title: Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.
abstract: We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the m/ z features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.
authors.affiliation: Department of Pathology, Vanderbilt University, Nashville, Tennessee 37232, USA. melinda.sanders@vanderbilt.edu
authors.forename: Melinda E
authors.initials: ME
authors.lastname: Sanders
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Dias
authors.forename: Baogang J
authors.initials: BJ
authors.lastname: Xu
authors.forename: James A
authors.initials: JA
authors.lastname: Mobley
authors.forename: Dean
authors.initials: D
authors.lastname: Billheimer
authors.forename: Heinrich
authors.initials: H
authors.lastname: Roder
authors.forename: Julia
authors.initials: J
authors.lastname: Grigorieva
authors.forename: Mitchell
authors.initials: M
authors.lastname: Dowsett
authors.forename: Carlos L
authors.initials: CL
authors.lastname: Arteaga
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D040501:Calgranulin A / Q000032:analysis; D018797:Cell Cycle Proteins / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D011960:Receptors, Estrogen / Q000032:analysis; D015203:Reproducibility of Results; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000032:analysis; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D025801:Ubiquitin / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D040501:Calgranulin A; D018797:Cell Cycle Proteins; D011960:Receptors, Estrogen; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D025801:Ubiquitin; C055351:S100A6 protein, human