Phytate inhibits bovine pericardium calcification in vitro.
OBJECTIVE
The present study examined the inhibitory effects of pyrophosphate, etidronate, and phytate on bovine pericardium calcification in vitro.


METHODS
Bovine pericardium was glutaraldehyde fixed and then placed in a flow chamber in the presence of a synthetic physiological fluid alone (control) or the fluid plus various concentrations of pyrophosphate, etidronate, or phytate. Following a 96-h incubation, fragments were removed and assayed for calcification by measuring calcium and phosphorus levels.


RESULTS
The data indicated that both pyrophosphate and etidronate at 1 mg/l (5.75 and 4.95 microM, respectively) inhibited bovine pericardium calcification, whereas neither agent had an effect at 0.5 mg/l (2.87 and 2.47 microM, respectively). Phytate was the most potent inhibitor of calcification, and the effects of this agent were apparent at levels as low as 0.25 mg/l (0.39 microM).


CONCLUSIONS
While pyrophosphate, etidronate, and phytate were all able to inhibit bovine pericardium calcification in vitro, phytate was found to be the most effective.
Grases
Félix
F
Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS), University of Balearic Islands, Palma of Mallorca, Spain. fgrases@uib.es
Sanchis
Pilar
P
Costa-Bauzá
Antonia
A
Bonnin
Oriol
O
Isern
Bernat
B
Perelló
Joan
J
Prieto
Rafael M
RM
D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002114:Calcinosis / Q000517:prevention & control*; D002118:Calcium / Q000032:analysis; D002417:Cattle; D011756:Diphosphates / Q000494:pharmacology; D012968:Etidronic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D010496:Pericardium / Q000737:chemistry / Q000187:drug effects*; D010758:Phosphorus / Q000032:analysis; D010833:Phytic Acid / Q000494:pharmacology*
D050071:Bone Density Conservation Agents; D011756:Diphosphates; D010758:Phosphorus; D010833:Phytic Acid; D012968:Etidronic Acid; D002118:Calcium