title: [Usefulness of cytokines as surgical aggression markers in the ischemia-reperfusion syndrome and post transplant renal function in an experimental model of laparoscopic vs. open renal autotransplantation].
abstract: OBJECTIVE
  To analyze the modifications induced by laparoscopic and open nephrectomies in living donor transplantation on cytokines, to evaluate operative trauma of different surgical techniques and the influence on ischemia/reperfusion syndrome and renal function.
  
  
  METHODS
  Thirty pigs underwent left nephrectomy, 15 by laparoscopy and 15 by open approach in an experimental autotransplantation model.
  
  
  RESULTS
  Serum level of IL-2, IL-6, IL-10 and tumor necrosis factor (TNF) were lower during laparoscopic than open nephrectomy: 6.8 +/- 0.6 vs 13.9 +/- 1.1 pg/ml for IL-2, 46.2 +/- 2.3 vs 84.4 +/- 2.5 pg/ml for IL-6, 26.1 +/- 2.4 vs 92.8 +/- 12.6 pg/ml for IL-10, and 17.6 +/- 2.1 vs 38.5 +/- 4.8 pg/ml for TNF. There was no association between renal blood flow (RBF) and cytokines levels during nephrectomy: IL-2 (p = 0.498), IL-6 (p = 0.117), IL-10 (p = 0.081) y TNF (p = 0.644). However, there was correlation between IL-10 and the decrease of RBF after transplantation: (R2 0.48; p = 0.02). Initial serum creatinine levels were correlated with RBF and IL-2 levels during nephrectomy (R = 0.831, R2 = 0.691, p = 0.025), and postransplantation RBF (R = 0.784, R2 = 0.614, p < 0.0001). Seventh day creatinine levels were correlated with postransplantation RBF (R = 0.537, R2 = 0.289, p = 0.002) and IL-2 levels during nephrectomy (R = 0.685, R2 = 0.469, p = 0.015).
  
  
  CONCLUSIONS
  Cytokine levels were higher during the open approach than laparoscopic procedure. High levels of RBF during nephrectomy and transplantation improve early graft function while low levels of RBF and high levels ol IL-2 during nephrectomy induce delayed graft function.
authors.affiliation: Servicio de Urología 1, Hospital de Fuenlabrada, Fuenlabrada, Madrid, España. analinaresquevedo@hotmail.com
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Linares Quevedo
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Burgos Revilla
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Villafruela Sanz
authors.forename: Javier
authors.initials: J
authors.lastname: Zamora Romero
authors.forename: Julio
authors.initials: J
authors.lastname: Pascual Santos
authors.forename: Roberto
authors.initials: R
authors.lastname: Marcén Letosa
authors.forename: Begoña
authors.initials: B
authors.lastname: Cuevas Sánchez
authors.forename: Javier
authors.initials: J
authors.lastname: Sáenz Medina
authors.forename: Carlos
authors.initials: C
authors.lastname: Correa Gorospe
mesh_terms: D000818:Animals; D016207:Cytokines / Q000097:blood*; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000379:methods*; D010535:Laparoscopy; D015427:Reperfusion Injury / Q000097:blood* / Q000209:etiology*; D013552:Swine
chemical_list: D016207:Cytokines