title: [Study on molecular epidemiology of people infected with human immunodeficiency virus-1 in Hubei province].
abstract: OBJECTIVE
  To study the distribution of human immunodeficiency virus-1 (HIV-1) genotypes in Hubei province.
  
  
  METHODS
  Epidemiological survey was carried out to HIV-1 carriers who were identified in Hubei province. HIV-1 env V3-V4, gag P17/24 and the first exon of tat region were amplified by nested-polymerase chain reaction(nPCR) .The sequences were determined, and phylogenetic analyses were then performed.
  
  
  RESULTS
  4 HIV-1 strains or circulating recombinant forms (CRFs) were identified in Hubei province with subtype B' the predominant which covered 5 kinds of populations including former blood donors, blood receivers, spouses of the infected people, sex workers and their clients, homosexuals, mainly distributed in the areas with many former blood donors. CRF08-BC and CRF01-AE were found distributed in economically more developed cities or southern area of the province, and the major transmission routes was through sexual contact. Only 1 patient, an injecting drug user, was identified having subtype C.
  
  
  CONCLUSIONS
  Subtype B' was the main epidemic subtypes in Hubei province while CRF08-BC, CRF01-AE and subtype C were also circulating in the province, indicating the transmission of the disease might to become more complex.
authors.affiliation: Hubei Provincal Center for Disease Control and Prevention, Wuhan 430079, China.
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Chu
authors.forename: Xian-feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Fa-xian
authors.initials: FX
authors.lastname: Zhan
authors.forename: Heng
authors.initials: H
authors.lastname: Tang
authors.forename: Hui-ping
authors.initials: HP
authors.lastname: Chen
authors.forename: Ting-hai
authors.initials: TH
authors.lastname: Peng
authors.forename: Zuo-jiong
authors.initials: ZJ
authors.lastname: Gong
mesh_terms: D002681:China / Q000453:epidemiology; D015658:HIV Infections / Q000453:epidemiology*; D015497:HIV-1 / Q000145:classification; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral