Anticarcinogenic compounds in the Uzbek medicinal plant, Helichrysum maracandicum.
An ethanol extract of Helichrysum maracandicum showed antiproliferative activity against cultured cells of SENCAR mouse in an in vitro assay, and activity-guided fractionation of the extract resulted in the isolation of isosalipurposide as an active substance. Naringenin chalcone, the aglycone of isosalipurposide, also showed strong antiproliferative activity. An in vivo assay of two-stage carcinogenesis on mouse skin revealed that epidermal application of isosalipurposide resulted in delayed formation of papillomas. Western blot analysis showed that the expression of p38 mitogen-activated protein kinase was suppressed by the administration of naringenin chalcone or isosalipurposide, which might be related to the anticarcinogenic activity.
Yagura
Toru
T
Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimoadachi, Sakyo-ku, Kyoto, 606-8501, Japan.
Motomiya
Tomoko
T
Ito
Michiho
M
Honda
Gisho
G
Iida
Akira
A
Kiuchi
Fumiyuki
F
Tokuda
Harukuni
H
Nishino
Hoyoku
H
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000302:isolation & purification*; D015153:Blotting, Western; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification*; D003043:Cocarcinogenesis; D005260:Female; D035264:Flowers / Q000737:chemistry; D036562:Helichrysum / Q000737:chemistry*; D051379:Mice; D018632:Mice, Inbred SENCAR; D010212:Papilloma / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control; D010703:Phorbol Esters; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal; D012878:Skin Neoplasms / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control; D014610:Uzbekistan; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
D016588:Anticarcinogenic Agents; D047188:Chalcones; D010703:Phorbol Esters; D010936:Plant Extracts; C027321:isosalipurposide; D015127:9,10-Dimethyl-1,2-benzanthracene; C027329:naringenin chalcone; D048051:p38 Mitogen-Activated Protein Kinases