title: Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle.
abstract: Hepatitis E virus (HEV) accounts for the majority of enterically transmitted hepatitis infections worldwide. Currently, there is no specific treatment for or vaccine against HEV. The major structural protein is derived from open reading frame (ORF) 2 of the viral genome. A potential oral vaccine is provided by the virus-like particles formed by a protein construct of partial ORF3 protein (residue 70-123) fused to the N-terminus of the ORF2 protein (residues 112-608). Single crystals obtained by the hanging-drop vapour-diffusion method at 293 K diffract X-rays to 8.3 A resolution. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 337, b = 343, c = 346 A, alpha = beta = gamma = 90 degrees , and contain one particle per asymmetric unit.
authors.affiliation: Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
authors.forename: Che-Yen
authors.initials: CY
authors.lastname: Wang
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Miyazaki
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Yamashita
authors.forename: Akifumi
authors.initials: A
authors.lastname: Higashiura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakagawa
authors.forename: Tian-Cheng
authors.initials: TC
authors.lastname: Li
authors.forename: Naokazu
authors.initials: N
authors.lastname: Takeda
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.forename: Erik
authors.initials: E
authors.lastname: Hjalmarsson
authors.forename: Claes
authors.initials: C
authors.lastname: Friberg
authors.forename: Der-Ming
authors.initials: DM
authors.lastname: Liou
authors.forename: Yen-Jen
authors.initials: YJ
authors.lastname: Sung
authors.forename: Tomitake
authors.initials: T
authors.lastname: Tsukihara
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Miyamura
authors.forename: R Holland
authors.initials: RH
authors.lastname: Cheng
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D003460:Crystallization; D016752:Hepatitis E virus / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics; D014771:Virion / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D036022:Capsid Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins