title: Predicting left ventricular remodeling after a first myocardial infarction by plasma proteome analysis.
abstract: Recent improvements in therapeutic strategies did not prevent left ventricular remodeling (LVR), which remains a common event (30%) after acute myocardial infarction (AMI). We report the use of a systematic approach, based on comparative proteomics, to select circulating biomarkers that may be associated with LVR. We selected 93 patients enrolled in a prospective study. These patients with anterior wall Q-wave AMI underwent echocardiographic follow-up at hospitalization, 3 months and 1 year after AMI. They were divided into three groups (no, low, or high remodeling). Plasma samples of these patients (day 5 of hospitalization) were processed and stored at -80 degrees C within 2 h and analyzed using SELDI-TOF protein chip technology. This systematic approach allowed to select candidate proteins modulated by LVR: post-translational variants of alpha1-chain of haptoglobin (Hpalpha1) corresponding to m/z 9493, 9565, and 9623, which were more elevated in remodeling patients. The peak 9493 m/z was shown having a receiving-operating characteristic (ROC) value of 0.71 between non- and remodeling patients. SELDI-TOF approach may lead to the identification of circulating proteins associated with LVR. Whether these candidate proteins will help to identify patients who are at high risk of heart failure after AMI will have to be tested in future studies.
authors.affiliation: INSERM U744, Lille, France. florence.pinet@pasteur-lille.fr
authors.forename: Florence
authors.initials: F
authors.lastname: Pinet
authors.forename: Olivia
authors.initials: O
authors.lastname: Beseme
authors.forename: Caroline
authors.initials: C
authors.lastname: Cieniewski-Bernard
authors.forename: Hervé
authors.initials: H
authors.lastname: Drobecq
authors.forename: Sabine
authors.initials: S
authors.lastname: Jourdain
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lamblin
authors.forename: Philippe
authors.initials: P
authors.lastname: Amouyel
authors.forename: Christophe
authors.initials: C
authors.lastname: Bauters
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods; D004452:Echocardiography / Q000379:methods; D005260:Female; D006242:Haptoglobins / Q000096:biosynthesis / Q000737:chemistry*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D012372:ROC Curve; D020257:Ventricular Remodeling
chemical_list: D001798:Blood Proteins; D006242:Haptoglobins