title: Inhibitory effects of Angelicae Gigantis Radix on osteoclast formation.
abstract: Angelicae Gigantis Radix (AGR) is one of the most widely used herbal medications. AGR is the dried root of Angelica gigas Nakai (Umbelliferae), which is known as Korean angelica. This study investigated the effects of AGR on osteoclast formation using primary bone marrow cells. TNF-alpha treatment increased tartrate-resistant acid phosphatase (Trap) positive cells and Trap activity in bone marrow cells. However, AGR significantly decreased both TNF-alpha-induced Trap positive cells and Trap activity. RT-PCR analyses revealed that AGR decreased mRNA levels of Trap and matrix metalloproteinase-9 in TNF-alpha-treated bone marrow cells. In addition, AGR decreased TNF-alpha-induced activation of NF-kappaB. These results suggest that AGR has an inhibitory effect on the formation of osteoclasts and its effect is partially related to the NF-kappaB pathway.
authors.affiliation: College of Oriental Medicine, Semyung University, Jechon 390-711, South Korea.
authors.forename: Jong-Seob
authors.initials: JS
authors.lastname: Kil
authors.forename: Myung-Gyou
authors.initials: MG
authors.lastname: Kim
authors.forename: Hye Mi
authors.initials: HM
authors.lastname: Choi
authors.forename: Jong-Pil
authors.initials: JP
authors.lastname: Lim
authors.forename: Yungmin
authors.initials: Y
authors.lastname: Boo
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Jeung-Beum
authors.initials: JB
authors.lastname: Kim
authors.forename: Hye Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Kang-Hyun
authors.initials: KH
authors.lastname: Leem
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics; D029969:Angelica / Q000737:chemistry*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071681:Tartrate-Resistant Acid Phosphatase; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; D020780:Matrix Metalloproteinase 9