Transposon-based strategies for the identification of essential bacterial genes.
We present a conceptual review of transposition-based strategies for determining gene essentiality on a one-by-one basis in bacteria. Many of the techniques are described in greater detail in individual chapters of this volume. The second section of this chapter deals with transposition-deletion-based strategies for determining the essentiality of blocks of genes. This latter approach has the potential to experimentally define the minimal required genome for a given organism.
Reznikoff
William S
WS
Marine Biological Laboratory, Bay Paul Center for Comparative Molecular Biology and Evolution, Woods Hole, MA, USA.
Winterberg
Kelly M
KM
D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D020043:Genes, Essential; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D016254:Mutagenesis, Insertional / Q000379:methods
D004251:DNA Transposable Elements; D004269:DNA, Bacterial