title: Overexpression of fucosyltransferase VII (FUT7) promotes embryo adhesion and implantation.
abstract: OBJECTIVE
  To investigate the effect of increased fucosyltransferase VII (FUT7) on sialyl Lewis X (sLeX) synthesis and their impact on embryo implantation.
  
  
  METHODS
  Cell and animal study.
  
  
  METHODS
  Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Department of Biochemistry and Molecular Biology, Dalian Medical University.
  
  
  METHODS
  Adult female mice of Kunming species.
  
  
  METHODS
  A pIRES2-EGFP-FUT7 expression plasmid containing full-length human FUT7 cDNA was constructed and transiently transfected into RL95-2 or injected into the uteri of early pregnant mice. Control subjects received the mock pIRES2-EGFP or anti-sLeX antibody.
  
  
  METHODS
  The expression of FUT7 and sLeX was detected by reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence. The embryo adhesion rate was assayed in vitro with the cocultured RL95-2 and JAR implantation model. In vivo embryo implantation was investigated using a mouse model.
  
  
  RESULTS
  Expression of FUT7 was significantly increased after transfection of pIRES2-EGFP-FUT7 into RL95-2 cells compared with the parental control and mock vector transfectants, as demonstrated by fluorescent microscopy and RT-PCR. Expression of sLeX was also increased in FUT7 overexpressers, as detected by indirect immunofluorescence. Consistent with the elevated expression of FUT7 and sLeX, embryo adhesion rate and embryo implantation competence were significantly increased (P<.01) in both in vitro and in vivo models.
  
  
  CONCLUSIONS
  Overexpression of FUT7 up-regulates sLeX synthesis and enhances sLeX-mediated embryo implantation.
authors.affiliation: Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Yuejian
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhongshan
authors.initials: Z
authors.lastname: Wang
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Wang
authors.forename: Qiu
authors.initials: Q
authors.lastname: Yan
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D018920:Coculture Techniques; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D009844:Oligosaccharides / Q000378:metabolism*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000080506:Sialyl Lewis X Antigen; D014162:Transfection; D015854:Up-Regulation; D014599:Uterus / Q000201:enzymology*
chemical_list: D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; C502597:FUT7 protein, human; D005647:Fucosyltransferases