Method for macromolecular colocalization using atomic recombination in dynamic SIMS.
Localizing two or more components of assemblies in biological systems requires both continued development of fluorescence techniques and invention of entirely new techniques. Candidates for the latter include dynamic secondary ion mass spectrometry (D-SIMS). The latest generation of D-SIMS, the Cameca NanoSIMS 50, permits the localization of specific, isotopically labeled molecules and macromolecules in sections of biological material with a resolution in the tens of nanometers and with a sensitivity approaching in principle that of a single protein. Here we use two different systems, crystals of glycine and mixtures of proteins, to show that the formation of recombinant CN secondary ions under Cs bombardment can be exploited to create a new colocalization technique. We show experimentally that the formation of the recombinant (13)C(15)N secondary ion between (13)C- and (15)N-labeled macromolecules is indeed an indicator of the distance between the interacting macromolecules and on their shape. We build up a convolution model of the mixing-recombination process in D-SIMS that allows quantitative interpretations of the distance-dependent formation of the recombinant CN. Our results show that macromolecules can be colocalized if they are within 2 nm of one another. We discuss the potential advantages of this new technique for biological applications.
Legent
G
G
Laboratoire Assemblages moléculaires: modélisation, et imagerie SIMS, Faculté des Sciences de l'Université de Rouen, 76821 Mont Saint Aignan Cedex, France.
Delaune
A
A
Norris
V
V
Delcorte
A
A
Gibouin
D
D
Lefebvre
F
F
Misevic
G
G
Thellier
M
M
Ripoll
C
C
D002247:Carbon Isotopes; D003460:Crystallization; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D005998:Glycine / Q000737:chemistry*; D007477:Ions; D008433:Mathematics; D008954:Models, Biological; D009587:Nitrogen Isotopes; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*
D002247:Carbon Isotopes; D029968:Escherichia coli Proteins; D007477:Ions; D009587:Nitrogen Isotopes; D005998:Glycine