An antagonist of lipid A action in mammals has complex effects on lipid A induction of defence responses in the model plant Arabidopsis thaliana.
Lipopolysaccharides, the ubiquitous part of the outer membrane of Gram-negative bacteria, and their derivatives are recognised by plants to trigger or potentiate particular defence responses such as induction of genes encoding pathogenesis-related proteins. The molecular mechanisms of LPS perception that underpin these effects in plants are, however, unknown. Here, lipid A from Halomonas magadiensis, which is an antagonist of lipid A action in human cells, was used to investigate lipid A action in plants. Our findings offer an insight into the different structural requirements for direct induction and potentiation of plant defences by lipid A.
Erbs
Gitte
G
Faculty of Life Sciences, Department of Plant Biology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Denmark.
Jensen
Tina Tandrup
TT
Silipo
Alba
A
Grant
William
W
Dow
J Maxwell
JM
Molinaro
Antonio
A
Parrilli
Michelangelo
M
Newman
Mari-Anne
MA
D017360:Arabidopsis / Q000382:microbiology*; D004926:Escherichia coli / Q000737:chemistry; D004927:Escherichia coli Infections / Q000382:microbiology; D018506:Gene Expression Regulation, Plant; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D020632:Halomonas / Q000737:chemistry*; D008050:Lipid A / Q000037:antagonists & inhibitors*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D018749:RNA, Plant / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008050:Lipid A; D010940:Plant Proteins; D018749:RNA, Plant; C053376:pathogenesis-related proteins, plant