Effect of cell culture on 18S rRNA gene sequences in the cultural course of Taxus chinensis cells.
Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.
Xiang
Fu
F
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. xiangfu@smail.hust.edu.cn
Yu
Long J
LJ
Chen
Wu
W
Liu
Zhi
Z
D002478:Cells, Cultured; D002681:China; D016384:Consensus Sequence; D008969:Molecular Sequence Data; D010802:Phylogeny; D018749:RNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D012337:RNA, Ribosomal, 18S / Q000235:genetics* / Q000302:isolation & purification; D020946:Taxus / Q000145:classification / Q000166:cytology / Q000235:genetics*
D018749:RNA, Plant; D012337:RNA, Ribosomal, 18S