title: Antitubercular triterpenes and phytosterols from Pandanus tectorius Soland. var. laevis.
abstract: Bioassay-guided chromatographic purification of the antitubercular chloroform extract of Pandanus tectorius Soland. var. laevis leaves afforded a new tirucallane-type triterpene, 24,24-dimethyl-5 beta-tirucall-9(11),25-dien-3-one (1), squalene and a mixture of the phytosterols stigmasterol and beta-sitosterol. Microplate Alamar Blue Assay (MABA) showed that 1 inhibited the growth of Mycobacterium tuberculosis H(37)Rv with a MIC of 64 microg/mL, while squalene and the sterol mixture have MICs of 100 and 128 microg/mL, respectively.
authors.affiliation: Research Center for the Natural Sciences, Graduate School and College of Science, University of Santo Tomas, España, 1015, Manila, Philippines.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Tan
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Takayama
authors.forename: Norio
authors.initials: N
authors.lastname: Aimi
authors.forename: Mariko
authors.initials: M
authors.lastname: Kitajima
authors.forename: Scott G
authors.initials: SG
authors.lastname: Franzblau
authors.forename: Maribel G
authors.initials: MG
authors.lastname: Nonato
mesh_terms: D000995:Antitubercular Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development; D031673:Pandanaceae / Q000737:chemistry*; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D012855:Sitosterols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013185:Squalene / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013265:Stigmasterol / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: C529812:24,24-dimethyltirucall-9(11),25-dien-3-one; D000995:Antitubercular Agents; D010840:Phytosterols; D010936:Plant Extracts; D012855:Sitosterols; D014315:Triterpenes; C025473:gamma-sitosterol; D013185:Squalene; D013265:Stigmasterol