title: Determination of amikacin in cerebrospinal fluid by high-performance liquid chromatography with pulsed electrochemical detection.
abstract: A highly sensitive and fast reversed-phase liquid chromatographic (LC) method combined with pulsed electrochemical detection (PED) was developed for the direct quantification of the aminoglycoside antibiotic amikacin in cerebrospinal fluid (CSF). The limit of quantification obtained was 0.06 microg/ml and linearity was established over the concentration range 0.06-4.00 microg/ml. The recovery was found to be close to 100%. This method was developed in order to study CSF pharmacokinetics of amikacin in neonates. The narrow therapeutic range calls for monitoring to ensure optimal therapy and to minimize the risk of toxic side effects such as nephro- and ototoxicity, especially in populations like preterm neonates at birth, where the predictability of amikacin clearance is limited. Typical problems to be solved were the low amikacin concentrations and the limited sample volume of CSF.
authors.affiliation: Laboratorium voor Farmaceutische Analyse, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, O&N 2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
authors.forename: Gordana
authors.initials: G
authors.lastname: Brajanoski
authors.forename: Jos
authors.initials: J
authors.lastname: Hoogmartens
authors.forename: Karel
authors.initials: K
authors.lastname: Allegaert
authors.forename: Erwin
authors.initials: E
authors.lastname: Adams
mesh_terms: D000583:Amikacin / Q000134:cerebrospinal fluid* / Q000493:pharmacokinetics; D000900:Anti-Bacterial Agents / Q000134:cerebrospinal fluid* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D000583:Amikacin