Identification of thermotolerant campylobacter species by fluorescence in situ hybridization.
Thermotolerant Campylobacter spp. (Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization (FISH) for the identification of Campylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates of Campylobacter, Arcobacter, and Helicobacter. FISH correctly identified all isolates to the genus level and detected all thermotolerant Campylobacter isolates. The assay showed high degrees of sensitivity for the identification of C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the species level.
Poppert
Sven
S
Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany. poppert@bni-hamburg.de
Haas
Michaela
M
Yildiz
Tatjana
T
Alter
Thomas
T
Bartel
Edda
E
Fricke
Ursula
U
Essig
Andreas
A
D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003470:Culture Media; D015342:DNA Probes; D006358:Hot Temperature; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D013045:Species Specificity
D003470:Culture Media; D015342:DNA Probes