title: [The cytogenetic features of Burkitt leukemia].
abstract: OBJECTIVE
  To evaluate the incidence of chromosomal abnormalities in Burkitt leukemia (BL).
  
  
  METHODS
  Conventional cytogenetics (CC) was carried out to detect the karyotypes. Meanwhile, three color interphase fluorescence in situ hybridization (FISH) was used to detect the t(8;14)(q24;q32) in 17 newly diagnosed BL.
  
  
  RESULTS
  The results showed that the incidence of chromosomal abnormalities was 41.2% (7/17) by CC technique, including one of each for simple t(8;14)(q24;q32), complex chromosomal abnormality containing t(8;14)(q24;q32), t(8;22)(q24;q11), the complex chromosomal abnormality of t(12;22)(?;?), t(2;13)(?;?) with + 12, and one with two marker chromosomes. FISH method detected eight cases of t(8;14)(q24;q32), including the two detected by CC technique. Five samples (5/8) showed 2A1G1O2F (two blue, one green, one orange and two yellow signals in interphase nuclei), while three samples (3/8) showed 2A1G1O1F(two blue, one green, one orange and one yellow signals in interphase nuclei).
  
  
  CONCLUSIONS
  Two different breakpoints have been identified on the c-Myc locus on 8q24. Interphase FISH was more sensitive in detecting t(8;14)(q24;q32), and it is an important complement to CC. It should be used as a routine method for diagnosis of BL.
authors.affiliation: Department of Hematology, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, 210029 P. R. China. xxy8848@hotmail.com
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Xie
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Hairong
authors.initials: H
authors.lastname: Qiu
mesh_terms: D000293:Adolescent; D000328:Adult; D002051:Burkitt Lymphoma / Q000235:genetics*; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D055815:Young Adult