title: A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase.
abstract: To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
authors.affiliation: Department of Life Science, Catholic University of Korea, Bucheon, Gyunggi 420-743, Korea.
authors.forename: Yeong-Deok
authors.initials: YD
authors.lastname: Kwon
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Pil
authors.initials: P
authors.lastname: Kim
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D015870:Gene Expression; D020869:Gene Expression Profiling; D005943:Gluconeogenesis / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000235:genetics* / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D000255:Adenosine Triphosphate; D010730:Phosphoenolpyruvate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP)