Loss of methylation imprint of Snrpn in postovulatory aging mouse oocyte.
Prolonged residence of postovulatory oocyte in the oviduct or prolonged culture in vitro can lead to oocyte aging, which significantly affects pre- and post-implantation embryo development. In this study, we employed bisulfite sequencing and COBRA methods to investigate the DNA methylation status of differentially methylated regions (DMRs) of Snrpn and Peg1/Mest, two maternally imprinted genes, in postovulatory oocytes aged in vivo and in vitro. The results showed that Snrpn DMR was clearly demethylated in oocytes aged in vivo at 29h post-hCG and in denuded oocytes aged in vitro for the same time period. However, Peg1/Mest did not show any demethylation in all aged groups at 29h post-hCG. These data indicate that oocytes undergo time-dependent demethylation of Snrpn DMR during the process of postovulatory aging.
Liang
Xing-Wei
XW
Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530004, China.
Zhu
Jia-Qiao
JQ
Miao
Yi-Liang
YL
Liu
Jing-He
JH
Wei
Liang
L
Lu
Sheng-Sheng
SS
Hou
Yi
Y
Schatten
Heide
H
Lu
Ke-Huan
KH
Sun
Qing-Yuan
QY
D000818:Animals; D001324:Autoantigens / Q000235:genetics*; D016922:Cellular Senescence / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D019175:DNA Methylation; D005260:Female; D018392:Genomic Imprinting; D051379:Mice; D009865:Oocytes / Q000378:metabolism / Q000502:physiology*; D011506:Proteins / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013447:Sulfites / Q000737:chemistry; D055517:snRNP Core Proteins
D001324:Autoantigens; D011506:Proteins; D017411:Ribonucleoproteins, Small Nuclear; D013447:Sulfites; C103637:mesoderm specific transcript protein; D055517:snRNP Core Proteins; D004247:DNA; C042345:hydrogen sulfite