title: Synthesis of a series of fructooligosaccharides with sucrose and cycloinulohexaose extending over ten degrees of polymerization using cycloinulooligosaccharide fructanotransferase from Bacillus circulans OKUMZ 31B.
abstract: Prolonged incubation with sucrose as an acceptor and cycloinulohexaose as a donor at a high concentration of cycloinulooligosaccharide fructanotransferase afforded a series of fructooligosaccharides. Their chain-length distribution extended over 10 degrees of polymerization when the donor concentration was increased to 120 mM. Increasing the acceptor concentration proved effective in improving the yield of inulin-type oligosaccharides because hydrolysis was suppressed.
authors.affiliation: Department of Biology, Osaka Kyoiku University, Kashiwara, Osaka 582-8582, Japan. kawamura@cc.osaka-kyoiku.ac.jp
authors.forename: Mishio
authors.initials: M
authors.lastname: Kawamura
authors.forename: Natsue
authors.initials: N
authors.lastname: Matsuda
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005632:Fructose / Q000031:analogs & derivatives* / Q000378:metabolism; D006602:Hexosyltransferases / Q000737:chemistry / Q000378:metabolism*; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism*; D013395:Sucrose / Q000737:chemistry*; D013997:Time Factors
chemical_list: D001704:Biopolymers; D009844:Oligosaccharides; D005632:Fructose; D013395:Sucrose; D006602:Hexosyltransferases; C062589:cycloinulo-oligosaccharide fructanotransferase