title: Adenovirus vector-mediated gene therapy using iodized oil esters for hepatocellular carcinoma in rats.
abstract: BACKGROUND
  When gene therapy is performed for malignant tumors, gene transfer efficiency and selectivity are extremely important. The usefulness of gene therapy by intraarterial injection of an adenovirus vector with iodized oil esters (IOEs) for hepatocellular carcinoma (HCC) was studied.
  
  
  METHODS
  HCC was induced in rats with diethyl nitrosamine and phenobarbital, after which either adenovirus vector expressing the herpes simplex virus thymidine kinase (AxCAHSVtk) and IOEs or AxCAHSVtk alone was injected through the hepatic artery. On postoperative days 2, 4 and 6, gancyclovir was injected into the peritoneum; blood sampling was performed on day 7.
  
  
  RESULTS
  Aspartate aminotransferase and alanine aminotransferase levels in the AxCAHSVtk with IOEs group were lower than in the AxCAHSVtk alone group (p = 0.0274, p = 0.0323). However, the survival rate was not significantly different between groups (p = 0.7122).
  
  
  CONCLUSIONS
  Intra-arterial injection of an adenovirus vector with IOEs can result in cancer-selective but not effective gene therapy for HCC.
authors.affiliation: Department of Surgery, Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan. hs0817@jikei.ac.jp
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shiba
authors.forename: Takeyuki
authors.initials: T
authors.lastname: Misawa
authors.forename: Tomonori
authors.initials: T
authors.lastname: Iida
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Okamoto
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Futagawa
authors.forename: Minori
authors.initials: M
authors.lastname: Sakurai
authors.forename: Toya
authors.initials: T
authors.lastname: Ohashi
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Eto
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Yanaga
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002645:Chickens; D015774:Ganciclovir / Q000008:administration & dosage / Q000493:pharmacokinetics; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics; D006499:Hepatic Artery; D007269:Injections, Intra-Arterial; D007459:Iodized Oil / Q000008:administration & dosage*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000628:therapy*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics; D013937:Thymidine Kinase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D007459:Iodized Oil; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D013937:Thymidine Kinase; D015774:Ganciclovir