Microglial Fc receptors mediate physiological changes resulting from antibody cross-linking of myelin oligodendrocyte glycoprotein.
Antibodies to myelin oligodendrocyte glycoprotein (MOG) have been implicated in Multiple Sclerosis demyelination through activation of complement and/or macrophage-effector processes. We presented a novel mechanism, whereby MOG on oligodendrocytes, when cross-linked with anti-MOG and secondary antibody resulted in its repartitioning into lipid rafts, and changes in protein phosphorylation and morphology. Here, we show that similar events occur when anti-MOG is cross-linked with Fc receptors (FcRs) present on microglia but not with complement. These results indicate that FcRs are endogenous antigen/antibody cross-linkers in vitro, suggesting that FcRs could be physiologically relevant in vivo and possible targets for therapy in Multiple Sclerosis.
Marta
Cecilia B
CB
Department of Neuroscience, University of Connecticut Medical School, Farmington, CT 06030-3401, United States.
Bansal
Rashmi
R
Pfeiffer
Steven E
SE
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D016923:Cell Death; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism* / Q000494:pharmacology; D004676:Myelin Basic Protein / Q000378:metabolism; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D009836:Oligodendroglia / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011961:Receptors, Fc / Q000502:physiology*; D013997:Time Factors; D047392:beta-Cyclodextrins / Q000494:pharmacology
D015703:Antigens, CD; D003432:Cross-Linking Reagents; D007074:Immunoglobulin G; C571328:Mog protein, rat; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D011961:Receptors, Fc; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin