Novel quantitative electrophoretic analysis of endotoxins on microchips.
A novel microchip electrophoresis method was developed and applied for sensitive detection and quantitative analysis of endotoxins extracted from Gram-negative bacteria. The method provides a fast and quantitative differentiation of smooth and rough endotoxins based on the solubilization and complexation of the lipopolysaccharides with dodecylsulfate, and then with a fluorescent dye. The migration of the complexes was followed by LIF detection. The novel method is able to replace the SDS-PAGE with the advantage of high speed and better sensitivity, and by avoiding the laborious gel-preparation and silver staining.
Kilár
Anikó
A
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary.
Farkas
Viktor
V
Kovács
Krisztina
K
Kocsis
Béla
B
Kilár
Ferenc
F
D046209:Electrophoresis, Microchip / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000032:analysis*
D004731:Endotoxins