title: [Amniotic membrane for ocular surface reconstruction after conjunctival squamous cell carcinoma resection].
abstract: OBJECTIVE
  This study was designed to evaluate the use of human amniotic membrane for ocular surface reconstruction after conjunctival squamous cell carcinoma resection.
  
  
  METHODS
  Amniotic membrane was obtained at the time of cesarean section and was preserved at -80 masculineC in glycerol and cornea culture media at a ratio of 1:1. The inclusion criteria were patients presenting proliferating lesions suggestive of squamous cell carcinoma (flat or elevated white lesions resembling "fish meat") that involve the conjunctiva, limbus and cornea. Eight eyes of 8 patients with conjunctival "squamous cell carcinoma" underwent tumor resection with amniotic membrane transplantation. Three of these cases underwent total corneal epitheliectomy and amniotic membrane transplantation associated with limbal autograft.
  
  
  RESULTS
  Mean follow-up time was 17.8 months (range, 10-35 months). In four patients (71.4%) surgical treatment was successful, with good ocular surface stability. In two patients (28.6%) results were partially successful, with mild cicatricial alterations. One patient was excluded from the study due to aggressive tumor recurrence with intraocular invasion that needed to be removed with exenteration.
  
  
  CONCLUSIONS
  This study suggests that amniotic membrane transplantation is a good alternative for ocular surface reconstruction after conjunctival squamous cell carcinoma resection.
authors.affiliation: Setor de Córnea e Doenças Externas, Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, SP, Brasil. paulo.r.carvalho@globo.com
authors.forename: Paulo Roberto de
authors.initials: PR
authors.lastname: Carvalho-Rêgo
authors.forename: José Alvaro Pereira
authors.initials: JA
authors.lastname: Gomes
authors.forename: Priscila Luppi
authors.initials: PL
authors.lastname: Ballalai
authors.forename: Marcelo Carvalho
authors.initials: MC
authors.lastname: Cunha
authors.forename: Luciene Barbosa de
authors.initials: LB
authors.lastname: Sousa
authors.forename: Clélia Maria
authors.initials: CM
authors.lastname: Erwenne
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000650:Amnion / Q000637:transplantation*; D002294:Carcinoma, Squamous Cell / Q000601:surgery*; D003230:Conjunctival Neoplasms / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D016896:Treatment Outcome; D055815:Young Adult