Gene expression patterns in glucose-stimulated podocytes.
To explore the mechanisms of podocyte injury under diabetic conditions, we performed an expression profile in glucose-stimulated podocytes. Differential gene expression profiles between conditionally immortalized mouse podocytes cultured in medium containing 5.6 and 30 mM glucose were measured with oligonucleotide microarrays. Of the genes identified, heme oxygenase-1, vascular endothelial growth factor-A, and thrombospondin-1 showed a consistently increased pattern, whereas angiotensin-converting enzyme-2 and peroxisomal proliferator activator receptor-gamma were down-regulated. These results were validated using real-time PCR and western blotting in podocytes, and with immunohistochemistry on renal tissues from streptozotocin-induced diabetic rats. Not only is this the first report of gene expression profiling of podocyte injury under diabetic conditions, but the identified genes are promising targets for future diabetes research.
Han
Seung Hyeok
SH
Department of Internal Medicine, Yonsei University College of Medicine, 134 Shinchon-Dong Seodaemoon-Gu, Seoul 120-752, Republic of Korea.
Yang
Sanghwa
S
Jung
Dong Sub
DS
Li
Jin Ji
JJ
Kim
Jin Ju
JJ
Kwak
Seung Jae
SJ
Kim
Dong Ki
DK
Moon
Sung Jin
SJ
Lee
Jung Eun
JE
Han
Dae-Suk
DS
Kang
Shin-Wook
SW
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000235:genetics* / Q000473:pathology; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D050199:Podocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005947:Glucose