Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH.
Non-invasive, simultaneous optical monitoring of oxygen and pH during bacterial cultivation in 24-well microplates is presented using an integrated dual sensor for dissolved oxygen and pH values. The dual sensor is based on oxygen-sensitive organosilica microparticles and pH-sensitive microbeads from a polymethacrylate derivative embedded into a polyurethane hydrogel. The readout is based on a phase-domain fluorescence lifetime-based method referred to as modified frequency domain dual lifetime referencing using a commercially available detector system for 24-well microplates. The sensor was used for monitoring the growth of Pseudomonas putida bacterial cultures. The method is suitable for parallelized, miniaturized bioprocessing, and cell-based high-throughput screening applications.
Kocincová
Anna S
AS
University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, D-93040 Regensburg, Germany.
Nagl
Stefan
S
Arain
Sarina
S
Krause
Christian
C
Borisov
Sergey M
SM
Arnold
Matthias
M
Wolfbeis
Otto S
OS
D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D008904:Miniaturization; D055095:Optics and Photonics / Q000295:instrumentation*; D010100:Oxygen / Q000032:analysis*; D016958:Pseudomonas putida / Q000254:growth & development*
D010100:Oxygen