A simple and inexpensive on-column frit fabrication method for fused-silica capillaries for increased capacity and versatility in LC-MS/MS applications.
A modified sol-gel method for a one-step on-column frit preparation for fused-silica capillaries and its utility for peptide separation in LC-MS/MS is described. This method is inexpensive, reproducible, and does not require specialized equipments. Because the frit fabrication process does not damage polyimide coating, the frit-fabricated column can be tightly connected on-line for high pressure LC. These columns can replace any capillary liquid transfer tubing without any specialized connections up-stream of a spray tip column. Therefore multiple columns with different phases can be connected in series for one- or multiple-dimensional chromatography.
Wang
Ling-Chi
LC
Department of Molecular Microbiology and Immunology, USC Norris Cell and Tissue Imaging Core, USC/Norris Comprehensive Cancer Center, Keck School of Medicine at USC, Los Angeles, CA, USA.
Okitsu
Cindy Yen
CY
Kochounian
Harold
H
Rodriguez
Anthony
A
Hsieh
Chih-Lin
CL
Zandi
Ebrahim
E
D000818:Animals; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D044367:Phase Transition; D040901:Proteomics / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013696:Temperature
D012822:Silicon Dioxide