title: A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen.
abstract: It has been proposed that activation of Toll-like receptors (TLRs) plays crucial roles in the polarization of adaptive immune responses. A synthetic Toll-like receptor 2 (TLR2) ligand, Pam3CSK4, has been reported to modulate the balance of Th1/Th2 responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized to house dust mite allergen (HDM). Mice were sensitized and challenged with Dermatophagoides farinae allergen (Der f), and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored. Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid (BALF). Serum level of total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells in BALF significantly. Analysis of cytokine profile found that IFN-gamma released from either BALF or stimulated splenocytes increased markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4 was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.
authors.affiliation: Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. zch8@hotmail.com
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhou
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Kang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry; D001992:Bronchoalveolar Lavage Fluid; D005260:Female; D007107:Immune System; D007073:Immunoglobulin E / Q000737:chemistry; D007371:Interferon-gamma / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D008925:Mites; D008954:Models, Biological; D012220:Rhinitis / Q000209:etiology* / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism / Q000502:physiology*
chemical_list: D039741:Antigens, Dermatophagoides; D018793:Interleukin-13; D008024:Ligands; D051195:Toll-Like Receptor 2; D007073:Immunoglobulin E; D007371:Interferon-gamma