Solid-phase synthesis of pseudo-complementary peptide nucleic acids.
Pseudo-complementary peptide nucleic acid (pcPNA) is a DNA analog in which modified DNA bases 2,6-diaminopurine (D) and 2-thiouracil (U(s)) 'decorate' a poly[N-(2-aminoethyl)glycine] backbone, together with guanine (G) and cytosine (C). One of the most significant characteristics of pcPNA is its ability to effect double-duplex invasion of predetermined DNA sites inducing various changes in the biological and the physicochemical properties of the DNA. This protocol describes solid-phase synthesis of pcPNA. The monomers for G and C are commercially available, but the monomers for D and U(s) need to be synthesized (or can be ordered to custom synthesis companies). Otherwise, the procedure is the same as that employed for Boc-strategy synthesis of conventional PNA. This protocol, if the synthesis of D and U(s) monomers is not factored in, takes approximately 7 d to complete.
Komiyama
Makoto
M
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan. komiyama@mkomi.rcast.u-tokyo.ac.jp
Aiba
Yuichiro
Y
Ishizuka
Takumi
T
Sumaoka
Jun
J
D015075:2-Aminopurine / Q000031:analogs & derivatives / Q000737:chemistry; D015394:Molecular Structure; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis*; D013889:Thiouracil / Q000737:chemistry
D020135:Peptide Nucleic Acids; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D013889:Thiouracil