title: Silencing of hSlo potassium channels in human osteosarcoma cells promotes tumorigenesis.
abstract: Potassium channels, the most diverse superfamily of ion channels, have recently emerged as regulators of carcinogenesis, thus introducing possible new therapeutic strategies in the fight against cancer. In particular, the large conductance Ca(2+)-activated K(+) channels, often referred to as BK channels, are at the crossroads of several tumor-associated processes such as cell proliferation, survival, secretion and migration. Despite the high BK channel expression in osteosarcoma (OS), their function has not yet been investigated in this malignant bone pathology. Here, using stable RNA interference to reduce the expression of hSlo, the human pore-forming alpha-subunit of the BK channel, in human Cal72 OS cells, we show that BK channels play a functional role in carcinogenesis. Our results reveal for the first time that BK channels exhibit antitumoral properties in OS in vivo and affect the tumor microenvironment through the modulation of both chemokine expression and leukocyte infiltration.
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Cambien
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.
authors.forename: Roger
authors.initials: R
authors.lastname: Rezzonico
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Vitale
authors.affiliation: Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS UPR 9040, Gif-sur-Yvette, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Rouzaire-Dubois
authors.affiliation: Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS UPR 9040, Gif-sur-Yvette, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Dubois
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Barthel
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.|CHU, Hôpital de l'Archet II, Service de Chirurgie Générale et Cancérologie Digestive, Nice F-06202, France.
authors.forename: Babou Karimdjee
authors.initials: BK
authors.lastname: Soilihi
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM ERI 21, Nice F-06107, France.
authors.forename: Baharia
authors.initials: B
authors.lastname: Mograbi
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Schmid-Alliana
authors.affiliation: Université de Nice Sophia Antipolis, UFR Sciences, Nice F-06002, France.|INSERM, U638, Nice F-06107, France.
authors.forename: Heidy
authors.initials: H
authors.lastname: Schmid-Antomarchi
mesh_terms: D015152:Blotting, Northern; D001859:Bone Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: C494841:KCNMA1 protein, human; D051037:Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; D034741:RNA, Small Interfering