title: Designing an automated blood fractionation system.
abstract: BACKGROUND
  UK Biobank will be collecting blood samples from a cohort of 500 000 volunteers and it is expected that the rate of collection will peak at approximately 3000 blood collection tubes per day. These samples need to be prepared for long-term storage. It is not considered practical to manually process this quantity of samples so an automated blood fractionation system is required.
  
  
  METHODS
  Principles of industrial automation were applied to the blood fractionation process leading to the requirement of developing a vision system to identify the blood fractions within the blood collection tube so that the fractions can be accurately aspirated and dispensed into micro-tubes. A prototype was manufactured and tested on a range of human blood samples collected in different tube types.
  
  
  RESULTS
  A specially designed vision system was capable of accurately measuring the position of the plasma meniscus, plasma/buffy coat interface and the red cells/buffy coat interface within a vacutainer. A rack of 24 vacutainers could be processed in <5 min.
  
  
  CONCLUSIONS
  An automated, high throughput blood fractionation system offers a solution to the problem of processing human blood samples collected in vacutainers in a consistent manner and provides a means of ensuring data and sample integrity.
authors.affiliation: RTS Life Science Limited, Northbank, Irlam, Manchester M44 5AY, UK. adrian.mcquillan@rts-group.com
authors.forename: Adrian C
authors.initials: AC
authors.lastname: McQuillan
authors.forename: Sean D
authors.initials: SD
authors.lastname: Sales
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation; D001331:Automation / Q000592:standards*; D018070:Biological Specimen Banks; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D001793:Blood Preservation / Q000295:instrumentation; D001800:Blood Specimen Collection / Q000295:instrumentation*; D004867:Equipment Design; D006801:Humans; D017410:Practice Guidelines as Topic; D006113:United Kingdom