title: Farnesyl diphosphate synthase localizes to the cytoplasm of Trypanosoma cruzi and T. brucei.
abstract: The farnesyl diphosphate synthase (FPPS) has previously been characterized in trypanosomes as an essential enzyme for their survival and as the target for bisphosphonates, drugs that are effective both in vitro and in vivo against these parasites. Enzymes from the isoprenoid pathway have been assigned to different compartments in eukaryotes, including trypanosomatids. We here report that FPPS localizes to the cytoplasm of both Trypanosoma cruzi and T. brucei, and is not present in other organelles such as the mitochondria and glycosomes.
authors.affiliation: Department of Cell and Molecular Biology (CMB), Karolinska Institutet, Berzelius väg 35, 171 77 Stockholm, Sweden. marcela.ferella@ki.se
authors.forename: Marcela
authors.initials: M
authors.lastname: Ferella
authors.forename: Zhu-Hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Björn
authors.initials: B
authors.lastname: Andersson
authors.forename: Roberto
authors.initials: R
authors.lastname: Docampo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002463:Cell Membrane Permeability / Q000187:drug effects; D003593:Cytoplasm / Q000201:enzymology; D004072:Digitonin / Q000494:pharmacology; D051228:Geranyltranstransferase / Q000378:metabolism*; D007202:Indicators and Reagents / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000648:ultrastructure; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000648:ultrastructure
chemical_list: D007202:Indicators and Reagents; D051228:Geranyltranstransferase; D004072:Digitonin