title: [Ectopic expression of keratin 19 and integrin beta1 during wound healing process after microskin grafting in rats].
abstract: OBJECTIVE
  To observe the characteristics of keratin 19 and integrin beta1 expressions in the wound after microskin grafting , and to investigate the healing mechanism.
  
  
  METHODS
  Full layer skin defects were created in twenty Sprague-Dawley rats and they were divided into two groups, i.e., A group (with grafting of autologous microskin accounting 10% in weight of epidermis loss from skin defect), B group (with grafting of autologous microskin and allogeneic microskin, accounting 10% and 40% weight of epidermis loss respectively in skin defect). The wound healing rate and contraction rate were observed at 2,3,4 post-grafting week (PGW), and the expression and distribution of keratin 19 and integrin beta1 were observed at 2 and 4 PGW.
  
  
  RESULTS
  The wound healing rate in the B group on 2 and 3 PGW was obviously higher than that in A group [(85 +/- 5)% vs. (53 +/- 10)%, (84 +/- 8)% vs. (65 +/- 9)%, P < 0.01]. No obvious difference in wound contraction rate between the two groups was observed on the 2, 3 and 4 PGW (P > 0.05). Cells with expression of keratin 19 and integrin beta1 were observed in the suprabasal layers of the epidermis in healing wound, but not in the basal membrane. Integrin beta1 positive expression cells were not observed in the suprabasal layers until 4 PGW.
  
  
  CONCLUSIONS
  Mixed grafting with autogenous and allogenous microskin can improve wound healing. Ectopic expression of keratin 19 and integrin beta1 exists during wound healing process after microskin grafting.
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, the Third Military Medical University, Chongqing, PR China.
authors.forename: Yan-ni
authors.initials: YN
authors.lastname: Yang
authors.forename: Dai-zhi
authors.initials: DZ
authors.lastname: Peng
authors.forename: Su-hua
authors.initials: SH
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Zheng-xue
authors.initials: ZX
authors.lastname: Dong
mesh_terms: D000818:Animals; D005260:Female; D019012:Integrin beta1 / Q000378:metabolism*; D053539:Keratin-19 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism*; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D014945:Wound Healing
chemical_list: D019012:Integrin beta1; D053539:Keratin-19