Microterricola viridarii gen. nov., sp. nov., a new member of the family Microbacteriaceae.
Strain KV-677T, a Gram-positive, aerobic, motile, rod-shaped bacterium, was isolated from park soil in Tokyo, Japan, and characterized. It grew well at 15-30 degrees C on nutrient agar and colonies were pale yellow. The cell-wall peptidoglycan contained diaminobutyric acid, glycine, alanine and glutamic acid and the muramic acid acyl type was acetyl. The predominant menaquinone was MK-12. Mycolic acids were not detected. The DNA G+C content was 70 mol%. 16S rRNA gene sequence analysis revealed that strain KV-677T fell within the cluster of the family Microbacteriaceae and formed a separate lineage joining the genera Salinibacterium, Rhodoglobus, Subtercola and Agreia, showing 95.5-96.9 % sequence similarities with the type strains of the type species of the above genera. However, strain KV-677T clearly differed from these and other genera with relatively high sequence similarity in its chemotaxonomic characteristics. Therefore, it is proposed that strain KV-677T represents a novel species in a new genus, Microterricola viridarii gen. nov., sp. nov., in the family Microbacteriaceae. The type strain of Microterricola viridarii is KV-677T (=NRRL B-24538T =NBRC 102123T).
Matsumoto
Atsuko
A
The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Yamada
Mayuko
M
Omura
Satoshi
S
Takahashi
Yōko
Y
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D007564:Japan; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity; D024482:Vitamin K 2 / Q000378:metabolism
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2