Repopulation of donor cells from the recipient following extremity graft: studies using the LACZ transgenic rat.
Cell traffic from the recipient into the graft, so called graft chimerism, appears to play a major role in graft acceptance. This study was conducted using LacZ transgenic rats to define recipient cell migration into whole-limb grafts. Eighteen hind limb transplants were performed across LacZ+ transgenic and LacZ- rats. LacZ expression was evaluated by X-gal staining. Intense LacZ expression was observed at the interfollicular epidermis in the skin component of LacZ- limb grafts 48 weeks after transplant. Muscle fibers and cartilage showed intense LacZ expression in LacZ+ limb grafts at 48 weeks but no expression in LacZ- grafts. Polymerase chain reaction studies confirmed the presence of the LacZ gene in the bone and skin of LacZ- limb grafts and in the muscle of LacZ+ grafts. Our results demonstrate that there is a gradual increase in repopulation of recipient cells in grafted skin and bone tissues, but muscle and cartilage tissues retain donor cell origin.
Muramatsu
Keiichi
K
Department of Orthopedic Surgery, Yamaguchi University School of Medicine, Yamaguchi, Japan. muramatu@yamaguchi-u.ac.jp
Kuriyama
Ryutaro
R
Taguchi
Toshihiko
T
D000818:Animals; D030801:Animals, Genetically Modified; D002465:Cell Movement / Q000235:genetics*; D046528:Chimerism; D002863:Chromogenic Compounds; D005697:Galactosides; D006614:Hindlimb / Q000503:physiopathology / Q000637:transplantation*; D007211:Indoles; D007763:Lac Operon; D008297:Male; D016133:Polymerase Chain Reaction; D051381:Rats; D018183:Transplantation Chimera / Q000235:genetics*; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000235:genetics
D002863:Chromogenic Compounds; D005697:Galactosides; D007211:Indoles; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside