title: Rainbow smelt (Osmerus mordax) genomic library and EST resources.
abstract: Genomic resources in rainbow smelt (Osmerus mordax) enable us to examine the genome duplication process in salmonids and test hypotheses relating to the fate of duplicated genes. They further enable us to pursue physiological and ecological studies in smelt. A bacterial artificial chromosome library containing 52,410 clones with an average insert size of 146 kb was constructed. This library represents an 11-fold average coverage of the rainbow smelt (O. mordax) genome. In addition, several complementary deoxyribonucleic acid libraries were constructed, and 36,758 sequences were obtained and combined into 12,159 transcripts. Over half of these transcripts have been identified, several of which have been associated with cold adaptation. These basic resources show high levels of similarity (86%) to salmonid genes and provide initial support for genome duplication in the salmonid ancestor. They also facilitate identification of genes important to fish and direct us toward new technologies for other studies in fish biology.
authors.affiliation: Centre for Biomedical Research, University of Victoria, Victoria, BC V8W 3N5, Canada.
authors.forename: K R
authors.initials: KR
authors.lastname: von Schalburg
authors.forename: J
authors.initials: J
authors.lastname: Leong
authors.forename: G A
authors.initials: GA
authors.lastname: Cooper
authors.forename: A
authors.initials: A
authors.lastname: Robb
authors.forename: M R
authors.initials: MR
authors.lastname: Beetz-Sargent
authors.forename: R
authors.initials: R
authors.lastname: Lieph
authors.forename: R A
authors.initials: RA
authors.lastname: Holt
authors.forename: R
authors.initials: R
authors.lastname: Moore
authors.forename: K V
authors.initials: KV
authors.lastname: Ewart
authors.forename: W R
authors.initials: WR
authors.lastname: Driedzic
authors.forename: B F H
authors.initials: BF
authors.lastname: ten Hallers
authors.forename: B
authors.initials: B
authors.lastname: Zhu
authors.forename: P J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: W S
authors.initials: WS
authors.lastname: Davidson
authors.forename: B F
authors.initials: BF
authors.lastname: Koop
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003080:Cold Temperature; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D029941:Fish Proteins / Q000235:genetics; D015723:Gene Library; D015698:Genomic Library; D008969:Molecular Sequence Data; D036441:Osmeriformes / Q000235:genetics*
chemical_list: D029941:Fish Proteins