[Genetic polymorphisms of five STR gene loci GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05 alleles in Chinese Han population of Chengdu area].
OBJECTIVE
To get the preliminary genotype and allele frequency distributions of GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05 loci in Chinese Han population in Chengdu area, and to validate more short tandem repeat (STR) systems for forensic application.


METHODS
The polymerase chain reaction (PCR) was used to this study. Five STRs (GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05) were amplified from DNA samples, which were extracted with Chelex-100 method from EDTA-blood of 100 unrelated individuals. The PCR products were analyzed by PAG vertical electrophoresis.


RESULTS
No deviations from Hardy-Weinberg equilibrium were observed. The expected heterozygosities observed were 0.694, 0.918, 0.836, 0.889 and 0.880 for GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05 respectively. The discriminating powers were 0.697, 0.901, 0.875, 0.900 and 0.891.


CONCLUSIONS
The last four loci in this study are useful markers for genetics purposes in individual identification and paternity test.
Song
Yan-hua
YH
Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Chen
Xiao
X
Li
Ying
Y
Long
Bing
B
Chen
Guo-di
GD
D044466:Asian People / Q000235:genetics*; D002681:China / Q000208:ethnology; D005554:Forensic Medicine; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D005819:Genetic Markers