Sensitivity and specificity of various serological tests for the detection of Toxoplasma gondii infection in naturally infected sheep.
Comparative serological examination of 300 serum samples from sheep slaughtered in the main abattoir in Cairo, Egypt revealed a higher prevalence of toxoplasmosis (43.7%) with the modified agglutination test (MAT), followed by the enzyme linked immune-sorbant assay (ELISA) (41.7%) and the indirect fluorescent antibody test (IFAT) (37%), while the lowest prevalence was detected with the dye test (DT) (34%). When the data from the first three serological tests were compared with that of the DT test, which was used as a reference test for toxoplasmosis, MAT had the highest sensitivity (96%), followed by ELISA (90.1%) and IFAT, which demonstrated the lowest sensitivity (80.4%). Conversely, IFAT had the highest specificity (91.4%), followed by MAT (88.9%) and ELISA (85.9%).
Shaapan
R M
RM
Department of Zoonosis, National Research Center, Giza, Egypt. rmshaapan2005@yahoo.com
El-Nawawi
F A
FA
Tawfik
M A A
MA
D000003:Abattoirs; D000372:Agglutination Tests / Q000379:methods / Q000592:standards / Q000662:veterinary; D000818:Animals; D004534:Egypt / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods / Q000592:standards / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000453:epidemiology; D014122:Toxoplasma / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000453:epidemiology