Bioassembled nanocircuits of Mo6S9-xIx nanowires for electrochemical immunodetection of estrone Hapten.
We demonstrate a novel and highly sensitive electrochemical detection of estrone based on an immunosensor platform, composed of bioassembled nanocircuits of Mo 6S 9- x I x nanowires (MoSI NWs) covalently connected to anti-estrone antibodies. The one-step, label-free, and quantitative detection of estrone is realized by employing the [Ru(NH 3) 6] (3+/2+) redox ions to sense anti-estrone antibody and estrone interactions. The MoSI NWs/anti-estrone antibody nanocircuit architectures provide an amplification and conductive pathway for the specific electrochemical sensing of estrone hapten. A detection limit of 1.4 pg x mL (-1) was achieved in contrast to previous electrochemical techniques in which the sensitivity was limited to the nanomolar range.
Sun
Nijuan
N
College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, People's Republic of China.
McMullan
Martin
M
Papakonstantinou
Pagona
P
Gao
Hui
H
Zhang
Xinxiang
X
Mihailovic
Dragan
D
Li
Meixian
M
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry / Q000295:instrumentation*; D004970:Estrone / Q000032:analysis*; D006241:Haptens / Q000032:analysis*; D007454:Iodides / Q000737:chemistry*; D008982:Molybdenum / Q000737:chemistry*; D053770:Nanowires; D013056:Spectrophotometry, Ultraviolet; D013455:Sulfur / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D006241:Haptens; D007454:Iodides; D004970:Estrone; D013455:Sulfur; D008982:Molybdenum