title: Thermal desorption characterisation of molecularly imprinted polymers. Part II: Use of direct probe GC-MS analysis to study crosslinking effects.
abstract: A powerful method utilising direct probe thermal desorption GC-MS is presented for the study of molecularly imprinted polymers (MIPs). A series of 2-aminopyridine (2-apy)-imprinted methacrylic acid-ethyleneglycol dimethacrylate (MAA-EGDMA) copolymers were prepared under identical conditions but with varying amounts of EGDMA (crosslinking monomer). The use of appropriate temperature programmes permitted template removal, and the subsequent assessment of polymer affinity and specificity, all of which were found to be dependent on polymer composition and morphology. The system was sufficiently sensitive to identify a specific response of imprinted polymers over nonimprinted counterparts. Correlations were found to exist between thermal desorption analysis and solution phase binding, which was assessed by UV spectroscopy, where specificity was found to diminish with decreasing EGDMA concentration. This was attributed to the increased number of free carboxyl groups in those polymers containing a lower percentage of EGDMA. Thermal desorption profiles obtained for the analyte were found to be unaffected by the physical and chemical properties of the solvent used for analyte reloading.
authors.affiliation: Separation Science Research Group, Department of Chemical and Life Sciences, Waterford Institute of Technology, Cork Road, Waterford, Ireland. nholland@wit.ie
authors.forename: Niamh
authors.initials: N
authors.lastname: Holland
authors.forename: Patrick
authors.initials: P
authors.lastname: Duggan
authors.forename: Eleanor
authors.initials: E
authors.lastname: Owens
authors.forename: Wayne
authors.initials: W
authors.lastname: Cummins
authors.forename: June
authors.initials: J
authors.lastname: Frisby
authors.forename: Helen
authors.initials: H
authors.lastname: Hughes
authors.forename: Peter
authors.initials: P
authors.lastname: McLoughlin
mesh_terms: D003432:Cross-Linking Reagents / Q000032:analysis* / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D054802:Molecular Imprinting / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D011108:Polymers / Q000032:analysis* / Q000737:chemistry*; D016062:Porosity; D012680:Sensitivity and Specificity; D012996:Solutions; D012997:Solvents; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D011108:Polymers; D012996:Solutions; D012997:Solvents; D009584:Nitrogen