CLEC9A is a novel activation C-type lectin-like receptor expressed on BDCA3+ dendritic cells and a subset of monocytes.
We describe here the first characterization of CLEC9A, a group V C-type lectin-like receptor located in the "Dectin-1 cluster" of related receptors, which are encoded within the natural killer (NK)-gene complex. Expression of human CLEC9A is highly restricted in peripheral blood, being detected only on BDCA3(+) dendritic cells and on a small subset of CD14(+)CD16(-) monocytes. CLEC9A is expressed at the cell surface as a glycosylated dimer and can mediate endocytosis, but not phagocytosis. CLEC9A possesses a cytoplasmic immunoreceptor tyrosine-based activation-like motif that can recruit Syk kinase, and we demonstrate, using receptor chimeras, that this receptor can induce proinflammatory cytokine production. These data indicate that CLEC9A functions as an activation receptor.
Huysamen
Cristal
C
Division of Immunology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, 7925, South Africa.
Willment
Janet A
JA
Dennehy
Kevin M
KM
Brown
Gordon D
GD
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003713:Dendritic Cells / Q000166:cytology*; D004705:Endocytosis; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D017452:Receptors, IgG / Q000096:biosynthesis; D011975:Receptors, Mitogen / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D000072377:Syk Kinase
C530206:CLEC9a protein, human; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D011975:Receptors, Mitogen; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; C000606897:Syk protein, mouse