Effect of using RNA interference to alter iNOS gene expression on the proliferation of tongue squamous cell carcinoma cell line Tca8113.
This study used RNA interference (RNAi) to explore the effect of NO and inducible nitric oxide synthase (iNOS) on apoptosis and proliferation in the tongue squamous carcinoma cell line Tca8113. Tca8113 cells were transfected with the plasmid pGenesil-1, which expresses iNOS short hairpin RNA (shRNA), or the negative control plasmid pSilencer-HK, and the transfected cells were compared with untransfected cells. The expression of iNOS was detected by histochemistry, and apoptosis was detected by flow cytometry. The expression of iNOS was significantly lower in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. The apoptosis rate was significantly higher in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. Growth monitoring showed that proliferation was also inhibited in cells transfected with pSilencer-iNOS. RNAi gene silencing decreased iNOS gene expression, induced apoptosis, and suppressed proliferation in Tca8113 cells.
Chen
Wei-liang
WL
Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, PR China. drchen@vip.136.com
Yang
Lan
L
Zeng
Shu-guang
SG
Wang
Yong-jie
YJ
D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004396:Coloring Agents; D005434:Flow Cytometry; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020868:Gene Silencing / Q000502:physiology; D006651:Histocytochemistry; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D009569:Nitric Oxide / Q000235:genetics; D052247:Nitric Oxide Synthase Type II / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D034622:RNA Interference / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D013778:Tetrazolium Salts; D013844:Thiazoles; D014062:Tongue Neoplasms / Q000235:genetics / Q000473:pathology; D014162:Transfection
D004396:Coloring Agents; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; D013844:Thiazoles; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; C022616:thiazolyl blue