Rubritalea sabuli sp. nov., a carotenoid- and squalene-producing member of the family Verrucomicrobiaceae, isolated from marine sediment.
The taxonomic status of a verrucomicrobial strain isolated from marine sediment was established based on a polyphasic examination. The novel isolate, strain YM29-052T, was obligately aerobic, Gram-negative, non-motile, coccoid or rod-shaped and chemoheterotrophic. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that the new isolate shared approximately 94-99 % sequence similarity with members of genus Rubritalea of the family Verrucomicrobiaceae within the phylum 'Verrucomicrobia'. Genomic DNA-DNA hybridization between strain YM29-052T and Rubritalea squalenifaciens HOact23T showed relatedness of <70 %, the value commonly accepted as the threshold for the phylogenetic definition of a species. Strain YM29-052T produces carotenoid compounds that render the cell biomass a pink colour; the strain also contains squalene. The cell-wall peptidoglycan of the novel strain contains muramic acid and meso-diaminopimelic acid. The DNA G+C content of strain YM29-052T was 47.7 mol%; MK-8 and MK-9 were the major menaquinones. The presence of iso-C14 : 0, iso-C16 : 0 and C16 : 1 omega 7c as major cellular fatty acids supported the identification of the novel isolate as a member of the genus Rubritalea. On the basis of polyphasic taxonomic evidence, it was concluded that strain YM29-052T should be classified within a novel species of the genus Rubritalea, for which the name Rubritalea sabuli sp. nov. is proposed. The type strain is YM29-052T (=MBIC08323T =KCTC 22127T).
Yoon
Jaewoo
J
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan. aa57058@mail.ecc.u-tokyo.ac.jp
Matsuo
Yoshihide
Y
Matsuda
Satoru
S
Adachi
Kyoko
K
Kasai
Hiroaki
H
Yokota
Akira
A
D001482:Base Composition; D002338:Carotenoids / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D019015:Geologic Sediments / Q000382:microbiology; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008849:Micronesia; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013185:Squalene / Q000378:metabolism; D009626:Terminology as Topic; D024482:Vitamin K 2 / Q000378:metabolism
D004269:DNA, Bacterial; D005227:Fatty Acids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D002338:Carotenoids; D013185:Squalene