Staining efficiency of specific proteins depends on the staining method: wheat gluten proteins.
To analyze gluten proteins involved in celiac disease (CD) by proteomic analysis, prolamins extracted from hexaploid wheat varieties were analyzed by SDS-PAGE and 2-DE. Differences between staining methods (CBB, silver nitrate, SYPRO Ruby, and CyDye) were analyzed in comparison to immunoblotting. Staining efficiency varied per protein across methods, and complete staining of all gluten proteins could not be achieved by one of these methods. Care should be taken in the selection of staining method especially if one wants to relate the results to data obtained by immunoblotting.
van den Broeck
Hetty C
HC
Plant Research International, Wageningen-UR, Wageningen, The Netherlands.
America
Antoine H P
AH
Smulders
Marinus J M
MJ
Gilissen
Ludovicus J W J
LJ
van der Meer
Ingrid M
IM
D002446:Celiac Disease / Q000378:metabolism; D004396:Coloring Agents / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005983:Glutens / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D009942:Organometallic Compounds / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry; D055315:Prolamins; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining / Q000379:methods; D012995:Solubility; D013194:Staining and Labeling / Q000295:instrumentation* / Q000379:methods; D014908:Triticum / Q000378:metabolism*
D004396:Coloring Agents; D009942:Organometallic Compounds; D010940:Plant Proteins; D055315:Prolamins; D020543:Proteome; C515601:Sypro Ruby; D005983:Glutens