title: An improved PCR method for gender identification of eagles.
abstract: Eagles are sexually monomorphic and therefore it is difficult to determine their gender, which is a crucial need for management purposes. In this study, we have developed an improved gender identification method by exploiting length differences between the Chromo-Helicase-DNA binding protein (CHD)-Z and CHD-W genes of Spilornis cheela hoya. By comparing DNA sequences for CHD-W and CHD-Z from 10 species of Falconiformes eagles we designed universal gender identification PCR primers that exploit differences in product size. Standard agarose gels were shown to easily distinguish between the 148-bp CHD-ZW and the 258-bp CHD-W PCR products. When used with 28 samples of S. cheela hoya, our improved universal primers provided a fast and precise gender identification assay.
authors.affiliation: Faculty of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan. changhw@kmu.edu.tw
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Ta-Ching
authors.initials: TC
authors.lastname: Chou
authors.forename: De-Leung
authors.initials: DL
authors.lastname: Gu
authors.forename: Chun-An
authors.initials: CA
authors.lastname: Cheng
authors.forename: Chia-Che
authors.initials: CC
authors.lastname: Chang
authors.forename: Cheng-Te
authors.initials: CT
authors.lastname: Yao
authors.forename: Li-Yeh
authors.initials: LY
authors.lastname: Chuang
authors.forename: Cheng-Hao
authors.initials: CH
authors.lastname: Wen
authors.forename: Yii-Cheng
authors.initials: YC
authors.lastname: Chou
authors.forename: Kock-Yee
authors.initials: KY
authors.lastname: Tan
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D020307:Eagles / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis; D013045:Species Specificity
chemical_list: D017931:DNA Primers