title: Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad.
abstract: Although recent reports have described multipotent, self-renewing, neural crest-derived stem cells (NCSCs), the NCSCs in various adult rodent tissues have not been well characterized or compared. Here we identified NCSCs in the bone marrow (BM), dorsal root ganglia, and whisker pad and prospectively isolated them from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. Cultured EGFP-positive cells formed neurosphere-like structures that expressed NCSC genes and could differentiate into neurons, glial cells, and myofibroblasts, but the frequency of the cell types was tissue source dependent. Interestingly, we observed NCSCs in the aorta-gonad-mesonephros region, circulating blood, and liver at the embryonic stage, suggesting that NCSCs migrate through the bloodstream to the BM and providing an explanation for how neural cells are generated from the BM. The identification of NCSCs in accessible adult tissue provides a new potential source for autologous cell therapy after nerve injury or disease.
authors.affiliation: Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Narihito
authors.initials: N
authors.lastname: Nagoshi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Shibata
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kubota
authors.forename: Masaya
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Nagai
authors.forename: Etsuko
authors.initials: E
authors.lastname: Satoh
authors.forename: Satoru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Yohei
authors.initials: Y
authors.lastname: Okada
authors.forename: Yo
authors.initials: Y
authors.lastname: Mabuchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katoh
authors.forename: Seiji
authors.initials: S
authors.lastname: Okada
authors.forename: Keiichi
authors.initials: K
authors.lastname: Fukuda
authors.forename: Toshio
authors.initials: T
authors.lastname: Suda
authors.forename: Yumi
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D001853:Bone Marrow / Q000502:physiology*; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry; D005727:Ganglia, Spinal / Q000166:cytology* / Q000502:physiology; D020869:Gene Expression Profiling; D006066:Gonads / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007124:Immunoenzyme Techniques; D019426:Integrases / Q000378:metabolism; D008297:Male; D008650:Mesonephros / Q000166:cytology; D051379:Mice; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D009432:Neural Crest / Q000166:cytology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014738:Vibrissae / Q000166:cytology* / Q000502:physiology; D051155:Wnt1 Protein / Q000502:physiology
chemical_list: D012333:RNA, Messenger; D051155:Wnt1 Protein; C495246:Wnt1 protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases