[Expression of long isoform leptin receptor and shortest membrane bound variant in peripheral blood mononuclear cells from the obese and normal individuals].
OBJECTIVE
To investigate the expression of the long isoform leptin receptor (OB-R(L)) and the shortest membrane bound variant (OB-R(S)) in peripheral blood mononuclear cells from the obese and normal individuals (6.08 ng/ml or 31.21 ng/ml).


METHODS
Mononuclear cells were obtained from 30 obese individuals (BMI > 25 kg/m2) and 20 normal individuals (BMI < 23 kg/m2). RT-PCR was used to detect the expression of OB-R(S) and OB-R(L). The level of serum leptin was measured with immunoradiometric assay.


RESULTS
OB-R(S) was expressed in all individuals, and OB-R(L) was expressed in 38 individuals. OB-R(L) was not expressed in the 12 obese individuals with the BMI > 39 kg/m2. The expression level of OB-R(S) was 4 times higher than that of OB-R(L). There was no significant difference in the expression of either isoforms between men and women. The relative expression of both OB-R(S) and OB-R(L) isoforms was significantly lower and the serum leptin level was significantly higher in the obese individuals (6.08 ng/ml or 31.21 ng/ml). The leptin receptor expression levels were significantly negatively correlated with BMI and serum leptin level.


CONCLUSIONS
Both OB-R(S) and OB-R(L) are expressed in the peripheral blood mononuclear cells from normal and the medium obese subjects with a consistent predominance of OB-R(S). There is no significant difference in the expression of OB-R(S) and OB-R(L) between men and women. Compared with normal individuals, the expression of OB-R(S) and OB-R(L) in human mononuclear cells is lower.
Li
Qiang
Q
Department of Endocrinology, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China. hrblq@yahoo.com.cn
Li
Jing-Yi
JY
Lü
Bing-Jie
BJ
Li
Yu
Y
Zhang
Jin-Chao
JC
D000328:Adult; D015992:Body Mass Index; D005260:Female; D006801:Humans; D015592:Immunoradiometric Assay; D020738:Leptin / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000097:blood*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054411:Receptors, Leptin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020738:Leptin; D020033:Protein Isoforms; D012333:RNA, Messenger; D054411:Receptors, Leptin