title: ASPicDB: a database resource for alternative splicing analysis.
abstract: BACKGROUND
  Alternative splicing has recently emerged as a key mechanism responsible for the expansion of transcriptome and proteome complexity in human and other organisms. Although several online resources devoted to alternative splicing analysis are available they may suffer from limitations related both to the computational methodologies adopted and to the extent of the annotations they provide that prevent the full exploitation of the available data. Furthermore, current resources provide limited query and download facilities.
  
  
  RESULTS
  ASPicDB is a database designed to provide access to reliable annotations of the alternative splicing pattern of human genes and to the functional annotation of predicted splicing isoforms. Splice-site detection and full-length transcript modeling have been carried out by a genome-wide application of the ASPic algorithm, based on the multiple alignments of gene-related transcripts (typically a Unigene cluster) to the genomic sequence, a strategy that greatly improves prediction accuracy compared to methods based on independent and progressive alignments. Enhanced query and download facilities for annotations and sequences allow users to select and extract specific sets of data related to genes, transcripts and introns fulfilling a combination of user-defined criteria. Several tabular and graphical views of the results are presented, providing a comprehensive assessment of the functional implication of alternative splicing in the gene set under investigation. ASPicDB, which is regularly updated on a monthly basis, also includes information on tissue-specific splicing patterns of normal and cancer cells, based on available EST sequences and their library source annotation.
  
  
  BACKGROUND
  www.caspur.it/ASPicDB
authors.affiliation: Consorzio Interuniversitario per le Applicazioni di Supercalcolo per Università e Ricerca, CASPUR, Rome, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Castrignanò
authors.forename: M
authors.initials: M
authors.lastname: D'Antonio
authors.forename: A
authors.initials: A
authors.lastname: Anselmo
authors.forename: D
authors.initials: D
authors.lastname: Carrabino
authors.forename: A
authors.initials: A
authors.lastname: D'Onorio De Meo
authors.forename: A M
authors.initials: AM
authors.lastname: D'Erchia
authors.forename: F
authors.initials: F
authors.lastname: Licciulli
authors.forename: M
authors.initials: M
authors.lastname: Mangiulli
authors.forename: F
authors.initials: F
authors.lastname: Mignone
authors.forename: G
authors.initials: G
authors.lastname: Pavesi
authors.forename: E
authors.initials: E
authors.lastname: Picardi
authors.forename: A
authors.initials: A
authors.lastname: Riva
authors.forename: R
authors.initials: R
authors.lastname: Rizzi
authors.forename: P
authors.initials: P
authors.lastname: Bonizzoni
authors.forename: G
authors.initials: G
authors.lastname: Pesole
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003196:Computer Graphics; D003628:Database Management Systems; D030541:Databases, Genetic; D016247:Information Storage and Retrieval / Q000379:methods; D008969:Molecular Sequence Data; D022821:RNA Splice Sites / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014584:User-Computer Interface
chemical_list: D022821:RNA Splice Sites