[Expression of annexin I in human pancreatic cancer and the influence of its down-regulation on biology of this cancer].
OBJECTIVE
To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer.


METHODS
A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test.


RESULTS
When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred.


CONCLUSIONS
Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.
Liu
Qian
Q
Department of General Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
Wang
Cheng-Feng
CF
Zhou
Zhuan
Z
Hu
Hai
H
Zhao
Dong-Bing
DB
Ni
Xiao-Guang
XG
Bai
Xiao-Feng
XF
Gao
Ji-Dong
JD
Tian
Yan-Tao
YT
Zhao
Ping
P
D017305:Annexin A1 / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D006801:Humans; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D017305:Annexin A1; D012333:RNA, Messenger; D034741:RNA, Small Interfering