Enzymatic hydrolysis of organic-core microcapsules to produce aqueous-core microcapsules.
This paper describes the development of a new method to obtain aqueous-core microcapsules from organic-core capsules. The direct production of microcapsules, using tripropionin as organic material, followed by the hydrolysis of the core by a lipase was investigated. The enzymatic study showed that the enzyme obeyed a Michaelis-Menten mechanism and conditions for optimal activity were pH 7.5, 25-37 degrees C and 0% NaCl. Under these conditions, incubation of tripropionin-alginate microcapsules in a buffer containing the enzyme successfully produced aqueous-core capsules with reduced accumulation of alginate in the core in approximately 3 h.
Breguet
Veronique
V
Laboratory of Biological and Chemical Engineering, Ecole Polytechnique Fédérale de Lausanne, Switzerland.
Vojinovic
Vojislav
V
Von Stockar
Urs
U
Marison
Ian W
IW
D000818:Animals; D016466:CHO Cells; D002175:Candida / Q000201:enzymology; D002214:Capsules; D049109:Cell Proliferation; D002625:Chemistry, Organic / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014280:Triglycerides / Q000737:chemistry
D002214:Capsules; D014280:Triglycerides; D008049:Lipase; C031627:tripropionin