The proteome of gastric lymph in normal and nematode infected sheep.
Lymph node cannulation allows the collection of lymph draining from a defined anatomical region. Proteomic analysis of that lymph offers a potentially valuable insight into the immunoinflammatory response of that particular region. In this study, ovine gastric lymph has been used to monitor the proteomic changes occurring in the tissue fluid of the abomasum, in response to infection with the parasitic nematode, Teladorsagia circumcincta. Lymph, collected temporally over an experimental infection period, was analysed by means of 2-DE and subsequent gel analysis using densitometry software. In addition, the composition of the lymphatic proteome was further explored by means of MALDI-TOF and MS/MS analyses. The concentration of gelsolin, alpha-1 beta glycoprotein and haemopexin were altered significantly (p<0.05) with infection.
Goldfinch
Gillian M
GM
Division of Veterinary Clinical Studies, University of Edinburgh, Easter Bush, Veterinary Centre, Edinburgh, UK. Gillian.goldfinch@moredun.ac.uk
Smith
W David
WD
Imrie
Lisa
L
McLean
Kevin
K
Inglis
Neil F
NF
Pemberton
Alan D
AD
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003720:Densitometry / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D006376:Helminths / Q000378:metabolism*; D007107:Immune System; D007074:Immunoglobulin G / Q000737:chemistry; D008196:Lymph / Q000469:parasitology*; D008198:Lymph Nodes / Q000469:parasitology*; D013058:Mass Spectrometry / Q000379:methods; D009348:Nematoda / Q000378:metabolism*; D009349:Nematode Infections / Q000378:metabolism* / Q000662:veterinary*; D040901:Proteomics / Q000379:methods; D012756:Sheep; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007074:Immunoglobulin G