title: Association between oral lichenoid reactions and amalgam restorations.
abstract: BACKGROUND
  The aim of this study was to perform a clinical assessment of the association between oral lichenoid reactions (OLR) and amalgam restorations and to determine the salivary concentrations of interleukin-6 (IL-6) and IL-8 before and after replacement of the amalgam restorations.
  
  
  METHODS
  The study included 20 patients with OLR and 20 healthy volunteers, who were examined between 2001 and 2005 at the Oral Medicine Unit of the Medical Faculty University of Rijeka. All patients were skin patch tested by an experienced physician. Saliva samples were collected, prepared and analysed for IL-6 and IL-8 concentrations using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Sixteen out of 20 patch-tested patients showed a sensitization to inorganic mercury or amalgam. Total replacement of all amalgam fillings was carried out on 20 patients with fillings based on composite resin, gold, porcelain or a combination of these. Sixteen out of 20 patients showed complete healing of OLR; three patients had marked improvement, whereas one patient showed no improvement. Levels of IL-6 detected before replacement were significantly higher than IL-6 levels following the replacement (P = 0.003). The IL-8 levels measured before replacement procedure were significantly higher than the IL-8 levels after replacement of the fillings (P < 0.001).
  
  
  CONCLUSIONS
  On the basis of clinical observations, restorative therapy resulted in tissue healing. Following the replacement of amalgam fillings with fillings based on other restorative materials, levels of both IL-6 and IL-8 shifted towards normal, as measured in healthy subjects.
authors.affiliation: Medical Faculty, University of Rijeka, Department of Oral pathology, Rijeka, Croatia. sonja.pezelj-ribaric@medri.hr
authors.forename: S
authors.initials: S
authors.lastname: Pezelj-Ribarić
authors.forename: J
authors.initials: J
authors.lastname: Prpić
authors.forename: I
authors.initials: I
authors.lastname: Miletić
authors.forename: G
authors.initials: G
authors.lastname: Brumini
authors.forename: M Simunović
authors.initials: MS
authors.lastname: Soskić
authors.forename: I
authors.initials: I
authors.lastname: Anić
mesh_terms: D016022:Case-Control Studies; D003723:Dental Amalgam / Q000009:adverse effects*; D003793:Dental Restoration, Permanent; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D017512:Lichenoid Eruptions / Q000209:etiology* / Q000473:pathology; D009059:Mouth Diseases / Q000209:etiology* / Q000473:pathology; D012463:Saliva / Q000378:metabolism; D012882:Skin Tests
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D003723:Dental Amalgam