title: Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1.
abstract: Genetic heterogeneity of Turkish ruminant pestiviruses was investigated by phylogenetic analysis of complete N(pro) encoding nucleotide sequences. A total of 30 virus isolates obtained from 15 provinces around the country between 1997 and 2005 were included in the phylogenetic analysis. Virus isolates mostly originated from cattle with one isolate from sheep. The bovine isolates all belonged to BVDV-1, the sheep isolate to BVDV-2. Fifteen isolates formed a new subgroup within BVDV-1, tentatively named BVDV-1l. The remaining bovine isolates were typed as BVDV-1a (n=4), BVDV-1b (n=4), BVDV-1d (n=3), BVDV-1f (n=2) and BVDV-1h (n=1). The isolates allocated to BVDV-1l originated from various geographical regions in different years. There was no correlation between genetic grouping and locations where isolates were obtained. Viruses originating from one farm in most cases belonged to the same subgroup (n=5). This study indicates that the newly detected subgroup BVDV-1l is predominant and widespread in Turkey. Moreover, an ovine virus isolate was identified as the first member of BVDV-2 reported in Turkey. A serological survey using samples from western Turkey indicated that BVDV-2 is also present in cattle.
authors.affiliation: Uludağ University, Faculty of Veterinary Medicine, Department of Microbiology, Virology, Görükle, Bursa, Turkey. kyesilbag@uludag.edu.tr
authors.forename: Yeşilbağ
authors.initials: Y
authors.lastname: Kadir
authors.forename: Förster
authors.initials: F
authors.lastname: Christine
authors.forename: Bank-Wolf
authors.initials: BW
authors.lastname: Barbara
authors.forename: Yilmaz
authors.initials: Y
authors.lastname: Zeki
authors.forename: Alkan
authors.initials: A
authors.lastname: Feray
authors.forename: Ozkul
authors.initials: O
authors.lastname: Aykut
authors.forename: Burgu
authors.initials: B
authors.lastname: Ibrahim
authors.forename: Rosales
authors.initials: R
authors.lastname: Sibilina Cedillo
authors.forename: Thiel
authors.initials: T
authors.lastname: Heinz-Jürgen
authors.forename: König
authors.initials: K
authors.lastname: Matthias
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology*; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification* / Q000235:genetics*; D030242:Diarrhea Virus 2, Bovine Viral / Q000302:isolation & purification; D014644:Genetic Variation; D010802:Phylogeny; D014421:Turkey / Q000453:epidemiology