title: Effects of green tea fractions on oxygen-induced retinal neovascularization in the neonatal rat.
abstract: This study aimed to investigate the preventive effects of green tea fractions (GTFs) on rat model of oxygen-induced retinopathy (OIR). Neonatal Sprague-Dawley rats were exposed to daily cycles of 80% oxygen (20.5 h), ambient air (0.5 h), and progressive return to 80% oxygen (3 h) until postnatal day 12 (P12), then the rats were placed in ambient air until P18. The green tea was fractionated by DM-A50, DM-W, M-B, and M-W. The rats were treated once daily from P6 to P17 by gastric gavage of GTFs (0.05 or 0.01 g/ml) or distilled water (DW) at 50 microl/10 g body weight. On P18, the rats were sacrificed and the retinal samples were collected. The retinal neovascularization (NV) was scored and avascular areas (AVAs) were measured as a % of total retinal area (%AVAs) in ADPase stained retinas. The NV scores in 0.01 g/ml M-W were significantly lower than those in DW. The %AVAs in 0.05 g/ml DM-A50 and in 0.05 g/ml and 0.01 g/ml M-W were significantly lower than those in DW. There were less catechins, and less caffeine in M-W fraction compared with other GTFs, suggesting components of green tea except for catechins and caffeine might suppress the neovascularization in rat model of OIR.
authors.affiliation: Department of Ophthalmology, School of Medicine, Showa University, Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Saito
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Hasebe-Takenaka
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Ueda
authors.forename: Takako
authors.initials: T
authors.lastname: Nakanishi-Ueda
authors.forename: Shotaro
authors.initials: S
authors.lastname: Kosuge
authors.forename: Masaki
authors.initials: M
authors.lastname: Aburada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Shimada
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Ikeya
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Onda
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Ogura
authors.forename: Yoko
authors.initials: Y
authors.lastname: Taguchi
authors.forename: Hajime
authors.initials: H
authors.lastname: Yasuhara
authors.forename: Ryohei
authors.initials: R
authors.lastname: Koide