title: 5-Fluorouracil-related gene expression in hepatic artery infusion-treated patients with hepatic metastases from colorectal carcinomas.
abstract: OBJECTIVE
  To predict the therapeutic efficacy of hepatic arterial infusion (HAI) with 5-fluorouracil (5FU) for patients with liver metastases from colorectal carcinomas, 5FU-related gene expressions were examined in primary colorectal carcinomas.
  
  
  METHODS
  Thirty-eight patients with liver metastases from colorectal carcinoma received HAI of 5FU. The expressions of the mRNAs for thymidine synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), and oroteta phophoribosyl transferase (OPRT) in primary colorectal carcinomas were measured by RT-PCR.
  
  
  RESULTS
  The response rate was 52.6% (20/38). The overall median survival time was 29.1 months. DPD and TP expression was significantly higher in the progressive disease (PD) group than in the complete response (CR) or partial response (PR) group (p = 0.032, p = 0.014), respectively. The levels of DPD and TP mRNAs showed a significant correlation (r = 0.76, p = 0.0001).
  
  
  CONCLUSIONS
  The expression of DPD and TP mRNAs in primary colorectal carcinomas was significantly predictive of the therapeutic response to 5FU HAI.
authors.affiliation: Department of Surgery, Gunma Cancer Center, Ota, Gunma, Japan. shinsame@gunma-cc.jp
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sameshima
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tomozawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horikoshi
authors.forename: Kenta
authors.initials: K
authors.lastname: Motegi
authors.forename: Isao
authors.initials: I
authors.lastname: Hirayama
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Koketsu
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Okada
authors.forename: Masaru
authors.initials: M
authors.lastname: Kojima
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kon
authors.forename: Toshio
authors.initials: T
authors.lastname: Sawada
mesh_terms: D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage*; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005472:Fluorouracil / Q000008:administration & dosage*; D015870:Gene Expression / Q000187:drug effects; D006499:Hepatic Artery; D006801:Humans; D007261:Infusions, Intra-Arterial; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics / Q000556:secondary; D008297:Male; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013939:Thymidine Phosphorylase / Q000096:biosynthesis / Q000235:genetics; D013940:Thymidylate Synthase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000964:Antimetabolites, Antineoplastic; D012333:RNA, Messenger; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase; D009962:Orotate Phosphoribosyltransferase; D013939:Thymidine Phosphorylase; D005472:Fluorouracil