title: Delay expression of limonoid UDP-glucosyltransferase makes delayed bitterness in citrus.
abstract: Genes encoding limonoid UDP-glucosyltransferase from albedo of six Citrus species with different levels of delayed bitterness are isolated and cloned in vector pTZ57R/T. Our results indicate that gene sequence of sweet lime (with intense juice delayed bitterness) have complete identity with Satsuma mandarin (without distinctive juice delayed bitterness). Also gene sequence of Marsh seedless grapefruit, local orange and Thompson navel orange (with mild juice delayed bitterness) have very similarity with Satsuma mandarin. On the other hand, this gene started to express 60, 120, and 210 days after full blooming in albedo of Satsuma mandarin, sweet oranges and sour orange, and both grapefruit and sweet lime, respectively. Expression pattern of limonoid glucosyltransferase gene in leaves was quite different with albedo. Thus, we supposed the delayed bitterness in this species was related to delay in expression of limonoid glucosyltransferase gene in albedo and lower limonoid glucoside accumulation in fruits.
authors.affiliation: Department of Biochemistry, Faculty of Basic Sciences, Tarbiat Modares University, Jalal Street, Tehran 14115-175, Iran.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Zaare-Nahandi
authors.forename: Saman
authors.initials: S
authors.lastname: Hosseinkhani
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Zamani
authors.forename: Asad
authors.initials: A
authors.lastname: Asadi-Abkenar
authors.forename: Reza
authors.initials: R
authors.lastname: Omidbaigi
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001628:Beverages; D002957:Citrus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development*; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D013649:Taste
chemical_list: D010940:Plant Proteins; D005964:Glucosyltransferases; C406404:limonoid GTase