Cloud point extraction and determination of trace trichlorfon by high performance liquid chromatography with ultraviolet-detection based on its catalytic effect on benzidine oxidizing.
4-Amino-4'-nitrobiphenyl, which is formed by catalytic effect of trichlorfon on sodium perborate oxidizing benzidine, is extracted with a cloud point extraction method and then detected using a high performance liquid chromatography with ultraviolet detection (HPLC-UV). Under the optimum experimental conditions, there was a linear relationship between trichlorfon in the concentration range of 0.01-0.2 mgL(-1) and the peak areas of 4-amino-4'-nitrobiphenyl (r=0.996). Limit of detection was 2.0 microgL(-1), recoveries of spiked water and cabbage samples ranged between 95.4-103 and 85.2-91.2%, respectively. It was proved that the cloud point extraction (CPE) method was simple, cheap, and environment friendly than extraction with organic solvents and had more effective extraction yield.
Zhu
Hai-Zhen
HZ
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
Liu
Wei
W
Mao
Jian-Wei
JW
Yang
Ming-Min
MM
D001560:Benzidines / Q000737:chemistry*; D002138:Calibration; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014236:Trichlorfon / Q000032:analysis*
D001560:Benzidines; C029876:benzidine; D014236:Trichlorfon