title: Simple preparation of baicalin from Scutellariae Radix.
abstract: A simple, rapid and reproducible method was developed to isolate high-purity baicalin from Scutellariae Radix, the dried roots of Scutellaria baicalensis Georgi. The method involves partition/recrystallization steps without repeated column chromatography or special instruments. Isolated baicalin was characterized by comparisons of TLC, HPLC, IR, MS, and NMR data with an authentic sample. Moreover, beta-glucuronidase hydrolysis of baicalin sequentially yielded glucuronic acid and baicalein as confirmed by co-TLC with authentic samples. The purity of baicalin was more than 97% with yield ca. 8.7% (w/w). The method presented here appears suitable for commercial application.
authors.affiliation: The School of Pharmaceutical Sciences, Ohu University 31-1, Misumidou Tomita-Machi, Kooriyama-shi, Fukushima 963-8611, Japan. e-ohkoshi@pha.ohu-u.ac.jp
authors.forename: Emika
authors.initials: E
authors.lastname: Ohkoshi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nagashima
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Sato
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Fujii
authors.forename: Koohei
authors.initials: K
authors.lastname: Nozawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nagai
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry*; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin