Distribution of adenosine receptors in human sclera fibroblasts.
OBJECTIVE
Systemic treatment with adenosine receptor antagonists has been reported to affect the biochemistry and ultrastructure of rabbit sclera. This study was conducted to determine whether adenosine receptors (ADORs) are present in human scleral fibroblasts (HSF).


METHODS
Primary HSF were cultured in vitro and identified with anti-vimentin, anti-keratin, anti-desmin, and anti-S-100 antibodies. Confocal fluorescence microscopy was used to study the distribution of ADORs in the HSF cell lines and in the frozen human scleral sections. ADOR protein expression in HSF and human sclera was confirmed by western blot analysis of cell lysates.


RESULTS
ADORs were expressed in both HSF and human sclera. This was confirmed by western blot. ADORA1 expression was concentrated in the nucleus. ADORA2A was concentrated mainly in one side of the cytoplasm, and ADORA2B was found both in the nucleus and the cytoplasm. ADORA3 was expressed weakly in the cytoplasm.


CONCLUSIONS
All four subtypes of ADOR were found in HSF and may play a role in scleral remodeling.
Cui
Dongmei
D
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
Trier
Klaus
K
Chen
Xiang
X
Zeng
Junwen
J
Yang
Xiao
X
Hu
Jianmin
J
Ge
Jian
J
D000328:Adult; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D018613:Microscopy, Confocal; D018047:Receptors, Purinergic P1 / Q000378:metabolism*; D012016:Reference Values; D012590:Sclera / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
D018047:Receptors, Purinergic P1