title: Mice deficient for a small cluster of Piwi-interacting RNAs implicate Piwi-interacting RNAs in transposon control.
abstract: The mammalian testis expresses a class of small noncoding RNAs that interact with mammalian PIWI proteins. In mice, the PIWI-interacting RNAs (piRNAs) partner with mammalian PIWI proteins, PIWIL1 and PIWIL2, also known as MIWI and MILI, to maintain transposon silencing in the germline genome. Here, we demonstrate that inactivation of Nct1/2, two noncoding RNAs encoding piRNAs, leads to derepression of LINE-1 (L1) but does not affect mouse viability, spermatogenesis, testicular gene expression, or fertility. These findings indicate that piRNAs from a cluster on chromosome 2 are necessary to maintain transposon silencing.
authors.affiliation: Center for Research on Reproduction and Women's Health, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6080, USA.
authors.forename: Mingang
authors.initials: M
authors.lastname: Xu
authors.forename: Yun
authors.initials: Y
authors.lastname: You
authors.forename: Patricia
authors.initials: P
authors.lastname: Hunsicker
authors.forename: Tamaki
authors.initials: T
authors.lastname: Hori
authors.forename: Chris
authors.initials: C
authors.lastname: Small
authors.forename: Michael D
authors.initials: MD
authors.lastname: Griswold
authors.forename: Norman B
authors.initials: NB
authors.lastname: Hecht
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D020868:Gene Silencing; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D005810:Multigene Family / Q000502:physiology; D009154:Mutation / Q000502:physiology; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000235:genetics* / Q000502:physiology*; D012098:Reproduction / Q000235:genetics; D013737:Testis / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D034741:RNA, Small Interfering