Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses.
Calcium plays a crucial role as a second messenger in mediating various defense responses under environmental stresses. Calcineurin B-like (CBL) proteins have been implicated as important Ca2+ sensors in plant-specific calcium signaling. Based on the similarity of sequence, ten CBL genes were identified by searching the rice japonica genome database, which were randomly distribute on chromosomes 1, 2, 3, 5, 10 and 12. By semi-quantitative RT-PCR approach the expression pattern of each gene was detected in various organs at the adult stage and seedlings treated with NaCl, PEG and cold stresses or exogenous ABA. The results showed that the induction of each rice CBL gene was not only responsive to different stress conditions, but also organ specific. In vivo targeting experiment revealed that OsCBL8 localized to the plasma membrane, which was consistent with OsCBL4 and SOS3 previously reported. To elucidate the putative function of OsCBL8 gene, transgenic rice plants overexpressing OsCBL8 gene were generated by the Agrobacterium-mediated approach. The OsCBL8 transgenic rice seedlings showed more tolerance to salt stress than non-transgenic seedlings.
Gu
Zhimin
Z
State key lab of crop genetics and germplasm enhancement, Nanjing Agricultural University, Nanjing, 210095, PR China.
Ma
Bojun
B
Jiang
Yan
Y
Chen
Zhiwei
Z
Su
Xuan
X
Zhang
Hongsheng
H
D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects; D000595:Amino Acid Sequence; D019703:Calcineurin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004777:Environment; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D020539:Sequence Analysis, Protein; D012965:Sodium Chloride / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D019703:Calcineurin