Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle.
Hepatitis E virus (HEV) accounts for the majority of enterically transmitted hepatitis infections worldwide. Currently, there is no specific treatment for or vaccine against HEV. The major structural protein is derived from open reading frame (ORF) 2 of the viral genome. A potential oral vaccine is provided by the virus-like particles formed by a protein construct of partial ORF3 protein (residue 70-123) fused to the N-terminus of the ORF2 protein (residues 112-608). Single crystals obtained by the hanging-drop vapour-diffusion method at 293 K diffract X-rays to 8.3 A resolution. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 337, b = 343, c = 346 A, alpha = beta = gamma = 90 degrees , and contain one particle per asymmetric unit.
Wang
Che-Yen
CY
Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
Miyazaki
Naoyuki
N
Yamashita
Tetsuo
T
Higashiura
Akifumi
A
Nakagawa
Atsushi
A
Li
Tian-Cheng
TC
Takeda
Naokazu
N
Xing
Li
L
Hjalmarsson
Erik
E
Friberg
Claes
C
Liou
Der-Ming
DM
Sung
Yen-Jen
YJ
Tsukihara
Tomitake
T
Matsuura
Yoshiharu
Y
Miyamura
Tatsuo
T
Cheng
R Holland
RH
D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D003460:Crystallization; D016752:Hepatitis E virus / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D014771:Virion / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
D036022:Capsid Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins