Ni-nitrilotriacetic acid-modified quantum dots as a site-specific labeling agent of histidine-tagged proteins in live cells.
Ni-nitrilotriacetic acid (NTA) functionalized CdSe/ZnS quantum dots (QDs) were exploited as a site-specific labeling agent of histidine-tagged biomolecules in live cells; the QDs were found to be water-soluble, aggregation free and stable for several months.
Kim
Junwon
J
Nano/Bio Chemistry Laboratory, Institut Pasteur Korea (IP-K), 39-1 Hawolgok-dong, Seongbuk-gu, 136-791, Seoul, Korea.
Park
Hye-Young
HY
Kim
Jaeseung
J
Ryu
Jiyoung
J
Kwon
Do Yoon
DY
Grailhe
Regis
R
Song
Rita
R
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology*; D006639:Histidine / Q000737:chemistry*; D006801:Humans; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D045663:Quantum Dots; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D011506:Proteins; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid