Transcriptome analysis of a cDNA library from adult human epididymis.
Mammalian Gene Collection (MGC) verified over 9,000 human full-ORF genes and FLJ Program reported 21,243 cDNAs of which 14,409 were unique ones and 5,416 seemed to be protein-coding. The pity is that epididymis cDNA library was missing in their sequencing target list. Epididymis is a very important male accessory sex organ for sperm maturation and storage. Fully differentiated spermatozoa left from testis acquire their motility and capacity for fertilization via interactions with the epididymal epithelium duct lumen during passage through this convoluted duct. Here, we report that 20,000 clones from a healthy male epididymis cDNA library have been sequenced. The sequencing data provided 8,234 known sequences and 650 unknown cDNA fragments. Hundred and six of 650 unknown cDNA clone inserts were randomly selected for fully sequencing. There were 25 unknown unique sequences and 19 released but unreported sequences came out. By northern blot analysis, four sequences randomly selected from the 19 released sequences with no known function showed positive mRNA signals in epididymis and testis. The signals for three of six from those unknown group showed as epididymis abundant in a region-specific manner but not in the testis and other tissues tested. All the sequencing data will be available on the website www.sdscli.com.
Li
Jian-Yuan
JY
Shandong Research Center of Stem Cell Engineering/Centre Laboratory, Yu-Huang-Ding Hospital, Yan-Tai, Shan-Dong Province, Shandong 264000, People's Republic of China. sdscli@126.com
Wang
Hai-Yan
HY
Liu
Jie
J
Liu
Qiang
Q
Zhang
Jin-Song
JS
Wan
Feng-Chun
FC
Liu
Fu-Jun
FJ
Jin
Shao-Hua
SH
Zhang
Yong-Lian
YL
D000328:Adult; D000465:Algorithms; D003001:Cloning, Molecular; D004822:Epididymis / Q000378:metabolism*; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D008297:Male; D009928:Organ Specificity / Q000235:genetics