Cultured keratinocytes in fibrin with decellularised dermis close porcine full-thickness wounds in a single step.
The purpose of this study was to assess the feasibility of combined keratinocyte and dermal scaffold transplantation performed in a single step for treatment of full-thickness wounds. Cultured autologous keratinocytes were suspended in fibrin and grafted together with decellularised human dermis (Alloderm) in a porcine animal model, involving 10 animals over 4 weeks. Wound healing was evaluated by planimetry. Histology included morphological analysis as well as immunohistochemistry at regular intervals (1, 2 and 4 weeks). The results showed both successful histo-integration of the in vivo composite grafts and reduced wound contraction, compared with the control group (plain epithelial grafts). Histologically a neo-epithelium originated from the grafted cells on top of the decellularised dermis, as well as a reconstituted basement membrane. After 4 weeks cellular ingrowth into the dermal matrix could be observed. The successful combination of a keratinocyte-fibrin suspension and acellular dermis applied in a single step onto full-thickness wounds resulted in closure.
Bannasch
Holger
H
Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Str. 55, D-79106 Freiburg, Germany. holger.bannasch@uniklinik-freiburg.de
Unterberg
Tim
T
Föhn
Matthias
M
Weyand
Birgit
B
Horch
Raymund E
RE
Stark
G Björn
GB
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen; D005260:Female; D005337:Fibrin / Q000627:therapeutic use*; D007091:Image Processing, Computer-Assisted; D015603:Keratinocytes / Q000473:pathology / Q000637:transplantation*; D023421:Models, Animal; D012867:Skin / Q000293:injuries* / Q000473:pathology; D013552:Swine; D014182:Transplantation, Autologous; D014945:Wound Healing
C440568:Alloderm; D005337:Fibrin; D003094:Collagen