Immunochemotherapy for cryptosporidiosis in immunosuppressed mouse model.
Immunochemotherapy as a dual regimen (Nitazoxanide NTZ and Interferon gamma INF-gamma) and a triple one (NTZ, INF-gamma & Paromomycin PRM), administered to immunosuppressed Cryptosporidium infected mice for 10 days (4th-13th day post-infection) was evaluated during and after treatment by determination of parasite count in ileum, associated histopathological changes, oocyst count in Kinyoun's acid fast stained faecal smears, percent reduction in oocyst excretion and cure rate. Both regimens induced nearby efficacy (P > 0.05) with significant reduction in parasite count in the ileum on 7th (P < 0.01) & 14th (P < 0.001) P.I. days, partial regression of histopathological changes and reduction in oocyst count from the 2nd day post-treatment. Oocyst excretion reduction percent was reached zenith on 13th P.I day in both dual (95.76%) and triple (94.86%) regimens (P > 0.05). Complete cure was not achieved. Three days post-treatment relapse occurred in both regimens (P < 0.001) increase in oocyst count (P < 0.01) increase in parasite count in ileum, more severe histopathological changes with rapid deterioration and then, death of all remaining treated mice
Sanad
Magda M
MM
Department of Biology, Colleges of Education, Al-Riyadh University for Girls, Saudi Arabia. sand_mm2000@yahoo.com
Al-Malki
Jamila S
JS
D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology; D003457:Cryptosporidiosis / Q000188:drug therapy / Q000628:therapy*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D006801:Humans; D016867:Immunocompromised Host; D007167:Immunotherapy; D007371:Interferon-gamma / Q000494:pharmacology; D008018:Life Cycle Stages; D051379:Mice; D009574:Nitro Compounds; D033661:Oocysts / Q000187:drug effects; D010270:Parasite Egg Count; D010303:Paromomycin / Q000494:pharmacology*; D011897:Random Allocation; D013844:Thiazoles / Q000494:pharmacology*; D013997:Time Factors; D016896:Treatment Outcome
D000981:Antiprotozoal Agents; D009574:Nitro Compounds; D013844:Thiazoles; D010303:Paromomycin; D007371:Interferon-gamma; C041747:nitazoxanide