Low-molecular-weight hyaluronan permeates through human intestinal Caco-2 cell monolayers via the paracellular pathway.
The intestinal permeability of low-molecular-weight hyaluronan (LMW-HA) was investigated by using cultured monolayers of Caco-2 cells. The amount of LMW-HA that permeated the Caco-2 monolayers was measured by a carbazole assay. The permeability of LMW-HA increased inversely with the molecular size and was dose-dependent. The transport was observed to be energy-independent, and was correlated with the tight junction (TJ) permeability. These results suggest that LMW-HA permeated the Caco-2 cell monolayers via the paracellular pathway.
Hisada
Nozomi
N
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Satsu
Hideo
H
Mori
Akira
A
Totsuka
Mamoru
M
Kamei
Jun-ichi
J
Nozawa
Takashi
T
Shimizu
Makoto
M
D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism*; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D010539:Permeability
D009844:Oligosaccharides; D006820:Hyaluronic Acid