title: A multi-angular mass spectrometric view at cyclic nucleotide dependent protein kinases: in vivo characterization and structure/function relationships.
abstract: Mass spectrometry has evolved in recent years to a well-accepted and increasingly important complementary technique in molecular and structural biology. Here we review the many contributions mass spectrometry based studies have made in recent years in our understanding of the important cyclic nucleotide activated protein kinase A (PKA) and protein kinase G (PKG). We both describe the characterization of kinase isozymes, substrate phosphorylation, binding partners and post-translational modifications by proteomics based methodologies as well as their structural and functional properties as revealed by native mass spectrometry, H/D exchange MS and ion mobility. Combining all these mass spectrometry based data with other biophysical and biochemical data has been of great help to unravel the intricate regulation of kinase function in the cell in all its magnificent complexity.
authors.affiliation: Biomolecular Mass Spectrometry & Proteomics Group, Utrecht Institute of Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Sorbonnelaan 16, 3584CA, Utrecht, The Netherlands.
authors.forename: Arjen
authors.initials: A
authors.lastname: Scholten
authors.forename: Thin-Thin
authors.initials: TT
authors.lastname: Aye
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017868:Cyclic AMP-Dependent Protein Kinases / Q000737:chemistry*; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000737:chemistry*; D040901:Proteomics; D013329:Structure-Activity Relationship
chemical_list: D007527:Isoenzymes; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C