The effects of pyrene exposure on exercise performance, muscle contraction, and mitochondrial O(2) consumption in the leopard frog (Rana pipiens).
The present study exposed frogs to the PAH pyrene and measured exercise performance, muscle contractile ability, mitochondrial O(2) consumption, and membrane potential. Leopard frogs, Rana pipiens, were exposed for seven days in control or pyrene saturated water aquaria. Frogs were randomly placed into one of four groups: (i) pyrene-exposed exercised, (ii) control, exercised, (iii) pyrene-exposed, non-exercised, and (iv) control, non-exercised. Following the acute exposure, exercise duration, muscle contractile ability, blood gases and pH, glycogen levels, crossbridge formation and contractile length, and mitochondrial respiration were measured. The data revealed that pyrene exposure produced many adverse effects in Leopard frogs including significant reductions in exercise performance, muscle contractile ability, and alterations to muscle mitochondrial O(2) consumption and membrane potential. These data suggest that PAH exposure may limit survival for frogs in the wild by limiting foraging, mating, and predator avoidance behaviors.
Stabenau
Erich K
EK
Department of Biology, Bradley University, Peoria, Illinois 61625, USA. eks@bradley.edu
Sasser
Anna
A
Schulte
Crystal
C
D000818:Animals; D001784:Blood Gas Analysis; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D011721:Pyrenes / Q000633:toxicity*; D011894:Rana pipiens
D011721:Pyrenes; C030984:pyrene; D010100:Oxygen