Proteomic analysis of triclosan resistance in Salmonella enterica serovar Typhimurium.
OBJECTIVE
The aim of this study was to determine and compare the proteomes of three triclosan-resistant mutants of Salmonella enterica serovar Typhimurium in order to identify proteins involved in triclosan resistance.


METHODS
The proteomes of three distinct but isogenic triclosan-resistant mutants were determined using two-dimensional liquid chromatography mass separation. Bioinformatics was then used to identify and quantify tryptic peptides in order to determine protein expression.


RESULTS
Proteomic analysis of the triclosan-resistant mutants identified a common set of proteins involved in production of pyruvate or fatty acid with differential expression in all mutants, but also demonstrated specific patterns of expression associated with each phenotype.


CONCLUSIONS
These data show that triclosan resistance can occur via distinct pathways in Salmonella, and demonstrate a novel triclosan resistance network that is likely to have relevance to other pathogenic bacteria subject to triclosan exposure and may provide new targets for development of antimicrobial agents.
Webber
Mark A
MA
Antimicrobial Agents Research Group, Division of Immunity and Infection, The University of Birmingham, Edgbaston, Birmingham B152TT, UK. m.a.webber@bham.ac.uk
Coldham
Nick G
NG
Woodward
Martin J
MJ
Piddock
Laura J V
LJ
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D024881:Drug Resistance, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D012486:Salmonella typhimurium / Q000737:chemistry / Q000187:drug effects*; D014260:Triclosan / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D020543:Proteome; D014260:Triclosan