Differentiation of Sclerotinia minor depends on thiol redox state and oxidative stress.
Sclerotial differentiation in Sclerotinia minor is associated with oxidative stress and thiol redox state. The significance of oxidative stress to sclerotial differentiation was revealed by the higher oxidative stress of S. minor compared with a nonsclerotiogenic counterpart. The effect of thiol redox state on sclerotial differentiation was shown by the antioxidant action of the thiol (-SH) group of N-acetylcysteine and cysteine and by an unknown (not antioxidant) role of glutathione (GSH) on S. minor. The nonantioxidant role of GSH was indicated by the differentiation-inhibiting and differentiation-noninhibiting actions of the GSH biosynthesis inhibitor L-buthionine-S,R-sulfoximine and the GSH biosynthesis inducer L-2-oxo-thiazolidine-4-carboxylate, respectively, and by the increase of oxidative stress they caused during the transition from the undifferentiated to differentiated state of S. minor. Moreover, N-acetylcysteine can be used as a potent nontoxic fungicide against this phytopathogenic fungus by acting as a growth-inhibiting cytotoxic oxidant and by sustaining the fungus in the undifferentiated hyphal stage, which is vulnerable to degradation by soil microorganisms.
Patsoukis
Nikolaos
N
Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, 26100-Patras, Greece.
Georgiou
Christos D
CD
D000111:Acetylcysteine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011761:Pyrrolidonecarboxylic Acid; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D053778:Thiazolidines
D000975:Antioxidants; D006131:Growth Inhibitors; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D053778:Thiazolidines; D005978:Glutathione; D011761:Pyrrolidonecarboxylic Acid; D000111:Acetylcysteine; C029536:2-oxothiazolidine-4-carboxylic acid