Purification and structural characterization of bacillomycin F produced by a bacterial honey isolate active against Byssochlamys fulva H25.
OBJECTIVE
Isolate and characterize antifungal peptides exhibiting activity against Byssochlamys fulva H25, a spoilage mould associated with juices and beverages.


RESULTS
A bacterium (H215) isolated from honey showed high antifungal activity against B. fulva H25. The antifungal producer strain was identified as Bacillus subtilis using 16S rDNA sequencing. The antifungal peptide was purified by 20% ammonium sulfate precipitation of the bacterial culture supernatant, followed by Octyl-Sepharose CL-4B and reverse phase-high performance liquid chromatography. The five active fractions were lyophilized and subjected to mass, tandem mass spectrometry and amino acid analysis to deduce their corresponding molecular masses and structural characteristics. The five peaks were determined to be identical to bacillomycin F, varying in the length of the fatty acid chain moiety from C14 to C16.


CONCLUSIONS
The broad-spectrum antifungal activity produced by a bacterium from honey was determined to be due to the production of bacillomycin F.


CONCLUSIONS
The antifungal compound produced by a bacterial strain isolated from honey was determined to be stable over a broad pH range and was stable to heat treatments up to 100 degrees C. This is the first report of honey microflora producing bacillomycin F or any antifungal compound.
Lee
H
H
Department of Food Science and Technology, New York State Agricultural Experiment Station, Cornell University, NY, USA.
Churey
J J
JJ
Worobo
R W
RW
D000596:Amino Acids / Q000032:analysis; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification*; D001412:Bacillus subtilis / Q000378:metabolism*; D001628:Beverages / Q000382:microbiology; D055323:Byssochlamys; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005516:Food Microbiology; D006722:Honey / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods; D008826:Microbial Sensitivity Tests; D010456:Peptides, Cyclic / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D021521:Ribotyping / Q000379:methods
D000596:Amino Acids; D000935:Antifungal Agents; D010456:Peptides, Cyclic; C034571:bacillomycin F