title: Cysteinyl-leukotrienes in nasal lavage fluid in children with asthma.
abstract: The aim of this study was to investigate repeatability of cysteinyl-leukotrienes (cys-LT) measurements in nasal lavage fluid (NLF) and to determine if cys-LT levels in NLF are related to asthma severity in children. As a second outcome, we investigated if cys-LT in NLF reflect lower airway inflammation as assessed by exhaled NO measurement. To assess the repeatability of cys-LT measurements, two NLF samples were obtained from eight healthy controls 24 h apart. Sixty-nine asthmatic children (mean age; range: 12.8; 7.3-17.7 yr), which were grouped according to asthma severity were studied cross-sectionally on one occasion. Cys-LT in NLF were analyzed using a specific enzyme immunoassay, exhaled NO, and pulmonary function parameters were measured. The coefficient of repeatability for the repeated cys-LT measurements was 1.45 pg/ml. Cys-LT levels in NLF differed significantly between asthma severity groups (p < 0.001): mild intermittent: [median (IQR)] 6.88 pg/ml (2.00-27.87); mild persistent: 21.09 pg/ml (4.50-84.67); and moderate persistent asthmatics: 36.41 pg/ml (11.03-118.40). Concentration of cys-LT in NLF and exhaled NO was positively correlated (r = 0.85; p < 0.001). In conclusion, concentration of cys-LT in NLF correlates with asthma severity in children and is related to lower airway inflammation.
authors.affiliation: Department of Paediatric Surgery, Donauspital, Vienna, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Singer
authors.forename: Friedrich
authors.initials: F
authors.lastname: Horak
authors.forename: Waltraud
authors.initials: W
authors.lastname: Friesenbichler
authors.forename: Barbara
authors.initials: B
authors.lastname: Schneider
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Szépfalusi
authors.forename: Thomas
authors.initials: T
authors.lastname: Frischer
mesh_terms: D000293:Adolescent; D001249:Asthma / Q000175:diagnosis*; D001944:Breath Tests; D002648:Child; D003430:Cross-Sectional Studies; D003545:Cysteine / Q000032:analysis*; D005260:Female; D006801:Humans; D015289:Leukotrienes / Q000032:analysis*; D008297:Male; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D015289:Leukotrienes; C112381:cysteinyl-leukotriene; D009569:Nitric Oxide; D003545:Cysteine