Increased plasma and optic nerve levels of IL-6, TNF-alpha, and MIP-2 following induction of ischemic optic neuropathy in mice.
OBJECTIVE
To investigate levels of proinflammatory cytokines in a mouse model of anterior ischemic optic neuropathy (rAION).


METHODS
AION was induced in C57/BL6 mice and levels of IL-6, TNF-alpha, and MIP-2 were measured in plasma by ELISA and in the optic nerves by RT-PCR at predetermined intervals.


RESULTS
Plasma: IL-6 levels were elevated immediately after rAION induction and decreased gradually thereafter. TNF-alpha showed an early peak on day 1 and again from day 21. MIP-2 levels were increased until day 7. Optic nerve: IL-6, TNF-alpha, and MIP-2 levels increased within a few hours and then decreased gradually. IL-6 had a second peak on day 3.


CONCLUSIONS
Proinflammatory cytokines may play a role in the pathogenesis of rAION.
Avraham
Bat Chen R
BC
The Krieger Eye Research Laboratory, Felsenstein Medical Research Center, Petach Tikva, Israel.
Dotan
Gad
G
Hasanreisoglu
Murat
M
Kramer
Michal
M
Monselise
Yehudit
Y
Cohen
Yoram
Y
Weinberger
Dov
D
Goldenberg-Cohen
Nitza
N
D000818:Animals; D054426:Chemokine CXCL2 / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009900:Optic Nerve / Q000378:metabolism*; D018917:Optic Neuropathy, Ischemic / Q000097:blood / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism*
D054426:Chemokine CXCL2; C404915:Cxcl2 protein, mouse; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha