Rehmannia inhibits adipocyte differentiation and adipogenesis.
Rehmannia glutinosa, a Traditional Chinese Medicine (TCM), has been used to increase physical strength. Here, we report that Rehmannia glutinosa extract (RE) inhibits adipocyte differentiation and adipogenesis. RE impairs differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. At the molecular level, treatment with RE inhibits expression of the key adipocyte differentiation regulator C/EBPbeta, as well as C/EBPalpha and the terminal marker protein 422/aP2, during differentiation of preadipocytes into adipocytes. Additionally, RE inhibits the mitotic clonal expansion (MCE) process of adipocyte differentiation, and RE prevents localization of C/EBPbeta to the centromeres. RE also prevents high fat diet (HFD) induced weight gain and adiposity in rats. Taken together, our results indicate that Rehmannia glutinosa extract inhibits preadipocyte differentiation and adipogenesis in cultured cells and in rodent models of obesity.
Jiang
Lin
L
The Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China.
Zhang
Nai-xian
NX
Mo
Wei
W
Wan
Rui
R
Ma
Chun-gu
CG
Li
Xi
X
Gu
Yin-liang
YL
Yang
Xin-ying
XY
Tang
Qi-qun
QQ
Song
Hou-yan
HY
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002503:Centromere / Q000737:chemistry / Q000378:metabolism; D004032:Diet; D004195:Disease Models, Animal; D005223:Fats / Q000008:administration & dosage / Q000009:adverse effects; D050556:Fatty Acid-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D009765:Obesity / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D022763:CCAAT-Enhancer-Binding Protein-alpha; D022782:CCAAT-Enhancer-Binding Protein-beta; C493411:Fabp4 protein, mouse; D005223:Fats; D050556:Fatty Acid-Binding Proteins; D010936:Plant Extracts