title: Large expansion segments in 18S rDNA support a new sponge clade (Class Demospongiae, Order Haplosclerida).
abstract: Newly emerging molecular phylogenetic hypotheses involving the sponge Order Haplosclerida (Class Demospongiae) are far removed from traditional views on their classification using morphology. In the new grouping of marine haplosclerid taxa by molecular data all members of one highly supported clade were found to have three large indels in the 18S rRNA gene. These indels were not found in this gene in other marine haplosclerids or in any other demosponges analysed. These indels were found in the variable V4 and V7 region of the gene, had high GC contents and formed stable double stranded helices in the 18S rRNA secondary structure. These indels are very important synapomorphies, provide high support for an alternative taxonomic scheme and could help resolve the phylogeny of this order in conjunction with other phylogenetically informative characters.
authors.affiliation: Molecular Evolution and Systematics Laboratory, MRI, Zoology Department, National University of Ireland, Galway, Ireland.
authors.forename: Niamh E
authors.initials: NE
authors.lastname: Redmond
authors.forename: Grace P
authors.initials: GP
authors.lastname: McCormack
mesh_terms: D000818:Animals; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011161:Porifera / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S