Sesame oil attenuates hepatic lipid peroxidation by inhibiting nitric oxide and superoxide anion generation in septic rats.
BACKGROUND
Sepsis is a major cause of mortality in the intensive care unit. Oxidative stress plays an important role in the pathogenesis of organ failure during sepsis. Sesame oil decreases circulating oxygen free radicals in septic rats; however, its effect on hepatic oxidative status is unknown. The authors examined the effect of sesame oil on hepatic lipid peroxidation in septic rats.


METHODS
Hepatic injury was induced using cecal ligation and puncture (CLP). Rats were divided into 4 groups: sham, rats given a sham operation without CLP; SO, rats given sesame oil alone; CLP, rats given saline and then CLP; and CS, rats given sesame oil and then CLP. All rats were first given a 1-week daily oral supplement of sesame oil or saline (4 mL/kg/d) and then CLP or a sham operation. The authors assessed hepatic oxidative stress by determining hepatic lipid peroxidation, hydroxyl radical, superoxide anion, and nitric oxide levels 12 hours after CLP. They also assessed xanthine oxidase activity and nitric oxide synthase expression.


RESULTS
Hepatic lipid peroxidation (P < .0001), hydroxyl radical (P < .05), superoxide anion (P < .05), and nitrite (P < .05) levels were significantly lower in sesame oil-treated septic rats. Furthermore, sesame oil significantly reduced xanthine oxidase activity (P < .01) and inducible nitric oxide synthase expression (P < .005) in septic rats.


CONCLUSIONS
Sesame oil might attenuate hepatic lipid peroxidation by inhibiting superoxide anion and nitric oxide, at least partially, in experimental septic rats.
Hsu
Dur-Zong
DZ
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan.
Chien
Se-Ping
SP
Li
Ya-Hui
YH
Chuang
Yin-Ching
YC
Chang
Yu-Chung
YC
Liu
Ming-Yie
MY
D000704:Analysis of Variance; D000818:Animals; D002432:Cecum / Q000293:injuries / Q000473:pathology / Q000601:surgery; D017665:Hydroxyl Radical; D008026:Ligation; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology / Q000293:injuries / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D018805:Sepsis / Q000517:prevention & control; D012715:Sesame Oil / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D013481:Superoxides / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000187:drug effects / Q000378:metabolism
D009573:Nitrites; D017382:Reactive Oxygen Species; D013481:Superoxides; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D012715:Sesame Oil; D052247:Nitric Oxide Synthase Type II; D014969:Xanthine Oxidase