title: Protein 600 is a microtubule/endoplasmic reticulum-associated protein in CNS neurons.
abstract: There is an increasing body of literature pointing to cytoskeletal proteins as spatial organizers and interactors of organelles. In this study, we identified protein 600 (p600) as a novel microtubule-associated protein (MAP) developmentally regulated in neurons. p600 exhibits the unique feature to interact with the endoplasmic reticulum (ER). Silencing of p600 by RNA interference (RNAi) destabilizes neuronal processes in young primary neurons undergoing neurite extension and containing scarce staining of the ER marker Bip. Furthermore, in utero electroporation of p600 RNAi alters neuronal migration, a process that depends on synergistic actions of microtubule dynamics and ER functions. p600-depleted migrating neurons display thin, crooked, and "zigzag" leading process with very few ER membranes. Thus, p600 constitutes the only known MAP to associate with the ER in neurons, and this interaction may impact on multiple cellular processes ranging from neuronal development to neuronal maturation and plasticity.
authors.affiliation: Hotchkiss Brain Institute, Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Shim
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Asada
authors.forename: Gernot
authors.initials: G
authors.lastname: Neumayer
authors.forename: Hong Chi
authors.initials: HC
authors.lastname: Tran
authors.forename: Kei-ichiro
authors.initials: K
authors.lastname: Ishiguro
authors.forename: Kamon
authors.initials: K
authors.lastname: Sanada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nakatani
authors.forename: Minh Dang
authors.initials: MD
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002148:Calmodulin-Binding Proteins; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016253:Microscopy, Immunoelectron / Q000379:methods; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D016900:Neurofilament Proteins / Q000172:deficiency; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure*; D034622:RNA Interference / Q000502:physiology; D014162:Transfection / Q000379:methods; D014404:Tubulin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: D002148:Calmodulin-Binding Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D014404:Tubulin; C462941:beta3 tubulin, mouse; C044882:neurofilament protein L; D049452:Green Fluorescent Proteins; D044767:Ubiquitin-Protein Ligases; C528904:Ubr4 protein, mouse