Human heart LIM protein has transcription activation ability related to LIM domain 1.
Human heart LIM (hhLIM), a muscle-specific expressed transcriptional coactivator of cardiac hypertrophy related gene, stimulates transcriptional activation of the ANF gene promoter in H9C2 cells. We revealed that the N-terminal half of hhLIM mediated this activation, in which the LIM domain 1 and protein kinase C phosphorylation site are important, especially the LIM domain 1. Further, mutagenesis of the conserved Cys in the LIM domain 1 abolished its ability to activate cardiac hypertrophy. These findings suggest that hhLIM is a typical LIM family member with powerful transcription activation.
Zheng
Bin
B
Department of Biochemistry, Hebei Medical University, Shijiazhuang, China.
Han
Mei
M
Wen
Jin-Kun
JK
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D048429:Cell Size; D006801:Humans; D060588:LIM Domain Proteins; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D032383:Myocytes, Cardiac / Q000166:cytology; D017434:Protein Structure, Tertiary; D051381:Rats; D015534:Trans-Activators / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D060588:LIM Domain Proteins; D009124:Muscle Proteins; D015534:Trans-Activators; C090114:cysteine and glycine-rich protein 3