title: Amino acid sensing using aminoacyl-tRNA synthetase.
abstract: The detection of amino acids using aminoacyl-tRNA synthetases (ARSs) as the molecular recognition element was proposed, and the binding activity and specificity of ARSs were evaluated. Using this rapid and easy method, from 15 to 50 microM tyrosine could be measured specifically. The method suggested in this article could be realized without an amino acid labeling process or a large volume of organic solvents, and the time for measurement was reasonable.
authors.affiliation: Research Center for Advanced Science and Technology, The City of Hiroshima, Naka-ku, Hiroshima 730-0052, Japan. kugimiya@sentan.city.hiroshima.jp
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Kugimiya
authors.forename: Miki
authors.initials: M
authors.lastname: Morii
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohtsuki
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D013379:Substrate Specificity; D020124:Thermotoga maritima / Q000201:enzymology
chemical_list: D000596:Amino Acids; D000604:Amino Acyl-tRNA Synthetases