title: Microvascular injury, thrombosis, inflammation, and apoptosis in the pathogenesis of heatstroke: a study in baboon model.
abstract: OBJECTIVE
  Severe heatstroke is a leading cause of morbidity and mortality during heat waves. The pathogenesis of tissue injury, organ failure, and death in heatstroke is not well understood.
  
  
  RESULTS
  We investigated the pathways of heatstroke-induced tissue injury and cell death in anesthetized baboons (Papio hamadyras) subjected to environmental heat stress until core temperature attained 42.5 degrees C (moderate heatstroke; n = 3) or onset of severe heatstroke (n = 4) signaled by a fall in systolic blood pressure to < 90 mm Hg and rise in core temperature to 43.1+/-0.1 degrees C. Three sham-heated animals served as controls. Light and electron microscopy revealed widespread hemorrhage and thrombosis, transmural migration of leukocytes, and microvascular endothelium injury in severe heatstroke. Immunohistology and ultrastructural analysis demonstrated increased staining of endothelial von Willebrand factor (vWF), tissue factor (TF), and endothelial leukocyte-platelet interaction. Extensive apoptosis was noted in spleen, gut, and lung, and in hematopoeitic cells populating these organs. Double-labeling studies colocalized active caspase-3 and TF with apoptotic cells. Findings in sham-heated animals were unremarkable.
  
  
  CONCLUSIONS
  These data suggested that microvascular injury, thrombosis, inflammation, and apoptosis may play an important role in the pathogenesis of heatstroke injury.
authors.affiliation: Department of Pathology & Laboratory Medicine, King Faisal Specialist Hospital & Research Center, Riyadh 11211, Saudi Arabia.
authors.forename: George T
authors.initials: GT
authors.lastname: Roberts
authors.forename: Hazem
authors.initials: H
authors.lastname: Ghebeh
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Chishti
authors.forename: Falah
authors.initials: F
authors.lastname: Al-Mohanna
authors.forename: Rafaat
authors.initials: R
authors.lastname: El-Sayed
authors.forename: Futwan
authors.initials: F
authors.lastname: Al-Mohanna
authors.forename: Abderrezak
authors.initials: A
authors.lastname: Bouchama
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology; D001831:Body Temperature / Q000502:physiology; D004195:Disease Models, Animal; D004211:Disseminated Intravascular Coagulation; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology*; D018882:Heat Stress Disorders / Q000503:physiopathology; D018883:Heat Stroke / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D007249:Inflammation / Q000150:complications*; D048529:Papio hamadryas; D013925:Thromboplastin / Q000378:metabolism; D013927:Thrombosis / Q000150:complications*; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D014841:von Willebrand Factor; D013925:Thromboplastin