title: Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae).
abstract: A reovirus was isolated from Operophtera brumata (ObRV) and its parasitoid wasp Phobocampe tempestiva. Each of the 10 dsRNA genome segments of ObRV was sequenced and shown to contain a single open reading frame (ORF). Conserved motifs ([+ve] 5'-AAATAAA ...(G)/(T)AGGTT-3') were found at the termini of each segment, with the exception of Seg-6 and Seg-8, where the 5' termini were 5'-AACAAA...-3'. The putative proteins encoded by each segment were compared with those of other members of the family Reoviridae. Phylogenetic comparisons to published sequences for the RNA-dependent RNA polymerase genes from other reoviruses indicated that ObRV is most closely related to members of the genus Cypovirus. However, unlike the cypoviruses, ObRV has a double-layered capsid structure. When the protein encoded by ObRV Seg-10 was expressed (by inserting the open reading frame into a baculovirus expression vector) no 'occlusion bodies' were observed in the recombinant baculovirus infected insect cell cultures. This suggests that unlike the cypoviruses, Seg-10 of ObRV does not contain a polyhedrin gene. Further phylogenetic comparisons also identified relationships between Seg-2 and Seg-10 of ObRV, and genes of Diadromus pulchellus Idnoreovirus 1 (DpIRV1), suggesting that ObRV represents a new species from the genus Idnoreovirus.
authors.affiliation: NERC Centre for Ecology and Hydrology, Mansfield Road, Oxford OX1 3SR, UK. rob.graham@csiro.au
authors.forename: Robert I
authors.initials: RI
authors.lastname: Graham
authors.forename: Shujing
authors.initials: S
authors.lastname: Rao
authors.forename: Steven M
authors.initials: SM
authors.lastname: Sait
authors.forename: Houssam
authors.initials: H
authors.lastname: Attoui
authors.forename: Peter P C
authors.initials: PP
authors.lastname: Mertens
authors.forename: Rosemary S
authors.initials: RS
authors.lastname: Hails
authors.forename: Robert D
authors.initials: RD
authors.lastname: Possee
mesh_terms: D000818:Animals; D015870:Gene Expression; D016679:Genome, Viral; D007304:Insect Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016366:Open Reading Frames; D010802:Phylogeny; D012087:Reoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D017421:Sequence Analysis; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014863:Wasps / Q000821:virology*
chemical_list: D014764:Viral Proteins