Analysis of lectin concentrations in different Rhizoctonia solani strains.
Lectins are carbohydrate-binding proteins that contain at least one carbohydrate binding domain which can bind to a specific mono- or oligosaccharide. These proteins are widely distributed in plants. However, over the last decade evidence is accumulating that lectins occur also in numerous fungi belonging to both the Ascomycota and Basiodiomycota. Rhizoctonia solani is known to be an important pathogen to a wide range of host plants. In this study, isolates of R. solani from different anastomosis groups have been screened for the presence of lectin using agglutination assays to detect and quantitate lectin activity. The evaluation included determination of the lectin content in mycelium as well as in sclerotia. The amount of lectin in the sclerotia was higher than in the mycelium of the same strains. The R. solani strains with the highest amounts of lectin have been selected for cultivation, extraction and purification of the lectin.
Hamshou
M
M
Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, BE-9000 Gent, Belgium.
Smagghe
G
G
Van Damme
E J M
EJ
D000372:Agglutination Tests; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis* / Q000302:isolation & purification; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification; D025282:Mycelium / Q000737:chemistry; D010572:Pest Control, Biological; D012232:Rhizoctonia / Q000737:chemistry*
D005656:Fungal Proteins; D037102:Lectins