Integrated Raman- and angular-scattering microscopy.
A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10 mum wide. The elastic-scattering signal was analyzed by generalized Lorenz-Mie theory, representing what we believe to be the first experimental validation of the theory's prediction of angular backscatter from single spheres. The microscope system exhibits 3 nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned.
Smith
Zachary J
ZJ
The Institute of Optics, University of Rochester, Rochester, NY 14627, USA.
Berger
Andrew J
AJ
D004548:Elasticity; D004867:Equipment Design; D007834:Lasers; D008027:Light; D008853:Microscopy; D008962:Models, Theoretical; D055095:Optics and Photonics; D009991:Oscillometry; D011137:Polystyrenes / Q000737:chemistry; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
D011137:Polystyrenes