title: Hydrolysis of isoflavone glucosides in soymilk fermented with single or mixed cultures of Lactobacillus paraplantarum KM, Weissella sp. 33, and Enterococcus faecium 35 isolated from humans.
abstract: Lactobacillus paraplantarum KM (Lp), Weissella sp. 33 (Ws), and Enterococcus faecium 35 (Ef) were used in single (Lp, Ws, Ef) or mixed cultures (Lp+Ws, Lp+Ef, Ws+Ef) for soymilk fermentation (37 degrees C, 12 h). After 12 h, the cell numbers, pH, and TA of soymilk were 7.4x108 -6.0x109 CFU/ ml, 3.8-4.5, and 0.59-0.70%, respectively. Changes in the contents of glycitin and genistin in soymilk fermented with Ef were not significant (p<0.05). The contents of isoflavone glucosides in soymilk fermented with the other cultures decreased significantly with an increase of aglycone contents (p<0.05). It corresponded well with a sharp increase in beta- glucosidase activity during fermentation. About 92-100% of the daidzin and 98-100% of the genistin in soymilk were converted to corresponding aglycones by Lp, Ws, or Lp+Ef within 12 h.
authors.affiliation: Department of Food Science and Technology, Sunchon National University, Sunchon, Korea.
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Chun
authors.forename: Woo Ju
authors.initials: WJ
authors.lastname: Jeong
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Lim
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Induck
authors.initials: I
authors.lastname: Choi
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D016984:Enterococcus faecium / Q000254:growth & development / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D005960:Glucosides / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007529:Isoflavones / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism; D041942:Soy Milk / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005960:Glucosides; D007529:Isoflavones; D019344:Lactic Acid; D001617:beta-Glucosidase