title: The effect of propoxycaine.HCl on the physical properties of neuronal membranes.
abstract: Fluorescent probe techniques were used to evaluate the effect of propoxycaine.HCl on the physical properties (transbilayer asymmetric lateral and rotational mobilities, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortex. An experimental procedure was used based on selective quenching of both 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer (RET) from the tryptophans of membrane proteins to Py-3-Py. Propoxycaine.HCl increased the bulk lateral and rotational mobilities, and annular lipid fluidity in SPMVs lipid bilayers, and had a greater fluidizing effect on the inner monolayer than that of the outer monolayer. The magnitude of increasing effect on annular lipid fluidity in SPMVs lipid bilayer induced by propoxycaine.HCl was significantly far greater than magnitude of increasing effect of the drug on the lateral and rotational mobilities of SPMVs lipid bilayer. It also caused membrane proteins to cluster. These effects of propoxycaine.HCl on neuronal membranes may be responsible for some, though not all, of the local anesthetic actions of propoxycaine.HCl.
authors.affiliation: Department of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University, Busan 602-739, Republic of Korea.
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Kim
authors.forename: Hun
authors.initials: H
authors.lastname: Mun
authors.forename: Sung-Key
authors.initials: SK
authors.lastname: Lee
authors.forename: Jong-Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Jang-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Yi-Hoon
authors.initials: YH
authors.lastname: Park
authors.forename: Young-Chan
authors.initials: YC
authors.lastname: Jeon
authors.forename: Ung-Chan
authors.initials: UC
authors.lastname: Yoon
authors.forename: Moon-Kyung
authors.initials: MK
authors.lastname: Bae
authors.forename: Hye-Ock
authors.initials: HO
authors.lastname: Jang
authors.forename: W Gibson
authors.initials: WG
authors.lastname: Wood
authors.forename: Il
authors.initials: I
authors.lastname: Yun
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002540:Cerebral Cortex / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004735:Energy Transfer; D005456:Fluorescent Dyes; D008560:Membrane Fluidity / Q000187:drug effects* / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D011430:Propoxycaine / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013570:Synaptic Membranes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000779:Anesthetics, Local; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D011430:Propoxycaine