title: Photolabile glutamate protecting group with high one- and two-photon uncaging efficiencies.
abstract: A pi-extended [2-(2-nitrophenyl)propoxy]carbonyl (NPPOC) derivative has been prepared as an efficient UV and near-IR photolabile protecting group for glutamate. This glutamate cage compound exhibits efficient photorelease upon one-photon excitation (epsilonPhi=990 M(-1) cm(-1) at 315 nm). In addition, it also shows efficient photorelease in activation of glutamate receptors in electrophysiological recordings. Combined with a high two-photon uncaging cross-section (deltaPhi=0.45 GM at 800 nm), its overall properties make this new cage-3-(2-propyl)-4'-methoxy-4-nitrobiphenyl (PMNB)-for glutamate a very promising tool for two-photon neuronal studies.
authors.affiliation: Institut Gilbert Laustriat, Pharmacologie et Physicochimie, Faculté de Pharmacie, 74 route du Rhin, 67401 Illkirch, France.
authors.forename: Sylvestre
authors.initials: S
authors.lastname: Gug
authors.forename: Sébastien
authors.initials: S
authors.lastname: Charon
authors.forename: Alexandre
authors.initials: A
authors.lastname: Specht
authors.forename: Karine
authors.initials: K
authors.lastname: Alarcon
authors.forename: David
authors.initials: D
authors.lastname: Ogden
authors.forename: Burkhard
authors.initials: B
authors.lastname: Zietz
authors.forename: Jérémie
authors.initials: J
authors.lastname: Léonard
authors.forename: Stefan
authors.initials: S
authors.lastname: Haacke
authors.forename: Frédéric
authors.initials: F
authors.lastname: Bolze
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Nicoud
authors.forename: Maurice
authors.initials: M
authors.lastname: Goeldner
mesh_terms: D004594:Electrophysiology; D018698:Glutamic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D006624:Hippocampus / Q000378:metabolism; D015394:Molecular Structure; D018408:Patch-Clamp Techniques; D010777:Photochemistry; D017785:Photons; D013053:Spectrophotometry; D046509:Tissue Culture Techniques
chemical_list: D018698:Glutamic Acid