008049:Lipase; C031627:tripropionin