Osteoblastic cell behavior on nanostructured metal implants.
OBJECTIVE
Surface modifications at the nanometric scale may promote protein adsorption, cell adhesion and thus favor the osseointegration of metal implants. The behavior of osteoblastic cells was studied on mirror-polished (Smooth-SS) and nanostructured (Nano-SS) stainless steel surfaces.


METHODS
The nanostructuration was obtained by anodization. The surfaces were characterized by scanning electron, atomic force microscopy (AFM) and X-ray photoelectron specytoscopy. Osteoblastic cells were cultured on these surfaces.


CONCLUSIONS
AFM showed a regular array of pores on the Nano-SS. Osteoblastic cells spread more rapidly on Nano-SS surfaces than on Smooth-SS surfaces. Cell viability was similar on Smooth-SS and Nano-SS. Alkaline phosphatase activity increased with culture time whatever the substrate but was better enhanced at 21 days on the Nano-SS than on the Smooth-SS.


CONCLUSIONS
This study shows that the nanostructuration of metal implants may improve the adhesion and differentiation of osteoblastic cells.
Le Guehennec
Laurent
L
INSERM U791, Laboratory for Osteo-Articular & Dental Tissue Engineering, Faculty of dDental Surgery, 44042 Nantes, France.
Martin
Frantz
F
Lopez-Heredia
Marco-Antonio
MA
Louarn
Guy
G
Amouriq
Yves
Y
Cousty
Jacques
J
Layrolle
Pierre
P
D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010012:Osteogenesis / Q000502:physiology*; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
D001672:Biocompatible Materials; D013193:Stainless Steel