Transformation by bovine papillomavirus type 1 E6 requires paxillin.
Papillomavirus E6 proteins are adapters that change the function of cellular regulatory proteins. The bovine papillomavirus type 1 E6 (BE6) binds to LXXLL peptide sequences termed LD motifs (consensus sequence LDXLLXXL) on the cellular protein paxillin that is a substrate of Src and focal adhesion kinases. Anchorage-independent transformation induced by BE6 required both paxillin and BE6-binding LD motifs on paxillin but was independent of the major tyrosine phosphorylation sites of paxillin. The essential role of paxillin in transformation by BE6 highlights the role of paxillin in the transduction of cellular signals that result in anchorage-independent cell proliferation.
Wade
Ramon
R
Department of Pathology, University of Virginia, P.O. Box 800904, Charlottesville, VA 22908-0904, USA.
Brimer
Nicole
N
Vande Pol
Scott
S
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002472:Cell Transformation, Viral; D016384:Consensus Sequence; D005982:Glutathione Transferase / Q000378:metabolism; D058619:Maltose-Binding Proteins; D009154:Mutation; D051419:Paxillin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D051419:Paxillin; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase