title: Dynamic expression of immune response genes in the sea bass, Dicentrarchus labrax, experimentally infected with the monogenean Diplectanum aequans.
abstract: The sea bass, Dicentrarchus labrax, is one of the most extensively farmed marine fishes in the Mediterranean. Under the high-density condition common in aquaculture, the monogenean gill parasite Diplectanum aequans can cause significant economic losses. This study used real-time quantitative PCR to investigate the dynamic expression of immune response genes in sea bass infected with Diplectanum aequans. The target genes, interleukin-1 (IL-1beta, transforming growth factor (TGF-beta and T-cell receptor (TCR-beta), were studied in the gills and spleen of the sea bass from the first day of infection until thirty days post- infection. Our results showed that there was an increase in IL-1beta gene expression in the spleen and gills and in TGF-beta gene expression in the gills of infected fish. These results show that parasitic infection induced a local inflammatory reaction and that reaction was restricted to the site of infection. Finally, the absence of relationship between TCR-beta expression and the parasitic infection suggests that the adaptive immune system is not involved in the response against this parasite.
authors.affiliation: Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5244, CNRS, EPHE, UPVD, Université de Perpignan, 58 avenue Paul Alduy, 66860 Perpignan Cedex, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Faliex
authors.forename: Catherine
authors.initials: C
authors.lastname: Da Silva
authors.forename: Gaël
authors.initials: G
authors.lastname: Simon
authors.forename: Pierre
authors.initials: P
authors.lastname: Sasal
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D049628:Body Size / Q000276:immunology; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D005786:Gene Expression Regulation; D019674:Genes, T-Cell Receptor beta / Q000235:genetics; D005880:Gills / Q000276:immunology; D053583:Interleukin-1beta / Q000235:genetics; D013154:Spleen / Q000276:immunology; D053773:Transforming Growth Factor beta1 / Q000235:genetics; D014200:Trematoda / Q000276:immunology*; D014201:Trematode Infections / Q000235:genetics / Q000469:parasitology / Q000662:veterinary*
chemical_list: D053583:Interleukin-1beta; D053773:Transforming Growth Factor beta1