title: Frequencies of PRNP gene polymorphisms in Vietnamese dairy cattle for potential association with BSE.
abstract: Summary Since 2004, significant associations between bovine spongiform encephalopathy (BSE) susceptibility in cattle and frequencies of insertion/deletion (ins/del; indel) polymorphisms within the bovine prion protein gene (PRNP) have been reported. In this study, we investigated the frequencies of indel polymorphisms within two variable sites, a 23-bp indel polymorphism in the promoter region (23indel) and a 12-bp indel polymorphism in intron 1 region (12indel), in the PRNP in 206 Vietnamese dairy cattle and seven Japanese BSE-affected cattle. In Vietnamese dairy cattle, the frequency distributions of del allele and del/del genotypic polymorphisms in the 23indel site, which are thought to be associated with BSE susceptibility, were significantly higher, whereas the frequencies of del allelic and del/del genotypic polymorphisms in the 12indel site, which have been reported to confer BSE susceptibility, were significantly lower. We have provided evidence that Vietnamese dairy cattle have a unique genetic background in the PRNP gene in comparison with cattle or sires previously reported in other countries.
authors.affiliation: Department of Veterinary Public Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan. y-mrmt@rakuno.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Muramatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Sakemi
authors.forename: M
authors.initials: M
authors.lastname: Horiuchi
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Kanameda
authors.forename: T T
authors.initials: TT
authors.lastname: Hanh
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
mesh_terms: D000818:Animals; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D011110:Polymorphism, Genetic; D011328:Prions / Q000235:genetics*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014744:Vietnam
chemical_list: D011328:Prions