Capillary electrophoresis with laser-induced fluorescence: environmental applications.
Capillary electrophoresis (CE), especially free-zone CE, offers a relatively simple separation with moderate selectivity based on the mobility of ions in solution. Laser-induced fluorescence (LIF) detection, an extremely sensitive technique, can be coupled with a variety of separation conditions to achieve sensitive and quantitative results. When these techniques are combined, CE/LIF provides the sensitivity and increased selectivity that makes trace level environmental analysis of fluorescent compounds possible at or below levels typical for gas chromatography (GC)/mass spectrometry (MS). We offer a panoramic review of the role of these tools in solving environmental and related analytical problems before providing a detailed experimental protocol.
Riddick
Lee
L
National Exposure Research Laboratory, Environmental Sciences Division, US Environmental Protection Agency, Office of Research and Development, Las Vegas, NV, USA.
Brumley
William C
WC
D019075:Electrophoresis, Capillary / Q000379:methods*; D004777:Environment; D004784:Environmental Monitoring / Q000379:methods*; D005452:Fluoresceins; D005453:Fluorescence; D007834:Lasers; D052616:Solid Phase Extraction; D013048:Specimen Handling
D005452:Fluoresceins