Glycodelin expression in the endometrium of healthy women and in the eutopic and ectopic endometrium of women with endometriosis.
OBJECTIVE
To analyze the expression of the glycodelin gene to better understand the molecular environment of endometriotic lesions and to elucidate the potential mechanisms that underlie the complex physiopathology of endometriosis.


METHODS
Prospective laboratory study.


METHODS
University hospital.


METHODS
Eleven healthy fertile women and 17 patients with endometriosis in the early proliferative phase of the menstrual cycle.


METHODS
Endometrial biopsy specimens were obtained from the endometrium of healthy women without endometriosis (controls) and from eutopic and ectopic endometrium tissues (pelvic and ovarian endometriotic implants) of endometriosis patients.


METHODS
The glycodelin relative expression level by real-time polymerase chain reaction (PCR) analysis.


RESULTS
The glycodelin down-regulation found in the endometriotic lesions was 332.26 and 123.17-fold lower, respectively, when compared with the eutopic tissue and the control endometrium.


CONCLUSIONS
Glycodelin may be one of the molecules that contributes to the loss of cellular homeostasis in endometriotic lesions.
Meola
Juliana
J
Department of Genetics, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. jumeola@yahoo.com.br
Dentillo
Daniel Blassioli
DB
Rosa e Silva
Júlio César
JC
Ferriani
Rui Alberto
RA
Veiga
Luciana Caricati
LC
Paro de Paz
Cláudia Cristina
CC
Giuliatti
Silvana
S
Martelli
Lúcia
L
D000293:Adolescent; D000328:Adult; D002828:Choristoma / Q000378:metabolism*; D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D000071816:Glycodelin; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D011257:Pregnancy Proteins / Q000235:genetics*; D011446:Prospective Studies
D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins