Fluorescence intensity and intermittency as tools for following dopamine bioconjugate processing in living cells.
CdSe/ZnS quantum dots (QDs) conjugated to biomolecules that quench their fluorescence, particularly dopamine, have particular spectral properties that allow determination of the number of conjugates per particle, namely, photoenhancement and photobleaching. In this work, we quantify these properties on a single-particle and ensemble basis in order to evaluate their usefulness as a tool for indicating QD uptake, breakdown, and processing in living cells. This creates a general framework for the use of fluorescence quenching and intermittency to better understand nanoparticle-cell interactions.
Khatchadourian
Rafael
R
Department of Biomedical Engineering, McGill University, 3775 Rue University, 316 Lyman Duff Medical Building, Montréal, Canada.
Bachir
Alexia
A
Clarke
Samuel J
SJ
Heyes
Colin D
CD
Wiseman
Paul W
PW
Nadeau
Jay L
JL
D000465:Algorithms; D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D006801:Humans; D009474:Neurons / Q000378:metabolism*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*
D004298:Dopamine