[Effect of simulating static magnetic field of magnetic attachment on osteoblastic morphology and surface ultrastructure].
OBJECTIVE
To investigate effects of the static magnetic field (SMF) generated by dental magnetic attachment on osteoblastic morphology and surface ultrastructure.


METHODS
The in vitro cultured rat osteoblasts were exposed continuously to 12.5 mT, 125 mT, and 250 mT static magnetic fields for 1, 3, 5, and 7 days. After exposed in SMF, osteoblasts were observed under a phase contrast microscope, and then HE stained and observed under a light microscope. In addition, the cells were observed under a scanning electron microscope (SEM).


RESULTS
By continuous exposure, the different intensities of SMF exposure did not change the vital osteoblast growth pattern or distribution. The SEM photos showed that there were certain changes in cellular microstructures for osteoblasts after exposed to 12.5 mT for 5 to 7 days, as well as 125 mT and 250 mT for 3 to 7 days. The more exposure time increased, the more microvesicles on the surfaces of cells were observed.


CONCLUSIONS
Continuous SMF-stimulation could not affect the shape, distribution, and growth pattern of osteoblasts. The SMF of magnetic attachments could lead to certain changes in surface ultrastructures of osteoblasts in this study.
Yao
Wei
W
Department of Prosthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Zhao
Yu
Y
Li
Bing-yan
BY
Chao
Yong-lie
YL
DU
Li
L
D000818:Animals; D002478:Cells, Cultured; D003834:Denture Precision Attachment; D004574:Electromagnetic Fields; D010006:Osteoblasts / Q000528:radiation effects* / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley