Capillary electrophoresis of sugar acids.
This chapter illustrates the usefulness of capillary electrophoresis (CE) for the analysis of sugar acids, that is, monosaccharides and lower oligosaccharides carrying carboxylate, sulphate or phosphate groups. In order to provide a general description of the main results and challenges in the field, some relevant applications and reviews on CE of such saccharidic compounds are tabulated. Furthermore, some detailed experimental procedures are shown, regarding the CE analysis of sugar acids released upon hydrolysis of acidic polysaccharides and of glycans linked to glycoproteins. In particular, the protocols will deal with the following compounds: (i) unsaturated, underivatized oligosaccharides from lyase-treated alginate; (ii) oligosaccharides derivatized with 4-aminobenzonitrile, arising from chemical hydrolysis of alginate; (iii) sialic acid derivatized with 2-aminoacridone, released from human serum immunoglobulin G.
Campa
Cristiana
C
Novartis Vaccines and Diagnostics, Siena, Italy.
Baiutti
Edi
E
Flamigni
Anna
A
D000464:Alginates / Q000737:chemistry; D020374:Chromatography, Micellar Electrokinetic Capillary; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000737:chemistry; D013400:Sugar Acids / Q000032:analysis* / Q000737:chemistry
D000464:Alginates; D007074:Immunoglobulin G; D013400:Sugar Acids; D019158:N-Acetylneuraminic Acid