Isolation and characterization of a new Arthrospira strain.
A filamentous microorganism, morphologically similar to the cyanobacterium Arthrospira, was isolated from Mangueira Lagoon in Brazil, from which Arthrospira has not previously been isolated. Random amplified polymorphic DNA (RAPD) comparison with the standard Arthrospira platensis strains LEB 52 and Paracas indicated that the organism isolated was an Arthrospira isolate, which we denominated strain LEB 18. The RAPD analysis showed conserved sequences which indicated that the three strains belonged to the same genus, and were all Arthrospira species, but there were sufficient differences between them suggesting that they were separate strains. The strain LEB 18 was cultivated in undiluted Zarrouk medium and in 60% and 20% (v/v) Zarrouk medium diluted with sterilized Mangueira Lagoon water (MLW) using illuminance rates of 32.5, 45.5 and 58.5 micromol m(-2) s(-1) according to a complete 32 factorial design with a triplicate central point. The strains LEB 52 and Paracas were cultived in the conditions central point. Our new isolate produced the highest specific growth rate (Umax = 0.22 d(-1)) in 60% Zarrouk medium diluted with MLW and illuminated with 58.5 micromol m(-2) s(-1) and the highest protein content (86.0% w/w).
de Morais
Michele Greque
MG
Laboratory of Biochemical Engineering, Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil.
da Cruz Reichert
Carolina
C
Dalcanton
Francieli
F
Durante
Andrei José
AJ
Marins
Luis Fernando
LF
Costa
Jorge Alberto Vieira
JA
D001938:Brazil; D000458:Cyanobacteria / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D017931:DNA Primers; D010802:Phylogeny; D014871:Water Microbiology
D017931:DNA Primers