title: Lanostane-type triterpenoids from the sclerotia of Inonotus obliquus possessing anti-tumor promoting activity.
abstract: Two new lanostane-type triterpenoids, 1 and 2 besides two known lanostane-type triterpenoids, 3 and 4 were isolated from the sclerotia of Inonotus obliquus. Their structures were determined to be lanosta-8,23E-diene-3beta,22R,25-triol (1) and lanosta-7:9(11),23E-triene-3beta,22R,25-triol (2) by spectral data. These compounds were tested for their anti-tumor-promoting activity using a short-term in vitro assay for EBV-EA activation induced by TPA. Compounds 1, 2 and 4 were stronger than the positive control, oleanolic acid. The most abundant compound 4 was investigated for the inhibitory effect in a two-stage carcinogenesis test on mouse skin using DMBA as an initiator and TPA as a promoter. Compound 4 was found to exhibit the potent anti-tumor promoting activity in the in vivo carcinogenesis test.
authors.affiliation: Department of Medicinal Chemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Taji
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Shun-Ichi
authors.initials: S
authors.lastname: Wada
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakuma
authors.forename: Reiko
authors.initials: R
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D012878:Skin Neoplasms / Q000517:prevention & control; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000956:Antigens, Viral; D000970:Antineoplastic Agents; D014315:Triterpenes