Photo-crosslinkable, biomimetic, and thermo-sensitive pluronic grafted hyaluronic acid copolymers for injectable delivery of chondrocytes.
Hyaluronic acid (HA) grafted with Pluronic F127 copolymer was used as biomimetic hydrogels for cell delivery. The graft copolymer was synthesized by conjugating amine end-capped Pluronic F127 to carboxylic groups of HA using coupling agents. The synthesized HA-g-Pluronic exhibited thermo-sensitive sol-gel transition behaviors over the temperature range of 20-40 degrees C. HA-g-Pluronic copolymers with vinyl groups were photo-crosslinked to prepare more robust hydrogels for cell cultivation. For improved cellular adhesion and proliferation, cell adhesive peptide (Arg-Gly-Asp (RGD)) was additionally conjugated to the HA backbone. The resultant thermo-sensitive, photocrosslinkable, and RGD modified HA-g-Pluronic copolymers were used to encapsulate and cultivate bovine chondrocytes in vitro. A tissue containing cartilage-like components such as GAG and type II collagen was successfully produced within the hydrogels, indicating that the synthesized HA-g-Pluronic copolymers can be potentially used as an injectable cell carrier.
Lee
Hyukjin
H
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea South.
Park
Tae Gwan
TG
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D007267:Injections; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D044367:Phase Transition; D010777:Photochemistry; D011095:Polyethylenes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013696:Temperature
D003432:Cross-Linking Reagents; D011095:Polyethylenes; D011108:Polymers; D011126:Polypropylenes; D006820:Hyaluronic Acid; C078661:UCON 50-HB-5100