Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP(+)-dependent xylitol dehydrogenase, and xylulokinase.
We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.
Matsushika
Akinori
A
Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. a-matsushika@aist.go.jp
Watanabe
Seiya
S
Kodaki
Tsutomu
T
Makino
Keisuke
K
Sawayama
Shigeki
S
D050542:D-Xylulose Reductase / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
D011994:Recombinant Proteins; D000431:Ethanol; D009249:NADP; D014994:Xylose; D050542:D-Xylulose Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D014993:Xylitol