Preparation of molecularly imprinted solid phase extraction using bensulfuron-methyl imprinted polymer and clean-up for the sulfonylurea-herbicides in soybean.
A pre-treatment methodology based on the molecularly imprinted solid phase extraction (MI-SPE) procedure was developed for the determination of bensulfuron-methyl (BSM), tribenuron-methyl (TBM), metsulfuron-methyl (MSM) and nicosulfuron (NS) in soybean samples. A molecular imprinted polymer (MIP) was prepared by precipitation polymerization using BSM as the template molecule, alpha-methacrylic acid (MAA) as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the cross-linker and dichloromethane as the porogen. The binding behaviors of the template BSM and its analogues on the MIP were evaluated by high performance liquid chromatography (HPLC). Then, solid phase extraction (SPE) with a BSM molecularly imprinted polymer (BSM-MIP) as adsorbent was investigated and the optimum loading, washing, and eluting conditions for MI-SPE of the selected BSM, MSM, TBM, and NS were established. The optimized MI-SPE procedure was used to extract the sulfonylureas and a high recovery was obtained in the soybean samples.
Tang
Kaijie
K
State Key Laboratory of Food Science & Technology, Jiangnan University, Jiangsu, Wuxi 214122, China. tkjie999@sina.com
Chen
Shangwei
S
Gu
Xiaohong
X
Wang
Haijun
H
Dai
Jun
J
Tang
Jian
J
D002138:Calibration; D004566:Electrodes; D006540:Herbicides / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*; D013453:Sulfonylurea Compounds / Q000032:analysis* / Q000737:chemistry
D006540:Herbicides; D011108:Polymers; D013453:Sulfonylurea Compounds; C071475:methyl bensulfuron