IL-6 regulates MCP-1, ICAM-1 and IL-6 expression in human myoblasts.
The inflammatory reaction in autoimmune polymyositis and rejection of transplanted myoblasts is characterized by mononuclear cell infiltration. In other settings monocytes are locally recruited by an IL-6-induced IL-8-to-MCP-1 switch. IL-6, upon binding to soluble gp80 (sIL-6R), can interact with membrane-bound ubiquitously expressed gp130 and activate virtually all cells (transsignaling). We found that human myoblasts could use transsignaling to produce IL-6, MCP-1 and ICAM-1; the addition of sIL-6R, binding to IL-1beta-induced IL-6, greatly increases IL-6 production. These in vitro data support the hypothesis that locally secreted IL-6 can target monocyte chemotaxis and leukocytes trafficking through an IL-6, MCP-1 and ICAM-1 modulation.
Marino
Mariapaola
M
Institute of General Pathology, Università Cattolica, L.go F. Vito 1, 00168 Roma, Italy.
Scuderi
Flavia
F
Provenzano
Carlo
C
Scheller
Jürgen
J
Rose-John
Stefan
S
Bartoccioni
Emanuela
E
D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism* / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000166:cytology; D032446:Myoblasts / Q000187:drug effects*; D013997:Time Factors
C111835:CCL2 protein, human; D018932:Chemokine CCL2; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1