Gene profiling in human blood leucocytes during recovery from septic shock.
OBJECTIVE
To assess blood leucocytes gene profiling during recovery phase of septic shock; to test the relation between encoding gene expression and protein level.


METHODS
Gene expression levels were studied at days 0, 1, 7 and 28 (D0, 1, 7 and 28) on a dedicated microarray of 340 genes involved in inflammatory processes.


METHODS
16-bed intensive care unit, Lariboisière University hospital.


METHODS
Seventeen septic shock patients enrolled when at least one additional organ dysfunction occurred.


RESULTS
Changes over time were compared with D0 via the ratio Dx/D0. The time-related gene expression study showed significant changes in ten genes. Among them, S100A8 and S100A12 had a reduced expression over time compared with D0, whereas CD74's expression increased. The microarray results were validated by RT-qPCR for four genes. The S100A8 plasma levels decrease along recovery in parallel with the gene expression decrease. The CD74 gene expression evolution significantly correlated with HLA-DR monocyte expression.


CONCLUSIONS
These results are the first description of variations in expression of key inflammatory genes in the course of the septic shock recovery period.
Payen
Didier
D
Lariboisière University Hospital, Assistance Publique - Hôpitaux de Paris, Department of Anesthesiology and Critical Care Medicine, Paris, France. dpayen1234@aol.com
Lukaszewicz
Anne-Claire
AC
Belikova
Ioulia
I
Faivre
Valérie
V
Gelin
Catherine
C
Russwurm
Stefan
S
Launay
Jean-Marie
JM
Sevenet
Nicolas
N
D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics; D040501:Calgranulin A / Q000097:blood* / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006801:Humans; D007962:Leukocytes; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012772:Shock, Septic / Q000235:genetics* / Q000401:mortality; D016019:Survival Analysis
D000944:Antigens, Differentiation, B-Lymphocyte; D040501:Calgranulin A; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C466441:HLA-DRB1*12:01:01 antigen; D000949:Histocompatibility Antigens Class II; C045084:invariant chain