title: Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone.
abstract: Benzalacetone synthase (BAS) from Rheum palmatum is a plant-specific type III polyketide synthase that catalyzes the one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group P2(1), with unit-cell parameters a = 54.6, b = 89.6, c = 81.1 A, alpha = gamma = 90.0, beta = 100.5 degrees . Diffraction data were collected to 1.8 A resolution using synchrotron radiation at BL24XU of SPring-8.
authors.affiliation: Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Morita
authors.forename: Michikazu
authors.initials: M
authors.lastname: Tanio
authors.forename: Shin
authors.initials: S
authors.lastname: Kondo
authors.forename: Ryohei
authors.initials: R
authors.lastname: Kato
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Wanibuchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Sugio
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Kohno
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000378:metabolism; D002074:Butanones / Q000737:chemistry / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D002074:Butanones; D010940:Plant Proteins; D011994:Recombinant Proteins; C025383:benzylideneacetone; D000217:Acyltransferases; C020141:flavanone synthetase