[Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies].
OBJECTIVE
To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.


METHODS
The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control.


RESULTS
Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control.


CONCLUSIONS
Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.
Sun
Lai-Jing
LJ
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. sunlaijing@163.com
Xu
Ge-Lin
GL
Wang
Hua-Lin
HL
Wu
Jie
J
Hu
Zhi-Hong
ZH
Zhou
Yi-Wu
YW
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001921:Brain / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods; D006624:Hippocampus / Q000821:virology; D006801:Humans; D011818:Rabies / Q000175:diagnosis* / Q000821:virology; D011820:Rabies virus / Q000276:immunology; D051381:Rats; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014021:Tissue Preservation / Q000379:methods*
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic