Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays.
As drug delivery, therapy, and medical imaging are becoming increasingly cell-specific, there is a critical need for high fidelity and high-throughput screening methods for cell surface interactions. Cell membrane-mimicking surfaces, i.e., supported lipid bilayers (SLBs), are currently not sufficiently robust to meet this need. Here we describe a method of forming fluidic and air-stable SLBs through tethered and dispersed cholesterol groups incorporated into the bottom leaflet. Achieving air stability allows us to easily fabricate SLB microarrays from direct robotic spotting of vesicle solutions. We demonstrate their application as cell membrane-mimicking microarrays by reconstituting peripheral as well as integral membrane components that can be recognized by their respective targets. These demonstrations establish the viability of the fluidic and air-stable SLB platform for generating content microarrays in high throughput studies, e.g., the screening of drugs and nanomedicine targeting cell surface receptors.
Deng
Yang
Y
Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Wang
Yini
Y
Holtz
Bryan
B
Li
Jingyi
J
Traaseth
Nathan
N
Veglia
Gianluigi
G
Stottrup
Benjamin J
BJ
Elde
Robert
R
Pei
Duanqing
D
Guo
Athena
A
Zhu
X-Y
XY
D040761:Biomimetic Materials / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D002788:Cholesterol Esters / Q000737:chemistry / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005677:G(M1) Ganglioside / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods*; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000737:chemistry / Q000378:metabolism; D000099211:Phospholamban
D002135:Calcium-Binding Proteins; D002788:Cholesterol Esters; D005229:Fatty Acids, Monounsaturated; D005677:G(M1) Ganglioside; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D000644:Quaternary Ammonium Compounds; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000099211:Phospholamban; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane