Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio.
C1q family proteins with C1q domain have been reported in vertebrates, but their biological roles are currently unknown. In this study, a C1q-like factor, designated Carassius auratus gibelio ovary-specific C1q-like factor (CagOC1q-like), was identified as a cortical granules component. Immunofluorescence localization revealed that the C1q family member was specifically expressed in follicular epithelial cells, and associated with cortical granules in fully grown oocytes. Moreover, it was discharged to the perivitelline space and egg envelope upon fertilization. As it is the first identified C1q family member that is expressed in follicular cells that surround oocyte, CagOC1q-like was applied to detection of follicular cell apoptosis and deletion. The entire cytological process of follicular cell apoptosis and deletion was clearly seen from double visualizations of follicular cells with CagOC1q-like immunofluorescence and apoptotic follicular cells labeled by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) during oocyte maturation and ovulation.
Mei
Jie
J
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Wuhan 430072, China.
Chen
Bo
B
Yue
Huamei
H
Gui
Jian-Fang
JF
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D015922:Complement C1q / Q000235:genetics* / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006054:Goldfish; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008969:Molecular Sequence Data; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D010053:Ovary / Q000166:cytology / Q000378:metabolism*; D017434:Protein Structure, Tertiary
D029941:Fish Proteins; D015922:Complement C1q