title: Antimicrobial properties and elution kinetics of linezolid-loaded hip spacers in vitro.
abstract: Bacterial adhesion to and -persistence on antibiotic-loaded bone cement is an increasing problem. New antibiotics with good antimicrobial and pharmacokinetic properties (e.g. linezolid) may be the solution to this problem; however, few data concerning linezolid-loaded acrylic cement are currently available. Ten gentamicin-linezolid-loaded hip spacers (1 g gentamicin/2.4 g linezolid/80 g PMMA; five spacers including a metallic endoskeleton, five with no metallic components) were tested in vitro against a strain of methicillin-resistant Staphylococcus aureus with regard to antibiotic release and bacteria growth inhibition. Daily, the antibiotic elution was determined by high liquid performance chromatography (linezolid) and fluorescence polarization immunoassay (gentamicin), the bacteria growth inhibition photometrically at 546 nm. All spacers demonstrated growth inhibition for 8 days. Peak average concentrations were reached for both agents on day 1 (gentamicin-35.10 mug/mL [24.10-52.52], linezolid-36.28 mug/mL [22.87-71.76]). After 8 days, 0.97% [0.93-1.05%] of the initial amount of linezolid and 3.13% [2.85-3.31%] of gentamicin were meanly released from spacers containing a metallic endoskeleton. In those containing of simple cement these values were 1.22% [0.91-1.59%] and 2.67% [2.12-2.73%], respectively. Linezolid demonstrated acceptable elution kinetics from bone cement; however, further experimental research and animal studies should clarify any possible side effect of linezolid-loaded cement media before definitive use in the clinical practice.
authors.affiliation: Klinik für Orthopädie und Orthopädische Chirurgie, Universitätsklinikum des Saarlandes, Homburg/Saar, Germany. konstantinos.anagnostakos@uniklinik-saarland.de
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Anagnostakos
authors.forename: Jens
authors.initials: J
authors.lastname: Kelm
authors.forename: Sandra
authors.initials: S
authors.lastname: Grün
authors.forename: Eduard
authors.initials: E
authors.lastname: Schmitt
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Jung
authors.forename: Stefanie
authors.initials: S
authors.lastname: Swoboda
mesh_terms: D000081:Acetamides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects / Q000379:methods*; D001843:Bone Cements; D005839:Gentamicins / Q000008:administration & dosage / Q000493:pharmacokinetics; D006622:Hip Prosthesis / Q000382:microbiology*; D007700:Kinetics; D000069349:Linezolid; D008422:Materials Testing; D023303:Oxazolidinones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000081:Acetamides; D000890:Anti-Infective Agents; D001843:Bone Cements; D005839:Gentamicins; D023303:Oxazolidinones; D000069349:Linezolid