title: Immunolocalization of defensins and cathelicidin in human glands of Moll.
abstract: The human gland of Moll located at the margin of the eyelids is a specialized apocrine gland, the function of which is not exactly known. The presence of antimicrobial proteins was identified in this gland recently, suggesting a function in the external ocular defense barrier against pathogens. In this study, we have demonstrated beta-defensin-1, beta-defensin-2 and cathelicidin (LL-37) in the secretory endpieces of the glands of Moll using immunohistochemical methods. beta-Defensin-1, beta-defensin-2 and cathelicidin (LL-37) showed a weak to moderately intensive staining pattern. The strongest immunolocalization of beta-defensin-1 was observed in the apical protrusions of the gland, which could also be observed but to a lesser extent in the case of beta-defensin-2 and cathelicidin. In active glandular cells, a granular staining pattern could be observed. beta-Defensin-1 and beta-defensin-2 varied in staining intensities, and even within one section strongly and weakly stained cells can coexist side by side. Also cells that, according to morphological criteria, appeared to be inactive still had an apical beta-defensin-1 immunolabeling. We assume that beta-defensin-1, beta-defensin-2 and cathelicidin (LL-37) work together with other antimicrobial peptides and proteins to create a defensive barrier against microbial invasion at the ocular surface.
authors.affiliation: Institute of Anatomy, Ludwig-Maximilians-University of Munich, Pettenkoferstrasse 11, 80336 Munich, Germany. mechthild.stoeckelhuber@med.uni-muenchen.de
authors.forename: Mechthild
authors.initials: M
authors.lastname: Stoeckelhuber
authors.forename: Elisabeth M
authors.initials: EM
authors.lastname: Messmer
authors.forename: Christoph
authors.initials: C
authors.lastname: Schubert
authors.forename: Beate M
authors.initials: BM
authors.lastname: Stoeckelhuber
authors.forename: Claudia
authors.initials: C
authors.lastname: Koehler
authors.forename: Ulrich
authors.initials: U
authors.lastname: Welsch
authors.forename: Robert
authors.initials: R
authors.lastname: Bals
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000032:analysis*; D001050:Apocrine Glands / Q000033:anatomy & histology* / Q000166:cytology; D023082:Defensins / Q000032:analysis*; D005143:Eyelids / Q000033:anatomy & histology* / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D023082:Defensins; D008070:Lipopolysaccharides; D054804:Cathelicidins