Effect of anaesthesia-induced alterations in haemodynamics on in vivo kinetics of nitroxyl probes in electron spin resonance spectroscopy.
Although the advent of in vivo electron spin resonance (ESR) spectroscopy has allowed analysis of the redox status of living animals, whether the haemodynamic condition affects the signal decay rate remains unknown. Three kinds of haemodynamic conditions were generated by changing the anaesthetic dosage in mice. Haemodynamics was analysed (n=6 each) and in vivo ESR was performed to measure the signal decay rates of three nitroxyl spin probes (carbamoyl-, carboxy- and methoxycarbonyl-PROXYL) at the chest and head regions (n=6 for each condition and probe). Haemodynamic analysis revealed negative inotropic and chronotropic effects on the cardiovascular system depending on the depth of anaesthesia. Although signal decay rates differed among three probes, they were not affected by heart rate alteration. In this study we report the haemodynamics-independent signal decay rate of nitoxyl probes.
Tsutsumi
Takaki
T
Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Ide
Tomomi
T
Yamato
Mayumi
M
Andou
Makoto
M
Shiba
Takeshi
T
Utsumi
Hideo
H
Sunagawa
Kenji
K
D000758:Anesthesia / Q000379:methods*; D000818:Animals; D001835:Body Weight; D002318:Cardiovascular Diseases / Q000378:metabolism; D004562:Electrocardiography / Q000379:methods; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D006339:Heart Rate; D006439:Hemodynamics; D007700:Kinetics; D008297:Male; D051379:Mice; D009589:Nitrogen Oxides / Q000737:chemistry*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species; D013997:Time Factors
D009589:Nitrogen Oxides; D017382:Reactive Oxygen Species; C039900:nitroxyl