title: Oral administration of a hop water extract ameliorates the development of dermatitis induced by the periodical topical application of a mite antigen in atopic dermatitis model NC/Nga mice.
abstract: We investigated the inhibitory effect of an oral administration of a hop water extract (HWE) on the development of dermatitis by using NC/Nga atopic dermatitis model mice. The induction of allergic dermatitis was conducted by tape-stripping and topical application of a mite antigen (Dermatophagoides farinae) on to the ear once a week for 10 weeks. HWE was orally administered at a dose of 100 or 500 mg/kg. The total immunoglobulin E (IgE) concentration in serum and the ear thickness were periodically examined. Finally, the antigen-specific IgE level in the serum and the production of interleukin (IL)-4, IL-12 and interferon (IFN)-gamma from splenocytes and cervical lymph node cells were measured. The oral administration of HWE significantly inhibited the increase of total IgE production and ear swelling throughout the experimental period. The production of IL-12 was significantly lower in the HWE administered group than in the control group. The results suggest that the intake of HWE may be effective in preventing and alleviating the development of atopic dermatitis-like skin disease.
authors.affiliation: Frontier Laboratories of Value Creation, Sapporo Breweries Ltd, 10 Okatohme, Yaizu, Shizuoka 425-0013, Japan. syuuichi.segawa@sapporobeer.co.jp
authors.forename: Shuichi
authors.initials: S
authors.lastname: Segawa
authors.forename: Hisao
authors.initials: H
authors.lastname: Kuroda
authors.forename: Takafumi
authors.initials: T
authors.lastname: Kaneko
authors.forename: Junji
authors.initials: J
authors.lastname: Watari
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides / Q000276:immunology* / Q000378:metabolism; D002584:Cervix Uteri / Q000166:cytology; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004423:Ear / Q000473:pathology; D005260:Female; D027582:Humulus / Q000737:chemistry*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008198:Lymph Nodes / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D039741:Antigens, Dermatophagoides; D016207:Cytokines; D010936:Plant Extracts; D014867:Water; D007073:Immunoglobulin E