In vitro study of the effects of cadmium on the activation of the estrogen response element using the YES screen.
Estrogenic potential of environmental samples is frequently assessed using receptor-based functional assays. Using the yeast estrogen screen (YES) developed by Routledge and Sumpter, we assessed the ability of cadmium to activate the estrogen receptor-mediated response. No induced transcriptional activity was observed with a range of CdCl2 concentrations (1 nm-1mM). But, when combining cadmium with the model compound 17beta-estradiol, cadmium was able to significantly potentiate the induced estrogenic response for concentrations ranging from 15 nM to 1 microM. A maximal effect was observed at 0.5 microM with a ten fold reduction of the 17beta-estradiol EC50.
Denier
Xavier
X
Laboratory of Ecotoxicology, University of Le Havre, BP 540, 76058 Le Havre, France. xavier.denier@univ-lehavre.fr
Couteau
Jérome
J
Baudrimont
Magalie
M
Hill
Elisabeth M
EM
Rotchell
Jeanette
J
Minier
Christophe
C
D002104:Cadmium / Q000494:pharmacology*; D004784:Environmental Monitoring / Q000379:methods*; D011960:Receptors, Estrogen / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics*; D014874:Water Pollutants, Chemical / Q000494:pharmacology; D015003:Yeasts / Q000187:drug effects* / Q000235:genetics*
D011960:Receptors, Estrogen; D014874:Water Pollutants, Chemical; D002104:Cadmium