Cleavage of factor XIII by human neutrophil elastase results in a novel active truncated form of factor XIII A subunit.
The first step in the activation of plasma factor XIII (FXIII) is the cleavage of R37-G38 bond in FXIII-A subunit (FXIII-A) by thrombin, which makes the subsequent formation of an active transglutaminase possible. No active truncated form of FXIII-A, other than G38-FXIII-A, has been identified. In contrast to thrombin, which has a preference toward arginine residues, human neutrophil elastase (HNE) cleaves peptide bonds at small side-chain aliphatic amino acids, preferably at valine. As there are several valine residues close to the thrombin cleavage-site, we tested if an active truncated FXIII-A was formed during fragmentation of FXIII by HNE. It was demonstrated by Western blotting and transglutaminase assay that HNE induced a limited cleavage of FXIII-A resulting in the activation of both plasma and cellular FXIII; the maximal transglutaminase activities were 52.5% and 67.4% of thrombin-activated FXIII, respectively. After the relatively rapid activation a much slower inactivation occurred. HNE-activated FXIII cross-linked fibrin gamma- and alpha-chains in the clot formed by batroxobin moojeni. MALDI-TOF analysis of the cleaved fragments and N-terminal Edman degradation of the truncated protein identified V39-N40 as the primary cleavage-site and N40-FXIII-A as the active form. No primary cleavage occurred at V34, V35, V47, V50 residues. FXIII-A V34L polymorphism, which increases the rate of FXIII-A cleavage by thrombin, was without effect on FXIII activation by HNE. Molecular modeling located the primary HNE cleavage-site in the middle of the flexible and accessible Q32-L45 loop and showed that other neighboring valine residues were in less favorable position.
Bagoly
Zsuzsa
Z
University of Debrecen, Medical and Health Science Center, Clinical Research Center, PO Box 40, H-4012 Debrecen, Hungary.
Fazakas
Ferenc
F
Komáromi
István
I
Haramura
Gizella
G
Tóth
Eszter
E
Muszbek
Lászlo
L
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D003432:Cross-Linking Reagents; D005176:Factor XIII / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D026122:Factor XIIIa / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005337:Fibrin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D019272:Leukocyte Elastase / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D021122:Protein Subunits
D003432:Cross-Linking Reagents; D010446:Peptide Fragments; D021122:Protein Subunits; D005337:Fibrin; D005176:Factor XIII; D026122:Factor XIIIa; D019272:Leukocyte Elastase