title: Effect of a novel ascorbic derivative, disodium isostearyl 2-O-L-ascorbyl phosphate, on normal human dermal fibroblasts against reactive oxygen species.
abstract: The novel amphiphilic vitamin C derivative disodium isostearyl 2-O-L-ascorbyl phosphate (VCP-IS-2Na), which has a C(18) alkyl chain attached to the stable ascorbate derivative sodium L-ascorbic acid 2-phosphate (VCP-Na), was evaluated for reduction of cell damage induced by oxidative stress, ultraviolet A (UVA), ultraviolet B (UVB), and H(2)O(2); stimulation of collagen synthesis against UVA irradiation; and inhibition of matrix metalloproteinase-1 (MMP-1) activity induced by UVA in human normal dermal fibroblasts. VCP-IS-2Na pretreatment resulted in significant protection against cell damage induced by UVB, UVA, and H(2)O(2). The amount of type I collagen following UVA irradiation was increased by treatment with VCP-IS-2Na in a concentration-dependent manner. These effects of VCP-IS-2Na were superior to those of L-ascorbic acid (vitamin C, VC) and VCP-Na. On the other hand, VCP-IS-2Na suppressed 65% of the excess MMP-1 irradiated UVA, and VC and VCP-Na slightly suppressed it.
authors.affiliation: Department of Dermatorogy, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, JapanUEB37453@nifty.com.
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Shibayama
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Hisama
authors.forename: Sanae
authors.initials: S
authors.lastname: Matsuda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawase
authors.forename: Mamitaro
authors.initials: M
authors.lastname: Ohtsuki
authors.forename: Katsumi
authors.initials: K
authors.lastname: Hanada
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iwaki
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; C528521:disodium isostearyl 2-O-L-ascorbyl phosphate; D003094:Collagen; D020781:Matrix Metalloproteinase 1; D001205:Ascorbic Acid