[Study on molecular epidemiology of people infected with human immunodeficiency virus-1 in Hubei province].
OBJECTIVE
To study the distribution of human immunodeficiency virus-1 (HIV-1) genotypes in Hubei province.


METHODS
Epidemiological survey was carried out to HIV-1 carriers who were identified in Hubei province. HIV-1 env V3-V4, gag P17/24 and the first exon of tat region were amplified by nested-polymerase chain reaction(nPCR) .The sequences were determined, and phylogenetic analyses were then performed.


RESULTS
4 HIV-1 strains or circulating recombinant forms (CRFs) were identified in Hubei province with subtype B' the predominant which covered 5 kinds of populations including former blood donors, blood receivers, spouses of the infected people, sex workers and their clients, homosexuals, mainly distributed in the areas with many former blood donors. CRF08-BC and CRF01-AE were found distributed in economically more developed cities or southern area of the province, and the major transmission routes was through sexual contact. Only 1 patient, an injecting drug user, was identified having subtype C.


CONCLUSIONS
Subtype B' was the main epidemic subtypes in Hubei province while CRF08-BC, CRF01-AE and subtype C were also circulating in the province, indicating the transmission of the disease might to become more complex.
Chu
Xiao-gang
XG
Hubei Provincal Center for Disease Control and Prevention, Wuhan 430079, China.
Zhang
Xian-feng
XF
Zhan
Fa-xian
FX
Tang
Heng
H
Chen
Hui-ping
HP
Peng
Ting-hai
TH
Gong
Zuo-jiong
ZJ
D002681:China / Q000453:epidemiology; D015658:HIV Infections / Q000453:epidemiology*; D015497:HIV-1 / Q000145:classification; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA
D012367:RNA, Viral