title: Microbial production of dihydroxyacetone.
abstract: Dihydroxyacetone is extensively used in cosmetic industry as an artificial suntan besides having clinical and biological applications. Thus, it is important to meet the commercial demand of dihydroxyacetone at an economical and qualitative level. Microbial route of production is found to be more favorable for dihydroxyacetone as compared to chemical methods. This review gives detailed information about the microbial route of dihydroxyacetone production. Till date the microorganism which is most utilized for dihydroxyacetone production is Gluconobacter oxydans. Some limitations associated with dihydroxyacetone production by G. oxydans like substrate inhibition, product inhibition and oxygen limitation are discussed here. Various fermentation modes and culture conditions have been tried for their ability to overcome these limitations. It has been found that fed-batch mode of fermentation provides a better yield as compared to batch mode for dihydroxyacetone production. Two-stage repeated fed-batch mode of fermentation has been found to be the most optimized mode. Immobilization has also been recognized as a much better alternative for fermentation since it avoids the problem of substrate and product inhibition to a greater extent. Although these methods have increased the dihydroxyacetone production to a prominent level yet the production has not reached the level required to meet the commercial demand. One looks for future prospects of developing recombinant microbial method for dihydoxyacetone production.
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016-Kanpur, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Mishra
authors.forename: Seema Rani
authors.initials: SR
authors.lastname: Jain
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
mesh_terms: D001419:Bacteria / Q000166:cytology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004098:Dihydroxyacetone / Q000096:biosynthesis* / Q000138:chemical synthesis; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism; D006801:Humans
chemical_list: D004098:Dihydroxyacetone; D005990:Glycerol