Selective human serum albumin sensor from the screening of a fluorescent rosamine library.
A fluorescent dye library approach for the development of a bioanalyte sensor was sought. The screening of a rosamine dye library against diverse macromolecules led to the discovery of a highly sensitive human serum albumin binder, G13, with approximately 36-fold fluorescence intensity change. G13 showed a highly selective response to HSA over other macromolecules including albumins from other species. The potential use of G13 for the detection of HSA in biofluids is described.
Ahn
Young-Hoon
YH
Department of Chemistry, New York University, New York, New York 10003, USA.
Lee
Jun-Seok
JS
Chang
Young-Tae
YT
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D020650:Combinatorial Chemistry Techniques; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D054852:Small Molecule Libraries / Q000737:chemistry*; D013045:Species Specificity; D013237:Stereoisomerism
D005456:Fluorescent Dyes; D012709:Serum Albumin; D054852:Small Molecule Libraries; D012710:Serum Albumin, Bovine