title: Development and evaluation of a novel multiplex PCR technology for molecular differential detection of bacterial respiratory disease pathogens.
abstract: The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.
authors.affiliation: CDC, Atlanta, GA 30333, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Benson
authors.forename: Maria L
authors.initials: ML
authors.lastname: Tondella
authors.forename: Julu
authors.initials: J
authors.lastname: Bhatnagar
authors.forename: Maria da Glória S
authors.initials: Mda G
authors.lastname: Carvalho
authors.forename: Jacquelyn S
authors.initials: JS
authors.lastname: Sampson
authors.forename: Deborah F
authors.initials: DF
authors.lastname: Talkington
authors.forename: Anne M
authors.initials: AM
authors.lastname: Whitney
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Mothershed
authors.forename: Lesley
authors.initials: L
authors.lastname: McGee
authors.forename: George
authors.initials: G
authors.lastname: Carlone
authors.forename: Vondguraus
authors.initials: V
authors.lastname: McClee
authors.forename: Jeannette
authors.initials: J
authors.lastname: Guarner
authors.forename: Sherif
authors.initials: S
authors.lastname: Zaki
authors.forename: Surang
authors.initials: S
authors.lastname: Dejsiri
authors.forename: K
authors.initials: K
authors.lastname: Cronin
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Barry S
authors.initials: BS
authors.lastname: Fields
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012137:Respiratory System / Q000382:microbiology; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic