Effects of Aroclor 1254 on oxidative stress in developing Xenopus laevis tadpoles.
Over the last decades, amphibians decline has been reported worldwide. Exposure to polychlorinated biphenyls (PCBs) is one of the possible causes in addition to climate changes, UV-radiation or habitat destruction. In the present study, we tested the hypothesis that PCBs could induce oxidative stress in young tadpoles. Developing Xenopus laevis were exposed from 2- to 5-d postfertilization (pf) to 0.1 or 1 mg/l of Aroclor 1254. Lipid peroxidation and antioxidant systems (SOD, CAT, GST, GPx, GR activities and t-GSH level) were investigated in whole organisms. Exposure to both concentrations did not impact on the survival and development whereas the average body weight decreased. Exposure to 1 mg/l of Aroclor 1254 induced a significant (p<0.05) increase of GST activity when compared to controls 0 and DMSO. The other antioxidant enzymes and LPO evaluation remained unchanged. Our results demonstrate that exposure of X. laevis tadpoles to environmental concentrations of Aroclor 1254 interfere with normal growth. They also highlight that very young X. laevis tadpoles express antioxidant systems.
Gillardin
Virginie
V
Unité de Recherche en Biologie des Organismes (URBO), Facultés Universitaires Notre-Dame de la Paix, Rue de Bruxelles 61, B-5000 Namur, Belgium. virginie.gillardin@fundp.ac.be
Silvestre
Frédéric
F
Divoy
Céline
C
Thomé
Jean-Pierre
JP
Kestemont
Patrick
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D013956:Antithyroid Agents / Q000378:metabolism / Q000633:toxicity*; D001835:Body Weight; D020111:Chlorodiphenyl (54% Chlorine) / Q000378:metabolism / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism; D007814:Larva / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014982:Xenopus laevis / Q000254:growth & development / Q000378:metabolism
D000975:Antioxidants; D013956:Antithyroid Agents; D020111:Chlorodiphenyl (54% Chlorine); D013482:Superoxide Dismutase; D005982:Glutathione Transferase