title: Preparation of molecularly imprinted solid phase extraction using bensulfuron-methyl imprinted polymer and clean-up for the sulfonylurea-herbicides in soybean.
abstract: A pre-treatment methodology based on the molecularly imprinted solid phase extraction (MI-SPE) procedure was developed for the determination of bensulfuron-methyl (BSM), tribenuron-methyl (TBM), metsulfuron-methyl (MSM) and nicosulfuron (NS) in soybean samples. A molecular imprinted polymer (MIP) was prepared by precipitation polymerization using BSM as the template molecule, alpha-methacrylic acid (MAA) as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the cross-linker and dichloromethane as the porogen. The binding behaviors of the template BSM and its analogues on the MIP were evaluated by high performance liquid chromatography (HPLC). Then, solid phase extraction (SPE) with a BSM molecularly imprinted polymer (BSM-MIP) as adsorbent was investigated and the optimum loading, washing, and eluting conditions for MI-SPE of the selected BSM, MSM, TBM, and NS were established. The optimized MI-SPE procedure was used to extract the sulfonylureas and a high recovery was obtained in the soybean samples.
authors.affiliation: State Key Laboratory of Food Science & Technology, Jiangnan University, Jiangsu, Wuxi 214122, China. tkjie999@sina.com
authors.forename: Kaijie
authors.initials: K
authors.lastname: Tang
authors.forename: Shangwei
authors.initials: S
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Gu
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.forename: Jian
authors.initials: J
authors.lastname: Tang
mesh_terms: D002138:Calibration; D004566:Electrodes; D006540:Herbicides / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*; D013453:Sulfonylurea Compounds / Q000032:analysis* / Q000737:chemistry
chemical_list: D006540:Herbicides; D011108:Polymers; D013453:Sulfonylurea Compounds; C071475:methyl bensulfuron