Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.
We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.
Sanders
Melinda E
ME
Department of Pathology, Vanderbilt University, Nashville, Tennessee 37232, USA. melinda.sanders@vanderbilt.edu
Dias
Eduardo C
EC
Xu
Baogang J
BJ
Mobley
James A
JA
Billheimer
Dean
D
Roder
Heinrich
H
Grigorieva
Julia
J
Dowsett
Mitchell
M
Arteaga
Carlos L
CL
Caprioli
Richard M
RM
D000328:Adult; D000368:Aged; D000465:Algorithms; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D040501:Calgranulin A / Q000032:analysis; D018797:Cell Cycle Proteins / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D011960:Receptors, Estrogen / Q000032:analysis; D015203:Reproducibility of Results; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D025801:Ubiquitin / Q000032:analysis
D014408:Biomarkers, Tumor; D040501:Calgranulin A; D018797:Cell Cycle Proteins; D011960:Receptors, Estrogen; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D025801:Ubiquitin; C055351:S100A6 protein, human