Aerosol delivery in ventilated newborn pigs: an MRI evaluation.
Pulmonary deposition of inhaled drugs in ventilated neonates has not been studied in vivo. The objective of this study was to evaluate pulmonary delivery of gadopentetate dimeglumine (Gd-DTPA) following nebulization in ventilated piglets using magnetic resonance imaging. Seven ventilated piglets (5 +/- 2 d old, weight 1.8 +/- 0.5 kg) were scanned in the Bruker/Siemens 4T magnetic resonance scanner using T1 weighted spin-echo sequence. Aerosols of Gd-DTPA were generated continuously using the MiniHeart jet nebulizer. Breath-hold coronal images were obtained before and every 10 min during aerosolized Gd-DTPA for 90 min. Signal intensity (SI) changes over the lungs, kidneys, liver, skeletal muscle, and heart were evaluated. A significant increase in SI was observed in the lungs, kidney, and liver at 10, 20, and 40 min respectively after start of aerosol. At the end of 90 min, the SI increased by 95%, 101%, and 426% over the right lung, left lung, and kidney, respectively. A much smaller increase in SI was observed over the liver. In conclusion, we have demonstrated effective pulmonary aerosol delivery within 10 min of contrast nebulization in ventilated piglets. Contrast visualization in the kidneys within 20 min of aerosol initiation reflects alveolar absorption, glomerular filtration and renal concentration.
Sood
Beena G
BG
Department of Pediatrics, Children's Hospital of Michigan, 4H42, Detroit, MI 48201, USA. bsood@med.wayne.edu
Shen
Yimin
Y
Latif
Zahid
Z
Chen
Xinguang
X
Sharp
Jody
J
Neelavalli
Jaladhar
J
Joshi
Aparna
A
Slovis
Thomas L
TL
Haacke
E M
EM
D000042:Absorption / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D003287:Contrast Media / Q000008:administration & dosage* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000295:instrumentation* / Q000379:methods; D019786:Gadolinium DTPA / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005919:Glomerular Filtration Rate / Q000502:physiology; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism; D008168:Lung / Q000033:anatomy & histology / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism; D009330:Nebulizers and Vaporizers; D013552:Swine; D012122:Ventilators, Mechanical
D003287:Contrast Media; D019786:Gadolinium DTPA