Treatment of intracerebral glioblastomas with G422 tumour cell vaccine in a mouse model.
The aim of this study was to develop a tumour vaccine with the ability to induce and expand higher affinity cytotoxic T lymphocytes and stimulate an effective antitumour immune response. The hypothesis tested was that G422 glioblastoma cells modified with B7-1 and interferon (IFN)-gamma genes could serve as a tumour vaccine. It was found that therapeutic subcutaneous immunizations with this tumour vaccine significantly induced a cytotoxic T-cell response and prolonged the survival of female Kuming mice with intracerebral G422 tumour isografts. The data collectively suggested that G422 glioblastoma cells genetically modified with B7-1 and IFN-gamma genes could serve as a tumour vaccine.
Ma
Y H
YH
Department of Neurosurgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Yu
J B
JB
Yao
H P
HP
Zhan
R Y
RY
Zheng
J S
JS
D000818:Animals; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D005909:Glioblastoma / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*; D016219:Immunotherapy, Adoptive / Q000379:methods; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D018122:B7-1 Antigen; D019496:Cancer Vaccines; D007371:Interferon-gamma