Complexation study of midazolam hydrochloride with beta-cyclodextrin: NMR spectroscopic study in solution.
(1)H NMR spectroscopic study of midazolam hydrochloride (MDL), beta-cyclodextrin (beta-CD) and their mixtures confirmed the formation of beta-CD-MDL inclusion complex in aqueous solution. The stoichiometry of the complexes was determined by Scott's method to be 1:1, and the association constant (K(a)) was calculated to be 108 M(-1). It was confirmed on the basis of 2D ROESY spectral data that only a fluorine-substituted aromatic ring acted as guest in complexation. Most of the aromatic signals of MDL exhibited induced shift changes as well as splitting, in the presence of beta-CD, indicating chiral differentiation of MDL by beta-CD.
Ali
Syed Mashhood
SM
Department of Chemistry, Aligarh Muslim University, Aligarh-202 002, UP, India. smashhoodali@gmail.com
Upadhyay
Santosh Kumar
SK
D009682:Magnetic Resonance Spectroscopy; D008874:Midazolam / Q000737:chemistry*; D015394:Molecular Structure; D011522:Protons; D012995:Solubility; D012996:Solutions; D047392:beta-Cyclodextrins / Q000737:chemistry*
D011522:Protons; D012996:Solutions; D047392:beta-Cyclodextrins; D008874:Midazolam