Chemoenzymatic synthesis of prodigiosin analogues--exploring the substrate specificity of PigC.
Analogues of prodigiosin, a tripyrrolic pigment produced by Serratia species with potent immunosuppressive and anticancer activities, have been produced by feeding synthetic analogues of the normal precursor MBC to mutants of Serratia sp. ATCC 39006 or to engineered strains of Escherichia coli; in this way it has been shown that the prodigiosin synthesising enzyme, PigC, has a relaxed substrate-specificity.
Chawrai
Suresh R
SR
University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge, UK CB2 1EW.
Williamson
Neil R
NR
Salmond
George P C
GP
Leeper
Finian J
FJ
D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000145:classification / Q000378:metabolism*; D015394:Molecular Structure; D009154:Mutation / Q000235:genetics; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D012705:Serratia / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity
D004798:Enzymes; D017261:Glycosylphosphatidylinositols; D011353:Prodigiosin