Elioraea tepidiphila gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria.
A bacterial isolate, strain TU-7T, with an optimum growth temperature of about 45-50 degrees C and an optimum pH for growth between 8.0 and 8.5, was recovered from a hot spring in the Furnas area of the island of São Miguel in the Azores. The new isolate is non-pigmented, facultatively chemolithoorganotrophic, strictly aerobic and catalase- and oxidase-positive. The organism oxidized thiosulfate to sulfate with enhancement of growth. This organism assimilated organic acids but did not assimilate carbohydrates or polyols. 16S rRNA gene sequence analysis placed strain TU-7T within the radiation of the Alphaproteobacteria as a deep branch of the family Acetobacteriaceae. The major fatty acids of strain TU-7T are 18 : 1 omega 7c, 18 : 0, 19 : 0 cyclo omega 8c and an aliphatic chain with an equivalent chain-length of 20.195, identified as 19 : 0 2-OH cyclo omega 8 (Delta 11 : 12). Ubiquinone 10 is the major respiratory quinone and the major polar lipids are phosphatidylcholine, phosphatidylethanolamine, diphosphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. Bacteriochlorophyll a and puf genes were not detected. Based on 16S rRNA gene sequence analysis and physiological and biochemical characteristics, we describe a novel species of a new genus represented by strain TU-7T, for which we propose the name Elioraea tepidiphila gen. nov., sp. nov. The type strain of Elioraea tepidiphila is strain TU-7T (=DSM 17972T =CIP 109115T).
Albuquerque
Luciana
L
Department of Zoology and Center for Neurosciences and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
Rainey
Fred A
FA
Nobre
M Fernanda
MF
da Costa
Milton S
MS
D016947:Acetobacteraceae / Q000145:classification / Q000235:genetics; D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S; D013045:Species Specificity; D013696:Temperature; D009626:Terminology as Topic; D014451:Ubiquinone / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2