Cationized hydroxyethylcellulose as a novel, adsorbed coating for basic protein separation by capillary electrophoresis.
We present cationized hydroxyethylcellulose (cat-HEC) synthesized in our laboratory as a novel physically adsorbed coating for CE. This capillary coating is simple and easy to obtain as it only requires flushing the capillary with polymer aqueous solution. A comparative study with and without polymers was performed. The adsorbed cat-HEC coating exhibited minimal interactions with basic proteins, providing efficient basic protein separations with excellent reproducibility. Under broad pHs, the amine groups are the main charged groups bringing about a global positive charge on the capillary wall. As a consequence, the cat-HEC coating produced an anodal EOF performance. A comparative study on the use of hydroxyethylcellulose (HEC) and cat-HEC as physically adsorbed coatings for CE are also presented. The separation efficiency and analysis reproducibility proved that the cat-HEC polymer was efficient in suppressing the adsorption of basic proteins onto the silica capillary wall. The long-term stability of the cat-HEC coating in consecutive protein separation runs has demonstrated the suitability of the coating for high-throughput electrophoretic protein separations.
Yang
Runmiao
R
Joint Laboratory of Polymer Thin Films and Solution, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, PR China.
Shi
Ronghua
R
Peng
Shuhua
S
Zhou
Dan
D
Liu
Hang
H
Wang
Yanmei
Y
D000327:Adsorption; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins; D002482:Cellulose; C002283:hydroxyethylcellulose