CYP1A1 *2B and *4 polymorphisms are associated with lung cancer susceptibility in Mexican patients.
BACKGROUND
CYP1A1 is a gene involved in the high aryl hydrocarbon hydroxylase -inducible phenotype, which is a genetically-determined variation among individuals that has been associated with lung cancer risk. More specifically, CYP1A1 *2B and *4 polymorphisms have been associated with high susceptibility to lung cancer among cigarette smokers.


METHODS
DNA was obtained from blood samples and we studied by PCR-RFLP the distribution of CYP1A1 *2B (n=248) and *4 (n=222) polymorphisms in healthy controls and 222 lung cancer patients from a Mexican population.


RESULTS
Comparisons between groups showed an increased risk for lung cancer patients of *2B/*2B (18%; OR 7.6; 95% CI 3.0-19.2) and *4/ *4 genotypes (15%; OR 11.45; 95% CI 2.19-59.85) compared to the control group (1% for *2B/ *2B and 4.4% for *4/ *4). A significant association between lung cancer and homozygous *2B/ *2B passive smokers and *4/*4 ever (cigarettes) and passive smokers was also observed (p<0.05). Multivariate analysis revealed an increased risk for the *2B/*2B genotype (OR 6.83), as well as for *4/*4 (OR 28.8).


CONCLUSIONS
The results of the study indicate a significant association between *2B/*2B and *4/*4 genotypes and the risk of developing lung cancer among Mexicans.
Gallegos-Arreola
M P
MP
Molecular Genetics Laboratory, Division of Molecular Medicine, Centro de Investigación Biomédica de Occidente, Guadalajara, Jalisco - Mexico. marthaga@foreigner.class.udg.mx
Figuera-Villanueva
L E
LE
Troyo-Sanroman
R
R
Morgán-Villela
G
G
Puebla-Pérez
A M
AM
Flores-Marquez
M R
MR
Zúniga-González
G M
GM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D001483:Base Sequence; D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000209:etiology / Q000235:genetics*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D014028:Tobacco Smoke Pollution / Q000009:adverse effects
D017931:DNA Primers; D004273:DNA, Neoplasm; D014028:Tobacco Smoke Pollution; D019363:Cytochrome P-450 CYP1A1