AGEs and glucose levels modulate type I and III procollagen mRNA synthesis in dermal fibroblasts cells culture.
In the dermis, fibroblasts play an important role in the turnover of the dermal extracellular matrix. Collagen I and III, the most important dermal proteins of the extracellular matrix, are progressively altered during ageing and diabetes. For mimicking diabetic conditions, the cultured human dermal fibroblasts were incubated with increasing amounts of AGE-modified BSA and D-glucose for 24 hours. The expression of procollagen alpha2(I) and procollagen alpha1(III) mRNA was analyzed by quantitative real-time PCR. Our data revealed that the treatment of fibroblasts with AGE-modified BSA upregulated the expression of procollagen alpha2(I) and procollagen alpha1(III) mRNA in a dose-dependent manner. High glucose levels mildly induced a profibrogenic pattern, increasing the procollagen alpha2(I) mRNA expression whereas there was a downregulation tendency of procollagen alpha1(III) mRNA.
Andreea
Serban Iren
SI
Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 105 Splaiul Independentei, 050097 Bucharest, Romania. irensro@yahoo.com
Marieta
Costache
C
Anca
Dinischiotu
D
D000328:Adult; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics*; D024061:Collagen Type III / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D024042:Collagen Type I; D024061:Collagen Type III; D017127:Glycation End Products, Advanced; D012333:RNA, Messenger; D005947:Glucose