Preparation of hydroxyapatite spheres with an internal cavity as a scaffold for hard tissue regeneration.
Microparticulates are currently regarded as a useful matrix for the delivery of bioactive molecules and tissue cells. Herein, hydroxyapatite (HA) spherical microparticulates with an internal cavity were produced using an oil-in-water emulsion technique. The HA slurry in the organic solvent was formulated into spherical particles in a water bath containing a surfactant. Rapid evaporation of the solvent helped create a cavity within the microparticulates. The microparticulates were heat-treated at 1,200 degrees C to produce bioactive HA particles with a mean size of approximately 363 microm. Osteoblastic cells were observed to spread and grow favorably over the surface as well as within the cavity of the microparticulates. The currently developed HA microparticulates with an internal cavity are considered to be useful as a scaffolding matrix for bone tissue engineering and direct filling skeletal defects.
Lee
Hae-Hyoung
HH
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea.
Hong
Seok-Jung
SJ
Kim
Chul-Hwan
CH
Kim
Eun-Cheol
EC
Jang
Jun-Hyeog
JH
Shin
Hong-In
HI
Kim
Hae-Won
HW
D016475:3T3 Cells; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry; D001842:Bone and Bones / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008863:Microspheres; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D012038:Regeneration; D012997:Solvents / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D014867:Water / Q000737:chemistry
D018786:Bone Substitutes; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D017886:Durapatite