title: Osteoblastic cell behavior on nanostructured metal implants.
abstract: OBJECTIVE
  Surface modifications at the nanometric scale may promote protein adsorption, cell adhesion and thus favor the osseointegration of metal implants. The behavior of osteoblastic cells was studied on mirror-polished (Smooth-SS) and nanostructured (Nano-SS) stainless steel surfaces.
  
  
  METHODS
  The nanostructuration was obtained by anodization. The surfaces were characterized by scanning electron, atomic force microscopy (AFM) and X-ray photoelectron specytoscopy. Osteoblastic cells were cultured on these surfaces.
  
  
  CONCLUSIONS
  AFM showed a regular array of pores on the Nano-SS. Osteoblastic cells spread more rapidly on Nano-SS surfaces than on Smooth-SS surfaces. Cell viability was similar on Smooth-SS and Nano-SS. Alkaline phosphatase activity increased with culture time whatever the substrate but was better enhanced at 21 days on the Nano-SS than on the Smooth-SS.
  
  
  CONCLUSIONS
  This study shows that the nanostructuration of metal implants may improve the adhesion and differentiation of osteoblastic cells.
authors.affiliation: INSERM U791, Laboratory for Osteo-Articular & Dental Tissue Engineering, Faculty of dDental Surgery, 44042 Nantes, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Le Guehennec
authors.forename: Frantz
authors.initials: F
authors.lastname: Martin
authors.forename: Marco-Antonio
authors.initials: MA
authors.lastname: Lopez-Heredia
authors.forename: Guy
authors.initials: G
authors.lastname: Louarn
authors.forename: Yves
authors.initials: Y
authors.lastname: Amouriq
authors.forename: Jacques
authors.initials: J
authors.lastname: Cousty
authors.forename: Pierre
authors.initials: P
authors.lastname: Layrolle
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002454:Cell Differentiation; D002470:Cell Survival; D008422:Materials Testing; D051379:Mice; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010012:Osteogenesis / Q000502:physiology*; D013193:Stainless Steel / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D013193:Stainless Steel