Electronic volume of CD34 positive cells from peripheral blood apheresis samples.
BACKGROUND
The authors have used a flow analyzer to measure electronic cellular volume of peripheral blood hematopoietic stem/progenitor cells obtained by granulocyte-colony stimulating factor (G-CSF) mobilization and apheresis (HPC-A) of patients with hematological malignancies.


METHODS
Fifty three apheresis samples stained with CD45-fluorescein isothiocyanate (FITC) and CD34-R-phycoerythrin (PE)-labeled antibodies after erythrocyte lysis with BD FACS Lysing Solution were analyzed for electronic cell volume and two-color FITC and PE fluorescence.


RESULTS
Lymphocytes, monocytes and granulocytes in the HPC-A samples had a mean electronic volume of 414, 797, and 670 microm(3), respectively corresponding to cell diameter of 9.25, 11.5, and 10.85 microm. In 53 HPC-A samples analyzed, the mean electronic volume of the CD34 positive mononuclear cells was 407 microm(3) while the CD45 positive cells had mean volume of 453 microm(3).


CONCLUSIONS
CD34 positive stem/progenitor cells have a smaller volume and diameter than CD45 positive mononuclear cells in HPC-A samples. In the present study the CD34 stem/progenitor cells had a considerably larger diameter than that of stem cells previously reported in the literature. With the availability of electronic cell volume as a parameter in flow cytometric analysis, further studies can be carried out to correlate stem cell volume with specific phenotypic marker expression.
Shariatmadar
Sherry
S
Department of Pathology, Jackson Memorial Medical Center and Sylvester Cancer Center, University of Miami Miller School of Medicine, Miami 33101, Florida, USA.
Sharma
Siddharth
S
Cabana
Raquel
R
Powell
Scott
S
Ruiz
Phillip
P
Krishan
Awtar
A
D018952:Antigens, CD34 / Q000097:blood*; D001781:Blood Component Removal; D002469:Cell Separation; D048429:Cell Size; D005434:Flow Cytometry / Q000379:methods* / Q000706:statistics & numerical data; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology*; D006801:Humans; D010799:Phycoerythrin
D018952:Antigens, CD34; D005456:Fluorescent Dyes; D010799:Phycoerythrin; D016650:Fluorescein-5-isothiocyanate