title: The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome.
abstract: The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.
authors.affiliation: Department of Biomedical Sciences, Protein Chemistery, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
authors.forename: Kambiz
authors.initials: K
authors.lastname: Gilany
authors.forename: Roos
authors.initials: R
authors.lastname: Van Elzen
authors.forename: Kim
authors.initials: K
authors.lastname: Mous
authors.forename: Edmond
authors.initials: E
authors.lastname: Coen
authors.forename: Walter
authors.initials: W
authors.lastname: Van Dongen
authors.forename: Stefaan
authors.initials: S
authors.lastname: Vandamme
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Evy
authors.initials: E
authors.lastname: Timmerman
authors.forename: Joël
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dewilde
authors.forename: Xaveer
authors.initials: X
authors.lastname: Van Ostade
authors.forename: Luc
authors.initials: L
authors.lastname: Moens
mesh_terms: D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome