title: Crystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis.
abstract: Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS-DevR is a two-component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting-drop vapour-diffusion method in the presence of sodium citrate and 2-propanol as precipitants. X-ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 A on a synchrotron beamline. The crystals belong to the hexagonal space group P6(1). The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 A(3) Da(-1). The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.
authors.affiliation: School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Ha Yeon
authors.initials: HY
authors.lastname: Cho
authors.forename: Hyo Je
authors.initials: HJ
authors.lastname: Cho
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.forename: Jeong Il
authors.initials: JI
authors.lastname: Oh
authors.forename: Beom Sik
authors.initials: BS
authors.lastname: Kang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006420:Hemeproteins / Q000737:chemistry; D020102:Mycobacterium smegmatis / Q000201:enzymology*; D006656:Protamine Kinase / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D006420:Hemeproteins; C506260:DevS protein, Mycobacterium tuberculosis; D006656:Protamine Kinase