High expression of osteoglycin decreases the metastatic capability of mouse hepatocarcinoma Hca-F cells to lymph nodes.
Osteoglycin, one of the matrix molecules, belongs to the small leucine-rich proteoglycan gene family and might play important roles in cell growth and differentiation and in pathological processes such as fibrosis and cancer growth. In this study, a eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into mouse hepatocarcinoma Hca-F cells to evaluate the contribution of osteoglycin to the malignant behavior of Hca-F. It was found that Hca-F cells transfected with pIRESpuro3 osteoglycin(+) showed significantly decreased potential for both migration and invasion. Furthermore, Hca-F cells transfected with osteoglycin showed decreased metastatic potential to peripheral lymph nodes. However, proliferation potential and adhesive capacity of Hca-F cells to different protein substrates were not influenced by osteoglycin transfection. In summary, high expression of osteoglycin decreases the metastatic capability of Hca-F to lymph nodes.
Cui
Xiaonan
X
Department of Oncology, the First Affiliate Hospital of Dalian Medical University, Dalian 116011, China.
Song
Bo
B
Hou
Li
L
Wei
Zhiyi
Z
Tang
Jianwu
J
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000556:secondary*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary*; D008198:Lymph Nodes / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015854:Up-Regulation