Genotypic profiles and virulence attributes of Candida albicans isolates from patients with oral lichen planus.
Candida albicans carriage has been found to be increased in patients with oral lichen planus. In the present work we have investigated the genotypic profiles of 112 C. albicans strains isolated from patients with erosive or nonerosive OLP, and from healthy controls. The virulence attributes of the isolated strains were compared to elucidate the pathogenetic mechanisms through which C. albicans may cause OLP. We have characterized the genotypic profiles of these isolated strains using a randomly amplified polymorphic DNA assay. In addition, we used assays to measure adhesion to buccal epithelial cells and phospholipase activity to evaluate the virulence attributes of these isolates. Our RAPD analyses revealed four different genotypes, named type A to D, among all isolates, and identified statistically significant associations with disease conditions. Specifically, type A (58.1%) and C (29.0%) were primarily found in erosive OLP, while type A (33.3%) and D (58.3%) were identified in nonerosive OLP. However, the healthy group seemed to have type B (38.5%) and D (61.5%). Using adhesion to BEC assay, we demonstrated that the isolates with genotype A had the strongest adherence among the four genotypes (P=0.000<0.05). The phospholipase activity of the isolates with genotype A and C was higher than for those with genotype B and D (P=0.000<0.05). In conclusion, some C. albicans isolates with special genotypic profiles and virulence attributes may contribute to the pathogenesis of OLP.
Zeng
Xin
X
National Key Laboratory of Stomatology and West China College of Stomatology, Sichuan University, Chengdu, Sichuan, China.
Xiong
Chunrong
C
Wang
Zhi
Z
Jiang
Lu
L
Hou
Xiaohui
X
Shen
Jun
J
Zhou
Min
M
Chen
Qianming
Q
D002176:Candida albicans / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D002177:Candidiasis / Q000150:complications* / Q000382:microbiology*; D002448:Cell Adhesion; D018450:Disease Progression; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D017676:Lichen Planus, Oral / Q000150:complications* / Q000382:microbiology* / Q000473:pathology; D009061:Mouth Mucosa / Q000382:microbiology / Q000473:pathology; D016533:Mycological Typing Techniques; D010740:Phospholipases / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D014456:Ulcer / Q000473:pathology; D014774:Virulence
D010740:Phospholipases