Determination of analyte concentration using the residual solvent resonance in (1)H NMR spectroscopy.
An NMR protocol that uses the residual proton signal from DMSO -d(6) (i.e., DMSO -d(5)) to determine the concentration of an analyte in a NMR sample was developed. This technique provides an alternative method for determining the molar concentration of compounds in solution without prior knowledge of their molecular weight. The method is particularly useful when submilligram quantities of compound are to be analyzed and is applicable to a variety of different research areas such as compound management, and natural product, combinatorial, and medicinal chemistry.
Pierens
Gregory K
GK
Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, QLD, Australia. Greg.Pierens@cmr.uq.edu.au
Carroll
Anthony R
AR
Davis
Rohan A
RA
Palframan
Meredith E
ME
Quinn
Ronald J
RJ
D001688:Biological Products / Q000032:analysis*; D002110:Caffeine / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure
D001688:Biological Products; D002110:Caffeine; D004121:Dimethyl Sulfoxide