Extracellular transglutaminase 2 activates beta-catenin signaling in calcifying vascular smooth muscle cells.
Accumulation of transglutaminase 2 (TG2) is often associated with mineral deposits in vasculature. Here, we demonstrate that purified TG2 stimulated a 3-fold increase in matrix mineralization and up-regulation of osteoblastic markers in cultured primary vascular smooth muscle cells (VSMCs). Extracellular TG2 interacts with the low density lipoprotein related-protein 5 receptor and activates beta-catenin signaling in VSMCs. These results suggest that TG2 may promote vascular calcification by activating the beta-catenin signaling pathway.
Faverman
Lidia
L
Tufts University, Department of Anatomy and Cell Biology, 136 Harrison Avenue, Boston, MA 02111, United States.
Mikhaylova
Lyudmila
L
Malmquist
Jennifer
J
Nurminskaya
Maria
M
D000818:Animals; D002114:Calcinosis / Q000139:chemically induced* / Q000378:metabolism; D002478:Cells, Cultured; D019204:GTP-Binding Proteins / Q000494:pharmacology*; D006168:Guinea Pigs; D051379:Mice; D008810:Mice, Inbred C57BL; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D015398:Signal Transduction; D011503:Transglutaminases / Q000494:pharmacology*; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism*
D051153:Wnt Proteins; D051176:beta Catenin; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins