title: IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
abstract: The inflammatory reaction in autoimmune polymyositis and rejection of transplanted myoblasts is characterized by mononuclear cell infiltration. In other settings monocytes are locally recruited by an IL-6-induced IL-8-to-MCP-1 switch. IL-6, upon binding to soluble gp80 (sIL-6R), can interact with membrane-bound ubiquitously expressed gp130 and activate virtually all cells (transsignaling). We found that human myoblasts could use transsignaling to produce IL-6, MCP-1 and ICAM-1; the addition of sIL-6R, binding to IL-1beta-induced IL-6, greatly increases IL-6 production. These in vitro data support the hypothesis that locally secreted IL-6 can target monocyte chemotaxis and leukocytes trafficking through an IL-6, MCP-1 and ICAM-1 modulation.
authors.affiliation: Institute of General Pathology, Università Cattolica, L.go F. Vito 1, 00168 Roma, Italy.
authors.forename: Mariapaola
authors.initials: M
authors.lastname: Marino
authors.forename: Flavia
authors.initials: F
authors.lastname: Scuderi
authors.forename: Carlo
authors.initials: C
authors.lastname: Provenzano
authors.forename: Jürgen
authors.initials: J
authors.lastname: Scheller
authors.forename: Stefan
authors.initials: S
authors.lastname: Rose-John
authors.forename: Emanuela
authors.initials: E
authors.lastname: Bartoccioni
mesh_terms: D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism* / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000166:cytology; D032446:Myoblasts / Q000187:drug effects*; D013997:Time Factors
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1