Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation.
Several meiosis-specific proteins of Schizosaccharomyces pombe play essential roles in meiotic progression. We report here that a novel meiosis-specific protein kinase, Mug27 (also known as Ppk35), is required for proper spore formation. This kinase is expressed by the mug27(+) gene, which is abruptly transcribed after horsetail movement. This transcription is maintained until the second meiotic division. Green fluorescent protein (GFP)-tagged Mug27 appears at the start of prometaphase I, localizes to the spindle pole body (SPB) and then translocates to the forespore membrane (FSM) at late anaphase II. In the mug27Delta strain, smaller spores are produced compared with those of the mug27(+) strain. Moreover, spore viability was reduced by half or more compared with that of the mug27(+) strain. The protein-kinase activity of Mug27 appears to be important for its function: the putative kinase-dead Mug27 mutant had similar phenotypes to mug27Delta. Our results here indicate that the Mug27 kinase localizes at the SPB and regulates FSM formation and sporulation.
Ohtaka
Ayami
A
Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, Suita City, Osaka, Japan.
Okuzaki
Daisuke
D
Nojima
Hiroshi
H
D000705:Anaphase; D002462:Cell Membrane / Q000201:enzymology; D017124:Conserved Sequence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008540:Meiosis; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000166:cytology* / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000201:enzymology; D013172:Spores, Fungal / Q000166:cytology / Q000201:enzymology* / Q000502:physiology*; D014617:Vacuoles / Q000201:enzymology
D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D049452:Green Fluorescent Proteins; D011494:Protein Kinases; C113816:Sid2 protein, S pombe; C530792:Mug27 protein, S pombe; D017346:Protein Serine-Threonine Kinases