title: [Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer].
abstract: OBJECTIVE
  To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer.
  
  
  METHODS
  A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test.
  
  
  RESULTS
  When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred.
  
  
  CONCLUSIONS
  Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.
authors.affiliation: Department of General Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Wang
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Zhou
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Dong-Bing
authors.initials: DB
authors.lastname: Zhao
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Ni
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Bai
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Gao
authors.forename: Yan-Tao
authors.initials: YT
authors.lastname: Tian
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
mesh_terms: D017305:Annexin A1 / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D017305:Annexin A1; D012333:RNA, Messenger; D034741:RNA, Small Interfering