title: Toxicokinetic approach for the assessment of endocrine disruption effects of contaminated dredged material using female Carcinus maenas.
abstract: Ecotoxicological effects associated with contaminants present in dredged material from three Spanish ports, Ría of Huelva (SW, Spain), La Coruña (NW, Spain) and Bay of Cádiz (SW, Spain) were determined using a marine biotest based on endocrine disruption effects (vitellogenesis process alteration). Intermoult female Carcinus maenas were exposed in the laboratory to sediments from the Spanish ports per replicate during 21 days. Crab haemolymph samples were taken for vitellogenin/vitellin (VTG) analysis on days 0, 7, 14 and 21. Furthermore, chemical analysis was performed in the stations to determine the degree and nature of sediment contamination (Cr, Ni, Cu, Zn, Cd, Pb, Hg, As, PAHs and PCBs). A significant induction (P < 0.05) in vitellogenin/vitellin concentration was observed over time in individuals exposed to sediment samples containing significant concentrations of PAHs, PCBs, Hg, Pb and Zn. The toxicokinetic approach, proposed in this study related to the use of this biomarker in C. maenas, proved to be a powerful and sensitive tool to evaluate toxicity effects associated with contaminants present in dredged material. Moreover the integration of the results obtained through multivariate analysis approach (MAA) allowed the identification contaminants bound to sediments associated with adverse effects, validating the use of this marine biotest in a regulatory framework.
authors.affiliation: Instituto de Ciencias, Marinas de Andalucía, Campus Río Pedro s/n, Puerto Real, Cadiz, Spain. laura.martin@uca.es
authors.forename: M Laura
authors.initials: ML
authors.lastname: Martín-Díaz
authors.forename: Diego
authors.initials: D
authors.lastname: Sales
authors.forename: Angel
authors.initials: A
authors.lastname: DelValls
mesh_terms: D000818:Animals; D001682:Biological Availability; D015415:Biomarkers / Q000378:metabolism; D003386:Brachyura / Q000187:drug effects* / Q000378:metabolism; D052244:Endocrine Disruptors / Q000493:pharmacokinetics / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D019015:Geologic Sediments / Q000737:chemistry; D006458:Hemolymph / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D019216:Metals, Heavy / Q000493:pharmacokinetics / Q000633:toxicity; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000493:pharmacokinetics / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics / Q000633:toxicity; D013030:Spain; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls