title: The type III secretion system-dependent repression of NF-kappaB activation to the intracellular growth of Edwardsiella tarda in human epithelial cells.
abstract: Edwardsiella tarda is a pathogen with a broad host range infecting animals and humans. We have reported recently that the type III secretion system (TTSS) is essential for intracellular replication of the bacterium in murine macrophages. The present study shows that the TTSS is also needed for intracellular growth of the bacterium in human epithelial cells (HEp-2). However, different from the previous microarray analyses on murine macrophages, upregulation of the mRNA expression level of NF-kappaB target genes was not detected in the infected HEp-2 cells. The wild-type E. tarda, but not its TTSS mutant, actually repressed the tumor necrosis factor alpha-dependent NF-kappaB activation in an NF-kappaB reporter gene assay. These results suggest TTSS-dependent repression of the NF-kappaB activation in HEp-2 cells infected with E. tarda.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan. okudaj@mb.kyoto-phu.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Okuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Kiriyama
authors.forename: Eisuke
authors.initials: E
authors.lastname: Yamanoi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakai
mesh_terms: D045744:Cell Line, Tumor; D020609:Edwardsiella tarda / Q000254:growth & development*; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology*; D020869:Gene Expression Profiling; D017930:Genes, Reporter; D006801:Humans; D050296:Microbial Viability; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D037521:Virulence Factors