Anti-inflammatory action of alpha-melanocyte stimulating hormone (alpha-MSH) in anti-CD3/CD28-mediated spleen and CD4(+)CD25(-) T cells and a partial participation of IL-10.
alpha-Melanocyte stimulating hormone (alpha-MSH) has been shown to inhibit the production and the effects of pro-inflammatory cytokine by inflammatory cells in innate immunity. We have determined whether alpha-MSH inhibits anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells proliferation and its mechanism of action. The proliferation of anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells markedly were suppressed by 50-100 nM and 5-100 nM alpha-MSH, respectively. alpha-MSH (100 nM) increased the production of the anti-inflammatory cytokine IL-10 and decreased the production of the pro-inflammatory cytokine IL-2 and IFN-gamma from CD4(+)CD25(-) T cells. Moreover, anti-IL-10 blocking Ab decreased the inhibitory effects of anti-CD3/CD28-mediated spleen cells and CD4(+)CD25(-) T cells proliferation by alpha-MSH, indicating a partial participation of IL-10 in its mechanism of inhibitory action. These results suggest that alpha-MSH may be useful for treatment of autoimmune diseases and transplantation involving innate and adaptive immunity.
Chang
Sung Ho
SH
Department of Surgery and Asan Institute for Life Sciences, Ulsan University College of Medicine & Asan Medical Center, Songpa-Gu, Seoul, Republic of Korea.
Jung
Eun Jung
EJ
Lim
Dong Gyun
DG
Park
Youn Hee
YH
Wee
Yu Mee
YM
Kim
Jin Hee
JH
Kim
Yang Hee
YH
Choi
Monica Young
MY
Koo
Sun Kyung
SK
Choi
Kang Duk
KD
Han
Duck Jong
DJ
Kim
Song Cheol
SC
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000187:drug effects* / Q000276:immunology*; D000521:alpha-MSH / Q000494:pharmacology*
D015703:Antigens, CD; D018106:CD28 Antigens; D017252:CD3 Complex; D007376:Interleukin-2; D016753:Interleukin-10; D000521:alpha-MSH; D017493:Leukocyte Common Antigens