Using imaging and genetics in zebrafish to study developing spinal circuits in vivo.
Imaging and molecular approaches are perfectly suited to young, transparent zebrafish (Danio rerio), where they have allowed novel functional studies of neural circuits and their links to behavior. Here, we review cutting-edge optical and genetic techniques used to dissect neural circuits in vivo and discuss their application to future studies of developing spinal circuits using living zebrafish. We anticipate that these experiments will reveal general principles governing the assembly of neural circuits that control movements.
McLean
David L
DL
Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, USA. d.mclean@cornell.edu
Fetcho
Joseph R
JR
D000818:Animals; D003952:Diagnostic Imaging; D005821:Genetic Techniques; D009434:Neural Pathways / Q000502:physiology*; D013116:Spinal Cord / Q000033:anatomy & histology* / Q000254:growth & development*; D015027:Zebrafish