title: Intraperitoneal administration of gonadotropin-releasing hormone-PE40 induces castration in male rats.
abstract: OBJECTIVE
  To evaluate the long-term effects of gonadotropinreleasing hormone (GnRH)-based vaccine on levels of GnRH antibody and testosterone, and vaccine-induced immunocastration on sexual behavior of male rats.
  
  
  METHODS
  The rats were treated with GnRH-PE40 intraperitoneally every other day for 12 wk. GnRH antibody and testosterone level in rat blood were determined by ELISA and radioimmunoassay, respectively. Morphological changes in testes and sexual behavior of rats were evaluated.
  
  
  RESULTS
  GnRH-PE40 induced a high production in GnRH antibody, decreased the serum testosterone level, testis atrophy and sexual function in rats.
  
  
  CONCLUSIONS
  Intraperitoneal administration of GnRHPE40 produces structural and functional castration of male rat reproductive system by inducing anti-GnRH antibody.
authors.affiliation: Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, Guangdong Province, China. doctoryuli@yahoo.com.cn
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Zhong-Fang
authors.initials: ZF
authors.lastname: Zhang
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Jing
authors.forename: Feng-Lin
authors.initials: FL
authors.lastname: Wu
mesh_terms: D000818:Animals; D002369:Castration; D005098:Exotoxins / Q000378:metabolism* / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000737:chemistry*; D007274:Injections, Intraperitoneal; D008297:Male; D010455:Peptides / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012726:Sexual Behavior, Animal / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D005098:Exotoxins; C120463:L-GnRH-PE40; D010455:Peptides; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone