Endosymbionts of Siboglinum fiordicum and the phylogeny of bacterial endosymbionts in Siboglinidae (Annelida).
Siboglinid worms are a group of gutless marine annelids that are nutritionally dependent upon endosymbiotic bacteria. Four major groups of siboglinids are known-vestimentiferans, moniliferans, Osedax spp. and frenulates. Although endosymbionts of vestimentiferans and Osedax spp. have been previously characterized, little is currently known about endosymbiotic bacteria associated with frenulate and moniliferan siboglinids. This is particularly surprising given that frenulates are the most diverse and widely distributed group of siboglinids. Here, we molecularly characterize endosymbiotic bacteria associated with the frenulate siboglinid Siboglinum fiordicum by using 16S rDNA ribotyping in concert with laser-capture microdissection (LCM). Phylogenetic analysis indicates that at least three major clades of endosymbiotic gamma-proteobacteria associate with siboglinid annelids, with each clade corresponding to a major siboglinid group. S. fiordicum endosymbionts are a group of gamma-proteobacteria that are divergent from bacteria associated with vestimentiferan or Osedax hosts. Interestingly, symbionts of S. fiordicum, from Norway, are most closely related to symbionts of the frenulate Oligobrachia mashikoi from Japan, suggesting that symbionts of frenulates may share common evolutionary history or metabolic features.
Thornhill
Daniel J
DJ
Department of Biological Sciences, 101 Rouse Life Sciences Building, Auburn University, Auburn, Alabama 36849, USA. thornhill.dan@gmail.com
Wiley
Anne A
AA
Campbell
Amy L
AL
Bartol
Frank F
FF
Teske
Andreas
A
Halanych
Kenneth M
KM
D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics*; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics*; D042282:Microdissection; D010802:Phylogeny; D011077:Polychaeta / Q000382:microbiology*; D013559:Symbiosis / Q000235:genetics*
D004275:DNA, Ribosomal