title: Surface mobility of postsynaptic AMPARs tunes synaptic transmission.
abstract: AMPA glutamate receptors (AMPARs) mediate fast excitatory synaptic transmission. Upon fast consecutive synaptic stimulation, transmission can be depressed. Recuperation from fast synaptic depression has been attributed solely to recovery of transmitter release and/or AMPAR desensitization. We show that AMPAR lateral diffusion, observed in both intact hippocampi and cultured neurons, allows fast exchange of desensitized receptors with naïve functional ones within or near the postsynaptic density. Recovery from depression in the tens of millisecond time range can be explained in part by this fast receptor exchange. Preventing AMPAR surface movements through cross-linking, endogenous clustering, or calcium rise all slow recovery from depression. Physiological regulation of postsynaptic receptor mobility affects the fidelity of synaptic transmission by shaping the frequency dependence of synaptic responses.
authors.affiliation: CNRS, UMR 5091, Université Bordeaux, Bordeaux, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Heine
authors.forename: Laurent
authors.initials: L
authors.lastname: Groc
authors.forename: Renato
authors.initials: R
authors.lastname: Frischknecht
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Béïque
authors.forename: Brahim
authors.initials: B
authors.lastname: Lounis
authors.forename: Gavin
authors.initials: G
authors.lastname: Rumbaugh
authors.forename: Richard L
authors.initials: RL
authors.lastname: Huganir
authors.forename: Laurent
authors.initials: L
authors.lastname: Cognet
authors.forename: Daniel
authors.initials: D
authors.lastname: Choquet
mesh_terms: D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004058:Diffusion; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials; D036681:Fluorescence Recovery After Photobleaching; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007736:Kynurenic Acid / Q000494:pharmacology; D009473:Neuronal Plasticity; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects; D014162:Transfection
chemical_list: D002118:Calcium; D018691:Excitatory Amino Acid Antagonists; D018698:Glutamic Acid; D007736:Kynurenic Acid; D018091:Receptors, AMPA; D011993:Recombinant Fusion Proteins; C104722:glutamate receptor ionotropic, AMPA 2