title: Are locus coeruleus neurons involved in blinking?
abstract: To investigate the involvement of the noradrenergic locus coeruleus (LC) in the reflex blink circuit, c-Fos and neuronal tracer experiments were performed in the rat. LC neurons involved in reflex blink were localized by analyzing c-Fos protein expression after electrical stimulation of the supraorbital nerve. Subsequently, neuronal tracers were injected in two different nuclei which are part of the reflex blink circuit. Anterograde tracer experiments in the sensory trigeminal complex (STC) explored the trigemino-coerulear connection; retrograde tracer experiments in the latero-caudal portion of the superior colliculus (SC) established coerulear-collicular connections. The combination of retrograde tracer injections into the latero-caudal SC portion combined with electrical stimulation of the supraorbital nerve identified c-Fos positive LC neurons that project to the latero-caudal SC. Our results revealed the existence of a STC-LC-SC loop.
authors.affiliation: Laboratoire de Physiologie de la Manducation, UP7, 4 place Jussieu, 75252 Paris Cedex 05, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Dauvergne
authors.forename: Albertine Ellen
authors.initials: AE
authors.lastname: Smit
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Valla
authors.forename: Monique
authors.initials: M
authors.lastname: Diagne
authors.forename: Catherine
authors.initials: C
authors.lastname: Buisseret-Delmas
authors.forename: Pierre
authors.initials: P
authors.lastname: Buisseret
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Pinganaud
authors.forename: Frans
authors.initials: F
authors.lastname: Vanderwerf
mesh_terms: D000344:Afferent Pathways / Q000166:cytology* / Q000502:physiology; D000818:Animals; D001767:Blinking / Q000502:physiology*; D005143:Eyelids / Q000294:innervation*; D007150:Immunohistochemistry; D008125:Locus Coeruleus / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D051381:Rats