Analysis of interleukin-8, interleukin-10, and tumor necrosis factor-alpha in the cerebrospinal fluid of patients with cervical spondylotic myelopathy.
METHODS
Retrospective cohort study.


OBJECTIVE
The expression of interleukin-8 (IL-8), IL-10, and tumor necrosis factor-alpha (TNF-alpha) were measured in the cerebrospinal fluid (CSF) of patients with cervical myelopathy. The purpose of this study was to examine whether the CSF levels of those 3 cytokines differ significantly among 3 groups of patients with different diseases.


METHODS
IL-8, IL-10, and TNF-alpha levels were analyzed using enzyme-linked immune assay. CSF samples were collected from 3 groups of patients. The cervical spondylotic myelopathy (CSM) group consisted of 35 patients. The ossification of the posterior longitudinal ligament group [(OPLL) group] consisted of 7 patients, and the control group consisted of 12 patients. The concentration of IL-8 was 69.0+/-35.2 pg/mL in the CSM group, 82.1+/-46.7 pg/mL in the OPLL group, and 43.5+/-20.9 pg/mL in the control group. The concentration of IL-8 was significantly higher in the CSM and OPLL groups than in the control group (P<0.05). There was no significant difference between the CSM group and OPLL group. The concentration of IL-10 and TNF-alpha in all groups was below the sensitivity of the measurements.


CONCLUSIONS
In this study, the concentration of IL-8 was high in CSM and OPLL patients. However, the concentration of IL-10 and TNF-alpha was below the sensitivity of the measurements.
Ito
Keigo
K
Department of Orthopedic Surgery, Chubu Rosai Hospital, Nagoya University School of Medicine, Nagoya, Aichi, Japan.
Matsuyama
Yukihiro
Y
Yukawa
Yasutsugu
Y
Kato
Fumihiko
F
Ishiguro
Naoki
N
D000328:Adult; D000368:Aged; D002574:Cervical Vertebrae; D015331:Cohort Studies; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000134:cerebrospinal fluid*; D016209:Interleukin-8 / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D017887:Ossification of Posterior Longitudinal Ligament / Q000134:cerebrospinal fluid; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D013118:Spinal Cord Diseases / Q000134:cerebrospinal fluid*; D013128:Spinal Osteophytosis / Q000134:cerebrospinal fluid*; D014409:Tumor Necrosis Factor-alpha / Q000134:cerebrospinal fluid*
D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10