Contrast staining of Trichinella spiralis larvae in fresh, frozen and formalin preserved muscles.
Fresh, frozen-thawed and formalin-preserved muscle samples heavily infected with Trichinella larvae were cut into several pieces and stained with Giemsa and Leishman and the reference Haematoxylineosin (H & E) stain. Observation under microscope revealed that both muscle larvae and nurse cells in fresh and formalin preserved specimens appeared as purplish blue structures contrasting with the pinkish color of non-infected muscle fibers in both Giemsa and Leishman stains. These findings were confirmed in H & E stained samples. However, frozen samples did not show contrast stain.
Ali
Nehad M
NM
Department of Parasitology, Faculty of Medicine, Ain-Shams University, Cairo 11566, Egypt.
Thabet
Hala S
HS
D000818:Animals; D001399:Azure Stains; D005260:Female; D007814:Larva; D009132:Muscles / Q000469:parasitology*; D051381:Rats; D013048:Specimen Handling / Q000379:methods*; D017160:Trichinella spiralis / Q000302:isolation & purification* / Q000648:ultrastructure; D014235:Trichinellosis / Q000175:diagnosis / Q000469:parasitology
D001399:Azure Stains