Biochemical changes in rat testis induced in vitro by reactive oxygen species.
We report the effects of reactive oxygen species generated by ultraviolet-A radiation on some biochemical parameters specific for oxidative stress, in rat testis homogenates. Results show an increase in lipid peroxidation products under ultraviolet-A exposure, and suggest that the involved mechanism is typical for a radical-mediated chain reaction. The amount of SH groups also increases during irradiation, probably as a consequence of conformational changes in proteins. Electrophoresis results revealed protein pattern changes mainly in the low molecular weight domain. The catalytic activities of alkaline phosphatase and gamma-glutamil transpeptidase are modified under the oxidative conditions generated by reactive oxygen species. The changes of the enzymatic activities are UVA exposure time-dependent, suggesting that conformational modifications are responsible for enzymatic activities enhancement.
Nechifor
Marina Tamara
MT
University of Bucharest, Faculty of Biology, 91-95 Splaiul Independentei, Bucharest, Romania. nemar@bio.bio.unibuc.ro
Constantin
Carolina
C
Manda
Gina
G
Neagu
Monica
M
Dinu
Diana
D
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000097:blood; D013737:Testis / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase