Antitubercular triterpenes and phytosterols from Pandanus tectorius Soland. var. laevis.
Bioassay-guided chromatographic purification of the antitubercular chloroform extract of Pandanus tectorius Soland. var. laevis leaves afforded a new tirucallane-type triterpene, 24,24-dimethyl-5 beta-tirucall-9(11),25-dien-3-one (1), squalene and a mixture of the phytosterols stigmasterol and beta-sitosterol. Microplate Alamar Blue Assay (MABA) showed that 1 inhibited the growth of Mycobacterium tuberculosis H(37)Rv with a MIC of 64 microg/mL, while squalene and the sterol mixture have MICs of 100 and 128 microg/mL, respectively.
Tan
Mario A
MA
Research Center for the Natural Sciences, Graduate School and College of Science, University of Santo Tomas, España, 1015, Manila, Philippines.
Takayama
Hiromitsu
H
Aimi
Norio
N
Kitajima
Mariko
M
Franzblau
Scott G
SG
Nonato
Maribel G
MG
D000995:Antitubercular Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development; D031673:Pandanaceae / Q000737:chemistry*; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013185:Squalene / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013265:Stigmasterol / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
C529812:24,24-dimethyltirucall-9(11),25-dien-3-one; D000995:Antitubercular Agents; D010840:Phytosterols; D010936:Plant Extracts; D012855:Sitosterols; D014315:Triterpenes; C025473:gamma-sitosterol; D013185:Squalene; D013265:Stigmasterol