Effects of the mango components mangiferin and quercetin and the putative mangiferin metabolite norathyriol on the transactivation of peroxisome proliferator-activated receptor isoforms.
Mangos are a source of bioactive compounds with potential health-promoting activity. This study evaluated the abilities of the mango components quercetin and mangiferin and the aglycone derivative of mangiferin, norathyriol, to modulate the transactivation of peroxisome proliferator-activated receptor isoforms (PPARs). PPARs are transcription factors important in many human diseases. Through the use of a gene reporter assay it was shown that quercetin inhibited the activation of all three isoforms of PPARs (PPARgamma IC(50) = 56.3 microM; PPARalpha IC(50) = 59.6 microM; PPARbeta IC(50) = 76.9 microM) as did norathyriol (PPARgamma IC(50) = 153.5 microM; PPARalpha IC(50) = 92.8 microM; PPARbeta IC(50) = 102.4 microM), whereas mangiferin did not inhibit the transactivation of any isoform. These findings suggest that mango components and metabolites may alter transcription and could contribute to positive health benefits via this or similar mechanisms.
Wilkinson
Ashley S
AS
School of Pharmacy and Centre for Nutrition and Food Sciences, University of Queensland, Brisbane, QLD 4072, Australia.
Monteith
Gregory R
GR
Shaw
P Nicholas
PN
Lin
Chun-Nam
CN
Gidley
Michael J
MJ
Roberts-Thomson
Sarah J
SJ
D005638:Fruit / Q000737:chemistry; D006801:Humans; D031022:Mangifera / Q000737:chemistry*; D047492:Peroxisome Proliferator-Activated Receptors / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D011794:Quercetin / Q000494:pharmacology*; D015533:Transcriptional Activation / Q000187:drug effects*; D014162:Transfection; D014966:Xanthenes / Q000494:pharmacology*; D044004:Xanthones / Q000494:pharmacology*
D047492:Peroxisome Proliferator-Activated Receptors; D020033:Protein Isoforms; D014966:Xanthenes; D044004:Xanthones; C013592:mangiferin; C069053:norathyriol; D011794:Quercetin