Bone morphogenetic protein-7 regulates differentially the mRNA expression of bone morphogenetic proteins and their receptors in rat achilles and patellar tendon cell cultures.
Previous animal studies have suggested that certain bone morphogenetic proteins (BMPs) may be useful therapeutically in treating tendon healing. To better understand the relationship among the different BMPs in the healing process, we initiated the present study to examine the effects of a member of the BMP family, BMP-7 (also called Osteogenic Protein-1) on the temporal and spatial expression patterns of other BMPs and the BMP receptors in cell cultures of adult rat Achilles and Patellar tendons. Cultures from both tendon types expressed detectable but variable levels of biochemical markers characteristics of tendons. RNAs coding for type II collagen and transcription factors Six1, Scleraxis, and Tendin were detected in both types of cultures. Distinct patterns of expression of several BMP members and their receptors were observed in these cultured cells and BMP-7 exerted differential effects on their expression. The findings may have implications in the treatment of different tendon injuries with BMPs.
Yeh
Lee-Chuan C
LC
Department of Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA. yehl@uthscsa.edu
Tsai
Alicia D
AD
Lee
John C
JC
D000125:Achilles Tendon / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015152:Blotting, Northern; D055419:Bone Morphogenetic Protein 7; D052004:Bone Morphogenetic Protein Receptors / Q000235:genetics* / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D023282:Nuclease Protection Assays; D010329:Patella / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D012260:Ribonucleases / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
C525819:BMP7 protein, human; D015415:Biomarkers; C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D052004:Bone Morphogenetic Protein Receptors; D012260:Ribonucleases