title: Synergistic anti-tumor effect of combination radio- and immunotherapy by electro-gene therapy plus intra-tumor injection of dendritic cells.
abstract: Interleukin-2 (IL-2) and granulocyte-macrophage colony stimulating factor (GM-CSF) facilitate the maturation and functioning of injected DC. We developed a method of in situ electroporation using IL-2 and GM-CSF genes (EGT/cytokines), followed by intra-tumoral (i.t.) immature DC to determine the immune response at the tumor site using prostate-specific antigen-transfected CT26 cells. Three cycles of EGT/cytokines and i.t. DC inhibited tumor growth most effectively, but not superior to EGT/cytokines alone. However, the role of i.t. DC became significant when radiation was given after immunotherapy, which may have clinical implications on achieving better local control and prevention of systemic relapse.
authors.affiliation: Department of Radiation Therapy and Oncology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Wang
authors.forename: Yuk-Wah
authors.initials: YW
authors.lastname: Tsang
authors.forename: Chau-Hua
authors.initials: CH
authors.lastname: Chi
authors.forename: Chao-Chun
authors.initials: CC
authors.lastname: Chang
authors.forename: Rea-Min
authors.initials: RM
authors.lastname: Chu
authors.forename: Kwan-Hwa
authors.initials: KH
authors.lastname: Chi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D003713:Dendritic Cells / Q000637:transplantation*; D018274:Electroporation; D015316:Genetic Therapy / Q000379:methods*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D007267:Injections; D007376:Interleukin-2 / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D020360:Neoadjuvant Therapy; D009374:Neoplasms, Experimental / Q000276:immunology / Q000532:radiotherapy / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D007376:Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor