An improved PCR method for gender identification of eagles.
Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay.
Chang
Hsueh-Wei
HW
Faculty of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan. changhw@kmu.edu.tw
Chou
Ta-Ching
TC
Gu
De-Leung
DL
Cheng
Chun-An
CA
Chang
Chia-Che
CC
Yao
Cheng-Te
CT
Chuang
Li-Yeh
LY
Wen
Cheng-Hao
CH
Chou
Yii-Cheng
YC
Tan
Kock-Yee
KY
Cheng
Chien-Chung
CC
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020307:Eagles / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis; D013045:Species Specificity
D017931:DNA Primers