Chorionic villus sampling for early prenatal diagnosis at Bhumibol Adulyadej Hospital.
OBJECTIVE
To evaluate results of chorionic villus sampling for early prenatal diagnosis at Bhumibol Adulyadej Hospital.


METHODS
Retrospective descriptive study.


METHODS
Perinatal unit, Department of Obstetrics and Gynaecology, Bhumibol Adulyadej Hospital.


METHODS
Three hundred and eighty three women were enrolled to chorionic villus sampling at the perinatal unit, Department of Obstetrics and Gynecology, Bhumibol Adulyadej Hospital, from November 10,1997 to October 17, 2006.


RESULTS
During the present study periods three hundred and eighty three women were recruited, of these chorionic villus sampling for chromosome diagnosis were performed on 355 while 6 were for abnormal Thalassemia screening. Twenty two cases were excluded because ultrasound examination showed anembryonic pregnancy or fetal demise in utero in 13 cases, multiple fibroids in 4 cases, large area of placental hemorrhage in 3 cases, 1 case of multiple pregnancy and in 1 case the placenta was in an inappropriate position. The most common indication was elderly gravidarum (95.84%). Other indications were abnormal Thalassemia screening, abnormal ultrasound findings, family chromosome disorder previous Down syndrome, and severe oligohydramnios. The authors found eleven cases of chromosome abnormalities, four cases of maternal cell contamination and three cases of failed tissue culture (two cases from transcervical chorionic villus sampling and one case from transabdominal chorionic villus sampling) and two cases of mosaicism. There were two fetal losses in the present study and all the babies from the normal chromosome result looked normal. Second trimester amniocentesis following chorionic villus sampling was required due to maternal cell contamination, mosaicism and failed tissue culture. (2.77%) All cases had follow-up ultrasound scan during 18-20 weeks.


CONCLUSIONS
The authors found that chorionic villus sampling is a possible alternative technique for prenatal diagnosis of cytogenetic abnormalities and abnormal Thalassemia screening in Thailand. It probably has a slightly higher rate of failed tissue culture and maternal cell contamination than amniocentesis, but it is generally done earlier in pregnancy than amniocentesis and is particularly advantageous for detecting certain genetic conditions.
Rueangchainikhom
Wibool
W
Department of Obstetrics and Gynaecology, Bhumibol Adulyadej Hospital, Bangkok, Thailand.
Sarapak
Saravut
S
Orungrote
Nawaporn
N
D000328:Adult; D015193:Chorionic Villi Sampling / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006779:Hospitals, Public; D006801:Humans; D011247:Pregnancy; D011295:Prenatal Care; D011296:Prenatal Diagnosis; D012189:Retrospective Studies; D013785:Thailand; D013789:Thalassemia / Q000175:diagnosis; D013997:Time Factors