Separation of peptides by capillary electrophoresis.
Peptides are an important class of analytes in chemistry, biochemistry, and food chemistry as well as medical and pharmaceutical sciences. As a high-resolution technique, capillary electrophoresis (CE) is well suited for the analysis of polar compounds such as peptides. In addition, CE is orthogonal to high-performance liquid chromatography, as both techniques are based on different physico-chemical separation principles. For the successful development of peptide separations by CE, operational parameters including buffer pH, buffer concentration and buffer type, applied voltage, and capillary dimensions, as well as background electrolyte additives such as detergents, ion-pairing reagents, cyclodextrins, (poly)amines, soluble polymers, etc. must be considered and optimized.
Scriba
Gerhard K E
GK
Department of Pharmaceutical Chemistry, University of Jena, Jena, Germany.
Psurek
Arndt
A
D000595:Amino Acid Sequence; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D012822:Silicon Dioxide; D013237:Stereoisomerism; D013696:Temperature
D002021:Buffers; D010455:Peptides; D012822:Silicon Dioxide