Use of capillary electrophoresis for polysaccharide studies and applications.
Capillary electrophoresis (CE) applications to charged polysaccharides are briefly reported. A simple procedure is presented to determine the esterification degree of a hyaluronan derivative. In this case, the degree of substitution was as low as 14%. The molecular weight distribution of mannuronic oligosaccharides mixture produced by hydrolysis of native polymannuronic is readily calculated from peak area of the species resolved by CE on the basis of a specific degree of polymerization. The influence of the applied electric field strength on the free solution mobility of hyaluronan samples is briefly addressed for molar masses of the order of 10(5) and 10(6) g/mol. The data are compared with the results obtained for a 50% galactose-substituted hyaluronic acid (HA). Mobility data obtained as a function of buffer pH for a native HA sample as well as for two galactose-amide HA derivatives, having slightly different degrees of substitution, are presented and discussed in terms of the polymer charge density parameters xi. In most cases, more questions than answers arise from the application of CE to charged polysaccharides. However, perspectives are disclosed for a further understanding of the reliability of CE applied for the structural elucidation of such macromolecules.
Gamini
Amelia
A
Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, Italy.
Coslovi
Anna
A
Rustighi
Isabella
I
Campa
Cristiana
C
Vetere
Amedeo
A
Paoletti
Sergio
S
D000818:Animals; D020148:Butyric Acid / Q000032:analysis; D020374:Chromatography, Micellar Electrokinetic Capillary; D004560:Electricity; D019075:Electrophoresis, Capillary / Q000379:methods*; D004952:Esters / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D014466:Ultraviolet Rays
D004952:Esters; D009844:Oligosaccharides; D011134:Polysaccharides; D020148:Butyric Acid; D006820:Hyaluronic Acid