title: Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP(+)-dependent xylitol dehydrogenase, and xylulokinase.
abstract: We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.
authors.affiliation: Biomass Technology Research Center (BTRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. a-matsushika@aist.go.jp
authors.forename: Akinori
authors.initials: A
authors.lastname: Matsushika
authors.forename: Seiya
authors.initials: S
authors.lastname: Watanabe
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kodaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Makino
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sawayama
mesh_terms: D050542:D-Xylulose Reductase / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000431:Ethanol; D009249:NADP; D014994:Xylose; D050542:D-Xylulose Reductase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D014993:Xylitol