The concentration-dependent induction of cell death by trimethyltin chloride in rat liver epithelial IAR20 cells.
In this study we intended to evaluate the cytotoxic and genotoxic effects of trimethyltin chloride (TMT), one of the most widely used organometallic compounds, on liver cells with the rat liver epithelial cell line IAR20. The results showed that TMT significantly inhibited cell growth in a concentration-dependent manner and caused an increase in DNA damage. Additionally, Hoechst 33342 staining and flow cytometry detected increases in apoptotic and necrotic cells. Western blots demonstrated that the Bcl-2 family of proteins was involved in the apoptotic process, but p53 was not concerned.
Wang
Mengmeng
M
Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Siming South Road, Xiamen City 361005, PR China.
Li
Bowen
B
Wang
Chonggang
C
Chen
Yixin
Y
Zuo
Zhenghong
Z
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D009336:Necrosis / Q000139:chemically induced; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D014298:Trimethyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D019253:Proto-Oncogene Proteins c-bcl-2; D014298:Trimethyltin Compounds; D014874:Water Pollutants, Chemical; D004247:DNA; C040533:trimethyltin chloride