Lipid rafts association of synaptotagmin I on synaptic vesicles.
We confirmed the raft association of synaptotagmin I (syt I) in synaptic vesicles by sucrose density gradient centrifugation, cholesterol depletion, and temperature dependence, and Ca2+ was found to positively regulate this association. Furthermore, using syt I mutants we found that the transmembrane domain (TMD) of syt I plays an important role in localizing syt I into the lipid rafts of synaptic vesicles, and the raft association of the TMD can be regulated by its phosphorylation status.
Lv
Ji-Hua
JH
Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China.
He
Li
L
Sui
Sen-Fang
SF
D000818:Animals; D002118:Calcium / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry*; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D051381:Rats; D017207:Rats, Sprague-Dawley; D013572:Synaptic Vesicles / Q000737:chemistry*; D050863:Synaptotagmin I / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
D050863:Synaptotagmin I; D002118:Calcium