A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity.
Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger.
Yan
Fei
F
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130023, People's Republic of China.
Yang
Wen-Kui
WK
Li
Xin-Yang
XY
Lin
Ting-Ting
TT
Lun
Yan-Ni
YN
Lin
Feng
F
Lv
Shao-Wu
SW
Yan
Gang-Lin
GL
Liu
Jun-Qiu
JQ
Shen
Jia-Cong
JC
Mu
Ying
Y
Luo
Gui-Min
GM
D000818:Animals; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000235:genetics*; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D012549:Schistosoma japonicum / Q000201:enzymology* / Q000235:genetics; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000235:genetics
D015801:Helminth Proteins; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase