title: Photoisomerization of the green fluorescence protein chromophore and the meta- and para-amino analogues.
abstract: The Z --> E photoisomerization and fluorescence quantum yields for the wild-type green fluorescence protein (GFP) chromophore (p-HBDI) and its meta- and para-amino analogues (m-ABDI and p-ABDI) in aprotic solvents (hexane, THF, and acetonitrile) and protic solvents (methanol and 10-20% H(2)O in THF) are reported. The dramatic decrease in the quantum yields on going from aprotic to protic solvents indicates the important role of solvent-solute hydrogen bonding in the nonradiative decay pathways. The enhanced fluorescence of m-ABDI is also discussed.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei, Taiwan. jsyang@ntu.edu.tw
authors.forename: Jye-Shane
authors.initials: JS
authors.lastname: Yang
authors.forename: Guan-Jhih
authors.initials: GJ
authors.lastname: Huang
authors.forename: Yi-Hung
authors.initials: YH
authors.lastname: Liu
authors.forename: Shie-Ming
authors.initials: SM
authors.lastname: Peng
mesh_terms: D001593:Benzyl Compounds / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006860:Hydrogen Bonding; D048288:Imidazolines / Q000737:chemistry*; D007202:Indicators and Reagents; D007536:Isomerism; D010777:Photochemistry; D012997:Solvents; D013050:Spectrometry, Fluorescence
chemical_list: D001593:Benzyl Compounds; D048288:Imidazolines; D007202:Indicators and Reagents; D012997:Solvents; C529029:amino-p-hydroxybenzylideneimidazolinone; C529028:p-hydroxybenzylideneimidazolinone; D049452:Green Fluorescent Proteins