title: In vitro positive biocompatibility evaluation of glass-glass ceramic thermoseeds for hyperthermic treatment of bone tumors.
abstract: A new kind of magnetic thermoseed for bone tissue engineering has been synthesized. The materials used are specially designed to restore bone tissue after tumor extirpation, because they exhibit bioactive behavior and the ability to act as thermoseeds for cancer treatment using hyperthermia. The L929 cell line of mouse fibroblasts has been used in a wide biocompatibility study concerning cell proliferation and morphology studies, mitochondrial function determination, lactate dehydrogenase measurement, and flow cytometry studies, including cell cycle analysis, cell size and complexity, and intracellular reactive oxygen species content. The results presented in this work indicate that these bioactive magnetic materials are highly biocompatible and show greater cell response for thermoseeds with a higher magnetic phase content. There were no significant alterations detected in the cell cycle, and the interaction between fibroblasts and the different mixtures did not induce significant apoptosis.
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
authors.forename: M Concepción
authors.initials: MC
authors.lastname: Serrano
authors.forename: M  Teresa
authors.initials: M
authors.lastname: Portolés
authors.forename: Raffaella
authors.initials: R
authors.lastname: Pagani
authors.forename: Juan Sáez
authors.initials: JS
authors.lastname: de Guinoa
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ruiz-Hernández
authors.forename: Daniel
authors.initials: D
authors.lastname: Arcos
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials; D001859:Bone Neoplasms / Q000628:therapy*; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D002516:Ceramics; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005898:Glass; D006979:Hyperthermia, Induced / Q000379:methods*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; C023506:Glass ceramics; D007770:L-Lactate Dehydrogenase