Effects of gibberellin A(3) on growth and microcystin production in Microcystis aeruginosa (cyanophyta).
Environmental factors that affect the growth and microcystin production of microcystis have received worldwide attention because of the hazards microcystin poses to environmental safety and public health. Nevertheless, the effects of organic anthropogenic pollution on microcystis are rarely discussed. Gibberellin A(3) (GA(3)) is a vegetable hormone widely used in agriculture and horticulture that can contaminate water as an anthropogenic pollutant. Because of its common occurrence, we studied the effects of GA(3) on growth and microcystin production of Microcystis aeruginosa (M. aeruginosa) PCC7806 with different concentrations (0.001-25mg/L) in batch culture. The control was obtained without gibberellin under the same culture conditions. Growth, estimated by dry weight and cell number, increased after the GA(3) treatment. GA(3) increased the amounts of chlorophyll a, phycocyanin and cellular-soluble protein in the cells of M. aeruginosa PCC7806, but decreased the accumulation of water-soluble carbohydrates. In addition, GA(3) was observed to affect nitrogen absorption of the test algae, but to have no effect on the absorption of phosphorus. The amount of microcystin measured by enzyme-linked immunosorbent assay (ELISA) increased in GA(3) treatment groups, but the stimulatory effects were different in different culture phases. It is suggested that GA(3) increases M. aeruginosa growth by stimulating its absorbance of nitrogen and increasing its ability to use carbohydrates, accordingly increasing cellular pigments and thus finally inducing accumulation of protein and microcystin.
Pan
Xiaojie
X
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province, PR China. panxj2008@163.com
Chang
Fengyi
F
Kang
Lijuan
L
Liu
Yongding
Y
Li
Genbao
G
Li
Dunhai
D
D050260:Carbohydrate Metabolism / Q000187:drug effects; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D004797:Enzyme-Linked Immunosorbent Assay; D005875:Gibberellins / Q000633:toxicity*; D052998:Microcystins / Q000096:biosynthesis*; D046931:Microcystis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D010798:Phycocyanin / Q000378:metabolism; D010937:Plant Growth Regulators / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D005875:Gibberellins; D052998:Microcystins; D010937:Plant Growth Regulators; D014874:Water Pollutants, Chemical; D010798:Phycocyanin; D002734:Chlorophyll; D010758:Phosphorus; C078588:microcystin; C007842:gibberellic acid; D009584:Nitrogen; D000077194:Chlorophyll A