Expression of toll-like receptors in the Fusarium solani infected cornea.
OBJECTIVE
To investigate the expression of toll-like receptors (TLRs) in the human cornea with Fusarium solani (F. solani) keratitis.


METHODS
Five human corneas with F. solani keratitis and 5 healthy human corneas were evaluated for TLR1-10 mRNA by reverse transcribed polymerase chain reaction (RT-PCR) and relative real-time PCR. The proteins of TLR2, 4, and 9 were also compared by Western blot. The mean times between these corneas were collected, and the onset of treatment to surgery was 34.4 +/- 12.4 days, ranging from 15 to 47 days. The data were analyzed with SPSS15.0.


RESULTS
TLR1-10 mRNA was expressed in both healthy and F. solani infected human corneas. The expression of TLR2, 4, 6, and 9 mRNA in the F. solani infected human corneas were upregulated. The Western blot showed the protein expression of TLR2, 4, and 9 was also upregulated in the corneas with F. solani keratitis than that of the healthy corneas.


CONCLUSIONS
TLRs are expressed diversely in the F. solani infected human cornea, and TLR2, 4, and 9 may be implicated in the pathogenesis of F. solani infection in the cornea.
Jin
Xiuming
X
Eye Center, Affiliated Second Hospital, School of Medicine, Zhejiang University, Hangzhou, PR China.
Qin
Qin
Q
Lin
Zhenyun
Z
Chen
Wei
W
Qu
Jia
J
D000328:Adult; D000368:Aged; D003315:Cornea / Q000378:metabolism* / Q000382:microbiology; D015821:Eye Infections, Fungal / Q000150:complications* / Q000378:metabolism*; D005670:Fusarium; D006801:Humans; D007634:Keratitis / Q000382:microbiology*; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D051201:Toll-Like Receptor 10 / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D012333:RNA, Messenger; C475955:TLR10 protein, human; C495345:TLR4 protein, human; C495371:TLR9 protein, human; D051201:Toll-Like Receptor 10; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D051217:Toll-Like Receptor 9; D051193:Toll-Like Receptors