[Haemolysis and turbidity influence on three analysis methods of quantitative determination of total and conjugated bilirubin on ADVIA 1650].
Plasma bilirubin testing is crucial to prevent the occurrence of neonatal kernicterus. Haemolysis may occur during sampling and interfere with bilirubin determination. Moreover, lipidic infusions may induce plasma lipemia and also interfere with bilirubin measurement. We evaluated the interference of haemolysis and lipemia with three methods of total and direct bilirubin measurement adaptated on an Advia 1650 analyser (Siemens Medical Solutions Diagnostics) : Synermed (Sofibel), Bilirubin 2 (Siemens) and Bilirubin Auto FS (Diasys). The measurement of total bilirubin was little affected by haemolysis with all three methods. The Bilirubin 2 (Siemens) method was the less sensitive to haemolysis even at low bilirubin levels. The measurement of conjugated bilirubin was significantly altered by low heamoglobin concentrations for Bilirubin Auto FS(R) (30 microM or 0,192 g/100 mL haemoglobin) and for Synermed (60 microM or 0,484 g/100 mL haemoglobin). In marked contrast, we found no haemoglobin interference with the Direct Bilirubin 2 reagent which complied with the method validation criteria from the French Society for Biological Chemistry. The lipemia up to 2 g/L of Ivelip did not affect neither the measurement of total bilirubin for all three methods nor the measurement of conjugated bilirubin with the Diasys and Siemens reagents. However, we observed a strong interference starting at 0,5 g/L of Ivelip with the Synermed reagent. Our data suggest that both Siemens and Diasys methods allow to measure accurately total and conjugated bilirubin in hemolytic and lipemic samples, nevertheless, the Siemens methodology is less affected by these interferences.
Gobert De Paepe
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Laboratoire de biochimie-hormonologie, Hôpital Robert Debré, Paris. elisabeth.de-paepe@rdb.aphp.fr
Munteanu
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Schischmanoff
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Porquet
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D001663:Bilirubin / Q000097:blood*; D001664:Biliverdine / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D001800:Blood Specimen Collection; D003627:Data Interpretation, Statistical; D006461:Hemolysis; D006801:Humans; D051556:Hyperbilirubinemia, Neonatal / Q000175:diagnosis*; D007231:Infant, Newborn; D007567:Jaundice, Neonatal / Q000517:prevention & control*; D008055:Lipids / Q000097:blood*; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D008055:Lipids; D001664:Biliverdine; D001663:Bilirubin