Energy metabolism of a unique acetic acid bacterium, Asaia bogorensis, that lacks ethanol oxidation activity.
Acetic acid bacteria (AAB) are known as a vinegar producer on account of their ability to accumulate a high concentration of acetic acid due to oxidative fermentation linking the ethanol oxidation respiratory chain. Reactions in oxidative fermentation cause poor growth because a large amount of the carbon source is oxidized incompletely and the harmful oxidized products are accumulated almost stoichiometrically in the culture medium during growth, but a newly identified AAB, Asaia, has shown unusual properties, including scanty acetic acid production and rapid growth, as compared with known AAB as Acetobacter, Gluconobacter, and Gluconacetobacter. To understand these unique properties of Asaia in more detail, the respiratory chain and energetics of this strain were investigated. It was found that Asaia lacks quinoprotein alcohol dehydrogenase, but has other sugar and sugar alcohol-oxidizing enzymes specific to the respiratory chain of Gluconobacter, especially quinoprotein glycerol dehydrogenase. It was also found that Asaia has a cyanide-sensitive cytochrome bo(3)-type ubiquinol oxidase as sole terminal oxidase in the respiratory chain, and that it exhibits a higher H(+)/O ratio.
Ano
Yoshitaka
Y
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan.
Toyama
Hirohide
H
Adachi
Osao
O
Matsushita
Kazunobu
K
D016947:Acetobacteraceae / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D003486:Cyanides / Q000378:metabolism; D004734:Energy Metabolism; D000431:Ethanol / Q000378:metabolism; D020576:Gluconobacter / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
D003486:Cyanides; D000431:Ethanol; D006859:Hydrogen; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); C432586:ubiquinol oxidase; D010100:Oxygen