Protective effect of lipoic acid against methylglyoxal-induced oxidative stress in LLC-PK(1) cells.
Methylglyoxal (MG), a reactive dicarbonyl compound, is a metabolic byproduct of glycolysis often found at high levels in blood from diabetic patients. The effect of lipoic acid on MG-induced oxidative stress was investigated using LLC-PK(1) renal tubular epithelial cells, which are susceptible to oxidative stress. MG (500 microM) treatment induced LLC-PK(1) cell death to nearly 50% compared with non-treated control cells, but lipoic acid significantly inhibited the MG-induced cytotoxicity in a concentration-dependent manner. In addition, lipoic acid treatment dose-dependently reduced the intracellular reactive oxygen species level increased by 500 microM MG. The nitric oxide level was also increased by 500 microM MG treatment, but it was significantly inhibited by lipoic acid. Furthermore, lipoic acid treatment at 50 microM inhibited the nuclear translocation of nuclear factor-kappa B induced by MG treatment in LLC-PK(1) cells. These findings indicate that lipoic acid has potential as a therapeutic agent against the development of diabetic complications related to MG-induced oxidative stress in diabetes.
Kim
Hyun Young
HY
Institute of Natural Medicine, University of Toyama, Toyama, Japan.
Oi
Yasuyuki
Y
Kim
Mujo
M
Yokozawa
Takako
T
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology; D018374:LLC-PK1 Cells; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011765:Pyruvaldehyde / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000737:chemistry / Q000378:metabolism; D013552:Swine; D008063:Thioctic Acid / Q000494:pharmacology*
D000975:Antioxidants; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D011765:Pyruvaldehyde; D008063:Thioctic Acid