title: An SV40 VP1-derived epitope recognized by CD8+ T cells is naturally processed and presented by HLA-A*0201 and cross-reactive with human polyomavirus determinants.
abstract: The CD8+ T cell responses directed toward the VP1 antigens of human polyomaviruses JC and BK recently were shown to be cross-reactive. Two HLA-A0201-restricted determinants from each virus have been defined and include JCp100-108 (ILMWEAVTL) and BKp108-116 (LLMWEAVTV) as well as JCp36-44 (SITEVECFL) and BKp44-52 (AITEVECFL). We asked whether VP1 from the related SV40 contains similar HLA-A0201-restricted determinants. In this study, we demonstrate that CD8+ T cells specific for SV40 VP1 p110-118 (ILMWEAVTV), but not p46-54 (SFTEVECFL), can be induced in HLA-A0201-transgenic mice and that these CD8+ T cells cross-react with the corresponding determinants from JC and BK virus. The SV40 p110 determinant was found to be processed and presented in SV40-infected cells. These results indicate that the JCp36/BKp44 determinants are distinctive for the human polyomaviruses while the JCp100/BKp108/SVp110 determinants are shared by all three viruses, providing a target for CD8+ T cell cross-reactivity.
authors.affiliation: Department of Surgery, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
authors.forename: Hephzibah Rani S
authors.initials: HR
authors.lastname: Tagaram
authors.forename: Alan M
authors.initials: AM
authors.lastname: Watson
authors.forename: Francois A
authors.initials: FA
authors.lastname: Lemonnier
authors.forename: Kevin
authors.initials: K
authors.lastname: Staveley-O'Carroll
authors.forename: Satvir S
authors.initials: SS
authors.lastname: Tevethia
authors.forename: Todd D
authors.initials: TD
authors.lastname: Schell
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001739:BK Virus / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D003429:Cross Reactions; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D015234:HLA-A Antigens / Q000276:immunology*; D015789:HLA-A2 Antigen; D006801:Humans; D007577:JC Virus / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D013539:Simian virus 40 / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D018984:Epitopes, T-Lymphocyte; D015234:HLA-A Antigens; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; C062481:VP1 protein, polyomavirus