Simple preparation of baicalin from Scutellariae Radix.
A simple, rapid and reproducible method was developed to isolate high-purity baicalin from Scutellariae Radix, the dried roots of Scutellaria baicalensis Georgi. The method involves partition/recrystallization steps without repeated column chromatography or special instruments. Isolated baicalin was characterized by comparisons of TLC, HPLC, IR, MS, and NMR data with an authentic sample. Moreover, beta-glucuronidase hydrolysis of baicalin sequentially yielded glucuronic acid and baicalein as confirmed by co-TLC with authentic samples. The purity of baicalin was more than 97% with yield ca. 8.7% (w/w). The method presented here appears suitable for commercial application.
Ohkoshi
Emika
E
The School of Pharmaceutical Sciences, Ohu University 31-1, Misumidou Tomita-Machi, Kooriyama-shi, Fukushima 963-8611, Japan. e-ohkoshi@pha.ohu-u.ac.jp
Nagashima
Tomomi
T
Sato
Hiroyasu
H
Fujii
Yuichi
Y
Nozawa
Koohei
K
Nagai
Masahiro
M
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin