title: New efficient replication system with hepatitis C virus genome derived from a patient with acute hepatitis C.
abstract: We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-gamma and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-gamma are due to the difference in HCV strains, but not the cellular environment.
authors.affiliation: Department of Molecular Biology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Mori
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Abe
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Dansako
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ariumi
authors.forename: Masanori
authors.initials: M
authors.lastname: Ikeda
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kato
mesh_terms: D044966:Anti-Retroviral Agents / Q000494:pharmacology*; D001681:Biological Assay; D002460:Cell Line; D002999:Clone Cells; D016679:Genome, Viral; D015698:Genomic Library; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D006526:Hepatitis C / Q000821:virology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012093:Replicon / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
chemical_list: D044966:Anti-Retroviral Agents; D012313:RNA; D007371:Interferon-gamma