Polymorphism of the methylenetetrahydrofolate reductase C677T gene with chronic allograft nephropathy in renal transplant recipients.
OBJECTIVE
This study sought to investigate the frequency of the 5, 10-methylenetetrahydrofolate reductase gene (MTHFR C677T) in 127 patients (77 with chronic allograft nephropathy and 50 with normal renal function) who had undergone a renal transplant at least 20 months earlier to define the risk factors for chronic allograft dysfunction. Fifty healthy subjects served as controls.


METHODS
Genotypes were determined using polymerase chain reaction followed by restriction fragment length polymorphism analysis. The restriction enzyme for the MTHFR C677T variants was HinfI.


RESULTS
No statistically significant differences were seen between the allelic and genotypic distribution of the MTHFR polymorphism.


CONCLUSIONS
Additional studies with larger sample sizes are needed to define the influence of MTHFR C677T genotyping on clinical outcomes in renal allograft recipients.
Azarpira
Negar
N
Organ Transplant Research Center, Nephrology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. negarazarpira@yahoo.com
Raisjalali
Ghanbar
G
Darai
Masumeh
M
D000328:Adult; D002908:Chronic Disease; D005260:Female; D005838:Genotype; D006801:Humans; D007674:Kidney Diseases / Q000209:etiology*; D016030:Kidney Transplantation; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011183:Postoperative Complications; D012307:Risk Factors; D014184:Transplantation, Homologous
D042965:Methylenetetrahydrofolate Reductase (NADPH2)