Study of the interaction of beta-cyclodextrin with phospholipid monolayers by surface pressure measurements and fluorescence microscopy.
The interaction of beta-cyclodextrin (beta-CD) with different lipids has been studied, using Langmuir monolayers kept at constant surface pressure or constant spreading surface. Results show that beta-CD, injected beneath the monolayer, is able to desorb unsaturated palmitoyloleoylphosphatidylcholine (POPC) and sphingomyelin (SM) under specific experimental conditions. In this last case, SM monolayers, labeled with the fluorescent NBD-PC probe, were also observed by fluorescence microscopy, before and after beta-CD injection. Images show that SM monolayers are more homogeneous after beta-CD injection, because of the lipid desorption. At last, it seems that lipid desorption occurs only in a restricted surface pressure range, depending on the lipid.
Grauby-Heywang
Christine
C
Centre de Physique Moléculaire Optique et Hertzienne, UMR CNRS 5798 Université Bordeaux 1, 351 Cours de la Libération, 33405 Talence cedex, France. ch.heywang@cpmoh.u-bordeaux1.fr
Turlet
Jean-Marie
JM
D000327:Adsorption; D005456:Fluorescent Dyes / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000737:chemistry*
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010743:Phospholipids; D013501:Surface-Active Agents; D047392:beta-Cyclodextrins; C028694:1-palmitoyl-2-oleoylphosphatidylcholine