title: Rapid isolation of host-independent Bdellovibrio bacteriovorus.
abstract: A new method of isolating host-independent Bdellovibrio bacteriovorus has been developed. Filtered suspensions of host-dependent cells are dropped in small volumes onto 0.2 microm membranes laid on rich media agar. Significant growth is observed within 1-2 days; these cells were confirmed to be B. bacteriovorus using microscopic observations and PCR.
authors.affiliation: Department of Chemistry, SUNY New Paltz, 1 Hawk Drive, New Paltz, NY, 12561, USA.
authors.forename: Megan A
authors.initials: MA
authors.lastname: Ferguson
authors.forename: Jaclyn L
authors.initials: JL
authors.lastname: Schmitt
authors.forename: Anil R
authors.initials: AR
authors.lastname: Sindhurakar
authors.forename: Catherine B
authors.initials: CB
authors.lastname: Volle
authors.forename: Megan E
authors.initials: ME
authors.lastname: Nuñez
authors.forename: Eileen M
authors.initials: EM
authors.lastname: Spain
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001501:Bdellovibrio / Q000166:cytology / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D008853:Microscopy; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial