title: Simultaneous cell morphometry and refractive index measurement with dual-wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium.
abstract: Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelength DHM that allows us to separately measure both the RI and the cellular thickness by exploiting an enhanced dispersion of the perfusion medium achieved by the utilization of an extracellular dye. The two wavelengths are chosen in the vicinity of the absorption peak of the dye, where the absorption is accompanied by a significant variation of the RI as a function of the wavelength.
authors.affiliation: Ecole Polytechnique Federale de Lausanne, Brain Mind institute, Lausanne, Switzerland.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Rappaz
authors.forename: Florian
authors.initials: F
authors.lastname: Charrière
authors.forename: Christian
authors.initials: C
authors.lastname: Depeursinge
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Magistretti
authors.forename: Pierre
authors.initials: P
authors.lastname: Marquet
mesh_terms: D000042:Absorption; D000465:Algorithms; D004396:Coloring Agents / Q000494:pharmacology; D006696:Holography / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D015233:Models, Statistical; D055095:Optics and Photonics; D010477:Perfusion; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012568:Schizosaccharomyces / Q000378:metabolism; D012815:Signal Processing, Computer-Assisted
chemical_list: D004396:Coloring Agents