title: Polycation nanostructured lipid carrier, a novel nonviral vector constructed with triolein for efficient gene delivery.
abstract: A novel nonviral gene transfer vector was developed by modifying nanostructured lipid carrier (NLC) with cetylated polyethylenimine (PEI). Polycation nanostructured lipid carrier (PNLC) was prepared using the emulsion-solvent evaporation method. Its in vitro gene transfer properties were evaluated in the human lung adenocarcinoma cell line SPC-A1 and Chinese Hamster Ovary (CHO) cells. Enhanced transfection efficiency of PNLC was observed after the addition of triolein to the PNLC formulation and the transfection efficiency of the optimized PNLC was comparable to that of Lipofectamine 2000. In the presence of 10% serum the transfection efficiency of the optimal PNLC was not significantly changed in either cell line, whereas that of Lipofectamine 2000 was greatly decreased in both. Thus, PNLC is an effective nonviral gene transfer vector and the gene delivery activity of PNLC was enhanced after triolein was included into the PNLC formulation.
authors.affiliation: School of Pharmacy, Fudan University, Lane 138, Yi Xue Yuan Road, Shanghai 200032, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xianyi
authors.initials: X
authors.lastname: Sha
authors.forename: Anle
authors.initials: A
authors.lastname: Shen
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhaogui
authors.initials: Z
authors.lastname: Sun
authors.forename: Zheng
authors.initials: Z
authors.lastname: Gu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Fang
mesh_terms: D000818:Animals; D016466:CHO Cells; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D005822:Genetic Vectors / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection; D014304:Triolein / Q000737:chemistry*
chemical_list: D002412:Cations; C485184:enhanced green fluorescent protein; D014304:Triolein; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine