title: TLC of alkaloids on cyanopropyl bonded stationary phases. Part II. Connection with RP18 and silica plates.
abstract: Some standards of the alkaloids and synthetic or natural mixtures are separated by two-dimensional thin-layer chromatography (TLC) on different adsorbent layers. Normal- and reversed-phase systems are used to obtain significant differences in the separation selectivity. Optimization of the one-dimensional TLC separation of the alkaloids' standards is performed on cyanopropyl-silica, RP18W, and silica layers in various eluents containing (besides diluent and modifier) silanol blockers, such as diethyl amine or ammonia. The most selective systems are used for the separation of the alkaloids' mixtures by two-dimensional TLC with an adsorbent gradient method. The mixtures of alkaloids or plant extracts (Chelidonium majus, Fumaria officinalis, or Glaucium flavum) are chromatographed in system I; the plates are connected with the plate pre-coated with various adsorbent, and partly separated fractions are transferred to the second layer and developed in system II. CN-silica-RP18W and CN-silica-silica are used as the connected layers. The alkaloids are identified by R(F) values of standards, and the components of plant extracts are identified in both systems, and by the comparison of UV spectra obtained in diode array detector densitometry.
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin.
authors.forename: Anna
authors.initials: A
authors.lastname: Petruczynik
authors.forename: Monika
authors.initials: M
authors.lastname: Waksmundzka-Hajnos
authors.forename: Tomasz
authors.initials: T
authors.lastname: Plech
authors.forename: Tomasz
authors.initials: T
authors.lastname: Tuzimski
authors.forename: Michał Ł
authors.initials: MŁ
authors.lastname: Hajnos
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Jóźwiak
authors.forename: Maria
authors.initials: M
authors.lastname: Gadzikowska
authors.forename: Anna
authors.initials: A
authors.lastname: Rompała
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; D012822:Silicon Dioxide