title: HLA class I and II gene polymorphisms in Stevens-Johnson syndrome with ocular complications in Japanese.
abstract: OBJECTIVE
  Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are acute-onset mucocutaneous diseases induced by infectious agents and/or inciting drugs. Although the pathobiological mechanisms underlying the onset of SJS/TEN have not been fully established, the extreme rarity of cutaneous and ocular surface reactions to drug therapies led us to suspect individual susceptibility. Our previous study of polymorphisms in the HLA-class I genes of 40 Japanese SJS/TEN patients with ocular surface complications showed that in the Japanese, HLA-A*0206 was strongly associated with SJS/TEN. In this study, we investigated the association between HLA class II antigens in addition to HLA class I antigens and SJS/TEN.
  
  
  METHODS
  We studied the histocompatibility antigen genes, HLA-A, B, C, DRB1, and DQB1, of 71 Japanese SJS/TEN patients with ocular complications. We also genotyped 113 healthy volunteers for HLA-A, B, C, DRB1, and DQB1. We performed polymerase chain reaction amplification followed by hybridization with sequence-specific oligonucleotide probes (PCR-SSO) using commercial bead-based typing kits.
  
  
  RESULTS
  HLA-A*0206 was strongly associated with SJS/TEN. HLA-A*1101 was inversely associated. HLA-B*5901 exhibited a high odds ratio for SJS/TEN with ocular complications. However, when we corrected the p-value for the number of alleles detected (n=29), the results ceased to be significant. There was no association between HLA-C and SJS/TEN. There was also no significant association between HLA-DRB1 and SJS/TEN. HLA-DQB1*0502 was negatively and weakly associated with SJS/TEN although correction of the p-value for the number of alleles detected rendered the result not significant.
  
  
  CONCLUSIONS
  Because our findings are completely different from data reported on Caucasian patients, they suggest strong ethnic differences in the HLA-SJS associations.
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. mueta@ophth.kpu-m.ac.jp
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ueta
authors.forename: Katsushi
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Chie
authors.initials: C
authors.lastname: Sotozono
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Inatomi
authors.forename: Toshio
authors.initials: T
authors.lastname: Yabe
authors.forename: Masaki
authors.initials: M
authors.lastname: Matsushita
authors.forename: Yoko
authors.initials: Y
authors.lastname: Mitsuishi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D005128:Eye Diseases / Q000209:etiology*; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics; D015788:HLA-A1 Antigen; D015789:HLA-A2 Antigen; D006683:HLA-DQ Antigens / Q000235:genetics; D059866:HLA-DQ beta-Chains; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013262:Stevens-Johnson Syndrome / Q000150:complications* / Q000235:genetics*
chemical_list: D015234:HLA-A Antigens; C506470:HLA-A*01:01 antigen; C526286:HLA-A*02:06 antigen; D015788:HLA-A1 Antigen; D015789:HLA-A2 Antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II