A novel pyrene-guanidiniocarbonyl-pyrrole cation efficiently differentiates between ds-DNA and ds-RNA by two independent, sensitive spectroscopic methods.
At micromolar concentrations and equimolar conditions in respect to basepairs, a novel pyrene-guanidiniocarbonyl-pyrrole cation 1 exhibited a strong ICD signal at about lambda=300nm specifically upon the interaction with ds-DNA, while under the same conditions a new fluorescence maximum at lambda=480nm appeared exclusively upon the addition of ds-RNA.
Hernandez-Folgado
Laura
L
Institut für Organische Chemie, Universität Würzburg, Am Hubland, 97074, Würzburg, Germany.
Schmuck
Carsten
C
Tomić
Sanja
S
Piantanida
Ivo
I
D002412:Cations / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D019791:Guanidine / Q000031:analogs & derivatives*; D007364:Intercalating Agents / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D011721:Pyrenes / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D002412:Cations; D005456:Fluorescent Dyes; D007364:Intercalating Agents; D011721:Pyrenes; D011758:Pyrroles; D012330:RNA, Double-Stranded; D004247:DNA; D019791:Guanidine