title: The effect of topical doxycycline in the prevention of experimental tympanosclerosis.
abstract: OBJECTIVE
  The aim of this study is to determine the effectiveness of topical doxycycline used in the process of experimental myringosclerosis and tympanosclerosis.
  
  
  METHODS
  A prospective experimental animal study.
  
  
  METHODS
  Experimental tympanosclerosis was accomplished in 25 healthy adult guinea pigs by inoculation with 2.5 x 10(7) colony-forming units of type-3 Streptococcus pneumoniae microorganisms followed by bilateral myringotomy. While the animals' right ears received a topical doxycycline treatment daily, their left ears were left untreated and used as controls. Otomicroscopic examination was carried out weekly and healing tympanic membranes were remyringotomized. After a 6-week follow-up, the temporal bones of 24 of 25 animals were removed and light-microscopy examination was done regarding tympanic membrane myringosclerosis and middle ear mucosal sclerosis.
  
  
  RESULTS
  Myringosclerosis was noticed to a lesser extent in the doxycycline-treated group when compared to the untreated control group. Light microscopy evaluation revealed a difference in the area and thickness of the sclerotic plaques of myringosclerosis of the tympanic membranes in the doxycycline-treated group and the control group, being significantly smaller and thinner in the treated group (P < .001, P < .04, respectively). Similarly, the area and thickness of the sclerotic plaques in the middle ear mucosa were significantly smaller and thinner in the doxycycline treated group (P < .001, P < .03).
  
  
  CONCLUSIONS
  This study demonstrated that the potent matrix metallo-proteinase inhibitor doxycycline plays a preventive role in the development of experimentally induced tympanosclerosis.
authors.affiliation: Fourth ENT Clinic, Ankara Numune Education and Research Hospital, Ankara, Turkey. ibrahimozcandr@gmail.com
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Ozcan
authors.forename: Adin
authors.initials: A
authors.lastname: Selcuk
authors.forename: K Murat
authors.initials: KM
authors.lastname: Ozcan
authors.forename: Ozgur
authors.initials: O
authors.lastname: Akdogan
authors.forename: Seren G
authors.initials: SG
authors.lastname: Giray
authors.forename: Huseyin
authors.initials: H
authors.lastname: Dere
authors.forename: Candan
authors.initials: C
authors.lastname: Ozogul
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004318:Doxycycline / Q000008:administration & dosage*; D006168:Guinea Pigs; D008297:Male; D045726:Metalloproteases / Q000037:antagonists & inhibitors; D011446:Prospective Studies; D012598:Sclerosis / Q000517:prevention & control; D014432:Tympanic Membrane / Q000473:pathology*
chemical_list: D000900:Anti-Bacterial Agents; D045726:Metalloproteases; D004318:Doxycycline