MCM-2, Ki-67 and PCNA protein expressions in pT3G2 colorectal cancer indicated lymph node involvement.
BACKGROUND
The rate of cancer cell growth depends on proliferative activity and on the tumor cell death rate. The study objective was to assess the expressions of Ki-67, PCNA and MCM2 in colorectal cancer and to analyze the correlation between the expressions and chosen anatomo-clinical parameters (patient's age, tumor location, lymph node metastases).


METHODS
The study material included primary tumors pT3 G2 obtained from 55 patients with colorectal cancer. Immunohistochemical investigations were performed using monoclonal antibodies (Ki-67- monoclonal mouse (DAKO) Clone MIB1, PCNA-monoclonal mouse (DAKO) Clone PC10, MCM2-polyclonal goat (Santa Cruz Biotechnology) Sc-9839) directed against human protein Ki-67, PCNA and MCM2.


RESULTS
The expressions of Ki-67, PCNA and MCM2 were found to correlate with the presence of lymph node metastases, but not with patient age or tumor location. The expressions of these proteins in the main tumor mass correlated with each other in the majority of patients.


CONCLUSIONS
The findings allow the assumption that positive expressions of Ki-67, PCNA and MCM2 in the main tumor mass in pathological stage pT3 and degree of histological malignancy G2 may indicate lymph node involvement.
Guzińska-Ustymowicz
Katarzyna
K
Department of General Pathomorphology, Medical University of Bialystok, Poland.
Stepień
Ewa
E
Kemona
Andrzej
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018797:Cell Cycle Proteins / Q000096:biosynthesis*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000096:biosynthesis*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D009687:Nuclear Proteins / Q000096:biosynthesis*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*
D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2