title: Efficacy of sanitizing treatments against Penicillium expansum inoculated on six varieties of apples.
abstract: The effectiveness of several wash treatments was evaluated against spores of Penicillium expansum inoculated on six varieties of apples (Red Delicious, Golden Supreme, Empire, Macintosh, Fuji, and Gala). The wash treatments were water, acidified water (pH 6.5), acidified sodium hypochlorite (pH 6.5), nonacidified sodium hypochlorite (pH 8.8, 9.3, and 9.7; 50, 100, and 200 ppm, respectively), and peracetic acid (50 and 80 ppm). Spores of P. expansum were dried on the surface of the apples for 2 h before exposure to the different sanitizer solutions. Each apple was submerged in 100 ml of each treatment solution for 30 s, and the number of spores remaining were recovered and enumerated. The efficacy of chlorine solutions was enhanced by decreasing the pH to 6.5 (up to 5-log reduction, depending on apple variety). Peracetic acid solutions (50 and 80 ppm) resulted in a reduction of less than 2 log spores per g and had the same efficacy (P < or = 0.05) as nonacidified chlorine solutions (50, 100, and 200 ppm). Control water solutions produced a reduction of 1.34 log spores per g. Chlorine solutions at pH 6.5 resulted in the largest reduction of P. expansum spores for all apple varieties tested.
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
authors.forename: Beatriz C M
authors.initials: BC
authors.lastname: Salomão
authors.forename: Gláucia M F
authors.initials: GM
authors.lastname: Aragão
authors.forename: John J
authors.initials: JJ
authors.lastname: Churey
authors.forename: Randy W
authors.initials: RW
authors.lastname: Worobo
mesh_terms: D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000382:microbiology*; D010407:Penicillium / Q000187:drug effects* / Q000254:growth & development; D010463:Peracetic Acid / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013172:Spores, Fungal / Q000187:drug effects; D014867:Water / Q000494:pharmacology
chemical_list: D004202:Disinfectants; D014867:Water; D012973:Sodium Hypochlorite; D010463:Peracetic Acid