title: Carbonyl cyanide m-chlorophenylhydrazone induced calcium signaling and activation of plasma membrane H(+)-ATPase in the yeast Saccharomyces cerevisiae.
abstract: The plasma membrane H(+)-ATPase from Saccharomyces cerevisiae is an enzyme that plays a very important role in the yeast physiology. The addition of protonophores, such as 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), also triggers a clear in vivo activation of this enzyme. Here, we demonstrate that CCCP-induced activation of the plasma membrane H(+)-ATPase shares some similarities with the sugar-induced activation of the enzyme. Phospholipase C and protein kinase C activities are essential for this activation process while Gpa2p, a G protein involved in the glucose-induced activation of the ATPase, is not required. CCCP also induces a phospholipase C-dependent increase in intracellular calcium. Moreover, we show that the availability of extracellular calcium is required for CCCP stimulation of H(+)-ATPase, suggesting a possible connection between calcium signaling and activation of ATPase.
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus do Morro do Cruzeiro, Ouro Preto, MG - Brazil.
authors.forename: Michele B P
authors.initials: MB
authors.lastname: Pereira
authors.forename: Renata
authors.initials: R
authors.lastname: Tisi
authors.forename: Luciano G
authors.initials: LG
authors.lastname: Fietto
authors.forename: Anamaria S
authors.initials: AS
authors.lastname: Cardoso
authors.forename: Mônica M
authors.initials: MM
authors.lastname: França
authors.forename: Fernanda M
authors.initials: FM
authors.lastname: Carvalho
authors.forename: Maria José M
authors.initials: MJ
authors.lastname: Trópia
authors.forename: Enzo
authors.initials: E
authors.lastname: Martegani
authors.forename: Ieso M
authors.initials: IM
authors.lastname: Castro
authors.forename: Rogelio L
authors.initials: RL
authors.lastname: Brandão
mesh_terms: D002118:Calcium / Q000032:analysis; D020013:Calcium Signaling / Q000187:drug effects*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000031:analogs & derivatives* / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology*; D003600:Cytosol / Q000737:chemistry; D044385:GTP-Binding Protein alpha Subunits / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D044385:GTP-Binding Protein alpha Subunits; D007476:Ionophores; D029701:Saccharomyces cerevisiae Proteins; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D011493:Protein Kinase C; D010738:Type C Phospholipases; D006180:Proton-Translocating ATPases; C083907:Gpa2 protein, S cerevisiae; D002118:Calcium