title: The vacuolar V1/V0-ATPase is involved in the release of the HOPS subunit Vps41 from vacuoles, vacuole fragmentation and fusion.
abstract: At yeast vacuoles, phosphorylation of the HOPS subunit Vps41 depends on the Yck3 kinase. In a screen for mutants that mimic the yck3Delta phenotype, in which Vps41 accumulates in vacuolar dots, we observed that mutants in the V0-part of the V0/V1-ATPase, in particular in vma16Delta, also accumulate Vps41. This accumulation is not due to a phosphorylation defect, but to reduced release of Vps41 from vma16Delta vacuoles. One reason could be a connection to vacuole fission, which is blocked in V-ATPase mutants. Vacuole fusion is not impaired between vacuoles lacking the V0-subunits Vma16 or Vma6 and wild-type vacuoles, whereas fusion between mutant vacuoles is reduced. Our data suggest a connection between vacuole biogenesis and membrane fusion.
authors.affiliation: Department of Biology, University of Osnabrück, Barbarastrasse 13, 49076 Osnabrück, Germany.
authors.forename: Kozue
authors.initials: K
authors.lastname: Takeda
authors.forename: Margarita
authors.initials: M
authors.lastname: Cabrera
authors.forename: Jan
authors.initials: J
authors.lastname: Rohde
authors.forename: Dirk
authors.initials: D
authors.lastname: Bausch
authors.forename: Ole N
authors.initials: ON
authors.lastname: Jensen
authors.forename: Christian
authors.initials: C
authors.lastname: Ungermann
mesh_terms: D047389:Casein Kinase I / Q000235:genetics; D008561:Membrane Fusion; D009154:Mutation; D010766:Phosphorylation; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology*; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C083878:VPS41 protein, S cerevisiae; D033921:Vesicular Transport Proteins; D047389:Casein Kinase I; C479667:YCK3 protein, S cerevisiae; D025262:Vacuolar Proton-Translocating ATPases