Resistin does not down-regulate the transcription of insulin receptor promoter.
OBJECTIVE
To detect the effect of resistin on the transcription of insulin receptor promoter.


METHODS
Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy.


RESULTS
The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus.


CONCLUSIONS
The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.
Qiao
Xiao-zhi
XZ
Department of Very Important Person, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. qiao.xiaozhi@163.com
Wang
Xian-feng
XF
Xu
Zhe-rong
ZR
Yang
Yun-mei
YM
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D018613:Microscopy, Confocal; D008954:Models, Biological; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011972:Receptor, Insulin / Q000096:biosynthesis* / Q000235:genetics*; D052243:Resistin / Q000494:pharmacology*; D014158:Transcription, Genetic; D014162:Transfection
D052243:Resistin; D008156:Luciferases; D011972:Receptor, Insulin