Influence of ionic strength on Hoechst 33258 binding with DNA.
The binding of Hoechst 33258 with DNA at various ionic strengths of solution and different ligand concentrations has been investigated. Existence of more than one type of interactions of Hoechst 33258 with DNA has been revealed, which were very sensitive to the ionic strength. Hoechst 33258 doesn't show specificity to AT sequences of DNA at low ionic strength. High affinity binding mode becomes obvious at high ionic strength. The values of binding constants and binding site sizes for revealed strong and weak interactions have been determined.
Vardevanyan
Poghos O
PO
Department of Biophysics, Yerevan State University, Yerevan, 375025, Armenia.
Antonyan
Ara P
AP
Parsadanyan
Marine A
MA
Pirumyan
Kristine V
KV
Muradyan
Andranik M
AM
Karapetian
Armen T
AT
D001665:Binding Sites; D006690:Bisbenzimidazole / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D013816:Thermodynamics
D005456:Fluorescent Dyes; D004247:DNA; D006690:Bisbenzimidazole