title: [Effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism].
abstract: OBJECTIVE
  To investigate the effects of serum from crush injury rats on vascular endothelial cell apoptosis and their potential mechanism.
  
  
  METHODS
  Bovine aorta endothelial cells were cultured in vitro and the effects of serum from crush injury rats on cell apoptosis and intracellular calcium concentration ([Ca2+]i) were observed. Meanwhile, the levels of rat blood plasma endothelin-1 (ET-1) and atrial natriuretic peptide(ANP) were measured.
  
  
  RESULTS
  Compared with normal rat serum treatment, the cell apoptosis rate decreased from (8.26+/-1.75)% to (2.75+/-0.90)%, while the concentration of [Ca2+]i increased from (96.98+/-3.95) to (118.79+/-3.22) nmol/L in serum from crush injury rats, respectively. The concentration of ET-1 and ANP increased significantly in crush injury rat serum.
  
  
  CONCLUSIONS
  Serum from crush injury rats could inhibit apoptosis of the vascular endothelial cells. These effects may be related to increased level of [Ca2+]i mediated by ET-1 and ANP.
authors.affiliation: Department of Forensic Pathology, Zhongshan Medical School of Sun Yat-sen University, Guangzhou, China. liushp@mail.sysu.edu.cn
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Luo
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D009320:Atrial Natriuretic Factor / Q000097:blood*; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism*; D019332:Endothelin-1 / Q000097:blood*; D005121:Extremities / Q000293:injuries*; D005434:Flow Cytometry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003470:Culture Media; D019332:Endothelin-1; D009320:Atrial Natriuretic Factor; D002118:Calcium