title: BYHWD rescues axotomized neurons and promotes functional recovery after spinal cord injury in rats.
abstract: BACKGROUND
  Buyang Huanwu Decoction (BYHWD), a Chinese prescription that has been used for hundreds of years to treat paralysis, has gained attention recently due to its significant neuroprotective properties.
  
  
  OBJECTIVE
  This study was to investigate whether BYHWD treatment protected axotomized rubrospinal neurons (RN) following spinal cord injury (SCI) in rats.
  
  
  METHODS
  Adult rats received a right lateral funiculus transection at the level between C3 and C4, and were either treated with BYHWD or with distilled water (DW) via gastrogavage. Effects on RN viability and atrophy were determined by Nissl staining, axon regeneration was examined by biotinylated dextran amine (BDA) tracing techniques and functional recovery was studied by a test of forelimb usage during spontaneous vertical exploration.
  
  
  RESULTS
  RN cell number and mean somal size were 20% and 29% higher, respectively, in BYHWD-treated rats relative to DW-treated rats. There were a small number of BDA-labeled axons in the caudal of injury site in BYHWD-treated rats, whereas no caudal axonal regeneration was detected in DW-treated rats. BYHWD-treated rats used the injured forelimb more often than rats treated with DW.
  
  
  CONCLUSIONS
  These results indicate that administration of BYHWD following SCI protects injured neurons, promotes regeneration, and enhances functional recovery.
authors.affiliation: Department of Anatomy & Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, China.
authors.forename: An
authors.initials: A
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Liao
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Weijun
authors.initials: W
authors.lastname: Cai
authors.forename: Xuegang
authors.initials: X
authors.lastname: Luo
authors.forename: Gong
authors.initials: G
authors.lastname: Ju
mesh_terms: D000818:Animals; D001284:Atrophy; D019771:Axotomy; D001522:Behavior, Animal / Q000187:drug effects; D001710:Biotin / Q000031:analogs & derivatives; D002452:Cell Count; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D005456:Fluorescent Dyes; D009416:Nerve Regeneration / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012012:Red Nucleus / Q000166:cytology / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000473:pathology
chemical_list: D003911:Dextrans; D004365:Drugs, Chinese Herbal; D005456:Fluorescent Dyes; C076397:biotinylated dextran amine; C460102:buyang huanwu; D001710:Biotin