title: ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis.
abstract: Arabidopsis encodes six nuclear actin-related proteins (ARPs), among them ARP8 is unique in having an F-box domain and an actin homology domain. Analysis of the ARP8 promoter-beta-glucuronidase (GUS) fusion suggests that ARP8 is ubiquitously expressed in all organs and cell types. Immunocytochemical analysis with ARP8-specific monoclonal antibodies revealed that ARP8 protein is localized to the nucleolus in interphase cells and dispersed in the cytoplasm in mitotic cells. The cell cycle-dependent subcellular patterns of distribution of ARP8 are conserved in other members of Brassicaceae. Our findings provide the first insight into the possible contributions of plant ARP8 to nucleolar functions.
authors.affiliation: Genetics Department, Davison Life Sciences Building, University of Georgia, Athens, GA 30602, USA.
authors.forename: Muthugapatti K
authors.initials: MK
authors.lastname: Kandasamy
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: McKinney
authors.forename: Richard B
authors.initials: RB
authors.lastname: Meagher
mesh_terms: D000918:Antibody Specificity; D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001937:Brassica / Q000378:metabolism; D002466:Cell Nucleolus / Q000378:metabolism*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter; D021381:Protein Transport
chemical_list: D029681:Arabidopsis Proteins; C529875:Arp8 protein, Arabidopsis; D044783:F-Box Proteins