title: Flavobacterium anhuiense sp. nov., isolated from field soil.
abstract: A novel strain, D3T, isolated from a field-soil sample obtained from Anhui Province, PR China, was characterized taxonomically by using a polyphasic approach. The cells were Gram-negative, yellow-pigmented rods devoid of flagella, but showing gliding motility. The organism was able to grow at 5-37 degrees C and at pH 4.0-10.0. A comparative 16S rRNA gene sequence analysis indicated that strain D3T is a member of the genus Flavobacterium, sharing highest sequence similarity with the type strain of Flavobacterium defluvii (96.7 %). The major isoprenoid quinone was MK-6 and the predominant fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1 omega 7c and/or iso-C15 : 0 2-OH) and C16 : 0. The DNA G+C content was 31.4 mol%. On the basis of phylogenetic and phenotypic data, strain D3T represents a novel species within the genus Flavobacterium, for which the name Flavobacterium anhuiense sp. nov. is proposed. The type strain is D3T (=KCTC 22128T = CGMCC 1.6859T).
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Shou-Yun
authors.initials: SY
authors.lastname: Yang
authors.forename: Wei-Kai
authors.initials: WK
authors.lastname: Gao
authors.forename: Chong-Xing
authors.initials: CX
authors.lastname: Zhang
authors.forename: Ke-Yun
authors.initials: KY
authors.lastname: Zhang
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D002681:China; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity; D009626:Terminology as Topic; D013729:Terpenes / Q000032:analysis
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013729:Terpenes