Internal pH indicators for biomolecular NMR.
We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose (1)H NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.
Baryshnikova
Olga K
OK
Department of Biochemistry, University of Alberta, Edmonton, AB, Canada T6G 2H7.
Williams
Thomas C
TC
Sykes
Brian D
BD
D002021:Buffers; D005561:Formates / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D000077489:Piperazine; D010879:Piperazines / Q000737:chemistry; D014325:Tromethamine / Q000737:chemistry
D002021:Buffers; D005561:Formates; D007093:Imidazoles; D010879:Piperazines; D014325:Tromethamine; C030544:formic acid; D000077489:Piperazine; C029899:imidazole