Rapid isolation of host-independent Bdellovibrio bacteriovorus.
A new method of isolating host-independent Bdellovibrio bacteriovorus has been developed. Filtered suspensions of host-dependent cells are dropped in small volumes onto 0.2 microm membranes laid on rich media agar. Significant growth is observed within 1-2 days; these cells were confirmed to be B. bacteriovorus using microscopic observations and PCR.
Ferguson
Megan A
MA
Department of Chemistry, SUNY New Paltz, 1 Hawk Drive, New Paltz, NY, 12561, USA.
Schmitt
Jaclyn L
JL
Sindhurakar
Anil R
AR
Volle
Catherine B
CB
Nuñez
Megan E
ME
Spain
Eileen M
EM
D001431:Bacteriological Techniques / Q000379:methods*; D001501:Bdellovibrio / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D008853:Microscopy; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial