title: Sampling and analytical methodology development for the determination of primary and secondary low molecular weight amines in ambient air.
abstract: In this project, a sampling device and an analytical method have been developed to simultaneously analyse the most frequently found low molecular weight amines, including aliphatic, aromatic and alcohol amines. These amines are diethanolamine, ethanolamine, methylamine, isopropylamine, morpholine, dimethylamine, and aniline. A sampling device was developed using a 37 mm cassette with glass fibre filters impregnated with sulfuric acid. Immediately after sampling, the filter was transferred to vials containing a solution of dansyl chloride. Dansyl chloride was used for derivatisation because it forms aromatic sulfonamides that are fluorescent and easy to protonate for MS detection. The effect of using an internal standard made with the dansylated derivative of 1-(2-methoxyphenyl)piperazine (MOPIP) on the uncertainty and efficiency of the method was also evaluated. This internal standard was spiked directly onto filters. The coupling of HPLC/ESI-MS was used for the simultaneous analysis of all the derivatives. This method showed detection limits of about 0.03 microg mL(-1) to 0.3 microg mL(-1) of amine with an average expanded uncertainty of 3% to 6% depending on the amine. The methodology recoveries are close to 100% for all the amines, and the overall estimated expanded uncertainties vary between 10% and 13% depending on the amine. This new strategy will be useful in evaluating workplace air since a unique sampling system will be used, independent of the amine to be quantified.
authors.affiliation: Institut de Recherche Robert-Sauvé en Santé et en Sécurité du Travail, 505 De Maisonneuve Blvd. West, Montréal, H3A 3C2, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Fournier
authors.forename: Jacques
authors.initials: J
authors.lastname: Lesage
authors.forename: Claude
authors.initials: C
authors.lastname: Ostiguy
authors.forename: Huu
authors.initials: H
authors.lastname: Van Tra
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis*; D000588:Amines / Q000032:analysis*; D000814:Aniline Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004123:Dimethylamines / Q000032:analysis; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D019856:Ethanolamine / Q000032:analysis; D004983:Ethanolamines / Q000032:analysis; D008744:Methylamines / Q000032:analysis; D008970:Molecular Weight; D009025:Morpholines / Q000032:analysis; D016273:Occupational Exposure / Q000032:analysis*; D011437:Propylamines / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D000395:Air Pollutants, Occupational; D000588:Amines; D000814:Aniline Compounds; D004123:Dimethylamines; D004983:Ethanolamines; D008744:Methylamines; D009025:Morpholines; D011437:Propylamines; D019856:Ethanolamine; C037574:morpholine; C034516:dimethylamine; C020283:diethanolamine; C027451:methylamine; C035263:2-propylamine; C023650:aniline