A highly fluorescent nucleoside analog based on thieno[3,4-d]pyrimidine senses mismatched pairing.
A highly emissive nucleobase analog, based on a thieno[3,4-d]pyrimidine core, is enzymatically incorporated into RNA oilgonucleotides that function as base discriminating fluorescent probes.
Srivatsan
Seergazhi G
SG
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0358, USA.
Weizman
Haim
H
Tor
Yitzhak
Y
D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
C529750:7-ribofuranosylthieno(3,4-d)pyrimidine-3,6-dione; D005456:Fluorescent Dyes; D009705:Nucleosides; D009841:Oligonucleotides; D012313:RNA; D004247:DNA