Grafting BSA onto poly[(L-lactide)-co-carbonate] microspheres by click chemistry.
Model protein bovine serum albumin (BSA) was covalently grafted onto poly[(L-lactide)-co-carbonate] microsphere surfaces by "click chemistry." The grafting was confirmed by confocal laser scanning microscopy and X-ray photoelectron spectroscopy. The maximum amount of surface-grafted BSA was 45 mg x g(-1). The secondary structure of the grafted BSA was analyzed by FTIR and the results demonstrated that the grafting did not affect protein structure. This strategy can also be used on microspheres prepared from poly(L-lactide)/poly[(L-lactide)-co-carbonate] blend materials.
Han
Yadong
Y
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Shi
Quan
Q
Hu
Junli
J
Du
Qing
Q
Chen
Xuesi
X
Jing
Xiabin
X
D000818:Animals; D002417:Cattle; D008863:Microspheres; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D011091:Polyesters; D012710:Serum Albumin, Bovine