title: Flow cytometric analysis of canine umbilical cord blood lymphocytes.
abstract: Lymphocyte subsets in canine umbilical cord blood were flow cytometrically analyzed and compared with those of the dams' peripheral blood. The proportion of CD3+ T lymphocytes, CD21+CD3- B lymphocytes, and CD3-CD21- non-T non-B lymphocytes in umbilical cord blood was 52.9%, 30.4%, and 16.7%, respectively. T lymphocyte/B lymphocyte ratio was significantly lower in the umbilical cord blood than in the dams' peripheral blood (2.1 +/- 1.4 versus 11.0 +/- 8.1, P < 0.001). In contrast, CD4+ lymphocyte/CD8+ lymphocyte ratio was significantly higher in the umbilical cord blood than in the dams' peripheral blood (7.6 +/- 2.2 versus 1.8 +/- 0.6, P<0.001). These findings clarified the phenotypic characters of canine umbilical cord blood lymphocytes.
authors.affiliation: Laboratory of Veterinary Physiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan. otani@brs.nihon-u.ac.jp
authors.forename: Isao
authors.initials: I
authors.lastname: Otani
authors.forename: Kohei
authors.initials: K
authors.lastname: Ohta
authors.forename: Akira
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Takeki
authors.initials: T
authors.lastname: Yamada
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Ishinazaka
authors.forename: Tadatoshi
authors.initials: T
authors.lastname: Ohtaki
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Tsumagari
authors.forename: Kiichi
authors.initials: K
authors.lastname: Kanayama
mesh_terms: D000818:Animals; D016516:CD4-CD8 Ratio; D004285:Dogs / Q000097:blood* / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D008214:Lymphocytes / Q000097:blood*