Adenovirus vector-mediated gene therapy using iodized oil esters for hepatocellular carcinoma in rats.
BACKGROUND
When gene therapy is performed for malignant tumors, gene transfer efficiency and selectivity are extremely important. The usefulness of gene therapy by intraarterial injection of an adenovirus vector with iodized oil esters (IOEs) for hepatocellular carcinoma (HCC) was studied.


METHODS
HCC was induced in rats with diethyl nitrosamine and phenobarbital, after which either adenovirus vector expressing the herpes simplex virus thymidine kinase (AxCAHSVtk) and IOEs or AxCAHSVtk alone was injected through the hepatic artery. On postoperative days 2, 4 and 6, gancyclovir was injected into the peritoneum; blood sampling was performed on day 7.


RESULTS
Aspartate aminotransferase and alanine aminotransferase levels in the AxCAHSVtk with IOEs group were lower than in the AxCAHSVtk alone group (p = 0.0274, p = 0.0323). However, the survival rate was not significantly different between groups (p = 0.7122).


CONCLUSIONS
Intra-arterial injection of an adenovirus vector with IOEs can result in cancer-selective but not effective gene therapy for HCC.
Shiba
Hiroaki
H
Department of Surgery, Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan. hs0817@jikei.ac.jp
Misawa
Takeyuki
T
Iida
Tomonori
T
Okamoto
Tomoyoshi
T
Futagawa
Yasuro
Y
Sakurai
Minori
M
Ohashi
Toya
T
Eto
Yoshikatsu
Y
Yanaga
Katsuhiko
K
D000256:Adenoviridae / Q000235:genetics; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002645:Chickens; D015774:Ganciclovir / Q000008:administration & dosage / Q000493:pharmacokinetics; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D006499:Hepatic Artery; D007269:Injections, Intra-Arterial; D007459:Iodized Oil / Q000008:administration & dosage*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000628:therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000096:biosynthesis / Q000235:genetics
D007459:Iodized Oil; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D013937:Thymidine Kinase; D015774:Ganciclovir