Promoting of wakefulness by administrations of modafinil into anterior hypothalamus and into the pedunculopontine tegmental nucleus in rats.
We investigated whether administration of MOD in rats during the lights-on period into wake-promoting areas, such as anterior hypothalamus (AH) or into the pedunculopontine tegmental nucleus (PPTg) would enhance waking. Results showed that microinjections of 1 microL of MOD (10, 20, or 30 microg) into both brain areas increased the total time of alertness and decreased sleep. Additionally, MOD-treated rats showed an enhancement in alpha power spectra but delta power spectra was diminished. Finally, c-Fos expression was found increased into either AH or the PPTg. Collectively, these results suggest that MOD induces waking via the activity of two wake-related brain areas such as AH and the PPTg.
Murillo-Rodríguez
Eric
E
Laboratorio de Neurobiología, Facultad de Medicina, Universidad Autónoma de Campeche, Campeche, Mexico. emurillo@uacam.mx
Vázquez-Luis
Edgar
E
Millán-Aldaco
Diana
D
Haro
Reyes
R
Drucker-Colín
René
R
D000704:Analysis of Variance; D000818:Animals; D007025:Anterior Hypothalamic Nucleus / Q000187:drug effects*; D001522:Behavior, Animal / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004569:Electroencephalography / Q000379:methods; D008297:Male; D000077408:Modafinil; D016761:Oncogene Proteins v-fos / Q000378:metabolism; D045042:Pedunculopontine Tegmental Nucleus / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012894:Sleep Stages / Q000187:drug effects; D014851:Wakefulness / Q000187:drug effects*
D001559:Benzhydryl Compounds; D000697:Central Nervous System Stimulants; D016761:Oncogene Proteins v-fos; D000077408:Modafinil