title: Loss of methylation imprint of Snrpn in postovulatory aging mouse oocyte.
abstract: Prolonged residence of postovulatory oocyte in the oviduct or prolonged culture in vitro can lead to oocyte aging, which significantly affects pre- and post-implantation embryo development. In this study, we employed bisulfite sequencing and COBRA methods to investigate the DNA methylation status of differentially methylated regions (DMRs) of Snrpn and Peg1/Mest, two maternally imprinted genes, in postovulatory oocytes aged in vivo and in vitro. The results showed that Snrpn DMR was clearly demethylated in oocytes aged in vivo at 29h post-hCG and in denuded oocytes aged in vitro for the same time period. However, Peg1/Mest did not show any demethylation in all aged groups at 29h post-hCG. These data indicate that oocytes undergo time-dependent demethylation of Snrpn DMR during the process of postovulatory aging.
authors.affiliation: Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, China.
authors.forename: Xing-Wei
authors.initials: XW
authors.lastname: Liang
authors.forename: Jia-Qiao
authors.initials: JQ
authors.lastname: Zhu
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Miao
authors.forename: Jing-He
authors.initials: JH
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Wei
authors.forename: Sheng-Sheng
authors.initials: SS
authors.lastname: Lu
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Ke-Huan
authors.initials: KH
authors.lastname: Lu
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D016922:Cellular Senescence / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D018392:Genomic Imprinting; D051379:Mice; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D011506:Proteins / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013447:Sulfites / Q000737:chemistry; D055517:snRNP Core Proteins
chemical_list: D001324:Autoantigens; D011506:Proteins; D017411:Ribonucleoproteins, Small Nuclear; D013447:Sulfites; C103637:mesoderm specific transcript protein; D055517:snRNP Core Proteins; D004247:DNA; C042345:hydrogen sulfite