title: Generation and characterization of human monoclonal antibodies to G5, a linear neutralization epitope on glycoprotein of rabies virus, by phage display technology.
abstract: The aim of the present study was to discover distinct human MAbs to RV with high neutralizing potency and a broad neutralization spectrum. A phage display technology was used to produce human scFv to G5, a conserved linear neutralization epitope on Gp of RV. A phage display scFv library with 6 x 10(7) members was constructed and the phage-scFv with 'antigen-binding' activities were selected with synthetic peptide G5-24. The obtained scFv genes were cloned into pET22b(+)/BL21(DE3) and from this we prepared purified scFv fragments. The assay of the specificity characteristics and neutralization capacity showed that two distinct clones with new human immunoglobulin V genes can recognize G5 specifically as well as neutralize different RV strains. They have potential for inclusion in an antibodies combination aimed for use in rabies PEP.
authors.affiliation: Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin, China. xlzhao985@sohu.com
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Jiang
authors.forename: Guan
authors.initials: G
authors.lastname: Yang
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019151:Peptide Library; D011820:Rabies virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D010948:Viral Plaque Assay
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D007128:Immunoglobulin Fragments; D019151:Peptide Library; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus