title: The influence of acetylsalicylic acid on oxylipin migration in Cryptococcus neoformans var. neoformans UOFS Y-1378.
abstract: In this paper we report the influence of acetylsalicylic acid on oxylipin migration in Cryptococcus neoformans var. neoformans UOFS Y-1378, previously isolated from human bone lesion. Transmission electron microscopy suggests that osmiophilic material originates in mitochondria and is deposited inside the yeast cell wall, from which it is excreted into the environment, along capsule protuberances, or through capsule detachments. Previous studies using immunogold labeling indicate that these osmiophilic layers contain 3-hydroxy oxylipins. In this study, the addition of acetylsalicylic acid (an inhibitor of mitochondrial function) in increasing amounts to the cells abrogated the migration of osmiophilic material, as well as capsule detachment from cell walls, and hence, oxylipin excretion. Consequently, we hypothesize that 3-hydroxy oxylipins are produced in mitochondria, probably via incomplete beta-oxidation or fatty acid synthesis, from which they are deposited inside the cell wall and excreted through tubular protuberances attached to the surrounding capsules and (or) through detachment of these oxylipin-containing capsules.
authors.affiliation: Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, P.O. Box 339, Nelson Mandela Drive, Bloemfontein, Free State 9301, South Africa.
authors.forename: Olihile M
authors.initials: OM
authors.lastname: Sebolai
authors.forename: Carolina H
authors.initials: CH
authors.lastname: Pohl
authors.forename: Piet J
authors.initials: PJ
authors.lastname: Botes
authors.forename: Pieter W J
authors.initials: PW
authors.lastname: van Wyk
authors.forename: Johan L F
authors.initials: JL
authors.lastname: Kock
mesh_terms: D001241:Aspirin / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D003455:Cryptococcus neoformans / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000737:chemistry / Q000648:ultrastructure; D008954:Models, Biological; D054883:Oxylipins / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D054883:Oxylipins; D001241:Aspirin