Expression of glutamyl aminopeptidase by osteogenic induction in rat bone marrow stromal cells.
Glutamyl aminopeptidase (GluAP, EC 3.4.11.7, ENPEP) is a 130-kDa homodimeric zinc metallopeptidase which specifically cleaves the N-terminal glutamate or aspartate residue of peptidic substrates such as cholecystokinin-8 or angiotensin (Ang) II, in vitro. We used a DNA microarray hybridization (Genechip Rat Expression Array 230A, Affymetrix Inc., Santa Clara, CA, USA) to demonstrate that GluAP was upregulated in osteogenic induced rat bone marrow stromal cells (BMSCs). To compare the expression of GluAP in the osteogenic differentiation and non-osteogenic differentiation of rat BMSCs in vitro, the cells were osteogenic induced in vitro. We also performed an MTT assay, alkaline phosphatase assay, alizarin red staining, and an immunohistochemical analysis to determine the osteogenic differentiation of BMSCs. The expression of GluAP was examined by real-time polymerase chain reaction (PCR). The real-time PCR results showed that GluAP was upregulated in osteogenic differentiated BMSCs in vitro, suggesting that GluAP may be correlated with the osteogenic differentiation of BMSCs.
Wu
Yao
Y
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, 14 Section 3, Renminnan Road, Chengdu, Sichuan 610041, PR China.
Xiao
Jingang
J
Wu
Ling
L
Tian
Weidong
W
Liu
Lei
L
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D015870:Gene Expression; D043384:Glutamyl Aminopeptidase / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D015854:Up-Regulation
D003094:Collagen; D043384:Glutamyl Aminopeptidase