title: AtCXXS: atypical members of the Arabidopsis thaliana thioredoxin h family with a remarkably high disulfide isomerase activity.
abstract: The Arabidopsis thaliana thioredoxin subgroup h III is composed of four members and includes the two monocysteinic (CXXS) thioredoxins encoded by the genome. We show that AtCXXS1 is the ortholog of monocysteinic thioredoxins present in all higher plants. In contrast, unicellular algae and the moss Physcomitrella patens do not encode monocysteinic thioredoxin. AtCXXS2, the second monocysteinic thioredoxin of Arabidopsis has no ortholog in any other higher plants. It probably appeared recently by duplications of a dicysteinic thioredoxin of the same subgroup h III. Both monocysteinic thioredoxins show a low disulfide reductase activity in vitro but are very efficient as disulfide isomerases in RNAse refolding tests. The possible interactions of these proteins with the glutathione glutaredoxin pathway are discussed on the basis of recent papers.
authors.affiliation: Laboratoire Génome et Développement des Plantes, UMR CNRS-IRD-UPVD 5096, Université de Perpignan, Perpignan, Cedex, France.
authors.forename: Antonio Jesús
authors.initials: AJ
authors.lastname: Serrato
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Guilleminot
authors.forename: Yves
authors.initials: Y
authors.lastname: Meyer
authors.forename: Florence
authors.initials: F
authors.lastname: Vignols
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D003600:Cytosol / Q000378:metabolism; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D054480:Thioredoxin h / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D054480:Thioredoxin h; D049452:Green Fluorescent Proteins; D019704:Protein Disulfide-Isomerases