title: The viral bronchiolites diagnosis in children by PCR multiplex.
abstract: The aim of the study was to determine the etiology of the viral bronchiolites in children by using direct immunofluorescence test and 3 RT-PCR Multiplex (S.Bellau-Pujol) The study was performed on 122 nasal inspirations collected from 3 weeks-6 month old children hospitalizated in the pediatrics service of CH Rouen. The results were that the majority (53%) of bronchiolites in children had like etiology RSV and a lot of these infections had double viral etiology (26% RSV+ Rhinovirus; 2,7% RSV+HMPV and 1% RSV+Coronavirus 229E). An important viral factor which gives bronchiolitis in children is HMPV (11%). We also find respiratory infections with triple viral etiology: RSV+Influenza A virus + Rhinovirus.
authors.affiliation: NIRDMI Cantacuzino, Bucharest, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Tecu
authors.forename: Evelyne
authors.initials: E
authors.lastname: Genetay
authors.forename: Viorel
authors.initials: V
authors.lastname: Alexandrescu
authors.forename: Astrid
authors.initials: A
authors.lastname: Vabret
authors.forename: F
authors.initials: F
authors.lastname: Freymuth
mesh_terms: D001988:Bronchiolitis / Q000175:diagnosis*; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000235:genetics*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*