Pathogenicity of entomopathogenic fungi on hibernating pupae of Cameraria ohridella Deschka & Dimic 1986 (Lepidoptera, Gracillariidae). Part 1: Pathogenicity against the naked pupa.
Strains from Paecilomyces fumosoroseus, Lecanicillium muscarium, Metarhizium anisopliae and Beauveria bassiana were examined in standardized Biotest to control the horse-chestnut leaf miner (Cameraria ohridella) in her pupal stage in winter. The fungi were pathogenic against the hibernating pupae of Cameraria ohridella at dose of 1.9 x 10(7) conidia/ml. They were aggressive, led to infection, death and mouldiness of naked pupae. Even at low temperature of 5 degrees C and 12 degrees C. L. muscarium strain V24 showed the highest pathogenicity after 4 weeks against this host, close followed by P. fumosoroseus strain P6. M. anisopliae strain 72 and 8. bassiana strain B412 were also pathogen but after a long-time period. Experiments gave information for general susceptibility of naked pupae of C. ohridella under low temperatures against entomopathogenic fungi. In further examinations it has to be tested, whether fungi can infected, when the pupae stay in their natural surroundings, the pupal cell in the leaf.
Richter
Doreen
D
Humboldt University of Berlin, Institute of Horticultural Sciences, Department of Phytomedicine, Lentzeallee 55/57, DE-14195 Berlin, Germany.
Sermann
Helga
H
Jäckel
Barbara
B
Büttner
Carmen B
CB
D031319:Aesculus / Q000469:parasitology*; D000818:Animals; D001203:Ascomycota / Q000472:pathogenicity / Q000502:physiology; D052982:Beauveria / Q000472:pathogenicity / Q000502:physiology; D015169:Colony Count, Microbial; D007814:Larva; D007915:Lepidoptera / Q000254:growth & development* / Q000382:microbiology; D052981:Metarhizium / Q000472:pathogenicity / Q000502:physiology; D003904:Mitosporic Fungi / Q000472:pathogenicity / Q000502:physiology*; D010143:Paecilomyces / Q000472:pathogenicity / Q000502:physiology; D010572:Pest Control, Biological / Q000379:methods*; D011157:Population Dynamics; D012621:Seasons; D013696:Temperature