Expression of SV2 in the developing chick cerebellum: comparison with Calbindin and AMPA glutamate receptors 2/3.
The well-organized cerebellum is an ideal model to investigate the developmental appearance and localization of pre- and postsynaptic structures. One of the synaptic proteins abundant in the central nervous system and localized in presynaptic vesicle membranes is the synaptic vesicle protein 2 (SV2). SV2 was shown to be involved in priming and modulating synaptic vesicles and having an effect in epileptic diseases. So far there are no data available describing the developmental localization of this protein in the cerebellum. We followed the expression pattern of SV2 and compared it with the expression of the neuronal calcium-binding protein Calbindin and the AMPA glutamate receptor subunits 2/3 (GluR 2/3), both shown to be early expressed in the developing chick cerebellum predominantly in Purkinje cells. We detected the expression of SV2 in presynaptic terminals (mainly from climbing and mossy fibers) as soon as they are formed at embryonic day 16 in the inner molecular layer. Purkinje cells express Calbindin and GluR 2/3 in the soma and postsynaptically in the primary dendrites at this stage. With ongoing development, the pattern of SV2 expression follows the development of Purkinje cell dendrites in the molecular layer, suggesting a synaptic refinement of labeled climbing and later parallel fibers.
Grabs
Detlev
D
University Fribourg, Department of Medicine, Division of Anatomy, Fribourg, Switzerland. detlev.grabs@uqtr.ca
Escher
Lukas
L
Bergmann
Mathias
M
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D064026:Calbindins; D002531:Cerebellum / Q000254:growth & development* / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens; D017729:Presynaptic Terminals / Q000378:metabolism*; D018091:Receptors, AMPA / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D050599:Vesicular Glutamate Transport Protein 2 / Q000378:metabolism
D015415:Biomarkers; D064026:Calbindins; D018091:Receptors, AMPA; D064030:S100 Calcium Binding Protein G; D050599:Vesicular Glutamate Transport Protein 2; C104723:glutamate receptor ionotropic, AMPA 3; C104722:glutamate receptor ionotropic, AMPA 2