Rotating versus perfusion bioreactor for the culture of engineered vascular constructs based on hyaluronic acid.
It is generally accepted that dynamic culture conditions are required for vascular tissue engineering. We compared the effects of two dynamic culture systems, a perfusion and a rotating bioreactor, using tubular constructs based on hyaluronic acid seeded with porcine aortic smooth muscle cells (SMC), that we recently showed to be adequate for the generation of vascular tissue. In perfused constructs mechanical stimulation importantly affected cell morphology, increased the incidence of cell proliferation and reduced apoptosis. However, extracellular matrix deposition, cytoskeletal organization and mechanical properties were poor. In rotated constructs cell proliferation was also higher and apoptosis lower than in static controls. Rotated constructs showed the highest ultimate stress and the lowest elastic modulus. Our data indicate that the rotating bioreactor is more efficient than the perfusion bioreactor and we then suggest that this method can be considered a valid alternative to complex bioreactor systems described in the literature.
Arrigoni
Chiara
C
Department of Biomedical Engineering, Mario Negri Institute for Pharmacological Research, Via Gavazzeni, 11, 24125 Bergamo, Italy.
Chittò
Annalisa
A
Mantero
Sara
S
Remuzzi
Andrea
A
D000818:Animals; D019149:Bioreactors; D001807:Blood Vessel Prosthesis; D001808:Blood Vessels / Q000254:growth & development*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006820:Hyaluronic Acid / Q000737:chemistry*; D040542:Mechanotransduction, Cellular / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology*; D010477:Perfusion / Q000295:instrumentation; D012399:Rotation; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*
D006820:Hyaluronic Acid