title: Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects.
abstract: OBJECTIVE
  To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non-UC colonic microbiota in vitro.
  
  
  RESULTS
  Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non-UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short-chain fatty acids were determined. UC and non-UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate-reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0.009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0.0004). The levels of beneficial short-chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA.
  
  
  CONCLUSIONS
  The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA.
  
  
  CONCLUSIONS
  Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC.
authors.affiliation: School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, Whiteknights, Reading, UK.
authors.forename: D J S
authors.initials: DJ
authors.lastname: Mills
authors.forename: K M
authors.initials: KM
authors.lastname: Tuohy
authors.forename: J
authors.initials: J
authors.lastname: Booth
authors.forename: M
authors.initials: M
authors.lastname: Buck
authors.forename: M J C
authors.initials: MJ
authors.lastname: Crabbe
authors.forename: G R
authors.initials: GR
authors.lastname: Gibson
authors.forename: J M
authors.initials: JM
authors.lastname: Ames
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D001431:Bacteriological Techniques; D001439:Bacteroides / Q000187:drug effects / Q000502:physiology; D016022:Case-Control Studies; D002417:Cattle; D003013:Clostridium / Q000187:drug effects / Q000502:physiology; D003093:Colitis, Ulcerative / Q000382:microbiology*; D003106:Colon / Q000382:microbiology*; D015169:Colony Count, Microbial / Q000379:methods; D004044:Dietary Proteins / Q000494:pharmacology*; D004063:Digestion; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005260:Female; D005502:Food; D017127:Glycation End Products, Advanced; D006358:Hot Temperature; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D021521:Ribotyping; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism; D000093423:Glycated Serum Albumin
chemical_list: D004044:Dietary Proteins; D005232:Fatty Acids, Volatile; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D013431:Sulfates; D012710:Serum Albumin, Bovine; D000093423:Glycated Serum Albumin