[Neoretinal antigen expression: a comparison of anatomical and clinical features of a murine uveoretinitis model].
OBJECTIVE
Uveitis is an inflammation involving the retina. The antigens targeted by the experimental models are located in the pigmentary epithelium-photoreceptor complex. To gain insights into the variations in topographic expression of the antigen in the retina, we studied a new mouse model.


METHODS
and methods: Stable retinal expression of the influenza virus hemagglutinin (HA) was obtained after intravitreal or subretinal injection of recombinant adeno-associated virus carrying HA (AAV-HA). One month later, we transferred HA-specific T cells, followed by a subcutaneous immunization of the cognate antigen emulsified in CFA. The animals were clinically examined with a slit lamp biomicroscope. Infiltration of donor cells was detected by immunostaining on retina flatmounts with anti-Thy-1.1 antibody, and infiltrating cells were studied using FACS analysis.


RESULTS
Whatever the location of the HA expression, intraocular inflammation was clinically and histologically detected in all animals, between 10 and 15 days after immunization with HA. Lesions were identified with histopathological analysis. The ocular infiltrate was mostly composed of macrophages and HA-specific T cells in different proportions.


CONCLUSIONS
The topographic variations of targeted ocular antigens do not seem to modify the development of inflammatory reactions in our model. By targeting different antigen-presenting cells, ocular infiltrating cells are different.
Terrada
C
C
CNRS UMR 7087, CERVI, Hôpital de la Pitié-Salpétrière, Paris. cterrada@chups.jussieu.fr
Pâques
M
M
Fisson
S
S
De Kozak
Y
Y
Klatzmann
D
D
Salomon
B
B
LeHoang
P
P
Bodaghi
B
B
D000818:Animals; D000941:Antigens / Q000032:analysis; D000229:Dependovirus / Q000276:immunology; D004195:Disease Models, Animal; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis*; D051379:Mice; D012160:Retina / Q000276:immunology* / Q000821:virology; D012173:Retinitis / Q000469:parasitology* / Q000503:physiopathology*; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D014605:Uveitis / Q000473:pathology* / Q000503:physiopathology*; D014822:Vitreous Body / Q000276:immunology
D000941:Antigens; D019267:Hemagglutinin Glycoproteins, Influenza Virus