Analysis of gene expression in normal and cancer cells exposed to gamma-radiation.
The expression of many genes is modulated after exposure to ionizing radiation. Identification of specific genes may allow the determination of pathways important in radiation responses. We previously identified modulation of the expression of several genes in response to ionizing radiation treatment. In the present study, we monitored the expression of RGS1, CC3, THBS1, vWF, MADH7, and a novel gene encoding a secreted protein in irradiated Jurkat, TK6, HeLa, and HFL1 cells. The RGS1 is involved in G-protein signaling pathway, CC3 belongs to the complement system, THBS1 is a component of the extracellular matrix, vWF takes part in blood coagulation, and MADH7 is a member of the TGF-beta signal transduction pathway. Our objective was to find similarities and differences in the expression of these genes in ionizing radiation-exposed diverse cell types. RGS1 was downregulated in Jurkat cells but was upregulated in TK6 and HFL1 cells. The expression of CC3 was repressed in Jurkat and HFL1 cells but was induced in TK6 and HeLa cells. THBS1 was downregulated in irradiated TK6 and HFL1 cells. vWF was induced in radiation-exposed HeLa cells, but its expression was downregulated in Jurkat cells. The expression of MADH7 was induced in all the cell types examined. These results indicate cell specific modulation of gene expression and suggest the involvement of different pathways in cellular response to radiation treatment in different cells.
Chaudhry
M Ahmad
MA
Department of Medical Laboratory and Radiation Sciences, University of Vermont, 302 Rowell Building, Burlington, 05405 VT, USA. mchaudhr@uvm.edu
D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D015870:Gene Expression / Q000528:radiation effects*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D019169:Jurkat Cells; D009363:Neoplasm Proteins / Q000378:metabolism*; D011829:Radiation Dosage; D015398:Signal Transduction / Q000528:radiation effects*
D009363:Neoplasm Proteins