title: Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus.
abstract: Three phenylethanoid glycosides, forsythoside B (1), phlinoside C (2) and verbascoside (3), were isolated from the methanol extract of the leaves of Phlomis lanceolata, an Iranian medicinal plant, by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated conclusively by ultraviolet (UV), mass spectrometry (MS) and a series of 1D and 2D nuclear magnetic resonance (NMR) analyses. The antibacterial properties of 1-3 against five multi-drug-resistant (MDR) strains of Staphylococcus aureus have been assessed by the rapid and robust microtitre-plate-based serial dilution method. While compounds 1 and 3 showed considerable activities against all five strains, compound 2 was inactive at the test concentrations.
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medicinal Sciences, Tabriz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Nazemiyeh
authors.forename: M Mukhlesur
authors.initials: MM
authors.lastname: Rahman
authors.forename: Simon
authors.initials: S
authors.lastname: Gibbons
authors.forename: Lutfun
authors.initials: L
authors.lastname: Nahar
authors.forename: Abbas
authors.initials: A
authors.lastname: Delazar
authors.forename: Mohammed-Ali
authors.initials: MA
authors.lastname: Ghahramani
authors.forename: Amir-Hossein
authors.initials: AH
authors.lastname: Talebpour
authors.forename: Satyajit D
authors.initials: SD
authors.lastname: Sarker
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D024901:Drug Resistance, Multiple, Bacterial; D005960:Glucosides / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010636:Phenols / Q000494:pharmacology; D031345:Phlomis / Q000737:chemistry; D018515:Plant Leaves; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D059808:Polyphenols
chemical_list: D000900:Anti-Bacterial Agents; D005960:Glucosides; D006027:Glycosides; D010636:Phenols; C064692:phlinoside C; C058956:acteoside; D059808:Polyphenols; C058049:forsythiaside