title: [Experimental pancreatic islet transplant into the genito-urinary tract simultaneous to kidney transplant].
abstract: OBJECTIVE
  Simultaneous kidney and pancreas transplant is a good treatment for both renal and pancreas insufficiency. Experimental apply of genitourinary tract for pancreas implantation is reported in this work.
  
  
  METHODS
  Twenty animals aged as average 5.5 monts (SD 1.1) and an average weight of 53 kgr were submitted to this protocol. In the day 1 a left nephrectomy is completed and the graft is perfused with University of Wisconsin solution. A partial pancreatectomy is completed at following, isolation of pancreatic islets by colagenase enzymatic digestion. Islets are dryed with Ditizone and culptured for 24 hours at 37 degrees C and 5% CO2. Day-2 a right nephrectomy is performed and orthotopic renal autotransplant using the left kidney is completed. Pancreatic islets are transplanted in 4 different locations of the genitourinary tract: renal subcapsular space, bladder submucosae, testis parenchyma and vas deferens. Day-7, all the animals were sacrifized to complete pathological study.
  
  
  CONCLUSIONS
  Viable islets were isolated in bladder submucosae and testis after transdeferential injection.
authors.affiliation: Unidad de Urología, Fundación Hospital Alcorcón, Madrid. urologo@tiscali.es
authors.forename: V
authors.initials: V
authors.lastname: Gómez Dos Santos
authors.forename: F J
authors.initials: FJ
authors.lastname: Burgos Revilla
authors.forename: J
authors.initials: J
authors.lastname: Pascual Santos
authors.forename: R
authors.initials: R
authors.lastname: Marcen Letosa
authors.forename: J J
authors.initials: JJ
authors.lastname: Villafruela Gómez
authors.forename: C
authors.initials: C
authors.lastname: Correa Gorospe
authors.forename: B
authors.initials: B
authors.lastname: Cuevas Muñoz
authors.forename: F
authors.initials: F
authors.lastname: Mampaso
authors.forename: R
authors.initials: R
authors.lastname: García Gonzalez
mesh_terms: D000818:Animals; D050417:Insulin-Secreting Cells / Q000637:transplantation*; D016030:Kidney Transplantation; D013552:Swine; D016042:Transplantation, Heterotopic / Q000379:methods*; D014566:Urogenital System / Q000601:surgery*