title: Nuclear swelling occurs during premature senescence mediated by MAP kinases in normal human fibroblasts.
abstract: Excess thymidine induced premature senescence in normal human fibroblasts (TIG-7), with induction of typical senescence markers. Nuclear swelling, as well as cell swelling, was clearly observed in these senescent cells. Simultaneous addition of MAP kinase inhibitors, U0126, SB203580, and SP60025, effectively suppressed induction of premature senescence and senescence markers.
authors.affiliation: Kihara Institute for Biological Research, Graduate School of Integrated Science, Yokohama City University, 641-12 Maioka-cho, Yokohama 244-0813, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Risa
authors.initials: R
authors.lastname: Sakemura
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kumagai
authors.forename: Emi
authors.initials: E
authors.lastname: Sumikawa
authors.forename: Michihiko
authors.initials: M
authors.lastname: Fujii
authors.forename: Dai
authors.initials: D
authors.lastname: Ayusawa
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D053145:Cell Nucleus Size; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016922:Cellular Senescence; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013936:Thymidine / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D020928:Mitogen-Activated Protein Kinases; D013936:Thymidine