Effect of curcumin supplementation on blood glucose, plasma insulin, and glucose homeostasis related enzyme activities in diabetic db/db mice.
We investigated the effect of curcumin on insulin resistance and glucose homeostasis in male C57BL/KsJ-db/db mice and their age-matched lean non-diabetic db/+ mice. Both db/+ and db/db mice were fed with or without curcumin (0.02%, wt/wt) for 6 wks. Curcumin significantly lowered blood glucose and HbA 1c levels, and it suppressed body weight loss in db/db mice. Curcumin improved homeostasis model assessment of insulin resistance and glucose tolerance, and elevated the plasma insulin level in db/db mice. Hepatic glucokinase activity was significantly higher in the curcumin-supplemented db/db group than in the db/db group, whereas glucose-6-phosphatase and phosphoenolpyruvate carboxykinase activities were significantly lower. In db/db mice, curcumin significantly lowered the hepatic activities of fatty acid synthase, beta-oxidation, 3-hydroxy-3-methylglutaryl coenzyme reductase, and acyl-CoA: cholesterol acyltransferase. Curcumin significantly lowered plasma free fatty acid, cholesterol, and triglyceride concentrations and increased the hepatic glycogen and skeletal muscle lipoprotein lipase in db/db mice. Curcumin normalized erythrocyte and hepatic antioxidant enzyme activities (superoxide dismutase, catalase, gluthathione peroxidase) in db/db mice that resulted in a significant reduction in lipid peroxidation. However, curcumin showed no effect on the blood glucose, plasma insulin, and glucose regulating enzyme activities in db/+ mice. These results suggest that curcumin seemed to be a potential glucose-lowering agent and antioxidant in type 2 diabetic db/db mice, but had no affect in non-diabetic db/+ mice.
Seo
Kwon-Il
KI
Department of Food and Nutrition, Sunchon National University, Jeonnam, Republic of Korea.
Choi
Myung-Sook
MS
Jung
Un Ju
UJ
Kim
Hye-Jin
HJ
Yeo
Jiyoung
J
Jeon
Seon-Min
SM
Lee
Mi-Kyung
MK
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism*; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000097:blood / Q000188:drug therapy*; D019587:Dietary Supplements; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D006442:Glycated Hemoglobin / Q000187:drug effects / Q000378:metabolism; D006706:Homeostasis; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008071:Lipoprotein Lipase / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology
D001786:Blood Glucose; D003474:Curcumin; D005947:Glucose; D006442:Glycated Hemoglobin; D007328:Insulin; D008071:Lipoprotein Lipase