title: Wilms' tumor 1 protein and focal adhesion kinase mediate keratinocyte growth factor signaling in breast cancer cells.
abstract: BACKGROUND
  Keratinocyte growth factor (KGF) has been shown to induce breast cancer metastasis in animal models. cDNA microarrays have revealed that KGF increased Wilms tumor 1 (WT1) and focal adhesion kinase (FAK) expression in breast cancer cells. The role of WT1 and FAK in KGF signaling was investigated.
  
  
  METHODS
  A cell culture wounding model was used to study the effects of WT1 and FAK down-regulation on KGF-induced proliferation and motility in breast cancer cells.
  
  
  RESULTS
  WT1 down-regulation inhibited KGF-mediated proliferation and motility of breast cancer cells, while FAK down-regulation inhibited proliferation, but had no significant effect on cell motility. WT1 down-regulation, but not FAK down-regulation, led to Erk1,2 inactivation.
  
  
  CONCLUSIONS
  KGF-mediated signaling employs WT1 and FAK to regulate breast cancer cell proliferation and motility and may represent therapeutic targets for the prevention of breast cancer progression.
authors.affiliation: Department of Pharmaceutical Sciences, University of Oklahoma, Health Sciences Center, Oklahoma City, OK, USA.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zang
authors.forename: J Thomas
authors.initials: JT
authors.lastname: Pento
authors.forename: Ana M
authors.initials: AM
authors.lastname: Tari
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D015536:Down-Regulation; D004789:Enzyme Activation; D051523:Fibroblast Growth Factor 7 / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000378:metabolism*; D051380:GRB2 Adaptor Protein / Q000096:biosynthesis; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D010766:Phosphorylation; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction
chemical_list: D018797:Cell Cycle Proteins; D051380:GRB2 Adaptor Protein; C495948:GRB2 protein, human; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D011994:Recombinant Proteins; C419554:WTAP protein, human; D051523:Fibroblast Growth Factor 7; D051416:Focal Adhesion Protein-Tyrosine Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3