Theoretical analysis of the importance of recycling in measurements of protein turnover by constant infusion of a labelled amino acid.
In studies of whole body protein turnover, recycling of tracer from the breakdown of labelled protein is usually neglected; this neglect may introduce a significant error. A three-pool model with fast and slowly turning over protein pools has been used to calculate recycling rates over a range of sizes and turnover rates of the protein pools. Complete and approximate solutions of the equations are given. The recycling rate of 1% per hour would fit the available data on the turnover rates of human tissue proteins.
Slevin
Keith
K
Department of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. slevin@phys.sci.osaka-u.ac.jp
Waterlow
John
J
D000465:Algorithms; D000596:Amino Acids / Q000494:pharmacology*; D004044:Dietary Proteins / Q000008:administration & dosage; D006801:Humans; D007553:Isotope Labeling; D008954:Models, Biological; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*
D000596:Amino Acids; D004044:Dietary Proteins; D011506:Proteins