A multichannel electrophoresis microchip platform for rapid chiral selector screening.
This study presents a four-channel electrophoresis chip platform, featuring double-cross hydrostatic sample injection, for rapid chiral selector screening. This platform needs only five electrodes to drive microchip electrophoresis in four separate channels for screening four chiral selectors at a time. To demonstrate the performance of this screening platform, eight neutral CDs and their derivatives as chiral selectors were screened towards two FITC-labeled chiral compounds. The screening could be accomplished in less than 2 min. Dimethyl-beta-CD and hydroxypropyl-alpha-CD was demonstrated to be the appropriate selectors for FITC-norfenefrine and FITC-baclofen, respectively. The established platform is easy to operate and suitable for rapid screening process, which is expected to be a potential platform for high-throughput screening of chiral selectors.
Gao
Yan
Y
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China.
Luo
Yong
Y
Qin
Jianhua
J
Lin
Bingcheng
B
D001418:Baclofen / Q000737:chemistry*; D002021:Buffers; D046209:Electrophoresis, Microchip / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D009655:Octopamine / Q000031:analogs & derivatives* / Q000737:chemistry; D013237:Stereoisomerism; D047391:alpha-Cyclodextrins / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D002021:Buffers; D005456:Fluorescent Dyes; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; D009655:Octopamine; C005310:norfenefrine; D001418:Baclofen; D016650:Fluorescein-5-isothiocyanate