title: Multi-antigen vaccines based on complex adenovirus vectors induce protective immune responses against H5N1 avian influenza viruses.
abstract: There are legitimate concerns that the highly pathogenic H5N1 avian influenza virus could adapt for human-to-human transmission and cause a pandemic similar to the 1918 "Spanish flu" that killed 50 million people worldwide. We have developed pandemic influenza vaccines by incorporating multiple antigens from both avian and Spanish influenza viruses into complex recombinant adenovirus vectors. In vaccinated mice, these vaccines induced strong humoral and cellular immune responses against pandemic influenza virus antigens, and protected vaccinated mice against lethal H5N1 virus challenge. These results indicate that this multi-antigen, broadly protective vaccine may serve as a safer and more effective approach than traditional methods for development of a pandemic influenza vaccine.
authors.affiliation: Division of Biodefense Vaccines, GenPhar Inc., 600 Seacoast Parkway, Mount Pleasant, SC 29464, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Holman
authors.forename: Danher
authors.initials: D
authors.lastname: Wang
authors.forename: Nicholas U
authors.initials: NU
authors.lastname: Raja
authors.forename: Min
authors.initials: M
authors.lastname: Luo
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Moore
authors.forename: Jan
authors.initials: J
authors.lastname: Woraratanadharm
authors.forename: Nutan
authors.initials: N
authors.lastname: Mytle
authors.forename: John Y
authors.initials: JY
authors.lastname: Dong
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D001835:Body Weight; D005822:Genetic Vectors; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D008297:Male; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007252:Influenza Vaccines