title: Novel quantitative electrophoretic analysis of endotoxins on microchips.
abstract: A novel microchip electrophoresis method was developed and applied for sensitive detection and quantitative analysis of endotoxins extracted from Gram-negative bacteria. The method provides a fast and quantitative differentiation of smooth and rough endotoxins based on the solubilization and complexation of the lipopolysaccharides with dodecylsulfate, and then with a fluorescent dye. The migration of the complexes was followed by LIF detection. The novel method is able to replace the SDS-PAGE with the advantage of high speed and better sensitivity, and by avoiding the laborious gel-preparation and silver staining.
authors.affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Pécs, Hungary.
authors.forename: Anikó
authors.initials: A
authors.lastname: Kilár
authors.forename: Viktor
authors.initials: V
authors.lastname: Farkas
authors.forename: Krisztina
authors.initials: K
authors.lastname: Kovács
authors.forename: Béla
authors.initials: B
authors.lastname: Kocsis
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kilár
mesh_terms: D046209:Electrophoresis, Microchip / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000032:analysis*
chemical_list: D004731:Endotoxins