title: BRAF and K-RAS mutation in a Greek papillary and medullary thyroid carcinoma cohort.
abstract: BACKGROUND
  The genes RAS and BRAF have been shown to be frequently mutated in human thyroid carcinomas. The aim of this study was to genotype a cohort of 55 sporadic papillary thyroid carcinomas (PTC) and 44 sporadic medullary thyroid carcinomas (MTC) for the K-RAS codon 12 and BRAF codon 600 mutations.
  
  
  METHODS
  K-RAS and BRAF mutations were characterized by an enhanced polymerase chain reaction followed by restriction fragment length polymorphism analysis (PCR-RFLP).
  
  
  RESULTS
  The K-RAS codon 12 mutation was found in 54.5% of the PTC and 40.9% of the MTC cases tested. The BRAF V600E mutation was detected in 27.3% of the PTC and 68.2% of the MTC samples. No significant association between K-RAS and BRAF mutations and clinicopathological parameters was found.
  
  
  CONCLUSIONS
  These data indicate that K-RAS and BRAF mutations were a frequent genetic event in our samples of sporadic PTC and MTC.
authors.affiliation: Histopathology Department, Evgenidion Hospital, University of Athens, Athens, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Goutas
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Vlachodimitropoulos
authors.forename: Myrto
authors.initials: M
authors.lastname: Bouka
authors.forename: Andreas C
authors.initials: AC
authors.lastname: Lazaris
authors.forename: George
authors.initials: G
authors.lastname: Nasioulas
authors.forename: Maria
authors.initials: M
authors.lastname: Gazouli
mesh_terms: D000328:Adult; D018276:Carcinoma, Medullary / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D005260:Female; D011905:Genes, ras; D006115:Greece; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf