Alterations in the mechanical properties of bladder smooth muscle in hydrocephalus rat model.
OBJECTIVE
It is now well established that hydrocephalus is associated with impaired bladder function. The aim of this study was to determine the effects of hydrocephalus on bladder smooth muscle (BSM) reactivity in the rat model.


METHODS
Hydrocephalus was induced in 7-day-old rats by injection of kaolin into the cisterna magna (AH group). Control group rats underwent a sham operation. After 10 days, rats were decapitated. Each bladder was excised and BSM strips placed in an organ bath where contractile and relaxant responses were studied.


RESULTS
Contractile response of BSM to KCl decreased in the AH group. Increased response to muscarinic agonist carbachol was observed in the AH group. The relaxant response to adrenergic agonist isoprenaline was significantly decreased in the AH group, whereas non-receptor-dependent agonist papaverine was unchanged in 2 groups.


CONCLUSIONS
Bladder smooth muscle reactivity is affected by the formation of hydrocephalus essentially by both receptor-dependent and non-receptor-dependent mechanisms. This pathway may be a novel target for the pharmacologic treatment of bladder dysfunction secondary to hydrocephalus.
Tugay
Melih
M
Department of Pediatric Surgery, Medical Faculty of Kocaeli University, Kocaeli 41380, Turkey. tugaym@hotmail.com
Tugay
Sevinç
S
Etuş
Volkan
V
Yazir
Yusufhan
Y
Utkan
Tijen
T
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D001696:Biomechanical Phenomena; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006849:Hydrocephalus / Q000150:complications*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000473:pathology / Q000503:physiopathology*; D010208:Papaverine / Q000494:pharmacology; D051381:Rats; D001743:Urinary Bladder / Q000473:pathology / Q000503:physiopathology*; D001745:Urinary Bladder Diseases / Q000209:etiology / Q000473:pathology / Q000503:physiopathology
D000318:Adrenergic beta-Agonists; D010208:Papaverine; D007545:Isoproterenol