Enhanced folate binding of cultured fibroblasts from Alzheimer's disease patients.
We compared the levels of serum folate from Alzheimer's disease (AD) patients and from age-matched healthy subjects and used primary cultures of fibroblasts, obtained from the two groups, to assess possible differences in their ability to bind folate. The results show that the levels of circulating folate are significantly (p<0.01; n=30) lower in AD patients than in controls (4.91+/-2.44 and 7.56+/-2.5 ng/mL, respectively). Moreover, the folate binding of AD fibroblasts is significantly (p<0.01; n=8) higher (2-4-fold) with respect to controls. RT-PCR experiments suggest that the higher folate binding could be due to an enhanced expression in AD fibroblasts of folate receptor alpha.
Cazzaniga
Emanuela
E
Department of Experimental Medicine, University of Milano-Bicocca - Via Cadore 48, 20052 Monza, MI, Italy. e.cazzaniga1@campus.unimib.it
Bulbarelli
Alessandra
A
Lonati
Elena
E
Re
Francesca
F
Galimberti
Gloria
G
Gatti
Elena
E
Pitto
Marina
M
Ferrarese
Carlo
C
Masserini
Massimo
M
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D058973:Folate Receptors, GPI-Anchored; D005492:Folic Acid / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008297:Male; D008609:Mental Status Schedule; D012333:RNA, Messenger / Q000378:metabolism; D011851:Radioactivity; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D005492:Folic Acid