[Coriaria lactone induces in vitro the overexpression of multidrug resistance P-glycoprotein in astrocyte from rat brain].
OBJECTIVE
The aim of this study was to explore the possible pharmacoresistant mechanism by developing a kind of astrocyte model induced by Coriaria lactone (CL).


METHODS
Astrocyte cells from natal Sprague-Dawley rats (within 24 hours of birth) were cultured. Different volumes of CL (1 microL, 3 microL, 9 microL, 15 microL) were added to the cultures for 7, 14, 21 or 28 days. The expression of P-glycoprotein (Pgp), the protein product of multidrug resistance 1 (MDR1), was investigated with immunocytochemistry and image analysis.


RESULTS
CL induced the overexpression of Pgp in astrocytes in a time-dependent manner. The up-regulation of Pgp expression was detected to any concentration of CL from day 14 to 28 (P < 0.01). On the day 21, 28, the up-regulation of Pgp expression level was no dose-dependent manner (P > 0.05).


CONCLUSIONS
The study provides a ideal astrocyte model of pharmacoresistant epilepsy, which is induced by CL. The increased expressions of P-gp may involve in induction of the pharmacoresistance.
Geng
Jia
J
Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Zhou
Dong
D
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007783:Lactones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004365:Drugs, Chinese Herbal; D007783:Lactones; C049836:coriaria lactone