title: Electrical characterization of protein molecules by a solid-state nanopore.
abstract: The authors measured ionic current blockages caused by protein translocation through voltage-biased silicon nitride nanopores in ionic solution. By calculating the mean amplitude, time duration, and the integral of current blockages, they estimated the relative charge and size of protein molecules at a single molecule level. The authors measured the change in protein charge of bovine serum albumin (BSA) protein induced by pH variation. They also confirmed that BSA molecules indeed traverse nanopores using an improved chemiluminescent analysis. They demonstrated that a larger protein fibrinogen could be distinguished from BSA by a solid-state nanopore measurement.
authors.affiliation: Department of Physics, University of Arkansas, Fayetteville, Arkansas 72701.
authors.forename: Daniel
authors.initials: D
authors.lastname: Fologea
authors.forename: Bradley
authors.initials: B
authors.lastname: Ledden
authors.forename: David S
authors.initials: DS
authors.lastname: McNabb
authors.forename: Jiali
authors.initials: J
authors.lastname: Li