title: Numerical analysis of thermal lens effect for sensitive detection on microchips.
abstract: Thermal lens microscope (TLM) is a sensitive detection method for nonfluorescent molecules and widely applied to detection in a capillary or on a microchip. In this paper, we developed a flexible design tool for TLM systems to meet various applications utilizing a microspace. The TL effect was modeled, including signal processing, and calculated by combining fluidic dynamics and wave optics software. The coincidence of the calculations and experiments was investigated by measuring the effects of optical path length or focus positions of the excitation beams on TL signals which are quite difficult to calculate by a conventional method. Good agreement was shown and the applicability of the TLM design tool was verified.
authors.affiliation: Technical Research Laboratory, Nippon Sheet Glass Co. Ltd., Osaka, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Anraku
authors.forename: Kazuma
authors.initials: K
authors.lastname: Mawatari
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Nara
authors.forename: Takahiro
authors.initials: T
authors.lastname: Asai
authors.forename: Akihiko
authors.initials: A
authors.lastname: Hattori
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kitamori
mesh_terms: D007909:Lenses; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D008962:Models, Theoretical; D055095:Optics and Photonics; D012984:Software