title: Syntaxin 6 regulates nerve growth factor-dependent neurite outgrowth.
abstract: Neurite outgrowth is crucial for neural circuit formation. Intracellular membrane trafficking is involved in the cell surface expansion that is necessary for neurite outgrowth. It is known that syntaxin 6 is predominantly located in the Golgi region in undifferentiated PC12 cells and that it regulates trans-Golgi network trafficking and the secretory pathway via its coiled-coil domains. However, whether it also regulates neurite outgrowth remains unknown. In this paper, we found that syntaxin 6 was located both in the Golgi apparatus and the distal tips of the neurites of nerve growth factor (NGF)-treated PC12 cells. We also showed that the overexpression of the first coiled-coil domain of syntaxin 6 inhibited NGF-dependent neurite outgrowth. However, the coiled-coil domain-disrupting mutant had little effect on neurite outgrowth. These results suggest that the first coiled-coil domain of syntaxin 6 plays a crucial role in NGF-dependent neurite outgrowth.
authors.affiliation: Laboratory for Developmental Neurobiology, Brain Science Institute, Institute of Physical and Chemical Research RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. kabayama@brain.riken.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kabayama
authors.forename: Naoko
authors.initials: N
authors.lastname: Tokushige
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Mikoshiba
mesh_terms: D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism; D009154:Mutation / Q000502:physiology; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D016716:PC12 Cells / Q000648:ultrastructure; D017434:Protein Structure, Tertiary / Q000502:physiology; D050765:Qa-SNARE Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D014162:Transfection
chemical_list: D050765:Qa-SNARE Proteins; D020932:Nerve Growth Factor