title: Arachidonic acid-induced expression of the organic solute and steroid transporter-beta (Ost-beta) in a cartilaginous fish cell line.
abstract: The organic solute and steroid transporter (OST/Ost) is a unique membrane transport protein heterodimer composed of subunits designated alpha and beta, that transports conjugated steroids and prostaglandin E(2) across the plasma membrane. Ost was first identified in the liver of the cartilaginous fish Leucoraja erinacea, the little skate, and subsequently was found in many other species, including humans and rodents. The present study describes the isolation of a new cell line, LEE-1, derived from an early embryo of L. erinacea, and characterizes the expression of Ost in these cells. The mRNA size and amino acid sequence of Ost-beta in LEE-1 were identical to that previously reported for Ost-beta from skate liver, and the primary structure was identical to that of the spiny dogfish shark (Squalus acanthias) with the exception of a single amino acid. Ost-beta was found both on the plasma membrane and intracellularly in LEE-1 cells, consistent with its localization in other cell types. Interestingly, arachidonic acid, the precursor to eicosanoids, strongly induced Ost-beta expression in LEE-1 cells and a lipid mixture containing arachidonic acid also induced Ost-alpha. Overall, the present study describes the isolation of a novel marine cell line, and shows that this cell line expresses relatively high levels of Ost when cultured in the presence of arachidonic acid. Although the function of this transport protein in embryo-derived cells is unknown, it may play a role in the disposition of eicosanoids or steroid-derived molecules.
authors.affiliation: Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, USA.
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Hwang
authors.forename: Angela
authors.initials: A
authors.lastname: Parton
authors.forename: Anne
authors.initials: A
authors.lastname: Czechanski
authors.forename: Nazzareno
authors.initials: N
authors.lastname: Ballatori
authors.forename: David
authors.initials: D
authors.lastname: Barnes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D002460:Cell Line; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012860:Skates, Fish / Q000196:embryology / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D029941:Fish Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D013256:Steroids; D016718:Arachidonic Acid