title: Effect of temperature on Brettanomyces bruxellensis: metabolic and kinetic aspects.
abstract: The effect of temperatures ranging from 15 to 35 degrees C on a culture of Brettanomyces bruxellensis was investigated in regards to thermodynamics, metabolism, and kinetics. In this temperature range, we observed an increase in growth and production rates. The growth behavior was well represented using the Arrhenius model, and an apparent activation energy of 16.61 kcal/mol was estimated. A stuck fermentation was observed at 35 degrees C as represented by high cell death. The carbon balance established that temperature had no effect on repartition of the glucose consumption between biomass and products. Hence, the same biomass concentration was obtained for all temperatures, except at 35 degrees C. Moreover, using logistic and Luedeking-Piret models, we demonstrated that production rates of ethanol and acetic acid were partially growth associated. Parameters associated with growth (alpha eth and alpha aa) remained constant with changing temperature, whereas, parameters associated with the population (beta eth and beta aa) varied. Optimal values were obtained at 32 degrees C for ethanol and at 25 degrees C for acetic acid.
authors.affiliation: Laboratoire de genie chimique, UMR-CNRS 5503, 5 rue Paulin Talabot, 31106 Toulouse CEDEX 1, France. Cedric.Brandam@ensiacet.fr
authors.forename: Cédric
authors.initials: C
authors.lastname: Brandam
authors.forename: Claudia
authors.initials: C
authors.lastname: Castro-Martínez
authors.forename: Marie-Line
authors.initials: ML
authors.lastname: Délia
authors.forename: Felipe
authors.initials: F
authors.lastname: Ramón-Portugal
authors.forename: Pierre
authors.initials: P
authors.lastname: Strehaiano
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D016015:Logistic Models; D008954:Models, Biological; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
chemical_list: D000431:Ethanol; D019342:Acetic Acid