High adiponectin and adiponectin receptor 1 expression in synovial fluids and synovial tissues of patients with rheumatoid arthritis.
OBJECTIVE
The major objectives of this article are first to measure the levels of expression of adiponectin and its 2 receptors (adipoR1 and adipoR2) in the peripheral blood mononuclear cells and in the synovial compartment of rheumatoid arthritis (RA) patients, and second, to assess their pro-inflammatory potential. Osteoarthritis patients and healthy subjects served as controls.


METHODS
Expression of adiponectin, adipoR1, and adipoR2 were assayed by real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistology. The potential pro-inflammatory activity of adiponectin was studied by adding recombinant adiponectin to cultures of synovial fibroblasts.


RESULTS
Immunohistology showed that numerous cells in the synovial biopsies of RA expressed adiponectin, adipoR1, and adipoR2. The synovial fibroblasts were distinctly rich in adiponectin. As expected, high adiponectin levels were present in the synovial fluids. In contrast to the synovial compartment, in peripheral blood mononuclear cells, only adipoR1 exceeded those of osteoarthritis and healthy subjects. When recombinant adiponectin was added to cultures of synovial fibroblasts, it induced up to 8.1- and 11.4-fold increase in the release of monocyte chemoattractant protein-1 and interleukin-6.


CONCLUSIONS
The adiponectin adipokine axis might play a role in RA.
Tan
Wenfeng
W
Department of Rheumatology, Clinical Experiment Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Wang
Fang
F
Zhang
Miaojia
M
Guo
Dunming
D
Zhang
Qiande
Q
He
Shaoheng
S
D052242:Adiponectin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000378:metabolism* / Q000503:physiopathology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000378:metabolism; D007596:Joints / Q000503:physiopathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
D052242:Adiponectin; D018932:Chemokine CCL2; D015850:Interleukin-6; D012333:RNA, Messenger; D054419:Receptors, Adiponectin; D011994:Recombinant Proteins