Comparative examination and validation of ELISA test systems for Salmonella typhimurium diagnosis of slaughtering pigs.
The most frequently isolated Salmonella serotype from pork in Germany is S. typhimurium, especially phagetype DT 104. The monitoring programs on Salmonella in swine are based on enzyme-linked immunoadsorbent assay (ELISA) detecting antibodies in serum or meat juice. These serological results are used to classify swine herds in three categories to assess the hygienic status of farm regarding Salmonella infection in pigs. The object of this study was the comparative evaluation of four indirect Salmonella ELISA tests approved in Germany to detect Salmonella typhimurium infection of swine. Three tests (A-C) are based on LPS-antigen and directed against specific IgG-antibodies. The fourth test (D) bases on a whole-cell-lysate antigen and discriminates between Salmonella specific IgA-, IgM- and IgG-antibodies. In a longitudinal study sixteen 6 weeks old weaning pigs were orally infected with S. typhimurium DT 104. During an observation period of 138d clinical and bacteriological parameters were monitored and serum samples obtained at regular intervals as well as meat juice samples taken at slaughter were examined by the respective ELISA systems. Study results reveal that all tested ELISA systems are able to detect S. typhimurium infection in pigs in both sample matrices, blood serum and meat juice whereas test D showed the highest sensitivity to detect Salmonella antibodies in pigs. The sensitivity to detect Salmonella antibodies varied between tests A and C according to the used cut-off (test specific cut-off vs. recommended surveillance cut-off) resulting in a change of seroprevalence and hence may influence the Salmonella status of the farm.
Szabó
I
I
Federal Institute for Risk Assessment, Division of Biological Safety, Thielallee 88-92, 14195 Berlin, Germany. Istvan.Szabo@bfr.bund.de
Scherer
K
K
Roesler
U
U
Appel
B
B
Nöckler
K
K
Hensel
A
A
D000003:Abattoirs; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D005506:Food Contamination / Q000517:prevention & control; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D008070:Lipopolysaccharides / Q000276:immunology*; D015203:Reproducibility of Results; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012486:Salmonella typhimurium / Q000276:immunology*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides