title: Acrosomal and viability status of bovine spermatozoa evaluated by two staining methods.
abstract: Artificial insemination with frozen-thawed spermatozoa is commonly used in cattle breeding. A simple and fast procedure is needed for routine evaluation of the acrosomal status of frozen-thawed bovine sperm. Therefore, the purpose of this study was to test two staining procedures used to determine the viability and integrity of acrosome of frozen-thawed bovine spermatozoa. Double staining and Hoechst/FITC-Pisum sativum agglutinin (FITC-PSA) labelling were tested for evaluating the viability and acrosome reaction induced by calcium ionophore of bull spermatozoa. In our experiments no significant differences were detected in the frequency of acrosome-reacted sperm either by double staining (37.98%) or by FITC-PSA labelling (39.33%). The viability of sperm stained by the double staining method was 67.17%, and a higher portion of viable sperm (82.67%) was observed by staining with the Hoechst procedure (P < 0.01). On the basis of the results obtained it is concluded that both methods can be used for detecting the acrosome reaction of frozen-thawed bovine spermatozoa.
authors.affiliation: Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Moyzesova 61, 900 28 Ivanka pri Dunaji, Slovak Republic. jana.jankovicova@savba.sk
authors.forename: Jana
authors.initials: J
authors.lastname: Jankovicová
authors.forename: Michal
authors.initials: M
authors.lastname: Simon
authors.forename: Jana
authors.initials: J
authors.lastname: Antalíková
authors.forename: L'ubica
authors.initials: L
authors.lastname: Horovská
mesh_terms: D000177:Acrosome / Q000502:physiology*; D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D002417:Cattle / Q000502:physiology*; D002470:Cell Survival; D005615:Freezing; D008297:Male; D012662:Semen Preservation / Q000662:veterinary; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary*