title: Decidual natural killer cell tuning by autologous dendritic cells.
abstract: OBJECTIVE
  Dendritic cells (DC)/natural killer (NK) cells interactions in the deciduas of early human pregnancies were analyzed in vitro.
  
  
  METHODS
  Phenotype, cytokine expression and/or cytolytic mediators' expression were measured by flow cytometry in NK and DC from the freshly isolated decidual mononuclear cells or after their purification and co-culture in vitro. Proliferation of 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE)-labeled CD56(+) cells was analyzed by flow cytometry after the co-culture with CD1a(+) or CD83(+) DC.
  
  
  RESULTS
  Decidual CD1a(+) cells show less mature phenotype with no expression of CD197, lower expression of CD80 and CD86 and higher expression of CD206 and CD195 in comparison to CD83(+) cells. Interleukin (IL)-15, interferon-gamma and tumor necrosis factor-alpha productions were higher in immature than mature DC, whereas IL-10 and IL-18 were equally produced in both subpopulations. Immature DC increase perforin, FasL and TRAIL protein expression and proliferation of NK cells, but decrease their intracellular IL-15 production. Mature DC caused less efficient proliferation of NK cells, and did not affect cytokine and cytolytic mediator expression.
  
  
  CONCLUSIONS
  These results suggest that decidual CD1a(+) cells regulate and shape NK cell function more profoundly than CD83(+) cells in decidua.
authors.affiliation: Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia. gordana.laskarin@medri.hr
authors.forename: Gordana
authors.initials: G
authors.lastname: Laskarin
authors.forename: Arnela
authors.initials: A
authors.lastname: Redzović
authors.forename: Zeljka
authors.initials: Z
authors.lastname: Rubesa
authors.forename: Alberto
authors.initials: A
authors.lastname: Mantovani
authors.forename: Paola
authors.initials: P
authors.lastname: Allavena
authors.forename: Herman
authors.initials: H
authors.lastname: Haller
authors.forename: Ivan
authors.initials: I
authors.lastname: Vlastelić
authors.forename: Daniel
authors.initials: D
authors.lastname: Rukavina
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D018949:Antigens, CD1 / Q000032:analysis; D019002:CD56 Antigen / Q000032:analysis; D002450:Cell Communication / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D003656:Decidua / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D016130:Immunophenotyping / Q000379:methods; D007694:Killer Cells, Natural / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis; D010641:Phenotype; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D019002:CD56 Antigen; D016207:Cytokines; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen; C419393:CD1a antigen