title: Carbon and energy balances of glucose fermentation with hydrogenproducing bacterium Citrobacter amalonaticus Y19.
abstract: For the newly isolated H2-producing chemoheterotrophic bacterium Citrobacter amalonaticus Y19, anaerobic glucose metabolism was studied in batch cultivation at varying initial glucose concentrations (3.5- 9.5 g/l). The carbon-mass and energy balances were determined and utilized to analyze the carbon metabolic-pathways network. The analyses revealed (a) variable production of major metabolites (H2, ethanol, acetate, lactate, CO2, and cell mass) depending on initial glucose levels; (b) influence of NADH regeneration on the production of acetate, lactate, and ethanol; and (c) influence of the molar production of ATP on the production of biomass. The results reported in this paper suggest how the carbon metabolic pathway(s) should be designed for optimal H2 production, especially at high glucose concentrations, such as by blocking the carbon flux via lactate dehydrogenase from the pyruvate node.
authors.affiliation: Bioenergy Research Center, Korea Institute of Energy Research, Daejeon 305-543, Korea.
authors.forename: You-Kwan
authors.initials: YK
authors.lastname: Oh
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Seol
authors.forename: Seo Hyoung
authors.initials: SH
authors.lastname: Kim
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Jae-Woong
authors.initials: JW
authors.lastname: Hwang
authors.forename: Dewey D Y
authors.initials: DD
authors.lastname: Ryu
mesh_terms: D000693:Anaerobiosis; D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D002954:Citrobacter / Q000302:isolation & purification / Q000378:metabolism*; D004734:Energy Metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology
chemical_list: D012722:Sewage; D002244:Carbon; D006859:Hydrogen; D005947:Glucose