The concentration of deoxyribonucleic acid in plasma from 73 patients with colorectal cancer and apparent clinical correlations.
BACKGROUND
Detection of cell-free plasma DNA has considerable potential as a tool for the diagnosis and assessment of the prognosis of many types of cancer. The aim of the present study was to quantify, by spectrophotometry, the cell-free DNA in plasma samples from patients with colorectal cancer at different stages of the disease and to attempt to correlate the resultant values with the clinical picture.


METHODS
We reviewed the medical reports of 73 patients, who had undergone resection of primary colorectal cancer. Samples of blood had been taken from each patient immediately prior to surgery. DNA was extracted from samples of plasma and quantified, by spectrophotometry, after a storage period of no longer than 2 years in 89% of the cases examined.


RESULTS
The mean(+/-S.D.) concentration of DNA in plasma samples was 108+/-156 ng/microl. We found a statistically significant correlation between the concentration of DNA and the presence of metastases (mainly liver metastases).


CONCLUSIONS
The detection and quantitation of cell-free DNA in plasma, using this simple technique, might be of clinical value for the surveillance of colon cancer patients and the detection of metastases.
Guadalajara
Héctor
H
Department of Surgery, "La Paz" University Hospital, Madrid, Spain. h_guadalajara@yahoo.com
Domínguez-Berzosa
Carolina
C
García-Arranz
Mariano
M
Herreros
María Dolores
MD
Pascual
Isabel
I
Sanz-Baro
Raquel
R
García-Olmo
Dolores C
DC
García-Olmo
Damián
D
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000302:isolation & purification; D015179:Colorectal Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D013053:Spectrophotometry
D014408:Biomarkers, Tumor; D004247:DNA