title: [The effects of heroin on intracellular free Ca2+ of rat myocardium].
abstract: OBJECTIVE
  To observe the effects of heroin on intracellular free Ca2+ in rat myocardium.
  
  
  METHODS
  The effects of heroin on intracellular free Ca2+ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2+ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope.
  
  
  RESULTS
  Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2+ concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.e., Ca2+ concentration) leading to [Ca2+]i peak.
  
  
  CONCLUSIONS
  Heroin could affect concentrations of [Ca2+]i in myocardium and its dosage related effect needs further investigation.
authors.affiliation: Department of Forensic Medicine, Medical College of Sun Yat-sen University, Guangzhou, China. lxsa200@yahoo.com.cn
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Liu
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Wu
authors.forename: Zi-Rui
authors.initials: ZR
authors.lastname: Hao
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Liu
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Li
authors.forename: Yu-Chuan
authors.initials: YC
authors.lastname: Chen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D003932:Heroin / Q000008:administration & dosage / Q000494:pharmacology*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003932:Heroin; D002118:Calcium