title: Determination of analyte concentration using the residual solvent resonance in (1)H NMR spectroscopy.
abstract: An NMR protocol that uses the residual proton signal from DMSO -d(6) (i.e., DMSO -d(5)) to determine the concentration of an analyte in a NMR sample was developed. This technique provides an alternative method for determining the molar concentration of compounds in solution without prior knowledge of their molecular weight. The method is particularly useful when submilligram quantities of compound are to be analyzed and is applicable to a variety of different research areas such as compound management, and natural product, combinatorial, and medicinal chemistry.
authors.affiliation: Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, QLD, Australia. Greg.Pierens@cmr.uq.edu.au
authors.forename: Gregory K
authors.initials: GK
authors.lastname: Pierens
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Carroll
authors.forename: Rohan A
authors.initials: RA
authors.lastname: Davis
authors.forename: Meredith E
authors.initials: ME
authors.lastname: Palframan
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Quinn
mesh_terms: D001688:Biological Products / Q000032:analysis*; D002110:Caffeine / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure
chemical_list: D001688:Biological Products; D002110:Caffeine; D004121:Dimethyl Sulfoxide