title: Lipid-lowering and antioxidant effects of hydroxytyrosol and its triacetylated derivative recovered from olive tree leaves in cholesterol-fed rats.
abstract: This study was designed to test the lipid-lowering and antioxidative activities of triacetylated hydroxytyrosol compared with its native compound, hydroxytyrosol, purified from olive tree leaves. Wistar rats fed a standard laboratory diet or a cholesterol-rich diet for 16 weeks were used. The serum lipid levels, the thiobarbituric acid-reactive substances (TBARS) level, as an indicator of lipid peroxidation, and the activity of superoxide dismutase (SOD) as well as that of catalase (CAT) were examined. The cholesterol-rich diet induced hypercholesterolemia that was manifested in the elevation of total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C). Administration of hydroxytyrosol and triacetylated hydroxytyrosol (3 mg/kg of body weight) decreased the serum levels of TC, TG, and LDL-C significantly and increased the serum level of high-density lipoprotein cholesterol (HDL-C). Furthermore, the content of TBARS in liver, heart, kidney, and aorta decreased significantly when hydroxytyrosol and its triacetylated derivatives were orally administered to rats compared with those fed a cholesterol-rich diet. In addition, triacetylated hydroxytyrosol and hydroxytyrosol increased CAT and SOD activities in the liver. These results suggested that the hypolipidemic effect of triacetylated hydroxytyrosol and hydroxytyrosol might be due to their abilities to lower serum TC, TG, and LDL-C levels as well as to their antioxidant activities preventing the lipid peroxidation process.
authors.affiliation: Laboratoire des Bioprocédés, Pôle d'Excellence Régional AUF, Centre de Biotechnologie de Sfax, B.P. K 3038 Sfax, Tunisia.
authors.forename: Hedya
authors.initials: H
authors.lastname: Jemai
authors.forename: Ines
authors.initials: I
authors.lastname: Fki
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bouaziz
authors.forename: Zouhaier
authors.initials: Z
authors.lastname: Bouallagui
authors.forename: Abdelfattah
authors.initials: A
authors.lastname: El Feki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
mesh_terms: D000107:Acetylation; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002374:Catalase / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000008:administration & dosage*; D000960:Hypolipidemic Agents / Q000008:administration & dosage*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D031658:Olea / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D002791:Cholesterol, Dietary; D000960:Hypolipidemic Agents; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; C005975:3,4-dihydroxyphenylethanol; D002374:Catalase; D013482:Superoxide Dismutase; D010626:Phenylethyl Alcohol