Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals.
We describe a protocol for imaging bacterial luciferase (Lux)-expressing bacteria in small living animals. In this protocol, light emitted by Lux-expressing bacteria is detected and monitored by a cooled charge-coupled device detector. When these bacteria are administered to animals, it provides a potentially valuable approach to generate sensitive whole-body images with extremely low background. This imaging technology should enable the real-time monitoring of bacterial migration into both primary and metastatic tumors in several different mouse tumor models at a strong quantification power.
Min
Jung-Joon
JJ
Laboratory of In Vivo Molecular Imaging, Department of Nuclear Medicine, Chonnam National University Medical School, 5 Hak1 dong, Dong-gu, Gwangju 501-746, Republic of Korea. jjmin@jnu.ac.kr
Nguyen
Vu H
VH
Kim
Hyun-Ju
HJ
Hong
Yeongjin
Y
Choy
Hyon E
HE
D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D005456:Fluorescent Dyes; D005818:Genetic Engineering; D049449:Luminescence; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*
D005456:Fluorescent Dyes