F2-isoprostane, inflammation, cardiac function and oxygenation in the endotoxaemic pig.
Prostaglandins are profoundly involved in endotoxaemic shock. Twenty pigs were given endotoxin at various doses (0.063-16 microg kg(-1) h(-1)). Three non-endotoxaemic pigs served as controls. Two eicosanoids were measured in plasma (8-iso-PGF(2alpha), a free radical-mediated lipid peroxidation product, and 15-keto-dihydro-PGF(2alpha) a major metabolite of COX activity) and evaluated against the pathophysiological responses that occur during endotoxaemic shock. Endotoxin mediates an increase in both 8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha). An increase in the endotoxin dose induced significant log-linear responses in 8-iso-PGF(2alpha) and 15-keto-dihydro-PGF(2alpha). Oxidative injury correlated to the TNF-alpha, IL-6, reductions in cardiac performance and to oxygen delivery and utilisation. COX-mediated inflammatory responses correlated to TNF-alpha, IL-6 and to reductions in arterial oxygen tension. Thus, oxidative injury and COX-mediated inflammation play a central role in the manifestation of endotoxaemic shock. Furthermore, formation of these eicosanoids on endotoxin-mediated alterations in pulmonary hypertension, oxygen delivery and oxygen utilisation seems to be independent of the administered endotoxin dose.
Lipcsey
Miklós
M
Section of Anaesthesiology and Intensive Care, Department of Surgical Sciences, Uppsala University Hospital, 751 85 Uppsala, Sweden. mlipcsey@eml.cc
Söderberg
Ewa
E
Basu
Samar
S
Larsson
Anders
A
Sjölin
Jan
J
Aström
Mikael
M
Eriksson
Mats B
MB
D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D006321:Heart / Q000187:drug effects / Q000503:physiopathology*; D007249:Inflammation / Q000097:blood*; D018384:Oxidative Stress; D012772:Shock, Septic / Q000097:blood* / Q000503:physiopathology*; D034421:Sus scrofa; D013552:Swine
C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost