NtGNL1 is involved in embryonic cell division patterning, root elongation, and pollen tube growth in tobacco.
The function of the ARF-GEF family has drawn great attention recently, especially GNOM and GNL1, owing to their important role in plant development. A homolog of GBF was identified in Nicotiana tabacum, named NtGNL1, which is ubiquitously expressed throughout the tobacco life cycle. In NtGNL1 RNAi plants, irregular orientation of cell division and asynchronous cell development during early embryogenesis disrupted the symmetry of the developing embryo. In addition, root growth in transgenic lines was significantly slower than that in wild-type plants, although the structure of the root tip was largely intact. Pollen germination and pollen tube growth were also inhibited in the transgenic lines, and the tip of the pollen tube presented various aberrant morphologies in one of the transgenic lines. The phenotypes of different NtGNL1 RNAi transgenic lines suggest that the NtGNL1 is likely to be involved not only in embryogenesis and postembryonic development, but also in sexual reproduction; thus, NtGNL1 may play multiple and critical roles in plant development.
Wang
Lu
L
Liao
Fang-Lei
FL
Zhu
Li
L
Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Peng
Xiong-Bo
XB
Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Sun
Meng-Xiang
MX
Key Laboratory of Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
D019521:Body Patterning / Q000235:genetics*; D020126:Brefeldin A / Q000494:pharmacology; D002455:Cell Division / Q000235:genetics*; D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D018525:Germination; D020662:Guanine Nucleotide Exchange Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000502:physiology*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D053205:Pollen Tube / Q000033:anatomy & histology / Q000187:drug effects / Q000254:growth & development; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D034622:RNA Interference; D012639:Seeds / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*
D020662:Guanine Nucleotide Exchange Factors; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D020126:Brefeldin A