Identification of glutathione sulfotransferase-pi (GSTP1) as a new resveratrol targeting protein (RTP) and studies of resveratrol-responsive protein changes by resveratrol affinity chromatography.
BACKGROUND
Resveratrol shows chemopreventive and other biological affects in in vitro and some animal studies. The bioactivities of resveratrol may be attributed to qualitative and quantitative differences in its cell-type-specific interaction and binding with its cellular targets, denoted as resveratrol targeting proteins (RTPs).


METHODS
To isolate RTPs, resveratrol was linked to epoxy-activated agarose generating an affinity platform to allow the isolation, purification, and characterization of distinct RTPs from cultured prostate cancer cell extracts.


RESULTS
Glutathione sulfotransferase-pi (GSTP1) and estrogen receptor-beta (ER-beta) were found to be new RTPs. Resveratrol affinity chromatography was shown to be an easy method for analyzing resveratrol-responsive protein changes in the androgen-dependent LNCaP cells.


CONCLUSIONS
Resveratrol affects cellular functions at multiple levels, ranging from interaction with detoxification enzymes, such as GSTP1 and transcription by targeting factors such as ER-beta.
Hsieh
Tze-Chen
TC
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA.
Wang
Zhirong
Z
Deng
Haiteng
H
Wu
Joseph M
JM
D000595:Amino Acid Sequence; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity / Q000379:methods; D051549:Glutathione S-Transferase pi / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D011485:Protein Binding; D011808:Quinone Reductases / Q000737:chemistry / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D008024:Ligands; D013267:Stilbenes; D011808:Quinone Reductases; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D000077185:Resveratrol