title: [The establishment and assessment on cosmetic products using 3T3 mouse fibroblast neutral red uptake phototoxicity assay].
abstract: OBJECTIVE
  To establish the 3T3 mouse fibroblast neutral red uptake (NRU-PT) phototoxicity test method, and evaluate the practicality of the method in detecting potential phototoxicity of the cosmetic products.
  
  
  METHODS
  Fifteen phototoxic and 9 non-phototoxic chemicals were tested in our laboratories, the phototoxic potential of the test chemicals was evaluated in a prediction model in which either the photo irritation factor (PIF) or the mean photo effect (MPE) was compared with the coherence and sensitivity of the method. 20 kinds of functional cosmetics were detected and the results were analyzed by the 3T3 NRU-PT in vitro and Guinea pig skin phototoxicity test (in vivo).
  
  
  RESULTS
  Both PIF and MPE of the chemicals were highly reproduced, and the correlation between in vitro and in vivo data was almost perfect. All the non-phototoxic provided a negative result, while 14 of the 15 phototoxic tested chemicals gave clear positive results. For cosmetics, the correlation between in vitro and in vivo data was consistent.
  
  
  CONCLUSIONS
  The 3T3 NRU PT test was established successfully, it should be used as a good alternative method for assessing the phototoxic potential of the chemicals and cosmetics in China.
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510030, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Xi-Kun
authors.initials: XK
authors.lastname: Xiong
authors.forename: Xing-Fen
authors.initials: XF
authors.lastname: Yang
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.forename: Jun-Ming
authors.initials: JM
authors.lastname: Huang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Tan
authors.forename: Sui-Sheng
authors.initials: SS
authors.lastname: Zheng
mesh_terms: D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D003358:Cosmetics / Q000633:toxicity*; D017484:Dermatitis, Phototoxic; D005347:Fibroblasts / Q000187:drug effects*; D006168:Guinea Pigs; D051379:Mice; D018675:Toxicity Tests
chemical_list: D003358:Cosmetics