title: Development and preliminary results of an endoscopic Raman probe for potential in vivo diagnosis of lung cancers.
abstract: A near-infrared Raman system was developed to collect real-time in vivo human lung spectra. The excitation light and the emission were guided to and from the tissue surface by a reusable fiber catheter passed down the instrument channel of a bronchoscope. Two-stage filtering was used to reduce laser noise, fluorescence, and Raman emissions from the fibers. A second fiber bundle guided the emission to a spectrometer where the fibers, in a round packing geometry, were spread out to form a parabolic arc that improved the signal-to-noise ratio 20-fold, facilitating real-time spectral measurements. Preliminary clinical tests show that clear and reliable Raman spectra can be obtained.
authors.affiliation: Cancer Imagin Department, British Columbia Cancer Research Centre, Vancouver, B.C., Canada.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Short
authors.forename: Stephen
authors.initials: S
authors.lastname: Lam
authors.forename: Annette
authors.initials: A
authors.lastname: McWilliams
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhao
authors.forename: Harvey
authors.initials: H
authors.lastname: Lui
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D001706:Biopsy; D020680:Bronchoscopes; D001999:Bronchoscopy / Q000379:methods; D002138:Calibration; D004724:Endoscopy / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D007834:Lasers; D008168:Lung / Q000473:pathology*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*