Che-1 enhances cyclin-dependent kinase 5 expression and interacts with the active kinase-complex.
Che-1 is a nuclear protein involved in the regulation of gene transcription and cell proliferation. It has also been shown to localize to the cytoplasm of postmitotic neuronal cells, where it is able to interact with the microtubule-associated protein tau. Cyclin-dependent kinase 5 (Cdk5) is a postmitotic proline-directed serine/threonine kinase that hyperphosphorylates tau under pathological conditions. We observed that Che-1 overexpression induces Cdk5 expression both at the mRNA and protein levels. Furthermore, we show that Che-1 directly interacts with Cdk5 protein in vivo. Cdk5/Che-1 complex formation does not compete with Cdk5/p35 interaction, thus Che-1 is able to bind the active kinase complex. Finally, we demonstrated that Che-1 is itself a Cdk5 substrate.
Buontempo
Serena
S
Istituto di Biologia e Patologia Molecolari, CNR, Rome, Italy.
Barbato
Christian
C
Bruno
Tiziana
T
Corbi
Nicoletta
N
Ciotti
Maria Teresa
MT
Floridi
Aristide
A
Fanciulli
Maurizio
M
Passananti
Claudio
C
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D051360:Cyclin-Dependent Kinase 5 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D051379:Mice; D009474:Neurons / Q000502:physiology*; D009687:Nuclear Proteins; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection / Q000379:methods
C523104:Aatf protein, rat; D009687:Nuclear Proteins; D014157:Transcription Factors; D051360:Cyclin-Dependent Kinase 5