[Determination of bisphenol A and alkyl phenols in canned food with high performance liquid chromatography--fluorescence].
OBJECTIVE
To establish a comprehensive analytical high performance liquid chromatography fluorescence detection (HPLC-FL) in detecting bisphenol A (BPA), nonylphenol (NP) and octylphenol (OP) in canned food sold in Beijing markets.


METHODS
BPA, NP and OP was extracted with methanol and dichloroacetamide and concentrated. The samples were purified on an solid extraction cartridges. The HPLC system consisted of Waters XTerra MS C18 column, a mixture of methanol and water as mobile phase and fluorescence detector with the excitation and emission wavelength at 225 nm and 310 nm respectively.


RESULTS
The method established had a linear relationship, showing the detection limit of BPA, OP and NP being 0.5, 0.1 and 0.1 microg/kg in canned vegetable and instant noodle and 1, 0.5 and 0.5 microg/kg in canned fish and meat can, respectively. The recoveries of BPA, NP and OP were 74.9%-95.1% , 76.3%-103.6% and 72.1%-109.2%. The precision was 4.98%-11.2% , 2.35%-8.88% and 5.61%-12.3%, respectively.


CONCLUSIONS
The method is simple with high sensitivity and selectivity, suitable for the determination of NP, OP and BPA in canned food.
Xiao
Jing
J
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China.
Shao
Bing
B
Wu
Yong-Ning
YN
Wang
Zhu-Tian
ZT
Hou
Wei
W
D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid; D005513:Food Inspection / Q000379:methods*; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D010636:Phenols; C474055:octylphenol; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds