title: Phytate inhibits bovine pericardium calcification in vitro.
abstract: OBJECTIVE
  The present study examined the inhibitory effects of pyrophosphate, etidronate, and phytate on bovine pericardium calcification in vitro.
  
  
  METHODS
  Bovine pericardium was glutaraldehyde fixed and then placed in a flow chamber in the presence of a synthetic physiological fluid alone (control) or the fluid plus various concentrations of pyrophosphate, etidronate, or phytate. Following a 96-h incubation, fragments were removed and assayed for calcification by measuring calcium and phosphorus levels.
  
  
  RESULTS
  The data indicated that both pyrophosphate and etidronate at 1 mg/l (5.75 and 4.95 microM, respectively) inhibited bovine pericardium calcification, whereas neither agent had an effect at 0.5 mg/l (2.87 and 2.47 microM, respectively). Phytate was the most potent inhibitor of calcification, and the effects of this agent were apparent at levels as low as 0.25 mg/l (0.39 microM).
  
  
  CONCLUSIONS
  While pyrophosphate, etidronate, and phytate were all able to inhibit bovine pericardium calcification in vitro, phytate was found to be the most effective.
authors.affiliation: Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS), University of Balearic Islands, Palma of Mallorca, Spain. fgrases@uib.es
authors.forename: Félix
authors.initials: F
authors.lastname: Grases
authors.forename: Pilar
authors.initials: P
authors.lastname: Sanchis
authors.forename: Antonia
authors.initials: A
authors.lastname: Costa-Bauzá
authors.forename: Oriol
authors.initials: O
authors.lastname: Bonnin
authors.forename: Bernat
authors.initials: B
authors.lastname: Isern
authors.forename: Joan
authors.initials: J
authors.lastname: Perelló
authors.forename: Rafael M
authors.initials: RM
authors.lastname: Prieto
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002114:Calcinosis / Q000517:prevention & control*; D002118:Calcium / Q000032:analysis; D002417:Cattle; D011756:Diphosphates / Q000494:pharmacology; D012968:Etidronic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D010496:Pericardium / Q000737:chemistry / Q000187:drug effects*; D010758:Phosphorus / Q000032:analysis; D010833:Phytic Acid / Q000494:pharmacology*
chemical_list: D050071:Bone Density Conservation Agents; D011756:Diphosphates; D010758:Phosphorus; D010833:Phytic Acid; D012968:Etidronic Acid; D002118:Calcium