title: DNMT3B 579 G>T promoter polymorphism and risk of esophagus carcinoma in Chinese.
abstract: OBJECTIVE
  To investigate the relationship between 579 G>T polymorphisms in the DNMT3B gene, which is involved in de novo methylation and associated with the risk of esophagus cancer (EC) in Chinese.
  
  
  METHODS
  DNMT3B 579 G>T genotypes were determined by PCR-RFLP in 194 EC patients and 210 healthy controls matched for age and sex, who did not receive radiotherapy or chemotherapy for newly diagnosed and histopathologically confirmed EC.
  
  
  RESULTS
  In control subjects, the frequency of T/T and G/T genotypes, and T and G alleles was 81.4%, 18.1%, 90.05% and 9.55%, respectively. The distribution of genotypes and allelotypes in the EC patients was not significantly different from that in the controls. When stratified by sex and age, there was still no significant association between the risks of EC and GT and GG genotypes. This study also showed a distinct difference in the distribution of DNMT3B and single nucleotide polymorphism (SNP) between Chinese and Koreans.
  
  
  CONCLUSIONS
  DNMT3B 579 G>T polymorphism may not be a stratification marker to predict the susceptibility to EC, at least in Chinese. DNMT3B promoter SNP is diverse in ethnic populations.
authors.affiliation: Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, Southeast University, Nanjing 210009, Jiangsu Province, China. fanh@seu.edu.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Fan
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Liu
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Zhang
authors.forename: Jia-Bo
authors.initials: JB
authors.lastname: Hu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Zhu-Jiang
authors.initials: ZJ
authors.lastname: Zhao
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002277:Carcinoma / Q000175:diagnosis* / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000502:physiology*; D004938:Esophageal Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B