Enhanced biomass and gamma-linolenic acid production of mutant strain Arthrospira platensis.
A mutant of Arthrospira platensis PCC 9108, strain M9108, obtained by mutagenesis with UV treatment, was able to mixotrophically grow in an SOT medium containing 40 g of glucose/l. The biomass and specific growth rate of strain M9108 (4.10 g/l and 0.70/d) were 1.9-fold and 1.4-fold higher, respectively, than those of the wild type (2.21 g/l and 0.58/d) under mixotrophic culture condition. In addition, when compared with the wild type, the content of gamma- linolenic acid (GLA) in the mutant was increased when glucose concentration was increased. Compared with the wild type, the GLA content of the mutant was 2-fold higher in autotrophic culture and about 3-fold higher in mixotrophic culture. Thus, the mutant appears to possess more efficient facility to assimilate and metabolize glucose and to produce more GLA than its wild-type strain.
Choi
Gang-Guk
GG
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea.
Bae
Myong-Sook
MS
Ahn
Chi-Yong
CY
Oh
Hee-Mock
HM
D018533:Biomass; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D009154:Mutation; D014466:Ultraviolet Rays; D017965:gamma-Linolenic Acid / Q000378:metabolism*
D005227:Fatty Acids; D008320:Maltose; D017965:gamma-Linolenic Acid; D005947:Glucose