Photolabile glutamate protecting group with high one- and two-photon uncaging efficiencies.
A pi-extended [2-(2-nitrophenyl)propoxy]carbonyl (NPPOC) derivative has been prepared as an efficient UV and near-IR photolabile protecting group for glutamate. This glutamate cage compound exhibits efficient photorelease upon one-photon excitation (epsilonPhi=990 M(-1) cm(-1) at 315 nm). In addition, it also shows efficient photorelease in activation of glutamate receptors in electrophysiological recordings. Combined with a high two-photon uncaging cross-section (deltaPhi=0.45 GM at 800 nm), its overall properties make this new cage-3-(2-propyl)-4'-methoxy-4-nitrobiphenyl (PMNB)-for glutamate a very promising tool for two-photon neuronal studies.
Gug
Sylvestre
S
Institut Gilbert Laustriat, Pharmacologie et Physicochimie, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch, France.
Charon
Sébastien
S
Specht
Alexandre
A
Alarcon
Karine
K
Ogden
David
D
Zietz
Burkhard
B
Léonard
Jérémie
J
Haacke
Stefan
S
Bolze
Frédéric
F
Nicoud
Jean-François
JF
Goeldner
Maurice
M
D004594:Electrophysiology; D018698:Glutamic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D006624:Hippocampus / Q000378:metabolism; D015394:Molecular Structure; D018408:Patch-Clamp Techniques; D010777:Photochemistry; D017785:Photons; D013053:Spectrophotometry; D046509:Tissue Culture Techniques
D018698:Glutamic Acid