Synthesis of a series of fructooligosaccharides with sucrose and cycloinulohexaose extending over ten degrees of polymerization using cycloinulooligosaccharide fructanotransferase from Bacillus circulans OKUMZ 31B.
Prolonged incubation with sucrose as an acceptor and cycloinulohexaose as a donor at a high concentration of cycloinulooligosaccharide fructanotransferase afforded a series of fructooligosaccharides. Their chain-length distribution extended over 10 degrees of polymerization when the donor concentration was increased to 120 mM. Increasing the acceptor concentration proved effective in improving the yield of inulin-type oligosaccharides because hydrolysis was suppressed.
Kawamura
Mishio
M
Department of Biology, Osaka Kyoiku University, Kashiwara, Osaka 582-8582, Japan. kawamura@cc.osaka-kyoiku.ac.jp
Matsuda
Natsue
N
D001407:Bacillus / Q000201:enzymology*; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005632:Fructose / Q000031:analogs & derivatives* / Q000378:metabolism; D006602:Hexosyltransferases / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D013395:Sucrose / Q000737:chemistry*; D013997:Time Factors
D001704:Biopolymers; D009844:Oligosaccharides; D005632:Fructose; D013395:Sucrose; D006602:Hexosyltransferases; C062589:cycloinulo-oligosaccharide fructanotransferase