title: Multiplex bead analysis of vitreous and serum concentrations of inflammatory and proangiogenic factors in diabetic patients.
abstract: OBJECTIVE
  To investigate the role of inflammatory and angiogenic factors in the pathogenesis of diabetic retinopathy, we determined, in diabetic patients and controls, vitreous and serum concentrations of interferon-induced protein (IP)-10, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, regulated upon activation, normal T-expressed and secreted (RANTES), and vascular endothelial growth factor (VEGF).
  
  
  METHODS
  We recruited 36 probands with type 2 diabetes mellitus (15 noninsulin-dependent and 21 insulin-dependent) and 69 normal controls. Using Cytometric Bead Array Technology, we measured vitreous and serum concentrations of IP-10, MCP-1, MIP-1alpha, MIP-1beta, RANTES, and VEGF.
  
  
  RESULTS
  In diabetic patients the mean vitreous levels of IP-10, MCP-1 and VEGF were significantly higher compared normal controls. [IP-10 (pg/mL) 254.84 +/-311.67 versus 78.90 +/- 67.94 (p<0.001); MCP-1 (pg/mL) 1127.14 +/- 738.91 versus 700.80 +/-419.21 (p=0.002); VEGF (pg/mL) 954.98 +/- 2315.09 versus 37.90 +/- 28.51(p<0.001)]. Vitreous levels of VEGF correlated with vitreous levels of both IP-10 and MCP-1 (p<0.05). MIP-1beta, RANTES, and VEGF mean serum levels were significantly raised in diabetic probands while IP-10, MCP-1, and MIP-1alpha serum levels showed no significant elevation compared to controls [IP-10 (pg/mL) 346.20 +/- 287.36 versus 328.74 +/-352.35 (p=0.88); MCP-1(pg/mL) 133.10 +/- 89.10 versus 141.47 +/- 222.15 (p=0.50); MIP-1beta (pg/mL) 184.40 +/- 100.20 versus 139.56 +/- 151.38 (p=0.003); RANTES (pg/mL) 51336.23 +/- 19940.31 versus 33629.2 +/- 33301.0 (p=0.002); VEGF (pg/mL) 304.88 +/- 257.52 versus 154.45 +/- 114.78 (p<0.001)].
  
  
  CONCLUSIONS
  Our results suggest that in diabetics, there is an upregulation of IP-10, MCP-1, and VEGF in the vitreous and an upregulation of MIP-1beta, RANTES, and VEGF in the serum. These findings support the concept of an angiogenic and inflammatory element in the development of diabetic retinopathy.
authors.affiliation: Department of Ophthalmology, Medical University of Graz, Graz, Austria. richardmaier@chello.at
authors.forename: Richard
authors.initials: R
authors.lastname: Maier
authors.forename: Martin
authors.initials: M
authors.lastname: Weger
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Haller-Schober
authors.forename: Yosuf
authors.initials: Y
authors.lastname: El-Shabrawi
authors.forename: Andreas
authors.initials: A
authors.lastname: Wedrich
authors.forename: Anna
authors.initials: A
authors.lastname: Theisl
authors.forename: Reingard
authors.initials: R
authors.lastname: Aigner
authors.forename: Alfred
authors.initials: A
authors.lastname: Barth
authors.forename: Anton
authors.initials: A
authors.lastname: Haas
mesh_terms: D000368:Aged; D043925:Angiogenesis Inducing Agents / Q000097:blood / Q000378:metabolism*; D018932:Chemokine CCL2 / Q000378:metabolism; D054407:Chemokine CCL4 / Q000097:blood; D018946:Chemokine CCL5 / Q000097:blood; D054357:Chemokine CXCL10 / Q000378:metabolism; D003920:Diabetes Mellitus / Q000097:blood / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism; D014822:Vitreous Body / Q000378:metabolism*
chemical_list: D043925:Angiogenesis Inducing Agents; C111835:CCL2 protein, human; C114753:CXCL10 protein, human; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054357:Chemokine CXCL10; D018836:Inflammation Mediators; D042461:Vascular Endothelial Growth Factor A