title: Cytochrome P450 1A activity in liver and fixed wavelength fluorescence detection of polycyclic aromatic hydrocarbons in the bile of tongue-fish (Cynoglossus acrolepidotus, Bleeker) in relation to petroleum hydrocarbons in the eastern Gulf of Thailand.
abstract: This investigation was conducted in an area of oil spill along the east coast of Thailand to examine the relations among cytochrome P450 1A activity in liver and PAHs in the bile of the tonguefish and petroleum hydrocarbons in the sediments. PAH sediment concentrations in the reference and oil spill areas were 5.03 +/- 0.42 and 0.21 +/- 0.043 microg(-1) dry weight respectively Cytochrome activity in fish liver from oil spill area was 45.40 +/- 3.50 pmoles/ min/mg protein, almost threefold higher than that from the reference sites. Flourescense detection in bile metabolites at the oil spill area, 69.8 +/- 9.9 flourescense unit was significantly higher than that at the reference sites, 22.9 +/- 5.5 and 22.2 +/- 3.5 fluorescence unit. A strong correlation was found among cytochrome P450 1A activity in liver, PAH of bile metabolites and petroleum hydrocarbons. Both cytochrome and bile metabolites activity decreased seaward varying to the distance from the oil polluted area. We concluded that both detections in tonguefish can be regarded as a complementary biomarkers for the exposure of PAHs in tropical marine environments.
authors.affiliation: Department of Aquatic Science, Faculty of Science, Burapha University, Chonburi-20131, Thailand. voravit@buu.ac.th
authors.forename: Voravit
authors.initials: V
authors.lastname: Cheevaporn
authors.forename: F William H
authors.initials: FW
authors.lastname: Beamish
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005399:Fishes; D019015:Geologic Sediments / Q000737:chemistry; D008099:Liver / Q000201:enzymology*; D010578:Petroleum / Q000032:analysis*; D011083:Polycyclic Compounds / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D010578:Petroleum; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System