Interaction of Rab3B with microtubule-binding protein Gas8 in NIH 3T3 cells.
Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3B is also detected and upregulated in non-neuronal/non-secretory cells, whereas its function remains elusive. In the present study, we identified growth-arrest-specific gene 8 (Gas8), an evolutionally conserved microtubule-binding protein that is upregulated in growth-arrested NIH 3T3 cells and involved in the dynein motor regulation in flagellar/ciliary axoneme, as a novel Rab3B-binding protein using a yeast two-hybrid system. Rab3B as well as Gas8 was upregulated in growth-arrested NIH 3T3 cells and enriched in testis and lung with well-developed flagella/cilia. Gas8 was specifically interacted with the GTP-bound form of Rab3B and co-localized with Rab3B at the Golgi in NIH 3T3 cells. Furthermore, Rab3B was relocated upon expression of the Rab3B-binding domain of Gas8. These results suggest that Gas8 links Rab3B to microtubules in NIH 3T3 cells.
Nishimura
Noriyuki
N
Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan.
Araki
Kunihiko
K
Shinahara
Wakako
W
Nakano
Yumiko
Y
Nishimura
Kaho
K
Higashio
Hironori
H
Sasaki
Takuya
T
D000818:Animals; D015153:Blotting, Western; D006224:Cricetinae; D003598:Cytoskeletal Proteins; D047468:Immunoprecipitation; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D020692:rab3 GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
D003598:Cytoskeletal Proteins; C452182:Gas8 protein, mouse; D011506:Proteins; C116709:Rab3b protein, mouse; D020692:rab3 GTP-Binding Proteins