Syntaxin 6 regulates nerve growth factor-dependent neurite outgrowth.
Neurite outgrowth is crucial for neural circuit formation. Intracellular membrane trafficking is involved in the cell surface expansion that is necessary for neurite outgrowth. It is known that syntaxin 6 is predominantly located in the Golgi region in undifferentiated PC12 cells and that it regulates trans-Golgi network trafficking and the secretory pathway via its coiled-coil domains. However, whether it also regulates neurite outgrowth remains unknown. In this paper, we found that syntaxin 6 was located both in the Golgi apparatus and the distal tips of the neurites of nerve growth factor (NGF)-treated PC12 cells. We also showed that the overexpression of the first coiled-coil domain of syntaxin 6 inhibited NGF-dependent neurite outgrowth. However, the coiled-coil domain-disrupting mutant had little effect on neurite outgrowth. These results suggest that the first coiled-coil domain of syntaxin 6 plays a crucial role in NGF-dependent neurite outgrowth.
Kabayama
Hiroyuki
H
Laboratory for Developmental Neurobiology, Brain Science Institute, Institute of Physical and Chemical Research RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kabayama@brain.riken.jp
Tokushige
Naoko
N
Takeuchi
Makoto
M
Mikoshiba
Katsuhiko
K
D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D016716:PC12 Cells / Q000648:ultrastructure; D017434:Protein Structure, Tertiary / Q000502:physiology; D050765:Qa-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014162:Transfection
D050765:Qa-SNARE Proteins; D020932:Nerve Growth Factor