title: DNA strand breaks in grass shrimp embryos exposed to highway runoff sediments and sediments with coal fly ash.
abstract: Embryo production was reduced in female grass shrimp exposed to sediments with added coal fly ash and to sediments collected from an estuarine station containing high PAH concentrations due to its proximity to a highway storm drain. Grass shrimp embryos exposed to pore water from the high PAH and high metal sediments showed both reduced hatching and increases in DNA strand breaks (comet assay). Sediments with added coal fly ash had high concentrations of vanadium and selenium which may have contributed to effects similar to those observed with sediments with high PAH. The embryo pore water bioassay (hatching/DNA strand breaks) gave results comparable to those observed for reproduction effects (reduced embryo production/embryo hatching) with female grass shrimp exposed to whole sediment.
authors.affiliation: Skidaway Institute of Oceanography, 10 Ocean Science Circle, Savannah, GA 31411, USA. dick.lee@skio.usg.edu
authors.forename: Richard F
authors.initials: RF
authors.lastname: Lee
authors.forename: Luis Felipe H
authors.initials: LF
authors.lastname: Niencheski
authors.forename: Karrie
authors.initials: K
authors.lastname: Brinkley
mesh_terms: D000818:Animals; D001681:Biological Assay; D002244:Carbon / Q000633:toxicity*; D060729:Coal Ash; D020552:Comet Assay; D053960:DNA Breaks / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D019015:Geologic Sediments / Q000737:chemistry*; D033541:Palaemonidae / Q000187:drug effects*; D052638:Particulate Matter / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D060729:Coal Ash; D052638:Particulate Matter; D014874:Water Pollutants, Chemical; D002244:Carbon