Synthesis and in vivo studies of carbohydrate-based vaccines against group A streptococcus.
Carbohydrates, as carriers, providing numerous attachment points for the conjugation of peptide antigens and their optimal orientation for the recognition by cells of the immune system, reducing degradation of the attached peptide antigens and many other advantages make carbohydrate-based vaccine highly promising approach. Multiple copies of a single group A streptococcal (GAS) M protein derived specific peptide antigens (J8 or J14) were coupled onto carbohydrate cores (D-glucose and D-galactose) linked to lipophilic amino acids to produce a self-adjuvanting liposaccharide vaccine against GAS strains. In vivo experiments showed high serum IgG antibody titers against each of the incorporated peptide epitopes, J8 or J14.
Simerska
Pavla
P
School of Molecular and Microbial Sciences (SMMS), The University of Queensland, St Lucia, Queensland 4072, Australia. p.simerska@uq.edu.au
Abdel-Aal
Abu-Baker M
AB
Fujita
Yoshio
Y
Batzloff
Michael R
MR
Good
Michael F
MF
Toth
Istvan
I
D000181:Acrylonitrile / Q000008:administration & dosage / Q000138:chemical synthesis; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000138:chemical synthesis; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002240:Carbohydrate Sequence; D004337:Drug Carriers / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D005690:Galactose / Q000008:administration & dosage / Q000737:chemistry*; D005947:Glucose / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000235:genetics / Q000276:immunology; D013290:Streptococcal Infections / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000276:immunology; D013297:Streptococcus pyogenes / Q000235:genetics / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D004337:Drug Carriers; D000939:Epitopes; D010455:Peptides; D022541:Streptococcal Vaccines; D005947:Glucose; D000181:Acrylonitrile; D005690:Galactose