A paper-based electroelution system for protein recovery from stained sodium dodecyl sulfate-polyacrylamide gels.
Electroelution is a widely used methodology for protein purification. In this study, a practical and low-cost system for protein electroelution from stained polyacrylamide gels was developed. For this, a horizontal protein electroelution cuve was constructed with glass plates, 1.5-ml capacity microcentrifuge tubes, and dialysis membrane. Analyses of the system efficiency showed high protein recovery from nonfixed and fixed sodium dodecyl sulfate (SDS)-polyacrylamide gels.
Branco
Alan T
AT
Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Parque Califórnia, 28015-603 Campos dos Goytacazes, RJ, Brazil.
Ferreira
Beatriz dos S
Bdos S
de Souza Filho
Gonçalo A
GA
D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013194:Staining and Labeling
D011506:Proteins; D012967:Sodium Dodecyl Sulfate