Determination of bergamottin in human plasma after grapefruit juice ingestion by an UPLC/MS/MS method.
Bergamottin was identified as a cause of pharmacokinetic interaction with grapefruit juice intake and as a physiologically active substance involved in lipolysis. However, the quantification method on concentrations of bergamottin in systemic circulation has not been well established. The aim of this study was to develop a simple, sensitive and high-throughput determination system for bergamottin in human plasma using an ultra performance liquid chromatography (UPLC)-MS-MS method. The UPLC system equipped with a UPLC BEH C18 column (2.1 x 50 mm, 1.7 microm) and an ESI prove was appropriate for detection of bergamottin. As a result, a primary product ion (m/z = 203) from precursor ion of bergamottin (m/z = 339) was observed. Plasma from a human volunteer who consumed grapefruit juice one hour before the time of blood sampling, was measured with the UPLC/MS/MS system. The determination of plasma-bergamottin was performed with the highest sensitivity presently available. In conclusion, we succeeded high performance bergamottin-determination in human plasma after grapefruit juice ingestion. The procedure can be usefulto clarify pharmacokinetic and pharmacodynamic characteristics of bergamottin.
Uesawa
Y
Y
Clinical Pharmaceutics Laboratory, Department of Pharmaceutics, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. uesawa@my-pharm.ac.jp
Yamada
H
H
Mohri
K
K
D000328:Adult; D001628:Beverages; D002851:Chromatography, High Pressure Liquid; D032083:Citrus paradisi / Q000737:chemistry*; D018565:Food-Drug Interactions; D011564:Furocoumarins / Q000097:blood*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*; D012015:Reference Standards
D011564:Furocoumarins; D011838:Radiation-Sensitizing Agents; C068337:bergamottin