title: Use of multilocus sequence typing for the investigation of colonisation by Candida albicans in intensive care unit patients.
abstract: A prospective study was performed to determine the prevalence of candidal colonisation on the general intensive care unit at a large teaching hospital. Colonisation with Candida spp. was found to be common, occurring in 79% of patients on the unit. C. albicans was the commonest species, colonising 64% of patients, followed by C. glabrata (18%) and C. parapsilosis (14%). Most of the members of staff tested carried Candida spp. at some point, although carriage appeared to be transient. C. parapsilosis was the most commonly isolated species from staff hands, whereas C. albicans was the most commonly isolated species from the mouth. The molecular epidemiology of C. albicans was investigated using Ca3 typing and multilocus sequence typing (MLST). MLST proved to be a reproducible typing method and a useful tool for the investigation of the molecular epidemiology of C. albicans. The results of the molecular typing provided evidence for the presence of an endemic strain on the unit, which was isolated repeatedly from patients and staff. This finding suggests horizontal transmission of C. albicans on the unit though it may also reflect the relative frequency of C. albicans strain types colonising patients on admission. This study has important implications for the epidemiology of systemic candidal infections.
authors.affiliation: Mycology Reference Centre, The General Infirmary at Leeds, Leeds, UK.
authors.forename: P R
authors.initials: PR
authors.lastname: Cliff
authors.forename: J A T
authors.initials: JA
authors.lastname: Sandoe
authors.forename: J
authors.initials: J
authors.lastname: Heritage
authors.forename: R C
authors.initials: RC
authors.lastname: Barton
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002177:Candidiasis / Q000453:epidemiology / Q000382:microbiology*; D002353:Carrier State / Q000382:microbiology*; D015342:DNA Probes; D005260:Female; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D007362:Intensive Care Units; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D016533:Mycological Typing Techniques; D015995:Prevalence; D017422:Sequence Analysis, DNA
chemical_list: D015342:DNA Probes; D005656:Fungal Proteins