title: Bag culture: a method for root-root co-culture.
abstract: A method named "bag culture" was developed for coculturing of Linum persicum (section Syllinum) and L. austriacum (section Linum) hairy roots. For this propose L. austriacum and L. persicum hairy root cultures were established using Agrobacterium rhizogenes in McCown medium. L. persicum hairy roots in bags (1 mm2 mesh) were successfully grown together with L. austriacum hairy roots. The amounts of podophyllotoxin (PTOX) and 6-methoxypodophyllotoxin (MPTOX) produced by L. persicum hairy root cultures were detected using HPLC. The results indicated that the amounts of both lignans and growth indexes of the two hairy roots decreased, that may be partly due to a competition between the two types of culture in using precursors of biosynthetic metabolites and the amount of culture medium which is available for each hairy root. However, MPTOX (0.17 g/100 g DW) and PTOX (0.02 g/100 g DW) levels of the L. persicum single culture in bag were significantly higher than of the other cultures which may be due to the immobilization effect of the bag.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Research Center, Shiraz University of Medical Sciences and Health Services, P.O. Box 71345-1583, Shiraz, IR Iran. Mohaghegh@sums.ac.ir
authors.forename: Abdolali
authors.initials: A
authors.lastname: Mohagheghzadeh
authors.forename: Azra
authors.initials: A
authors.lastname: Gholami
authors.forename: Shiva
authors.initials: S
authors.lastname: Hemmati
authors.forename: Shadab
authors.initials: S
authors.lastname: Dehshahri
mesh_terms: D018920:Coculture Techniques / Q000379:methods*; D019597:Flax / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D011034:Podophyllotoxin / Q000378:metabolism
chemical_list: D011034:Podophyllotoxin