HGF suppresses the production of collagen type III and alpha-SMA induced by TGF-beta1 in healing fibroblasts.
The aim of this study was to examine the effectiveness of HGF in blocking TGF-beta1-induced collagen III and alpha-smooth muscle actin (alpha-SMA) production in rat healing fibroblasts, fibroblasts were obtained from healing medial collateral ligament (MCL) injury. Cell culture was supplemented with 5 ng/ml of TGF-beta1 along with increasing doses of HGF (10-40 ng/ml). The productions of collagen III in supernatants culture were assayed by enzyme-linked immunosorbent assay. Expression of alpha-SMA was assessed by Western blot. Treatment with TGF-beta1 significantly stimulated collagen III and alpha-SMA production in healing fibroblasts. Remarkably, the addition of HGF reduced productions of all components induced by TGF-beta1 in a dose-dependent manner. This study shows that HGF antagonizes the action of TGF-beta1 effectively in cultured healing MCL injury fibroblasts. The results provide a cellular and molecular basis for HGF's acting as a therapeutic agent for MCL scar formation and poor healing.
Jiang
Dapeng
D
Department of Pediatric Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Jiang
Zhitao
Z
Han
Fuyou
F
Zhang
Yubo
Y
Li
Zhaozhu
Z
D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D024061:Collagen Type III / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D066298:In Vitro Techniques; D008022:Ligaments / Q000293:injuries; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D000199:Actins; D024061:Collagen Type III; D017228:Hepatocyte Growth Factor; D053773:Transforming Growth Factor beta1; C498862:Acta2 protein, rat