Multispectral fluorescence lifetime imaging of feces-contaminated apples by time-resolved laser-induced fluorescence imaging system with tunable excitation wavelengths.
We recently developed a time-resolved multispectral laser-induced fluorescence (LIF) imaging system capable of tunable wavelengths in the visible region for sample excitation and nanosecond-scale characterizations of fluorescence responses (lifetime imaging). Time-dependent fluorescence decay characteristics and fluorescence lifetime imaging of apples artificially contaminated with a range of diluted cow feces were investigated at 670 and 685 nm emission bands obtained by 418, 530, and 630 nm excitations. The results demonstrated that a 670 nm emission with a 418 nm excitation provided the greatest difference in time-dependent fluorescence responses between the apples and feces-treated spots. The versatilities of the time-resolved LIF imaging system, including fluorescence lifetime imaging of a relatively large biological object in a multispectral excitation-emission wavelength domain, were demonstrated.
Kim
Moon S
MS
Food Safety Laboratory, Animal and Natural Resources Institute, Henry A. Wallace Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705, USA. moon.kim@ars.usda.gov
Cho
Byoung-Kwan
BK
Lefcourt
Alan M
AM
Chen
Yud-Ren
YR
Kang
Sukwon
S
D005243:Feces / Q000737:chemistry*; D005453:Fluorescence; D007834:Lasers; D027845:Malus / Q000737:chemistry*