title: Oseltamivir enhances hippocampal network synchronization.
abstract: Oseltamivir, a widely used anti-influenza drug, inhibits virus neuraminidase. A mammalian homologue of this enzyme is expressed in the brain, yet the effect of oseltamivir on central neurons is largely unknown. Patch-clamp recordings ex vivo revealed that oseltamivir enhanced spike synchronization between hippocampal CA3 pyramidal cells. Time-lapse multineuron calcium imaging revealed that oseltamivir and its active metabolite evoked synchronized population bursts that recruited virtually all neurons in the network. This unique, so-far-unknown, event was attenuated by muscarinic receptor antagonist. Thus, oseltamivir is a useful tool for investigating a new aspect of neural circuit operation.
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Usami
authors.forename: Takuya
authors.initials: T
authors.lastname: Sasaki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Satoh
authors.forename: Takahiro
authors.initials: T
authors.lastname: Akiba
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yokoshima
authors.forename: Tohru
authors.initials: T
authors.lastname: Fukuyama
authors.forename: Kenzo
authors.initials: K
authors.lastname: Yamatsugu
authors.forename: Motomu
authors.initials: M
authors.lastname: Kanai
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Shibasaki
authors.forename: Norio
authors.initials: N
authors.lastname: Matsuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
mesh_terms: D000200:Action Potentials; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D020013:Calcium Signaling / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D008856:Microscopy, Fluorescence; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009415:Nerve Net / Q000187:drug effects* / Q000201:enzymology; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D053139:Oseltamivir / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D017966:Pyramidal Cells / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D018727:Muscarinic Antagonists; D053139:Oseltamivir; D009439:Neuraminidase