title: Coupling of fully automated chip-based electrospray ionization to high-capacity ion trap mass spectrometer for ganglioside analysis.
abstract: NanoMate robot was coupled to a high-capacity ion trap (HCT) mass spectrometer to create a system merging automatic chip-based electrospray ionization (ESI) infusion, ultrafast ion detection, and multistage sequencing at superior sensitivity. The interface between the NanoMate and HCT mass spectrometer consists of an in-laboratory constructed mounting device that allows adjustment of the robot position with respect to the mass spectrometer inlet. The coupling was optimized for ganglioside (GG) high-throughput analysis in the negative ion mode and was implemented in clinical glycolipidomics for identification and structural characterization of anencephaly-associated species. By NanoMate HCT mass spectrometry (MS), data corroborating significant differences in GG expression in anencephalic versus age-matched normal brain tissue were collected. The feasibility of chip-based nanoESI HCT multistage collision-induced dissociation (CID MS(n)) for polysialylated GG fragmentation and isomer discrimination was tested on a GT1 (d18:1/18:0) anencephaly-associated structure. MS(2)-MS(4) obtained by accumulating scans at variable fragmentation amplitudes gave rise to the first fragmentation patterns from which the presence of GT1b structural isomer could be determined unequivocally without the need for supplementary investigation by any other analytical or biochemical methods.
authors.affiliation: Advion Biosciences, Norfolk, Norwich NR9 3DB, UK.
authors.forename: Reinaldo
authors.initials: R
authors.lastname: Almeida
authors.forename: Cristina
authors.initials: C
authors.lastname: Mosoarca
authors.forename: Marius
authors.initials: M
authors.lastname: Chirita
authors.forename: Valentina
authors.initials: V
authors.lastname: Udrescu
authors.forename: Nicolae
authors.initials: N
authors.lastname: Dinca
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Vukelić
authors.forename: Mark
authors.initials: M
authors.lastname: Allen
authors.forename: Alina D
authors.initials: AD
authors.lastname: Zamfir
mesh_terms: D001331:Automation; D005732:Gangliosides / Q000032:analysis* / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*
chemical_list: D005732:Gangliosides; D007477:Ions