Application of a modified Haug and Lantzsch method for the rapid and accurate photometrical phytate determination in soybean, wheat, and maize meals.
A modified version of the Haug and Lantzsch method for rapid photometrical phytate determination was applied for the analysis of phytate in soybean, wheat, and maize meals. In comparison to the original protocol, the amount of the toxic reagent thioglycolic acid is reduced substantially to minimize potential health risks for laboratory personnel. Different extraction conditions for soybean meal were tested, and boiling for at least 30 min was found to be necessary to remove an interfering compound in soybean meal extracts. Phytate contents determined according to the modified Haug and Lantzsch method did not differ from those obtained by measuring total precipitated phosphorus or by sensitive and specific high-performance ion chromatography. Applicability and accuracy of the modified method for phytate analysis in major feed substrates, including soy-based textured vegetable protein, were demonstrated.
Reichwald
Kirsten
K
Novozymes A/S, Laurentsvej 55, DK-2880 Bagsvaerd, Denmark. kire@dsr.life.ku.dk
Hatzack
Frank
F
D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D010783:Photometry / Q000379:methods*; D010833:Phytic Acid / Q000032:analysis*; D012639:Seeds / Q000737:chemistry; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*; D013864:Thioglycolates; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D007202:Indicators and Reagents; D013864:Thioglycolates; D010833:Phytic Acid