title: Natural history and histology in a rat model of laser-induced photothrombotic retinal vein occlusion.
abstract: OBJECTIVE
  To observe temporal changes in retinal physiology and histology in a rat model of laser-induced retinal vein occlusion (RVO).
  
  
  METHODS
  Ophthalmoscopy, fundus photography, and fluorescein angiography (FA) were performed following laser-induced central retinal vein occlusion (CRVO) and branch retinal vein occlusion (BRVO) immediately after laser treatment and at 3 and 6 hr and 2, 4, 7, 14, and 21 days. Retinal histology was examined at 4, 7, 14, and 21 days. Full-field electroretinogram was recorded from both eyes simultaneously at day 4.
  
  
  RESULTS
  For CRVO and BRVO, reperfusion of occluded branch veins was observed 1 to 2 days after treatment. Despite complete reperfusion of branch veins, retinal edema and hemorrhages peaked on day 4, and by day 14, treated retinas appeared pale and edematous upon ophthalmoscopy. In BRVO animals, retinal hemorrhages were limited to the vein-occluded region, although edema was more widespread and, to a limited extent, involved the untreated hemi-retina. Significant GCL cell loss was observed in both CRVO and BRVO groups after day 14. Regional analysis showed that relative GCL loss was greatest in the peripheral retina in BRVO group. Electroretinography disclosed moderate to severe functional deficits in photoreceptors, bipolar, and amacrine and ganglion cells.
  
  
  CONCLUSIONS
  Laser-induced RVO in rats results in targeted vascular occlusion that persisted for 1 to 2 days. Functional deficits were evident and significant GCL cell loss was seen, notably within peripheral retina of the BRVO model. This reproducible model provides a valuable tool for the study of the molecular events associated with retinal ischemia and cell death.
authors.affiliation: Casey Eye Institute, Oregon Health and Science University, Portland, Oregon 97239-4197, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Brad
authors.initials: B
authors.lastname: Fortune
authors.forename: La-ongsri
authors.initials: LO
authors.lastname: Atchaneeyasakul
authors.forename: Trevor
authors.initials: T
authors.lastname: McFarland
authors.forename: Kristen
authors.initials: K
authors.lastname: Mose
authors.forename: Patrick
authors.initials: P
authors.lastname: Wallace
authors.forename: Julianne
authors.initials: J
authors.lastname: Main
authors.forename: David
authors.initials: D
authors.lastname: Wilson
authors.forename: Binoy
authors.initials: B
authors.lastname: Appukuttan
authors.forename: J Timothy
authors.initials: JT
authors.lastname: Stout
mesh_terms: D000818:Animals; D002452:Cell Count; D004596:Electroretinography; D005260:Female; D005654:Fundus Oculi; D007834:Lasers; D023421:Models, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000503:physiopathology; D012165:Retinal Ganglion Cells / Q000473:pathology*; D012170:Retinal Vein Occlusion / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D013927:Thrombosis / Q000150:complications / Q000209:etiology*