Proteomic analysis on a high salt tolerance introgression strain of Triticum aestivum/Thinopyrum ponticum.
Soil salinity is a major abiotic constraint to agricultural productivity. We successfully bred a new common wheat (Triticum aestivum L.) introgression variety (Shanrong No. 3) with high salt-tolerance via asymmetric somatic hybridization between common wheat cultivar (Jinan 177) and UV-irradiated Agropyron elongatum (Thinopyrum ponticum Podp). We report here a comparative proteomic analysis to investigate variety-specific and salt-responsive proteins between seedling-roots of Shanrong No. 3 and Jinan 177. In total, 114 spots reproducibly presented differential expression patterns on 2-DE maps. Of them, 34 were variety-specific and 49 were salt-responsive. We identified 110 spots by MALDI-TOF MS and partially confirmed by MALDI-TOF-TOF MS, and functionally classified them into signal transduction, transcription and translation, transporting, chaperones, proteolysis and detoxification, etc. Meanwhile, we also found the alteration of protein expression of Shanrong No. 3 through inhibition of old proteins and production of novel ones, change in abundance and sensitivity of some nonsalt-responsive and salt-responsive proteins, as well as PTMs. Furthermore, comparison between proteome and transcripteome using cDNA microarray showed that there were only 20 proteins with abundances correlative to signal densities of corresponding EST probes. This study gives us a global insight into proteomic difference between Shanrong No. 3 and Jinan 177 in constitute and to salt-response.
Wang
Meng-Cheng
MC
School of Life Sciences, Shandong University, Jinan, PR China.
Peng
Zhen-Ying
ZY
Li
Cui-Ling
CL
Li
Fei
F
Liu
Chun
C
Xia
Guang-Min
GM
D031702:Agropyron / Q000235:genetics* / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis; D018506:Gene Expression Regulation, Plant; D006823:Hybrid Vigor; D006824:Hybridization, Genetic; D010940:Plant Proteins / Q000737:chemistry*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D012965:Sodium Chloride / Q000009:adverse effects; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*
D002352:Carrier Proteins; D010940:Plant Proteins; D020543:Proteome; D012965:Sodium Chloride