Protection of chlorophyllin against oxidative damage by inducing HO-1 and NQO1 expression mediated by PI3K/Akt and Nrf2.
Green vegetables are thought to have a chemoprotective effect on the basis of epidemiologic evidence. This study investigated whether chlorophyllin (CHL) could induce antioxidant enzymes and confer protection against oxidative damage. The results showed that CHL could induce HO-1 and NQO1 expression in human umbilical vein endothelial cell (HUVEC) in a time- and dose-dependent manner and protect them against hydrogen peroxide caused oxidative damage. The induction of HO-1 and NQO1 by CHL was accompanied with the accumulation of transcription factor Nrf2 in nucleus and the activation of PI3K/Akt signalling pathway. Additionally, the specific inhibitor of PI3K/Akt could obviously decrease not only the induced expression of HO-1 and NQO1 but also the antioxidant effect of CHL. In conclusion, this study proved that CHL exerts antioxidant effect by inducing HO-1 and NQO1 expression mediated by PI3K/Akt and Nrf2. One thinks CHL may have promise to be prophylactic pharmaceuticals without adverse effects.
Zhang
Yanlin
Y
Research Center of Occupational Medicine, Third Hospital of Peking University, Peking, PR China.
Guan
Li
L
Wang
Xifu
X
Wen
Tao
T
Xing
Junjie
J
Zhao
Jinyuan
J
D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival; D002735:Chlorophyllides / Q000737:chemistry*; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis* / Q000737:chemistry; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt; D014471:Umbilical Veins / Q000166:cytology
D000975:Antioxidants; D002735:Chlorophyllides; D005609:Free Radicals; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D019869:Phosphatidylinositol 3-Kinases; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt; C007020:chlorophyllin