title: High-yield production of secreted active proteins by the Pseudomonas aeruginosa type III secretion system.
abstract: The Escherichia coli system is the system of choice for recombinant protein production because it is possible to obtain a high protein yield in inexpensive media. The accumulation of protein in an insoluble form in inclusion bodies remains a major disadvantage. Use of the Pseudomonas aeruginosa type III secretion system can avoid this problem, allowing the production of soluble secreted proteins.
authors.affiliation: Laboratoire TIMC-IMAG GREPI-TheREx, Université Joseph Fourier-Grenoble 1 UMR CNRS 5525, IFR INSERM 130, Grenoble, France.
authors.forename: M
authors.initials: M
authors.lastname: Derouazi
authors.forename: B
authors.initials: B
authors.lastname: Toussaint
authors.forename: L
authors.initials: L
authors.lastname: Quénée
authors.forename: O
authors.initials: O
authors.lastname: Epaulard
authors.forename: M
authors.initials: M
authors.lastname: Guillaume
authors.forename: R
authors.initials: R
authors.lastname: Marlu
authors.forename: B
authors.initials: B
authors.lastname: Polack
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins