title: A novel murine model of allogeneic vaccination against renal cancer.
abstract: OBJECTIVE
  To develop a murine model for whole-cell allogeneic vaccination in renal cancer, as such vaccines aim to direct immune responses against patient tumour cells, due to shared antigens between the vaccine and tumour cells.
  
  
  METHODS
  A novel murine renal cell line, allogeneic to BALB/c, was developed from a C57BL/6 mouse by primary cell culture (RVIK). It was immortalized by HPV16 E6/E7 and transfected with ras in an attempt to improve its immunogenicity. The cell line was characterized and tested as a vaccine in a BALB/c tumour-protection model after subsequent tumour challenge with autologous RenCa tumour cells.
  
  
  RESULTS
  RVIK alone, with no ras induced cross-reactive immunity, providing a valid non-tumorigenic allogeneic whole-cell vaccine model for renal cancer. Ras transfection per se did not improve RVIK immunity.
  
  
  CONCLUSIONS
  RVIK is a novel immunogenic murine renal epithelial cell line, which confers protection when used as an allogeneic vaccine. It provides proof of principle for the effectiveness of allogeneic whole-cell vaccines and may therefore form the basis of a useful model of allogeneic vaccination to further optimize vaccination schedules, formulation and adjuvants for a clinical setting.
authors.affiliation: Department of Urology, St. George's Hospital, London, UK.
authors.forename: Erik G
authors.initials: EG
authors.lastname: Havranek
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Labarthe
authors.forename: Stephen
authors.initials: S
authors.lastname: Ward
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Anderson
authors.forename: Michael A
authors.initials: MA
authors.lastname: Whelan
authors.forename: Hardev
authors.initials: H
authors.lastname: Pandha
mesh_terms: D000818:Animals; D019496:Cancer Vaccines; D002292:Carcinoma, Renal Cell / Q000473:pathology / Q000517:prevention & control*; D002455:Cell Division; D007680:Kidney Neoplasms / Q000473:pathology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D016019:Survival Analysis; D014407:Tumor Cells, Cultured
chemical_list: D019496:Cancer Vaccines