title: [The concentration of the pregnancy-associated plasma protein A (PAPP-A) in pregnant women smoking tobacco--preliminary study].
abstract: An improvement in prenatal screening for chromosomal defects in the first trimester of pregnancy has been achieved by combining sonography and biochemical markers. Among these markers exist the metalloproteinase pregnancy-associated plasma protein-A (PAPP-A) which promotes cell growth by proteolytic cleavage of insulin-like growth factor binding proteins (IGFBPs) causing the release of IGFs. The reduced level of PAPP-A in smokers in first-trimester has been reported previously. However, smoking influence on maternal level of PAPP-A during pregnancy has not been completely determined. The aim of this preliminary study was the investigation of PAPP-A concentrations in smoking women in the first- and third-trimester of pregnancy. The study comprised 40 pregnant women: 20 nonsmokers and 20 cigarette smokers. Only normal singleton pregnancies were included. Maternal serum levels of PAPP-A were measured with the KRYPTOR rapid random-access immunoassay analyser (Brahms GmbH, Germany) using time-resolved amplified cryptate emission technology (TRACE). In first-trimester of pregnancy we observed the lower of PAPP-A median values in smokers (2.77 IU/L, range 1.12-9.28 IU/L) by 24% than in nonsmokers (3.64 IU/L, range 1.68-6.79 IU/L). Next, we indicated that in third-trimester of pregnancy the PAPP-A median values were lower in smokers (48.45 IU/L, range 15.18-176.2 IU/L) until by 50% in comparison to the controls (96.56 IU/L, range 25.59-164.0 IU/L). Further studies will be continued to assessment the influence of some components from tobacco smoke on maternal serum PAPP-A in all trimesters of pregnancy.
authors.affiliation: Zaktad Badań Przesiewowych, Pracownia Biochemii Instytutu Matki i Dziecka w Warszawie. biochem@imid.med.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Gajewska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chełchowska
authors.forename: Alicja
authors.initials: A
authors.lastname: Ceran
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Ambroszkiewicz
authors.forename: Teresa
authors.initials: T
authors.lastname: Laskowska-Klita
authors.forename: Maciej
authors.initials: M
authors.lastname: Szymański
authors.forename: Jan
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000097:blood; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis*; D012907:Smoking / Q000009:adverse effects* / Q000097:blood*
chemical_list: D011266:Pregnancy-Associated Plasma Protein-A