title: Three-layered membranes of collagen/hydroxyapatite and chitosan for guided bone regeneration.
abstract: The purpose of this study was to design a novel hybrid membrane with optimized properties for guided bone regeneration (GBR). Both the top and bottom layers of the sandwich-structured membrane were composed of collagen containing 20 wt% hydroxyapatite (HA), while the middle layer was made of chitosan. The above three layers were formulated into an integral membrane from their respective slurries through a layer-by-layer filtration process. The phase and composition of the membrane were confirmed by FT-IR and XRD analyses. The observation of its morphology by SEM showed that the membrane had a porous structure and structural integrity. The chitosan layer ensured the high tensile strength and elastic modulus of the membrane, while the presence of the collagen/HA composite layers endowed it with good flexibility and bioactivity. These results suggest that the integrated membrane prepared in this study would have the potential for use as a GBR material.
authors.affiliation: Department of Materials Science and Engineering, Seoul National University, Seoul, 151-744, Korea.
authors.forename: Shu-Hua
authors.initials: SH
authors.lastname: Teng
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Du-Sik
authors.initials: DS
authors.lastname: Shin
authors.forename: Hyoun-Ee
authors.initials: HE
authors.lastname: Kim
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002460:Cell Line; D048271:Chitosan / Q000627:therapeutic use; D003094:Collagen / Q000627:therapeutic use; D017886:Durapatite / Q000627:therapeutic use; D004548:Elasticity; D048091:Guided Tissue Regeneration / Q000379:methods*; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D016062:Porosity; D013718:Tensile Strength
chemical_list: D008567:Membranes, Artificial; D003094:Collagen; D048271:Chitosan; D017886:Durapatite