title: Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview.
abstract: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
authors.affiliation: University of Natural Resources and Applied Life Sciences Vienna, Department of Biotechnology, Vienna, Austria. diethard.mattanovich@boku.ac.at.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Gasser
authors.forename: Markku
authors.initials: M
authors.lastname: Saloheimo
authors.forename: Ursula
authors.initials: U
authors.lastname: Rinas
authors.forename: Martin
authors.initials: M
authors.lastname: Dragosits
authors.forename: Escarlata
authors.initials: E
authors.lastname: Rodríguez-Carmona
authors.forename: Kristin
authors.initials: K
authors.lastname: Baumann
authors.forename: Maria
authors.initials: M
authors.lastname: Giuliani
authors.forename: Ermenegilda
authors.initials: E
authors.lastname: Parrilli
authors.forename: Paola
authors.initials: P
authors.lastname: Branduardi
authors.forename: Christine
authors.initials: C
authors.lastname: Lang
authors.forename: Danilo
authors.initials: D
authors.lastname: Porro
authors.forename: Pau
authors.initials: P
authors.lastname: Ferrer
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Tutino
authors.forename: Diethard
authors.initials: D
authors.lastname: Mattanovich
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde