title: Distribution of dipeptidyl peptidase IV in patients with chronic tonsillitis.
abstract: In the pathogeneses of recurrent tonsillitis (RT) and tonsillar hypertrophy (TH), different immunological mechanisms are involved. Dipeptidyl peptidase IV (DPP IV) and aminopeptidase N (APN) participate in the regulation of the immune response during inflammation. In this study, the localization of DPP IV and the enzymatic activities of DPP IV and APN in 32 patients, 13 with RT and 19 with TH, who underwent tonsillectomy were investigated. The localization of DPP IV in tonsils was studied using histochemical and immunohistochemical methods. The enzymatic activities of DPP IV and APN in tonsillar lymphocytes and the patients' sera were determined kinetically at 37 degrees C using Gly-Pro-p-nitroanilide (for DPP IV) and Ala-p-nitroanilide (for APN) as chromogenic substrates. In samples from both RT and TH patients, DPP IV was found to localize mainly in extrafollicular areas of tonsillar tissue in a pattern corresponding to the T-cell distribution. Significantly higher (P < 0.001) levels of DPP IV and APN activities in sera from patients with TH than in sera from patients with RT were found. A correlation of DPP IV activities in sera and tonsillar lymphocytes from patients with TH was also found (r = 0.518; P < 0.05). Moreover, the results show that DPP IV and APN activities in sera decreased significantly with age. Tonsillar lymphocytes demonstrated a wide range of DPP IV and APN activities, without significant differences between the investigated groups. The results of this study show that the localization of DPP IV does not depend on the type of tonsillitis, whereas the variety in levels of DPP IV and APN activities in sera of patients with TH and RT suggests different patterns of participation of antigen-stimulated tonsils in the immune system.
authors.affiliation: Clinic for Othorhinolaryngology, Clinical Center Nis, Nis, Serbia. milan.orl@bankerinter.net
authors.forename: Milan
authors.initials: M
authors.lastname: Stankovic
authors.forename: Predrag
authors.initials: P
authors.lastname: Vlahovic
authors.forename: Verica
authors.initials: V
authors.lastname: Avramovic
authors.forename: Miroljub
authors.initials: M
authors.lastname: Todorovic
mesh_terms: D000293:Adolescent; D000328:Adult; D018826:CD13 Antigens / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D018819:Dipeptidyl Peptidase 4 / Q000097:blood*; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000201:enzymology; D008875:Middle Aged; D014068:Tonsillectomy; D014069:Tonsillitis / Q000201:enzymology* / Q000276:immunology* / Q000473:pathology
chemical_list: D018826:CD13 Antigens; D018819:Dipeptidyl Peptidase 4