title: SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis.
abstract: Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden. The expression profile was obtained from serial analysis of gene expression (SAGE) library data produced from 3- and 6-year-old trees. Fourteen-base expressed sequence tags (ESTs) were searched against public Eucalyptus ESTs and annotated with GenBank. Altogether 43,304 tags were generated producing 3066 unigenes with three or more copies each, 445 with a putative identity, 215 with unknown function and 2406 without an EST match. The expression profile of the juvenile cambial region revealed the presence of highly frequent transcripts related to general metabolism and energy metabolism, cellular processes, transport, structural components and information pathways. We made a quantitative analysis of a large number of genes involved in the biosynthesis of cellulose, pectin, hemicellulose and lignin. Our findings provide insight into the expression of functionally related genes involved in juvenile wood formation in young fast-growing E. grandis trees.
authors.affiliation: Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba-SP, Brazil.
authors.forename: Mayra Costa da Cruz
authors.initials: MC
authors.lastname: Gallo de Carvalho
authors.forename: Danielle Gregorio Gomes
authors.initials: DG
authors.lastname: Caldas
authors.forename: Raphael Tozelli
authors.initials: RT
authors.lastname: Carneiro
authors.forename: David Henry
authors.initials: DH
authors.lastname: Moon
authors.forename: Guillermo Rafael
authors.initials: GR
authors.lastname: Salvatierra
authors.forename: Lívia Maria
authors.initials: LM
authors.lastname: Franceschini
authors.forename: Alexander
authors.initials: A
authors.lastname: de Andrade
authors.forename: Paola Alejandra Fiorani
authors.initials: PA
authors.lastname: Celedon
authors.forename: Shinitiro
authors.initials: S
authors.lastname: Oda
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Labate
mesh_terms: D000064:Acclimatization; D003001:Cloning, Molecular; D017931:DNA Primers; D004798:Enzymes / Q000235:genetics; D005052:Eucalyptus / Q000235:genetics* / Q000254:growth & development; D020869:Gene Expression Profiling; D015723:Gene Library; D017343:Genes, Plant; D006019:Glycolysis; D010940:Plant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D004798:Enzymes; D010940:Plant Proteins