title: Gene expression profile analysis of the peripheral blood mononuclear cells from tolerant living-donor liver transplant recipients.
abstract: Induction of transplant tolerance is a clinically desirable goal. To provide unbiased insight into transplant tolerance, we analyzed gene expression profiling in peripheral blood mononuclear cells from recipients of living-donor liver transplants (LDLTs) who had retained an immune tolerance with a well-functioning graft for several years using cDNA microarray. The comparative analyses with nontransplanted normal healthy volunteers showed that the majority of reliable detected genes were similar, and 5.6% of the genes in the tested genome (of which 627 up-regulated and 90 down-regulated) were significantly regulated and specific to tolerant LDLT recipients, indicating a significant genetic feature for inducing and maintaining immune tolerance. Moreover, the expression of several selected genes was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction, which correlated to microarray data. Our data indicated that cDNA microarray technology was useful for this application and provided many informative insights into transplant tolerance mechanism.
authors.affiliation: Laboratory of Transplantation Immunology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kawasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Iwasaki
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koshiba
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fujino
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Hara
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kitazawa
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Kimura
authors.forename: Shinji
authors.initials: S
authors.lastname: Uemoto
authors.forename: Xiao-Kang
authors.initials: XK
authors.lastname: Li
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D001773:Blood Cells / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics* / Q000276:immunology; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D016031:Liver Transplantation / Q000276:immunology*; D019520:Living Donors; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis