title: Expression of cytokine genes following pre- and post-hatch immunization of chickens with herpesvirus of turkeys.
abstract: Induction of immune response as characterised by expression of cytokine genes in the spleen following immunization of pre- and post-hatch chickens with herpesvirus of turkeys (HVT) vaccine was studied. The pattern of expression of IFN-gamma and IL-10 genes in pre-hatch immunized chickens was different from that observed in post-hatch HVT immunized chickens. This expression pattern of cytokine genes was associated with significantly higher HVT transcripts in pre-hatch immunized chickens than in post-hatch immunized chickens. In conclusion, HVT immunization in chickens, irrespective of the age of immunization, stimulates host response characterised by the expression of cytokine genes, such as IFN-gamma and IL-10 in the spleen. However, the age of immunization appears to influence the temporal pattern of IFN-gamma and IL-10 expression as well as replication of HVT.
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Mohamed Faizal
authors.initials: MF
authors.lastname: Abdul-Careem
authors.forename: D Bruce
authors.initials: DB
authors.lastname: Hunter
authors.forename: Melissa D
authors.initials: MD
authors.lastname: Lambourne
authors.forename: Leah R
authors.initials: LR
authors.lastname: Read
authors.forename: Payvand
authors.initials: P
authors.lastname: Parvizi
authors.forename: Shayan
authors.initials: S
authors.lastname: Sharif
mesh_terms: D000367:Age Factors; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D026002:Herpesvirus 1, Meleagrid / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D018793:Interleukin-13 / Q000096:biosynthesis / Q000235:genetics; D020382:Interleukin-18 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D013154:Spleen / Q000276:immunology
chemical_list: D016207:Cytokines; D018793:Interleukin-13; D020382:Interleukin-18; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma