title: Interdisciplinary study for the evaluation of biochemical alterations on mussel Mytilus galloprovincialis exposed to a tributyltin-polluted area.
abstract: An interdisciplinary approach was employed to monitor the concentration and the effects of butyltin compounds in mussels (Mytilus galloprovincialis). Tissues from animals exposed to a marine area (Vado Ligure harbour) with a high concentration of tributyltin (TBT) were analysed and compared with control samples. TBT concentrations were measured by gas chromatography-mass spectrometry and the protein pattern in gill tissues was studied by proteomic analysis. Several proteomic signatures associated with contaminant exposure were observed; spots that were significantly increased in all contaminated samples were identified by mass spectrometry as fragments of β-tubulin. The degradation of β-tubulin was then confirmed by western blot analysis with specific anti-β-tubulin antibody. The effects observed on mussel gills after exposure in the TBT-polluted area are discussed.
authors.affiliation: Department of Chemistry and Industrial Chemistry, University of Genoa, 16146, Genoa, Italy. magie@chimica.unige.it
authors.forename: Emanuele
authors.initials: E
authors.lastname: Magi
authors.forename: Camilla
authors.initials: C
authors.lastname: Liscio
authors.forename: Erika
authors.initials: E
authors.lastname: Pistarino
authors.forename: Barbara
authors.initials: B
authors.lastname: Santamaria
authors.forename: Marina
authors.initials: M
authors.lastname: Di Carro
authors.forename: Micaela
authors.initials: M
authors.lastname: Tiso
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: Giovanni
authors.initials: G
authors.lastname: Renzone
authors.forename: M Elisabetta
authors.initials: ME
authors.lastname: Cosulich
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D008401:Gas Chromatography-Mass Spectrometry; D005880:Gills / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014220:Trialkyltin Compounds / Q000032:analysis* / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism*
chemical_list: D014220:Trialkyltin Compounds; D014404:Tubulin; C011559:tributyltin