Expression, purification, crystallization and preliminary X-ray diffraction analysis of thioredoxin Trx1 from Saccharomyces cerevisiae.
Thioredoxins play key roles in the cellular response to oxidative stress. Three isoforms of thioredoxin have been identified in Saccharomyces cerevisiae: two that are cytosolic (Trx1 and Trx2) and one that is mitochondrial (Trx3). In the present work, the cytosolic form Trx1 was cloned, expressed, purified and crystallized. Crystals were obtained by the hanging-drop vapour-diffusion method. A data set was collected from a single crystal to 1.7 A resolution. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 32.29, b = 46.59, c = 64.20 A, alpha = beta = gamma = 90 degrees .
Zhang
Yaru
Y
Institute of Protein Research, Tongji University, Shanghai 200092, People's Republic of China.
Bao
Rui
R
Zhou
Cong-Zhao
CZ
Chen
Yuxing
Y
D000595:Amino Acid Sequence; D003460:Crystallization; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D054464:Peroxiredoxins; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013879:Thioredoxins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C103243:TRX1 protein, S cerevisiae; D013879:Thioredoxins; D054464:Peroxiredoxins