title: [Effect of aurora A on carcinogenesis of human prostate cancer].
abstract: OBJECTIVE
  To investigate whether Aurora A is involved in prostate cancer carcinogenesis.
  
  
  METHODS
  The expressions of Aurora A mRNA and protein in prostate cancer tissue and cell line were measured using RT-PCR, immunohistochemistry and Western blot analyses. Meanwhile, A DNAzyme targeting at Aurora A mRNA was performed to detect the inhibition effect of Aurora A in regulating the growth of prostate cancer cell line PC3.
  
  
  RESULTS
  Aurora A expression was up-regulated in 91% prostate cancer tissue as well as in prostate cancer cell lines such as PC3, LNCaP and Du145. Furthermore, Aurora A expression suppressed by cell transduction of a DNAzyme targeting at Aurora A mRNA made the PC3 cells both arrest the cell cycle in G2/M phase and increase the cell apoptosis.
  
  
  CONCLUSIONS
  These findings suggest that Aurora A up-regulation may be involved in carcinogenesis of prostate cancer, and that aurora A gene may be a valuable target for treatment of prostate cancer.
authors.affiliation: Experimental Center, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Qu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Mu
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Wei
authors.forename: Feng-yan
authors.initials: FY
authors.lastname: Zhao
authors.forename: Bai-lin
authors.initials: BL
authors.lastname: Liu
mesh_terms: D017209:Apoptosis; D064127:Aurora Kinases; D002453:Cell Cycle; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases