Identification of major constituents in the traditional Chinese medicine "QI-SHEN-YI-QI" dropping pill by high-performance liquid chromatography coupled with diode array detection-electrospray ionization tandem mass spectrometry.
An HPLC-DAD-ESI-MS(n) method was developed for simultaneous analysis of major chemical constituents in "QI-SHEN-YI-QI" dropping pill, a traditional Chinese medicine (TCM) widely used for treating cardiovascular diseases. The chromatographic separation was performed on an intertsil ODS-3 C(18) column (4.6 mm x 250 mm, 5 microm), whilst water with 0.05% acetic acid and acetonitrile were used as mobile phase. On the basis of the characteristic UV absorption profile, the information of molecular weight, and structure provided by ESI-MS(n), 31 constituents derived from Astragalus membranaceus, Radix Salviae Miltiorrhizae, and Panax notoginseng, were detected and 20 of them were identified in this study. The proposed method contributes to the quality control of "QI-SHEN-YI-QI" dropping pill.
Yunfei
Li
L
Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou, PR China.
Haibin
Qu
Q
Yiyu
Cheng
C
D002317:Cardiovascular Agents / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D008970:Molecular Weight; D011786:Quality Control; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D002317:Cardiovascular Agents; D004365:Drugs, Chinese Herbal