Identification of 3,4-didehydrorhodopin as major carotenoid in Rhodopseudomonas species.
Recently we isolated the purple photosynthetic bacterium, Rhodopseudomonas sp. Rits, which was phylogenetically related to Rhodopseudomonas (Rps.) palustris. In this study, the light-dependent and time-dependent changes in the carotenoid composition were investigated by HPLC analysis of extracts from the cultures. All seven carotenoids in the biosynthetic pathway from lycopene to spirilloxanthin were detected. Especially, 3,4-didehydrorhodopin, having twelve conjugated double bonds as well as one terminal hydroxy group, was isolated in a remarkably large amount and fully characterized for the first time. The biosynthetic intermediate was commonly found in the Rps. palustris strains (CGA009, Morita and NBRC100419).
Mizoguchi
Tadashi
T
Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, Japan.
Isaji
Megumi
M
Harada
Jiro
J
Tamiaki
Hitoshi
H
D002338:Carotenoids / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D050296:Microbial Viability; D015394:Molecular Structure; D012241:Rhodopseudomonas / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
C529420:3,4-didehydrorhodopin; D002338:Carotenoids