Proteomic investigation of the adaptation of Lactococcus lactis to the mouse digestive tract.
Lactic acid bacteria are used on an industrial scale for the manufacturing of dairy products. It is now intended to develop novel applications of lactic acid bacteria that could be used as living vehicles for the targeting of antigens or therapeutics to the digestive mucosa. The aim of this study was to analyze the adaptations of Lactococcus lactis, a model lactic acid bacteria to the digestive tract and to identify functions required for colonization of the intestine. For this purpose, we combined gnotobiology with proteomics: axenic mice were colonized with a dairy L. lactis strain and the bacterial proteome was examined by 2-DE. As compared to cultures in broth, the proteome profile of bacteria grown in the intestine indicates the activation of metabolic pathways involved in various carbon sources assimilation and suggests the adoption of a mixed acids fermentative metabolism. We identified the product of the ywcC gene as essential for the colonization of the digestive tract and demonstrated that the corresponding gene product (YwcC) possesses a phosphogluconolactonase activity, suggesting an important role of the pentose phosphate pathway for the development of L. lactis in the digestive environment.
Roy
Karine
K
INRA, Unité de Biochimie Bactérienne, Centre de Recherches de Jouy-en-Josas, Jouy-en-Josas, France.
Meyrand
Mickaël
M
Corthier
Gérard
G
Monnet
Véronique
V
Mistou
Michel-Yves
MY
D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000382:microbiology*; D005856:Germ-Free Life; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D010427:Pentose Phosphate Pathway; D020543:Proteome / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D017931:DNA Primers; D020543:Proteome; D012333:RNA, Messenger; D002265:Carboxylic Ester Hydrolases; C022363:6-phosphogluconolactonase