Effects of polyamines and polyamine biosynthetic inhibitors on mitotic activity of Allium cepa root tips.
The genotoxic and cytotoxic effects of exogenous polyamines (PAs), putrescine (Put), spermidine (Spd), spermine (Spm) and PA biosynthetic inhibitors, alpha-difluoromethylornithine (DFMO), cyclohexilamine (CHA), methylglioxal bis-(guanylhydrazone) (MGBG) were investigated in the root meristems of Allium cepa L. The reduction of mitotic index and the induction of chromosomal aberrations such as bridges, stickiness, c-mitotic anaphases, micronuclei, endoredupliction by PAs and PA biosynthetic inhibitors were observed and these were used as evidence of genotoxicity and cytotoxicity.
Unal
Meral
M
Department of Biology, Arts and Sciences Faculty, Marmara University, Goztepe 81040, Istanbul, Turkey.
Palavan-Unsal
Narcin
N
Tufekci
M A
MA
D003513:Cycloheximide / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018519:Meristem / Q000166:cytology / Q000187:drug effects*; D008935:Mitoguazone / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects* / Q000502:physiology; D019697:Onions / Q000187:drug effects* / Q000502:physiology; D011073:Polyamines / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D011073:Polyamines; D011500:Protein Synthesis Inhibitors; D013096:Spermine; D003513:Cycloheximide; D008935:Mitoguazone; D013095:Spermidine; D011700:Putrescine; D000518:Eflornithine