Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China.
A birnavirus strain, Paralichthys olivaceus birnavirus (POBV), was isolated and characterized from cultured flounder in China, and its complete genomic sequence was subsequently determined. The virus could induce cytopathic effects (CPE) in four of seven fish cell lines and was resistant to chloroform, 5-iodo-2'-deoxyuridine, acid and alkaline pH, and heat treatment. Purified virus particles had a typical icosahedral shape, with a diameter of approximately 55-60 nm. The genomic segments A and B of POBV were 3,091 and 2,780 bp in length and shared many of the features of the members of the family Birnaviridae. Segment A contained two partially overlapping ORFs encoding a polyprotein, pVP2-VP4-VP3, and a nonstructural protein, VP5, while segment B had only one ORF encoding for the VP1, a viral RNA-dependent RNA polymerase (RdRp). This is the first report about a birnavirus strain from a new non-salmonid host in China and its complete genome sequence.
Zhao
Zhe
Z
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Sciences, 430072 Wuhan, China.
Ke
Fei
F
Li
Zhengqiu
Z
Gui
Jianfang
J
Zhang
Qiya
Q
D000818:Animals; D018059:Birnaviridae / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002460:Cell Line; D002681:China; D002725:Chloroform / Q000494:pharmacology; D024882:Drug Resistance, Viral; D005393:Fish Diseases / Q000821:virology*; D005398:Fisheries; D005432:Flounder / Q000821:virology*; D016679:Genome, Viral; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007065:Idoxuridine / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames
D002725:Chloroform; D007065:Idoxuridine