In situ detection of programmed cell death in Nicotiana tabacum leaves during senescence.
Leaf senescence is a highly regulated developmental process, during which cell constituents are dismantled in an ordered progression. The final death phase of senescence is a type of programmed cell death (PCD). We showed DNA fragmentation characteristic for PCD by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assay not only in senescing leaves but also in young, still developing tobacco leaves.
Uzelac
B
B
Institute for Biological Research Sinisa Stanković, Bulevar despota Stefana 142, Belgrade, Serbia. branka@ibiss.bg.ac.yu
Janosević
D
D
Budimir
S
S
D000375:Aging; D017209:Apoptosis; D053938:DNA Fragmentation; D018744:DNA, Plant / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008856:Microscopy, Fluorescence; D018515:Plant Leaves / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods; D014026:Nicotiana / Q000502:physiology*
D018744:DNA, Plant