[Multiplication of Brucella abortus and production of nitric oxide in two macrophage cell lines of different origin].
Brucella abortus is a bacterium which causes abortions and infertility in cattle and undulant fever in humans. It multiplies intracellularly, evading the mechanisms of cellular death. Nitric oxide (NO) is important in the regulation of the immune response. In the present work, we studied the ability of three B. abortus strains to survive intracellularly in two macrophage cell lines. The bacterial multiplication in both cell lines was determined at two different times in UFC/ ml units. Moreover the inoculated cells were also observed under light-field and fluorescence microscopy stained with Giemsa and acridine orange, respectively. The stain of both cellular lines showed similar results with respect to the UFC/ml determination. The presence of B. abortus was confirmed by electronic microscopy. In both macrophage cell lines inoculated with the rough strain RB51, the multiplication diminished and the level of NO was higher, compared with cells inoculated with smooth strains (S19 and 2308). These results suggest that the absence of O-chain of LPS probably affects the intracellular growth of B. abortus.
Serafino
J
J
Instituto Nacional de Tecnología Agropecuaria, INTA-CICVyA-Castelar, Instituto de Patobiología, Area Bacteriología, Sección Brucelosis, Castelar, Buenos Aires, Argentina.
Conde
S
S
Zabal
O
O
Samartino
L
L
D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry / Q000502:physiology*; D002003:Brucella abortus / Q000145:classification / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D002417:Cattle; D002455:Cell Division; D002460:Cell Line / Q000378:metabolism / Q000382:microbiology; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008854:Microscopy, Electron; D009569:Nitric Oxide / Q000096:biosynthesis*; D019081:O Antigens / Q000502:physiology; D013045:Species Specificity
D019081:O Antigens; D009569:Nitric Oxide