Label-free optical biosensing with slot-waveguides.
We demonstrate label-free molecule detection by using an integrated biosensor based on a Si(3)N(4)/SiO(2) slot-waveguide microring resonator. Bovine serum albumin (BSA) and anti-BSA molecular binding events on the sensor surface are monitored through the measurement of resonant wavelength shifts with varying biomolecule concentrations. The biosensor exhibited sensitivities of 1.8 and 3.2 nm/(ng/mm(2)) for the detection of anti-BSA and BSA, respectively. The estimated detection limits are 28 and 16 pg/mm(2) for anti-BSA and BSA, respectively, limited by wavelength resolution.
Barrios
Carlos A
CA
Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de madrid, ETSI Telecommunicación, Ciudad Universitaria s/n, Madrid, Spain. cbarrios@die.upm.es
Bañuls
María José
MJ
González-Pedro
Victoria
V
Gylfason
Kristinn B
KB
Sánchez
Benito
B
Griol
Amadeu
A
Maquieira
A
A
Sohlström
H
H
Holgado
M
M
Casquel
R
R
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004867:Equipment Design; D007834:Lasers; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
D012710:Serum Albumin, Bovine