Nuclear swelling occurs during premature senescence mediated by MAP kinases in normal human fibroblasts.
Excess thymidine induced premature senescence in normal human fibroblasts (TIG-7), with induction of typical senescence markers. Nuclear swelling, as well as cell swelling, was clearly observed in these senescent cells. Simultaneous addition of MAP kinase inhibitors, U0126, SB203580, and SP60025, effectively suppressed induction of premature senescence and senescence markers.
Kobayashi
Yusuke
Y
Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, 641-12 Maioka-cho, Yokohama 244-0813, Japan.
Sakemura
Risa
R
Kumagai
Atsuko
A
Sumikawa
Emi
E
Fujii
Michihiko
M
Ayusawa
Dai
D
D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D053145:Cell Nucleus Size; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016922:Cellular Senescence; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013936:Thymidine / Q000494:pharmacology
D015415:Biomarkers; D020928:Mitogen-Activated Protein Kinases; D013936:Thymidine