title: Integrated Raman- and angular-scattering microscopy.
abstract: A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10 mum wide. The elastic-scattering signal was analyzed by generalized Lorenz-Mie theory, representing what we believe to be the first experimental validation of the theory's prediction of angular backscatter from single spheres. The microscope system exhibits 3 nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned.
authors.affiliation: The Institute of Optics, University of Rochester, Rochester, NY 14627, USA.
authors.forename: Zachary J
authors.initials: ZJ
authors.lastname: Smith
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Berger
mesh_terms: D004548:Elasticity; D004867:Equipment Design; D007834:Lasers; D008027:Light; D008853:Microscopy; D008962:Models, Theoretical; D055095:Optics and Photonics; D009991:Oscillometry; D011137:Polystyrenes / Q000737:chemistry; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D011137:Polystyrenes