In vitro replication of varicella-zoster virus in human retinal pigment epithelial cells.
Here we describe for the first time the productive in vitro infection of human retinal pigment epithelial cells by varicella-zoster virus (VZV), resulting in a typical cytopathic effect (CPE) that is characterized by enlarged cells with increased granularity. Depending on the CPE dissemination, high titers of up to 1.6 x 10(6) PFU of cell-free and cryostable VZV/ml can be recovered.
Schmidt-Chanasit
Jonas
J
Institute of Medical Virology, Hospital of Johann Wolfgang Goethe University, D-60596 Frankfurt am Main, Germany. H.W.Doerr@em.uni-frankfurt.de
Bleymehl
Karoline
K
Rabenau
Holger F
HF
Ulrich
Rainer G
RG
Cinatl
Jindrich
J
Doerr
Hans Wilhelm
HW
D000328:Adult; D002478:Cells, Cultured; D015925:Cryopreservation; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000821:virology; D014645:Herpesvirus 3, Human / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000821:virology*; D012160:Retina / Q000166:cytology / Q000821:virology*; D014779:Virus Replication