Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.
We sequenced and characterized two novel invertebrate-type lysozymes from the mosquito Anopheles gambiae. Alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the Class Insecta. Expression profiles were examined for the two mosquito genes through semiquantitative and real-time PCR analysis. Lys i-1 transcripts were found in adult females in the fat body and Malpighian tubules, whereas Lys i-2 was detected only in fat bodies. Blood-feeding resulted in significantly increased transcript abundance for both genes in the midguts. Neither gene was upregulated following bacterial challenge.
Paskewitz
S M
SM
Department of Entomology, University of Wisconsin, Madison, Wisconsin, USA.
Li
B
B
Kajla
M K
MK
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D041981:Gastrointestinal Tract / Q000201:enzymology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049750:Genome, Insect / Q000235:genetics; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
D019476:Insect Proteins; D012333:RNA, Messenger; D009113:Muramidase