Detection of active butyrate-degrading microorganisms in methanogenic sludges by RNA-based stable isotope probing.
Butyrate-degrading bacteria in four methanogenic sludges were studied by RNA-based stable isotope probing. Bacterial populations in the (13)C-labeled rRNA fractions were distinct from unlabeled fractions, and Syntrophaceae species, Tepidanaerobacter sp., and Clostridium spp. dominated. These results suggest that diverse microbes were active in butyrate degradation under methanogenic conditions.
Hatamoto
Masashi
M
Department of Environmental Systems Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Imachi
Hiroyuki
H
Yashiro
Yuto
Y
Ohashi
Akiyoshi
A
Harada
Hideki
H
D002087:Butyrates / Q000378:metabolism*; D002247:Carbon Isotopes / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification* / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000302:isolation & purification*; D007553:Isotope Labeling; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D002087:Butyrates; D002247:Carbon Isotopes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008697:Methane