Determination of beta-carboline alkaloids in fruiting bodies of Hygrophorus spp. by liquid chromatography/electrospray ionisation tandem mass spectrometry.
The beta-carboline alkaloids harmane (1) and norharmane (2) were isolated from fruiting bodies of Hygrophorus eburneus (Bull.) Fr. as well as brunnein A (3) from Hygrophorus hyacinthinus Quél. (Tricholomataceae, Agaricales) for the first time. Their occurrence within the genus was investigated using liquid chromatography/electrospray ionisation tandem mass spectrometric methods, especially by selected reaction monitoring. Based on these results their chemotaxonomical relevance is discussed.
Teichert
Axel
A
Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany.
Lübken
Tilo
T
Schmidt
Jürgen
J
Kuhnt
Christine
C
Huth
Manfred
M
Porzel
Andrea
A
Wessjohann
Ludger
L
Arnold
Norbert
N
D000363:Agaricales / Q000737:chemistry*; D000470:Alkaloids / Q000032:analysis*; D002243:Carbolines / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000470:Alkaloids; D002243:Carbolines