Isolation and characterization of Acidithiobacillus ferrooxidans strain D3-2 active in copper bioleaching from a copper mine in Chile.
Acidithiobacillus ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide ore dump in Chile. The amounts of Cu(2+) solubilized from 1% chalcopyrite (CuFeS(2)) concentrate medium (pH 2.5) by A. ferrooxidans strains D3-2, D3-6, and ATCC 23270 and 33020 were 1360, 1080, 650, and 600 mg x l(-1) x 30 d(-1). The iron oxidase activities of D3-2, D3-6, and ATCC 23270 were 11.7, 13.2, and 27.9 microl O(2) uptake x mg protein(-1) x min(-1). In contrast, the sulfite oxidase activities of strains D3-2, D3-6, and ATCC 23270 were 5.8, 2.9, and 1.0 mul O(2) uptake.mg protein(-1).min(-1). Both of cell growth and Cu-bioleaching activity of strains D3-6 and ATCC 23270, but not, of D3-2, in the chalcopyrite concentrate medium were completely inhibited in the presence of 5 mM sodium bisulfite. The sulfite oxidase of strain D3-2 was much more resistant to sulfite ion than that of strain ATCC 23270. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm that strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase.
Sugio
Tsuyoshi
T
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-naka, Okayama 700-8530, Japan. sugio@cc.okayama-u.ac.jp
Wakabayashi
Masanori
M
Kanao
Tadayoshi
T
Takeuchi
Fumiaki
F
D042763:Acidithiobacillus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D002677:Chile; D003300:Copper / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D008906:Mining; D010084:Oxidation-Reduction; D012995:Solubility / Q000187:drug effects; D013447:Sulfites / Q000494:pharmacology
D003470:Culture Media; D013447:Sulfites; C012819:chalcopyrite; D003300:Copper; D007501:Iron; C009279:sodium bisulfite