Layer-by-layer assembly of viral capsid for cell adhesion.
Cowpea mosaic virus (CPMV)-based thin films are biologically active for cell culture. Using layer-by-layer assembly of CPMV and poly(diallyldimethylammonium chloride), quantitatively scalable biomolecular surfaces were constructed, which were well characterized using quartz crystal microbalance, UV-vis and atomic force microscopy. The surface coverage of CPMV nanoparticles depended on the adsorption time and pH of the virus solution, with a greater amount of CPMV adsorption occurring near its isoelectric point. It was found that the adhesion and proliferation of NIH-3T3 fibroblasts can be controlled by the coverage of viral particles using this multilayer technique.
Lin
Y
Y
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, China.
Su
Z
Z
Niu
Z
Z
Li
S
S
Kaur
G
G
Lee
L A
LA
Wang
Q
Q
D000327:Adsorption / Q000187:drug effects; D000818:Animals; D002213:Capsid / Q000378:metabolism* / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D017798:Comovirus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D051379:Mice; D018625:Microscopy, Atomic Force; D041681:NIH 3T3 Cells; D011095:Polyethylenes / Q000494:pharmacology; D011791:Quartz; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013997:Time Factors
D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; D011791:Quartz; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride