[Effects of histone acetylation on differentiation of mesenchymal stem cells].
OBJECTIVE
To explore the effects of histone acetylation modification on the regulation of mesenchymal stem cells (MSCs) differentiation into cardiomyocytes in myocardium microenvironment, by detecting the acetylase activity, expression of histone acetylation gene Gcn5 and myocardial gene GATA4 in myocardium transplanted by MSCs transfected with plasmid ZJ3.


METHODS
Extracted the plasmid containing short hairpin RNA (shRNA) against Gcn5 gene (ZJ3) and transfected it into MSCs. After 24 hours, the MSCs were transplanted into the rat myocardium tissues and the acetylase activity, the expression of acetylation gene and myocardium development gene were detected after two weeks.


RESULTS
The acetylase activity in the experimental group was significantly lower than all control groups; Gcn5 and GATA4 expression in myocardium of experimental group had significant reduction compared with the control groups.


CONCLUSIONS
Inhibition of histone acetylation in the MSC cells can inhibit the transcription process of specialized myocardium in the myocardium microenvironment. The result establishes foundation for researches of histone acetylation and mechanisms of MSCs differentiation.
Li
Li
L
Division of Cardiology, Children's Hospital, Chongqing Medical University, Chongqing, 400014 P. R. China.
Zhu
Jing
J
Tian
Jie
J
D000107:Acetylation; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D051548:Histone Acetyltransferases / Q000235:genetics; D006657:Histones / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley
D006657:Histones; D051548:Histone Acetyltransferases