title: Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater.
abstract: A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33-51 degrees C (optimum 44-46 degrees C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 omega 10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T).
authors.affiliation: Institute of Environmental Engineering and Biotechnology, Tampere University of Technology, Tampere, Finland.
authors.forename: Anna H
authors.initials: AH
authors.lastname: Kaksonen
authors.forename: Stefan
authors.initials: S
authors.lastname: Spring
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Reiner M
authors.initials: RM
authors.lastname: Kroppenstedt
authors.forename: Jaakko A
authors.initials: JA
authors.lastname: Puhakka
mesh_terms: D001482:Base Composition; D019149:Bioreactors; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D045859:Desulfotomaculum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D005798:Genes, Bacterial; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D013171:Spores, Bacterial / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism; D009626:Terminology as Topic; D014866:Waste Products; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008670:Metals; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013431:Sulfates; D014866:Waste Products